LOCUS       DAWIVB010000001        49770 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWIVB010000001 DAWIVB010000000
VERSION     DAWIVB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 49770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.001"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="WIX60_000001"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="WIX60_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ5304342.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(2320..2410)
                     /locus_tag="WIX60_000002"
     tRNA            complement(2320..2410)
                     /locus_tag="WIX60_000002"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2374..2376),aa:Ser,seq:gga)
     gene            complement(2449..2535)
                     /locus_tag="WIX60_000003"
     tRNA            complement(2449..2535)
                     /locus_tag="WIX60_000003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2499..2501),aa:Leu,seq:cag)
     gene            complement(2571..2657)
                     /locus_tag="WIX60_000004"
     tRNA            complement(2571..2657)
                     /locus_tag="WIX60_000004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2621..2623),aa:Leu,seq:cag)
     gene            2746..5121
                     /gene="rnr"
                     /locus_tag="WIX60_000005"
     CDS             2746..5121
                     /gene="rnr"
                     /locus_tag="WIX60_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ5304343.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPVHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDVIDADAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWISDGIDHPYKAQIDTLYKLFKILQKKRFERGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKPVPIKVKKRKGKS"
     gene            complement(5460..6923)
                     /gene="guaB"
                     /locus_tag="WIX60_000006"
     CDS             complement(5460..6923)
                     /gene="guaB"
                     /locus_tag="WIX60_000006"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ5304344.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(7124..7204)
                     /locus_tag="WIX60_000007"
                     /pseudo
     CDS             complement(7124..7204)
                     /locus_tag="WIX60_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(7250..7711)
                     /locus_tag="WIX60_000008"
     CDS             complement(7250..7711)
                     /locus_tag="WIX60_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304345.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(7802..10360)
                     /gene="glnD"
                     /locus_tag="WIX60_000009"
     CDS             complement(7802..10360)
                     /gene="glnD"
                     /locus_tag="WIX60_000009"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ5304346.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQFREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSRSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQTLLEQLS"
     gene            complement(10409..10729)
                     /locus_tag="WIX60_000010"
     CDS             complement(10409..10729)
                     /locus_tag="WIX60_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5304347.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(11054..11527)
                     /gene="bfr"
                     /locus_tag="WIX60_000011"
     CDS             complement(11054..11527)
                     /gene="bfr"
                     /locus_tag="WIX60_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ5304348.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(11555..12019)
                     /gene="bfr"
                     /locus_tag="WIX60_000012"
     CDS             complement(11555..12019)
                     /gene="bfr"
                     /locus_tag="WIX60_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ5304349.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            12595..13062
                     /locus_tag="WIX60_000013"
     CDS             12595..13062
                     /locus_tag="WIX60_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ5304350.1"
                     /translation="MKIENIDIISPELFPQETFNETEAFGALVWLWAVSPIYQHAGVQ
                     EAAVNILPVLKNGQFALFSSNGHPVAYCTWAYFDEETEWQYLQSNDVLRHSENWCSGN
                     RMWLINWFAPFGDSRMMKRILVHLFPKREIRWLYHRGSEKGKRIMRFPALSKQ"
     gene            complement(13103..13458)
                     /locus_tag="WIX60_000014"
                     /pseudo
     CDS             complement(13103..13458)
                     /locus_tag="WIX60_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13455..13793)
                     /locus_tag="WIX60_000015"
     CDS             complement(13455..13793)
                     /locus_tag="WIX60_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304351.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPNPWDAASFWELKNYANPYPGS
                     ASAALDQYPSKARRRQLKDMQECGYDPIDGGKSEADACLRKKGWCRKGFDPYPENKKY
                     EWPREEGKTK"
     gene            complement(13790..14152)
                     /locus_tag="WIX60_000016"
     CDS             complement(13790..14152)
                     /locus_tag="WIX60_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ5304352.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPPPDDSAFWRLTNYAKLYPGLT
                     SATLDQYPPEERRRQLHDSFAREKKDWKECGYDPIGGGGGSEADACMRKRGWYRVGND
                     IYPENKKYEWPREEGKTK"
     gene            complement(14149..20970)
                     /locus_tag="WIX60_000017"
     CDS             complement(14149..20970)
                     /locus_tag="WIX60_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ5304353.1"
                     /translation="MNKRCYKVIFNKKRSCMMAVAENVHRDGKSMQDSEAASVRVTGA
                     ASVSSARAAFGFRMAAFSVMLALGVAAFSPAPASGIIADKSAPKNQQAVILQTANGLP
                     QVNIQTPSSQGVSVNRFKQFDVDEKGVILNNSRSNTQTQLGGWIQGNPHLARGEARVI
                     VNQIDSSNPSLLNGYIEVGGKRAEVVVANPSGIRVNGGGLINAASVTLTSGVPVLNNG
                     NLTGFDVSSGKVVIGGKGLDTSDADYTRILSRAAEINAGVWGKDVKVVSGKNKLDFDG
                     SLAKTASAPSSSDSVTPTVAIDTATLGGMYADKITLISTDNGAVIRNKGRIFAATGGV
                     TLSADGKLSNSGSIDAAEITISAQTVDNRQGFIRSGKGSVLKVSDGINNQAGLIGSAG
                     LLDIRDTGKSSLHINNTDGTIIAGKDVSLQAKSLDNDGILTAARDVSVSLHDDFAGKR
                     DIEAGRTLTFSTQGRLKNTRIIQAGDTVSLTAAQIDNTVSGKIQSGNRTGLNGKNGIT
                     NRGLINSNGITLLQTEAKSDNAGTGRIYGSRVAVEADTLLNREETVNGETKAAVIAAR
                     ERLDIGAREIENREAALLSSSGDLHIGSALNGSRQVQGANTSLHNRSAAIESSGNIRI
                     ATKDLQNTNEHLRFHTEETHREHRIEYQAEGRTERYPEGSQKELGWEIFEDESLHMRT
                     PDGSPHSVWYKYDYERITAESKITESKPGQIISGGNLVLDAAKLKNHNSRIIAGGRLI
                     VGTPESALDNDETLGTKTITDKGDLHRYHRHHKKGRDSTGYSRSPYEPAPEVSSIRMG
                     ISAYKGYAPQQASDIPGTVVPVVAENGIHPTFTLPNSSLFAIAPNNKGYLIETDPAFT
                     DYRKWLGSGYMLAALQQDPNHIHKRLGDGYYEQKLVNEQIAKLTGYRRLDGYTNDEEQ
                     FKALMDNGITIAKELQLTPGIALSAEQVARLTSDIVWLENETVTLPDGTTQTVLKPKV
                     YVRARPKDMNGQGALLSGSVVDIGSGAIENRGGLIAGREALILNAQNIKNLQGDLQGK
                     NIFAAAGSDITNTGSIGAENALLLKASNNIESRSETRSNQNEQGSVRNIGRVAGIYLT
                     GRQNGSVLLDAGNNIVLTASELTNQSEDGQTVLNAGGDIRSDTTGISRNQNTIFDSDN
                     YVIRKEQNEVGSTIRTRGNLSLNAKGDIRIRAAEVGSEQGRLKLAAGRDIKVEAGKAH
                     TETEDALKYTGRSGGGIKQKMTRHLKNQNGQAVSGTLDGKEIILVSGRDITVTGSNII
                     ADNHTILSAKNNIVLKAAETRSRSAEMNKKEKSGLMGSGGIGFTAGSKKDTQTNRSET
                     VSHTESVVGSLNGNTLISAGKHYTQTGSTISSPQGDVGISSGKISIDAAQNRYSQESK
                     QVYEQKGVTVAISVPVVNTVMGAVDAVKAVQTVGKSKNSRVNAMAAANALNKGVDSGV
                     ALYNAARNPKKAAGQGISVSVTYGEQKNTSESRIKGTQVQEGKITGGGKVSLTASGAG
                     KDSRITITGSDVYGGKGTRLKAENAVQIEAARQTHQERSENKSAGFNAGVAIAINKGI
                     SFGFTAGANYGKGYGNGDETAYRNSHIGSKDSQTAIESGGDTVIKGGQLKGKGVGVTA
                     ESLHIESLQDTAVFKGKQENVSAQVTVGYGFSVGGSYNRSKSSSDYASVNEQSGIFAG
                     GDGYRIRVNGKTGLVGAAVVSDADKSKNLLKTSEIWHKDIQNHASAAASALGLSGGFS
                     YSPKPTSGQYSTKKEAEIGKIGGKPVSLMRFDQVSAKDDELNEKYRSERIEKGETFKE
                     ANLNQNNAGGLKFGLKQNDIHSNDKYALAKMGLGNLLGNAKESESRQSITRSVISEGD
                     WQIASAQGRKNIAGIEKGTSSAHKALAKADREGLLKEVELNRDVAKEFINETLIGGIA
                     DEAYRSQFIAEHRLMTFKMDENGEPIEDKQLEEDINKQFDNSVKLKKEFASFKDYWEA
                     YKAIGGNIYELREVSDQERKNLKTARYTDPETGKTVEKIVVGVNGIFNNIQAAAKFAA
                     QQYVGRFNPEKNRYERTYENVYFLHNPETNGRGFSKLPEIAVAAFHKMLEGAKIGNKT
                     VIGLSNSGLALGNIMEDYGKDKNGLFVGSHSRGTLVVDNVLNTLNTQANRDKKILSNT
                     ELKMVGPAANVVRADKRLFQLQQGVTTPRTADFARQSIQIENHELDLIGMLIGRNPAT
                     VGTNTRQKSQWQAIRDIIGDYTSPHNCYGMANKQCVTDGYRDPENKQTQSPTGVFERG
                     VSNEIEIMYRPVRIYDLQHPKGKTK"
     gene            complement(21480..22463)
                     /gene="lipA"
                     /locus_tag="WIX60_000018"
     CDS             complement(21480..22463)
                     /gene="lipA"
                     /locus_tag="WIX60_000018"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ5304354.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(22456..23079)
                     /gene="lipB"
                     /locus_tag="WIX60_000019"
     CDS             complement(22456..23079)
                     /gene="lipB"
                     /locus_tag="WIX60_000019"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ5304355.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(23081..23356)
                     /locus_tag="WIX60_000020"
     CDS             complement(23081..23356)
                     /locus_tag="WIX60_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ5304356.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            23541..24611
                     /locus_tag="WIX60_000021"
     CDS             23541..24611
                     /locus_tag="WIX60_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ5304357.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(24808..25755)
                     /locus_tag="WIX60_000022"
     CDS             complement(24808..25755)
                     /locus_tag="WIX60_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ5304358.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(25770..26177)
                     /locus_tag="WIX60_000023"
     CDS             complement(25770..26177)
                     /locus_tag="WIX60_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ5304359.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            26258..26917
                     /gene="ung"
                     /locus_tag="WIX60_000024"
     CDS             26258..26917
                     /gene="ung"
                     /locus_tag="WIX60_000024"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ5304360.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            26914..27123
                     /locus_tag="WIX60_000025"
     CDS             26914..27123
                     /locus_tag="WIX60_000025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ5304361.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            27020..27667
                     /locus_tag="WIX60_000026"
     CDS             27020..27667
                     /locus_tag="WIX60_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ5304362.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            27740..28213
                     /locus_tag="WIX60_000027"
     CDS             27740..28213
                     /locus_tag="WIX60_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ5304363.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            28561..29505
                     /locus_tag="WIX60_000028"
     CDS             28561..29505
                     /locus_tag="WIX60_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ5304364.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTSLEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            29647..29781
                     /locus_tag="WIX60_000029"
     CDS             29647..29781
                     /locus_tag="WIX60_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ5304365.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(30071..30586)
                     /locus_tag="WIX60_000030"
     CDS             complement(30071..30586)
                     /locus_tag="WIX60_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304366.1"
                     /translation="MKSKLLLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(31052..32974)
                     /locus_tag="WIX60_000031"
     CDS             complement(31052..32974)
                     /locus_tag="WIX60_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ5304367.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAETTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGTIDLLSGSIVRSEKLNIGYFAQHQLDTIRSDQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAVAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEEAYFEENKEKLQDTLSELAKVKTQLAQIEEVWLACQE
                     ELEQIETEIEKQFAER"
     gene            complement(33051..33446)
                     /locus_tag="WIX60_000032"
     CDS             complement(33051..33446)
                     /locus_tag="WIX60_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ5304368.1"
                     /translation="MMRQRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDVKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            33619..34926
                     /locus_tag="WIX60_000033"
     CDS             33619..34926
                     /locus_tag="WIX60_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ5304369.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            34919..35353
                     /locus_tag="WIX60_000034"
     CDS             34919..35353
                     /locus_tag="WIX60_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304370.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(35412..35681)
                     /locus_tag="WIX60_000035"
     CDS             complement(35412..35681)
                     /locus_tag="WIX60_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ5304371.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(35863..38325)
                     /gene="lon"
                     /locus_tag="WIX60_000036"
     CDS             complement(35863..38325)
                     /gene="lon"
                     /locus_tag="WIX60_000036"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ5304372.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPIAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEADIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGNDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     IHVPEGATPKDGPSAGIAMTLAAVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            38576..39679
                     /locus_tag="WIX60_000037"
                     /pseudo
     CDS             38576..39679
                     /locus_tag="WIX60_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(39707..41452)
                     /gene="recD"
                     /locus_tag="WIX60_000038"
     CDS             complement(39707..41452)
                     /gene="recD"
                     /locus_tag="WIX60_000038"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ5304373.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(41520..42215)
                     /gene="lolD"
                     /locus_tag="WIX60_000039"
     CDS             complement(41520..42215)
                     /gene="lolD"
                     /locus_tag="WIX60_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ5304374.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIHAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(42208..43455)
                     /locus_tag="WIX60_000040"
     CDS             complement(42208..43455)
                     /locus_tag="WIX60_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ5304375.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            43660..43938
                     /locus_tag="WIX60_000041"
     CDS             43660..43938
                     /locus_tag="WIX60_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304376.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(43994..44611)
                     /gene="recR"
                     /locus_tag="WIX60_000042"
     CDS             complement(43994..44611)
                     /gene="recR"
                     /locus_tag="WIX60_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ5304377.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(44678..46216)
                     /locus_tag="WIX60_000043"
     CDS             complement(44678..46216)
                     /locus_tag="WIX60_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ5304378.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(46276..46650)
                     /locus_tag="WIX60_000044"
     CDS             complement(46276..46650)
                     /locus_tag="WIX60_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ5304379.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            46740..48428
                     /locus_tag="WIX60_000045"
     CDS             46740..48428
                     /locus_tag="WIX60_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ5304380.1"
                     /translation="MLQSENSKNLISCSIKALPIMISTNGITMQFGAKPLFENVSVKF
                     GEGNRYGLIGANGSGKSTFMKILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVL
                     DVVMMGHTEMWAAMTERDAIYANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLS
                     GVGISEDLHNAKMAEVAPGFKLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVL
                     NQYDSTMIIISHDRHFLNEVCTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNA
                     KAKEKLQELQEFVARFSANKSKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEAD
                     EKAKLHRQAVEVEKLAKRFETQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAY
                     NPEYSDGLLPDEGTIKWAEKASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIR
                     GTLGRLLFGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESL
                     NMALEKYNGTLIFVSHDRQFVSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            48500..49753
                     /locus_tag="WIX60_000046"
     CDS             48500..49753
                     /locus_tag="WIX60_000046"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ5304381.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT        11235 a        12775 c        13075 g        12685 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgtagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc aacgaccttg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgacacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaaca
      481 aaatcgacaa gccgtccgct cgtccgagct gggttatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttacgct tcagggttga
      601 gcggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg ctgttcgata
      661 ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt atcttgaacg
      781 gacgcatcaa acccggccaa accgttgccg tcatgaacca cgatcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gtttggaacg cgtgccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggtatcggc gtaaccatca
      961 ccgacaaaga caatcccaaa ggcctaccga tgttgagcgt ggacgaaccg acgctgacga
     1021 tggactttat ggtcaacacc agcccgctgg cgggtacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 ataccgccga tgccgacgtg ttccgcgtat ccgggcgcgg cgagctgcac ctgaccattt
     1201 tgctggaaaa catgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cgtatgaaaa cctgaccgtg gatgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggtttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccacgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggccgta
     1621 tgttcgtatc gcccaacgac aaaatctacg aaggcatgat tatcggcatc cacagtcgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgtgccagcg
     1741 gtaccgacga agccgttcgc ctgaccacgc caatcaagct gacgctggaa ggtgcggttg
     1801 agtttatcga cgatgacgaa ctcgttgaaa tcacgccgca atccatccgt ctgcgcaagc
     1861 gttacttgag cgaattggaa cgccgccgcc actttaaaaa gctggattga tgtttactgg
     1921 attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat tttttattcg
     1981 gacgggcttt gcggcttctt gaagctgttt cagacggcgt ttttcctacc caatcaagaa
     2041 actgccgcca tttttccagc ggtatatcgc cccgtcgcgt ttcggtatcg ggttcggctt
     2101 cccggcagca tgtcgaacaa tgccgtttga aggaatacgc cttttgagtc cttacaggct
     2161 gaggaagagg gtaaaacata ccgcaaaaca taccacaaaa aacggtaacg gatagttgta
     2221 agcggttgat gacgattata gacaataacg ggttttccca atgaaattat tgtttgaata
     2281 ataaaaaatc ccaaaccgta aaagtttggg atttgtattt ggcagagagg aagggattcg
     2341 aaccctcgat acgctattca cgtatacacg ctttccaggc gtgcgactta aaccactcat
     2401 ccacctctct aatggcggaa attatcccaa tcgggataat ttattatttg gtgcccggga
     2461 gaagactcga acttccacac ccatgaggat accagcacct gaagctggcg cgtctaccaa
     2521 ttccgccacc cgggcaatct aaatattgaa ataaagcaaa gcatttgatt tggtgcccgg
     2581 gagaagactc gaacttccac acccgtgagg atactagcac ctgaagctag cgcgtctacc
     2641 aattccgcca cccgggcttt gctttatttg ctgttttgcg gtacagtgtc ctgccgcaaa
     2701 gaagtcgcta ttatatagtt cataaagaga atgtcaacag tccaaatgaa taaaaatatt
     2761 aaatctttaa atttacggga aaaagacccg tttttaagtc gtgaaaaaca gcgttatgaa
     2821 catcctttgc ccagtcggga atggataatc gaattgttgg agcgcaaagg tgtgccttca
     2881 aaaatcgaat cgcttgcgcg cgagctgtcg attacggaag acgagtatgt cttttttgaa
     2941 cgccgtctga aggcgatggc gcgggacggt caggttttaa tcaaccgtcg gggcgcggtt
     3001 tgcgcggcgg acaaattgga tttggtcaaa tgccgcgtcg aggcgcataa ggacggtttc
     3061 ggttttgccg tgccgctcac gcccgccaaa gacggtgatt ttgttttgta tgaacgccag
     3121 atgcgcggca ttatgcacgg cgatattgtc actgttcgtc ctgccggcat ggaccgtagg
     3181 ggccgccgcg aagggacagt tctggatatt gtcgaacgcg cgcaaagcaa agtggtcggc
     3241 cgtttctata tggatagggg cgtggcgatt ttggagccgg aagacaagcg tctgaaccaa
     3301 agcatcgtat tggaaccgga cggcgtggcg cgtttcaaac ctgaatccgg tcaggtcatc
     3361 gtcggcgaaa ttgaggttta tcctgagcaa aaccggccgg cagtggcaaa aatcatcgaa
     3421 gttttgggcg attatgccga cagcggcatg gagattgaaa ttgccgtgcg caagcatcat
     3481 ttgccgcacc aattcagtga agcgtgtgcc aaagctgcga aaaaaattcc cgtccatgta
     3541 cgcaaaagcg atttgaaagg ccgcgtcgat ttgcgcgacc tgcctttggt aacgatagac
     3601 ggcgaaacgg cgcgcgattt cgacgacgcg gtgtttgccg aaaaagtcgg acgcaattac
     3661 cgtctggtcg tggcgattgc ggatgtcagc cattatgtcc gccctgacga tgtgattgat
     3721 gcagatgctc aagaacgcag taccagcgta tatttcccgc gccgtgtgat tccgatgctg
     3781 ccggaaaacc tgtctaacgg catttgctcg ctcaatcccg atgtcgagcg tttgtgtatg
     3841 gtgtgcgata tggtcgttac ctatgcgggc aatatcaaag aataccgctt ctaccccgcc
     3901 gtaatgcgct ctcatgcccg cctgacctac aaccaagttt ggaaatggat ttcagacggc
     3961 atcgaccatc cgtacaaagc ccaaatcgac accctttaca aactcttcaa aatccttcag
     4021 aaaaagcgtt tcgaacgcgg cgcggtggag tttgaaagcg tcgaaaccca gatgattttc
     4081 gatgacaacg gcaaaatcga aaaaatcgtc cccgttgtcc gcaacgatgc ccacaagctg
     4141 attgaagaat gtatgctggc ggcgaatgtt tgcgcagcgg atttcctgtt gaaaaacaag
     4201 catacggctt tgttccgcaa ccatttgggc cccacgcccg aaaaactcgc caccctgcgc
     4261 gagcagctcg gtctgttggg gcttcaactt ggcggcggcg acaacccgtc gccgaaagac
     4321 tatgccgcgc ttgtcgaaca attcaaaggc agacctgatg ccgaattgct gcaagtcatg
     4381 atgttgcgct ccatgcagca ggcggtttac gaaccgcatt gcgacggaca ctttggtctt
     4441 gcctacgaag catacgccca cttcacctcg cccatccgcc gctatcccga cctgaccgta
     4501 caccgcgcca tcaaagccgt gttgaatcag caaacctaca cgccaaaaaa aagctggcag
     4561 gctttgggcg tgcatacctc gttctgtgag cgccgtgccg acgacgccag ccgcgacgtg
     4621 gaaaactggc tgaaaaccta ttatatgcgc gataaggtcg gcgaagtatt cgaaggtaaa
     4681 atctccggca tgaccagttt tggtatcttt gtaacactgg acggcatcca cattgacggc
     4741 ttggtgcata tcagcgattt gggcgaagac tatttcaact tccgccccga aatcatggca
     4801 atcgaaggcg aacgcagcgg catccgtttc aacatggggg acagggttgc cgtccgggtc
     4861 gcccgtgccg atttggatga cggaaaaatc gattttgtcc tgattgccgg ggggagcggc
     4921 agggggcgga aagttaaatc atccgcgtct gccaaaccgg cagggacggc ggggaaaggg
     4981 aagccgaaaa ccgccgccga gaaaaaaaca gcccgaggcg gcaaagtaag gggaaggggc
     5041 gcgtctgccg ccgcagaatc gaggaaaaag gcaaagaaac cggttccgat taaggtaaaa
     5101 aaacggaaag gcaaatcata atgctgacgg ggtggcttga ggaggcgggg catattttcc
     5161 cgaagcctcc acccgatttt ttgatgtgag cggacggagc gttatttcca gcaaattgaa
     5221 acgcccgtat tgaaagattg cgtttatttc caccgccgtt ttaaaggccg gcggtattcg
     5281 gcagacgggg cgcaaacggc gttcagacgg cattttcatt ctttcggcgt gtccgtccga
     5341 attgctttgc ccgtccgcgc aatcagcccg tgcggcttgc cttgaacgga caaaaaatgc
     5401 cgtctgaaac ccgaaaatca ggtttcagac ggcatttttc cttgaaaagg ctgttcaaat
     5461 cagcgatggt agttcggtgc ttctttggta atttgaacgt cgtgaacgtg cgattcgctc
     5521 atacctgcgg aagtgatttc cacaaattct gctttttcgt gcatttcggc aatattggcg
     5581 caacccaaat accccatgct ggagcgcagt ccgccggtca gttggtggat gatgttcaca
     5641 atcgggcctt tgtaaggaac gcggccttcg atgccttcgg ggacgtattt gtcggtgctg
     5701 tcggttttgt cttggaagta gcggtcggca gaaccttggc tcatcgcgcc caaggaaccc
     5761 ataccgcgat aggatttgta tgagcggcct tggtagagtt cgatttcgcc cggcgcttct
     5821 tccgtgcctg caaacatacc gccgagcatg acgctgtacg cgcctgcggc gagggctttg
     5881 gcgatgtcgc cggagaagcg gatgccgcca tcggcaatca gcggaacgcc cgtgcctttg
     5941 agggcttcgg caacgttgtg aatggcggtc agttgcggca cgccgacacc tgccacgata
     6001 cgggtggtgc aaatcgatcc cggaccgata ccgactttga cggcatccgc gccggcggcg
     6061 accaaatcca aagcggcttt ggcagtggcg atgttgccgc cgatgacttg gatgtgcgga
     6121 taggtttctt tgacccaacg cacgcggtcg atcacgcctt ggctgtgccc gtgggcggta
     6181 tcgacgacaa tcacgtccac gccggcctca accaaggctt tgacgcgctc ttcggtgtcg
     6241 ccgccggtgc cgactgccgc accgacgcgc agacggcctt cggagtcttt gttggcattg
     6301 ggaaactcgg tggtttttaa aatatctttg acggtaatca gacctttgag ttcgtctttt
     6361 tcgttcagaa ccaaaacgcg ctcgactttg tgcgtgtgca tcagttcgcg cgcttcgtct
     6421 atgcttgtgc cttcggggac ggtaaccaga cgttcgcgcg gggtcataat ggcggaaacg
     6481 ggcaaatcga cgcggttttc aaaacgcagg tcgcggttgg ttacgatgcc gacgactttg
     6541 ccgttttcaa cgacgggcag gccggacatt ttgcgtttgc gctgcgcacg catttccaag
     6601 acttcgcgga tgagcgttgt cggtgcaacg gttacggggt ctttgaccac gccgctttcg
     6661 tggcgtttca ctttggaaac ggcgcgcgcc tgcatttcgg gcggcatgtt tttatggatg
     6721 atgccgatgc cgccttcttg tgccatcgaa atggcgaggc gcgcctcggt aacagtgtcc
     6781 atcgcggcgg aaagcagggg gaggttgagt gtgatttcgc gggtgagctt ggtttgaagt
     6841 ttaacgtctc gcggcagcac ggtcgaatgt gcgggaacca acaaaacatc gtcgaaagta
     6901 taggcttttt ctacgatacg cataatgctc ggtctttcag tttgtgcaag atgcacggca
     6961 ttatagcacg ttaccggcgg cttgacagtt tatcaggttt aattttggtc ccctttgaat
     7021 agctcggttt tcctttgccg accactgttg ctcccgttct ttcaatttca ggaaaagctt
     7081 ttttctaatt tttggtaagt ggctcagtta ttgaagccct atatcgggcg gtaacttcca
     7141 gcacaaaaaa acggagtagt tctttattta ttttttcctt taattttcag tatattatct
     7201 taatatttcg agggtaacat atctgctaat ctagttacag ccccatatat tatagattca
     7261 attgaaaaat aacagattca actgtacctt tcttatacct gatttcttta aagtttttcc
     7321 cattgtcaaa actataaaaa agtctctctt cctcaccaga atatcccatt tctaatacta
     7381 aacaatctat ttcacaatta ccccctccac tcatatatgt agtatcaata gaaaatatat
     7441 tgcataattc cagagaatta tatttatcta tatcaaatag ttgctctcca aaatctaaac
     7501 cattgtttcc acctgcataa tacttctctt caaaggatga cttcaaacta ttaaaaactg
     7561 catttagcgt aaaatatcca tatgggcata tcttaggata tatattctct cctattatta
     7621 tctgaatttt tccataaata aaaccattac attcagagtt ttcttcccat aaaatcccaa
     7681 attttcttgt tgagtccata ataatattca tataaatcct tatattaata aattatttac
     7741 aatatccccc gctttcagac ggcatacggc gtggcggcgg aatgccgtct gaaggcgggc
     7801 gttatgataa ttgttccagc agcgtctgct tcaatgagga ctggattttt gggtttttca
     7861 ggtcggggct aaaaacggta aaactgtctt cggcgcgttc atccagtgtg gagattttgg
     7921 catagcgcag gctgacgttg tgggcgaaaa agacttccgc catatcggcg agcaggaagg
     7981 ggcggttgac ggcggtgatt tcgacggaat accagtcggg atagtcttct tcgggggtga
     8041 tggtgatgct cggtgcgatc ggcatatagc ggctgcggcg gctgatgcgg cggctgcggc
     8101 tttgggtttc ggcaacggtg tgtccgtgga taaagctgtt gagttcggct tcgagcgcgc
     8161 tttggatgtc ggggtagtct tcgggggcgt gctgcgaggg gatttgcacg atgaaggtgt
     8221 cgaggatgta gtcgtgttcg gtgatgaagg cgcgggcggc gaggatgtcg aagccgtggc
     8281 ggctgaagat gcggcagagg cgggcgaaca ggcgcgggcc gttgggcatg aaaaccatga
     8341 cttgaaagct gtcgcttttg ggcaggatgc ggctgcggac gatgggggtt tcaaagtcgt
     8401 ggactaggtt ggcggcgtgc cacaggattt cgcggaactg gtggcgggcg aagtaggcgg
     8461 aaccgagcgc gttccatagt tttttctgct gtttttcggg gacggcggcg cgggtgagta
     8521 agtcggcggc ttcctgccgg cggcggccga agagggtgtg cgggttgccg ccgttgcctg
     8581 taaggtagcg tccggcggca tggaagaggc tttccagcag gctggcgcgc catgcgttcc
     8641 acagcttggg attggtgccg cgtatgtcgg aaatggtcag aaggtagagc gcgctgaggc
     8701 gttcgtgggt ttgcacgcgt ttgcagaagg catcgagtac gctggggtct tggatgtctt
     8761 ctttttgggc gacggcagac atcagcaggt ggttttcgac cagccaggcg agcaggtcgc
     8821 tttcttctcc ggtcaggaag tggtcagcgg caaattggcg cgcgtctgcg atgccttgta
     8881 tggcatggtc gccgccgcgt cctttggcga tgtcatggaa aaaggcggca aggtagagga
     8941 tgtcttgttt ttcaaaggac tgcatcagtg cagaggcgta gggcagctcg tggctgtgca
     9001 tatccaaggc aaggcggcgg acgttgcgga cgacggtgag gatgtggtcg tccacgggat
     9061 agatgtggaa caggtcgtgt tggagcaggc cgatgatttt ttcccacgcg ggcaggtagc
     9121 ggcccaacac gccgtagagg ttgagaaagc gcagggtctg ggtcagcccg ttgccgttgc
     9181 ggaaaaaacc ggcgaagcgg cggcggtttt cgggattttg gtagaagctg cggttgattt
     9241 tgcgcgtcgc gccccaccag gcgcgcaggg tttgcggttc gagcgcggta atgtcgttgc
     9301 gctgctgcat gatttcgacg attttgaaaa tgtgttcggg ccgtctgaaa aaaatatcgg
     9361 tgtgccgcgc ggcgatttgg ttgttgactt ggatgtagtc gtcgtcaatc cgcagggtaa
     9421 cgcgcaacgg ggtggaggaa acgcggcttt gcagcatagg cgtgaggatg ccgcccagtt
     9481 gtttgacggt tttaatcgcg cggtaaaaca cgcgcatcag ttcttcgctt tggcggcgga
     9541 ggttcaagcc ttcataaccc atgctttcgg cgacttgcgg ctgcaaatcg aacagcaggc
     9601 ggtcttcggc gcgcttggcg tttaaatgca gatggatgcg gatgtgggcg aggcggcggt
     9661 agccgtgcga aagcataccg gcttcggcac gcgtcaaaat ccgctgtttg agcaggtcgg
     9721 gcaggtcggt cgccaagcct tgcgccttcg ctatccaaag cagggtgtgg atatcgcgca
     9781 gaccgcccgg acagcttttg atattcggct ccaatactgc ccccgaacct tgcgatttgg
     9841 cgtggcggtg ttccatctcc accagttttg cctcgacaaa cgccgccaca ttgcgctgcg
     9901 cgttcatttt ttccgccagt ttatccaccg tttggcggtt gccaaacaaa aacctagcct
     9961 ctaaaaacgc tgtgtccccc gtaatatcat tgcgcacgct ttcacatagt tcatcaacgc
    10021 tgccgctttt tacagacggc atcagtttgc agtcccacag ggtttgaaca aaccgggcaa
    10081 tctgttcctg aatgccgtct gaaagcgggg caggggagac aaccgccaaa tccacatccg
    10141 aacagggata cagttcgccg cgtccgaagc cgcctaccgc catcaggcat aacgcgctgt
    10201 tttgaaaata ctctgcccac aatgccgcca gtaaggtttc gactgccgcc gtgtattctc
    10261 tgaaaaatac cgacacgcgg ttggctttca aataatgcgc ttcggcggca tcgcgctgct
    10321 gtttgaaggt ttccagtgct gaagacaggt ttgcaggcat ttttattctt tcgattggcg
    10381 ggaaaaaggg aggcggatgg ttcggcggtc aaataccgct ttcagacggc atttgtcggg
    10441 tatcggcgga attggtcatc agcttcaacc gttcctgcgg cggcagcagc aactgatacg
    10501 ccgccacgcc caaagccgcc gccatttttt cgatattcga cagggcgatg ttccagcgtt
    10561 tgcgctcgac tgccgacaca taagtcctgt ccaaaccgca ttgccgcgcc aattcttctt
    10621 gcgaccaacc cttgttcacg cggaaaagcc gcatattgta tgccaatacc gcccgcaaat
    10681 cctgttcgtc aggcagttcg gcaggcagag tcaatttgtt gcccatcatt tgcttccaga
    10741 taaatggtta aagttaagca tttgctgtta cggattttac ttcacataaa agccaaaaaa
    10801 aaggggtggc ggcagccaca cccaaacaca cacacatcaa gaggaaagag ataaaatcaa
    10861 gacagattgg gaaacagcaa acaagcaccc tgaatttcat atcggtatta tcaaacaaaa
    10921 acccttatca ggtattgatt caaatcaatt tagattttat tcgcaaaccg aaaaagaaaa
    10981 acggcatatc cgtttatacg gatatgccgt ctgatggtgc gggggactgc tgtatttggc
    11041 agtggtttgt gttttagtcc tcttgcgccg ctgtttgcag gtagttcggc agaccgattt
    11101 tgccgataag ctcttgctgg gtctccagcc agtctatatg ttcttcgttg gtgtcttttt
    11161 gtttttccaa caaatcgcgg ctgacgtaat cctgttgcgc ttctgctgtg gcgatggcgg
    11221 caagcagggc ttcgtgtttt tcctgttctt tggtcaaatc gcaggcgatg atttcttcgg
    11281 tggactcgcc aatcagaagc ttgcccagtt cttgcaggtt cggcagacct tcgaggaaca
    11341 gaatgcgctc gatcaaatcg tcggcagctt tcatttcaac gatggactgt ttgaagaaat
    11401 gttcgcccag ttcttcaaag ccccagtttt tcaaaatacg ggcgtgaagg aaatattggt
    11461 tgatggttac cagcagcaag cctaagtttt tgttcagctc gcggataacc aaacggtcgc
    11521 ctttcatacg gactcctttt attccgaata cggattacag ttggctttgg tagtagttgc
    11581 cttcgccaat taactcgatc aggcgaagct gctgttccag ccagtgggcg tggtcttctt
    11641 cggtatcttt cagttgggca accatcaggt cgcgcgtaac atagtcttga gcctcttcgc
    11701 acagtttgat gccttttttc aacgcatcac gtacttcata ttcggtttgc aggtcggctt
    11761 tgaggcagga aaccacgtcc gtgccgatat tcagttcggc gcgtgccatt ttcggcgtac
    11821 cgcccagcat caggatgcgg cggatgaagt cttcggcgtg tgtggtttct tcttccatct
    11881 cgtggttgag acgttcaaaa agtttggtgt agccccattc ggagtagagg cgggagtgga
    11941 taaagtattg gtcgcgtgcc gccagctcgc cagacagcaa ttcgttcata taatcaacaa
    12001 cagcttgatt gccttgcata atatctctct ttctgtaact tggttttcgg tatgtctgaa
    12061 cagcatctgt gtcgctattc gggatgcgtg cattgtatgc aaaagctgtc ggcatgacaa
    12121 attttctatt taaaatacaa acaattatca aaagaaatag ggcgtattcc ccgatgcctt
    12181 ccaaatcagt atgctgtttc ttatcggttt ttttgctgct aaaactaaga atccatctca
    12241 tccataaaga acatttacac ttattaacca tagcaaaaaa caaatagagc acggtttttt
    12301 atcaaaattt ataatgaatc gttctcatta accgacagat tcttttgaga ttatcggatt
    12361 ttgggaataa attatgccgt ctgaggggct ttcagacggc atgcctgccc ggacagacgc
    12421 tgcttggagc ggctgtcggt ttgaaggtgt gtttaatgcg aagggcaatc tgtcgtatga
    12481 tgtgtttgcg gcaggggctt tgaagaagcc cggtatttta ggacgaagcg cgcggctgcc
    12541 ggggtcaata tccgctattt attccaaacc ttattaaaac ttaaatttaa aatcatgaag
    12601 atagaaaata tcgatattat ttcgccggaa ctgttcccgc aggaaacatt caatgaaacc
    12661 gaagcattcg gcgctttggt atggttgtgg gcagtttcgc ctatttatca gcatgccggc
    12721 gtacaagaag ccgccgtcaa tatcttgccg gtattgaaaa acgggcaatt tgccttgttc
    12781 agcagcaacg gacaccccgt cgcctactgc acttgggctt atttcgatga agaaaccgaa
    12841 tggcaatatc tccaatccaa tgatgttctg cgccactcgg aaaactggtg cagtggaaac
    12901 agaatgtggc ttatcaactg gttcgcacct ttcggcgaca gccgtatgat gaaaagaatt
    12961 ctggtgcatc tgtttccgaa acgtgaaata aggtggctgt accaccgggg gagtgaaaaa
    13021 ggaaaacgga ttatgaggtt tccagcgttg tcgaaacaat aaaacaaggc tgcctgaaag
    13081 tttcccttca ggcagccttg tttcagttct ctcgaggcca ttcgtatttt ttgttttcgg
    13141 gataaatgtc gtcacctaca cgataccaac ctctttttct catgcaggca tctgctttac
    13201 ttcctcctcc cccacctatt gggtcatagc cgcactcttt ccagtctttt tttccttagc
    13261 aaaaagatca tctatttgtt tgctatattc ttcataagaa tatatgcgta aattgatatt
    13321 tttactcatc attgccggat attcttgttc tatacgggaa tacttccagt ataccgagtc
    13381 atcgggcgga ggtttgaatc ccccaaacga acaggcagcc aatcccatag ccaaagagat
    13441 tgatacgata tatttcattt tgtttttcct tcttctcgag gccattcgta ttttttgttt
    13501 tcgggataag ggtcgaaacc cttacgacac cagccttttt tcctcaggca ggcatctgct
    13561 tcagactttc cgccgtctat tgggtcatag ccgcactctt gcatgtcttt cagttgcctt
    13621 cgtcttgctt tcgatggata ttggtcaagt gccgccgagg ctgatcccgg atagggattg
    13681 gcgtaatttt tcaattccca aaatgacgcg gcgtcccacg gatttggttt aaatccccca
    13741 aacgaacagg cagccaatcc catagccaga gagattgata cgatatattt cattttgttt
    13801 ttccttcttc tcgaggccat tcgtattttt tgttttcggg ataaatgtcg ttacctacac
    13861 gataccaacc tctttttctc atgcaggcat ctgcttcact cccaccgccc ccacctattg
    13921 ggtcatagcc gcactctttc cagtcttttt tttctctagc aaaagaatca tgcagttgtc
    13981 ttcgtctctc ttcaggtggg tattggtcaa gtgtcgctga ggttaatccc ggataaagtt
    14041 tggcgtaatt tgtcaatctc caaaatgccg agtcatcggg cggaggtttg aatcccccaa
    14101 acgaacaggc agccaatccc atagccagag agattgatac gatatatttc attttgtttt
    14161 tcctttcgga tgttgcagat cataaatgcg tacaggacgg tacataattt cgatttcgtt
    14221 ggacactcct ctttcaaata cacctgtcgg actttgggtt tgtttatttt cagggtctcg
    14281 ataaccatct gttacacatt gtttattagc cataccgtag caattatgcg gcgaggtgta
    14341 atcccctata atatccctta tagcctgcca ttggcttttt tgccgggtat tggttccgac
    14401 tgttgccgga tttctgccaa tcagcatgcc gataaggtct aattcgtggt tttctatttg
    14461 gatggactga cgggcgaaat ctgccgttct gggtgtcgtt accccctgct gcagttgaaa
    14521 cagcctttta tctgcccgga caacgttggc ggcagggccg accattttca gttcggtatt
    14581 ggataaaatt ttcttatccc gattggcttg ggtattcaag gtattgagta cattgtcgac
    14641 caccagggtt ccacggctgt gcgagccgac aaacaggccg tttttatcct tcccgtagtc
    14701 ttccatgata ttgcctaaag ccaagcctga attgctcaag ccgataacgg tcttattgcc
    14761 tatttttgcc ccttcgagca ttttatgaaa ggccgcaacg gcgatttcgg gcaattttga
    14821 aaagccccgc ccattggttt ccggattgtg caggaaataa acattttcat aggtgcgttc
    14881 atatcggttt ttctcgggat tgaaacgccc cacatattgt tgggcggcaa atttggcggc
    14941 tgcttgtata ttattgaaaa tgccgttgac gccgacaacg attttttcaa cggttttgcc
    15001 ggtctccggg tcggtgtaac gggcagtttt cagatttttc cgttcctggt cggatacttc
    15061 gcgcaattcg tagatatttc ccccaatggc tttataggct tcccaataat ctttaaatga
    15121 ggcaaattct tttttcaact ttactgagtt gtcaaactgt ttatttatat cctcttcaag
    15181 ctgcttatct tcaatcggct cgccgttttc atccatttta aaggtcatca ggcggtgttc
    15241 tgcaataaac tggctgcggt aggcttcgtc tgcaatgccg ccgatcagcg tctcattgat
    15301 aaactctttg gcaacatccc tattgagttc gacttctttc agcaagcctt cgcggtctgc
    15361 ttttgccaat gccttatgtg cggatgaagt gcctttttca ataccggcaa tatttttcct
    15421 gccctgcgcg gaagcaattt gccaatctcc ctcactgatg acggagcggg taatggattg
    15481 gcggctttct gattctttgg cattgcccaa cagattgccc aaacccattt ttgccaaggc
    15541 gtatttgtcg ttgctgtgta tatcgttttg cttcaaaccg aatttcaagc cgccggcgtt
    15601 gttttgattt aaatttgcct ctttaaaggt ttcccctttt tcaatacgct cacttcgata
    15661 tttttcgttc aactcgtcgt ctttcgccga aacctggtca aaacgcatca ggctgaccgg
    15721 tttgccgccg attttaccga tttctgcttc ttttttggtg gaatactgtc cgctggtagg
    15781 cttcgggctg tatgaaaaac cgccgctcaa gcccaaggcg gaagcagccg ccgaagcatg
    15841 gttttgaata tctttatgcc agatttcgct tgttttcagc aggttttttg atttgtcggc
    15901 atctgaaaca acagcggcgc cgaccaatcc ggttttgccg tttacgcgaa tccgatagcc
    15961 gtctcctcct gcaaagatac cgctttgctc gttgacggat gcataatccg agctgctttt
    16021 cgagcggtta tagctgccac cgacactaaa gccgtagcct accgtaactt gggcggaaac
    16081 attttcctgt ttgcctttaa acacggcggt atcctgcaaa ctttcgatat gcagactctc
    16141 tgccgttacg ccaacgcctt tgcctttaag ctgcccgcct ttgatgacgg tatcgccacc
    16201 gctttcaata gcggtttggc tgtctttgct gccgatatgg ctgttgcggt aggcggtttc
    16261 gtcgccgttg ccgtaacctt tgccgtagtt tgctccggct gtgaagccga aactgatgcc
    16321 tttgttgatg gcgatggcga ctccggcatt aaagcctgcg gatttgtttt cgctgcgttc
    16381 ctgatgcgtt tggcgggcgg cttcaatctg aacggcattt tctgctttga ggcgtgttcc
    16441 tttgccgccg tacacatcgg agccggtaat cgtgatgcgg gagtctttgc ctgcgcctga
    16501 agcagtcagg gaaactttgc cgccgccggt gattttgccc tcttgcacct gcgtgccttt
    16561 gatgcggctt tcggaggtgt tcttctgttc gccgtaggta acggagacac tgatgccctg
    16621 accggctgct tttttgggat ttcgggcggc attatagagt gccacgccgg aatctactcc
    16681 tttattcaag gcgttggcag cagccatggc attgacccgg ctgtttttgc ttttgccgac
    16741 ggtttggact gcttttacgg cgtcaaccgc gcccattacg gtattcacaa ccggaacgct
    16801 aatggcgacg gttacgcctt tttgttcgta aacctgctta ctctcttggc tgtaacggtt
    16861 ttgtgcggca tcgatgctga tttttccgga ggaaatgccg acatcgcctt ggggcgagga
    16921 tatggtcgaa ccggtttggg tgtaatgttt tcctgccgaa atcagggtat tgccgttcag
    16981 gctgccgacg acgctttctg tgtggctgac ggtctcggat cggttggttt gcgtgtcttt
    17041 tttgctgccc gccgtaaagc cgatgccgcc gctgcccatc agtccggatt tttctttttt
    17101 gttcatttcg gcactgcggc tgcgtgtttc ggctgcttta aggacgatat tgttttttgc
    17161 cgagagaatg gtatggttgt ctgcaatgat attgctgcca gtaacggtaa tatcgcgtcc
    17221 tgaaaccaga atgatttctt tgccgtccag cgtgccggat acggcttgtc cgttttggtt
    17281 cttgagatgg cgggtcatct tctgtttgat gccgcccccg cttctaccgg tgtatttcag
    17341 ggcatcttcg gtttcggtat gggctttgcc ggcttcgact ttgatatccc gtccggctgc
    17401 cagtttcaga cggccttgtt cgctgccgac ctctgctgca cggatacgga tgtctccttt
    17461 tgcattcaga ctgagattgc cccgggtgcg gatggtgctg ccgacttcgt tttgttcttt
    17521 gcgaatcaca tagttgtcgg aatcaaagat agtgttctga ttgcgggaaa tgcccgtcgt
    17581 atccgagcgg atgtcgccgc cggcattcag tacggtttga ccgtcttcag attgattggt
    17641 caattcggaa gccgtcagga cgatattgtt gcctgcatcc agcaagacgc ttccattctg
    17701 cctgccggtc agataaatgc ctgccacccg gccgatattg cgtaccgagc cttgctcatt
    17761 ctgattgctg cgggtctcgc tgcggctttc tatattgtta ctcgctttga gcagcagggc
    17821 gttttctgcg ccgatgctgc cggtattcgt aatgtcgctg cctgctgcgg cgaagatgtt
    17881 tttgccctgc aaatcacctt gcagattttt aatattctgt gcgtttaaaa ttagtgcttc
    17941 gcgcccggca attaagccgc cccggttttc aatggcgccg ctgccgatat caacaacgct
    18001 gccggacagc aacgcccctt gtccgttcat atctttgggg cgtgcgcgga catagacttt
    18061 gggtttcaat acggtttgag ttgtcccgtc gggcagggta acggtctcgt tttccagcca
    18121 aacaatgtcg gaagtcagac gggcaacctg ttcggcagac agggcaatac ccggagtaag
    18181 ctgcaattct ttggctatgg taatgccgtt atccatcaaa gccttgaatt gctcttcgtc
    18241 attggtataa ccgtccaagc ggcggtagcc tgtcagcttg gcgatttgtt cgtttaccag
    18301 tttctgctcg taatagccgt cgcccaaacg cttgtggata tggttcgggt cttgttgcag
    18361 tgcggcaagc atatagccgc tgcccagcca tttgcggtag tcggtaaagg cagggtcggt
    18421 ttcaatcaaa tagcctttgt tgtttggcgc aatggcaaac aagctgctgt tcggcagagt
    18481 aaatgtagga tggatgccgt tttcagcaac aacgggtaca acagtgccgg gtatatcaga
    18541 tgcctgttgg ggcgcatagc ctttataggc tgaaataccc atacggatgg agctgacttc
    18601 gggggccggt tcgtagggag accgactgta tcccgtagaa tcccgccctt tcttgtgatg
    18661 acggtggtaa cggtgcaagt cccctttatc ggttatggtt tttgttccca aagtttcatc
    18721 attgtccaat gcggattcgg gcgtaccgac gatcaatctg ccgccggcaa taatccggct
    18781 gttatgattt ttcagcttgg cggcatctaa aaccaaattg ccgccactga tgatttgacc
    18841 gggcttggat tcggtaattt tgctctcggc ggtgattcgt tcgtaatcgt atttgtacca
    18901 gacagagtgg gggctgccgt ccggagtccg catatgtaaa gactcatctt caaatatttc
    18961 ccagcccaac tctttttgag aaccttccgg atagcgttct gttctgcctt ccgcctgata
    19021 ttcgatacgg tgttcgcgat gggtttcttc cgtatggaaa cgcagatgct cattggtatt
    19081 ctgcaaatct tttgtagcga tacggatatt gcctgatgat tcgattgccg cactgcggtt
    19141 gtgcagtgat gtatttgctc cttgcacctg tcggcttcca ttcaatgcag aaccgatatg
    19201 aagatcgccg gagctggaca ataatgccgc ctctcggttc tcaatttccc gcgctccaat
    19261 atccaaccgc tcccgcgctg caattaccgc cgctttggtt tcgccgttga ccgtttcttc
    19321 ccggttcaac aaggtatctg cctcaactgc cacacggctg ccgtaaattc tgcccgtgcc
    19381 ggcattgtcc gacttggctt cggtttgcag cagcgttata ccgttgctgt tgattaaacc
    19441 cctgttggtg atgccgtttt tgccgtttaa cccggtgcgg tttccggatt ggattttacc
    19501 ggagacagtg ttgtcgattt gggcggcggt cagggatacg gtatcgcctg cctgtatgat
    19561 gcgggtgttt ttcagacggc cttgggtgga gaacgtgagt gtgcgtccgg cttcaatatc
    19621 gcgtttgccg gcaaaatcat catgaagaga aacggaaaca tcgcgtgcgg cggttaatat
    19681 gccgtcgttg tccagtgatt ttgcctgtaa ggaaacatct ttgcccgcaa taatcgtgcc
    19741 gtctgtgtta ttgatatgca ggctgctttt gcctgtatcg cgaatatcca gcaaaccggc
    19801 cgagccgatt aagcctgctt gattatttat gccgtctgaa accttcaata cactgccttt
    19861 gccgctgcgg ataaagcctt ggcggttatc aacggtttga gcggaaatgg tgatttcggc
    19921 agcatcaatc gaaccgctgt tgctcaattt gccgtctgcg cttaacgtaa cgccgcctgt
    19981 tgcggcaaaa atccgaccct tgttgcggat tacggcgccg ttgtcggtgc tgattaaagt
    20041 gattttgtct gcgtacatcc cacccagtgt ggcggtgtcg atggcaacgg taggagtaac
    20101 agaatccgaa gaagatggcg cagaagctgt tttggcaaga gagccgtcaa aatccaattt
    20161 gttcttaccc gaaaccacct tgacatcttt accccaaacg cccgcattga tttcagcagc
    20221 acgactaagg atacgggtgt aatcggcatc agaggtatcc aaacctttgc ccccaatcac
    20281 gactttaccc gaagaaacat caaagcccgt cagattgccg ttattcaaaa caggaacgcc
    20341 cgaagtcagc gtaaccgaag cggcattaat caatccgccg ccattcacac ggatgcccga
    20401 cggattggca acgactactt cggcgcgttt gccgccgact tcgatataac cgttcaacaa
    20461 cgaaggatta ctgctgtcaa tctggttcac aattacccgc gcttcgccgc gtgccagatg
    20521 gggattgcct tgaatccatc caccgagttg cgtttgcgta ttgctgcggc tgttgtttag
    20581 tattacgcct ttttcatcaa catcgaactg cttgaatcgg ttaacagaaa cgccttggga
    20641 tgacggagtt tgaatattga cttgcggcaa accgtttgct gtctgaagaa taacggcttg
    20701 ttggttttta ggggcggatt tgtcggcaat gatgccggaa gcaggggcag gggaaaacgc
    20761 agcaacaccc aaagccaaca tgacagaaaa ggcagccata cggaaaccga aggctgcccg
    20821 ggcagaagaa acagaagcgg caccggtcac tcgaaccgaa gccgcctcac tatcctgcat
    20881 actcttgccg tcacgatgaa cattctctgc tacagccatc atacaactgc gtttcttgtt
    20941 gaagataacc ttgtagcatc gcttgttcat gatgggtttt cttaaatgaa atgtaaggaa
    21001 tagttaagga caaaatatag gaaatttgaa ttaaattgtc aataaaaaac tgacagcgta
    21061 ccctgataat cagatgttgt acgctaattg tgagaagtat gtcgggatga ttattttgaa
    21121 gttttctttt tattcaaaag agttactatg aaagttaacc gggcgtaaat aggcttcaat
    21181 aaacaggtta tcgttatttt tcaattggga aaaaatcttc cctaacccat ccattcgcca
    21241 aattattatg tgggaattcg gcaatttcaa taacggctgt tgatccatat ctgctcggtc
    21301 atttagcgtt ataaagtcgc aaatagcgtc agaatttaca agattatcgg attttgggaa
    21361 taaattatgc cgtctgaagg gctttcagac ggcataggcg gctaacacgg gtgcggcttg
    21421 cgccaaacgg cgcggcaggc gcagggaatc gctttttact ttggcgcggt gttccgggtt
    21481 taatgatgcc cgcaaccgcc gtggctttcc ctcaaggctt ccgccgcctg ttcgtcggcg
    21541 tggtagctgg aacgtaccat cgcgccgatg gcggcattgc tgaagcccag ttcgtatgct
    21601 tctttttcaa agattttgaa ctgctcgggc gtaacgtagc gcaggacggg caggtgtccg
    21661 tctgaaggct ggaggtactg tccgatggta atcatttcga tattgtgcgc ccgcatatcg
    21721 cgcataattt cacgcacgtc ttcgtctgtt tcgcccaagc cgaccatgat gccggatttg
    21781 gtcgggatgt gcggcatcat ttctttataa cgttttaata agtctaaaga atgttgataa
    21841 ttggcaccgg gacgggcttt tctgtacagg ctcggatggg tttctaggtt gtggttcatc
    21901 acgtcgggcg gggtttcggc aaggattttg agtgcgatgt ccaagcgtcc tcggaagtcg
    21961 gggacgagga tttcgatttt ggtgttcggg ctggtttcgc ggatggcttt gatgcagtcg
    22021 gcgaaatgct gtgcgccgcc gtcgcgcagg tcgtcgcggt cgacggaggt gatgacgacg
    22081 taacgcaggt tcatggcttt gacggattcg gcgaggtttc tcggttcgtc ggggtcgagc
    22141 atattgggcc gaccgtgtcc cacgtcgcag aacgggcagc ggcgggtgca gatgtcaccc
    22201 ataatcatga aggtcgccgt gcctttgctg aagcattcgc cgatgttggg gcaggaggct
    22261 tcctcgcaaa cggtgtgcat cttttgttcg cgcaaaatgt ctttgatttc aaagaatttg
    22321 cgcgatggga gtttggcgcg tatccattcg ggctttttca gtttttcctg aagggggacg
    22381 actttgatgg ggatgcgcgc ggttttgtcc gcgcctctga gtttgatgcc gcgtttgggg
    22441 tcgtcggttt tgatttcact cattgttgtc tgctttcggt gtgaattgtg tttcaaggtg
    22501 tgcggtgagt ttggcggcga cttcgtccgg cgtggggcag ggttggacaa aatccgcgat
    22561 ttgcgtcatt tccataccgg cgtagccgca ggggttgatg tgggtaaacg ggcttaaatc
    22621 catattgacg ttgagcgcaa gcccgtgata gacggagccg tttttgatac gcagccccag
    22681 tgaggcgatt ttgcgttctc cgacataaac gccggggcgt ttggggtctg ccgccgcttc
    22741 gatgccgtat tctgccaatg tggcgatgat gctgttttca agcgcggaaa cgatgtttct
    22801 aacactggtt ttgcgccgtt tgaaatcaat catcgtataa acgaccaatt gcccgggccc
    22861 gtgataggta atctgcccgc cccggtcgat ttggacgacg ggaatgtcgt cgcgaatcag
    22921 caggtgttcg ggttttcccg ccagtccttg tgtgaacacg ggcgggtgtt cgacgaccca
    22981 cagttcgtct tcggtgtcgg cattccgtcc ggcattaaag gttttcatcg cttcaaaagt
    23041 cggcagatat tcgaccaaac ctttgtgtat gattttcatc tcaaagtacc actttgacca
    23101 gttcgtgcga agtcagcgca cggtagatgt tgtccaattg ttcttggttt tcaaccttta
    23161 cctgtacggt ggcgccagta tagttgcctt tgctgctcgg acgcgtggtg atgtggtgcg
    23221 cctgcgtgtc gggggcgtgg aggcggacgg tgtctaaaac cgcctgctcg aactcgggat
    23281 gcaccgcgcc cattactttc aatgggaagg tgcagggaaa ttcgatgagg gatgttttgt
    23341 ttttttgttc ggtcatgatg tgctgccttg tcgtgtacgg tatgccgtct gaaggcgggt
    23401 ttgcctttca gacggcatcg gatgtgcgtt attttagcct aaaccgcgat aacaggctat
    23461 cgggaaggcg gaggcttttt tgacggcgcg gcggttctgc tatactggcg cacaatatta
    23521 tttttagaag ggtggttttt atgtatcgga ggaaagggcg gggcatcaag ccgtggatgg
    23581 gtgccggtgc ggcgtttgcc gccttggtct ggctggtttt cgcgctcggc gatactttga
    23641 ctccgtttgc ggttgcggcg gtgctggcgt atgtattgga ccctttggtc gaatggttgc
    23701 agaaaaaggg tttgaaccgt gcatccgctt cgatgtctgt gatggtgttt tccttgattt
    23761 tgttgttggc attattgttg attatcgtcc ctatgctggt cgggcagttc aacaatttgg
    23821 catcgcgcct gccccaatta atcggtttta tgcagaacac gctgctgccg tggttgaaaa
    23881 atacaatcgg cggatatgtg gaaatcgatc aggcatctat tattgcgtgg cttcaggcgc
    23941 atacgggaga gttgagcaac gcgcttaagg cgtggtttcc cgttttgatg aggcagggcg
    24001 gcaatattgt cagcagtatc ggcaacctgc tgctgcttcc cttgctgctt tactatttcc
    24061 tgctggattg gcagcggtgg tcgtgcggca ttgccaaact ggttccgagg cgttttgccg
    24121 gtgcttatac gcgcattaca ggcaatttga acgaggtatt gggcgaattt ttgcgcgggc
    24181 agcttctggt aatgctgatt atgggcttgg tttacggttt gggattggtg ctggtcgggc
    24241 tggattcggg gtttgccatc ggtatgcttg ccggtatttt ggtgtttgtc ccttatctcg
    24301 gggcgtttac gggattgctg cttgccaccg tcgccgcctt gctccagttc ggttcgtgga
    24361 acggcatcct atcggtttgg gcggtttttg ccgtaggaca gtttctcgaa agttttttca
    24421 ttacgccgaa aatcgtggga gaccgtatcg ggctgtcgcc gttttgggtt atcttttcgc
    24481 tgatggcgtt cgggcagctg atgggctttg tcggaatgtt ggcgggattg cctttggccg
    24541 ccgtaacctt ggtcttgctt cgcgagggcg tgcagaaata ttttgccggc agtttttacc
    24601 ggggcaggta ggcggttccg aaacatatag tggattaaca aaaatcagga caaggcgacg
    24661 aagccgcaga cagtacaaat agggcaacgc cgtactggtt tttgttaatc cactatattt
    24721 gaagcggaat acaaccttgc ccgggtttaa ataaaaatgc cgtctgaacc ccgaaaactg
    24781 gacttcagac ggcattttca tcacggctta tttggcggtt ttgctgctgt cgataatttt
    24841 cataccggca gaaatcaggc tgccgatgtc ggcaacattg gcgggcataa tcagcgtatt
    24901 gctttctttg gcaagattgt tgaacgcagc gacgtattgt tccgcaatct tcagattgac
    24961 cgcatccgca ccgccttggg tttgaagggc ggcggcaatt tgacggatgg cttcggcatt
    25021 ggcttcggca acaaggcgca aggattccgc ttcacctttg gcgcggttga tgcgggcgat
    25081 tttctcggca tttgacgcat tgaccgcagc ctgagcctcg ccttcggatt gttggatttc
    25141 ggcttcgcgc tgaccactgg caaggttgat ttgttcgatt ttacgacctt cggattcggc
    25201 gatacgggcg cgtttttcgc gttcggcagt aatttgcgcc tgcattgagc gaaggatttc
    25261 ttgcggcgga accaagtctt taatctcata acgcaaaacc ttcacacccc aagccccggc
    25321 cgcctcgtcc aaagccgcaa caacagtact gttgatttcg tcgcgttctt caaacgtttt
    25381 gtccaactcc atacgcccga taacggaacg cagcgtcgtt tgggcaagct gggtaatcgc
    25441 cataatgtag ttgctcgaac cgtatgaggc gagtttgggg tcggttactt ggaaatagat
    25501 gatgccgtca acagtcagct gcgtattgtc gcgcgtgatg cagacctggc tgggtacgtc
    25561 taaagggatt tctttcagcg aatggcggta ggcgacgcgg tcgataaagg gaatcaaaat
    25621 attcaaaccg gccgtcaggg cgcgatggaa acgccccagc ctttcgacaa cgtggacttc
    25681 ctgttgtggg atgacaacaa aggatttgaa accgaaaacg gcgacggcta ccaacaagat
    25741 aatgaaaaat tccataattc ctccgagtgt taagggtgtg tgataataag aaggttgcct
    25801 tccttgcgga caatgagggc gcgagttcct ggttcaagct cttcttgccc cgtattttga
    25861 gcctgccagt gcgtaccgcg ataaaaaact tcgtaacggt tgccgcctgt gtgtcggagg
    25921 atttcgacat attgtccggc atccaaatcc tgatatgaat ccgtttcaac ttttctaacg
    25981 gcggttttgg cgtgtacgaa ccaaataccc agcgcggaaa gcagagcggc ggtcaagacg
    26041 gcggcaggcg tactgccggt cagcccgtaa gcaatgcccg aacccgccaa agccgcgctg
    26101 acaaccaaaa gataaaccgt tcccgtcaat aattcgatga ttaagacggc aacagcggca
    26161 acaaaccata cagtcataca tttccccaca aagcgcgtcg tttgacaaaa taacgcaata
    26221 tcagcagtat agccgaattt gaaaggatag ggcagatatg gacacttggc acgatgcact
    26281 cggcggcgaa aaacagcagc cgtattttca ggaaatttta aatgcagtca ggcaggaacg
    26341 tttgtcggga caaatcatct atccgccggc ggcggatgtg ttcaacgcat tccgcctgac
    26401 agcgttcgac cgggtcaaag ccgtcattct cggacaagat ccgtatcacg gggcagggca
    26461 ggcgcacggt ttggcatttt ccgtccggca gggtatccgc ataccgccgt ctttactcaa
    26521 tatctacaag gagttggaaa ccgacatcga aggcttttcc attcccgcgc acggctgtct
    26581 gacagcgtgg gcggagcagg gcgtattgct tctgaacacg gttttgacgg tgcgtgcagg
    26641 acaggcgcat tcgcacgccc ttttaggctg ggaacgcttt accgataccg ttatcaggca
    26701 gcttgcgaca caccgcaagc accttgtctt catgttgtgg ggtgggtatg cacaacaaaa
    26761 agggaggctg atagacagtc aaaatcattt gatattgacc gcaccgcatc cgtctcctct
    26821 gtcggcatat cgcggttttt tcggctgccg ccatttttca caggcaaaca gctatttgag
    26881 ccggcacggt atcgatccga taaactggaa gctgtgaatg ccgatatagc cgttgccgcc
    26941 ggcgtgttaa aatcgcgttt gatttgtaat ttccatttat taggcaaaac cttatggcaa
    27001 tcaaaaaaaa atcagcttta tgcatcactt tgggcgggct gcgacgagtt gcgcggcggt
    27061 atggatgcga gcctttataa agaactatgt gcttacgcta ttgtttttaa aatatgtttc
    27121 tgataagcat aagtacggcg gcggcatgat tgagctgcac gccggtacga cttttgacga
    27181 catcgtcaaa ctcaaaaaca ccgccgacat cggcgaccgc ctgaataaga ttatcgccca
    27241 aattgccgaa gccaacgact taaaaggcgt gatcgacgtt accgacttca acgacgaaga
    27301 caaactgggt aaaggtaagg agatgatcga ccgtttgagc aggcttgtcg gcatttttaa
    27361 aaagctcaac ctttcttcca accaagccga agacgacgat ttgttaggtg atgcctacga
    27421 atacctgatg cgccattttg cgaccgagtc aggcaaatcc aaagggcagt tttacacgcc
    27481 tgccgaagtc tcccgcatta tggcgaagat tatcggaatc agcgcagatt gccgtccagc
    27541 accagcgttt atgacccgac ctgcggctcg ggttcgctgt tgctcaaagc cgccgcccaa
    27601 gccggcagcc aaatcagcct ttacggtcag gaaaaagatg tggcaaccgc gtcccttgcc
    27661 cgtatgaata tgattttgca caacaacgaa accgccgaaa tcaacaccgg gaacaccttg
    27721 tccgattcgt ctttccgtga tgaaaacgac gggcttaaga ccttcgattt tgccgttgcc
    27781 aatccgcctt atcccgcccg aaaaaaacgg cgattacgcc tttttgctgc atctgctcaa
    27841 aagcctgaaa ccaagcggca aaggtgcgat tattcttccg cacggtgtgc tgtttcgcgg
    27901 caatgccgaa gcgcgtattc gcacggaatt gcttaacctt gaccttatta aaggcattat
    27961 cgggctgcct gccaacctgt tttacggcac gggcattcct gcctgcatca tcgtcatcga
    28021 caaagaacac gcccaaaccg cccaatttgc cgaagaggga acaaaccaag ttatcagcgg
    28081 cggcagcgtg tttatgattg acgcatcgcg cggcttcatt aaagacggca acaaaaaccg
    28141 tctgcgtgag caagacattc acaaaatcat cgacactttc acaaacctcg ttacagccgt
    28201 atggtgcatt taagcgaaat cgcagcacaa gattacaacc ttaatctgcc tcgctatatc
    28261 gacagcggag aagtcgaaga cctgcaaaat ctcgccgcct atctatcttt atggcggcat
    28321 acctgcgcac gatatggacg cattggaagc ctattggcaa gttttaggcc gtatgaaaaa
    28381 cgagttgttt gccgaacacg atggccactt taccactata caacggaatc gattgcaaat
    28441 ctttcccact ctcaacagct taaaatcctg cgggattggt gtggaattta gggctaatct
    28501 agtacagccc caaatttaat ccactataaa atcgaaagca gccaaatcaa agcccatata
    28561 ttggcgcacc ccgattacgc cgccttcaaa gccggacacc tagcaaagtt tgccgcgtgg
    28621 cacactcaaa acgaccttgc cgccatccaa ccgggcaggc ttatccggaa atggagcgaa
    28681 agcctgctgg acgcgttcaa acccggcagc ctgattgaag aatacgattt ctaccaaatc
    28741 ctgacggact actgggcgga aaccctgcaa gacgatgttt atctcatcgc ccaagatggc
    28801 tggaaggcgg ttaaaaacct ggccgaaatc accaaagaaa gcgatgaagc cgcgaacctg
    28861 accgtcgtct ttgaggaaac cgaaaccgac aaaaaaggca aagccaaaac caagcgcatc
    28921 agcaaaaaat accgcagcga agtcatcgcc cccgagctgg ttgcccgccg ctacttttca
    28981 gacggcatcg ccaagctgga agaaaaacaa agcgagctgg aacgcctaag ccaagaattg
    29041 gaaaaccaca tagaagaaca cggcggcgaa gagggtgcgc tgaacgacgt attggatgca
    29101 aaaggcaaac tttccgccaa acttctgaaa acctcattgg aagaaagcgg catagaagaa
    29161 ggcgaacggg ctgttttaca aaccacccaa acactgatga cgcaggaaaa agccgcgaaa
    29221 gacgcagtca aaacccaaat cgaagccctg aaccttgccg tattcaaaca atttggccga
    29281 ctttccgaag ccgaaatcaa gcagcttgcc gttcaagaca aatggcttgc cgatttacaa
    29341 agccgaatcg aaaatcgctt ggaaaacagt attcagcagc ttatcagccg cttgaacacg
    29401 ctggaagacc gctaccgcag cccgatggcc gagcttgccc gagaagtgga aaagtggcaa
    29461 agcaaagtca atgcccacct tgaaaatatg ggttttggag gctgaaatgg cagcacagac
    29521 aggctataag gcgagcgggt tttgagacct ttgcaaaatt ccccaaaatc ccctaaattc
    29581 ccaccaagac atttagggga tcgcggttcg ggtgtccgca ccgcttaata cgtcgtcgtc
    29641 cacgaactga cccatttgct cgaacgccat cgcaacgccc gttttatggc gcatatggac
    29701 aactttctcc caaactggca aagcatcaaa caacagctta atgccttgga gttatttgca
    29761 caaatatata atttaacata atatacatta tgcgaactat cggaaacagt tgtacgtgtc
    29821 cctgtggtct ttccaagtag gaaaattaaa gtatggccaa tgcggctgaa tgtatagccc
    29881 ggagcatccg cgtatccgaa ggtactgaac gacatgccct gcatcccttt cgacgcttcc
    29941 atccagccgg tattgggaaa tacgttgacc ttcaatataa tatacaggca gccgacacac
    30001 aggatatgcc gctgcttttt catcatttct tctgtcaaat ccttggaacg gtcgatttga
    30061 aaacacggct ttacatacgc cccagttccc tttgcagggc ttgaatattt tgctgcctgt
    30121 ccaatacatt gctttgtaat gcatttattt cttgatggtt gatgttgccg ccctttgcca
    30181 gacgtgcttg tgataacatt ttttgggctt caaccaatgc tttgcgttcg ttgctcaatt
    30241 ctgtttcgag aatggagcgt ctgctgttgt ttgagggtgc ttgttgcggc ggcggcgtat
    30301 tggattttgc cggcttggat actgttttga ccggggcttt atatttgaca acctgtccgc
    30361 cgtttgacgg tgatgatacc ggttcgggcg tttggggcgg gatatagcgt tcgctgctgt
    30421 agttgccgat tgggggcaaa tcggttgagt ggcagctttt ggacggcttg gtggtgtaaa
    30481 cggtttctcc gttgattgtg caggtgtaga ttttggccgc attcgcaccc aatgggcttg
    30541 aaatcaggga aaagttgatt aggattaaga ggagttttga tttcataatg tgcttgattt
    30601 tcggataatc atttgatttt ttggtatttt ttgtaatgct ttcttggcgt tttacgggct
    30661 tttcttttcg gttggttgct tttgattttc tttcggggct ttggcagcct gcttgttctt
    30721 tttcccggca ggttttttgt ttttcttttt actttctatg tgtattttgg cttcttaact
    30781 gagtttttta attttcaggc ggtatccgcc tccctgatgg ctgctgattt taggtaaatc
    30841 cgcatcggcg cacaatcctg ctggggctga tgtatatacg cttttgccat ttgagttgca
    30901 atggtatacg gaggcttgcg ccgccgcgcc ggaaagggac aggagaccca aggcggcaaa
    30961 aagtctgatg tttataccgg taaaaggcgg tgataacgcc cgaattatac cgttattggc
    31021 aggcaaagat aagcaccctg cccgcgcttc tttatcgctc ggcaaactgt ttctcgattt
    31081 cagtttcaat ctgttccaat tcttcttggc aagccagcca aacctcttcg atttgggcaa
    31141 gttgtgtttt gacttttgcc agctcggata aggtgtcctg caatttttct ttgttttcct
    31201 cgaagtaagc ttcttcttgt gctaaaaatg cttcacatgc cgtctgaatt tcggaaagct
    31261 gcgccatttc tttttcggca cggtctattt tctgctgtat cggcttgccg cgtcgggctt
    31321 tttcctgacg gatttgcgct tcgatgcgct tggtgtcttt gcggctttgg ctttgtgcgg
    31381 atgctgcggg cgcgacggcg gcgttttcct gtgccaaacg ccattggcgg tagtcgttca
    31441 aatcgccgtc gaagttcttc agacggcctt tatcgatcag gaggaagctg tcggtcgtgg
    31501 cttcaagcag gctgcgatcg tgcgatacga cgattaaggc gccttggaaa ctttgcagag
    31561 cgagcgtcaa ggcgtggcgc atatccaaat ccaaatggtt ggtcggctcg tcaagcagca
    31621 gcaggttcgg cttttgccag ataatcatgg caagagcgag tcgggctttt tctccgccgg
    31681 aaaatggttc ggttttctgc aacgccatat cgccgacaaa attgaagcct ccgaggaaat
    31741 ttcggatttc ttgttcgcgt acttcgggag aaagctgctg aatatgccaa acagggtttt
    31801 ggtcggagcg gatggtatcg agttggtgtt gggcaaaata gccgatattg agtttttcgg
    31861 aacggacgat gctgccggag agtaaatcga ttgtgcctgc caaagctttg ataaaggtag
    31921 atttaccgct gccgttgaca cccaataaac catagcgcgc gccgctttcc agcgacaggg
    31981 taatgtcgtg caaaacagtt ttgccttcgt aacccaaatc tgcgtgttct agctttaaca
    32041 aaggattggg cagatggtcg ggatggtaaa actcaaagga aaactcgctg tccagatgcg
    32101 cgggagcgat gcgttcgagc ttcgccaaag ccttcatgcg gctttgcgct tgaacggctt
    32161 tggtggcttt ggctttgaag cggtcgataa aggattgcaa atgtttgatt tgcgcctgct
    32221 gtttgacata ggcagcttgt tgttgcgcga gacgctgcgc acgttcgttt tggtaaaaat
    32281 cgtaattgcc gccgtattgc gtgagttttt gctgcgataa ttcaatggtt tgggtagttt
    32341 ccgcgttgag aaaatcgcgg tcatgggaaa tgatgatttg cgtgcagggt aaagaagcaa
    32401 ggtggttttc cagccacaag acggtttcca aatccaagtg gttggtcggt tcgtcaagca
    32461 agagcaaatc ggcgcggcaa atcagggctt gcgcaagatt caggcgcata cgccagccgc
    32521 cggaaaagga tttgacgggg cggctgtgtt cttcttgcga aaaacccagc ccgttcaaca
    32581 attttgccgc acgcgccggc gcggtataag cgtcgatttc ttccaattta gcatgatatt
    32641 ccgcctgctt catgccgtca ttttgcgctt ctgcctgcct caatgccgtc tgaaaagcct
    32701 gcaactcggc atcgccctgc aaaacgtaat ccaaagcgga aatatccaaa tcgggcgttt
    32761 cttgggaaac ggaagcgagc cgccagtttt tcggaatcga gacatcgccg ccgtcctgag
    32821 tgatttcacc cttgattaag gcaaacaggc tcgatttgcc cgttccgttt ttgccgatca
    32881 aaccgacgcg ctgaccggga ttgacggtag cgttggcttt gtcgagcagg actttcaaac
    32941 cgcgttgcag ggtgaggttt ttgatttcaa tcataacgga aacatcgtcg ggcgggaaaa
    33001 gcccgtattt tacctgaaag tcagtgccga tgccgtctga aacgggaaat ttacggctga
    33061 agccaagccc aagccctgcg cccttccgag tgcaggaaaa ccaatgtcct gaatgccgaa
    33121 tcggtattca tgcattccac gctgattccg attcgggaaa aatccgccat gattttggga
    33181 tggataaact cctgagccgc gcccgtcccg ataatcaata tttccggata gtcaacaggt
    33241 ttgacgtcgg acaacaggtt ttccggagtc agatcggaca aggttcggca ttgcgacagg
    33301 cagaccgaat ccttatgtac aagcacgggt ttatggaaac tttgccccgc cagccggatt
    33361 ccgcccgcac cgcattcata ttccgcaaac tgtccgtcta tcggattttc ttcaaacaac
    33421 atttttttac cccgttgccg catcatctac accgaaaggg atgcaaaatc agacaaattc
    33481 atgtaggatt ggcagatttc atctgacccg cctgccgatt tcagacggca tttgattcaa
    33541 agtgcggcac aattatatcg gcagcggata ttttcgtctt tcaatattta catttcagtc
    33601 ggcttacaag gagacacaat gaagccagta aacatcggtc ttttaggttt gggtacggtc
    33661 ggcggcggta cggctgccgt gttgcgggac aacgcggagg aaatttcccg tcgcttgggg
    33721 cgcgaaatcc gtatttctgc cgtgtgcgat ttgagtgaag aaaaagcccg acaaacctgc
    33781 ccgtccgcag cctttgtcaa agatccgttc gaactggtcg cacgtgaaga cgtcgatgtc
    33841 gtcgtcgaat tgttcggcgg taccggcatt gccaaagatg cggtgttgaa agccattgaa
    33901 aacggcaaac acatcgttac cgccaacaaa aaactgctcg ccgaatacgg caacgaaatc
    33961 ttcccgctgg cggaaaaaca aaacgtcatc gtccaatttg aagcggcagt agcgggcggt
    34021 atcccaatca tcaaagccct gcgcgaaggt ttggcggcaa acaggattaa atccatcgcc
    34081 ggcattatta acggcaccag caacttcatc ctctccgaaa tgcgcgaaaa aggcagcgcg
    34141 tttgccgatg tactgaaaga agcgcaggca ttgggttatg ccgaagccga tccgaccttc
    34201 gacatcgaag gcaacgatgc gggccataaa atcaccatca tgagcgcact ggcattcggc
    34261 acgccgatga acttttccgc ctgctacctc gaaggcatca gcaaactcga cagccgcgac
    34321 atcaaatacg ccgaagaact tggctatcgc atcaaactgt tgggcattac ccgcaaaacc
    34381 ggcaaaggca tcgagctgcg cgtccaccct accctgattc ccgaaagccg cctcttggca
    34441 aacgtcaacg gcgtgatgaa cgccgtgcgc gtcaacgccg atatggttgg cgaaacctta
    34501 tattacggcg cgggcgcggg cgcattgccg accgcttccg ccgtggttgc cgatatcatc
    34561 gacatcgccc gcctggttga agccgatacc gcccaccgcg taccgcatct ggcgttccaa
    34621 cccgcgcaag tccaagcgca aaccatcctg cctatggacg aaattaccag cagctactac
    34681 ctgcgcgtcc aagctaaaga cgaaccgggc acgctggggc aaatcgccgc gctgttggca
    34741 caagaaaacg tgtccatcga agcactgatt caaaaaggcg tgattgatca gaccactgcc
    34801 gaaatcgtga ttctgaccca cagcacggtc gaaaaacaca tcaagtcggc aatcgcagcc
    34861 atcgaagcac tggattgcgt ggaaaaaccg attaccatga tccgcatgga aagcctgcat
    34921 gactgagccg aaacacgaaa tgctgacgaa agagcaggtt gccgcgcgca aaaaagcaaa
    34981 agccaaaatc cgcaccatcc gcatttgggc gtgggtcatt ttggcgttgc tcgctttaac
    35041 cgccctgctc tcccaatgcg cgatgtccaa accgcaggca aaacagaaaa ttgtcgagtc
    35101 ttgcgtgaag aatattccgt ttgccgaaaa atggcaaaac gatttgcggg cccgcggttt
    35161 agattcaaac aatacccgcc tcgccgtcga ctactgcaaa tgtatgtggg agcagccttt
    35221 ggacagattg agcgagaaac agattagatc cttcggcaaa ctcggcgcac aagaacagct
    35281 tgacctgctc ggcggcgcaa atgcctttga agcacgtgac aagcagtgtg ttgccgattt
    35341 gaaatcagaa taatgtggac cgataaaaaa gccgattctt taaagaatcg gcttttttca
    35401 taaaaaacgg cttacagtgc gtctttcaaa gctttgccgg cgcggaattt aggcgttttg
    35461 gcggcggcaa tggtcagagg ctcgccggtt ttggggttgc ggccttggcg ttccgcacgt
    35521 tcgcccacgt agaaagtacc gaaaccgacc aaagtaacgg tgtcgccttg tttcagggcg
    35581 gtggttactg cattggtagt ggcatccaaa gctttttgtg cggcggcttt ggaaatgtcg
    35641 gcttcttgag caatcgcttc gatcaattca gacttgttca caatcagtcc cttcctgtct
    35701 taaaaaatga tgaaatgccc gaatactcgg ggtttgtact gcttgagcaa ctttcgcttt
    35761 atagcaattc tgaaattgcc gtgtcaagca aaaaatacgg aatcacccta tttgacaggc
    35821 tttcaggacg aaaccgcatt tttacaacac atttcctgcg tttcaatgtt tggttgccct
    35881 gctgcggggt tttggttttg aagcggattc cgccgccgct tccgcaccag aaggttctgc
    35941 ccaaggctca ggctggcttt ccaaacccag agccaatacc tcgtctatcc atttgaccgg
    36001 atggatggtc aggccggttt tcacgttttc agggatttct tccaagtctt tgacgttgtc
    36061 tttcggaatc aggacgtgtt tgatgccgcc gcgcaaggcg gccaacagtt tttccttcaa
    36121 accgccgatg ggcaaaactt cgccgcgcag ggtaatttcg cccgtcatcg ccacatcggc
    36181 gcgtaccggg attttggtaa aggcagatac cgccgccaag gtcatcgcaa tacccgcact
    36241 agggccgtct ttcggcgtcg cgccttcggg aacgtggatg tggatgtctt ttttctcgta
    36301 aaaatcagga gccaaaccca ctgattccgc acgggagcgg acaaccgacc acgctgcgga
    36361 cacggattcc ttcatcacat cgcccaactg gccggtgcac tgaatcacgc ccttacccgg
    36421 caatgctgcg gcttcgacgg tcagcaattc gccgccgact tccgtccacg ccaaaccggt
    36481 aacctgcccg atacggtttt cgctttcggc aacgccgtaa tcgaagcggc gcacacccaa
    36541 atagtcgtgc agatttttct catttacttt aaccgcttta ggtttggctt tgctggtttt
    36601 cttggtttca gacaacctct tcttatcttc gtccaaggta atctgcatca ccaccttgcg
    36661 gcagattttg gcaatttcgc ggtcgagcga acgcacgccc gcctctcggg tgtaataacg
    36721 gataatatcg cgcaccgcgc tttcttcgat tgccaattcc ccttctttta caccgttgcg
    36781 cttcatttgc ttcggtacga ggtactgcat cgcgatattg attttttcgt cttcggtata
    36841 gccggacaga cggatgattt ccatacggtc gagcaacgga gtcggaatat tcagactatt
    36901 ggatgtggcg ataaacatca catcactcaa atcgtaatcc acttccgcat aatgatcggc
    36961 aaacttgttg ttttgttcgg gatcgagcac ttcgagcaac gcgctggcgg gatcgcctcg
    37021 gaagtcgtta cccaatttgt cgatttcgtc gagcaggaac aaggggtttt tcacgccggc
    37081 ttttgccata ttctgcaaaa tcttaccggg catagagccg atataggtgc ggcggtgtcc
    37141 cctgatttcg ctttcgtcgc gcacgccgcc caaagccatg cggacatatt tccgccccgt
    37201 tgctttggcg atggattcgc ccaaagaggt tttgcccacg cccggagggc cgaccaggca
    37261 cagaatcggg cctttgagtt tgtccatacg tttttggacg gcgaggtatt ccaaaatccg
    37321 ttctttgact ttttccaggc cgtagtggtc ggcatccagc accagtccgg ctttggcgat
    37381 gtctttgctg acgcgggatt ttttcttcca cggcagctcg agcaaagtgt cgatgtagtt
    37441 gcgtacgacg gtggattccg cagacatcgg tggcatcatt ttgagctttt tcagttcgga
    37501 caggcatttt tcttccgctt ctttggtcat acccgccttt ttgatatctg cttccaaggc
    37561 atccagttcg ccgttttcgt cttcttcgcc cagttctttg tgtatcgctt taatctgttc
    37621 gttcagataa tattcgcgct gggatttttc catttggcgt ttgacgcgtc cgcgtatgcg
    37681 tttttcggcc tgcataatgt cgagttcgga ttccagctgt gccagcagga attccatccg
    37741 tttgccgatt tcgggaattt ccaaaatctg ttggcgttgc gccagtttca actgcaaatg
    37801 cgctgcgacc gtatcggtta gccggctgtt ttcggcaatg ccgttgatgc tgccgataat
    37861 ttcggcgggg atttttttat tgagtttggc gtattgttca aactgcgcca acagggtgcg
    37921 gcgcacggct tcgaggtcgg tattgccgcc cgtgtcttct tccacgaccg tctctatatg
    37981 ggaaacgaac agaccgcccg tgtcttcaat ggtcagaaca cgtccgcgat acagcccttc
    38041 gaccaatact tttaccgtgc cgtcgggtag tttcaacact tgcaggactt gtgcgaccgt
    38101 accggtctga tacaggtcgg cggcaatcgg ttcttctacc gccgcatcgg tttgcgccaa
    38161 caggaaaacc ggctcctcgc gggtaatggc gttttccagt gcggcgatgg atttcggtct
    38221 gccgacaaac agcggcagaa ccatatgcgg gtaaacgacg acatcccgca aaggaagggt
    38281 tgccaaggcg gcatattcct caaaatgctt ttctttttgt gtcataggta ctctcttgtg
    38341 tctgacagat tgccgatttt cgcgtacatt ggggttgaag gtattatttc aagcatatgt
    38401 ggtttattta tggagtttga tgcgatgccg tctgaaacat tccggcttca gacggcatgg
    38461 gcttggaaag acaaggcggg aacaaaaaac tgttctgtgt tgccgctcct tgctgtacca
    38521 tccgtatggt ttgcggttct gccgccctat tttcaaaaca tgacgcaggt atcccatgtc
    38581 ttcttttatt tttcccgatg acgggttcga atggcgcaac gaaagtctgc aaaagccgcc
    38641 caaaggttgg cattatgtcc gcgaaagcgg tgcagacggc attttgaagg ctgcctatca
    38701 aaatattgca actgtctagc agggcgattt ccacaatgcc aaacaggtgc tttctgcaat
    38761 gaagaagagg gttcgcaaac ctgccgccgt ccgttccgat gcggatatcg ccgccctttt
    38821 ccatgcccac cgtatgaagc aggcgcagca gagccgtatt ctgaatatgc ttgccgttga
    38881 aatccgcccc ggttttgtgt tggacaacaa acgcgcgccc gatatacgct ccgctttgct
    38941 cgacgtgtac ggagaggcgg acggcaaacc gtttttcctg ccgctcaatc tgctgctggg
    39001 gtttatgggt gcgcacgagt ggcataagaa aggggttgcc gttccgcagc tgggcggcag
    39061 catacacgtt cctttcggcg tattctcgcc gttgcgcggc gaatacctcg acctgctcgc
    39121 ccatgcgccg tcaacgggtt ttcagacggc attcgatatc gggacaggct ccggcgtgct
    39181 tgccgccatt ttggcgaaac agggcattcc ttccgtcatc ggcacggata ccaatccgaa
    39241 agccgtcgcc tgcgcccgtg ccaatattgc ccgtttgggc tttgaaaaac aggttgagat
    39301 acgggaaacc gatctgtttc ccgaagggtt tgccgatctg attgtctgca atccgccctg
    39361 gcttcccgcc aagccgactt ccgccgtcga atccgcgtta tacgaccccg aatctgcgat
    39421 gctggctgcg tttttgcggg atgcgccgaa acatctgaat cccgacggag aaatccgcct
    39481 gatcatttcc gatcttgccg aacatctgca cctgcgtcca tccgattttc tggataaggc
    39541 atttgctcag gcgggtttgc gtgttgccga tatgatgaaa accaagccga agcacaaaaa
    39601 agccgcgaat ccgagcgatc cgcttgcttt tgcgcgaacc cgggaaacca ctttcctata
    39661 ccgtttgaaa aaggcataag gggcggcggc gcggcattcg ggcggattat tcttgtgaaa
    39721 atacccgctc gagcatactg cccaatgccg tctgacgcgt tttgacggtg gcggcagctt
    39781 gccggaaggc ttcttccccg ccgaagaata cgaacttctc tctcgcgcgg gtaatggcgg
    39841 tatataacag ctccttactc aatccggaca atgcatcgtc cccttcgtcc gaaggtgcgg
    39901 cggaaggcgg cagcagccat acttcccggt attccgaacc ttggcttttg tggacggtca
    39961 tggcgaatgc gggttcaaat tcgggcaggc agcttaccgc taccttttta aatccgtccg
    40021 catcggcaaa ataggcggca aggctgccct gccgtccgac atcttccata atcagtccga
    40081 tgtcgccgtt gaacagttca agcgcgtagt cgttctgcct gatcataatc ggctctccgg
    40141 caaaatatgc caaatgttcc ggtatgttca ttttgcggcg tacatggcgg caataggctt
    40201 cgttgaagtc ttccgcatcc tgccgccaag ctgccagaac cacgatatcc gaaatgcccg
    40261 cgtatgcggc ttcgatattg ccgtctttta ccgcctgcca ataggctttg tgtgcccggt
    40321 acaacctttc gactcgagcg ttcggactgc attccgaatg ttccagttcg tccggaaacc
    40381 ggtcaaacaa tgcccacgcc ccttcatcgc ccgatacggc ggcacgggca aggcagccga
    40441 tgccgctgtt gtcgccgaag cggtggctga acgacagatg ggcggtgttt tgcgccaaca
    40501 cgggtggatt tgcgctgacg ctgaaaccgt gttccggaag gaagccggcc agcctttggt
    40561 gcgtttctcc gtccaaaacg gttttttgtg acaaaacgga cagcaccgcc cctattccga
    40621 cggacgggag ctggttttca tcccccagca gaatcacgcg cgcgccggtt ttgaccgctt
    40681 ttaaaagttg cagcatcaat gccgtatcca gcatagaggc ttcatcgata accaatacgt
    40741 caaacggcag cgggtaaaca gggttgaacg ccgcctgcat tttgggcggg cgcagcttca
    40801 gcagtcggtg gacggtttgc ccttccagtt tgagcaaatg gcggcggacg gcctccggcg
    40861 cgtcaaaacc gttgattgca cggtgcagcg cgcgcgccat atgtgccgcc gctttgcccg
    40921 tcggtgcggc aagcgcgatg ttgggaagat tttcgtcttc accgcaaatc agcgccagca
    40981 gtttggcaac cgttgtcgtt ttgcccgttc ccggcccgcc ggtaatcacc ataaaagact
    41041 gcaacagtgc caaggcggcg gcatcgcgct gcccttcgct gcccgtgcct tgaaaccatt
    41101 ttgcgaggtt ttgcctcgcg cctgccgcgt cgggggcgga tgtgccggct gccgccaagc
    41161 gttttatctc ggcagccaaa tcgtattcca actgccacat cctgcccaaa aacagccttc
    41221 tgccttccaa aatcaaaggc gcggcggatg ttccgacaac gggtgcgagt gccgacagcg
    41281 cgtcagcctc gccaccgctc aaacggataa acgaatgacc gttttgcaat gcctgaaaca
    41341 ggcgttcggt gcagtttgca agcacttcgt cgccggaacc cgcatagtgt tccaaaaaac
    41401 ggattgccgc ccttgccgcc gcttgggcaa attcatctgt ctgccgttcc attttattcc
    41461 ttatccaaat gccgtctgaa ggcgtggggc ttcagacggc gcggtgttgt ttcggctgtt
    41521 taggcgtttg cgccctgttt ggggtgcagg ctgccgtctt tcatgaccat cacgcgctcg
    41581 aagcggccgg cgagttcgtc gtcgtgcgtt acgaccacca gccccgttcc caattctgtt
    41641 ttcagttcca gcatcatatc caaaacattc ctggcgttcg cacgatcgag attgccggtc
    41701 ggttcgtcgg caagcaggca tttgggttgg gtaaccaagg cgcgtgcaat ggcggcacgc
    41761 tgccgctcac cgcctgaaag ttcgcccgcg cggtgcgtcg aacggtgttt cagtccgacc
    41821 ttttccagca tcgccattgc cgcctccgca gcctcttcac ggcttttttt gccgatcaga
    41881 agcggcatca tcacattttc cagtgccgaa aattcaggca gaagatggtg gaactggtac
    41941 acgaaaccga gatggcggtt acgtaaatcg cccaaacgcc gctggtttaa ggtacgcaaa
    42001 tcctcgccca tcagcagcac cctgccttca gacggcatat ccagcccgcc caaaatatgc
    42061 agcagcgtcg atttgccgct gcccgaagaa ccgatgatgc cggtgctttc ccctgcgtgg
    42121 atttccaaat ccaagccgtg cagcacccga acgtccaaac cgccgtcccg atagcgtttg
    42181 cccacgcctt cgcatttcaa aatcaactca ctcataacgc aaagcctccg ccggttgggt
    42241 ttttgacgcg cgccggctcg ggtagagcgt ggcaacgaaa gacagtccca aagaaatgca
    42301 ggcaatcagg gcaacgtcgc ccatatcgac atcgctgggc aggtagtcga taaaataaac
    42361 ctgcgaattg atgaggtgga caccgagcag gttttcaaaa aacgccacga ccctgccgac
    42421 gttccaaccc aaaagcacgc cgcagaccac acccgccagc gtgccgaaaa agcctgaaaa
    42481 cgcgccctgc accataaaaa tcttcatcac gccagcaggg gaaagaccca aagtccgcaa
    42541 aatcgcaatg tccgcctgct tttccgtaac cgccatcacc agggaagaga caaggttgaa
    42601 cgccgccaca gcgataatca gcgtcaggat gatgaacatc atccgttttt ccagttcgac
    42661 cgcttcaaaa tagctgcggt tgctgtacgt ccaatcgcgc acccaaaccg cgtccctttg
    42721 cgcctccgga atcagtgttg ccgtcaaggc gggagcgttt tgcggatcgg cgagcttcag
    42781 ccgcagcccc gcaacttcct tatccaaacg gtacagcacg cgcgcgtctt ggatatgcgt
    42841 cattgccaat gagttgtcca cttcgtaaac gcccgtctta accagaccga ccacggtaaa
    42901 ctgtttcaac ctcggtacga ctccggcggg cgtaacattg ccctccggcg tgatgacggt
    42961 aactttattg ccgacttccg cccccaaagc ctccgccaag ccgacaccga ggataatgtc
    43021 aaactcgccc ggaatcagat cttcaaattt gcctgccggc attttgtcgc cgtattccac
    43081 cactttgcgt tcttcagacg gcaaaatgcc gcgcatctgc acgcccctga tttcgcccgc
    43141 attggccagc aatgcctgat tggaaacata gggcgcggca gccaaaatac ctttgcggtt
    43201 ttcggtaaac cgaagcaggt tgcgccaatc cgtatccgta ttatcgatat agccgatttc
    43261 ggcgtgcggc gcgacattca ggagctgccc gcgtatttct ttctgaaagc cgttcataac
    43321 cgacaagacg acaatcagcg cggttacgcc caaggcgatt ccggcaatcg aaaccatcgt
    43381 gataaacgac ataaagccgt tgcgcttttt cgccctgaga tacctcaagc ctatccaagc
    43441 ctctagagaa aacataacgc taccttaaaa atgtctgcaa acgtgccgcc ccggacggcg
    43501 gtttgggggc ggcggcaaaa gtttattgta ccgtaaaacc caggcagcgt ccgaacgccc
    43561 gagtgcgggg gacggggcgg cagagcgggc ggaaaacttg cacaacgcgt caaactgccc
    43621 tatcctttcc ttcagaaaaa ccgtttcttg aggaaaacaa tgaatatccg aactgctttt
    43681 gctttgtgcg ccatcgcctt atccgccgct gcggctgcct acgccaaaga aatcaaaatc
    43741 gatgccaaca acacgcctta ttccgaagcc gacgcgcaaa agctggcggc aacggcagtc
    43801 ggtatgggtg ttaaggaacc tatcagcctg aacggcggca gcggcagcat taccgtgtcc
    43861 ggcagcagcg cgacgcagtg cgtgttcaaa gtcggcaacg gaggcgcatt gcagattcaa
    43921 gggctaaact gcaagtaaac cgcccggaaa aatgccgtct gaaggcttca gacggcattt
    43981 tgcattggcg gcgttatgcc ccgccttctt taatcaggcg gcgttcgtac accgcctgcg
    44041 ccagcgttcc cgcatcgaca tattccaatt cgccgcccaa gggaatgccc tgcgacagcc
    44101 tgctgacttt gtaaggcagg tttttaaaaa actcggacag gacatacgcc gtcgcattgc
    44161 cttctgcggt aaaagcggtt gcaataatga tttcttcgac ttccccgccg cccagccgtt
    44221 gcgccagcct gtccaatgcg atggcggata cgtccattcc caatgccgta ttgatttgcc
    44281 ccatcaggac gaaatacagc ccgtcgtggc agtttgccgc ttccatattc gacacgtcgg
    44341 caggcatatg caccaccatc agccgccgcc cgtcgcgtgt ttcatcggca caaatatcgc
    44401 acaatccgcc ttcgcaaaac gtgttgcaca tcgcgcaatg gtaaacctgc ttcaatgccg
    44461 tctgcaaggc atccaccagt ttttcagcct ctttgcgctt gtgttggagc aaatgatacg
    44521 ctatccgctg tgccgatttc ggcccgacgt tgggtaaaac cttcagcgcg tcgatcaatc
    44581 cttggaaggc atcttgtttt ttgtggctca tcatattccg ccgtatggga aaacggccgg
    44641 aatattccga ccgttatttt gtcaacaaaa gtgtcaatta ctgaccgtcg ccgttgtcga
    44701 ccgattgcgc tcctttggtc tgtttgattt tgccgttgaa ataacgtatc aacaagtcga
    44761 aagtattggc agactgctgt tgcgccaaag cctgttttgc aagcggaagc tgtgcggcga
    44821 tatcatccgg cggggttaca gcctgtactt cgacaatcac gggtgccggc agaccgatca
    44881 gcctgacgta ggcgggtttg ccgtttgccg gttttgcttt cagcagttcc gcataagcct
    44941 cgggcggcat ggactgcctt gcctgctgtg cgcccaaaac ggacacttcc gaccatttca
    45001 cgtcaacagc cttgccgccg ttcagttggg taagcacgtc ttttgccttg ttttcggcaa
    45061 gtttggcggc ttcggtacgg atataagcct gacgtaccgc gtctttggct tcggcaaacg
    45121 gcagggtttt ctcttcgcgg acttctttgg cgcggacgac ccacgcggtt tcgctgttga
    45181 tggtcagcac ttcggaattg tgttttttct tcaatacgtc gtcgctgaat acggcattga
    45241 tcaggttttc gggcataccg gacatttgcg cgtcctgcct actcagccaa gtttcttggg
    45301 tttcgacttt caaaccgctg tttttggcgg cttcggcaag cgaggaagga tggttgaacg
    45361 catcgtcgcc caatttttct tttgccttgt tgaagtcggc aaccgccttt ttcattttca
    45421 attcgttttc gacggcggct ttttcctgct cgaaagaagg tttggcttca tttgccggca
    45481 aacgcgccac gcgctcttca aatgcatttt tcacttccgt ttcactgacg gtctgcttgt
    45541 ctgcaaaatc cttcagattc aaggcgacat attccaattt gaccgcctgc ggcagcagat
    45601 agtctttttt gttcgcatta taaaatttct gcaaatcggc ttcagacact ttgacttggg
    45661 cgatgaactc gtcggggttg aaagtgtgcg aacggatggt gcggttgacc tgtgtcagcc
    45721 tgatcagctg ttccgcctgc gcgtcgccga ccaatacgcc gttttggacg aggtttacca
    45781 aattctgcaa ggcaaactga tcgcggattt cttcgacaaa ctggtcttca gacatatggc
    45841 gttgggaaag gtagcggttt aaaagcgcgt ggtcgaattt gccgtttgcg tcgtggaaat
    45901 tgggatcgtc cacgataatt tgcttgattt gttcggaaga aaccgaaatg cccatcagct
    45961 tcgcgccctg tttcaggtag gcgcgttgca gcagggattg gaacaccgcg tcgcgcgaag
    46021 ggccgccgcc gtccgcctgt tcgttctgta tggcgttgtt gatggagtgg tcgctgattt
    46081 tttcgtcgcc cacttggacg atgtagtcgg cacccggatg ggataccgtg ctgaccccga
    46141 agccgacgaa ggttaatgca atcaggccca aaaggacttg ggcgggcgtt ctgtattttt
    46201 cgatggaatg gaacatattt taaatcggga tatagaatgg gaacgggaaa ttcaagtcgg
    46261 gtattgtaac ggtttttatc cctgtctgca cggggcttgc cggttgaaga tgccgtcgta
    46321 ggtttcttcg ctgaagccgg cgtaaacctt gccgccgcac tccaatacgg gacgcttgat
    46381 caggctcggc atttcggaca tcagtttgac ggcctccgcc gtcgaggaca gcactttttg
    46441 ctgtgtttcg gcatcgagtt tgcgccagct tgtcccgcgt ttgttgagca gggttgccaa
    46501 aggcacttgt tccagccacg agcagatttc cgcttcagac ggcctctgtt ttttaaaatc
    46561 ccgaaattca aactccaagc cgtatccggc aagccggttt ttggcttttt tgaccgtatc
    46621 gcaatttggg atgccgtgaa ggactatcat ttggaaacct tttgtctgaa ataataaaac
    46681 ggatatttta ctataagtgt ctgaaaattt gcccgtctgt ttcagacggc ggggcggtta
    46741 tgttacaatc cgaaaattcg aaaaatttaa tctcttgttc aataaaggct ttaccaatca
    46801 tgatttctac caacggcatc accatgcagt tcggcgcaaa gccgctgttt gaaaacgtat
    46861 ccgtcaaatt cggcgaaggc aaccgttacg gcttgatcgg cgccaacggc tcaggcaaat
    46921 ccaccttcat gaaaatcctc ggcggcgatt tggaacagac ggccggcgaa gtggcgattg
    46981 aaaacggcgt gcgtttgggt aaattgcgcc aagaccagtt tgcctacgaa gacatgcgcg
    47041 tgctggacgt ggtgatgatg gggcataccg aaatgtgggc ggcgatgacc gaacgtgatg
    47101 cgatttacgc caatcccgaa gccaccgaag acgactacat gaaagccgcc gaactggaag
    47161 ccaagttcgc cgaatacgac ggctacaccg ccgaagcgcg tgccgccgaa ctgttgagcg
    47221 gcgtgggcat ttccgaagat ttgcacaatg cgaaaatggc ggaagtcgcc ccgggcttca
    47281 aactgcgcgt attgctggcg caggcgctgt tctccaagcc ggatgtattg ctcttggacg
    47341 aaccgaccaa taacttggac attaatacca tccgctggtt ggaaggcgtg ttgaaccaat
    47401 acgactccac gatgattatc atcagccacg accgccactt tttgaacgaa gtctgcacgc
    47461 atatggcgga tttggactac aacaccatca ccatctatcc gggcaactac gacgactaca
    47521 tgctcgcctc cgcccaatcg cgcgaacgcg ccctgaaaga caatgccaaa gccaaagaga
    47581 aactgcaaga gctgcaagag ttcgtcgccc gcttctctgc caacaaatcc aaagcccgtc
    47641 aggcaaccag ccgtctgaaa caggccgaca aaatcaaatc ggagatggtc gaagtcaaac
    47701 cttccacccg tcaaaacccg tatatccgtt ttgaagccga tgaaaaagcc aagctgcacc
    47761 gtcaggctgt ggaagttgaa aaactggcga aacgctttga aacccagttg tttaaaaacc
    47821 tgaacttcat ccttgaagcg ggacaacgcc tcgccatcat cggcccgaac ggcgcgggca
    47881 aatccaccct gctgaaactc ttggccggcg cgtacaaccc cgaatattca gacggcctgt
    47941 tgccggacga aggcaccatc aaatgggcgg aaaaagccag tgtcggctac tatccgcaag
    48001 accatgaaaa cgacttcgac gtcgatatgg acctgagcga atggatgcgc caatgggggc
    48061 aggaaggcga cgacgaacaa gtcatccgcg gcactttggg gcgtttgctc ttcggcagta
    48121 acgatgtcgt gaaaaaagtg aaggttctct ccggtggtga aaaaggccgt atgctttacg
    48181 gcaaactgtt gctgttgaaa cccaatgtct tagtcatgga cgaaccgacc aaccatatgg
    48241 acatggaaag catcgaatcc ttgaacatgg cactggaaaa atacaacggc acgctgattt
    48301 ttgtctccca cgaccgtcag ttcgtttcct ccttggcaac ccaaatcatc gaactggacg
    48361 gcaaaggcgg atatgaacac tacttgggcg attacgaaag ttacttggag aaaaaaggcg
    48421 tagcataacc gccggttgga acaatgccgt ctgaagccgc ttcagacggc attgttgata
    48481 actttaaaat aggaagcata tgcagactta tctcgtcggc ggtgccgtcc gcgattatct
    48541 tttgggcttg cccgtcaaag accgcgattg ggtggtcgtc ggcgcagacg cacaaaccat
    48601 gctggcgcaa ggcttccagc cggtcggcaa agattttccc gtgtttctcc atcccgaaac
    48661 acacgaagaa tacgccctcg cccgcaccga gcgcaaaacc gccaaaggtt acgtcggttt
    48721 cagtttccac gccgacaaag acgttacgct ggagcaggat ttgatgcgcc gcgacctgac
    48781 catcaacgcg atggcgcaag atgcggacgg caagattatc gaccctttcg gcggacaacg
    48841 ggatttggcg gcaggcattt tgcgccacgt ttccccagcc tttgccgaag accccgtccg
    48901 catcctgcgt actgcccgct ttgccgcgcg ttacaagttt gaaatcgccg aagaaaccat
    48961 aaagctgatg cggcagatgg tggaaaacgg cgaagcggac gcattggttg ccgaacgcgt
    49021 ctggcaggag tttgcgaaag gtttgatgga aaaaaatccg cgcaaaatga ttgaagtgtt
    49081 gcgcgaatgc ggcgcgctca aagtcttgct gcccgaagtc aatgccctct tcggcgtgcc
    49141 gcaacgcgcc gactaccatc ccgaaatcga cagcggcatc cataccctga tgacgctgca
    49201 acgcgccgcc gatatgggct tgagcctgcc cgaacgctat gccgccctgc tgcacgactt
    49261 gggcaaagcc aaaacaccgt ccgacatcct gccgcgccac cacggacacg acctcgccgg
    49321 tgtcgaaccc gtgcgcgaag tcaatcagcg gctgcgtgcg ccgaaacatt gcgccgagct
    49381 tgccgaattg gtttgccgtt ggcacattat tttccaccaa gtcggacagc ttaaaagcca
    49441 aaccattctg aacgttttga aaaaaaccga cgctttcaga cgacccgaac gctttcagac
    49501 ggcattgaac gtctgcattg ccgacacgca aggccgtctg aaccgcgaac acacgcccta
    49561 cccgcaacgc gcgcactggc tcgccttact cgaagccgcc aatcaggcgg attcgggcaa
    49621 aatcgccgcc gaatgccgcg cacagggaaa agcgcacctt atcgccgaac aaatcgaccg
    49681 ggcgcggctg gcacaaatcg ccccattgca aaaagcgttt cgagcggcgc aagacaaaac
    49741 agaaaaacat taaaacgtcc aatgcagcca
//
LOCUS       DAWIVB010000002        39316 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWIVB010000002 DAWIVB010000000
VERSION     DAWIVB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.002"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(52..717)
                     /locus_tag="WIX60_000047"
     CDS             complement(52..717)
                     /locus_tag="WIX60_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ5304382.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFIVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(827..1735)
                     /locus_tag="WIX60_000048"
     CDS             complement(827..1735)
                     /locus_tag="WIX60_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ5304383.1"
                     /translation="MQPPKASDIISHLQAGGQQRFFRRHLPAIQYTHLNNYLNIKEKQ
                     RMSAIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGTQSTVARLTMTVYLPAEQKG
                     THMSRFVALMEQHTEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIR
                     SLLDYDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADA
                     EVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATALIADKR
                     IKSFVVESENFESIHNHSAYAYIAYP"
     gene            1803..2960
                     /locus_tag="WIX60_000049"
     CDS             1803..2960
                     /locus_tag="WIX60_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ5304384.1"
                     /translation="MKFATQAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFVFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPMDCWLVLRGIKTLALRMEAHCQNALEIARRLEAHPAIEKVFY
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     ANHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(3023..4024)
                     /gene="hemB"
                     /locus_tag="WIX60_000050"
     CDS             complement(3023..4024)
                     /gene="hemB"
                     /locus_tag="WIX60_000050"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ5304385.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GAAREEDVPSMPSVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKEEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(4207..4491)
                     /gene="yhbY"
                     /locus_tag="WIX60_000051"
     CDS             complement(4207..4491)
                     /gene="yhbY"
                     /locus_tag="WIX60_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ5304386.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            4601..5221
                     /locus_tag="WIX60_000052"
     CDS             4601..5221
                     /locus_tag="WIX60_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ5304387.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGSSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            5285..7252
                     /gene="ftsH"
                     /locus_tag="WIX60_000053"
     CDS             5285..7252
                     /gene="ftsH"
                     /locus_tag="WIX60_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ5304388.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            7473..7904
                     /locus_tag="WIX60_000054"
     CDS             7473..7904
                     /locus_tag="WIX60_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ5304389.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            7897..8175
                     /locus_tag="WIX60_000055"
     CDS             7897..8175
                     /locus_tag="WIX60_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ5304390.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            8228..8806
                     /gene="pth"
                     /locus_tag="WIX60_000056"
     CDS             8228..8806
                     /gene="pth"
                     /locus_tag="WIX60_000056"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ5304391.1"
                     /translation="MSNTIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGRNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            8867..9073
                     /locus_tag="WIX60_000057"
     CDS             8867..9073
                     /locus_tag="WIX60_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ5304392.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSVEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            9070..9432
                     /locus_tag="WIX60_000058"
     CDS             9070..9432
                     /locus_tag="WIX60_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ5304393.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            9663..11078
                     /locus_tag="WIX60_000059"
     CDS             9663..11078
                     /locus_tag="WIX60_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ5304394.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            11197..11706
                     /locus_tag="WIX60_000060"
     CDS             11197..11706
                     /locus_tag="WIX60_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ5304395.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            11869..13251
                     /locus_tag="WIX60_000061"
     CDS             11869..13251
                     /locus_tag="WIX60_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ5304396.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPK
                     NLQDLIVELKNGEAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(13271..14026)
                     /locus_tag="WIX60_000062"
     CDS             complement(13271..14026)
                     /locus_tag="WIX60_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ5304397.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(14036..14752)
                     /locus_tag="WIX60_000063"
     CDS             complement(14036..14752)
                     /locus_tag="WIX60_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ5304398.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(14742..15569)
                     /locus_tag="WIX60_000064"
     CDS             complement(14742..15569)
                     /locus_tag="WIX60_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5304399.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVVRNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            15854..16762
                     /locus_tag="WIX60_000065"
     CDS             15854..16762
                     /locus_tag="WIX60_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ5304400.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            16842..20456
                     /gene="recB"
                     /locus_tag="WIX60_000066"
     CDS             16842..20456
                     /gene="recB"
                     /locus_tag="WIX60_000066"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ5304401.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHADT
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYDAQQLHDFNQNESEILHWAESARTALDNWNKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            20495..20854
                     /locus_tag="WIX60_000067"
     CDS             20495..20854
                     /locus_tag="WIX60_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ5304402.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            21096..21575
                     /locus_tag="WIX60_000068"
     CDS             21096..21575
                     /locus_tag="WIX60_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ5304403.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(21851..23230)
                     /gene="radA"
                     /locus_tag="WIX60_000069"
     CDS             complement(21851..23230)
                     /gene="radA"
                     /locus_tag="WIX60_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ5304404.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(23416..24480)
                     /locus_tag="WIX60_000070"
     CDS             complement(23416..24480)
                     /locus_tag="WIX60_000070"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ5304405.1"
                     /translation="MISLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(24634..25074)
                     /locus_tag="WIX60_000071"
     CDS             complement(24634..25074)
                     /locus_tag="WIX60_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304406.1"
                     /translation="MGSSDSFKEKKEIFEIGTPAYRQKLIDVWKKSINGNEKSWVLFE
                     NGTCVILLEPEKDLAKQAKEMLSKWGKVQIGTPSADFGIITLDSGDGYAVSCHHPEIF
                     TLILKEEGLDEDFKIGIEGRSHRDCDAEEPKVIHIEDKRTIETP"
     gene            complement(25107..26330)
                     /locus_tag="WIX60_000072"
     CDS             complement(25107..26330)
                     /locus_tag="WIX60_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ5304407.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDEIGEPQKAEAQRNLVLE
                     AVSDDERHAALEQHS"
     gene            complement(26327..27661)
                     /locus_tag="WIX60_000073"
     CDS             complement(26327..27661)
                     /locus_tag="WIX60_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ5304408.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NEKTASEPAAASDVKTIEAPSLPSERKPEQPAKKQTVPEKAGRSPSAKGERA"
     gene            complement(27676..28437)
                     /locus_tag="WIX60_000074"
     CDS             complement(27676..28437)
                     /locus_tag="WIX60_000074"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ5304409.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(28458..28877)
                     /locus_tag="WIX60_000075"
     CDS             complement(28458..28877)
                     /locus_tag="WIX60_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304410.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            complement(29128..29448)
                     /locus_tag="WIX60_000076"
     CDS             complement(29128..29448)
                     /locus_tag="WIX60_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ5304411.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETWLLKP
                     LSAQ"
     gene            complement(29471..30097)
                     /gene="upp"
                     /locus_tag="WIX60_000077"
     CDS             complement(29471..30097)
                     /gene="upp"
                     /locus_tag="WIX60_000077"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ5304412.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLATELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(30203..30514)
                     /gene="grxD"
                     /locus_tag="WIX60_000078"
     CDS             complement(30203..30514)
                     /gene="grxD"
                     /locus_tag="WIX60_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ5304413.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEVGELQELLK
                     A"
     gene            complement(30615..30830)
                     /locus_tag="WIX60_000079"
     CDS             complement(30615..30830)
                     /locus_tag="WIX60_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ5304414.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREAKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQY"
     gene            complement(30856..31635)
                     /locus_tag="WIX60_000080"
     CDS             complement(30856..31635)
                     /locus_tag="WIX60_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ5304415.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(31640..31990)
                     /locus_tag="WIX60_000081"
     CDS             complement(31640..31990)
                     /locus_tag="WIX60_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ5304416.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(31994..32971)
                     /locus_tag="WIX60_000082"
     CDS             complement(31994..32971)
                     /locus_tag="WIX60_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ5304417.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(33005..34117)
                     /locus_tag="WIX60_000083"
     CDS             complement(33005..34117)
                     /locus_tag="WIX60_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ5304418.1"
                     /translation="MTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRT
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            34303..35004
                     /locus_tag="WIX60_000084"
     CDS             34303..35004
                     /locus_tag="WIX60_000084"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ5304419.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYAWGQVPQLPARFASDGILIEAAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            35148..36941
                     /gene="lepA"
                     /locus_tag="WIX60_000085"
     CDS             35148..36941
                     /gene="lepA"
                     /locus_tag="WIX60_000085"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ5304420.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVIYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            37084..38103
                     /gene="lepB"
                     /locus_tag="WIX60_000086"
     CDS             37084..38103
                     /gene="lepB"
                     /locus_tag="WIX60_000086"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ5304421.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRS
                     GTAIR"
     gene            complement(38259..39164)
                     /locus_tag="WIX60_000087"
     CDS             complement(38259..39164)
                     /locus_tag="WIX60_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ5304422.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKRQSSH"
BASE COUNT         9417 a        11175 c         9936 g         8788 t
ORIGIN      
        1 agcattaaaa aatgccgtct gaaaatcctt gcgttttcag acggcattgc cttatgccca
       61 aataacggtt tcttccaagg gcctgcgcgc cttcgtggcg atttcctgaa cgcggtaacc
      121 gaatgtcgcg gcgacggaca cgccccattc tgccgcatcg aacaaaccga actgcccgga
      181 caatacgcgt tccatatcgg catagttgaa accttccacc gggcaggaat cgatacctgc
      241 catcgccgca cccgtcatca tgttgcccaa cgcgatatag gtctggcggc aacaccagtc
      301 aaacaaggcg cgcgaatcgt ccaaaatctt gatgtcgtcg gcttgaaacg cctgatacct
      361 cgccaaagat tttgccatgg catccggttc ggtaacgccg cgccgtttga ggctttccaa
      421 cataaacggg ctgtcgaagc gggcattttt ctttgccaaa aacaccacca aatgactggc
      481 ggtatccaaa gcatccgcca taccccaaga aaacggcttg attgcctgtc ggatttcagg
      541 gttttgaacc acaataaact gccaaggctc cgaaccgacc gaactgggcg acaaacgccc
      601 gagttctaaa ataaactgaa aatcctcggc actgattttg cgtgccgcat cgtaatgccg
      661 gcatgattta cggtttttaa atgcggatag aacctgctct ttgcttaata ctgtcatcgt
      721 gcatcctttt attacttgta ttgcaaattg atttccgata cggcggcaga ttcgccaata
      781 acgcccaaac gcctttcaga cggcatttgg ttcatcgcaa acacgcctac ggatacgcga
      841 tataggcata agccgaatgg ttgtggatag attcgaaatt ctcactctcg acgacaaagc
      901 tcttgatacg cttgtcggca atcagtgcag tagcgacatc acgcaccata tcttccacga
      961 atttcggatt ttcgtaggcc ttttcggtaa cgtatttttc atcggggcgt ttgagcaggc
     1021 cgtagagttg gcagctcgcc tgcgtttcca cataatcgat aacttcctcg ataccgactt
     1081 cggcatcggc agtcaggctg acggtaacgt gcgaacgctg gttgtgcgcg ccatattggg
     1141 aaatttcttt ggaacacggg caaagcgagg ttacgggaat catgaccttc atactgtggc
     1201 cgtatgcccc gtctttgatt tcgcccgtga gacagacatc ataatccagt aaggaccgga
     1261 taccggaaac cggcgcggtt ttcttgcgga aaaacggaaa agaaacgctg attttgccgg
     1321 cgcgggaatc caaaagcgcg accatttcgg cggtcagcct atgcaattgt gcaaaatcca
     1381 aagcttcggt atgttgctcc atcaatgcga caaagcgcga catatgcgta cccttctgct
     1441 cggcgggcag gtagaccgtc atggtcaggc gggcaaccgt ggattgcgtt ccttcggcgg
     1501 tttgcagggt aatcgggaag cgcaggtctt taatgccgac ctgattgatc ggcaggttgc
     1561 gtaaatctct gctggattgc acgtctgcaa tggcactcat gcgttgtttt tccttaatat
     1621 tcagataatt attgagatgt gtgtattgta tggcaggcag atgccgtctg aaaaaacgct
     1681 gttgaccgcc tgcctgcaaa tgcgagatta tatcacttgc ttttggcggc tgcattgatt
     1741 gtttctattt tcgggatagt gataaaatcc cgacttattc atatcttaca aggcagaaac
     1801 agatgaaatt cgccacccaa gccatccatt ccggctacga ttgcgacgaa cacaaccgtg
     1861 cgctgatgcc gccgatttat caaaacagta tgtttgcgtt gcacgagatt ggcgaaaatg
     1921 tgccttaccg ttattcgcgc ctgagcaacc cgacccgtca gattttggaa gacaccgttg
     1981 ccgatttgga acacggtgcg gcaggttttg tgttttccag cggtatggcg ggaattgatg
     2041 ccgtgtggcg taccttcctg cgccctggcg atactattgt cgccgtcgct gatatttacg
     2101 gcggcgcgta tgacttattg gtcgatgttt atcaaaaatg gggtgtaaac gttgtttttg
     2161 ccgatttggg caatccggat aatctggacg agctacttaa agcgcacaag gtcaaactgg
     2221 tttggctgga aacgccgtcc aatccgcttt tacgcttggt agacatcaaa gcccttgccg
     2281 caaaagccaa agcggccggt gcgctggtgg gtatcgacaa cacctttgcc acgccgtatc
     2341 tgcaacagcc cttggatatg ggttgcgatt ttgtgttcca ctctgctacc aaatatttgt
     2401 gcggccattc cgacgtattg atgggcatcg tcgttgccaa aaccaaagaa ctggcgcagc
     2461 ctttgcacga tatgatggtg cataccggcg cggttgccgg cccgatggac tgctggctgg
     2521 tgttgcgcgg catcaaaacg ctggctctgc gtatggaggc acactgccaa aacgcactcg
     2581 aaatcgcgcg ccgtctggaa gcccatcctg ccatagaaaa agtgttctat cccggcctgc
     2641 cgtctcacga acattacgca cttgctcaag cgcaaatgcc caaaggcata ggcggcgtgg
     2701 tgacggttta tctcaaaaac gacacgcgcg aagcggcaaa cagcgtgatt aaaaacatga
     2761 aactggtcaa aatggcttcc agcctcggcg gcgtggaaag tttggccaac cattgctatt
     2821 cccagtccca cagcggcgtg ccgcataatg tgaaaatgga aatgggcatc agggtcggac
     2881 tcctgcgttt ctccatcggc attgaagacg tagatgatat ttggaacgat atttccgccg
     2941 cactcgatac gactttgtaa accgtaaaaa tgccgtctga aaccatggtt tcagacggca
     3001 tttcaatatt gcgaatcaaa tatcaacgct tcagcatctt tgccgcctca atggcgtaat
     3061 aggtcaaaat cccgtccgca cccgcacgtt tgaatgccag caggctttcc aaaaccactt
     3121 tgccgccgtc cagccagccg ttggcaaccg ccgcctgcaa catcgcgtat tcacccgaaa
     3181 cctgataggc ataagtcggc acgccgaact cttctttcac acggcggaca acgtccaaat
     3241 acggcaaacc gggcttgacc atcaccatat ccgcaccttc ctgaatatcc aacgctactt
     3301 cgtgcaacgc ctcatcggta tttgccggat ccatctgata ggtcttttta tccgccttgc
     3361 ccaaattgcc cgaactgcct accgcatcac ggaaagggcc gtagaatgca gaagcatatt
     3421 tggcggaata tgccataatc cgcgtatgga tatgcccggc atcctccaaa gcctcgcgaa
     3481 tcgcaccgat acgcccgtcc atcatatcgg aaggggcaac gacctgcgcg cccgcttcag
     3541 cgtggcacaa agcctgcttg accaaaacct cgacggtttc atcgttcatc acataaccgt
     3601 tttcgtccgt cagcccgtcc tgaccgtgaa ccgtataagg atcgagcgca acatccgtca
     3661 taatgcccag ttcgggaaat ctctcgcgca aggcgcggac ggctgccggc acgagtcctt
     3721 cgggattgta cgcctcctcc gcacgctcgg ttttgtttgc cgtaaccacg ggaaagagtg
     3781 ccaacatcgg aataccgagc ttcaccgcct cttccgccgt aaacagcagc ctgtccaaac
     3841 tttgacgctt cacactcggc atagaaggca catcctcctc acgagccgcc ccctccaata
     3901 cgaacaccgg ataaatcaaa tcatcggcag tcagcgtatg ctcgcgcatc agacgccgcg
     3961 agaagtcgtc cctgcgcata cggcgcatac gcgaagccgg aacattgcgg taaggaaact
     4021 gcataatccc tccaatcatc ctgtcaacaa ttcaaaccat acggaagccg ccgcaacttg
     4081 cagacagcat cgggcggcaa tcaaaatccg ttttcaccgg aaagggtttc ggcccgccga
     4141 aatgccgcgc cggaggacag cgtcccgccc attaaagaac aatagcaaaa caacagcttc
     4201 aggctgtcag ccttcgatat tcttacgcca caataccaaa agtttcccga tatgctgaac
     4261 aggttgcgcg tcaaccgcct cacataaggc agtgcagatt tcgatacgtt cggcacggtc
     4321 gtcgccgaat acgcgcactt taatcagctc atgcgccgtc aatgccgcat cggtttcctt
     4381 gatgaccgcg tccgtcagac cctgctgacc gaccatcaca acaggatgga gatggtgcgc
     4441 gcgcgctttc agttccaaaa tttctttggt gttcaatttg gtatccgtca ttttcctact
     4501 gcttgaataa aaagaataat gcggcattgt acgcgatttg gcacgacatc ggcaaaatta
     4561 acgtacaata cgcggtttcc attccgaccc gcgaaccact atggctgtac gttccaaatc
     4621 ctcaaaagcg tggctgcacg aacacgtcaa cgaccactac gtccatatgg cgcaaaaaga
     4681 cggctaccgc gcccgtgccg catacaaact tttggaaatc aacgacaaag acaaattaat
     4741 caaacccggc acggtacttg ccgacttggg cagcgcgccg ggaagctggt cgcaggttgc
     4801 cgccaagctg acgggttctt ccggagcagt tttcgccttg gacatcctgc ctatggaagc
     4861 catagggggc gtctccttca ttcagggcga cttccgcgag aacgacgtac tggcacaatt
     4921 cgaaaccttg ttggacaacc gcccgctcga ccttgtaatt tgcgatatgg cgcccaatat
     4981 gtcgggaaac gccgtaagcg atcaggcacg cagcttttat ctgtgcgaac tggctttaga
     5041 cttcgcctcg caacacctga aaaccggcgg cagctttttg gtcaaagtct ttcagggtgc
     5101 aggctatcag gaatacatgg cagccatgcg cgaaattttc ggcacggtgc agacgcgcaa
     5161 acccgaagcc tcgcgcaatc gctccagtga gatttattta ttgggcaaaa ataaacgctg
     5221 acaatacaga cggcgtgctt tacaatcatt tccgttttac acctcaatta tggagccttg
     5281 ctaagtgggg aacaccttta aatcaatcct tgtctgggtc gccttgggta tcggcctgat
     5341 ggctgcgttc aacgctttag acggtaaaaa agaagacaac gggcaaatcg aatattctca
     5401 gttcatccaa caggtcaaca acggcgaagt atccggcgtc aacatcgaag gatccgtcgt
     5461 cagcggctac ctgattaagg gcgagcgcac cgacaaaagc accttcttca ccaacgcgcc
     5521 tttggacgac aacctgatta aaacactgct cgacaaaaac gtccgtgtaa aagtaacgcc
     5581 ggaagaaaaa ccgagcgcgc tggctgccct gttttacagc ctgctgcccg tcctgctgct
     5641 gattggcgcg tggttctact ttatgcgtat gcagacgggc ggcggcggca aaggcggcgc
     5701 attctcattc ggcaaaagcc gcgcccgcct actggacaaa gatgccaaca aagtgacctt
     5761 tgccgatgtc gccggctgcg acgaagccaa agaagaagtg caggaaatcg tcgattacct
     5821 caaagcgccg aaccgctatc aaagcctcgg cggtcgcgtg ccgcgcggca tcctgctggc
     5881 gggcagcccg ggtacgggta agacgctttt ggcaaaagcc attgcaggcg aagccggcgt
     5941 gccgttcttc agcatttccg gttccgactt tgtcgaaatg ttcgtcggtg tcggtgcgag
     6001 ccgcgtccgc gatatgttcg agcaggcgaa gaaaaacgct ccctgcatca tctttattga
     6061 tgaaatcgac gcagtcggcc gccaacgcgg cgcaggtttg ggcggcggca atgatgagcg
     6121 cgagcaaaca ttaaaccaat tgttggttga aatggacggt tttgagagca atcagactgt
     6181 aattgtgatt gcggcaacca accgccccga cgtactcgat cctgcgctgc aacgccccgg
     6241 ccgtttcgac cgccaagtgg ttgtccccct gccggacatc cgggggcgcg aacagatttt
     6301 gaacgtccat tctaaaaaag tgcctttgga caaatctgtg gatttattgt ccctcgcgcg
     6361 cggcacgccg ggtttttccg gcgcggattt ggcgaacttg gtcaacgaag ccgccctgtt
     6421 tgccggccgc cgcaataaag tcaaagtcga tcagagcgat tttgaagacg ccaaagacaa
     6481 aatctatatg ggtccggaac gccgcagtat ggtgatgcac gaagacgaaa aacgtgcgac
     6541 ggcgtatcac gaatccggac acgcgattgt tgccgaaagc ctgcccttta ccgaccccgt
     6601 ccacaaagta accattatgc cgcgcggacg tgcgctgggt ctgacttggc agcttccgga
     6661 gcgcgaccgc atcagtatgt ataaagatca gatgttgagc cagctctcca tcctgttcgg
     6721 cggacggatt gccgaagaca tcttcgtcgg acgcatctcc accggcgcat caaacgactt
     6781 tgaacgcgca acccaaatgg cgcgcgaaat ggtaacgcgc tacggcatga gcgacaaaat
     6841 gggcgtgatg gtttatgcgg aaaacgaagg cgaagtcttc ttaggacgca gcgtaacccg
     6901 ttcgcaaaac atttccgaga aaacccagca ggacatcgat gcggaaatcc gccggatttt
     6961 ggacgagcag tatcaggttg cctacaaaat cctcgatgaa aaccgcgaca agatggaaac
     7021 catgtgcaaa gccctgatgg aatgggaaac catcgaccgc gatcaggtac tggaaatcat
     7081 ggcgggcaaa caacccagtc cgcccaagga ttacagccac aacctgcgcg agaatgcgga
     7141 cgcggcggaa gataacgcgc cgcacgctcc gactcgggaa gaaaccgaag cacctgcccc
     7201 ggcagacacc gcttcgacag agtccgagca gcagcctgaa aacaaggctt aacttcccga
     7261 acaaacggca gcccgcaagc tgccgttttc ccatcctgtt ttcagacggc attttccgct
     7321 caatgccgtc tgaaacgcca aagccgtatc ggtttttgcg gatctgcgat atggcggttt
     7381 aaattgaaat cagacgaggc aaggctgtac cggtttaaaa ttaaaccgct ataataccgc
     7441 ctttcgaata cacgcccgca ttggtgatgc ctgtgaaaaa agttgaaaaa aacatcttgg
     7501 tgctgcacgg cgcggacaaa atgttcgagc tggtcgataa ggttgaagac tatccgcact
     7561 ttctgccgtg gtacagcaag accgaggtca tcgggcgtag cggcaacgaa ctgaaggcgc
     7621 ggctgtttat ggactatatg cacgttcgcc aatcattcgc cacgcacaac cgcaacattc
     7681 cgggcaggga aatccgtatg gaactgctcg agggtccgtt caaaacctta cgtggaacgt
     7741 ggaaatttat cgatttgggc gacgatatgt gcaaaatcga attcaatttg gaatacgatt
     7801 tttccaatgc cgttttgtcc gccttaattt cccccgtctt caaccacctt tccgccacgc
     7861 tggtcgaagc gttcgtcaaa gaggcagacc gccgttatgc ttgaaattga gattgtgtac
     7921 gggctgcccg accgacaggt tttgaaaacc atgcagcttg ccgagggaac aaccgtccgc
     7981 gccgccgcac tgcaaagcgg tttggacggc atatttgaag atttaaacct gcattccgcg
     8041 cctttgggca ttttcggcaa agccgtcaaa gacgacacgc ccctgcgcga cggcgaccgc
     8101 atcgaagtgt accgcccgct gttgatcgac cccaaagaag cgcgccgcaa acgcgttcaa
     8161 aatcaagaag aataaccatg ccgtctgaag ccttcagaca gcattcgaca gaaacacgga
     8221 aaatatcatg tcaaacacaa tcaaaatgct tgtcggattg ggcaaccccg gcaaagaata
     8281 cgaacaaacc cgacacaatg cgggtttttg gttcctcgac gaactggcgt ggaaatggaa
     8341 ggcttcattt aaagaagaaa aaaaattctt cggcgaagtc gcccgtgccg ccctgcccga
     8401 cggcgacgtt tggctgctca aacctgccac gttcatgaac cgttccggac aggcagttgc
     8461 cgcgcttgca cagttctaca aaatcaaacc cgaagaaatc ctcgtcgtcc acgacgaact
     8521 cgacattccc tgcggacgga ttaaattcaa actcggcgga cgcaacggcg gacacaacgg
     8581 cttgaaagac attcaggcaa aactcggcac ggcagactat taccgcctgc gcctcggtat
     8641 cgaccacccg ggcgaccgca acctcgtcgt cggctatgtc ctgaacaaac ccagtacgga
     8701 acaccgccga cagattgacg atgccgtcgc caaatccctg caagccatac ccgacatcct
     8761 tgccggcaaa tgggaagaag caacccgctt cctgcacagc aaatgacccg atgccgtctg
     8821 aagccctttc agacggcatg ttcccgattt ccatatccga acagtcatga ccgaactcaa
     8881 gcagcttatc caaaccgaat ccatccccgt catcgaagaa accctcgatt tcctgctgta
     8941 cgaatgcagc atcgacgacg caccgtccgt cgaagaagtg gcacaatggc gcgacatact
     9001 tgccgcacgc ggcggcaaat tcctgcgcct gtccaaaatc tgccaaacgt ggctggacga
     9061 ggaggcggca tgaagctgcc gcgcaaccgc ttcagcctgc tttccgcatt gtggtttgcc
     9121 ggcggcatct attcgctgct cttcaaagct gccgacaccg cgccgccgcc gtttccgcat
     9181 ttcgacaaag cagcacacct tgccctgttt ttcgcacaaa tctggctttt gaccaaagca
     9241 ttcaaaaccg gaaaacttcc catcccctac cgcagcctga tggtctttgc cctctgtttc
     9301 gccctcttca gcgaatgcgc gcaggcatgg tttaccgcaa cgagaaccgg cagtttgggc
     9361 gatgttcttg ccgatatggc aggtacggtt ctcgcactct ttgccgcccg cgccgccgac
     9421 cgcccggact gaatcggttt attttcccca aacaggatgc acctcttccc gatacatcct
     9481 atttttcccc ttacatatca atcccatctc ataaacaaaa ccgacaaatc gtttacaata
     9541 tatttacact acatcagatt acaaatatac tcgacccagt tgcagaatcg gcggtttcac
     9601 aaaccgtttc ggattgcacg acacaaaaga aaaaccgatt ttattgcctt aaaggagtat
     9661 tcatgaacct gcatgcaaag gacaaaaccc agcatcccga aaacgtcgag ctgctcagtg
     9721 cgcagaagcc gattaccgac tttaagggtc tgctgaccac cattatttcc gccgtcgtct
     9781 gtttcggcat ttaccacatc ctgccttaca gcccggatgc caataaaggt atcgcgctgc
     9841 tgattttcgt tgccgcactt tggtttaccg aagccgtcca cattaccgta accgcactga
     9901 tggtgccgat tctcgccgtc gtactcggtt tccccgacat ggacatcaaa aaggcgatgg
     9961 ctgatttttc caacccgatt atctacattt ttttcggcgg cttcgcgctt gccaccgccc
    10021 tgcatatgca gcggctggac cgtaaaatcg ccgtcagcct gttgcgcctg tcgcgtggca
    10081 atatgaaagt ggcggttttg atgttgttcc tcgttaccgc ctttctgtcc atgtggatca
    10141 gcaacaccgc caccgccgcg atgatgctgc ctctagcaat gggtatgctg agccacctcg
    10201 accaggaaaa agaacacaaa acctacgtct tcctcctgct cggcatcgcc tattgcgcca
    10261 gcatcggcgg cttgggcacg ctcgtcggct cgccgcccaa cctgattgcc gccaaagccc
    10321 taaatctgga cttcgtcggc tggatgaagc tcggcctgcc gatgatgctg ttgattctgc
    10381 ccttgatgct gctctccctg tacgtcatcc tcaaacctaa tttgaacgaa cgcgtggaaa
    10441 tcaaagccga atccatccct tggacgctgc accgcgtgat cgcgctgttg attttccttg
    10501 ccacagccgc cgcgtggata ttcagctcca aaatcaaaac cgccttcggc atttccaatc
    10561 ccgacaccgt tatcgccctg agtgccgccg tcgccgtcgt cgtcttcggc gtggcgcaat
    10621 ggaaggaagt cgcccgcaat accgactggg gcgtgttgat gctcttcggc ggcggcatca
    10681 gcctgagcac gctgttgaaa acatccggcg cgtccgaagc cttgggacag caggttgccg
    10741 ccaccttttc cggcgcgccc gcatttttgg tgatactcat cgtcgccgcc ttcattattt
    10801 ttctgaccga gttcaccagc aacaccgcct ccgccgcatt gcttgtaccg attttctccg
    10861 gcatcgctat gcagatgggg ctgcccgaac aagtcttggt attcgtcatc ggcatcggcg
    10921 catcttgtgc cttcatgctg ccggttgcca caccgcctaa cgcgattgtg ttcggcacgg
    10981 gcttaatcaa gcaacgcgaa atgatgaatg tcggcatact gctgaacatc ctctgcgtag
    11041 tattggttgc tctgtgggct tatgctgtac tgatgtaaac catcgaccta aacaacaaga
    11101 ccgtctgaaa gaatattttt cagacgacct tgaagtttgt cgttcagaca caatttgtcg
    11161 aatcattcaa aaccggattc taacgaaagg aaacccatga ttatcctgca caccaacaaa
    11221 ggcgacatca aaatcgaact cgatttcgac aaagcccccg ttaccgccaa aaacttcgag
    11281 caatacgtca aagacggctt ctacgacggc gtaatcttcc accgcgtcat caaaggcttc
    11341 atgattcaag gcggcggcat ggatgaaaac atgaacgaaa aagaaacccg cgatccgatt
    11401 caaaacgaag cgtccaacgg cctgcccaac gataaataca ccatcgccat ggcacgcact
    11461 tccgaccccc attccgccag cgcgcaattc ttcatcaaca ctgccgacaa cgctttcctg
    11521 aacttccgtt ctaaagagct gtacggcaaa accgtcgtcc aagactgggg ctatgcagta
    11581 ttcggtaaag tcgttgacgg ttttgacgtt gtcgatgcca tcgaaggcgt atccaccaaa
    11641 cgccacggct accatgacga cgtaccgagc gaacctgtcg tcatcattaa agccgaagcg
    11701 gtataaaccg gcaatccgaa agcagcctgc acaaaggctg ctttttttgt ttcaggcggc
    11761 ataaaatcca agcacatatg ccgtctgaac acctcccgca agccttcgga caatgttgca
    11821 acgtgttaaa atagcctcct ttcctgaaac cacaacaagg aaaccatcat ggcaaatatt
    11881 gcccgcgaca tcttcaaagc ctacgacatc cgaggcattg tcggcaaaac cctgactgac
    11941 gatgccgcct atctcatcgg cagggccatc gccgccaaag ccgccgaaaa aggcatgacc
    12001 cgcatcgcgc tcggacgcga cggacgcttg agcggtcccg aactgatgga gcacatccaa
    12061 cgcggcttta ccgacagcgg catcgacgtc ctcaacgtcg gtatggtcgc cacgcccatg
    12121 ctctattttg ccgctatcaa cgaatgcggc ggcagcggcg tgatgattac cggcagccac
    12181 aatccgcccg attacaatgg tttcaaaatg atgctcggcg gcgacacgct cgcaggcgaa
    12241 gccattcaag aacttttagc cattattgaa aaagacggtt ttgttgccgc cgacaaacaa
    12301 ggcaacgtaa ccgaaaaaga catctccggc gaataccaca accacatcgt cggccacatc
    12361 aaactcaaac gccccatgaa aatcgccatc gacgcaggca acggcgtagg cggcgcattc
    12421 gcaggcaaac tctacaaagg cttgggcaat gaagttaccg aacttttctg cgaagtggac
    12481 ggcaacttcc ctaatcacca ccccgaccct tccaaaccga aaaacctgca agacctcatc
    12541 gtcgagctca aaaacggcga ggccgaaatc ggcttagcat ttgacggcga tgccgaccgc
    12601 ttgggcgtgg ttaccaaaga cggcaacatc atctatccgg accgccaact gatgcttttc
    12661 gcgcaagacg tgttgagccg caatcctaaa gccaaagtga ttttcgacgt caaatccacc
    12721 cgtctgcttg cgccttggat taaagaacac ggcggcgaac ctgtgatgga aaaaaccggc
    12781 cacagcttta tcaaatccgc catgaaaaaa accggcgcgc cggttgccgg cgaaatgagc
    12841 ggacacatct tcttcaaaga acgctggttc ggcttcgacg acggtctgta cgccggcgca
    12901 cgcctcttgg aaatcctgtc tgcctccgat aatccgtccg aagtgttaaa caacctgccg
    12961 caaagcattt ccacgcccga actcaacatc gccctgcccg aaggcagcaa cggccatcag
    13021 gttatcgacg aactcgccgc caaagccgaa tttgaaggcg caaccgaaat catcaccatc
    13081 gacggtctgc gcgttgaatt tcccgacggc ttcggtctga tgcgtgcttc caataccacg
    13141 ccgattctgg tgttgcgttt tgaggcggat acgcaagaag ccatcgagcg gattcaaaac
    13201 caattcaaag ccgtcatcga aagcaatccg aatctaatct ggcctctgta aacacaggaa
    13261 aaatgcttaa tcaaatcttg gtagattgga tttggcttaa aaatctccgc gtccgttcgt
    13321 gtttggggtg gtcgaacaac tctttcgggc tgccctgctc tacgataacg ccgccgtcca
    13381 tcacgacaac ggtcgtggca acttccagcg cgaacttgat ttcgtgggta acgacgacca
    13441 tcgtccaacc ttcccgcgcc aattccttca tggcgttcaa cacgtcttgc accaactcgg
    13501 ggtcaagcgc ggaagtgggt tcgtcaaaca acatcagctc gggctgaatc gccaatgctc
    13561 gggcaatgcc gacgcgctgc tgctgaccgc cggaaagctg gtagggatac aaatccactt
    13621 tgtcgcccaa gccgactttt tccagcagtt tcagagcctc ttcgcgcgct tgggcggcag
    13681 gcttgccctg tacggcaacc ggtccttcca tcacgttttc caaggcggtt ttgtgcggaa
    13741 agaggttgta ttgttgaaac accatgcctg atttgcggcg cagtgccaaa atatcgtgtt
    13801 tgcttggttt tttagaaaaa tcgattttca gcggtcgctc gttgtcgaac tcgatttgtc
    13861 cgtcttcggg catttccaac gcgtttaggc agcgcagaaa cgtcgttttg cctgagccgg
    13921 aaggcccgag gatgacgacc acctgccctt tgcacacatc caaatcgatg ccgcgcaaaa
    13981 tagtgttttc gccaaaggtc ttatggatat tgcggatttt aatcatgaca actccttatt
    14041 tggcgacgta gcggtcgaaa cgtttttcca aacgcgcctg aatcaggaac agcactttac
    14101 aaaaacacca gtaaaccaaa gcggcttcga tatagacggg caaaaagtca taagtgcggt
    14161 ttgccgtttc ctgcgcgacg cggaataatt ccgttaccgt cacgactgcc gcgagcgagg
    14221 tgtttttaaa caaaccgata aactcgttgc tcaaaggcgg cacggcaacg cggaatgcct
    14281 gcggcgcgac aatgcggcgg aacgtctgca tataggtcat gccgatggag aaacctgctt
    14341 cccattggcc tttaggtacg gacaaaattg ccgcgcgtat ggtttcggaa gcgtatgcgc
    14401 cgacattgag cgaaaagccg atgatggcgg caggaatcgg gtcgatatag atgccgacgg
    14461 aaggcagccc gtaaaacaca atcacaagct gaaccaacag cggcgtaccg cgaatgacgg
    14521 aaatataaaa ttccaccaat ttcagcagga ttttccgcac gatgccgccg gcgggcataa
    14581 tccgcaccaa agccacggct accgcaatca tcataccgat aacgaaagaa gctgccgcca
    14641 aaggcagaga gaccgcgaag ccggctttga ccataggcaa aaacgcgctg acaatcatat
    14701 cggcgcgtgt ttccgtcata aacggcagcg aagcaaggaa attattgaac actgatgtct
    14761 tttccgaaga attgttcgcc cagttttttc agcgtaccgt cggctttcag ctcgttgatt
    14821 gccgtactga atttcgccac ggcttcgtca ttgcccttgt tgacaatcag gccggaaccg
    14881 actttttcat cggcaggtgc ggaccaaacg attttcacgc ccgcattcgg gtttttcttc
    14941 agatagtcca aaaccgccaa ttcgtcgttc agggttgcat cggcacgttt ttgttcaatc
    15001 agggtcagcg attgcgccaa accgtcaaca gccaccaaat ctgcgcctgc agctttggct
    15061 ttttcgccgt agttgctggt cagggattgt gcggttttca cgcctttgat gtcggctata
    15121 gatttgatgt tgctgtcgtt acggacaacc aatacggcac cgctccagct gtaaggatcg
    15181 gatttgtcga atgtcgcttg gcgttcgggg ctggtcagac cgacttggtt tgccaccacg
    15241 tcgaaacgcc ccgccttcaa acccgccatc atcgaatccc attgcgtttc tttaaactcg
    15301 actttcacgc ccagtttttc cgccacggcg cgggttactt ccacatcgta accggtcagt
    15361 ttgccgtcct tgtcgtggta ggtaaacggt gcgtaagtgc cttccgtgcc gacggtaacc
    15421 gtgcctttat tgttgatgcg ctcgattaaa gaaccggaaa ctgccgattg tgcaggcgcg
    15481 gaagatgctc cgctgccgcc ttccgaaccg ccgcaggccg ccaaaaccaa tgcggcaata
    15541 ccgccgagta cgaatttttt caacatcatc ctctcctgta aaaagtgtgc aagtgcggca
    15601 ttctaagtga aattgcggca aacacaaaga atactttttt tatatggtta ttccaaatcg
    15661 gaatggaaaa gatatgccga agccgccgga agcggaaata aagggggcgg gaaaggcggc
    15721 gggcggcacg aaaccgaaaa gcggcatcag gtatgtaaac ggcggattca cgcacgccgt
    15781 tccggcaaaa atccgcctgc cgattcggtg ctataatcta tccgaaacag atgacaccaa
    15841 aaaggaacat ctcatgaaca cgccccaatc cgccatcatt cccgaccacg cccaagccgg
    15901 catctttatc gaagccgact tcgccgccaa tcgcctcaac gatattaaag ccgcctgccg
    15961 cgcttcgctc gacgcgttaa ctgccttgaa agcccgcttt ccggacgata ttttgggtct
    16021 gaccatcgcc ttcggcagca aagcctgggc aacattcgga catacggacg aaggcagtga
    16081 aatcaaaccc ttctccgaaa tgggcaacgg gcttgcgccg tccacgcagc acgatatgtc
    16141 catccacatc caatccttcc gccaaaacgc cgcctacgcg cttgcccaat ccgttttggg
    16201 cgcgttcggc gacagcatat gcgtcgcgtc cgaagaacac ggtttgcgcc tgtatcagga
    16261 tcgcgggctg gacggtttcg tcgacggcac ggaaaacccg cagggcgatg aaaatgtccg
    16321 cgaagtcgcc atcatccccg aaggaaaacc cgatgcgggc ggcagctatg tcctgctgca
    16381 aaaatacctg cacgatttga aaaaatggga tgccgtcccc gtcgccgaac aggaggcctc
    16441 ggtcggacgc ggcaaggaaa ccgacgacga attcggccgc gatgtccgcc tgcccgattc
    16501 gcacctcggc cgcgtcaacc tgaaagaaaa cggagtcggc ctgaaaatcg tccgccgcag
    16561 cctgcccttc ggcaaaatca gcggcgaaca cggtttgatg tttaccgcat actgccgcac
    16621 gctgcacaat atcgaagcgc aattattgag tatgttcggc gatacggacg gcaaaaccga
    16681 cctgctgctc cgacatctct ccgccgccgt ttcgggcgga tactactacg cgccgtctgt
    16741 cgaacgcctg caaaacctct aagccggacg atgccgtctg aaaccatttt tcagacggca
    16801 ttttcccgtt ttaccgtcca accgttttcc gagcgcgcac tatgtctgca agcatccaag
    16861 cattcgaccc gctaaccgtc cccatttccg gcaccaacct gattgaggcc tctgccggca
    16921 ccggcaaaac ctacggcatt gccgccctgt ttacacgcct gatcgtatta gaacaaaaaa
    16981 acgtcgaacg cgtattggtc gtaaccttca ccaaagccgc caccgccgag ctgaaaacac
    17041 gcctgcgcgg gcgtttggac gatgtgttac aagttttaga aagcaaagga attgccaaac
    17101 ttggagacga cacgctttca gacggcattg ccgcctactg cgccgaacac cacgaaggcg
    17161 acaccttcct gcccgaactc ttaaaacagg ctttgcaaaa agagggccgg acgcgtctga
    17221 ttgtccgcct caaggccgcc atcgggcaat tcgacaacgc cgccatctat accatccacg
    17281 gcttctgcca gcgcatcctg cgcgactacg ccttcctgtg ccaagcaccg ttcgatgtcg
    17341 aaatgaccga agaagacggc gaccgcctgc ttatcccggc gcaagatttt tggcgggaac
    17401 gcgtcagcaa tgatccggtg cttgccgcat tggcgcttaa acgcaaagct gtgccgcaaa
    17461 ccgtccttgc ccaaatttcg cgctatcttt cccgcccgta cctgaatttc cgccgtccgc
    17521 aggcggattt gaaacaggcg cagcgcaacg ccgaaacctc atggcaaacc atctgccgcc
    17581 tgctgccaga actggaagcc ggcttttggc gcattcatcc cgacctcaac ggcaacagtt
    17641 accgcaaaaa cagcttcggc aaccttttta aagaactggc acaaaaatcc gccgccggac
    17701 aactgccctt tctggacaaa gacacacacg acagactctt gaaacttgca tccgacaaac
    17761 tcgaagccgg actgaaaaaa ggcaaaacgc ccgatgcggc agtatttgcc gaattgcaga
    17821 aactggcaga cttcgggcgc gatttgaacg cactcgaaga agcggaagaa gcaacaatga
    17881 tccggctgca actggattta atcgaatacc tcaaccgcag ccttgccgag atgaaaaaat
    17941 cgcgccgcga acgcggtttc gacgacctgc tgctcgatgt ccacaccgcg ctgaccgaca
    18001 atccgcacgc cgacactctc gcccgcgccg ttgccgaaaa ctgggaaacc gcgctgatcg
    18061 acgagttcca agacaccgac ccgctgcaat acgaaatctt ccaaaagatt ttcatcgccc
    18121 aaaaccgacc gctgttcctc gtcggcgatc ccaaacaggc gatttacagc tttcgcggag
    18181 cggacattta cgcctacctt caggcggcgg aagacgcgcg gcaccgctac acgctcgcca
    18241 ccaactaccg cagccacgcc gcgcttatcg gcagcatagg cgcgctgttc cgcctcaaag
    18301 aacgcccgtt cgttttggaa aacatcggct attcggaagt cggtgcggcg cgtgccgaaa
    18361 gcaggctgtc ccccgaacgt cctgccgtac aggttcgttg gctgcacgaa aacgacaatg
    18421 aaaaagccaa caaagacgtt ttgcgccgcc gtgccgccga ctattgcgcc gacgaaatcg
    18481 cccacgcgct caacgaagcc gccagaggcc gtctgaattt caaaggctgc ccgttgcagt
    18541 cgggcgatat tgccgtgctg gtacgcacgc acaacgaggc ggtgatggtt tccgccgccc
    18601 tgaaaaaacg gcaggtgcaa agcgtcctgc tttcgcgcga atctgtgttc gcctcgcccg
    18661 aagccgccgc cctgtccgca ctcatcggct tctggctcga accgcgccgc gccggaacgc
    18721 tgcgttttgt cctgacaagc agcatattcg gttatgacgc gcagcaattg cacgacttca
    18781 accaaaacga aagcgagatt ttgcattggg cggaatctgc ccgaaccgca ctcgacaact
    18841 ggaataaata cggcattttc gccgctatgc agcaattttc ccaaacacac ggcatcgaaa
    18901 cgcgcctctt aagccggaac aacgggcgca gtctgaccaa ttatttccaa ctgctcgaac
    18961 tgcttgccgc cgaagacgcg caaaaccgca accccgccgc gctgcacaaa tggctgggcg
    19021 accaaatcag ccttgccgaa aacaacagcg gcgacaaccg cgccatccgt ctggaaagcg
    19081 acgaagattt ggtcaaaatc gttaccatgc acgcctcgaa aggtttgcag tatccgctgg
    19141 tgtactgccc gtttgcgtgg gacgcgcaag ataccggacc gtccgactgg caaatcctcc
    19201 accaaggcgc aaaccgaacc gaactgttgg caaaggcgca actgtcggaa gacgaacaga
    19261 aacaatacgc cgatgaagaa atggcggaac gcctgcgcct gctttatgtc gcgctgacgc
    19321 gtgccgagga acagctcaac atctacgccg cgtactccac caatactgcc gacaaccccc
    19381 tcgcctacct gattgaaggc tcgccccaag acagccgtga aaccgtccgc cgtgcctatg
    19441 cgtgtgaaaa agacggcatc gcgatgctca aacgcaactg gcggcgcgtg gcggacaacg
    19501 ccccttccgg cacaaatttc gccttcaccg aaaatgcgcc gccccctgcc gcctatcgca
    19561 gcaacgccgg tcaaaccgcc gaatttgccg caaacagcat tcccgaacgc ggattccgat
    19621 ttgtccgcca cactagcttt accgccttaa gccgccatac ccaaacgccc gacggcggcg
    19681 aagaagatgc ctgcccgtcc ttggatgccg ccgaaacctc ggtgccggcg atgccgtctg
    19741 aaacgccgac ggcttcagac ggcatatcga tacacgattt tccgaaaggc acgcaggcgg
    19801 ggctgtgcct gcacgaaatt cttgaagatt tcaaattcgg acaagcagcc gccggacagg
    19861 aaaccctcat tgccgacaag ctgaaaaaat acggttttga agaaatatgg ctgcccgccg
    19921 ttgccgaaat ggcggaagcc tgccgcaaaa cgccgctgac gggggcatac gacctgtccg
    19981 acatctcacc cgagtgccgc tgtcccgaaa tgggctttac cctccacacc gaagacttca
    20041 gcctcaaacg cctgcgcgac tggtttgccc gcgacgacat caggctgccc gaagtctgcc
    20101 gtgccgctgc cgaaacgctc gacttccaca ccgtcaacgg ctttttaaac ggctttgtcg
    20161 atatggtctg ccaagacccc gacggcaata tctgcgtcat cgactacaaa tcaaaccacc
    20221 tcggtacgga cgcatccgcc tacacgcaac aggcaatgga cgaagccgtc gcacaccacc
    20281 actattacct tcaggcactg atttacgccg ttgccgccgc acgctacttc aagctgcgcg
    20341 gacaaccgcc cgccgccgtt tccgtccgct acctgttttt gcgcggattg gatggcaaag
    20401 gcggcggcgt ttggcgttgg gacatagatg ccgccgcttt ggaacagata aaataacttc
    20461 ccgtcctttc ggacaacaac cgcaggaaaa ccctatgaat atcaaacacc tgattaccgc
    20521 cgcactcatt gcctcagccg cctttgccgc gcaggcagcc ccgcaaaaac ccgtatccgc
    20581 cgcccaaacc gcgcaacatc cggccgtttg gatcgatgtc cgttccgaac aggaatttag
    20641 cgaagggcat ttgcacaacg cggtcaacat ccccgtcgac caaatcgtcc gccgcataca
    20701 cgaagccgcg cccgacaaag acacgccggt caacctctac tgccgcagcg gacggcgtgc
    20761 cgaagccgcc cttcaagagc tgaaaaaagc aggttataca aatgttgcca atcacggcgg
    20821 ttatgaagac ctgctcaaaa aagggatgaa atgacgcaat gccgtctgaa aggacaggct
    20881 ttcagacggc atacggcgtt ccggcacgcg gcgcaacatc gggcgcggcg cgcgccgccc
    20941 cgtccgaagc ctgataaaca aaccgataag gaacaatgat gtttcacgac acataaagca
    21001 ccgccctatg tgttgccctg atttggaaag ggttacaccc ctcccgaata aagtctgatt
    21061 ctaccgtttc aaccaaaaag gaaaaaggaa atacgatgaa acacatactc cccctgattg
    21121 ccgcatccgc actctgcatt tcaaccgctt cggcacatcc tgccagcgaa ccgtccaccc
    21181 aaaacgaaac cgctatgacc acgcataccc tcacctcaaa atacagtttt gacgaaaccg
    21241 tcagccgcct tgaaaccgcc ataaaaagca aagggatgga catttttgcc gtcatcgacc
    21301 atcaggaagc cgcccgccga aacggcttaa cgatgcagcc ggcaaaagtc atcgtcttcg
    21361 gcacgcccaa agccggcacg ccgctgatgg tcaaagaccc cgccttcgcc ctgcaactgc
    21421 ccctacgcgt cctcgttacc gaaacggacg gcaaagtacg cgccgcctat accgatacgc
    21481 gcgccctcat cgccggcagc cgcatcggtt tcgacgaagt ggcaaacact ttggcaaacg
    21541 ccgaaaaact gatacaaaaa accgtaggcg aataagcccg tcgccgacat atggcggatg
    21601 gaaatcaaat gcacacgccc ctcattccca cgaagtcggg aatccaatcg gttgggttac
    21661 cgtgattttc aatcattgcc gaaacggacg attccggatt cccacccttg cggggatgac
    21721 gggcgtgtgt gtttttgcca catcacaggc aactttgccg caaatcttat ctgtcgccgg
    21781 tatgccttct tgtttcaatc cgccacaaac caaatgccgt ctgaaacccg atttcagacg
    21841 gcatttccgt ttattccctg ctgtcgcggc aaatatcgat ggcttcctgc aaactcgaaa
    21901 cgccgtagat tttcaggttc ggaaactctt tggcgttgcg cggcatattg gctttgggga
    21961 cgatggcgcg tttgaagccg agtttttccg cttctttgag ccgctcttgc ccgcgtgcga
    22021 cggggcggac ttcgccgctt aagccgattt cgccgaaaac cacggttttt tcaggcatag
    22081 ggcggttgcg gaagctggaa agcatcgcga ggatgaccgc caaatccgcc gccggttcgc
    22141 cgattttcac gccgccgacg gcgttgagga acacatcctg atcgaaacag gcgatgccgc
    22201 cgtggcggtt taacacggca agcagcatcg caagacggtt ttgttccagt ccgacggtga
    22261 ggcgtttggg cgtgaagccg tgcgcgtcat cgaccaatgc ctgaatttcg accaaaagcg
    22321 ggcggctgcc ttcctgtgta accaaaacgc acgagccggg cgtatcgtcg cggtagctgg
    22381 cgaggaagat ggcggacggg ttggacacac ctttcaaacc gttttccgtc atcgcgaaca
    22441 cgcccagttc gtttgccgcg ccgaagcggt ttttgatggc gcgtatcatg cggtagttgg
    22501 aatgttggtc gccctcgaaa tacagcacgg tatcaaccat gtgttccagc acgcgcgggc
    22561 cggcaatcgc gccgtctttg gtcacgtgtc cgaccagtat catggcgatg cccatctgtt
    22621 tcgccatgcg cgtcagttgg gcggcacact cgcgcacctg cgacacggag ccgggggcgg
    22681 acgtgatttg gtcggaatac atggtttgga tagagtcgat gacgacaact tcgggctgat
    22741 gctgtttcaa ggccgcctga atcgcttcca tgcggatttc ggcaagaagg tttacgccgt
    22801 cggtcggcag ttccaaacgc tgcgcgcgca gggcgacttg ttgggcggat tcttcgccgg
    22861 aaacgtatag cactttacgg ctttgcgcca ttttggcgat ggtttgcaac agcagcgtgg
    22921 atttgccgat gccggggtcg ccgccgagca ggatgaccgc accatcgacc aaaccgccgc
    22981 ccaatacgcg gtcgagttcg cccataccgg tcggattgcg cggcacttcg gtggcggtaa
    23041 cggcggagag ggattggacg gtcgaggtat ccgccgccca agattggaaa cgggcgtttt
    23101 tcggctcggg cgcggcaagg ctttcctgaa gcgtgttcca ctcgccgcaa tgcgggcatt
    23161 tgccctgcca tttcggcgaa gtgccgccgc attcggtgca ttggtaaagg gttttaagcg
    23221 tttttgccat cggttttccc tgcatctgaa aacaaaaatg ccgtctgaac atagggacgg
    23281 ttgggattgc gccaatacga caacgccgcc gtcatgtact atccgtacac ggcggatatt
    23341 gccgcattgc cttattttta ccctaaccgg ccatctctcg gcttcagacg gcatatcggg
    23401 cggtttcctg ccggcttacc cgccgtgata ctgccgagac agctcgtgta cggtatcgac
    23461 taagattttg gcgtgttcgg ggtcggcgtg ttggttgatg ccgtgtccga ggttgaagac
    23521 atggccgctg ccgtgtccgt agtcggctag gatacgtgcg acctcggtgc ggatggattc
    23581 cggcgtaccg aagagggcga acgggtcgaa gttgccttgc agggcgactt gcttgccgac
    23641 gcggcggcgt gcttcgccga tgttacacgt ccagtccaag cccaatgcgt ctgcgccgat
    23701 ttgggccata ctttccagcc acagcccgcc gcctttggca aatacgataa caggcacgcg
    23761 gcggccttcg ctttcgcgtt tgagtccggc gacgatctgg cggatgtatt tgaggctgaa
    23821 ttctttaaac gccgcatcgc tcaacacgcc gccccaagtg tcgaaaatct gcaccgcctg
    23881 cgcgcccgcg tcgatttggg cgttgaggta ggcggtaacg gcttgggcgt tggtatcgag
    23941 gattttgtgc agcaaatcgg ggcgcgagta catcatggtt ttgatggtgc ggaattcttt
    24001 gctgccgccg ccttcgacca tataacaggc gagcgtgaac ggactgccgg agaagccgat
    24061 gagcggtacg cggccgtcca atgctttacg gatggaagtt accgcgtcga aaacgtattg
    24121 cagtttttcc atatcgggaa cgtgcagctt ggcgatgtcg gcctcgtgtt gcagggcgcg
    24181 tttgaatttc gggccttcgc cttcggcaaa atacagtccc aagcccattg cgtcagggac
    24241 ggtcaggatg tcggaaaaca aaatcgccgc gtccaaatcg aaacgttcca aaggttggat
    24301 ggtaacttcg gtcgccaatt cggtgttttt gcacaaatcg aggaagctgc ccgctttcgc
    24361 gcgtgtggct ttgtattcgg gcagataacg ccccgcctgg cgcatcatcc aaatcggcgt
    24421 gtattcgaca ggttgtttga gcagggcgcg gaggaaagtg tcgtttttca aagagatcat
    24481 gtggggcttt cggtttgttt gtaaaacggg gaaggaaaac ggttttcaga caacctttgc
    24541 ggcggtttcc gcaatgaaag gtcgtctgaa aagggaggga cgggcacatt tgcctaagta
    24601 atcatcagta gatgattaaa cggcagcagg ttttcatggg gtttcaatgg tgcgtttatc
    24661 ttcgatatgg ataactttgg gttcttcagc atcacaatcg cgatgagagc gcccttcgat
    24721 accgattttg aaatcttcat ccaatccttc ttcttttagg attagcgtaa aaatttcggg
    24781 atgatggcat gaaacggcat atccatcgcc actatctaaa gtgataatgc caaaatctgc
    24841 agatggtgtt cctatttgaa ccttgcccca tttgcttaac atctctttag cttgtttcgc
    24901 caaatctttt tccggttcaa gtaaaatgac gcaagtccca ttttcaaaga gcacccaaga
    24961 tttttcgttt ccattaatgc tctttttcca aacatcaatt aacttttggc gataagcagg
    25021 cgttccaatt tcaaatattt ctttcttttc cttaaaggaa tctgaagacc ccatcaaatt
    25081 ctcccagata aagatatttc ccaaaatcag ctatgctgct ctaacgctgc gtgacgttcg
    25141 tcatcggaga cggcttccaa aaccaagttg cgctgcgcct ccgccttctg cggttctccg
    25201 atttcgtcga aaacctttgc tagaaccaaa cgcgcggaaa tactcggctt taatgcaatg
    25261 ctcgcttcaa ggtagccttt tgccttgccc caaagtttgc ggccgtaggc gagccgaccg
    25321 agatacatca gcagaagcgc gttatcgggc tgttctttca gccaagcatc ggcaaaatcg
    25381 atggctttct gctgttcgcg ctcgcccaaa aagcgcacgc tttcgacaaa ggcttccaaa
    25441 agctcggggc ggcggttgtg cggataatgc tgtttgaccc atttgaccgc atcggcatac
    25501 agtcccaaac gttcgtactt ttccgcaacc gatacgctca attccccgtt tttgaggctg
    25561 tcgggaatcc gcttcaggca ggttttcaaa gcggcggcat cggcagcatc cgccagctgg
    25621 cggcggtatg cccaattttg ataccgttcc atttccgatt tgcccaacgc gcccgccttg
    25681 gaaagttttt cggtttttgc cagaacctgc aacgcgtcgc ccctgtcgaa agcgtaacga
    25741 agttgcagac gcacgaggcg cgtaaggttg gcattcatct tcgccgccgc atgaagattg
    25801 gcttccgccg cttcgtaatc gcgccggttc aacgccgatt ccgccaacaa aagataacgg
    25861 gaaagctgct gtttttccgg cagtttggcg atttccgcaa gataacggtc gcgcagctcg
    25921 atgttttcca tctgtccggc ggcgtgcgcg cccagcatca atgccaaagt ccggttgtct
    25981 ccggcctctt tgttgaccaa cacgcgtgag gcttctagtt ccgccttttc aaaacgccct
    26041 tcaaaatacg ccaaacccgc cttgttcaag gcaagcgcgg ccttgcggcc tttacgcgcc
    26101 gaaccgaaac gctgcatctt ttcggggata ttgagtacgc cgataatgaa tttaaacaag
    26161 aaataccaca ccacgacggc aatcagcgaa cctaacacaa aggcgtgcag gttgattctg
    26221 agcatggtct gtccgagtac gatatacacg tcgccggtgt aaatgcccga agccagcgcc
    26281 agtccgacgg cggcggcaaa caggacgaca atccagacta ccgttttcat gcgcgttctc
    26341 ctttagcgga cggcgaacgc cctgcctttt ccggtacggt ctgttttttt gcaggctgtt
    26401 ccggtttgcg ttccgaaggc agggacggtg cttctatggt cttcacatcc gatgcggcag
    26461 ccggttcgga agctgttttt tcgtttgccg gttcggaagc cgcctgttcc gcttcttgat
    26521 tttccgccgc cgtcatgcgc gtaccgtcgc gataggcgcg gacggcattt aggctgtttt
    26581 tcaaaccgtc atccgcagtc atccgcacat ccaacgcctt caattccgcc agttctttca
    26641 gccacgactg cgtggcggga gacttggcat cgaaatactg tctgacggcg gcttcggcat
    26701 tgttcaaatc gccctgatag acttcgctgt tgcgctgcat taatgcagtg cgcgcatcca
    26761 aaaggcggag gcgcaggttt tcacgcacaa aatatgcctg ttcgggagaa atcagcatgg
    26821 catcgttgtt ttccaaacgt cggatttcga ccagcccctt caatgtgccg agggattttt
    26881 cccatacgtt ctgccaccat gaagcggaag cggcttcgtt cttcacctgt acgcccggtt
    26941 tcagcacgcc gtcgagcatc agcggcagtc cggatacggc ggtttccagc ctgtcgaggc
    27001 gcaatgccgt gccggaaata tcgacatagg gacggttttt cagttccgcc aagtcgctgc
    27061 tgaccgcctg cttgatcggc agaagctctg cctgattgaa acgggacagg cggctgtcga
    27121 tatgctccaa tacgccgact gccgtttgga tattgccagt caacaccagc tgttgcgccg
    27181 ccagattgag tatggtctcg gtttcgtcca ccagccaatc ggcgcgtcct ttggtcaact
    27241 cgcgatagga tttttgcatt tccaagattt gttcgccgtt tgctttgacg tttccgtcca
    27301 aacggtcgag ctctgattgt atggcggctt gccggttgag gttgtctttc aacagggcgg
    27361 cgttttccga ctcgcccaag gcggctttgt cgattttttg gttgaatgcc agctcttggt
    27421 ttttcaagac attctgtcct tggacaaaca aaaaaccact tgtacccaaa cccaatgccg
    27481 ccaataccag ggcgcagact gccaaagcgt tgctgccgga ctgtttgacg ataaaaggag
    27541 cggcaggaat gtgtacttct gtttcgtttt ctttgcgtgg ggaagaggtt tcagacggca
    27601 tttcttttga tgcctgtatc tcgcgtacgg gttcggatga tttgttttca ggttcgccca
    27661 ccgttttgct ccttattaat ttgaggttcc gggaaagacc atgccgtctg aaacggaaat
    27721 ggaagaatgg gaaagcgcgg cttccagcgt agggacggtt tcgaccgaac acacgccttc
    27781 gcgcttcaat gcctccgcaa tgcgcggatg atgggtaaag tatagcaagg atttgaagaa
    27841 tcgggaaaat tgcggcggaa gctgcccgaa cagcaagcgc accagctcgg tcgacgtaat
    27901 ataggcggcg gcaatatttt cggtttggaa attttgaaag ttcaaaggtt tatgccgtct
    27961 gaaatagact tctgccacct ccgtccgaaa acctttctcc tgcaaggcat tcatcaaaaa
    28021 atcccgcccg ccgtgtccgc gcacaaacaa tacgcgcgca ccttcgggca gactgttcca
    28081 aaccggcagg cgcaaaaccg cctcgctgtc gttgccgtca tcaggcgcga tgaccgttct
    28141 gaccaaacag cgttccaatg cgcggcggct gccctgccct acggcaatct gcgcctttat
    28201 gccgtctgaa aggttaaggt acgggacggc ggtttcaacg gcggtcggac tgacccaaaa
    28261 cacggcatcc gcacgggcat attgttcgga caaaagttcc agtccggcag catctgcttc
    28321 gatttcgacc ggactcaata cttccgcgcg ccaaccggca ttcaggcaag tttcgacatc
    28381 ttctgcggcc ctgcccgacg gacggacaat cagcatgacg ggtttcgctg tttccatcgc
    28441 cttatccgcc ccgattacta ggaaaaatcg tcatcgttga aattcgcctg cgcggttttc
    28501 ggcgtacagc cgacccgtgc gacttccttg cccctacgct ccacaacgac accgctgctt
    28561 tccgtatgag tcacggaatc gaagcctgtc catatggtgt acataaattc gccgttttgg
    28621 aatttcatcg ttgcccaacg accgctgccc ataccttgcc acctgtcgga acgccccaac
    28681 aggtcagctt tgctgttgcg tatggcaatt tctttttttg ccgcactgcc gaacgaatat
    28741 tcgtaaagat tgcggttgat tttttggact tctatgtatt tgttgttgtc cgttttacag
    28801 gaaaacaccg tatccgccca cacggtttgg gcggcagccg acagaagcaa tacggcagaa
    28861 aggaacattt ttttcatcgt atttccttaa tggttgaaac cccgccactc ggacatccgt
    28921 ccttcgtggc agcagcatca gattttattt gaaaagggtg taaccccttc caaattaggg
    28981 caacacatag ggcggcgctt tatgtgtcgt cctgtgtgtt gaaacatcat tgcccgtatc
    29041 ctgaaataga aaagaaaatg cccgttttac ggtcttcttt ccgacaaaat gccgtctgaa
    29101 acggatttca gacggcatgg tcgaaaatta ttgcgcggaa agcggcttga gcaaccaggt
    29161 ttcaccgctt tgcacgacat tttccagcag atcgatgttc ggatgcttgc ccggatgggc
    29221 acgcgcgtcg gcaacgtgtt cggcaaacca tatcaaaccc tgttctgccg cttctttacc
    29281 caattttccg tcaaaacgtt ccgccaaagc attgtacagc ttgagcgaac cgagcttacc
    29341 ctgaacggca ggaatgtggt gaaccgtttt gccgtcggca tcctgaacgt ccaaaccgcc
    29401 cagatggtcg attgaaggaa atgtggcgag atagtcttga aaattcatat caactccgaa
    29461 aatcagtcag ttagcgcgtg ccgaaaatct tgtcgcccgc atcgcccaag ccggggatga
    29521 tgtagccgtt ctcgttcaag tggctgtcga gcgcggcggt gtaaatcgta acgtcagggt
    29581 gcgcgtcgtt gaccgccttc acaccctcgg gcgcggcaac cagcaccagt gccttgatat
    29641 ttttgcagcc cttggctttc aaaaggtcga tggtggcaac catcgaaccg cctgtcgcca
    29701 gcataggatc gataatcaaa gccggacgtt cgtccatact gtccacaaat ttctcaaaat
    29761 aggaaatagg cttcagcgtt tcttcgtcgc gctgcagtcc gactacactg attttggcag
    29821 tcggaatcag gtcgagcaca ccgtcaagca tacccaaacc tgcacgcagt atgggaacga
    29881 cggtcaatgt tttgcccttg atgcggtcgc cttcaatctg accgcaccat ccgtcgataa
    29941 ggtatttttc gatttcaaaa tcacggcttg cctcgtatgc catcaggcgc gccagctcgg
    30001 tggcaagcgt ccggaatttg taggtgctgc aatccgcctc cctcatcagg gttaatttgt
    30061 ggcggacgag cggatggttg ataacattaa cgttcatcgc ggtgttccaa tgtgattttg
    30121 agacgcgcca ttatactttt ttttgatttg ccggaaaaga aaatgccgtc tgaaacacgt
    30181 tcagacggca ttgccgaatc catcaggctt tcagcagctc ttgcagctcg cctacctcat
    30241 acatttccat caggatgtcc gaaccgccga caaactcgcc gttcacataa agttggggga
    30301 tggtcggcca gtcgctgtat tccttaatgc cttggcgcac ttcgggattt tccaatacgt
    30361 tgacggtaac gtaatcggtg cagcctgccg cgttcaggat ttgcacggcg cgggaagaga
    30421 aaccgcattg cggaaactgc ttcgtacctt tcataaacaa tacgacgcgg tgtgtcgtta
    30481 ctacttcttt aatttggtcg tggatggaag ccatcgctta ttccttttga taaagttgac
    30541 aaaatttgtc aggtagggcg cattatacgc aaaagcggaa acttcccaca atatttgccg
    30601 tatatcgcgc agtatcaata ttgtgaacgg aaatattccc actcctcgac aatcctgctg
    30661 tccggcaaat ggcacagggc atatccgccg ccgctttcgt ctttttttgc ttcccgtctg
    30721 ccgcctttgg caatgcagaa tttggaagcg gaatttgcca tgcctgtccg aaacgtgttg
    30781 gcaggcagcc ggcagcagcg cggacatgca tacggatgaa ttgccttcat ggttttcctt
    30841 atcgtcggta cagggtcaga cggtccgcat atcgggtact tttttaaaca gccggtaaaa
    30901 gttttccgtc gtatatgccg caacctgttc caatgtttgg ttccgcaatt tggcgatatg
    30961 ttcggcggta tggcgcacaa aagccggttc gttctgcctg ccgcgtttgg gaacgggggc
    31021 gaggaacggc gcatcggttt ccaccaaaat gcggtcgtcc ggcacatatt tcgccgcctc
    31081 ctgaaccaag ggtgcgtttt taaaggtaac gattcccgag aaagaaatat aaagccccaa
    31141 atccattgct gcacgggcaa aaccgatgtc ttcggaaaaa cagtggataa cgcccgaatt
    31201 aacccggcat tctttcagga tagacaaggt gtccgccgcc gcatcacgcg tatggacgat
    31261 aacgggcagt ccggtttgat tggctgcttc gatgtggtct gcaaagcgtt tgtgttgcca
    31321 ggacaaatcg cctttgcacc agtaataatc caaacccgtc tcgccgatgc cgaccacttt
    31381 cggatgggcg gcggcttcga ccatttccgc aatggaaaat tcttcggctt ccttgctgtc
    31441 gggatgtacg cctatggtgc aatagatgtg ttcgtgcgct tcggcgatgg caaagacttc
    31501 ggagaagctt tccctactga cgctgatggc gagtgcctgc cccacgccgt ttgcttccat
    31561 gttggacaaa acttcgggca ggcgttcttt caaaccttca aaattgaggt ggcagtgcga
    31621 atcgatgata tgcatggcgt tacatggtaa acgtaggtct gctgccgccg attgtgttgc
    31681 ccagttcgac ttcgatctgg tctttgactt tcaggcagtt ttcgtgtttt gtgaatgcca
    31741 gccccacccc tttgggtttg gaggaagtac gcgcaggatt gatccaggcg actttggtcg
    31801 gcaggaacag tttggggaag ttgaggattt ctacggcaag cagaatatcg tccccgatgg
    31861 aaaatacgtc gttggtctgc acaaacagac cgccgtgttc caaaaacggc atgtaggagc
    31921 tgtacagcag attcatgtct ttcagctgca acgacatcat ttttgccgga atattttgtc
    31981 cgtctgacat aattcacccg ttctctttct tcaattccaa atagttgatg agcagatgct
    32041 cgatctgcat tttgacattt aaagtatgaa acccgtaggg ggcaagctgt ttgagcatat
    32101 cctccgccgc aaatacattg cgcggacgga aaccggatac cgtctgaagc agcctgtctt
    32161 cataagcggg ataatagacg ggtttcatgt gttgcaggca caatcccaaa tcgaccagcc
    32221 atttctgcat ccacccgaca aatacggcga gcggaagttt ttccttatcg aaaagcgcgg
    32281 cgtaatccaa aatcttcaac aaccttggtt ctgccaaaat atccaacagt ttaatccgca
    32341 acgcacggac accgtccgcc tcatcaaaca gcggcgctcc ggaatggaaa gccagacgtt
    32401 cctcaggttc cgccacaccc ctttcacgca gatatgccaa tgcctcttca tgggaaggag
    32461 cgggcaaaac catcttccgg cagcgacttt taatggtcgg taaaaccttg tccgccgcgt
    32521 ggctgaccag caaaaagacc acttgtggcg gcggttcttc caacactttc aacaaactgt
    32581 tggcggcttg gacattcata ctttccgcag gatgaatcag aatcacgcgc aaaccgcccc
    32641 gtaccgaagt caggtacaca ttatcgatga tttccctgac ggcatcgatt ttgatctgca
    32701 acagtttgcg tccgttttcg ggttcgtccg acaagggggt gatttcgtaa aaatcgggat
    32761 ggcttccctg tccaaacaga tggcaggaca tacattcgcc acagggtttg cagcccggtg
    32821 caggggtttc gcacaacagt gccttcgccg caaagcgggc aaatgtagtt ttccccgtat
    32881 cttttttgcc ggcaaacagc catgcattgg gacgacgctc ccaatgttcc gcaatctgcc
    32941 gccattgctc attatgccac ggataaatca tttcaaacca tccgtccgga aatccatacc
    33001 gccatcagag cagttccaaa tcggggctgg aactttgcgc gcggcggctg cgcaactgta
    33061 ccgccaaacg caaatcgtgt gcggaatcgg catttttcaa tgcttcttgc agggaaatat
    33121 cgcctttttc atacaattgg taaaggtgtt gatcgaaggt ctgcataccc agggtagtgg
    33181 attttttcat cacttctttg atttcatgga tgttgccgtt gtgaatcaac tccgaaatca
    33241 ggggcgaatt gagcagcacc tcgactgccg ccaccctgcc cttgccgccg tctcgcggaa
    33301 cgaggcgttg cgaaataaac gcctgaaggt tgagcgacaa atccgtcagc aattgttcgc
    33361 gccgctcctc ggggaaaaag ttgatgatgc ggtcgagtgc ctgattggtg ctgttggcgt
    33421 gcagcgtcgc catacacaaa tgccccgttt cggcaaaggc aatggcgtag tccattgttt
    33481 cgcggtcacg gatttcgccg ataaggatga catcaggcgc ctgacgcagc gtgtttttca
    33541 acgccgccat ccagttttcc gtatccacgc cgacctcgcg ctgggtgatg atgcagtttt
    33601 tgtgttcgtg gacaaactcg atcgggtctt cgatggtgat gatgtgtccg aacgaatttt
    33661 cattgcggta gtcgataagc gaggcaagcg aagtcgattt acccgagccg gtgccgccga
    33721 caaaaataac cagcccgcgt tttttcagcg cgacatcctt caagactggc ggcaggttca
    33781 ggctttcaaa cttgggaatc ttgctggtaa tcgtacggaa taccaacgcc gtcgcgccgc
    33841 gctgtatcat cgcattgacg cggaagcggc tggtgtccgg caggctgatg gcgaagttgc
    33901 actcgttggt cgatgaaaat tcttccgcct gcttcgcact cataatcgaa aaggcgattt
    33961 ccatacattt ttccgccgtc agcggttcgt ccgtgatgcg ggtgattttg ccgtccagct
    34021 tcattgcggg cgggaaatgg gttgtcacga acaggtcgga acctttgttt tggttcatat
    34081 ggcgcagcca tgcgaacagt tcttcctttg cggtcatatt ttcatcggta aacatcctcg
    34141 tcccctaatc ttcaaacaat ctgtaccttc ccattctacc aaaaacccgc cgtgccgtct
    34201 gaaaccgttt cagacggcac ggacaaacca cctccactca atcctgccgt gttagcgtgt
    34261 ataatcaggc attctatccg aaattcacaa caggtcagca atatgtcttt gaaaacagta
    34321 gccgttatcg gcgcaatgga acaagaaatc gagcttttgc gcgagatgat ggaaaatgtc
    34381 aaagccgtct ctttcggcag attttccgcc tatgaaggcg aattggcggg aaaacgcatg
    34441 gtgcttgcat tgagcggcat cggcaaggtc aacgcggcgg ttgcaacggc ttggattatc
    34501 cgtgaattcg cagcggactg cgtcatcaac accggcagcg cgggcggttt gggcaagggt
    34561 ttgaaagtcg gcgacgtagt catcggcacg gaaaccgcac accacgatgt cgacgtaacc
    34621 gcattcggct atgcctgggg acaagtgccg caactgccgg caaggtttgc ttcagacggc
    34681 attttgattg aggcggcaaa acgggcggcg cggacgtttg aaggcgcggc ggtagaacaa
    34741 gggctgattg tcagcggcga ccgctttgtc cacagcagcg aaggcgtggc ggaaatccgc
    34801 aagcacttcc ccgaagtcaa agcagtggaa atggaagcgg cggcaatcgc ccaaacctgt
    34861 catcagttgg aaacgccttt cgtcatcatc cgcgcggttt ccgattcggc agatgaaaaa
    34921 gcagacatca gctttgacga gttcttgaaa acggcggcgg caaattccgc aaaaatggtg
    34981 gcagaaatcg tcaaatcttt ataagacctt tatcaaactt atgccaaaac agcggttttc
    35041 cgttagaata acgggctatt tccacgttgc acccgatatt cggacgcaac gccgtttgtt
    35101 cagacggcat ttcccaaacc cactgtccga cattcaaaac aactctaatg aaaaatatcc
    35161 gaaatttctc catcattgcc cacatcgacc acggcaaatc gacgcttgcc gaccgcttca
    35221 tccaatactg cggcggtttg gatttgcgcg aaatgagtac gcaggtgctc gattccatgg
    35281 acatcgaaaa agagcgcggc attaccatca aagcgcaaac cgccgcgctc aactataaag
    35341 cacgcgacgg gcaggtgtat caactcaacc tgattgacac gccgggacac gtcgacttct
    35401 cttacgaagt ttcccgttcg ctgtcggctt gcgaaggcgc gcttttggtc gttgacgcgt
    35461 cgcaaggcgt ggaagcgcaa accgtggcga actgctacac cgcgattgat ttgggcgtgg
    35521 aagtcgtgcc ggttttgaac aaaatcgacc tgcccgccgc cgaccccgaa cgcgtggaac
    35581 aggaaatcga agacatcatc ggcatcgatg ccgtcggcgc ggtgcaatgt tccgccaaaa
    35641 gcggcatcgg cgtggaagac gttttggaag aaatcgttgc caaaatcccc gcaccgaccg
    35701 gcgacgaaaa cgcgccgctg caagcggtta tcgtcgattc gtggtttgac aactatgtcg
    35761 gcgtggttat gctgattcga gtgaaaaacg gcaccatcaa actgaaagac aaagtgcgct
    35821 ttatgagcac caaggcggaa acgcaggtcg agcagctggg cgtattcaca ccgaaatcag
    35881 ttcaaaaaca agaactaaaa gccggcgaag tgggcttttt gattaccggc gtaaaagaat
    35941 tgggacaggc gaaagtcggc gatacggtta ctttggttgc caaccctgcc actgagccgc
    36001 tgcccggttt ccaagaggtg caaagccagg tattcgccgg actttatccc gtagaaagcc
    36061 acgactacga agctttgcgc gatgctttgg aaaaattgca gcttaacgat gcttcgttga
    36121 aatttgagcc tgaggtttcc caagcattgg gtttcggctt ccgttgcggc ttcttgggtc
    36181 tgttgcactt ggaaatcgtg caggaacgct tggagcgcga gttcgatatg gatttgatta
    36241 ccaccgcgcc gacggtgatt tatgaagtcg tgttgaaaag cggcgaaaaa atcgaagtcg
    36301 aaaatccgtc caaactgccc gacatcggca gcatcgaaac catactcgag ccgattatta
    36361 ccgcgaccat ccttgtgccg caggaatacg tcggcaacgt catgactttg tgtaaccaaa
    36421 agcgcggcgt gcaggtcaat atgcagtaca tgggccgcca agtgatgctg acttacgatt
    36481 tgcccatgaa cgaagtggtg atggactttt tcgacaaact caaatccact tcgcgcggct
    36541 atgcttcgtt ggattatcat ttcaaagagt tccaaccgtc tgatttgatt aagctggata
    36601 ttatggtcaa cggcgaaaaa gtcgatgcct taagcctgat tgtgcaccgt caaagcgcgg
    36661 ttcaccgagg ccgcgagcta gcatcgaaaa tgcgcgagct gattccgcgc caaatgttcg
    36721 acattgccgt ccaagccgcc atcggcagcc agattatcgc ccgcgaaaac gtcaaagcac
    36781 tgcgtaaaaa cgtcttggcg aaatgttacg gcggcgatat tacgcgtaag aaaaaactgc
    36841 ttgaaaaaca aaaggcaggt aaacgccgta tgaaacaagt gggcaatgtg gaaattccgc
    36901 aaagcgcgtt cttggcgatt ttgcaggtga gcgacaaata actccggcac acagggcggc
    36961 gcgtcgggaa cacccctcaa accgccctga tttcaggggc acgcgcccgc aaacaaattt
    37021 tgcaatttgc cgccttaaaa cggttgcacg gacaggcggg tttttaagca ttaaggaaca
    37081 acaatgaaca caatgctaat gtcgggcgcg gctgccgcgc tgcttgccgg catcatcctt
    37141 tatttcaaaa gcgacaagaa gcggcaggaa aacggggaat ggagttccgg ccttgaatac
    37201 gcctatatcc tgacagcggt cggcgtgttt gccgctttgt ccctgtttat gagctttacc
    37261 gccgttttcc tgattttcgt tgtattgtgc ggtacggctt ggggggtata taaataccgc
    37321 ctgaagactc atcccgaaat ctcggaaagc agccacttcg gcgattattt cggcagtttc
    37381 ttccctaccg ttttggtatt gttcctcatc cggtcgttta tcgccgaacc gttccaaatc
    37441 ccgtccagct cgatgcgccc gggcctgatc aagggcgatt tcattttggt cggcaaattt
    37501 tcctacggcc tgcgcgtacc cgttttaaac aatatattta ttcctacagg caaaatcgaa
    37561 cggggcgatg tcgttgtttt taattatcct ctgcagccgg agatgaccta catcaagcgt
    37621 attgtcggca ttccgggcga tgtggtcgaa tatcgggata agattttgac ggtaaatggc
    37681 aaacccactt ccgacattcc tgacggcaca taccgttatc ccgacgacac cgacccttcc
    37741 gaaatccaca acacggatat gttccgcagc ggtctagacg gcaaatcctt caatattctg
    37801 aaaaaagaag gacagcctgc cgtttccctg cccgtattgg gcaaatatac ctccgatatt
    37861 atgtctgaaa acggatattc catagagcaa agcggtttgg aacactgcca atatgccgac
    37921 gacggcagcg gtttcgtgtg caaagttccc gaaggacgct atttcgctat gggcgacaac
    37981 cgcgacaaca gtgccgattc gcgctactgg ggatttgtgg atgacaagct ggttgtcggc
    38041 aaggcaatgt tcattttgat gaacttcggc gatttcggca ggtccggtac ggcaatccgt
    38101 taggccagga agccaagaat gccgtctgaa aatagtttca gacggcattt gctattcctc
    38161 acttttacag gcgagtctaa cgttgtcgga ttttcctacc tatcagtcca atatacgcag
    38221 gttttgcacg gcgaactgcc aatattgccc tgatgaaatc aatgtgaaga ctgccgtttc
    38281 agcgaaccga ggtaaattgc cgaaagcgtc agcgccaccc ctgcccactg cacgccttca
    38341 atcggtttgc ccaacccgaa ataatcgatg aacagggcgg caaccggttc ggacaaaagc
    38401 agcagccccg tcagcgacag cgaaagcagc ggaatcgcat aggcaaccat cgcccacgcg
    38461 aagcactgca tcaccacgcc gtacacaagc accaaacccg catctttcca agtcgtcgga
    38521 taaagcgcgc cgccatccat cagcaatgcc ggaacaacca gcgataccgc gccgcccaaa
    38581 ctcaaaatca tcattgaagg gaaaagcgcc accggctcga tttcatgggt tttgcggaca
    38641 aacaccatcg acagggcgag catcagtccc gataccaaac cgctggcgaa tccccaaacc
    38701 gcattaccgt tgtagccgaa ttccgcaccg gcaatcatcg ccacgccggc aactgccgat
    38761 attaagcctg cctttttcag cccgctcaaa cgctcgccga aaaagaaaac accgattgcc
    38821 gacaagaaaa agatttgcag gctgttgagc agggtggaaa tacccggccc gaccgcgtgt
    38881 atgctttcgt gccacaacgc caaatcgaaa gcgagaaaca cgcccgccgt cagggcatat
    38941 cggacggttt tcctgttttt tgggaatttt tgcctgaaaa accgtgctaa aaaccaaaat
    39001 acgaacaccg aaatcagcaa ccgccaaaat gcgattgcat acgaaccgac ggggacggat
    39061 ctgacaatca gactgcccag accgaacacc acgcaaccca gcaccagcaa tggtgcggca
    39121 tattttttgc cgttaccgac cacaatatcc cctcccttcc ccatacggct gccgcgccga
    39181 cggcaagaat gccgccattc cgaaaatatg ccgtctgaag cccaaagccc cggctgcacc
    39241 cggacaaacg gctgatttta cgggcaggca ggctattttc cgttggggca aaaaaccgtc
    39301 ctgccaaata aaaccc
//
LOCUS       DAWIVB010000003        35309 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWIVB010000003 DAWIVB010000000
VERSION     DAWIVB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.003"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            155..1123
                     /locus_tag="WIX60_000088"
     CDS             155..1123
                     /locus_tag="WIX60_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ5304423.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIVGRVIVFPFEIPVSTVFGVMGTALFLWLLLRKPAHAV"
     gene            1113..2087
                     /locus_tag="WIX60_000089"
     CDS             1113..2087
                     /locus_tag="WIX60_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ5304424.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTLGGV
                     GYTSLPAVGKFGFELAVMMGGSLLLFYTLVKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFASFNTVHNELLGIGALILLISAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2132..2758
                     /locus_tag="WIX60_000090"
     CDS             2132..2758
                     /locus_tag="WIX60_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304425.1"
                     /translation="MTQEHFPSFFNQAPTITVRDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRHLLSFGVGEINGTLTLRRKDNGKTVAVGLNAALQPFAPE
                     MRDIMPKAVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            2843..3601
                     /locus_tag="WIX60_000091"
     CDS             2843..3601
                     /locus_tag="WIX60_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5304426.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADYVVAMKNGQVAMQG
                     KPNDIFTAENIKTLFDMDVNVLDYEGKKLVIHHI"
     gene            3893..4980
                     /locus_tag="WIX60_000092"
                     /pseudo
     CDS             3893..4980
                     /locus_tag="WIX60_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NadA"
     gene            complement(5046..5384)
                     /locus_tag="WIX60_000093"
     CDS             complement(5046..5384)
                     /locus_tag="WIX60_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ5304427.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKIKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            5535..9497
                     /gene="purL"
                     /locus_tag="WIX60_000094"
     CDS             5535..9497
                     /gene="purL"
                     /locus_tag="WIX60_000094"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ5304428.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGRLNDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSGVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDARRGAVFKLREVPLEEHGLNPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKLPKTTRTDKTVAPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGLTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYSVIQQLVAENKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRIHLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYIDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRITIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG
                     "
     gene            9592..10350
                     /gene="gloB"
                     /locus_tag="WIX60_000095"
     CDS             9592..10350
                     /gene="gloB"
                     /locus_tag="WIX60_000095"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ5304429.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            10664..14959
                     /gene="mspA"
                     /locus_tag="WIX60_000096"
                     /gene_synonym="ausI"
     CDS             10664..14959
                     /gene="mspA"
                     /locus_tag="WIX60_000096"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ5304430.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFTVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLVHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPTALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGNSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMKNSTSWQFIGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMNLN
                     GEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAGRN
                     TAHLDGDITAYDLSGIDLGFTQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPATQV
                     RGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQIT
                     LNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQIHV
                     KNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNPLK
                     EAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARIDS
                     QVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSADICRQVAKAADTNDLTLFETELDTY
                     IERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNLAE
                     LISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHRPY
                     QQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENGAL
                     FAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSNGN
                     RYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALSVN
                     DRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            15191..16645
                     /gene="mgtE"
                     /locus_tag="WIX60_000097"
     CDS             15191..16645
                     /gene="mgtE"
                     /locus_tag="WIX60_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ5304431.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVWDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(16731..17639)
                     /gene="hslO"
                     /locus_tag="WIX60_000098"
     CDS             complement(16731..17639)
                     /gene="hslO"
                     /locus_tag="WIX60_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ5304432.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLVNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAGLDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            17884..18639
                     /locus_tag="WIX60_000099"
     CDS             17884..18639
                     /locus_tag="WIX60_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ5304433.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            18658..18876
                     /locus_tag="WIX60_000100"
     CDS             18658..18876
                     /locus_tag="WIX60_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304434.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            19021..19365
                     /locus_tag="WIX60_000101"
     CDS             19021..19365
                     /locus_tag="WIX60_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ5304435.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            19365..20057
                     /locus_tag="WIX60_000102"
     CDS             19365..20057
                     /locus_tag="WIX60_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ5304436.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            20124..21344
                     /gene="argJ"
                     /locus_tag="WIX60_000103"
     CDS             20124..21344
                     /gene="argJ"
                     /locus_tag="WIX60_000103"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ5304437.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFNEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            21404..22813
                     /locus_tag="WIX60_000104"
     CDS             21404..22813
                     /locus_tag="WIX60_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ5304438.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            23003..24394
                     /gene="hrpA"
                     /locus_tag="WIX60_000105"
     CDS             23003..24394
                     /gene="hrpA"
                     /locus_tag="WIX60_000105"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ5304439.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKAIAKNQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIRETAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMAALKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            24538..26181
                     /locus_tag="WIX60_000106"
     CDS             24538..26181
                     /locus_tag="WIX60_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ5304440.1"
                     /translation="MDEIQIPKKVELQTKLENEKIVLSKGSTTIIVGANGTGKTRLAV
                     YIEEQLKEKAHRISAHRALKLNPNVNKIPEKSAKTYLSYGQNWDGIDVSNRKNYRWDN
                     NSYTHLLNDFDWLLQYLFAQQNNIAVANNQKLNRNEKVTNSKTKLDILQEAWETLLPH
                     RKLHITADDIQVSAVDNEELYSASNMSDGERALFYILGQVLSVDDGSVLIFDEPELHI
                     HKSIISNLWDKIEELRPDCSFLIITHDIEFAATRVAKKYVIRNYYPTPAWDISEVPES
                     NFDEETITMILGSRKPILFVEGNNNSLDIATYRYCYPDWTIIPKGACKDVIQSVSSLK
                     KLSNEMPLLNLKCSGIVDLDSRDEREIEQLNNLGIYILPVSEIENLFSLTDVAKEILK
                     LNQYSDEELLNKLNGFKSELIKYIDNELKDDKLDEFVVKQVRRKIDNYLKNIDLSSKI
                     TSTDMKKSLLNEISTLTEQKIETWISEIKNEIQRCIEQQDLDKLLTIYDNKGLLAKSA
                     CVLKGMRNKHEFESWIMRTLKGRNKDFIDAIRQKLPILD"
     gene            26225..26407
                     /locus_tag="WIX60_000107"
     CDS             26225..26407
                     /locus_tag="WIX60_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304441.1"
                     /translation="MKNHQTTLSFPTSSSLKPCADKQGLSEDRSQYRLTKLGEQMAHL
                     PIDPKIARILLVLFRF"
     gene            26619..28196
                     /gene="lpt3"
                     /locus_tag="WIX60_000108"
     CDS             26619..28196
                     /gene="lpt3"
                     /locus_tag="WIX60_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ5304442.1"
                     /translation="MMKKSFLTLVLYSSLLTASEIAYRFVFGIETLPAAKIAETFALT
                     FVIAALYLFARYKVTRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPVLWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGKHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            28298..31681
                     /locus_tag="WIX60_000109"
     CDS             28298..31681
                     /locus_tag="WIX60_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ5304443.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQIARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDASAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEVRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYAAQQTTPSPVGEGRGEGKTVAAQTNFSATAANPLPNPLP
                     QEREQSAAASTISDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATSTNPLPQERE
                     QSASASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATSTLSDDSKPKKQPA
                     PQKNRLKPLPLADIRTFQAWLKTAERDNPRLLFLSRDDLMQHAAAHITEEQFPKFWQT
                     ADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMIREKIQLQIKALPK
                     QIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRIFEQINQDEWAAFRL
                     PEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDNVTAWDIGT
                     LPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQ
                     VKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKR
                     ARSRLPAVKEALSRYLQETAAVYAELNSKLGKHPLTHLLRLRLQTLLAAGFATRTPWA
                     QWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDSLIKQGLPISDGL
                     AAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEKIEK"
     gene            complement(31714..32805)
                     /locus_tag="WIX60_000110"
     CDS             complement(31714..32805)
                     /locus_tag="WIX60_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ5304444.1"
                     /translation="MQTFNRERLEFALDKRGCTQTKLAELLGITARQVRNYVKDEQIP
                     DLNELANLLEFPVHFFTNDERLPELKSQAVSFRARSRTSKRLEKQALNHSITAFLLND
                     WLETEFTLSQAVLPDFSDISPEEAAQELRLEWGLGNEPIPNMITLLEVKGVRVFSLSL
                     DTKDIDAFCTWYENHPFVFLNTQKSAERSRFDAAHELGHLIRDKYSMEHSKNSEITED
                     EPRDIIEKEANAFASAFLMPEAALRLYRHVPITIENLLKIKRRFGVSLVALAYRMHKL
                     GMITDRMYIHNLCPLFAKKKYRTIEPEPMEREISSALRKMLTILKNDGIGIEQIAENL
                     NVSPKDVADLTFGLVERDLNRYRKLRLVK"
     gene            complement(32821..33465)
                     /locus_tag="WIX60_000111"
     CDS             complement(32821..33465)
                     /locus_tag="WIX60_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304445.1"
                     /translation="MVEFALSEPFPFEVGEIVEQQNSDIALSEISKYLSQEMLKEIVL
                     TAHNNIQDKAALSDRNTLGMYRQAEITFALRVYLSKDGWTLEPWSGGAFLCLSPCRTK
                     SIIVATATSDVGRHHGMPMTTARKGKGFEEAIRNSKLDLPDTVQFWVLLFHWKYNKVQ
                     LELSLPSGFVSHQITGYKQRIILADVDLTKFDELERKQPVEEYEPLVERKQKAG"
     gene            33822..33974
                     /locus_tag="WIX60_000112"
     CDS             33822..33974
                     /locus_tag="WIX60_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304446.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWV"
     gene            34105..34500
                     /locus_tag="WIX60_000113"
     CDS             34105..34500
                     /locus_tag="WIX60_000113"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ5304447.1"
                     /translation="MPSENIFRRHYKICSPFQMSNVHPYTIFATMPHKSTAKDILIMS
                     YDLSKRLVIGLASSALFDLSESDNIFRMEGAETYRQYQREKQNHPLKKGVVFPFIKKL
                     LSINEINPNDPTIGFILLSRNNPDTDYES"
     gene            complement(34590..35039)
                     /locus_tag="WIX60_000114"
     CDS             complement(34590..35039)
                     /locus_tag="WIX60_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ5304448.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
     gene            complement(35127..>35309)
                     /locus_tag="WIX60_000115"
     CDS             complement(35127..>35309)
                     /locus_tag="WIX60_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEZ5304449.1"
                     /translation="AKAKAIAEYRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGA
                     PAPKGPAKPAIPAAKK"
BASE COUNT         9100 a        10189 c         8366 g         7654 t
ORIGIN      
        1 cgaggcaacg ccgtactggt ttttgttaat ccactatatt ggaacttgcc ctatcctctg
       61 cccgatacgg aggcttcaaa caaggctttc cgctccgacg gttcggactg ccttgtttga
      121 atcttctacg ccttaacgct tttcccttct gtttatgact gccaaacctt tttccctcaa
      181 cctgaccaac ctcctgctgc tggcggtgtt gtttgccgtc agcctgtcgg tcggcgttgc
      241 cgatttccgc tggtcggatg tgttttcgct gtcggacagc cagcaggtta tgttcatcag
      301 ccgcctgccg cgcacgtttg cgattgtgct gacgggcgcg tcgatggcgg tggcggggat
      361 gattatgcag attttgatgc gtaaccgttt tgtcgagcct tctatggcgg gcgcgggtca
      421 aagcgcggct ttgggtttgc ttctgatgtc cctgctgctg cctgccgcgc cgctgccggt
      481 caaaatgtcg gttgccgccg ttgccgcgct gatcgggatg ctggtgttta tgctgctgat
      541 ccgccgtctg ccgccgacgg cgcaactgat ggtgccgctg gtggggatta ttttcggcgg
      601 cgtggttgag gcagtggcga cgtttatcgc gtatgaaaac gaaatgctgc aaatgctcgg
      661 cgtgtggcag cagggcgatt tttcgagcgt gctgctgggg cggtacgagc tgctttggat
      721 tacgggcggt ttggcggtgt ttgcctatct gattgccgac cggctgacga ttttggggct
      781 gggcgagacg gtcagcgtga atttgggttt gaaccggacg gcggtgttgt ggtcggggct
      841 gattattgtg gctttgatta cgtcgctggt tatcgttacg gtcggcaata tcccgtttat
      901 cgggctggtc gtgccgaaca tcatcagccg cctgatgggc gacaagctgc gacaaagcct
      961 gcctgcggtg gccttgctgg gcgcatcttt ggtgttgctg tgcgatattg tcggacgcgt
     1021 gattgtgttt ccgtttgaaa ttccggtctc tacggttttt ggcgtgatgg ggacggcttt
     1081 gtttttgtgg cttttgttga ggaaacctgc tcatgccgtc tgaaaaaaat atcggtttta
     1141 tggcaggaag cagccgtccg ttgtgggtcg cctttgcgct gttgctggtt tcctgcgtcc
     1201 tgtttatgac gctcaacgtc aaaggcgatt gggattttgt tttgcacctg cggctgacca
     1261 aacttgccgc gctgctgatg gtcgcctatg cggtcggcgt gtccacgcaa ctcttccaaa
     1321 cgctgaccaa caatccgatt ctgacccctt cgattttggg ttttgattca ctgtatgtgt
     1381 ttttgcagac cttgctggtg tttacgctcg gcggcgtggg ctatacgtcc ctgcctgcgg
     1441 tcggcaagtt cggcttcgaa cttgccgtca tgatgggcgg ctcgctgctg ctgttctaca
     1501 cgctcgtcaa acaaggcggg cgcgatttgt cgcgcatgat tttaatcggc gtgattttcg
     1561 ggattttatt ccgcagcctt tcctcgctgc tctcccgcat gatcgatccc gaagagttta
     1621 ccgcggcgca ggcaaacatg ttcgccagtt tcaataccgt ccacaacgaa ctgttgggga
     1681 taggcgcgct gattctgctc atcagcgcgg cggcggtttg gcgcgaacgc taccgcttgg
     1741 acgtacacct tttggggcgc gaccaagccg tcaatttggg catcagctac acgcgcaaca
     1801 ccttatggat actgctttgg attgccgcgc tggtggcgac ggcgaccgcc gtggtcggcc
     1861 cggtgagctt tttcgggctt ctcgccgcct cgcttgccaa ccacttttcc ccgtccgtgc
     1921 gccattccgt ccgcctgccg atgacggttt gtgtcggcgg tatcctcctg gtcggcggac
     1981 aaaccgtatt cgaacacttc ttgggcatga aggcggtatt aagcgtggtg gtcgaatttg
     2041 cgggcggact cgttttcctc tatctcgttt taaaacacaa aaaatgacgg atgccgtctg
     2101 aacggccgcc cgcctcgaaa ggacaaacca tatgacacaa gaacatttcc catcattctt
     2161 caaccaagcc ccgaccatca ccgttagaga cccgcttgcc gcattcctcg gcgcggccga
     2221 aaacggcatc ctcacttacc gctacgccga tgccgtgcgc ctgtgcggac attcctgccc
     2281 gaccgtcgcg ggcgcgtacc tgatggttat caaaggtctg aaagcccttt acggcgaaga
     2341 gctgcccgaa cgcggcggca tcgaagccgc catgcaggga gcgcgcgacg aaggcacggt
     2401 cggcgtaacc gcgtccgtcg tccaactcct caccggcgca gcccccgaaa ccggctttgg
     2461 cggcatcgga atgcagggac gctttgcccg ccgccacctc ttatcctttg gtgtaggcga
     2521 aatcaacggc acactgaccc tgcgccgcaa agacaacggc aaaaccgtcg ccgtcggcct
     2581 caacgccgcc ctgcaaccct tcgcacccga aatgcgcgac atcatgccca aagccgtcgg
     2641 cggcagcgca agcgcagaag aactcgaacg cttcggacaa ctctggcagg cacgcgttaa
     2701 agcatttttg accgaatcgg cggacgaccc gcagttcgtc atcgtccgcg aagtgtgagc
     2761 gttcagacgg cattccgaat ttcaaatgcc gtctgaaccc ccgccaaaca tacaacaaac
     2821 ctacgcccga caagcatccg ccatgattac catccgcaac gtcagctacc gcatcggcac
     2881 acgccccatc ctcgacaacg tcagcctcga catccccgaa ggcggcatta ccgccctcgt
     2941 cggcccgaac ggcgcaggca aatccaccct gttttccttt atggcgcggc tgcgcccgct
     3001 tgaaagcggc agcatcgcct accgaggcaa aaaccttgcc ggcactccca ccgccgaact
     3061 cgcccgcacg ctctccatcc tcacccaaga aaacagcatc atgagccgca tcaccgtgcg
     3121 cgacctgctg atgttcggac gttaccccta ccatcaaggc agaccgactg ccgaatgccg
     3181 ccgtatcgtt aacggtgcaa tcgaagaatt ccacctgcag gacctctccg accgctatct
     3241 gaccgaactc tccggcggac aacgccaacg cgccatgatt gcgatggtgt tctgccaaag
     3301 caccgactac gtccttttgg acgaaccgct gaacaacctc gatatgtatc acgcccgctc
     3361 gctcatgcag attctgcaaa agctcacgcg cgaacacaaa cgcaccaccg tcgccgtatt
     3421 gcacgacatc aaccaggccg ccgcctacgc cgattacgtc gtcgccatga aaaacggcca
     3481 agtcgccatg cagggcaaac ccaacgatat tttcaccgcc gaaaacatca aaaccttatt
     3541 cgatatggac gtcaacgtcc tcgattacga aggcaaaaaa ttggttatcc accatatcta
     3601 aatccgacaa aaaggccgtc tgaacattca gacggcaacc catatcccga caaaattaag
     3661 acacgacacc ggcagaattg acatcagcat aatatgcaca tattaacaga tattaatgcc
     3721 gaactaccta actgcaagaa ttaaataaat aaataaataa ataaattgcg acaatgtatt
     3781 gtatatatgc ctcctttcat atatacttta atatgtaaac aaacttggtg gggataaaat
     3841 acttacaaaa gatttccgcc ccatttttta tccactcaca aaggtaatga gcatgaaaca
     3901 ctttccatcc aaagtactga ccacagccat ccttgccact ttctgtagcg gcgcactggc
     3961 agccacaagc gacgacgatg ttaaaaaagc tgccactgtg gccattgttg ctgcctacaa
     4021 caatggccaa gaaatcaacg gtttcaaagc tggagagacc atctacgaca ttggtgaaga
     4081 cggcacaatt acccaaaaag acgcaactgc agccgatgtt gaagccgacg actttaaagg
     4141 tctgggtctg aaaaaagtcg tgactaacct gaccaaaacc gtcaatgaaa acaaacaaaa
     4201 cgtcgatgcc aaagtaaaag ctgcagaatc tgaaatagaa aagttaacaa ccaagttagc
     4261 agacactgat gccgctttag cagatactga tgccgctctg gatgaaacca ccaacgcctt
     4321 gaataaattg ggagaaaata taacgacatt tgctgaagag actaagacaa atatcgtaaa
     4381 aattgatgaa aaattagaag ccgtggctga taccgtcgac aagcatgccg aagcattcaa
     4441 cgatatcgcc gattcattgg atgaaaccaa cactaaggca gacgaagccg tcaaaaccgc
     4501 caatgaagcc aaacagacgg ccgaagaaac caaacaaaac gtcgatgcca aagtaaaagc
     4561 tgcagaaact gcagcaggca aagccgaagc tgccgctggc acagctaata ctgcagccga
     4621 caaggccgaa gctgtcgctg caaaagttac cgacatcaaa gctgatatcg ctacgaacaa
     4681 agctgatatt gctaaaaact cagcacgcat cgacagcttg gacaaaaacg tagctaatct
     4741 gcgcaaagaa acccgccaag gccttgcaga acaagccgcg ctctccggcc tgttccaacc
     4801 ttacaacgtg ggtcggttca atgtaacggc tgcagtcggc ggctacaaat ccgaatcggc
     4861 agtcgccatc ggtacggctt ccgctttacc gaaaactttg ccgccaaagc aggcgtggca
     4921 gtcggcactt cgtccggttc ttccgcagcc taccatgtcg gcgtcaatta cgagtggtaa
     4981 gcagcatctc ccgataaaga aaccgcagcc ctgcaaggct gcggttttta ttttctatcc
     5041 ggccgtcaga ctgccgcgtc cgaacgttcg cccgtgcgga tacggattgc ctcctcaacc
     5101 ggcagcacaa aaatcttgcc gtcgccgatt tttcccgaac gcgccacctc gacaatcacg
     5161 tcaatcgcgc gttccacagc atcatccgcc aacaccagct cgattttgat tttgggcagg
     5221 aaatcgacgg cgtattccgc gccgcgatag atttccgtat gccccttctg cctgccgaac
     5281 cctttgacct cgctgacggt catgcccgta atgccgattt ccgtcaacgc ctcgcgcacg
     5341 tcgtcgagtt tgaacggttt gacaatcgcc tcgatttttt tcataaaatt tcctttgaac
     5401 aaacaataca aacacatccg aaaaacggga acctcccgtc agattgtcaa cattttaagc
     5461 caaaataccc aagcaataca gccccgttgc gcgtataatg acagattttc caaccgcatt
     5521 tgagagccga atccatgtct gtcgttttgc ccttgcgcgg cgttaccgcc ctttccgatt
     5581 tccgtgttga aaaactcttg caaaaagccg ccgcactcgg tctgcccgaa gtcaaattaa
     5641 gcagcgaatt ttggtatttc gtcggcagcg agaaagcact tgatgccgcg actgtcgaaa
     5701 aactgcaagc cttgttggcg gcgcaaagcg ttgaacaaac gccaaaagcg cgcgagggct
     5761 tgcatttgtt tttggtcacg ccccgtttgg gtacgatttc gccgtgggct tccaaggcga
     5821 ccaatatcgc ggaaaactgc ggtttggcag gcatcgaacg catcgagcgc ggtatggcgg
     5881 tgtggctgga aggtcgtctg aacgatgaac agaaacagca atgggcggct ttgctgcacg
     5941 accgcatgac cgaaagcgtg ctgcccgatt ttcagacggc ctccaaatta ttccaccatc
     6001 tcgaatccga aactttctcc ggcgtcgatg ttttgggcgg cggtaaagaa gctttggtca
     6061 aagccaatac cgaaatgggc ttggcacttt ccgccgacga aatcgattat ctggtcgaaa
     6121 actatcaggc tttgcagcgc aatccgtccg atgttgaatt gatgatgttc gcgcaggcaa
     6181 acagcgaaca ctgccgccac aaaatcttca acgccgattt catcctcaac ggcgaaaagc
     6241 agcccaaatc cctcttcggt atgatacgcg acacacacaa cgcgcatccc gaaggcacgg
     6301 tcgttgccta taaagacaat tcgtccgtaa tcgaaggcgc gaaaatcgag cgtttctatc
     6361 cgaatgcggc ggaaaaccaa ggctaccgtt tccacgagga agacacgcat atcatcatga
     6421 aagtggaaac gcacaaccac ccgaccgcca tcgcgccgtt tgcgggtgcg gcgacgggcg
     6481 cgggcggcga aatccgcgac gaaggcgcga cgggcaaagg ttcgcgtccg aaagcgggcc
     6541 tgaccggctt taccgtgtcc aacctcaata ttcccgacct caaacagccg tgggaacaag
     6601 actacggcaa gccggaacat atttcctcgc cgctggacat catgattgaa ggcccgatcg
     6661 gcggcgcggc gttcaacaac gaattcggcc gccccaacct cttgggctac ttccgcactt
     6721 ttgaagaaaa atttgacggt caggttcgcg gctatcacaa accgattatg attgccggcg
     6781 gcttgggcag cattcaggcg cagcagacgc ataaagacga aatccccgaa ggcgcattgc
     6841 tgatccaact gggcggcccg ggtatgctta tcggcttggg cggcggcgcg gcttcttcga
     6901 tggataccgg cacaaacgac gcgtctttgg acttcaactc cgtgcaacgc ggcaaccccg
     6961 aaatcgaacg ccgcgcgcag gaagtcatcg accgctgctg gcagctcggc ggcaaaaacc
     7021 cgattatctc catccacgac gtaggcgcgg gcggcctgtc caacgccttc cccgaactgg
     7081 tcaacgatgc cagacgcggc gcagtattca agctgcgcga agtgccgctt gaagaacacg
     7141 gcctcaaccc gctgcaaatc tggtgcaacg aatcgcaaga gcgttatgtg ttgtcgattt
     7201 tggaaaaaga tttggatgct ttccgcgcca tctgcgaacg cgaacgctgc ccgtttgccg
     7261 tagtcggcac ggcgactgac gacggtcatt tgaaagtacg cgacgatttg ttcgccaaca
     7321 atcccgtcga tttgccgttg aacgtcttgc tcggcaaact gcccaaaacc acgcgcaccg
     7381 acaaaacggt tgcaccgtcc aaaaaaccgt ttcacgcggg cgatatcgac attaccgaag
     7441 ccgcctaccg cgttttgcgc ctgcctgccg tagccgccaa aaacttcctg attaccatcg
     7501 gcgaccgcag cgtcggcggt ttgacgcacc gcgaccaaat ggtcggcaaa tatcaaactc
     7561 cagtagccga ctgcgccgtt accatgatgg gcttcaacac ctatcgcggt gaagcgatgt
     7621 ctatgggcga aaaaccgacc gtcgccctgt ttgatgcgcc tgcttcgggc agaatgtgcg
     7681 tcggcgaagc catcaccaac atcgcggcgg tcaacatcgg agacatcggc aacatcaaac
     7741 tctccgccaa ctggatggcg gcgtgcggca acgaaggcga agacgaaaaa ctctaccgca
     7801 ctgtcgaagc cgtttccaaa gcctgtcagg cattggattt gagcatcccc gtgggcaaag
     7861 acagcctgtc gatgaaaacc gtttggcagg acggcgagga gaaaaaatcc gtggtttcac
     7921 cgttgagcct gattatctca gcgttcgcgc ctgtgaaaga cgtacgcaag actgttacgc
     7981 ctgagttgaa aaacgtcgaa gacagcgtat tgttgtttgt cgatttgggc ttcggcaaag
     8041 cgcgtatggg cggttcggcg tttggtcagg tgtacaacaa tatgagcggc gacgcgcccg
     8101 atttggacga tacaggtcgt ctgaaagcct tttacagtgt gattcagcag cttgttgccg
     8161 aaaacaaact cttggcgtat cacgaccgca gcgacggcgg cttgtttgcc gttttggtag
     8221 aaatggcgtt tgcggggcgg tgcggcttgg atatagattt aaatttattg cttgcacaaa
     8281 catttattac caaccatacc gctctgtctc aatcattgcg gactgaagag gtaaaagcgt
     8341 tggctgaatg gcaagaaacc attgcccgca cattatttaa tgaagagttg ggtgctgtta
     8401 tccaagttag aaaacaagat gttgccgata ttatcaattt attctatcaa caacagctgc
     8461 atcataatgt ctttgaaatc ggtacgttaa ctgatgagaa cacgttaatc atccgcgacg
     8521 ggcaaacgca ccttatttct gacaacctaa tcaaactgca acaaacctgg caagaaacca
     8581 gccatcaaat ccaacgcctg cgcgacaacc ctgcctgcgc cgacagcgag ttcgcactga
     8641 ttggcgacaa cgaacgcagc gcattgtttg ccgacgtgaa gttcgacgtg aacgaagaca
     8701 tcgccgcgcc gtttatcaac agcggcgcga aacccaaaat cgccatcctg cgcgaacagg
     8761 gcgtaaacgg gcaaatcgaa atggccgccg cctttacccg cgccggattc gatgcttacg
     8821 acgtgcatat gtccgacctg atggcaggcc gcatccacct cgccgacttc aaaatgctgg
     8881 cggcgtgcgg cggcttcagc tacggcgacg tactcggcgc gggcgaaggc tgggcgaaat
     8941 cgattctgtt ccaccctgct ctgcgcgacc agtttgccgc cttcttcgcc gacccggaca
     9001 cgctgacatt gggcgtgtgc aacggctgcc aaatggtcag caaccttgcc gaaatcatcc
     9061 ccggcacggc aggctggccg aagttcaaac gcaacctgag cgaacagttt gaagcacgcc
     9121 tgagcatggt tcacgttccg aaatcagcgt cgctgattct gaacgaaatg caaggctcca
     9181 gcctgcctgt cgtggtcagc cacggcgaag gccgcgccga cttcgcgctt cacggcggca
     9241 atatttccgc cgatttgggc attgcgctgc aatacatcga cggacaaaac caagtgaccc
     9301 aaacttatcc gctcaacccc aacggctcgc ctcaaggcat cgccggcgtt actaacgccg
     9361 acggccgcat caccatcatg atgccccacc ccgaacgcgt gtaccgtgcc gcgcaaatga
     9421 gctggaaacc ggaaggctgg acggaactgt ccggctggta ccgcctcttt gccggcgcac
     9481 gtaaagcctt gggctaaccg ccctactcaa accaatgccg tctgaagaat atttcagacg
     9541 gcgttccggc ataccatcct ttaaacggta tccgtccacc gaggaacact catgaaaatc
     9601 acccccgtca aagccctaac cgacaactac atctggatga tacagcacgg caaccatgcc
     9661 gtctgcgtcg acccttccga accctcgccc gtcttggaat tcctcgtccg caaccgcctc
     9721 atgcttgccc aaacatgggt aactcacccc catcccgacc acgagggcgg tgcggcggca
     9781 ctctggcgcg gctacatgga atcgcccgtt tacggcgaat ccgacatcga agcagcaacc
     9841 cacaccgtaa ccgccggcac ccaattcacc ttcggcgacg gacaggttac cgtttgggca
     9901 acacccggcc acacagaccg ccacaccagc taccttctcg aaacttcaga cggcatacac
     9961 gtcttttgcg gcgacaccct tttttccgcc ggctgcggac gcgtgtttac cggcacaatc
    10021 gaacagcttt acgacagctt ccaacgcttc aaccgcctgc ctgaaaacac cctgttctat
    10081 ccggcgcacg aatacaccgc cgccaacctg cgtttcgccg cccatatcga gccggacaac
    10141 gccgacattc agacggcact gaaagcggcg gcgcatacgc ctaccctgcc cgttaccctc
    10201 gcgcacgaac gccgcgtcaa tccgtttttg cgcgtcgacc tgccgcacgt cagagaccgc
    10261 gccgaggcat tgagcgggaa aacgttaaac agcagcctcg atacctttgt cgcgctgcgt
    10321 gaacttaaaa accaataccg gacgaaataa aacaacggga aaacgcagcc attcctagga
    10381 tttttattaa aatcttaaat aaaatcatac aatcatcgcc aatagacgaa aggacaccgt
    10441 tgccttataa tcaaacaaaa acaaaatata taatatagtg gattgaattt aaatcaggac
    10501 aaggcgacga agccgcagat agtacggcaa ggcgaggcaa cgctgtactg gtttttgtta
    10561 atccactata ttgttaatcc actatataaa tccagcacaa aacgggatcg gtgattcttg
    10621 tccgcaagaa tcgttgattt tctctattac acggataatc atcatgcgct tcacacacac
    10681 caccccattt tgttccgtat tgtccaccct cggtcttttt gccgtttccc ctgcttactc
    10741 atccattgtc cgcaacgatg tcgattacca atattttcgc gactttgccg aaaataaagg
    10801 cgcgttcacc gtaggtgcaa gcaatatttc catccaagac aagcaaggca aaatattagg
    10861 cagggttctc aacggcatcc ccatgcccga cttccgcgtc agcaaccgcc aaaccgccat
    10921 cgccaccctg gttcaccccc aatacgtcaa cagtgtcaaa cacaacgtcg gctacggttc
    10981 catacaattc ggcaacgaca cccaaaatcc agaagaacaa gcctatacct accgcctcgt
    11041 atcacgcaac ccgcacccgg actacgacta ccaccttccc cgcctcaaca aactggttac
    11101 cgaaatctca cctaccgcac tcagcagcgt acccttgctt ggaaacggcc agccaaaggc
    11161 caatgcctac ctcgataccg accgcttccc ctactttgta cgactcggct caggcacgca
    11221 acaagtccgc aaagcagacg gcacgcgtac acgaaccgcc ccggcatacc aatacctgac
    11281 cggcggcacg ccgctgaaag tattggggtt ccaaaaccac ggcttactcg tcggcggcag
    11341 cctgaccgac caacccctta acacctacgc aatcgccgga gacagcggtt cccccctgtt
    11401 tgccttcgac aagcatgaaa accgctgggt gcttgcgggc gtactcagca cctacgccgg
    11461 cttcgataat ttcttcaaca aatacatcgt cacgcaaccc gaattcatcc gttccaccat
    11521 ccgccaatac gaaacccggc tggatgtcgg gctgaccacc aacgaactca tatggcgcga
    11581 caacggtaat ggcaacagca ccctgcaagg gctcaacgaa cgcatcaccc tgcccattgc
    11641 aaacccttcg cttgccccac aaaacgacag caggcacatg ccgtctgaag atgccggcaa
    11701 aacgctcatc ctatccagca ggttcgacaa caaaacactg atgctggcag acaatatcaa
    11761 ccaaggcgca ggcgcattgc agttcgacag caacttcacc gtcgtcggta aaaaccacac
    11821 atggcaaggt gcaggcgtta tcgtagccga cggcaaacgc gtcttctggc aagtcagcaa
    11881 ccccaaaggc gaccggctct ccaaactggg cgcaggcacg cttatcgcca acggacaagg
    11941 catcaaccag ggcgacatca gcatcgggga aggcactgtc gtactcgccc aaaaagctgc
    12001 ttcagacggc agcaaacaag cattcaacca agtcggcatc accagcggca ggggcacggc
    12061 cgtcctcgcc gacagccagc aaatcaaacc cgaaaacctc tatttcggct tcaggggcgg
    12121 acggctcgac ctcaacggca acaaccttgc ctttacccat atccgccatg cggacggcgg
    12181 cgcgcaaatc gtcaatcaca accctgacca agccgcgaca ctgacgctga ccggcaaccc
    12241 cgtcctcagt cccgagcatg tcgagtgggt gcaatggggc aaccgtccgc aaggcaacgc
    12301 ggcggtttac gaatacatca acccgcaccg caaccgtcgg accgactact tcatactcaa
    12361 acccggcggc aacccgcgcg aatttttccc gttaaatatg aaaaactcaa caagctggca
    12421 atttatcggc aacaacaggc aacaggccgc cgaacaagtc gcccaagccg aaaatgcccg
    12481 ccccgacctg attaccttcg gcggatactt gggtgaaaac gcgcaaacgg gcaaagccgc
    12541 gccgagttac agcaaaacca atgaagcagc catagaaaaa acccgccata tcgcaaatgc
    12601 cgccgtatac ggccggcccg aataccgtta caacggcgca ctcaacctgc actatcgtcc
    12661 caaacgcacc gacagcacgc tgttgctcaa cggcggcatg aaccttaacg gggaagtctt
    12721 gattgagggc ggcaatatga ttgtgtcagg caggcccgta ccccatgcct acgaccacca
    12781 ggccaaacgc gaacccgttc ttgaaaacga atggaccgac ggcagcttca aggctgcacg
    12841 gttcaccctg cgaaaccatg cccgactgac ggcagggcgc aataccgcgc atctggacgg
    12901 cgacataacc gcatacgatc tgtccggcat cgacctcggc tttacccaag gcaaaacacc
    12961 ggaatgctac cgctcctacc atagcggcag cacccactgc acacccaacg ccgttttaaa
    13021 agccgaaaac tatcgtgcac tacctgcaac gcaagtacgc ggcgacatta cccttaacga
    13081 ccgttcagag ctccgcctgg gcaaagcaca cctgtacggc agcatccgtg ccggcaaaga
    13141 caccgcagtc cgcatggaag cagacagcaa ctggacactt tcccagtcca gccacaccgg
    13201 cgcactgacg cttgacggcg cacaaattac cctgaacccc gatttcgcca ataatacaca
    13261 caacaaccgc ttcaacacac tgaccgtcaa cggcacactt gacgggttcg gcacattccg
    13321 attcctgacc ggcatcgtcc gaaaacaaaa tgcccccccc ctcaaactgg aaggggacag
    13381 ccgcggcgca ttccaaatcc acgtcaaaaa caccggacaa gaacctcaaa caaccgaatc
    13441 gcttgcactt gtgagcctca atccgaaaca cagccaccaa gcccgattca ccctccaaaa
    13501 cggctatgcc gatttgggtg cctaccgcta catcctccgc aaaaacaaca acggatacag
    13561 cctgtacaac ccgctcaaag aggccgaact tcaaattgaa gccacgcgtg cggaacatga
    13621 gcgcaaccaa caggcataca accaattaca ggcaaccgac atcagcagac aggttcaaca
    13681 tgactctgac gcgaccaggc aggcactaca ggcctggcag aacagtcaaa ccgaacttgc
    13741 ccgcatcgac agccaagtcc aatatctgtc cgcccaattg aaacagacag acccgctgac
    13801 cggcattctg acgcgtgccc aaaacctgtg tgccgcacaa ggatacagtg ccgatatctg
    13861 ccgtcaggtt gccaaagccg ccgacacgaa cgacctgaca ctcttcgaaa ccgaactgga
    13921 tacgtatata gaacgtgtag aaatggccga atccgaactt gacaaagcac ggcaaggcgg
    13981 cgatgcgcaa gccgtcgaaa cagcccggca cgcctacctg aacgcactca accgtctgtc
    14041 ccgacaaatc cacagtttga aaaccggcgt tgccggcatc cgtatgccga acctggccga
    14101 actgatcagc cggtcggcca acaccgccgt ttccgaacag gccgcctaca ataccggccg
    14161 gcaacaggcg ggacgccgca tcgaccgcca ccttaccgat ccgcagcagc aaaacatctg
    14221 gctggaaacc ggtacgcaac aaaccgacta ccatagcggc acacaccgtc cctaccaaca
    14281 aactaccaac tatgcacata tcggcatcca aaccggcatc accgaccgtc tcagtgtcgg
    14341 tacgatttta accgatgagc gcacaaacaa ccgttttgat gaaggcgtat ccgcccgaaa
    14401 ccgcagcaac ggcgcacatc tgttcgtcaa aggggaaaac ggcgcactct ttgccgcggc
    14461 agatttaggc tacagcaaca gccgtacccg atttaccgat tatgacgggg ctgccgtccg
    14521 ccgccacgca tgggatgcag gcatcaacac cggcatcaaa atcgataccg gcatcaacct
    14581 cagaccctat gccggcatcc gtataaaccg cagcaacggc aaccggtacg tactcgacgg
    14641 cgcagagata aacagcccgg cgcaaatcca aaccacatgg catgccggca tccgtctcga
    14701 taaaaccgtc gaactgggtc aagccaagct gacccccgcc ttcagcagcg attactacca
    14761 tacccgccaa aacagcggtt ccgccctcag cgtcaacgac cgtaccttac tgcagcaagc
    14821 cgcccacggc acactgcata ccctgcaaat cgacgccgga tacaaaggct ggaacgccaa
    14881 acttcatgcc gcttacggca aagacagcaa caccgcccgc cacaaacagg caggaatcaa
    14941 aataggctac aactggtaac aagccgataa aaatgccgtc tggaacccgc gtttcagacg
    15001 gcatttgcgt taaaaatagt aaaccgttcc aaaagggagt agaatagtgc cgtttccaac
    15061 cctgcgcctg taccgtcagg cttttattat ggaccttccc agttcgtttt tactgaacac
    15121 cccatccgat tcaaacccgc aataaccatc ccgccggaat gcctccccgc acacggcggg
    15181 cggagcattt atgagcatcg aaccaacccc tccgaacctt gaaaacgacg gtatcgaaaa
    15241 cgatgtagaa cgcgtttccg ccgatttcga ccgtgtccac tccctctgcg aaatcctcga
    15301 acctgctttt gaacaaatcg aaaacggtac accgctcgaa gacgcgccgc tgcgcgacaa
    15361 gctgaccgag ctgaccgtcc tcttgagcga gctgcaccct gccgacgtgg cggcggtatt
    15421 ggaatcgcta ccgccgcgcg aacgcaatat cgtctggatt ctggtcaaac cggaagacga
    15481 cggcgaagta ttgctggaag tatccgacgc ggtgcgcgaa acgctgatcg agtcgatgga
    15541 caaagacgaa ttgttggcag cggtcgatga tttggacgcg gacgaattgg cggaactggc
    15601 agacgatttg ccgcaccaag tggtttacga agcgctacaa acccgcgatg aggaagaacg
    15661 cgcccaagtc aaagcggcaa tgtcctacga agacaaccaa gtcggtgcga ttatggactt
    15721 cgagttggtc agcatccgcg ccgatgtcgc ctgtgaagtg gtgctgcgct atctgcgccg
    15781 cttcgacagc ctgcccgacc ataccgacaa gatttttgtg gtcgatgaaa acgacgtact
    15841 gcagggcgtg ctgcccatcc gcaaactttt ggtcgccgat cccgaagact tggtggaaaa
    15901 cgtgatggcg aaagatgtcg tgcgtttccg cgccgaagat gacgtggaag aagcggcgca
    15961 ggcgtttgaa cgctacgact tggttaccgc gcccgtcgtc gatgaaaaca aaaagctcat
    16021 cggcaggatt accatcgacg agatggtgga cgtgatccgc gaagaatcgg aagcggatat
    16081 gctgaatatg gcgggtttgc aggaagagga agacctgttc gcccccgtgt gggattcggt
    16141 gaaaaaccgc tggatgtggc tcgccgtcaa cctctgcacc gccttcctcg ccagccgtgt
    16201 tatcggcgcg tttgaaggca gcatcgaaaa aatcgtcgca ctcgccgcgc tgatgcccat
    16261 cgtcgccggc ataggcggta actcgggcaa ccagacgatt accatgattg tccgcgcgat
    16321 ggcgatgggg cagctgacgg atatgcaggc ggggcgtttg ctgaaaaaag aagtcggtgt
    16381 cgccttggtc aacggcatca tttggggaac ggtcatgggc gcagtatctt ggctgcttta
    16441 cggcagcctc ggcatcgggc tggttatgat tgccgcgatg acgctcaacc tcctgctggc
    16501 ggcaaccgtc ggcgtattaa ttcccgtggt aatggaaaag ttcggacgcg atcccgcact
    16561 gggcagctcg gtgctgatta ccgccgttac cgactccggc ggcttcctga ttttcttggg
    16621 gctcgccacc ctattcctgc tttaaatgcc gtctgaaccc gcgcaaaaat gccgtctgaa
    16681 gcggaagctg cttcagacgg catttgacta tttatccttg ttgcacaaga ttattggacg
    16741 gtatgccggg gcagcccttt ggcaacgccg accacatcct ccccgaacag cgcgttgaca
    16801 tcggtttcgt caaacacata tttgctgtgg cagaaatcgc aatcgacttc gatgctgcct
    16861 tgttccacca ccacgccgcc gacttcttcc ccgcccagca tcaacagcat atcgctgact
    16921 ttgccgcgcg aacaggtgca tgaaaattca aacgtttccg gctcgaacac gcgcggcggc
    16981 gtttcgtgga acaggcggta taaaacgtgt tgcgcgtcca gtcctgccag ctcctccgcc
    17041 gtcagcgtgc gcgccagcgt actgacgtgt tcccatgcct cttcatccaa tacctcttca
    17101 ggcagacgct gcaccagcag accgcccgcc gcttcgtcgc ttgcagacag gacgatgtgc
    17161 gtatcaagct gttcggaacg tttcatatag ttcaccaaca tttgcgcgat accgtcgcct
    17221 tccaaaggca ctacaccctg ccagggttcg ccgtctttag gctgcagcgt cagcacgaat
    17281 acgccgccct cgcccaaaag atcgccgagg ctttcgtcat cggctatttc tgcggtttcg
    17341 tcccaccgcg cggtcgcacg gacggtacgg tcggaagtcg cttccgcaac cagcattttc
    17401 agccgcccct gcccctgaac ctgcacaatc agcgtgcctt cgtttttgag gttgcccgac
    17461 agcagaacac cggcagccaa cagctcgccc aaggcgcggc ggatggcggc gggatagttt
    17521 ttctgtttta caatgtgctg ccacacgttt tccagacgga cgtgcagccc gcgcacgggc
    17581 atatcgtcga agataaagcg ggtacgcaca tcggcgcggt tgatggcggt ttgattcatg
    17641 attttctctg actgattgtt cggatggcgg ctatatggtt gcggtcggcg cgaaaacaag
    17701 acggacggcg gatgcgcttc ccaaattatc aataaattat ataaaaatca acatattaac
    17761 tcaatctaac aagccgtttt ttgccaaaca gccgtttttt tatatacaat caacaagata
    17821 ttttcgactg atacagcata acatcgcacg gcggcacgat gcctcctgcg cggaaacacc
    17881 gatatggatt cttttttcaa accggcagtt tgggcggttt tgtggctgat gtttgccgtc
    17941 cgccccgccc ttgccgacga gttgaccaac ctgctcagca gccgcgagca gattctcaga
    18001 cagtttgccg aagacgaaca gcccgtttta cccatcaacc gagcccccgc ccggcgggcg
    18061 ggcaatgccg acgaactcat cggcagcgcg atggggctta acgaacagcc cgttttaccc
    18121 gtcaaccgag tccccgcccg gcgggcgggc aatgccgacg aactcatcgg caacgcgatg
    18181 gggcttaacg aacagcccgt tttacccgtc aaccgagccc ccgcccggcg ggcgggcaat
    18241 gccgacgaac tcatcggcaa cgcgatggga cttttgggta ttgcctaccg ctacggcggc
    18301 acatcggttt ctaccggttt tgactgcagc ggcttcatgc agcacatctt taaacgcgcc
    18361 atgggcatca acctgccgcg cacgtcggca gaacaggcac ggatgggtac gccggttgcc
    18421 cgaagcgaat tgcagcccgg agatatggtg tttttccgca cgctcggcgg cagccgcatt
    18481 tcccatgtcg gactttatat cggcaacaac cgcttcatcc acgcgccgcg cacggggaaa
    18541 aatatcgaaa tcaccagcct gagccacaaa tattggagcg gcaaatacgc gttcgcccgc
    18601 cgggtcaaga aaaacgaccc gtcccgcttt ctgaactgat ttcccaagga atacgcaatg
    18661 agtatgcccg aaatacccaa atggtacgac gatgacggac agatcgtgtc ctgtaccgaa
    18721 aagatcaaag tgatgtccga aaatatggcc gagctgtatc agacggcaca agacgcgttt
    18781 gaagacgcgc tgctgatggg ttgcggcgaa cgtcagttgc gcgattacct gctcgcgctg
    18841 attgaaggtt tggaaaatcc ctaccgcaaa gtctgaacac gccccggttg ctgcggcacg
    18901 gtttatccgt gccgtttttg cgtttgtgcg cggcttcggc ttttcagacg gcatatttga
    18961 cgttatgatt aaacagttaa caagatttat cacaacgccg tcaaaagaca gacacacaac
    19021 atgaacatca tccgcgcgct cctcatcatc ctcggctgcc tcgccgccgg cgaaaccgcc
    19081 gttttcctag caggcatcaa actgcccggc agcatcgtcg gcatgggcgt gctgtttgcg
    19141 cttttgcagg caggttgggt caaaacgtct tggctacaac agcttaccga cgcgctgatg
    19201 gcgaatctga cgctgtttct cgtgccgccc tgcgtggcgg tcatcagcta tttggatttg
    19261 attgccgacg attggttttc gatactggtt tccgcctccg ccagcacttt gtgcgtactg
    19321 ctggttacag gcaaggttca ccgctggata cggagcatta tctgatgaac gaaatcctca
    19381 ggcagcccag cgttctgctt ttcctcacgc ttgccgtgta cgcgcttgcg attatcgtgc
    19441 gcacgcgcac gggcaatatc ttctgcaacc ccgtactcgt cagcactatc gtgctgattg
    19501 cctacctgaa aatcctcgac atcgattatg cggtgtacca caacgccgcg caattcattg
    19561 atttttggct aaaacccgcc gtcgtcgtgc ttgccgtgcc gctctaccaa aaccgccgta
    19621 aaatcttcaa ccagtggctg cccgtcatcg tttcacagct tgcgggcagc gttacgggca
    19681 ttgttacggg gatgtatttt gccaaatggc tgggcgcgga acgcgaagtc gtcctctcgc
    19741 tcgcgtccaa atctgttacc aaccccatcg ccattgaaat cacccgctcc atcggcggca
    19801 ttcccgccat taccgccgcc accgtcatca ttgccggtct ggtcggacag attgccggtt
    19861 acaaaatgct gaagaacaca gtcgtcatgc cctcgtccgt gggtatgtcg ctcggcacgg
    19921 cttcgcacgc gatggggatt gccgcctcgc tcgaacgcag ccgccgtatg gcggcatacg
    19981 cggggctggg gctgacgttc aacggcgtac tgaccgcgct gattgcgccg ctgctcatcc
    20041 ccgttttggg attttgaacc cgtttcagac ggcatttcag cccatgctgt ctgaacgccg
    20101 acacactcgc aaggagaacc gttatggctg tcaacctgac cgaaaaaacc gccgaacaac
    20161 tgcccgacat cgacggcatt gccctctaca ccgcccaagc aggcgtgaag aagcccgggc
    20221 ataccgacct gacactgatt gccgtagccg ccggcagcac cgtcggtgca gtcttcacga
    20281 ccaaccgttt ctgtgccgcg cccgtccaca tcgccaaatc gcaccttttc aacgaagacg
    20341 gcgtgcgcgc cctcatcatc aacacgggca acgccaacgc gggtacgggc gcacagggca
    20401 gaatcgatgc tttggcagtg tgtgccgccg ccgcccggca aatcggctgc aaaccgaacc
    20461 aggtgctgcc cttctccacc ggcgtgattc tcgaaccgct gcccgcagac aaaatcatcg
    20521 ccgcgctgcc caaaatgcag cctgccttct ggaacgaagc ggcacgcgcc atcatgacca
    20581 ccgacaccgt gcccaaagcc gcctcgcgcg aaggcaaggt cggcgacaaa cacaccgtcc
    20641 gcgccacggg catcgccaaa ggctcgggca tgattcatcc caatatggcg accatgctcg
    20701 gtttcatcgc caccgatgcc aaagtttccc aacccgtcct ccaactgatg acgcaggaaa
    20761 tcgccgacga aaccttcaac accatcaccg ttgacggcga caccagcacc aacgacagct
    20821 tcgtcatcat cgccaccggc aaaaacagcc aaagcgaaat cgacaacatc gccgacccgc
    20881 gttacgccca actcaaagaa ttgttgtgca gcctcgcgct cgaactcgcc caagccatcg
    20941 tccgcgacgg cgaaggtgcg accaagttca tcaccgtccg cgtcgaaaac gccaaaaccc
    21001 gcgacgaagc ccgccaagcc gcctacgccg tggcacgttc gccgctggtc aaaaccgcct
    21061 ttttcgcctc cgaccccaac ctcggcaggc tgctcgccgc catcggttat gccggcgttg
    21121 ccgacctcga taccgacctc gtggaaatgt atctcgacga tattttggtt gccgaacacg
    21181 gcggacgcgc cgcaagctac accgaagcac aagggcaggc ggtgatgtcg aaggccgaaa
    21241 tcaccgtccg catcaagctg catcgcggac aagccgccgc caccgtctat acctgcgacc
    21301 tgtcgcacgg atacgtttcc atcaacgccg attaccgttc ctgacccgac acggcttcag
    21361 acggcataca taaaatgccg tctgaaccgc cggacaacat accatgacct ccacattccc
    21421 ccgccgcctc gcccgcaaaa tccgccaaac ccgccgcctg tcgcgcaaaa gcatcgcctt
    21481 tctgttcctt ttggcaggtt cggcactcgt cgccctgacc gcgctgtttt ttgcccatct
    21541 tgccgatttt gcgctggaac tgaacgccaa actggttcaa caatacccgt ggttcgcgtg
    21601 ggtcgcgctt cctttgggtt taccgcttat tgcgtggctc acacgcaaat tcgccccctt
    21661 caccgccggc agcggcatcc cgcaggtcat cgcctcactg tcgctgccct acggcgcaca
    21721 gaaaacgcgg ctgatccgcc tcgggcagac gctgctgaag attccgctaa cctttttggg
    21781 tatgctgttc ggcgcgtcca tcggacgcga aggtccgtcc gtgcaggtcg gcgcggcagt
    21841 gatgggcgcg tggggcgcgt ggtgcaagaa acacggcttg gcattcaaag ggatgcagga
    21901 aaacgatttg atggcggcgg gcgcggcggg cggtttggca gccgcgttca acgcgccgct
    21961 ggcgggcgtg attttcgcca ttgaggaact cgggcgcggc atcatgttgc gctgggagag
    22021 gcaaattctt ttgggcgtgc tcgcctccgg tttcatacag gtcgccattc agggcaacaa
    22081 cccgtatttt tccggcttca acggcggcgt attggaacat atctttctgt gggtcgcact
    22141 gtccggcctg gtttgcggcg cggcgggcgg gctgttcgga cgtttgctct atcgcggtgc
    22201 ggcggcgttt gcaccgcgca agatacgcgg cttcatccgc aaccgtccgc tgctgctggc
    22261 ggcactgatg gggctgctgc tcgccctgct cggcacgttc taccaaggca aaacctacgg
    22321 caccggctac cacgaagccg cccaagccct gcacggcatc tacgaagccc ccttcggact
    22381 cgccgccgcc aaatggctcg ccaccgtatt cagctattgg gcaggcgttc cgggcggcat
    22441 tttcactccc tcgctgacca taggcgcggt tttgggcgag catatcgccg ccatcgccga
    22501 catatcgcag ggtgcaaaca tcatcgtcct catctgcatg gcggcatttc tggcgggcgc
    22561 gacacaatcc ccgattactt ccgccgtcgt cgtcatggaa atgacgggcg gacaaagcct
    22621 gctgttttgg atgctaattg cctgcatttt cgcctcgcag gtttcgcgcc agttttcgcc
    22681 gcgtccgttc taccacgcat cgggaatgcg cttccgccag cgcgtgcttc aagaaaccgc
    22741 cgcccaaacc ggcaatgcgc ccgcaagacc gcaaacagca aacagcaaaa cgggaatgcc
    22801 gtctgaaaat taaaacgccc ccgatcaaac gccggcagcc gccttgattt gaataccgtt
    22861 ccgccgccgc ttgaaatttc agcaacaatg ccgtctgaac gacagaatgc ggttttcaga
    22921 cggcatttcc ccatcccgat attgcctaaa caaaaccgaa gcgtttgcta taattctatt
    22981 ttttaccgca tacgcaccaa tcatgtttcc cgatttctcc caaaccctct ccaaagaccg
    23041 ccacttcctg cgttccgcct tcaaaaatcc caacaaatac ggcggtttgt ccaaaatcga
    23101 agaaaaatac cgaaaatcgc acgaaatctt tttgaagcgt ttggcagcct tgccaaaacc
    23161 cgaattcgac aacaccctgc ccgttcacga gaagctcgaa gaaatcaaaa aagccattgc
    23221 caagaatcag gtaacgatta tttgcggcga aaccggttcg ggcaaaacca cgcagttgcc
    23281 caagatttgc ttggaactcg ggcgtggggc ggcaggattg atcgggcata cccagccgcg
    23341 ccgtttggcc gcgcgctccg tagcagagcg gattgccgaa gagctgaaat ccgaaatcgg
    23401 cagcgcggtc ggctataaag tacgcttcac cgaccacacc tcgcgcgatg cctgcgtcaa
    23461 gctgatgacc gacggcatcc tgctggcgga aacgcagacc gaccgttatc tcgccgccta
    23521 cgacacgatt atcatcgacg aagcgcacga gcgcagcctg aacatcgact tccttttggg
    23581 ctatttgaaa caactcctgc cgcgccgccc cgatttgaaa gtcatcatca cctcggcaac
    23641 gatagacgca gaacgcttct cccgacactt caacggcgcg cccgttttag aagtgagcgg
    23701 acggacgtat cccgtcgaaa tcctctaccg accgctgacc ggcaaagacg aagacgacgc
    23761 agaagtggag ttgaccgacg cgattgtcga tgcggcggac gaattagcgc gacacggcga
    23821 aggcgatatt ttggtattcc tgccgggcga gcgcgaaatc cgcgaaactg ccgaagccct
    23881 gcgcaaatcc acgctgcgcc gcaacgacga aatcctgccc ctgttcgcac gcctgtcgca
    23941 cgccgagcag cacaaaatct tccacccctc aggcgcgaaa cgccgcatcg tattggcaac
    24001 caacgtcgcc gaaacctcgc ttaccgtgcc gggcatcaaa tacgtcatcg acaccggcct
    24061 cgcgcgtgtt aaacgctatt ccgcacgggc gaaagtggag cagcttcata tcgaaaaaat
    24121 ctcccaagcc gccgcccgcc aacgatccgg ccgctgcgga cgcgtctccg caggcgtgtg
    24181 tatccgactg ttttcagaag aagattttaa cagccgcccc gaatttaccg accccgaaat
    24241 cgtccgcagc aacctcgccg ccgtcatcct gcgcatggca gcattgaaac tcggcgatgt
    24301 ggcggcattc ccgtttttag aaatgcccga ttcacggtat atcaatgacg gttttcaggt
    24361 gttgttggag ttgggggcgg tggaggccgt ctgaaaacag gcagacataa aagaaaatcc
    24421 gcgtagagtg atgtaaactt acccttgctt taataagtag aaaatggtgg gtttacgtcc
    24481 ccccctgcgg ctactaaaaa aatataagag taaacaacct ttttgaaaga aaaatgtatg
    24541 gacgaaattc aaatacccaa aaaagtggaa ttacaaacca aactagaaaa tgaaaagatt
    24601 gttttatcga aaggttctac cacgattatt gttggtgcta atggcacagg gaaaacaaga
    24661 ttagctgttt atattgaaga acaattaaag gaaaaagcac acagaatttc ggctcataga
    24721 gcattaaaat taaaccctaa tgtcaataaa ataccagaaa agagtgccaa aacatatcta
    24781 tcttatggtc agaactggga tggaatcgat gtatcaaata gaaaaaatta tagatgggat
    24841 aataactcat atactcattt actcaacgat tttgattggt tattacaata tttattcgct
    24901 caacaaaata atattgcggt agcaaataat caaaagctca accgtaatga aaaagtaacc
    24961 aattcaaaaa caaagctaga tattttgcaa gaagcatggg aaacattatt accacacaga
    25021 aaattacata ttacagcaga tgatattcaa gtctctgctg tagataatga ggaattgtat
    25081 tctgcctcaa atatgagtga tggagagcga gcacttttct atattcttgg acaagttttg
    25141 tcagtagatg acggttctgt cttaattttt gatgagcctg aattacatat tcataaatca
    25201 attatttcaa atctatggga taaaattgaa gaattacgac ctgattgttc atttctaatc
    25261 attacacacg atattgaatt tgctgcaact cgagtagcta aaaaatatgt tatcagaaat
    25321 tattatccga cccctgcttg ggatatttct gaagttcctg aaagtaattt tgatgaagaa
    25381 acaataacga tgattttagg tagccgtaag ccaatattat ttgttgaggg caacaataat
    25441 agtttagata ttgctactta ccgctattgt tatcctgatt ggaccatcat acccaaaggg
    25501 gcatgcaaag atgtcattca atcagtatca tcgctgaaaa aattaagtaa tgaaatgcca
    25561 ttactaaact taaaatgttc aggtattgtc gatttagata gtagggatga aagagaaatt
    25621 gaacaattaa ataatttggg tatttacatt ttacctgtat ccgaaattga aaatcttttt
    25681 agcttaactg atgtagcaaa agagatattg aaactaaatc aatattcaga tgaagaatta
    25741 ctcaataaac ttaatggatt taaatccgaa ctaattaaat atatagataa tgaattaaaa
    25801 gacgataaat tagacgaatt tgttgtaaaa caggttcgac gtaaaattga taattattta
    25861 aaaaatattg atttatcctc caaaataaca agtactgata tgaaaaaatc attacttaat
    25921 gaaatttcta ctttaacaga acagaaaatt gaaacatgga tttcagaaat taaaaatgaa
    25981 attcaaagat gtattgaaca gcaagatttg gataaattac ttactatata tgataataaa
    26041 ggactcttgg ctaaatcagc ttgtgtttta aaaggaatgc gtaacaaaca tgaatttgaa
    26101 agctggataa tgagaacatt aaaaggaagg aataaagatt ttattgatgc aatcagacag
    26161 aaacttccaa ttctggatta aataaaacca tctgaaaatt taccttcaga tacagatata
    26221 tttcatgaaa aatcatcaaa ctacactctc tttccctact tcgagtagcc tgaaaccttg
    26281 cgcagacaaa caaggcctgt ctgaagaccg cagccaatac cgcctgacca aactcggcga
    26341 acaaatggcg cacctgccta tcgacccgaa aattgcgcgt attttgttag tattattccg
    26401 tttttaaaaa tgcccgattc gcggtatatc aatgacggtt ttcaggtatt gctggaattg
    26461 ggggcggtgg aggccgtctg aaaataaaat ctttctttat aaaaaggcag gccatgtttc
    26521 attttcagac ggcctaaatc attgagaaac taaaaactat taaaaaggga atattgggtt
    26581 ttaaaactca atcggtaaat ttttattgtg aaatattaat gatgaaaaaa tctttcctta
    26641 cgcttgttct gtattcgtct ttacttaccg ccagcgaaat tgcctatcgc tttgtatttg
    26701 ggattgaaac cttaccggcg gcaaaaattg cggaaacgtt tgcgctgaca tttgtgattg
    26761 ctgcgctgta tctgtttgcg cgttataagg tgacgcgttt gttgattgcg gtgttttttg
    26821 cgttcagcat tattgccaac aatgtgcatt acgcggttta tcaaagctgg atgacgggca
    26881 tcaattattg gctgatgctg aaagaggtta ccgaagtcgg cagcgcgggt gcgtcgatgt
    26941 tggataagtt gtggctgcct gtgttgtggg gcgtgttgga agtcatgttg ttttgcagcc
    27001 ttgccaagtt ccgccgtaag acgcattttt ctgccgatat actgtttgcc ttcctaatgc
    27061 tgatgatttt cgtgcgttcg ttcgacacga aacaagagca cggtatttcg cccaaaccga
    27121 catacagccg catcaaagcc aattatttca gcttcggtta ttttgtcgga cgcgtgttgc
    27181 cgtatcagtt gtttgattta agcaggattc ccgcctttaa gcagcctgct ccaagcaaaa
    27241 tcgggcaggg cagtgttcaa aatatcgtcc tgattatggg cgaaagcgaa agcgcggcgc
    27301 atttgaagct gtttggctac ggacgcgaaa cttcgccgtt tttaacccgg ctgtcgcaag
    27361 ccgattttaa gccgattgtg aaacaaagtt attccgcagg ctttatgact gcagtgtccc
    27421 tgcccagttt tttcaatgcg ataccgcacg ccaacggctt ggaacaaatc agcggcggcg
    27481 ataccaatat gttccgcctc gccaaagagc agggctatga aacgtatttt tacagcgcgc
    27541 aggcggaaaa cgagatggcg attttgaact taatcggtaa gaaatggata gaccatctga
    27601 ttcagccgac gcaacttggc tacggcaacg gcgacaatat gcccgatgag aagctgctgc
    27661 cgttgttcga caaaatcaat ttgcagcagg gcaagcattt tatcgtgttg caccaacgcg
    27721 gttcgcacgc cccatacggc gcattgttgc agcctcaaga taaagtattc ggcgaagccg
    27781 atattgtgga taagtacgac aacaccatcc acaaaaccga ccaaatgatt caaaccgtat
    27841 tcgagcagct gcaaaagcag cctgacggca actggctgtt tgcctatacc tccgatcatg
    27901 gccagtatgt tcgccaagat atctacaatc aaggcacggt gcagcccgac agctatctcg
    27961 tgccgctagt gttgtacagc ccggataagg ccgtgcaaca ggctgccaac caggcttttg
    28021 cgccttgcga gattgccttc catcagcagc tttcaacgtt cctgattcac acgttgggct
    28081 acgatatgcc ggtttcaggt tgtcgcgaag gctcggtaac gggcaacctg attacgggtg
    28141 atgcaggcag cttgaacatt cgcgacggca aggcggaata tgtttatccg caatgagtgg
    28201 cgtaaaaacc aataaagaca aatttagatg atgtcgggga agatgcccga ccgacaagac
    28261 tatgcaaaat atgaaaaacc aagtacgcgg atcaggcatg gatgcccgat ccaatccggc
    28321 caatgtttca gacggcctgc aaaacagttc gggtcatatc ggtaccaaca cgcgttaccg
    28381 cctgaccaaa ctcggcgaac agatagcgcg cctacccatc gacccgaaaa tcgcgcgcat
    28441 tttgctggcg gcgaagaaac acgactgcat ggcggaaata ttggtgattg cgtccgcgct
    28501 gtcgattcaa gacccgcgcg agcggccgct agaagcgcgc gatgcctcag ccaaggcgca
    28561 cgagcgtttt accgacaagc agtccgattt ccttgcctat ctgaacattt gggacagctt
    28621 ccagcgcgaa cgcgataaag gcttgtccaa caagcagctg gtgcagtggt gccgccaata
    28681 tttcctgtcg cacctgcgga tgcgcgagtg gcgcgagctg caccaccagc ttgcccaaac
    28741 cgcgattgaa atgggtttaa ccaccaagga agccgctttc agacgacctc ccgaagtcag
    28801 gcagctcacg tcgtctgaaa atgcgggtga ccaagaccta tctgctaaac tcaaacaaaa
    28861 acaactggat aaaaagcaac accgcgccca aatccgcgcc gccaaagaag cgggctacga
    28921 acaaatccac cgcgccctgc tcactggcct tatcgccaac gtcggcatga aatcgcccga
    28981 cggtaacgac tacaccggcg cgcgcggcag ccgcttccac cttttccccg cctccgccct
    29041 gttcaaagcc aaacccaaat gggtgatggc ggcagaattg gttgaaacca cgcgccttta
    29101 cgcgcgcgac gtcgccgtta tccagcccga atggatagag caggaagcgc cgcacctcgt
    29161 ccgctatcat tatttcgagc cgcattggga acaaaaacgc ggcgaagtcg tcgccagcga
    29221 acgcgtgacg ctttacggtc tgaccgtatt gccgcgccgc cccgtgtctt acggcaaagt
    29281 tgcccccgaa gaagcgcgcg aaatctttat ccgcagcgcg ttggtggcgc aggaatgcga
    29341 tttgaaagcg gatttttttg tccacaacaa aaagctgatt aaagaaatta ccgaactcga
    29401 acacaaatcg cgcaagcaag acgtgctggt cgatgacgaa gccctgtttg cgttttataa
    29461 cgaacgactg cccgaaatgg cttggaaaga cgcgcaaggc agcgtttggg gaagtgaaga
    29521 ttccgtacgg attattgaat ctgacaaagc cgagaggtcg tctgaaaatg agcgcaacga
    29581 gtttcgtaaa aacaagcgta atgggtctcg ccaaaatgaa aatcacggca acaccgtagg
    29641 ttgggttgaa aacccaacat cagccgcaac tgcaaaaact gttgggtttg acaatccaac
    29701 ctacgctgcc caacaaacca ccccctcccc cgtgggggag ggtcggggag agggcaaaac
    29761 agttgccgca caaaccaact tttccgcaac cgcagcaaac cctctcccta accctctccc
    29821 gcaggagagg gaacagagtg ccgcagcttc aacgatttca gacgacctgc gtcctgcaaa
    29881 tctgcagcaa accgccccct cccccgtggg ggagggctgg ggagagggca aaacagttgc
    29941 cacacaaacc aacttttccg caacctcaac aaaccctctc ccgcaggaga gggaacagag
    30001 tgcctcagct tcaacgtttt cagacgacct gcgtcctgca aatctgcagc aaccctctcc
    30061 ctcccccgtg ggggagggct ggggagaggg caaaacagtt gccacacaaa ccaacttttc
    30121 cgcaacctca acactttcag acgactccaa acccaaaaag cagcctgcac cccaaaaaaa
    30181 ccgtctgaaa cccctacccc tcgccgacat ccgcaccttc caagcctggc tcaaaaccgc
    30241 cgagcgcgac aatccgcgcc tgctgttcct cagccgcgac gatctgatgc aacacgccgc
    30301 cgcacacatt accgaagaac agttccccaa attctggcaa accgcagacg gcaaattcaa
    30361 actttcctac cgcttcgagc cgcaccatcc gctagacggc gtgaccatga ccgtgccgct
    30421 gaccgtcctc aaccgcctgc acgcgccgtc gctcgaatgg ctggtgcccg gcatgatacg
    30481 cgaaaaaatc cagttgcaaa tcaaagcact gcccaagcaa atccgccgca tctgcgtgcc
    30541 cgtgcccgaa ttcatcaccc aatttttaag ccaaaacccc gaccgcaacg cccccatcct
    30601 gccccaactc gcccaagcca tcgccaaaac cgcaggcgac atccgcatat tcgagcaaat
    30661 caaccaagac gaatgggccg cgttcaggct gcccgaacac tgctatttca acctccgcat
    30721 tatcgacgac ggcggacaag agcttgccgg cggccgcaaa ctgcacgaat tgcaacaaca
    30781 actcggtcaa gctgccgccg ttaccttccg tgacaacacc caagaatttg agcgcgacaa
    30841 cgtcaccgca tgggacatcg gcaccctgcc cgaatccatc aaattcgcac gcggcaaaca
    30901 acagctcacc ggctatctcg gcctacaaaa agaaaaagac ggccgcatcg ccctgcgcct
    30961 gtttgatacc acagaagccg cagagcaggc acaccgtcaa ggtgtcatcg aattgatgaa
    31021 gctgcaatta aaagagcagg taaaggattt gaacaaaggc atccaaggct tcacccaagc
    31081 tgccatgctg ctcaaacaca tcaacgccga cactctgcgc gacgacctca cccaagccgt
    31141 ctgcgaccgc gcctttatcg gcgaagacga gctgccgcgc aacgaaaaag ccttcaaaga
    31201 acaaatcaaa cgcgcccgca gccgcctgcc cgccgtcaaa gaagccctca gccgctacct
    31261 gcaggaaacc gccgccgtct acgccgaact caacagcaaa ctcggcaaac acccattgac
    31321 ccaccttcta agactacgcc tgcaaaccct gctcgccgcc ggcttcgcca cccgaacccc
    31381 gtgggcacaa tggccgcgcc tccccatcta cctcaaagcc atgaccctgc gcctcgaaaa
    31441 atacagcagc aaccccgccc gcgacgcagc ccgcgaagcc gatatccaag agctggaaca
    31501 aatgtggcag gaaaaaacag acagcctgat taaacaaggt ctccccattt cagacggcct
    31561 cgccgcgttt aaatggatga ttgaagaatt gagggtgtcg ctgttcgcgc aggaattgaa
    31621 gacaccgtat ccggtgtcgg tgaagcggct gttgaaagag tgggaaaaaa ttgaaaaata
    31681 aaaaaacagc ctgaaaagtt tcaggctgtt tttttatttg actaatcgaa gtttcctata
    31741 tctatttaag tccctctcaa ctaatccaaa agttaaatca gcaacatctt tgggggatac
    31801 gtttaaattt tcagcaatct gttcaatacc aatgccatca ttttttaaaa tagtaagcat
    31861 tttacgtaat gcgcttgata tttccctttc cattggctct ggttcgatag ttcgatattt
    31921 tttctttgca aacaaaggac aaagattgtg tatatacatc ctatcagtaa tcattcctaa
    31981 tttatgcatc cgatatgcta aggcaacaag tgatacacca aatcgtcttt tgatttttaa
    32041 taaattttca atagtgatag gaacatgacg atataagcgt agtgcagcct ccggcattaa
    32101 aaaagctgaa gcaaaggcat tagcctcttt ttcgataata tcacgaggtt catcttctgt
    32161 aatttcacta tttttactat gttccatact gtatttatca cggattaagt gccctaattc
    32221 atgggcagca tcaaatcgac tacgttctgc agatttttgt gtatttaaaa atacaaatgg
    32281 atgattttca taccaagtac aaaaggcatc aatgtccttt gtatctaaag ataatgaaaa
    32341 tacacgaaca cccttaactt caagtagggt gatcatattc ggaataggtt cattgccaag
    32401 cccccattct aatcttagtt cctgagcagc ctcttcagga gaaatatcag aaaaatcagg
    32461 caatacggct tgacttagtg taaattctgt ctcgagccag tcatttaaca aaaaagccgt
    32521 aatgctatga tttaatgctt gtttttcaag cctcttcgag gtgcgtgaac gagcacgaaa
    32581 acttactgcc tgagatttca actcaggcag tctttcgtca ttagtaaaga aatgaactgg
    32641 aaactctaat aaattggcta attcatttaa atcaggtatt tgctcatctt ttacatagtt
    32701 tctaacctgt cgagcggtaa tacctaataa ctcagctaat tttgtttgcg tacaaccacg
    32761 tttatccagc gcaaattcca gtctctcacg attaaatgtc tgcatgattt atcattcaaa
    32821 ttatcctgct ttttgttttc tttcaaccaa aggctcatat tcttcaacag gttgcttacg
    32881 ttcaagctca tcaaatttag ttaaatcaac atcagctaat ataattcgct gcttgtaccc
    32941 agttatttga tgactaacaa aaccactcgg taaagataat tcaagttgca ctttattata
    33001 cttccagtga aacagcagaa cccaaaactg cacagtatca ggcaaatcta gttttgaatt
    33061 acgaatagcc tcctcaaatc ccttaccttt ccttgcggtt gtcattggca tcccgtgatg
    33121 cctaccaaca tctgaagtag cagtagccac aataatactt ttagttcgac atggcgagag
    33181 acatagaaat gcaccacccg accaaggctc aagcgtccag ccatctttac ttaaatatac
    33241 ccttagagca aatgtaattt ctgcttgccg atacatcccc aatgtatttc tgtcagataa
    33301 tgctgctttg tcctgaatat tattatgcgc agtaagtaca atctctttaa gcatctcctg
    33361 agataagtac ttgctgattt cacttaaagc aatatcacta ttttgttgct cgactatttc
    33421 tcctacttca aatgggaaag gttctgataa tgcaaattcc accataaaaa tttcctaatt
    33481 ttatacgtaa tgtttacaca atatatcagg aaatatgaaa acgtacaact atatctataa
    33541 agcaattaat aagtagcctg cccaaccgtg tccttatctt tcggcacacc cgacctgcaa
    33601 atcacgcaaa acttggaatc cgtgtgtagg gtgtgtgcgg tacatacgca cgcagtcttt
    33661 ttaaaccaca gcccttccca actaaaccaa aaggtcgtct gaaccctatt ttcagacgac
    33721 cttttgccac tttgtaaaac aaatcttccc accatcctct ccccaaacat cgcccgaacc
    33781 agtaaacttc tcatatttca acaactcctt ggaagcaaac catgtctggt atctacctac
    33841 cccgcctatt cccgccccat atcgccgaac gcggcctgtt gtattttcag cagggcaagg
    33901 ttctcgatgt ccgaaaaact tccgccgggc attatcgggc ggaggtgtgc ggttcggaaa
    33961 actattgggt atagttgaag ctggatagtg atttgtatat taaagacgaa ggctgcaatt
    34021 gtccttatat ctaagagtgc aaacatacct taaattacta tattgcatag gcaaaataca
    34081 agcctataac gaattggaaa caaaatgccg tctgaaaaca tcttcagacg gcattataaa
    34141 atctgttcac cttttcagat gagtaatgta cacccttata caatttttgc tactatgccc
    34201 cataaatcca cggctaaaga tatccttatt atgtcctatg atttatcgaa acgacttgta
    34261 atcggcttag catcaagtgc cctattcgac ttatccgaat cggataatat atttagaatg
    34321 gaaggggcag aaacctatag gcaatatcag agagaaaaac aaaaccatcc cctaaaaaaa
    34381 ggcgttgtct ttccatttat taaaaaactt ctgtcaatca atgaaataaa cccaaacgac
    34441 ccaacgattg ggtttattct tttatccaga aacaatccag atacagatta cgagtcataa
    34501 ctataggctt aatattacac gattctcatt ccatcaaggc ggaaaaccgc acaaatactg
    34561 aaacactatc gatcgatttg taaacaagcc tacttaagta acttgcagtc cttatcattt
    34621 cctttaaaat aatccagccc gtcactacac gaactggcgg acttcttgca aataaaggtt
    34681 actagatttt cattcatctt aataataaaa ggatttttat ctttatctat ggctaccgcc
    34741 ttcaacatga atttactgtc taaagccccg cgcgcgattc cattcaaacg gatacaaaag
    34801 ccttctgcct ctttaatcgg caaacttggc cacttggtag atgtttgttt aaacctccca
    34861 ttctgcagat aaaacttttc cataaaatgt gcattttcta acaaggctgc ccgcactgca
    34921 tttatctttg ctttctcaac ataattgcga tagctcggat aaacaattaa agcaagtaca
    34981 gacaatatca agaccactga tattaattca accagcgtaa acccccgatt atcagtcatt
    35041 actttacttc caataagaac agattattca acatatttct ttgaacagac ttactatccc
    35101 attcaacagt atgcatattt cccactctat tttttagcgg ccggtatagc cggtttggct
    35161 gggccttttg gtgcgggcgc gccgaccgaa gcctggtcct tcagcttcgc cagcaccgca
    35221 gggccgatgc cctttacctt ggtcaaatcg tctacagact tgaacgcacc gttttgcgca
    35281 cggtattccg caatggcctt cgccttcgc
//
LOCUS       DAWIVB010000004        31903 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWIVB010000004 DAWIVB010000000
VERSION     DAWIVB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31903
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.004"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(177..824)
                     /locus_tag="WIX60_000116"
     CDS             complement(177..824)
                     /locus_tag="WIX60_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ5304450.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(821..1189)
                     /locus_tag="WIX60_000117"
     CDS             complement(821..1189)
                     /locus_tag="WIX60_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ5304451.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1199..1870)
                     /locus_tag="WIX60_000118"
     CDS             complement(1199..1870)
                     /locus_tag="WIX60_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ5304452.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2035..2754)
                     /locus_tag="WIX60_000119"
     CDS             complement(2035..2754)
                     /locus_tag="WIX60_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ5304453.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2837..4150)
                     /locus_tag="WIX60_000120"
     CDS             complement(2837..4150)
                     /locus_tag="WIX60_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ5304454.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(4548..5420)
                     /gene="hpnD"
                     /locus_tag="WIX60_000121"
     CDS             complement(4548..5420)
                     /gene="hpnD"
                     /locus_tag="WIX60_000121"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ5304455.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5591..7453
                     /gene="hscA"
                     /locus_tag="WIX60_000122"
     CDS             5591..7453
                     /gene="hscA"
                     /locus_tag="WIX60_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ5304456.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7530..8186
                     /locus_tag="WIX60_000123"
     CDS             7530..8186
                     /locus_tag="WIX60_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ5304457.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ
                     "
     gene            8223..8984
                     /locus_tag="WIX60_000124"
     CDS             8223..8984
                     /locus_tag="WIX60_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ5304458.1"
                     /translation="MMGDSVIYYVEQADEPVNRADERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQILLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNSDYLREQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASTTSEGYTALDFACLTGWQNVADLLEPRH"
     gene            9039..9380
                     /gene="fdx"
                     /locus_tag="WIX60_000125"
     CDS             9039..9380
                     /gene="fdx"
                     /locus_tag="WIX60_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ5304459.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9803..10357)
                     /locus_tag="WIX60_000126"
     CDS             complement(9803..10357)
                     /locus_tag="WIX60_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ5304460.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRDLIALDKAAAGCDSAMLRAQRWLIYTQIAERLAGSGGGFT
                     VTVESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEARVERYPAYCPTVALKV"
     gene            10570..10767
                     /gene="iscX"
                     /locus_tag="WIX60_000127"
     CDS             10570..10767
                     /gene="iscX"
                     /locus_tag="WIX60_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ5304461.1"
                     /translation="MKWTDTQRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10835..11236)
                     /locus_tag="WIX60_000128"
     CDS             complement(10835..11236)
                     /locus_tag="WIX60_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ5304462.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            11448..12407
                     /locus_tag="WIX60_000129"
     CDS             11448..12407
                     /locus_tag="WIX60_000129"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ5304463.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12475..13785
                     /gene="tilS"
                     /locus_tag="WIX60_000130"
     CDS             12475..13785
                     /gene="tilS"
                     /locus_tag="WIX60_000130"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ5304464.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAASDTLAMGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            13859..14644
                     /locus_tag="WIX60_000131"
     CDS             13859..14644
                     /locus_tag="WIX60_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ5304465.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGFFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVILGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLVRYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            14654..14794
                     /locus_tag="WIX60_000132"
     CDS             14654..14794
                     /locus_tag="WIX60_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304466.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LI"
     gene            14779..14931
                     /locus_tag="WIX60_000133"
     CDS             14779..14931
                     /locus_tag="WIX60_000133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304467.1"
                     /translation="METHLIVQCRGGGGFGLGVVEMVLTDIAQNRANGIRTNKEFGKK
                     FVKSTN"
     gene            15121..15465
                     /locus_tag="WIX60_000134"
     CDS             15121..15465
                     /locus_tag="WIX60_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304468.1"
                     /translation="MRIFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLSCYY
                     DEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKELDEV
                     LLKYIPFFEEER"
     gene            15477..15782
                     /locus_tag="WIX60_000135"
     CDS             15477..15782
                     /locus_tag="WIX60_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304469.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSAAGIKPH"
     gene            15844..17220
                     /gene="mpl"
                     /locus_tag="WIX60_000136"
     CDS             15844..17220
                     /gene="mpl"
                     /locus_tag="WIX60_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ5304470.1"
                     /translation="MKHIHIIGIGGTFMGGLAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIDVYEGFDAAQLDEFKADVYVIGNVAKRGMDVVEAILNLGLPYISGPQWLSENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLNVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            17427..18479
                     /gene="bioB"
                     /locus_tag="WIX60_000137"
     CDS             17427..18479
                     /gene="bioB"
                     /locus_tag="WIX60_000137"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ5304471.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(18557..19192)
                     /locus_tag="WIX60_000138"
     CDS             complement(18557..19192)
                     /locus_tag="WIX60_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ5304472.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQVGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            19515..19775
                     /locus_tag="WIX60_000139"
     CDS             19515..19775
                     /locus_tag="WIX60_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ5304473.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19838..21697)
                     /gene="ilvD"
                     /locus_tag="WIX60_000140"
     CDS             complement(19838..21697)
                     /gene="ilvD"
                     /locus_tag="WIX60_000140"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ5304474.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEQWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21870..23639)
                     /gene="cysI"
                     /locus_tag="WIX60_000141"
     CDS             complement(21870..23639)
                     /gene="cysI"
                     /locus_tag="WIX60_000141"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ5304475.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(23666..25480)
                     /locus_tag="WIX60_000142"
     CDS             complement(23666..25480)
                     /locus_tag="WIX60_000142"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ5304476.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKAAPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFIRLLRPLAPRL
                     YSISSAQAEVGDEVHLTVGVVRFEHEGRARTGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25477..26763)
                     /locus_tag="WIX60_000143"
     CDS             complement(25477..26763)
                     /locus_tag="WIX60_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ5304477.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPMSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26802..27725)
                     /gene="cysD"
                     /locus_tag="WIX60_000144"
     CDS             complement(26802..27725)
                     /gene="cysD"
                     /locus_tag="WIX60_000144"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ5304478.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(27763..28503)
                     /locus_tag="WIX60_000145"
     CDS             complement(27763..28503)
                     /locus_tag="WIX60_000145"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ5304479.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKTEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(28514..29965)
                     /gene="cobA"
                     /locus_tag="WIX60_000146"
     CDS             complement(28514..29965)
                     /gene="cobA"
                     /locus_tag="WIX60_000146"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ5304480.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGE
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQTYPALGGLNAGQRAA"
     gene            complement(30091..30249)
                     /locus_tag="WIX60_000147"
     CDS             complement(30091..30249)
                     /locus_tag="WIX60_000147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304481.1"
                     /translation="MPSEALFALRTASPHHANGLNARNGFRPFFFRNRYKTENRRIRS
                     RPHIAFSF"
     gene            complement(30262..30918)
                     /locus_tag="WIX60_000148"
     CDS             complement(30262..30918)
                     /locus_tag="WIX60_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ5304482.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFVLFLLVFRLCWGIWGSDTARFSRFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLILPMITGFKYIEGKTSIRFAGKAALAAALSVASLAAAAILLLS
                     "
     gene            30982..31872
                     /locus_tag="WIX60_000149"
     CDS             30982..31872
                     /locus_tag="WIX60_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ5304483.1"
                     /translation="MFPVFHLSGESRRQMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDTLPFAVIAGFPEIMLDTADSLAKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKPVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITKSM"
BASE COUNT         6876 a         8383 c         9259 g         7385 t
ORIGIN      
        1 acaaccacgc cggaattaag tttaaatttg aataaaaggt tcgggttctg caaaatacag
       61 aacccgaacc ttgttcggat attgaaaccg gctgcccgat tttgggcggt gcggcttgca
      121 agtatcaaga ttcgcatatg ccgtctgaag ctcggagagg ttcagacggc atatgcttat
      181 ttgggctgct cttcaacgaa tctcggacct ttcaagatgc cgttgtgaga atagggcgac
      241 agcaggttgt atgcggcggt tttggaaacc tgataaccgc ggtcggtcag gctgttggca
      301 atctgattga ccactgcgct gaccaaagcc cccaacaggc cgctgttgct gttgttgctg
      361 ccttcgcgga tgctggccga acccgaccac aactcttttc cgttgcggga atcgaccagc
      421 cgtgctttgg cggatacggt cgtcacgctg tctaaaattt gatatgaagt gccgtattcg
      481 gtaaccgtaa tgtacaaaac cgcatcattg ccgaaaatct gatgcagttt ttccggccgg
      541 acggcgtgaa tatcggcggc attggtcaag ccgttttgtt tgaaggtttc ctccacgact
      601 gcggcgggga agacgtaata gccggcttcg gaaagcggcg cggcggtcga agccagtaca
      661 ccccatgttc cgttgacatc gggcgattcg ttcagcggcg gaaccaccaa aattgaagcc
      721 ggtttgcttt ccttgaatga cgtgtagtcg aaatcgggcg ctttttgaac ttggcaggca
      781 gacagcgcca acacggcggc aagccctaaa atcaaaggtt tcatcgcttg cctcctttac
      841 cggttttcat caggaagtcc ataaatacgc ccgattcggg aaacagcctt ttctcttctt
      901 caaactggcg gaacgcgccc tctttgtctc ccgaacggga aagcagcagt cccagatggg
      961 cgtgcgcacc cggggcggca ttcatttttt tgttgccggc ttccacaaag tatttttcca
     1021 tcttttcggt ctgcttgccc aacgaagtgt cgtcgttttt caaaccttca tagacggtat
     1081 cgggatagcc gccgtaataa tacagggatt tttgcccgtt gccgccgcag gcggtcagag
     1141 ccaagaccgc cgcacacagc gacaaacggc tcaaggtttt cggattcatc atttctcctt
     1201 aacggttggg ttgccatgcg ccgttgtcaa cagcctgaac caggctgttg acggcttcgc
     1261 ggattgccaa gtctaaaact ttgccgttca aagtcgcatc gtagccggaa gtgccgccga
     1321 aaccgatgat ttcacggttg gaaagtgcgt attcgcccgc gccctgtgcg gaatagacga
     1381 tttcggaagt attgacgttg acgatattca gagccacttt tgcataggcg atttgcgatt
     1441 tgccgcgacc caaaatgccg aagagctgat gatcgccgac atctctgcgt ccgaattcgg
     1501 ttacatcgcc ggtaacgaca taatctgcgc ctttcaggtt atgcgctttg ccggaaatgc
     1561 cggattcctg ttttaatgcg ttcaaattgg tgcggttcag tacgttgaag cggttggtct
     1621 gttgcaggtg cgttactaga atggtttttg cctggctgcc caaacggtct tccccgtcgg
     1681 agaaaatgcc tttttggaag ctggagcggt tgtcgaatgt tccgacggaa atcggggtac
     1741 gaacaccgtg atattgcgta ttgtaggagg cgactttctc tacctcgaga ctgcgtgagg
     1801 attcggtcgc acagccggtc agtgaaacgg cagcggcggc aaggacaacg gcggtggaaa
     1861 cggttttcat aaaatttacc ctaaggtcaa gttaaggaaa taacgggttg tcattattgt
     1921 ccttatgtaa atttaagtca aggtgtttgt ctgtgcggga cggatgcgcg cggaaggtac
     1981 ggatattttt caaacggaat cgcggcgcgg gcgggatgcc tgccttttcc ggatttagag
     2041 gtaaacgatt tcgccactcc gccctttgct ttcggcactt gcccaccaga caaatgcggg
     2101 cagcacgtcc ccgtagcttt tgcgttcgct tttggcttcg cccggatggg atttgatgcg
     2161 ttgcggggaa ttgatggggc cggggacgag gacgttggcg cgcaggttgc cgaagcgttc
     2221 ccattcgtcg gcggcgactt tgcacaggta gttcaacgcg gctttggacg cgccgaagcc
     2281 gccccagtag gctttgggtg tttcgccgtg gctttcgccg acgaagatga cggacgcgtc
     2341 gggcgactgc ttcagcagcg ggaacagggc gcgggtcagc cccataggtg cgacggtgtt
     2401 gatgcggtat tggttgaccc attcggcgac ggtttggaaa tccagcggcg agagggcgta
     2461 aaaatagccg gcgcagtgga cgatgccgtc cagtttgcct tgcgtggctt cggcaatggt
     2521 ggcggcgaaa tgttcaaatt ctttttcttc cgcgctaata aggtcaaagc agatggcgaa
     2581 tggttcgggg tatccggctt cgacaatcgc gtcatacact ttttccagtt ttttctgatg
     2641 acgggcaacc aaaatcacgg ttgcgcctgc cgccgcatag gctttggcga cctgttcgcc
     2701 cagaccttgc gatgcgccgg ttactaagat ggttttgtcg gacagtgtcg ccatactttt
     2761 tcctttttgg ttgtcggtta aggtatttta gcgttttgcc gcaccttgta aagcgtcccg
     2821 atgtcggccg gcgttttcag acggcatcgc tcaggctttg caggcaggct tccgccgacg
     2881 cgaaacctga ttgtacggcg gcttcgagcg tggcggggta gtccgggtgg aggtagtcgc
     2941 cggcggggaa gatgcggtgc cggtgcaacc acgacaagtc cggcggcggg gcatcggctg
     3001 cggttgtggc gcgtttttcg gtgatgacgc gcacggcttc gggttcgccc aaatgcggaa
     3061 ggatgcgttt gaggtcggcg tgggctttgt ccgcccacgc ccggtttgca aacgcgccga
     3121 cgcggtcgga aacgctgatg acggcggaca cttcgttttc aggcagtccg agcctgcccc
     3181 ggcaaagcag ccattgcacc gtgccgtcgg caaggccggt cagcggggcg ggcaggcgga
     3241 cgggttcggc gtagcgcaga tagacggtgg tgatggcgtg gtagcgaagg ttttgatatg
     3301 ccgtctgaac gtgttcgggc gtgccttcgg gcaggagcgc ggcggcgtgg tagggcgcgg
     3361 tggcggggac ggcggcatcg aaagcttcgc cgttgacgag cactttcccg tccgggaggg
     3421 tgttcagacg gcatacgcgc gtttcgaggc ggatgtccgc gccgagccgt tgaagatccg
     3481 ccaaggcggg ttcggcgacg attgcgccca aatcctgctt gggtaggaga tagtcgctgc
     3541 cggatttttt cgtcagcacg ccgtcggaca aaacgttgca caacacgcgc aggcttgcgg
     3601 tttccaaagg cgtgttgagc gcgccccaaa ccaagggctg ccaaaactgc atcacggcgg
     3661 cacgcggcac gttccgctgt ttcagccatt gcgccactgt cgtgtcgggc tgtccgaggc
     3721 gtgcggactt ctgcaaatcg gacatatcgg caagcagttt ggctttgaat gcagtcggtg
     3781 cacgccgggc aagcagcacg ccgcccaaaa tatgcagcgg cgcgggcagg gggagggcgc
     3841 ggaactgcaa accgccgtgc atatgccagt gcagcggtac gcgcaaaaag gcggcacggg
     3901 gatccgaacc gatggttttc atcaggcgca acacgccccg gtatgcgccg agcaaaatgt
     3961 gctgcccgtt gtccaaaaaa ccgaaaccgt cggtatttcc ggccagtgtg cgcgccctgc
     4021 cgcccgcctg ccggccggct tcaaacaggg taacgtcggc gtgccgcgcc aaggtgacgg
     4081 cggcggacag tcctgcccag cctgcgccga tgacggcgat tttcgggcgc ggatgcggcg
     4141 tgttcatcat ttattcctcc aatggttttg cagccgtatc tatttccgtt tccgaaaatg
     4201 acggtagaaa aggatacagg ctaaaaataa aggcagcaga aagcgcgcat agggatacca
     4261 cggatggtct gcatgccagt atccgtaccg tccctgtgga acggtatcat agcggtaact
     4321 gtcggggaaa aagttgatcc aaaccgttac tgccagccat aaggctatat ccctgactgc
     4381 tttcatttac ttctgctcct gtttaaattc ccagcaattc catttcaaag cgcgaacgcc
     4441 aacgggattg cgcggttacg atgcagactt tcagacgatg gttgaaaccc cgtccggcgg
     4501 ggctgtggga atcgggtttg cctgacgggc ggcgggcggt atgcgcctta tgcccgttcc
     4561 ggcgtgccgg ggcgcggttt gaatccgaat aaccaggttt tcagggcaat gcgttttttg
     4621 cgcggcgaag ggagggcgat tttgtatttg aggacgtttt gtgcgccgtc tcggtcgatt
     4681 tcgttcaata gctcgtaata aaccgccgcc atgaccagtc cgactttttg ggctttttta
     4741 tcggcatcag gcagcagcga tacggcttcg cggtaggttt cgcgggcgcg tttgatttgg
     4801 aacgccatca attcggcaaa attgcccgtc gggctgcatt gcaaaatcac gcttgcgggt
     4861 acgtcaaacc gccgcatttc ctccatcggc aggtaaatcc gccccctgcg cgcgtcttcg
     4921 ccgacatcgc ggatgatgtt ggtcagttgc agcgcaagtc ccatcttgtc ggcgtattcc
     4981 agcgtttggt cgtctgaaaa ccccaaaatc cgcgcaatca ggcagccgac cacgcctgcg
     5041 acgcggtggc aatacagttt caattcttca aaactgccgt aacgggcttg aaccaaatcc
     5101 atctgcatcc cgtcgattaa ggcttccagt tcatatttcg gcagcttgaa ggtttcctta
     5161 acttgccgca aggcctgatt gacggggtgt tccggcatcg cgccgccgaa taccttgtcc
     5221 aaatcgccgc gccaccagtt caatgtcgcc tgtgcaacat cggggttgga acattcgtca
     5281 accacatcgt ccaattcgcg gcaaaaagca tataaaaccg ttaccgcatc ccgtttttcc
     5341 tgagtcagga aacggaagcc cgacaaaaaa ctggagcggc tttcttctgc tttttggcgg
     5401 caatagtcga gtcctttcac gatttatatt cctaatgatg ggcgggaaag gcggatttta
     5461 tcggcatttg gcggtagagg gcaatttcgg cggcacgacc taatccttag cggtttgctc
     5521 aactatcggc gcaaattctg ttaaaatgcc gctttccttc ctttacacac cgcaccgaca
     5581 ggcagaattt atggctcttt tgcagatttc agaaccgggt atgtccgccg ccccgcaccg
     5641 gcaccgtttg gcggcaggca tcgatttggg tacgaccaac agcttggtcg ccaccgtccg
     5701 cagcggcagt gccgcctgcc tgcccgatgc cgaagggcgc gttaccctgc cttccgtcgt
     5761 ccgctatctg gaaaacggcg gcattgaagt cggcaaaacc gccctgtccg cccaaaaaac
     5821 cgacccgctg aacaccgtca gctccgccaa acgccttatc gggcggacgc ttgccgatct
     5881 gcatcaaaat acgcactacc tgccttaccg tttcggcgac aatcaacgcg ttatcgaact
     5941 gcatacgcgg cagggggtga aaacgcctgt cgaagtgtcg gcggaaatcc tcaaaaccct
     6001 taaatcgcgc gccgaagaaa ccttgggcgg cgatttggtc ggcgtggtga ttaccgtccc
     6061 cgcctatttc gacgatgccc aacgccaggc caccaaagat gccgcgcgtc tggcgggttt
     6121 gaacgtattg cgcctgctca acgaacccac cgccgccgca atcgcctacg ggctggacaa
     6181 cgcctcggaa ggcacgtttg tcgtgtacga cttagggggc ggcacattcg acgtatccgt
     6241 attgcaactg accaaaggac tgtttgaagt caaagccacc ggcggcaaca gcgcgttggg
     6301 cggcgacgat ttcgaccacc gcctgttctg ccgcctgctc gaacaaaacg gactctccca
     6361 actcaacgaa caagacagcc aactcctgct ctcgctcgtc cgcgccgcca aagaacaatt
     6421 aaccacgcaa accgaagcgc gcattcaggc gacgctttca gacggcatgg caatcgacac
     6481 aagcatcagt cgcgccgagt tccacaacct gacgcagcat ttggtgatga aaacgctcga
     6541 accggtcaca caggcgttga aagatgccgg tgtcggtaaa aacgaagtca aaggcgtgat
     6601 tatggtcggc ggttcgaccc gtatgctgca cgtccaacag gcagtcgcca ccttcttcgg
     6661 acaaaccccg ctgaacaacc tcaaccccga cgaagtcgtc gcgctcggcg ccgccataca
     6721 ggcaaacgtc ctcgcaggca acaaaaccga cggcgaatgg ctgctgctgg acgttacgcc
     6781 cttgtcgctc ggtttggaaa cctacggcgg cttggcggaa aaaatcatcc cgcgcaattc
     6841 caccatcccc accgcgcgcg cgcaggactt taccaccttc aaagacggtc agaccgcgat
     6901 gacgatacac gtcgtacaag gcgaacgcga actggttgcc gactgccgca gccttgccaa
     6961 attcaccctg cgcggcattc cgcctatggc ggcgggtgcg gcgcgtatcc gcgtaacctt
     7021 ccaaatcgat gcggatgggc tgctgtccgt ttccgcccaa gaacaaagca ccggcgtaca
     7081 ggcgcaaatc gaagtcaaac cctcctacgg cttggacgac gacaccatca cccaaatgct
     7141 caaagacagc atgagcaatg ccgccgaaga tatggcggca cgcgcccgtg ccgaagccgt
     7201 agtcgaagcc gaaagcctga ccgatgccgt caacgccgcc ctcgagttgg acagcgattt
     7261 gctggatgcc gaagaattgc aacagattcg gcaaggcatc gccgatttgc aaggccgtct
     7321 gaaagacgga aaagccgaag acatccgtgc cgccgccgcc aaactcggca gcatcaccga
     7381 caatttcgcc gccaaacgca tgaaccgcaa catccaacgc gcgctgacag gccagagtgt
     7441 cgataatatt tgatacttaa acggtttcag acggcataga aataatccga tgccgtctga
     7501 aggctcgaaa acacttgaaa aacatcgata tggaaaagtc aggcattgtc tatctgatga
     7561 aaaccgtcat caagggcgtg tataaaatcg gcatttcgga tgtaagcaat tttgaaggca
     7621 gaatgcgcca tttggaaaac aacggttatg cgaacgttgc cggattggaa cgcatcctcg
     7681 ccgtcaaaac cgacaattac aaagaaaaag aaaacctgct ccatgaaatt ttcagcaaaa
     7741 gcaggatagg cgataccgaa ttgttcgccg tggacgaaaa ccttgtgaaa cgtttgtttt
     7801 tatcgcttcg cggcgaaatc gtgttcccga aaaacgaaac ggcggaatcg gaatttgaaa
     7861 aaagcgtcca cgaacgcagg caggaaggga atgccgggtc aggccgcaaa caactgcttg
     7921 atttggtacg gcgcggacac cgggaatacc cttacgcgct gccccggctt ttggcgggcg
     7981 cggcatccta caagccgaaa aaatcgaaaa tccgcctttt taaagaagca tatttcggca
     8041 aaagcggcac gaggctgacc gacgaaattg cagacggcat ccatatttac acctgttttt
     8101 cgcgggcgga tttggaaaaa gcctattccg aatatttgga acttttcaaa tccgaatcgg
     8161 atgccgaagg cagaaagccg cagtaaggtg caaacagata ccgtacacgt tgaggagcag
     8221 atatgatggg cgattccgtc atttattatg tagaacaggc agacgaaccg gtaaaccgtg
     8281 ccgacgaacg cgcccgtaaa acattcaaat atttttggcg cgagcttttt tgggaacgcc
     8341 gccgcattat ttccgccttg gattttgcca tggtcaaagt cccttttttc caagacggcg
     8401 aagacggcga aatttgcgaa catatgtgga tcgacgatat ttattttgac ggtctttata
     8461 tttacggtgt gctgaacaat gaacccggcg aactgaccaa tgtcgaacaa ggcgaaagcg
     8521 tttgcgttcc ggttgacgac atcagcgact ggatgttcgt gtgcaacggc atcccctacg
     8581 gcggctttac catacaggca atgcgcgggc agatgacgga agaggagcgc accgaacacg
     8641 atgccgcatg gggaatcgat ttcggcgatc ccgggcagat attgctggtg tatgaagaaa
     8701 aagaacatcc cgaaaatctg gaagagcatc cgatgtgccg gaactgtatt gacgattttc
     8761 ggcaacagtt gtcccaaaac tcggattatc tgcgggaaca ggacgaagac ggctatacgc
     8821 cgcttcatca tgaagccatc gcaggaaatg cacttatggt tcaagccatg cttgaatacg
     8881 gcgcaaatcc tgcctcaacg acatcggaag gctataccgc cctcgatttt gcctgcctga
     8941 cgggctggca aaatgttgcc gacctgctcg aaccgcgaca ttaggcagac agttttccga
     9001 aaacgaacac aaacactttt tacagaaaga caataaaaat gcccaaaatc accgtacttc
     9061 cacacacgac attatgcccc gagggtgcag tcatcgataa cgcacccgaa ggtaaaaccg
     9121 tccttgacgt gctgctcgac catgatatcg aagtcgatca cgcctgcgaa aaatcctgcg
     9181 cctgcacaac ctgccacgtg attatccgca aaggtttcga cagcctagaa gagccgaccg
     9241 aattggaaga agacctgctc gatcaggctt ggggtttgga agccgattcg cgcctgagtt
     9301 gtcaggcggt tgtcgccggc gaggatttga ttgtggaaat ccccaaatac accatcaacc
     9361 acgcgcgcga agaacactga aaacaggccg tctgaagccg gcacgcttca gacggcattg
     9421 ttgcgcggat aaggcgcaat cgcccgaaaa caggcgttcg tacaggcgga actttcgatt
     9481 ctatagtgaa ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta cagatagtac
     9541 ggcaaggcga ggtaacgctg taccggttta aatttaattc actatatatt gatttttatc
     9601 ggttttctga cggaataatc cagtgcggca tccgaggcgg attactcgga cgcgatgcac
     9661 cggtatttat cggttttgca gccggaaaaa ccgccggcgg gttatagtgg attaaattta
     9721 aaccagtaca gcgttgcctc gccttgccgt actatttgta ctgtctgcgg ctcgccgcct
     9781 tgtcctgatt tttgttaatc cactatactt ttagggcgac ggtcgggcag tatgccggat
     9841 agcgttccac tctcgcttct atattgattt tgattggttt tctgacggaa tgacccgatg
     9901 cggcatccgg gacggcttgt gttttttcct gcccgcctgc cggattttcc catccttgcg
     9961 tgaaaccgaa agagacggcg gcgcggcgga caagctcgag atagcgtcct tcaagctccg
    10021 gacaggcggc ggacacgctt tctaccgtaa ccgtgaaacc gccgcccgac ccggcaaggc
    10081 gttccgcaat ttgcgtgtag atgagccaac gttgggcgcg caacattgcc gaatcgcaac
    10141 cggcagccgc tttatccaag gcaatcaggt cgcgccggtt ttggtggtga aggcaaaccg
    10201 ttactctgga gaggatatgt tgcccgtcca atatttgata cagtttgcgt atcagcagaa
    10261 tcaggcggtc aaactcctcc atctccgaca gggaatcagg atggtcggaa gcggtataaa
    10321 ccagtttgga caattttgcg gcaaacatat tgttcatcaa tcttccttgt cggttgaaac
    10381 cccgctcttc ggggcggtag aatcagattt gtttgggagg gacaactcct cccagatcag
    10441 gacgacacat aggctggtgc ttgatgtgtt gtccggcgag ttgaaacatt cagcaatcct
    10501 caaggggcgg cagttttgcc gaaacatatt ctacacggct tcaatgccgg acgataaaag
    10561 gaaattcata tgaaatggac cgacacccag cgcatcgccg aagaactcta tgacctgcac
    10621 ggcgaaacca tcgatcccag aaccgtgcgc tttacccaac tgcgcgacct gattatggca
    10681 ttgcccgaat ttgacgacga ccccgcccgt tgcggcgaac gcatcctcga agccgtgcag
    10741 caggcatgga tagacgaggc ggaataagtt tcgggaatgc cgtctgaaat gcggcggtac
    10801 gcggttcgtg cttctgtttg cagcgggaat ggttttacca gtctcctttt ttcagcctgt
    10861 ccagttggcg gcggtcgcgc ttggtcggtc tgccgtcggg ataggcggaa gtgatgcggc
    10921 tgaattggtc gagctgtttg cgctcttccc tcaatgttgc cgttttcgcg tcctcttcat
    10981 acagaagccg cgcctcggat gccgggcggc gttggtggtt caaaccttta accttgattt
    11041 tatagggaag ggaattgagc gtcaggtcga taatatcgcc gatgtctatg gttttactgt
    11101 ttttgacctt cgagccgttt acttgaaccc tacccagttc gatgtgcttt tgcgcaaggg
    11161 aacgggtctt gaaaaaacgt gccgcccaaa gccatttgtc cagccgcatg gcggaagaat
    11221 cgtgcttgtc tttcatacga ttttgtttga aataattgaa tttgtttcga gtttagcata
    11281 agatacgccg ccttataact agtatatatg cactaatcca ctgttttcca tgctgtccga
    11341 acacaaaaag agggtatgga aaagccgttt tggacaataa attaactgcg gaatatgcac
    11401 aaatagcgta tgatagcggc agaatctgtt gatgagagct tcattctatg aaacctgttt
    11461 ttttggattt tgaacaaccc atagccgaac tgaccaacaa aatcgatgag ctgcgtttcg
    11521 tccaagacga gtctgccgtc gatatttcgg acgaaataca ccgtttgcag aaaaaaagca
    11581 acgacctgac caaatcgatt ttcagcaaac tcacacccgc ccaaatttca caggtttccc
    11641 ggcatccgca gcgtccctat actttggatt acattgaggc actgtttacc gattttgaag
    11701 aactgcacgg cgaccgccac tttgccgacg attatgcgat tgtcggcgga ttggcgcgtt
    11761 tcaacggaca aagcgtgatg gtcgtcgggc atcagaaagg gcgcgacacc aaagaaaaaa
    11821 tccgccgcaa cttcggtatg ccccgtcctg aaggctaccg caaagccctg cgcctgatga
    11881 agacggcaga aaaattcggc ttgcccgtaa tgacctttat cgatacgccg ggcgcgtatc
    11941 ccggcatcgg cgcggaagaa cgcgggcagt cggaagccat cggcaaaaac ctgtacgaac
    12001 tgacgcgcct gcgcgttcct gttttgtgta ccgtcatcgg cgaaggcggt tcaggcggtg
    12061 cgttggcggt cgccgtaggc gattacgtca atatgctgca atactcgacc tattctgtta
    12121 tctcccccga aggctgcgcg tctattttgt ggaaaaccgc cgaaaaggcg gcggatgcgg
    12181 ctcaggcttt gggcattact gctgaccgcc tgcaaaagct ggacttggtc gataccgtca
    12241 tcaaagaacc attgggcggc gcgcatcggg atttcgggca aaccatgaaa aacgtaaaag
    12301 ccgttttgga aaaacaactg cacgaagcgc aaagcatccc gcttgccgat ttgctttcgc
    12361 gccgtttcga ccgcattatg gcttacggca aattttcgga acaataattc aggtagaaca
    12421 agcagcaagc agtttgtctg aaactgcttg ctttttcttt atcgggacgg aaccgtgctg
    12481 actttagatg cgtttgagca atgcttgaag gattgttttc ctcaaggtct gaatggaaaa
    12541 aaaacagcgg tggcattaag cggcggcttg gattccgtcg ttttgctgca tctgcttgtc
    12601 cgcgccggaa aaaagggcgg ttttattccg gatgcattgc atatccatca cggcttgagt
    12661 ccccgtgccg acgattgggc agatttctgc caaaactatt gcgatatgct cggggtgggg
    12721 ctggaaacgg ttaaggtctg cgtggaaaaa aacggtttgg gcatcgaggc ggcggcaagg
    12781 caaaagcgtt atgccgcgtt tgccgaaaaa ggctttgacg ttttggcgtt ggcgcaccac
    12841 agggacgatc aaatcgaaac ctttatgctg gcggtcgcgc gcggcggcgg tttgcgcgct
    12901 ttggcggcta tgcccgccgt ccgccctttt ggggaaaaag gcatcatctg gcggcccttg
    12961 ctgccttttt cacgccaaga tatatgggat tatgcccaaa aacacggttt gccgaatatc
    13021 gaggatgaaa gcaataccga tacggcttat ttgcgaaacc gcttccggca ccgtattttg
    13081 cccgaacttt cggcgcagat tccccatttc gggcggcatg tgctgaacaa tgtccgcgct
    13141 ttgcaggaag atttggcttt gttggacgag gtcgtcgttc aggactgccg ttgggtttgc
    13201 ggggccggtt atttcgatac ggcgcggtgg ctgacgtttt ccccgcgccg gaaaacccat
    13261 attttgcggc attttctgaa ggaaaacggc attcccgtgc cgaatcagaa tgcccttgcc
    13321 gacattgccc gggttttgac ggaggcaaaa accggacgtt ggaacttgca aggctttgaa
    13381 ttgcatcatt atgcaggcag gctgtttgtg ttccgactgg aaaaaacgga taaactgcgg
    13441 tttttgaaag acaggcagat aagcggaaat ttaagggaaa tattgacggg gcagggattt
    13501 gtgttgaagc ggcatccgtt tgggcttcct gagcatcttt tggagcagga cggaattttg
    13561 aggacggtag cggcatcgga tacgttggcc atgggcggca tccataagga tgtgaaaaaa
    13621 atccttcagg ggaaacgggt tttgcctgtc ctgcgcccaa tttggccgct tgttgccgac
    13681 agcggaaacc gtccattggc gttggcaaac tgttgtgcgg atttccaata ctcggtttca
    13741 gacggcattt tgcccgtcca tcctgacttt cccattttat tttgataata tcgcaaacag
    13801 atttcggcgg cgttcagtcg ggtattgtcc ggttgcatat ttctaaaagg cttgtgaagt
    13861 gaaacacatc agttcgacca ataatgaaca catcagacac ctgcaccgcc tgttgtcgca
    13921 aggaaagttc agacggcaat acgcccaaac cgttttggag ggcgtgcacc tgcttcaggt
    13981 tttcctgcaa tccggcggga tgccggtcgg ggtatatatt cccgaagcga aaatgccgtc
    14041 tgaagaagtc cgtaaattga cggcggtttt gccggaagac gggttttttt ccgtttcaga
    14101 cggcatattg aagaaaatca gcagcctgac ttgtgcggat gatgtgcttg cgctgattga
    14161 tattccagat gcgggtgctt tgccggccgg cggcgattgc gtggttttgg acggcgtgca
    14221 agacccgggc aatgtcggca cggtgttgcg aagcgcggcg gcggcgggaa tcggcgcggt
    14281 cattttgggc aaaggttgtg cggacgcgtg gtcgcccaaa gtgctgcgag ccggaatggg
    14341 cgcgcatttc ttgtcggaga tttatccgca ggcggatttg gaaatatggt tggtgcgcta
    14401 taaaggccgt gtgtttgcca ccgccttgcg cgaggaaaag caggcggttt tgtacggcga
    14461 agatttgtgc gaaccgacag cctgggtgtt tggcaacgaa ggcgcggggg tcggtaaagc
    14521 agttttagat agggcggaca agtgtgtcag gataccgatg cacgatgcaa ccgagtcttt
    14581 aaatgtcgcg atggcggcga caatctgcct gtttgaacaa atgcgccaac gggcggcgta
    14641 ttgaggaaga gaaatgccgt ctgaaaaaat ctattacggc gtattgattt tcttatgtat
    14701 cgcttctatg ctgctgtcgc cgttttttta tgcgggtgct ttgaagccca agaaggcggc
    14761 attgcggaag gacgggcagt ggaaactcat ctgattgtcc aatgccgtgg cggcggcggt
    14821 tttggcttgg gtgtggtgga aatggttttg acagatattg ctcaaaatcg tgctaatgga
    14881 atccgaacaa ataaagaatt tggtaaaaaa tttgttaaat caacgaatta aagttttgtg
    14941 gaaaacaaaa cagctctaag caaatagggc gtttgtcggt aaatacggaa gagttgcggc
    15001 attatcgggc atctttaaca agtagtgccg tcttgacagg caatcggttt ttatgggcag
    15061 cttgcaaaat cgcggatata aaattgcgaa tcggttaaag tgtggggacg ctatgaaaaa
    15121 ttgcgaattt tttatgaccc gacaagggca atctatgata gcggtgcaga ttacttaact
    15181 agggaaaaac atagattagt cgtaattgca aatagtgctt gggggctatt gcttaattta
    15241 tcttgttatt atgacgaggt tttggaaaag cggaaaatac cgttcggcaa acaggaaatt
    15301 gatgacgata tggacaaagt gtccgccctt aagcggaagt ttaaagatat ttctgaaatc
    15361 aaagtagggg atggttggga atacccgttc aattatgagc agggaatgaa agaattagat
    15421 gaagtgctat tgaaatacat tccctttttt gaagaagaac gataaaggag gttgatatgc
    15481 gcgtatctaa aataattgga agtatgttgc ttgttacagc ggttcagacc gtattttcgg
    15541 caaatgttta cgcgtgccgc cataatggta aaaccagtta cagccaaact ccgggaaaac
    15601 attgtaccaa cgcgggtttg gggcgggacc gggtgtacag ttcggttaga ccggcagtaa
    15661 aagacagggc ggaagacgca ggggtcggcg attattcgga cacggtgagg gacgaacacg
    15721 tccaaaatcc gaaaggaaat gcacagaaag acggttcggc tgccggcatc aagccgcact
    15781 gattgaagcc gaatcagccc ttgcgctgtc ggacggcaaa atttgaacga ttggggaact
    15841 ttgatgaaac acatccatat tatcggtatc ggcggcacgt ttatgggcgg gcttgccgcc
    15901 attgccaaag aagcggggtt tgaagtcagc ggttgcgacg cgaagatgta tccgccgatg
    15961 agcacccagc tcgaagcctt gggtatagac gtgtatgaag gcttcgatgc cgctcagttg
    16021 gacgaattta aagccgacgt ttacgttatc ggcaatgtcg ccaagcgcgg gatggatgtg
    16081 gttgaagcga ttttgaacct cggcctgcct tatatttccg gcccgcaatg gctgtcggaa
    16141 aacgtgctgc accatcattg ggtactcggt gtggcgggga cgcacggcaa aacgaccacc
    16201 gcctccatgc tcgcatgggt cttggaatat gccggcctcg cgccgggctt ccttattggc
    16261 ggcgtaccgg aaaatttcgg cgtttccgcc cgcctgccgc aaacgccgcg ccaagacccg
    16321 aacagccaat cgccgttttt cgtcatcgaa gccgacgaat acgacaccgc ctttttcgac
    16381 aaacgttcta aattcgtgca ttaccgtccg cgtaccgccg tgttgaacaa tctggaattc
    16441 gaccacgccg acatctttgc cgacttgggc gcgatacaga cccagttcca ctacctcgtg
    16501 cgtaccgtgc cgtctgaagg cttaatcgtc tgcaacggac ggcagcaaag cctgcaagat
    16561 actttggaca aaggctgctg gacgccggtg gaaaaattcg gcacggaaca cggctggcag
    16621 gccggcgaag ccaatgccga cggctcgttc gacgtgttgc tcgacggcaa aaccgccgga
    16681 cgcgtcaaat gggatttgat gggcaggcac aaccgcatga acgcgctcgc cgtcattgcc
    16741 gccgcgcgtc atgtcggtgt cgatattcag accgcctgcg aagccttggg cgcgtttaaa
    16801 aacgtcaaac gccggatgga aatcaaaggc acggcaaacg gcatcaccgt ttacgacgac
    16861 ttcgcccacc acccgaccgc catcgaaacc acgattcaag gtttgcgcca acgcgtcggc
    16921 ggcgcgcgca tcctcgccgt cctcgaaccg cgttccaaca cgatgaagct gggcacgatg
    16981 aagtccgccc tgcctgtaag cctcaaagaa gccgaccaag tgttctgcta cgccggcggc
    17041 gtggactggg acgtcgccga agccctcgcg cctttgggcg gcaggctgaa cgtcggcaaa
    17101 gacttcgatg ccttcgttgc cgaaatcgtg aaaaacgccg aagtaggcga ccatattttg
    17161 gtgatgagca acggcggttt cggcggaata cacggaaagc tgctggaagc tttgagatag
    17221 cccgggcgat gccgtctgaa agcccttcag acggcatcgc ccggctgcgc ggcacaaagg
    17281 cggaaaaacc gtttgccccg tattttcaaa cgcgttacac ttgccgccgc tgttttcagc
    17341 catttgatta cccgcaaccg ccgtcattgc gccggcggtt tgcctgtcag cgtcattgcg
    17401 ccgctgtaaa tacgaaagaa cacattatga ccgtatcccc cgtcgccttg cgccgtaaga
    17461 ccgagtgcaa gcctcatccc accgcgcgct attggaaaaa atgcgatgtc gaagccctgt
    17521 tcggacttcc cttcctcgac ctcatttacc aagccgccga aatccaccgc caaaatttca
    17581 acccgcgcga aatccagctt tccacgctgt tgtccatcaa aaccggcggt tgtcccgaag
    17641 actgcgccta ttgtccgcaa tcggcgcacc acaataccaa tctgggcaaa gagcagatga
    17701 tggatgtgga tgaaatcgtc gaaaaagcca aaatcgccaa atcgcgcggc gcaagccggt
    17761 tttgtatggg cgcggcgtgg cgcggcccta aacccaaaga cgtggagacg gtttccgcaa
    17821 tcatcaaagc cgtcaagggc ttgggtatgg aaacctgcgg cacgttcggt atgctcgaag
    17881 aaggtatggc ggaagacttg aaagaggcgg gcttggatta ttacaaccac aacctcgaca
    17941 ccgaccccga ccgctacaac gacatcatcc acacccgcca acacgaagac cgaatggaca
    18001 ccttgggcaa agtccgcaac gccggtttga aagtctgctg cggcggcatc gtcgggatga
    18061 acgaaacccg cgccgaacgt gccgggctga ttgccagtct cgccaatctc gacccgcagc
    18121 ccgaaagcgt gccgattaac cggttggtca aagtggaagg cacgccgctt gccgatgccg
    18181 aagatttgga ctggacggaa tttgtccgca ccatcgccgt ggcgcggatt acgatgccgc
    18241 aaagttatgt ccggctgtcg gcagggcgca gcaatatgcc tgaagcaatg caggcgatgt
    18301 gctttatggc gggcgcgaac tcgatttttt acggcgacaa gctgctgacc acgggcaatc
    18361 ctgatgagga cggcgacaga atcctgatgg aaaagctcaa cctgtatccc ttgcagtttg
    18421 aaccggaagg cgaggtcgcc gaagtggaaa aagcctctgg gattaaagtg gattattgac
    18481 gattgaaaaa tgccgtctga aacccggaaa aaggctttca gacggcattt gtccggacgg
    18541 catttccaat atctttttac cggcgcgtga tgctgccgtc gggcgagaca tccagcccgt
    18601 tccccttggg gaaatcgctg cggttcagat aaataatccg cccgataacg gttttctcgg
    18661 ggtcgatttt ggggatttta tcgccgactt gagtgatggg gataatgttg ccggaaacga
    18721 accgcccctg tttgtcggtg ataattttaa aaatgggggc gatgccgctg atgccggagg
    18781 tgttgattgc gccgtaggtg gcaaagttgc cgccgctgta ggagatgaag cggtcgcggt
    18841 aaagttcgac ggcgcgagta acgtgcggcc cctgcccgaa tacgacatcc gcgccggaat
    18901 cgacggcaag ccgcgcaaac tcaacgacgt tgcccctgtt ttccccatag aagatttcgg
    18961 tatcgaacgg caggtgttcc gcctgtttcc cttccgcgcc gccgtggaac atcacaatga
    19021 cgatgtcggc tttctgtttg gttttggtaa tccgttttct aactttggca taatcgttca
    19081 gtttgacggc ggcaaggttg ggggcgaagg agacgaagcc ggatcttacg ccgtttttct
    19141 tcaggatggc ggtttcaaac ctgttttcga tgcccgaata tttgatgttc aattcgtcaa
    19201 ggttcgccct ttatgccgtt tccgcaccgc aaaccgccgg ggtcaagccc tcggcgcatc
    19261 ctgccggaac ggaatccccg tgctgccgat tgacggttca aaccccgcgc ccgtttcaaa
    19321 taccggcgat gtggacggac aggatgcgcc tgacgaaaag acagccgata ccgtttccat
    19381 tatcggcgtg ggcgacatta tgctcggcag caattatccg gtcgattacc tgcccgatac
    19441 caatattctg aaaaacgtcg aatctgcctt gcaagacgcg gacattaccg tcggcaacct
    19501 cgaaggcacg ctgtttgacg aaggcggtac gccgaaaaaa tgtgcaaacc cccaaaatat
    19561 gctatgcatt ccgaacgccc tccgcatacg ggcaatacct tgccgacgcg ggattcgact
    19621 acctcagctt cgccaacaac cacagcaacg acttcggcgc gcaaggcatc acggcaacgg
    19681 cggcgagcgg cagctctttt acatactcga tcgcgctaaa gccgctgccg ataacgaggc
    19741 caaaattgcg gaaaataccg ccatcgccca gataaatttg tccatcatca gacctttact
    19801 gttcagacga gacagcattt gccgcacgtt ttggggctta tctttcgatt tgcgctacgt
    19861 cgcgcaccgc gcctttgtcg gcggaagtcg ccatcgcgcc gtaagctctt aatgcggcgg
    19921 agacgtagcg gtcgcggttt ttaggcttcc atgctttgct gccgcgcgct tccatttcgg
    19981 cacggcgtgc ggcaagctct tcatcggaaa tggcaaggtg gatgctgcgg ttggggatgt
    20041 cgatttcgac ggtatcgcct tcgtgtacca aaccgatcgc gccaccttcc gccgcttcgg
    20101 gcgaggcgtg tccgatggac aaacctgatg tgccgccgga gaagcgtccg tcggttaaga
    20161 gagcgcaggc tttgccgagg cctttagatt tcaggtagga agtcggatac agcatttcct
    20221 gcatgcccgg gccgcctttc gggccttcgt agcggatgat gacgatgtcg ccagcgacga
    20281 tttggttgcc caaaatgcct tctactgcgt cttcttggct ttcaaacacg cgggcgcggc
    20341 cggtgaattt gaggatgctc tcgtccacgc ctgcggtttt taccacgcag ccgcgctcgg
    20401 cgatgttgcc gaacaagacc gccaaaccgc cgtcttgcga gtaggcgtgt gccacgtcgc
    20461 ggatacagcc tttttcgcgg tcgaggtcga gggttttcca catacggttt tgcgagaacg
    20521 cttgggtggt gcgtacgccg cccggcgcgg ctttgaagcg ttcgatggca cgggtgtttt
    20581 cgggattggt cacgtcccat tgttcaatcg cgtctttcag cgtcggcgcg tggatggtgt
    20641 gcacgtcggt gtgcagtttg cccgctttgt ccagttcttt caggatggcg aagataccgc
    20701 cggcgcgatg cacgtcttcc atatagtagt cgtggttgtt gggtgcggtt ttgcagatgc
    20761 agggcacgac gcggcttaag cggtcgatgt ctgccatttt gaaatcgaca ccggcttcgt
    20821 tggcaacggc caacaggtgc aaaatggtat tggtgctgcc gcccatcgca atatccatcg
    20881 tcatagcgtt ttcaaacgct tttttggtgg caatgctgcg cggtaacacg gtttcatcgt
    20941 tttgctcgta atagcgtttg gtgatttcga caatcatacg gccggcttcg aggaacaatt
    21001 ctttgcggcc ggcgtgggtc gccaaatacg aaccgttgcc gggcagggaa aggccgagtg
    21061 cttcggtcag gcagttcatc gagtttgccg taaacatacc cgaacacgag ccgcaggtcg
    21121 ggcaggcgtt ttgttcgact tcctcgactt gccggttgct gacattgtcg tccgccgatt
    21181 caatcatcgc gtcaatcaag tccaaacggc gttcgggctg gatgtttgcc acgccgataa
    21241 ccttgcccgc ttccatcggg ccgccggaga cgaagatggt ggggatgttc aggcgcatcg
    21301 cggcaatcag catgcccggg gtgattttgt cgcagttgga aatgcacacc agcgcgtcgg
    21361 cgcagtgggc gttgaccata tattcgatag agtcggcaat caaatcgcgg ctgggcaggg
    21421 agtacagcat gccgctgtgt cccatagcga tgccgtcgtc gatggcgatg gtgttgaatt
    21481 ctttggcgat tgcgccggct ttttcgattt cgcgggcaac cagctggccc atattgtgca
    21541 ggtggacatg gccgggcacg aattgggtga aggagttggc aacggcgatg atgggcttgc
    21601 cgaagtcggt ttccatcacg ccggtggcgc gccacaatgc acgtgcgccc gccatattgc
    21661 ggccgtgggt ggaggttttg gagcggtatt caggcatagt gtgtttcctt gtgcctatac
    21721 cgtctgaaag acagggctgt ttcagacggt atcgggtacg gttttttaga gtgggaaaag
    21781 agggtatttt ataccaagta tcggaatttt gcgggattga aacggcgtgc ggcaaaaaag
    21841 aaaatccccg caggaatgcg gggacgggtt caggcgcggg caatcgcgac ggctttggat
    21901 gcgtcccaaa aatcaacggg tgcatttaat acgggtttga cgatgcccgt ccgtatggcg
    21961 aaatcgccga aaccttcgcc gatattgcgt tctgccgccc atttgccgat caggtcgtcc
    22021 aattcggcaa ggatttcggg cagggtgatg ttttctttgt aaagacgggg gatgcgtacg
    22081 ccttcacggt cgccgccgat atggaggttg tagcgtccga cggctttgcc gaccagtccg
    22141 atttccgcca acatcgcccg tccgcagccg ttcgggcagc cggtaatgcg ggtaacgatg
    22201 tagtcgtccg acgtgccgtg tttcgccata atcttatcca gctcgccgat gaagtccggc
    22261 agcacgcgtt cggcttccgc cattgccagc gggcaggtcg gaaaggaaac gcaggacatc
    22321 gcattttcac gcagcttgct gacatcgttg cggattaatc cgtatgttcg ggcaaattct
    22381 tcgatttttg ctttgtctgc ttcggcgaca tttgccacga tgaggttttg gttggcggtg
    22441 atgcggaaat cgcctttgtg gattttggcg atttccaaca cgccggtcag aagctgtttc
    22501 ccgccttcgt caaccaaacg cccgctttcg atgaaaaggg ttaaatgcca gttgccgtct
    22561 atgcctttca cccagccgat gcggtcgccg cgcccggtaa atttgaacgg gcgtacgggt
    22621 tcgaacggca tacccatacg gcgttcaact tccgcgcgga agttgtccaa gcccatattt
    22681 tgaatggtgt agcgggtgcg ggcgtttttg cggtcgctgc ggttgccgaa gtcgcgctgc
    22741 gtggttacca ccgcttcggc ggccttcagc gcgtgttccg gaggcacgaa acccagttcc
    22801 agtgaaatgt tcggataggt tttggtgttg ccgtgttcca tcgaaagccc gccgcctgcc
    22861 aaaacattga agccggcaag ctgtccgtta ccgtctgaaa cggcgacgaa atccaaatcg
    22921 ttgccgtagc agtccacatc gttcaagggc gggatgacga ctgcggtttt gaattttcgc
    22981 ggcagatagg ttttgcccaa aatcggctcg tcttcttgaa ggaagtcgtc ggaactttga
    23041 acttttttgc cgtccaccca cacatccaga taaccgcgcg tgcgcggcag caggtgttcg
    23101 gaaatctttt tcgcgtattc gtaagcctgc cggtgcagtt cggactcgat cgggttggac
    23161 gtgcaaagca cgttgcggtt catatccgcc gccgtggcga tggaatccaa acccagtttg
    23221 tgcaagaggc ggtgcatcgt ctgcaacttg gctttcggca cgccgtgaaa ttggaaggtt
    23281 tgccggttgg tcagccggat ggagcggtaa tgactgtttt cccgggcaaa tttgtccagt
    23341 tctatccatt gggacggttt gatgatcccg cccggcagcc ggcagcgcaa aagcataaat
    23401 ttcaagggct cgagttttgc ctcggcgcgt tcggcgcgga tgtcgcggtc gtcctgctca
    23461 tacataccgt ggaagcggat gagttggaag ttgtcgcctt tgaagccgcc cgtgagcggg
    23521 tctttcaaat cgtccaaaat cgtgccgcgt aaaaaattgc tttctgtttt cagacgttcg
    23581 ttgtcggata gcggtttttc ttgccacgcc aaaccttttg tcttggtctg tacggtcatt
    23641 ttgtgttcct cccgattata tttaatcaat aaacatcacg ctgatagcgt ttttcttcgc
    23701 gcagcatatc caaatattct tctgcgccct cttcgtccaa atgtcctgcc ccgataatca
    23761 catccagcaa ggcggcttcc acgtcttttg ccatttttgc cgcatcgccg cacacataga
    23821 tatgcgcgcc ttcctgcagc cattgccaaa gtccttccgc ctgttcgcgg attttgtcct
    23881 gcacatagat tttttcttcc tgatcgcggg accaggcgaa atcgtacctg tgcaggaagc
    23941 cgtctttggc aaactgctgc cattcggttt gatagagaaa atcacgggca aaatgcggat
    24001 tgccgaaaat cagccagttt ttgccttccg cattttctgc ggcacgttgt tggacgaaag
    24061 cgcggaacgg tgcgacgccg gtgcccgagc cgatcatcac aatcggcttg cggctgtctt
    24121 cgggcagcct gaagccgtcg ttgcgttcca caaacacgcg caccgtgccg tcctcttcca
    24181 gccggtcggc aaggaaaccc gatgcgccgc ccgttctggc gcggccttcg tgttcaaaac
    24241 gaaccacgcc gacagttaaa tgcacttcat cgcccacttc cgcctgtgct gaagaaatcg
    24301 aatacaaacg gggtgcaagc ggacgcagta aacggatgaa ttgttctgcc gtcaggcttg
    24361 ccgggaagcg gtgcagcaca tcgacaatag gcgtgttttg cacgaaatcc tgcaaaacgg
    24421 cgttatcggc aatgatttta tcgagttctt cataatgggc gaacgcggca tagcctttga
    24481 cgaaagccgg agtgttttgc gtgagttcga aatgagatga aagtgcgcgc gcaaccggca
    24541 tcatctttcc gcccgcctgt atttccgttg ccggatcgat gccgagcagg tctaggattt
    24601 ccctgaccag tgccggatcg ttgtcaaacc aaacgccgag cgcgtcgccc gggaggtagt
    24661 gcaaatccga accgctcaaa tcgatttcga tgtggcgcac gtctttatcg gattggcggg
    24721 cggtgatttt ctgattggcc agcagggcgg cgggaaaggg ggctgccttg cagtacctgc
    24781 catccggtgc cgtctgaagg ccggcggggg gcgttgtctg cggcgcgggc gttgcccggt
    24841 tttttgcggc ttcttctttt aagagtgcgg cgatattatc tgtccaggcg tttgcggagg
    24901 cggtaaagtc caaatccgca tcaacgcgtt cgagcagccg ttttgcgccc aattcttcaa
    24961 aacgccggtc gaaatcttta cctgcctgac agaaattcgg ataggaactg tcgcccaaac
    25021 ccagtacggc aaattggagt ttgtccaatt tcggggcttt tttgccgttc agcagtttgt
    25081 gcagcacgac ggcttcttcc ggcggttcgc cttcgccttg ggtggaggta accagcagca
    25141 ggcggcgttc gccggcgatg tttttcgcct tatagtcttt cagttcggcg cgactgactt
    25201 ggatgccggc ggcttccagg ctgtccgccg ctttgtcggc aacggatttc gcattgccgg
    25261 tttgcgaggc ggaaaggacg gttacggaaa aaggttctgc cgccggcaat gccgtctgaa
    25321 gcgcgggcag tcctgcagat gccccgtttc ctgcttttgc ccaagcgtag ccggacagcc
    25381 acgcccattg tgccgcgtcc agccccgaca ggagctgcgt gatttcgggc ggcagaggcg
    25441 gtaatggcgg atttgtgttc tgcatatcgt gttcactcat aaaatcatac ctgccgcaac
    25501 agtgccgtat gtcgcttcgt ctatcaggat aaacgaaccg gcggcggtgt tttccgcata
    25561 aggcgttgcc gtaacgggtt tttgaaggtt gatgcggact ttggcgatgt cgttcatctt
    25621 caaggattcc gcgccggcct cttgttccag cgtgcggaca tccaaaacgc tttcaatttc
    25681 cccgactttt gccggcacgg tttgcgtgcc gtgcttgagc aggtatttgc gcgcggtgtt
    25741 gagcggacgt tcgtcaaacc agcaaagcgt ggcttccaga tgtttttgcg gggcgagcgg
    25801 ggaattttta tcgacaaaaa ggtcgccgcg cgaaacatcg atgtcgcggt ccagccggag
    25861 ggttgccgcc tcgccggcaa aagcctgcgc cacttcccct ttcggcgtga tgatttcgga
    25921 cacttcggcg gtcagcccgt tcggttcgat gcggacggtt tgcccgacgg cgaccgaacc
    25981 gcgttcgatg cgcccctgat agcctcggaa atcatcggcc ttgtcggcat cttggcggac
    26041 gaccagttgc acggggaaat aaaaatcgtc ggcggtgcgg ctgacttcgt ccgcccccgg
    26101 cagggtttcc aaaatggaca ataagggttc gcctttatac caaggcatat tgccgccggg
    26161 gtaaacaatg ttgtcgccca agagtgcgga catcggtacg aaatgcgcgt ctttcaaacc
    26221 gagctgttcg gcaagtcggc ggtatgcctc cacaatggcg ttgaatttgt cttcgctgta
    26281 atccagcagg tccattttgt tgaccgccac cacaatatgc gggcagttga gttggcggag
    26341 gatggcggaa tggcgtttgg tctgcggcag aagctgcaag ggctgcgcgc cgaaatccag
    26401 ttgggatgcg tcaaccagca cgactgccgc cgaagcggtg cttgcgcccg taaccatatt
    26461 gcgcgtgtat tgttcgtgcc ccggcgtgtc ggcgatgatg aatttccgtt tcgccgtgga
    26521 aaaatagcgg tatgccacat cgatcgtaat gccctgttcg cgttcggctt ccagtccgtc
    26581 ggtcaggatg gagaagtcta tggcttcttt caaacctttg cttttgccgg attccaaggt
    26641 tttgatttgg tcggacagca gggctttgct gtcgtagagc agtcgtccga tcagggtgct
    26701 tttgccgtca tcgacgctgc cggcggtaat gaagcggagc ggggtttggt gttgtgccgt
    26761 catattttct tcctcatatc tgcttaaagg gtttttgaaa tttagaaata gccttctttt
    26821 ttgcgttttt ccattgccgc ctcgcttgcc tgatcgtcca gccgggtcgc gctgcgttcg
    26881 gaaatgtcgg caaccgctgt ttctctgata atctccgtcg gcgtggacgc ggtgctttct
    26941 accgggcagg tgcagctgat gtcgccgacg gtgcggaagc ggacatcaag gatttcggag
    27001 gtttcagacg gcattttcgg ggtgagcggc gttacaggga ccagcagccc cctgcgtctg
    27061 accacttcgc gcctgtggct gtaataaatc ggcggcagct cgaggttttc gcgggcgatg
    27121 tattgccaga tgtcgagttc cgtccagttg gaaatcggga agacgcgcat attttcgcct
    27181 ttgtgcagcc tggtgttgta cagcgaccac agctcggggc gttgcgcctt cggatcccat
    27241 tgtccgaact cgtcgcggaa cgagaaaatc cgttctttgg cgcgggcttt ttcttcgtcg
    27301 cgccgcgcgc cgcccataag cgcgtcgaag ccgtttgcct cgatggtttc caacaaggta
    27361 accgcctgtg ccgcattgcg cgaatcggtt tctttgcgta agaccaccgt gcctttggca
    27421 atggagtctt ccacgcgccc cactatcagg cgggcattga gttttgccgc ctgcgcgtcg
    27481 cggaaggcaa tcacttcggg gtagttgtgt cccgtgtcga tatgcaccag cgggaagggc
    27541 agtttcaccg gccggctgcc cagccggaag gctttgcagg cgagggcgag caggaccacg
    27601 gaatctttgc cgccggaaaa gagcagggcg gggttttcgc attctgccgc cacttcgcgg
    27661 atgatgtgga tggattcgga ttccaaccag tcgagttggg cgttgttcgg ttcggttttc
    27721 gtcataccat attccttatt tcttctgtct gatatttatg aattatttgt gcagcccgca
    27781 ttctttgctg tttctgcctt cccaccacca ccgcccggcg cggatgtctt cgcccgcctt
    27841 gacggggcgg gtgcaggggt cgcagcctat gctgggaaat ccttgccggt acaaatcgtt
    27901 gtaaggcaca ttgttggcga ggatgtatgc ccacacgtcg tgttccgacc agtcgaaaat
    27961 cgggttgtat ttgccgatgc cccgtccggc atcgtattcg gcaaacggca gttccgtgcg
    28021 tgtggcggat tgttcgcggc gttgcccggt aagccaggcg tccgcgcctg caatggcgcg
    28081 gttgagcggt tcggtttttc ggatgcggca gcattcgcgg cgcgcttcaa cgctgtcgta
    28141 aaaggcaaac ctgcctttgc tttccacata acggtcggca tcttctcgaa ccggccggaa
    28201 acgctttatc cgcaaatggg gatatgcgcg tccgagcctg tccagcaggt tcagggtttc
    28261 cgtgtggagc agccccgtat ccaaggtaaa aatgccgata ttgaggtttt cgccggcgat
    28321 aaggtcggta atcaccatat cttctgccgc aaggctgctg gcaaaccgtg catcccggtg
    28381 gctgccgaca atccggtgca ggcgttgttt gagggtttcc gttttttccg caagggcggt
    28441 ttcgccgccg gatccgatat gcggtatctg ccacagggcg ggtttgaaca gtgtcgtttc
    28501 catttttccc gccttatgcc gcccgttgtc cggcattcag tccgcccaat gcgggatacg
    28561 tctgcccgac ccggttttct ccttcgccgt tttcaccgaa ccaggcgagt ttttcgtgca
    28621 gccccaccac ttcgcctatg acaatcaatg ccggattcgg cgcggtttcg gcgagttcgg
    28681 caaggttggc gagcgtgccg gttgcggttt tttgagccgg cagcgtgcct tggctgataa
    28741 cggctgccgg cgtgtcgggc gagcgtccgt gctgttgcag ccgttcggca atcagggcgg
    28801 ctttgagcgc acccatataa atcaccaagg tctggcggct gcgggcgagg gtctgccatt
    28861 cgatgtcggg cgcatccgcc ttgcggtggc cggttacgaa aaccgcactt tgggcataat
    28921 cgcggtgcgt gagcgggatg ccggcatagg cggtcgcgcc gacggcggcg gtaatgccgg
    28981 ggacgaccga aaacggaatc tgatggcgtg ccaaggtttc caattcttcg ccgccgcgtc
    29041 cgaacacgaa agggtctccg cctttcaaac gcaccacgcg cctgccttcg cgggccagcc
    29101 tgaccataag cgcattggtg tcctcttgcg gggtgcgctc gccccgggcg cgcttgccga
    29161 caaaaatccg ttccgcatcg cggcggacga gggacagtat gccgtctgaa accagcgcgt
    29221 cgtaaagcac cacgtctgcc tgctggattt cctgcagccc tttgagcgtc agcagccccg
    29281 catcgccggg acccgcgccg accagcgaga cggagccgcc ttgatcattt tgacgacttt
    29341 gttccaattg gcctgccaat tcccgttcgg caagggtgtt ttgccggttt ttgacgaggg
    29401 cggcgaaacg tccgttaaac tgcttttccc aaaagcggcg gcgttcggta acggatttca
    29461 gtttgccctt gacggcatcg cgccaccttc ctgaaatttc cgccatatcg cccaaagacg
    29521 gcggcagcag ggcttccagc ctttcacgca gcagtcgggc gaggacgggc gcgctgccgg
    29581 agctggaaac ggcaatctga accgggttgc ggtcgataac cgacgggaag atgaagctgc
    29641 aatggtcgcg gtcgtccacc acgttgaccg gcttttggca gctttcggca agatggaaaa
    29701 cgcgccggtt gagggcttgg tcgctgcttg ccgcaatgat gaggaaaacc gtgcggatgt
    29761 gttcggcacg aaattcttcg gcaagccaca ggattttgtt ttccgccgcc aacgcggaga
    29821 gttcggcatt caggtgtttt gcggcaaccc tgacctctgc gcccgccttc agcagcaggc
    29881 tgattttgcg tgcggcgacc gcgccgccgc ctacgaccaa tacggggcgg ccggcgaggt
    29941 tggcgaaaat agggaaataa ttcactggct gactcctttg ctgtttgccc gcaccttgtt
    30001 tccgatacgg tgcgtcgcgg catttttgtc ggaatgcggg tcattttaga caaaaggatt
    30061 ttccccggtt aaataataaa aaggtatttg ttagaagctg aaagctatat gggggcggct
    30121 gcggatgcgg cggttttccg ttttataacg gtttcggaag aaaaacggcc tgaagccgtt
    30181 tcgggcattc agaccgtttg cgtggtgagg ggatgccgtc cgaagggcga aaagggcttc
    30241 agacggcatt gatgtcgggt ttcaggacag gagcaggatg gcggctgcgg caagcgaggc
    30301 aaccgataat gcggcggcaa gcgcggcttt gcctgcaaag cggattgagg ttttgccttc
    30361 gatgtatttg aagccggtta tcatcgggag gatgaggttt ttctttttga atacgcggta
    30421 tgcggcgacg gcggcgatgt ggattgcaga aaaaacggcg agcagcttga aaaagttgag
    30481 gtggattttc cgcataaggc tgcccgtatg ttcggaaacc aaatggttga ggtagccgtt
    30541 ggtgctgaag gtgttttcat cggcggcaaa aagcccggtg ccgacttgga aggacacggc
    30601 ggccaaaagc gcaacgacca tcagtgcgcc caaggggttg tgtccgggct ggatgtgttc
    30661 gggaataccg tttttcagat agccgcgtat gcctgcccag ccttggacga aacgggaaaa
    30721 acgggcggta tcgctgcccc aaatgcccca gcagaggcga aatacgagca ggaaaaggac
    30781 gaacagcccg acgcgcgtgt gccattgcag catatcgccg ccggctttcg cgctatacca
    30841 cataaagggc agggacgcgg caagcagcca gtggaaaagg cgggtgggga ggtcccagac
    30901 tttggttttg tttttcataa tcggtttccg gcggtagaat cggtttgttt tcgagcctta
    30961 ttttaaccga ttggaggggc aatgtttccc gtttttcatc tttcaggcga gagccgccgc
    31021 cagatgcttc agacggcatt gcgttttccc catgttttca aagcccgtgc ggaagattcg
    31081 cacaaaggga ctttcggcac gctcgccgta gtcggcggat cggcagggat gagcggcgcg
    31141 cccgtattgg cggcatcggc ggcaatgtat ctcggctgcg gcaaagtgtg ggcgggtttc
    31201 aatcaggata cgctaccttt tgccgttatt gccggttttc ccgagattat gctggatacg
    31261 gcggacagtt tggccaaacg tcaagatata aacgcctggg ttgtcggttg tggattgggt
    31321 acaggtaggg cggcggtcgg aacgcttgcc ggaattttga cggaacacac ggacaagccc
    31381 gtcgttttgg atgcggatgc gctgaacata ttatcaaccg atgccgaaac ccgaaatctg
    31441 gcgcgcgggt gtaaaaacct gattttaacg ccacaccccg ccgaagccgc gcgcctgctt
    31501 ggaacgacgg ttgcgcaggt tcaggcggat cggacggcgg cagtgaggaa gataggggca
    31561 attttcggcg caaccgtggt tttaaagggg cacaaaacat tggttgcctc acccgatacg
    31621 gaaatctatg tcaacgaaag cggcaacgcg ggattggcaa cggcgggcag tggcgacgta
    31681 ttgggcggca tcatcggcag tctgctcgca cagggcgtgc cggtttttga agccgcctgc
    31741 gcgggcgcgt ggctgcacgg cgcggcggcg gatgtcataa aagaatcggc aggcattgcg
    31801 gcagggctgt tggcagggga aatcgctccg gcggcaaggt ggctgcgcaa ccggataact
    31861 aaaagtatgt aagaagatat agtggattaa caaaaaccag tac
//
LOCUS       DAWIVB010000005        30141 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWIVB010000005 DAWIVB010000000
VERSION     DAWIVB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30141
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.005"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(93..932)
                     /locus_tag="WIX60_000150"
     CDS             complement(93..932)
                     /locus_tag="WIX60_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ5304484.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="WIX60_000151"
     tRNA            1217..1292
                     /locus_tag="WIX60_000151"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1357..2550)
                     /locus_tag="WIX60_000152"
     CDS             complement(1357..2550)
                     /locus_tag="WIX60_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ5304485.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     NRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="WIX60_000153"
     CDS             complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="WIX60_000153"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ5304486.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAAAAQSPELKKRW
                     YAVEFLSTLSGEMLVTMIYHKRLDAEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACRTAEGLGSDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3806..4906
                     /gene="aroC"
                     /locus_tag="WIX60_000154"
     CDS             3806..4906
                     /gene="aroC"
                     /locus_tag="WIX60_000154"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ5304487.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIRVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRSAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4998..5423
                     /locus_tag="WIX60_000155"
     CDS             4998..5423
                     /locus_tag="WIX60_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ5304488.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETLSVEAEAAESSAAE"
     gene            complement(5497..7482)
                     /gene="parE"
                     /locus_tag="WIX60_000156"
     CDS             complement(5497..7482)
                     /gene="parE"
                     /locus_tag="WIX60_000156"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ5304489.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVHDNGRGIPVGLHPEEGVSVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDDHNGDFSIGEGAAFALTWLEE
                     SSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVAGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7549..8073)
                     /locus_tag="WIX60_000157"
     CDS             complement(7549..8073)
                     /locus_tag="WIX60_000157"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ5304490.1"
                     /translation="MLDREGYRPNVGIILINNRNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8239..9534
                     /gene="serS"
                     /locus_tag="WIX60_000158"
     CDS             8239..9534
                     /gene="serS"
                     /locus_tag="WIX60_000158"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ5304491.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSILDEGGLPMKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9612..10610)
                     /locus_tag="WIX60_000159"
     CDS             complement(9612..10610)
                     /locus_tag="WIX60_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ5304492.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGKYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10735..11811)
                     /gene="prfA"
                     /locus_tag="WIX60_000160"
     CDS             complement(10735..11811)
                     /gene="prfA"
                     /locus_tag="WIX60_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ5304493.1"
                     /translation="MKPSILEKLQQLSDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLEEITNALIAEHQAELLAAMGD"
     gene            complement(11916..12686)
                     /locus_tag="WIX60_000161"
     CDS             complement(11916..12686)
                     /locus_tag="WIX60_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ5304494.1"
                     /translation="MNFTDTHCHLADPALRENLPHILTAARKVGVKRFIVPATRPQDW
                     QDVADLAEMPSEHGQIRIALGIHPWFSDGIAERDFVRLETMLAHYPQAWVGEIGLDFY
                     DKTQTSQQRERQIQVFVRQLEIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12694..13686)
                     /locus_tag="WIX60_000162"
     CDS             complement(12694..13686)
                     /locus_tag="WIX60_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ5304495.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVSQALSPFSDGLDFE
                     WHVCNPLIDSSSVTLQHWRDWLDIIADKLPSCDGILILHGTDSMAYTANLLALALQGL
                     GKPIVLTGSQWPYAAENSDAPRNLSTAVAAFSLKLKQTVIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGTLAEWDETRGWHNLRLPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVRDFARQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEDVYKELVRLKLV"
     gene            complement(13968..14651)
                     /locus_tag="WIX60_000163"
     CDS             complement(13968..14651)
                     /locus_tag="WIX60_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ5304496.1"
                     /translation="MFAFLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILRFKPIARIMTPKRYEQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFIIMDGLAALISVPIWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFVILGIGATVVAWIWWKKRQRIQFYRSKLKEKRAQRKAAKA
                     AKKAAQSKQ"
     gene            complement(14842..16176)
                     /gene="glmM"
                     /locus_tag="WIX60_000164"
     CDS             complement(14842..16176)
                     /gene="glmM"
                     /locus_tag="WIX60_000164"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ5304497.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVVDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQQWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWAREGAERIAAAIGGI"
     gene            complement(16306..17157)
                     /gene="folP"
                     /locus_tag="WIX60_000165"
     CDS             complement(16306..17157)
                     /gene="folP"
                     /locus_tag="WIX60_000165"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ5304498.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSSEEEWARVEPVLVEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGALELLACQADTGICLMHMRGLPENMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMVGELTGETDAAARVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(17297..18190)
                     /locus_tag="WIX60_000166"
     CDS             complement(17297..18190)
                     /locus_tag="WIX60_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ5304499.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHDAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGNLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLNHTKGHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18281..20395
                     /locus_tag="WIX60_000167"
     CDS             18281..20395
                     /locus_tag="WIX60_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ5304500.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALR
                     NNSIKIETVNGAFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20459..21937)
                     /gene="ubiD"
                     /locus_tag="WIX60_000168"
     CDS             complement(20459..21937)
                     /gene="ubiD"
                     /locus_tag="WIX60_000168"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ5304501.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPISPYLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21957..22472)
                     /locus_tag="WIX60_000169"
     CDS             complement(21957..22472)
                     /locus_tag="WIX60_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ5304502.1"
                     /translation="MYTCTSCGENHEEMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSASEKSFNDYQSRFYDNLENAVYF
                     GMICNQLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGGEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22479..22958)
                     /locus_tag="WIX60_000170"
     CDS             complement(22479..22958)
                     /locus_tag="WIX60_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304503.1"
                     /translation="MYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAALFITLFFA
                     AVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRR
                     VTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(23193..23438)
                     /locus_tag="WIX60_000171"
     CDS             complement(23193..23438)
                     /locus_tag="WIX60_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ5304504.1"
                     /translation="MTEQEIYRLLRDTLTELFEIEPERITPDTNLYEDLEIDSIDAID
                     LIDRIKRETGRKLQAEDFRNVRTVNDVVQAVLKIQAG"
     gene            complement(23450..23710)
                     /locus_tag="WIX60_000172"
     CDS             complement(23450..23710)
                     /locus_tag="WIX60_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ5304505.1"
                     /translation="MNDLENQIKQLIIDSLALEDITAADIGSEDALFGDGGLGLDSVD
                     ALELGLAVQKHFGFRLDGEQENLREHFANVKTLAAFVKSRQA"
     gene            complement(23707..24465)
                     /locus_tag="WIX60_000173"
     CDS             complement(23707..24465)
                     /locus_tag="WIX60_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ5304506.1"
                     /translation="MDKLDYCRRFFATWLGFVIFGVGGIMMKLVLLPYTLNGTSGSVA
                     RQLAARRIIGTSWRLFVAYLKWSGVLEVSFKGVEKLNRPGQLILANHPSLLDVVLLVG
                     HVPEMNCIVKKDLQHNPAMSSQIKGAGYIPNEESEAMLETVKAVFDSGQSLLVFPEGT
                     RTGWDGRVKMHRGAVSLGLRYAEVITPVCIKMNPPNFKKGQPWYRIPPKRIRYEITVG
                     DDILPQDWLAEKPLPIAARRLNEYLQDYFTRKTI"
     gene            complement(24458..25213)
                     /locus_tag="WIX60_000174"
     CDS             complement(24458..25213)
                     /locus_tag="WIX60_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEZ5304507.1"
                     /translation="MPSIICRFHFDIAAWRVSGSKMRDMAQWAKWAECPDFADGLPDV
                     RPELPFLPAMQRRRLSKAARLVCDAAWDIASAHPGSPVVYASHDGEMARSFDLWLELL
                     KSHTVSPTSFGLSVHNATAGQWSILRQDMSEQTALAVCADGVETALAEAASLLEEGCG
                     SVLVLAADDPLPEGYAVSATRAPFAYALAMVLTKGTRYSLTLSASDDMPSEAGMLPEA
                     YWSGLEWVRFLLNGSRECRRVYRNREWLWQRNG"
     gene            25368..25901
                     /locus_tag="WIX60_000175"
     CDS             25368..25901
                     /locus_tag="WIX60_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
                     /protein_id="HEZ5304508.1"
                     /translation="MPVFASLPPQYMDTLPECPITNTASLLPHSGRMVLIDRITRYGD
                     DFVEAGAQVSPNHILLLDDKLPYTAFIELMAQAVGAYAGIQARKNARSVRLGFLLGTR
                     KLEIFAQSVPIGTHLLATAHMSIQDAGGMGVFDCELRWTDAPETSSETLPSDGILARA
                     SLNVYSPEHPAGTTDAV"
     gene            25929..26657
                     /gene="fabG"
                     /locus_tag="WIX60_000176"
     CDS             25929..26657
                     /gene="fabG"
                     /locus_tag="WIX60_000176"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ5304509.1"
                     /translation="MTETVLITGSNRGIGKAVAFGLAEDGFDIAVHCRSRRDEAEAVA
                     EEIRALGRNARVLQFDVSDREACREILTADIEANGAYYGVVLNAGLTRDNTFPAFSDD
                     DWDVVLRTNLDGFYNVLHPLVMPMIRRRKAGRIVCMASVSGLTGNRGQVNYSASKAGI
                     IGAAKALAVELAKRKITVNCVAPGLIDTDIIDENVPVEEILKAVPAALMGLPEEVAHA
                     VRFLMDEKAAYITRQVIAVNGGLC"
     gene            26651..27901
                     /locus_tag="WIX60_000177"
                     /pseudo
     CDS             26651..27901
                     /locus_tag="WIX60_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257271.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            27955..28030
                     /locus_tag="WIX60_000178"
     tRNA            27955..28030
                     /locus_tag="WIX60_000178"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27988..27990,aa:Ile,seq:cat)
     gene            28105..28863
                     /locus_tag="WIX60_000179"
     CDS             28105..28863
                     /locus_tag="WIX60_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ5304510.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAKKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            28864..29928
                     /locus_tag="WIX60_000180"
     CDS             28864..29928
                     /locus_tag="WIX60_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ5304511.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRDCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAAH
                     QCVAARFTINHTADKLLDAI"
BASE COUNT         6969 a         8498 c         7823 g         6851 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttagaatt ttgcaccgga ttggtttgcc
      121 atatagtcaa cagccgcttt gacttcgtca tcgctcaaac cggcattgcc gcctttggcc
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac tatcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga caggctggat acgggtctgc
      781 gttgctgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tgttgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaacaat cctttgaaaa taatggttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttgcg ttttccgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gagaccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttat ttcaagactt ccgcaaatgc
     1381 gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg acgcacatcc tgccggaatc
     1441 cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact tgttccacgc tcaatcctgt
     1501 gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa tcgcgattgg ggatttgcgc
     1561 agttaagaca tcataaagtt tctgccgcat cgcacggatg cggtcgcgca tcatataaac
     1621 ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc acgtcggcgg cgatatacgc
     1681 gccgtgcgcg ggcgggctgg agtagatgcg gcggacggtg aatttgagct gtccgaacac
     1741 caaatccgct tcttccttat tcgggcaaac cacgcttaag ccgccgacgc gctcgccgta
     1801 gagcgacagg ttttttgaga aggaattgct gacgaacaag ggcaattcca tttccaccgc
     1861 tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg aatccttggt aggcaatgtc
     1921 cataaacgga atcagtttgc gcgttttgat gatgtgcaac acttcgtccc attgccgttc
     1981 cgacatatcc acgccggtcg ggttgtggca gcagggatgg aggatcagga cgctgttttc
     2041 gggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc acgccgacag tggcagggtc
     2101 gtaatatggg taagtgccga cctcgaaacc tgcgccttca aaaatgccgc gatggttgtc
     2161 ccaagtcggg tcgctgacgt aggcgcgcgc ttcgggaaac cagcggtgca ggaagtccgc
     2221 cccgactttg agcgcgcccg agccgcccaa ggtttgtacg gtaacgatgc gcccttgcgc
     2281 aagcgcggga ttgtctttgc cgaacaataa atgctgcacc gcgctgcggt aagtgtccaa
     2341 gccttccatc ggcaggtagg gcgacggcgc aggcgcggcg gcacgggcgg tttcggctcg
     2401 gctcacggat tccaacacgg gcattttgcc ttcgtcgtcg aaataaatgc ctatgctcaa
     2461 attgactttt tcggggcgcg ggtcgttttt gaaggtttcg accaaactca aaatcgggtc
     2521 gccaggatag tattcgatgt gtcggtacat agtccttacc tcttgctttt tcaaaggatt
     2581 ttctttttca acaatacacc actttcgata tggtgcgtaa acgggaattg gtcgaacagg
     2641 gcggcacgtt cgaccgcatg ggtttccgcc aaggtgtcca aattggcgcg caacgtttcg
     2701 ggattgcagg aaatgtagat gatgttgtca aactgcgaca ccagcttcaa agtttcctca
     2761 tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa atgcgtaatc cgtcaaagca
     2821 ataccgccat ccttaaggcg tttaaactca cgttttccgg tataggcttc ggtaaattct
     2881 tcagcagaca gacgggcgat tttgatgttg ccgatgcggt tggcttcgat attccattgc
     2941 gccgcgctga cggaggtttt ggagatttcg gttgccaaaa cctgtcggaa atatcgggac
     3001 agcggcaggg tgaaattgcc gtttccgcaa tacagttcga gcaggtcgct gcccaagcct
     3061 tccgccgtgc ggcacgccca ttcaagcatt ttctgacaca cggcggcatt cggttgggta
     3121 aaactgcctt caatttgccg ataacggaaa tcccggttgc cgaccttcaa agtttccgtt
     3181 acatagtcct gttttaagac tattttctgt cccctgctcc gcccaataac ggaaatatcc
     3241 aactgttgct gtaacgcttg cgccgcctgc atccactcag catcaagcct tttgtggtaa
     3301 atcatggtaa ccagcatttc cccgctgagc gtggacagaa attcgacggc ataccagcgt
     3361 tttttgagtt cgggggattg cgcggcggcg gcgatcagct cgggcatgag gcggttgaca
     3421 gcctcggaag ctgcttcaaa acggtcgcag cgtatcatgc ttgcgccgct ggctttctgc
     3481 cctttttcaa acatggcata aaacatttcc ccgccttcgt gccaaatacg gaactcggca
     3541 cgcatacggt aatgtttgtc cggagattcg tacacttccc actcaggaac atccaaacct
     3601 gcaaaaaggg ttttaaggta gtctttttta ccttgaagct gctgcgtgta gtcattcata
     3661 tcgctatcct gaaaaatcag accggattat aagccgattt gcctcgccgc acgcaaactc
     3721 tttgactgcc gccccttcaa tgacggacgg gcttttgtgc taaaatccgc catctttcca
     3781 cactataccg ataaagggaa aaatcatggc aggcaacact ttcggacaac tcttcaccgt
     3841 taccaccttc ggcgaaagcc acggcgcggg tttgggctgt atcatcgacg gctgcccgcc
     3901 cggcttggaa ttaagcgaag cggatatcca atttgacctc gaccgacgca aacccggcac
     3961 cagccgccac gttacccaac gccgcgaagc cgaccaagtc gaaatcctct ccggcgtatt
     4021 cgaaggcaaa accaccggca cgcccatcgc cctcttaatc cgcaataccg accagcgcag
     4081 caaagactac ggcaacatcg ccaccagctt ccgccccggc cacgccgact atacctattg
     4141 gcacaaatac ggcacgcgcg actaccgcgg cggcggcagg agctccgccc gtgaaaccgc
     4201 cgcccgcgtt gctgccggag ccgttgccaa aaaatggttg aaagaaaaat tcggcacgga
     4261 aatcaccgcc tacgttaccc aagtcggcga aaaagaaatc cggtttgaag gctgcgaaca
     4321 catttcccaa aatccttttt ttgccgccaa ccatagccaa attgccgagc tggaaaacta
     4381 tatggacagc gtgcgcaaat ccttggattc cgtcggcgcg aagctgcata tcgaagcagc
     4441 caatgtccct gtcggcttgg gcgaacctgt ttttgaccgc ctcgatgccg aaatcgccta
     4501 cgcgatgatg ggcatcaacg ccgtcaaagg cgtggaaatc ggcgcaggtt ttgacagcgt
     4561 aacgcaacgc ggcagcgaac acggcgacga actgaccccg caaggcttcc tgtccaacca
     4621 ctcaggcggc atcctcggcg gcatcagcac cgggcaagac atccgcgtca atatcgccat
     4681 caaacccacc agctccatcg ccaccccgcg ccgcagtatc gacatcaacg gcaaccccat
     4741 cgaactcgcc acgcacggca ggcacgaccc ctgcgtcgga ctgcgctccg cgccgatcgc
     4801 cgaagccatg ctcgcgttag tcctcatcga ccacgccctg cgccatcgcg cgcaaaatgc
     4861 cgacgttcag gttaatacgc ccgacattac cctttcaaac aaataaaaat ttagccaaaa
     4921 cacagacttt ataatagaat atcgagcatt gccgtgcagc cttgacgcac gggtttgttt
     4981 agaggaatac aaccgaaatg acacaagaaa ccgctttggg cgcggcacta aaatccgccg
     5041 tccaaactat gagcaaaaag aaacagaccg aaatgatcgc cgaccacatc tacggcaaat
     5101 acgatgtatt caaacgcttc aaaccgttgg cgctcggcat cgatcaggat ttgattgccg
     5161 ctttgccgca atacgatgcc gcactgattg cacgcgtcct cgccaaccac tgccgccgtc
     5221 cgcgctatct gaaagccttg gcgcgcggag gcaaacgttt cgatttgaac aaccgtttca
     5281 aaggcgaagt tacccccgaa gaacaggcga tcgcgcaaaa ccatcctttt gtgcagcagg
     5341 ctttgcaaca gcagtccgcc caagctgccg ccgaaacgct gtctgttgaa gccgaagcag
     5401 ccgaatcttc cgcagcagaa taaatcccca aacgaaatgc cgtctgaaaa ccgatttggt
     5461 ttcagacggc attttttcgt atgcggcaat cacggttcaa atatccaatt ccgccgtatc
     5521 gccttcgcgt tccatccaag cgcggcgggc ggcggcttcg cctttgccca tcagtttgac
     5581 gaagatgtcg cgcgtctcgt catctgcacc ttctgggatt tgtacctgca acaggcggcg
     5641 ggtgtcgggg tgcatggtag tatctttgag ctggtcgggg ttcatctcgc ccaagccttt
     5701 gaaacggctg atggaatagg cggtttcttt aacgccttct ttttgcagcc gctccaaaat
     5761 gctgtcgagt tcgttttggt cgagggcgta gaatttgcgg gcaggtttgc tcttaccttg
     5821 tgcgttgaca tcgacgcgga acagcggcgg ctgggcgacg tagatgtgtc cgtcggcaac
     5881 cagtttcggg aagtggcggt agaacagggt cagcagcaaa acttgaatat gcgagccgtc
     5941 cacgtcggca tcggacagga tggcgatttt gccgtagcgc aggccgctta aatcgggatg
     6001 gtcgttaata ccgtgcggat cgacgccgat ggcgacggaa atgtcgtgga tttcggcgtt
     6061 gccgaagagt tggtcggggt ggacttcaaa gctgttgagc actttgccgc gcaggggcag
     6121 gatggcttgg gtggctttgt cgcgggcgag tttggctgag ccgccggcgg aatcgccttc
     6181 gacaaggaag agttcgtttt cgcggatgtc ttcgctttcg cagtcggtca gcttgccggg
     6241 caggacggcg acgccgctgc cttttttctt ttcgattttc ttaaccgaac gcatccgcgc
     6301 ctgcgcctga cggatggcga gttcggcaat ttttttaccg aagtccacgt tttggttcag
     6361 ccacaattcc aaagggtcgc ccgctacagc ggcgacaagt ttcagcgcgt cgcggttggt
     6421 cagcttgtct ttggtctgac cttggaactg cgggtcgagg acacgggcag agaggacaaa
     6481 ggcggttttg ccgaacacgt cgtcgctttg tactttcacg ccgcgcggca agaggttgtg
     6541 cagattgatg aaattgttga cggcgttgaa cacggcttgt ttcaagcccg cttcgtgcgt
     6601 gccgcccagc ggggtgggga tgaggttgac ataactttcg ttggcacacg agctttcttc
     6661 cagccaagtc agggcaaacg cggcgccttc gccgatgctg aaatcgccgt tgtggtcgtc
     6721 tgaaatgtag ttttcgcaag agaagatagg cacggcttcc tgcgcgtcgg caatcaggtc
     6781 ggtcagatag cttttcagac cgtcggggta atgccaggtt tgggtgtgcg cttcgtcttc
     6841 gcctttgacc ggacgggtca gggaaacgcg cacgcccggc agcagcacgg ctttggcgcg
     6901 cagcaggcgt tcgagttcgg ggatgctgta attcgggctt tcaaaatatt tgccgtccgg
     6961 ccagacgcgc acttcggtgc cgctgtcttt gacggcgcat ttgcccactt gtgccaacgg
     7021 ttcgaccacg tcgccgccgg caaacacgat gcggtggatt ttgccttcgc gtttgaccgt
     7081 tacttcaagg cgggtggaaa gggcgttggt gacggatacg cccacgccgt gcaggccgcc
     7141 tgaaaaggca tacgcgctgc cgccgtcttt tttgttgaac ttgccgcccg cgtgcagacg
     7201 ggtgaatacg agttcgacta cggatacgcc ttcttcggga tgcaggccga cgggaatgcc
     7261 gcgcccattg tcgtgcacgg aaagcgaacc gtcttcatga atttgcacgt cgattgcagt
     7321 cgcgaaaccg cccaacgctt catccgccgc gttgtcgatg acttcttggc agatatgggt
     7381 cgggctgtca gtgcgggtgt acatgccggg acgttctttg accggctcca agcctttgag
     7441 gacggtgatg ctggattcgc tgtattggtt gtttttagcc ataggaataa tctgaaagta
     7501 agaaaaacaa cgctttcaga cggcctgaaa gcgttgcgtt ccgttgtttt accggttgcc
     7561 ggaaggttgg cgggcggcaa agtcttcata actttccata ccgcgcagga agcgggaaga
     7621 gagttctttc aacgccccca aataaacgtc gcgtttgaac tcgatgactt cgtcaacggg
     7681 cgcccaatat tgatgccaac gccagccgtc aaattcgggg tggcgggtgg cgcgcaggtt
     7741 gacatcgcaa tcccggccgg tcaggcgcag gagataccaa atctgcttct gcccgcgata
     7801 agagccgcgc cattcgcggc gcacccagtt gttcggcacg tcgtaacgca gccagtcgcg
     7861 cgtccgcccg acgattttga cgtgttgcgg cagaagtccg acttcttcgt aaagctcgcg
     7921 gtacatcgcg gtttcggggc tttcgcccgg cttgatgccg ccttgcggaa actgccaaga
     7981 atgttcgcgc acgcgcttac cccaaaagac ttcgttgcgg ttgttgatta agatgatacc
     8041 gacattgggg cgatagcctt ctctgtccaa cacggtgtcg ccctccgtta aattcaatct
     8101 tgggattttc ccacaaatcg aacggttttg acaaatcaga cggtatggtt caagcatgtc
     8161 gaaacacgga cggattcggg aaaatatctt aaatttggtt tacaataatg tgtttcaaat
     8221 taattcggga atcagaccat gttagatatc caattgctcc gcagcaacac cgccgccgtt
     8281 gccgaacggc ttgcacggcg cggttatgac tttgataccg cacgttttga cgcactggaa
     8341 gaacgacgca agtccgttca ggtgaaaact gaagaactac aagcctcgcg caacagcatt
     8401 tccaaacaaa tcggcgcact gaaaggtcag ggcaaacatg aagaagcgca ggcggcgatg
     8461 gatcaagtcg cccaaatcaa aaccgatttg gaacaggctg ccgccgattt ggatgccgtt
     8521 caaaaagaat tggacgcatg gttgttgagc atacctaacc tgccgcacga aagcgtacct
     8581 gccggtaaag acgaaaccga aaacgtcgaa gtccgcaaag tcggcacccc gcgcgaattt
     8641 gactttgaaa tcaaagacca tgtcgatttg ggcgaacctt tgggtttgga tttcgaaggc
     8701 ggcgcaaaac tctccggcgc acgatttacc gttatgcgcg gacaaatcgc ccgtctgcac
     8761 cgcgcgctgg cacaattcat gctcgatacg cacacactga aacacggcta caccgagcat
     8821 tacacgccct atattgtgga cgacaccacc ctgcaaggta cgggccagct gcctaaattt
     8881 gcagaagatt tgttccacgt tacccgtggc ggcgacgaaa ccaaaaccac gcaatatctg
     8941 attccgacag ccgaagttac cctgaccaac accgttgcag gcagtatttt ggacgaagga
     9001 ggactgccga tgaagctgac cgcgcattcg ccctgtttcc gcagcgaggc gggttcgtac
     9061 ggcaaagaca cgcgcggtct gattcgccag caccagttcg acaaagtgga aatggttcaa
     9121 atcgttcatc ccgaaaaatc atacgaaacg ctggaagaaa tggtcggcca tgccgaaaac
     9181 atcctgaagg ctttggaact gccctaccgc gtgattaccc tgtgtaccgg cgacatgggc
     9241 ttcggcgcgg caaaaacgta tgacttggaa gtttgggtgc cggcgcaaaa tacctaccgc
     9301 gaaatctcaa gctgctccaa ctgcgaagat ttccaagccc gccgcctgaa ggcgcgtttc
     9361 aaagacgaaa acggcaaaaa ccgcttggta catactttga acggctccgg cttggctgtc
     9421 ggcagaacgc tggtcgccgt attggaaaac catcaaaacg ccgacggcag catcaacatc
     9481 cctgccgcac tgcaaccgta tatgggcggt gttgccaagt tggaagtcaa ataagtttgc
     9541 aggctgcctg aacgtcaaat gccgtctgaa acctgtttca gacggcattt cctttaaact
     9601 tttaaaacac gtcagccgtc ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc
     9661 tcgcggatat tggacagcgt aacttccgaa atattaccca acgcctcttc cgtcaaaaat
     9721 gcctgatggc cggtaaacag cacattatga caagacgaca ggcggcggaa cacgtcgtcg
     9781 gtaatcacat cgttggattt gtcttcaaaa aacagctcgc gctcgttctc gtacacatcc
     9841 atgcccaatg cgccgatttt ccggcgtttc aacgcctcaa tcgcggcggc actgtcaatc
     9901 agcccgcccc ggctggtgtt gataatcatc acgccgtctt tcattttgtc gaacgccgct
     9961 tcgttcagca tatagtggtt ttccggcgtg gcggggcaat gcagcgtgat gatgtccgac
    10021 cgggcataca gctcgtctaa atccacatat ttgccgccga ttttttccgc ttcggggttg
    10081 caaaacggat cgtaagccag caggttcatg ccgaaaccct ttaaaatccg catggttgca
    10141 ataccgattt tccccgtgcc gataacgccc gccgttttgc cgtacatatt gaaaccggtc
    10201 agaccttcca gcgaaaaatt cgcatcgcgg gtacgctgat aggctttgtg gatacggcgg
    10261 ttcaacgtca gcatcagacc gaccgtatgt tccgcaaccg attcgggcga ataggcaggc
    10321 acgcgcacga ctttcaagcc caactcttca gccgccttta aatccacatt attgaagccg
    10381 gcacaacgca acgccacagt tttcacgcca atttgcgcca atttttccaa cacgggccgg
    10441 ctgccgtcgt cgtttacaaa aatacagacc gcttccgcgc cttccgccat tttcgccgtt
    10501 ttcgcatcca gcataaaatc aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg
    10561 gtaaaatgtt cgcggtcata gcttttcgta ccgtaaatcg caatcttcat caatatgtcc
    10621 agttgtcgtc tatggttgag aaacggcata atacactgaa ttcaaacaaa tcagtagaat
    10681 atggtggatt aaaattgatt gcatgcacgg catttccatt tcaaaacaca aaactcaatc
    10741 gcccattgcc gccagaagct cggcctgatg ctcggcaatc agggcattgg tgatttcttc
    10801 caagtcgccg tccatcacaa aatccagctt gtgcagggta aggttgatgc ggtggtcggt
    10861 tacgcggcct tggggatagt tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat
    10921 gagggatttg cgctcggcgg cttctttggc ttgggcttcg cgtttttgcg cgtcgttcag
    10981 gcgggcggcg aggactttca ttgcctgcgc tttgttggca tgttggctgc ggccgtcttg
    11041 gcattcgacc accatgccgg tgggcaggtg ggtgatgcgg acggcggagt cggttttgtt
    11101 gatgtgctga ccgcccgcgc cggatgcgcg gaaggtgtcg atgcgcaggt cggctgggtt
    11161 cagttcgatg tcttccagtt cgtccgcttc gggcatgacg gcaacggtgc aggcggaggt
    11221 gtggatgcgg ccttggcttt cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc
    11281 aaatttcaaa cggctgtacg ccccgagtcc gacaatacgg gcgatgactt ctttatagcc
    11341 gcccaattcg ctttcgttgg cggacacgat ttcaacctgc caacggttgc gctcggcgta
    11401 gcggctgtac atacgcagca aatcgccggc aaacagcgcg gcttcgtcgc cgcccgttcc
    11461 ggcgcgtatt tcgatgaaga tgtttttgtc gtcgtcggca tctttgggca gcagcagttt
    11521 ttgcagttcg gtatcgagtt cgccgatttt ggctttggcc gcttcgattt cttcggcggc
    11581 aaagtctttc atttcggggt cggacaacat ttcttcggca tccgccaagt cgctttgggc
    11641 aagccgatag ttttggaaca cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag
    11701 cttgcggtag ttgtccatgt cggacgtggc ttcgggctgt ccgagaaggt gggtaacttc
    11761 ttccagtcgg tcgctgagtt gttgtagttt ttctaagata gacggcttca taattcttcc
    11821 ataacaaacg ccgcctgaat gttcagacgg catcaacact ggattattat aataggtttt
    11881 ccggatattc aaaaagataa tcttagatgg ataacctacc gtcccaacag ggcatcggca
    11941 ttgcgctccg ttacctttgc aatctcttct acacaggttc cgcggatttc cgcagcaatc
    12001 tttgcaatac ccggaatatt ggcaggcgta ttaatctctt ttttcagcat aaacgggcta
    12061 tccgtttcca atacgaaatc cccgtcgttc aaggctttaa gcgtatcgcg cactttacgc
    12121 gcgttcggat tgagcagcag cgaaccgatg ccgattttga aacccagttt cgtcaacaca
    12181 cgcgcttctt ccgcgctgcc ggagaaggcg tgaacgatgc cgccttgggc aaagcctgtc
    12241 tgtttgacgg cggcggcgat gtcggcggtg gctttgagat tatggataat cacgcggcgg
    12301 cgcagggttt gcgcaatttc aagctggcgg acgaaaactt gaatttgccg ttcgcgctgc
    12361 tgcgacgttt gggttttatc gtaaaaatcc aagccgattt cgccgaccca tgcctgcgga
    12421 taatgtgcca acatcgtttc caggcggacg aaatcccgct cggcaatgcc gtctgaaaac
    12481 caaggatgaa tgcccagtgc aatacggatt tgaccgtgtt cggacggcat ttccgccaaa
    12541 tccgccacgt cctgccaatc ctgcgggcgc gtcgcgggaa cgataaaccg cttcacccca
    12601 actttccgcg ctgcggtcag gatgtgcggc aggttttcgc gcagggcggg atcagcgaga
    12661 tggcagtggg tgtcggtgaa gttcatttcg atttcacact aactttagtc ttaccaattc
    12721 tttgtaaaca tcttccttac cccagccttg cgatacggcg agggtcatca gcgcggtggc
    12781 ggtttcgagg ttgcatttgc cgccgttgat gatgcccgag ttgcggaacg cgttgccttg
    12841 cgcgtaaacg gcggcggttt tgccttgtcg gacttggctg atgttgagca gcagtttgcc
    12901 ctgccgcgcg aagtctcgga cggcgcggat aaaaccttcg tctgcgggcg tgttgccgtg
    12961 tccgtagctt tgcaggataa gagcttgggc gggaagctgt ccgagtccgt ccgcaagttc
    13021 ttggacggca aagccgggga taagcgtgcg gacagcgatt tttgcctgcg ggtcgggata
    13081 acggattttg aggccgtctg aaacggctgc tgcgtcttgg gacgggaggc gaagattgtg
    13141 ccaaccccgg gtttcgtccc attcggcaag cgtgccgaaa tgcggattgt cgaagcctgc
    13201 agcagtttcg gtgctgactt tgctgctgcc gacggcggga tacagtttgc cgtcaaacgc
    13261 gatgacggtt tgtttgagct tgaggctgaa ggcggcaacg gcggtggaga ggttgcgcgg
    13321 ggcatcgctg ttttcggcgg cgtaaggcca ttgggaacca gtcaggacaa tcggtttgcc
    13381 caaaccttgc agagcgagcg cgaggagatt ggcggtgtac gccatgctgt ccgtgccgtg
    13441 cagtatcagg atgccgtcgc atgaagggag tttgtcggca atgatgtcca gccaatcgcg
    13501 ccagtgttgc agcgtaacgg aggaggaatc aatcaaggga ttgcagacgt gccactcgaa
    13561 atcgaggccg tctgaaaagg gggaaagggc ttggctaacc agtgcggtat cggggcgcag
    13621 gccttcgctg ctttgggtca tgcctatggt gccgcctgtg tagaggacga agattttttg
    13681 tttcatggac atcatcgggt cgtctgaaaa taataatacg gcttatttaa ctatatttcg
    13741 gacagactgg caatttggcg gcgcggacgg ttttcagacg gccttcaaat gaaaaagcac
    13801 ccgagggctg tcgatatttg attttccaag tagattttta ttcacgaaat aggagagccg
    13861 caacaagctt aaatcccttg tgaggttccc aacacggaag ataccgcttt gtggattaaa
    13921 aaatacggaa actattgaat atcgacaacc tatttaggtg cttgatttta ttgtttgctt
    13981 tgcgcggctt ttttggctgc cttggcggct ttgcgttgcg cccgcttttc tttcaatttg
    14041 ctgcggtaaa actggatacg ttggcgtttt ttccaccaaa tccaagcgac aacggtcgca
    14101 cctataccca agataacaaa aatacccgat tgcaggctgt gcattttcgc catcagccaa
    14161 tcgatgttgt gcgcaccgta ttcgcccaga taaatccaaa tagggacgga aatcagtgcg
    14221 gccagtccat ccataatgat aaaacgcaag tatgaaacct tgcggctgat accggctgta
    14281 acaaatacgg ccgttctcaa accgggcagg aaacgggcga caaataagac ccagttaccg
    14341 tatttgtcga atttttcctg aacctgctca taacgtttcg gcgtcatgat gcgcgcaata
    14401 ggtttgaacc ttaggatttt ctgcccccaa attcgtccgg cggcgaacat gatgccgtcc
    14461 ccgaccaata cgccgagcat accgactgca aacataatat gcggattggt ataacccata
    14521 cccgaaatca cgccgcctgt taccaaggtc aaatcctcgg gaatcggcac gccgaaaccg
    14581 cagatgacca atacaaaaaa aacagccgca taaccgtatt cgacaaaaaa ggcttctaaa
    14641 aaagcaaaca tggcggatat tccattgtcg gagataaaaa gtcagaacaa accgaaacat
    14701 tttctacatg aagcaggcat tctatcaaag attatgccgt ctgaaagcgg aaaaaaggca
    14761 gattcgcgca tcctccgcat gttcagacgg catttaaaca gaaaaccggc cggttctaaa
    14821 aacggttttg cctgattttg cctaaatgcc gccgatggcg gcggcaatgc gttccgcccc
    14881 ttcgcgcgcc caatccgcct gccgcgcctc caccatcacg cgcacgacgg gttcggttcc
    14941 cgaagcgcgc aacacgacac gccctttgcc ttcgagttct ttttccactt ccgccaacac
    15001 gtctttcgaa gcttcctgcc attgctgacc tttttggatg cgcacgttaa tcatcgtttg
    15061 cggatacggc tgccaatcgg cgcaaacggt ggcgaggtct tggttcagcg tttgcagtgc
    15121 cgccaaaact tgcagcgcgg aaataatgcc gtcgccggtg ttgtgtttgt ccatacacaa
    15181 aatatggccg ctggcttcgc cgccgatgag ccagccgcgt tggttcagct gttccaacac
    15241 atagcggtcg ccgactttgg cgcggcagaa atccacgccc tgctctttca gggcgatttc
    15301 catcgccata ttggtcatga ccgtgccgac cacgccgccg atgttgatac cttctcgggc
    15361 gcgggctttg gcaatgacgt aaatcaggct gtcgccgtcg taaacctgcc cgtttttatc
    15421 gaccatcatc aggcggtcgc cgtcgccgtc taaggcgatg ccgtagtcgg cttcatgctg
    15481 taaaacggcg gcctggagtg tcttggtata agtcgcaccg catttttcgt tgatgttgta
    15541 gccgttgggt tcgttgccga tgctgacgac ctgtgcgccc agttcgtgaa acaccttggg
    15601 ggcgacaccg taccccgcgc cgttggcggt atcgacaacc aacttcaaac cccgaaggtc
    15661 ggaatggctg ggaaaggtgg atttgcaaaa ttcgatatag cggtcgtccg caccgctgat
    15721 gcggcgtgcg cgaccgagac gggcggacgg ttgggttttc atttcgccgt cgattttggc
    15781 ttcgatttcc aactcgactt catcggaaag tttcacgccg ccttcggcga agaatttgat
    15841 gccgttgtcg gaataggcgt tgtgcgacgc ggaaatcatc acgccggcgg acaggcgcaa
    15901 cgcgcgggtc agataagcca cgccgggcgt gggcagcggt ccggtctgta ccacattcac
    15961 acccgccgcc gtaaaaccgg ccaccaaagc ggcttccagc atatagccgg aaatgcgcgt
    16021 gtctttgccg atgaggacgg tcggtttctg gtcggtgtcg tgctgcacca aaacctgccc
    16081 cgccgcatag ccgagtttca atacgaaatc gggcgtaatc ggaaattgcc ccacttcgcc
    16141 gcgcacgccg tccgtgccga aatatttttt tgccatgtgt tgctccgaga atgtggaccg
    16201 ttgtccgaga ttatacagtc agtttgtgcc ttgctgtctg caccgttgat gccgtctgaa
    16261 accaccccgc ccttttcaga cggcatgaag tatgcgaacc gctgtttaca gattgatgcc
    16321 caacgcttcc cacaccttca acgcgtccgc cgtcgccttc acatcatgca cccgcacgat
    16381 ttgtgcgccg cgcgccacgg aagccagcgc cgccgccacg ctgccgtgta cgcgcgccgc
    16441 cgcgtcggtt tcgcccgtca gttcgcctac catgcgtttg cgcgatacgc cgatcagcag
    16501 cggaaagcct gtttccgcca tcaattcggg caaatgcctc atcagtgcga tattgtgttg
    16561 caaggttttg ccgaagccga aaccggggtc gagtgtaatg cgttgcgggg cgatgcctgc
    16621 cgcgatgcat tccgccgccc gcgctttcaa ataccgcgcc acttcaccga caacatcctg
    16681 atatttcgga ttaatctgca tattctcagg caagccccgc atgtgcatca ggcaaatgcc
    16741 cgtatccgcc tgacacgcca gcaattcgag cgcgccttcg tcggtcaacg ccgccacatc
    16801 gttaataata tcgatgccgc cgagtgccaa cgctttttcc ataaccaccg tgcggcgcgt
    16861 gtccaaactg acgggaacgc cccatcccgc gacttctacc aatacaggct caacccgcgc
    16921 ccattcttct tcggacgaaa cataatccgc acccggccgc gtcgattcgc cgccgatgtc
    16981 gagaatgtcc gcaccctctt tcaaaagccg ttcggcgtgc gccaaagctg tttgggcatt
    17041 ttgcgaatac gcgccgccgt cggaaaaaga atcgggcgtg agattcacga tacccatgat
    17101 tttcggtttg tccaaaccga tttcaaaccg tcctgcctgc caaacgtgtc gtgccatctg
    17161 aactcctccc aaaataaaaa acagattata tgccgtctga aaccgtcttg tgcgcttcag
    17221 acggcaccgc tattcgggcg gcagacggca tgttgtccga atgtctgctc cgcctttgaa
    17281 tctgccggta tgcctgctat ccgcccgact tttcaaaaca ggttccgacg attccgcacg
    17341 cgcctgccgc ctttgccaag ccgtacagga tttcctgcgg catatcgcgg ttccataatc
    17401 ccgtaatatt cgcaatcacg ggcagatggc tgatttggcg gactttcacg atggattcga
    17461 catccaaacg gtagggatgg cctttggtat ggttcaatac gcccgactcg cccagaatca
    17521 ggtggcggtt gccgcgcgag acgacatatt ctgcggcatt caaccaatct tcggcagaat
    17581 ggtgcttgtc tttacacagc accaagggaa tgttcaggtt tccggcttca ttcaatacgg
    17641 caagatcgga catcagcccg ccgcccaaat acaggatgtc cgcccccgca ttcaaagccg
    17701 cttcgacatg gcggacgttg cggacgcgca ccaatacggg tttccctgca tcatgcgccg
    17761 atgcggtctg ttccgccaac cgtctgcacc gtccccgccc ttcatccgca cttgaagtgt
    17821 cgtataagtt tgccgaagtg aaaaacggat ccagaaacac tgcatccgca ttgcgccata
    17881 ctgacggttc tgcggcgata cggacggttt ccccgccgcc gaaagccacg cctttggcgg
    17941 caacgcggct gtcttccgcc cgattttccc gactgacggt tttccatgta tccaaaatgc
    18001 ggacggcttt ctcgacctcc ggcagcgtct gcacctccct gacgctcaaa accctatcgt
    18061 cgccgattgc gccgatgaca gtacgctcgt cgccgtgaga aatgtgttct cgcagacctc
    18121 tgctgcggat aaaggcgaca acgccggcaa tgtccgcttc ggcggcacgc ctgctcatga
    18181 caataatcat atttcctcct gacacaagaa acggcctacc caaaatagga tttttgcaag
    18241 ccgtgttata ctgtggcgtg ttttacagat tgttcgggct atggatttat tatcggtttt
    18301 ccacaaatac cgtctgaaat atgcggtggc cgtgctgacg atactgcttt tggcggcagt
    18361 cgggctgcac gcttccgtat atcgcacctt cacgcctgaa aacatccgca gccgcctaca
    18421 acaaagcatt gcacacacac accggaaaat ctcgtttgat gcggacattc agcgcaggct
    18481 cctgccccgg ccgaccgtca tcctgaaaaa cctgaccatt accgaacccg gcggcgacca
    18541 gactgccgtt tccgtccaag aaaccaaaat cggattgagc tggaaaaacc tgtggtcgga
    18601 tcagatacag attgaaaaat gggtggtttc gagtgcggaa cttgccctga cgcgcgacgg
    18661 gaaaggtgtt tggaacatcc aagacctgat cgacagccaa aaacgccaag cctcagtcaa
    18721 ccgcattatc gtcgaaaaca gcaccgtccg cctcaatttc ctgcaggaac agcttatcct
    18781 gaaggaaatc aacctcaacc tgcaatcccc cgattcgtcg gggcagccgt ttgaaagttc
    18841 gggcatactg gtttggggaa agctgtccgt cccgtggaaa agcagggggc tgttcctttc
    18901 aaacggcatc ggcccgcccg aaatctcacc gttccatttt gaagcttcca cttcgctgga
    18961 cggacacggc attaccattt ccaccaccgg cagcccttct gtccgcttca acgccggcgg
    19021 agcggatgcc gccggcctcg gcctgcgtgc agacacttcc ttccgcaacc tccacctgac
    19081 cgcccaaatc cccgcgctgg cactcaggaa caacagcatt aaaattgaaa ccgtcaacgg
    19141 cgcatttacc gccggcggcg aatatgcccg atgggacggt tcgttcaaac tcgacaaagc
    19201 caacctgcac tccggcatcg ccaacatcgg caacgccgaa atctccggca gcttcaaaac
    19261 accgcgccac cagaccaact tctccctcaa ttcgccgctc gtatggacgg aaaacaaagg
    19321 gctggacgcg ccgcgcctgt atgtatcgac ccttcaggat accgtcaacc gcctgccgca
    19381 accccgtttc atcagccggc tcgacggttc gctgtccgta ccgaatctgc aaaattggaa
    19441 tgccgaatta aacggcacat tcgaccgcca aaccgttgcc gcgaaattca gatacacaca
    19501 tgaagacgca ccgcatctgg aagccgccgt cgcactgcaa aaattgaacc tgacccccta
    19561 tcttgacgac gtgcggcaac aaaacggcaa aatatttccc gacaccctcg ccaagctgtc
    19621 cggcgacatc gaggcgcacc tgaaaatcgg aaaagtccaa cttcccggcc tgcaactgga
    19681 cgatatggaa acctacctcc acgccgacaa aggccatatc gcgctcagcc gtttcaagtc
    19741 agggctttac ggcggccata ccgaaggcgg catcagcatc gccaacaccc gtcccgccac
    19801 ttaccgcctg caacagaatg caagcaacat ccaaatccaa ccgctgctgc aagacctgtt
    19861 cggcttccac agcttcagcg gcaacggcga cgcggtcatc gacctgaccg cgggcggcga
    19921 aacccgaaaa gagcttatcc gctcgcttca gggcagcctg tcgctaaata tttccaacgg
    19981 tgcatggcac ggtatcgaca tggacaatat cctgaaaaac ggcatttcgg gcaaaactgc
    20041 cgacaatgcc gcacccagca cacccttcca ccgattcacg ctcaacagcg aaatttcaga
    20101 cggcatcagc cgccacatcg ataccgaact cttctccgac agcctctatg ttaccagcaa
    20161 cggctatacc aatctggata cgcaggaatt gtctgaagat gtccttatcc gcaacgccgt
    20221 ccatccgaaa aacaaaccga ttcccctgaa aatcaccggc acggtggaca aaccgtccat
    20281 taccgtcgat tacggcaggc tgaccggcgg catcaattcg cgcaaagaga aacagaaaat
    20341 cctcgaagac accctgctgg aacaatggca gtggctcaaa cctaaagaac cgtaaacatc
    20401 ctgcgtacaa aaatgccgtc tgaaacaccc ccgcgcttca gacggcagac cgtaaaacct
    20461 acaaccccaa ttcctcccaa atcccatcaa tcttagccgt aaccgcaggg tcttttttga
    20521 tgacgcgtcc ccattcgcgg tcggtttctc ccggccattt gttggtcgca tccaaaccca
    20581 ttttgccgcc gagtccgctg acggggctgg cgaagtcgag ataatcgatg ggcgtgtttt
    20641 ctaccaaaac agtgtcgcgc acggggtcca tgcgcgtggt gaccgcccag atgacttctt
    20701 tccagtcgcg cacgttcaca tcgtcatcca ccacgatgat gaatttggta tacataaact
    20761 ggcgcaggaa cgaccagcag cccatcatca cgcgcttggc gtgtccggcg tactgttttt
    20821 tcatgctcac caccgccatg cggtaggagc agccttcggg cggcaggtag aaatcggtga
    20881 tttcggggaa ctgcttttgc aaaagcggta cgaacacttc gttcaacgcc acgcccaaaa
    20941 cggcgggttc atcgggcggt ttgcccgtgt aggtcgaatg gtaaatcggg ttttcgcgca
    21001 tggtgatgcg ttcgaccgta aacacaggga aataatcctg ctcgttgtaa tagccggtgt
    21061 ggtcgccgta cgggccttcc aacgcggttt cgttcggatg gatgacgcct tccaacacga
    21121 tttctgcgcg ggcaggcact tgcaaatcgt tgccgataca tttcaccagc tccgtccgcg
    21181 aaccgcgcag cagtccggca aactggtatt cgctcaaggt atcgggaaca ggcgttaccg
    21241 cgcccaaaat ggtggcgggg tcgcagccga gtacgacggc gacgggatac ggcgtatcgg
    21301 gattgagttt gcggaactcc tgataatcca acgcgccgcc gcgatgcgac agccaacgca
    21361 taatcagctt gtttttgccg atgagttgtt ggcggtaaat gccgagattt tggcgttttt
    21421 tgtgcggccc gcgcgtgacg gtcaagcccc acgttaccag cggcgcaacg tcttccggcc
    21481 agcaatgctg aatcggaagt tgatacaaat caacgtcttc gccttcccac acgatttcct
    21541 gacacggcgc gtttttcacc acgttcggcg ccatgctcca aatgtctttc agcagcggca
    21601 gtttggaaaa cgcatctttg atgcctttgg gcggttcggg ttctttcaaa tacgccagcg
    21661 tctgcccaat ttcacgcagc ttggacacgc tgtccgcgcc catgcccatc gccacacgtt
    21721 cgggcgtgcc gaacaggttt gccaacacgg gataaccgta gcgcgtaccg tcgggcttaa
    21781 tcgggttttc aaacagcaac gccggccctt cggcacgcag cacgcggtcg gcgatttcgg
    21841 tcatttccaa atacggggaa atggggtgtg cgacgcgctt gagtttgccc tgctgctcga
    21901 gcatggcgat gaagtcgcgc aggtctttgt atttcatatt catccttttt gtccttttat
    21961 cctgaacaat ccgattcgga taccgcccct atccttgcct gtgcctcggc atattctatg
    22021 ccgtgataaa agtcgcgtac cagcggatgt tcgccgcctt gatggagttg caacaaagga
    22081 cgttgaccat cgggttgggt aacgacattg cagtgcagac cgaaggtgtc ggtttcataa
    22141 gggggcagct ggttgcagat catgccgaaa taaacagcgt tttcaaggtt gtcgtaaaag
    22201 cggctttgat agtcgttaaa actcttttcg ctggcggata cccacacgcc atattccagc
    22261 gtttcctcat gcccgataat cggaatcggc agcaccgcgc ggataaagcg gtcggtttgg
    22321 tcgggatagc ggatgatgca gaaatcagaa tcgcattctg cttgataagc aatgcgttct
    22381 tcttcactga gttgattata gggatcgggt gcggtaaagc cgattgcggg catttcttcg
    22441 tggttttcgc cgcaggaagt gcaagtgtac ataaggtttc agactttcaa aacgagtttg
    22501 cggtaaagcc attcgccggc aaacagcatc cccatcaata cataggcaat cacgccggta
    22561 taaaccgccc accaatcata tcgccccaac cgtgccaaca aagcggcaag cgtcccgttg
    22621 attataaaaa atacgcacca aacctgcgtt acccggcggg tatagcgcac ggctttttca
    22681 ggcaggtcgg gctgttgcag ccgcgcaagt ttttcaatca ccgtctgccc ggcaaacagg
    22741 ctgccgccga acaccgccaa catcatcaga ttgacgagga cgggatacca atacatcgaa
    22801 tcatgccgcc cgaacaccaa tactgcggca aaaaacagtg taataaacaa agccgcataa
    22861 cgctgttggg gcagtttggc agtcagggcg cgcagcagcc acaacccgca catcgccgcc
    22921 gccagccaaa caaaccagcc cgcctccctg ccgtatatag tggattaaca aaaaccagta
    22981 cagcgttgcc tcgccttgcc gtactgtctg cggcttcgtc gccttgtcct gatttttgtt
    23041 aatccactat accacaaagc gggataggca atgcttaata cggtcagaaa aatatgtccg
    23101 aaaaaaccgg gtttcatttt gaatccgcac aaatgttttc agacggcatc cgataaaaac
    23161 atgccgtctg aaaaataatt agaaataccc gattagcccg cctgaatctt caataccgcc
    23221 tgtaccacgt cgttgacggt gcggacattg cggaaatctt cggcctgcag cttgcggccg
    23281 gtttcgcgct tgatgcggtc aatcaggtcg atggcatcga tgctgtcgat ttccaaatct
    23341 tcgtaaagat tggtatcagg cgtaatccgt tccggttcga tttcaaacaa ctcggtcagg
    23401 gtatcgcgca acaaccggta gatttcttgt tcggtcatat gtttcatcct tatgcttggc
    23461 ggcttttgac aaaggcggcg agtgttttga cattggcaaa atgttcgcgc aagttctcct
    23521 gctcgccgtc caatcggaaa ccgaaatgtt tttgcaccgc caagcccaat tccagcgcat
    23581 cgacggaatc cagtcccagc cctccgtcgc cgaacagcgc gtcttcgctg ccgatgtcgg
    23641 cggcggttat atcctccaaa gccaaactgt cgataatcag ttgtttgatt tggttttcca
    23701 agtcgttcat atggttttcc ttgtaaaata gtcctgcaga tattcattca gccgccgcgc
    23761 cgcaatcggc aacggtttct cggcgagcca gtcttggggg aggatgtcgt ctccgacggt
    23821 aatttcatac cgtatccttt tcggggggat gcggtaccac ggctgcccct ttttaaaatt
    23881 gggcggattc attttgatac atacgggcgt aatcacttcg gcataccgca gtcccaaaga
    23941 aaccgcgccc cggtgcattt ttacccgtcc gtcccacccc gtcctcgttc cttcggggaa
    24001 caccagcagg ctctgcccgc tgtcaaaaac cgcctttacc gtttccagca ttgcttccga
    24061 ctcttcgttc ggaatatagc ccgcaccttt aatctggctg ctcattgccg gattgtgctg
    24121 caaatctttt ttcacgatac agttcatttc gggcacatgg ccgacaagca gcaccacatc
    24181 cagcaaagac ggatggtttg ccaaaatcaa ctgtcccggg cggttgagtt tttcaacacc
    24241 cttgaacgat acctccaaca cgcccgacca tttcagataa gcaacgaaca aacgccaaga
    24301 tgttccgata atccggcgcg ccgccaattg gcgggcgaca gaccctgaag tgccgttcaa
    24361 agtataaggc aacaaaacca atttcatcat aatgccgccg acaccgaaaa tcacaaatcc
    24421 taaccaagtc gcaaaaaaac ggcggcaata atccaattta tccattccgc tgccacagcc
    24481 attcacgatt gcgatatacc cggcggcatt ctcgactgcc gttcagcaga aagcgcaccc
    24541 attccaaacc gctccaatat gcctcgggca gcataccggc ttcagacggc atatcgtccg
    24601 aagcagacaa agtcaggctg taacgcgtcc ctttggtcag aaccatcgcc aaagcataag
    24661 caaacggcgc gcgagtcgcc gatacggcat atccttccgg cagcggatcg tccgccgcca
    24721 aaaccaaaac cgacccgcat ccctcttcca acagtgatgc cgcttccgcc aatgccgttt
    24781 ccacaccgtc ggcacacacg gccaatgccg tctgctcgct catatcctga cgcaatatcg
    24841 accattgccc cgccgttgca ttgtgcaccg acaaaccgaa cgaagtcggc gacacggtat
    24901 gcgatttcaa cagttccaac cacaaatcga aactgcgtgc catttccccg tcgtgcgagg
    24961 cataaactac cggactgccg ggatgggcgg aggcaatgtc ccaagccgcg tcgcatacca
    25021 aacgcgccgc cttactcaaa cggcggcgct gcatagcggg caggaacggc aattccggcc
    25081 tgacatcggg caaaccgtcg gcaaaatccg gacattccgc ccattttgcc cactgggcca
    25141 tatcgcgcat tttgctgccc gaaacccgcc aggcggcgat gtcgaagtgg aaccggcaaa
    25201 taatcgacgg catagtttct ttcaaaaatt tacactgtgc cgcattctaa ccaaagccta
    25261 tccccctgac aatgccgaaa ttcaaacgca tttctgcccc ctttctccga caacgccgcc
    25321 cctcggaaaa ccgccagaat tagcctgaat ttacatttat cattataatg cccgtatttg
    25381 ccagcctgcc gccgcaatat atggacacac tgccagaatg cccgattacc aacaccgcct
    25441 ccctgctgcc gcacagcggg cgtatggttc tgatagaccg cattacccga tacggcgatg
    25501 attttgtcga agcaggggca caggtaagcc ccaatcacat ccttttactt gacgacaaac
    25561 tgccctacac ggcatttatc gaactgatgg cacaggctgt cggcgcgtat gccggtatcc
    25621 aagcccgaaa aaacgcacgg tcggtccggc tcggcttcct gctcggcacg cgcaaacttg
    25681 aaatcttcgc ccaatccgtc ccaatcggca cgcatctgct ggcaacggcg catatgtcta
    25741 ttcaggatgc cgggggtatg ggcgtgtttg actgcgaact gcgttggaca gacgcgccgg
    25801 aaacttcgtc cgaaacactc ccttcagacg gcattttggc gcgcgcctca ctcaacgtgt
    25861 acagccccga acaccctgcc ggaacaaccg atgccgtctg aacaggcaca cacatacgga
    25921 gaacaacgat gaccgaaact gtcctgatta ccggctccaa caggggcata ggcaaagccg
    25981 tcgcattcgg tttggcggaa gacggctttg atatcgctgt ccactgccgc agtcgccgcg
    26041 acgaagccga agccgtggcg gaagaaatcc gcgctttggg cagaaatgcg cgcgtgttgc
    26101 agtttgacgt gtccgaccgc gaagcctgcc gcgagattct gaccgccgac atcgaagcaa
    26161 acggcgcgta ttacggcgtg gtgttgaacg ccggactgac gcgcgacaat accttccccg
    26221 cgttttcaga tgacgattgg gatgtggtgc tgcggactaa tttggacggt ttttacaatg
    26281 tattgcatcc gctggttatg ccgatgatac gccgccgcaa agccggacgg attgtgtgta
    26341 tggcatcagt gtccggcctg acgggcaacc gcgggcaggt caattacagc gcgtcaaaag
    26401 caggcattat cggcgcggca aaagccttgg cggtcgaact ggcgaaacgc aaaatcaccg
    26461 tcaactgtgt cgcgccgggt ctcatcgata ccgatattat cgatgagaac gtacctgtcg
    26521 aagaaatctt aaaggctgtc cccgcagcgc ttatggggct gccggaagaa gtggcgcacg
    26581 cggtgcgttt cctgatggat gaaaaagcgg cgtacatcac gcgccaggtg attgcggtga
    26641 acggaggttt gtgttgaata ccagaagggt cgcagtaaca ggcataggcg gcattaccgc
    26701 cttcggccgg gattggcaaa gcatacaggc agcattcaaa gccgaaaaaa acgccgtcaa
    26761 atatatggat tggcacgaac gtttccccga attggaagcg caactgggtg cgccgattga
    26821 ggattacgcg ccgccgaaac attggacgcg caagcagctc agaagtatgg ggcgtgtgtc
    26881 gtatttgtgc gtcgatgcgg cggagcaggc tttgacggat gccggtttgc tcggggacga
    26941 aagcattacc gacggacgga tgggcgttgc ctgcggctct tccagcggca gcaccaaaga
    27001 catcggcgat gtaggcgaat tgttgctgac cggcatgtcg cgcaacttca gtgccaacac
    27061 ctatgtgcgt atgatgccgt acaccgccgc cgccaatatc ggcatctttt tcggactgaa
    27121 aggacgcatc atcccgacat cgagcgcgtg ttcgtccggc agccaaggca taggttatgc
    27181 ctacgaagcc atcaaatacg gtctgaccga tatgatgctg gcgggcggag gcgaagaatt
    27241 tttcccgtcc gaagtgtatg ttttcgactc gctttatgcc gccagccgcc gcaacggcga
    27301 accggaaaaa accccgcgcc catacgacgc gaaccgcgac gggctggtca tcggcgaagg
    27361 cgcggggatt ttcgtgctgg aagaattgga acacgccaaa cggcgcggtg cgataattta
    27421 cgccgaactc gtcggctacg gagccaacag cgatgcctac catatttcca cgccccgccc
    27481 cgacgcgcaa ggcgcaatcc ttgcctttca gacggcattg caacacgcag gtcttgcgcc
    27541 cgaagacatc ggctggatta atctgcacgg caccgggacg caccacaacg acagtatgga
    27601 aagccgtgcc gttgcagcgg ttttcggcaa caatacgccc tgcacgtcca ccaagccgca
    27661 aaccggacac acgctgggtg cggcgggcgc aatcgaagcc gcgttcgcgt ggggctttgc
    27721 cgaccggcaa agcaatcccg aaggaaaact tccgccccag ctttgggacg ggcagaacga
    27781 ccccgacctg cccgccatta acctgaccgg cagcggcagc ttctagacaa cggaaaaaca
    27841 cattgccgcc agctcgtcgt ttgccttcgg aggaagcaac tgcgtcttaa tcatcggatg
    27901 aaataagttt gtcaatccca ccactatgct atacaatacg cgcctactct tgacgggtct
    27961 gtagctcagg ggttagagca ggggactcat aatcccttgg tcgtgggttc gaaccccacc
    28021 ggacccacca attcccaagc ccggacgtat gtttgggctt ttttgccgcc ctgtgaaacc
    28081 aaaatgcttt gagaaacctt gataatgaaa aaagtcagcg tattgattgt tgccaaaaac
    28141 gaagcaaacc acattcagga atgtattgaa agttgccgtt tcgataaaga agtcatcgtt
    28201 atcgacgact acagcaccga caatactgcc gaaattgccg agggtttggg cgcaaaagtc
    28261 ttcagacggc atttgaatgg ggatttcgga gcgcaaaaaa catttgccat cgaacaggca
    28321 ggcggagaat gggttttcct gattgatgca gacgaacgct gcacgccgga actatctgat
    28381 gaaatctcaa aaattgtcca aaccggcgat tatgccgcct attttgtcga acgccgcaac
    28441 cttttcccca accatcccgc cacacacggc gcgatgcgtc ccgacagcgt atgccgtctg
    28501 atgccgaaaa aagacagttc ggtgcaaggc aaagtacacg aaaccgtaca aaccccctac
    28561 cccaaacgcc gtctgaagca ttttatgtac cattacacgt acgacaactg ggaacaatat
    28621 ttcaacaagt tcaacaaata tacttccatt tcagccaaaa aataccgaga gcagggaaag
    28681 cccgtgcgtt tcgttaggga cattatcctc cgcccgattt gggggttttt caaaatttat
    28741 atcctgaaca aagggtttct tgatggaaaa atgggttgga ttatgtccgt caaccacagc
    28801 tattacacga tgattaaata tgtcaaacta tattatctgt acaaatccgg cggaaaattt
    28861 taaatggaaa aagaattcag gatattaaat atcgtatcgg ccaagatttg gggtggaggc
    28921 gaacaatatg tctatgatgt ttcaaaagca ttggggcttc gggactgcac aatgtttacc
    28981 gccgtcaata aaaataatga attgatgcac aggcgatttt ccgaagtttc ttccgttttc
    29041 acaacgcgcc ttcacacgct caacgggctg ttttcgctct acgcacttac ccgctttatc
    29101 cggaaaaacc gcatttccca cctgatgata cacaccggca aaattgccgc cttatccata
    29161 cttttgaaaa aactgaccgg ggtgcgcctg atatttgtca aacataatgt cgtcgccaac
    29221 aaaaccgatt tttaccaccg cctgatacag aaaaacacag accgctttat ttgcgtttcc
    29281 cgtctggttt acgatgtgca aaccgccgac aatcccttta aagaaaaata ccggattgtt
    29341 cataacggta tcgataccgg ccgtttccct ccctctcaag aaaaacccga cagccgtttt
    29401 tttaccgtcg cctacgccgg caggatcagt ccagaaaaag gattggaaaa cctgattgaa
    29461 gcctgtgtga tactgcatcg gaaatatcct caaatcaggc tcaaattggc aggggacgga
    29521 catccggatt atatgtgccg cctgaagcgg gacgtatctg cttcaggagc agaaccattt
    29581 gtttcttttg aagggtttac cgaaaaactt gcttcgtttt accgccaaag cgatgtcgtg
    29641 gttttgccca gcctcgtccc ggaggcattc ggtttgtcat tatgcgaggc gatgtactgc
    29701 cgaacggcgg tgatttccaa tactttgggg gcgcaaaagg aaattgtcga acatcatcaa
    29761 tcggggattc tgctggacag gctgacacct gaatctttgg cggacgaaat cgaacgcctc
    29821 gtcttgaacc ctgaaacgaa aaacgcactg gcaacggcag ctcatcaatg cgtcgccgcc
    29881 cgttttacca tcaaccatac cgccgacaaa ttattggatg caatataaac tgctttcaga
    29941 cggcatatgc cgtctgaaag cctttgatgc aacaaaccac taaattatat tcgttcattg
    30001 gaaagaaaca ccccgaattc atccttcaaa ataagaaaat cccaatatcc cccgatatta
    30061 cgcagcctat tggcaaagtt ttgcagcgtc ttccccggct tgtgctgccg cgtcaagtgc
    30121 tttgttacaa tgtatagtag a
//
LOCUS       DAWIVB010000006        27738 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWIVB010000006 DAWIVB010000000
VERSION     DAWIVB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.006"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            182..538
                     /locus_tag="WIX60_000181"
     CDS             182..538
                     /locus_tag="WIX60_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ5304512.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            529..1011
                     /locus_tag="WIX60_000182"
     CDS             529..1011
                     /locus_tag="WIX60_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ5304513.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1024..1617
                     /locus_tag="WIX60_000183"
     CDS             1024..1617
                     /locus_tag="WIX60_000183"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ5304514.1"
                     /translation="MASIQDLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1607..2863
                     /gene="nuoD"
                     /locus_tag="WIX60_000184"
     CDS             1607..2863
                     /gene="nuoD"
                     /locus_tag="WIX60_000184"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ5304515.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLVGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2863..3336
                     /gene="nuoE"
                     /locus_tag="WIX60_000185"
     CDS             2863..3336
                     /gene="nuoE"
                     /locus_tag="WIX60_000185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ5304516.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3726..5027
                     /gene="nuoF"
                     /locus_tag="WIX60_000186"
     CDS             3726..5027
                     /gene="nuoF"
                     /locus_tag="WIX60_000186"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ5304517.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5027..5464
                     /locus_tag="WIX60_000187"
     CDS             5027..5464
                     /locus_tag="WIX60_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304518.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRGNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            5708..5989
                     /locus_tag="WIX60_000188"
     CDS             5708..5989
                     /locus_tag="WIX60_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ5304519.1"
                     /translation="MRNHPLTRQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYQFFENMPEAVAKEKTMKKWRREWKIKLIEEQNTE"
     gene            6301..8562
                     /locus_tag="WIX60_000189"
     CDS             6301..8562
                     /locus_tag="WIX60_000189"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ5304520.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGKQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAVHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8565..9641
                     /gene="nuoH"
                     /locus_tag="WIX60_000190"
     CDS             8565..9641
                     /gene="nuoH"
                     /locus_tag="WIX60_000190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ5304521.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            9722..10201
                     /gene="nuoI"
                     /locus_tag="WIX60_000191"
     CDS             9722..10201
                     /gene="nuoI"
                     /locus_tag="WIX60_000191"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ5304522.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            10232..10939
                     /locus_tag="WIX60_000192"
     CDS             10232..10939
                     /locus_tag="WIX60_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304523.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            10975..11646
                     /locus_tag="WIX60_000193"
     CDS             10975..11646
                     /locus_tag="WIX60_000193"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ5304524.1"
                     /translation="MTFQLILFYIFAVIILYGALKTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDDLKPK
                     EEGKA"
     gene            11643..11948
                     /gene="nuoK"
                     /locus_tag="WIX60_000194"
     CDS             11643..11948
                     /gene="nuoK"
                     /locus_tag="WIX60_000194"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ5304525.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            11982..12557
                     /locus_tag="WIX60_000195"
     CDS             11982..12557
                     /locus_tag="WIX60_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ5304526.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            12681..13022
                     /locus_tag="WIX60_000196"
     CDS             12681..13022
                     /locus_tag="WIX60_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304527.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            13071..15095
                     /gene="nuoL"
                     /locus_tag="WIX60_000197"
     CDS             13071..15095
                     /gene="nuoL"
                     /locus_tag="WIX60_000197"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ5304528.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPVTWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAVPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTIHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            15191..16687
                     /locus_tag="WIX60_000198"
     CDS             15191..16687
                     /locus_tag="WIX60_000198"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ5304529.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGSDSRAPFARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLIMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIEQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     QDINCREFAILAILAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            16697..18139
                     /gene="nuoN"
                     /locus_tag="WIX60_000199"
     CDS             16697..18139
                     /gene="nuoN"
                     /locus_tag="WIX60_000199"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ5304530.1"
                     /translation="MNWSDLNLMPAMPEIVLLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFTFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMFAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVIMSLIGAFYYLRVVKVIYFDVPDHDQPVGSNYAAKFV
                     LTVNAFLLLLWGIMPQTVIDWCAKALENTL"
     gene            18251..18550
                     /locus_tag="WIX60_000200"
     CDS             18251..18550
                     /locus_tag="WIX60_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ5304531.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAVLAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(18659..19555)
                     /locus_tag="WIX60_000201"
     CDS             complement(18659..19555)
                     /locus_tag="WIX60_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ5304532.1"
                     /translation="MNPTNDLKTWQQRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(19539..19763)
                     /locus_tag="WIX60_000202"
     CDS             complement(19539..19763)
                     /locus_tag="WIX60_000202"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ5304533.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDTDGLKELNLESDE"
     gene            complement(20010..20933)
                     /gene="rsgA"
                     /locus_tag="WIX60_000203"
     CDS             complement(20010..20933)
                     /gene="rsgA"
                     /locus_tag="WIX60_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ5304534.1"
                     /translation="MPSEHPFSDGIPTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(20938..22794)
                     /locus_tag="WIX60_000204"
     CDS             complement(20938..22794)
                     /locus_tag="WIX60_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5304535.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPRALPLNVPQGAIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(22820..22957)
                     /locus_tag="WIX60_000205"
     CDS             complement(22820..22957)
                     /locus_tag="WIX60_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304536.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(22957..23541)
                     /gene="ruvA"
                     /locus_tag="WIX60_000206"
     CDS             complement(22957..23541)
                     /gene="ruvA"
                     /locus_tag="WIX60_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ5304537.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(23623..23949)
                     /locus_tag="WIX60_000207"
     CDS             complement(23623..23949)
                     /locus_tag="WIX60_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ5304538.1"
                     /translation="MNIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(24011..24730)
                     /locus_tag="WIX60_000208"
     CDS             complement(24011..24730)
                     /locus_tag="WIX60_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ5304539.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFTSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(24727..24900)
                     /locus_tag="WIX60_000209"
     CDS             complement(24727..24900)
                     /locus_tag="WIX60_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ5304540.1"
                     /translation="MKRGGNTVTQAAFERRFMPSENLSGHIPNRRPHCTAVRNVSGGH
                     DAHSSSTQERNTF"
     gene            complement(24860..25324)
                     /gene="trmL"
                     /locus_tag="WIX60_000210"
     CDS             complement(24860..25324)
                     /gene="trmL"
                     /locus_tag="WIX60_000210"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ5304541.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(25389..25886)
                     /locus_tag="WIX60_000211"
     CDS             complement(25389..25886)
                     /locus_tag="WIX60_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ5304542.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            25942..26862
                     /gene="bioH"
                     /locus_tag="WIX60_000212"
     CDS             25942..26862
                     /gene="bioH"
                     /locus_tag="WIX60_000212"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ5304543.1"
                     /translation="MCLKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQRERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFVRPFDIAAAADGIAAQIDAPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TADEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPQALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRRLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGGLR"
     gene            26859..27647
                     /locus_tag="WIX60_000213"
     CDS             26859..27647
                     /locus_tag="WIX60_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ5304544.1"
                     /translation="MNHQDARWQVHRHLAEHTDQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         6126 a         6897 c         7722 g         6993 t
ORIGIN      
        1 cgaattacgg tgtaaaaatt tatatgacct tataaaatca aataagaatc gttatcataa
       61 catgattgta tttattgggt ttttttgggc gttttgccga tatttacctt ttaatggttt
      121 ttgaaattcg ctaaaatacg aaattattgt agaaattttg ttaacggatt tgggtgtaac
      181 catgttgtcc gcttactttc ccgtctttgt ctttatcctc atcggcctcg cggccggcgt
      241 gctgtttatc ctgctcggca cgattttagg cccgaaacgc cactatgccg aaaaagacgc
      301 gccttacgaa tgcggttttg aagcttttga aaacgccagg atgaagttcg acgtgcgcta
      361 ttacctcgtc gccatcctct tcatcctgtt tgatttggag gtcgcgttta tgctgccgtg
      421 ggcagtcgtg ttcaaagatt tgggcgcgta cggcttctgg tctatgctgg tgtttatcgt
      481 tgttctgacg gtaggctttg tttacgaatg gaaaaaaggt gcgctggaat gggaatagaa
      541 ggcgttttga aaaaaggttt catcaccacc agcgcggata cggtgctgaa ctatatgcgt
      601 accggttcgt tgtggccggt tactttcggc ttggcctgct gcgccgtgga aatgatgcac
      661 gcgggtatgg cgcgttacga ccttgaccgt ttcggtatta ttttccgtcc gtccccccgt
      721 caggccgacc tgatgattgt ggcgggtacg ctcaccaata aaatggcgcc cgccctgcgc
      781 cgagtgtacg accagctcgc cgagccgcgc tgggtattgt ctatgggctc atgtgccaac
      841 ggcggcggct attatcacta ttcttattcc gttgtgcgcg gtgccgaccg cgtcgtgccg
      901 gtagatgttt atgtgccggg ttgtccgccg actgcggaag ccctgattta cggcctgatt
      961 cagctccaac aaaaaatcaa gcgcacttcc accattgcgc gtgacgagta aggagaggac
     1021 gatatggcaa gcattcaaga cttatacgaa accgtcagcc gcgttttggg caatcaggca
     1081 ggcaaagtca tttccgcttt gggcgagatt accgtcgagt gtctgcccga gcactatatt
     1141 tcagtcatga ccgcattgcg tgaccatgaa gagttgcatt tcgagcttct ggttgacttg
     1201 tgcggtgtcg attacagcac ttacaaaaac gaagcatggc agggcaaacg ctttgccgtc
     1261 gtcagtcagt tgctttccgt taaaaacaat caacgcatcc gcgtgcgcgt ctgggtttca
     1321 gacgacgact tccccgtagt cgaatctgta gtcgatattt acaacagcgc ggattggtac
     1381 gaacgcgaag ccttcgatat gtacggcatc atgttcaaca accatccgga cttgcgccgc
     1441 atcctgaccg attacggctt cgtcggacat ccgttccgca aagacttccc gatttccggc
     1501 tatgtggaaa tgcgttacga cgaagagcaa aaacgcgtga tttaccaacc tgttaccatt
     1561 gagccgcgcg agatcacgcc gcgtatcgtc cgtgaggaga actacggtgg ccaataaatt
     1621 aagaaactac accatcaact tcggcccgca acaccctgcg gcgcacggcg tattgcgtat
     1681 gattttggag ctggacggcg aacaaatcgt ccgtgccgac ccgcatatcg gcctcttgca
     1741 ccgaggtacc gaaaaactgg cggaaaccaa aacctatctg caagccctgc cctatatgga
     1801 ccgcttggac tatgtttcca tgatggtcaa tgagcaggcg tattgtttgg cagtagaaaa
     1861 acttgtcggt atcgatgtgc ccatccgcgc ccaatacatc cgcgtgatgt ttgccgaagt
     1921 aacgcgcatc ctcaatcact tgatgggcat cggttcgcat gccttcgaca tcggcgcgat
     1981 gaccgccatt ctttacgcct tccgcgaccg cgaagagctg atggacttgt acgaagccgt
     2041 gtccggcgcg cgtatgcacg ccgcctactt ccgtcccggc ggcgtttacc gcgacctgcc
     2101 cgactttatg cccaaatacg agggcagcaa attccgcaat gccaaagtat tgaagcagct
     2161 caacgaatcc cgcgaaggca ccatgctcga ctttatcgat gccttctgcg aacgcttccc
     2221 caaaaatatc gacacactcg aaaccctcct gaccgacaac cgtatttgga aacagcgtac
     2281 cgtcggcatc ggcgtcgtct cccccgaacg tgccatgcaa aaaggcttta ccggcgtgat
     2341 gttgcgcggt tcgggcgtgg aatgggacgt gcgtaagaca cagccttacg aagtgtacga
     2401 caaaatggat ttcgacatcc ctgtcggcgt gaacggcgac tgctacgacc gctacctctg
     2461 ccgtatggaa gaaatgcgtc aatccgtacg catcatcaaa caatgttccg agtggttgcg
     2521 tgtcaatccg ggtccggtca ttaccacaaa ccacaaattc gctccgccca aacgtaccga
     2581 aatgaaaaca ggtatggaag acctgattca ccatttcaaa ctctttaccg agggtatgca
     2641 cgttcccgag ggcgagacct acaccgctgt cgaacatccg aaaggcgagt tcggcgttta
     2701 catcatttca gacggcgcaa acaaacccta ccgcctgaaa atccgcgcac ccggcttcgc
     2761 ccatctgcaa ggcatggacg aaatggcaaa aggccacatg ctcgccgacg tcgttgccat
     2821 catcggtacg caggacatcg tattcgggga ggttgaccga taatgttatc cgcagaatct
     2881 ttaaaacaaa tcgacatcga gttggcaaaa tatcctgccg accaacgccg ctccgcgatt
     2941 atgggcgcat tgcgtattgc ccaaaccgaa aaaggctggc ttgctcccga gaccatcgct
     3001 tttgtcgccg actacatcgg catcacgcct gcacaagcct acgaagtcgc cactttctac
     3061 aatatgtacg accttgagcc tgtcggcaaa tacaaactga ccgtttgtac caacctgccc
     3121 tgcgccctgc gcggcggtat ggctaccggc gaatacctca aacaaaaact cggtatcggc
     3181 tacggcgaaa ctacccctga cggcaagttt acccttgtcg aaggcgaatg catgggcgca
     3241 tgcggcgacg ctcccgttat gctggtcaac aaccacagca tgtgcagctt tatgaccgaa
     3301 gaagcgattg agaagaaact ggcggagttg gagtaggtcg tctgaaacga cgatttaaac
     3361 gtaggtcgga tacttgtagc cgacagagtg ggtaaaaagg caaaatgtcg gatttaagaa
     3421 tccgccctac tgaaataccg aaatgccgtc attcccgcgc aggcgggaat ccaccggtaa
     3481 gattcggttt ctgaatttaa taagacattg cttaccattg aggatggatt cccgcctgcg
     3541 cgggaatgac gacagacaag caagtggtcg agatccaaca aaaacgatta aaggtcgtct
     3601 gaaaatatcg atttgataaa ctagatttta tttcagacga cgttacaagc cggtacacaa
     3661 agacatctta aggtcgtctg aaacagcggc cgcaaccgat acgaaaacaa acaggcacac
     3721 caaaaatggc tatttaccaa tcaggcgtga tttttgacca agtggatacc gccaatcccg
     3781 attgctggac attggacgaa tacgtcaaac gcggcggcta taccgccctg cgtaaaattc
     3841 tgtccgaaaa catctcgcaa accgatgtga ttgacgaagt caaaacctcc ggtttgcgcg
     3901 ggcgcggcgg tgcgggcttc ccgaccggtt tgaaatggag ctttatgccc cgttctttcc
     3961 cgggcgaaaa atatgtggtt tgcaacaccg acgaaggcga accaggtacg tttaaagacc
     4021 gcgacatcat catgttcaat ccgcatgccc tgatcgaagg catgattatc gccggttacg
     4081 cgatgggcgc gaaagccggt tacaactata tccacggcga aatttttgaa ggctaccaac
     4141 gctttgaggc cgctttggag caggcgcgtg ccgcaggctt tttgggtaaa aatattttgg
     4201 gttcggattt tgaatttgaa ctcttcgccc accacggcta cggcgcatat atttgcggcg
     4261 aggaaaccgc attgctcgaa tcgctggaag gcaaaaaagg ccagccgcgc tttaagccgc
     4321 cattccctgc ttcgttcggc ctgtacggca aaccgactac catcaacaat actgaaacgt
     4381 tctcctccgt tccattcatt atccgtgacg gtggacaggc atttgccgat aaaggtattc
     4441 cgaatgcagg cggtaccaaa ttattctgta tttccggcca tgtcgagcgt ccgggcaact
     4501 atgaagtgcc attgggtacg ccgtttgccg aagtcttgaa aatggcgggc ggtatgcgcg
     4561 gcggtaaaaa actcaaagcc gtcattcccg gcggttcgtc cgcgcccgta ttgcctgccg
     4621 acatcatgat gcagaccaat atggactacg actcgatctc caaagcaggc tccatgctcg
     4681 gttccggcgc gattatcgtc atggacgaag acgtgtgcat ggtcaaagcc cttgagcgtt
     4741 tgagctactt ctactacgac gagtcttgcg gccaatgtac cccctgccga gaaggtacgg
     4801 gctggcttta ccgcatcgtc caccgcatcg tagaaggcaa aggtaaaatg gaagatttgg
     4861 atttgctgga ttccgtcggc aaccaaatgg caggccgcac catctgcgcc ctcgccgatg
     4921 ctgccgtctt ccccgtccgc agctttacca agcatttccg tgatgagttt gtgcattaca
     4981 tcgaacacgg cgggccgatg aaagagcata agtggggagg gtggtaatgg tggaagctaa
     5041 aatttttatt ctatacggtg cagccaacaa aggtaagagt acgacactca atacgctttt
     5101 taatcagatt tgtcggaaat tttctaaatt tctagtcttt tttgaaagac atggaaacgg
     5161 cttagatttt gttgcagtat ttgatcatga aggtcagaga attggttttt attcatctgg
     5221 tgataatgaa tacgaggtta ggggaaattt atacaaactt tattcgcata attgtgattt
     5281 tatttttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat gttatgcaga
     5341 gttattgcat ggcgatgtaa atataattaa ttggtgtgaa aagtttgagc ctacagatga
     5401 agacaatgag cgtgctgtta aagagttatt taagtcattt aaaaatataa taaatgagtt
     5461 atagttttag ttggttttat attggttaaa agcaaaatgc taaaaattta actttgccgt
     5521 cattcccgcg taggcgggaa tccatagtgg aatttacaga acccgatatt tgaaaagcag
     5581 ttgccgaaat tcaaaaaatg gattcccgcc tacgcgggaa tgacggcggg agtaggcaga
     5641 tgttttcaga tgaaaacggt tgtaaatgat attaaaaaag ttgttgttta tattgcagga
     5701 aaaatgaata cgaaaccatc cgcttactag acaacctgcc gtatatattt tggcaaacgg
     5761 taaaaatgga acactctata tcggtgttac catgaatttg ccggaaaggg tttggcagca
     5821 caaaaaccat gtcaatattg atggctttac tgcccgatat gatgtgcatg atttagtttg
     5881 gtatcagttt tttgagaata tgcctgaagc agttgccaaa gaaaaaacga tgaaaaaatg
     5941 gcgacgtgaa tggaagatta aactgattga agaacaaaat actgaatgat tggacttgtc
     6001 gggcgtgttg tttgtttagt tttatttctg gaactttaaa aactgtcgtt attccagccc
     6061 cacctacgcg cagacaggct acggcgggaa tcaccgcaaa agttaagaaa ccaatgtttg
     6121 aaaacagtta ccgaaaaccc aagaatggat tcccgcctac gcgggaatga cggcaaggtg
     6181 gcagtaaacg ttttaaacag tattgattgt caatgaaact caaaaggccg tctgaaaccc
     6241 atttttcaga cgacctccat aaaagattat ttatcaaata cccgtaacta ggaacgaacc
     6301 atgttacaaa tcgaaatcga cggcaaacaa gtatctgtgg agcagggcgc gacggtgatt
     6361 gaagccgcgc acaagctcgg tacttatatt ccgcatttct gttaccacaa aaaactttcc
     6421 atcgccgcca actgccgtat gtgtctggtg aacgtagaaa aagccccaaa acccctgcct
     6481 gcctgtgcca cgccggttac agacggcatg attgtgcgta cgcattcggc aaaagcccga
     6541 gaggcgcagg aaggcgtgat ggagttcctg ctcatcaacc atccgcttga ttgtccgacc
     6601 tgcgaccaag gcggcgaatg ccagttgcag gatttggcgg tgggctacgg caaaaccacc
     6661 agccgctaca ccgaagaaaa acgttccgtc gtcggcaaag atatggggtc cttggtttcc
     6721 gccgaggaaa tgagccgctg tatccactgc acccgctgcg ttcgtttcac tgaagaaatc
     6781 gccggtttgc aggaaattgc gatggtgaat cgcggcgaac actccgaaat catgcccttt
     6841 atcggcaaaa cggtggaaac cgaattgtcg ggcaacgtca ttgatttgtg tcccgtcggc
     6901 gcgctgacca gcaaaccgtt ccgcttcaac gcgcgtactt gggaattgaa ccgccgcaaa
     6961 tccgtttccg cccacgatgc tttgggcagc aacctgattg tgcagaccaa agaccatacc
     7021 gtccgccgcg tgttgccgtt ggaaaacgaa gcgattaacg aatgctggct gtctgaccgc
     7081 gaccgtttcg cctacgaagg cctgtatcac gaaagccgtc tgaaaaaccc gaaaatcaaa
     7141 cagggcggcg agtggatgga cgtggattgg aaaaccgcgt tggaatatgt ccgcagcgcg
     7201 attgaatgta tcgccaaaga cggcaagcaa aaccaagtcg gcgtttgggc gaacccgatg
     7261 aatacggttg aagaactgta tctggcgaag aaactcgccg acggcttggg tgttaaaaac
     7321 tttgcaaccc gtttgcgcca acaagacaaa cgtctttcag acggccttaa aggtgcgcaa
     7381 tggttgggac aaagcattga atctttggct gacaacgatg ccgtattggt agtcggtgcg
     7441 aacttgcgca aagaacagcc gcttctgacc gcccgcctgc gccgcgccgc taaagaccgc
     7501 atggctttga gcgtattggc aagcagtaaa gaagaattgt ttatgccgct tctgtctcaa
     7561 gaagccgtac atcccgacga gtgggcaggt cgtctgaaaa acctgtctgc cgatgcggaa
     7621 cacgccgtta ccgccagcct gaaaaacgct gaaaaagcag cggtgatttt gggcgcggaa
     7681 gtgcaaaacc atcctgatta cgccgccatt tatgccgccg cgcaagagct ggcggacgcg
     7741 accggcgcag tgctgggtat tttgccgcaa gccgccaaca gtgttggtgc ggatgtcttg
     7801 aatgtaaact ccggcgagag cgttgccgaa atggcaaacg cgccgaaaca ggcagtcttg
     7861 ttgctcaacg tcgagccgga aattgatacg gttgacggtg caaaagccgt agccgcgttg
     7921 aaacaggcaa aaagcgtgat ggcgtttacg ccgtttgtca gcgaaacgct gctggacgtg
     7981 tgcgacgtgt tgttgccgat tgcaccgttt accgaaacct caggcagctt catcaatatg
     8041 gaaggccgtc tgcaatcttt ccacggcgtg gtacaaggct tcggcgattc gcgtccgatg
     8101 tggaaagtat tgcgcgtatt gggcaatctg tttgacctga aaggttttga atatcacgat
     8161 accgctgcga ttctgaaaga cgcgctggat gcagaaagcc tgccgtccaa actggacaac
     8221 cgtagcacat ggacagggga gggcgttcag acggcctcag accgccttgt ccgtgtcggc
     8281 ggcgtcggta tttatcacac cgattctatc gtgcgccgtt ccgcaccgtt gcaagaaacc
     8341 agccatgccg ccgtgcctgc tgcgcgtgta aatccaaata cattggcacg cttgggcctg
     8401 caagacggac aaaccgctgt cgccaaacaa aacggcgcaa gcgtatcggt tgccgtcaaa
     8461 gccgatgccg gactgcctga aaacgtggtg catctgccgc tgcataccga aaatgccgcg
     8521 ctgggtgcgt tgatggacac tattgaactg gcgggagctt gattatgcag gaatggttcc
     8581 aaaacctctt tgccgcaacg ctcggtctgg gcgatttggg tattactgta ggcttggtgg
     8641 tatccgtcat cgtcaaaatt gtgattatcc tgattccgct gattctgacc gtcgcctacc
     8701 tgacttattt cgaacgtaaa gtcatcggct tcatgcagct tcgcgtcggt ccgaacgtaa
     8761 ccggcccgtg gggtctgatt cagccgtttg ccgacgtgtt caaactcttg tttaaagagg
     8821 taacccgtcc gaagctgtca aacaaagccc tgttctatat cggcccgatt atgtcgcttg
     8881 ccccgtcttt cgcggcgtgg gcagtgattc cgttcaatga agaatgggtg ctgaccaaca
     8941 tcaatatcgg tcttttgtac atcctgatga ttacctcgct gtcggtttac ggcgtgatca
     9001 tcgcgggctg ggcttccaac tccaaatatt cgttcttggg cgcaatgcgt gcttccgcgc
     9061 aaagcatttc ctacgaaatc gccatgagtg ccgcgctggt gtgcgtcgtg atggtgtcgg
     9121 gcagcatgaa cttctccgac atcgttgccg cgcaagccaa aggtattgcc ggcggttcgg
     9181 tattctcgtg gaactggctg ccgctcttcc ccatcttcat cgtttatctg atttccgccg
     9241 ttgccgaaac caaccgtgcg ccgtttgacg tggcagaggg tgagtctgaa atcgttgccg
     9301 gtcaccacgt cgaatattcc ggcttcgcgt tcgcgctgtt cttccttgcc gaatacattt
     9361 tcatgattct gattgccgcg ctgacatcgt tgatgttcct cggtggctgg ttgtctccgt
     9421 tcccgcaaag ctggggcttt atcggtacgc cttccgcatt ctggatgttc gtgaaaatgg
     9481 cggcggttct gtactggtac ctgtggatcc gtgcaacctt cccgcgctac cgttacgacc
     9541 aaatcatgcg tttgggctgg aaagtgctga ttccgatcgg cttcgcctac atcgtggttt
     9601 tgggcgtgtg gatgatttca ccgctgaatt tgtggaaata agtttcagac ggcatcttga
     9661 ggccgtctga acaaagcgat tttgaatacc taacgaaatc cctgttttga gggaacataa
     9721 tatggctaac ttagtaaaaa cctttctgct tggcgaattg gtaaaaggta tgggcgtaac
     9781 gctcaaaaac tttttcgccc gcaaagacac gatttatttt cccgaagaga aaacgccgca
     9841 atccgtgcgt ttccgcggcc tgcacgcgca acgccgttat ccgaacggcg aagagcgctg
     9901 tattgcgtgt aaattgtgtg aggcagtttg tccggcaatg gcgattaaca tcgaatcgga
     9961 agaacgtgaa gacggtaccc gccgcaccaa gcgttacgac atcgatctga ccaagtgcat
    10021 cttctgcggt ttctgcgaag aggcctgccc gaccgatgcg attgtggaaa cccatatttt
    10081 tgaataccac ggcgagaaaa aaggcgactt gcacatgacc aagccgattc ttttggccat
    10141 tggcgataaa tacgaagctg aaatagccaa acgcaaagcc gctgacgcgc cgtatcgtta
    10201 atgctttggg gcttcttgga aggttttaaa tatggaagga ctgattaatg cattgaaata
    10261 tttagccgaa catgagccaa tagataattt tgaagaaatt agaactagaa atagtccgat
    10321 tgagttgcca agtggattaa gtaattttga acaaaatatt tttttaaaag aaaatttatc
    10381 cccaaaatta caaaatgatg atagcttgaa gacgcattat tggattatcc gtgaatgggg
    10441 tgggattaaa agttttaaac aatctgctga aaatagccag cttattcgtc aatttttatc
    10501 ggaacttaat tcgggaaaat tgagtagtgg tttgttgaaa atttcatcat tatctaaatt
    10561 ggcttctttt atagattgtg agcgattcgc catttatgat tcacgcgcta ttttttcgtt
    10621 gaattggttg ttgtttaaat ttacaaatgc agatttgttt tttcagccac aaggtagaaa
    10681 tagggaacta gaaatccgaa atatgaacgt attgtttcat ttttctgata tcaaaccgaa
    10741 ttatcggaaa ccagacgttt cgtttcatca atattgtggg ttgttacaag atttggcgaa
    10801 acaagtttat ggtaaacaag caaaaccgta tcacatagaa atgttgttat tcaaaattgc
    10861 gacaacgtgg atttgtgcgg atatggatca actgattaag tttgattgtt tgcgtaacca
    10921 ggattttcag actgcttgaa accatatttt tgattaataa agaaagcata gactatgact
    10981 ttccaactga ttttatttta tatttttgca gtgataattc tttatggcgc gctcaaaacc
    11041 gtcaccgcta aaaaccctgt tcacgccgct ttgcatctgg tgctgacctt ctgcgtgagc
    11101 gcgatgcttt ggatgctgat gcaggctgag tttttgggcg tgacgctggt ggtggtttac
    11161 gtcggcgccg tgatggtgtt gttcctgttc gtcgtgatga tgttgaacat cgacattgaa
    11221 gaaatgcgtg ccggtttctg gcggcacgcg cctgttgccg gtgtggtcgg cacattgttg
    11281 gcggttgcgc tgatcctgat tctggtcaac ccgaaaaccg accttgccgc atttggtctg
    11341 atgaaagaca ttcctgccga ttacaacaat atccgcgatt tgggcagccg tatttatacc
    11401 gactatctgt tgccgtttga attggcggcg gtattgctgt tgttgggtat ggtggcggcg
    11461 attgcgctgg ttcaccgtaa aacggttaat ccgaaacgca tggatcctgc cgaccaagtc
    11521 aaagtacgcg ccgaccaggg ccgtatgcgt ctggtgaaaa tggaagcggt caaaccgcaa
    11581 gtcgaatctg ccgaagaaag cgaagtttca gacgacctca agccgaaaga ggagggcaaa
    11641 gcatgattac cttgacgcat tatttggtat tgggtgcgct cctgttcggt atcagcgcaa
    11701 tgggtatctt tatgaaccgc aaaaacgtgc tggtattgct gatgtcgatc gagctgatgc
    11761 ttttggcggt gaacttcaac tttatcgcct tctcgcaaca tttgggcgat actgccggac
    11821 aaattttcgt attcttcgta ttgaccgttg ccgctgccga atctgccatc ggtttggcga
    11881 ttatggtgct ggtgtaccgc aaccgacaaa caatcaacgt tgccgatttg gacgagttga
    11941 aagggtaaag gtaggttggg tcgagacctg acaagacacc gatgccgtct gaaaacccga
    12001 taggaaaaac gatgaaatcc atagacgaac aaagcctgca taatgcccgc cgcctgtttg
    12061 aaagcggcga catcgaccgt atcgaagtcg gtaccaccgc gggcctgcaa cagattcacc
    12121 gttacctgtt cggcggctta tatgattttg cgggtcaaat cagggaagac aacatttcca
    12181 aaggcggttt tcgttttgcc aacgccatgt atttaaaaga ggctttggtt aaaatcgagc
    12241 agatgcccga gcggactttt gaagaaatca tcgccaaata tgttgaaatg aacattgccc
    12301 atccgttttt ggagggtaat ggcagaagta cccgcatctg gctggatttg gtgctgaaaa
    12361 aaaacctgaa aaaagtcgtg aactggcaaa atgtaagtaa aaccctgtat ttgcaggcga
    12421 tggaacgcag ccccgtcaac gatttagaac tgcgctttct gttaaaggac aacctgactg
    12481 acgatgtgga caaccgtgaa atcatcttta aaggtatcga gcagtcgtat tattacgaag
    12541 ggtatgaaaa aggctgaggg tcgtctgaaa agcgatttca gactgtttca gacgacctga
    12601 ttcggtaggt gatcagacgg gagcggatga gaaaagaaat tctgggtaag aataatccgg
    12661 tctgaaatat tggaagaaga atgatggata aaaatcagtt agaacaagaa tttcataaag
    12721 ccatgttaaa tatttatcag gaggctttga atttgccgca accttacaag gcgacacgat
    12781 ttttacaaat tgtaaatgaa tttggtggta aagaggcggc ggataaatta ttgagtacgg
    12841 gggaaaagaa gactcagacc ggttttacag agctgatttt gagtggtggc ggagtccacg
    12901 ccttgaaata cagtatggaa tatctggtgt tacaaaagcc gtggtgtgat ttatttactg
    12961 aagagcaatt agctgtggca cgcaaacgat tggagcgtgt tggatttgtt tttccgaagt
    13021 aattttgtac gaaacaaaca tagattttta aatcaatcgg attcaatcaa atgaacgata
    13081 tgactttata tttgataatt gcccttgttc cgttggcagg ctcgctgatt gcgggtttgt
    13141 tcggcaacaa aatcggacgt gccggtgcgc atacggttac gatactcggc gtggcggtgt
    13201 ccgccgtgct gtcggcttat gtgctgtggg gctttattga cggcagccgc gccaagtttg
    13261 acgagaatgt ctatacctgg ctgacaatgg gcggcttgga tttctccgtc ggcttcttgg
    13321 tcgatacgat gacggcgatg atgatggtcg tggtaacggg cgtgtcgttg atggtgcata
    13381 tctataccat cggctatatg cacgatgaaa aagtcggcta ccaacgcttc ttcagctata
    13441 tttctttgtt tacattcagt atgttgatgc tgattatgag caacaacttc attcagctct
    13501 tcttcggttg ggaagcggtg ggcttggtgt cgtatctctt gatcggtttc tatttcaaac
    13561 gcccgagcgc gacatttgcc aacctgaaag cctttttgat caaccgtgtc ggcgacttcg
    13621 gctttttgct cggtatcggc ttggtgcttg cctatttcgg cggcagcttg cgctatcaag
    13681 atgtattcgc ttatctgccc aacgtgcaaa atgccactat ccaactgttc cccggtgtgg
    13741 aatggtcttt gattactgta acctgtttgc tcctgtttgt cggtgcgatg ggtaaatcgg
    13801 cacaattccc gctgcacgtc tggctgcctg attcgatgga aggcccgacc ccgatttctg
    13861 cattgattca cgccgcaacc atggttaccg ccggtttgtt tatggtgtcg cgtatgtcgc
    13921 cgatttatga aatgagcagc accgcgctgt cggtcattat ggtgatcggc gcgattaccg
    13981 ccctgtttat gggctttttg ggcgtgattc aaaacgacat caaacgtgta gttgcgtatt
    14041 ccaccctgtc gcaattgggc tacatgaccg tggctctggg cgcgtctgcc tattccgtgg
    14101 cgatgttcca tgtgatgacc cacgccttct ttaaagccct gttgttcttg gcggcaggca
    14161 gcgcgattat cggtatgcac cacgaccaag acatgcgcca tatgggcaat ctgaaaaaat
    14221 atatgccggt tacttggctg accatgctga tcggtaactt gtcgctgatt ggtacgccgt
    14281 tcttctccgg cttctactcc aaagattcga ttatcgaagc ggcgaaatac agcacactgc
    14341 cgggcagcgg ctttgcctat tttgccgtcc tcgccagcgt gtttgttacc gcgttttacg
    14401 cgttccgcca atactttatg gtgttccacg gcgaagagaa atggcgcagc ctgcccgaac
    14461 accattcaga cggccacggc gaagaacatc acggtttggg taaaaacgac aatccgcacg
    14521 aaagcccgtt ggtggttacc ctgcctttga ttttgcttgc cgttccgtcc gtcatcatcg
    14581 gctacatcgc catcgaaccc atgctctacg gcgatttctt caaagacgtg attttcgtca
    14641 acgccgacgc gcatccgact atacacatca tgaaggaaga gttccacggc gcattggcaa
    14701 tggtgtccca cagcctgcat tcgcccgtac tctaccttgc tatcgcaggc gtgttgagcg
    14761 catggctttt gtacgtcaaa ctgccgcacc tgccagcgaa aattgcacag acgttccgtc
    14821 cgatttacgt tttgtttgaa aacaaatact acctcgacgc cctgtatttc aacgttttcg
    14881 ccaaaggcac acgcgcattg ggcactttct tctggaaagt cggcgatacc gccattattg
    14941 acaacggtat tgtcaacggc tctgccaaac tggtcggcgc gattgccgcg caagtgcgta
    15001 aagcccaaac cggctttatc tacacctacg ccgccgctat ggtgttcggc gtattggtct
    15061 tgctcggcat gaccttctgg ggattgttcc gataagaata aggtttcaga cggccttaaa
    15121 ccttcaggcc gtctgaaacg aagaaatatc cacataaaca catttttatt ttaaccacag
    15181 gttaaccact atgttttcca actacctact cagcttggca atatggatac ccatcgccgc
    15241 aggcgtgctg gttttggcaa cggggtcgga cagccgtgcg ccgtttgccc gcgtgctcgc
    15301 cttcatgggt gcgcttgccg gtttcttggt aacactgccc ctgtttaccg gtttcgaccg
    15361 tttgagcggc ggctatcaat ttaccgagtt ccacgagtgg attccgcttc tgaaaatcaa
    15421 ctacgcattg ggcgtggacg gtatttcagt gctctttatc atcttgaatg cgtttattac
    15481 gctgttggtg gtattggcag gttgggaagt cattcagaaa cgtccggcgc agtatatggc
    15541 ggcattcctg atcatgtcgg gtttgattaa cggcgcgttt gccgcgcagg atgcgattct
    15601 gttttatgtg ttcttcgagg gtatgctgat tccgctgtac ctgattatcg gtgtatgggg
    15661 cggtccgcgc cgcgtctatg cgtcggtcaa gctcttcctc tacacgctga tgggttcgct
    15721 cctgatgctg gttgcgatgg tttaccttta ttatcaaaca ggcagcttct ctattgtcga
    15781 tttccaaaac atcgaacaga ttccgttggg cgtacaacag cttttgtttg tggcgttctt
    15841 cctgtcattt gccgtaaaag tgccgatgtt ccctgtgcac acttggttgc cggatgccca
    15901 cgttgaagcg ccgaccggcg gttcgatggt gttggcggcc attacgctga aactgggtgc
    15961 gtatggtttc ttgcgcttta tcctgccgat tatgccggat gcggcacgct attttgcccc
    16021 cgtgatcatc gtattaagtc tgattgccgt gatttatatc ggtatggtgg ctttggtgca
    16081 aaccgatatg aaaaaactgg tggcgtattc gtccatcagc catatgggtt ttgtaacgct
    16141 tgggatgttt ttgtttgttg acgggcagtt ggacgactgg gcattgaaag gtgcaatcat
    16201 tcaaatgatt tcgcacggtt tcgtgtctgc cgcgatgttt atgtgtatcg gcgtgatgta
    16261 cgaccgcctg cacacgcgca atattgctga ttatggcggc gtggtcaatg tgatgcccaa
    16321 gtttgcggcg tttatgatgc tgttcggtat ggcgaacgcg ggtttgcctg cgacttccgg
    16381 cttcgtgggc gagtttatgg tgattatggg cgcggtcaaa gtgaatttct gggtcggcgc
    16441 gttggccgcc atgaccctga tttacggtgc atcttatacc ctgtggatgt acaaacgcgt
    16501 tatttttggt gcgatccaca atccgcacgt tgccgaaatg caagacatca attgccgcga
    16561 atttgcgatt ttggcaattt tggcggtggc tgttttgggt atgggcctgt atccgaacgc
    16621 atttatcgaa gtggtgcatc aggcggcaaa cgatttgatt gcccatgtgg cacaaagcaa
    16681 gatttgaggt gtgtaaatga actggtctga tttgaattta atgcccgcca tgcccgaaat
    16741 cgtgctgctg tcgctgctgg tgttattgtt gctggcggac ttgtgggtca gtgatgacaa
    16801 acgccggtgg acgcattacg gcgcgttggc aacggtggcg gttacggctg tggtgcagtt
    16861 ggcggtgtgg gaacagggca gcacgtcttc gttcaacggg atgtatattg cagacggtat
    16921 gtcgcgtttg gcaaaaatgg ttttatatgc cttgaccttt gccctgtttg tctatgccaa
    16981 gccctacaac caagtgcgcg gtatttttaa aggcgagttt tacaccctgt cattgtttgc
    17041 cctgttgggt atgagtgtga tggtgagcgc ggggcatttt ttaactgcct atatcggttt
    17101 ggaactcttg tcgcttgccc tttacgccct gattgccctg cgccgcgatt ccggctttgc
    17161 cgccgaagcc gccttgaaat attttgtttt gggcgcgctg gcatccggcc tgctgctcta
    17221 cggtatttct atggtttacg gcgcaaccgg ttcgctggaa tttgccggcg tgctcgcctc
    17281 ttccttcaat gaagaagcca acgaatggct gttgaaactg ggtttggtgt ttatcgtcgt
    17341 cgccgtcgcg ttcaaactcg gtgcggtgcc gttccatatg tgggtgcccg acgtgtatca
    17401 cggcgcgccc acttctgtta ccgccttggt cggcactgcc ccgaaaatcg ccgccgtcgt
    17461 tttcactttc cgcatcctcg ttaccgggct gggaaccgtg catcatgact ggtctctgat
    17521 gtttgccctg cttgccgccg cctcgctgct ggtcggcaac cttgccgcca tcatgcagac
    17581 caatatcaaa cgtatgttcg cctattccac cgtatcgcat atgggtttca tcctgttggc
    17641 gtttatggcg ggcgcggtcg gctttgcggc gggcctctat tacgccatta cctacgcgct
    17701 gatggcggcg gcagggttcg gagtgttgat ggtgttgtcg gacggggaca acgagtgcga
    17761 aaacatcagc gatttggcag ggttgaacca acaccgcgta tggcttgcct ttttgatgct
    17821 gctggttatg ttctctatgg cgggcattcc gccgctgatg ggtttttacg ccaaattcgg
    17881 cgtgattatg gcactcttga aacaaggcca tgtttggttg tctgtatttg ccgtcatcat
    17941 gtcgctgatt ggtgcgttct actacctgcg cgtggtcaaa gtcatctact tcgatgtgcc
    18001 tgatcatgac cagccggtcg gcagcaacta tgccgccaaa tttgttctga cggtcaatgc
    18061 cttcttgctg ctcctgtggg gcatcatgcc gcaaaccgtt atcgactggt gcgccaaggc
    18121 gttggagaac acgctgtaag ccgccgcaac ggcagccgtg tcagaggctg ccgtttttgt
    18181 taagatatgc cgttccgcaa cgcggttcag acggcatcgc cgccgacaac gcctaaacag
    18241 aaagcccacc atgaccgcat ccatgtacat ccttttggtc ttggcactca tctttgccaa
    18301 cgcccccttc ctcacgacca gactgttcgg cgtggccgca ctcaagcgca aacatttcgg
    18361 acaccacatg atcgagctgg cggcaggttt cgcgctgacc gccgttcttg cctacatcct
    18421 cgaatcccgt gcaggatcgg tacacgatca gggttgggag ttttatgcca cagtcgtctg
    18481 cctgtacctg atttttgcgt ttccatgttt tgtgtggcgg tatttttggc acacgcgcaa
    18541 cagggaatag acaagcatag gaatgccgtc tgaaaccctt tcagacggca tttgtttcat
    18601 tcaagtgcag gccggcatcg ctgtgccggc acgtttcagc cggcgatata cgccggtttt
    18661 aatatttgcg ggcgactgca aattctgcca actgccgcag gcgcagggct ttgtcgccga
    18721 agggttcgag cagcgcgacc gcttcggcaa ccagtttgtg tgcgtatgag cgcgccgctt
    18781 ccaagcccat cagtttcaca taagtcggct tgtcgttgtc tgcgtctttg cccgccgttt
    18841 tgcccaaagt cgccgtgtcc gcttcacaat ccaacacatc gtcaatgact tggaacgcca
    18901 gccccagttt tgccgcgtaa gcgtccaata cggaaagttc cgcatctgac agatcaggac
    18961 acgccgtcgc ccccaataaa accgccgcac ggattagcgc acccgttttc aggctgtgca
    19021 tctgttccaa atcggcttga accatttgtt tgccgacatt cgccaaatcg attgcctgac
    19081 cgcccgccat acccctgctg ccgcccgctt tcgccaacac cgacaacatt gccaactggc
    19141 gtgcggcggg cagttctgtc ggacggctca acacgtcaaa tgcctgtgtc tgcaaagcgt
    19201 cgccggtcag aagggcggtc gcttcgccat atttgatgtg gcaagtcggt ttgccgcgcc
    19261 gcaggctgtc gttgtccatc gccggcatat cgtcgtgaac caaagaatag acgtggatca
    19321 tttcgattgc cgccattgcc tgttctactg cttcatgcac ggcttcgcct aattccgaag
    19381 ctgccagaac cagcatcggc cgcagacgct taccgccgtc caaagccgca taacgcatcg
    19441 cttcgtgcag tgtgtgcggt atttccccct cagacggtaa aaaccgttca agcagcagct
    19501 ctgtttgcgc ctgcgccctc tgttgccacg ttttcaaatc attcgtcgga ttcaaggttt
    19561 aactccttca gcccgtctgt gtctaaaacc tgtagctttt gttcgacttg tgccagtttg
    19621 gtttggcagt acctgaccag ttcgttgcct tcctgatagg cggcaagcgc gtcttccaag
    19681 ggcatttcgc cctgcataga ctgcgtcagc gattcgaggc gcgacaaggc ttcttcaaac
    19741 gatttcgggg cgtttttctt catcgtattt ccttttcggt tgaaaccccg ccctttaggg
    19801 cggcaggatc agactttatt tgggaggggt gtaacccttt ccaaatcagg gcaatacata
    19861 gggcggtgct ttatgtgccg tcctgtgtgt tggaacatag tttcggatgt tccggtaaaa
    19921 agcggattgt agcatttttg aaaaacggat gccgtctgaa acccgaatcc ggcttcagac
    19981 ggcatttttt ccgcccaggc ggcaaggcgt tacccgggca gttcgtcggt gatgccctgc
    20041 aaaaaggcga ggcgttcggg gcttgccgcc ccggtttgcg cggcggcttt gaaggcgcag
    20101 ccgggttcgg cgcggtgggt gcagttgtgg aagcggcatt gcccgacaag gtggcggaaa
    20161 tcggggaaat agcgcggcaa atcggcggct tggaggtggt gtaaaccaaa ttcttgcaaa
    20221 cccggggagt cgatgagttg ggtttcgccg ttcaaatcat aaagccgggc gtgggtggtg
    20281 gtgtgttttc ccgagtcgag tgcggcggaa atgtcgccgg tgcgggcggt ttggctgccc
    20341 aaaagggcgt tggtcagggt ggatttgccc ataccgctct gcccgagcag gatgttgctg
    20401 tgcccttgca gggcggggcg caggctgccg gcgttttcca gtgcgcgggt ttcgatgacg
    20461 ggataaccca gcgtttcgta gaatttgagt ttttcgcgcc aaagggcggt ttcgggcagg
    20521 tcggctttgt tcaggacgat gacggcttca ataccggcgg cttcggcggc aagcagggcg
    20581 cgttgcagca gccgcacgct cggactcggg acggcggcgg ttacgatgag gagttgggta
    20641 acgttggcgg cgatgagttt ggttttccac gcgtcttggc ggtagagcag gctttggcgc
    20701 ggtaaaaaat cttcaatcac aacttgttcg gcgttgacgg ggctgatgcg gacgcggtcg
    20761 ccgcaggcga aatcgacgcg ttttttgcgg gtgctggctt cgtaggttgt gccgtcgggc
    20821 gtgcggacaa tgtagcggcg gccgtagctg gcggtaattt gggcggtgtc gttcatggtt
    20881 tctttggggt tgggtgtggg aatgccgtct gaaaacgggt gttcggacgg catcggttca
    20941 gtcgtgctgc cactcgacgt gttcgttgag gaagccgccg ctctggtgcg cccagagttt
    21001 ggcgtaaagc ccgcgttttt cgaggagttc ggcgtgtgtg ccttcttcga tgatgcggcc
    21061 tttgtcgagg acgacgagcc tgtccattgc ggcgatggtg gagaggcggt gggcgatggc
    21121 gatgacggtt ttgccgtcca tcattttgtc gaggctttct tggatggcgg cttcgacttc
    21181 ggaatcgagc gcgctggtgg cttcgtccaa aagaagaatc ggtgcgtctt tgagcatcac
    21241 gcgggcgatg gcgatgcgct ggcgttgccc gccggagagt ttcacgccgc gttcgccgac
    21301 gtgtgcgtcg tagccgcgcc gccctttggc atcggaaagg tcggggatga agccggcggc
    21361 ttcggcgcgt tcggcggcag aaaccatttc ggcatcggtc gcgtcggggc ggccgtaaat
    21421 aatgttgtcg cgcacggaac ggtgcagcag cgaggtatct tgcgtgacca aaccgatttg
    21481 ggcgcgtaaa gattcttggg taacgccgct tatgtcctgc ccgtcgatcg aaaccgtgcc
    21541 gctttgcggt tcgtagaagc gcaaaagcag gttgacgatg gtggatttgc ccgcgccgct
    21601 gcgtccgatc aagccgactt tttcgcccgg gcggatggtg aggttgaagc cgttgagcag
    21661 cggtttgccc gcttcgtagg agaaatcgac gtgttcaaat ttgattgcgc cttgcggcac
    21721 gttcagcggc agtgcccggg gcttgtcgag gatggtgtgc ggtttggaca gggttgccat
    21781 gccgtcgccg acggtgccga tgttttcaaa cagccgcgcg gattcccaca taatgtattg
    21841 cgacaaaccg ttgacgcgca acgccatggc ggtggctgta gcaaccgcgc ccacgccgac
    21901 ctgcccgttg tgccagagcc agatgcccag tgcggcggtg gagagggtca gggaggtgtt
    21961 gacgatgaag ctgcacgaat gcagcagcgt cgccagccgc atttgggcgc gcaccgtaac
    22021 cataaattct tccatcgact gcttggcata ggcggcttca cgcgcgccgt gggagaagag
    22081 tttgacggtg gcgatattgg aataggcatc ggtaatgcgg ccggtcatca gcgagcgggc
    22141 atccgcctgc catgcggcgg tttgccccaa tttgggaatc agcaggcgca tcaccgaagc
    22201 gaaaccgaca atccagccga taaagggcag cagcagccat gagtcgagcg aggcgagaat
    22261 cacgccggag gtaatgaaat acaccgacac ataaacgacc atatcggcaa ccgtcatcac
    22321 cgcgtcgcgc aacgccagcg cggtctgcat gactttggcg gacacgcgtc cggcaaattc
    22381 gtcctgataa aaaccgaggc tttggttcag catcaggcgg tggaagttcc agcgcaggcg
    22441 catggggaac acgccctgaa gggtttgcag gcgcacgttg gacgcggcaa acgcccacgc
    22501 aaccgaaaat accatcatcg ccgccattgc cgccagttcc caaccttttt cggcaaacag
    22561 ttcggcgggc gcgtatttgc cgagccactc cacgattttg cccataaatt gaaaaaccag
    22621 ggcttccata atgccgatgc cggcggtcag cgcagccagg gcggctatcc atttccgcac
    22681 gccggccatg ctgctccaga caaaccgcca caagcctttt tctggcgttt tcggggcggc
    22741 ttcgggataa gggtcgattc gggactcgaa ccaggaaaat attttgttca acattgtttt
    22801 cgatttcggt aaaacagttt cagacggcat caaacacaat gccgtctgaa aggaaggaca
    22861 ataacgccat tttacgggaa aagccgtcgg gaagacagcg cgaggcggaa acgcagggtt
    22921 tcgtcagggc aaacgccgcg ccgccttcag gcggcattat ttcagcaggt ttttcaaagc
    22981 aaggcgcacg ccttcgccca cgtccgtccc ctccggaacg cctttgaccg ccgcttttgc
    23041 ttcgcgttcg ctgtaaccca gcgcaagcag cgtgctgacg atgtcttccg tttcgtcggc
    23101 ggcgggtgcg gcggcaaaca gcccgtccgt taccgtatgc gcgaccagct tgccgcgcag
    23161 ttccaaaacc atacgttcgg cggttttttt gccgattccc ggggcggagg agaggcgttt
    23221 gacatcttct tctgcaaccg cccgcgccag ttcgtcggca gtcattgccg acaaaatgcc
    23281 caaagccgtt ttcgcgccga tgccgccgac cttgatcagt tggcggaagg tcttgcgttc
    23341 ttccgcagtg gcaaaaccaa ataaaagatg tgcgtcttcc cgaatgataa gctgggtaaa
    23401 cagttgtacg ctttcaccca cgggcggcag gttgtagaag gtctgcatcg atacgtcggc
    23461 ctcatagccg acaccgttga catcgatgac gatttgcgga gggttttttt caaccagttt
    23521 gccggtcagt ctgctgatca tgtgtgccga atcctgaagt gtcgggtgca aaatgccgtc
    23581 tgaaaccggt ttgggcttca gacggcacgg attgtatcaa attcagtcgt cgcggcggga
    23641 ggaaatcacg cggccggtac gggcatcgac aacgactttg tattcctgtc cgtttttgac
    23701 gatttcgaca tcatagtgcg gacggccgtt gtcgtgttcg agatcgatgt cggtgatttt
    23761 gccgccgaca cgcgccaacg ctgctttttc ggcttgggcg cggctgatga ttttgtcttg
    23821 tttgttgtgt tggtgtgcgg cgtgtccgtg gtcgtcatcg ccgtgtccgt cgtggtgggc
    23881 gagcgcgggg gcggaaatgc tcagcagtgc ggttgcggcg gaggtcaaga gaaggtgttt
    23941 gatgttcata ttttgccttt gtaaatcgtg ggttggaaaa tgtggatatt aataaggtat
    24001 caaataaccg tcagccggcg gtcaataccg cccgaaccat accgcgcgcc tgagcttcgg
    24061 cttcggcggc gcgttcctgc gaggtaaacg gtcccatttt gacgacgtat tcgtaacggc
    24121 gtttttcaac cgagaggttc gtacccgatg acgaaacggc gaagttttgg gcggcttggt
    24181 tcagataggc ttgtgcttcg tgttccgtac cgaaagattt caagtcgata aagatgtctt
    24241 tgttttcggc aaccggtgcg gattggcccg ggacgatttg ttcgattttg acgtgtgccg
    24301 tcccttggtt gacaaagccc aatttttgcg cggcggcttt ggatacgtcg atgatgcggt
    24361 tgccgtggaa ggggccgcgg tcgttgacgc ggacgatgac gcttttgccg tttttggtat
    24421 tggttacgcg cacatagctg gggatgggca gggttttgtg ggcggcggta aaggcgttca
    24481 tatcgtatcg ttctccgccg gaagttttgc gcccgtgaaa cctgccgccg taccacgagg
    24541 cgttgccggt ttgcgtgaat tcggcgactt ggtttttcgg cgtgtagcgt tttccggcga
    24601 ctttgtagct gcggttggcg gaggcgtgca gtttttctgc cttgaccact gcgtcggcgg
    24661 atgccgtctg aagggagtgt gtgccgaatg cggcggtgag aaggaaaagg gtttttcggg
    24721 ttaaagtcaa aacgtgttcc gttcttgagt tgaagacgaa tgggcatcat gcccgccgga
    24781 tacgttccga accgccgtac agtgcggacg gcggttcgga atgtgtccgg ataggttttc
    24841 agacggcatg aacctgcgtt caaacgccgc ctgcgtaacc gtgttgccgc cacgcttcaa
    24901 agagaatcac ggcgacggtg ttggaaaggt tcatactccg gctgccgggc tgcatcggca
    24961 ggcggatttt ttgcgcggcg ggcaggctgt cgaggatgtc ggcaggcagt ccgcgcgttt
    25021 ccggcccgaa cagtaaaacg tcgccttttt gaaacgcggt ttcatcgggg cgcgccgtgc
    25081 ctttggtggt cagggcgaaa atgcgcctgc ctgcgagtgc cttgaggcag tcgtcgaagt
    25141 tttcgtgcac cgtcaggctg gcgaactcgt ggtagtcgag cccggcgcgt ttcattttgg
    25201 cggaatccaa tgggaagccg agcggtttga caaggtgcaa atccgcgccg gtattggcgc
    25261 acaggcggat gatgttgccc gtgttcggcg ggatttccgg ctggtataaa acgatggtaa
    25321 acataaatat caatcactta taggcgcgta accttgccac aaggcggatg gggtgtcaaa
    25381 aaatttagtt attttttcat tggcgtgcgt gccagcgtcc agcagcagat tcggtttgcg
    25441 cccgattttt tcagcgtctt tgccaattcg tccagcgtcg cgccggtggt aaagacatcg
    25501 tcgattaaca gaatattaca gttttccggt atcggtgtgc ggatttcaaa ggcgtttttg
    25561 atgtttcgcc gccgttcgcc gcctttgagc gtgctttgcg gcgggcggtg gtgtcggaaa
    25621 acggtgtgtc ggggcagtat ctgccagccg tagcgttgtg ccagcagccc gacgatgctt
    25681 tcactttggt tgaacccgcg ttgcagcagc cgctccctgc ttagcggtac gggcaggacg
    25741 aaatcgaaac attcgtctgc aagccggtcg ggcggattct gcatcatcag gtctgccagc
    25801 ggctgcacca tgctcaaatc agccaagtgc ttcagcgcgt gtatcatatt gctgacgggc
    25861 ggttcgtaat gcagcgaagc ccacatccgg tcgaatgcgg gcggtttttt ctgacagccg
    25921 ccgcacaccg atccgccttg gatgtgtctg aaacacaggg ggcagctgtt tgccgcgtcg
    25981 gtgcggtatg ccgccaaatc gtcgcggcag ccggcgcaga tgccgtctga aacgccagac
    26041 gaaccgtggc ataatacgca acgcctgata gtgggcgcgt ctgcgatgcg ccgccaacga
    26101 gagagaaaat ccatgcctga tgccgtcaaa aaagtttacc tgatacacgg ttggggggcg
    26161 aaccgccaca tgttcgacga tttgatgccg cgcctgcctg caacgtggcc ggtgtccgcc
    26221 gtcgatttgc ccggacacgg ggacgctccg tttgtccgac ctttcgacat tgcggctgcg
    26281 gccgacggca ttgccgctca aattgacgct ccggccgaca ttctcggctg gtcgctcggc
    26341 ggattggtcg cgctgtatct ggcggcgcgc catcccgaca aagtccgttc gctctgcctg
    26401 acggcgagtt tcgcacggct gacggctgac gaagactatc ccgaagggct tgccgcgcct
    26461 gcattgggca aaatggtcgg tgcgttccgt tcggattatg ccaaacatat caaacagttt
    26521 ctacaattac agcttctgca cacgcctgat gcggacggaa tcataggcag aatcctgccc
    26581 gatttggcgc gctgcggcac gcctcaagcc ttgcaggagg cgttggacgc ggcggaaagg
    26641 gcggatgcgc ggcatttgtt ggacaagata gatgttccgg tactgctggt gttcggcggc
    26701 aaagacgcga ttacgccgcc gcgtatgggt gaatatctgc accgccgttt gaagggcagc
    26761 aggttggttg tgatggaaaa ggcggcgcat gcgccgtttt tgagccatgc ggaagcgttt
    26821 gccgcgctgt accgcgactt tgttgaaggg ggtttgagat gaaccatcag gacgcacgct
    26881 ggcaggttca ccgccatctt gccgaacata ccgaccaacg gctgacactc gtccgcaacg
    26941 cgcccaagca tatcctgctt gccggtgcgg atgcggacat cagccgcagc ctgctggcga
    27001 aacgctatcc gcaggcggta tttgaagaat acgattcccg tgcggatttt ttggcggctg
    27061 ccgctgccgc ccgcaaaggc ggtttttggc aaaggtttac gggtaagggc gtggtgcaac
    27121 actgccaatc cccgatcgcg ccgctgcccg aagcgtgtgc cgatatgttg tggtcgaatc
    27181 tcggactgtt ggcggcggaa caaatccttc ctgtgctgca caactgggcg cgcgccttga
    27241 agacggacgg gctgctgttt tttacctgct tcgggcgaga taccttggcg gaactgaaat
    27301 gccgtctgaa agaaaacggc attgaaagcc gcagcgcgct tttccctgat atgcacgact
    27361 tgggcgatat gcttgctgaa aacggctttt acgaccccgt taccgatacg gcgaagctgg
    27421 tgttggatta caaaaaggcg gaaacgtttt gggcggatat ggacacgctg ggcgtttggc
    27481 gggcgatggc gtggaacgat gaaaacgccg cgcgttcgtg tgtcgggaca atatttgagc
    27541 gggaaggcgg tttgggcatt acgctggaaa cggtgtacgg acacgccgtg aaaaaactga
    27601 tgctgccgca aggggagaac gtggtgcagt tttttccgaa gagatgatgt gcagatgccg
    27661 tctgaagccg tttccaggtt tcagacggca tttgtctgtg aaaaccgaca gaaataaagg
    27721 aaatgccgat gtatagtg
//
LOCUS       DAWIVB010000007        26431 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWIVB010000007 DAWIVB010000000
VERSION     DAWIVB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.007"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            719..2167
                     /gene="gnd"
                     /locus_tag="WIX60_000214"
     CDS             719..2167
                     /gene="gnd"
                     /locus_tag="WIX60_000214"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ5304545.1"
                     /translation="MNGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTTAKVDEFLNG
                     AAKGTNIIGAYSLQDLVDKLEKPRKIMMMVRAGSVVDEFIEQLLPLLEEGDILIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWDAVKPIFQAIA
                     AKTSQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMYRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLGNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2230..3501
                     /gene="waaA"
                     /locus_tag="WIX60_000215"
     CDS             2230..3501
                     /gene="waaA"
                     /locus_tag="WIX60_000215"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ5304546.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMRECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGYRGR"
     gene            3542..3991
                     /locus_tag="WIX60_000216"
     CDS             3542..3991
                     /locus_tag="WIX60_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ5304547.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4024..4995
                     /locus_tag="WIX60_000217"
     CDS             4024..4995
                     /locus_tag="WIX60_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ5304548.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNTVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASVLTSLVYTKKTGR"
     gene            5069..5144
                     /locus_tag="WIX60_000218"
     tRNA            5069..5144
                     /locus_tag="WIX60_000218"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5102..5104,aa:Lys,seq:ttt)
     gene            5515..6768
                     /gene="murA"
                     /locus_tag="WIX60_000219"
     CDS             5515..6768
                     /gene="murA"
                     /locus_tag="WIX60_000219"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ5304549.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELQGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6849..8027
                     /locus_tag="WIX60_000220"
     CDS             6849..8027
                     /locus_tag="WIX60_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ5304550.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGSLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(8094..8342)
                     /locus_tag="WIX60_000221"
     CDS             complement(8094..8342)
                     /locus_tag="WIX60_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ5304551.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8433..9350)
                     /gene="ftsX"
                     /locus_tag="WIX60_000222"
     CDS             complement(8433..9350)
                     /gene="ftsX"
                     /locus_tag="WIX60_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ5304552.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLMMLAVAMTLPLF
                     MHLGIQSGQSVLGKLNESPQITIYMETSAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYAGELG
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9347..9997)
                     /gene="ftsE"
                     /locus_tag="WIX60_000223"
     CDS             complement(9347..9997)
                     /gene="ftsE"
                     /locus_tag="WIX60_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ5304553.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTI
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10191..10673)
                     /locus_tag="WIX60_000224"
     CDS             complement(10191..10673)
                     /locus_tag="WIX60_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ5304554.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTDAVKLAHSKCR
                     "
     gene            10797..11150
                     /gene="arsC"
                     /locus_tag="WIX60_000225"
     CDS             10797..11150
                     /gene="arsC"
                     /locus_tag="WIX60_000225"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ5304555.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11147..11791
                     /locus_tag="WIX60_000226"
     CDS             11147..11791
                     /locus_tag="WIX60_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ5304556.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFN"
     gene            11996..13390
                     /locus_tag="WIX60_000227"
     CDS             11996..13390
                     /locus_tag="WIX60_000227"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ5304557.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGSATYDRRWRPEVGKTLPEIPSDVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIDANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLELVKDRPQDLNTLADECFYFYVKQTPAEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13411..13806
                     /locus_tag="WIX60_000228"
     CDS             13411..13806
                     /locus_tag="WIX60_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304558.1"
                     /translation="MPSDVGIRLQTAFKWKLKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEDLRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13810..14301
                     /locus_tag="WIX60_000229"
     CDS             13810..14301
                     /locus_tag="WIX60_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ5304559.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGILLGSAAVKRHDMESFPRYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTSDVQIFYESFGWVVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14425..17019)
                     /gene="mutS"
                     /locus_tag="WIX60_000230"
     CDS             complement(14425..17019)
                     /gene="mutS"
                     /locus_tag="WIX60_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ5304560.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARIAVGNARPRDLAALRDSLFALSEIELSAECSSLLGTLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHTAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17463..18467
                     /gene="gap"
                     /locus_tag="WIX60_000231"
     CDS             17463..18467
                     /gene="gap"
                     /locus_tag="WIX60_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ5304561.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVASGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18537..18656)
                     /locus_tag="WIX60_000232"
                     /pseudo
     CDS             complement(18537..18656)
                     /locus_tag="WIX60_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18725..19405)
                     /gene="tsaA"
                     /locus_tag="WIX60_000233"
     CDS             complement(18725..19405)
                     /gene="tsaA"
                     /locus_tag="WIX60_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ5304562.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAKACIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19519..20154
                     /locus_tag="WIX60_000234"
     CDS             19519..20154
                     /locus_tag="WIX60_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ5304563.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            20191..21159
                     /gene="waaC"
                     /locus_tag="WIX60_000235"
     CDS             20191..21159
                     /gene="waaC"
                     /locus_tag="WIX60_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ5304564.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21386..22135
                     /locus_tag="WIX60_000236"
     CDS             21386..22135
                     /locus_tag="WIX60_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ5304565.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22146..23081
                     /locus_tag="WIX60_000237"
     CDS             22146..23081
                     /locus_tag="WIX60_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ5304566.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGNGASAVV
                     EFAKQVAGVKKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            23144..23755
                     /locus_tag="WIX60_000238"
     CDS             23144..23755
                     /locus_tag="WIX60_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ5304567.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(23807..24124)
                     /locus_tag="WIX60_000239"
     CDS             complement(23807..24124)
                     /locus_tag="WIX60_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ5304568.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24282..25553
                     /gene="purD"
                     /locus_tag="WIX60_000240"
     CDS             24282..25553
                     /gene="purD"
                     /locus_tag="WIX60_000240"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ5304569.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDIISGLDAANQIGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25587..26189
                     /locus_tag="WIX60_000241"
     CDS             25587..26189
                     /locus_tag="WIX60_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ5304570.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         6348 a         6087 c         7434 g         6562 t
ORIGIN      
        1 cggagcggtt tctgttgctc ccgataaatt cctaaaactc aaaatttcat cattcccact
       61 ttcgtgggaa tgacggaatg taggttcgtg ggaatgacgc ggtacaggtt tccgttacgt
      121 atccccatcc gaaactttac ttatacacaa gcaatccaaa gttatctgac tgaaatattg
      181 tgagatttac ccgcaaatct cattttttcc accacttata cacaaattcg gatgcgcact
      241 ctgccgatat gcagaaataa gacattcgtg ccgacacctg accatttcca caagttccaa
      301 acacactttc ctctcaaacc tccacagtat tccggttctt aaagacatcg gcagccattc
      361 ggtaaagaaa agtaggatgc catttgcaca acaagcaaac tgtccacaaa aactattctt
      421 catgaaaaac aatccacagg tatgcacaaa agggaatccg ccttaccgac accctaaaaa
      481 aaagaaatcc tgatgatttt taaaataaaa aaaagttacc cacagaaaat ttctcctatc
      541 aatataatca tcgttttatt atttaaattt tatattttat ttataaccct tcagcaaaac
      601 ggtacagcaa agcgtaaagg catcttttca aaaaattaaa aagttaagga ttgttaaaca
      661 agtgtaaatc gggtagagtt gcggtcgata aattaaatct ataacggagc ttcctgcaat
      721 gaacggcgat attggtgtaa tcggtttggc ggttatgggg caaaacctga ttttgaatat
      781 gaacgattgc ggatttaagg tggttgccta caaccgcacg actgccaagg tggacgaatt
      841 tttaaacggc gcggcaaaag gaactaatat tatcggcgcg tattctttgc aagatttggt
      901 cgataagctg gaaaaaccgc gaaaaattat gatgatggtt cgtgccggtt cggtggttga
      961 cgaatttatc gaacaactgc ttccgctgtt ggaagaaggc gacatcctta tcgacggcgg
     1021 caatgccaat taccccgaca cgacacggcg gacacattac cttgctgaaa aagggatttt
     1081 gtttgtcggc gcgggcgtgt ccggcgggga agaaggggcg cggcgcgggc cgtccattat
     1141 gccgggcggg gataagcgcg cttgggatgc ggtcaagccg atttttcagg cgattgccgc
     1201 caaaacgtcg cagggcgaac cgtgttgcga ctgggtcggc aaagacggtg cggggcattt
     1261 tgtcaaaatg gtgcataacg gcatcgaata tggcgatatg cagttgattt gcgaagcgta
     1321 ccagttcatg aaagacggct tggggctgtc ttacgacgaa atgtaccgcg tgtttgccga
     1381 gtggaacaaa accgagctgg attcttatct gattgaaatc acagcggcaa ttttgggcta
     1441 taaggacgaa ggcggcgaac cgctggtcga gaaaatcctc gatacggcag ggcaaaaagg
     1501 tacaggcaaa tggacgggca tcaatgcttt ggatttgggt attccgctga cgctgatttc
     1561 cgaagccgta ttcgcccgct gcgtttcgtc gttcaaagaa cagcgcgtgc agaccggcaa
     1621 actgttcgcc cgaaccgtta cgcccgtcga aggcggcaaa caagaatggg tcgaggcgtt
     1681 gagacaggcc ctgcttgcat ccaaaatcat ttcctacgca caaggcttta tgctgatccg
     1741 cgaagcgggc gaaagttacg gctgggattt ggactacggc aacactgcgc tgctgtggcg
     1801 cgaggggtgc atcatccgca gcgcgttttt gggcaatatc cgcgacgcgt atgaaaacaa
     1861 tcccgatttg gtgttcttgg gtgcggatcc gtatttcaaa aatattttgg aaaactgcct
     1921 gccggcatgg cgcaaggtgg ttgccaaggc ggtcgaatgc ggcatcccta tgccctgcat
     1981 ggcttcggcg attacgttct tggacggcta cacgaccgaa cgtctgcctg ccaacctctt
     2041 gcaggcacag cgcgactact tcggcgcgca cacttacgaa cgcaccgaca aaccgcgcgg
     2101 cgagtttttc cataccaact ggacgggcaa gggcggagat accgcttcga caacctacga
     2161 tatttgattc cgttgccgaa atgccgtccg aaccgttcgg acggcatttg ttttgcagga
     2221 aatagaaaga tgttccaatg gctttatgat gtattgtggc tgcttgcgcc gatatggata
     2281 cggcgttatt tggacaaacg ctccggaagt gccccggcat atcgggcgca tcgggacgag
     2341 cgtttcggca agccgtatcc gaatcccgtt accggagcgg tttggataca cgccgtttcg
     2401 gtcggggaaa cgcgtgccgc ccaacctttg atacgcgagt tgcggcagcg ttttcccgat
     2461 gcgccgctgc tgatgacgca gatgaccccg acgggacggg aaaccgcgca agttctgttt
     2521 cctgatgcac aatgccgcta tcttccgtat gacaaaaaaa cgtgggtacg gcagtttttg
     2581 cgcgaacacc gcccgatgtt cggcattttg atggaaaccg aaatctggcc caacctgatg
     2641 agggaatgcc ggcgcgcggg tgtgccgctg tttttggcga atgcgcggct gtcggaaaaa
     2701 tcgttgaacg gttatctgaa agtccgccgc ctgatccgtc ctgccgccgc ttcgctgacg
     2761 ggttgtctgg cgcagacgga ggcggatgcg gcgcggttgg cgaaattggg cgcggcatcc
     2821 gtgcaggtgt gcggcaatac caaatacgac ctgatgccgt ctgaagacat gaagacgctg
     2881 gcggggcagt ttgaaaaacg catcggaggc cggccggttg ctgtgtgcgg cagcacgcgc
     2941 gtttatcggg gcgaagacga ggcggaaaaa ctgctggcgg cgtggcaaca atatcgcggc
     3001 gatgccctgc tggtcgtcgt gccgcgccat cccgagcatt ttcagacggt atttgaaacg
     3061 gcaaaacgct tcgggtttaa ggttcagcgg cgcagcgacg gtttgccggt cgaacctgat
     3121 acgcaggtgt ggataggcga cagtatgggc gagctgtatg cgtattacct gtgcgccgat
     3181 gtcgcttttg tcggcggcag tctggtcgat tcgggttgtc agaacatcat cgaaccgctt
     3241 tcctgcggcg ttccgacgat attcggcttt tcaacctaca atttttccga agcctgccga
     3301 cacgccttgg catcgggtgc ggcggttcaa gtcgaatcgg cggatgcgtg gcgggaagcc
     3361 gttgaaaaaa ccttatcgtc cgaggggggg gggatgcaga tgcaggcgcg cgtggacggc
     3421 tttatcgcac aacatcgcgg agcgggcgcg agaatcgccg aggcggtgcg ggaagcggta
     3481 tgcggatatc gggggcgata gtgatacaat ccgtatccga gttttccgtt tggagcatcg
     3541 tatgagtatt tatgccgtcg cgcacatcgt tcatctgtat tgcgctattg cctttgtcgg
     3601 cggcgtgttt tttgaagtgc tggttttgtc cgtcctgcat acgggacggg tgtcgcgcga
     3661 ggcgcggcgc gaagtggaaa aggcaatgtc ttaccgcgcc gtcagggtga tgccgtttgt
     3721 ggtcggactg ctgttcgcca gcggcatcgt gatggcggca aaccgctatc tttctatatt
     3781 gggcgaaccg tttgccactt ccttcggtac gatgctgacg ctgaaaatcc tgttggcgtt
     3841 cagcgtattg gcgcacttcg ccatcgccgt cgtcaaaatg gcgcgttcca cactgacggt
     3901 cggttggtcg aaatacatac acgccgtcgt ctttacccat atgctgctga ttgtcttttt
     3961 ggcaaaagcg atgttttata tcagctggta acacaattac atttcaaatc aggagattga
     4021 accatgacag aatatcccgg catcgggtcg ccgttgtttt acggcgtttt ttttgcggca
     4081 gtgctggtca tgattgcctt ggatatgttt tcgctgaaga aaaacggcag ccataaagtc
     4141 ggcgtcaaag aagccttggc atggagcggc ttgtgggttg ccgtatcctg cctgttcgcg
     4201 ggctggctgt attttgaact cgccggcaat cccggctacg gcgcggcagt cgcaaaagaa
     4261 aaagtattgg aattctttac cggctacatt ttggaaaaat cgttggcagt cgacaatatt
     4321 ttcgtgttcc tgatgatatt cggctacttc aaagtcgcac cacagtttca gcaccgcgtg
     4381 ctgctgtacg gcgtattggg cgcattggta ttgcgcaccg tcatgatttt cgtcggcgcg
     4441 gcactggttc agcagtttga gtggattctg tatctgtttg gcgcgttcct gctctatact
     4501 ggtatacaca tgatgaagcc cgaaggcgat gagaaagagg atttggcaaa cagcaggctg
     4561 ctgaataccg tcaagaaagt cgttccggtc ggcacggaat ttcacggcga gaaatttttt
     4621 accgtcgaaa acggcaaaaa aatcgccacg ccgctgtttt tggtgctggt catgattgaa
     4681 ttgagcgatg tcgtgtttgc cgtggacagc atccccgccg tctttgccgt taccaccgat
     4741 ccgtttatcg tgctgacttc caatattttc gcaattctgg gcttgcgggc gatgtatttc
     4801 ctgctggcgg atgtggcgga acgctttatc ttcctgaaat atggattggc attcgtgttg
     4861 ggttttatcg gtgtgaaaat gctggtaatg cattgggtgc atatcccgat ttccgtttcg
     4921 ctgtcggtcg tgttcggcgc attgggtgca tcggtgttga cttctttggt ttatacgaaa
     4981 aaaacaggcc gataagcgga tataacgccc gtccgcccgc aaggggcttg acagggtttc
     5041 ggccggtctg taaaattcca aacttatcgg gtcgttagct cagccggtag agcagcggac
     5101 ttttaatccg ttggtcgagc gttcgaatcg ctcacgaccc accagataaa gcaaaaaagc
     5161 tgagttttca aaactcggct tttttgtctt ccgacaatcc ttgttcggaa agcgcggcat
     5221 ttcgtcaaat tctttggtgc ggcttccgta tttttcccga aaatccctta taattccgcc
     5281 tctgcaacct tcatcgggtg atgctttatg cgttgtcccg agaattgaaa tattatagtg
     5341 gattaacaaa aaccagtacg gtattgcctc gccttagctc aaagagaacg attctctaag
     5401 gtgctgaagc accaagtgaa tcggttctgt actatctgta ctgtctgcgg cttcgccgcc
     5461 ttgtcccgat ttttgttaat tcactataca agtacccacc aggaaagaac aaacgtggac
     5521 aaactgaaaa tctccgcaaa cggcccgctc aacggggaaa taacggtctc gggcgcgaaa
     5581 aacgcggcat tgccgctgat gtgcgcgggt ttgctgacat cgggtacgtt gcgcctgaaa
     5641 aacgtcccta tgctggcaga tgtgaaaacc acgcaaaagc tgcttcaggg gatgggcgcg
     5701 cgcgtcctga ccgacaatat cagcgaattt gaaatcaacg gcggtacggt aaacaatacc
     5761 tgcgcccctt acgagttggt ccgaacgatg cgcgcttcga ttttggtgct gggcccgacg
     5821 ctggcgcgtt tcggcgaggc gcaagtcagc ctgccgggcg gctgcgccat cggttcgcgc
     5881 cccgtcgatc agcatttgaa aggcttggaa gcgatgggtg ctgagattgt tatcgaacac
     5941 ggttacgtca aagccaaagg caaactcaaa ggtacgcgcg tggcgatgga tgtcgttacc
     6001 gtcggcggca cggaaaacct gctgatggcg gcgacgctgg cggaaggtac gacggttttg
     6061 gaaaactgcg ccattgagcc tgaagtggtc gatttggcgg aatgcctggt caaaatgggc
     6121 gcgaaaatca gcggcatcgg tacgtccaca atgattgtgg aaggggtgga cgagctgcaa
     6181 ggctgcgaac acagcgtcgt ccccgaccgg atcgaggcgg ggacgttcct gtgcgcggtg
     6241 gcgataaccg gtggcagggt ggttttgcgg aatgccgcgc cgaaaacgat ggaagtggtg
     6301 ttggacaaac tggttgaggc aggtgcggtg attgaggcgg gcgacgattg gatcgccatc
     6361 gatatgcggc agcgtccgaa ggcggtggac atccgcacgg tcgtccaccc cggcttcccc
     6421 acggatatgc aggcgcagtt tatggcattg aatgccgtgg cggagggaag ctgccgcgtg
     6481 gtggaaacga tttttgaaaa ccgctttatg cacgtccccg agttgaaccg gatgggggcg
     6541 aacatcacaa ccgagggcaa tacggcattt gtgcagggtg tggaacagct ttccggcgca
     6601 gtcgtcaagg cgacggattt gcgtgcgtcc gccagcctcg ttatcgccgg tttggcggcg
     6661 cgaggcgaaa ccgtggtcga acagatttac cacttggatc gcggttatga aaatattgaa
     6721 aaaaaactcg gcagcgtcgg cgcgaaaatc gagcgcgtat ccggctgatc gggttgcgat
     6781 gccgtctgaa gccttcagac ggcatcaatt gtttccgtat cgcaaacact gattgaaaag
     6841 gactgattat ggcattttta aaactgaccg aacaaaacgt gcagggcaaa accgtcctca
     6901 tccgcgccga tatgaacgta ccgttcaaag acggcaaaat cagcgacgac acccgtatcc
     6961 gcgcctcgct cgcgtccatt aaatactgcg tggacaacgg tgcgtccgtt atcgtgatga
     7021 cccatctcgg ccgcccgacc gaaggcgagt tccaccccga agacgatgtc gcccccgttg
     7081 ccgcgcactt gggcagcctg ttgggcaaag acgtgaaagt attgaacgac tggcgtgaaa
     7141 acaaacccgc cctgaacgcg ggcgatgtcg tcatgctgca aaacgtgcgc atcaacaaag
     7201 gcgagaagaa aaacgatttg gaactgggta aagcctacgc ttctttgtgc gacgtgttcg
     7261 tcaatgacgc gttcggaacc gcccaccgcg cccaagcctc gaccgaagcc gtcgcccaag
     7321 ccgcgcccgt tgcctgcgcc ggcgtattga tggcgggcga actcgacgct ttgggcaaag
     7381 ccttgaaaca gcccgcgcgc ccgatggttg ccatcgttgc cggcagcaaa gtgtccacca
     7441 agctgaccat cctcgaatcg ctggcggaca aagtcgacca actcatcgtc ggcggcggta
     7501 tcgcaaacac cttcctgttg gcggaaggca aagccatcgg caaatctttg gcggaacacg
     7561 atttggtgga agaatccaaa aaaattatgg cgaaaatggc ggcaaaaggc ggttctgtgc
     7621 cgctgccgac cgatgtcgtc gttgccaaag cctttgccgc cgatgcggaa gctgtggtga
     7681 aagacattgc cgacgttgcc gaagacgaga tgattttgga catcggtccg aaatccgctg
     7741 ccgcgcttgc cgatttgctt aaagccgccg gtacggtcgt ttggaacggg ccggtcggcg
     7801 tgttcgagtt tgaccaattc gcaggcggta cgaaagccct tgccgaagcc attgcccaaa
     7861 gcaaagcgtt ctcgattgcg ggcggcggcg acacgctggc ggcgattgcc aaattcggcg
     7921 ttaccgaaca aatcggctac atctccaccg gcggcggcgc gttccttgag ttcttggaag
     7981 gcaaagagct gcctgccgta gccgctttgg aaaaacgcgg cgcgtaagcg gtttgatata
     8041 aaaaaaatgc cgtctgaaac gaaatggttt cagacggcat ttttgtgtgg cgattattgt
     8101 gctttggctg cccattccgc cggagtggcg gcaaccgaaa tggaaagtgc gtgcagttcg
     8161 ttactttcca gttgggcttt gagtccgtct ttaatcaggc ggtggcgcgc gaggcgtgcc
     8221 ttgccttcaa attctgatga aacgatgacg gcgaaaaaat ggtgtccgtc gccttctact
     8281 tcgatatgtt cgcattttgc cacgccttca atcatggctt ttacttgttc cgaggtcagc
     8341 atggtaggtt ccttgttgga aaaatgcgcc attatactgg caaattgaaa tgccgtctga
     8401 aaacgggttc ggacggcatt tttgacggtg ttttattttt tggctttgaa gccgagcagg
     8461 tgctgggtgg tggcaagcca cgcgccgaat acgcccaacg cgatgacgaa gccgaacacc
     8521 agcccgagtt cgccagcgta gaagaaccgc cagccgatat taagtccgta gggtttgaaa
     8581 atggcatcga ccaatgggcg cacggcagag agcagccaac cgcaaagccc caagctgacg
     8641 gcggcggaaa ggatgctctg ccacatggct tgataaagga atgggcggcg gataaacgac
     8701 gcgggcgcgc ccaagagttt ggtgatttcg atttcttctt tgcggctgag gatttgcagg
     8761 cggatggtgt tgtgtgcgac aaggacgaac gccatcccca gcgtcaggga aagaaaccac
     8821 aaaattttgc ggatgaactc gttgatttgg tacagcgttt gcacccattc ggtatccata
     8881 gacgcggatt cgaccatagg cagtttggta atgtctcggt agattgcctg catttgggcg
     8941 ggcgtggttg ccgggtcggg ggtaacgata aagacatccg gcagggggtt gccgtcaagc
     9001 atggaaatca gattttggtc gagattggac tgtaattctt ccagaccgtc ttctttgccg
     9061 atgaagcgga tgttgtcgag ccgtttgtcg cgcgccagca ggctgcggac ggtatcgctg
     9121 tcgctttgtg cggcggaggt ttccatatag attgtgattt gcggcgactc gttgagtttg
     9181 cccaacacgc tttgcccgct ttggatgccc agatgcataa acagcggcag ggtcatcgcg
     9241 acggcgagca tcatgagggt aagcagtgtg ccgaagggtt ggcgcaggag ctgcttgagc
     9301 gcggtgcgcg cggattcgac gtgcagcgag aggtagtgga tgatgctcat gcgagtcgtc
     9361 ctttcgagag gcgcaggatg cggtgtccgt agtccgccat cagggtttcg tcatgtgcgg
     9421 caacgatgac ggtagttccc gcttcgtgga aggttttgaa caattccata atatcgagcg
     9481 cgtaggcgcg gtcgaggttg gcggagggtt cgtcggcaat cagcaggccg ggctggtgaa
     9541 cgacggcgcg ggcgatgcac aggcgttgtt gttcaccgcc ggagagggtt acgggatcgt
     9601 ccaattctcg tcctttcagg ccgacttttt cgatggcgat gcgggcacgc tcttcggctt
     9661 tgcgcggcgg atagccgata atccgaagcg gcaggatgac gttttgcagg acgttgcggt
     9721 cgtagaggat tttgtggtct tggaacacga tgccgatgtg ttggcgcata aagccgattt
     9781 ggttgtcgga caatgtgccg aggtcctgcc cgttaaacag gattttgccc ctgctcggct
     9841 tggtaatgcc cgaaatcagt ttgaggatgg tggatttgcc cgaaccggag tgtcccgcga
     9901 taaatatcat ttcgcctttg ttgatttgga agctgacgtt tttcagggct tcaaaaccgc
     9961 cgggataggt tttggaaact tgttcgaaac ggatcatagg aggtcctgta aggaagggcg
    10021 gcatatcgtt tcgggcggtt gcgtccgcag ggatatgccg tctgaacaat aggtaagaca
    10081 gtgccgatta taaagattcc gcctgttttt agatagtgga taagtttgca ccggcaggcg
    10141 ggtggaaaag aaaaatgcca tctgaagcgg cttcagacgg catccggcgt ttaacggcat
    10201 tttgaatggg cgagtttgac ggcgtcggtc aggctttttt cgtttacctc cccggtaatg
    10261 gtctgcctgt atccgcattt cggtgcttcg acgacggtaa agggcagtac gccgacagtg
    10321 tttccgtagg ttttcataaa gtttcggctg ttcgccccgg tgtaacgcca aatcgggtag
    10381 gaaacaggag tttgtttgag gaagttgccg atattgtcgg atgtgtcgag cgcgatgccg
    10441 accatatcga cgctgccttt tttctgcgct ttgtaccatt tggacatggc aggcatctct
    10501 tttcggcacg ggccgcacca agtcgcccaa aggttgacga tgcgtacggg ggctttgagc
    10561 gattgcaggc tttgcggggt gttgtctttc catccggcca gttcgtctgc cgaggcggta
    10621 tgtgcggcaa atgtggcggc aagggtgatt gcggcaagat tcaagtattt catcgttttg
    10681 tcctttgtat gtcgattcgg gtattgtaaa ccgtttggta cggttgtagg cgggatattg
    10741 agtaaaaccc tgtgtaagcc gataataagg ggattgtcga gaagcaagga aatgtcatgc
    10801 ctgaaatcaa aattttccac aatccgcgtt gcagcaaatc gcgtgccgcc ttgtccctat
    10861 tggaagaacg cggcattgct gccgaagtgg tcaaatattt ggatacgccg cccgacttgt
    10921 ccgaattgaa ggatattttc aacaaattgg gcttggcatc ggcgcgcggg atgatgcgcg
    10981 tgaaagatga tttgtataag gaattgggtt tggacaaccc caatttggac aatgacgcgc
    11041 tgctgcgtgc catcgccgat catcccgccc tgttggagcg tccgattgtt ttggcaaacg
    11101 gtaaggcggc tgtcggcaga ccgctggaaa atatcgaagc ggtattgtga acaaacggct
    11161 gttttgcagc cgcaacggct tgcgctatta cctgttgggc ggtttctgtt tgagtgtttt
    11221 tccgcttttg ctggtgtttg cctcatccgt ttgggcggtt tatcggacgg gtgggcaggt
    11281 tttgccgccg tatgtccggg cggatgccgc gctggtgttg ggcgccgccg cgtgggacaa
    11341 acgcccttct cctgttttcc gcgaacgcat caaccatgcc atcgcacttt atcagagccg
    11401 cagggtaggc aaaatcgtgt ttacgggagg ccggaccaaa aaaggctata tgaccgaagc
    11461 cgaggtcggg cggcggtacg cactcaaaca gggggttccg gcgcgcaaca tcctgtttga
    11521 aaacacttcg cgcaacactt atgaaaacct gaacaacatc cgtccggttt tgcgtgccaa
    11581 cggtattgcc agcgtggtga ttgtcagcga tccctaccat ttggcgcggg cggcggaaat
    11641 ggcggaggat ttgggtgtcc gcgtttatat gtctgccacg ccgaccacgc ggtttgatgc
    11701 gggaaataag aaaaaaatct ttatgctgca agagggatat gcgttatccc tctaccgttt
    11761 ggaaaaatgg gggcgggatt ttttcaatta gctgtttcat tgaacttttt tttggacgca
    11821 atcccgaagg aattcggtcg gcggcgcgtg atacactgtt ccgcgtgccg aagcattgaa
    11881 aacaaggaaa atgccgtctg aaactttatc cgcaggcagg gttcagacgg catttttgat
    11941 gcccaagcac ggtaaaacca gtaaaataaa cgctttcaaa gatttcggag ccatcatgac
    12001 cgtcaaaacc cgtttcgccc ccagccccac cggctacctg cacatcggcg gcgtgcgcac
    12061 cgccttgttt tcatgggcgt ttgcccgcca tcataaaggc gagttcctat tacgtattga
    12121 agacaccgac ttggcgcgtt ctaccgccga atccgtcaac atcatcctcg acggcatgaa
    12181 atgggtcggt ctcaattacg acaacgccga caacgtcgtt taccaaactc gccgtttcga
    12241 ccgctacaaa gaggtcattg ccgaactttt agaaaaaggc catgcctact actgctattg
    12301 cagcaaagaa gagctggaag ccatgcgtga gaaagccgaa aaagaaggct cggcgactta
    12361 cgaccgccgc tggcgtccgg aagtaggtaa aaccctgccc gaaatcccgt ccgacgtaca
    12421 acccgttgtc cgtttcaaaa cgcctttgga cggtgtgacc aaatggacag acttggtcaa
    12481 aggcgaaatc tccatcccca acgaagcact cgatgacctg attatcgccc gcgccgacgg
    12541 tacgccgacc tacaacttct gcgttgtcgt agacgactac gacatgggcg ttacccacgt
    12601 tatccgcggc gacgaccatg tgaacaacac gccgaaacaa atcaacatct taaaagccat
    12661 cgacgcgaac ctgcccgaat acggccacct gccgatgatt cttaacgaac aaggcaaaaa
    12721 aatctccaaa cgcagcggcg ataccgttgc cattaccgat ttcggcgcaa tgggcatcct
    12781 gcccgaagcg atgctcaact atctggcacg cttgggctgg gcgcacggcg acgatgaatt
    12841 cttcacaatg gaacaattca tcgaatggtt tgatttgaaa gacgtttccc cgtctccaag
    12901 ccgtatggac ttgaaaaaac tctactggat caacggcgaa cacatcaaaa tcacacccaa
    12961 cggcaaactc gccgaactcg tcaaaccccg ccttgcgttg cgcgatattc atgaaaccga
    13021 aaaacctgct ttggaagatg tgttggaact ggtcaaagac cgcccccaag acttgaacac
    13081 gcttgccgac gagtgcttct acttctacgt caaacaaacg cctgccgaag cagacgtgca
    13141 gaaacactgg gacgatgaag ccgccgcccg tatgctgcgc tttgccgaac gcctcgaagg
    13201 gctggaagac tggaatgccg aagccatcca cgacctcttc aaacccttct gcgacgaaga
    13261 aggcattaaa atgggcaaac tcggcatgcc cctgcgcctt gccgtctgcg gtacggcgaa
    13321 aacccccagc gtcgatgctg tgttggcatt aatcggcaaa gaagaagtgt tgaaacgcat
    13381 ccgcgcttaa gttatccaca ggcgtgggcg atgccgtctg atgtcggaat acggcttcag
    13441 acagcattta aatggaagtt aaaaatgaaa aaaatatttt attttctgat ggttgttttt
    13501 tctacaagcg tatgggcagg ggatgctgaa gacaatctgc tcagcatcca atccggttac
    13561 cgcgccttat tgcaaaagca aaacaatctg gacggaaaaa tcatcgggat gcagtcggat
    13621 ttggaagatg cgcgccggcg tttgcagacg gctcaggcag acatcgcccg tttggaagcg
    13681 gaaattcctg cagcaatggc gcaaaaagcc cggcaggctg aagatttgag gcaaatcgga
    13741 gtgcgtttgg accatgcttg gaatgcggtt tacggcgcag ggggaacgaa ggcgtcgggg
    13801 aattgaccga tgaattccct ctttgtggac aatactgttt tcattacacg gctgaaagcc
    13861 gggcatatcg gcaggttggt tcaggcgttg tttgaggagt ggcacggatt tgaaccgtgg
    13921 tcttctgtgg ataagattca tgcctattac ggcaggtgtt tgaaggatga cgaactgccg
    13981 ctggcatttg cggctgtgga tgattccgga atcctgttgg gttcggctgc ggtcaagcgg
    14041 catgatatgg aaagttttcc acggtatgaa tattggttgg gggatgtctt tgttttacct
    14101 gaatatcgcg gaaaaggcat tggcaggagg ctggtcgccc actgcatagg cgcagcgcgt
    14161 tcgctgggga taaagttctt gtatctttat acgtctgatg tgcaaatatt ttatgaatca
    14221 ttcggctggg tggttgtcgg gcgacatttc cataacggtg aatgggttac ggttatgcgt
    14281 ttggatgtgg ataaggttta agccgaatgg acggatgccg tctgagctgt tcggacggca
    14341 ttttttatgt ttggaaattt cctgtcagat aatgtttatc cacagatttt tccatatggt
    14401 ttgatgctgt tccgacaacg gaaattaaga tacggatttg cacaaatctt tcagacggta
    14461 cagttctgac aatgcttcgc gcggggtcag gctgtcggga tcgagttttt ccaaggctgc
    14521 tgccaatata ccttcaaaat gtttttcctc tgctttatcc acaaagttgc ccacattcgg
    14581 ttcatctcct ttttcagacg gcatggtact gaaaatatcc agttggggac ggttcgcggc
    14641 ggcttggttt tccagtccgt tcaaatgctt ttgggcggat ttcaatgcgc gtacaggcag
    14701 gccggcgagt ttggcgacgg caatgccgta gcttttaccg gcgggacccg gttggatttg
    14761 gtgcaggaaa acgatgtcct gtccctgttc gagcgcggaa aggtgcatat tgacggcggc
    14821 ggtgtgggct tcgggcaggt aggtcagctc gaaatagtgg gtagcaaaca ggctgaagga
    14881 tttgtttttt tgcagcaggt gttcggcaac ggcgtgcgcg agggcgaggc cgtcgaaagt
    14941 ggaagtacca cgtccgactt cgtccattaa aacaaggctt tgttcggtgg cgtgatgcag
    15001 gatgtaggcg gtttcgctca tttcgaccat gaaagtggag cggttggagg cgaggtcgtc
    15061 cgatgcgccg atgcgggtga agatttgatc gatgggcccg attgtggcgg catcggcagg
    15121 cacaaaacag ccggtgtgtg ccaataaaac aatcagcgcg acttggcgca tgtaggtgga
    15181 tttgccgccc atattggggc cggtgagcag catgaggcgg tgtttgtggt caaggtcggt
    15241 gtggttggcg gtgaagtggc gtacctgctg ttcgacaacg ggatggcggc cgttttcgat
    15301 gtggataacc ggatagtctg caaactcggg gcggacgaag ttccgctctt ttgccaaggc
    15361 tgaaaatgtg gacaacacgt ccagcgcggc ggcggctttg gcggcttttt gaagctgcgg
    15421 caatgccgtc tgaaggtttt tcaatacgcc gtcaaagagt tgtttttcta aggcgagggc
    15481 ttgctcttga gcagtcagca ctttgtcttc aaaggctttc agttccggcg tgatgaagcg
    15541 ttcggcgttt ttaagggttt gccggcgttg gtagtcggca ggtgcttgtt cggcttgggt
    15601 tttggacaat tcaatgtaaa agccgtgaac gcggttgaac tcgactttaa gtgtggacaa
    15661 accggtacgt tcgcgttcct tggcttccaa atccagcaaa aattcgtcgc catggttttg
    15721 aatgcggcgc aattcgtcca gttcgggatg aaaaccgtgg ttgatgacat tgccgtcttt
    15781 cagccagacg gaaggttcgg gcaaaatggc ttggcggagc tgttcggctg tggataggtt
    15841 ttccgggaaa acggctttga gggttcctaa gagactgctg cactcggcgg acaattcgat
    15901 ttcggacagg gcaaacaggc tgtcgcgcag ggcggcgagg tcgcgcgggc gggcgttacc
    15961 cacggcaata cgggcggcga tgcgttcgat gtcggcaatg cttttcagac ggcactggag
    16021 gggtttgtat tggctttcca gcgcggcaac ggcttcttgg cgcgctcgga tgtgggcgcg
    16081 gttgcgtaaa gggtggtgca gccagagagc caagaggcgg ctgcccatat gggtagcgca
    16141 aaggtcgagc gtggacatca gggtcggcga ttttttgccg gagagggttt gcgtgatttc
    16201 gagattgcgg cgcgtggcgg catccatacc gatatattgg ctgtcggttt cgagcgacag
    16261 gccgtccaaa tgttgcggca tcaggttttg cgtcagacgg atatagttca acagtgcacc
    16321 tgccgcgcca atcgcaacgg cgtgttcttt gccgtccaaa ccgaagccgc gcaaatcctg
    16381 gcagccgaaa tattccgtca gcagtttttc ccccgcgtcg gcggcaaact gccacgcgtt
    16441 caggcgcgta acacccgatg ccgtctgaag ttgcggtgcg tttttactgt caggcaacag
    16501 aatttccgcc gcctgcaggc gcgccagttc gtcgtccaat ttatccacag ttgtcagctt
    16561 ggttttgaat tcgccgcttt gcagcgatgc ccacgccaaa ccgatgtatt ttttgtcggg
    16621 ggacacggca acgatgcggt tggtttcctt gtcttccagc aatgcggaat cggtcagcgt
    16681 gccgggcgtt acgatgcgca cgactttgcg ctccacaggc cctttgcccg cgccgacttc
    16741 gccgacctgt tcgcaaatcg ccacgctttt gcccaacttg accaggcgcg ccagatattg
    16801 ttcggcggcg tgaaacggca cgcctgccat tttgaccggc tcgccatcca cctgtccgcg
    16861 cgtggtcagg gtaatatcca aaagttttgc cgcttctacc gcatcgtcga aaaacatctc
    16921 gtaaaaatcg cccatacggt aaaacaccag tttgtcggta tgttgcgctt tgatgccgag
    16981 gtattgctgc atcattgggg aaacggcgga tttggacatg gggctgcctt atcattagga
    17041 aaacggtatt gtagcaaaag gttttgtctt cgacggaatg agactgaccg taagcctgtc
    17101 aataaggttg tggagacaac taggaaaaca gcaaagttat ccacagaaat attattaagt
    17161 caaaaaacat ccacgcttat ctgtcttata cagcaagtct taaactcaaa cttatttgac
    17221 aaaaaggtaa tgaaaatatt ggatatttgc tacttacgca caagcaggtt ggcaatcatc
    17281 aaaatcatca ttaaaatttt aaattcaaat attgatggca aaagttttgg aaatctgtgc
    17341 aaacagtaaa attcatctat atcaaataag cggcaagtag ttagatgtaa tagtggaatg
    17401 taactttatt tcagaataca atacaggtat tgaaatttcg tttcacagct tggagtatat
    17461 acatgagcat taaagtagcg attaacggtt tcggccgcat cggccgcctc gcattgcgtc
    17521 aaattgaaaa agcccacgac atcgaagtcg tcgccgtcaa cgacctgacc cctgccgaaa
    17581 tgctgctgca ccttttcaaa tacgacagca cacaaggccg tttccaaggt actgccgaat
    17641 taaaagacga tgccatcgtg gtaaacggca aagagatcaa agtttttgcc aatccgaatc
    17701 ccgaagaatt gccttggggc gagttgggtg tggatgtcgt cctcgaatgt accggcttct
    17761 ttaccaataa aaccaaagcc gaagcacaca tcagggccgg cgcgcgcaaa gtcgtgattt
    17821 ccgcacccgg cggcaatgat gtgaaaaccg tcgtatatgg cgtaaaccaa gacattttgg
    17881 acggcagcga aaccgttatc tccgccgctt cctgcaccac caactgcctt gccccgatgg
    17941 cggcagtcct gcaaaaagag ttcggcgtag tcgaaggtct gatgaccacc atccacgcct
    18001 acaccggcga ccaaaacacc cttgacgcgc cgcaccgcaa aggcgatttg cgccgcgccc
    18061 gagccgccgc gctcaacatc gtgcccaaca gcaccggtgc cgccaaggcc atcggtctgg
    18121 ttatccccga attgaacggc aaactcgacg gctccgccca acgcgtccct gttgccagcg
    18181 gctcgctgac cgaattggtt tccatcctcg aacgccctgt aaccaaagaa gaaatcaacg
    18241 ccgcgatgaa agccgccgcc agcgaatctt acggctacaa cgaagatcaa atcgtttctt
    18301 ccgacgttgt cggcatcgaa tacggctcgc tcttcgatgc gacccaaacc cgcgtgatga
    18361 ccgtgggcgg caaacaattg gtgaaaaccg ttgcctggta cgacaacgaa atgtcctaca
    18421 cctgccaact cgtccgcact ttggaatact tcgcaggcaa aatctaaggc ttgaacaaac
    18481 ctgtggataa gtaaatgccg tctgaacaca ttgcgttcag acggcattgt ttatctgtat
    18541 agtggattaa atttaaacca gtacggcgtt atctcgcctt gccgtactat ttgtactgtc
    18601 tgcggcttcg ttgccttgtc ctgatttaaa tttaatccgc tataacttga tattgcggct
    18661 tatggcgaac aaacaacagc cgtttgttca actgctatgc ggcaaaaccg gatttttgcc
    18721 cgatttaaag cggggttggg gaaagatcaa taacggttgc acggttttcc tcgatttgaa
    18781 atctgacttc ataatctgca atattcatca cataaatccg ttcgggaata ttctgatagg
    18841 cggggcgcgg atcttgggca atgctttggc tgataaggtt tttggtgttt gcagataaat
    18901 tttccgcgcc gatgttttcc tgccaaacga cttccaactc tacgggtttg ccgctgacga
    18961 aaccggatgc ggcatcgggt ttggattcga caaaggggat ataaggtttg atgtccacaa
    19021 tcggtgtgcc gtccagcagg tctgcgccgc tgcaatagag gcggacgggt ttgccggttt
    19081 cgatgcgttc gagtttcagg agcgagagtc cgagatggtt ggggcggtgg gggctgcgcg
    19141 tggcgaacac gcccattttt tgtttgccgc cgagccgtgg cgggcgcacc atttgcgccc
    19201 agccttcatc caatacgccg tgaaaaataa aacttatcca cacataatcg aaatcttcca
    19261 gcccgcgcac gctgtctgcg gtgaatttgg gattcagctc gatgcaggct tttgcggcgg
    19321 agaccaaacc gggctggcgg gcgatgccga atttctgttt gtagggcgag cgggcggtgc
    19381 cgatgggggt aatggtataa gtcataaggt tgtggataag ttttttggaa aaaccttatc
    19441 ttatcacaat cggttttacc gcttttgggc agtaatttgt tataatacag ggcattttaa
    19501 tcgcgttgaa ataatgagat gattgtttcc attgatgttg atgcacaaaa aacgtttacg
    19561 ccactgtgtc ctgacgaact gcccgtgaac gaggggcatt tgattgtcga ggagttgaat
    19621 gcgcaagccg ctttggcgga tttgcgcgtg atgacgaaag atgcgcatca tatggtagcg
    19681 aaatggcttg tggataaccc tgttgatatg ttgaagccga caggtttgcc tgatgcggat
    19741 ttgacttggg tggctcatgc gatggtcggt acgcgcggct atgaattatt ggacgggctg
    19801 ccttctgcta aagaatacga ttattgcgtt tggaaaggtg ttgatcctga attgcatcct
    19861 tacggcgcgt gttttcacga tattgaggaa aaactaagca cagggctgat tgaatggctg
    19921 cgttgtcaaa atacggatac ggttattatc ggtgggttag ctacggatta ttgtgttaaa
    19981 acaacggttt tgcagttact caaaggcggt cgttggcagg ttgtcgtcaa tgaagcggct
    20041 tgtcggggta ttgcgccgga aaccatcgaa acagcatggc aggaaatgca ttctgctggg
    20101 gcaatcatct taaaaaacgc tgatgaaata aataaatata ttaataatca ataagttaaa
    20161 attgtttcac gtgaaacccc cttcctaaat atgaaaattt tgcttgtccg cttgtctagt
    20221 atgggcgatt taattcacac tttgcccgca atcgaagatt tggcgcgaca atgtcctgat
    20281 gtagaactgc attggctatg tgaggctgga tttgcagata ttgcgcgcct gcatccgttt
    20341 gtaaaaaaaa tccatgtgat gaaatggcgg caatggcgca aacatctctt tcgggctgaa
    20401 acttggcggg aaatgggtca tctgaaacag actttgcggc aggaagtatt tgatttcgta
    20461 ttggacagtc aaggtctgat taaaagcgcg tgtttcgcta aaatggcaaa atccccgatt
    20521 tgtggtttgg ataaaaacag tgcgcgcgag ggatgggctg ctttggcgta tgtaaaaaca
    20581 tacgctgtac cgaagggaaa aaatgccgtt tggcgcaacc gtgaactgtt tgcccaagta
    20641 tttgggtatg taatgccgga aacgcaggta tttggcttga ctgttcctga agcaggccgt
    20701 ctgaaaaatt tagcgcagcc gtattatgcg gctttgcatg cgaccagtcg ggacagtaag
    20761 ttatggcctg tggaaaactg gcggtcgttg ctgcaaaagt tgaatgaaga acagcaatgc
    20821 aatatttacc tgccttgggg aaatgaagat gaaaaaacgc gtgccaaaca aattgcagat
    20881 gggctgccgt ttgctattgt gtgcgacaaa ataaatttat tgcaggcagc gtatctgctg
    20941 aaatacgcgg tcggaattgt cggcgtggat accggtttgc tgcatttggc aaatgccttg
    21001 gaaaaacctg tggtcggcat ttataccgat accgatccga ttaaaacagg cgttcaggtt
    21061 tcgccaattg caaaaaattt gggcaatatc gggcagattc cgaccgcaga tttggtttat
    21121 caagcgctga tggattgtgt tgcagcagat aaaggctaaa gggtcgtcta aaactgatat
    21181 ggcggcatga tattttccaa tttattagat acaggaatgt atatccgcta gtctgagtaa
    21241 atgctttaaa aatggtataa tcggcaagca ttatccgaaa gatttttgaa tgaattcgtt
    21301 tttaaggaaa acggatttgg ttttataggg aagaaggttt ggtttcgctg gatggtgaaa
    21361 gcagcgttct ttttaggagg gttcaatgaa agcactggtc gcagtaaagc gcgtagtgga
    21421 ctacaacgtc aaagttcgtg taaaagccga tggttcggat gtggatatcg gcaatgtcaa
    21481 aatgtcgatg aatccgtttg acgaaatcgc tgtggaagaa gctgtccgtt tgaaagaagc
    21541 cggaaaagta agcgaaatcg tagcggtttc tttgggtgag aaaaaatgcg aagaaacctt
    21601 gcgtacagct ttggcgatgg gtgccgaccg tgccattcat gttgaaaccg atactaaact
    21661 ggagtctctg gccgttgcta agttgctgaa agccgttgcg gacaaagaaa atccgcaaat
    21721 tttctttttg ggtaaacaag cgattgatga tgatgccaat caagtggcgc aaatgctggc
    21781 agctttgctg aatgcggcgc aaggtacgtt cgcgtccaaa gtacaaattg aaggcgacga
    21841 agtgcagatt gtgcgggaaa tcgatggcgg cgaagaaacc atcgcattga aactgcctgc
    21901 tgttatcagc gcggatttgc gtttgaacga gccgcgcttt gtcaaactcc ccaatattat
    21961 ggcggcaaag aaaaaacctt tggaaaaact gactcctgac gatttggttg ccgacatttc
    22021 acctcgtttg aaaacggtga aatttgccga acctaaggcg cgtcaggcag gcgtaaaagt
    22081 agcaagcgtt gccgaattgg ttgaaaaatt gaaaaacgaa gccaaagtga tttgaggagg
    22141 ctgaaatgag tgtattgatt attgtcgaac acgacaacaa acagttgaat cctaccactt
    22201 tgcatgctgt taccgctgcc gccaaactgg gcaaagtcga tttattggtt gccggaaacg
    22261 gcgcatctgc tgtagtggaa ttcgcgaagc aagtagcggg ggtgaaaaaa gttttggttg
    22321 cagatgctgc tcattatgcc gaaggtttgg ctgaagagtt ggctccgctg gttgttaaat
    22381 tggcggccga ttaccgctat gttgcagcaa cggcaaccac attcggtaaa aaccttttgc
    22441 cccgcgtagc agccttatta gacgtaccgc aaatttctga tttgaccgaa attgtggata
    22501 acacgacttt tgtgcgcccc atttatgcgg gtaatgcatt tgaaaccgtg caagctgatt
    22561 cagaaaaatt ggtgctgacc ttccgtgcga cggtttttga cgcagtggcg gcgcaaggtg
    22621 gtaatgctga ggtaattaat gttgaagcaa cccctgccca aaacctgagt cgttttgtta
    22681 accgtcagct ttcccattcc gatcgtcctg aactgactca ggcaaaagtg attgtttccg
    22741 gtggtcgtgc gttgggtagt gcggaaaaat tcaatgaagt gctgacaccg ttggcggatg
    22801 ttttaggtgc ggcaatcggt gcatcccgtg cagcagttga tgccgaatat gcgccaaacg
    22861 atgctcaagt cggacaaacc ggtaaagtgg ttgcgccgca actctatttt gcaatcggta
    22921 tttcaggtgc aattcaacac gttgccggta tgcaagacag taaggtgatt gttgcaatca
    22981 ataaagatgc tgatgcgccg attttcaatg tagccgatta cggattggtt ggcgatttat
    23041 ttgaaatcgt tccacaatta acagaagtat tgaaaaattg atgtttcacg tgaaactttc
    23101 agacgacctt gttattaatg aggtcgtctg aaaacatttc agcatgatta cgacttataa
    23161 aaccattact tcctcgacgc aggctgagtt taaagataaa ggcagccgtt ttattgcatt
    23221 tgcctatccg attcggacat tggctgatgt gaaaaaatac ctcgatccgt taaaggaaga
    23281 gcatcataaa gcacgacact ggtgctatgc ctatcgtttg ggtgtggatg gtgtgcaatt
    23341 tcgtgccaac gatgatggag agccatcagg aagtgctgga cgaccaattt tgggacagat
    23401 tgattcggtg ggtattaccg acgttttggt tgtggtcgtc cgctatttcg gtggtacttt
    23461 gttaggtgtt ccaggtctga tacatgcgta caaagaggcg acggctcaag cgttggcagt
    23521 tgcagaagta gttgaaaaga atattgaaaa aactgtttgg ctgaaatgtg aatatccgtt
    23581 tttgaatgaa gcgatacgca ttgccaaaca atatcaggcg gatatattgg agcgggattt
    23641 gcagttggat tgccgattga cggtcagttt gtcgttggta aattatgagg cctgtgttgc
    23701 ggcttggaag aatactcggc agattgaagt aaatacggaa aagccttttg aatgaaaggt
    23761 cgtctgaaat gctttgtgat ggtatttcag acgacctctt cgctgactaa tcttcttgtg
    23821 tatatttgtc taaggcatcg acgctggagc gcaaatataa ccaagccaat tgatggaatt
    23881 cgcccagcgc gttccatagc gcatcttcac tcataatgct gtattcgcct tccgtactca
    23941 aatccagttc taccccgtct tcaatagcct gatgacacgc ctgatactcg tgggtataga
    24001 aattgtccat gtcgcgaatt aaagtgacga catgtttcca acggcggatg tcttcttcca
    24061 gttgcggtag taaatgacac cattcgcggt atttgctttg gatttcatca atacgttttt
    24121 gcatggttgc ttgtcctttt ttaatttatg gataagttga ataggttgca gagtggaatg
    24181 aagcggtctt ttgtttgatt ctactaaaat ttcttcagat gggataaaag tccctttgta
    24241 tcgagttttc gttaaaagta catatttgtc aaggagatgg tatgaaactg ctggttatcg
    24301 gtaatggcgg tcgcgaacac gcgctggctt ggaaattggc gcagtcgcct aaagtggaaa
    24361 cagtatttgt tgcgcctggt aatgccggta cagcgattga acccaagttg caaaacatcg
    24421 acttgactgc gcatcaggat ttgattgaat tctgtcgtaa agaaaatatt gtttttaccg
    24481 tcgtcggccc tgaagcgcct ttggcggcag gtattgtgga tgatttccgt gctgcaggac
    24541 taaaaatatt cggtccgaca caatatgcgg cgcagttgga aagttccaaa gatttcgcca
    24601 aagcatttat ggcgaaatac aatattccga ccgcgcaata tcaaaccttt gaaaacgccg
    24661 atgccgcaca tgattacgtc aatcagaaag gtgcgcctat cgttatcaaa gccgatggtt
    24721 tggcggcagg taaaggcgtg attgtggcga tgactttgga tgaagcgcat gctgcgattg
    24781 atgatatgct gctggacaac aaaatgggca atgccggcgc gcgtgttgta attgaagatt
    24841 tcctgcaagg cgaagaagcg agttttatcg tcatggttga tggcaataat gtactgccta
    24901 tggcaaccag ccaagaccac aagcgtcttt tagacggcga caaaggtctg aatacgggcg
    24961 gtatgggtgc gtacagccct gcgcctgtgg taacgcctgt tgtgtacgaa cgcgcgatga
    25021 atgaaattat tttgccgacc gtagcaggga tgaaagcgga ggggcatgag ttcaccggtt
    25081 tcctgtacgc aggtttgatg attgatcaaa gcggtgcacc ctatacgatt gagtttaact
    25141 gccgtttcgg cgatcctgaa acccagccga ttatgagccg actgaacagc gacttatcgg
    25201 atttggttga agcggcaata gacggcaaac tcgatagcgt aaccgcggaa tggagcccgc
    25261 aaactgccgt gggcgtggta ctggcggcgc aaaattaccc cgaaacgccc aaaaaaggcg
    25321 atattatttc cggtctggat gctgccaacc aaatcggcaa agttttccat gcaggtacaa
    25381 cggcaaacga gaaaggtgac gtattgacca acggcggacg cgtattgtgt gttgtgggat
    25441 tgggcgataa tgtggcgcaa gcgaaagcta aggcatatgg cgcgttggaa aaaatcagtt
    25501 tcgacgggat gcaataccgt aaagacattg ccgataaagc gattaaccgt taattagaaa
    25561 gaggatgatg ccgtctgaag ccaagtatga ctaatgtgct tcagacggta ttttatatta
    25621 tgttatttcc gagaattatc gcagcatcag cgcaacgtaa gttaagcgtt tatttgaaaa
    25681 aaggtggttt ggtcgcttat ccgaccgaat cttgttatgg cttggggtgt cttccgacat
    25741 tagccaaagc actcggtaag ttggcgcatt tgaaaaaaag accgcaacac aagggtatga
    25801 ttgtcatcgg gaatcaattt gaacaattgc agcctttact tcaaatgccg tctgaaaatc
    25861 ttcaggatat gcttagaaaa gaatggcccg cccctaaaac gttcctatta tccgccaaat
    25921 cctgtgtctt acccgagtta cgcgggaaac agcgcagcaa gttggcggtc agggttcccg
    25981 cccatgtagg tgcgcgccgc ttgtgtcagg ctttgcaaac gcctttggtc tcgacttcgt
    26041 gcaaccgcgc aggcaaacgc gcgtgtcgga cggagaggga agtcagaagg caattcggac
    26101 gcgatgtgtg gattgttggt gggagaatcg gcaggcagaa atcgccgagt cagattattg
    26161 acggggaaac aggtaaacgt ttgcgttaga tatgggtact tgaatgaaaa ctttttttgc
    26221 atacacaaag aaaaaatttt tcggatagca tttctctaca tgattgttat tgcacagaga
    26281 taaaggtaga aggacgcaac gtctgcttta attttgagga cggttttgtc gttccggaca
    26341 ataatccgaa caatcaagcg ggcaagcatc tgaaaacaga tttttcccgt gttgttttaa
    26401 tccataaaaa tgaaaacgcc gaagatgatt a
//
LOCUS       DAWIVB010000008        25462 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWIVB010000008 DAWIVB010000000
VERSION     DAWIVB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.008"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..347)
                     /locus_tag="WIX60_000242"
     CDS             complement(<1..347)
                     /locus_tag="WIX60_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEZ5304571.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDG"
     gene            complement(344..1486)
                     /locus_tag="WIX60_000243"
     CDS             complement(344..1486)
                     /locus_tag="WIX60_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ5304572.1"
                     /translation="MGKSLTEIAEELKGNDKKVQLIYAFNGTGKTRLSREFKNLIAPT
                     SSEEPDGEPTRRKFLYYNAFTEDLFFWDNDLLANEAPRLKIQKNSFTDWLLRDNGLDG
                     AVIKNFQYYTDDKLTPDFNDDFSEIAFYFARGNDEQIENIKISKGEESNFIWSIFYVL
                     IRQVIAELNIPEDSEEGRSTDQFDDLEYIFIDDPVSSLDENHLIQQAVDLADLIKLSK
                     PRLKFIITTHNVLFYNVLYNELKKLEKEKKSYLLLKNEDGSFDILEKQGDSNKSFSYH
                     LHLKGVIEKAIENQQVERFHFMLLRNLYEKTANFLGYKQRSDILPEDSRRNYFQRIIN
                     FTSHSTLSNEAFAEPTPQEQETVKLLLQHLLDNYNFFQDDEQRDKP"
     gene            complement(1544..2149)
                     /locus_tag="WIX60_000244"
     CDS             complement(1544..2149)
                     /locus_tag="WIX60_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ5304573.1"
                     /translation="MLTIKSGGIADGYKGRLKDVVWKTLGEVFNIFAGGDVPKDAFSE
                     VETEEFCIPILSNGIGSKALYGWTNVAKINQPSLTISARGTIGWASFQNKPFFPIVRL
                     LVLTPKIELNLKYAYYFMKSIESNYKVPESGIPQLTKPMIKDISIPIPPLPEQEKIVA
                     ILDKFDTLTHSISEGLPYEIALRRKQYEYYRGQLLSFPKAA"
     gene            complement(2217..2705)
                     /locus_tag="WIX60_000245"
     CDS             complement(2217..2705)
                     /locus_tag="WIX60_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ5304574.1"
                     /translation="MDMQNKAKKLIEMIQTAPVEWKPLGEVAKVLRGKRLTKKELIEG
                     GKYPVFHGGLIPLGWFDQFNRRANQTMIINTGSIGEVIWSGVDFWSSDGTFVIQTPNY
                     LDDKFIFYFLKTREGYIKSQKRVGGVPTIDRLVVENISIPIPPLETQQKIVKILDKFT
                     EL"
     gene            complement(2871..3881)
                     /locus_tag="WIX60_000246"
     CDS             complement(2871..3881)
                     /locus_tag="WIX60_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ5304575.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(3878..5422)
                     /locus_tag="WIX60_000247"
     CDS             complement(3878..5422)
                     /locus_tag="WIX60_000247"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ5304576.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFENHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNQF
                     HIELGDTLTNPKLKDSKPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDASGFFKKETNNNVLIEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREIIDIKQLNAEIGETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(5496..6500)
                     /gene="rfaD"
                     /locus_tag="WIX60_000248"
     CDS             complement(5496..6500)
                     /gene="rfaD"
                     /locus_tag="WIX60_000248"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ5304577.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(6629..7660)
                     /locus_tag="WIX60_000249"
     CDS             complement(6629..7660)
                     /locus_tag="WIX60_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ5304578.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFYKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMSQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(7662..8897)
                     /gene="dcm"
                     /locus_tag="WIX60_000250"
     CDS             complement(7662..8897)
                     /gene="dcm"
                     /locus_tag="WIX60_000250"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ5304579.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIYPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGAKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(8934..9905)
                     /gene="hldA"
                     /locus_tag="WIX60_000251"
     CDS             complement(8934..9905)
                     /gene="hldA"
                     /locus_tag="WIX60_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ5304580.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(9960..10700)
                     /gene="pyrF"
                     /locus_tag="WIX60_000252"
     CDS             complement(9960..10700)
                     /gene="pyrF"
                     /locus_tag="WIX60_000252"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ5304581.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(11226..11873)
                     /gene="adk"
                     /locus_tag="WIX60_000253"
     CDS             complement(11226..11873)
                     /gene="adk"
                     /locus_tag="WIX60_000253"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ5304582.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            12089..12928
                     /gene="htpX"
                     /locus_tag="WIX60_000254"
     CDS             12089..12928
                     /gene="htpX"
                     /locus_tag="WIX60_000254"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ5304583.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(13229..13789)
                     /locus_tag="WIX60_000255"
     CDS             complement(13229..13789)
                     /locus_tag="WIX60_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304584.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(13903..14295)
                     /locus_tag="WIX60_000256"
     CDS             complement(13903..14295)
                     /locus_tag="WIX60_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ5304585.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(14503..14895)
                     /locus_tag="WIX60_000257"
     CDS             complement(14503..14895)
                     /locus_tag="WIX60_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ5304586.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(15012..15422)
                     /locus_tag="WIX60_000258"
     CDS             complement(15012..15422)
                     /locus_tag="WIX60_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ5304587.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(15436..15873)
                     /locus_tag="WIX60_000259"
     CDS             complement(15436..15873)
                     /locus_tag="WIX60_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ5304588.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(16284..17582)
                     /locus_tag="WIX60_000260"
     CDS             complement(16284..17582)
                     /locus_tag="WIX60_000260"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ5304589.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGVGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(17686..18837)
                     /locus_tag="WIX60_000261"
     CDS             complement(17686..18837)
                     /locus_tag="WIX60_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ5304590.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            19172..20551
                     /gene="norM"
                     /locus_tag="WIX60_000262"
     CDS             19172..20551
                     /gene="norM"
                     /locus_tag="WIX60_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ5304591.1"
                     /translation="MLLDLNRFSFPVFLKEVRLLTTLALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYT
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            20595..21635
                     /gene="murB"
                     /locus_tag="WIX60_000263"
     CDS             20595..21635
                     /gene="murB"
                     /locus_tag="WIX60_000263"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ5304592.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELRDIVRLLEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVELANADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPTLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            21647..22297
                     /locus_tag="WIX60_000264"
     CDS             21647..22297
                     /locus_tag="WIX60_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ5304593.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            22352..23149
                     /locus_tag="WIX60_000265"
     CDS             22352..23149
                     /locus_tag="WIX60_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ5304594.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAVKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHADKPVWFFLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLPDSGISNI
                     DILRLGGLYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKN
                     IIEPRHPTRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            23200..23766
                     /locus_tag="WIX60_000266"
     CDS             23200..23766
                     /locus_tag="WIX60_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ5304595.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            23786..24676
                     /locus_tag="WIX60_000267"
     CDS             23786..24676
                     /locus_tag="WIX60_000267"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ5304596.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEDCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIAPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            24701..25459
                     /locus_tag="WIX60_000268"
     CDS             24701..25459
                     /locus_tag="WIX60_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ5304597.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
BASE COUNT         6079 a         6502 c         6097 g         6784 t
ORIGIN      
        1 ccgtcatcaa aagcgaaatc gtaaatatgg tcgtcgtgga ttttgcgggt tttatcggta
       61 atgttttcag acggcctgtc cagatattcc gtcaaaaacc gcgcccattc gccgtctgaa
      121 aacgccacat cgttcagccg ctgcaactgc gcgcgcaggt tttccagcag cctgctttgg
      181 ctgttcaaat ccttgcggta ttcgtaaccc tgattctgca aatcggcgat taactccgct
      241 tccaaccggt tttccgattg gtagctgccc gactgttcga ttttttcata ttggtcgagc
      301 acgatgaaat tcggcgtttc agcgatgggt ttggtttcga ggttcatggc ttatctcttt
      361 gttcatcatc ttgaaaaaaa ttatagttat ccagcaagtg ttgcaaaagc aatttgacag
      421 tttcttgttc ttgtggtgtt ggctcggcaa atgcctcatt agataatgta gaatgacttg
      481 taaagttaat aatacgttga aaatagtttc gtctgctgtc ttcgggcaaa atatcagacc
      541 tttgcttata gcctaaaaaa ttagctgttt tttcatacag gtttctcagc aacataaaat
      601 gaaaccgttc tacctgctga ttctcgatag ctttttcaat aactcctttt aagtgaaggt
      661 gatatgaaaa acttttattg gaatcacctt gtttttcaag aatatcaaaa ctaccatctt
      721 cattttttaa cagaagataa cttttctttt ccttttctaa tttttttagt tcattgtata
      781 gaacgttgta aaataaaaca ttatgtgtag taatgataaa ctttaacctc ggtttgctaa
      841 gctttatcaa atcagccaaa tcaaccgctt gctgaatcag atgattttca tccaaagagc
      901 tgacagggtc atcaataaaa atatattcca aatcgtcaaa ctgatctgtg gaacgtcctt
      961 cttcgctatc ttctggaata ttcaattcag cgatgacttg tctgattaat acatagaaaa
     1021 tgctccaaat aaaattactt tcttcacctt tggaaatttt gatattttca atctgctcat
     1081 cattaccacg agcaaaataa aatgcaattt ctgaaaaatc atcattaaaa tcaggagtca
     1141 acttatcatc tgtataatat tgaaagtttt taataacagc tccatccagt ccattatccc
     1201 taagcaacca gtcggtaaaa ctattctttt gaatttttaa tcttggagct tcgttcgcta
     1261 acaaatcatt gtcccaaaag aataaatcct cagtaaatgc attatagtag agaaattttc
     1321 ttcttgttgg ctctccgtct ggctcttctg aactggttgg agcaattaaa ttcttaaact
     1381 ctctggacaa acgtgttttc cctgttccgt taaaagcata gattagctgg acttttttat
     1441 cgtttccttt tagttcctca gcaatttcgg ttaaactttt tcccatgata tttccttatc
     1501 aatatttttc agacggcatg atttaaagac cagctatgac ttttcaggca gcctttggga
     1561 agctcaacaa ctgcccgcgg taatattcgt attgtttccg gcgcagggca atttcgtacg
     1621 gtaggccctc gctgatggag tgggtcaggg tgtcgaattt gtcgaggatg gcgacgattt
     1681 tttcctgttc ggggagtgga gggatgggga ttgaaatatc ttttatcatt ggtttcgtta
     1741 gctgtggaat accgctttca ggaactttat aatttgattc aatacttttc ataaagtagt
     1801 aggcatattt taggtttaat tcaatttttg gtgttaacac taacaggcgt actattggga
     1861 aaaaaggttt attctgaaag ctagcccaac ctatagttcc tctagctgat atagttaagc
     1921 taggttgatt gattttagca acattagtcc agccatatag tgccttactt cctataccat
     1981 tagataaaat ggggatacaa aattcttccg tttccacttc agagaaagcg tcttttggta
     2041 cgtcgcctcc agcaaaaata ttaaatacct cccccaacgt cttccaaacc acatctttca
     2101 gacggccttt atagccatca gctatccccc ccgatttgat tgttaaaatc taaaagaaag
     2161 tcgcggtaat accggtattg gcgtttgcgc agggttaatt ccgcttccag cgtagcttac
     2221 agctctgtga atttgtcaag tatttttaca attttttgtt gggtttccag gggtgggatg
     2281 gggatcgaaa tattttcaac tactaatcta tcaatagtag gaactccacc aactctcttt
     2341 tgggatttta tatatccttc tcttgttttt aaaaagtaga atataaactt atcatcaaga
     2401 tagtttggtg tttgaatcac aaaagtacca tcagatgacc agaaatctac gccactccat
     2461 ataacttcac caatacttcc cgtattaata atcatcgttt gattagctct acgattaaac
     2521 tggtcaaacc aaccaagagg aatcaatccg ccatgaaaaa ccggatattt cccaccttca
     2581 attagctctt tctttgtcaa acgttttcct cttaatactt tcgccacttc ccccaacggc
     2641 ttccactcca ccggtgccgt ctgaatcatc tcaatcaatt ttttcgcttt gttttgcata
     2701 tccatggttg ttttccgttt ctttcccgtt gcggcgggat gtggggcagt tgcggggtgc
     2761 ttcggctcac tctgtccgtt tgccgttatt tctgcgggct gaagtccgtc ctgctttcag
     2821 gctgcctgca atggtgtgca ggctgctttt tcaggtagcc tgaatggtag ttattttgtg
     2881 cggtcttttt tctgtttgat ctgttgcacg gctgtttttt ccaacgcttt aacgctttcc
     2941 agataggctt gctccaccgg cgacaggatg cgcgcttcat aggcgcggta ttcgcgttcg
     3001 gctttctgct ctgcctgttt gcggctgatg ctgcctgcac cctgtaatgt gccttgtccg
     3061 tacagcccgg aaaatttgtc caattcggct atccagtcgc gcatatacat ggggctttgc
     3121 tcctgcgctt tgatttccgc taggtcgaag aaggcggaaa ccagacggtt caggcggaac
     3181 agttcgtctt cggtcagata gtttttggcg attttggctt cattcagcgt ggggattgcg
     3241 ccttgaaagg tggtcagccc cataaagtct ttgctgctgt cggcacggct gtatatcagc
     3301 tcagctgcgg tttgccggct ggcggcatag tgcagtttgt tttgaacggc ggcaaaaaag
     3361 gtttggcttt cgctgctttt ggggttgtag tcttggctgg tggcatataa atcaagcact
     3421 tgccggtata gggctttttc gctgctgcgg atgtcgcgaa tgcggttgag cagttctttc
     3481 caatagtcgc cgccgcctgt gcctttcagg cgttcgtcgt ctatggcaaa gcctttggtc
     3541 agatattcgt ccagccgttc ggttgcccat tggcggaatt ggatgccccg cgcggaacgg
     3601 acgcggtagc cgacggcaat aatcatgggc agggaataat gggcgatttt gcggtttacc
     3661 ttgcgcccgt tttcattttg aactgtcaac tggaagttga cagttgcctt ctcttccaat
     3721 tcttgctctg caagaatggt tttaatgtgt ttgctgatat tttgtttggt ggtttggtac
     3781 agttctgcca tgtccgcctg cgtcagccaa agctgtccgc caaattcctg caaggcaaat
     3841 tgggcagtgc cgtcgttggc ggtgtatagg atgatgctca tgcttcaatc tctgcaatca
     3901 cttcgtcaat ttcacgccgc agccgttcga ttttggcgac ggtttcgccg atttcggcgt
     3961 tgagctgttt gatgtcgata atttcgcgtg tgtcttcggc ttcgacatag ctgctgacgg
     4021 cgaggttgta gccgttgtct ttgacggttt gctgggcagc gttttgggcg atatgcggca
     4081 catcggcttt atcggcgaag agtttgacga tttcagcaat gtgttcttcg attaagacgt
     4141 tgttgttggt ttctttttta aagaagccgc ttgcgtcgat gaattggatg tcggtattgt
     4201 ctttgtgttt ggacaaaacc aggatattga cggcgatgcc ggtgccgtaa aagagattgg
     4261 gcgcaagggc aatcacggtt tccacgtagt tgccctccac cagatattgg cggattttct
     4321 gttctgcgcc gccgcgatag aaaatgccgg ggaatgagac gatggcggcg cggcctctgc
     4381 cggaaaggta gttcagtgcg tgcaggatga aggcaaaatc ggctttggat ttcggggcaa
     4441 gtacgcctgc gggggcaaag cggtcgtcgt tgattaaggt ggggtcgtcg ctgcctatcc
     4501 agttgatgga ataaggcgga ttggaaacga tggcatcaaa gggtttgctg tctttgagct
     4561 ttgggttggt cagtgtgtcg cccaattcga tgtggaattg gttgtaattg acgttgtgca
     4621 ggaacatgtt catgcgggcg aggttgtagg tggtgtggtt gatttcctgc ccgaagaagc
     4681 cttcttcgat gatgtgctcg tcaaactgtt ttttcgcctg caagagcaga ctgcccgagc
     4741 cgcaagctgg gtcgtagatt ttgttgactt tctcctgtcc gtgcaccgcc agccgcgcaa
     4801 tcagcttgga tacgctttgc ggggtgaaaa attcgccgcc ggatttgcct gcgttggcag
     4861 cgtagttgga aatcaggtat tcgtaggcat cgccgaaaag gtcgatgtgg tggttttcaa
     4921 aattgccgaa atcgagttcc gccacgcctt tgaggacggc ggcaaggcgt ttgttcttgt
     4981 cggcaacagt gctgccgagc cggctgctgg tggtgtcgaa gtcgtcaaac aggcctttga
     5041 tgtcctgttc ggacggatag ccggaggcgg agctttcaat cgcggtaaaa atttctttca
     5101 gcttggtgtt gagctcttcg ttttgatggg cttcggcggc aatattgcaa aaaagctggc
     5161 cggggtagat gaaatagcct ttaactttga cggcatcgtc tttgatttcg ggcgtgatga
     5221 tgctgtccgg catagcggcg taatcaatac tgctgtcgcc tgcctgcata tagtcggtga
     5281 agttttcgct gataaagcgg tagaaaagtg tgccgagaac gtattgttta aagtcccagc
     5341 catccaccgc gccgcgtact tcgtcggcaa ttttccaaat ttggcggtgc agttgggcgc
     5401 gttgttgcat ttcggtcatc atcgaaatcc atataaaagt taaacaaatc aaaatcgcct
     5461 gatattttca gacgattttt ttacgggcat tcaaattaag ccaaattttc cagcatccat
     5521 ttgacgtagc ggtcgacgcc tgatttgaca tcgaaaaatt cttccttata tccggcttcg
     5581 cgcaatttgg tgatgtcggc ttgggtgaag ctttggtatt tgcctttgag cgcgtcgggg
     5641 aagggaatgt agcggataag ttcttcttct accaactctt tcaagctcat ttcaggtttg
     5701 ccttcggcgg cgcggcatgc gttgacggtg gcggcggcga gttcgttgaa ctgttggctg
     5761 cggccggtac cgaggttgta gatgccggaa agttcgggat ggtcgaagaa gtagaggttg
     5821 actttggcaa cgtcttcgac gctgacgaag tcgcgggttt gttcgccgtt gccgtagccg
     5881 tcgttactgc cgaacaggtt gacgtaaccg tgttcgcggt attggtggaa gtggtggaag
     5941 gcgacggatg ccatgcggcc tttgtgttgt tcgtgttgtc cgtaaacatt gaagtagcgg
     6001 aagccgacga cttgggcggt gagaccttct ttcatgcggc gacgcaatac ttggtcgaac
     6061 aggaatttgg agtagccgta cacgttgagc ggtttttcga gttcgcgctc ttcgcggaag
     6121 atttctcctt tgccgtaaac cgccgcactg gaggcataaa ggaaggggat gcgttcgtcc
     6181 tgacaccagt ccagcaaatc cagcgtgtac tggtagttgt tgtccatcat atacaaaccg
     6241 tcgtggttca tcgtatcgga acacgcgcct tgatggaaaa cggcttcgat gttttgataa
     6301 ggtaaaatgt gttccctcac ttggcggatg aattcgtgtt tgtcgagata atgggcgatt
     6361 tcgcactcgg caaggttttt gaatttttcg cctttgctca aattatcgac ggcaacaatg
     6421 tcagtaatac cgcgttgatt aagtgctttg acgatgttgc tgccgataaa gccggccgcg
     6481 cctgttacga tgatggtcat attcggtttc ctttgttttg agtaaaatta ataaacttag
     6541 ttacaagaaa tcatcgatat tgtcctgaaa cacaaatttg cgatgatatt ccaaatattt
     6601 ttctcttcct tcgggatata ttctcatctt aattttttgc atttggattg atattaagtt
     6661 tttcccatgt gtaacggctt agtcgagtac cgcaaatcaa acgaccatca tccaagaaag
     6721 taatataacc ttgatcgaac aaccaatcat aagtcggcgt taaagctaac ccatttaaat
     6781 aatctaatgc ctctttctca tttttctcag taatacaaac catataaggt ttaatatgac
     6841 tagcccttaa caaaatctca tctgtaatta atgtaaatgg acattgcgac atatgattta
     6901 taacatcctt gcgatatttc tgctgccctt ttctactttt ctgtttaatg atttcttgct
     6961 ctacgaattg attaaattct gaagccggta tttctaattt ttctcttgat aagagttgcg
     7021 ggtgcacgat agagcgaaat tgataatcca aaaatattcg gaaataaaat aatggttctg
     7081 aatcttctat atctttgaca ggcaatagtt taagaattga taaataacta attttgggca
     7141 atacaatctt acgccaaatt gaccatatat catcctccga acggatataa cccctacggt
     7201 aacctttaga attttccaaa tggtctgata catcatggat tgaaaagaaa atattttctg
     7261 taggcagatt attaatactt aaaacgtaat catcataaaa ccttgaaata tcatgatgat
     7321 atttctgttc ttgtttgtga tattcaactt ttgcatcaac caaataacgc tgtaaatttt
     7381 tcttataaaa aaaacaattt ttctgaatga aaggatattc taccctcctt ccattttttg
     7441 tctcagtaat cccccaattt tcatactcaa aaaatgtact tagttttcga ccactatcat
     7501 caatataact acctacatat ttgcgtgctt ccccattacc accaaaaaca gataacttgt
     7561 tattcctgtg aataaaacta tcttccgcac gtaaattttg aatactatcg acaataaaat
     7621 actctatgtt ttcaatagta attctttgaa aatccggcat tttactctcc ataaagcgta
     7681 atatccattt gttttaatat agcgattaat acatcaacaa caatactgtt tcccgcttgg
     7741 cgatacattt gagtatcact aactaaaatt ttaaaatcat ctctaaaacc cattagccgc
     7801 aaacattccc taggtgttaa tttacgaata cgtccacgat tatgagtaac ataattatca
     7861 accccggcac gatgcatttt atgcatagtt tgcaataatg ggcgagctac aggcaaatca
     7921 gtttccgtac tggttttaaa attttttgtt cctcctgcca aaacataatt tttgattttt
     7981 tcagaaagat aatatttttc ctcaacgtta tttacatcaa aaataaagtc atcaaattca
     8041 gattcgcggg ctgcctgaaa aataaaatca ccatgccaat taaattgttg atttgctttt
     8101 tggcataagg caatttctcc attaatctgt gtataacgtt tttgtctatt tttagaactg
     8161 gtaacaaatt tcgcaccctt ttcacgtaaa aaatatttgc tatcagtata gtcctccaaa
     8221 aaatcttgca tagtatgttc taattcaatc ttttcaggaa actgaaaacc atttatagga
     8281 ggggtatgaa agccaacaac aaaaatacgc tcacgatgtt gaggaatccc ataatcctta
     8341 ctattcatta tttggaaata taagtcataa ccaagtgaat aaaaaacact ttttacaact
     8401 ttccaagttt ttccattatc atgattaagc aagcccttta cattttcata aataaatatc
     8461 tttggttgga cttcatccac aacgcgagca aattcataga ataatgttcc tcgtgtatct
     8521 tctaatcctg cacgtttgcc aaccatggaa aatgcttggc aaggactgcc tccaactaaa
     8581 atatcaactt gatttctaaa ctttctcgca tcaaattgag taatgtcgtt atgccaaaaa
     8641 tcttcattta atttatagtt tccaagataa ctttttttaa cgtatggata aatatctcct
     8701 gaaaaaacaa tggtatggtt taaatttaat cggtggaatg cctgttcaac cgcaccaatc
     8761 ccgctgaata cggttgctaa tctaatatgt gaatcaggtt taagaaaagt tttagatttc
     8821 caaccttgtt gactgggaaa gagcaaagtt ttttgtaatc gagtatcgtg tgtctgtgcc
     8881 attgtcgaaa tagtcatact tatatcgttc tgtttatctt atcaatatga aaactacatt
     8941 gttgattgcc ctgacaatgc cttgatcaat tcggcaaacg agcaaaccgc cgtaccgagt
     9001 ttcgccacga caaccccggc cgcagtattg gcaaggtaca tggcttcggg catggtgcag
     9061 cctgccgcca aacccaagcc cattccggca atgacggtgt cgcccgcacc ggatacgtcg
     9121 taaacttctt gggcgcgggt gggctggtaa atcggttcgc cttcgctgaa caaggtcatg
     9181 ccttcttcgc ttcgggtcag taaaacggcg gtcaggtcga ggtggcggcg caggttttgc
     9241 gctttttcgg tcagctcgct ttcgtttttc caactgccga ccacttcttt caattcggcg
     9301 cggttaggcg taatcagagt tgcaccgaca tatttttcgt aatcgtcgcc tttggggtcg
     9361 attaagacgg ttttgccggc gtgtttcgcc caatcgatca tatcggagat atgcgacagg
     9421 ccgcctttgc cgtagtctga aaaaatgatt gcgtcgtatt cgggcaagat ttcgcggtat
     9481 ttctgcttga tttgttccaa cacttcgcag ttgggatgtt cttcaaaatc aagacggata
     9541 agctgctggt tgcgggcgac gacgcgcagt ttgacggtgg tggcgatttg tttgtcgcgc
     9601 atcagatagg aggcgacgcc gtcctgcacc atcagcgcat cgagcgcgtc ggcggcttcg
     9661 tcgttgccgg ttacggacaa cagccctgcc ctgccgccca acgaagcgat gttgcgcgcg
     9721 acatttgccg ctccgcccgc gcgttggtcg attcgtccga ttttcgccac cggcacgggg
     9781 gcttcgggcg aaatacggga cacatcgccg aaccaatagc ggtcgagcat cacgtcgccg
     9841 acaaccagga ctttggcttg cgcgaaacgg gatttgaggg tttcttgttg gaacttggcg
     9901 gacatattgc ctccgttgta ttttttcaga cggcctcagc ctgtaaggcc gtctgaaaat
     9961 cagtttgctt caaggtttgc cacgcggttc acttcgcgca atacggctac cggatcggca
    10021 gcttgggtta caggacgacc cattaccaaa taagtcgaac cggcagccaa ggcttcggcc
    10081 ggtgtcatga tgcggcgctg gtcatcatta ttgccggcaa cgtccaagcg gatgccgggc
    10141 gtgaccaaga caaaatcctg tcccaattcg cggcgcagcg gcgcggcttc ttgggcggaa
    10201 cagaccacgc cgtccaagcc cgaactttgc gccagttttg ccaagcggat gacttgttct
    10261 tcaggggcgg tgttcaaacc gatttccgcc aaatcacttt gttccatgct ggtcaacacg
    10321 gttacgccga ttaagagcgg cttcgtgccg tatccggcaa cggcttctgc tgcggcttcc
    10381 atcatacggc ggccgcccga tgcgtgcata tcgaccatcc aaacgcccat atcggcagcg
    10441 actttgcagg cttgcgcgac ggtgtgggga atatcgtggt atttcaaatc gaggaaaagt
    10501 ttgaaacctt gattgattaa gctttctgcc aaattgcgtc ccgtcgcggt aaacagctct
    10561 ttgccgattt tgatttgaca caatgtcggg tcaaggttgc ggacgaatcc gagcgtgtct
    10621 ttttcgttgg aaaaatcaag ggcgacgata acgggggtgc gttgttgcgg agtttggaag
    10681 tcggagatta aaggattcat ggtgttttcg tttcgggttt tcagacgacc tttatcgtct
    10741 ttttcgcagt atagtggatt aactttaaac cggtacggcg ttgtctcgcc ttagttcaaa
    10801 gagaacgctt ctctaaggtg ctgaagcaca aagtgaatcg gttccgtact atctgtactg
    10861 cctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataacaac gtaaattcaa
    10921 accgtaggtc gggcattgat gtccgaccta cgcggactca agcctccgac aaactgtttc
    10981 ggataaattt gaaccggtac atcagccgtc attcccgcgc aggcgggaat ccattgataa
    11041 aactcagttc atcagattta aacagcgatt gcccaaattc agaaatggat tcccgcctgc
    11101 gcgggaatga cgccaaatga ttatttttcg gttgcaacac gctgtttatc aaaaacaggt
    11161 cgtctgaaaa cctgattttc tgttttcaga gtgcgaagcc tgcccgtggg tacgaccttt
    11221 tcgatttatt tgcccaatgc gcccaatact tcggctttca cggcttctac tgcttgggtg
    11281 ccgtcaactt tgatgtattt aggcgcgtgt tcgccttcca gtttgctgta aaaatcgacc
    11341 aaaacttcgg tttgctcgtg gtaaacggca aggcgttttt tcacggtttc ttctttgtcg
    11401 tcgtcgcgct gaatcaaatc ttcgccggtt acgtcgtctt tgccttcaac tttgggcggg
    11461 ttgtaggtaa cgtggtaagt acggccggaa gccaaatgca cgcggcggcc gctcatgcgg
    11521 tcgacaatca cgctgtcagg cacatcgatt tcaacgactg catccaaatc cacgcctgct
    11581 tcaaccatcg cttcggcttg tgccaatgtg cgcgggaaac cgtcaaacaa gaaaccgttt
    11641 ttgcagtcgt cttgcgcgat gcgttctttg accatgccga taatgatgtc gtcgcgcacc
    11701 aagccgcctt cgtcaatgat ttttttcgct tccaaaccca agggcgtgcc tgccttaatc
    11761 gcggcacgga gcatgtcgcc ggtagagatt tgcggaatgc cgaacgctgc ggtgatgaat
    11821 tgcgcctgag tgcctttgcc cgcgcccggc gcgcctaaaa gtaatgcttt catgaagtga
    11881 tcctttgaaa tgtgtttatt ggaatcaaac agatggttta cgattctaca cggcttggca
    11941 caatagttca aaaaccaact gcacaaactg ccatttgcaa gcaaatgttt tgcatacctt
    12001 tgccgagtct gatattcaca agccggcaac tttttgggac aatcccccaa aatttcaaaa
    12061 catcacaccc taccaaaaag gaacatccgt gaaacgaatt tttctgtttt tggctaccaa
    12121 tatcgctgtt ttggtcgtaa tcaacattgt tttggcggtt ttgggcatca acagccgggg
    12181 cggcacgggc agcctgttgg cgtattccgc cgtcgtcggc ttcactggtt cgattatttc
    12241 gctgctgatg tccaaattta tcgccaaaca atcggtcggc gcggaagtta tcgacacgcc
    12301 gcgcaccgaa gaagaagcct ggcttttgaa cactgtcgaa gcccaagcgc ggcaatggaa
    12361 cctgaaaacg cccgaagtcg ccatctacca ctcccccgaa cccaatgcct ttgccacggg
    12421 cgcatcgaga aacagctccc tgatcgccgt cagcaccggt ttgctcgacc atatgacgcg
    12481 tgacgaagtg gaagccgtat tggcgcacga aatggcacac gtcggcaacg gcgatatggt
    12541 tacgctgacg ctgattcaag gcgtggtcaa tacctttgtc gtgttcctgt cgcgcattat
    12601 tgccaacctg attgcccgaa acaacgacgg cagccagtcc cagggaactt atttcctggt
    12661 cagcatggta ttccaaatcc tgttcggctt ccttgccagc ttaattgtca tgtggttcag
    12721 ccgacaacgc gaataccgcg ccgatgcggg cgcggcaaaa ctggtcggcg cgccgaaaat
    12781 gatttccgcc ctgcaaaggc tcaaaggcaa cccggtcgat ttgcccgaag aaatgaacgc
    12841 aatgggcatc gccggagata cgcgcgactc cctgctcagc acccaccctt cgctggacaa
    12901 ccgtatcgcc cgcctcaaat cgctttaaac cgatttgaaa cggcaaaaac cgtacgcgca
    12961 agcagtacgg ttttttgtat atgctgtctg aaaacatcag gtttccatac cgacaccaac
    13021 cgtatccatt tccgcaccgg atacgccgca ttgatatacc ctgataaacc gtatccggac
    13081 agaaaatata gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaaa
    13141 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattt atactgtctg
    13201 cggcttcgtc gccttgtcct gatttttgtt aatcccctat atcagataaa acagcggtaa
    13261 aattgccgtc tgaaaccaac tctgcgccat acgggacacg ctctgcacgt ctatttgcct
    13321 ccgatatatc ctccctactg taggaacgtc tgccgatttg cacgaaatcg gcacctctag
    13381 cacccttcag gatggcggac gttatccgtt cttgggattc tgcaagacgg tcgacggcat
    13441 tatacagacg ctcggtctga tacccttccg aagcaacctg cttcaggtat tccaaataac
    13501 cgtaacccaa cgcccctccg acaagcaggg tcgtcaacgc taccagcgtt gccgaacgga
    13561 ttttaggggg catatcttga agcatatctc taccgaaccg aaccaaagtc tctttaaagt
    13621 cggactctac acgcgtagaa ccctcggaaa tcttgaagtt caactcatac tgcttccgtt
    13681 tgtccgctgt caaccgcttg aggttgtcat ccccgtattg gacggttgac acaaccctgt
    13741 acagactgtc ctgtatttcg accagcgtct gtgcaaagtt ggcggtcata actggattcc
    13801 caacccttgc cgccgattct gatactcaaa acggtatcca ggcgttccag cagggcttcg
    13861 ggagacagca gtgcgtattc ttcaatattt tccataggtt gattatagca gaaccttata
    13921 cctgttacag acaaggttgt cgggatttga acgtccgtcg agcttggttc gtatagagcc
    13981 gtagggggtg tttagggctt tggcggcagc ttgtacgctg gggaacgtga gtgtttcgcc
    14041 tgtggcggtg tcgaggatgg agacaggttt ttggttttgg ggaggcgtat tttccttcat
    14101 tttaagtttc ccgcactccg gacacccata ctttgacttt atagctgtgt cgtaatacaa
    14161 aagttggaca accccgtgcg taggacatct aacactggaa ggtttataaa tgccactgaa
    14221 ctccaacaat tctaaatgtg gaaatttctc ttgaaagcgt gatttggcgg tatcgaaagt
    14281 cattcgctta cccataattt gtctccatag gttatgtgca aatggattac acaaccaaaa
    14341 tatagaaatt ttcaaatttt ttgagaccta cctgaaatcc tgcgcaagca caatgtaaag
    14401 aaaaatggta tacgtcttta ctaaatcttt acataaatta actctaaccg ggatttgaac
    14461 gtccgtcgag cttggttcgt aggatttagg gtgttgtgaa ccttacctat cgtacccaag
    14521 cactttgtac ctgtttgaaa taaggttgtc gggcgacgtt cgacccttga gcctgtgatt
    14581 gatttgttgg aaatgtacac ctaaagcagc acctgcggca gtaacgcttg ggaacgtgag
    14641 tgtttcgttg gtcgttgtgt cgaggatgag gaggggcttc cctacatttg gaggtgtttg
    14701 ggtttttgac atcttagcac actcaggaca cccgtacttt gactttataa gggcattaaa
    14761 tgtcgaataa cgaacgttcc cgtgcatagg acaattaacg gttactgggt aattcacacc
    14821 gtcaaagccg gttagagaga tattagggaa tatatcagta agttcagaag ctgcttggtt
    14881 gaaagatttg gccatttgag actccaaaat tgattgtcaa atggatttaa tagcaaattt
    14941 ataaaaatgt caaatttttc gagaagcacc tgatagtggc gaggggtata cactgttaaa
    15001 aagtgtatac gtcatttagt gcagatgtga accttatacc tccctgcaag taaagtctct
    15061 gtgtgaaccc gaccttttat acggtcagtg atagctgctg ggtttatacc caatgacctt
    15121 gcagcagatg ctcttgaggg gaatgtaagt gtttcgccag ttacagtatc ctccaatact
    15181 gtatttttag ataatttatc tattgtatct tctggaattt tgtgggaatt gacaccataa
    15241 gatgcacatt ttggacaccc acttttagag tccaacatac ttttgaatgt agagcaagta
    15301 accctcccgt gtataggaca gacgattacc gtcgggtaac gaacgccatt gaactccaca
    15361 agattcaaat gtggaaattt agaggttagc ttagaaactg cttgagtaaa tgtaagtgcc
    15421 atagtggttg tgaacttact ttaccaacct ataacgatac ctaatcaatc ggtcaaacgg
    15481 agtgcgtccg cttaaacgta cagctaaagt gctgttggag caaccgataa atttactggc
    15541 agccagtgta cttgcaaact cgtagatttc ccctgtctct gtgtcctcca acctaatggc
    15601 aacggcgttt ggagagggct tttcgggatt tcgagtacat tcaggacatc ctgttgagga
    15661 tttcatggca gcttgtagat tcccgtaaac aaccgaccca tgcaaagggc attggattgt
    15721 taccggttcg tagtttttag tgaacttcaa aaccgtctta tcaggaaatt tactggaaaa
    15781 ccttaactga acatcttgta gcgagagtga ttttggcatt atagaccttt ctaaaatgaa
    15841 agcattatac aaccaaaata tagaaatttt caaattttct ctgaacgcac tggtatccga
    15901 tggagctaaa tgtaaagaaa agggtatatg tccttactaa atatttacac attttaactg
    15961 tatagtgttt gacaaaaaac aggcataggc gcataatgca cccgtagtta gatagatagt
    16021 aaggagataa ccaatgactt agcacctgcc cccctctctc tcgcgcaccc cgcccctcgc
    16081 ttgcggctaa tgggctttgc gcgacatata cactttcatc tcctgcatgg cttaaccgcc
    16141 tatgcaatag tacacacatc aacaaatcag gctggcacgt cttggcattg gctgacgtac
    16201 cattgtagcc cgaaatgttg ataccctact atttatgacc aacggcggat tagggcttca
    16261 gacggcaatt ttactgcaaa accttatgcg aacggatgat gcagcacaat cgtttcttct
    16321 cggtcggggc cggtggagac gatggcgacc ggcgcgccgc agacttcttc aatccgtttc
    16381 aaatatgctt tggcgttttc gggcaatgcg ccgtagtcct tcacgccgaa agtggattcg
    16441 cgccagccgg gcatggtttc gtaaatcggc ttgcaggttt ccaccgcatc ggaaccgcaa
    16501 ggcaggatgt cggttttgcc gccgtcgggc aattcatagc cgacgcagat attgatggtt
    16561 tcaacgccgt ccattacatc gagtttagta atacacatac cggaaatgcc gttgatttgg
    16621 atggagcgtt tcagggcggc ggcatcaaac cagccgcagc ggcgtgcgcg tccggttacc
    16681 gaaccgaatt cgtgtccgcg ttctgccaaa cctacgccta cttcgtcgaa caattcggtc
    16741 gggaacgggc ccgaaccgac gcgcgtggta taggctttga cgatgcccaa aacataatcc
    16801 agcatttgag gacctacgcc cgcgcctgcc gaagctgcgc ccgccagaca gttggacgag
    16861 gtaacgaagg gataagtgcc gtagtcgatg tccaacaacg caccttgcgc gccttcaaac
    16921 agcagttttt cgccgttttt gtttttctcg ttcaacacgc gggacacgtc ggtaatcatc
    16981 ggcgcaatgc gcggcgcgac tttttcgata accgccatca cgtcttccgc tttaaccggc
    17041 tcggcattgt gcagatgttg cagttggaca ttgtaatagg caaggacggc atccagtttt
    17101 tcacgcagtt tttcaggatg cagcaaatcg gcggcgcgaa tggcgcggcg tgccactttg
    17161 tcttcgtagg cagggccgat gccgcggccg gtcgtgccga ttttgccttt gccgcgcgat
    17221 gcttcgcggg cttggtcgag cgcgatgtgg taaggcagga tcagcgggca ggtcggcgcg
    17281 attttcagac ggccttcgac gtttttcacg cctgccgcgt tcaactcgtc gatttcgccc
    17341 aacagggctt cgggggagac gacaacgccc gaaccgatga agcagtccaa actttcatgc
    17401 aggatgccgc tcggaatcag gcgcaaaatg gtttttttgc cgccgacaac caaggtatgg
    17461 cccgcattgt ggccgccttg gaagcgcacc acgccgccgg cttcttccgc cagccagtca
    17521 acgattttac ctttaccctc gtcgccccac tgtgcgccga ttactacaac atttttagcc
    17581 atagccatat aacctatcga tattaaaaat tattgcggaa atccccaaac cggtgcggct
    17641 ttgcgccccg ccacgatttg cctttcatcc gccatgaacg ggtatttagc gtttcacgac
    17701 ctgccaaacg ccgtccgtct ttttcagacg gcctgcaagc tcttccgaaa cattgtgtcc
    17761 gataccgtaa tcgattacga cacactgccc ttgttcacgc aaggcttcga ccgcttcgtg
    17821 cgccgcttcg gcatcttccg catcgaccaa cacggcgggc tgccgttcga tggcgggcaa
    17881 acgcccgata aagctgcgca agtcgaaact gaatcccgtt gccgggcgcg ccctaccgaa
    17941 atatccgccc aatccgtcat aacgcccgcc gcgcgcgacc gcgtcgtgga aatcggcggc
    18001 ataggcggca tacagcaagc ccgtgtggta attgtcgaca cgcagctcgg acaagtcgat
    18061 atggatttca caatcgggga atgcgtcgca caccgcctgc aattcgccca acgcgccgcc
    18121 gaccgccgac aaatccggca accgtccgcg cgcgtcggac aacacttcac gcccgccgta
    18181 caggcgcggc aacagcgaga atgcttttgc ccacatgccg tccagcttcc aagccttgac
    18241 ctgcgcttcg accgccccgg tatctttatc ctgcatcaag gcaagcagcg ttgcggactg
    18301 ccccgcatcc aaatgtgccg catcggacaa ggcgcgaaat atgccgatat gccccagcga
    18361 aagcagcact ttgcccatat cggcaatttt catgcttttc agcatcaggt ctatcagctc
    18421 gatgtcgcca cggatgtcag caaaaccgta catttctgcc cctgcctgca agggttcgcg
    18481 catattcagc agaccgtcgg gctgcgcgtg caacaccgga ccggcataac acaaccggtt
    18541 aatcccttgg ttggcggata aaagatgggc atcgatacgc gccacctgcg gcgtgatgtc
    18601 ggcgcgtatg cccaactgcc tgccgctgag cctgtccgtt accaaaatgg ttttcaggga
    18661 aagccccgca tcgatatgcg tcagcaggga atgtgcgtac tccatcagcg gaggctgtac
    18721 cagttcataa ccgtgtacgc ggaacagtgc caacaactgc tccctcgcgc tttcaagctg
    18781 ccgcgcgttc gtgggcagta cgtcggcgat atgttccgga agctgccatg tctgcatgag
    18841 aatctgcctt ctattttatc ctgtaaacac aaaagactgc ccgatccgca atcagacggc
    18901 atctttgccg caacgcatca cgccgctgtc aaaacggcgg tgcttctttc cgattcagaa
    18961 tccgaacagc ccctgtccga cggctcaatg ccgtcaagtc gttaaaacca aactttacca
    19021 taaaatacac acaatctgaa tatactttta taccctgtat ccgccgtctg acggcacggc
    19081 atttttttac gatataatgt gcaccattat ttctcatcac ttcctgctgc cgttttctgc
    19141 ttcagacggc atttttgttg attgaacact tatgctgctc gacctcaacc gcttttcctt
    19201 tcccgtcttc ctgaaagaag tccgcctgct gaccactctt gccctgccca tgctgttggc
    19261 gcaggtcgcg caggtgggca tcggttttgt cgatactgtg atggcgggcg gtgcgggcaa
    19321 ggaagacttg gcggcggtgg ctttgggcag cagcgcgttt gccacggttt atattacctt
    19381 tatgggcatt atggcggcgc tgaacccgat gattgcccag ctttacggcg cgggtaaaac
    19441 cgacgaagtg ggcgaaacgg ggcggcaggg gatttggttc gggctgtttt tgggcgtgtt
    19501 cggcatggtc ttgatgtggg cggcgattac gccgttccgc aactggctga ccttgagcga
    19561 ttatgtggaa ggcacgatgg cgcagtatat gttgttcacc agcttggcga tgccggcggc
    19621 aatggtacac cgcgcgctgc acgcctacac ttccagcctg aaccgcccgc gcctgattat
    19681 gttggtcagc tttgcggcgt ttgtgttgaa cgtgccgctg aactatattt tcgtttacgg
    19741 caaattcggt atgcccgctt tgggcggcgc aggctgcgga ctggcgacga tggcggtgtt
    19801 ttggttcagc gcgctggcat tgtggattta tatcgccaag gaaaatttct tccgcccatt
    19861 cggactgacg gcgaaattcg gcaaaccgga ttgggcggtg ttcaaacaga tttggaaaat
    19921 cggcgcaccc atcgggctgt cttatttttt ggaagccagc gcgttttcgt ttatcgtgtt
    19981 tttgattgcg cctttcggcg aggattatgt ggcggcgcag caggtcggca tcagtttgtc
    20041 ggggattctc tatatgattc cgcaaagcgt cggctcggcg gggacggtgc gcatcggctt
    20101 ttcgcttggg cggcgcgaat tttcgcgggc gcgttatatt tcgggcgtgt cactggtgtt
    20161 aggatggatg ctcgccgtga ttaccgtgct ttccttggta ttattccgtt cgccgctggt
    20221 aagtatgtac aacaatgatc cggcggtttt aagcatcgcc gccaccgtct tactgttcgc
    20281 cggcttgttc caaccggcag acttcaccca atgtatcgcc tcctacgcct tgcgcggcta
    20341 caaagttaca aaggtgccga tgttcatcca cgccgccgcc ttttggggct gcggcctgct
    20401 gccgggctat ctgctcgcct accgtttcga tatgggcatt tacggcttct ggacggcatt
    20461 gattgcctcg ctcaccatcg ccgccatcgc cttggtgtgg tgcttggaat tgtgcagtag
    20521 ggagatggtc agatcgcata aggccgtctg aaaacgcagt acacttcaat tcaaatacag
    20581 ttaaagttca aaccatgcaa cccatccgat accgaaccga ccttaccccc tacaacacct
    20641 tcggtcttcg cgcccaagcc cgggccttta tcgcgctcga acatgccgac gagttgcgcg
    20701 acatcgtccg actgctggag ttcgaccgcg atactgtttt atggctgggc ggcggcagca
    20761 acatcctttt gatgcaggat tacgccggac tggtcgtaca catggaaaac aaaggcatac
    20821 gcgagattgc gcgttcagac ggcatggttc tgattgaagc gcaggcgggc gaaatttggc
    20881 acgattttgt cctgcacacc gttgcgctgg gtttgagcgg tttggaaaac ctgagcctga
    20941 ttccgggtac ggtcggcgca tcgcccgtgc agaacatcgg cgcatacggc gtggaggcga
    21001 aagacgtgat tcacagcgtg cgctgctttg atttggatac ggagaccttt gtcgagcttg
    21061 ccaatgccga ctgccgcttc gcctaccgcg aaagcctgtt caagcaggag ggtaaagggc
    21121 gttatgtgat tgtttcggtc gtatttgcat taaaaacgca ttttgtgccg actttgggtt
    21181 acggcgattt ggcggccgcc gttgccgaac tgagcgcggg cagggtcccg acggcgaaag
    21241 atgtttccga tgcagtgtgt gcaatccgca acagtaaact tcctaatcct aacgtgcttg
    21301 gcaatgtcgg cagtttcttt aaaaaccccg tcgtcagcgc agaaaaagcc gccaccttgt
    21361 tgcagcggca tcctgatatg ccgcgctatc cgcagcccga cggttcggtc aaactcgccg
    21421 ccggctggct gatcgaccaa tgccgtctga aaggcttcca aatcggcggc gcggcggtac
    21481 atgacaggca ggctttggtc ttagtgaaca aaaacaacgc ctcggcaaac gatgtccggc
    21541 agttggcgca acacatcaaa tttacagtat ttgctcggtt tcaggtagaa ttgcacgccg
    21601 aacctaattg gctgcctgct tcgttcagcc tgtaaatcca aggagtatgc ccgtgacccg
    21661 ccccgccaaa atcaatactt acacacgcat catcgacgcc agccttgcgc ttttcaacga
    21721 ggaaggcgag cgcaacatca gcaccaacca tattgccgcc cacttgggca tcagtccggg
    21781 caacctctat taccacttcc gcaacaaaga cgaaatcatc gtccaactgt tcaaacgtta
    21841 cagcgaagcc ctgctggcat acctgaatga agccgtgttg ccgtctgatg tggaagactc
    21901 catcaattat atggccggta tttatgatgt gatgtgggaa taccgcttcc tattcagcga
    21961 cgtgaacacc ctgcttgcac gcagtgccga attgttgggc gaacacaata ccttcaccca
    22021 agccaaagtc tccccgctct tggtcaacct gctcacccaa ctcaacggtc tgaacatcat
    22081 ccaagccgac caaaccgcca tgaacgatct cgccgtcaat atgtggatgg tcacgaaata
    22141 ctggttcgac ttcgacagct ccctgcgcgg ccgcaccaag ctgaccgaag actccaaagc
    22201 acgcggcatc cgccgcacct taagcctcct tcgtccttat cttttgcccg aacaccgcaa
    22261 gaaatacgac cggaaaatcg gcaacggcaa cccgtaaacc cacagcgtcc gaatcagcct
    22321 tcagacggca cttacccgac ggtatcagcg aatgcgcgca gtccccccgc cccatatctc
    22381 catcacaggc ctaggttacc tcggcctgcc gctggcacaa aagttttacc aacacggcag
    22441 ccgcgttgcc gccgtcaaac gcagcctgac ttcggacgat atcaatctgc ccatacacct
    22501 cgataccatc gacctcaatc aagacagcgc gtttcaaagc gcgaaccttg cccgagatac
    22561 aagcttttgg cggcaccatg ccgacaaacc cgtctggttc ttcctgctac cgccctcctc
    22621 gctgacgcat tacgccgaca ccgtcaaaca atgggcagaa cttgcccggg cgtgcaacgt
    22681 gcaacacctg attttcacaa gcagcaccag cgtttacggc gatacagcgc gcgaatgcga
    22741 cgaaaccgcc acacccgatc cgcaaaccga gtccgcccgc caaatcctcg ccgccgaaca
    22801 acacctgccc gacagcggca tctccaacat cgacatcctg cggctgggcg ggctttattg
    22861 cgccgaacgc catcccgtca gccgccttgt tcaaaagcaa aacatcccgg gcggcaaccg
    22921 ccccatcaac atcgtccacc gtaatatcgc cgtcgaaagc ctgtttcaga cggcatttaa
    22981 ccccggcggc aggcggctga aaaacattat cgaaccgcgc cacccgacac gacgcgaatt
    23041 ctacacggaa gaagccgcca aactcggcct gcccgcgccg gatttcgcac ccgacgacag
    23101 cgtgggcaaa atcatccgta ccgtttgcga taacggctta agcctgtaaa ataagcggca
    23161 acagcaacaa acaggcattc tccgccccaa tcagaaaata tgtccgcctt cctccccatc
    23221 atcaaccgcc tgattctgca aagcccggac agccgctcgg aacttgccgc ctttgcaggc
    23281 aaaacactga ccctgaacat tgccgggctg aaactggcgg gacgcatcac ggaagacggt
    23341 ttgctctcgg cgggaaacgg ctttgcagac accgaaatca ccttccgcaa cagcgcggta
    23401 cagaaaatcc tccaaggcgg cgaacccggg gcgggcgaca tcgggctcga aggcgacctc
    23461 atcctcggca tcgcggtact gtccctgctc ggcagcctgc gttcccgcgc atcggacgaa
    23521 ttggcacgga ttttcggcac gcaggcagac atcggcagcc gtgccgccga catcggacac
    23581 ggcatcaaac aaatcggcag gaacatcgcc gaacaaatcg gcggattttc ccgcgaatcc
    23641 gagtccgcaa acatcggcaa cgaagccctt gccgactgcc tcgacgaaat aagcagactg
    23701 cgcgacggcg tggaacgcct caacgaacgc ctcgaccggc tcgaacgcga catttggata
    23761 gactaacctt cagacggcat ccgacatgaa cagccctttt cacaacatcg gcatcgtaac
    23821 ccgccccaac acgcccgaca tccaagacac cgcacacacg ctgattacct ttttgaagca
    23881 gcacggcttt accgtctatc tcgacgaagt cggcataaag gaagactgca tctacaccca
    23941 agacaccgtc ggctgccata tcgtcaacaa gaccgaactg gggcaatact gcgacctggt
    24001 cgccgtttta ggcggagacg gcacctttct ctccgtcgcc cgcgaaatcg ccccgcgcgc
    24061 cgttccgatt atcggcatca accaagggca tttgggcttc ctgacccaaa ttccccgcga
    24121 atatatgacg gacaagctat tgcccgtttt agaagggaaa taccttgccg aagagcgcat
    24181 cctgattgag gccgcactca tccgcgaagg caaaaccgcc gaacgcgcca tcgccctcaa
    24241 cgatgccgtc ctctcccgtg gcggtgccgg acagatgatt gagtttgaag tcttcgtcaa
    24301 tcgggaattc gtctataccc agcgttcgga cgggctgatt gtctccaccc ccaccggatc
    24361 gaccgcctat tcgcttgccg ccggcggccc catcatgcag gcaggtttgc acgccttcac
    24421 actcgtaccc atctgcccgc aatccatgac caaccgcccc atcgccattc ccgacacgtc
    24481 cgaaatcgaa atcctcgtta cccaaggcgg cgacgcgcgc gtccatttcg acggtcaaac
    24541 ccatatcgac gtgcaaaacc tcgaccgcat caccatccgc cgctaccgaa accccttacg
    24601 catcctacat ccgaccgact accaatattt caaaaccctg cgccaaaaac tgcactgggg
    24661 cgagcaatta gtctaagccg gcctttaccc gaaagtatcc atgaatccca aaatttcctc
    24721 cgaccataca gaagatgccg tgcgcctctc caaacgcatg gcgcaactgg ggctttgttc
    24781 gcgccgcgaa gccgacggct acatcgaaca gggctgggta acggtcaacg gcaaaaccgc
    24841 cgtactcggt cagaaagttt caccggcaga ccgtatcgaa ctgaacaaga aagcccacga
    24901 acagcaggcg gcacgcatta ccatcctgtt gaacaaaccc gtcggctatg tcagcgcaca
    24961 agcggaaaaa ggctataaat ccgccgccga actgattacc cctgaaaatc actgggaagg
    25021 cgataccggc cgcatccgtt tcgatccgaa acacaaaatc ggcctcgccc ccgccggcag
    25081 gctggacatc gactcggtcg gattgctggt attgactcag gacggccgta tcgccaagca
    25141 gcttatcggc gaaaacagcg gcagtgaaaa agaatatttg gtgcgcgtgc gcggcaaatt
    25201 ggacgaaaaa ggacttgcct tactgaatca cggattgagt ttggacggcg agaaactgcg
    25261 tcccgcccaa gtagaatggc aaaacgaaga ccaactgcgc ttcgtgttga aacagggtaa
    25321 aaagcggcaa atccgccgta tgtgcgaact ggtcgggctg cgcgtcgtcg ggctgaaacg
    25381 catccggatg ggcaaggtca aactcggcag gctgccgccc ggcaaatggc gttatctcgc
    25441 ccccggcgaa tcgttttaaa ta
//
LOCUS       DAWIVB010000009        24812 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWIVB010000009 DAWIVB010000000
VERSION     DAWIVB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.009"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            59..1132
                     /locus_tag="WIX60_000269"
     CDS             59..1132
                     /locus_tag="WIX60_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ5304598.1"
                     /translation="MRPFFVGAAVLAILGALVFFINPGAIVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNIVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRAFLMNVNPIFFITVPAILTAA
                     VFVLYLFTFIPIFRANAFTDDPE"
     gene            complement(1179..3368)
                     /locus_tag="WIX60_000270"
     CDS             complement(1179..3368)
                     /locus_tag="WIX60_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ5304599.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDMARILSVQTAFVEEKPLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSYHGQASHSEFVLNADQQQASDEIQTAFGSFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGYTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRTFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWAQVLQQNRDGKIRWSVDVDPQEA"
     gene            3506..4288
                     /locus_tag="WIX60_000271"
     CDS             3506..4288
                     /locus_tag="WIX60_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ5304600.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(4701..6635)
                     /locus_tag="WIX60_000272"
     CDS             complement(4701..6635)
                     /locus_tag="WIX60_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ5304601.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPDIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6701..7879)
                     /locus_tag="WIX60_000273"
     CDS             complement(6701..7879)
                     /locus_tag="WIX60_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ5304602.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLSTGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8105..8491
                     /locus_tag="WIX60_000274"
     CDS             8105..8491
                     /locus_tag="WIX60_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ5304603.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVK
                     STLLMNAQNLERYYRQKGTFENYDQTKLKQNKYFKITLSKSPDHFTLQADPDPMTNEG
                     ETCIVTLNDGGTLSAAGNGQSCPDFD"
     gene            8775..9821
                     /gene="adhP"
                     /locus_tag="WIX60_000275"
     CDS             8775..9821
                     /gene="adhP"
                     /locus_tag="WIX60_000275"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ5304604.1"
                     /translation="MKMQAVVVNKNVAGDVEVVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(9986..13471)
                     /gene="smc"
                     /locus_tag="WIX60_000276"
     CDS             complement(9986..13471)
                     /gene="smc"
                     /locus_tag="WIX60_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ5304605.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWRQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTIAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHYWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIVTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNSKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCRLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            13720..14442
                     /gene="lpxH"
                     /locus_tag="WIX60_000277"
     CDS             13720..14442
                     /gene="lpxH"
                     /locus_tag="WIX60_000277"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ5304606.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAAGQARAIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            14711..16297
                     /locus_tag="WIX60_000278"
     CDS             14711..16297
                     /locus_tag="WIX60_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ5304607.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16466..16669)
                     /locus_tag="WIX60_000279"
     CDS             complement(16466..16669)
                     /locus_tag="WIX60_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ5304608.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPGNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            16785..17282
                     /locus_tag="WIX60_000280"
     CDS             16785..17282
                     /locus_tag="WIX60_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ5304609.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIPSLRKQFPEFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17364..18557
                     /locus_tag="WIX60_000281"
     CDS             17364..18557
                     /locus_tag="WIX60_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ5304610.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVKVL"
     gene            complement(18961..19896)
                     /gene="hemC"
                     /locus_tag="WIX60_000282"
     CDS             complement(18961..19896)
                     /gene="hemC"
                     /locus_tag="WIX60_000282"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ5304611.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20015..20524
                     /gene="ybeY"
                     /locus_tag="WIX60_000283"
     CDS             20015..20524
                     /gene="ybeY"
                     /locus_tag="WIX60_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ5304612.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQED"
     gene            20532..21356
                     /locus_tag="WIX60_000284"
     CDS             20532..21356
                     /locus_tag="WIX60_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEZ5304613.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(21485..22864)
                     /gene="nhaC"
                     /locus_tag="WIX60_000285"
     CDS             complement(21485..22864)
                     /gene="nhaC"
                     /locus_tag="WIX60_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ5304614.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSSFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23155..24378)
                     /locus_tag="WIX60_000286"
     CDS             complement(23155..24378)
                     /locus_tag="WIX60_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ5304615.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         5681 a         7081 c         6386 g         5664 t
ORIGIN      
        1 ggacggcttg atttgagttt tggtattttt gcccgacggg gtgaaaaata cagttgctat
       61 gcggccgttt ttcgtcggcg cggcggtgct tgccatactc ggtgcgctgg tgtttttcat
      121 caaccccggt gccatcgtcc tgcaccgcca aattttcttg gaacttatgc tgccggcggc
      181 atacggcggt tttttgactg cggctttgtt ggactggacg ggtttttcgg gtaacctgaa
      241 acctgtcgcg actttgatgg cggcattatt gctcgccgca tccgctatac tgcccttttc
      301 gccgcaaact gcctcgtttt tcgtcgccgc ctattggctg gtgttgctgc tgttctgcgc
      361 ccggctgatt tggctagacc gaaacaccga caacttcgcc ctgctaatgt tacttgccgc
      421 gttcactgtt tttcagacgg catatgccgt cagcggcgat ttgaacctgt tgcgcgcgca
      481 agtgcatcta aatatggcgg cggtgatgtt cgtatccgtg cgcgtcagta ttcttttggg
      541 cgcggaagcc ctgaaagaat gccgtctgaa agaccctgtt tttattccaa atatcgttta
      601 taaaaacatc gccattactt tcctgctctt gcacgccgcc gccgaacttt ggctgcccgc
      661 gcaaaccgcc ggttttaccg cgctcgccgt cggcttcatc ctgctcgcca agctgcgtga
      721 gcttcaccat cacgaactct tacgtaaaca ctacgtccgc acttattacc tgctccaact
      781 ctttgccgcc gcaggctatt tgtggacagg cgcggcgaaa ttacaaaacc tgcccgcctc
      841 cgcgcccctg cacctgatta ccctcggcgg catgatgggc ggcgtgatga tggtgtggct
      901 gaccgccgga ctgtggcaca gcggctttac caaactcgac taccccaaac tctgccgcat
      961 tgccgtcccc atccttttcg ccgccgccgt ctcgcgcgct ttcttgatga acgtgaaccc
     1021 gatatttttc attaccgttc ctgcgattct gaccgccgcc gtattcgtac tgtatctttt
     1081 cacgtttata ccgatatttc gggcgaatgc gtttacagac gatccggaat aaaaatgccg
     1141 tctgaaacgg ttttcagacg gcatcggatt gccaataatc aagcctcctg cggatcgaca
     1201 tccaccgacc atctgatttt gccgtcgcgg ttttgctgca acacctgcgc ccacaaactc
     1261 acggcacggt gcaaatcctg tcgggacgga gattcgagga agatttgcgc gcgttcgcgt
     1321 tcggcgaggc gcaccatcag catcggggcg gcgccgaact gtgaaacgct ttcgggcaaa
     1381 agcggggcga gggtttcttt ggcggcattg agaaactcca tcgcatcggc aacgcgcggc
     1441 gcgtcggcgc ggacggcggt ctggaaaccg aagggcggca tggcgaacat ttgccgctcg
     1501 ttcaattcgt tttcggcaaa cacggcgtag tcctgcgctt tgacggcggc gaagacggga
     1561 tgttcgggca gttgggtctg tatcaacacc ttgccgggtt tgtcggcgcg ccccgccctg
     1621 ccggacacct gcatcagctc ggcgaacagc ctttccgggg cgcgaaagtc cgcgctgtac
     1681 aggctgccgt cagcgttcaa cacgataacg aggttgagcc gcgcgaaatc atgccctttg
     1741 gcgagcatct gcgtgccgac caaaatgtcg attttgttgt cggcgatgcg gcggtacaaa
     1801 tccgcccagt cgtttttgtg cgcggtgctg tccctgtcaa cacggacgac ggctgccttg
     1861 ggcaggaagg tgcgcagggt ttcttcgacg cgctgcgtgc cgtggccgac ggcggtcagg
     1921 tcttggttgc cgcagtcggg gcatttgtac gggatgggtt cgcggtggtc gcagtggtgg
     1981 cagcgcagtt ggcgggcgcg ttggtgcagc accattttgg cggagcagtt cgggcagccg
     2041 aaggtataac cgcagtcgcc gcaaaacagc gcgggcgcga agccgcgacg gttgaggtac
     2101 accaacgaca tgccacctgc ttcaaagttc tgtttcaaaa gctgcaaggc ttgcggcgag
     2161 aagccgttgt caagtttcag acggcctacg ttgaggatgt ccacttgcgg cagttgcgcg
     2221 gcggtatggg cgcgttcggt cagttgcagc aggcggtacg cgccgctttg cgccttgtgc
     2281 cagctctcca agctgggggt ggcactgccc aatatgatcg ggcagccgcc ctgcttcgcc
     2341 cgccacaccg ccaaatcgcg ggcgtggtag cgcaattcgt tgtcctgttt gaacgagccg
     2401 tcgtgttcct catcgaccac aatcagcccg acatcatcca tcggcgtgaa caccgccagc
     2461 cgcgtgccga tgaccaattt cgcctgcccc aacatcgcgc gcaaataatc ctgcgtgcgc
     2521 ttgcctgccg ccatctgact gtgcaacacg gcggtcggca cgtcggcaaa acggttttcc
     2581 acccgcttca aaagctgcgg cgtgaggttg atttcgggca acagaaacaa cacctgccgc
     2641 ccctgcgcca acactttcgc catcgcatcg aaatacacct cggtcttgcc gctgccggtg
     2701 atgccgtaca gcaaaaacgg ctggaagctg ccgaaggccg tctgaatttc atcggaagcc
     2761 tgttgctggt cggcattcaa cacaaattca gagtgcgaag cctgcccgtg gtacgacctc
     2821 aatacaggtt tcgccgcttc cgttgtttca atccaaccct gctccgccca atcttcaatc
     2881 aatttcgccg cctgcgcgtt tacctgcttc aacgctgcca tcgtcattcc gcccgaaagc
     2941 agtgcgtccc acaaagccgc ttttttgttg aaccgagctg gtggcggcgt ttgcgccctg
     3001 cccgcttcgt tcaaagcata aaacaacggc ggctgcggca tttccaccgc gcgcgtttcc
     3061 ttcaaaccct gcggcagcgc ggcaaacacc gcctgcccag tcggatagtg gtaataacgc
     3121 gacgtaaatg ccaacaaatc acgccagctt tcaggcaacg gcttttcttc cacaaaggcc
     3181 gtctgaacac tcaaaatccg cgccatatcc atatcgggcg caatatccgt ttcccacacc
     3241 atcccgacca cggttttatt gcggaaaggc acaagcaccc gcgttcccgg aggaagcgga
     3301 tcggaatggg aataagtcaa aaggccgtct gaaagcggca cgtttacggc gatgcggtgg
     3361 taaatcatac aaacaagcaa atcatttttt gcgtatttta acagccggaa gctattttca
     3421 gacgataaat tgagcctagg cgcaatgcag gcgcaacaga gatttgatac aattccgctt
     3481 ttccaacacg cgcaatcatc tttatatgaa aaccaagtta atcaaaatct tgaccccctt
     3541 taccgtcctc ccgctgctgg cttgcgggca aacgcccgtt tccaatgcca acgccgaacc
     3601 cgccgtcaaa gccgagtccg ccggcaaatc cgttgccgcc tctttgaaag cgcgtttgga
     3661 aaaaacctat tccgcccaag atttgaaagt gttgagcgtc agcgaaacac cggtcaaagg
     3721 catttacgaa gtcgtcgtca gcggcaggca gattatctac accgatgccg aaggcggcta
     3781 tatgttcgtc ggcgaactca tcaacatcga cacgcgcaaa aacctgaccg aagaacgcgc
     3841 cgccgatttg aacaaaatcg acttcgcctc cctgcctttg gacaaagcca tcaaagaagt
     3901 gcgcggcaac ggcaagctga aagtcgccgt cttctccgac cccgattgtc cgttctgcaa
     3961 acgcttggaa cacgagtttg aaaaaatgac cgacgtgacg gtttacagct ttatgatgcc
     4021 cattgccggc ctgcaccccg atgccgcgcg caaggcgcaa atcttatggt gtcagcccga
     4081 ccgcgccaaa gcgtggacgg attggatgcg taaaggcaaa ttcccggtcg gcggcagcat
     4141 ctgcgacaat cccgtcgcgg aaaccacttc cttgggcgaa caattcggct tcaacggcac
     4201 gccgaccctc gtcttcccca acgggcgcag ccaaagcggc tacagcccga tgccccaact
     4261 ggaggaaatc atccgcaaaa atcaataatc catccgcaaa aaaggcttgg aaaattccaa
     4321 gccttttttg cgggaaatgc cgcctgaaaa ccaatccgcc ctgaaagtta gcaagccaac
     4381 aaacacatcc taacccattg aatgattgaa aataataaat tgaacaatac gaagccccgc
     4441 cgccgttccc acgcgggcgg gaatctggaa tattgagtta ccgtgattta tccgaaacaa
     4501 gcaaagccga acgaaccgaa ttcccgtctt tacgggaatg acgggactcg agcgttgtgt
     4561 gcaatcatcg aacccccgcc gccgtcattc ccacgcaagt gggaatctgg aacgtagaat
     4621 ctaagaaacc gtttttatcc aataagtttc tgctccaaaa tgaccggtcc tgcagcttca
     4681 gacggcatct ttgtccaacc tcaatcctgt gccaatgcgt ctatcggatt gagtttggct
     4741 gctttattgg caggcataaa gccgaacgcg atgccgattc cggtcgaaca ggcgaccgcg
     4801 ccgatgacgg acatggcgga aatgtccatc gggaagtcgg ttacaaaatg attgaacacg
     4861 aggctgacgg cggcggacaa acccacgccg accaaaccgc cgatgacgca gattaacacc
     4921 gcctcaatca aaaactgctg caaaatattg ccgcgccgcg cgccgattgc catccgtatg
     4981 ccgatttctt tggtgcgctc ggtaacggac accagcatga tgttcatcac gccgatgccg
     5041 ccgactacca atgaaatcag ggcgatggag gaaatcagca gcttcatcgt accggtggtg
     5101 ctttcgacta tctgcctgat gctgtcgctg ttgttcatga agaaatcttc cgtgccgtgc
     5161 cgcgctttga gcagatcggt cagccctttt tcggcaacct gggtattggc attgtctttg
     5221 attttgacgg tgatggagtt ggtgtggctc tcgcctgtga tttggtgcat caccgtcgta
     5281 tagggcgacc aaagcatcag cacgtcggaa ttgccgaaag cgttttcgtc ttttttcatc
     5341 acgccgatga cggtcaaggg gcgtttcctg aacaaaatgg ttttacccaa cggatccgag
     5401 tccgcaaaga gtttgtcttt gacattttgg tcgatgacga cgacctgcgc gtcttctttc
     5461 acatcgtttt cgtcaaacag ccgccccgtt tccagcttca gtccgcgcac gtcgaaatat
     5521 tgttcgccca cgccgtaaag cgaggcggtc aggtcggtgt tgcggtaagt cagcgtgccg
     5581 ccgctcgaag tcatgggcgt ggcggaagca acgtagcttt gtttggcgat gatttttgcg
     5641 tcgtctatgg tcagggtttt aatcctgccg ctgcgcctgt cgccgaagcc gcgccccggg
     5701 aagatgctga tggtgttcgt ccctatcgaa ctgatgtctt caaggatttt tttctgcgaa
     5761 ccattgccca atgcgacgac ggaaaccacc gacgcgatac cgatgatgat gccgagcatc
     5821 gtcagaagcg aacgcatttt gtgcgccaat actgcttgca ccgacattct gaaggcttcg
     5881 acaaactggt cgtaataaaa cgaccacgaa gctttttccc gaatcctccc gacattgctt
     5941 gcggggattt cgggattttt cgaggtgtcg gaaatgattt cgccgtcccg gatttcgatg
     6001 acgcggttgg cattggcggc gatgtcgggg tcgtgcgtga ccataatgac ggtatgcccg
     6061 gcttcgtgca gcctgcggat gatttccatc acgtttttgc cgctggcggt atcgagcgcg
     6121 ccggtcggct cgtcggcgaa gatgatttct ccgccgttca tcagggcgcg ggcgatggag
     6181 acgcgctgct gctgtccgcc cgagagttcg ccgggcttgt tgccctcttt gcttgccaaa
     6241 cccaaatcct gcaagagttt gtccgcccgc gcggaacgct ctttgccgcc cgcgcccata
     6301 tagacggctg gcagcgcgac gttgtccctt gcggtcagcg agcttaagag gttgtagcgt
     6361 tggaagatga aaccgaagcg ttcgcggcgc aatgccgcca gctcgtcagg ctgcattttg
     6421 gcagtttcga tgccgtcgat tcggtacgaa ccggaaccgg cggtatccaa acagccgagt
     6481 atgttcatca gcgtggactt gcccgaaccg gactgcccga tgatggcgac aaaatcgccc
     6541 ttctctatcg acaggctgat gtctttcaaa atatggacgc ggttctcgcc gctgccgaaa
     6601 tagcggttga tgtttttaca ttcgatcaag ctcataatat tccgtaaatc aataacaaat
     6661 gccgtctgaa accgtttccg tgttcagacg gcatattcgt ttatcggcgc ggcgggccgc
     6721 ctagggcgcg ttcgccgctt tcctgttgct cggcggcggt tatttcggag atgaccactt
     6781 tgtccccctc tttcaacccg ctttttactt cggtattcat actgtctctc ataccggtcc
     6841 ggatttcgcg ttccgccgcc ttgccgtccg cacccaacac gcgcacaaac gccttgccgc
     6901 cgcgattttt cacggtcagc gacggaataa tcagcacatt tttcacgccg tcgatttcaa
     6961 ccgtattctg cgtcgtcatc cccgtggaga gtttgccgtc cggattcggc acaaacgaac
     7021 gggcataata gtagaccgca ttggaagccg tatccgtact gctgttgtag ccgcccgacg
     7081 acatcgtggt cagcccgggg tcgacgctgt cgagcttcgc cttaatcggc gtatccggtt
     7141 cggacaaaat cgtaaacgaa atatcctgcc ccgccttcac cttggtaata tcgccctcgg
     7201 caatctgcat tttgttcaac atcatatcca gattcgccaa ttggacaatc gtcggcgtag
     7261 actgcgccgc gttcacagtc tgcccctctt ccacgagaat cgccaccacc gtgccgtcca
     7321 tcgttgcggt aatgcgcgtg tagcccaatt ccgactcggc ggtattgatg gaaattttgc
     7381 tctgtctgat taaagccttc agctcggcaa cattggcttt ggcggcggca aacgcatcct
     7441 gcgcgctttc caaatcctct ttggaagtcg cgttttcctt ccataacgcc gcctgacgct
     7501 tatatttctt ctccgcgctg cccaatgcaa tctgtgccga caccagcttc gcctgatacg
     7561 tttccaactt ggatttttcc gtattgagcg tattggtctg cgaggtcgaa ttgatttccg
     7621 caatcaaatc gcccttttta acctgttgcc cgagtttgac ataaagtttc ttaatctgcc
     7681 ccgatgcctg cgcgccgacc gataccaggt tggacggcga aatctcccct gttgcagaaa
     7741 ccgtccggct gatgtcgccg cgcctgaccg tttccgtaat ataagcagcc tgcggctcgg
     7801 gcttcagata agaccatccg ccccaaaccg ctgccgccgc gactgccgca acagccgccc
     7861 atttcatcat ttttgccatg ttttcaattc tctgtatgcg tcaatcccat aaatcggaag
     7921 cggattctac cgcaagtccc tgccccatcc aaggcggcaa actgccgaca ataaaaataa
     7981 ccatttgttt taaatggctt atacaatctg ctttcccgaa aatttcaatc cgtttacata
     8041 ttccgccatt tccgatacgg cgttataatg ccgtcgaccg acaaacaaat ggaaacacat
     8101 tgctatgaaa aacgttcaaa aaggctttac gctgctcgag ctgatgattg ccgtcgccat
     8161 cctcggcatc ctgacgctca tcacctaccc ttcctataaa acctacatcc ggcgcgtccg
     8221 cctgtcggaa gtcaaatcca ccctgctgat gaacgcgcaa aacttggagc gttactaccg
     8281 ccaaaaaggg acgtttgaaa actacgacca aaccaaactg aaacaaaaca aatatttcaa
     8341 gattacctta agcaaaagcc ccgaccactt cacccttcag gccgaccccg atccgatgac
     8401 caacgaaggc gaaacctgca tcgttacgct caacgacggc ggcacgcttt ccgccgccgg
     8461 caacggacag tcctgcccgg acttcgactg accctccgcc gaacccgcaa acccgtaaaa
     8521 atgccgtctg aagctgtgtt cagacggcat ttttacgggt ttgcccatcc ggtttttcaa
     8581 acttgaaacc tgccgccccg tcatccggca accgccccga tgccgcgcct ttctaggctg
     8641 ttataatata acatataatc tatatttttc aacaacatac cacttgacct gtgagatgcg
     8701 gaagcgtatg cttaccctta tatcggacaa cattccgttt acaggttttt ttgccgtaac
     8761 taaggagcgc aatcatgaaa atgcaggcag ttgttgtgaa taaaaatgta gcgggcgatg
     8821 tggaagtagt cgaacgcgag gttcgcccgt tggaatacgg cgaggcattg gtcgaagtcg
     8881 aatattgcgg cgtgtgccac accgacctgc acgttgcggc aggcgactac ggcgaaaaac
     8941 cgggccgcgt gttgggacac gagggcatcg gtttggttaa agaagttgcc gacggtgtga
     9001 aaaatctgaa agtcggcgac cgcgtcagca tcgcttggct gttccaaagc tgcggctctt
     9061 gcgaatactg caataccggc cgcgaaaccc tgtgccgttc cgtattgaac gcgggctaca
     9121 ccgccgacgg cggtatggcg acccactgta tcgtgagtgc cgattacgcg gtcaaagtcc
     9181 ctgaaggttt ggatcctgcg caagcttcca gcattacttg tgccggtgta accacttata
     9241 aagccattaa agtttccggc gttcgtccgg gacagtggat tgccatctac ggcgcgggcg
     9301 gtttgggcaa cttgggggtc caatacgcga aaaaagtatt cggcgcgcac gttgtcgcca
     9361 tcgacatcaa cgacgacaaa ctggcgtttg ccaaagaaac cggcgcggat ttggttgtca
     9421 acgccgccaa agaagacgct gccaaagtga ttcaggaaaa aaccggcggc gcacacgctg
     9481 cggtcgtaac cgccgtatct gctgccgcat tcaactctgc cgtgaattgc gtccgcgcgg
     9541 gcggacgtgt ggttgccatc gggctgccgc cggaatcgat ggatttgtcc atcccgcgtt
     9601 tggttttgga cggcatcgaa gtggtcggct ctttggtcgg cacgcgcaaa gatttggaag
     9661 aagccttcca attcggcgcg gaaggtttgg ttgtgccgaa agtccaactg cgtgctttgg
     9721 atgaagcacc cgccattttc caagaaatgc gcgaaggcaa aatcaccggc cgtatggtga
     9781 tcgatatgaa aaaagaatgc ggctgcggcc atcaccactg atttgacgtg gcagtacaca
     9841 tcgaaatgcc gtctgaacgc tgttcagacg gcatttttta tggatttgga tttgatttta
     9901 atctgttctg tttgaaatac cgtctgaaaa cccatatcgc aacacttcat taaaaacggc
     9961 aagattcagc cgttctgcaa cccgttcaaa cggcttccgc catttccaac gcctgtttga
    10021 tgtccacggc gacgacgcgc gagacacctt tttcctgcat ggttacgccg accagctgct
    10081 ccgccatttc catcgtcagg cggttgtggg agatgtagag gaactgggtt tgcgccgaca
    10141 tttctttgac cagcctgcag aaacgcgagg tgttggcgtc gtccagcggg gcatcgactt
    10201 cgtccaaaag gcagaacgga gcggggttga ggctgaacag agcgaacacg aggctcatgg
    10261 cggtgagggc tttttcgccg ccggagagga ggtggatggt gctgtttttc ttgccgggcg
    10321 gacgcgccat aatggacaca ccggcggtca gtaggtcgtc gcctatcatt ttgagagtgg
    10381 cttcgccgcc gccgaacagg gtcgggaaga aggtttggac tttgctgttg acggcatcga
    10441 aggtttcttt gaaacgcgct ttggttttgt cgtcgatttg ggcgatggct tcttccaaaa
    10501 gggtgatggc tgcctgcacg tcttcgcttt ggctgcggta gtagccgtcg cgttcgcgcg
    10561 cttcttcgag ttcttgcagg gcggcgaggt tgaccgcgcc gagtgcttcg atttgttgcg
    10621 aaaggctgcc gatgctgctg ttcaatactt tcggcgattc tttcgccaac gcttcgagcg
    10681 cgtccaaatc ggcggcgcgt tcggtcaggt tttgatggta gcgtttggcg ttgatcaggg
    10741 cttcctgctg ctgcaacaag gcggtttggg tggcggcctg aagctgcggc agcttggttt
    10801 gcagggtttg cactttagcg tattgctccc tgccctgttc ctgaatctgc gcgagtttct
    10861 cttgcacaac aatatattct tcgtccaagg tctgtacggc ttcgcttaat tcttcaagct
    10921 tgatgtgctg ctcgtcgttt tggaactcgg tttcataggc gagggcaagc tcttgctggc
    10981 gttcctgcca gtcgagggtt tgctgttcaa gctgggcgat ttgctgccgg tagttttgtt
    11041 tttgctggtt gagtttgtgg acggcgactt cggcaagccc gtattggcgg ttggcttcca
    11101 acagggcaag ctgcgcctgt ttcagacggc cttgctgctc ttggcggctg tgcgcggtgg
    11161 tttgctgctg gtgttcgagt tcggcggcgg cttcctgcaa ggtaacgatg tcgtctgaaa
    11221 gcccgtcgga cgtgtgttgc aacacggtct gttcttccgc caactgcgcc agttcgcgct
    11281 cgatgtgttc gcggcggatt tgcccttggt tggtacgcgc taagagttcg gcggcgcgtt
    11341 gctgtgcttg actgtattgg cgcgtgtgct gctgttgctg ctgcatcagg tttttatgtt
    11401 gcacttcaga cgagcgcacg gcagcttccg cctgtttgaa cgcggcttcg gcggcggaaa
    11461 gttcgggggc gaggttttcc agttcggacg cgatgccgtc gaggcgcgct ttttgggcaa
    11521 tcaggctttc ctgcgcgggt ttggcataga gcaggacgct gactttatcg acctgatgac
    11581 cttcgggcgt gagccagatt tggtgtgcgc ccaaatcgtt ttgatgcgcg agggcatagc
    11641 tcaaatcggg cgcgcacaat acgccgtcga gccagtagtg caatgccgtc tgaaacggcg
    11701 gctgcgcttg gatttggttc agcaatgcct gtacgggcag ggattttttg atgccgcctg
    11761 agaggtcgtc tgaaagccat gccgcctgcc cctgcggcaa aggctcgggc ggcacgaaac
    11821 cttgcggcac ggcgcgggcg tgcaggcgtt cggcaagaat gacggacaag gcgtgctgcc
    11881 actcggcggg cgcggtgatg tgttgccaca gttgcggcgc ggcggcgtgg tcggttgcct
    11941 gccagaaatc ggcggcttcc tgctgttgcg acaggatttg cgacaacgcc tgctgctgcg
    12001 cctgcaaggt gatgtgttgc tgcttcaggc tttggaagcg gtttgaggcc gtctgaaacg
    12061 cctcgcgggc ggcgtgtaag gcttcttcgg cggcaatgat ttgttcttcg taatgctctt
    12121 gctgactttg caacaaggcg gctgcttcct gcgcggcggc ggtttcggct tcgtcgggca
    12181 ggtttaaggc ttggttttcc tgtttcagac gacctttgcg ctcttcgtgc ttggcaatcg
    12241 tttgttcggc atgggcaagc tgctgctgct tcaacgccag ttcgcggcgg atgcggtttg
    12301 cctcgtcctg ctgggtttgg aaggcggcgt tgagcgtggc ttgggcttct tccaattcgg
    12361 gcagacgctc ctcgtgttcg gcaacctgca tcgcccattc cgccagctcg gtttgttttt
    12421 cttcgacctg caactcgttt tcttcaagct gcacgcggat ttgctgctgc tcttgatgaa
    12481 tgcgttgtaa ctgcgcctgc gctgcctgct tgtcgcgttc gatgcgttgg tgcaggtttt
    12541 gctgatggcg gatttgttct tccaaacggg caatctgctc gcgcaacacg ccgcgcttgt
    12601 tgctcaattc atgcactgcc tgctgctgcg actgttcggc agtctgcaag gcgtgtactt
    12661 cgtcgtttaa cgcctgaacc tgcgcggcgg tttcgtcctg ctgcgcctgc aaagattgat
    12721 gctgcgcggt cgccttatcg gcggcggcaa gcgattgccg ccattgggcg taatcgagca
    12781 aatcctgttg ctgattcagc tgcgcggtca gggatttgta gcgttcggcg gtttccgctt
    12841 gtttttccag cttttccacc tgacgcgcca actcgttctg caaatcgccc aaacgctgca
    12901 aatgctcgcg cgtgtctttc agacgacctt ccgtctcctt gcggcgttcc ttatatttgg
    12961 acacgcccgc cgcctcctcg atataggcgc gcaactcctc cggccgcgct tcgatgatgc
    13021 gcgaaatcat cccctgctcg ataacggcat aaccgcgcgc gcccacgccc gtacccagaa
    13081 acaaatcggt aatgtcgcgg cggcgcacgg tctgattgtt gatgaaataa gtcgattcgc
    13141 cctgccgcgt cagctgccgc ttgatgctca cctcggcata ctgcccccac gccccctgca
    13201 aactgtggtc gctgttgtca aacaccagct ccaccgaagc cctcggcgca ggacggcgcg
    13261 tcgccgcacc gttaaaaatc acgtcctgca tactctcgcc acgaagctgc ttcgccgaag
    13321 cctcgcccaa cacccagcgc accgcgtcaa tcacattcga cttgccgcag ccgttgggcc
    13381 cgataaccgc gacaagctgc cccggcacat gaatcgtggt cgggtcggta aaagatttga
    13441 agccggagag tttgatatgg gtcaggcgca taatggtcgg aaaaaataaa aaagaagcgt
    13501 attttaacgg aaatcccgcc gcaccacccc atatcttgcc ggcaaaacct taccgcatcc
    13561 cgcaccctcg atgccgtctg aagccttcag acggcatttt taacgcgccc gaaccccggt
    13621 tccgccaatg cccgatgctt ggcgcggcat ttgccgtatt gccccgccgc cgggcgcgtt
    13681 ataatccgcc ccgacatccg cccgattgga aacctgccta tgaaacccgc ctatttcatt
    13741 tccgacctgc atttgagcga aaagcagccc gaactgaccg cgctgctgct gcgtttttta
    13801 cgttcttccg ccgccgggca ggcgcgggcg atttacattt tgggcgattt gtttgatttt
    13861 tgggtgggcg atgacgaagt ttccgagttg aatacttcgg ttgcgcgtga aatcaggaaa
    13921 ttgtccgaca aaggcgttgc cgtgttcttc gtcaggggca accgcgactt cctgatcggt
    13981 caggattttt gccggcaggc gggcatgacg ctgctgccgg attactcggt tttggacttg
    14041 ttcggctgca aaaccctcat ctgccacggc gacactctgt gtacagatga cagggcatac
    14101 caacgtttcc gcaaaatcgt gcatcggaag cggctgcaaa aactgttcct aatgctgccc
    14161 ctgaagtggc gcacgcgcct tgccaccaaa atcaggcgtg tcagcaaaat ggaaaaacag
    14221 gtcaagcccg ccgatattat ggatgtcaat gccgccttta ccgcgcggca ggtacgcgcc
    14281 ttcggtgcgg aaaggctgat acacggacac acccaccgcg agcatatcca tcacgaaaac
    14341 ggctttaccc gcatcgtttt gggcgactgg cataacgact atgcttcaat cctccgcgtg
    14401 gacggggacg gcgcggtatt cgtgccgctg gaaaaatact gaaaatgccg tctgaagcct
    14461 ttcgggcggc tttttttaca cgcccctttc ccacactccc tccgccgcat catcactatc
    14521 cttaacccgc attaatagcc attatcgata aaatgcttga ccgattacat aagattacgt
    14581 aaagtgtgca aacgcataac aattggtctt accaatttat cttttaacca aaatatcggg
    14641 tgtacgggat ttgaacgtct ttccaaacct cgacgcgcct tatgatgcaa atgcctaaaa
    14701 aggattcgtt atggcacttt tcctcagcat attccccatc gtcctgctga tttggctgat
    14761 ggtgaaaaaa aacagtatgc cctcctacgt cgcgctgccg attaccgcag tgctgattta
    14821 cgccatcaaa cttttctact tcgacgatgc gggcatgctg ctcaacgcca ccgccgcttc
    14881 cggcctcgtc aaaacgctca cgccgattac cgtgattttc ggcgcgatta tgttcaaccg
    14941 tatgatggaa accacgggct gcatcgatgt catccgcaaa tggctggcga ccatcagccc
    15001 gaaccccgta gcgcaactga tgattatcgg ctgggctttt gcctttatga ttgaaggcgc
    15061 atccggcttc ggtacgcctg ccgcgattgc cgcgccgatt ctgatgagct tgggcttcaa
    15121 cccgttgaaa gtggcgattt tcactttggt gatgaactcc gtccccgtct ctttcggcgc
    15181 ggtcggtacg ccgacctggt tcggtttcgc accgctgaac ctgagtgccg aagacatcct
    15241 cgccatcggc aggcagaccg gcgtaatgca cttcttcgca ggtttcgtca tccccgtcat
    15301 cggcttgggc ttcatcgtac cttggtctga aatccgcaaa aacttgggct tcgtcgccat
    15361 tgccgtcttc tcctgcacca ttccttatgt cgcattggcg atggtcaacg aagaattccc
    15421 gtcgctcgtc gccggcgcga tcggcctgat ggtgtccgta ttcgccgcca accaaggctg
    15481 gggcttgagc aaagaccacg ccaaagaccc gaatgccgaa aaagtgccgt tcgcccaagt
    15541 cgccaaagca ctcgcccctt tgggtatgct gatcggcatg ctggtggtta cgcgcatcaa
    15601 acagctcggc atcaaaggca ttttgaccag caaagaagaa tggttcagct tccaactgcc
    15661 gtttgatttg tccaaaatca ccgtcagcga ctccctgacg attaccttcg gcaatatttt
    15721 cggacaagat gtcagcgcgt cttaccaaac gctgtacgtc ccggcttgga ttccgtttgt
    15781 gctgaccgtt tggatttgca tcctgctgta taaaaccaaa ttcaaagatg cctggacgat
    15841 ttattccgta accttcaatc aaaccaaaaa accgctgctt gccctgatgg gcgcgctgat
    15901 tatggttcag ctgatgctgg tcggcggcga caattcgatg gtgaaaatca tcggtaagga
    15961 atttgccgca atggcgggcg aacactgggt ttatttctcg ccgtatctgg gcgcgatcgg
    16021 tgcgttcttc tccggttcca acaccgtgtc caacctgacc ttcggcccga ttcagcagca
    16081 aatcgccttg gataccggcc tgtccgttac cctgattctg gcgttgcagt ccgtcggcgg
    16141 cgcgatgggc aatatggtgt gcctcaacaa catcatcgcc gtatgtaccg tattggatgt
    16201 gaaaaattcc gaaggtgcga ttatcaagaa aaccgttatc ccgatggcga tttacggcgt
    16261 gattgccgtc gtcgcggcaa tgattttctt cctctaggcg aaacgccacc atccatagcg
    16321 gcagcacgcg gggaacggaa accgccgttc accgtctggg cggcgcgtcg ggcagaatcc
    16381 cggtgccgga acaagcccgc cccgccaaac aaatgccgtc tgaaaccgaa aaaggcttca
    16441 gacggcattt ttccgccgtt tgcgttcagg cgtaatgatg atgcggcatc ttgccgtcaa
    16501 ggcggtagcg cgtgccgcaa tacgggcagg caacgctgcc cgattctcct tcgcacaaag
    16561 gcaaaaacac tctcggatgc ccgttccact gctcgttgcc gggtcccgag caatacagcg
    16621 gcagattttc cggcaacacg gaaatttcct gcggattcag attgtccatt tgattttcct
    16681 ttgcgtggtt ggcgtgcgcc tattttacgc catcggcagg ctaaaggata ttttcggcgc
    16741 aaagccgcaa tccgctataa tccccacttt tcagacggca taccatgact gcgcttaccc
    16801 ttccggaaga catccgccaa caagagccat ccgccctgct ctataccctc gtttccgcct
    16861 acctcgaaca caccgcccaa accggcgacg aatccctctc ctgcctgtcc gacgaccagc
    16921 acacgctgac cgcattctgc tacctcgaca gccaagtcga agaaggcggc ttcgtgcaac
    16981 tcatcgcatc cggttacggc gaatatattt tccgcaaccc gcttgccgac agcctgcgcc
    17041 gctggaaaat caaagccgtg ccgaaagtct tggacaaagc caaagccctc tacgaacaac
    17101 acggcaaaac catcgaaacg ctcgccgacg gaggcgcaga catcccttcc ctgcgcaaac
    17161 agttccccga atttgaagaa tgggacggcg catactacga agccgccgaa caagacctgc
    17221 ccctgcttgc agaacacata cagtcaaact gggaaacctt cgcccatatc gggcaggcgt
    17281 gattgcgtct gtttccagcc cgtgtaaaac agcgtaaaat cggcaaaccc gaatgacctt
    17341 ccgtccccat caaggagcaa gctatgttct tcaagcacat cgaagccgcc cccgccgatc
    17401 cgattctcgg tttgggcgaa gcgttcaaag ccgaaacccg ccccgaaaaa gtcaacctcg
    17461 gcatcggcgt ttataaagac gcatccggcg cgacacccct cgtcaaagcc gtcaaagaag
    17521 ccgaaaaacg cctgttggaa agcgaaacca ccaaaaacta cctcaccatc gacggcgttg
    17581 ccgactacaa cgcgcaaacc caaatcctgc tgttcggcaa agaccacgaa atcatcgcca
    17641 gccgtcgcgc caaaacagcg caaagcctcg gcggtacggg cgcattgcgt attgcggccg
    17701 agtttgccaa acgccagttg aacgcgcaaa ccatctggat ttccaatccg acttggccca
    17761 accacaacgc catcgccaaa gcggtcggta tccaagacaa accttatcgt tactatgatg
    17821 ccgccaaaca cggtttggat tgggacggca tgattgagga cttgagccaa gcgcaaaaag
    17881 gcgacatcgt cctgctgcac ggctgctgcc acaatcctac cggcatcgac cctacgcccg
    17941 aacaatggga aactttggca aaactttctg ccgaaaaagg ctggttgccg ctgtttgact
    18001 ttgcctacca aggcttcggc aatggtttgg aagaagatgc ctacggcttg cgcgtgttct
    18061 tgaaacacaa tacagaattg ctgattgcca gctcttattc caaaaacttc ggtatgtaca
    18121 acgagcgcgt cggcgcgttc actttggtgg ccgaagatga agaaacagca gcccgcgccc
    18181 acagccaagt caaaaccatc atccgtacct tgtattccaa cccggcttca cacggtgcga
    18241 acaccattgc gctggtgttg aaaaatgatg atttgaaagc acaatggatt gccgaactcg
    18301 atgaaatgcg cggccgcatc aaagccatgc gccaaaaatt tgtcgggttg ctcaaagcca
    18361 aaggtgcaag ccaaaacttt gatttcatta tcaaacaaaa cggtatgttc tctttcagcg
    18421 gcttgactcc cgaacaagtc gaccgcctga aaaacgagtt tgccatttat gccgtccgct
    18481 ccggccgcat caacgtcgcc ggcattaccg acaacaacat cgattatctg tgcgaaagca
    18541 tcgtgaaagt actgtaaaaa gcacagccat atgaaaatgc cgtccgaacc ttaaccagtt
    18601 cagacggcat ctgttttttg ttaaaaaaca ataaaccata acaatttcaa acaagctaaa
    18661 aacatctata aaatcaacca aatatgatga actcaaataa aaaatacgaa tatcatcatt
    18721 ctcaaattca aaatttgaac gccacagtat tatccgaagc cacaaaaacc tttccgccgt
    18781 cattcccgcg caggcgggaa tccgggaatc caatgctgca agaattgatt agaaacaact
    18841 gaaaccgagc aaactggatt cccgcctgcg cgggaatgac gaaataaggc gagtgaaact
    18901 gatgaaacaa attgattaaa aacaataact tatattattt tatttaaatt ttcgataaaa
    18961 tcaattttcc gtattcaata ctgctccaat caattcccgc gcaccgtcgt ccgccaattt
    19021 cttagcgaca gcgcgtccga gcgcgtcggc atattcggca ggggcttgcg cgtccgcccg
    19081 caacacaacc gaaccgtcgg ggtgtccgac caagccgcgc aaggtcagca agccgttttc
    19141 ttccgtgcaa tatgcggcca aaggcacttg gcagcttccg cccaaagcgc gtgcgagggc
    19201 gcgttcggcg gtaacgcagg cattggtaac accgtggttc aagggtttca aaacttcata
    19261 caaatcttcg cggtgcgcgg caatttcgat acccaatgcg ccttgtccgg cggcaggcag
    19321 gctgtcggat tccgacaaaa tcatgcggat gcgtccgtcc aatttcagac gctgcaaacc
    19381 ggcggcagcc aagataattg cgtcgtattc gccgttatcg agtttggaca aacgggtttg
    19441 cacattgccg cgcaaaggtt tgataagcaa atgcggatag cgcgcacgca actgggcttc
    19501 gcggcgcagg ctggatgtgc cgacaaccgc gccttcgggc atttcttcca aacgcgtgta
    19561 ttggttggac acaaacgcgt caaacggatt ggcgcgttcg ccgatggcgg caagcgcgaa
    19621 accttcaggc aaatccatcg gcacgtcctt aatcgaatgc accgccaaat cggcgcgccc
    19681 gtcatataaa gcctgttcca actctttgac aaacaagcct ttaccgccga cttttgacaa
    19741 agttttgtcc aaaatctgat cgccgcgcgt ggtcatgccc aaaatctcga cttcgcaatc
    19801 gggatacaga gccttcagac ggccttggat atgctttgcc tgccacatag caagcaggct
    19861 ttcgcggctg gcgatgacga gttttttcgg gttcatgttc aatgcgttca aagaatggaa
    19921 acggcgtgaa gtctatcata ttttgaacgt ttcttcagac ggcaccgtca ggtctatgtt
    19981 atatttacac ccacaaaaca cgaaacccgc cacaatgaaa cgcaccaaaa aatacccttt
    20041 cttaacgttg cagcggcaac gtttccattt gaactttgaa aacgcctctt ccgccgccgg
    20101 catcccagcc gaacgcgatt tctaccgctg ggcgtggtct gccttgaaaa atgaataccg
    20161 ccgcgccgac atcagcctga ttcttctgga cgaagaagaa gcccgagcct acaaccgcga
    20221 ctaccgcggc aaagattacg ccaccaatgt attgagtttc gcgctcaacg aaggcgaaat
    20281 cctgccctgc caagtttcgg aaaaactgta cggcgatttg attatctgcc cgcaagtggt
    20341 tttgaaagaa gccgccgaac aaggcaaaac acccgagcag cattttgccc acctgaccat
    20401 acacggtact ttacacctga tgggctacga ccacatcgaa gacgacgaag ccgaaataat
    20461 ggaagccgaa gaaatccgcc tgatgcgggc ggcaggcttc cccaacccct accaagagga
    20521 ctgacattaa aatggacggc gcacaaccga aaacgaattt ttttgaacgc ctgattgccc
    20581 gactcgcccg cgaacccgat tccgccgaag acgtattaaa cctgcttcgg caggcgcacg
    20641 agcaggaagt ttttgatgcg gatacgcttt taagattgga aaaagtcctc gatttttccg
    20701 atttggaagt gcgcgacgcg atgattacgc gcagccgtat gaacgtttta aaagaaaacg
    20761 acagcatcga gcgcatcacc gcctacgtta tcgataccgc ccattcgcgc ttccccgtca
    20821 tcggcgaaga caaagacgaa gttttgggca ttttgcacgc caaagacctg ctcaaatata
    20881 tgtttaaccc cgagcagttc cacctcaaat ccattctccg ccccgccgtc ttcgtccccg
    20941 aaggcaaatc gctgaccgcc cttttaaaag agttccgcga acagcgcaac catatggcga
    21001 ttgtcatcga cgaatacggc ggcacatccg gcttggtcac ctttgaagac atcatcgagc
    21061 aaatcgtcgg cgaaatcgaa gacgagtttg acgaagacga tagcgccgac aatatccatg
    21121 ccgtttcttc cgaacgctgg cgcatccatg cagctaccga aatcgaagac atcaacacct
    21181 tcttcggcac ggaatacagc agcgaagaag ccgacaccat tggcggcctg gtcattcaag
    21241 agttgggaca tctgcccgtg cgcggcgaaa aagtccttat cggcggtttg cagttcaccg
    21301 tcgcacgcgc cgacaaccgc cgcctgcata cgctgatggc gacccgcgtg aagtaagcac
    21361 cgccgtttct gcacagttta ggatgacggt acgggcgttt tctgtttcaa tccgccccat
    21421 ccgccaaaca taaaaatgcc gtctgaaacc gttgcaagtt tcagacggca tttcgcttat
    21481 ccgcttattt tttgctcaaa gtcagccccg tccaaccgaa taacagggtc aaagccaaac
    21541 tcaaatagca gaaaaaggca tacggcagat attcccaaac cggcacgccc agcgcgtggc
    21601 tgatgaacac gccgcatacg ctccacggta cgagcgggtt aatcaccgtc cccgcatctt
    21661 ccagcgtccg cgacagattg cgcgaatgca gaccgagctt atcgtaaacg ggtttgaacg
    21721 tttcacccga caacaaaata ctcaaatatt gctcgccgat caggaaatta accccgaccg
    21781 aagtcatggc aacgctgaac gtcgcgcgtc cggcattcgt caagaaggta cggatggcct
    21841 ccaacaggga aggaatcaca ccgagcgcaa acaacagtcc gcccaaactc atcccgagaa
    21901 tcacgatggt ttgcgtgaaa aacatacttt ccaaaccgcc gcgcgaaatc agtttgacaa
    21961 catctttaaa cgcttcgcct tcgagtttgt agccgccgta aaaccacgca ccgagctgac
    22021 gcagatcggg cgtgctgtgc agatacgtta cagcaacggc aaccatgacg gtaaagagca
    22081 tggcgacgac ggcgttgatg cgcatcaatg ccaaaatgac caacagcgca aacggaatca
    22141 gcgaatagcc gtgcaccaat cccgtggctt caagctggct gcggaaggat tcgacgctgt
    22201 tcaaatcctg cgcggcgaca ttcggcaaaa gccaaagcat cagtgccgca ctaatgagcc
    22261 acgcggggat ggtggtgtac atcatatttt tgatgtgctc aaacaagtcg atgccgacga
    22321 tggacgcgga aatacccgtc gtatccgaaa gcggggacat tttgtcgcca aaaaatgcgc
    22381 ccgaaacaat cgcgcccgcc gtcatcgcca tatcggcctg aaacgccgcc gccatcccca
    22441 taaaggcaac gccgacagtg gcgcaggtgg tcaggctgct gccgatggac acgccgatga
    22501 cggaacacag cgcgaaggag gaaaaataaa aataagtcgg ggaaatcagt ccgaaaccgt
    22561 aatacatcag cgtcggaatc gcgccgctca tcatcagcgc gctgaccatc agcccgatga
    22621 agaaaaacag gtaaatcgcg cccataccct gattcaacgc gcctatcatg ccctgctgca
    22681 tatcgttgta tttcaaaccg cgcgccaagc cgtacaaaat cagcacgacg atggcggcaa
    22741 taatggacat atgcggcaac cactccaatg aaatgatggt atagcccatc gcggcaatca
    22801 gagcgacgac gacggcaagt gcctcaccgc gcggcatatc gagtaaggat ttgaaagcga
    22861 acatgattct ttgcccgatt tgatataaag ttaacggatt ctaacacacc ataccgaatc
    22921 cgcccgacgt tttcagacgg cattttagcg ttttacaaaa gccggacaga aacaaaacgg
    22981 caattttctc aatttaaatt aatttttttg aaatataatt ttttattttt ttgtttgttg
    23041 acatataaga cttttatgga ttgaagtatt aaaacctcgg cacacacgcc gcaaacaccg
    23101 gtacgcaaaa atgccgtctg aaagatgttc agacgacatt tctcaagaaa aagattattt
    23161 gtccgcccta tatccgtaca ccgcaaaaaa cacgatatac agataacaga tgacggaaac
    23221 gacaaacgaa gacatcaggg tgtaagtatc tgccacccag ccctgcacga caggaacgac
    23281 cgcaccgccg acaatcgcgg tacacagtac accggaagcc gcgttggtaa attttcccaa
    23341 tcctttggtt gccaaagaga aaatcgtcgg aaacataatc gagttgaaaa aaccgatggc
    23401 aagcagcgac cacatcgcca catccgcatt gccgctaccc gtcgccatcg cgacggcaag
    23461 cagtacgacc gcagccgatg cgttaaacgc caaataacgg ttgggcgcga atttcgccat
    23521 caccgccgaa ccgaggaaac gtccgaccat cgcgccgccc caatagaacg acaggtaatg
    23581 cgcggcagaa gcatgatcca gccctttcag ataacccaat acgttgacca tcaacgaacc
    23641 gatagacacc tccgcgccga catagcagaa aatgcctgcc gtaccgaaca cgagatgttt
    23701 gtattgccat acgctggttt tgccgtcgtg gttgtgcgcg ctttcctcgg cggcaatttt
    23761 gcgcgcgtcg ggcagccgga tcattttcac gaaaacggca aggataatca gcagccccgc
    23821 cagtcccaaa tagggaatct gtacggaaga aatctgttcc gccttgctga cggtttgggt
    23881 tgcgtccgcc agaatcagga acgcgccgat ttgcggcgca atggtcgtac ccaaagcgtt
    23941 aaacgcctga accagcgtca gtgttgccga ttccttgccg ggtttcgcca gcagggtaac
    24001 ataaggatta ccggcgacct gaagcagcgt tacgccggaa gccaaaataa acaacgcgcc
    24061 caaaaatacc gcgtaagaat ggctacccgc agcaggataa aacagcaggc atccgaccgc
    24121 cgtcagcaga aagccgccga taacgccgtt tttgtagccg actttgccga caaaagcccc
    24181 catcgggatg gacatcaccg cataggcggt aaagaaacag aattggatca gcatcgcctg
    24241 aacgtaagac aggtcgaaaa tttctttcaa atgagggata aggatgtcgt tcatgcaggt
    24301 aataaaaccc atcatgaaga acagcgtggt caagacgacc aatggggacg tatgattgtt
    24361 ttgtgattgc gcagacatga tttgcctttc gtggtgtcgg aatattaccc gtttcaagaa
    24421 aatagaaatt gacatttctt aacggcaatg caaatcatac tgatatttga acattttgca
    24481 aactttatcg gatgcaggca atgcgccgat ctgaaattca agcagcagaa attaatattt
    24541 tacagttgct tgcatagatt ttaatagatt ttaatagatt ttaaaaaatt ccggcggact
    24601 tgcaaaaacc gttcccaata aaatgaaaat cctccgccgt atccggaaaa ttcggaaatg
    24661 ccgcatcgtg gcatcaaaac cggagcggaa agaatgtcgt atgcccgaca aaaaaggggc
    24721 atacggctgt gccgtatttt ttgcaccgtc ctccgccctt cggcgggggc gtgaaaatat
    24781 agtggattaa caaaaatcag gacaaggcga cg
//
LOCUS       DAWIVB010000010        24298 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWIVB010000010 DAWIVB010000000
VERSION     DAWIVB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.010"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(287..2128)
                     /locus_tag="WIX60_000287"
     CDS             complement(287..2128)
                     /locus_tag="WIX60_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ5304616.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDIFGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            2219..2689
                     /locus_tag="WIX60_000288"
     CDS             2219..2689
                     /locus_tag="WIX60_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ5304617.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(2762..3574)
                     /gene="hpnC"
                     /locus_tag="WIX60_000289"
     CDS             complement(2762..3574)
                     /gene="hpnC"
                     /locus_tag="WIX60_000289"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ5304618.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LNGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(3661..4143)
                     /locus_tag="WIX60_000290"
     CDS             complement(3661..4143)
                     /locus_tag="WIX60_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ5304619.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            4616..4912
                     /locus_tag="WIX60_000291"
     CDS             4616..4912
                     /locus_tag="WIX60_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304620.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPAAEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            5022..6389
                     /locus_tag="WIX60_000292"
     CDS             5022..6389
                     /locus_tag="WIX60_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ5304621.1"
                     /translation="MLDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPLS
                     VKSVQTIMRFCHQHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFANPLDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINSTVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(6570..8609)
                     /gene="autB"
                     /locus_tag="WIX60_000293"
     CDS             complement(6570..8609)
                     /gene="autB"
                     /locus_tag="WIX60_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phase-variable autotransporter AutB"
                     /protein_id="HEZ5304622.1"
                     /translation="MYYETRSKQASKQKFKKSFIVSLFFSILYTSPLLAVDYVYDKTK
                     LTNDEITRLKKLRDKTSEYWKKETYLITEDNPKVPPFPALYPRTYQFENINNSKKISF
                     YDQEYTEGYLVGFARGLGVAKRNGDTEEQIRKYFKECFNSNTKIRDYSTCQAEKFGSH
                     PLIVKSHIFSLGPKIKNSHINSEILSVGNYTEWANQVIHHIENYVSFAAHLYSGLDPF
                     HYIEVTDNSHVIGQTISLDEFRLENSLWEPRWDSNVGKLKTTNADIRFNTKSESLLVK
                     EDYAGGARFRFAYDPKEAKNTALIFEKNVTGTSDIIFENPIDDLKSLDGHQIIKVNGT
                     ADKHAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDMAIYAQQAQAANTLFALRLNN
                     KNSDIFDRTLPRKGLWLRVIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQL
                     SIGLMGGQAEQRSTFHNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWMQYQ
                     RFRHRINTEDGTERFTSKGITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGV
                     NGKFSDSENAHVNLLGSRQLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVE
                     MDGERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(8975..9421)
                     /gene="smpB"
                     /locus_tag="WIX60_000294"
     CDS             complement(8975..9421)
                     /gene="smpB"
                     /locus_tag="WIX60_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ5304623.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(9476..10486)
                     /gene="waaF"
                     /locus_tag="WIX60_000295"
     CDS             complement(9476..10486)
                     /gene="waaF"
                     /locus_tag="WIX60_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ5304624.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENSFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(10617..10692)
                     /locus_tag="WIX60_000296"
     tRNA            complement(10617..10692)
                     /locus_tag="WIX60_000296"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10657..10659),aa:Asn,seq:gtt)
     gene            complement(10884..11636)
                     /locus_tag="WIX60_000297"
     CDS             complement(10884..11636)
                     /locus_tag="WIX60_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ5304625.1"
                     /translation="MITLPSLNNLPSKWDEIRHWLESRVFECGVMPHPNLTECEAKQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGSRGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALEHGEVQTALF"
     gene            11937..12752
                     /locus_tag="WIX60_000298"
     CDS             11937..12752
                     /locus_tag="WIX60_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylethanolamine-binding family protein"
                     /protein_id="HEZ5304626.1"
                     /translation="MTAYNESTYSSSKGIIYANPHPLRRTAGFFNRCLCRGRIHAAIR
                     QPVRRRRLHAKPAFERALRLLLFRRQCFARAVVEKSARRDKSFVLTVYDKDAPTGLGW
                     MHRVVADIPADVHRRNATSLQLSRCANIADRTGLDALGGRRHSRRCPPPQRGLAAIKP
                     LRQHRRRPVRSHIGGNQFADLPHQVDAFVHGKTDAVMLQPRQHAAKRGLRSIVRHFFI
                     RQYRSLIIKRRAHTPGIRQLYRIAHCLRRPVLCGEPFYGFLFESNPLRLIEAV"
     gene            12939..13049
                     /locus_tag="WIX60_000299"
                     /pseudo
     CDS             12939..13049
                     /locus_tag="WIX60_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(13423..14568)
                     /gene="dapE"
                     /locus_tag="WIX60_000300"
     CDS             complement(13423..14568)
                     /gene="dapE"
                     /locus_tag="WIX60_000300"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ5304627.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAKHPNHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAQELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(14681..15301)
                     /locus_tag="WIX60_000301"
     CDS             complement(14681..15301)
                     /locus_tag="WIX60_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ5304628.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(15335..15844)
                     /locus_tag="WIX60_000302"
     CDS             complement(15335..15844)
                     /locus_tag="WIX60_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ5304629.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(15939..16490)
                     /gene="azu"
                     /locus_tag="WIX60_000303"
     CDS             complement(15939..16490)
                     /gene="azu"
                     /locus_tag="WIX60_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ5304630.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(16771..17193)
                     /locus_tag="WIX60_000304"
     CDS             complement(16771..17193)
                     /locus_tag="WIX60_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ5304631.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            17324..20074
                     /gene="secA"
                     /locus_tag="WIX60_000305"
     CDS             17324..20074
                     /gene="secA"
                     /locus_tag="WIX60_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ5304632.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSKLLTQAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            20089..20241
                     /locus_tag="WIX60_000306"
     CDS             20089..20241
                     /locus_tag="WIX60_000306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304633.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            20219..21991
                     /locus_tag="WIX60_000307"
     CDS             20219..21991
                     /locus_tag="WIX60_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ5304634.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVHGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLTQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            22177..24105
                     /gene="rpoD"
                     /locus_tag="WIX60_000308"
     CDS             22177..24105
                     /gene="rpoD"
                     /locus_tag="WIX60_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ5304635.1"
                     /translation="MSRNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         6320 a         6754 c         5636 g         5588 t
ORIGIN      
        1 tgattaacta ttttttaatc atgttattat tttccataaa atacatgaca ttaagatgtt
       61 tttccacaaa agatacacac accggcaaac accggctgtg tttatctttt cttatgccta
      121 ttttttaatc atcgtatttt tatcttttaa tttcaatacg caaactaact tatacacacg
      181 gttttcacat ctttagactg cttccgtgtg tatagtggat attgccgttt tcctttctga
      241 caaaaatgcc gtctgagaac ttcagacggc atttgaaaca tcggaatcag cggttttgtt
      301 cataccactc gataaacttg tctgctttga caaaacccag cagcggctcg ctgcggctgc
      361 cgtcggagcg gacgacaaac acgcccggcg gcccgaacag accgtattct ttcaacaacg
      421 cctgatgttc gggcgtgttg gcggttacgt cgatttggaa aaagcgttcc atatcgactg
      481 cctgatgcac ttccggctga ttgagcgtgt aagccgccat ttctttgcag gaaatgcacc
      541 agtcggcata aaaatccaaa acgacgggtt tgtcgggatg ttctttcaac gccgtatcca
      601 tcgctgcctt cagcgcggca gtatcggcaa acattttgcc gtgttccgaa gatttgcctg
      661 cttcggctgg tggattgagg gtcaggaaat ggtgcagcgc ggtcgttttg ccgtttgcgc
      721 cctgccagcc gaaccacgcg ccgcctatca gcaatatacc gcccaatgcg aatgccacag
      781 ctttcggacg gcgtttctgc ctgcgtccgt tgaccagcag cataaaggca ggaaccagca
      841 tcagcagcgt gtacagcgcg acgacgagat aatagggcaa gtgcggcgtg gcgaggtaaa
      901 cggcgacggc tagcaggatg aagccgaatg cgtatttgac ggcattcatc caatcgcctg
      961 ccttaggcag gatatgcccg ccgaacgtgc cgatggcaat cagcggaacg ccggtgccca
     1021 acgccaaagt gtaaagtgcc aaaccgccta aaaccgcatc gcccgtctga ccgatgtagc
     1081 ccaaagcaaa tgccagcggc ggggcgacgc acggcccgac aatcagcgcg gacaatatgc
     1141 ccataataaa gacggaaacg attttaccgc ctgaaagcct gctgctttga ttctgaaaat
     1201 acgactgcac ggcgttggga agctggatgt tgaacagccc gaacatagac agtgccaaga
     1261 cgaccattaa agccgatgcc gccaatacca cccaagcctg ctgcaaccat acggtcagca
     1321 gtgcgcccgt cagtccggca acaatgccga ccagcgtata agtcagagcc aaaccctgaa
     1381 cataaacgac ggacagcaca aacgcccgcg ccttgcccgc ctttttgtcg ccgaccacaa
     1441 tactggaaac aatcggcaac aggggataca tacaggcggt aaaactcagg cccaaaccag
     1501 cgagaaaaaa cgccaaaaga ttggcgttga gcgtatccca agacagcttg aaacggctgt
     1561 cgccgccctc atcccccttc gggggcggca gcgccccgct gccgttttga gaggaaggct
     1621 gcaaaaagcg gtctttggcg gatgccggtt cgtcggtttg cggatggtaa gtgccgttgc
     1681 cgaaaatatc aaactcggta tccacgggcg gatagcacac gccggcttcg gcacagccct
     1741 gataggtcaa aaccaattta tacggttcgc cgacagcctt tgcataagga aaggcaacct
     1801 gcgcctcgtg atggtaaacc gtctgcctgc cgaaaaactc gtcttccttc tcttcgccct
     1861 tgctgaaaga aggctgtccc aacaaatccg ccggatcggt cttgccgacg attttcgcct
     1921 gatacatata gtatccgtcg gcaatcctga aacggacgtt cacaccgtcg tcggcaacgg
     1981 caagctccgg cacgaatgcc ttttccggcg gcagcagatc gttcgcatcc agcgcgaaag
     2041 ctcgtccgca caacatcaaa aatacggcga acaggcaaat cagttttttc ataatcgaat
     2101 ccgtttcaga caaataattt gtctgcatta taaatggtaa ggttgacggt gggatttaat
     2161 ttatgtaaaa cccgccatta tccgaaccta tttccataaa catcttatcg aacccgccat
     2221 gtacgatgtc aatacccacg atgtccgccg ctttttcgcc cgcgtgtggc agcagcggct
     2281 caatccgctg caactgagcg cactggaaca gaaagccctc cgcattgtcg aagcccatcc
     2341 cgaataccac cgttatctcg aacgcatcga agaccatctg gacaccgact ggctgcccga
     2401 aaacggcgaa agcaacccct tcctgcatat gtcgctgcat ctgtccgtcc aagaacaggc
     2461 gggcatagac cagccgcacg gcatacgcgc aatccacgac accctgtgcg ccaaacgcgg
     2521 ctggctggaa gccgaacacg aaatgatgga ggcactggcg gaaacactgt ggacggcgca
     2581 acgctacggc accggtttgg atgtcaattt ctacatgacc cgactgcgca aactcatcgg
     2641 cttgggtgca gaagatcaag ccagattgaa cccgcatgaa atcgcctgac cataccaacc
     2701 gcctgcaaaa tgccgtctga agcggaacaa cccctttcag acggcattca ttttccccca
     2761 atcatttcca caacgccttt ttcagcataa tcaaccaatc cttcttatcc aaaacggggc
     2821 gttgtgcaaa cacatcgtat cggcacgcgt ccagtttctg caaaatcaac tgcgccccca
     2881 acacaatcat acggagttcc aaaccgatac gcccattcag ttcccttgcc aaaggcgaac
     2941 ccgccttcag catacggaac gcacgccgac actcatacgc catcagccgc tgaaacgccg
     3001 catccgcccg tcctgccgcg atctgttcct cagaaacacc gaatttcaac aaatcgtcct
     3061 gcggaatata aaccctgcct ttttgccaat ccacagccac atcctgccaa aaattcacca
     3121 gttgcaaagc cgtacagatg ccgtcgcttt gcgccacgca caccgcatcc gttttcccgt
     3181 acaaagccag cataatgcgt ccgacagggt tggcggaacg ccgacaataa tcggccagct
     3241 cgccgaaatt tccatacctt gttttaacca catcctgaga aaatgcagaa agcaaatcat
     3301 aaaacggctg caaatccaaa ccgaacggca caaccgcctc ggcatccaat cgtgcaatca
     3361 aaggatgcgc cgaccggccg cccgatgcca acacgtccaa ctcgcgctgc aaaccctcca
     3421 accccgccaa cctggcttca gacggcatac tgccctcgtc cgccatatcg tccgccgtcc
     3481 gtgcaaacgc gtacaccgcg tgaaccggct tcctcaacct gcgcggcaaa atcagcgaac
     3541 cgacgggaaa attctcataa tgcccaaccg acataccttc tccatccatc aaacaaaatg
     3601 ccgtctgaaa cggaacaaac ccttttcaga cggcatcaga tacctccaag ctgccggcaa
     3661 tcagtggtgg tgatgaccgt gcgggccgtg gacatgaccg tgtgcgattt cctcatcgga
     3721 tgcatcgcgc acgctttcaa ctgtagcctt aaagcggatt ttcatgcctg ccaaaggatg
     3781 gttgccgtcc accaccgcct tgccgtcggc aacatcggtt acacgataga cgacaacatc
     3841 gccggtttca ggatcgtcgg cttcaaacat catgccgact tcgacttcaa cagggaacac
     3901 gcccgcatct tcgatacgga ccaactccgg atcctgctcg ccgaacgcat cgtcgggcga
     3961 cagcgccaca tcgaccgtat cgccggcatc cttaccgtgc aacgcctctt ccaccaaagg
     4021 gaaaatgccg tcgtaaccgc cgtgcagata cgcaatcggt tcttcggttt tgtccaaaag
     4081 ctgattgttg gcatcataca tctcataatg cagcgaaacc acggaatttt tcacgatagc
     4141 catatttgtc ctttcaggaa cagcagatta attacaggcg cattctaaca caaccgccgc
     4201 gccggccgat taccgttaac ctgttcataa actgtacagc acatatttca atgtaaatct
     4261 ttgttatttt attgcggtgt aactttttta caacattctt aaaaccattc cgacctgtct
     4321 gccgactttc ccaatccgcc ttaataaatc atacaagata ctgaaattat attaatctct
     4381 ataatattta tccctatcga atttttaaca gcaaaaccgt tttacaggat ttatcaatcc
     4441 gcccgccaga aaacttttca ttcaaacctt tttcccatct gtacgacatt gcaatccctt
     4501 attccatagt gcataattac gcaaattcag cgatgaattt ccaacccggt ttgtagtatg
     4561 gtcgataaag acctatttgt ttcaataatt taaattggtt ctaaaggtta ctaaaatgaa
     4621 aaaatccctg tttgccgctg ctttgttgtc tttggttctg gcagcctgcg gcggtgaaaa
     4681 agccgctgaa gctcccgctg ctgaagcacc tgccgccgaa gctcccgcta ctgaagcacc
     4741 tgccgccgaa gctcccgctg ctgaagcacc tgccgccgaa gctcctgctg ctgaagctgc
     4801 cgctaccgaa gcacctgccg ctgaagctgc cgctaccgaa gcacctgccg ctgaagctgc
     4861 cgctaccgaa gcacctgccg ctgaagctcc tgctgccgaa gctgcaaaat aagcattttc
     4921 cgcttgcaaa aaagcaggat acgttcagta tcctgctttt ttgatttttc agacggcatc
     4981 agattccctt cctcaatctt ctccctaccc ttccgacaaa catgcttgac cttcataccg
     5041 aattttcccg actcctaccg gcagatgaaa ttgccgaacc ttctccgacg cttttaaaag
     5101 accagcgcaa ccgctttacg tctgcaccag acatcatttt gcagccgctc agcgttaaaa
     5161 gcgtgcaaac cattatgcgt ttctgccacc aacaccgtat tccggttacg ccgcaaggcg
     5221 gcaatactgg tttgtgcggc gcggcagtat cggaaaacgg cgtattgctg aacctttcca
     5281 aactcaaccg catccgcagc atcaatttgt cagacaactg cataaccgtc gaagcaggtt
     5341 ccgtactcca aaccgtccaa caggcagccg aagcctcaaa caggctgttc ccactcagtc
     5401 tcgccagcga aggctcgtgc caaatcggcg gcaacatcgc ctgcaatgcc ggaggtttga
     5461 acgtattgcg ttacggcacg atgcgcgacc tggttatcgg tttggaagtc gtcctcccca
     5521 acggcgaact ggtttcccat ctccatcccc tgcataaaaa caccaccggc tacgacctgc
     5581 gccatctgtt tatcggtagc gaaggtacat tgggcattat cactgccgcc acgctcaagc
     5641 tgtttgccaa ccccttagac aaagcaaccg catgggtcgg catacccgac atcgaatccg
     5701 ccgtccgcct gctgaccgaa acccaagcac actttgccga acgcctatgc agttttgagc
     5761 tgatcggccg ttttgccgcc gaattgtctt ccgaattcag caaactcccc ctgccgacac
     5821 attcagaatg gcatatttta cttgagttga ccgactcatt acccgacagc aatcttgatg
     5881 atcggcttgt cgaatttctt tataaaaaag gctttaccga cagcgtgttg gcgcaaagcg
     5941 aacaagaacg tatccatatg tgggcgttgc gcgaaaacat ctccgcatcg caacgcaaac
     6001 tgggcaccag catcaaacac gatattgccg ttcctatcgg gcgcgttgcc gactttgtcc
     6061 gccggtgcgc caaagatttg gaacagaatt tcaaaggcat acaaatcgtc tgcttcggac
     6121 atctgggcga cggcagcctg cactacaata ctttcctgcc cgaaatcctc agcaatgaag
     6181 tctatcgtta cgaaaacgac atcaacagca cagtctatcg caacgtcctt gcctgcaacg
     6241 gcacgattgc cgccgaacac ggcataggta tcatcaaaaa acagtggctg gacaaagtac
     6301 gcacgcctgc cgaaatcgcc ctgatgaaaa gcatcaaaca acaccttgat ccatataaca
     6361 ttatgaatcc gggcaaactg cttccgtaac cggcatttct gatttgcata cacaacaaag
     6421 aaagggacaa tagatccgat tgtcggttta gcgcgagctc gtgagtgcgg ttaaaaattg
     6481 gtggaaatta cacgaaaaat gaccgcactt ttaaaataaa aaaatcggca gtgaatttcc
     6541 ctgccgattt tattttgtta caacttaact taaaacgtcc actgtaaatt caacgcacct
     6601 tgtttagctt gatgatgttt gcctgtttgg cggttgaatg tggcttgtaa ggttaagtga
     6661 gatttgattt tcactgctac acctaattgg ctctcaattg ccgtcttatt gtttatcact
     6721 cgacgctctc cgtccatttc cacaccgaaa ggtttgttgt ggtaaagcgc gttcacagcg
     6781 gcgaaaggtt caatagcgat atttttatag agtgaaaatt gagctttagc ttgaacgcca
     6841 acccgagttt gtaattggcg ggagccaagt aaattcacgt gggcattttc gctatcgctg
     6901 aattttccgt ttacccccaa ataagtcaat tgtgcctgtg gttgtaggta aacacgaagg
     6961 ctgttgccct ttttagtgaa gtgttccgcc aataacgcat tgtaacctgc ttcaattgag
     7021 gcagtaatac cttttgaagt aaaacgttct gtaccatctt cagtgttgat acggtggcgg
     7081 aagcgttgat attgcatcca gctatccgca tacgcacctg tctgtttgtc ctgaagttgg
     7141 tgccaagtgg cgtaaacgcc tgcaccaaag cctttcacat ttcccgttgt aagattgtct
     7201 gtatctgggt tgtggaaagt gctacgttgt tctgcttgtc cgcccattaa gccaatagaa
     7261 agttgattac tttcgttttg ccatgtgaat acttcgccgc cgagttgcac acctttacga
     7321 tagccttcta caggtgctgt tttgccttgc acccattggt tggaatgtcc gtcaatcaca
     7381 cgcaaccaca agcctttgcg tggtaaagtg cggtcgaaaa tatcgctgtt tttgttgttc
     7441 aaacgcaagg cgaataaggt attggcggct tgagcctgtt gtgcataaat cgccatatca
     7501 tcgcgttctt gcactttggt aaaaaagccc tctgggcgtt gttgtaaaga aagcgtataa
     7561 attccctttt ggtgtttgcc agaaagacgg aatgcgtgtt tatctgctgt gccatttact
     7621 ttgataattt gatgcccatc gaggcttttt aaatcgtcta ttggattttc gaagatgatg
     7681 tcggaagtgc cagtaacatt tttctcaaaa attaatgcag tatttttcgc ttctttagga
     7741 tcgtaagcaa aacgaaaacg agctccgcca gcataatctt cttttacgag taaactttca
     7801 cttttagtat taaaacggat gtctgcattc gttgttttta atttcccaac attagaatcc
     7861 caacggggct cccagagaga attttctaag cggaattcat ccaaactaat cgtttgcccg
     7921 ataacgtgcg agttgtctgt aacctcaata tagtggaatg gatctaaacc agaatataga
     7981 tgtgctgcaa aagaaacata attttcaata tgatgaatta cttgattagc ccattctgta
     8041 taattcccga cagataaaat ttcgctgttg atatgactat tttttatttt tggacctaag
     8101 gagaatatat gactttttac tataagagga tgggatccaa atttttcagc ttggcaagta
     8161 ctataatcac gtatcttagt gttagaatta aaacattcct taaaatattt ccgtatttgt
     8221 tcttctgtgt ccccatttct ttttgcaacc cctaaacctc gggcgaagcc aactaggtaa
     8281 ccttcggtat attcttgatc ataaaaagaa atcttttttg agttattgat gttttcgaat
     8341 tggtatgttc tagggtatag tgcgggaaag ggtggaactt ttggattatc ctcggttata
     8401 agataagttt cttttttcca atattcactc gttttatcgc ggagtttttt taagcgggta
     8461 atttcatcat tagtgagctt ggttttgtcg taaacgtaat caacagccaa aagcggagag
     8521 gtataaagaa tagaaaaaaa tagacttaca ataaatgatt ttttaaactt ctgcttgctt
     8581 gcttgcttgc ttcgagtttc ataataaatt ttcctttgtc aagtaaaaat aaatggggcg
     8641 tggattttag cataaaactg aacaaaaaat gtcatttatc tcacattttt ctctatttat
     8701 ttcttgttta ttaaaagtaa acgtttgctt tttgctattt tgtcaagcca gtttgaaaat
     8761 gtgtataatt gccctcgtta tttacaaaaa tttcaggaaa aatgaccgca ctttaccctt
     8821 ggctaatgcc aatttatcat caaattgctc aaacctttga cgaaagcttg gggcatcatg
     8881 ccgtgctgat taaagcggat gctggtttag gtgtagaacg tttacacatc aggcggcagc
     8941 cttgcccata ccgtctgaag cactgtttcc acaatcagcg cgtatgctta atcaaccgct
     9001 gtttctcgcg tttccaatcc gcctctttca tactctggcg tttgtcgtgc tgtttcttac
     9061 cttttgccaa accgatttcc atcttgattt ttccgcgtga aaaatgcaaa tccagcggca
     9121 cgatggtgta gccggcacgt tcggttttgc cgattaattt gttgatttcc gactggttca
     9181 acaagagctt gcgcggacgt acggcatctg gtttaatgtg tgtcgaggct gtgggcaaag
     9241 ccgtaatatg gcagccgacc agataaaacg cgtctttttt ccaatagata taactctctt
     9301 taagctgtac gcgcgcggcg cggattgctt tgacttccca gccttccaag accaaaccgg
     9361 cttcaatccg gtcttcaatg aaaaaatcgt gaaatgcttt tttattgttc gcaatagcca
     9421 taaacatcct atcaatatcc gccgtcagac ggcataaacc cgaaaacaga acccatcata
     9481 ccgcctcttc aaccgcctgc acaatcttct cgggatacag cctgttgagg cagtcggtat
     9541 gccccagcgg acattcccgc ttaaaacacg gcgaacattc caagtgcagg ctgacgattt
     9601 tcgccctatc gctcaaaggc ggcgtatgcg tcgggctgga agaaccgtaa accgccacca
     9661 ccttcctgcc caaagctgcc gccaaatgca tcaatccgct gtcgttacac acgaccgtgt
     9721 ccgccaacga cagcaaatcc attgcctgcg acaaatcggt tttgccgcac aaattgacac
     9781 acataccgtc tgaaaggcgg ttgatttcct cggcaatttc atcatctttt tgcgaaccga
     9841 acagccaaac ctgccaaccc gccgccagat aatgtttgcc caactcggca aaatgccttg
     9901 tcggccaacg ctttgccggc ccgaattccg cacccggaca aaaagccaga acaggctttc
     9961 caatatccaa gccaaaggtt tcgacagaaa tttcccgccg ccgttcatca atggaaaact
    10021 cggggaatcc cgaatgcccg tcaaaatctt cctgactcgg atgcgcgaga gccgtatatc
    10081 gatccaccat caaaggcaga cgttccttat ccagcctgcg tatatcgttc aacagaaaat
    10141 aacggctttc accgacataa cccgtccttt taccgatacc tgtcgccagc gcgatgattg
    10201 ccgatttcaa agaaccgggc aacacgataa cctgatcgta tccgcgccgc cccaaatccc
    10261 taccgacccg ccaacggcgt ttcaactcca acgcaccatg tccgaacgaa ttctcaagaa
    10321 tttcattcac ttccggcata cgctcgaaca ccgccatcga ccacttcggt gcgaacacat
    10381 caatcgtgca accggggtga agttccttca aacggcggaa caagggctgg gtcatcacgc
    10441 agtcgcctat ccaactgggg gaaataatca ggattttgat ggacataaca agaaaccgaa
    10501 atcagacagg cagaatttta ccgcgaaacc gttggaaaac ctatcttgcc gcattccgaa
    10561 cgccggacgt gcaaatatga aaaagcccga acattcaagt tcgggcttca aaattctggc
    10621 tccccgacct gggctcgaac cagggacctg cggattaaca gtccgtcgct ctaccgactg
    10681 agctatcggg gaatggggcg tattatagcg tccggaaaaa atgtgtcaat ccttaatttt
    10741 ggaaaaatgg gcgacaaaac gacaagcata tgaatcagaa agacattaag accgatgcct
    10801 taaaaggatt gccgttgtat gaatttccac agccgtcatc acaccatatt taagcccgat
    10861 gagccgttct gccctccccc cgcttaaaac aatgccgtct gaacttcgcc gtgttccaaa
    10921 gccagtaaaa actgtttgcg gttcaacccg cccgcgtagc cggtcagttt gccgtcgctg
    10981 ccgatgacgc ggtggcaggg aatcaggata gatactttgt tctgcccgtt ggcggcggca
    11041 acggcgcgga cggctttggg gttgcccaaa cgctgcgcct gctccttgta gctgcgcgtt
    11101 tcgccgtaag gaatcgccaa gagcgcgtcc catgcctgct tttgaaactc ggtgccaatc
    11161 tgctccaaag gcgtggcaaa ggttttcaga cgacccttga agtataagtc caattcctgc
    11221 cgcaaaagtt gcgtccgctc atcctcccga aacacaaacc gtccgcgcaa ggctttttgg
    11281 acggcggcaa tttcctgttc caaatgcttc tgtccgacaa attccagcaa acacaaaccc
    11341 ctgctaccga acaccgccag catctcgccc aaaggcgtgg caatggcggc acacaccagc
    11401 tcgttcaaac tgtcgggata acgcgcttcc aacagacgga tggcgcggcg gatgcggaca
    11461 tattcttcag gcgcgcagcc gatattgtcc caaaaatccc gctcgaactg tttggcttcg
    11521 cattccgtca gattgggatg cggcataacg ccgcactcaa aaacccgaga ttcgagccaa
    11581 tggcggattt catcccattt tgacggcaga ttgtttaagg aaggcagggt aatcatttgt
    11641 ttgctccgta tccctatcat agatttgacg gcaaaatccc caatttttgc cattcccgca
    11701 cgccggagca ggaacgggct atgacgtaaa tcttgagggt taggttgcgg caatacctaa
    11761 atattcgata tttctaaagc atcagagaaa ggaatgtttc aacacacagg acgacacata
    11821 aagcgccgcc ccatgaaaaa tttcagacga cctgcaaagg gtcgtctgaa accacgattt
    11881 ttgcatttgc gcattctggc acatcatcca accgtttcgg cacattcctg ccgccgttga
    11941 cagcctataa tgaatccact tattcatcaa gcaaaggaat catctatgca aaccctcatc
    12001 ctctccgccg tactgctggc tttttcaacc gctgcctttg ccgggggcgc attcacgctg
    12061 caattcgaca acccgtccga agacggcggc ttcacgcaaa accagctttt gagcgcgcct
    12121 tacggctttt gctgttcagg cgacaatgct tcgcccgcgc tgtcgtggaa aaatccgccc
    12181 gccgggacaa aagtttcgtc ctgaccgttt acgataaaga cgcgccgacc ggactgggct
    12241 ggatgcaccg ggtggtcgcc gacattcccg ccgatgtcca ccgccgcaac gcgacctcgc
    12301 tgcaattaag ccgctgcgcc aacatcgccg accggactgg gctggatgca ctgggtggtc
    12361 gccgacattc ccgccgatgt ccgccgccgc aacgcggcct cgctgcaatt aagccgctgc
    12421 gccaacatcg ccgacgacca gtccgcagcc atatcggcgg taatcagttt gcggatttgc
    12481 cgcatcaggt tgacgccttc gtacacggca aaaccgatgc cgtcatgctg caaccacgcc
    12541 aacacgccgc aaagcgcggc ctccgcagca ttgtgcggca cttcttcatc cgccagtacc
    12601 gcagcctcat aatcaaacgc cgcgcccata cgcccggaat acggcagctt taccgcatcg
    12661 cacactgcct gcgccgtccc gtattgtgcg gcgaaccttt ctacggtttc ctgttcgaaa
    12721 gcaatccatt gcgcctgata gaggccgtct gaatcgggaa tattgatgac gtcaaacgtc
    12781 tgtccgcctg ccaaggcgac cgccttaccc gccgcagctt cttacttccg cgccgcacga
    12841 taagcacagc cggttcatat accgccacgc tgcggtacaa ggcggtatga tgttgcacga
    12901 tgccgcctaa agcacccaat cgttcgcgcg tatgaaagta tagtggatta aatttaaatc
    12961 aggacaaggc gacgaagccg cagacagtac aaatcgtacg gcaaggcaag gcaacgccgt
    13021 actggtttaa atttaatcca ctatatctca aacccacgtt aggtctaagc aaatggtcgg
    13081 acatccttat ccgacagccc atcttctttt cagacggcat tgcaaattta agtttgacgt
    13141 gcgttcaaaa taaggcagtt aatgcgaagc gaaattccgt cggcgtacct gcaacttggc
    13201 ccctccccta taggggaggg tcggagggag ggtaaaacgg ggcagataca gacaatattt
    13261 ccgttgccgc cccgatgccc tctccctaac cctctcccac gggagaggga atggattgcc
    13321 gttgaaataa atcgctctac ataaaaaatc aatgtgttat ctcaaaccca cattaggtct
    13381 aatcaaatgg tcggatatcc atattcggca agcaagctgc tttcagacgg catttccagc
    13441 caacaagcgc gccaatatcc cctcatacac cgcagacagc ttcggaatgt cgtttagccg
    13501 cacgttttcg ttgatttggt ggatggtcgc attggacggg cctaattcga taagttcttg
    13561 cgcaatggct ttgatgaagc gtccgtccga agtgccgccg gtggtggaca attcggcctc
    13621 aatgccgcag gtttcggcaa tggctgcgcg tgccacgtcg gtcagtttgc ccgcttgggt
    13681 cagaaagggc tgccccgaac acgaccactg caaatcgtat tgcacgccgt gtttgtccaa
    13741 aatggcgtgg acgcgttgtt tcagccctgc ttcggtggac tcggtggaga agcggaaatt
    13801 gaatttgacg ttcagctcgc ccggaatgac gttggtcgcg cctgtgccgc cgttgatatt
    13861 ggaaatttga aagctggttg gcgggaaata ttcgttgcct tcatcccaga cttcctgcgt
    13921 cagctctaac aaggccgggg caaaagtatg cacgggattg attgccaaat gcggataggc
    13981 aatatggcct tgcttgcctt tgacggtcag gttgcccgac agcgagccgc gccgaccgtt
    14041 tttaatcata tcgcccaatt tgtccacggc ggtcggttcg ccgacgatgc agtagtcgat
    14101 aagctcgtcg cgcgctttca atacatcgac gactttggtc gtgccgtcca acgcgtcgcc
    14161 ctcttcgtcg gaagtaatca gaagcgcaat gctgccttgg tggttgggat gtttggcaac
    14221 gaagcgttcg caggcggtaa cgaaacaggc aatgctggtt ttcatgtctg ccgcgccgcg
    14281 cccgtataat cttccgtcgc gctcggccgg ttcgaacggg ggcgaatccc atttttcgac
    14341 aggacctgtc ggtacaacgt cggtatgccc tgcaaaacag acgacgggag ctttcgtgcc
    14401 gcgtcgcaac cagatgtttt tggtgtcgcc gaaatggagt tcttcagccg caaaaccgat
    14461 tttgtgcagg cgttcggcaa ggagtttttg gcaatccctg tcgtcagggg taacggatgg
    14521 tcgggaaatc agctctttgg caagctctag ggattgagtt tcggtcatat ttgttcactt
    14581 ttgaaattag accgtctgaa acgttctgaa tgtgattttc agacggcatt taggttaggt
    14641 tggcatacgg ggtgggtatt ttacccatca gtcttctgaa tcatttgccg tggcaggctt
    14701 cgtaaagcgg cagcaaatct tccaccgttt ccgctatcca tttcgcgaca tcctgcctgc
    14761 ccaaatcgtc gcgttcgatg tgtttgccga tgcagaaaaa gtcttcgtcg ttttgcaact
    14821 ttcggtcgga ctcgttttgt tgcgcgacgg tgcggtagtc gtcgtattcg ctttccgcac
    14881 cgtgccacat atcgaaagaa gcgtattttt cggtatcaaa attatccaac cagcggttgt
    14941 aatcaggcag cgcgatgggg gaaacatcgg ctttatagca gtgccaatcc aagctgacgc
    15001 tcagacggcg gcggttgagc agtatcgaca aaatcgctgc ggaattttta tattgttcgt
    15061 atttgaagta ggcaaagaaa tgggcgcgaa cctgccagcc gttacaccag cgttcgatgt
    15121 gcggcggcgc aaacggcgca cccaattcgg cggcaacctg ctgaatcagc tgctgccata
    15181 tctgccagtt ttctttatag tcagccttga tttgcggaat gctttcaggc tggtattttt
    15241 taagctggga aaattggaaa aacgggatat tgaacaaatc gcaacttttc ggggtcagca
    15301 taatatatcc ttgagacgat tgtttcagac ggcattattt gcgccggcgc gccgccataa
    15361 tttcgccgat ttcggtcagt ttttcttttg ggataaaggt gttgcccata tcaaacagcg
    15421 gctcttcaat cgccaaatga acatcatatc ccgccacaaa acgtttgaac gcttcctcat
    15481 cggggacata agcgttgtct gcttcgagtt tggcaaattc ggcggaaaca gccgcccagt
    15541 tgtcgtgcag cccgatatgt tggcgcaaaa gctcgtccac gctttcttgg gcttgcggcg
    15601 catattgcag cagcagcggg aagaagtttt cttcttcgtc ttcatggtgc agcggcgcgg
    15661 caacgttgaa atactgggcg atttggcgga tggtttgcaa aacaatctga ttgcagccgt
    15721 tttcggcgat atagtccgac agcatggcga cttgtccgca aaaacggcgc actttgccgt
    15781 ggcaggcata cagcatttca atcggttcgg caaaggtaac gcttttggtt tcaaacggat
    15841 tcatgttttc gttctcaacg ggcacttttc aagcagtcat tttataataa aacagcctgc
    15901 acaaagcagg ctgtccgtct tttgagactt taagcggatt aatcgaccaa agtcactttg
    15961 ccgttcatca aagcaccgtg acctgggaag gtacaagcga atttatattc gccgtcggcc
    16021 aatttagcag gatccagagt cagggaagct tcttcgccgc cgccgatcag tttggtatgg
    16081 gcaacaacgc gtgcatcatc aggtttgaca tagtcggtat cggcagcacc tacgccgtct
    16141 ttaaatacgc cgtccatgtc ttcagctttg gcaatcacga gattgtgacc catgctggct
    16201 ttgggttgcg taccggtatg tttcagagtg atggtgaact ctttacatgc tttgctgact
    16261 tggatgtctt tggtgttgaa ctgcatattg tcgttggatt cgacagttgc cgcacagttg
    16321 ccggcagcag gggcttcggc agcatctgca ggagcagctt cggcggcagg cgcttcggaa
    16381 gcgggtgctt cagcagcagg agttgcctcg gcagcaggcg cggcaggttc ttgagagcag
    16441 gcagccaaac cgataacggc ggcagaaatc agagccagat acgctttcat aacaaatctc
    16501 caatcgataa aataatattc ggttttacag aaatcaaagt gcaaccgcca ttaacaaaac
    16561 cttgaaaaag attccgccgc gttgcacaaa cagatgtttc ggagcggcat tttgctacaa
    16621 atttcatttg aaatcaaagc ctgtttgcaa gtttacaatc gtttacccaa aaaagggcaa
    16681 ttttaccccg aacctatttc tttagtatta gacctattat cctttacttc ttaatattaa
    16741 cggatgttta cacaaattcc cgtatacatt ttatgcgcca tgccttctaa ccaagtttgc
    16801 caatgcctcc gccaattcgg gatgccgttt ttccaacttt gccgccgccg aaccgaaact
    16861 ctccagcgca gccttactca aatgcagggt attggttttc ggcggttttt ccggtttcgg
    16921 gaccagcctg accgaaacag agcgtatcga agcatcaagc cctgccaact gcggcaatac
    16981 cgacggtgca atcattttca agcgcgatgc cgccatattg tttgccgcca aaaggacaag
    17041 cctgccgtct tcgatacatg ccgtctgaaa atgcgggtgc aggttggcag gcagcagttt
    17101 tttcacggcg gcatccaacc gccgccactg tcccgcctgt ttcaaaagtc cggaaagcag
    17161 cgcgtcccgc ctgcccaact gttccaaatt cataaaacat acacccaaaa agattgaaat
    17221 accgcaaacg cgcctttatt tcagacggca ttagcacttt gcacaaacgc ttgtgttaaa
    17281 atcgcgtttt cgcccactat tatatcaggc gcaggaatta ttcatgctga caaacattgc
    17341 caagaaaatc ttcggcagcc gcaacgaccg cttgctgaaa caataccgta aatccgttgc
    17401 cagaatcaac gcgctcgaag aacagatgca agccctaagc gatgctgatc tgcaagccaa
    17461 aactgccgaa ttcaaacaac gcctcgccga cggtcagact ttggacggca ttttgcccga
    17521 agccttcgcc gtctgccgcg aagcgtcccg ccgcaccctc ggtatgcgcc acttcgacgt
    17581 gcagcttatc ggcggtatgg tgctgcacga cggcaaaatc gccgaaatgc gtaccggcga
    17641 aggcaaaacc ttggtcgcca ccctcgccgt ctatctcaac gcgctggccg gcaaaggcgt
    17701 acacgtcgtt accgtcaacg actacctcgc ctcacgcgat gcgggcatta tggagccgct
    17761 ctacaatttc ctcggcctta ccgtgggcgt gattatttca gatatgcagc cgttcgaccg
    17821 tcaaaacgcc tatgccgccg atatcaccta cggcaccaat aatgaattcg gcttcgacta
    17881 cctgcgcgac aatatggtta ccgaccaata cgacaaagtg cagcgcgaat tgaattttgc
    17941 cgttgtcgat gaagtggatt ccatcttgat tgacgaagcg cgcactccgc tgattatctc
    18001 cggtcaggcg gatgacaaca tccagttgta ccaaatcatg aacaccgttc cgccccacct
    18061 cgtccgtcaa gagacagaag aaggcgaagg cgactattgg gtcgacgaaa aggcacatca
    18121 ggtcatcctg agcgaagcag gtcacgaaca cgccgagcaa atcctgaccc aaatgggatt
    18181 gctggcagaa aacgactccc tctattccgc cgccaatatc gccctgatgc accaccttat
    18241 ggcggcattg cgcgcgcatt ccctcttcca caaagaccaa cattacgtca tccaagacgg
    18301 cgaaatcgtc atcgtggacg aattcaccgg ccggctgatg tccggccgcc gctggtcgga
    18361 gggtctgcat caagccgtcg aagccaaaga aggcgtggaa atcaaacgcg aaaaccaaac
    18421 gcttgcatct attaccttcc aaaactattt ccgcctgtac accaagctct ccggcatgac
    18481 cggcacagcc gataccgaag ccttcgagtt ccaaagcatc tacaacctcg aaaccgtcat
    18541 cattccgacc aaccgccccg tacagcgcaa agacttcaac gaccagattt tccgttccgc
    18601 cgaagaaaaa ttcgaagccg tcgttaaaga cattgaggaa tgccacaaac gcgggcagcc
    18661 cgtcctcgtc ggcaccacca gcattgaaaa ctccgaactg gtatccaagc tgctgaccca
    18721 agccggactg ccgcacaacg tcctcaacgc caaagaacac gaacgcgaag ccctgattgt
    18781 cgcccaagcc ggcaaagtcg gcgcgattac cgttgccacc aatatggcgg gacgcggtac
    18841 ggacatcgtt ttaggcggca acctgaagca ccaaaccgat gccatccgcg ccgacgaaac
    18901 cttgagcgac gaagagaaac aggcacaaat cgccgcactc gaagacggct ggcaggcgga
    18961 acacgacaaa gtgatggaag caggcggttt gcacatcatc ggtacggaac gccacgaaag
    19021 ccgccgcatc gacaaccaat tgcgcggacg ttccggccgt cagggcgacc ccggatccag
    19081 ccgcttctat ctctcctttg aagacccatt gctgcgctta ttcgcactcg accgcgccgc
    19141 cgccatcctc aaccgcctcg cccccgaacg cggcgtcgcc atcgaacaca acctgctgac
    19201 gcgccaaatc gaaggggcgc aacgcaaagt cgaaggcaga aacttcgata tgcgcaaaca
    19261 ggttttggaa tacgacgacg ttgccaacga acagcgcaaa gtcatttaca gccagcgcaa
    19321 cgaaattctg accagcaaag acatcagcga cctgatgcag gaaatccgtt ctgatgtcgt
    19381 cagcgacctc gtggatacct atatgccgcc cgacagcatg gaagaacaat gggacatccc
    19441 gactttggag aaccgtctgg ctgccgaatt cagactgcac gaagacatcc aatcctggct
    19501 gaaggcggac aatgcgattg acggtcaaga catcaaagaa cgcctgatcg aacgcatcga
    19561 aaacgaatat gccgccaaaa ccgaactggt cggcaagcag gcaatggccg atttcgagcg
    19621 caacgtgatg ttgcaggtca tcgacaacca atggcgcgaa cacctcgccg ctatggacta
    19681 cctgcgacaa ggcatacacc tgcgcagcta tgcccaaaaa aatccgaagc aggaatacaa
    19741 acgtgaagcc tttaccatgt tccaagacct gtggaacggc atcaaattcc atattgcctc
    19801 cctgcttacc tcggttcaaa tcgaacaaaa ccctgtcgcg gtggttgaag agcaacccat
    19861 cggcaacatc cagtccatcc attccgaatc gcccgatatg gaagaacttt tgggtcagtc
    19921 gcaaaccgat ctggttaccg aagcctttaa tcccgatggg acagatttca gccccgaagc
    19981 cttggaagcg cgggggcaaa tcgtccaccg caacgacccc tgcccctgcg gcagcggttt
    20041 gaaatacaaa caatgccacg gcaaactggc ttaagcgttt gaacgcaaat gccgtctgaa
    20101 catcccgctc ccgtttcaga cggcattttg cctgaaccgc cacatccgac tgccattccg
    20161 aaaaatcccg atttcgtacc gtccgtacca aaaacagaca tcccgtccgc cccacatcat
    20221 gattccatcc gacttcattg acgagctttt agccaaaacc gatattgtcg atattatcga
    20281 cgagcaggtt ccgctgaaaa aaggcggggc gaactatatg gcgtgttgcc cgttccacaa
    20341 ggaaaaaacg ccgtcgtttt cggtcagtcc aaccaagcag ttttaccatt gtttcagttg
    20401 cggggcacac ggctcagcga ttggttttgt gatggaacat cagggactgt cgtttccgga
    20461 ggcggttcag ttccttgccg accgcgtggg tatggtcgtg ccgaaagtgc acgggcaaaa
    20521 cgataatccc gaagtccgtg ccgaacgtaa gaaaaaacag cagacactgg aggaaacgac
    20581 ggctgcggca gctgattttt acgcgcaaca gctaaaattc aatccagcgg caaaagctta
    20641 tttggacaag cgcggcttga gtgcagaagt tatcgcgcat tatggtttgg gctatgcgcc
    20701 cgacggctgg cagcctttga cgcaagtgtt ccaaccgtat cctaataccg cgttagtgga
    20761 tacggggatg gtgattgaca atgagggacg gcattacgac cgcttccgcc atcggattat
    20821 gttccccatc cgcaatccgc gcgggcaggt tatcggtttc ggcggcaggg tgctggacga
    20881 ctcgaagccg aaatatttaa attctcccga tacgcctttg ttcgataagg ggaaaaacct
    20941 ttacggactg tatgaagggc gtgccgctgt caaggaagcg gggcggattt tggtggtcga
    21001 aggctatatg gacgtggtcg cgctggcaca gttcggcgtg ggctacggcg tggcggcttt
    21061 gggtacggcg acgacggcgg aacacgtcaa aatcctgatg cgtcaggcag acagtattta
    21121 tttctgtttc gacggcgaca gcgcggggcg aaaagcggct tggcgcgcgc tggaaaacgc
    21181 gctgccgcag ttgaaggacg acaaatcgct gcattttttg ttcctgccgg aagaacacga
    21241 ccccgacagc tacatccgcg cctacggcaa agcgcaattt gaagacgcgc ttctgaatca
    21301 aagcaagcct ttgtcggagt atttctggga acacctttca gacggcattc atctcaatac
    21361 gcaggaaggc aaggcggaat tggtaaaaac cagttcgccg cttttggcgc agattaccgc
    21421 gccggcattg gcttatttgt taaaacaacg gcttagcgaa ctggtcggca tcgaccccga
    21481 caacctcgcg caactgctgg gacaggaagc gccgaagcgg cacgtcaaac aaaaaaacta
    21541 caaactgccc ccgatttccg tcaaacagcc cgttatgccg acattggtgc aacggcaaat
    21601 ccgcagcctc ttgataaatc cggattgggc tgcatatata gacctgcccg attatctggc
    21661 gttggacggc gatttcgcct gccttgccaa cctcgccgaa accattaaaa accatccttc
    21721 cgtgccggca accgcacagg ttttggaaca tatgcgcggc tcgccttacg aagaaacgat
    21781 aacccgaatc ttccattcaa cgcaccaatc ggaagaaatg aacagcagca gtgaagaaga
    21841 ttgcgagaat ttccaaatcg gcatgaaaaa actgctcaat gagttaaaat acagccaaat
    21901 cgaaacatta aaacaaaaaa gcctgcaatc cggcttaaat gaaagcgaga aaaaactttt
    21961 gctgtcgctg ctgaccgcaa aacaaaattg accggcggat tccgccatcc gtaaaccgtt
    22021 atgccgtctg aaaagcactt accccggctg caacaacgac acctgcagaa cacccatccc
    22081 aaaaagcctt cagacggcat cagagtaccc gctctgccac gccttcaggt gcgttcaaac
    22141 gcaaaccgtc ggcaccttac caacagaaag cagacaatgt ccagaaacca aaatcacgaa
    22201 gaataccaag acgatgcccg ccccctgacg attgaggaac aacgcgctcg cctgcgccaa
    22261 ctcatcatta tgggcaaaga acgcggctac atcacctact ccgaaatcaa cgacgcactg
    22321 cccgacgata tgtccgatgc cgatcaaatc gacaacatcg tcagtatgat ttccggtttg
    22381 ggtatccaag ttaccgaaca cgcccccgat gcggaagaca tattgttaag cgacaatgcc
    22441 gccgttaccg acgatgatgc cgtcgaagaa gccgaggccg ccctttccag cgcagattcc
    22501 gagttcggca ggacgaccga ccccgtccgt atgtatatgc gcgaaatggg acaggtcgac
    22561 ctgctgaccc gcgaagacga aatcatcatc gccaaaaaaa tcgaaaacgc cctgaaaaat
    22621 atggttcagg ccatctccgc ctgcccgggt tccattgcgg aaatcttaga actcatcgaa
    22681 aaaatccgca aagacgaaat ccgcgtcgac gaagtcgtag aagccattat cgacccgaat
    22741 gaagtattgc tcaacgaatt gggcttgggg cacttggaaa ccacagcacc cgagaaacct
    22801 tccaacgaca attcggacga aaacgaagac gacgaagaat cggaagaaga tgcggatgaa
    22861 atctcggcag ccaatctcgc cgaattgaaa caaaaagtca tcggccactt tgcccaaatc
    22921 gaaaaagact acaaaaaaat gattggccgt ttggaaaaac accacagccg gcacaaagac
    22981 tatctcgcct accgcgacgc gattgccaac aaacttttgg aagtccgctt cgccacccgg
    23041 caaatcgaca gcctcagcag cagcctgcgc ggaaaagtag aaaacatccg caaactcgaa
    23101 cgcgaaatcc gcgacatctg cctcgaccgc gtccatatgg agcgcgacta cttcatccaa
    23161 aacttcctgc ccgaaatcac caatctggaa tggattgaag aagaaatcgc caaaggcagg
    23221 gtttggagcg acgcgctcga ccgcttccgc cacgccatcc tcgaaaaaca aaccgagttg
    23281 gcggatatgg aaaaagaaac ccgcatttcc atcgaagagt tgaaagaaat caacaaaaat
    23341 atggtgtcaa gcgaaaaaga aaccgcagct gccaaacagg aaatgattca ggcaaacttg
    23401 cgcctcgtca tttccattgc caaaaaatac accaaccgtg gcttgcagtt ccttgatttg
    23461 attcaggaag gcaacatcgg cctgatgaaa gcggtcgaca agttcgaata ccgacgcggc
    23521 tataagttct ccacctacgc aacctggtgg atccgtcagg caatcacccg ctcgattgcc
    23581 gatcaggcgc gtaccatccg cattccggta catatgattg aaaccatcaa caagatgaac
    23641 cgcatctcgc gccaacacct tcaagaaacc ggcgaagaac ccgattccgc caaacttgcc
    23701 gaactgatgc agatgcccga agacaaaatc cgcaaaatca tgaaaatcgc caaagagccg
    23761 atttcgatgg aaacccccat cggcgacgac gacgattcgc acttgggcga cttcatcgaa
    23821 gatgccaaca atgttgcgcc ggccgatgcg gcaatgtaca ccagcctgca cgaagtaacc
    23881 aaagaaatcc tcgaaagcct gacaccgcgt gaggcaaaag tcctgcgtat gcgtttcggc
    23941 atcgatatga acaccgacca cacgctggaa gaagtcggca gacagtttga cgtaacgcgc
    24001 gaacgcatcc gacaaatcga ggcaaaagca ctccgcaaat tacgccatcc gacaagaagc
    24061 gaccgtttga gaagtttctt ggacagcgaa gacagcaagc tgtaaaccaa aaaaccgcag
    24121 gtttcaaata cctgcggttt tttcttacac aataaacaac gcttccacat atcccacact
    24181 cctatcccga gacctttgca aaattcccca aaatccccta aattcccacc aagacattta
    24241 ggggatttct catgagcacc ttcttccggc aaaccgccca agccatgatt gccaaaca
//
LOCUS       DAWIVB010000011        23338 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWIVB010000011 DAWIVB010000000
VERSION     DAWIVB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.011"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            32..6562
                     /locus_tag="WIX60_000309"
     CDS             32..6562
                     /locus_tag="WIX60_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ5304636.1"
                     /translation="MFASSGNVAIDANGRLVNSGTMAAANAKDTDNTAEHKVNIRSQG
                     VENSGTAVSQQGTQIHSQSIQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTD
                     TLNNQGKLSQTGSQKLHIDAQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASFHSS
                     TTTPTTATGTGTATVSISNITAPTFADGTIRTHGALDNSGSIIANGQTDVSAQQGLNN
                     AGQIDIHQLNAKGSAFDNHNGTIISDAVHIQAGSLNNQNGNITTRQQLEIETDQLDNA
                     HGKLLSAEIADLAVSGSLNNQNGEIATNQQLIIHDGQQSTAVIDNTNGTIQSGRDVAI
                     QAKSLSNNGTLAADNKLDIALQDDFYVERNIVAGNELSLSTRGSLKNSHTLQAGKRIR
                     IKANNLDNAAQGNIQSGGTTDIGTQHNLTNRGLIDGQQTKIQAGQMNNIGTGRIYGDN
                     IAIAATRLDNQDENGTGAAIAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNGQ
                     ATGKAQRIHNAGATIEAAGKMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHEL
                     LREGTQHELGWSVYNDESDHLRTPDGAAHENWHKYDYEKVTQKTQVTQTAPAKIISGN
                     DLTIDGKEVFNTDSQIIAGGNLIVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHK
                     GRDSTGHSEQNYTLPEEITRNISLGSFAYESHRKALSHHAPSQGTELPQSNGISLPYT
                     SNSFTPLPSSSLYIINPVNKGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLG
                     DGYYEQRLINEQIAELTGHRRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQ
                     VAQLTSDIVWLVQKEVKLPDGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSG
                     SLKNSGTIAGRNALIINTDTLDNIGGRIHAQKSAVTATQDINNIGGMLSAEQTLLLNA
                     GNNINSQSTTASSQNTQGSSTYLDRMAGIYITGKEKGVLAAQAGKDINIIAGQISNQS
                     EQGQTRLQAGRDINLDTVQTSKHQATHFDADNHVIRGSTNEVGSSIQTKGDVTLLSGN
                     NLNAKAAEVSSANGTLAVSAKNDINISAGINTTHVDDASKHTGRSGGGNKLVITDKAQ
                     SHHETAQSSTFEGKQVVLQAGNDANILGSNVISDNGTQIQAGNHVRIGTTQTQSQSET
                     YHQTQKSGLMSAGIGFTIGSKTNTQENQSQSNEHTGSTVGSLKGDTTIVAGKHYEQIG
                     STVSSPEGNNTIYAQSIDIQAAHNKLNSNTTQTYEQKGLTVAFSSPVTDLAQQAIAVA
                     QSSKQVGQSKNDRVNAMAAANAGWQAYQTGKSAQNLANGTTNAKQVSISITYGEQQNR
                     QTTQVQANQAQASQIQAGGKTTLIATGAAEQSNINIAGSDVAGKAGTILIADNDITLQ
                     SAEQSNTERGQNKSAGWNAGAAVSFGQGGWSLGVTAGGNVGKGYGNGDSITHRHSHIG
                     DKGSQTLIQSGGDTTIKGAQVRGKGVQVNAKNLSIQSVQDRETYQSKQQNASAQVTVG
                     YGFSAGGDYSQSKIRADHVSVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSTQSAEDK
                     GKNRFQTATLTHSDIKNHSQYKGESFGLGASASISGKTLGQGAQNKPQNKHLTSVADK
                     NSASSSVGYGSDSDSQSSITKSGINTRNIQITDEAAQIRLTGKTAAQTKADIDTNVTT
                     DTAERHSGSLKNTFNKEAVQSELDLQRTVSQDFSKNVQQANTEINQHLDKLKADKEAA
                     ETAAAEALANGDMETAKRKAHEAQDAAAKADNWQQGKVILNMLASGLAAPTQSGAGIA
                     AATVSPAVSYAIGQHFKDLAGQNANGKLTASQETAHVLAHAVLGAAVAAVGDNNALAG
                     ALSAGGSEAAAPYISKWLYGKEKGSDLTAEEKETVTAITNVLGTATGAAVGNSATDAA
                     QGSLNAQSAVENNDTVEQVKFALRHPRIAIAIGSVHKDPGSTLEPNISTIASTFQLNL
                     FPNSEFGGEGGVGNAFRHVLWQATITREFGKDIAVKVGNSHESGEKINYSIRRNLSLD
                     KADEMIDQLNNEIGREIALNTNRLNTKELVGLILETYKNNGFYQAERNSNGNYDVVRK
                     RLSEKDYQNTSNILIHLDNTGAGFKIQQRRKQIRAQISARQWRR"
     gene            6566..6982
                     /locus_tag="WIX60_000310"
     CDS             6566..6982
                     /locus_tag="WIX60_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304637.1"
                     /translation="MNKNILYIFSLLITIVIFFIFEKNVIRKISFNYNKKEFLISDIT
                     NFNWDYVKLYIINSDFQKIVFYHKSKIVFEELIELDKEGNVLPQYLFDSDLKNVEYYE
                     CDYKNGKMQLLKKEKSHFFDGYFYYYKPINCRPKLL"
     gene            7051..8064
                     /locus_tag="WIX60_000311"
     CDS             7051..8064
                     /locus_tag="WIX60_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ5304638.1"
                     /translation="MAWSMFATTQADRAVRSATAPKEMWFHKKIIDEKTGKVSFDTRQ
                     IWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAAKQNRNSTNGSGVIAVM
                     NPPTGKYKSDSNNKIKDFLWLGSSLVSELMYVGYDQLNNKVFQGYLPKTNSEKLNQDI
                     YREVQKMGNGWSVDTSNHSRGGITASVSLKDWVNNQKQNGIAPIRKARFYGTATNVQN
                     DYADVLQKNGYTYTGADGKTYNSGSYSIVHDKDFVGNKWIPFLLGTNDTTQGTCKGLC
                     YSHSSYFAEVPKAGTKEFDDYVKIWGEVEYDAQGKPINKSKPILVEPNKTKDNEKYEK
                     EAF"
     gene            8036..8344
                     /locus_tag="WIX60_000312"
     CDS             8036..8344
                     /locus_tag="WIX60_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304639.1"
                     /translation="MKNMKKKLSKYSLFLSSVFCLTACATTLLGTVVVGTALYGTSSN
                     WTTTDKEHQEIMNCLDKALVKLNIYFENDEEKYKNKTVMDIYYQCIKNPNYVVTHNNL
                     "
     gene            8356..8670
                     /locus_tag="WIX60_000313"
     CDS             8356..8670
                     /locus_tag="WIX60_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304640.1"
                     /translation="MKKTLSNLVLISFCSTMLTACPFWGSCADWNCSKLNPRWEKAVD
                     TCETENLTFVLHELGNQLGYKSITEVSFSEIGQHISYQCIHHGGGYNIRKDKQYGHLF
                     VQ"
     gene            8836..9294
                     /locus_tag="WIX60_000314"
     CDS             8836..9294
                     /locus_tag="WIX60_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ5304641.1"
                     /translation="MPITPPLNIISPKLYPNEQWNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILPVLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNSDWNCG
                     DNIWLIQWFAPLGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            9349..11136
                     /locus_tag="WIX60_000315"
     CDS             9349..11136
                     /locus_tag="WIX60_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEZ5304642.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHARF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPSYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     LGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWMRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLSGQTLVGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
     gene            complement(11461..12750)
                     /locus_tag="WIX60_000316"
     CDS             complement(11461..12750)
                     /locus_tag="WIX60_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEZ5304643.1"
                     /translation="MMKSILTVSGNRMRKPRITYLDVWANDERIGTLEKGAMYRFAYD
                     NPNSSLLGLHYQDRSKVYISNNMPHIFAQYFPEGFLDAHITSKYAFHDAPFEDNEMLR
                     LAILCRETLGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAIRRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IAVAQTSLSEDSSVLLVRRFDVSEQGYFLGMEDFTSLRQYSVEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPVYDVLDTSIYR
                     VGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
     gene            complement(12877..13383)
                     /locus_tag="WIX60_000317"
     CDS             complement(12877..13383)
                     /locus_tag="WIX60_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304644.1"
                     /translation="METTFALLNFKESLCYIYPPTEPVRYVSSIVALNVHSPNGTGDW
                     HSAKALSDRAYPEKFYIYGENQERNTNHLFGDNGIIDGTDRLNKMGYFPENIPVWLAD
                     HPRACVDYLYTAVLQTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWENLQL
                     WKRKNPIM"
     gene            complement(13744..15108)
                     /locus_tag="WIX60_000318"
     CDS             complement(13744..15108)
                     /locus_tag="WIX60_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ5304645.1"
                     /translation="MQHLTERMTQIAKLLNSSANNPDIDIPDFLTEIKDYSEFSVTDE
                     NGTYLHWDKFRRIHTEDTRMKWRAVKESRKKIQKPIDFPFEHQFWFCIPDSLQARLHL
                     IDKSCGSSIGTSSLGGFGRSEQNRFLLKSLIMEEAITSAQLEGAATTRKVAKDMLKSQ
                     RKPKTKDEIMIVNNYHLMKKAVELKNTPLSVEMILDLHRIATSNAIENKAEPGQFRQD
                     DEIFIADINGNSLYQPPPHGQVHTLMEEVCAFANNTYDGVENPFIHPVVQAIILHFLI
                     GYIHPFGDGNGRTARALFYWFMLKNGYWLFEYISISRLLKNAPAQYAKSYLYAETDDL
                     DLTYFIYYQCDIIKRAVADLEHYISDKQKHQQEFKAAIAQYTEKIGKLNQRQIGILQK
                     AVEESGKIFTAQEIANQYGISLNTARSDLSKLGEYRFLVPFKSGNALEYVAPQDLLER
                     LEKK"
     gene            15529..15891
                     /locus_tag="WIX60_000319"
     CDS             15529..15891
                     /locus_tag="WIX60_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ5304646.1"
                     /translation="MKAARRQLYYTFANRIAEQSRIICIKKLNLLNLQCIQIKVNKLH
                     QKIVASSLLLHMLKEGAAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNMVCE
                     SFGRHYDTDENASSNIRK"
     gene            complement(17426..17863)
                     /locus_tag="WIX60_000320"
     CDS             complement(17426..17863)
                     /locus_tag="WIX60_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HEZ5304647.1"
                     /translation="MSRYLITFDMDTNCLKDNYHGNNYTNAYSDIKTILARHGFENIQ
                     GSVYLGREGISEAHGTIAIQELTARFDWFYSCISNIKFYRLESDLNAQFIADGVYQAK
                     QAFLQRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
     gene            18798..19214
                     /locus_tag="WIX60_000321"
                     /pseudo
     CDS             18798..19214
                     /locus_tag="WIX60_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360042.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            19390..19626
                     /locus_tag="WIX60_000322"
                     /pseudo
     CDS             19390..19626
                     /locus_tag="WIX60_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086896509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19679..19867)
                     /locus_tag="WIX60_000323"
     CDS             complement(19679..19867)
                     /locus_tag="WIX60_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ5304648.1"
                     /translation="MTVKYTIFFSTSHHSNQTKATHRHTHVGSTAGQTTIRSGGDTTL
                     KGAQLIGIGIQADPQLQP"
     gene            complement(20012..20962)
                     /locus_tag="WIX60_000324"
     CDS             complement(20012..20962)
                     /locus_tag="WIX60_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ5304649.1"
                     /translation="MYLGIDVSKLTIDCCLIVDGQNYQKKFQNNKGGFEQLINWLQSH
                     KVNDKLHCVCEATGTYYEALAEYLYSRYTITVENPRKIKGYAIAELQRSKTDTQDAKL
                     IAQYCQDRKHKLKAWKPPTKEQKQLQEIARYLDYLKQQRATEKAKQHEAPDYIKSHIQ
                     TTISNLTAQIQIVKKQLLQFYKDNPSYNNLRKRLKTITGIGEQATAVLLSTYKRHEFK
                     NARQFTAYLGLDPRKFQSGTSVNGKSRISKIGSSEIRKSLYMPALVAYRCNAFPEFVG
                     RLKNKGKHIKLILIAIMRKLAVIAFTILQNGQDFQVERYK"
     gene            complement(21588..22673)
                     /locus_tag="WIX60_000325"
     CDS             complement(21588..22673)
                     /locus_tag="WIX60_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HEZ5304650.1"
                     /translation="MIYLFTGNMGTGKTSRVVSMILNNEDGLFKMKLEDGTEVDRPLY
                     FCHIDGLDKRQFKAHELTEEQIMSAPLRDVIPEGAVLIVDEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTQHPSQLDIFVRNLVSKHVHLERKAIGMKQYYWYKCVTSLD
                     NPAGVSGVEVASWKPPKEAFKYYKSASQHQKFKKKVPWAVWALIAIVGFVGWKSYGIF
                     KVYSKATDSRIEQEAQKESVVQTMTEQPASSEEMPLKNSDNLKPEDFVPTLPEKPESK
                     PIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYK
                     DEQQRTEQVEQSAKADKPQVLVMGGKP"
     gene            complement(22683..22973)
                     /locus_tag="WIX60_000326"
     CDS             complement(22683..22973)
                     /locus_tag="WIX60_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ5304651.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLIALEKFK
                     DYTSNAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(22974..23306)
                     /locus_tag="WIX60_000327"
                     /pseudo
     CDS             complement(22974..23306)
                     /locus_tag="WIX60_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003780465.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
BASE COUNT         7056 a         4994 c         4961 g         6327 t
ORIGIN      
        1 cgagcaagca ggcattcgta atcaagggca gttgtttgct tcttccggta atgtggcgat
       61 tgatgcaaat ggccgtttag tcaatagtgg cacgatggct gccgccaatg cgaaagatac
      121 ggataataca gcggaacaca aagtcaatat ccgcagtcag ggcgttgaaa acagcggtac
      181 ggcggtatcg caacaaggca ctcaaattca cagtcagtcg attcaaaaca ctggcacatt
      241 attgtcctca ggcgaaatat tgattcacaa ttcgggcagc ctgaaaaatg aaacatcagg
      301 caccattgaa gccgctcgtt tggctattga taccgacaca cttaataatc aaggcaaact
      361 ctctcaaaca ggttcacaaa aactccatat tgatgcacaa ggcaaaatgg ataaccgtgg
      421 ccgcatgggt ttacaagata ccgcaccaac cgcgtcaaat ggttcaagca atcaaaccgg
      481 caatagttac aatgcatctt tccattcatc cactaccaca ccaacaacgg caacaggtac
      541 gggtactgca accgtttcta tatcaaacat aactgcgcct acctttgctg atgggacaat
      601 tcgcactcat ggtgcactgg ataattcagg cagtattatt gccaatggtc aaacagatgt
      661 tagtgcgcaa caaggtttaa ataatgcagg acaaatagac attcatcagt taaatgcaaa
      721 aggttcggcg tttgacaatc acaatggaac aattatcagt gatgcggtcc acattcaagc
      781 cggcagcctg aataatcaaa atggcaacat cacaacacgc caacagttag agattgaaac
      841 cgatcaactg gataacgctc atggcaagtt attatcagca gaaatagcgg atttagccgt
      901 ttcaggcagc ctgaacaatc aaaatggcga aatagcgacc aatcaacaac tgattattca
      961 cgatggtcag caatctaccg ctgtcattga taatacgaat ggcacgatac aatcaggccg
     1021 tgatgttgct attcaggcaa aatcgttatc caacaacggc acacttgccg ctgataataa
     1081 actggatatt gcgttacaag atgattttta tgtagaacgc aatatcgtgg cgggcaatga
     1141 attgtcgctc agtacacgag gcagcctgaa aaattcacat actttgcaag caggaaaacg
     1201 cattcggatt aaagcaaata accttgataa tgcagcacaa ggcaacattc aatccggcgg
     1261 tacgacagac attggcacgc agcacaattt aaccaataga ggcttgattg acggacaaca
     1321 aaccaaaatc caagccgggc aaatgaataa tatcggtaca ggtcggattt atggcgacaa
     1381 tatcgctatt gcggctaccc gcttagacaa tcaagatgaa aacggtacag gtgccgccat
     1441 tgcggcacgt gaaaacctga atttaggcat cggacaatta aacaaccgtg aaaacagtct
     1501 gatttacagc ggtaacgata tggcggttgg cggcgcatta gataccaatg gccaagccac
     1561 aggcaaagcc caaaggatac acaatgccgg cgcaaccatt gaagctgcag gcaaaatgcg
     1621 tttaggtgta gaaaagctgc acaataccaa tgagcatttg aaaacgcagt tggtagaaac
     1681 agggcgcgag catattgttg attacgaagc atttggacga cacgaattat tgcgagaagg
     1741 cacgcaacat gaattaggct ggtctgtcta taacgatgaa tcagaccact tacgcacccc
     1801 tgatggagcg gcgcatgaaa attggcataa atacgattat gaaaaagtca cccaaaaaac
     1861 ccaagttacc caaactgcgc cagccaaaat catttcaggt aatgatttaa ccattgatgg
     1921 taaagaagta tttaataccg atagccaaat cattgctggt ggcaatctca ttgtacaaac
     1981 agaaaaagac ggtttgcata acgagcaaac ctttggcgaa aagaaagtat tcagtgaaaa
     2041 tggcaaatta cacagctatt ggcgtgagaa acataaagga cgagactcaa cgggacatag
     2101 cgaacaaaat tacactttgc cggaggaaat cacacgcaac atttcactgg gttcatttgc
     2161 ctatgaatcg catcgcaaag cattaagcca tcatgcgccc agccaaggca ctgagttgcc
     2221 gcaaagcaac ggtatttcgc taccctatac gtccaattct tttaccccat tacccagcag
     2281 cagcttatac attatcaatc ctgtcaataa aggctatctt gttgaaaccg atccacgctt
     2341 tgccaactac cgtcaatggt tgggtagtga ctatatgctg gacagcctca aactagaccc
     2401 aaacaattta cataaacgtt tgggtgatgg ttattacgag caacgtttaa tcaatgaaca
     2461 aatcgcagag ctgacagggc atcgtcgttt agacggttat caaaacgacg aagaacaatt
     2521 taaagcctta atggataatg gcgcgactgc ggcacgttcg atgaatctca gcgttggcat
     2581 tgcattaagt gccgagcaag tagcgcaact gaccagcgat attgtttggt tggtacaaaa
     2641 agaagttaag cttcctgatg gcggcacaca aaccgtattg gtgccacagg tttatgtacg
     2701 cgttaaaaat ggcgacatag acggtaaagg tgcattgttg tcaggcagca atacacaaat
     2761 caatgtttca ggcagcctga aaaactcagg cacgattgca gggcgcaatg cgcttattat
     2821 caataccgat acgctagaca atatcggtgg gcgtattcat gcgcaaaaat cagcggttac
     2881 ggccacacaa gacatcaata atattggcgg catgctttct gccgaacaga cattattgct
     2941 caacgcaggc aacaacatca acagccaaag caccaccgcc agcagtcaaa atacacaagg
     3001 cagcagcacc tacctagacc gaatggcagg tatttatatc acaggcaaag aaaaaggtgt
     3061 tttagcagcg caggcaggaa aagacatcaa catcattgcc ggtcaaatca gcaatcaatc
     3121 agagcaaggg caaacccggc tgcaagcagg gcgcgacatt aacctagata cggtacaaac
     3181 cagcaaacat caagcaaccc attttgatgc cgataaccat gttattcgcg gttcaacgaa
     3241 cgaagtcggc agcagcattc aaacaaaagg cgatgttacc ctattgtcag ggaataacct
     3301 caatgccaaa gctgccgaag tcagcagcgc aaacggtaca ctcgctgtgt ctgccaaaaa
     3361 tgacatcaac atcagcgcag gcatcaacac gacccatgtt gatgatgcgt ccaaacacac
     3421 aggcagaagc ggtggtggca ataaattagt cattaccgat aaagcccaaa gtcatcacga
     3481 aaccgcccaa agcagcacct ttgaaggcaa gcaagttgta ttgcaggcag gaaacgatgc
     3541 caacatcctt ggcagcaatg ttatttccga taatggcacc cagattcaag caggcaatca
     3601 tgttcgcatt ggtacaaccc aaactcaaag ccaaagcgaa acctatcatc aaacccagaa
     3661 atcaggattg atgagtgcag gtatcggctt cactattggc agcaagacaa acacacaaga
     3721 aaaccaatcc caaagcaacg aacatacagg cagtaccgta ggcagcttga aaggcgatac
     3781 caccattgtt gcaggcaaac actacgaaca aatcggcagt accgtttcca gcccggaagg
     3841 caacaatacc atctatgccc aaagcataga cattcaagcg gcacacaaca aattaaacag
     3901 taataccacc caaacctatg aacaaaaagg cctaacggtg gcattcagtt cgcccgttac
     3961 cgatttggca caacaagcga ttgccgtagc acaaagcagc aaacaagtcg gacaaagcaa
     4021 aaacgaccgc gttaatgcca tggcggctgc caatgcaggc tggcaagcct atcaaacagg
     4081 taagagtgca caaaacttag ccaatggtac aaccaatgcc aaacaagtca gcatctccat
     4141 aacctacggc gaacagcaaa accgacaaac cacccaagtt caagccaatc aagcccaagc
     4201 gagtcaaatt caagcaggtg gtaaaaccac attaatcgcc acaggcgcag cagaacaatc
     4261 caatatcaac atcgcaggct cagatgttgc cggcaaagca ggcacaatcc tgattgccga
     4321 taacgacatc acactccaat cagccgagca aagcaatacc gaacgcggcc aaaacaaatc
     4381 ggcaggctgg aacgcaggtg ctgccgtatc attcggacaa ggaggctggt cattaggcgt
     4441 taccgcaggc ggcaatgtcg gcaaaggcta cggcaatggc gacagcatca cccaccgcca
     4501 tagccatatc ggcgacaaag gcagccaaac ccttatccaa agcggtggcg acactaccat
     4561 caaaggcgcg caagtacgcg gcaaaggcgt acaagtcaat gccaaaaacc taagtattca
     4621 aagcgtacaa gatagagaaa cctatcaaag caaacaacaa aacgccagtg cacaagttac
     4681 cgtaggttat ggcttcagtg ccggtggcga ttacagccaa agcaaaatcc gagccgacca
     4741 tgtttcagta accgagcaaa gcggtattta tgccggagaa gacggctatc aaatcaaggt
     4801 cggaaaccat acagacctca aaggcggcat catcaccagt acccaaagcg cagaagacaa
     4861 gggtaaaaac cgctttcaga cggccaccct cacccatagc gacatcaaaa accacagcca
     4921 atacaaaggc gaaagttttg gattgggcgc aagtgcgtcc ataagcggca aaacactggg
     4981 acagggcgca caaaataaac ctcaaaacaa acacctgaca agcgtagccg ataaaaacag
     5041 cgcaagttca tcagtgggtt atggcagcga cagcgacagt caaagcagca tcacaaaaag
     5101 cggcatcaac acccgcaaca ttcaaatcac cgacgaagcc gcacaaatcc ggctgacagg
     5161 caaaacagcg gcacaaacca aagccgatat tgatacaaac gtaaccacag acaccgccga
     5221 acgacattcg ggcagcttga agaacacctt caacaaagaa gcggtgcaaa gtgaactgga
     5281 tttacaaaga accgtcagcc aagattttag taaaaatgtt caacaagcca ataccgagat
     5341 taaccaacat ttagacaaac tcaaagcaga caaagaagca gccgaaacag cagcagccga
     5401 ggcattagcc aatggcgata tggaaactgc caaacgcaaa gcccatgaag ctcaagatgc
     5461 ggcagcaaaa gcagataatt ggcaacaagg caaagtcatt ctcaacatgt tagcctcagg
     5521 tttagctgcg ccgacccaaa gcggagcggg catcgctgcg gctaccgtat cgccagccgt
     5581 atcgtatgcg attggacagc actttaaaga tttagccggt caaaacgcga atggtaaact
     5641 aaccgccagt caagaaaccg cacacgttct tgcccacgcg gtattaggag cagcggttgc
     5701 cgcagtagga gacaacaatg ctctagcagg agcattgagt gcgggcgggt cggaagcggc
     5761 tgcgccttac atcagcaaat ggttatacgg caaagaaaaa ggaagcgact taacggcgga
     5821 agagaaagag actgtaacag cgattacaaa tgtattgggt acggctacgg gtgcggcagt
     5881 cggcaacagc gcaacagatg cagcgcaagg cagcctgaat gcgcaaagtg cggtggagaa
     5941 taatgatact gtagagcaag tgaaatttgc tcttaggcac cctagaattg ctattgcaat
     6001 tggatctgta cataaagatc ctggctctac attagagcct aatatttcaa caattgcttc
     6061 aacttttcaa ttaaatttat ttcctaatag tgaatttggt ggtgaaggtg gagttggcaa
     6121 tgcattcagg cacgttttat ggcaagcaac catcacacga gaatttggca aagatattgc
     6181 tgttaaagta ggaaatagtc atgaaagtgg ggaaaaaatt aattattcta taagacgtaa
     6241 tctttcatta gataaagcag atgaaatgat tgatcaacta aataacgaaa taggaagaga
     6301 aatagcatta aataccaata ggttaaacac aaaagagtta gttggattaa ttctggaaac
     6361 ttataaaaat aatggttttt atcaagcaga aagaaacagt aatggaaatt atgatgttgt
     6421 aagaaaaaga ttatctgaaa aagattacca gaatacaagc aatatattga ttcacttaga
     6481 taatactggt gccggattta aaattcagca gaggagaaaa caaatcagag cacaaatttc
     6541 agccagacaa tggagaagat aaaagatgaa taaaaatata ctttatatat tttctttatt
     6601 aatcactatt gttattttct ttatatttga aaagaatgta ataagaaaaa taagttttaa
     6661 ttataataaa aaagaattct taattagtga tataacgaat tttaattggg attatgtaaa
     6721 actgtatata atcaattcag atttccaaaa aattgttttt tatcataaaa gtaagatagt
     6781 ttttgaggaa ttaatagaac tagataagga agggaatgtt ttacctcaat atctatttga
     6841 ttcagatttg aaaaatgttg aatattacga atgtgattac aagaatggca agatgcaact
     6901 tttaaaaaaa gaaaaatcac atttttttga tggatatttt tattattata aacctataaa
     6961 ttgtaggccg aaacttttgt aaaactatcc ttcgtcatta ccgcctagga ttcaggatta
     7021 ggactcgttt ggggagcagg gagttctgat atggcttgga gtatgtttgc cacaacccaa
     7081 gccgatagag cggtaaggtc tgcaactgca cctaaagaaa tgtggttcca taagaagata
     7141 atagatgaaa aaacaggtaa agtatccttt gataccagac aaatttggtc attgaatgat
     7201 ttaagcaagg aagaactggc aagcattcaa gacacaaatg gcaaagttat tactgtgtct
     7261 aatcctggta ttttcaataa tcgagaagat tcattaagca acgcagcaaa acaaaatcgt
     7321 aatagtacaa acggtagtgg tgttattgca gtcatgaatc ctccaacagg gaaatataaa
     7381 tctgattcta ataacaaaat aaaagatttt ttatggctcg gttcaagtct tgtttctgaa
     7441 ctgatgtatg tcggttacga ccaattaaat aataaagtgt tccaaggcta tttacccaaa
     7501 accaattcag aaaaactgaa tcaagatatt tatcgagagg ttcaaaaaat gggtaacggc
     7561 tggtcggttg ataccagtaa tcacagtcgt gggggaatta cagcaagcgt ttccttaaaa
     7621 gattgggtaa acaatcaaaa acaaaatggc attgccccaa tcagaaaagc acgtttctat
     7681 ggtacagcca caaatgtgca gaatgattac gccgatgttt tacagaaaaa cggctatacc
     7741 tatacgggtg cagacggcaa aacttataac agcggatcct actcaatcgt gcatgataaa
     7801 gattttgtgg ggaacaaatg gatacctttc ttgctaggaa ccaatgacac cacacaaggt
     7861 acatgtaagg ggttgtgcta ttcgcatagc agttattttg cggaggtgcc aaaagcaggt
     7921 acaaaagaat ttgatgacta tgtaaaaata tggggtgaag ttgaatatga cgctcaaggt
     7981 aagccaatta acaaatctaa acccatactg gtagaaccaa acaaaacaaa agataatgaa
     8041 aaatatgaaa aagaagcttt ctaaatattc tctgtttctt tcatctgttt tttgtctaac
     8101 agcttgcgct actaccttat taggtactgt cgtagtcggt acagccttgt acggtactag
     8161 tagtaactgg acaactactg ataaggaaca ccaagaaatt atgaattgcc ttgataaggc
     8221 attagtaaaa ctgaatatct attttgagaa tgatgaagaa aagtacaaaa acaaaacagt
     8281 aatggatatt tactatcagt gcattaaaaa tcctaattat gttgtaacac acaataactt
     8341 ataaaggaga gagatatgaa gaaaaccctt tctaatttag tgctaatatc attctgctca
     8401 acaatgctaa cagcttgccc tttttggggt agttgtgcag actggaattg ctccaaactt
     8461 aatccacgct gggagaaagc cgttgacaca tgtgaaactg aaaatctcac ctttgttttg
     8521 catgaattag gcaatcaatt aggatataaa tctattaccg aagtctcatt tagtgaaatt
     8581 gggcaacata tttcttatca gtgtattcac catggcggtg gatacaatat tcgtaaagat
     8641 aagcagtatg gtcatttgtt tgtgcaataa ccagcgtagc ctgtgcaatt aatgcgaact
     8701 gttacatggt agggtgggca actcgttgcc cacgcagttc agtctcatgt gctaaaacta
     8761 tgttttaacg tgaaatattt catttcaggc tgaaaccatt ttttgtttta accaacacga
     8821 accctaactt aaaatatgcc catcactcca cccttaaaca tcatctctcc taaactctac
     8881 cccaatgaac aatggaacga aagcgaagca ctcggtgcca tcacttggct atggtatcag
     8941 tcgcctacgc atcgccaagt acctattgtg gagatgatga cgtatatatt gcctgtgtta
     9001 aaaaacgggc agttcgcttt gttttgcaag ggtacccaac caatcggtta tatctcatgg
     9061 gcttattttg atgaagtggc gcaggcgcat tatttagaat ctgaccgcca tttgcgtgac
     9121 aacagcgatt ggaactgtgg cgacaatatt tggctgattc aatggtttgc gccattggga
     9181 cacagtcatc aaatgcgctc agctgtgcgc cagttatttc ctagtacgac agtacgcgcc
     9241 ttgtatcata aagggagcga taagggtttg agaattttaa cttttaaaac ttgatgcaat
     9301 cgtgtattga atgcccattg atgattttat tgattttgcg accatgccat gaaacttcct
     9361 ttatcctatt tgcctaatat tcgctttttg tcttggtgct gcttattggc aggtatcatt
     9421 gctcctgcta ctttgttggc ctcccccaac cctgccgaaa tccgtatgca gcaagatatt
     9481 cagcaacgcc aacgcgaaga gcagttgcgc caaaccatgc agcctgaaag cgatgtgcgt
     9541 ttgcatcaaa aaaacacggg ggaaacggtt aatcagttga tgggcgatga cagcagccaa
     9601 ccgtgttttg ccattaacga agtggtgttg gaaggcgaac accatgctcg gtttcagttt
     9661 gccctaaaac gtgccttgcg cgaaacgggt tttcaggctg gcaagtgtct gcatgcgggc
     9721 aacattaatc aaatcatgtc cttagcacaa aatgctttga tcggcagggg atataccacg
     9781 acccgtatct tggctgcgcc acaggatttg aatagtggca agcttcaatt aaccctgata
     9841 ccgagctatc tgcgctccat acgaatcgat cggtctaacg atgatcaaac ccatgcagga
     9901 cgtattgcag cattccagaa caaatttccc acccgctcga acgatctgtt gaatctgcgt
     9961 gatttggaac aaggactgga aaatctcaaa cgtctcccga ctgcggaagc cgatctccaa
    10021 atcgttcccg tagagggaga accaaaccaa agtgatgtcg tggtgcaatg gcggcaacgt
    10081 ctgctgccct accgtgtgag tgtggggatg gataattcgg gtagtgaggc gacaggaaaa
    10141 taccaaggaa atatcacttt ctctgccgac aatcctttgg gactgagtga tatgttctat
    10201 gtaaattatg gacgttcgat tggcggtacg cccgatgagg aaagttttga cggccatcgc
    10261 aaagaaggcg gatcaaacaa ttacgccgta cattattcag cccctttcgg taaatggaca
    10321 tgggcattca atcacaatgg ctaccgttac catcaggcag tttccggatt atcggaagtc
    10381 tatgactata atggaaaaag ttacaatact gatttcggct tcaaccgcct gttgtatcgt
    10441 gatgccaaac gcaaaaccta tctcggtgta aaactgtgga tgagggaaac aaaaagttac
    10501 attgatgatg ccgaactgac tgtacaacgg cgtaaaactg cgggttggtt ggcagaactt
    10561 tcccacaaag aatatatcgg tcgcagtacg gcagatttta agttgaaata taaacgcggc
    10621 accggcatga aagatgctct gcgcgcgcct gaagaagcct ttggcgaagg cacgtcacgt
    10681 atgaaaattt ggacggcatc ggctgatgta aatactcctt ttcaaatcgg taaacagcta
    10741 tttgcctatg acacatccgt tcatgcacaa tggaacaaaa ccccgctaac atcgcaagac
    10801 aaactggcta tcggcggaca ccacaccgta cgtggcttcg acggtgaaat gagtttgtct
    10861 gccgagcggg gatggtattg gcgcaacgat ttgagctggc aatttaaacc aggccatcag
    10921 ctttatcttg gggctgatgt aggacatgtt tcaggacaat ccgccaaatg gttatcgggc
    10981 caaactctag tcggcacagc aattgggata cgcgggcaga taaagcttgg cggcaacctg
    11041 cattacgata tatttaccgg ccgcgcattg aaaaagcccg aatttttcca atcaaggaaa
    11101 tgggcaagcg gttttcaggt aggctatacg ttttaaaacg gcatagtcaa atcaacggta
    11161 actatagata tagcttttca taataaaaca cctatcatga ttatggtaag agatggctgt
    11221 gttttgatat tagattgaca caggaattta agaaggaatc atgaaataca tgattccttc
    11281 tcgtttgcct acattaaaca tataggccgc caactcaatc aatagagtga tttaccagtt
    11341 ttccaatctt cccagtatat ctcctaccga atacgattct tctaatttct cggtttcaaa
    11401 caccataaga tgaagtgatt ttcagggtga aagtcgaccg gccatctctg acagcggcag
    11461 ctaccggaat gtaaacggta gaccttcttc attttcatcc gggataaaat gccacttctg
    11521 cgccaaccat tcattctcac gcaatacatc cgagtattta acaagaacct gttctttagc
    11581 ttgaacgatt gtatctatca taaaggatgc atcttctctt cccaaatcgc aatatttctc
    11641 agcaaaatcc aacaatgtat tcttggaagg atatgttttc ttaccgtggt tagtcaggtt
    11701 taatgccagc gtatcgtcat aagcatcaaa aattccttgt gttccaaccc tgtatattga
    11761 tgtatccaat acatcataga caggtgcaag acgaacatcg tattcgtcat gatagagtac
    11821 tgaaaaattt ttgaggtgtg catcgccgtt tttcaatatg caactggcag caagctgatt
    11881 aaagaaatgg attaaatctt catctggtct gccggatatc tgtcggataa tctgtgcaat
    11941 agccgcataa ctgcctttat atttatcttc taccgaatac tggcgcagac tggtaaagtc
    12001 ttccatccct aaaaaataac cctgttcact gacatcaaac cgacgtacca ataagactga
    12061 tgaatcttcc gacaggctgg tctgtgcaac ggcaatgccg gcttgtttga tggtctgcat
    12121 gcataaaaat tcattggcag ccaagcaagg atattcggat gcatcaaaac ctttggcaat
    12181 atatgaggca gtttgcttgg tatttctgcg gatggcatct aaggacatct tctgctgtat
    12241 cccggataca ctgacgaaac ggccgtgatg gaagatttct gccatatatt gctgaaaaac
    12301 ctgtcgggca tttatgccca gcaaatcccg ttcagtcaat attcttggat ttttcatctc
    12361 caacccgtca atccattcat taaaaagcgg gtcattacag cgcacatgta tccgacccaa
    12421 agtctctctg cacagaattg ccaagcgcag catctcattg tcttcaaaag gcgcatcatg
    12481 aaaagcatat ttgcttgtga tgtgtgcatc caaaaagcct tccggaaaat actgtgcaaa
    12541 gatatgcggc atattgttgc tgatatatac cttgcttctg tcttgataat gcaggcccag
    12601 caacgaagaa ttgggattgt cgtatgcgaa ccgatacatg gccccctttt ccaaagtacc
    12661 gattctttca tcgtttgccc aaacatccaa ataggtgatt ctgggtttac gcatacgatt
    12721 tccggatacg gtgaggatag acttcatcat gaaaaattct ttcaaacggg aacagcggta
    12781 cgtttagaca catctgctga tttaattgta catggcgtat tgcggatgaa attcagtaat
    12841 accgcttctc gtgcctttta tctcgttcgg ttagcattac attattgggt ttttgcgttt
    12901 ccatagctgt aaattctccc attcttgagt attcaagtgc ggttcgattt ggttaagtaa
    12961 gtcataaact gattgcttgt cttcatcgct tggaaaccaa tcatctaaaa tcacccgacc
    13021 gattgagcca gtttgtaaca ctgctgtgta aagataatcc acgcaagcac gggggtgatc
    13081 tgcgagccaa actgggatgt tttcagggaa ataacccatt ttgttcagtc gatccgtccc
    13141 atcaataatg ccattatcac caaataaatg attggtattt cgctcttgat tttcaccgta
    13201 aatataaaat ttttcagggt aagcccgatc actcaatgcc tttgcactat gccagtcgcc
    13261 tgtaccgtta ggactatgca cgtttaaggc tacaatactg ctaacatatc gaacaggttc
    13321 cgttgggggg tatatgtaac ataaagattc cttaaaattt agcagagcaa aagtggtttc
    13381 cacgttttag gacatatttt acaggatagt caccccaata atgtaaggct gtgttttagt
    13441 aatctgttga tttcaattac ttgcaaggga aaagacaatt attttccggt taggaataaa
    13501 cctatcctgt tgaatacctt aaagccaaac acgcctatca acatcatatt aaaacacagc
    13561 caataattaa aagtatcgaa ttttctaacc ctgcttgctt ggaaagcaaa attgctacaa
    13621 tcttggttaa atcagctaca aagatcattt gtagagcaaa aagcaagcag tccgcagctt
    13681 ctttttgaag gtgcggactg tattgatttt gactcggtta aagctgcatt ctgggctagc
    13741 aaactatttt ttttctaacc tttccaataa atcctgagga gcaacatact ctaaagcatt
    13801 tcctgatttg aacggcacta ggaatctata ttctcccagt ttactcaaat cgctacgggc
    13861 agtattcagg gagatgccgt attggttggc aatttcttgt gcagtaaaga tttttccgct
    13921 ttcttccact gctttttgca ggataccaat ttgccgttgg ttcaactttc ctatcttttc
    13981 agtatattgg gcaatcgctg ctttgaattc ctgttggtgt ttttgtttgt cggaaatgta
    14041 gtgctccaaa tcggcaaccg cccgcttgat aatatcgcat tggtaataga tgaaataggt
    14101 taaatctaaa tcgtcagttt ccgcatacaa ataggatttg gcgtattggg caggagcgtt
    14161 tttcagaaga cggctgatgg atatgtattc aaatagccag tagccgtttt tgagcataaa
    14221 ccaatagaac aaagcccgcg ctgtccgccc gttgccatca ccaaatgggt ggatgtagcc
    14281 gatgaggaaa tgcaagataa tagcttggac aaccggatgg ataaacggat tttccacgcc
    14341 gtcataggta ttattggcaa acgcacacac ctcttccatc agcgtatgaa cctgtccgtg
    14401 cggcggtggt tgatacaggc tgttaccatt gatatcggcg ataaagattt cgtcatcctg
    14461 cctgaattgt ccgggctcgg ccttgttttc aatagcgtta ctggtagcaa tgcggtgcaa
    14521 atccaaaatc atttcaacac ttaacggcgt atttttcaat tctaccgctt ttttcatcaa
    14581 gtgatagttg ttcactatca tgatttcgtc ttttgttttg ggtttacgct gcgatttgag
    14641 catatccttg gccactttac gcgtggtagc cgcaccttcc agttgggcgg atgtaatcgc
    14701 ttcttccata atcagagact tgagcaagaa tctgttttgc tcgcttctgc cgaagccacc
    14761 caagctagac gtgccgatag aactgccgca gcttttgtca atcaaatgaa gccgtgcctg
    14821 caaagagtcg ggaatgcaga accaaaactg atgttcaaac gggaaatcaa ttggtttttg
    14881 gatttttttg cggctttcct taacggcgcg ccatttcatc cgcgtatctt ccgtgtgaat
    14941 ccggcggaat ttgtcccaat gcaggtaggt tccattttca tctgtcacgg aaaattctga
    15001 ataatctttg atttcagtaa gaaaatcggg aatgtctata tcaggattgt ttgcggaaga
    15061 attcagcagc ttggcaattt gcgtcattct ttcagtgaga tgctgcatat attcctgttg
    15121 caacagggta aattccggag gtctggaaat tttcatgata aataccaaaa tcgcgcaatt
    15181 ttttataatt gagctattct agtcaatttt tatagaaata gaaagcatta aaatacgaat
    15241 ctgaaagaaa cgatcacctt cataaatagg gaaagattgt gtctactata ttaaaaaagg
    15301 ctgtgtttta atatggtgtt gataggcgta tttggcttta aggtattcaa caggataggt
    15361 ttattcctaa ccggaaaata attgtctttt cccttgcaaa taattgaaat caatagatta
    15421 ctaaaacaca gcctaatgta aaaacaaatt ggtacaaatg gcgaagataa catttggctc
    15481 aaacgtaccg agcggctacg cattgaatgg taaaatacct accgcaaatt gaaagctgca
    15541 agacgacagc tctactatac gttcgcaaac agaattgccg aacaaagccg gataatctgt
    15601 atcaaaaaat tgaatctgct caacctacag tgcatacaga ttaaagtcaa caaactccac
    15661 cagaagatag ttgcctcatc cctactgctg catatgctga aagaaggtgc agctaaaaat
    15721 ggagttcata tcgaagagat taaagccaat gctaccagcc aatcatgtta ctcttgtggt
    15781 cgcttgaaca aacggatagg tgttgctgcc acaagaacca atatggtttg tgagagtttt
    15841 ggtcggcatt acgacactga tgaaaatgcc tcctccaata ttcgaaaata gggagctgaa
    15901 aagctaggca catagagtag cagttgaccg taaaatcata aacgcttcgg aataaaaaga
    15961 tgtagtgatc gcatccttgc attccttttt atgttttgtt gctttagcag ttttacagtt
    16021 tagcaactgt acattttggc atttttaact tttcatatac gaaccaacat ttttaaatac
    16081 tgcaaatatt ttgtacaaaa tatattcatt atgccttttt gatttctcat cattaatatc
    16141 tcgtttatat taatattgca aataatttaa acaaatttta ggaaaggaaa ctaattgcgg
    16201 taatatctaa aatccaaaga actgcttctt tcttttttac cttcttgctg gttttatgct
    16261 tcaacagttg tacagtttta ctccatgcaa accaaacaac aaaaaaagac cgacgaagaa
    16321 gtcttctgct tagaatttaa agaatactgg agtaggggac tcccgttttc cgcgacatca
    16381 tttccaaata gacacggggt acgctcagca tttcatctga accatatcca tctttttccc
    16441 cagttttaac cttgtgaaac atccagtcct ttttcaaatc ttcagacaga aaagaaatac
    16501 tttcctgttt gattgacttg aagagaacca tcctaccatc actcttgtaa gaaaaggcag
    16561 tatctaatac ctatcttcta gagtcgatat actcccttga cgaaaaaaat gatgatatta
    16621 cggataccaa aactaaggtc gtatccgccc cccctactct ccctaagcaa agagatgaaa
    16681 cagcgtatcg actccctgcc ggttgaattt tccgaaaaaa cgcgacgtaa ccagcatcaa
    16741 catatataag aacagcacaa atagcatcaa tacatcaggc aacgaaaatg cagaataatg
    16801 cacttaatgg tgtttggata tctgttgttt tgtgctgtta gtaattcttc tttctgtgtt
    16861 tacagtttag cagttgtaca gttttatagt aatgtttaaa caatgactga tttattttaa
    16921 atgcagatat tgtagaggat aaaaatggcc aaagtccttt cagtaacatt tttgattttt
    16981 tagcgagcct tctcatttcc ccagcgagat cggcaatggc agcggtactt tggccgccga
    17041 tatgcttaag ttcagtaacc ttagtgcgta aatccagtaa ccttaagtta cgtaaaccat
    17101 tagctggcag ggtgtgagtt cgatttgagc aagcattatt ttgtctcctg ttgtacggtt
    17161 tcagacggca tcggtttgcc acgtttgtac caagtggcgc gggaaatgcc gagactctcc
    17221 cacggtttat cgcagctgca attttaaact cctaaaataa gtttgccttt tttgtactaa
    17281 ttactgcttg gatagtttag attagactta agtaaatttc gagtgagttg tttcacaccc
    17341 tacccgaagc tctaaaacgc acagaaatcg ccgcagcgcg ccaaaaactg acagagctgc
    17401 ggcggatgtt ggtgagtaaa ggaggttaat ttaatttcag gttaggactt tccaattcaa
    17461 atttctgttt ttccagaacc tgattgattt gctcatcaga caatccagct tctgttaggg
    17521 atatacgaag ttgttcaaca cgttgaagga aagcctgttt ggcttgatac acaccatcag
    17581 cgataaattg tgcgttcaaa tcactttcaa ggcggtaaaa cttaatgttt gaaatacagg
    17641 agtaaaacca atcaaaccga gcggtcagtt cctgaatggc tattgttccg tgtgcttcac
    17701 tgatgccttc acggcctaga taaacactgc cctgaatgtt ctcaaatcca tgtctagcca
    17761 agatggtttt aatatcggag taggcattgg tatagttatt tccgtggtaa ttgtctttca
    17821 ggcagttggt atccatatca aaggtaatca ggtaacggct cattattttt ctcctgttgc
    17881 gtttcagaca gcatcggttt cccgcgatag taccaagtgc ggcggctgat aacgagcttt
    17941 tcccacggtt tatcgcggct gatagtgttt tcggcgaggt agtcggcacg tgtttgagac
    18001 caccagcgag tgattgtgct ttcagctaca ataattttgc tgcttgccct atgtttaaaa
    18061 atctatccat attggatagt ttagattaga cttaagtaga tttcaagtga gctgtttaac
    18121 ccttagctag caagggtttt ggtggcgtaa ggttactgaa cttaagcata tcggcggcca
    18181 aagtaccgct gccattgccg atctcgccgg ggaaatgaga aggctcgcta aaaaatcaaa
    18241 aatgttactg aaaggacttt ggccattttt atcctctaca atatctgcat ttaaaataaa
    18301 tcagtcattg tttaaacatt actgtaaaac tgtacaactg ctaaactgta aacacagaaa
    18361 gaagaattac taacagcaca aaacaacaga tatccaaaca ccattaagtg cattattctg
    18421 cattttcgtt gcctgatgta ttgatgctat ttgtgctgtt cttatatatg ttgatgctgg
    18481 ttacgtcgcg ttttttcgga aaattcaacc ggcagggagc cgatacgctg tttcatctct
    18541 ttgcttaggg agagtagggg ggcggatacg accttagttt tggtatccgt aatatcatca
    18601 tttttttcgt caagggaata tacacggagg atgattcaaa accggcaggc aaaaaaagac
    18661 cgcttctgaa ctgcatccca aaagttggac accttaccga ctttaggggg tgcagttttt
    18721 tatggcaaaa tattcagatg aattccgact tgccgtcgtt caatactatt tggcagggaa
    18781 cagcagacca tctttctatt tccgattcat tggtacgcag atgggtgaca aaatacagat
    18841 tacacggaga gagtggcatc aaacgtagaa agcatacgac aaaatattcg gtcgaataca
    18901 aacttgaggc aatccgcctg gtggcggggt agggaatgtc ccaaaaagct gccgcagacc
    18961 aactgaattt gcccgactgc tccatcttgc tgcaatggtt gcgcctctac catttgaatg
    19021 gtattaacgg tttaaagccc aaaccctaaa cccaaaggaa gaaagcccgt gaaaaaacag
    19081 catccgccgg aaacgaaaaa gccgactatc tgaaaaccaa ggaagaactg cttgcggaat
    19141 tggcttgcct taaagcggaa atggctgccc taaaaaagct cgatgcctta atctatggga
    19201 aagaagtgcg gtagaaagaa cgcaactcgt cgcagggtta aggcaatgcc atccgttgaa
    19261 ctgctgttgg tgattgtcgg actgccacgc ggcaccttct attaccaatt ggttgtccaa
    19321 tcggcagaag acaaatatgc cgatttgaaa cggcatatcc atgatattta tcaaaagcag
    19381 ttgaaagaca acggtctggt tcagagtatg tcccgcaagg gaaactgctt ggacaatgcg
    19441 gcaatggaaa gtttcttcgg aacgttgaaa tcggaatgtt tccatacgtg caaatatgat
    19501 tccgttaccg aattggaagc tgtactgcac gaatatatcc gttactacaa caacgataga
    19561 atcaagttga aattaaaagg actgagccct gttcagtaca gaattcagtc cctgaaagcc
    19621 gcttgattaa actgtccaac ttttgggggt cagttaatat cggttccacc aatagtcgtc
    19681 agggttgtag ttgggggtct gcctgtatgc ctatgccgat aagctgagca cctttgaggg
    19741 tggtatcccc gccgcttcgg atggtggttt gtccggccgt actgccgaca tgggtgtggc
    19801 ggtgggttgc ttttgtctga ttgctgtggt ggcttgttga aaaaaagata gtgtatttca
    19861 cggtcaaata actttacatt tcttgaagtt gcaagaatgc gcaccccgac tttttcggga
    19921 tgtttgtttc gttttgaatg gatttgatgt tttaatttat actttatttt caataaattg
    19981 aaaatcaccg gcgaaagccc agtttaattt tttatttata tctttccact tggaaatctt
    20041 ggccgttttg caaaatcgta aacgctatta ccgccagttt ccgcatgatg gcaattaata
    20101 tcaattttat atgcttccct ttatttttca gacgccctac aaattcaggg aaggcattac
    20161 aacgatatgc aacaagtgca ggcatataaa ggcttttcct tatttccgaa cttcctattt
    20221 ttgatattct gctttttccg ttcacgcttg ttcctgattg aaattttcta gggtctaggc
    20281 ctagataagc cgtgaactgt cttgcatttt taaattcatg tcttttatag gttgatagca
    20341 atactgctgt cgcttgctcg cctatgcctg ttattgtttt cagccttttg cgtagattgt
    20401 tataacttgg attgtctttg tagaactgga gtaattgctt tttgactatc tgtatttgtg
    20461 ctgtcaggtt tgaaatagtt gtttgaatat gggatttgat atagtcgggt gcttcgtgtt
    20521 gtttagcttt ttctgttgcg cgttgctgtt tcagatagtc taaatatcgg gcgatttcct
    20581 gtaattgctt ctgttctttt gtcggcggtt tccatgcttt taatttgtgc tttcggtctt
    20641 ggcaatattg ggctatcaac tttgcgtctt gtgtatctgt ttttgatcgt tgtagttctg
    20701 ctatcgcata tccttttatc tttcgtggat tctctacggt aattgtatat cttgaataaa
    20761 gatattcggc taatgcttcg taatatgtgc ctgttgcttc gcacacgcaa tggagcttat
    20821 cgtttacttt atgactttgt agccaattta ttaattgttc aaatcctcct ttgttgttct
    20881 gaaacttctt ttgataattt tgaccgtcta caatcaaaca gcaatctatt gtgagctttg
    20941 aaacgtctat tcctaagtac atggtttaac cttattaatt cgggcttttt gccctagata
    21001 gtgttcaaac ttaagatgta cgaaagccca cgcttctatc tttcttacaa gctgtacgct
    21061 ttggccgtac tttcgaagtc gtgggcttta cttggtgttt cgtcaaacgc caagccctca
    21121 atgggctgat ttactcattc agggcttgaa gcttatcgcc tttgccgtat ctgattttat
    21181 tggggtcgca ggttttggta aaggtttctg ttgcgacccg aatgtctgat tttttttggg
    21241 cgtatctcag tctggaatca ctccgttcgg gggtttccgg tattgaaaaa cagttcataa
    21301 aaaaggaaaa gggggtattc ataaagattg ggtaaaaagc gcgcccaatc tttacaaagc
    21361 ttcccccttt tccttttttc tgccctattt tcctgcacct acaacccccg aacgaagcga
    21421 ttccagactg agacacttta aaaaaaacag ccattctagc aattaacccc cttcactcag
    21481 ctcaagccat cctgaggggt agggattgaa ctttctgctt tacgactccg ccgccaattc
    21541 cttcaaacgg ttttccgcgc tcttcagttg tcgtacatga gattttgcta cggctttccg
    21601 cccattacga gaacttgcgg cttgtccgct ttcgcggact gttccacctg ttccgtcctt
    21661 tgctgttcgt ccttataagg attgaaaggc aacccgtttt tcacatattc tttacacatt
    21721 atctttgtta tttctttcaa gggtgttcct tgatttgaat agcatgtgca atctgatttt
    21781 ccgccgtcta tacatccggc gatttgctca aaggttttta cttgtcggac tgtgttataa
    21841 ataggcttgc tttcgggctt ttcgggtaaa gtcggcacaa agtcttcagg tttcaaattg
    21901 tctgaatttt ttaaaggcat ttcctctgat gatgcaggct gctccgtcat cgtctgcaca
    21961 acgctttctt tttgcgcttc ctgctcaatc cggctgtctg tggctttgct gtaaacttta
    22021 aaaatgccgt aacttttcca gcctacaaac cctacaatcg caatcaacgc ccaaaccgcc
    22081 caaggtactt ttttcttgaa cttttggtgc tggcttgctg atttatagta tttaaaggct
    22141 tctttcggcg gtttccaact tgcgacttct acgccgctta cgcctgcggg attgtccaac
    22201 gaggttacgc atttatacca ataatactgt ttcattccga ttgccttgcg ttcaaggtgt
    22261 acatgctttg aaacaaggtt gcggacgaat atatcaagtt ggctcgggtg ctgcgtcatc
    22321 aaaataacgg tatgcccgtg atggcggagt tctgtcagtt cctgaatata aggcggaacg
    22381 ggacggcctg ccgcgcgtac cggataagtg tagtgcgctt cgtcaacaat cagcactgcg
    22441 ccttccggta tgacatcacg aagcggggcg gacatgattt gctcttccgt cagttcgtgg
    22501 gctttaaact gccgtttatc caatccgtcg atatggcaga aataaagcgg tctgtctacc
    22561 tctgtgccgt cttccaattt cattttgaac aatccgtctt cgttgttcaa aatcatagag
    22621 acgacgcggg aggttttgcc tgtccccatg tttcctgtaa acagataaat catgcttcta
    22681 cctcatcctg gaaagacaaa cgtcagtttt ttgaatgcgt gcataccaat gaagaacgag
    22741 aatgcgccga acaggtagcc caacccctga ccgaatcccg aaattaaaag aaggttcagt
    22801 atgtcggaag gcatggaatt gatcgcattt gacgtgtagt ctttgaactt ttccagcgcg
    22861 atgagatacc cggcataggt tacaaatgtc agacctgttg caaggattat tctgacaatc
    22921 agcattttca gaagtatgcc taaaagtgga atcagaccgg caagtaatgg catttatttc
    22981 cccttcaacg aaccgaaaac gacaaaagcc gacataatga taaaggcgag cagtacggca
    23041 aaacggattt tttcggcaaa cacgcataac ggctcatagc ttgcctgata ttgcctaccg
    23101 aaaacatgaa aggttttcgg ctgcggacat acgccgttag acggtaaaaa gttatgtgaa
    23161 gaccatgttt tatcgtctat aacctgcggt atgcttatat cgtgaaacat gccgtctgaa
    23221 ggtttgccca tctcctgaca ggctaggatt tccggaaaat aatcgcacaa aagcccgccg
    23281 tcttcgcctt ctttcctttc ctttctttgc gatgcctacc gtttgggcgg tccggaac
//
LOCUS       DAWIVB010000012        22675 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWIVB010000012 DAWIVB010000000
VERSION     DAWIVB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.012"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(120..1034)
                     /locus_tag="WIX60_000328"
     CDS             complement(120..1034)
                     /locus_tag="WIX60_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ5304652.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WIX60_000329"
     CDS             1290..2759
                     /locus_tag="WIX60_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ5304653.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WIX60_000330"
     CDS             3011..3382
                     /locus_tag="WIX60_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304654.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WIX60_000331"
     CDS             3455..4864
                     /locus_tag="WIX60_000331"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ5304655.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPA
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGEMVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WIX60_000332"
     CDS             4910..5704
                     /locus_tag="WIX60_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304656.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDAQIRQEAEAGKSDAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="WIX60_000333"
     CDS             5892..6668
                     /locus_tag="WIX60_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ5304657.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6744..6850
                     /locus_tag="WIX60_000334"
                     /pseudo
     CDS             6744..6850
                     /locus_tag="WIX60_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6921..7064)
                     /locus_tag="WIX60_000335"
     CDS             complement(6921..7064)
                     /locus_tag="WIX60_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304658.1"
                     /translation="MENNKVKSDYAAKQESLGKVDENKNETMKIPPQVGTHMLGFSFI
                     FKC"
     gene            complement(7739..10210)
                     /locus_tag="WIX60_000336"
     CDS             complement(7739..10210)
                     /locus_tag="WIX60_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ5304659.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGSDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRVSSGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LVVLGNAMRLHKQVDMPSEKMPSEQ"
     gene            complement(10207..11385)
                     /gene="hflX"
                     /locus_tag="WIX60_000337"
     CDS             complement(10207..11385)
                     /gene="hflX"
                     /locus_tag="WIX60_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ5304660.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11390..12670)
                     /locus_tag="WIX60_000338"
     CDS             complement(11390..12670)
                     /locus_tag="WIX60_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ5304661.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12823..13548)
                     /locus_tag="WIX60_000339"
     CDS             complement(12823..13548)
                     /locus_tag="WIX60_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ5304662.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13621..14298)
                     /gene="radC"
                     /locus_tag="WIX60_000340"
     CDS             complement(13621..14298)
                     /gene="radC"
                     /locus_tag="WIX60_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ5304663.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14421..15770)
                     /gene="gshA"
                     /locus_tag="WIX60_000341"
     CDS             complement(14421..15770)
                     /gene="gshA"
                     /locus_tag="WIX60_000341"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ5304664.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            16030..17439
                     /gene="leuC"
                     /locus_tag="WIX60_000342"
     CDS             16030..17439
                     /gene="leuC"
                     /locus_tag="WIX60_000342"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ5304665.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17536..17790
                     /locus_tag="WIX60_000343"
     CDS             17536..17790
                     /locus_tag="WIX60_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304666.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17852..18493
                     /gene="leuD"
                     /locus_tag="WIX60_000344"
     CDS             17852..18493
                     /gene="leuD"
                     /locus_tag="WIX60_000344"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ5304667.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEERVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18668..18928
                     /gene="dcm"
                     /locus_tag="WIX60_000345"
     CDS             18668..18928
                     /gene="dcm"
                     /locus_tag="WIX60_000345"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ5304668.1"
                     /translation="MQQIKFIDLFSEMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFA"
     gene            18974..19489
                     /gene="dcm"
                     /locus_tag="WIX60_000346"
     CDS             18974..19489
                     /gene="dcm"
                     /locus_tag="WIX60_000346"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ5304669.1"
                     /translation="MARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETL
                     EALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPT
                     ESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKE
                     RRKKNGLQKSA"
     gene            19504..19938
                     /locus_tag="WIX60_000347"
     CDS             19504..19938
                     /locus_tag="WIX60_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ5304670.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDQNDVAPTLVAMNMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            19954..20115
                     /locus_tag="WIX60_000348"
     CDS             19954..20115
                     /locus_tag="WIX60_000348"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ5304671.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            20116..20685
                     /locus_tag="WIX60_000349"
     CDS             20116..20685
                     /locus_tag="WIX60_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ5304672.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPG
                     FDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20719..21789
                     /gene="leuB"
                     /locus_tag="WIX60_000350"
     CDS             20719..21789
                     /gene="leuB"
                     /locus_tag="WIX60_000350"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ5304673.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDASYEYAPLGG
                     EAYDEYGHPYPQFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAILYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21952..22515
                     /locus_tag="WIX60_000351"
     CDS             21952..22515
                     /locus_tag="WIX60_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ5304674.1"
                     /translation="MKKIIIAALAAAAIGTTSAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPNIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     QWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5534 a         5917 c         5815 g         5409 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc agcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggttg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggctccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcgaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaagggcgt gaacgtattt atgttgtcgc ccgacggtaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgagaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaactcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaatgtt tgcgcgggac acattgccaa
     4261 acaaatgggc ctgcctatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggtg cataccgccc gcgcaacagc gcgcatactt atgtaacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctgcggaaa tcaatacgct gtgggcggaa gtggctgcag gcaaaggctt
     4501 tgacctgcga tttgccttgg acaaagtcgg tggcaaatac ggttttacct ctggcaaatc
     4561 cacccacgcc gaccgcctcg ccaccatcaa gcaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggtgaaat
     4681 ggtggtttgt ttggaaaccg cgttggcggc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac aggaggcagc
     4981 catcgccccc cacctgcttg ccgacggaac caaggtatgg atacgcaaag caggcaggca
     5041 caatgcacga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgctcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatgcg caaatccgcc aagaagccga
     5281 ggctggaaag tcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcacg aaatatgatg tgggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg atccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacattc attctaccgc gctaatattg aaaaacggcg gactgctgga
     5521 agctgctgcg gaaaaatggg gcggcgtatt gtcggatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 ataagccgcc gcccgcccca cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttgacttta tcccgaattg
     5821 ggcagatatt ctcattctga cacacgtccc aaataaacac tccccatttc ttttaccgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggccaat
     6001 tcgttatggt cgggctgatg gtggacggca agccactgat gcgcgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaaccttggt tgccggcgac ctgaatccgg gcaaacacct ttacctgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagatcccga aatttacgaa cagtttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gtgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcgcgcgaa gaattcgaac accacggccg cctgaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaattaa tcctcaagac gaccgcgcca
     6541 tgctgtgcgg cagccccgcg atgctgaaag acacctgcaa agttttggac gatttcggac
     6601 tgaccgtctc tccgaaaaca ggcgtacgcg gcgattacct gattgagcgc gcatttgtgg
     6661 accaataatc tgcttgaaaa tgccgtctga acggcatctg cgataaaaaa ccccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg aattaaattt aaattaggac aaggcggcga
     6781 gccgcagaca gtacaaatag tacggcaagg cgagccaaca ctgtaccggt ttaaatttaa
     6841 ttcactatat agtgggtaaa aattagaaat gcacatttcc gtcattcacg cgcaagtggg
     6901 aatccagacc tatccgttaa ttagcatttg aaaataaaag aaaaaccaag catatgggtt
     6961 cccacttgcg ggggaatctt catcgtctca tttttgtttt catcaacttt tcctaaactt
     7021 tcctgtttgg ctgcataatc agacttgact ttgttatttt ccatattgag cttctcttaa
     7081 tttgaaaaaa ttaacataaa catttaaaaa taaaacaaaa ttatataata aaaaaataat
     7141 gagaatataa aggataaaaa gagagttaag ttatttaatc ggacttgttc tctataaatc
     7201 ataagacacc aaataagcca taataaattt agtaaataaa aactgcttat acccaacttt
     7261 cttttcttgg aacctattat tatttcaact ccggatgaaa aaatagaatt taaaaatttt
     7321 tttctttgat atatcgttaa tatctcccta gtttttctta aaaaaatata atcatatata
     7381 tactacacaa cattggtaat aaataaatta aaagcgaaag gtaattaaat tgataattgt
     7441 aaattatata ataaacaaca tacgaagaaa aaattattat agatgattta ctatacgaat
     7501 aaaaaaatat cctacctagc ccgaaccttc ttataatttg tactatgtga ttatccattt
     7561 caatatccat ttaagctttc aattaatttt ctatattttt caatggcatg tatcatgcta
     7621 tgcttgggaa atatcatctt ggttgatgat caagccatgt atgcaaagat tggaatatgg
     7681 tatttgtcgt gctaataatg cccccgttac tgttatacgt ctaaagcaac gctgtccgtc
     7741 attgttcgga cggcattttt tcagacggca tgtccacctg cttgtgaagc cgcatagcat
     7801 tgcccaatac gaccagcgaa ctgaagctca tacccagtgc ggcaatccac ggttggacat
     7861 agcccaaaac ggcaagcggt acggcaatga tattgtacgc gcccgcccat atcaggtttt
     7921 gccggataat atggcgggtg cgccgcgcct gatcgagcag gtgggcgacg gtacgcaaat
     7981 cttcgttcaa taacacaatg tccgcgccgt ccctcgcaat atccgtcccg cccgctgcgg
     8041 cggcggatac gtctgcctgc gccaaaacgg gcgcgtcgtt gatgccgtcg cctatcatca
     8101 gcactttttt cccttctttt tgcaaggctt tgacgtattc cagtttgtcc tcgggcatgg
     8161 cttgggcgcg gtagtgcgcg acacccaggg cgcgtgcggt ttcggcaacg gcggtttcgc
     8221 ggtcgccgct gaggatgtgc agggtcaggt ttttgcctgc caactgccgc accgcctccg
     8281 ccgcgctgtc tttcaagggg tcggtcaggt agaacacggc ttggaaaccg ctttgactgc
     8341 cgaggtaaac cgcgctgccg ccgccttctg tttgcggttc tttacctgaa atttcggcga
     8401 cataggatgc cctgcccaat gcccaaacct gtgtttcgcc gttgacggtc agttgcgcgc
     8461 ccacgccttc gccgatgcgg ttgaggcgtt gtttaatagc gatgtcgggg acgctgccgc
     8521 ttgaaacacg gcagttgagg atggcgcggg caaggggatg ttcggactgt tgttctaaag
     8581 cctgcgccac cgcgagaaca aaggcttcgt ctgtgcctct caacaatgag atacggcgga
     8641 cggcgggttt gccttgggtc agcgtgccgg ttttgtcgaa gatgatgtcg gtggtttggg
     8701 cgagggtttc gattgcctgc tttccgccga ttaaaatgcc ttcgcgcgcc agcgtaccgg
     8761 tagaagctgc cagcgcggtc ggcgtggcaa gcgataaggc gcaggggcag gtaatgacca
     8821 gcagggcgac ggtaatccac aatgcggtgt gcgcgtcggc gtacagcgtc cagccgatga
     8881 agacggggac ggcaagcagg agttcgccga atatgaaaga cgaggcgtat tgttccgcca
     8941 actcggcagt gcgcggtttt tgcgctaagg cgcggtcgag caggcggacg atgtgcgaca
     9001 gtcgcgtgcc gccgccggtg cggtcggtgc gtataatcag ggggctttgc gtgttgagtg
     9061 tgccggcggt tactttttca gacggcattt tggcgacggg caggctctcg ccggtcagca
     9121 tagattcgtt gacggcactg cttccttcca gcaccgtgcc gtcaacgggg atggtttcgc
     9181 ccggtttgac cagcacgata tcgcccgcct tcaatttgac gacagctgcc tcgcaggttt
     9241 cctgcgtatc ggggtaatcg ggcatatgat ggcaaaacgc aggaatcagc ttcaccagcc
     9301 tctcggcggc atcgcctgcc ttacggcggg caatgtgttc cataaagcgt ccgcccagca
     9361 ggaaaaacag cagcatcgcg atggattcga aatacatccc ctgccccgca tttgtcgcaa
     9421 ggctgtaaac gccggcgata aaggtcatga tgatggcgac ggtaatcggc gtatccatgc
     9481 cgacgcggcg gtttttcaag tcgcgcagcg cgccttgata aaacgggacg gcgcaataga
     9541 atacgacggg cagcaccatt aaaaagccgc cccaatggag gatttgcagg aaatcgggtt
     9601 cgatgtcgct gccgtaaagg taggtcggca gcgcgaacat catcgtctgc atcatcccca
     9661 gcccggcaac ggcgaggcgg acgatgtatt gtttgcgttc tttttggttg gcggcttcga
     9721 ttttttgcgc gtcatagggt gcggcggtgt agcctatctg cctgattttc aacagaatgt
     9781 cggaaaggcg gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg ctgtaattga
     9841 ggtcgatgcg gacgatgccg tctgtacgca aaagctgctg ttcgatcagc cagacgcagg
     9901 cggcgcaggt gatgccgccg agcattaaaa ccgcctcgcg cgtgccgccg tgggtttcca
     9961 caaagtcgga ctggacttcg ggcaggtcgt acaggcggat ttggtcgagg atttcttggg
    10021 gcggcagctc ggttttttgc gcgtcggcgg tgcgttgttt gtaataactg cccaagcccg
    10081 cgtcaataat gctttgtgcg accgcctgac agccggcgca gcaggtttct cggtcttcgc
    10141 cttcgtagcg gacggtcaga tgcaggtttt cgggaacatc cagcccgcag tggaaacagg
    10201 tttttttcat ggcatttcgg tttcgtctgt gtttggtgcg gcggcacaag actcggcaat
    10261 ggcttcgcgc agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga cggcggcaat
    10321 ttttcccgca gcgtcgcgcc atatgcccgt gttttgttct tcagacggca gcaggtcggt
    10381 tttgttgtac accttgatgc acggaatatc gccggcatgg atttcttgca gtacgttttc
    10441 cacgtcttca atctgctgtc cgctgttcgg ggcggcggca tcgacgacgt gcagcagcac
    10501 atcggcttgc gcggtttctt ccagcgtggc ggaaaatgcc gaaatcagtt tgtgcggcaa
    10561 atcactgacg aatccgacgg tatcggtcag gataatgctg cattcgggac tgatgtacag
    10621 ccgccgcgcc gtggtgtcga gtgtggcgaa aagctggtct ttcgcatata tgcccgactt
    10681 ggtcagccgg ttgaacaggc tggatttgcc gacattggta tagccgacca gcgcaaacgt
    10741 tttgattgtg cccgattcgc ggggcttgcg gcgcagggcg cgctgttttt tgaggttgac
    10801 aagctgtttt ttcaaggcat tgatccgatg ggcgatcaat cggcggtcgg tttccagttt
    10861 ggtttcgccg gggcctttca tgccgatacc gccgcgctgg ctctgcagat ggccgtaacc
    10921 gcgtatcaag cgtcccgcca aatggctcaa ttgcgccaac tcgacttgca gcctgccttc
    10981 ctgcgtgcgg gcgcggcggg cgaaaatcgc cagaatcagc cctaccctgt ccaatacgcg
    11041 gcatttcagt tctttttcaa ggttgcgttc ctgcgtgggc gtgagttcgt ggttgaatac
    11101 gaccaaatcg atgccgtctg cggcaactgc ttctgacagc tccgccgcct tgcccgtgcc
    11161 gacaaacagc gcggtgtgcg gacggtcgcg tttggcagtc tccacgcgca cggaatcgcc
    11221 gcccgccgct ttgaccagct cgacagcttc cgccaatgcc gtctgaaaac cgttcagacg
    11281 ggtggcactg ctgcccgtgc catctttgtc caacattacg cccaccagca tgacgcgttc
    11341 gggttgcgct tgggtactgc cgttgcggcc tgttctgcct gtcatgtatt caatcctgta
    11401 aataaagggt tacgcctcgt tccgtttctc cttccggtgc aagcgtgatg cggctgcccg
    11461 ccattccgtc aacgctgaag acgatgccgc ccgcctcgtg ccttcctatg gacaggcggc
    11521 tgccgggtcg ctgccggtag gtataacggg tacacgcttc caaacaccag cgtccgcgcc
    11581 agtggatgaa atatttttcg ccttttctaa ccgcaagtgt ctcgccgccg tttacctcaa
    11641 aataagggta atcgggtttt cgggttctaa actgccattt cgcctgcgct cgccgtccgt
    11701 tgcggacata gtcgaatacc gccgtataaa gcgtgccgta ttccaaaggc ttgagcggaa
    11761 aaagcgcgaa ttggtacgcg gtcagcctgc cgttggggtc gttgccggcg gttaaaaccc
    11821 tgacggggcg gatttcgttt ttaccctgat acagcttgaa acttttcatc gtaattttgc
    11881 ctgccgcctc ggaaaaatca atgctggcag gattgcccgt gatttcatat tccggcacgg
    11941 ggtctggacg ctcgccgtgg aaataaggca gtgcgccgtt gccgacggga taggctgtat
    12001 agagcagctc ctgtgcgggc atggcttcgt cggtgtacac gaccgcaccg ttatggcagg
    12061 cgttgcggta atatttccgt cctgcttccg gctgatttct gccttgggcg caatgccgct
    12121 caaacctgcc gttgccctga ttgaatacga gaacggtttt accgttttcg cgcacaaatg
    12181 ccgctcctgc ctcatccgta tggcggtcaa gtagggaaag gcggtggtag attgcgctca
    12241 ttaatccgtc cacttggcgt tgctgcgtgc ggatgtcgct gtcggacgat tcggcggctt
    12301 cctcttccgt gctgatgttt tcatgcacgc cgttgtagag atacccggca aggcgtgtgc
    12361 gttcggtcag cttttgtgcg gtgtagtgcg gattgtcggg atggtgttcg ccgtgtccgt
    12421 cttcgggatt gagcgtgaga tagcgtgcgt gcctgcgggc ggaattttcc aaaaccggcg
    12481 cgtgtgccag cttgtgcaaa ccgatttggg cgcggatgcg gttgaggtag tgcagcgcat
    12541 cgccgccgtc cctgatttgc ggtgcggacg gatagacaag ttcgcccgcg ggcagggatt
    12601 ggttttgggt atgatagaaa acgccggccg ccgaacccaa caggagcagc cgaataaaaa
    12661 gggatttcat aatgccgtcc tcccgacgcg gcaacggggt gttttatcaa aacggcaaac
    12721 ttgccaatgc ggtttccgat tgcagattgc caaaccggca aacctttccc cgtgccgatg
    12781 ccgaacccga tgccgtctga accttcagac ggcatcgggt gtttatttgt ccacccggat
    12841 gggtgcaggt tcgtattgtg tcgattcgtc gccgtaatac agcacgccga gtttgatggg
    12901 gatgcgtccc tgcgatttgc ggtgggcgtt ggaatccctc agggaatagg cacaaccgca
    12961 atattcctgc tggtagaagt tttcacgttt gctgatttca atcatacgcg cgctgccgcc
    13021 gcctttgcgc cagttgaaat cccaatacac cacatcatcg taaggcgcgg cggcgcggtg
    13081 tccgcagtcg ttgatttgcg ccatattttt ccagcgtgaa atgcccagcg aactggtaaa
    13141 gacggggaac ccgtgttcat gcgcgtattg cgccgccttt tcaaaacgca tatcgaaaca
    13201 catggtgcag cggatgccgc gttccggctc aaactccatg cctttggctt tggcaaacca
    13261 ttctttgcgg tcgttttcgt agtcgtcgtc tttatcgatg aaaggaatgc cgaacttttc
    13321 cgcaaagcgt acgttttcct cttttcggag catatattct ttgtgcggat ggatattggg
    13381 attgtaaaaa taaatggtgt agtcgatgcc ggaggcaagc atggcttcca tcacttcgcc
    13441 gctgcacggg gcgcagcagg aatgcagcaa gactttttta tgtccgccgg gcgggacgag
    13501 tatggggcgg tcaatgtcgg taacggtagg tttgttttgt gtttccatta acttaacaat
    13561 atctgtcgaa tacggttgaa gcggctattt tatcagagcc gccgcatgta aaacagggtg
    13621 tcagggcatc agcccgagct gcctgaacga acggacggtt tgcgccgtta cgataaaatg
    13681 gtcgagcagc gacacatcga ccagcgacat ggcctgtgcc agccgccttg tgaacatgat
    13741 gtcttcctgc gaaggttcgg gcgagccgcc cggatggttg tgcgcaataa tcaggctgtc
    13801 ggcatattcg tccaatgcca gtttgacgat ttcgcggatg taaatcgtgt tttccgcaac
    13861 cgtaccgcgc gacagctctc tgaccgcaat cagttggttt tggcggttca gcagcagcgc
    13921 gacgctgact tcgacttttt cctgccccaa atgaaagcgt aaataatcgg caacggtatc
    13981 cggatcggac aggacgatgc tctcctgcaa ttcttccgcc aatatccgcc gcccgatttc
    14041 cctgactacg gcaaactgtg tgaaacttgc cgtccccatc cctttgtatg ccgacagttt
    14101 gccgacctcc gcgctcatca gcctccccaa actgccgaac tcctgcagca aataacgcgc
    14161 caaatcgacc gcactcattc cgcgcgtgcc gacgcgcagc aggattgcca aaagttcggc
    14221 atcactcaaa gccgccgccc cgcgttccaa cagcttttcc ctaggtcttt cgccttccgg
    14281 ccattgcttg atgctcatgt tttccctttt ttaaatagtc tgagatgccg tctgaaacaa
    14341 atgttcccac acgccaaata tccgaaatgc cgtctgaacc ggatttccgc ttcagacggc
    14401 atctgtattt caaaacagtc ttacgccgtt tcttccaact cgatggacgc cgccagaagc
    14461 gacaggcgtg ccattacgcc gtaaacgtag aggcggttgt gttcgttgtc cacgtcgcag
    14521 tctttttcag agcgcggcat acccagcgag tcccattgtt cgaatacgcg cttgcaggct
    14581 tcgggagctt cttgggagtt gtcgccgttg ccggtgggga tgctgttgga cagcggaaca
    14641 aacaccatac cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg tacgcggaaa
    14701 aagccgccga tgacgaagcg gtccatcata tagacgacgg gttcgcacac cgcgccgttt
    14761 aaggtttcat aagtataaat cccttcttgg acaatcactt cgctgacttc caagccttct
    14821 ttgactttcg ccattttatt gcggttttta cggttcaatc cgcgcacttc gtcggcggat
    14881 ttgacgctca tcacgcccat gccgtaagtg ccggcatcgg ctttgacgat gacgaaaggt
    14941 ttgtcggtaa tgcccgattc gtcgtatttg gcttgaattt tcgccagcac acgttctacc
    15001 gcttccgcca acgcgtcttc gccttcacgc ccttggaagt ccaaaccgcc gattttttca
    15061 aaataagggt taatttgcca ttcgtcgatg ccgattaact tggcaaattc ggcggtaact
    15121 tggttgtacg cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc gtgcaacggc
    15181 ggcaaaacgg tttggctgat gcctttgagg atgtcgggaa tgccggcaga caaatcgttg
    15241 ttcaacaaaa ccacgcaagg cgaaaagccg tctgcaagat ggacgcggtc gcgggtacgc
    15301 aataaaggtt ccaacaggat tttgtcgccc aatgcagttt caaattcggt cggttcggtt
    15361 acttccggat tcaggctgcc caagcgcact tcataccctg ccgaacgcaa aatctcgccg
    15421 agggcgtaaa tgttttgcag gtaaaacgta ttgcgcgtgt ggttttcggg aataatcaat
    15481 acggatttcg ccgtttcgca ggcgcgttgc accgcatctt gcgcggcaac cgccgccagc
    15541 gggataaagt tgggattcag attattgaag ccgccgggga acaaattcat atcgatagac
    15601 gaaattttgt aaccggcatt gcgtatatcg accgaaccgt aaaacggcgg gcggtgtacg
    15661 ctccactgcg tgcggaacca cgcctcgatt ttggcgtgat tggacaggat ttttgcctca
    15721 aacgcctgaa gttgcgccga atgttcgggc gacatcaccg gtaatttcat ctttcgtctg
    15781 cctctgtgtg ttttttgaca agatgcagat gataatgcct ttgaaagaat gacgacaagt
    15841 attctttcgt atcgaatcat gctttagata taactgttta ctgaaatgga caatacgtct
    15901 aattttttcg ttaaaattgt ttgcaaaata acaccggctc aaaaattaga caaaatttgt
    15961 tgcgcggtat aaagaatacg tctaaaatcc gcccaatccg cattaccatt tatccaaaag
    16021 aacaacatca tgacagcaca aaccctctac gacaaacttt ggaacagcca cgtcgtccgc
    16081 gaagaagaag acggcaccgt cctgctctac atcgaccgcc atttggtgca cgaagttacc
    16141 agccctcagg catttgaagg cttgaaaatg gcggggcgca agctgtggcg catcgacagc
    16201 gtcgtctcca ccgccgacca caacaccccg accggcgatt gggacaaagg catccaagac
    16261 ccgatttcca agctgcaagt cgatactttg gacaaaaaca ttaaagagtt tggcgcactc
    16321 gcctattttc cgtttatgga caaaggtcag ggcatcgtac acgttatggg ccccgaacaa
    16381 ggcgcgaccc tgcccggtat gaccgtcgtc tgcggcgact cgcacacttc cacccacggc
    16441 gcattcggcg cactggcgca cggcatcggc acttccgaag tcgagcacac gatggcgacc
    16501 cagtgcatta ccgcgaaaaa atccaaatcc atgctgattg ccgttgacgg caaattaaaa
    16561 gcgggcgtta ccgccaaaga cgtggcactt tacatcatcg ggcaaatcgg cacggcgggc
    16621 ggtacaggct acgccgtcga gtttggcggc gaagccatcc gcagcctttc tatggaaggc
    16681 cgcatgactt tatgcaatat ggcgatcgaa gccggtgcgc gctcaggcat ggttgccgtc
    16741 gaccaaacca ccatcgacta tgtaaaagat aaacccttcg cacccgaagg cgaagcgtgg
    16801 gacaaagccg tcgagtactg gcgcacgctg gtgtctgacg aaggtgcggt attcgacaaa
    16861 gaataccgtt tcaacgccga agacatcgaa ccgcaagtca cttggggtac ctcgcctgaa
    16921 atggttttag acatcagcag caaagtgccg aatcctgccg aagaaaccga tccggtcaaa
    16981 cgcagcggta tggaacgcgc ccttgaatac atgggcttga aagccggtac gccattaaac
    17041 gaaatccccg tcgacatcgt attcatcggc tcttgcacca acagccgcat cgaagacttg
    17101 cgcgaagccg ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca gcgcgtgtta
    17161 atcgtccccg gctccggttt ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt
    17221 ttcattgaag ccggttttga atggcgcgaa ccgggctgtt caatgtgtct cgccatgaac
    17281 gccgaccgcc tcgcaccggg gcaacgctgc gcctccacct ccaaccgcaa ttttgaaggc
    17341 cgtcaaggca acggcggacg tacccacctc gtcagccccg ctatggcagc cgccgccgcc
    17401 gttaccggcc gctttaccga catccgcatg atggcgtaag tattttcagg cggtctttaa
    17461 gaatttgacg cggccgtctg aaatatatcc cgtaaggcag aaacaccttt aatattcaaa
    17521 ccaaggagtt taaccatgaa caaacttttc attaccgccc tgtccgccct cgccttgtcc
    17581 gcctgcgccg gcacttggga gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca
    17641 caggccgccg ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggctgggac
    17701 aaaaccaaag aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc
    17761 ggcggaaaaa tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg aaaccgcttc
    17821 agacggcatc gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa ttaccgccat
    17881 cgtcgccccg ctcgaccgca gcaacgtcga taccgacgcc atcatcccca aacaattttt
    17941 aaaatccatc aaacgcagcg gcttcggccc caatgccttt gacgaatggc gttacctcga
    18001 ccacggcgaa ccaggcatgg acaacagcaa acgcccgttg aaccctgatt tctcgctgaa
    18061 ccagccgcgt taccaaggcg cgcaaatcct gttgacgcgt aaaaacttcg gttgcggctc
    18121 ttcacgcgaa cacgcccctt gggcattgga cgactacggc ttccgcgccg tcatcgcccc
    18181 cagcttcgcc gacatcttct ttaacaactg ctacaaaaac ggccttttac ctatcgtgtt
    18241 gaccgaagaa cgggttgacc gacttttcaa agaagttgaa gccaacgaag gctatcagct
    18301 ctccatcgac cttgccgaac aaaccctgac cacaccaagc ggcgaaacgt tcacatttga
    18361 cattaccgaa caccgcaaac actgcctctt aaacggcttg gacgaaatcg gtctgaccct
    18421 gcaacacgcc gacgaaattc acgcctttga agaaaaacgc cgccaaagcc agccttggct
    18481 gtttaacggt taaaacctat accgtctgaa ggccgttcag acggcattcc tgataacata
    18541 taattgttgc ggctaatgta aacagagggg cgataaattg ctttcatctt ttaagcgggc
    18601 ttagcgacac tctaaattaa tgcaataatg caactctcca tttcaaataa atttttagat
    18661 ttttaatatg caacaaatta aatttattga cttattttcc gaaatgagtg gcatcaggaa
    18721 aggattcgaa caagcctgtc ggaaacagtc ggttgcttgc gaatgtgttt ttacttcaga
    18781 aatcaaaccg gcagctttgg aagtattgaa gcaaaactac cctgatgaag tgccgtatgg
    18841 agatataacg aaaattgaaa caggggatat tcccgatttt gacatcctgt tggcaggctt
    18901 cccttgtcag gctttttctt tcgcctgaaa aagattaggc tttgaagata cacggggaac
    18961 gcttttcttt gatgtggcaa ggattttaaa ggcaaaaaaa ccaaaaggtt ttattttgga
    19021 aaatgtggaa ggattggtga cacacgatag aaaagattcg acacaaaaaa tagggcgcac
    19081 cctgaccgtt attttggaaa ccttggaagc attgggctac tatgtttctt ggaaagtttt
    19141 aaatgcaaaa gatttcggca ttccccaaaa caggaagcgc atttatctga caggcagttt
    19201 gaaatccaaa cccgacttat ctttcgaaac aagcccaagt ccgaaattaa aaaatatttt
    19261 ggaatcggga ctgcctaccg aaagcagccc tttcatcaaa aaattgctaa aaaaatttcc
    19321 cccgtccgaa ctgtacggaa aatcagtaaa agacaaacgg ggagggaaaa acaatattca
    19381 cagttgggat attgaattaa aaggcgcagt aaccgaagaa gagaagcaat tgttaaatat
    19441 ccttctaaaa gaacggagga aaaaaaatgg gcttcagaaa tcggcataga ttggatggat
    19501 gggatgcctt tgacaaaagc gcaaatttca actttctata aacaccccga tctccaaaat
    19561 attttggaca gcctgacaga taaaggctat ttggttttag agcatccgaa acaaaaaatt
    19621 ggcgggcaaa gaatcaaaga tgaatccctg cccaaaggct acaatattgt ttcaggcaaa
    19681 aaatcttttg aaatcaataa aatattagat caaaacgatg ttgcgccaac cttggttgca
    19741 atgaatatgg agcacctttt cgtcgttgac aacggcggtt tgcgtacact taccggaaaa
    19801 gaagggttgc gcttattcgg ctatccggac gattattcgt ttgatattcc caaaaaagac
    19861 agatgcgatt tattgggtaa taccgttgcc gtccctgtga ttaaggcggt atctgaaaga
    19921 cttctgcata ctttataaac cgacagggat aaaatgataa aacttactgc acaacaaata
    19981 tttgataagt tgctggatga agaaaaaatc ttatcggcca atggtcaaat tagatttttc
    20041 ttaggcgatg tggatattat cgtcaaacaa aaagatgttg tcggcaacat cattcaggaa
    20101 tggctcggcg gatgattgag aaaaagggaa attgaatttg atgtttcaac caatacccaa
    20161 atgcctcccg atttcttttt aaataaaaaa gaccgcagca gagaattgtt ggaagtaaag
    20221 gcgttcaacc gaaatgccgg cccgggtttt gatattgcag attttaaaat gtattctgat
    20281 gaaatcattc ataagcccta tatgctggat gtagactatt taatattcgg ttacgatatg
    20341 gacgacaacg gcaatgtaac catcaaggat ttatggctta aaaaagtatg gcaaattacg
    20401 agaagcatgg atgggtgggc aattaatctt caagtcaaaa aaggcgtggt gcataaaatc
    20461 cgcccgggtg tttggtacag cataaataaa aagaatatgc ctatgtttga atgcttggaa
    20521 gattttgttt ccgcaattga agaaaccgtt tatcaaaacc cggcaacacg gcataacgca
    20581 tccttatgga aaaagaaatt tgaggaagcc tataaaaaac actacaaccg atcgatttcc
    20641 atacctcgtt ggcacgaaat tgcacacaaa tacaaaaaga aataaccctt caaccatcaa
    20701 cccataagga aacttgaaat gaccaaacat atcgccatcc tccgaggtga cggcatcggc
    20761 ccagaaatcg tcgccgaaac cgtccgcgta ctcgacaaac tcatcgccca aggcttggac
    20821 gccagctacg aatacgcccc attaggcggc gaagcctacg acgaatacgg ccatccctat
    20881 ccccaattca cgcaaaacct ttgccgtaaa gccgatgccg tcctgcttgg tgcagtcggt
    20941 tctcctcaat acgacaatct cgaccgtcca ttgcgtcctg agcgcggctt gttggccata
    21001 cgtaaagact tgaatctgtt tgccaatttg cgccctgcta tcctctatcc cgagctagcc
    21061 aacgcctcca cgctcaagcc tgaaatcgtc gccggtttgg atattttgat tgtgcgcgaa
    21121 ctcaccggcg atatttattt cggcgaaccg cgcggcatcc gtgttttaga aaacggcgaa
    21181 cgcgaaggct acaacaccat gaaatacagc gaaagcgaaa tccgccgtat tgcccacgtt
    21241 gccttccaat ccgcccaaaa acgcagcaaa aaagtctgct ccgtcggcaa agccaacgtt
    21301 ttggaaacca ccgaattgtg gcgcgaaatc tttgaggaaa tcggcaaaga ataccccgat
    21361 gtcgagcttt cccatatgta cgttgataac gcagccatgc agctcgtgcg cgcgcccaaa
    21421 caatttgacg tgattgccac cggcaacatc ttcggcgata ttttatcgga cgaagcctct
    21481 atgctgaccg gctccatcgg tatgctgcct tccgcttctt tggacgaaaa cggcaaaggc
    21541 ctgtacgaac cgtcccacgg ctccgccccc gacatcgccg gacaaaacaa agccaacccg
    21601 cttgccacca tcctctcgct cgcaatgttg ctgcgttaca gcctgaacga cgaagcgcgc
    21661 gcgcaacaag ttgaaaacgc cgtacaaaaa gtgctgcaac aaggcttgcg caccggcgat
    21721 atttacgaag aaggcacgaa actcgtttcc tgctccgaaa tgggtgatgc ggtactcgcc
    21781 gccttgtaaa aaaacaaagc gcggatcaca ccgcgctttt tcatccgccc ttttccgccc
    21841 gccccgttcg tcaaccgcct gcaaacccac cgtttaccgg caggaaatgc cataaatccc
    21901 tgcctatgtt aggataaacg cctttaccgg cccacacaaa ggattatcat catgaaaaaa
    21961 atcatcatcg ccgcgctcgc agccgccgcc atcggcacta cctccgccgc cacctacaaa
    22021 gtggacgaat atcacgccaa cgcccgtttc gccatcgacc atttcaatac cagcaccaac
    22081 gtcggcggtt tttacggtct gaccggttcc gttgagttcg accaagcaaa acgcgacggt
    22141 aaaatcgaca tcaccattcc cgttgccaac ctgcaaagcg gttcgcaaca ctttaccgac
    22201 cacctgaaat ccgccgacat cttcgatgcc gcccaatatc ctaacatccg cttcgtttcc
    22261 accaaattca acttcaacgg caaaaaactg gtttccgttg acggcaacct gaccatgcac
    22321 ggcaaaaccg cccccgtcaa actcaaagcc gaaaaattca actgctacca aagcccgatg
    22381 ttgaaaaccg aagtttgcgg cggcgacttc agcaccacca tcgaccgcac ccaatggggc
    22441 gtggactacc tcgttaacgt tggtatgacc aaaagcgtcc gcatcgacat ccaaatcgag
    22501 gcagccaaac aataaatctt gatgcgaatc agcacaaatg ccgtctgaac gtccttcaaa
    22561 cggcatttgt gccgtcttca tcaaaccgcc ccgccaacgg taagccgata tggcggagca
    22621 caatccgccg aaagatattt cagcggcagg caggacttgc cggaaagtcc ggaaa
//
LOCUS       DAWIVB010000013        21295 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWIVB010000013 DAWIVB010000000
VERSION     DAWIVB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.013"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(59..1945)
                     /gene="uvrC"
                     /locus_tag="WIX60_000352"
     CDS             complement(59..1945)
                     /gene="uvrC"
                     /locus_tag="WIX60_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ5304675.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPNLPGVYRFFNES
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVREAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVRLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(2009..4171)
                     /locus_tag="WIX60_000353"
     CDS             complement(2009..4171)
                     /locus_tag="WIX60_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ5304676.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEF
                     AELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4310..5260
                     /gene="trxB"
                     /locus_tag="WIX60_000354"
     CDS             4310..5260
                     /gene="trxB"
                     /locus_tag="WIX60_000354"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ5304677.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5416..5784
                     /gene="rpsF"
                     /locus_tag="WIX60_000355"
     CDS             5416..5784
                     /gene="rpsF"
                     /locus_tag="WIX60_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ5304678.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5785..6087
                     /gene="priB"
                     /locus_tag="WIX60_000356"
     CDS             5785..6087
                     /gene="priB"
                     /locus_tag="WIX60_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ5304679.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6094..6324
                     /locus_tag="WIX60_000357"
     CDS             6094..6324
                     /locus_tag="WIX60_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ5304680.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6341..6793
                     /locus_tag="WIX60_000358"
     CDS             6341..6793
                     /locus_tag="WIX60_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ5304681.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(6986..7792)
                     /locus_tag="WIX60_000359"
     CDS             complement(6986..7792)
                     /locus_tag="WIX60_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ5304682.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(7793..8539)
                     /gene="pssA"
                     /locus_tag="WIX60_000360"
     CDS             complement(7793..8539)
                     /gene="pssA"
                     /locus_tag="WIX60_000360"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ5304683.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(8613..9017)
                     /locus_tag="WIX60_000361"
     CDS             complement(8613..9017)
                     /locus_tag="WIX60_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304684.1"
                     /translation="MANRFQQRKSAYRRLSGKPLFSDGIAANLCGGRKTLSYNLSRFK
                     ISIRSEMQKISFNLLKPANSPKIGKRRPGLNGAPHIPMPSPRYLPASPEMPGNNIHPA
                     KIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            9123..9605
                     /locus_tag="WIX60_000362"
     CDS             9123..9605
                     /locus_tag="WIX60_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ5304685.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFSELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGALR
                     VDNAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(9657..10868)
                     /locus_tag="WIX60_000363"
     CDS             complement(9657..10868)
                     /locus_tag="WIX60_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ5304686.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11091..13529
                     /locus_tag="WIX60_000364"
     CDS             11091..13529
                     /locus_tag="WIX60_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ5304687.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSSTD
                     LNGSRKILAHKDHL"
     gene            13737..15047
                     /locus_tag="WIX60_000365"
     CDS             13737..15047
                     /locus_tag="WIX60_000365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ5304688.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15143..15757
                     /gene="clpP"
                     /locus_tag="WIX60_000366"
     CDS             15143..15757
                     /gene="clpP"
                     /locus_tag="WIX60_000366"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ5304689.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCDRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLRL"
     gene            15781..15975
                     /locus_tag="WIX60_000367"
     CDS             15781..15975
                     /locus_tag="WIX60_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304690.1"
                     /translation="MPSETFRRHLFQAAFDMVSIPSSTGRKKDLEFFKGSPFPPVMDG
                     CRLKAKWGSSFCKGATLASS"
     gene            complement(16200..17465)
                     /gene="ispG"
                     /locus_tag="WIX60_000368"
                     /gene_synonym="gcpE"
     CDS             complement(16200..17465)
                     /gene="ispG"
                     /locus_tag="WIX60_000368"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ5304691.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGLKRHQGKVIPIHSL"
     gene            complement(17481..18242)
                     /gene="pilW"
                     /locus_tag="WIX60_000369"
     CDS             complement(17481..18242)
                     /gene="pilW"
                     /locus_tag="WIX60_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ5304692.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18245..19339)
                     /gene="rlmN"
                     /locus_tag="WIX60_000370"
     CDS             complement(18245..19339)
                     /gene="rlmN"
                     /locus_tag="WIX60_000370"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ5304693.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19482..19907)
                     /gene="ndk"
                     /locus_tag="WIX60_000371"
     CDS             complement(19482..19907)
                     /gene="ndk"
                     /locus_tag="WIX60_000371"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ5304694.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(19966..21117)
                     /locus_tag="WIX60_000372"
     CDS             complement(19966..21117)
                     /locus_tag="WIX60_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ5304695.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5101 a         5302 c         5788 g         5104 t
ORIGIN      
        1 ggtataccga cctgctgtat catatttttt acagacggat tttgtctttg acagcatgct
       61 aatgcagatg attgtaaatc gtttccgcca atgccttgct gatgccttcc actttttcca
      121 agtcctcgcg gctggcggca atcacgccgc gcagaccgcc gaagcgggtg agcagggctt
      181 ggcggcgttt gctgcctacg ccggggatgt cgcttaagga ggaggtaacg cgggctttgt
      241 cgcgtttttt gcggtgaccg gtaatggcga aacggtgcga ttcatcgcgt acggtttgca
      301 atagatgcaa ggccgggctg ttgggcggca ggcggaagac ttcgccggta aaaggcagta
      361 tgagctcctc cataccggct ttgcgctccg ggcctttggc aatgccgacc aaagggatgt
      421 gcagcccgag ttcttcccat accgatacgg ctacgccgat ttgccctttg ccgccgtcaa
      481 tcaacacggc atccggccat ttgacggtct cgccgttggc ttcggcctcc tgcattttgc
      541 cgtaacggcg cgtcaacact tcgcgcatgg cggcgtagtc gtcgccgggt ttggcggtcg
      601 tgatgttgta gcggcggtat tgcgaaggct ggatgttttg ctcatcgtac acaacgcagg
      661 acgcaatagt ggcttcgcct tgtgtgtggc tgatgtcgaa acattcaagg cggttgaggc
      721 cgtctgaatc catgccgagg atttttgcca gttcatcaat gcggtgttgc tggctgcttt
      781 gttgcaggcg gcgttgtgca atcgccattt gcgcgttttg ttccgccatt ttcaaccgga
      841 ctttgcgttc gcctatggtc ttggtgacaa attgcatctg cttgccgtgt tcgccttcca
      901 aagcctcttt tagcgcatcg ggaacgggaa agttgctgat gatgatgtcg ggtttgcttt
      961 tgcccagata gtgttgggcg acgaaggctt cggcgtaatc ttgtccgttt ggctcgggat
     1021 cgtttttggt gtcggggaaa aagcttttgt cgccgacgtg ccgtccgccg cggatgctga
     1081 cccagtgtac gcaaaccagg ccgtctgaaa ccgccagtgc aagcaaatcg atatcgttgg
     1141 gattgttcgg atttttactg tcgataaact gattactttg catgatgccg agcgcttgga
     1201 tttgatcgcg gtaacgtgcg gcttcttcga attgtagatt agcggcggcg gtttgcattt
     1261 tgtgttgcag ggtacgcgtc aattcgtcag ttttgccatt aaggaaagtc gcggcttcac
     1321 gcacgctgtc acgataatct tcttcactga tgtggcctac acaaggcgcg gtgcagcgtt
     1381 tgatttggta aagcagacaa ggacggtcgc gatgctcgaa tacactgtct tcgcaggtac
     1441 gcagcataaa gactttttgc aacacttgaa tgctgtcgcg cacggcgttg ctgttgggat
     1501 atgggccgaa atattgatta ggctttttca gcgtgccgcg gtaatacgcc atttgcggat
     1561 attgatggcc gctgagcatc aaataaggat agcttttgtc atcgcggaaa agaatattgt
     1621 atttgggcga cagggctttg atgaagttgt tttcgagaat cagggcttcg gattcggagc
     1681 gggtgatggt ggtttcgatg tggtgaacct gtttcaccat caatgcgatg cgcggggaat
     1741 ggtcgttttt ctggaaatag ccggacacgc gccgcttgag gttgacggct ttgccgacgt
     1801 ataagacgtt gccgctttcg ttgaaaaaac ggtatacgcc tggcaggttg ggcaggtttt
     1861 tgaggaaaag tgggatgtcg aagtgttccg gaaaacgggt ttctttattc atatcaatta
     1921 cttgaaaaac ggtttcagac ggcatattac catatcgctg gagacagcct tatccgttcg
     1981 gacaaggctg cagggcggtt acatgctgct aatcgatttt tacccgtttc aggcgcaagg
     2041 cattgctcaa cacggaaacc gagcttgccg ccattgccgc gccagcgatg acgggattta
     2101 aaaagccaag cgcggcgaga ggaatgccca aaatattgta gaagaaggcg aaaaacaggt
     2161 tttgcttgat gtttttcaaa gtggcttgcg acaccagcag agcatcggcg agttggttga
     2221 ccgaatgctg catcagcgtg gcggatgcgg tatgttcggc aacgtccgct ccgcctttca
     2281 tggcgaagct gacgttagcg gcggcaagcg cgggcgcgtc gttgatgccg tcgccgacca
     2341 tcgccacggt tttgccggcg gctttgagtt tttgcacttc ggcagcttta tcgcgcggac
     2401 tcatgttgcc gaaggcgtgt gcgatgccca gttgtttggc gacgtattcg accgtgcctt
     2461 ggttgtcgcc gctcataata tagacatcga tattgtgttt tttcagacgg cctatggctt
     2521 cggcggtatc ggctttcaac gcgtcggcaa gtgcgaatgc gccgatgggt ttgttatcga
     2581 ctgagaccgc aacaatgctt gcaatatccc aaacgccgtc taaaaacttc ggcaaggcca
     2641 gttcggcaaa ttcggctttg cctgctttca ccaaacccac gccttccact tcggcggtaa
     2701 tgcctgcgcc gacaacggtt tgtgcgtttt gtgcggcggg aatgtccaaa ccgcgcgctt
     2761 gggcggcgga gacgatggca cgggcgagcg gatgggcggc gttttgttcg acggcggcgg
     2821 cgatgcggta caaagcgtct tcgtcaaagc cgctgtcggg aacgcaataa acggcggcaa
     2881 cctgcgggct gccttcggtc agcgtaccgg ttttgtccaa cacgacggca tcgacgtggg
     2941 cggcttcctc cattgctgcc gcgtctttaa accaaatacc gtgtttaacc gctttgccca
     3001 taccgaccat aatcgcggca ggggttgcca gacccagcgc gcacgggcag gcaatcacca
     3061 aaacggcgac ggcgtgcatc agcgcaaccg tccaatcgcc cttaatcagc caagtaacaa
     3121 taaaagtcaa caacgcaatg cccacgacgg caggcacgaa taccgcagcc gctttatcgg
     3181 ctacgcgcgc aatcggtgct ttactgcctt gtgcttcaga gagcgcgttc atcatgtcgc
     3241 cgagctgggt ttggctgccg agctgcgtgg cgcggtacac cacactgcct tcggtcatta
     3301 acgcgcccgc caacactttg ccgcccgcct ttttttcttc aggattggat tcgccggtaa
     3361 gatggctctc gtccgcccaa ccgctgccgc tttcaatgat gccgtctgcg gcaatgcgtt
     3421 cgccgtggtt ggcgcggata aggtcgccga tttgcacttg gtcgatggga agctgtttcc
     3481 attcgccgtt gcgttgcacg ttgacttggg ttggtgtaag tttgagcagc aagcccaagc
     3541 tgttgaggct ggattttttg gtacggtgtt ccaaaaattt acccagtgac acaaaaccga
     3601 tcaccatcac gcccacttca aaatacacat gcgccatacc gtacgccgcg tgcgggctga
     3661 aaaacagcat atagacggaa tacaggtaaa tcgagaccgt gccgatggta accagcacgt
     3721 ccatattcgc cagtccgccc ttaatgctcg cccacgcgct tttgtaaaac gggattgcca
     3781 gccaaagctg caccacgctt gccaatgcga actgccacaa cggcggaatc atccaatcgt
     3841 gtctgccgat catcatcccc gccatgccga taaggaacgg gacgttgatg gtgaacagca
     3901 gccacagccg ccagccgata tggtgttctg cttcgggttg cggcaatgta tcttccgttt
     3961 tttccttcgc gccgtaaccg gttttctcaa tgattttggc aatgtcggct actgaggttt
     4021 tgctgtcgtc aaacactacc tgcgcctctt cgctggcgaa gtttaccccc gccgattcga
     4081 caaaatcttt tttgttcaac actttttcaa tgcgcgaagc gcaggcctgg caggtcatgc
     4141 cttcgatttg gaaacggatt ttttgttgca taagcgtgat tcctgctttg atattcagac
     4201 ggcagttaag gctgcgggat gccgtctgag cgccggtatg gtcgaaattt ctcaaacgcg
     4261 ccgataattc gagtagaatc cgaaaacttg acacagcaaa ggaaaaacaa tgtcccaaca
     4321 ccgcaaactg attattttgg gttccggccc cgccggatac accgccgccg tctatgccgc
     4381 gcgcgccaat ttaaaccccg tcattattac aggtatcgcg caaggcgggc aactgatgac
     4441 caccaccgaa gtggacaact ggcctgccga tgccgacggc gtgcaagggc cggaattgat
     4501 ggcgcggttt ctcgcccacg ccgaacgttt cggaacggaa atcatttttg accaaatcaa
     4561 cgccgtcgat ctgcaaaaac gcccgtttac actcaaaggc gatatgggcg agtacacttg
     4621 cgatgccctg attgtcgcaa caggcgcgtc cgccaaatac ctcggtttgc cgagtgagga
     4681 agcgtttgca ggaaaaggcg tttccgcttg tgccacctgc gacggtttct tctataaaaa
     4741 ccaagatgtt gccgtagttg gcggcggcaa tacggcagtt gaagaggcac tctaccttgc
     4801 caatatcgcc aaaaccgtta ccctgatcca ccgccgcagc gagttccgtg ccgaaaaaat
     4861 catgatcgac aaactgatga aacgcgtgga agagggcaaa atcatcctca agctggaaag
     4921 caacctgcaa gaagtactgg gcgacgatcg gggcgtaaac ggcgcattat taaaaaacaa
     4981 cgacggttct gagcaacaaa ttgccgtcag cggcattttt atcgccatcg ggcacaagcc
     5041 gaataccgat attttcaaag gtcagttgga aatggacgaa gccggttacc tgaaaaccaa
     5101 aggcggcacg gcggacaatg tcggcgcaac caatatcgaa ggcgtatggg cggcgggcga
     5161 cgtaaaagac catacctacc gtcaggcgat taccagcgcg gcttccggct gtcaggcggc
     5221 tttggacgcg gaacgctggc tgggcagcca aaacatttga atgccgtctg aaaccgaaat
     5281 tttcggcgga atgcaaattt ttcgcaccaa atccttgatt ccgtcggcaa accccaatat
     5341 aatgccgctt ctttaaacct tgccttctgc tttcgcatgg cagaaggctg tttttaccat
     5401 ccataaggag ataacatgcg tcattacgag atcgtgttta tcgttcatcc cgatcaaagc
     5461 gagcaagtac ccgctatggt tgaacgttac aaaaccatga ttgccgaagc aaacggtaag
     5521 attcaccgtc tggaagactg gggtcgccgc cagctggctt acccgattaa caaaatccat
     5581 aaagcccatt acgttttgat gaacatcgaa accactcccg aagtggttga agagctggaa
     5641 accgcattcc gcttcaatga tgcagtattg cgtcatctga ccatcaaaac caaacacgcc
     5701 gttaccgaag catcccctat gttgggtggt gaaaaggcta agaacctgtt gagcggtgcg
     5761 tctgaagaag cggtcgccca ataattggga ttcaataatc ttgtttcgct tgccgcgtta
     5821 attgaaaagg ttttccctat tcgatatacg cctgccggaa tccctgtttt agatattatt
     5881 ttaaagcacg aatcgtggca ggaggaaaac gggcagcaat gccttgtcca attggaaatt
     5941 ccggcacgga ttttaggcag gcaggcggaa gagtggcagt atcggcaagg tgtatatgtt
     6001 cacgtcgaag gttttttagc tcaaaaaagc agacgttccc ttatgccgat gctcaggata
     6061 caaaatattc aagaatataa aggttaaacg acaatggctc gtcaatcatt caaacgtaga
     6121 aaattctgcc gtttcacggc tgaaaaaatc caagaagtcg attacaaaca agttgatttg
     6181 ctgaaagact tcatctccga aaacggtaaa atcattcctg cacgcatcac aggaacgaag
     6241 gcattctacc aacgccaatt ggctgttgcc gtaaaacgcg cacgcttcct ggctctgctg
     6301 ccttacaccg accaacacaa ataattttgg agattgaatc atgcaaatta ttctgttaga
     6361 aaaaatcggc ggtctgggca acttgggcga catcgtaacc gttaaaaacg gctacgcccg
     6421 caactttcta attcccgcag gtaaggcaaa acgtgcgacc gaagcgaata tgaaagagtt
     6481 tgaagcacgc cgcgcagaac tggaagccaa acaggctgaa attttggcag atgcccgagt
     6541 ccgtcaggaa aaattggacg gtcaaaccgt taccgttgct caaaaagctg gtgtggacgg
     6601 tcgcctgttc ggttccgtta ccaatgccga cattgctgct gcaatcgttg ctgccggcat
     6661 cgaagccgtg aaagcaaatg tacgtctgcc gaacggtcct ctgaaagccg ttggcgagta
     6721 cgaagtggaa gtggctttgc acactgatgc cgttgctaaa attaccgttg ccgtcgttgc
     6781 cgcaaccgag taattgaaat tatcagatgc cgtctgaaac cctgccgatt gtcggaatgt
     6841 ggtttcagac ggtatttgtt ttgtttttcg tatattctgc aaaggtctcg gtcaggttgt
     6901 ctgtcctttt gtcggaacag gagactgggc agctttgtcc tgattaattg cttgtgcaaa
     6961 cggtaaaaag gttttttcgt gtgttttaaa gcaggttgta cagcattttt ccggcaatca
     7021 aaagcaacat aatgccgaag gattttttga gtttggcaga agaaagtttg tgggcggttt
     7081 tgacaccgag cggggcaaag gcaatggttg ccgcgctgag gacggcgacg gcgggcaggt
     7141 aaaggaagcc cagtgaccct tcgggcaatc ctgcaatatt caggccgttg agcagatacg
     7201 atattgcgcc ggagagtgca atcggccagg caaggccgga tgatgtgccg atggctttat
     7261 gggcggggaa gccgcagtgg attaagaagg ggacggaaag tgaaccgccg cctatgccga
     7321 cccagctcga cattgtgccg aacagtgtgg aaaccgcagt cagtccgggc agtccgggca
     7381 gcgggcggga tgccgtctga gggtcggtat gcagtgtttt gaatgcgacg gcggttaaaa
     7441 acaggatgaa gaaaatttga agcccgaacg cggggatata ttttgcggag agtgcgcccg
     7501 tgaatacgcc gaatatcata cccggcatca tcgtaaatac ggttttccag tcgaccgcct
     7561 gttttttgtg ctgccccagc atactggaaa aggcggtgaa gaccatgacg gcgaaggatg
     7621 tgccgacggc gaggtgttgc gcgtaaggat gttgtgccaa accctgcaaa tcaagcaccc
     7681 ataaaacgac agggacaatc agcgtgccgc cgcctacgcc gaacaggccg gcaataaaac
     7741 ctgccgcact gcctacggca agcaggatta agataatgtc ccaatgccac atttaatccg
     7801 cctttctgta ctttttccaa aatcggcgtg ccgccataat gtagccggac aggctgtatc
     7861 cgagaaagaa caggaagagg acgagcgacg gttcccaagt gaccagaagc agcagtaaga
     7921 cggcaagcag cattccgaca aaggggactt gtctgcggat gttgatttct ttaaaactcc
     7981 aaaaagggat ttggacaatc atcgacaggc cggcaaacag tgtgatgccc aatgcccacc
     8041 agtggacggc ggggaatttt tcgacgctgt ggttgaccca aatcagcccg acaatcagcg
     8101 cggcggcagt cggactgggc acgccgataa accagcgttt gtccaccttg ccgatgagtg
     8161 tgttgaacag ggcgaggcgc agggcggcgc aggcgcagta gatgaaggcg acggaataac
     8221 cgattttgcc gaactgccaa agctgccatt tgtaggcaat cagagcggga gcaacgccga
     8281 agctgaccat atcggcaagg ctgtcgagct gctccccgaa cgcgctttgg ctgttggtca
     8341 gccgcgccac gcgcccgtcc ataccgtcca gcaacataga gatgaatacc gcgatggcgg
     8401 cggtttcata acgtccgtgc atggattggg tgattgcgta aaacgcggaa aacagcgcgg
     8461 cgatagtaaa ggaattgggc agcaggtaga tactgttttg acgcagggag cgttgcgggg
     8521 tgttggtcgg ggtttccata gctgaagtgt ctcacggacg tggagaatcg ataggggatt
     8581 ataacccaaa ccattatcga acaatacgcc ctttaggatg aagccctcgg aggcggtttg
     8641 tttttctgat ggcggatgct ttgcggggcg gtttgactct gcctgccgcc gggtgtgttg
     8701 tttcggattt ttgccggatg gatgttgttg ccgggcattt ctgggcttgc cggaaggtat
     8761 cggggggagg gcatcggaat atgcggcgcg ccgttcaaac cggggcggcg tttccctatt
     8821 ttcggggagt ttgcaggctt caacaaattg aaagatattt tttgcatttc cgaccgtatg
     8881 ctgattttga aacgggataa attataggac aaggtttttc gcccgccgca aaggttggcc
     8941 gcaatgccgt ctgaaaacaa cggcttgccg ctaaggcggc ggtatgcgga tttgcgctgc
     9001 tgaaatcggt ttgccatacc cggcggtgca atctgcgggg agggataagc cgataaggcg
     9061 ggacttatcc tgttttttgt ttataatgca acagaatttg gacgaaccgt cgggaattta
     9121 tcatgatgaa aaagatactg gccgtatcgg cactatgcct gatgactgcg gcggcacggg
     9181 ctgccgatac ttacggctat ctcgccgttt ggcagaatcc gcagaatgcg gacgatgttt
     9241 tgcaggttaa aaccacaaaa gaagattcga cgaaaagcga agcgttttcc gagttggaag
     9301 ccttctgcaa aggtcaggac acgcttgcgg gcattgctga agacgagccg accggatgcc
     9361 ggtcggtcgt gtcgctgaac aatacctgtg tcgcgctggc atacccgaaa gccttgggcg
     9421 cgctgcgtgt cgacaacgcc gtcgtgatta cttctccgcg ttttacgagc gttcatcagg
     9481 tcgcactcaa ccagtgcatc aaaaaatacg gcgtacaggg acaatgcggc ttggaaacag
     9541 tgtattgcac atcttcttct tattacggcg gaactgtgcg ctctttgatt caaaatctca
     9601 aataaaacgg aaaatgccgt ctgaaagatg ttcagacggc atttctatat cgacggtcag
     9661 gattctttcg gatcgggcag caggctgttc aacataatgg caagtacggc gcacaagccc
     9721 acgccggcaa agctgaagct gcccaatttg agcgtcatgc cgccgatgcc cgtggtcagt
     9781 accgagctga cgatgaccag gtttttcggc agcatcaaat cgactttggc atcaatcagc
     9841 gttttcacgc ccaaagaagc aatcgtgccg aacagcagca gcataatgcc gcccattact
     9901 ggcatcggaa tggaagccaa aaacgcattg aatttgccga aaaacgccat gcagacggca
     9961 aaaaccgccg cccaagtcat gatgacgggg ttgctgtttt tggtaatcat caccgcaccc
    10021 gttacttcgc cgtaggtcgt aaccggcggg ccgccgatca gacccgcaac gcatacgccc
    10081 aaaccgtcgc ctgcaagggt tttgtccaag cccgggtctt tcgtatagtc tttccccgtc
    10141 acattgccga ttgccatgat gccgccgatg tgttcgatgg cgggggcgac ggcaacgggc
    10201 agcataaaca gtgcagcctg ccagttgatc tgaggcgttt caaaatgggg aacggcgaac
    10261 cagggcgcgt gtgcaatgct tgccgtgtcc accagtccca tcagcagtgc caaaacataa
    10321 cccgaagcga caccgatcaa gatgggaatc agcttcatca tcctgctgcc gaaaaccgat
    10381 acgatggcgg taacggcaaa ggtaaagccg gaaagaatca gcgaatcggt atagtcgatg
    10441 acctgtttgc cgtccgcctg acccattgcc atgctgcttg ccgccacggc aacagacaga
    10501 ccgatgacca tgatgacggg gccgatgacg accggaggca gcagtttgtg taccgctgcc
    10561 agtccgcgcc aacggatcag cgcggcaaac acaaaataca taaagccggc ggcaaacagt
    10621 ccgaacatgg tggaaggcag cccccattcg ccgacggagt agataatcgg tgcgataaag
    10681 gcaaacgaag aaccaagaaa aatcggcact ttgcgtttgg ttgtgatttg gaacagcagc
    10741 gttcccaagc ctgcgcccaa aagcgcaaga gccggattca gaccggtcag caggggaacc
    10801 agcaccattg cgccgaatgc cacaaataaa atctgtgcac cggaaacggc aagtttcagt
    10861 tggttcatac catcttcttt caatcggttc aaacggcacg gattatactg gtttgccgag
    10921 ggtctgcgta aaaatacata cgtcgggcaa cagatatgcc gtctgaaagg caatgtctgt
    10981 ctgttgcagg atgaaagcag gagaaatgga gtataatccg acactttcca tattcaatcc
    11041 gcaagccgtc cggcttcctt atttcagacg gcattttccg gtaatacggc atgacagaaa
    11101 aatcccataa aaaaacagca aaaggcaggg cgggaagccc gtccccaacg tccgcacgca
    11161 acaagaaagc cgacaacggc gcacggggca ataaagtttc cgagcggctc aaggcggtca
    11221 aagagttgca gaaaaccgaa accaaaaagg cgcgtcccga acatgtcgtc aaccttatcg
    11281 gcgacgcact gtggctgatg ggtttggcgg caaccctgta tttggcgatt tccctgatca
    11341 gtttcgatat gggcgatccg tcttggtcgc acagttcgcc ggttgtggaa gatgtcgcca
    11401 attggggcgg actgttcggc gcgtatgttg ccgatgtcgg ctattatctt ttcggctggt
    11461 cgttctggtg gtggatagcg gctgcctgcg tcgtgctgta taaaaatttc cgcctgcacg
    11521 caaaacagac ggaaaacgag gcatacaacc acaaaatcgc tgccgccgcg ctgtttgtcc
    11581 tgacggtctt cagccccgtc ttggagtatt ttgtgctggg cggaaaatat gccgactccc
    11641 tgcctgtcgg agcaggcggt atggtcggca tacgcgtcgg cgcagtgttt gcgtggctgc
    11701 tggggaaatc gggcagcctg ctgattatct tggttgttct gctgttgtcg ttgtccctgc
    11761 tggtgcagat ttcatggctg gaatttttga acggtgcggg cagggcggtt caaaaccgcc
    11821 tgagtgcctt atccggcaag gtcatggctt taggaaaacg ccggccgaat accaaaacag
    11881 acggtgtcga tacccaaaat acacggcgca tggtaaaaga agccaagaat attacggcca
    11941 aacccgttgc cttgcccgaa ggcagcagca gcaaccgcaa atccgtcgcg gtttccgtcg
    12001 cgccgccgcc caaaattcag gtttctctgt ttgaagatga cgaacctcgg caggcgggcg
    12061 aataccacaa gcctacattg aacctattgc ggattcctga cagcgaaccc gtcagcatca
    12121 atcccgccga attggagcgc actgccgaac tgatcgaatc caaactggca gaattcggca
    12181 tcggcgtaca agtcgtatcc gccacatccg gccccgtcat cacgcgctac gaaatcgaac
    12241 ccgcgcaagg tgttaaaggc agccaaattg ttgccttgtc gaaagatttg gcacgctcta
    12301 tgtcgctgca gtccgtgcgt atcgtcgaaa ccatcgcagg taaaaacacg atgggcatcg
    12361 agttgcccaa cgacaaacgc caagacgtga tgttgagtga aatcttgtcc tcgcccgtgt
    12421 ttgccgaagc caaatccaag ctgaccgtcg cgctgggcaa agacattgcc ggtacccccg
    12481 ttgtcggcga cttggcgaaa atgccgcacc ttttggtcgc cggtatgact ggttcgggca
    12541 agtccgtcgg cgtgaacggc atgattatgt ctatgctttt caaagctacg cccgacgaag
    12601 tccgcttcat tatgatagac ccgaaaatgc tcgagttgag catttacgac ggtattccgc
    12661 acctgctctg tcccgtcgtg accgatatgc gcgaagcagg gcaggcgttg aactggtgcg
    12721 tcgccgaaat ggaaaaacgc taccgcctgc tttcccatgc cggtgtgcgt aatttggagg
    12781 gcttcaacca aaaagtcgaa gccgcaaaag cggcaggcaa gccgctgctc aatccgttca
    12841 gcctgaaccc cgacgagccc gagccgctgg aaaaactgcc gttgattgtg gtcgttatcg
    12901 acgaacttgc cgacctgatg atgaccgaac gcaaagccgt cgagcagcaa atcgcccgtc
    12961 tcgcccaaaa agcgcgcgcc gccggtatcc atatgattgt cgccacccaa cgtcccagtg
    13021 tcgatgtcgt taccggcctg attaaagcca acatcccgac gcgtatggcg tttaccgtgc
    13081 aaagcaaaat cgacagccgt accatcctcg accaaatggg cgcggacgaa ctgctcaaat
    13141 atggcgattc gctgttcctc cagcccggca gtgccgaacc gactcgcctg caaggcgcgt
    13201 ttgtttcaga cgacgaagta catcaagtcg tcaactatgt caaatcgcaa gccccagccg
    13261 actatattga aggtctgctc agcggcgagg ccgcgctgga aactgccaat atcgttaatc
    13321 cgaatgcaga cagcgacgaa ttgttcgatc aggcagtcgc ctatgttttg gaaagcaaaa
    13381 aaacctccat ttcgtctttg cagcggcagc tgcgcatcgg ctataaccgc gcggcaaacc
    13441 tgatggaggc actggaaaat gcgggtgtcg tttcttccac cgacctcaac ggcagccgta
    13501 aaattttggc gcacaaggac catttgtagc ccgtattgca aaatgccgtc tgaacggcgg
    13561 aattggcgtt tcagacggca tattatgttt caggcgaaac attgtgatat acttgccagc
    13621 taaaatttcc cctttgcggc aatgcggttc aaatatcgta ccgttgcgct gtttgcttcc
    13681 ccatgtaggg aagaaagttt atcattttat caacacaaca aatttaaggg cttatgatga
    13741 gcgtaactgt tgaaacttta gaaaatctgg aacgcaaagt agtgttgtcc ctgccttggt
    13801 ccgaaatcaa cgcagaaacc gataaaaaac tgaaacaaac ccaacgccgt gcaaaaatcg
    13861 acggtttccg tccgggtaaa gcacctttaa aaatgattgc ccaaatgtac ggtgcgagcg
    13921 cacaaaacga cgtgatcaac gagctggtgc aacgccgctt ctacgatgtt gccgttgccc
    13981 aagagttgaa agtggcaggc ttcccccgtt ttgaaggcgt tgaagaacaa gacgataaag
    14041 agtctttcaa agttgccgcc atttttgaag tgttccccga agtcgttatc ggcgatttgt
    14101 ctgcacaaga agttgaaaaa gtaaccgctt ccgtcggtga tgccgaagtg gaccaaaccg
    14161 tagaaatcct gcgcaaacaa cgcacccgct tcaaccatgt cgaacgcgaa gcccgaaacg
    14221 gcgaccgcgt catcattgac tttgaaggca aaatcgacgg cgaacctttt gccggcggcg
    14281 catccaaaaa ctacgccttc gtattgggcg caagtcaaat gctgcctgaa tttgaagccg
    14341 gcgtagtcgg catgaaggct ggcgaaagta aagacgttac cgtcaatttc cctgaagact
    14401 accacggtaa agacgttgcc ggtaaaactg ccgtgtttac cattacgctg aacaacgttt
    14461 ccgaagcgac tctgcctgaa gtcgatgcag attttgcaaa agccttgggt attgcggatg
    14521 gcgacgttgc caaaatgcgc gaagaagtgc agaaaaacgt aagccgcgaa gtggaacgcc
    14581 gcgtaaacga acaaaccaaa gaatccgtaa tgaacgcgct gctcaaagcc gtagagctga
    14641 aagcacctgt tgctttggtc aatgaagaag ccgcacgctt ggcaaacgaa atgaaacaaa
    14701 attttgttaa ccaaggtatg gctgatgctg ccaacttgga tctgcctttg gatatgttca
    14761 aagaacaagc cgaacgccgc gtatctttag gtctgatttt agccaaactg gttgacgaaa
    14821 acaaactgga accgactgaa gagcaaatca aagccgttgt tgccaacttt gcagaaagct
    14881 acgaagatcc tcaagaagtg attgactggt actacgcaga tccttcccgc ctgcaagccc
    14941 cgacttcttt ggcggtagaa agcaacgtcg ttgatttcgt tttgggcaaa gccaaagtaa
    15001 atgaaaaagc tttgtctttt gacgaagtga tgggcgcgca agcctgatta tcctgaaaat
    15061 gccgtctgaa ggcagttttt gaaagcaccg aagccgcttt taaagctcgc tttggtgctt
    15121 ttccatcatg aaaggagacg aaatgtcttt tgataactat cttgtcccta ccgttatcga
    15181 gcagagcggt cgcggtgagc gtgcattcga tatctattcc cggcttttga aagagcgcat
    15241 cgtattcttg gtcggaccgg taaccgacga gtccgccaat ctggtggttg cccaactgtt
    15301 gtttttggaa agtgagaatc cggataagga tattttcttc tatattaact cgccgggcgg
    15361 ttcggtaacg gccggtatgt cgatttacga caccatgaat ttcatcaagc ccgatgtatc
    15421 gactttgtgc ttggggcagg cggcaagtat gggcgcgttc ttattgtcgg caggcgagaa
    15481 aggcaaacgt tttgccctac ccaacagccg gattatgatt caccagcctt taatcagcgg
    15541 cggtctgggc ggtcaggcat ccgacattga aattcacgca cgcgaacttt taaaaatcaa
    15601 agaaaaactc aaccgcctga tggcgaaaca ttgcgaccgc gatttggcag atttggaacg
    15661 cgacaccgac cgtgataatt tcatgtctgc cgaagaagcc aaagaatatg gtttgattga
    15721 ccagattttg gaaaaccgcg cttctttgcg gctttaataa aagaaacctg agagaaaccg
    15781 atgccgtctg aaacgttcag acggcatttg tttcaagctg cgtttgatat ggtatcgata
    15841 ccgtcatcca cgggacggaa gaaggatttg gagtttttta aaggatcccc gttcccgcct
    15901 gtgatggacg gatgccgtct gaaagccaaa tggggcagca gtttttgcaa gggcgcgaca
    15961 ctcgcatcct cctgaatctg ccggtacggt tattgagaaa agacaagggc aggaagcggt
    16021 atcccgcctt ccttatttat acctgcaatt catctccgta tttgccgatt gtattttcat
    16081 attgcgcctc cattccttat cggtttaaaa aaatgccttt tccaatggga tgggttttat
    16141 atcaatgaaa ataaacggtt tttatttaaa aatagccaag gtaggctggc ggttggattt
    16201 tatagggagt gtatcgggat aaccttccct tgatggcgtt tgagtccgtt ctccccataa
    16261 ttggttttga catactcttc aacaatagcc agaaattccg ttgcaatgtt gtcgcctttc
    16321 agcgttactt tacgttcacc atctacataa acaggggcaa cgggtgtttc tcccgtaccg
    16381 ggcaggctga tgccgatgtc ggccaatttg ctttctccgg gcccattgac aacgcagccc
    16441 attacggcaa cgttcaggga ttcaacccca ggataaaggg tacgccatat agacattttt
    16501 tggcgcaggt aattttgaac atcttgtgcc agctcttgaa atacggtact ggtggtacgc
    16561 ccgcagccgg ggcaggcggt aaccatcggc gtaaacgaac gcaatcccat agtctgtaaa
    16621 atctcttgcc cgacgacgac ctcctgagta cgcgggctgc caggttccgg agtcagtgaa
    16681 atgcggatgg tgtcgccgat tccttcttga agcaagacgg ataatgccgc cgttgatgcg
    16741 acaatgcctt tgctgcccat accggcttcg gtcaaaccca aatgcagcgg ataggcgcaa
    16801 cggctgccca gttcgcggta aacctgaatc aaatcctgaa ccgcgctgac tttgcacgac
    16861 aggatgattt tgtcttcggg cagtcccaat agaacggctt tttcggcaga ttccaaagcg
    16921 gagacaatca gtgcttcctt cgtcacttct tcgggcggtt tcggcgcgga agaagcgagg
    16981 ttggcatcca tcatacgttt ggcgaggctt tgatccaaag aaccccagtt tacgccgatg
    17041 cggacggctt tatcgttttc agcagcagtc cgaatcataa aggcaaattt ttcatcgcct
    17101 tttacgcctt tgccgacatt gccgggattg atgcggtatt tggacaatgc tttgccgcat
    17161 tctggaaatt ccgccaacag gcgttcgccg ttgaagtgga aatcgccgat aagcggtgtg
    17221 gcatagccca tatcgtccaa gcggcggcgg atttcggcaa ctttggacgc ggcttcgggg
    17281 ctgttgacgg taatacgcac catttcggat ccggcatcgc tcaattcctt aatctgcaat
    17341 gcggtggctt ttgcatcggc agtgtcggtg ttggtcatag attggataac gacgggtgct
    17401 tctgaaccga cggtaatatg atcgatgcgg acttgatgcg tcttgcggcg ttggagtgtg
    17461 ttcatatggt ttgaatctgt ttattgaccg gtgaggacgg tttgcaattc ttccgagtag
    17521 gggaaattcg cctgcaattg tgcttcatat tcgtatgccg cctgtgcgtt gccgagggct
    17581 ttggcaattt tccagcctag cagcaaatca tcggcctgaa ggacttctac cctgctttgg
    17641 tattttttaa agtagtaatc ggcatcgccc aactgcccgg ccagcatttt ggtgcgcgcc
    17701 agttctttaa atgcgggtgg gaactgcggc tgggcggcga gggaacgttt caaataggct
    17761 tccgccaatc cgaattgccc ctgttttgcg ctgcatatgc ctttattcag gttggcaata
    17821 taaggggtcg ggtaggtggg gtcggccaga gctttgtcga aatatgccat agattcggca
    17881 gggcggttga gcctgccgca taggaaccaa ccgtagttgt tgttgatttc ggcactgtcg
    17941 ggtttgatgg agagggcttg ccggaaactt tcctgcgcct tgtcgttaac tttcaggtat
    18001 tgatagattt cggcacggac cagccaggca agctcgtttt tagggtccga tttcagggcg
    18061 tcttcaatac ttgccgtcgc ctgacggtag tcctgaccgc gcatatattc cattgccaac
    18121 tgggttttga tattggaaac ctgattggct ttttctgccc gcgaggggcg gtaggaagtg
    18181 ctgcacgcgc ccaaggcaag aacgagtaat aaagagattc gtttggatgg cttaaaaggc
    18241 ataattaccc ctgttgtccg attaaaatct gctgccattt ttgttggcgg cgcgttttat
    18301 cctgaacctg ccccgccaac tgtccgcagg cggcatcgat gtcgtcgccg cgcgtttttc
    18361 gtacggtaac gacaaatcct gcctgctgca aaatatcgcg gaacacacgg atgttctcat
    18421 tgctggagcg ttcgtatccg gagtttggga agggattgaa cggaatcaga ttgaacttgc
    18481 agggaacatc tgtgaccagt tcgatcagtt cgcgcgcatg ttgcgcctta tcgtttattc
    18541 cgtccaacat gacgtattcg aaagtgatga aatccctggg tgctttgacc agatagcgtt
    18601 ggcatgcggc catcaattct ttcaagggat attttttgtt caacggtacg atttggttgc
    18661 ggacttcgtc attggaagcg tggagggaaa ccgccaaagc caccggcatg acatcgcgca
    18721 acctgtccat ttggggaacc atacccgaag tggaaacggt tacgcggcgg cggctcaaac
    18781 cgtagccgtg gtcgtccagc atgatgctta aggcggtaac gacattgtcg aagttcgcca
    18841 tcggctcgcc catgcccatc atgacgacgt tggaaatcac gcgctcgttt ttcggtgtaa
    18901 cgcccatcgc tttgtttgcc caccacaatt gcccgatgat ttcggcagca gtcaaattgc
    18961 ggttgaagcc ctgccggccg gtcgaacaaa atgtacattc caaagcgcag ccgacttgtg
    19021 aggaaatgca gagcgtgccg cgatccgatt cggggatgaa gacggtttcc acgccgttgc
    19081 ccgtaccgac atccaaaagc cattttcgag tgccgtctga agatttttga gacatcatca
    19141 gcttgggaat ttcgatgcct gcctgttcgt tcagtttatg gcgcaacgat tttgccaaat
    19201 cggtcatttc gtcaaaattt tgcgcgccgg attggtgcat ccaacgcata acctgtttgg
    19261 cacggaaagg tttttcgccc atatcggcaa aatgtcgggt cagcccttga aggtcgtagt
    19321 tgagcagatt ggttttcatg tgtttgtgtt tttcttaaat caaggcttca aataaaaaat
    19381 tgcagggcag gcaagtttga tttgaagcct tttgcaacag agttttcaga cggcatggat
    19441 atatttatgc cgtctgaaga ggagttgggc ggtgtattgt atcaacgggg gcagatttcg
    19501 gtttggctga aaaagtaggc aatttccaaa gcggcatttt ccacgctgtc ggaaccgtgt
    19561 acggcattaa tgctgaccga agtggcaaag tccgcgcgta tcgtgccttc ggcggcttcg
    19621 gaaggattag ttgcacccat cagttcgcgg tttttcagga cggcgttttc accctctaat
    19681 acctgaatca taaccggacc gccggtcata aattcaacca atccggcgta gaaggggcgg
    19741 tctttatgaa ccgcataaaa ttcttgcgcc tctttgagag taagctgctt cattttggcg
    19801 gcaacgattt tcagaccgtt ctcctcaaag cggctgtata ttttgccgat aacatttttg
    19861 ccgacggcat cgggtttgat gatggagatg gtacgttcaa tcgccatgct atatccttat
    19921 ttttacttaa gaagaatcaa atcggtattc tatcaaaaaa ataacttatc cgcctgaagc
    19981 cttgggagat agggtcaggt gcggctgttc caaataaact tcggactgca tttcgaccat
    20041 ccggctggcg gtgcgggtaa attcttcggc aaaatcgcct tccgtataga tatggttgac
    20101 atctaccgcg ccggtggcac acagtttgac ccggaaatcg tagagtacgt caatcagcca
    20161 agtcagccgc cgcgcctccg ccttttcttg cggtgagagt tgttccaaac ctgaaataaa
    20221 aaccatttca taatgttcgg ccaaatacag atagtcggac tgtgagcggg ggccgaagca
    20281 cagtgcgcgg aaatcaaacc atatggcacg gccggactcg gctttgtggg gaatctcccg
    20341 accgtggatg gtgctgatgc cggggttcaa atcggtaatg cctgtcattt ctttgaacag
    20401 ttttgccagt tttgcctcat tttcttcatt ggcaggcgta aagaaaatct cggcggggcg
    20461 gagggtacgc agtcggtagt cttcaccgcc gtcaacgttt aagacggtca ggctggactc
    20521 gatgagcgcg attgtgggaa gaaaactgct ccggttttga ccttgcgggt agagttcgga
    20581 aggcgcgtag tttgaagtcg ccaccaaaac aacgccctcg ttaagcaggt tttccagcag
    20641 acggcctaaa atcattgcat ccgcaatatc gctgacatga aattcgtcaa aacacaatac
    20701 gcgggtttct ttggcaatct cggcggcaac ggatttcaac gggttgcttt cgcttttcag
    20761 ggttttcagc cgctggtgga tttctgccat aaaggcatga aagtggacgc ggcgtttgcg
    20821 gcggtacggg aggcagccga aaaaagcgtc catcagaaag cttttgccgc gtccgacccc
    20881 gccatagaaa taaagccctt tggggacttg cggggaacgc aaactcctgc ctaaaaaacg
    20941 gtttcttttg cgtttgaaca tcatcaattc ggtccaaagc cgatcgaggt gttcgatggc
    21001 ggctgcctgc gcgtcgtcgc ggatgaagtt gggcagttgt gaggcagcct gataccaggt
    21061 cagcgggctg tggttttcaa acggcggggc tttaaaaagt tggtctctat tactcatatt
    21121 taaccttgtt atacttcttg cggctaagaa agaaaatgct taccgtcggt attataaatg
    21181 atttccttca aacttggcat tttccgtgga aaagaataat gacgcgcaca agggggtata
    21241 agcaaaggcg gcgtttcaat atggtgcaga tatgagctca aatgcctttt atagt
//
LOCUS       DAWIVB010000014        21154 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWIVB010000014 DAWIVB010000000
VERSION     DAWIVB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21154
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.014"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..144
                     /locus_tag="WIX60_000373"
     CDS             <1..144
                     /locus_tag="WIX60_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ5304696.1"
                     /translation="NVGIGGGALLGHWVTQYSGISCIGVAGMLTAAAGLWVCLNLNRH
                     IRT"
     gene            273..1709
                     /locus_tag="WIX60_000374"
     CDS             273..1709
                     /locus_tag="WIX60_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ5304697.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGIAASLTVLGFFKYFDFFRPMI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRRARSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRDALCRLKYFAPLSWLITFHFVCLSFVVFNTANPD
                     DAGAVFSALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1720..2703
                     /locus_tag="WIX60_000375"
     CDS             1720..2703
                     /locus_tag="WIX60_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ5304698.1"
                     /translation="MKNFLSLFSSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKLYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTTHTLSGGKDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2700..3893
                     /locus_tag="WIX60_000376"
     CDS             2700..3893
                     /locus_tag="WIX60_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ5304699.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNASASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDG
                     IASKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRS
                     AAIRQ"
     gene            complement(3953..4129)
                     /locus_tag="WIX60_000377"
     CDS             complement(3953..4129)
                     /locus_tag="WIX60_000377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304700.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAVCRRFACVLPMPEANAGCV
                     RQTVRGVAMPSEAI"
     gene            4180..5733
                     /locus_tag="WIX60_000378"
     CDS             4180..5733
                     /locus_tag="WIX60_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ5304701.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPTGETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5823..7850)
                     /locus_tag="WIX60_000379"
     CDS             complement(5823..7850)
                     /locus_tag="WIX60_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ5304702.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKQTASPAMHELQRELSEEYGLSVR
                     VDKMFHRDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8139..10145
                     /locus_tag="WIX60_000380"
     CDS             8139..10145
                     /locus_tag="WIX60_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ5304703.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGSLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            complement(10268..11377)
                     /gene="mltB"
                     /locus_tag="WIX60_000381"
     CDS             complement(10268..11377)
                     /gene="mltB"
                     /locus_tag="WIX60_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ5304704.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRAVEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(11286..11537)
                     /locus_tag="WIX60_000382"
     CDS             complement(11286..11537)
                     /locus_tag="WIX60_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304705.1"
                     /translation="MPSDGIFGDDIRISGGARGNVRHSAKPPIAADAAFRAAPLCRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPTPGK"
     gene            complement(11579..13006)
                     /locus_tag="WIX60_000383"
     CDS             complement(11579..13006)
                     /locus_tag="WIX60_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ5304706.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYIDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLIPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIKFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDTAAFLLNDIRKDILDCRYCG"
     gene            13191..17330
                     /gene="mfd"
                     /locus_tag="WIX60_000384"
     CDS             13191..17330
                     /gene="mfd"
                     /locus_tag="WIX60_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ5304707.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLDDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQYLYLSADVFAGRLKNYGQVLP
                     DVSGKEYTLPDLAVNRQADEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQPTPSPVGEGWGEGKAVA
                     AQTEFSAAAINPLPSPLPQEREQSAAAVSDSLKAAAVSTESSLPLGTSNLHGQIRQQP
                     APSPVGEGWGEGKAVAAQTEFPASATNPLPSPLPQEREQSAAAVSDDLKTKSSLHPVA
                     NNLHGQIRQQPTPSPVGEGWGEGKAVAAQTEFSAAATNPLPSPLPQEREQSAAVVSDS
                     LKAAAVSTESSLPPGKSNLHGQIQQQPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQ
                     YVARSRIHNRRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLVTMDLGGETNE
                     MMLLEYAGEAQLYVPVSQLHLISRYSGQAHENIALHKLGSGAWNKAKRKAAEKARDTA
                     AELLNLYAQRAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMD
                     RLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVA
                     SLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQK
                     EQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGS
                     VREAVLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMR
                     DFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAY
                     LLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFT
                     LYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPEDYCPDIHERLVLYKRLA
                     VCETVQQINTIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTF
                     GKNNNVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLP
                     K"
     gene            17479..17862
                     /locus_tag="WIX60_000385"
     CDS             17479..17862
                     /locus_tag="WIX60_000385"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ5304708.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            17884..18726
                     /gene="kdsA"
                     /locus_tag="WIX60_000386"
     CDS             17884..18726
                     /gene="kdsA"
                     /locus_tag="WIX60_000386"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ5304709.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            18741..19181
                     /locus_tag="WIX60_000387"
     CDS             18741..19181
                     /locus_tag="WIX60_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ5304710.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            19229..20515
                     /gene="eno"
                     /locus_tag="WIX60_000388"
     CDS             19229..20515
                     /gene="eno"
                     /locus_tag="WIX60_000388"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ5304711.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            20530..20808
                     /gene="ftsB"
                     /locus_tag="WIX60_000389"
     CDS             20530..20808
                     /gene="ftsB"
                     /locus_tag="WIX60_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ5304712.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(20849..21139)
                     /locus_tag="WIX60_000390"
     CDS             complement(20849..21139)
                     /locus_tag="WIX60_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ5304713.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCIEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         5054 a         6412 c         5272 g         4416 t
ORIGIN      
        1 aacgtcggta tcggcggcgg cgcgctgctg gggcattggg ttacgcaata ctcgggcatt
       61 tcctgcatcg gcgttgcggg tatgctgacg gcggcggcag gtttgtgggt ctgcctgaac
      121 ctgaaccgcc atatccgaac ctgaccgcac gatgccgtct gaagcccctc ccgcccttca
      181 gacggcattt tgattgaaga aatatccacc cctgcttaaa ataacgggct ttgccgtgtt
      241 ttaacgccta ttttttgttc caaggatggt ttatgccgct gctgtctgtc gagttcgcac
      301 tgttctttct cgccttcctg ccgatttact ggggcttggc gaaatacccg tccgtccaaa
      361 acctgctgct tttggctgcc ggtatgggct ggctctacca tatcggccct gtatttgcgg
      421 caatcatcgt cctttattcc tcctgcgtgt accttttggg cgaactgctc cgttccgatc
      481 gcgaaaatac gcgccgtttc tggctggggt gcggcattgc cgcctcgctg accgtcttgg
      541 gctttttcaa atatttcgac tttttccgcc cgatgattgc ccaatatgcc ggaaaaggcg
      601 gcgcaatcga catcctgatg ccgctggggc tttcgtatta caccttccag tcgctcgcct
      661 atctggttta ctgcttccgc gccccgcacg ccgcgcgttt cagctggcac gagctgctgc
      721 tgcacctgag ttttttcccc accgttacct ccggcccgat tatccgcgcc gccgcattca
      781 aaagcgcaga cggcgagcag gcaggcgcat tggcgcaaat ccgtacccgc cgagcgcgtt
      841 cgcccgtccg ccccgcactc gccgtttccc tgattctgct gggtattgcc aaaaaatggt
      901 ggctggcggg gatgctggcg gaaaactggg tgtcgcccgt atttgaaaat cccgcccaat
      961 tcgacggctg gggcgtattg ggcggcgtgt acggctatac cttccaactc tttttagact
     1021 tttccggata ttccgatttg gttatcggca tggcgatgct gctgggcttt aggctgccca
     1081 aaaatttctc cgcaccgctt cgtgctttaa acatccgcgc attttgggac aaatggcaca
     1141 tcagcctttc cacctggata cgcgactaca tctacatccc cttgggcggc agcaaaaaag
     1201 gctttttacg gacacagctc aacctgatgg cggcaatggt gctctcaggc atctggcacg
     1261 gctacggctg gaacttcctc atttggggcg cgctgcacgg cacggcactg gtgctgctca
     1321 acacgggcga ccgctatttc ggacgcgacg cgctatgccg tctgaaatac ttcgcgccgc
     1381 tctcatggct cattaccttc catttcgtct gccttagctt tgtcgtcttc aataccgcaa
     1441 atcccgacga tgcaggcgca gttttcagtg ccctctttgc caatgccaac ggctggaatg
     1501 cgccgcaaca ggcaaacatg ctgttgcttg cctcgtttgc atccgtgatg ctgctctacc
     1561 cttacctgca acgcgctttc gacggcgcgg tcaaaggttt ggaaaaaatc ccgatgtggc
     1621 tgtggtttat ccccgtttcc gccgtcctgc tgctgattat cgtcctcgcc ccctcgggga
     1681 tacccggctt tatttatgcc aatttttaag ggtttggaca tgaaaaactt tctttccctt
     1741 ttctcctcca tactgatgtc tgccctgatt gccgtgtggt tcagccaaaa ccccatcaac
     1801 gcctactggc agcagaccta ccaccgcaac agcccgctcg aaccgcttgc cgcctacgga
     1861 tggtggcgga gcggtgcggc gttgcaagaa aacgcctacg ccctttcaga cggcatcaaa
     1921 gccttcctgt ccggcgaaac gccgccgacg gctcaagacg gcggttcggc agatatgccg
     1981 tctgaagccg ccgcatccga agccgtccct caaaccggtg aaacagaatg gaaacaagac
     2041 accgaagccg ccgccgtccg cagcggcgac aaagtctttt ttgtcggcga ctcgctgatg
     2101 cagggcgttg cccccttcgt gcaaaaaagc ctgaaacagc aatacggcat cgaatccgtc
     2161 aacctcagca aacaaagcac ggggctgtcc tacccctcat tcttcgactg gccgaaaacg
     2221 attgaagaaa ccctgcaaaa acatcccgaa atcagcgtac tcgccgtctt cctcggaccg
     2281 aacgacccgt gggatttccc cgtcggcaaa ctctatctca aattcgcttc cgacgaatgg
     2341 gcgcaagaat acctgaaacg tgtcgaccgc atccttgaag ccgcacacac gcaccgcgtc
     2401 caagtcgtct ggctcggcat cccctacatg aaaaaagcca agctcgacgg acagatgcgc
     2461 tacctagaca aactgctttc ggaacatttg aaaggcaaaa tcatcctgat tcccaccacg
     2521 cacaccctga gcggcgggaa agaccgctac accgactccg tcaacgtcaa cggcaaaccc
     2581 gtccgctacc gcagcaagga cggcatacac tttaccgccg aaggacaaaa actgctggcg
     2641 gcaaaaataa tggaaaaaat cgtttttgaa ccaagtacgc aaccatcaag tacacagcca
     2701 tgaaccccaa acacctcatc gcattttccg ccctattcgc cgccacgcag gcagaagccc
     2761 tacctgtcgc ctccgtcagc ctcgacaccg ttaccgtttc cccgtccgcc ccctacaccg
     2821 atacaaacgg gctgctgacc gactacggca acgcctccgc ctcgccttgg atgaaaaaac
     2881 tccaatccgt cgcacaaggc agcggcgaga ccttccgtat cctgcaaatc ggcgactcgc
     2941 ataccgccgg cgacttcttt accgacagcc tgcgcaaacg cctgcaaaaa acttggggcg
     3001 acggcggcat aggctgggtt taccccgcca acgtcaaagg gcagcgcatg gcggccgtcc
     3061 ggcacaacgg taactggcaa agcctcacca gcaggaacaa caccggagac ttcccgctcg
     3121 gcggcatcct cgcccacacc ggcagcggcg gcagcatgac cctgaccgca tcggacggca
     3181 tagcaagcaa gcagcgcgtt tccctgtttg ccaaacccct gcttgccgaa caaaccctga
     3241 ccgtcaacgg caacaccgtc tccgccaacg gcggcggctg gcaggtactg gatacgggcg
     3301 cggcactgcc cctgaccata cacaccgaaa tgccgtggga catcggcttc atcaacatcg
     3361 aaaatcccgc cggcggcatt accgtttccg cgatgggcat caacggcgca caattaaccc
     3421 agtggtcgaa atggcgtgcc gaccgtatga acgacctcgc ccaaaccggc gccgatttgg
     3481 ttatcctttc ctacggcacc aacgaagctt tcaacaacaa catcgacatt gccgacaccg
     3541 aacaaaaatg gctggatacc gtccgccaaa tccgcgacag cctgcctgcc gccggcatcc
     3601 tcatcatcgg cgcacccgaa tccctgaaaa acacgctcgg cgtatgcggc acacgccccg
     3661 tccgcctgac cgaagtccaa cagatgcagc ggcgcgtcgc ccgtcagggg cagacgatgt
     3721 tctggtcttg gcaaaacgcc atgggcggca tatgcagcat gaaaaactgg ctcaaccaag
     3781 gatgggccgc caaagacggc gtacacttct ccgccaaagg ctaccggcgc gcggcggaaa
     3841 tgctcgccga cagcctcgaa gaactcgtcc gctccgctgc aatcaggcaa taatcggaca
     3901 ggaggcggac ggtatttccg caacaggggg atgccgtctg aaacgcatac cttcatattg
     3961 cttcagacgg catagccacc ccgcgcacgg tttgccggac gcaaccggca ttcgcctcag
     4021 gcatcggaag gacgcaggcg aacctccggc atacggcgca aaggcggcgt ttgatatgcc
     4081 gtctgaaggc aaagatgata aactgccgcc ttccgttttc agacggcata ttgttttcaa
     4141 atgagggcgt tctccgtccg caaccataaa ggaagtttca tgaaccggac ttatgccaat
     4201 ttctacgaaa tgctcgccgc cgcctgccgc aaaaacggaa acggcacggc agtgttcgac
     4261 ggcaaggaaa aaaccgccta ccgcgcgctc aagcaggagg ccgaagccgt cgcggcgtat
     4321 ctgcaaaata tcggcgtgaa gttcggcgac acggtcgcgc tggcggtttc caattccaca
     4381 gaatttatta ccgcctattt cgccatctcc gccatcggcg cggtcgccgt accgatgaac
     4441 acatttttga aaaacagcga atacgcgtat atcctgaacg actgcaaggc gcgcttcctg
     4501 ttcgcctcgg ccggcctgtc aaaagaattg gcgggcttga aggcgcaaac gcccgtcgaa
     4561 aaaatcattt ggacggacaa aagccgtccg accggcgaaa cggcggaagg cgatgccttt
     4621 tttgaagacg tgcgccgctt ccccgaaaaa cccgacttgg gccgccaacc ccggataaat
     4681 gatttggcac acatcatcta cacctccggc acgacggggc atcccaaagg cgcgctaatc
     4741 agttacgcca acctgttcgc caacctgaac ggcatcgaac gcatctttaa aatttccaag
     4801 cgcgaccgct ttatcgtttt cctgccgatg ttccacagct tcacgctgac ggctatggtg
     4861 ctgctgccga tttatatggc gtgttcgatt attttggtca aatccgtttt tccgttttcc
     4921 aacgttttga aacagacact gctcaaacgc gcgaccgtgt ttttgggcgt acccgcgatt
     4981 tacaccgcga tgagcaaggc gaaaatccct tggtatttca gatggttcaa ccgcattcgc
     5041 ctgtttatca gcggcggcgc gcctttggcg gaacaaacca tcctcgattt caaagccaag
     5101 ttcccccgcg ccaaattgct ggaaggctac ggactgagcg aagcctctcc cgtcgtcgcc
     5161 gtcaatacgc ccgagaggca aaaagcccgc agcgtcggca tccccctgcc cggtttggaa
     5221 gccaaagccg tcgatgaaga attggtcgaa gtgccgcgcg gcgaagtggg cgaactgatc
     5281 gtcaggggcg gttcggtgat gcggggctac ctcaatatgc ctgccgccac cgatgaaacc
     5341 atcgtcaacg gctggttgaa aacgggcgat ttcgttacca tagacgaaga cggctttatc
     5401 tttatcgtcg accgcaaaaa agatttgatt atttccaaag gtcaaaatgt ctatccgcgc
     5461 gagattgaag aagaaatcta caaactcgat gccgtcgaag ccgccgccgt catcggcgtg
     5521 aaagaccgtt atgccgacga ggaaatcgtc gccttcgtcc aattgaagga aggtatggat
     5581 ttgggcgaga acgaaatccg ccgccacctg cgtaccgtgc tggcaaattt caaaatcccc
     5641 aaacaaatcc actttaaaga cgggctgccg cgcaacgcta cgggcaaggt attgaaacgg
     5701 gtgttgaagg agcagtttga cggaaacaaa tgaacgccgt gccgtccgaa gccccgtccg
     5761 gcaaaaaaat gcggtgaatc tgattcaccg catttttttg acaaacaccg gagggagggc
     5821 atttactctg ccaattccac ctgctcgtgc gccatcagtt cctgaccgac atcatccaac
     5881 aacatcaaat aacgttcgta gtttttcaaa atgtcggcaa tcagctcgtc cttgttcata
     5941 tactgcacat cgtacccgac gcgcccgtcg aaaaaataag cgtagggttt gtaagttgtc
     6001 tgatgccgga tatgcggcag cttgccgtcg ttaatcaact ggtcggatac atcctgcccg
     6061 acagacttaa tcccgtacat aaaatcgcgc atcgtctctt tccgaatgac gaactcgatt
     6121 gcgggctcgt cccgatgaaa cattttatcg acccggacgc tcaagccgta ttcttccgaa
     6181 agctcccgtt gcaactcgtg catagcgggc gatgcagtct gtttgaggaa ttttaaaata
     6241 tcctgctcct gcgtctggct cattatctgc accagccgtt ctttccactt gccgcccgtc
     6301 caaaatacac tggtagggtt aacccgggtc tcaaaatatt tcttatccgc actcaagcct
     6361 ttccacaggc tgaaacacat tatcagcatc agcagggcaa acggcaggga aacaatcagg
     6421 gtcatagact gcaggttgcc gagtccgccc gagcgcatca gcaaaacggc aacggcagac
     6481 atcagcacgc cccacataac cgcctgccac cgtggcgcgc tcaagccttt gtcccgagag
     6541 gtaatattgt tcaggacata aatcccggaa tcggcagaag ttacaaaaaa cagagaaatg
     6601 accagcaggc tgacgatgct cgtcaattcg ggcaggggga ggtaattaaa gaatttaaaa
     6661 agcagcgttt ccggagagga ggtcatcttt tcgagcattc cccccgcaac cccgtcattc
     6721 agccaaatcg ccgtattgcc gaagacggta aaccacaaaa cgccgaacag gccggggatg
     6781 agcaaaaccc cgaagacaaa ctcgcggatg gtgcgcccct ttgaaatgcg cgcgataaac
     6841 aaacccacaa acggcgccca agaacaccac cacgcccaat aaagcaccgt ccaagattca
     6901 aaccacggct tgtgttcccg ttcgtacgca taagttttaa aactgaggcg caccagattt
     6961 ccgaggtagt tccctatgtt gtcgccgaat gccgacaaca ggtaaacagt gggtcccgcc
     7021 gccaaaacaa aaaacagcag caaaaacgca aggcccaggt tcaactcgct caacaccttc
     7081 acgcccttcc ccacgccgga tattgccgaa acgacggcga gggacatgac ggcggcgata
     7141 atcaaaacct gcacgctgaa gctgttttcg gcaatccagc ccatttcctg caatccggcg
     7201 cccagttgcg aagccccgaa ccccaatgtg gtgatgatgc cgaaaaaagt agcaagcaac
     7261 gccataatat caatggcatc gccgaacctt ccggaaattt tttctttcaa cagggggtaa
     7321 aaacaagaac gcagggcaag cggcagcttg tagcggaaac cgaaataagc caaagccaat
     7381 gcaatcgtac cgtacaccga ccaagcgtga acgccccaat ggaacaccgt gtgcagcaat
     7441 gcctgctgct gcctgtgttc cggcgtgccg gccgtaatgt ccgaaaaata atgcatcaac
     7501 ggctctgcca cgccgaaaaa catcagaccc acgcccatcc cggccgcaaa cagcatcgcc
     7561 agccacgaca ggaagccgaa ttccggcaca tcttcatccc gtccgagcct gatgtttccc
     7621 aaactgctga ccgagagtat cagcaggaaa cccagaaaaa tggaaaacgt taaaacataa
     7681 aaccagctga actcggtaaa aatgacttct tttgcccgat cgagccacat ctgcacctga
     7741 tccggcacgg ttaaaaccaa taccaccaaa acacacacaa aaaacaaagt cgtcaaaata
     7801 accatcggat taaatgacgt tcggcgttct ataaattcag acagggacaa accttctcac
     7861 tcctttgtta aaaacagaca aacccggtca tcgggcaaag cggtcaaaac ctgccggtaa
     7921 aataccggct tccggatgac gaaatgcaca aaccggccga aattgttata atcgaaaaaa
     7981 ttaaaaatca atacggatta ttctatcaga ttgatttatc gatatttatt atatcattaa
     8041 tatagtttga tttcaaaccg gcggaaaatg cgccgcccac cgcaggcgcg ccctccccgc
     8101 tatcggggcg tttaacgccg gattgccgat gcggtacaat ggctgatatg aagaaaatta
     8161 cccctcaaaa cctgcgcccc ctgctttcgg aaagcttggg acataccgat tttgtcaacg
     8221 tcctcaacgc actgattaaa tttttgcgcc gtggcggcaa aaaatgtgcg ggggaacgtt
     8281 tcgacctgat tatcgacaca ttcaaacaag acagggaatt actgtcccgc ttcagccggt
     8341 gtttttacat ttggctcgcg caaatacaca tttatccggc actcatcaaa ctcggcatct
     8401 tctcgcgcca cagctttgcc cgggaaatgg gcatacgcat ctacgaacgc ttcagcccgt
     8461 catataaaga ttttgccaac ttgggcgaag tcttccttta tcttttccat tccgaaaacg
     8521 acgacaaatg gctgcaaacg ctcaatatcc gccaatggct ggttttatac gaactcatcc
     8581 ggagccacgc cgagccgtcc aaattgcaga cggcgggcat ccgccttgcc gatgcgcgtt
     8641 tgcgcgccat cgaaatgctg tctgtctgga cggcatccga agccatcgaa cccgacctca
     8701 tccgcatcgc cccgcgcctg ctggaagccg attcttcctt cgtcgccctc caacgcgaaa
     8761 ccgccaaact ggtcgaacac taccgcaacg gcaccacgcc ttacgacacc gcccacctcg
     8821 aagtgatgtt cgaccaatgt ttcagccaga ttgactattt gcgccgcaaa gggacgggcg
     8881 ccggctccgg ttcgtcggtc aaagtcgccc acctgctcga acggctccgg cagaccgtag
     8941 accgtctgaa gctgctcacc gacatccaaa ccggcgccgg caacagcaac cgcctgacca
     9001 tcgcgctgat gaactccctc atctacgcgg cggtcgaaca atacagcacc cgccacctgc
     9061 gccgcagcag catccgtatg ctcgcccgca gcattaccga aaacaaaagc caccacggcg
     9121 aacactacat cacccgcaac cgcaaagaat atttcaaaat gttctactcg gcggcaggcg
     9181 gcggcatcat catcgcccta atggcgctgc tcaaaatccg catcggctca ctcggcctca
     9241 gccccttcct cacttccttg tcggctgggt tcaactacgg catcggcttt atgatcatcc
     9301 atatgctgca ctgcaccgtc gccaccaagc agcccgcgat gactgccgcc agctttgccg
     9361 aacaggtcga tctcaacgaa ggcggcaaag cggtggacaa caaactcgcc aagctcctca
     9421 tcgacgtatg ccgctcccaa agtgtcgccg tcttcggcaa cgtttccatc gccatccttt
     9481 tggcgtgcgc catatcgttc ggctatgccc atctgtaccg gctgcccata ctcgatgccc
     9541 acaccgccgc ctaccagttc aaatccatag acatcatcgc ttacccgacg ctgtggtatg
     9601 ccgccattgc aggtctgtgg ctgttctgct ccggcatcat cgcaggtttt ttcgacaacc
     9661 gcgccgacta cctcaacctg cgccaacgcc tgcccttcaa ccccttgctg cgtaaaatca
     9721 tgcgccccgg gccccgccgc gtcctcgccg cctacatcca caaacactac ggctcgctgg
     9781 tcggcaactt catcttcggg atgctcttgg gtatgaccgg ctatttcgga cacctcctcg
     9841 ggctgccgct ggacatccgc cacgtcgcct tttcctccgc caacctcggc tatgccgccg
     9901 tcagcggcaa cgtcggtttg ggcacgttcg tactcggcat tttcagcgtc ctcgccatcg
     9961 gcctggtcaa cctctgcgtc agcttcagcc tcgccctctt cgtcgccctg cgctcgcgcg
    10021 gcacgaaaat cggcagcatc cgcaatctga ttaaaagttt ttggaatcag attaaaagca
    10081 atccctgcat acttttcctc ccgcccgcca aagaacaggg acatcctcct tcggacaagc
    10141 cttgaccggc aatgccgtct gaagcgggat tcgccccgaa taccgccctg atgcgggaaa
    10201 tccccataaa aggatgcaaa aatgccgtcc gaaccgaaac gtggttcaga cggcatttta
    10261 aaaaacatta caatcccggg ccgccaagcg aattggcaat gtccctgacc gccgttacat
    10321 acatccggct gtggttgtac tgccataccg tataaaaatt gttcaagccc aaataatatt
    10381 caaacacgcc cggtgcggtt tccagtttga acaaaaccgc cttttcatca tctgcaagct
    10441 cttcgccggg gatgatgccg tacgccttca aatccgccac cgtccgcgtc agggcggttt
    10501 tttcgccaat gattgcctga acatccgcac ccggcgccaa tgttgcagac accagcattt
    10561 tcccgcccgt gcgccaaccg tgctgcttca tataattggc aaccgatgcc gcgacatcgc
    10621 cgacgttgcc ccatatgtcc cgatgtccgt ccccgtcata atccaccgcc catttccggt
    10681 agctcgaagg cataaattgc ggcatcccca ttgcgcccgc atagctgcct ttaaaggcga
    10741 aaacatcgcc gccttcttct tttgccagct ttaaaagctc gaccaattct ttttggaaaa
    10801 acccggcgcg gcgggggtaa tcaaagccta aggtcgccaa tgcgtccgcc acacggaaac
    10861 tgcccgtatt tttgccgtaa ttcgtttcaa tcccgataac cgccacgata agttcggcag
    10921 gcacgccgta tttttgcgcc acatcatcga taagcgcgcg gttttccgca taaaaccggc
    10981 gcgcgccgcg aaatttcgcc ttgcccgaat ttcccgtgcg gaacacatac cacggacgcg
    11041 atgtggaggg gcggtgcata atcttgacga tgtccgcctt gtaagccgct ttgtcaaaaa
    11101 aatcctgcca ttccgcccgg gaaaaatccc ctttcccgac ttcatcgtcc acaaaacggc
    11161 ggacatttgc attggcggca aacccgctgt cggataccgg tacggctgcc gcgtcaaata
    11221 cggctgccgc gtcaaacgcg gggcggcttt cttttttcat ttcaaccgcg cggggggctt
    11281 gggcttcatt tgcccggggt gggcgtgcct ccatcgccgt acaggcagac aaagccgcca
    11341 aacaaattgc cagcggcagt atttttctct ttttcataaa tatgttccga acaaataggg
    11401 taagtgggaa agcggcacaa gggggcggcg cgaaatgcgg catccgccgc aatcggcggc
    11461 tttgcggaat gccgcacgtt gcctcttgca ccgcccgaaa tccgtatgtc gtcgccgaaa
    11521 atgccgtctg aaggcacttc ccctttcaga cggcattgcc tgccgccgta tttgcccgct
    11581 acccgcaata tcggcagtcc aatatatctt tgcggatgtc gttcagcagg aaggctgcgg
    11641 tgtcttcgtg tttcgcctgt acgaagacaa agagtcgggc gatgattttg ccgataaaga
    11701 ctttttgcag ggcgcaggca tcggtcaggg tgtgttcctg caggaagctg cggatgtctt
    11761 caggcaaagt gtagtcgcgg gcgacggcgg caaagcgggc atcggtttgc aggcggtcga
    11821 gcagggtttg gtttttgtcc aacttcatgc ctgcttcttt ttcttcggcg gtggcgcgga
    11881 caaactggtc gtaaatttcg gcataaagca tatcgttcgg gccttcaaaa atcgtgaagg
    11941 ggcggatgtc gatagcgata ttgccggcgg tgtgtccgcg ttcaaaaccc ttcgcaccca
    12001 agagtttttg caacatttgc gcggcggcgt aagtgtattc cgtggcgagg gttttgacga
    12061 tgttcgcctc catcagctga tgggcgacgg gggcaacagg cgaaacggaa tggcagacgt
    12121 agcggtaaag aatctcggaa acctgatggc ggcgccggat ttcgcggcgt tcgtaatcga
    12181 cgaatttgat gtcgttgcgg acgtatcgtt ccagattttc aaggatgtat tccataatgc
    12241 cgtgcgtcat gccgatcagt tgcaggcggc tgcggataaa gatgttttgg aacgcgcgca
    12301 aaccggcagc gtcgctctgg gagagtttca tcacggcggt tgcaggcatt tcggcatcga
    12361 tgcggttgac ggcgtaacgg acggcgcgca agccttcgga tgcgagggtt tcgcagcgga
    12421 tgtatgtttt ggggatgagc agcaggtcga tgactttggc gagtttgccg tttttgcgct
    12481 ctttggcggc aacgaggagg aagtcgcttt gcgagttgcc ctgccagtat ttcgcggcgt
    12541 tgacgtaaat ggtttgtccg tcgatatatt cgtagtagga ctgcatttcg cgtgcaatcg
    12601 ccgcgccgga ggtttcgggt tcggtaacac ccaaaccgcc gccctcgcct ttgaaaatca
    12661 tctccaaacc ttgcgcgact tgcgcttcat cgccgaactc ttgcagtggc tgcaacacca
    12721 gcgcgccttc gatgccggta cgcagcgtaa cgggcacgcc gtaatgcccc gcaatccgca
    12781 ggacttcttg gatttcaaac tggctgccct tgcgcccgcc gtattttttg tcgaggaagg
    12841 gcaacagcaa acccgcctgc ttcaaggcaa gccatttgtc ttcgggcagg tatcgcatca
    12901 ggtcgatacc gtctgaaaaa atgcggcgga atgcggattc gatgtgcttt aaaaaagcag
    12961 ccgtgtccat agttgacggc tgcgcgctcg gttcggtgtg tatcatcggc ttcctctgtc
    13021 ggttcccatt aatcggcggc cggtcaaacc gcctgccaca gtttagagtt gattttctaa
    13081 actttaccac aaagtgcgcc gggcaacaat ccgccgacct ttcagacggc atcgcgtccc
    13141 ctcccgtgct aaaatgaccg tttgcatcac tgtccgccga ttgccgcact atgacctacc
    13201 ccatccccaa accccgtgaa aaatcccgtt ggcccaatct ttcgcaaggc tcgctgccct
    13261 tggctttggc gcgttatctg ccgcacaagc ggctcaaggt cgtgctgacc caagatgcgg
    13321 aacaggcgtt gcgccttcag acggcatggc ggtttttccg tccgcacgac acggcggtgt
    13381 tcctgccgga ctgggaaacg ctgccttacg agcgtttttc gccgcatcag gatttggtgt
    13441 cggagcggct gtcggcgttg tggcagatta aaagcggcgc ggcggatgtg ttgttcgtgc
    13501 cggttgccac ggcgatgcag aagctgccgc ccgtgccgtt tctggcaggg cgcacgtttt
    13561 ggctgaaaac ggggcagact ttggatatag gccgtctgaa aagtgatttg gtggatgcgg
    13621 gctacaacca tgtttcccac gttgtcgcgg cgggcgaatt tgccgtgcgc ggcggtatag
    13681 tcgatttgtt cccgatgggc agcgaaatgc cgtaccgcat cgatttgttt gacgatgaaa
    13741 tcgacagcat caaaaccttc gataccgaaa cgcaacgcac catttccccc gtttccgaaa
    13801 tccgcctgct gccggcgcac gagttcccca ccgacagcga ggcgcaaaaa atcttccgca
    13861 gccgcttccg cgaggaagtc gatggtaatc cgaacgatgc ggctgtgtac aaagccgtca
    13921 gcaacggtca tttcggcgcg ggcgtggaat actacctgcc gctgtttttt gaaaacgagt
    13981 tggaaacgct gtttgactat atcggcgaag atgcgctgtt tgtctcttta gacgatgttc
    14041 atgccgaggc aaaccgtttt tggagcgatg tcaaatcgcg ttacgcgatg gcgcagggcg
    14101 acgaaaccta tccgcctttg cttccacagt atttgtatct ctctgccgat gtgttcgcag
    14161 gccgtctgaa aaactacgga caggtgctgc ccgatgtttc cggcaaggaa tacaccctgc
    14221 ccgaccttgc cgtcaaccgc caagcagatg agccgttgca ggcattgaag gattttcaga
    14281 cggcgtttga cggacggatt ttgctgtgcg ccgaaagttt gggacggcgc gaaactatgc
    14341 tcggtttctt gcagcaaaac ggtttgaaag ccaaacccgt gtccgactgg cagggctttt
    14401 tatcggcaca cgagccgctg atgattacag tggcgccgtt ggcatacggg ttcaaactgg
    14461 gcggactgca atcgccgaac caacagcaac ctactccctc ccccgtgggg gagggttggg
    14521 gagagggcaa agcagttgcc gctcaaactg aattttccgc agccgcaata aaccctctcc
    14581 ctagccctct cccacaggag agggaacaaa gtgcagccgc cgtttcagac agtctgaaag
    14641 cagccgccgt ttcaaccgaa agcagcctgc ccctcggtac aagtaatctg cacgggcaaa
    14701 tccgacagca acctgcccct tcccccgtgg gggagggttg gggagagggc aaagcagttg
    14761 ccgctcaaac cgaatttccc gcatccgcaa caaaccctct ccctagccct ctcccacagg
    14821 agagggaaca aagtgcagcc gccgtttcag acgacctgaa aaccaaaagc agcctgcatc
    14881 ccgtcgcaaa taatctgcac gggcaaatcc gacagcaacc tactccctcc cccgtggggg
    14941 agggttgggg agagggcaaa gcagttgccg ctcaaaccga attttccgca gccgcaacaa
    15001 accctctccc tagccctctc ccacaggaga gggaacaaag tgcagccgtc gtttcagaca
    15061 gtctgaaagc agccgccgtt tcaaccgaaa gcagcctgcc ccccggtaaa agtaatctgc
    15121 acgggcaaat ccaacagcaa cctgccccct cccccgtggg ggagggttgg ggagagggca
    15181 aagcagttgc cgctcaaagt gccatcgccg tcatcaccga atccgatctc taccaatacg
    15241 tcgcccgttc gcgcatccac aaccgccgca agaaacacgc cgccgtttca gacgggctgt
    15301 tgcgcgacct tgccgaaatc aatatcggcg accccgtcgt gcacgaagaa cacggcatcg
    15361 ggcggtatat gggcttggta acgatggact tgggcggcga aaccaacgaa atgatgttgc
    15421 tcgaatacgc aggcgaagcg cagctttatg tgcctgtttc gcaactgcat ttaatcagcc
    15481 gctactccgg tcaggcgcat gaaaacattg ccctgcacaa gctcggcagt ggcgcgtgga
    15541 acaaggcgaa gcgcaaagcc gccgaaaaag cgcgcgacac cgccgccgaa ttgctcaacc
    15601 tctacgccca acgcgccgcc caatcgggac acaagtttga aatcaacgag ttggactatc
    15661 aggcgtttgc cgacggcttc ggctacgagg aaaccgaaga ccaggccgcc gccatcgccg
    15721 ccgtgattaa agatttgacg caagcgaagc cgatggatcg ccttgtgtgc ggcgatgtcg
    15781 gcttcggcaa aaccgaagtc gccctgcgcg ccgcgtttgt ggcggtgatg ggcggcaaac
    15841 aggtcgccgt acttgctccg accacgcttt tggtcgagca gcacgcgcaa aacttcgccg
    15901 accgtttcgc cgatttcccc gtgaaagtcg ccagcctttc gcgtttcaac aacagcaaag
    15961 ccaccaaagc cgcgctggaa ggcatggcag acggcacggt cgatattgtt atcggtacgc
    16021 acaaactggt gcaggacgac atcaaattca aaaacttagg tttagtgatt atcgacgaag
    16081 aacaccgctt cggcgtgcgt cagaaagagc agctcaaacg cctgcgcgcc aatgttgata
    16141 tccttaccat gaccgccacg ccgattccgc gtactttaag tatggcgttg gaaggactgc
    16201 gcgacttctc gctgattacc accgcgccca gccgccgcct cgccgtcaaa acctttgtca
    16261 aaccctttag cgaaggcagc gtgcgcgaag ccgtgttgcg cgaactcaaa cgcggaggac
    16321 aggtattttt cctgcacaat gaagtagata cgattgaaaa tatgcgcgag cggctggaaa
    16381 ccctgctgcc cgaagcccgc atcggcgtgg cgcacggaca actgcgcgag cgcgagctgg
    16441 aacaagtcat gcgcgacttt ttgcagcaac gatttaacgt gttgctctgt tccaccatca
    16501 tcgaaaccgg tatcgatatc cccaacgcca acaccatcat catcaaccgc gccgacaaat
    16561 tcggactggc gcaactgcac cagcttcgcg ggcgcgtcgg ccgcagccat caccaagcct
    16621 acgcctacct gctcacgccc gaatacatca ctaaagacgc agaaaaacgc ctcgatgcca
    16681 ttgcggcggc agacgaactc ggcgcaggtt ttaccctagc catgcaggat ttggaaatcc
    16741 gtggtgcagg cgaaatcctt ggcgaaggac aatccggcga aatgatacag gtcggcttca
    16801 cgctctacac cgaaatgctc aaacaagccg ttcgcgacct caaaaaaggc cgccagcccg
    16861 acctcgacgc accgttgggc atcaccaccg aaatcaaact gcacagcccc gccctgctgc
    16921 ccgaagatta ctgccccgac atccacgaac ggctcgtcct ctacaaacgc ctcgccgtct
    16981 gcgaaaccgt gcaacaaatc aacaccatac acgaagaact cgtcgaccgc ttcggcctgc
    17041 ccgaacaacc cgtcaaaacc cttatcgaaa gccaccactt acggcttatg gcaaaagaat
    17101 tgggtatcga tgccattgat gcggccggcg aagcggtaac ggtaaccttt ggtaaaaaca
    17161 ataatgtcga tccaaccgaa atcatcctgc tgattcagaa cgacaaaaaa taccgccttg
    17221 ccggcgccga taagctgcgg tttaccgcag agatggaaaa tatcgaggtc agaatcaaca
    17281 ccgtaaaaaa cgttttaaaa accttgcaaa acagatgcct gcccaaataa agccgacacc
    17341 gcaatgccgt ctgaaacacc gttttccttg tccgaaagcc gccattatga atttgaagga
    17401 aactccacta taatacggca ttcagatttc cagacggcat cgcgcccgtc aaaccgcaca
    17461 caaaccaaaa ggaaatacat gttccgtacc atgcttggcg gaaaaatcca ccgcgccacc
    17521 gttaccgaag ccgatttgaa ctatgtcggc agtattaccg tcgatcaaga cctgttagac
    17581 gcggcaggca tctaccccaa cgaaaaagtc gccattgtca acaacaacaa cggcgaacgt
    17641 tttgaaacct ataccattgc agggaaacgc ggcagcggcg tgatttgtct gaacggtgct
    17701 gcagccaggc tggtacagaa aggcgatatc gtcatcatca tgtcttacgt ccaactctcc
    17761 gaacccgaaa tcgccgcaca cgaacccaaa gtcgtcttgg tagacggaaa caacaaaatc
    17821 cgcgacatca tctcctacga gccgccgcac accgtgctgt aattccgcaa acggacatcg
    17881 attatggata ttaaaatcaa cgacatcacc ctcggcaaca actcgccctt cgtcctattc
    17941 ggcggcatca acgttttgga aagcttggat tccaccctcc aaacctgcgc gcattacgtc
    18001 gaagttaccc gaaaactcgg tattccctat atctttaaag cctctttcga caaggcaaac
    18061 cgttcctcca tccattctta tcgcggcgta ggcttggaag aaggcttaaa gatttttgaa
    18121 aaagtcaaag cagagttcgg catccccgtc attaccgacg tacacgaacc ccatcagtgc
    18181 caacccgtcg ccgaagtgtg cgatgtcatc cagcttcccg cctttcttgc gcggcagacc
    18241 gatttagtgg ttgccatggc aaaaactggc aacgtcgtca acatcaaaaa acctcagttc
    18301 ctcagcccct ctcaaatgaa aaacattgtg gaaaaattcc acgaagccgg caacgggaaa
    18361 ctgattttat gcgaacgcgg cagcagcttc ggctacgaca acctcgttgt cgatatgctc
    18421 ggtttcggcg tgatgaaaca gacttgcggc aacctgccgg ttattttcga cgttacccat
    18481 tccctgcaaa cccgcgatgc cggttctgcc gcatccggcg gtcgtcgcgc acaggctttg
    18541 gatttggcac ttgcaggcat ggcaacccgc cttgccggtc tgttcctcga atcgcacccc
    18601 gatccgaaac tggcaaaatg cgacggcccc agcgcgctgc cgctgcacct tttagaagat
    18661 tttttaatcc gcatcaaagc attggacgat ttaatcaaat cacaaccgat tttaacaatc
    18721 gagtaacacg gtttcgcctt atgatgcaga ctttccgaaa aatcagccgg tatgtcgcaa
    18781 ccttgtggct cggtatgcag attatggcgg gttatatcgc cgcaccggtg ctgttcaaaa
    18841 tgctgcccaa aatgcaggcg ggcgaaattg ccggcgtatt gttcgacatc ctctcttgga
    18901 gcgggcttgc cgtttggggc gcggtactgg ctgccgcctt tgccgcccta acccggcggc
    18961 aaaccgccct gctgcttttt ttattgtccg cccttgccgc caaccgattc ttgattacac
    19021 ccgttatcga ggcactgaaa tacggacatg aaaattggct gttgtcgttt gtaggcggat
    19081 ccttcggaat gtggcacggc atttccagta ttgtttttat ggcaaccgcc ctactttcag
    19141 cagttttaag ttggcggctt tccggcaaag atgccgtctg aagccctccc atttttttac
    19201 ctcccttcac ttcacttgga gaacattcat gagcgcaatc gttgatattt tcgcccgcga
    19261 aattttggac tcacgcggca accccacagt cgagtgtgat gtattgctcg aatccggcgt
    19321 aatgggacgc gcagccgtac cgagcggcgc gtccaccggt caaaaagagg ctttggaact
    19381 tcgcgacggc gacaaatccc gttattcggg caagggcgta ttgaaggcgg tcgaacacgt
    19441 caacaaccaa atcgcccaag ccctcattgg tatcgatgcc aacgagcaat cttatatcga
    19501 ccaaatcatg atcgaattgg acggtactga aaacaaaggc aatttgggtg cgaatgcgac
    19561 tttggcggtt tctatggcgg ttgcacgcgc cgctgccgaa gactcaggcc tgccgcttta
    19621 ccgctacttg ggcggcgcag gcccgatgtc cctgcccgta ccgatgatga acgtcatcaa
    19681 cggcggcgaa cacgccaaca acagcctgaa catccaagag tttatgatta tgcccgtcgg
    19741 cgcaaaatct ttccgcgaag cgttgcgctg cggtgcggaa attttccacg ccttgaaaaa
    19801 actgtgcgac agcaaaggct tcccgaccac agtcggcgac gaaggcggtt tcgcccccaa
    19861 cctgaacagc cacaaagaag ccctgcaact gatggtcgag gcgaccgaag ccgccggcta
    19921 caaagcgggc gaagacgtat tattcgcatt ggactgcgcc tccagcgagt tctacaaaga
    19981 cggcaaatac cacttggaag ccgaaggccg ctcctacacc aacgcggaat ttgccgaata
    20041 tctggaaggc ctggtcaacg agttccccat catctccatc gaagacggca tggatgaaaa
    20101 cgactgggaa ggctggaaac tgctgaccga aaaactgggc ggcaaagtcc aactcgttgg
    20161 cgacgacctc ttcgttacca acccgaaaat ccttgccgaa ggcattgaaa aaggcgtggc
    20221 aaacgcacta ttggtcaaag tcaaccaaat cggtactttg agtgaaaccc tgaaagccgt
    20281 cgatctggca aaacgcaacc gctacgccag cgtgatgagc caccgctccg gcgaaaccga
    20341 agacagcacc attgccgact tggcagtcgc caccaactgt atgcagatca aaaccggttc
    20401 tttgagccgt tccgaccgca tggcaaaata caaccaactg ctgcgtatcg aggaagaatt
    20461 ggcggaagcc gccgactacc ctggcaaagc cgcattctac caactgggca aataagaaaa
    20521 ggttaaggta tgaagtgggt aactgtcgtt ttatccttcg cacttgtctg ttgccaatac
    20581 agcctctggt tcggcaaagg cagcatcgga cgcaacagca gtctgagaga acagattgcc
    20641 gttcaagaag aaaaaaacca gacactcgcc ctacgcaatc attcccttgc cgccgaagtc
    20701 tatgatttgg aaaacggtca agaagccatt tcggaaatcg cccgggtaga actgggttat
    20761 atccaagacg gtgaaacctt ttaccgactc atcaggcata accggtaata ccgtcaaaaa
    20821 gccgtccgaa ccaatgttcg gacggctttt atttcaacaa actgtcagac agcccctcat
    20881 cctcccccga caaaccgcaa tccagcctga catccccctc gatgcaacag cagcacggca
    20941 gtatctcgtc ccgccccaaa aaagccaaag gcggctcccg ataagtaacg cttccctcca
    21001 aaatcttcac ccggcacgat ccgcaatatc cgcttcggca ctgatattcc accatatgcc
    21061 ccgtccgttc caagccttcc aacagggtct cgccctccaa gagctcaaaa aaacccttat
    21121 tcgtaccaat gcgcgccatt tccgaccaat caaa
//
LOCUS       DAWIVB010000015        21124 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWIVB010000015 DAWIVB010000000
VERSION     DAWIVB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.015"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(23..714)
                     /locus_tag="WIX60_000391"
                     /pseudo
     CDS             complement(23..714)
                     /locus_tag="WIX60_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(973..1716)
                     /locus_tag="WIX60_000392"
     CDS             complement(973..1716)
                     /locus_tag="WIX60_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304714.1"
                     /translation="MLAIYSINILIYNEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKH
                     KCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSISYINTERNQSEFANPIYVQ
                     TSATKIATVMAMMITPVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQAEMKETQGEFV
                     PIIAAAAFGGALGTWGYHGANLYNHGKLGTAQGAATAAGIGAATGVAATGLAAAAGGG
                     LAGNLAWRPGIHALGFGANAANNAVNSRK"
     gene            complement(1843..2001)
                     /locus_tag="WIX60_000393"
     CDS             complement(1843..2001)
                     /locus_tag="WIX60_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304715.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1998..2840)
                     /locus_tag="WIX60_000394"
     CDS             complement(1998..2840)
                     /locus_tag="WIX60_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ5304716.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPADERVLLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            2906..3049
                     /locus_tag="WIX60_000395"
     CDS             2906..3049
                     /locus_tag="WIX60_000395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304717.1"
                     /translation="MPSEKYGIRAVKLCGGKCAIIRAVLPLLRSCSANIRAAWTTYNR
                     IPA"
     gene            3079..3999
                     /gene="pyrB"
                     /locus_tag="WIX60_000396"
     CDS             3079..3999
                     /gene="pyrB"
                     /locus_tag="WIX60_000396"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ5304718.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWNCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4009..4467
                     /locus_tag="WIX60_000397"
     CDS             4009..4467
                     /locus_tag="WIX60_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ5304719.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDHFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4532..5197
                     /locus_tag="WIX60_000398"
     CDS             4532..5197
                     /locus_tag="WIX60_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304720.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5260..6477)
                     /locus_tag="WIX60_000399"
     CDS             complement(5260..6477)
                     /locus_tag="WIX60_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ5304721.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6619..7164
                     /gene="def"
                     /locus_tag="WIX60_000400"
     CDS             6619..7164
                     /gene="def"
                     /locus_tag="WIX60_000400"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ5304722.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7251..8177
                     /locus_tag="WIX60_000401"
     CDS             7251..8177
                     /locus_tag="WIX60_000401"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ5304723.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSKS
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGAKL"
     gene            8256..9515
                     /gene="rsmB"
                     /locus_tag="WIX60_000402"
     CDS             8256..9515
                     /gene="rsmB"
                     /locus_tag="WIX60_000402"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ5304724.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASTACADAQDLSAWYDGKPFDAVLADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQVLKSGGRMLIATCSVFVEENDGQLQKFLNRHA
                     DAELIESRVLLPNKHQDGFYYALIQKQ"
     gene            9481..10077
                     /locus_tag="WIX60_000403"
     CDS             9481..10077
                     /locus_tag="WIX60_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ5304725.1"
                     /translation="MAFITRLFKSSKWLIVPLMLPAFQNVAAEGIDVSRAEARITDGG
                     QLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNIDY
                     KLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGVEAGETKAEI
                     RLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10077..12197
                     /locus_tag="WIX60_000404"
     CDS             10077..12197
                     /locus_tag="WIX60_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ5304726.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLTVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SETGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            12190..13467
                     /locus_tag="WIX60_000405"
     CDS             12190..13467
                     /locus_tag="WIX60_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ5304727.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKCASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSQ
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13558..14745
                     /gene="dprA"
                     /locus_tag="WIX60_000406"
     CDS             13558..14745
                     /gene="dprA"
                     /locus_tag="WIX60_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ5304728.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14771..15232
                     /locus_tag="WIX60_000407"
     CDS             14771..15232
                     /locus_tag="WIX60_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ5304729.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15311..17617
                     /gene="topA"
                     /locus_tag="WIX60_000408"
     CDS             15311..17617
                     /gene="topA"
                     /locus_tag="WIX60_000408"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ5304730.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17690..18385)
                     /locus_tag="WIX60_000409"
     CDS             complement(17690..18385)
                     /locus_tag="WIX60_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304731.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            complement(18405..18956)
                     /locus_tag="WIX60_000410"
     CDS             complement(18405..18956)
                     /locus_tag="WIX60_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304732.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHFHIQNGAVFFYSYRDV
                     TIVFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIIIYRPNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19075..19452
                     /locus_tag="WIX60_000411"
     CDS             19075..19452
                     /locus_tag="WIX60_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5304733.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19541..20110
                     /gene="rsmD"
                     /locus_tag="WIX60_000412"
     CDS             19541..20110
                     /gene="rsmD"
                     /locus_tag="WIX60_000412"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ5304734.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20233..20484
                     /locus_tag="WIX60_000413"
     CDS             20233..20484
                     /locus_tag="WIX60_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ5304735.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20581..20664
                     /locus_tag="WIX60_000414"
     tRNA            20581..20664
                     /locus_tag="WIX60_000414"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20615..20617,aa:Tyr,seq:gta)
     gene            20695..20768
                     /locus_tag="WIX60_000415"
     tRNA            20695..20768
                     /locus_tag="WIX60_000415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20727..20729,aa:Gly,seq:tcc)
     gene            20778..20852
                     /locus_tag="WIX60_000416"
     tRNA            20778..20852
                     /locus_tag="WIX60_000416"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20810..20812,aa:Thr,seq:ggt)
     gene            20901..>21124
                     /locus_tag="WIX60_000417"
     CDS             20901..>21124
                     /locus_tag="WIX60_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ5304736.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETR"
BASE COUNT         5316 a         5365 c         5744 g         4699 t
ORIGIN      
        1 taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt tctaccgtaa
       61 aacgtgtttg acgaacggga ttcttattaa aaaacatctg atttctaaca aaatcagtat
      121 tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt ttccatgcgc
      181 tcaagaattg tgatttgctc attgagacgt gccccagcga tggatcagcc agcaaaacag
      241 tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat tttaaataca
      301 caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa ccttgtgctt
      361 caaaacccag ttcgggcatt atgcgttgca tatcgtcaaa agaagcacgc atttgggttt
      421 tatccatttt gtctaagatt tccgcttcag aataatgtct gccataaaaa ttattcagta
      481 acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact atgccggaat
      541 ctcgccgtgc tttccaactc cgtacatgga tgttttggta agaagcgggg ggtcaacaaa
      601 cataggccaa gcaaaaacta tatttggggc gaaaccaatc aaagccgcat aatttatcaa
      661 tttataaaga ttttttatca taatatgtat acgcggaata aacgcatgaa acataaaaaa
      721 ataaaatcat atgcaatttt attgcatgaa taaatatgaa taaaatagat aatgatggga
      781 gtaaatacgc catgtatttt ggaagtttaa atttattaat aataaaataa tttatcgtag
      841 cgcaaataaa tcccaaaatt ggaacgatta gaaaaaaaat taatacaccc atcatcatgc
      901 aaactctata ttaataaata gctaactaat tctattgtgg aaattagtta gctaactaaa
      961 agttattaat gattattttc gagaattgac tgcattgttg gcagcattgg caccaaaacc
     1021 tagtgcatga atacccggtc tccatgccaa attcccagcc aatccgcctc cggcagcagc
     1081 agccagccct gttgccgcta ctcctgttgc cgcaccgatt cctgccgcag tagccgcgcc
     1141 ttgcgcagtt cctaatttac catgattata caaattagca ccatgatacc cccatgtacc
     1201 taatgcaccg ccaaaagcag cggctgcaat aatgggaaca aattcacctt gtgtttcttt
     1261 catttcagcc tgtgataatt gaattgcttt cacattttgg ctgtcaaaaa cttggctgtc
     1321 taaattttgc gccattacag gtgtaatcat catagccatt acaggtgtaa tcatcatagc
     1381 cattacagtt gcaattttcg ttgcgctggt ttgcacataa ataggattag caaattcgct
     1441 ttgattgcgt tcagtgttga tgtagctaat actgctttct agtttgaatt tacccttgtc
     1501 agtcaataat tcttccaaac ttaaaggtaa gtcagcataa gcaatttggc tggcaacagt
     1561 caaaataaaa tctattagac atttgtgttt ttgcatcatt tcgtttgatt ttctagtttt
     1621 tgagaatgat acaaagtttt ttacaaagta aagagtcact ctgaaaaaca ttttttcatt
     1681 ataaatcaaa atattgatag aataaatagc gagcatcgat tcacggtgta ctttagtgca
     1741 aagcgtacaa cgcgagcctg aaccaccagc cgcaacagga aaagaaagcc gatagagtgc
     1801 aatgcttgcc aacgtgcaag cgagcttgca agaacgcttg gctcaacgag agcaggcaag
     1861 acagaaagca gaaagcagga taggagcggt aacgcaaagg tctcgggctt tgatttcgcc
     1921 gtaaaccctg ctgccgcctt gtccggaaag ggtgcaggcg gcgagtgccg acagggtgca
     1981 gatggggagg ggggttttca tttggggtcg caacggaagt ggtatgcgca gatttcaaaa
     2041 ccgtttttga aatacaggcg gtgcgcgtcg gcacggtcgt ggttgacgtg gacgttgagg
     2101 tggattttgg ttacccctgt ttccgcgccg attttgcgga cttcttccaa aaggcgcgag
     2161 gcgtagcctt tgcggcggct ttgcggcagg gtaacgatgt catcgatgtg gatgtggcgg
     2221 ccgctggcga gggtgcaggc ttcgcggaag ccgcagacgg cgacggcatt gtgtttgcct
     2281 tcttcaaaaa tacccagcag gcggtagcct tgggggcgtt ggactttgtt gatctgttcg
     2341 gtaaagcggt tgatgtcggt cagggcggaa cgcaaaacgc tcaaggctgc aaaggcggtg
     2401 gcggtgtcgt ccgcgccgat ttcgcgcaaa acgtaggatg cgcccgaggc ggtctgttcc
     2461 tgtgctttct cggcggcgtg tttttcttcg attgcctgtg ccagcaggac gcgctcgtcg
     2521 gcagggtcgt tttgtccgcc ctgttcgcgt tcttcgagca gggctttgca gtcgatgacg
     2581 cgcaggtcgt tgtcggcggc aaagtccatc aggaagcgga acatctgcgg attgtcggtt
     2641 tccagttttt tatcgacggc gacgcagcgg atgttgtcca ccagtatggg gcgcacccat
     2701 ttcgaatagg aaagcctgtg gtctttggtg aacgaggaca gaatgccgca caggcgttcc
     2761 gcccagtcgc tgggacggaa aatcttgccg gaactcgttg tgccgtggat gacgacttcg
     2821 taggggttgc agactaacat ggcggcttcc tgaaaagaaa tgtctggctc gattatacct
     2881 tatgcttatg caggcgtgtt tggatatgcc gtctgaaaag tacgggattc gtgcggtaaa
     2941 actttgcggc ggcaaatgtg cgataatacg cgccgtattg ccgcttttgc gaagctgttc
     3001 cgcaaacata cgggcggcgt ggacgacgta taaccggata cccgcctgac gcgggttttt
     3061 tacggaaggg gggcaaaaat gcctaatccg ctttacagac agcatatcat ctccatttcg
     3121 gatttgtcgc gcgaacagtt ggaatgcctg cttcagacgg cattgaagct gaaggcgcat
     3181 ccgcgcggcg atttgttgga aggcaaactt atcggttcgt gcttctttga accgtccacg
     3241 cgcacgaggc tgtcgtttga aacggcggtg cagcggctgg gcggcaaggt catcggcttt
     3301 tcggacggcg cgaataccag tgccaaaaaa ggcgagacgc ttgccgatac cgcccgcatc
     3361 atttccggct acaccgacgc aatcatccaa cgccacccca aagacggcgc ggcgcgcgtg
     3421 gcagcggagt tttcgcgcgt ccccgtcatc aacgccggcg acggcaccaa ccagcacccc
     3481 agccagacct tgttggatct ggtaacgatt tatgaaacac agggacgttt ggacaagctc
     3541 aaaatcgcca tggcgggcga cttgaaatac ggacgtaccg tgcattcgct ttgtcaggcg
     3601 ttgaaacgct ggaattgtga atttgccttt gtttcgccgc ccagcctagc catgcccgac
     3661 tatattaccg aagagttgga cgaagccggc tgccgatacc gtatcctcgg tagtttggaa
     3721 gaagcggcgg aatgggcgga tatcctgtat atgacccgcg tccagcgcga acgtttcgac
     3781 gaacaggaat ttgccaaaat ccaaggcaaa ttcaacctcg aagcgtctat gctcgcccgc
     3841 gccaaaccga acctgcgcgt gctgcacccc ctgccgcgcg tggacgaaat ccatcccgat
     3901 gtcgatgcca cgccgcacgc ctattatttc gagcaggcga ccaacggcgt ttatgcgcgt
     3961 atggcgatat tgtcgctggt gttgaacgaa gaagtgtgag gaaccgatat ggaaaccccg
     4021 aaactcagtg tcgaagccat tgaaaaaggt acggttatcg accatattcc cgccggcagg
     4081 gggctgacca tcctgcgcca gttcaaactt ttgcactacg gcaacgcggt aaccgtgggc
     4141 ttcaacctgc ccagcaaaac ccaaggcagc aaagacatca tcaaaatcaa aggcgtgtgc
     4201 ttggacgaca aagccgccga ccgcctcgcc ctgtttgcac ccgaagcagt ggtcaacacc
     4261 atcgaccatt tcaaggtcgt gcagaaacgg catttgaacc tgcccgacga aatcgccgaa
     4321 gtgttccgct gtccgaacac gaattgcgcc ggccacggcg agccggtcaa aagccggttt
     4381 tatgttaaaa aacacaacgg gcagacgcgg ctgaaatgcc actactgcga aaaaacctac
     4441 agccgggatt cggtggcgga agcctgacgg attcccttaa accgagtggg cggcatttcg
     4501 tctgccgcct gttttgccaa tctgaaatgg aatgatgatg cacgcttctg tccaaagccg
     4561 tttcgcaccg atactttatg ttttgatttt ctttgccggt tttttgaccg cgcaaatctg
     4621 gttcaatcag aaagcctata ctgaagagct gcctccgctt ctgtccgcat tgtccgctgt
     4681 cgcgctggtg tggctggcgt gggcgttcgt gtcggcgcgt tcaaaggcta aggcggaaaa
     4741 gttctaccgc gaaaaaatga tacagaacga aagcatacac cccgtcctgc acgcttcttt
     4801 gcaacacttg gaacacaagc cgcaaatgct cgccctgctg gtcaaaaacc acggcaaagg
     4861 gatggcggaa caggtcaggt tcaaggcgga agtgctgccc gacgacgaag acgcgcgcac
     4921 gattgccgcc gagttggcaa aaatggatat gttcgcattg gggacggacg cggtcgcctc
     4981 gggcgaaacc tatggacgcg tgttcgccga tattttcgag ttgtcggcgg ctttggaagg
     5041 gcgcgcgttc aaaggaatgt tgaaactgac ggcggaatat aaaaacatct tcggcgatgc
     5101 ctgccgttcg gaaacggcgt tggagttggg cgcgctcaat caggcgttgc aggagatttc
     5161 aaaaacatcg gaaaagtcca aacggatatt ttattgaaga tggaaaaatg ccgtctgaaa
     5221 cggaaggtgt ttcagacggc atttttgtcg gatgattaat tattcggagc ggttgaagcc
     5281 aaacttcacg cggctgcggc cctgatccgg tatattgtcc aaatcgcgtc ccggattggc
     5341 ggcggtgtcg cctacggaaa tatcggagat gttttccaaa atgatggcgg acgacaggtg
     5401 ttcggaggtg cggtaaacca ttgccaagcc cacttcttcg gcaggagtgg aaatcagctc
     5461 gacggtatcc ctgcttttga aattgttgga gaggtcgacc tgcatcgttt tcttgcgttt
     5521 gtagaggctc aaaaccgtgc ctttgtccaa accgtccgcc tcgcctttgt cgatggtgat
     5581 ggtttgaaac tggccggcaa tccttgtgcc ttcaaacacg gaaacgattt tagcctgaac
     5641 cgggcgggac ggttcgtgcg gcatcatgtt gaagcggtcg gtgtcttccg gcattttcat
     5701 caggtagtcg ccctgctgta tttcggaaat ggcggtttcg accaccagcg gctgtatcga
     5761 aggggtgcgc agcggggtaa tcaaaggatg ggtgcgggta tggtattcgt tgtctttcgg
     5821 ccgttctcca gcctgtttcg agcgttgttc gaggacagag tcggtatagt cgagggagcg
     5881 cacgatgccg ctgaatgcga cttcctgccc gaggaattta cccgtatccg gatcggtgat
     5941 gtttttattg attcggtagg tcaggtagcg gcccggctct ttcaggcctt tggtgtaaac
     6001 cctggtgcct ttggtgtaca gcagcctgcc ttccgggccc gagagcaggc gcggcgcggc
     6061 agcggtttct ttgcgggaaa cgatttgcgg atgccgcata aagatgcggt agaagttgac
     6121 atcgatggcg ggaataccgt atccggacac ttccttatcc ggactcattt tgacgacggg
     6181 gatgccgtct gtctgttcca agccgaggcg cggttcgccg tcaacgtggc gcaacaccaa
     6241 tacctggtcc ggataaatca ggtcgggatt gtggatttga tcccggttcg cgtcccacag
     6301 gcggccccat tgccacgggc tgtacaggta tttgcccgaa atgccccaca gggtgtcgcc
     6361 ctgtttgacc gtgtagcgtt ccggcgcgtt cgggcgcacc tccaaatttg ccgccaaagt
     6421 ttgtgttgag aatgccatac ctgccgcgca gagcagggtt ataatacgac gttgcataac
     6481 cgttcccctt atctgataaa tttcggtttg tcttgcttga ttgggttgga aaaagcggcg
     6541 gcagcccctc gggatgtgcc gcgtgataaa aaatgttccg cattttaaca tcgaattatc
     6601 cgcaccatca cggtaattat gaaaaacagg cggcgtatcc gccgaaggaa agagaaaatt
     6661 atggctttat tgaatatctt gcaatatccc gacgagcgtc tgcacacggt ggcaaagcct
     6721 gtcgaacaag tcgacgagcg catccggaag ctgattgccg atatgtttga aacgatgtac
     6781 gaatcgcgcg gcatcgggct ggcggcgacg caggtcgatg tgcacgagcg cgtggtcgtg
     6841 atggatttga ccgaagaccg cagcgaaccg cgcgtgttca tcaaccccgt catcgttgaa
     6901 aaagacggcg aaaccactta cgaagagggc tgcctgtccg tgccgggcat ttacgacacc
     6961 gtaacccgcg ccgaacgcgt caaggtcgag gctttgaacg aaaaaggcga aaagttcacg
     7021 ctggaggcgg acggcttgtt ggcgatttgc gtgcagcacg agttggacca cctgatgggc
     7081 atcgtgtttg tcgaacgcct ttcccaactc aagcaggggc ggattaagac caagctgaaa
     7141 aaacgtcaga aacatacgat ttgacccttt tgccgtgccg tctgaacgct gcaaagtttt
     7201 cagacggcac ggtcttgtcc gacaatttta cgcacgcgca ggaacacgct atgaaagtca
     7261 tcttcgccgg cacgcccgat tttgccgccg ccgccttaag agccgttgcc gccgccggtt
     7321 ttgaaattcc gctggtgctg acccagcccg accgtccgaa agggcgcggt atgcaactga
     7381 ctgccccgcc cgtcaaacaa gccgcgctgg aactcggttt gcgcgtcgaa cagcccgaaa
     7441 agctgcgcaa caacgccgaa gccctgcaaa tgctcaaaga ggtcgaggca gacgtaatgg
     7501 tggttgccgc ctacggtttg attctgccgc aggaagtgtt ggatacgccg aaacacggct
     7561 gcctcaacat ccacgcttcg ctgttacccc gttggcgtgg cgcggcgccg attcaacgcg
     7621 cgattgaagc cggcgatgcc gagacaggcg tgtgtattat gcagatggac atcggtttgg
     7681 acaccggcga tgtggtcagc gaacaccgct acgccatcca accgaccgat accgccaacg
     7741 aagtccacga cgcgctgatg gaaatcggtg cggcggcggt tgttgccgat ttgcaacagc
     7801 ttcaaagcaa aggccgtctg aacgcggtca aacagcccga agaaggtgtt acttacgcgc
     7861 aaaaattgag caaagaagag gcgcgtatcg attggagcaa aagcgcggcg gttatcgaac
     7921 gcaaaatccg cgccttcaac cccgtgcctg ccgcgtgggt tgagtatcag ggcaagccga
     7981 tgaaaatccg gcgggcggaa gtggtggcgc aacaaggcgc ggcaggcgaa gtgttgtcct
     8041 gttcggcgga cggtttggtc gttgcctgcg gcgaaaacgc gctgaagatt accgaattgc
     8101 agcctgccgg cggcaggcgg atgaatatcg cggcgtttgc agcaggacgg catatcgaag
     8161 caggggcgaa gctgtaaatc ccttcagacg gcattccgat ccgcaaacgg gaatgccgtc
     8221 tgaaaccatc agtcgaagaa agcgaatcac ataatatgag tatggcactt gcccaaaaac
     8281 ttgccgccga cagcattgcg gcggttgccg aaggacgtaa ccttcaggac gtgttggcgc
     8341 aaatccgcac cgcgcatccc gaccttatgg cgcaggaaaa cggcgcgttg caggacatcg
     8401 cctacggctg ccagcgttat ttgggcagtt tgaaacatat gctcgcgcag atgctgaaaa
     8461 agccgattgg caatccgcag ctcgaaagcc tgcttttggc ggcgttgtac cagctgcatt
     8521 acacgcgcaa cgcgccccac gccgtggtca atgaggcggt ggaaagcatc gcgaaaatcg
     8581 gacgcgggca gtaccgttcg tttgccaacg cggttttgcg ccgctttttg cgcgaacgcg
     8641 acaagcttgt ggcttcctgt aaaaaagacg atgtagcgaa acacaacctg ccgctgtggt
     8701 gggtggctta cttgaaaaac cattatccga aacactggca caacatcgcc gccgcgctgc
     8761 aatcccatcc gccgatgact ttgcgcgtca accgccgaca cggcaatgcc gaaagctatt
     8821 tggaaaaact ggtggcggaa ggtatcgcgg ctaaggcgtt ggacgaatat gcggttacgt
     8881 tggaagaagc cgtgccggtg aaccgcctgc ctggtttttc agacggcatt gtttcggtac
     8941 aggacttcgg cgcgcagcag gcggcgtatt tgttaaaccc gaaagacggc gaacggattt
     9001 tggacgcgtg cgccgcgccg ggcggcaaga cggggcatat cttggaactg gcggattgcc
     9061 gtgttaccgc cttggacatt gatgcaggcc gtctgaaacg ggtggaagac aatatcgcgc
     9121 gtctgggctt tcagacggca tcgacggcgt gtgccgatgc acaggacctg tcggcatggt
     9181 atgatgggaa accgtttgat gccgtccttg ccgacgtgcc gtgtaccgcc tcgggcgtgg
     9241 cgcggcgcaa tcccgacgtg aaatggctac gccgtccgac cgacgcgctc aaaaccgccc
     9301 gccagcagga agccctgcta gatgcattgt ggcaggtgct gaaaagcggg ggaaggatgt
     9361 tgatcgctac ctgttccgtg ttcgtcgagg aaaacgacgg acaattgcaa aaattcctca
     9421 accgccatgc cgatgcagaa ctgatcgaat cgcgggtact cttaccgaac aaacaccaag
     9481 atggctttta ttacgcgctt attcaaaagc agtaaatggc tgattgtgcc gctgatgctc
     9541 cccgcctttc agaatgtggc ggcggagggg atagatgtga gccgtgccga agcgaggata
     9601 accgacggcg ggcagctttc catcagcagc cgcttccaaa ccgagctgcc cgaccagctc
     9661 caacaggcgt tgcgccgggg cgtgccgctc aactttacct taagctggca gctttccgcc
     9721 ccgataatcg cttcttatcg gtttaaattg gggcaactga ttggcgatga cgacaatatt
     9781 gactacaaac tgagtttcca tccgctgacc aaccgctacc gcgttaccgt cggcgcgttt
     9841 tcgacagact acgacacctt ggatgcggca ttgcgcgcga ccggcgcggt tgccaactgg
     9901 aaagtcctga acaaaggcgc gctgtccggt gtggaagcag gggaaaccaa ggcggaaatc
     9961 cgcctgacgc tgtccacttc aaaactgccc aagccttttc aaatcaatgc attgacttct
    10021 caaaactggc atttggattc gggttggaaa cctctaaaca tcatcgggaa caaataatgc
    10081 gccgttttct accgatcgca gccatatgcg ccgtcgtcct gttgtacgga ctgacggcgg
    10141 caaccggcag caccagttcg ctggcggatt atttctggtg gattgttgcg ttcagcgcaa
    10201 tgctgctgct ggtgttgtcc gccgttttgg cacgttatgt catattgctg ttgaaagaca
    10261 ggcgcgacgg cgtattcggt tcgcagattg ccaaacgcct ttctgggatg tttacgctgg
    10321 ttgccgtact gcccggcgtg tttctgttcg gcgtttccgc acagttcatc aacggcacga
    10381 ttaattcgtg gttcggcaac gatacccacg aggcgcttga acgcagcctc aatttgagca
    10441 agtccgcatt gaatttggcg gcagacaacg ccctcggcaa cgccgtcccc gtgcagatag
    10501 acctcatcgg cgcggcttcc ctgcccgggg atatgggcag ggtgctggaa cattacgccg
    10561 gcagcggttt tgcccagctt gccctgtaca atgccgcaag cggcaaaatc gaaaaaagca
    10621 tcaacccgca caagctcgat cagccgtttc caggtaaggc gcgttgggaa aaaatccaac
    10681 gggcgggttc ggtcagggat ttggaaagca taggcggcgt attgtacgcg cagggctggc
    10741 tgtcggcggg tacgcacaac gggcgcgatt acgccttgtt tttccgtcag ccggttccca
    10801 aaggcgtggc agaggatgcc gtcttaatcg aaaaggcaag ggcgaaatat gctgagttga
    10861 gttacagcaa aaaaggtttg cagacctttt tcctggcaac cctgctgatt gcctcgctgc
    10921 tgtcgatttt tcttgcactg gtcatggcac tgtatttcgc ccgccgtttc gtcgaacccg
    10981 tcctatcgct tgccgagggg gcgaaggcgg tggcgcaagg cgatttcagc cagacgcgcc
    11041 ccgtgttgcg caacgacgag ttcggacgct tgaccaagtt gttcaaccac atgaccgagc
    11101 agctttccat cgccaaagaa gcagacgagc gcaaccgccg gcgcgaggaa gccgccaggc
    11161 attatcttga atgcgtgttg gaggggctga ccacgggcgt ggtggtgttt gacgaacaag
    11221 gctgtctgaa aaccttcaac aaagcggcgg aacagatttt ggggatgccg cttacccccc
    11281 tgtggggcag cagccggcac ggttggcacg gcgtttcggc gcagcagtcc ctgcttgccg
    11341 aagtgtttgc cgccatcggc gcggcggcag gtacggacaa accggtccat gtgaaatatg
    11401 ccgcgccgga cgatgccaaa atcctgctgg gcaaggcaac cgtcctgccc gaagacaacg
    11461 gcaacggcgt ggtaatggtg attgacgaca tcaccgtttt gatacacgcg caaaaagaag
    11521 ccgcgtgggg cgaagtggcg aagcggctgg cacacgaaat ccgcaatccg ctcacgccca
    11581 tccagctttc cgccgaacgg ctggcgtgga aattgggcgg gaagctggat gagcaggatg
    11641 cgcaaatcct gacgcgttcg accgacacca tcgtcaaaca ggtggcggca ttgaaggaaa
    11701 tggtcgaagc attccgcaat tatgcgcgtt ccccttcgct caaattggaa aatcaggatt
    11761 tgaacgcctt aatcggcgat gtgttggcat tgtatgaagc cggtccgtgc cggtttgcgg
    11821 cggagcttgc cggcgaaccg ctgacggtgg cggcggatac gaccgccatg cggcaggtgc
    11881 tgcacaatat tttcaaaaat gccgccgaag cggcggaaga agccgatgtg cccgaagtca
    11941 gggtaaaatc ggaaacaggg caggacggtc ggattgtcct gacggtttgc gacaacggca
    12001 aagggttcgg cagggaaatg ctgcacaacg ccttcgagcc gtatgtaacg gacaaaccgg
    12061 cgggaacggg attgggtctg cctgtggtga aaaaaatcat tgaagaacac ggcggccgca
    12121 tcagcctgag caatcaggat gcgggtggcg cgtgtgtcag aatcatcttg ccaaaaacgg
    12181 taaaaactta tgcgtagcag cgatatttta attgtagacg acgaaatcgg catccgcgac
    12241 ctgctgtcgg aaatcctgca ggacgaaggt tattcggtcg cattggcgga aaacgccgaa
    12301 gaggcgcgca agctgcgcca tcaggcgcgc cccgcgatgg tgctgctgga tatttggatg
    12361 cctgattgcg acggcatcac ccttttgaag gagtgggcga aaaacgggca gctcaatatg
    12421 ccggtggtga tgatgagcgg gcatgccagc atcgataccg ccgtggaagc caccaaaatc
    12481 ggcgcgatcg attttttgga aaaaccgatt tccctgcaaa agctgctgtc tgccgtcgaa
    12541 aacgcgttga agtacggtgc ggcgcaaacc gaaacggggc ctgtattcga caagctgggc
    12601 aacagtgcgg cgattcagga aatgaaccgt gaggtagggg ctgcggtgaa atgtgcctct
    12661 cccgtacttt tgacgggcga ggcgggttcg ccgtttgaaa cggtggcacg ctatttccat
    12721 aaaaacggta cgccgtgggt cagcccggca agggtcgaat atctgatcga tatgccgatg
    12781 gaactgttgc agaaggcgga gggcggcgtt ttgtatgtcg gcgacatcgc ccagtacagc
    12841 cgcaacatcc aagccggtat tgcctttatt gtcggaaagg cggaacaccg ccgcgtcagg
    12901 gtggtcgcat cgggcagcag ggcggcaggt tcagacggca ttgcctgcga ggaaaagctg
    12961 gcggaactgc tgtcggaatc ggtcgtccgt attccgccgc tgcgtatgca gcatgaagac
    13021 attcccttcc tgatacaggg gattgcctgc aatgtggcgg aaagccaaaa gattgcgcct
    13081 gcctcattca gtgaagaggc acttgccgca ttgacccgtt acgactggcc gggaaatttc
    13141 gaccaactgc aaagcgtcgt tgcaacgctg ttgttggagg cggacggaca ggaaatcggc
    13201 gcaggggcgg tttcttccct tttggggcag aatgtgcctg ccgagggggc ggaagatatg
    13261 gtgggcgggt ttaatttcaa cctgcccctg cgcgaattga gggaggaggt ggagcggcgt
    13321 tatttcgagt accacatcgc ccaagaaggt cagaatatga gccaagtggc gcagaaagtt
    13381 ggtttggaac gcacgcacct ttaccgcaaa ctcaaacagc tcggcatcgg cgtttcgcgc
    13441 cgggcggggg aaaaaaccga agaataggcc cggacggccg gtttaccggc tgcgggcttt
    13501 tgttttcaga cggcatttgg tgcaaatgcc gtctgaaatc gtaaggggac ggattttatg
    13561 acagaggacg aacgtttcgc gtggctgcaa ttggcgttta cgccctatat cggcgcggaa
    13621 agtttcctgc tgctgatgcg ccgtttcggc agcgcgcaaa atgccctgtc cgcaccggcg
    13681 gaacaggtgg cggcactgat acggcacaaa caggcgcttg aggcttggcg caatgcggaa
    13741 aaacgcgctc tggcgcggca ggcggcagaa gcggcattgg aatgggaaat gcgggacgga
    13801 tgccgcctga tgctgcttca ggatgaagat tttcccgaaa tgctgacgca ggggctgacc
    13861 gcgccaccgg ttttgttttt gcgcggcaac gtgcaactgc tgcacaaacc ttccgccgcc
    13921 atcgtcggca gccgtcatgc cacgccgcag gcgatgcgga ttgccaaaga tttcggcaag
    13981 tcgttgggtg ggaaaggcat tcccgttgtg tcgggtatgg cttcgggcat cgataccgcc
    14041 gcccatcagg gtgcgttgca ggcagaaggc ggcaccatcg ccgtgtgggg gacgggcata
    14101 gaccgcattt atccgccgtc caataaaaac cttgcctatg aaatcgccga aaaagggctg
    14161 attgtcagcg agttccccat cggcacgcgg ccgtatgccg gcaattttcc gcgccgcaac
    14221 cgcctgattg ccgccctgtc gcaggttacg ctggtggttg aagccgcgtt ggaatccggt
    14281 tcgctgatta ccgccggcct ggcggcagaa atggggcgcg aagtgatggc ggtacccggc
    14341 tcgatagaca atccgcacag caaaggctgc cacaaactga ttaaagacgg cgcgaaactg
    14401 acggaatgtt tggacgacat cctgaacgaa tgcccggggc tattgcaaaa tacgggtgct
    14461 tcatcatatt ctataaataa ggatacgccc gacacgggac gccgcgccgt tcagacggca
    14521 tacgccccgc cgcctgccgc aaaaatgccg tctgaagggg cggcaggcgg cacagccgtc
    14581 ggcggcatac tggataaaat gggtttcgac cccatccatc ccgacgtgct tgccggacag
    14641 ttggctatgc ctgccgcaga tttgtatgcc gcactgttgg aattggaatt ggacggcagc
    14701 gttgccgcaa tgcccggcgg cagataccag cgtatccgaa cttgaacgca ctttatatta
    14761 aggaacacga atgaccgaag tcatcgccta cctcatcgaa catttccaag atttcgatac
    14821 ctgtccgccg cccgaagact tgggtatgct gcttgaagaa gcgggtttcg atacgatgga
    14881 aatcggcaac accctgatga tgatggaagt attgctcaac agctccgaat tttccgccga
    14941 acctgccgac agcggcgcat tgcgcgtgta cagcaaagaa gaaaccgaca acctgccgca
    15001 ggaagtgatg gggctgatgc agtatctgat tgaagaaaaa gccgtcagct gcgaacagcg
    15061 ggaaatcatc atccacgcgc tgatgcacat tccgggcgac gaaattaccg tagataccgc
    15121 caaagtgctg accctgctgc ttttatgggc aaacaagagc gagctgcccg tgttggtcgg
    15181 cgacgagctg atgagcgcgc ttttactcga caacaaaccc acgatgaact gaagcggctt
    15241 cagacggcat tatcctgcta acctgccgtc tgaaacgccg gcatcaaaac caccatccag
    15301 agaacgacaa atggcgaaaa acctattaat cgtcgaatcc ccgtccaaag ccaaaaccct
    15361 gaaaaaatat ttgggcggcg attttgaaat ccttgcatcc tacggacacg tccgcgacct
    15421 cgtccccaaa agcggcgcgg tcgatcccga caacggcttt gcgatgaaat accaactcat
    15481 cagccgcaac ggcaaacacg tcgatgccat cgtcgccggt gccaaagaag ctgaaaacat
    15541 ctacctcgcc accgacccgg atagggaagg cgaagccatt tcctggcatc ttttggaaat
    15601 cctcaaatcc aaacgcggct tgaaaaacat caagccgcag cgtgtcgtgt tccacgaaat
    15661 caccaaaaac gccgtgctcg atgccgttgc ccatccgcgc gaaatcgaaa tggacttggt
    15721 ggatgcacaa caagcccgtc gcgctttgga ctatttggtc ggctttaacc tttcgccatt
    15781 gttatggaaa aaaatccgcc gcggtttgag cgcaggccgc gtgcaaagcc ccgctttgcg
    15841 cctgatttgt gagcgcgaaa acgaaatccg cgcgtttgaa gcgcaggaat attggacggt
    15901 gcatctcgac agccacaaag gccgcagcaa gttcaccgcc aaactcgccc aatacaacgg
    15961 cgcgaaactc gaacaattcg acctgccgaa cgaagccgcg caagccgacg tgttgaaaga
    16021 actcgaaggc aaagaggccg tcgttaccgc cattgaaaag aaaaagcgca gccgcaatcc
    16081 tgccgcgccg ttcaccactt ctaccatgca gcaggatgcc gtgcgcaaac tcggcttcac
    16141 caccgaccgc accatgcgta ccgcccaaca gctttatgaa ggtatagacg tagggcaggg
    16201 cgcgatcggt ctgattacct atatgcgtac tgacagcgtg aatttggcgg atgaagcgtt
    16261 aaccgaaatc cgccattaca tcgaaaacaa aatcggcaaa gaatatctgc cgagtgccgc
    16321 caaacaatac aaaaccaaat ccaaaaacgc ccaagaagcg cacgaagcca tccgtccgac
    16381 ttccgtgtac cgcacgcccg aaagcgtcaa acccttcctg agcgcagacc agttcaaact
    16441 ctatcaaatg atttggcagc gtaccgtcgc ctgtcagatg acgcctgcca aattcgacca
    16501 aaccaccgtc gatattaccg tcggcaaagg cgtattccgc gtaaccggac aagtgcaaac
    16561 cttcgcaggc ttcttgagcg tttatgaaga aagcagcgac gatgaagaag gcgaagacag
    16621 caaaaaactg cccgaaatga gcgaaggcga taaattgccc gtggacaaac tctacggcga
    16681 acaacacttt accactccgc cgccgcgcta caacgaagcc acgctggtta aagccctcga
    16741 agaatacggc atcggccgcc cctcgaccta cgccagcatc atctccacgc tcaaagaccg
    16801 cgaatacgtt acccttgagc aaaaacgctt catgcccacc gacacaggcg acatcgtcaa
    16861 caaattcctg accgaacact tcgcccaata cgtcgattac cacttcaccg ccaaactcga
    16921 agaccagctt gacgaaattg ccgacggcaa acgccaatgg attcccttga tggacaaatt
    16981 ctggaaaccg ttcatcaaac aagtggaaga aaaagaaggc atcgaacgcg ccaaatttac
    17041 cacgcaggaa cttgatgaaa cctgcccgaa atgcggcgaa cacaaactgc aaatcaaatt
    17101 cggcaaaatg ggtcgttttg ttgcgtgtgc cggttatccc gagtgcagct acacgcgcaa
    17161 tgtcaacgaa accgccgaag aggccgccga gcgcatcgcc aaagccgaag ccgaacaggc
    17221 cgaactcgac ggacgcgagt gcccgaaatg tggcggtcgc ctagtgtaca aatacagccg
    17281 caccggcagc aaattcatcg gctgcgccaa ctatccgaaa tgcaaacacg tcgagccgct
    17341 ggaaaaaccg aaagataccg gcgtccagtg tccgcaatgc aaaaaaggca acctcgtcga
    17401 gcgcaaatcc cgctacggca aactgtttta cagttgcagc acctatcccg actgcaacta
    17461 cgccacttgg aacccgcccg ttgccgaaga atgcccgaac tgccattggc cggtcttgac
    17521 catcaaaacc accaaacgct ggggcgtcga aaaagtctgc ccgcaaaaag aatgcggctg
    17581 gaaagaacag attgaaccgc ccgcgccgaa ggagtaagat taggttggtt tgaaagagaa
    17641 aaggtcgtct gaaaaatttt cagacgacct ttgcttttct gtgattggtt tatttgaatc
    17701 cgcgtgttgt tttaaagtcc gataaaatcc ggttcatttc aggcacaaac aaggcgatgt
    17761 aatcgtaaga tagaccgcga ctggcactgg gatggggaaa gcagacgact tcgcaatctt
    17821 caaacgattg gaatttgaca ttgaaacgtg taccgtcaaa ttctttttgc accgtctcca
    17881 gcggtttggt ctgcttaccg accaactgct cgaagcgtgg cagtacattt tggttgttca
    17941 gaaaatccgc caacctgctg cccatgaaga ggatgacttt cggacgcagt ttttcgatgt
    18001 ggtagagaaa attatcgatg tgctcgggtt gtgtgaactt gtcgggattg tcgatagtgt
    18061 tgccctgtgt agcagcccag ttggtttgaa ccagggattt ttcaaatgca ccgcccaatc
    18121 cattttcgtc taaggggtgt ccccacattt caaaccaatt ttttatcgta ttgtcgtaac
    18181 gccacttttt tgcctgctct ccgaaataga gggatttgtt tgcaaatgta tggtcgattt
    18241 tgttttcagg gagtttgtat tcacctgcta cataagcagc ctcatcggct ttactccaac
    18301 cccattcata gccacaaatc attaagccat gtttgtcgtt gtagcctttg aacaggctgt
    18361 tgctcaaatt caaatccttc atcatgaact cttcctttta aaatttaaga gcgattgact
    18421 tcaatgtttt tagatggggt ggaaaaatcc ttgtgtaggc aacataaatt caataaattt
    18481 cttgataatt cgaaacctac taatagcgca cctataaaag ctttttcatt acgttcagca
    18541 tgacggtcac gtcgttcata ttttttacgc ttgctgttcc ctgttattac agctaagcca
    18601 agtgatatgg cgagaattgc ccaaacaata gtacttaata acaattttcc ccatactatc
    18661 aatagggaaa gaaaaaacct tttagtatta ggtcgataga ttataatcca tgcccatgaa
    18721 aatgcaataa gagttataca taagatacaa gctgatagga tttctttatt tttaattaaa
    18781 taacttaggg cgatgaggat gacaggtgtc aagaaaacaa tagttacatc ccgatagcta
    18841 taaaagaaaa ctgccccatt ttgaatgtgg aaatgtgcac agaatccaat atagctaagg
    18901 ataatagtta atataataaa aaaagaccac caagggtgaa gagataggaa ttccatgttt
    18961 tccctttttt tgtaaaaagg aaaaaatctt atctaatagt taattgccta accagccaga
    19021 agaagtttaa aatctatccc aataattcaa ccatctatac agaaagttca gcttatggaa
    19081 acccacgaaa aaatccgcct gatgcgcgaa ttgaataaat ggtcccagga ggatatggcg
    19141 gaaaagctgg cgatgtcggc aggcgggtat gccaaaatcg aacgaggcga aacgcagttg
    19201 aatatcccgc gtttggagca gttggctcag attttcaaaa tcgatatgtg ggacttgctc
    19261 aaatcgggcg gtggtgggat ggtgtttcag attaatgaag gtgatagtgg tggcgatatt
    19321 gcgttgtatg cgtcgggtga tgtttcgatg aaaatagaat ttttaaaaat ggagttgaaa
    19381 cactgcaaag aaatgttgga acaaaaagac aaagaaatcg agctgctccg caagctgacc
    19441 gaaaccgttt aaacagatat gccgtctgaa aaaagttttc agacggcata ttctttgaca
    19501 ggtcttgtat aataccgttt gaacttacag gtttttgatt atggcggcag gcaaacatac
    19561 caaacacagc aaccgggtac gcattatcgg cgggcaatgc cggggcagga aattgagttt
    19621 cacatccgcc gacggactgc gtccgacacc cgacagcgtg cgtgaaaagc tgtttaactg
    19681 gctgggacag gatttgacgg gtaaaacggt tttggatctc ttcggaggca gcggcgcact
    19741 cggtatagaa gccgcttcgc gcaacgccaa acgcgtgctg atttcggata acaaccgcca
    19801 aaccgtgcag accttgcaga aaaacagtcg cgaactgggt ttggggcagg tgcaaatcgt
    19861 cttttcagac ggcatcgcat atttgaagac cgtatccgaa cagtttgatg ttgtctttct
    19921 cgacccgccg tttgcatggc aggactggca aatcctgttc gatgccttga agccgtgcct
    19981 gaacccccgg gcattcgtct atctcgaggc gggtacgctg ccgaatattc ccgattggct
    20041 gacggaatat agagaaggga aatcggggca gagtacattc gaattaaggg ttttccaagt
    20101 ggctgaataa tatgcgcttt gataatcatt tccgagttgt aaacattcgt ttgcaaccgt
    20161 ccggttcaaa aaaaccttgt gctataatcc gcgcccgccc ggttttgata atttagtgga
    20221 aaaggaaaag aaatgtcgct ttttattacc gacgagtgca tcaactgcga cgtatgcgaa
    20281 cccgaatgcc ccaatgatgc catttcccaa ggcgaggaaa tttacgaaat caaccccaac
    20341 ctctgcacgc agtgcgtcgg acactacgat gagccgcagt gccagcaggt ttgcccggtg
    20401 gactgcatcc tgattgacga agaacatccc gaaacccatg acgagttgat ggcgaaatac
    20461 gaaaagatta tccagtttaa ataaattctt tttaaaacat caaattatgt ctgttttgaa
    20521 ataaaatcaa aaaaaaactt gacggaaaag caagccgcta ataaactaac gttctctttt
    20581 ggagggattc ccgagcggtc aaagggggca gactgtaaat ctgttgcgaa agcttcgaag
    20641 gttcgaatcc ttctccctcc accaaaattc ttacttgggg cagtagcgag taatgcgggt
    20701 gtagctcaat ggtagagcag aagccttcca agcttacggt gagggttcga ttcccttcac
    20761 ccgctccaaa caattaggcc catgtagctc aggggtagag cactcccttg gtaagggaga
    20821 ggtcggcagt tcaaatctgc ccatgggcac catccctcga ttattcattt ctttaaggct
    20881 tagatatata ggatattgcc atggctaagg aaaaattcga acgtagcaaa ccgcacgtaa
    20941 acgttggcac catcggtcac gttgaccatg gtaaaaccac cctgactgcc gctttgacta
    21001 ctattttggc taaaaaattc ggtggtgctg caaaagctta cgaccaaatc gacaacgcac
    21061 ccgaagaaaa agcacgcggt attaccatta acacctcgca cgtggaatac gaaaccgaaa
    21121 cccg
//
LOCUS       DAWIVB010000016        20711 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWIVB010000016 DAWIVB010000000
VERSION     DAWIVB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.016"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(63..239)
                     /locus_tag="WIX60_000418"
     CDS             complement(63..239)
                     /locus_tag="WIX60_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304737.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1609)
                     /locus_tag="WIX60_000419"
     CDS             complement(242..1609)
                     /locus_tag="WIX60_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ5304738.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GERFDAHDFVEDVLAAGAAAVVVSREDCAAMDGALKVDDTLAALQTLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPNAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     IALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGELGEDEAAAMHAEVGAYARDQGIE
                     AAYFVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVE
                     ALEDK"
     gene            complement(1734..3380)
                     /locus_tag="WIX60_000420"
     CDS             complement(1734..3380)
                     /locus_tag="WIX60_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ5304739.1"
                     /translation="MKQSARIKNMNQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3403..4881)
                     /locus_tag="WIX60_000421"
     CDS             complement(3403..4881)
                     /locus_tag="WIX60_000421"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ5304740.1"
                     /translation="MFSKLTPLAETGIPPLSCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAIANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGMLAAEVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQATDLLGEKTAIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGGIKSRLFYWHGLKHAVINTDDEYGAELVCRLKKDCSDLTVYSYGFSEQADIRITDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPAPECVEADRAAAIRYAVE
                     RAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4906..6654)
                     /locus_tag="WIX60_000422"
     CDS             complement(4906..6654)
                     /locus_tag="WIX60_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ5304741.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGHADSEQRRNRAVTDMIEPGSAIKPFVIAKALDADKTNLNERLNT
                     QPYKIGPAQVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFSSKEMYDLYHELGIGVRM
                     HSGFPGESAGALRNWQKWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKASTARQVRELMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNGR
                     YVDYKHVATFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVTGPVFKQVMGGSLNILGV
                     SPTKPLTNVAAVKTPS"
     gene            complement(6715..6978)
                     /gene="ftsL"
                     /locus_tag="WIX60_000423"
     CDS             complement(6715..6978)
                     /gene="ftsL"
                     /locus_tag="WIX60_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ5304742.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(6975..7961)
                     /gene="rsmH"
                     /locus_tag="WIX60_000424"
     CDS             complement(6975..7961)
                     /gene="rsmH"
                     /locus_tag="WIX60_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ5304743.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRRIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7958..8413)
                     /gene="mraZ"
                     /locus_tag="WIX60_000425"
     CDS             complement(7958..8413)
                     /gene="mraZ"
                     /locus_tag="WIX60_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ5304744.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVSAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8692..9903
                     /locus_tag="WIX60_000426"
     CDS             8692..9903
                     /locus_tag="WIX60_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ5304745.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPSPEARQFSLKLQTLIAE
                     KIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQL
                     QELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSA
                     RPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHIIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYICEAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKL"
     gene            10035..10111
                     /locus_tag="WIX60_000427"
     tRNA            10035..10111
                     /locus_tag="WIX60_000427"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10069..10071,aa:Met,seq:cat)
     gene            10119..10194
                     /locus_tag="WIX60_000428"
     tRNA            10119..10194
                     /locus_tag="WIX60_000428"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10152..10154,aa:Ala,seq:ggc)
     gene            complement(10476..11297)
                     /locus_tag="WIX60_000429"
     CDS             complement(10476..11297)
                     /locus_tag="WIX60_000429"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ5304746.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11330..11974)
                     /locus_tag="WIX60_000430"
     CDS             complement(11330..11974)
                     /locus_tag="WIX60_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ5304747.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11977..12840)
                     /gene="ftsN"
                     /locus_tag="WIX60_000431"
     CDS             complement(11977..12840)
                     /gene="ftsN"
                     /locus_tag="WIX60_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ5304748.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMG
                     AYADRQSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12946..14442)
                     /locus_tag="WIX60_000432"
     CDS             complement(12946..14442)
                     /locus_tag="WIX60_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ5304749.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDMEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLE
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14456..14776)
                     /locus_tag="WIX60_000433"
     CDS             complement(14456..14776)
                     /locus_tag="WIX60_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ5304750.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLEKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15259..16785
                     /gene="putP"
                     /locus_tag="WIX60_000434"
     CDS             15259..16785
                     /gene="putP"
                     /locus_tag="WIX60_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ5304751.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRATR
                     "
     gene            17035..20640
                     /gene="putA"
                     /locus_tag="WIX60_000435"
     CDS             17035..20640
                     /gene="putA"
                     /locus_tag="WIX60_000435"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ5304752.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRTQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4886 a         5955 c         5010 g         4860 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaattgggt aactccccgg atttttaccg
      121 ccgcccaagt ctaaacataa atcttcataa agatacgcct gcgtccgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcagcaat aaggctttaa aaaaacgtcg gcttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gggcatacgc gccgacttcg gcgtgcatag
      481 cggcggcttc gtcctcgccc agttcgccca gttcgcccat atcgcccatc acgaaaatac
      541 gcggcgcagg catacgcgcc aacacgtcaa tcgcagcttt catgctgtca gggttcgcat
      601 tataagtatc gtcaatcagg gttgcgccct tgattccgga tttgacgttc agacggcctt
      661 tgatattgct gaagcctttc aaaccttccg ccacatcgtt caaactcaaa cccgcagcca
      721 aagccagcgc ggcggcagcg gcggcgttgt ggacattgtg gcggccggga acaggcagca
      781 ccacggcggc gcgctcatcg ccgcacacca aatcaaattc gcacgacaac ggtttcagca
      841 caatattttc cgcgtgaaca tcgccgctat cgatgccgaa agtgcgcgta ttcaaattaa
      901 gcgttgccgt tttgaagaca gccatattgg catcttcttg aggaatcagt gcaatgccgt
      961 ctgaacataa accttggtaa atctcgcttt tcgctttggc aatatcgccc actccgtcga
     1021 aaccgcagcc gacatgggcg cgcatggcgt tgttgaccaa tgcggcattt ggtttggcga
     1081 tttgcgttaa aaccgccagt tcgccgaaat ggttcatgcc catttcaatc acggcatagc
     1141 ggtgtttttc gtttaacttc aacaaagtca gcggcaatcc gatatggttg ttgaagttgc
     1201 ctgccgtcgc caacacggca tcatcgccga aacggcggcg caataccgca gccagcattt
     1261 ccttcaccgt cgtcttgccg cccgaaccgg taatgccgaa cacaaacgga ttcacatttt
     1321 cacgccacgc ctttgccagc gtttgcaatg cggcaagcgt gtcatcgact ttcaacgcgc
     1381 catccattgc agcacaatct tcgcgcgaaa ccacaaccgc cgccgcacca gcagccaata
     1441 cgtcttcaac aaaatcatgc gcgtcaaacc gctcgcccgc caatgcgaaa aacacatcgc
     1501 ccgcgcggat gtcgcggctg tcggttacga tgcgcgacac gggtttgctt tcagacggca
     1561 tcggaagctt gagggcttgg cagatgaaat ttaggtccag tggtttcata tttactttcg
     1621 ttaatattcg ggcggcggac acatcggtag cggctgattt ttttatcgcc tgttttgctg
     1681 tggtaaaaca cagattattt tcccattctc attcggcatt ttttctgtac gtatcatttt
     1741 ttagacgtat ttttagtcga tttgcctttt cccgcatacc acggcgcggg gtcgtcgggc
     1801 agtccgtcga taaaggcaag gttattgcct tcgccctgca catcgggaac attcccccaa
     1861 aaatcatagc cgtcatcggg caactcgtcg gtttcgatac ccgtccaact gccgaatccg
     1921 cgtaaaaaat taaacgcgct gcgcctcact ttaatcattt cgcgccgcgt gtcatcggac
     1981 gaaattttaa ccagcggcac gccgtagctt tgacgcttcc acgcaccatg tgtcaacgtt
     2041 cgctcgccgc ctttaccgac atgcatcaaa ccgtggtcgg aaaagtaaac caaagaccag
     2101 ctttctttat tttcattcaa tatcttaact gtatcttcta aaaatttatc ggtttgcgcg
     2161 atggtggaaa catagcagga tattttttca gtttgatact gaaaccgccg cgcatcctta
     2221 tccaaacgtg tgcaaaaatc actgtgcgaa cccatcaggt gcatcacaat cagccgaggc
     2281 ttcgtgcctg catttcctat caaaacccgt ttgaacgccg gcaacaaaag gcggtcgctc
     2341 aaccccgcgc ttttgccata atcacccctt tgggtaaacc acggataatc gctgcgtagg
     2401 gcataggtgg aaatttcgtt ggcaaaatgc cccaacattc cttgattaga cagccacgcc
     2461 gtccgaaaac ccgcctgctt cgccaagctg acgatgttat tgttcggttc tcccggcagc
     2521 cccaaagtct gcggcagcga aagattcgtc gcgtgggcgg tcgattggta accgtttatc
     2581 aacagccctt tggtctgact caaaaaaggc gtatcgggca atgggaaacc gtaaacattc
     2641 atataatccg aacgcgcgct ctcaccgata accacaacat aatttttata cttgggcgca
     2701 acatgacgga tatgccatgt tgacgctttt tttgctgctt ccaaaatgtg ggcgcgcttg
     2761 gcggcatatt gtgccggcgc ggaagccaaa tcgtaataca ggtcgaaaat attcaacaac
     2821 aggccggcat caggttctcg caaatcttta tcgctggcga ttttatccat caccgcgcag
     2881 gacaaaatca aagtcaataa tatagtcagc catattttgc tgcggcgttt atagtttttt
     2941 acgtcagcaa ataccccccc cgatacacaa tatttccaaa ctgtcaagcc aaaaatgaaa
     3001 aataatgcct gcacaaaata aagcgacccg ggaagattgc cgacaaattc acgcgcctcg
     3061 gcaggattgc tttccaatat cgaaccgact atctgataag acggcgcacc atacagccag
     3121 ccgaccggca aatatagggc ggttgtgccg acgtaaatca gtaaaacaac tgaagaaacg
     3181 cgcgggaaac cgcgtgccaa taataataaa aatacaaccg aagccaaagc acccacggca
     3241 gaataacggt agccgtattc atattccaag tgataacctg atgcgatggc cgcgccaaaa
     3301 caaaaggcta aaagcgcgca aatgcccaat gtatttttta atgtctgatt catatttttt
     3361 attcgggcgg attgtttcat acgatgccgt ccgaatatcc cattatttac gagttaacaa
     3421 agcctgtccg acgatttcaa gatcggaaaa acggtgcttc acgccttgta catcctgata
     3481 gttttcatgc cctttaccgg caatcagaat gatgtcgttt gcggcggcgc gttcgactgc
     3541 gtaacggatg gcggcggcgc ggtcggcctc gacgcattcg ggcgcgggaa cggcaggcaa
     3601 aatatcgttg atgatgtctt gtggattttc caaacgcggg ttgtcgctgg ttacgacaac
     3661 tttatccgcg ccctgtacgg ctgccgcgcc catcagcggg cgtttgcctc ggtcgcggtt
     3721 gcctccgcag ccgaatacgc accacaaagc cgcgccctgc ggtttgattt cctgcaaagt
     3781 ggcgagtgct ttttccaatg cgtcgggcgt gtgggcgtaa tcgacaacga ccaaaggttt
     3841 gccgctgttc atgatgcagt ccatgcgccc tgaagcggga cggatttttt ccaaaacatc
     3901 caatacctta tcaagcggat agccgttggc gcacagcaag gcgatgcagg cggcaaggtt
     3961 ttgcgcgttg aaccgtccga gcaggcgtgt gcggcatttc ccttcgcccc aaggggtttg
     4021 gaatacggct gctatgccgt ctgaagaagc ggtgaaatcg gtaatgcgga tatcggcttg
     4081 ctcgctgaag ccgtagctat aaacggtcaa atcggagcag tcttttttca gacggcatac
     4141 aagttccgcg ccgtattcgt catcggtatt gataacggcg tgtttcaagc cgtgccagta
     4201 aaacaggcgc gacttgatgc cgccgtaggc ttccatcgtg ccgtggtagt cgaggtgatc
     4261 tcgggtgaga ttggtaaaga ttgcgctgcg aaacggcacg ccgttgactc gcgactggtc
     4321 aaggccgtgg ctggagacct ccattgcggc ggctgttgcg ccctgttggc ggaagcggta
     4381 gagcagggtt tggacatcga cgggggcggg ggtggtatgc gtggtttctt ccaatgcgcc
     4441 ccaaaagccg ttgccaaccg tgccgataat ggcggttttt tcgcccaaca aatcggtggc
     4501 ttgtgccagc cactgtgtga tggaggtttt accgttggta ccggttacgc cccagacttt
     4561 gaggccgtct gaaacgttgc cgtaaacctc tgccgccaac atgccggcac ggtgtttcaa
     4621 atccttaatc ccttgattgg ggactttcca ttcaggattc cacgcaaatt tgccgtcgtc
     4681 gtcccaaaaa acaaaagccg cgccgttggc aatggctgcg gggatataac tgcggccgtc
     4741 ggtatattcg ccctgacagg caacgaaaat atcgccttgt ttgatttggc ggctgtctga
     4801 atgcaacaaa cgccctgccg cgtttgcaca cgacaggggc gggatgccgg tttcagccaa
     4861 aggggttaac ttgctgaaca taaaataatc tcgttgatac tcggattaag acggtgtttt
     4921 gacggctgca acattggtca gaggcttggt cggggaaacg cccaagatgt tcaggctgcc
     4981 gcccatgact tgcttgaaga ccggacctgt cactacgccg ccgtagtaac cgtttgcagt
     5041 cggttcgtcg atggttaccg ccacaatcac acggggattt ttggcaggtg caaaaccgat
     5101 gaaagtggca acgtgtttgt aatcgacgta acgtccgttg accaacttac gcgccgtacc
     5161 ggtttttgcg ccgacgtcga aaccatctac cgcacccgcc gtacccgtac cgccgggttc
     5221 ggttacagaa accatcaact cacgcacctg acgtgcggtc gatgctttaa atatacgttt
     5281 gccttgcggc gcaaccgcct gcttctcaaa gctgaccggc agcaaaacgc cgtcgtgcgt
     5341 cagtgcggta taggcgcgcg ccaattgcag caggctcaac tgaagaccat aaccgaaaga
     5401 catggttgcc tgttcgatag gccgccattt ttgccaattg cgcaacgcgc ccgcactttc
     5461 tcccggaaaa ccggaatgca tacgcacacc gatgcccaat tcatgataca aatcatacat
     5521 ttctttcgaa ctgaatcgtg cagacagttt gcttgtaccg acattggaag atttctgcat
     5581 gataccgcgc acatccaaag aaggataaac atgggtatcg cgcacctgag ccggaccaat
     5641 tttataaggc tgcgtattca acctctcgtt caaattagtt ttatccgcat ccagtgcttt
     5701 cgcaatgaca aagggcttta tcgccgaacc gggctcgatc atatcggtta cggcacggtt
     5761 gcgccgctgt tcgctgtctg catggccggg tctgttggga tcgtaggcgg gcgtgttggc
     5821 caaggcgagg atttcccccg tgcgggcatc caaaaccacc accgttccgg cttttgcctg
     5881 atggtattcg accgccttgt tcaactcttc ataggccaag gtctgaatcc tctgatcgag
     5941 ggaaaggatg atgtctttgc cgttttgcgg ggctttattg cgcggggaat ccaagctgtc
     6001 cacaatattg ccctgccggt cccgcaaaac gacttccgcg ccgtcttcgc catgcaggct
     6061 gtcttcaagc gaaagttcca aaccttcctg acctttgccg tcaatatcgg taaatccgat
     6121 gacatgtgca aacaggttgc ccatcgggta atggcgtttt aattcttttt caaatacaaa
     6181 gttttccaaa cccaaggctt taacctcttc ggcaaccttg ggatcgagct gccgcttaat
     6241 ccagataaac gacttgcctt tctgttcgag cttgttcctc aaaacatcaa ccggcacatc
     6301 gacaagctcg gacaggcgtt ccaattgtgc ggcagacggc atttccttca tctctttagg
     6361 cacggcaaac agggactccg tcggcgcact caacgccaaa accgcaccgt tccggtccga
     6421 aaccgtaccg cgtgtagccg gcaatgtttg agtccgcaca atccggttgt cgccctgttc
     6481 tttcaaaaag ttatacgtta ccgtctgcag atacagtccg cgagcaatca gaccggcaaa
     6541 caagaccgct attgccatca ggacgaagct gatccgtccg ttactggtca tcggcttttt
     6601 gacctgctct tctttgggca gcatccgagg cttatattcg ctcttaatca acatttttac
     6661 ttctcgttat tattatcctg acgcaggaat ccgattccgg cacacaggct gcttctatct
     6721 ttgatgctcc accataaagg tattgcccga aaccggcgga tggaggtttt gtttttctgc
     6781 cgccgccctg atcgcttcgt ggttcgccaa acgcgcctgt tgcagcctca tttgcgcata
     6841 atcctgctcc aaggcgattt cctgtttttt cgccttatcc aaagctgtga aattgagcct
     6901 gtactggttt tgctgcatca caacggaaaa agcggaaacg cacaccgcaa gcagcagaag
     6961 gaaattcaat ttgttcattg ccattcagac gtttttctct gtgattgttc cggtatcgga
     7021 ccggcagtcc gctccgccac acgcaaaacc gcacttctcg ccctcggatt ggcggcaatt
     7081 tccgcctcac ccggctttaa tgccctgccc acgattttca ggggcagctc gggcaaatcc
     7141 gcttccctga ccgccgccca gcgcggcagg ggcgcgtgtt gcgaatattt tttgacaaac
     7201 tgcttcacaa tgcgatcttc caacgaatgg aaagcaatga ccgccaaacg tccgccctct
     7261 ttcagacgac acatgacctg cggcaatact gcccctactt cttcaagctc gcggttaata
     7321 aagatgcgga ccgcctggaa ggtgcgcgtc gcaggatcct gcccccgctc gcgagtacgg
     7381 acgttttgtg ccacgatctg cgccagcttg cgggttgtat cgattggact ttccgcccgt
     7441 tgcgcaacaa tggcgcgcgc aatccggcgg ctaaaccgct cttcaccata attcttgatt
     7501 acctcgtgca aatcctgttc cgacgcaacc gctatccact ctgcggcaga cataccgcgc
     7561 gtcgtatcca tacgcatatc caaaggggca tcgaaacgga agctgaaacc gcggctgccg
     7621 tcatcgattt gcggggacga aatccccaaa tcaaacagcg caccgtccac cttgccgata
     7681 cccaaaccgt ccaatgccgt ctgaaacgaa gcaaaaccgc catgcacgac accgacccgt
     7741 ttgtccgaac gcgccagctc ttctgccaca gcaatcgcct gcgggtcttt gtcgaaaaca
     7801 atcaaccgcc ccgcatcgcc caaacgcgac aaaatcagcc gggaatgccc tcccctgccg
     7861 aacgtaccgt ccacatagac accgtcttcg cgcacggcaa gcgcatccac cgcctcattc
     7921 agcaagaccg tgatatgccg gtaactttct gctccactca caattgcaaa tccgtctgac
     7981 tcaactggaa ggcaagttcg tcaggatcgt catccaaagc ctgaaccatc tcagcctccc
     8041 actgctcgcg accccaaagc tccaaacggt tggcacgacc gaccaaaacg acttcacggt
     8101 cgaaatccac cctcttcctc agtccggcag aaaccagcac ccggccggcg ctgtcccatt
     8161 ccaaaatttc cgcgttatgc agcaaaagat tttgaaaccg ccgcaaaaca gggttatccg
     8221 ccacttttaa gtttaaaagt tgcgccgcaa ccttttccca ctccgcaaca gggtacatca
     8281 acagcttgtg tttcgactcg agcgttacca ctacggcagg cgtatagagg cgcgacagaa
     8341 tgtcacggaa tttggcagga acagccaacc gccccttact gtcgatgctt aattcgtgtg
     8401 cgccgccgaa catgacatgt cccaagccga aatcaaaatc acaagggtaa aagagacact
     8461 ttgccccaca attccccacc aatcgacact ataagaaatt ttaaacactc ggtcaaatca
     8521 gggcatgaaa actcattaac atatctgaaa tttttattcc ttttaaaaac aataagataa
     8581 aaaatgacga caacggcacg gcgggtgcgg tacagaataa tcgaaccaat aaacaactat
     8641 atatgattaa tttaataata taaacacaat atatagtatt aagataaagc catgacagca
     8701 cccgtaccaa cgtgtaatat gtcgggaaat ccaataaatt tacacaagct aacacttatc
     8761 atgcccctcc cctctcccga agcacggcaa ttctcgctca aactgcaaac cctcattgcc
     8821 gaaaaaatcg gcaaacacgg caactggatt ccattttcac gttttatgga attggtttta
     8881 tacgctccgc aatacggcta ctacaccggc ggcagccata aaatcggcaa taccggggat
     8941 tttattaccg caccgaccct cacctctctg tttgcacaga cactggcacg ccaacttcaa
     9001 gaacttctat cccaaacggc gggcaatatc tatgaattcg gcgcgggaac cggacagctt
     9061 gccgccgatt tgttgggcag catttcggac ggcatcagcc gttactatat tattgaaata
     9121 tcgccggagc tggcagcacg tcagaaaaac ctgattcaag cacgcgcacc ggaagcatct
     9181 caaaaagttg tccacttgac cgcacttccc gaagcgtttg acggcatcat catcggcaac
     9241 gaagtactcg atgccatgcc tgtcgaaatc gtccgtaaaa atgaaggcgg ctcattcgag
     9301 catgtcggcg tttgcctaga taatgaccgt tttacctatt cggcacgacc gctgcacgac
     9361 ttgcagctat ctgccttggc ttccctctat tttcctcaaa cagattatcc ctataccagc
     9421 gaactacatc cgcaacaata tgcctttatc cgcacccttg cctcaagact cgaacacggc
     9481 tgcatgatat tcatcgacta cggttttgat gcagcgcagt attaccaccc tcaacgcaat
     9541 caaggtactc tgatcggaca ctaccgacat cacattatcc acaatccttt tgacttcatc
     9601 ggattggccg acctgaccgc acatgtcaac tttaccgaca ttgcacaagc agggacggat
     9661 gccggattag atttgatagg ttaccttccc caatcccatt tcttattgaa cttgggcatt
     9721 accgagctat tggcacagac ggggaaaacg gattcggcag cctacatctg tgaagctgct
     9781 gccgttcaga aactgattga ccagcatgaa atgggcgaac tgtttaaagt catcgcattc
     9841 ggaaaaaata tcggcatcga ctgggcagga ttccgcttcg gcgacatctg ccacaaactc
     9901 taaccctcat gccgcctgaa tccgcttcag acggcataaa ctttttaaca tttaaaaaca
     9961 gtcaactaat tcaaaattaa aaaatacggc ttgtcaaaaa aacagaaaaa catataatag
    10021 cgtcttcacg aaacggcgaa ttagctcagt cggttagagc agaggaatca taatccttgt
    10081 gtccggggtt cgagtccctg attcgccacc aaattttcgg gggtatagct cagttggtag
    10141 agcgcttgca tggcatgcaa gaggtcagcg gttcgatccc gcttacctcc accagataaa
    10201 aaagcacaga ccgtaaaaag gtatgtgctt ttttattgcc tgattgccag caaataaaga
    10261 ataaaccact gccttcaaaa caggcaatcg actttaaacc tatcgccccg cctgtcctga
    10321 ttttatagtg aattaaattt aaaccggtac agcgttggct cgccttgccg tactatctgc
    10381 ggcttcgtcg ccttgtcctg atttttgtta attcactata tcagcccgcc agacaaaccc
    10441 gacccgaata atgtcttcag gtcgggttta tggtttcatt cccaacttat ccagcctgac
    10501 agccacaata taatgatggc aataccaaaa acaatgcggt aataggcaaa aggaatataa
    10561 tttttcttgg aaacaaacct cagcaacgct tttaccgcta ccaagcctga aacaaaggca
    10621 gcaataaagc ctatcagaat caaaccgaca tcatgcaggg tgaaaaatcg gtaatgtttc
    10681 aggacatcat aagccgttgc ggcaaccatc atcggcacag ccaagaaaaa cgagaattct
    10741 gtcgcagttt tccgttcgat gccccaaagc atcccgccca taatcgtact gcccgaacgg
    10801 gacgtacccg gaaccagtgc aaacacttgg gcaacgccga tcatcaaggc atcaatcgga
    10861 cgcaatgcat caacatcggc aattttaggc tctgctcggc tttggcgttt ctccacccac
    10921 aaaataaaaa aaccgcccaa aaccagcatg actgcaacac tcaaggggtt aaacagatac
    10981 tctttgattt gtttgccgaa caacagcccc atcacggcgg caggtataaa agcaatggca
    11041 agattaagga cgaagcggtt ggctttccgg tcttttccca agccgtgcaa cacattgctg
    11101 aaacgttgcc ggtattcaaa cactaccgcc aaaactgcac cgagctggat ggcaatttca
    11161 aaaaccttgt gattgctgtg aaaaccaatc agattgccga acacaatcaa atgtccggtg
    11221 ctggaaatcg gtaaaaattc ggtaaaacct tctaccaagc ccatcatcag ggctttcagg
    11281 acaatcagaa aatccattgc ttgcgcttct ttcggatacg ggagttcggc tatttctgta
    11341 cagcaggggt ctgacgcttg cgttcttccc tgactttggc aaccaattct ttaatcgtat
    11401 gaacgccgcc gtcaaagccg ttattgaaga taacgcggta ttttccgccg acaataacgg
    11461 tcggcgtgct gtcgatgcgg tattgttccg tcagtttctg catttttaat gcggcggcgg
    11521 cagcttcggg ggaatcatag gcgcgcatca gttttttgcc gtcaaagcct ttttgagaca
    11581 aagcccattt tccggcaacc gacctgtttt ccaagcggat tttttgttcg taaactgctt
    11641 taaacacagc agggtttgcc tgatatttca aacccgacaa attgacggca gccgccatcc
    11701 tagccaaacc gagcatttca ggctgccaga ccacgtgctc cgtcctcaaa taggcatcag
    11761 acggcaatgc cttgcccagt ttcaataaca aaggatcgaa atgatggcaa tgtacgcaga
    11821 aatagccgaa aaattccaaa acctcaattt tacccgactg ttcttgagga atgggtttat
    11881 ccaacacaag atagtcttcc ccttccgtca gggcatatgc ctgcgcggac aacactgccg
    11941 acagcagcag cggcaacaga tgtttgagct tcataattat ttgctttcga tagaacggat
    12001 caggctggcg acttcatgtt ttttcaactc gtcctgcatt tttttcaccg catcggcaga
    12061 catattgccg ctttgcaccc ggtaaagcgt tttatgtccc gcctgataac cgaccacctt
    12121 ggaagatatg cccaagattg ccagtttggc acgctgccct tccgcgctct gacggtcggc
    12181 atacgcgccc atttgcagat aatgcgttgc ttccgccttg tcggacgttt tcattttctg
    12241 cacttctttg gcggcggcac tgcgcgcttt ttcgatgctg ccgctgttga ggatttgttc
    12301 cggggttggt ttgggtgcaa ctttttcctt cgccgccttt ttctcttctt ttgaagcttt
    12361 tttctctgtt tctttagacg gttttaccgc ttgtttttta accgtttcgg catctttctt
    12421 ctgcgctttt tccctgacgg tttgttcacg ctcttccgtc agcgctttct tacgcactgc
    12481 ctgtccgtcc ggctcttccc gttccggctc gcccgccttt tcttcaacct cgtcggcttt
    12541 atcggcaacg ggctgcttgt cggcagcttt ttccgcatcc gactgctctg cctctgtcgc
    12601 agcatccggt tcggacaagg cgttttgatc ggccggttca ggttggatgt cttccttagg
    12661 ctggtttttc ggtttcagga tttccgtttc tgcaggctgc ttcgacgaag ccgggatttt
    12721 gaacgcattt tgaccgctct ggttcagata aaacaaaata ccggcaataa tgaccgtcgc
    12781 cagtatcaaa ccgaagaaaa aaccggacag accttttccg gattgggaaa atttgttcat
    12841 aaacatacct taatgtgttt cagacggcat taacgccgtt tgcttacggg cggatattct
    12901 aacaatatcg ccatatttgg gcaaaacctg cttccattcc cattcctata aagcacgacg
    12961 gaaacctatg gctttcatga cgtggcttct gccgacttct tcgtcgcccg ccaaatccgc
    13021 caatgtacgc gccacgcgca taatgcggtg gaagctgcgg gcggaaaggg agagtttttc
    13081 cagcaggccg cccaatgctt cctgcgcttc tttttgaatg cgggcggatg tgtcgagttc
    13141 actgacactc aaggcggcat tcactttgcc ttgccgtgcg tattgtttgt ctctagcggc
    13201 gataacgcgt tccaaaacgg acgcgctgct ttcccctgct tcctgctgca tcagttcggc
    13261 ggcggacagg ctcgggactt cgatggtcaa atcgatgcgg tcgagcagcg gcccggaaat
    13321 cttgctgcgg taacgcgcga cgctttcggg cgtgcagcgg cagggtttga cgggatgccc
    13381 gagataaccg cacgggcagg ggttcatggc ggcaacaagt tggaatttgg caggatagac
    13441 ggcttggcgc gccgcgcggg aaatgtggat ttcgccgttt tccaacggtt cgcgcaaaac
    13501 ttccaaaact ttgcggtcaa actcgggcag ctcgtccaaa aacaaaacgc cgtggtgcgc
    13561 caatgaaatt tcgcccggac gcggatccga accgccgccg accatagccg ccgcgctggc
    13621 gctgtgatgc ggactgcgga aaggacggtt gctgtcgagt tgttgttggt ggttgggcag
    13681 gagcgaacgc aatgcccaaa cttctaccaa ttcgtcttcg gtcagcggcg gcaggatgcc
    13741 gggcagccgt tgggagagca tagacttgcc cgttcccggc ggacccatca tcaagaggct
    13801 gtgtccgcct gcggcagcga tttccaaagc aaggcgcgcg gtgtgctgac ctttcacatc
    13861 gcacaaatca ggttgtccgc catgttcaaa cggcatctga ggaacttggc attcggtttg
    13921 cgccaaaggt tcgatgccgt tcaaatgggc ggcgacttcg cccaaagagc gcgcgccgta
    13981 aacggtaatg ccgcgcatca cggcggcttg tcctgcattt tcttcaggca aaacaaatgc
    14041 acgttttgcc tgcataccct gccacgccat cgccaacgcg ccacgcacgg ggcgcaacag
    14101 ccccgacagt gccaattccc ccgcaaactc gtattcctcc agtttttcgg gcgcaacctg
    14161 ccccgatgcg gcaaggatgc cgattgcaat cggcaaatcg aaacgccccg actctttggg
    14221 caggtcggcg ggggcgaggt tgacggtaat ttttttggcg gggaattcaa aaccgctttg
    14281 aataatggcg gcacggacac ggtcgcgact ttcctttact tccatatcgg gcagtccgac
    14341 gatgttgaaa tgtggcaggc cgttggcaag gtgggcttcc acttcgacca acggcgcatt
    14401 cataccgctc aaggcgcggc tgtaaaccaa ggcaagcgac atatttcaga cgaccttatt
    14461 cgccggcttc ggtttgctgc ctgatttcgg cgacggcttc ttcggcagcg gcttcagccg
    14521 cttccaatgc tgcccgttcg ggattttgcg cggcttcgag tttttccaaa cgcgcttcca
    14581 aagccgccag tttggtacgg gttttgatta aaacctgctg ctggatgtcg aattcttcgc
    14641 gcgtaaccag atccatacgg ttgaacgcgc cgcccagcat cgccttaata tttttttcca
    14701 catctttggc agggctgttg gcgatggttt cgctgatttt cgagccgact tcctcaaaaa
    14761 gctgcttgcc gaacataatc tgtatccttc ctgaacatat caaattcaat cggctattgt
    14821 ataaggaaaa atgccgtctg aaaacgggcg gcggataatc ggcaaaacat accgcgcctc
    14881 ctttgcggtt gctaaattac acctcaaaac acctgccgcc tgaaaacgga tttcatattc
    14941 cgcgcaacgc ccgattacaa gacactacaa aacaatatgc tgttttaaat gatttttccg
    15001 acgcgcatcg tttcaaactc ggctttttaa gccattaagt gctttgcaaa caacagaaat
    15061 tgggttatcc tgaaacggat tatttacaat ttcatatagt tttattacat atcttattgt
    15121 gattgaagat aatttatccg aatccccctt tcgggtatcc ggattttccg ttgtactttt
    15181 attagaaaaa catttcaggc gcaagttgct tgcaatttca aagcctgtaa ttctgaagtt
    15241 ttagacgagg aatagcacat gaatcccatg tacattactt ttgcaatcta tctggttgca
    15301 gtccttctca tcgggcttgc cgcctatttt tctacgcgca atttcgacga ttatattttg
    15361 ggcgggcgca gcctaggccc gtttgttacc gcgatgtcgg caggcgcgtc cgatatgtcc
    15421 ggctggcttt tgatgggtct gccgggcgcg atttatttga gcggtttgaa tgaggcttgg
    15481 attgccatcg gcctcttggt cggcgcgtat ttcaactggc ttttggtggc gggccgtctg
    15541 cgcgtacata ccgaatacgc caacaacgcg ctgacgctgc cggattattt cttccaccgc
    15601 tttggcgcgg gcggacactt gatgaaagtg gtttccgcac tgattatcct gtttttcttc
    15661 acgatttatt gcgcctcggg cattgtggcg ggcgcaaccc tgttccaaag cctgtttgaa
    15721 ggtatgactt acaatcaggc aatgtggctg ggcgcgggcg cgaccatcgc ctataccttc
    15781 ttgggcggct ttttggcggt aagctggacg gatacgctgc aggcttcttt gatgattttc
    15841 gcgctgattt taacgcctgt gatggtctat ctgggcttgg gcggcgcgga acagatgtct
    15901 gccgcgattc aaagtgtcgc cgcaggcacg ggcaaagaat acggcagcct gtttgccggt
    15961 acgaccgtca tcggcatcat ttccaccgcc gcatggggct tgggctattt cggacagccg
    16021 cacattttgg cgcgctttat ggcggccgaa agcgcgaaat cgctggtatc cgcacgccgc
    16081 atcggtatga cttggatggc gttgtgcctt gcgggcgcgg tagcggtcgg ttatttcggc
    16141 attgcgtatt tcggtgcaaa tcccgacaaa gtttcttcta tgagcggcaa ccacgaacgc
    16201 atcttcatcg cgctttccac cctgctgttc aacccttgga ttgccggcat tattttgagc
    16261 gcgattttgg cggcggtgat gtccaccctg tcctgccagc ttttggtttg ctccagcgcg
    16321 attaccgaag acttctacaa aggctttttg cgtaaaaacg cgcaacagtc ggaattggta
    16381 tgggtcggcc gcctgatggt cttggcgatt gccgtgattt ccatcctgat tgcttccgat
    16441 ccgaacagca aagtattggg actggtgtct tacgcgtggg caggtttcgg cgcggcattc
    16501 ggcccgattg tgattctgtc cgtattgtgg aaacgcatca ctgcctacgg cgcgttgtcg
    16561 ggtatggttg caggtgcgtt aaccgtagtc gtatgggcgg aatgggtgaa aaaaccggct
    16621 caagcggcag gagaaagcgg cttgttgacg atgtacgaaa tcgtgccggg ctttattgtt
    16681 tgcttgatcg ttgccgtgtt ggtttccctg ttcaacaaag aaccttcgcg cgaaacccaa
    16741 gaacgctttg aaaaagccga cgcagattac cgcgccaccc gataaacccc tttcagacgg
    16801 cacggcatcc cggttcgtgc cgtctgaacc cgtttccggg tcaaacacaa ataaaccttg
    16861 cctgcataca ggccttattt gtgttttacc cgtttcattt tcaaagaaac ggaagcacct
    16921 accctgcccg catttgaagc cttgtatttc atctgcggat ttattacgat attaccgtat
    16981 tcaggccgca ccgatgccgc ctgccccccc gaaaaacttt ggagaatcca aaaaatgttt
    17041 cattttgcat ttccggcaca aactgccctg cgccaagcga taaccgatgc ctaccgccgt
    17101 aatgaaatcg aagccgtaca ggatatgttg caacgtgcac agatgagcga cgaagagcgc
    17161 aacgccgcct ccgagcttgc ccgccgtttg gttacccaag tccgcgccgg ccgcaccaaa
    17221 gccggcggcg tggatgcgct gatgcacgag ttttcactct ccagcgaaga aggcatcgcg
    17281 ctgatgtgtc tggcagaagc cctgctgcgt atccccgaca acgccacgcg cgaccgcctg
    17341 attgccgaca agatttcaga cggcaactgg aaaagccatt tgaacaacag cccttccctc
    17401 ttcgtcaatg ctgccgcctg gggcctgctg attaccggca aactgaccgc cacaaacgac
    17461 aaacaaatga gttccgcact cagccgcctg atcagcaaag gcggcgcacc gctcatccgc
    17521 caaggcgtaa attacgccat gcggcttctg ggcaaacagt tcgtaaccgg acagaccatt
    17581 gaagaagccc tgcaaaacgg caaagaacgc gaaaaaatgg gctaccgctt ctccttcgat
    17641 atgttgggcg aagccgccta cacccaagcc gatgccgacc gctactaccg cgactatgtc
    17701 gaagccatcc acgccatcgg caaagatgcg gcaggacaag gcgtttacga aggtaacggt
    17761 atttccgtca aactttccgc catccatccg cgctactcgc gcacccaaca cggccgcgtg
    17821 atgggcgaac tgttgccgcg cctgaaagag ctgttccttt tgggtaaaaa atacgatatc
    17881 ggtatcaaca tcgatgccga agaagccaac cgtctggagc tgtctttgga tttgatggag
    17941 gctttggttt cagaccctga cttggctggc tacaaaggta tcggtttcgt tgtccaagcc
    18001 taccaaaaac gttgtccgtt cgttatcgac tacctgatcg accttgcccg ccgcaacaac
    18061 caaaaactaa tgatccgcct cgtcaaaggc gcgtattggg acagcgaaat caaatgggcg
    18121 caagtggacg gcttgaacgg ctatccgacc tacacccgca aagtccacac cgacatctcc
    18181 tacctcgcct gcgcgcgcaa actgctttcc gcgcaagacg cggtattccc gcaatttgcc
    18241 acccacaacg cctacacttt gggcgcaatc taccaaatgg gtaaaggcaa agattttgaa
    18301 caccaatgcc tgcacggtat gggcgaaacc ctgtacgacc aagtcgtcgg cccgcaaaac
    18361 ttaggccgcc gcgtgcgcgt gtacgcccca gtcggcacac acgaaaccct gctcgcctac
    18421 ttggtgcgcc gcctgttgga aaacggcgcg aactcgtctt tcgtcaacca aatcgtcgat
    18481 gaaaacatca gcatcgacac gctcatccgc agcccgttcg acaccatcgc cgaacaaggc
    18541 atccacctgc acaacgccct gccgctgccg cgcgatttgt acggcaaatg ccgtctgaac
    18601 tcgcaaggcg tggacttgag caacgaaaac gtattgcagc agcttcaaga acagatgaac
    18661 aaagccgccg cgcaagactt ccacgccgca tccatcgtca acggcaaagc ccgcgatgtc
    18721 ggcgaagcgc aaccgattaa aaaccctgcc gaccacgacg acatcgtcgg cacagtcagc
    18781 tttgccgatg ccgcgcttgc ccaagaagcg gttggcgcag ccgttgccgc gttccccgaa
    18841 tggagtgcga cacctgccgc cgaacgcgcc gcctgcctgc gccgttttgc cgatttgctg
    18901 gagcagcaca ccccagcact gatgatgctt gccgtgcgcg aagcaggcaa aacgctgaac
    18961 aacgccattg ccgaagtgcg cgaagccgtc gatttctgcc gctactacgc aaacgaagcc
    19021 gaacataccc tgcctcaaga cgcaaaagcc gtcggcgcga ttgtcgccat cagcccgtgg
    19081 aacttcccgc tcgccatctt taccggcgaa gtcgtttccg cattggcggc aggcaacacc
    19141 gtcatcgcca aacccgccga acaaaccagc ctgattgccg gttatgccgt ttccctcatg
    19201 cacgaagccg gcatcccgac ttccgccctg caactcgtcc tcggcgcagg cgacgtgggt
    19261 gcggcattga ccaacgatgc ccgcatcggc ggcgtgattt tcaccggctc gaccgaagtg
    19321 gcgcgcctga tcaacaaagc ccttgccaaa cgcggcgaca atcccgtcct gattgccgaa
    19381 accggcggac aaaacgccat gattgtcgat tccaccgcac ttgccgagca agtctgcgcc
    19441 gacgtattga actccgcctt cgacagcgcg ggacaacgct gctccgccct gcgcattttg
    19501 tgcgtccaag aagacgttgc cgaccgtatg ctcgacatga tcaaaggcgc tatggacgaa
    19561 ctcgtcgtcg gcaaaccgat tcagctcact accgatgtcg gccccgtcat cgatgccgaa
    19621 gcacagcaaa acctgttgaa ccacatcaac aaaatgaaag gtgttgccaa gtcctaccac
    19681 gaagtcaaaa ccgccgccga tgtcgattcc aaaaaatcca cgttcgttcg ccccatcctg
    19741 tttgaattga acaacctcaa cgaactgcaa cgcgaagtct tcggtcccgt cctgcacgtc
    19801 gtccgctacc gcgccgacga actcgacaac gtcatcgacc aaatcaacag caaaggctac
    19861 gccctgaccc acggcgtaca cagccgcatc gaaggcacgg tacgccacat ccgcagccgc
    19921 atcgaagccg gcaacgttta cgtcaaccgc aacatcgtcg gcgcagtcgt cggcgtacag
    19981 cccttcggcg gacacggtct gtccggcaca ggccccaaag caggcggttc gttctacctg
    20041 caaaaactga cccgcgccgg cgaatgggtt gccccgaccc tgagccaaat cggacaggcg
    20101 gacgaagccg cactcaaacg cctcgaagca ctggttcaca aactaccgtt caacgccgaa
    20161 gagaaaaaag ccgcagcggc cgctttggga cacgcccgca tccgcaccct gcgccgtgcc
    20221 gaaaccgtcc ttaccggacc gaccggcgag cgcaacagca tctcatggca cgcgcccaaa
    20281 cgcgtttgga tacacggcgg cagcacggtt caagcctttg ccgcactgac cgaacttgcc
    20341 gcctccggca tacaggcagt ggtcgaaccc gacagcccct tggcttccta cactgccgac
    20401 ttggaaggtc tgctgctggt caacggcaaa cccgaaaccg ccggcatcag ccacgttgcc
    20461 gccctgtcgc ctttggacag cgcgcgcaaa caggaacttg ccgcccacga cggcgcactc
    20521 atccgcatcc tcccttcgga aaacggactc gacatcctgc aagtgtttga agaaatctct
    20581 tgcagcgtca acaccacagc cgccggcggc aacgccagcc tgatggcggt cgccgactga
    20641 ttttgccgaa atacccgggc gcggcccgtg aaccaatgcc gtctgaaaac ctttcagacg
    20701 gcatttttat a
//
LOCUS       DAWIVB010000017        19917 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWIVB010000017 DAWIVB010000000
VERSION     DAWIVB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.017"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WIX60_000436"
     CDS             complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WIX60_000436"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ5304753.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGSVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCCLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(3080..3868)
                     /locus_tag="WIX60_000437"
     CDS             complement(3080..3868)
                     /locus_tag="WIX60_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ5304754.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFVGGVAGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(4021..5175)
                     /gene="prpC"
                     /locus_tag="WIX60_000438"
     CDS             complement(4021..5175)
                     /gene="prpC"
                     /locus_tag="WIX60_000438"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ5304755.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5261..6136)
                     /gene="prpB"
                     /locus_tag="WIX60_000439"
     CDS             complement(5261..6136)
                     /gene="prpB"
                     /locus_tag="WIX60_000439"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ5304756.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6762..8072)
                     /locus_tag="WIX60_000440"
     CDS             complement(6762..8072)
                     /locus_tag="WIX60_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ5304757.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(8211..9389)
                     /gene="ftsZ"
                     /locus_tag="WIX60_000441"
     CDS             complement(8211..9389)
                     /gene="ftsZ"
                     /locus_tag="WIX60_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ5304758.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9508..10752)
                     /gene="ftsA"
                     /locus_tag="WIX60_000442"
     CDS             complement(9508..10752)
                     /gene="ftsA"
                     /locus_tag="WIX60_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ5304759.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10838..11566)
                     /locus_tag="WIX60_000443"
     CDS             complement(10838..11566)
                     /locus_tag="WIX60_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ5304760.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11556..12470)
                     /locus_tag="WIX60_000444"
     CDS             complement(11556..12470)
                     /locus_tag="WIX60_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ5304761.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12583..13992)
                     /locus_tag="WIX60_000445"
     CDS             complement(12583..13992)
                     /locus_tag="WIX60_000445"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ5304762.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYLEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLALSKQI"
     gene            complement(14147..15214)
                     /gene="murG"
                     /locus_tag="WIX60_000446"
     CDS             complement(14147..15214)
                     /gene="murG"
                     /locus_tag="WIX60_000446"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ5304763.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGGLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15218..16543)
                     /locus_tag="WIX60_000447"
     CDS             complement(15218..16543)
                     /locus_tag="WIX60_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ5304764.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16669..18006)
                     /locus_tag="WIX60_000448"
     CDS             complement(16669..18006)
                     /locus_tag="WIX60_000448"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ5304765.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQSDIEAFKQNGGRVLGDIELLA
                     DIVNRRDDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIVTQDIPLQGLHN
                     AANVMAAVALCEAIGLSREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLGEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(18090..18686)
                     /locus_tag="WIX60_000449"
     CDS             complement(18090..18686)
                     /locus_tag="WIX60_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ5304766.1"
                     /translation="MKQTVKWLAAALIALGLNRAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRRDDAEAVRWYRQAAEQGLAQAQYNLGWMYANGRGVRQDDTEAVR
                     WYRQAAAQGVVQAQYNLGVIYAEGRGVRQDDVEAVRWFRQAAAQGVAQAQNNLGVMYA
                     ERRGVRQDRALAQEWFGKACQNGDQDGCDNDQRLKAGY"
     gene            complement(18812..19894)
                     /locus_tag="WIX60_000450"
     CDS             complement(18812..19894)
                     /locus_tag="WIX60_000450"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ5304767.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4736 a         5566 c         5010 g         4605 t
ORIGIN      
        1 cgccttgtcc cgatttttgt taatcctcta taccatgtca attcagcctt tatttcgcgg
       61 gcaaagccca cgctacaccc actttccaga agtacattag gctgccactc cgacaaagcc
      121 gtttgattcc gcaaactgtc ggggttcgcc acaatctacg gctactgtgt tgtgatacgg
      181 cagattatta aaccccgtca ttcccgcgca ggcgggaatc caaatttgtc cgcacagaaa
      241 ctcatcggat aaataaggtt tcctcaattc cactttctgg attcccgcct gcgcgggaat
      301 gacgatttac agtagatgcc tgccatatcg agaattacgt gaatgagcaa aatgttgaac
      361 ccgacccacg ctacgtcttg cttttcagac gacctctaag ccgcgttccc ttccaaaaaa
      421 tcctgtgcaa accgttgcaa cacgccgccg gcttcatata ccaatacttc ttctgcagta
      481 tcgaggcagc aggtaacggg aacttcaacg gtttcgccgt ttttacggtg aatcacgagg
      541 gtcaggtcgc agcgcggtgt gcgttcgccg accacgtcgt aggtttccgt accgtccagt
      601 tgcagggtat ggcggttggt gtcgggtttg aactgcagcg gcaacacgcc catgccgata
      661 aggttggtgc ggtggatacg ctcgaagcct tcggcaacaa tcgcttctac gccggcgagg
      721 cgtacgcctt ttgcagccca gtcgcggctt gagccttgac catagtccgc accggcaatg
      781 atgatgagcg gctgtttgcg gttcatatag gtttcgatgg cttcccacat gcgcatggtt
      841 tcgccttcgg gttcgacgcg ggcgaacgag ccttggcgca cgctgccgtc ttcgtttttc
      901 accatttcgt taaacagttt cggattggcg aaggtagcgc gttgggcggt caagtggtcg
      961 ccgcggtggg ttgcgtaaga gttgaagtct tcttcaggca aacccatttt cgccaaatac
     1021 tcgcctgcgg cactgacggc caaaatcgca ttggacggcg agaggtggtc ggtggtgatg
     1081 ttgtcgggca aaatcgccag cggacgcata cctcttaatg tgcgttcccc tgccagcgcg
     1141 ccttcccagt aaggcggacg gcggatgtag gtggacatcg gacgccaatc gtacagcgga
     1201 ctgggtgctt tttgcgctgt gccggtgtcg aacatcggta catacacatc gcggaactgc
     1261 tgcggtttca catattcggc aacgacggca tcgatttctt catcggcagg ccaaatgtct
     1321 ttcaggcgga tttccttgcc gtctgcaacg ccgagtacgt cgttttcaat atcgaaacgg
     1381 atactgcctg ccagcgcgta ggcaacgacc aacggaggcg aagcgaggaa agcctgtttc
     1441 gcatacgggt ggatacggcc gtcgaagttg cggttgcctg ataatacggc ggtggcgtac
     1501 aaatcgcggt cgatgatttc tttctggatt ttcggatcca gcgcgccact catgccgttg
     1561 caggtggtgc aggcgaaggc gacgataccg aagccgagtt tttccatttc gggcaacagg
     1621 cccgcttctt tcaaatagat ttcggctact tttgaacccg gggcaaacga agatttcacc
     1681 caaggtttgc gtttcaagcc gagacggttg gcattgcgtg ccaagagcgc ggcggcaaca
     1741 acgttgcgcg ggttggaagt gttggtgcaa ctggtaatcg cggcgatgat gaccgagccg
     1801 tcgggcattt ggccgtccga aggctcttcg taaggcttcg ccagcccttt cgccgccaaa
     1861 tcggcggtcg caaaacgggc atgcgggtta cttgggcctg ccatattgcg cgttacgctg
     1921 ctcaaatcaa atttcaaaac gcgaggataa acggcggttt tcaaggcatc tgcccacaag
     1981 cctgcggttt tggcgtaggt ttccaccaat ttcacctgcg cgtcgtcgcg tccggtcagt
     2041 ttcaaataat caatggtttg ctcatcaata gcgaacatcg cggcagtcgc gccgaactcc
     2101 ggcgtcatgt tggaaatggt cgcgcggtcg ccgatagaca ggcttctcgc gccctcgccg
     2161 aagaattcga caaacgcccc gaccacgcgt tctttgcgca gaaactcggt cagtgccaac
     2221 acaatatccg tcgccgtaat gcccgcctgc cgtttgccgt tcagctcaac gccgacaata
     2281 tcgggcaggc gcatcatgga cgcgcgtccc agcattacgg tttccgcttc caatccgccc
     2341 acgcccacgg aaatcacgcc caatgaatcg acgtgcggcg tatgtgagtc agtaccgacg
     2401 caggtatcgg ggaaagccac gccgtttttg acttggacga cgggcgacat tttttctaga
     2461 ttgatttggt gcatgatgcc gttgcccgcc ggaatcacgt ccacattttc aaacgcggtt
     2521 tttgtccagt tgatgaagtg gaaacggtct tcgttacggc ggtcttcgat ttcgcggttt
     2581 ttgcggaagg catcaggatc gtaaccgccg cactccaccg ccagagagtg gtcgacgatg
     2641 agctgggttt gcaccaccgg attcactttg gcaggatcgc cgcctttttc ggcaatcgca
     2701 tcgcgcaggc ctgccaaatc caccaacgcg gtctgcccca gaatatcgtg gcacaccacc
     2761 cgcgccggat accacggaaa gtcgatttcc tgcttccctt ctatcaactg ccccagccag
     2821 ctttgcagcg tcggcaaatc gactttgtcc gcgcggttga ccaaattctc cgccaaaatg
     2881 cggctcgtgt aaggcagctt gtcgtaagag ccgggcttga tgtcctcaca cgccgcacgc
     2941 gcgtcgtagt attccaaatc cgtaccgggc agcggtttgc ggtaacgttg gttggcagcc
     3001 atgtcggttc tcctgtggat ctgtttttct tgtggtttga ggtttcagcc gacgttttga
     3061 aggggtcgtc tgaaagggtt taaaacatcg aaacaatcat ctgatacagc ggatttctct
     3121 cgtctatcaa caatttcaca gccatcgaaa tgctgatgac aatcagcagc ggcttaatca
     3181 gcttcgaacc gaagcggacg gcaaatctcg cacctaaatt cgcaccgaca aacgcaccga
     3241 ccgccatcgt tgccgcaatc gggaaaataa tcgaaccgtg cagcaggaat accgatagcg
     3301 aaccaagatt gcaggcaacg ttcgccaatt tggtgtaaga catcgcgttc aacagcttgc
     3361 agccgagcaa aacaataaag gcaatcagaa aaaacgagcc gacacccggt ccgaacacac
     3421 cgtcgtaaaa acccaaaagc ggtgcgaccg tcagcccgaa cagaaaaaaa gacattctgg
     3481 ctttgccttc cttactgccg tcgagcttgg gcgaaaacac aaaatacagt gcgacaaata
     3541 tcaacaaaac cggcacgacc gccagcagaa tatctttgga aaccaagctg accgataatg
     3601 caccggccac gccgcctaca aacgatgctg cggcaatcgg gagacctttc ttccaatcaa
     3661 tcaaaccttt gcgtgcaaaa gaaaccgtag ctgaaaacgt agcagcggct gcttgcagct
     3721 tgttggtggc aattgccgac acgggaggaa tacctgccaa caagagtgcg ggcagcgtaa
     3781 tcaaaccacc cccgcccgca atcgcatcga taaatccggc aatcatcgca accaaaccca
     3841 aagcgagtat tatatataaa tcttccatgt ttcttatcct gttacttgcg ccaatacagg
     3901 attatcttct cctattagat taaacttatt tcagacaacc tttccaataa ggcaaggtcg
     3961 tctgaaatcc ttagctttgc ataccgaaat taaagaacaa actgctattg cattcttcaa
     4021 ttatcgttct tcaatctcca caaacgccaa atcttcaggg cctgtgtagt ttgcgctcgg
     4081 acggatgatt ttgccgtctt tgcgttgctc aagaacgtgt gcgctccaac cggttgtacg
     4141 ggaaattacg aacagcggtg tgaacatagc ggtcggtacg cccaattttt ggtaggaaac
     4201 ggcagagaac cagtccagat tcgggaacat ttttttctct tcccacatca cgctttccaa
     4261 acgttcggca atgtcaaaga ggcgcatatc gccggtttct ttgctcaaac cgcgtgccac
     4321 ttctttaatg acaacgttgc gagggtcgga aatggtgtac accggatgac cgaaaccgat
     4381 cacgatttct ttgcggccga tgcgttcgcg gatgtcggct tcagcttcgt cggcattgcg
     4441 gtagcgtttt tgaatatcgt aagccacttc gttcgcgccg ccgtgtttcg gacctttcaa
     4501 cgcgccgatt gctccggtaa tgctggagta catatcagag cctgtaccgg cgatcacgcg
     4561 ggcggtaaag gtagaagcgt tgaactcgtg ttcggcatac agaatcagtg aaacgtgcat
     4621 ggctttgatg tgtgattcgc ttgggcgttt gccgtgcaac agttgcagga aatgaccgcc
     4681 gatggtctct tcgtcgcttt caacctcaat gcgtttgccg ttgtgcgaat attgatacca
     4741 gtacaagagg atgctgccga ggctggcgat cagtttgtcg gcgatgtcgc gcgcttcact
     4801 ttccggatgg ctttcacgtt caggatgaac gcagcccagc atggatacgc cggtacgcat
     4861 tacgtccatc ggatgggtat gtgcaggcag gctttccaaa actttaatca cacggatagg
     4921 caggccgcgc atggatttga gcttggtttt ataagcggcc agctcgaatt tgttgggcag
     4981 atggccgtga atcagcaggt gggcgacttc ttcaaactcg catttttgtg ccaaatccag
     5041 aatgtcgtaa ccgcgatagc tcaaatcgtt gccggtacgg ccaacggtac acaaagcggt
     5101 attaccggcc gcaacgccag aaagcgcaac ggatttttta ggtttgaggg tcggggtttg
     5161 agtagtttca gtcatggtat ttctcctttg tgtttttatg ggtttcgggt tttcagacga
     5221 ccgatgcgga tttgttgaaa ggcagtctga aagcggtaaa tcatttttga aacaatttat
     5281 ccagtttttg ctcgaaggca tgatagttca gatgctcgta cagctcggca cgggtttgca
     5341 tactgtccac caccgccgcc tgagtgccat cgcgcataat cgcttcgtaa acattcagag
     5401 cggctttgct tgctgcacgg aacgatgaca gcggatacag caccagcgac acgccgtttt
     5461 cagccagctc gctttgggta taaagcggag tggaaccaaa ctcggtaatg ttcgccaaca
     5521 cgggcacttt caccgcatct gcaaattggc ggtacatgtt caaatcggtc atggcttcag
     5581 ggaaaatcat gtccgcaccg gcttcgacac aagcttgggc gcgttcgata gcggcatcca
     5641 aaccttctac cgccagcgca tcggtacgcg ccataatcac gaagttctca tcaacgcgcg
     5701 catctacggc agctttgata cggtcgacca tttcatcttt agatacaatg gctttgttcg
     5761 gacggtggcc gcagcgtttt tgcgctacct gatcttcgat gtgaaccgct gcaacaccgg
     5821 cgcgttcaaa gttgcgaatg gtacgggcaa tattgaatgc accgccccaa cccacatcga
     5881 tgtccaccag cagaggcgta tccacgttgt ccgtaatgcg tcgtgcgtcg atcagcacat
     5941 cttccattgt ggtaatgccc aaatcaggga taccgcaaga acaggctgcc acgccgccgc
     6001 cggacagata gatggctttg aaaccgcttt gggtggccaa tcgtgcaaaa taagcattga
     6061 cgcaaccggc gacggcaagc ggattcgatt ctttcacggc ttggcggaaa cgtgctccgg
     6121 cagagtgttg actcatcata tttctccttt atagactttt tttcagtatg gacaggcttc
     6181 catcacattc ggacggcaaa acacagccat ccgacgcgtc gggcatccca atccaatcat
     6241 taaaaatata tgggaaaaat tatcttattg atatttaaaa cgaatcaaag aaaaacagca
     6301 gaccgttcgg aattatgcgg caaaaccgca gacaagaaga aaacaagggg attattcaga
     6361 aaaggggaaa acatctgaat tggtttcata gtaatgttcc tttgtagtgt tatgtagttt
     6421 tatttttcga taatctaaaa cacaagactg aaaatgtcaa acatttttaa ggagactcat
     6481 tacataattt taatatttta ttttaattcc atgattaaac tagattaatt ttgaattatt
     6541 tgtttcaata aaattgtcag gaaatcaaga aaaatttttg ataatcaaaa tcctgaaaac
     6601 ttataaaata ttttatacat aaaaacaatg tattattttt aagcaatccg atgtatttta
     6661 ttatgtattt gatttttata taatttaata aaaattaaat atatcggcaa acaaaatgcc
     6721 gtctgaaaac ctttcagacg gcatttttaa tatcgaatca atcagcccaa ataccagaat
     6781 ttcagtgccc acaatacggc aacaatccat accataggcg gaacgtcttt ggtgcggcgg
     6841 cataaaagtt taaccacggc ataactgatg aagccgaaag cgatgccgtc tgcaatcgaa
     6901 taagtaaacg gcatgaaaac aatggtcagg aacgcaggtg cggcttccgt catatcgtcc
     6961 caatcaatat ccctcgcact gcggagcatc tgcgtgccga cataaagcag ggcgggcgcg
     7021 gtggcaaaag cgggaacact tttcgccaaa ggtgaaaaca tcaggcaggc gagcatcaat
     7081 acgccgacgg taaccgccgt caggccggtc cgtccgcctg ccgatacgcc cgccgcgctt
     7141 tccacataag gcgtggtgga agaagtaccc aaagccgcac ctgccacaat ggcggtagag
     7201 tctgcaagca gtgcgcgttt caggcggggc agcttaccgt ccaccagcag cccggcacgg
     7261 tgggatatgc cgaccagcgt tccggtactg tcaaatagat cgaccaagaa gaagacgaaa
     7321 atcacactga ccatgctgac ggtaaacagg ccttcaaaat ccatctgcat aaaagtcggc
     7381 gcaatgctcg gtacttcgcc gatgatgccg tgaaattcat tcaaacccat caggctggca
     7441 atgacggtaa tggtcaagat ggtgatgatg attgcgcctt gaacgcggaa atgtcccaat
     7501 acgaccacca tagcaaaacc gaacaatgcc aacaacgcgg acggctgatg aatatcgccc
     7561 aaaccgacca aggttgccgg attggcaacg ataatgcctg cgcctttcag ggaaatcagt
     7621 gccaaaaaca aaccgatacc ggcagcaatc gacattttca aacccatagg cagtgcgttg
     7681 accagcattt ccctgacttt aaaaaagctg aacaggataa aaatcagacc ggagatgaac
     7741 accgcaccca acgcaacctg ccaaggcacg cccataccct taacgacggc aaaggtgaaa
     7801 taggcattca gccccatccc cggtgcgagt gcaatcggat agttgccgac aaaacccata
     7861 acaaaacagc cgatggcaga cgcgatacag gtagcgacga ataccgcccc catatccatg
     7921 ccggtctcgc ccaaaatcag agggttgacg ataacgatgt agcacatcgt caaaaaagtt
     7981 gtcaaacccg ccatcaactc ggtacgcacc gtcgtaccgt ttgccttcag cttaaaaatc
     8041 ccgtccaaca gtgtttgttt tgaagtgtcc atatctgaaa gtctgtatca agtggaaaaa
     8101 tctgaatccg ccgaccgcaa accgtacgaa attgcaacat cttgaaaaac atacctccca
     8161 aaccggacaa accgattcgg gaggcagcag gtttacgggc aaacagcaca ttatttgtct
     8221 gaattgtgtt gacgacgcaa aatcgcaggg atttcaaagt cgtcaagtac ggactgattg
     8281 tcgaaatccg cagcggtaag gttcatcgtg cggataccgc gattggtgcg gatcatacct
     8341 tcgacattgt ggctttgctc ctgtttggac ggagcaaccg cttctacctc ccttgccgga
     8401 acaaaatcga ccgcgccttt ttctttcaga ccggtagcga taatggtaat ccggatggca
     8461 tcttcgctca tggtctcgtc ttcagccgca ccgaatttgc attccaaatc gggatgcgcg
     8521 ctttggttga cgattttcat gacttcggac aactcggaca ttttcaagca acccggagca
     8581 gtcgtaatat tgaccagcac accgcgcgct ccgtccaagg ttacatcgtc cagcagcgga
     8641 ctggaaatgg cctggtcggt cgccatacgc gcacggtcga taccttgggc ataacccgaa
     8701 cccatcatag cgataccgcg gttgctcatc acggttttca cgtcggcaaa gtcgaggttg
     8761 atgatttcgc tcgggcaagt taccacttcg gaaatgcctg cgaccgcatc gcgcaataca
     8821 ttgtcggcgg cacggaaggc ttcgcgcatc gttacgtctt cacccaatgc agtcatcagt
     8881 ttgtcgttcg ggatgataat cagcgaatcg acgtgttctt tcaactgttc caaccctgcc
     8941 tgtgcgacat ggacgcgctt accttcatat gcgaacggtc gggtaaccac ggcaacggtc
     9001 agaatgccca aagacttggc aatctcagca acaaccggcg cggaaccggt accggtaccg
     9061 ccgcccatac cggtcgtgat aaacagcata ttcgcaccgc gaatggcttc ttcaatggct
     9121 tcccggtctt cctgggctgc cgcacggccg atatcgggat tcgcgcccgc gcccaaaccg
     9181 cgtgtcagat tcgtacccaa ctggattctc ttcgccgcat ggttttttgc cagagactgc
     9241 gcatccgtat tggcactgat aaactccaca ccgcgcacat tgttggcaac catgttattg
     9301 attgcattgc aaccgccgcc gcccaagccg attactttaa tcaccgcagg gctgactgcc
     9361 gattctgcca cgtcgtaaac aaattccatt caaaaactcc tgctcgcccc attcagagga
     9421 cggtttaaat aaattattat tcattatata agaagtatct tgctgctggc aaaatacttc
     9481 tcacctgtca aacggcaatc cacctgttca gaagctgttt tcaatccacc gtttcaatct
     9541 tgccaacaaa ccgccgcccc cttccctctc ttgcactgca ccgttttccg gctgcggcaa
     9601 gtttccttcc agcttgcatg ctgcatgaag cagcccgata gcggtagaaa aacgcggtgt
     9661 gcggacgcgg tcggacaaac cgcccatttc ttggggtgca ccggtgcgta caggcaaatc
     9721 gaagattttt tcggcaaatt ccacaatccc ggtcatcatg gacacaccgc cggtcagaac
     9781 gatacccgca ttcagcactt ctttggggaa acccgatttt tgcagctcgc ccagcactac
     9841 gccaaaaatc tcctgaatcc gtgcactgat gattgctgcc agaaccttac tggaaacctg
     9901 acgcgatgtc cggtcaccca cgcccggaac ttcaatcatc tcacccaagc cttccgtatc
     9961 gcatgatgcc acgccataat ggattttaat gtactcggcg gcatcgagag gtgttctcaa
    10021 cgatttggac aaatctttgg taatcagatt accaccggcc ggaatgacgg acgtatggcg
    10081 gatggcaccg ttcatataaa cggcaatatc ggtcgttccg ccaccaatgt cgatgacgca
    10141 tacgccgagg tctttttcat cttcagtcag caccgcctgc ccgcttgcca acggctgaag
    10201 catgatctga tcgcttttca aaccgcaccg ctcgatacat ttttggacat tctgcactgc
    10261 cgtacttgca ccggtaatga tgtgcacccg cgtatccaga cgcacaccgc tcataccgat
    10321 gggctccctc acgccaagtt gggtgtcaat aatgtagtct tgaaccacgg catcgagaat
    10381 tttttgatcg ggcgggatat tgattgcctt tgccgtttca atggcgcgat cgatgtctgc
    10441 ctgcgtgact tccccatctt taattttaac cacaccttgc gaattgagac tgcggatgtg
    10501 gttgcctgcg atacctgtgg taacgtgagt aattttggta tccgccatca gctcggcatc
    10561 attgaccgcc tgcctgatgg cttggacggt ggcatcgata ttggttacca tgcccgcgcg
    10621 caagccccgt gaaggagcct gccccaaacc gacgatgttg attttgtcgt catcttgaac
    10681 ttccccgatc agtgcgagga ctttagacgt accgatatcc agtacgctga tgtatctttg
    10741 ctgctgttcc attgttcgtc tgctcttaaa actgattgaa atttgcgtcg caccgtttca
    10801 gacggcacgg ccgtaatctg tccgatacct gttcccacta ttcttcggat tctttttcgg
    10861 gtaaaccgtc ggaagcatag cggactgaaa atccgtcctt atacctcata tccacatagg
    10921 ataaccgatt tttattttta cgcaacagat gctgccacgc ttcggtaaaa agccggaggc
    10981 gtttcatctc gttttcccgt ccgagcctga cggtgatgcc gttgtccaaa acgacaatcc
    11041 acgccgaacg tgccgtatag gtcatctctt tgatgcccaa accctgtttt gccaaaacag
    11101 tcgaaaattc gtcataacgg cggagcattt cggcagacgt tccttccgcg cctctgaata
    11161 ccggcattcc gggtctgtcc aagcgggctt caaaaacatt gccttcgccg tccaccaagg
    11221 catggtcgcc ccaacgcgcg accggcttgc gctcggtcag gacgacctca accgtgtcgg
    11281 gaaaacggcg gcgcaccatg accgacgcaa tccacggata ccggcggtag gcctcctgtg
    11341 cgccattgat gtccgtcctc aaaatattcc catggatgta ttctttcgcc aaactgccca
    11401 atgtcttctt atcggaataa accaggttgc ccttcagcga cacctgcttg acgggcagat
    11461 gattcgaatt gtaaaaccaa accagcccgg acgcagcaag cagcatcgcc atcatgacaa
    11521 gcagccagcg cgtcagccgt tccatcgctt cggcattatc ccacatgtgc ggtcttcaaa
    11581 atttcaatac ataaatcggc aaaacccacg cccgtaacgg cagcggattt cggtactaaa
    11641 ctatggctcg tcataccggg cagggtgttg atttccaaca gatagagttt gccgtcggta
    11701 tctttgagga aatcgacgcg cacgcagcct tccgcaccga ttgcctgcgc gccgcgaacc
    11761 gccagttcgc gcatcaggct ttcttcggct tcggtcaaat cttccgaagg acattgataa
    11821 atggtgtcgt cgcggttgta cttggcttcg tagtcgtaaa actcggttgc gggaatgatg
    11881 tgtatgccgg gcagcccttt gccgttcagg acggggcagg aatattcgcc gccgccgata
    11941 aaacgttcgg caatgatttc gccctgaagg tgtttcaatt cttcgtaaac gcttttcaga
    12001 cggccttttc ctttgacttt taccacgcct acgctgctgc cttcggccgc cggtttcaca
    12061 aacatcggca ggcccaattt ttcttcgacg gcatcgaaat cagtgtcgtc gtgcaggacg
    12121 gcgaactcgg gaacgggcaa tcccaatgcc tgccaaatca gtttgcagcg gtatttgtcc
    12181 atgccgatgg cggatgcggc gacaccgctg ccggtatagg gaatgcccaa cagttccaat
    12241 gcaccctgaa ccgccccgtc ttcgccgtaa gtaccgtgaa ggatgttgaa tgccgtctga
    12301 aaaccttgtg ccttcaattc agacaatggg gtttctttag gatcgaaggc gtatgcgtct
    12361 atgcctttgc tttttaaagc attcaaaatg gcggtgccgc tgtccagcga gatttctcgt
    12421 tcgctggaaa aaccgcccat caatacggcc actttgccaa aattctgcat tgtttttgtt
    12481 ctttcctgat tgctttatgc ttgttgccag aggtcgtctg aaacctgatt tgcggtttca
    12541 gacgatcttt atatgatgtt ccgtctgtca ggcgggtgtg cctcaaatct gtttcgacaa
    12601 tgccagcagc gcggcgggga cgcggttgat gcttcccgcg cccatattca acacgatgtc
    12661 gccgtcctgc aaaacgttca acagcatttc gggcagatcg gcaacgtttt cgcagtaaat
    12721 cggctcgagt ttgcccaaca cgcggatggc gcgggcaaga gcgcgggaat cggcggcggc
    12781 aatcggctct tcaccggcgg cataaacttc ggtcagcacc agcgcgtcaa cggtattgag
    12841 gactttggta aagtcttcaa acaaatcgcg cgtgcgggta tagcggtgcg gctggaaggc
    12901 gagtaccaaa cgtttttcca gatacgcgcc gcgtgcggcg gcaagggtcg ccgccatttc
    12961 gacggggtgg tgtccgtagt cgtccaccaa gagcgcggtc ccgccgtttg gcaacttgat
    13021 gtcgccgtat ttttggaagc ggcggccgac gccttcaaag ccgagcaagc ctttttggat
    13081 cgcttcaacc gatgcgccga cttccagcgc cacgccgatg gctgccaatg cgttcagcac
    13141 gttgtgtctg ccgggcatat tcagcacgac ttcaaacgac ccctgctcat gtcctttcat
    13201 ttgaacatgg acggtgaatt tcatttgcgc gccgacgttt tcgatgtcgg tggcgtagat
    13261 gtcggcggta tcgtccaaac cgtaagtagc ataaggtttg ctcactttgg gcaaaatcgc
    13321 gcggacgtgt tcgctgtcaa tacacaaaaa ggctttgccg tagaagggca tacggtggat
    13381 gaaatcgata aacgcctgat gcagtttttc gacgctgtgc ccgtaggtat ccatatggtc
    13441 ttcgtcgata ttggtaacga cggacataat cggtgtcagg tgcagaaagg atgcatccga
    13501 ctcgtcggct tcggcaacga tgtattcgcc tttgcccaag cgggcgttgg tgcctgcggc
    13561 gttgagtttg ccgccgataa cgaaagtcgg gtcaagtcct gccgcgccga ggatggaggc
    13621 ggtcaggctg gtggtcgtgg ttttgccgtg cgtgccggca atggcgatgc cgtcacggaa
    13681 gcgcatcaac tccgccaaca tcagggcgcg cggaataacg ggaatttgct gctccaacgc
    13741 agcgacaact tcgggatttt cttttttgac ggcggtagag gtaacgacga catccgcacc
    13801 gttaacgtgt tcggcggtat ggccgggata aacttgaatg cccaggctgc ccaaatgctc
    13861 ggtagcggca tttcgcgcct gatccgaacc ggaaacttta aagcccaaat tgtgcaagac
    13921 ttcggcgatg ccgctcatgc cgacgccgcc gataccgaca aaatggatgt tggtaactcg
    13981 atttttcatc ataatgttgc gttccggtgg attttcgatg cgtaaaggcg ttattttaaa
    14041 gggctgaccg tttgcgcgcc atagttttct gacaaatata tagcggattg aaataaaaaa
    14101 catccatgcc gtctgaacgg cttttcagac ggcatggttc ggcagtttac gccgcacacg
    14161 caatcgcggc ttccgccacg tcgtccgcac tgtgcggcag tgccaacgta cgggcgtttt
    14221 ctgcccattt gaggcatttt tcgcggttta agccgccgag aatctcggcg agtttttccg
    14281 ccgtcaactg ggtttgcggc aacagcaatc ccgcctccgc ctgcaccata aaacgcgcgt
    14341 tggcggtttg gtgatcgtca accgcgtgag gatacggcac taacaacgca cccaatcccg
    14401 ccgccgtcaa ctcggcaatc gtcagcgcgc cggcacggca aatcaccaaa tcggcatcgc
    14461 ggtaggcgga caccatgtcg gtaataaatt ccacgcattc ggctttcacg cccagcgcgt
    14521 cgtaatccgc ctgcaagctg cccagcttgc cccgtcccga ttggtggtac atctgcggac
    14581 gcgcattgtc gggcagcaaa gccaatgcct gcggtacggt tttgttcaaa acgtccgcgc
    14641 ccaaactgcc gccgaccacc aaaattttca gacggccttc acgcccttgg aagcgttcgg
    14701 caggcacggg caggttgcta atatcggcgc ggacggggtt gccgaccaag ccgccttcgt
    14761 ggctgaacgc tttcggaaaa gcgtacaaca cccgcttcgc ccagcgcgac aggtggcggt
    14821 tggacaaacc tgccacggcg ttttgctcgt gaatcacaat cggcacgcct aatagcttcg
    14881 ccgccaaacc gccggggaag gtaacgaagc cgccgaagcc gatgacgcac tcgacacggt
    14941 gtttgcggat aatccgctgc gcttcgcgga cggtttgata caaagtaacc ggcagcatca
    15001 gtttgcgttt gatgccgttg ccgcgcacgc ctttaatcgc cagcgtttcc aagcgtatgc
    15061 cgtattgcgg cacgatacgc tcttccatcg aatccttgct gcccagccaa atcacatgat
    15121 ggccgcgcgc gcgcaatgaa tccgccaccg ccagcgcggg gaaaatatgt ccgcccgttc
    15181 cgcccgccat cagcataaag gttttaccgc ccatgattta ctccacccga taaccgcgca
    15241 ttttccggcg gttttcataa tctatacgca acagcagcat catgctgatc agcatgaaaa
    15301 agactgacga accgccatag gacatcaacg gcagcgtcag acctttggtc ggcaaagcac
    15361 cgatgttcac accgatattg aagaaacttt ggataccgat ccaaatgccg atacccgaag
    15421 cgatataggc gttgaaagtc aaacccaaat cgcgcgactg cttgccgatg gaaaacgccc
    15481 gcaccaccag ccagccgtaa cagaatatca gcacgcacat accgaagaaa ccgaattctt
    15541 cggcgatgat ggcaaaaata aaatcggtat gcgcttccgg cagaaagccg cgtttgctca
    15601 aactcgcacc caaacccata ccgaaccact ctccgcgccc gattgccatc agagagtggg
    15661 taagctggta gccggcaccc tgcgggtctt tccacgggtc caaaaatgcc actacccgct
    15721 gcacacggta gggagcggcg gtaatcatca gcaccatccc gcccaagacg ctgcctacca
    15781 ggacgaaaaa atatttccac ggcaatcctg ccaaaaacag cattccaacg gcaatgacgg
    15841 taatgacgac aaacgaaccg aaatccggct gtaccattat cagcaccaaa ccgaacgcca
    15901 ccagcataat cggcaggatg atcgcccgga aacggccgta catttctaat gtttcacgac
    15961 gtgcctgcgg attggtggcg gacatgatca gattggccgt cccccgccaa atcgactgcc
    16021 aacccaaact ttccatgctg cgcaacactt cttcacggcg cgtgaacagg cttgccaaat
    16081 aaaggatgac cgccagcttg aacagctcgg tcggctggaa attcaacgga cccaaaggta
    16141 tccaacgggt cgcgccattg atttcgcgcc cggcaatcaa tacgactacc agcaacaggc
    16201 cggatagggc aaaaatccac ggcacaagcc gccgccatgt cctcatcctg caaagaaacc
    16261 ataacaaacc gctcgctatc aagccggcaa cgacgaaccc cgcctgtctg gtcaaataga
    16321 aaaactgatc gccgccttct tttgatgcca aatacacaga agccgaataa atcatcagca
    16381 ggctgaacgc cgtcatcagc accaccatcc acaaaagcgg cgcgtcgaat ttcctgccgt
    16441 cgcgcacaat cggcctgtcg agcagcagag tgtggacacc gtcgcccact tttaccaata
    16501 cttccgaaat cttcaaaaaa accacctgcc agtctgtttg cacctgccgc aaagggcaaa
    16561 aatttcagac ggcagacaat gccgtccgaa catacgatac atcccaaatc ggtattctaa
    16621 atctttactt gccgcccaac aatgacggcg tttgcatttc agacggcatc acaaagcctt
    16681 aaacgcttcg ataaacactt ccgaacggtg cgcgtagcct ttgaacatat caaagctcgc
    16741 gcaggcgggg ctgagcaaca caatatcgcc tgcttcggct tgggcatatg ccgtctgaac
    16801 ggcttctccc aaagtggcgc agtcggtcat attcaagccg cagccgtcca aatcgcggcg
    16861 gatttgcggc gcatcgacac caatcaagaa cacgcctttt gccttgccta ccagtgcatc
    16921 gcgcaggggc gtgaagtcct gccctttacc catgccgccc aaaatcacga agagcggatt
    16981 ttgcaaaccg gcaatcgcgg cggcagtcgc gccgacattc gtgcctttgc tgtcgtcgat
    17041 aaacaccacg ccgtttttct cgccgatttt ttccacgcgg tgcggcaggc cttggaaggt
    17101 tttgacgtgt tcgagcaatg cttcgcgcga caaaccgatg gcctcacaca aagccacggc
    17161 agccatgacg ttagcggcgt tgtgcagacc ttgcaacgga atgtcttgcg tgacaatcaa
    17221 atcttcattg ccttgtttca ggcggcctgt ctcgcgttcc aaccagaaat cagcttcgtg
    17281 ttccaacgaa aaccatttta cctcgcgccc ggcacgcttc atcgcgcggc agaacgcatc
    17341 gtccgcattc aaaacctgca cgccgtcgcc acggaaaatc ttggctttgg tatgcgcata
    17401 gtcgagcaag tcgtcgtagc ggtcgagatg gtcttcggaa atgttcagca ccgtcgccgc
    17461 agtcggacgc aggctttcgg tgttttccag ttggaagctg gaaagctcca acacccacac
    17521 gtccgccttt ttgccttcgc gctgccattc cgcctccaaa accggcgtgc cgatattgcc
    17581 cgcgataacg gtatccagcc cgcacttgat acagagatag ccgaccaggc tcgttaccgt
    17641 ggttttgccg ttgctgccgg taatcgcaat taccttgtcg tcccggcggt tcacaatgtc
    17701 cgccagcaat tcgatgtcgc ccaacacgcg tccgccgttt tgcttgaacg cctcaatatc
    17761 cgactgccgc tcgctgatgc cgggactgag agccagaata tcgaaaccgt tgtccagcgc
    17821 atctttcaga cggcccgtgt aaaacaccaa cccgtcaaac atcttaccga tttgcgacac
    17881 gcgttccggc ttcagctccg catcatacgc agcaacctcc gcgccgtttt tgcgcaggta
    17941 ggcaatcatg gaaatacccg taccgccgag tccggcgacg aggatttttt tgttttgaaa
    18001 agtcattttg gtttgtccta aaacaaatca tattgagcag gagatgtccg cccctgccca
    18061 agccgctttc agacggcatc gcgagctgtt caataacccg ccttcaggcg ttggtcattg
    18121 tcgcagccgt cttggtctcc gttttgacaa gccttgccaa accattcttg tgcaagggcg
    18181 cggtcttggc gcacgccgcg tctttcggca tacatcacgc ccaaattgtt ttgggcttgg
    18241 gctaccccct gcgctgccgc ctgccgaaac catctgaccg cttcgacatc gtcttggcgc
    18301 actccacgtc cttcggcata tatcacgccc aaattgtatt gggcttggac aaccccctgc
    18361 gctgccgcct gccgatacca tctgaccgct tcggtatcat cttggcgcac gccgcgcccg
    18421 ttggcataca tccagcccaa attgtattgg gcttgggcta acccctgttc cgccgcctgc
    18481 cgataccatc tgaccgcttc agcatcatcc cggcgcacgc cgcgtccttt gtaatacatt
    18541 gcgcccaaat tgtattgggc tgctgcattt ccctgtgctg ccgcctgcaa gttttcccga
    18601 aaatccgata cgtcatccgc ccacaccgct cggttcaagc ccaaggcaat cagggcggcg
    18661 gcaagccatt tgactgtctg tttcatggtt ttacttctgt tttagtataa ggcgggtttc
    18721 agccaccgtt aacgataggg ctgggcggat tgtcgccgca ggtttattgc gcgttcaaat
    18781 gccgtctgaa agatgttcag acggcatagg ttcagcggat tttgagggta ctcaaaccga
    18841 tcaacaccaa gacgatggta ataatccaaa agcggacgac gacttgggtt tctttccagc
    18901 ctttttgttc gtagtggtga tggatgggcg ccatcaggaa gatgcgtttt ttggttttct
    18961 tataccagcc aacctgaagc ataacggata cggcttctac gacaaataat ccgcccataa
    19021 tgacgaggac aaactcttgg cggacgataa cggcgacggt accgagcgcg gcacccaatg
    19081 ccaatgcacc gacatcgccc ataaagactt gcgcgggata ggcgttaaac cacaagaaac
    19141 cgaggcacgc gccgcacatg gcggtacaga aaatcaccac ttcgtttgcg ccggcaacgt
    19201 aaggtaattg caggtattgg gcaaattgtg agtggccgct ggcataggcg aagatggcga
    19261 ggccggcggc aacgaggacg acggggaagg tcgcaaggcc gtccaagccg tcggtgaggt
    19321 tgacggcatt ggatgtgccg acgatggtca ggtaagacaa caccaaaaag ccgaccacgc
    19381 ccagcggcag ggcgatttgt ttgaagaacg ggacaatcaa aatattgttg gcggaattgg
    19441 cggcaaggta aaacaatgcc aaactggcga taatggcaac gcttgactgc cacaccattt
    19501 tgaatttggc ggacacgccg ttggggtctt tatagacgac tttgcgccag tcgtcgtaaa
    19561 aaccgagtgc gcccgtggcg agcaatacgc ccaagagaat ccagatatac gggtttgccc
    19621 agttgcccca caacagggtg gacacggtaa tggcggtcag aatcagcgaa ccgcccatcg
    19681 tcggcgtgcc gtttttgacg aggtgggttt gcggaccgtc ggtacgcact gcctgcccgc
    19741 atttgagcgc ggtcagcctg cgtatcgtcc acgggccgaa catcagggaa aacgctaagg
    19801 cggtcaacgc cgccatgacg gcgcggaatg tggtgtattg aaaaatattc agaccggtta
    19861 accagttgct gaaatgtgcg agccataaaa acatggggct tccttttttt gttttgt
//
LOCUS       DAWIVB010000018        19792 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWIVB010000018 DAWIVB010000000
VERSION     DAWIVB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.018"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(203..278)
                     /locus_tag="WIX60_000451"
     tRNA            complement(203..278)
                     /locus_tag="WIX60_000451"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="WIX60_000452"
     CDS             complement(371..730)
                     /locus_tag="WIX60_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ5304768.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="WIX60_000453"
     CDS             complement(753..1397)
                     /locus_tag="WIX60_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ5304769.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="WIX60_000454"
     CDS             complement(1399..1935)
                     /locus_tag="WIX60_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ5304770.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="WIX60_000455"
     CDS             complement(1947..3044)
                     /locus_tag="WIX60_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ5304771.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="WIX60_000456"
     CDS             complement(3047..4483)
                     /locus_tag="WIX60_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ5304772.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLILSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIX60_000457"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIX60_000457"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ5304773.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMTHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCNVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFTVILDFDTPSDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="WIX60_000458"
     CDS             complement(5473..6120)
                     /locus_tag="WIX60_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ5304774.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCT
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFGEIHQELIALFAMIGQDRR
                     TDLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDVGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIX60_000459"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIX60_000459"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ5304775.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPSTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            complement(7294..7431)
                     /locus_tag="WIX60_000460"
     CDS             complement(7294..7431)
                     /locus_tag="WIX60_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ5304776.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            7495..8571
                     /locus_tag="WIX60_000461"
     CDS             7495..8571
                     /locus_tag="WIX60_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ5304777.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTIIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8778..10244)
                     /locus_tag="WIX60_000462"
     CDS             complement(8778..10244)
                     /locus_tag="WIX60_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ5304778.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WIX60_000463"
     CDS             complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WIX60_000463"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ5304779.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WIX60_000464"
     CDS             complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WIX60_000464"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ5304780.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGNRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13523..13807)
                     /locus_tag="WIX60_000465"
     CDS             complement(13523..13807)
                     /locus_tag="WIX60_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ5304781.1"
                     /translation="MKKLLLAAVVSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WIX60_000466"
     CDS             complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WIX60_000466"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ5304782.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAEFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVSNLSNGEDSGLQSPIVKYLSENALKEIIARTGAQ
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKATETKEV"
     gene            complement(15824..16525)
                     /locus_tag="WIX60_000467"
     CDS             complement(15824..16525)
                     /locus_tag="WIX60_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ5304783.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQPGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16668..17816)
                     /locus_tag="WIX60_000468"
     CDS             complement(16668..17816)
                     /locus_tag="WIX60_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ5304784.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="WIX60_000469"
     CDS             complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="WIX60_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ5304785.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18543..19685
                     /locus_tag="WIX60_000470"
     CDS             18543..19685
                     /locus_tag="WIX60_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5304786.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5088 a         5105 c         4611 g         4988 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gctgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaac aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caagtaagca gcttctcact gctgaccatt
     1261 tcgtccgcat ctgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgctg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttaatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ttcatggtaa cccaagctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgtg ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcggctt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaagaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggtgtctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcatca
     4441 gcaataccgc cagtgccaaa ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg cgcactttgc ccgacggcat accgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatctg acggcgtatc aaagtctagt attaccgtaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agcctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgttt tcaagcctgt tttgcaatgg gtgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacattg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg tcattaaatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgacg tcaatttccc gaacggtgca acgcggtgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgga taagatctgt
     5641 acgtctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgccaaa
     5701 cgggcgtgcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggtgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgtcc gccttgatga ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcacg ccgcaaactg ccgcacttca tccgttgtca tatctgccgt
     6181 taaagacagg cggattctgg atgtgttttt gggtactgtc gacggtctga tgggcaggca
     6241 ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc aaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacggaaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgcg gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacacca tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggcataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcgggtctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca
     8161 cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg caccggatcg
     8221 gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa atcgctggcg
     8281 tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac tgaggaaacc
     8341 aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag
     8401 gcacaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac cgaaccggct
     8461 gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc ggttgatgta
     8521 ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg aataaccttc
     8581 tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt tgtttggctt
     8641 ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat gccatctgaa
     8701 ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac agattggaaa
     8761 tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct gccgataacg
     8821 aaaaaaatca gaaaattgac tacgctcatg ataaaacccg ccttccacca ttctcccatt
     8881 gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg agtgagggtc
     8941 atcataatgt tggatgcggc cgccatcatc agcgcggtcg gcatcgccgg ggcattcagt
     9001 gaaacggcag cagcgaaaaa tgcgccgaac atagcggtaa tatgtgcagt agtactggca
     9061 aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc cgtgccgcta
     9121 acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat cagtccgagt
     9181 ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt atcccacgcg
     9241 cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag caggcttaat
     9301 ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt aataagggcg
     9361 ggaacatctg cccacaacag cagcaagata ccgaaaatga ccgccataat gatttcgtct
     9421 gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc attgggcgtt
     9481 tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa aaaggcgata
     9541 acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa actactgcct
     9601 aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt aataaacata
     9661 gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc catcttgcct
     9721 tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt
     9781 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag cagttcggaa
     9841 agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat aaacaaatat
     9901 ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac tgcgatggca
     9961 atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc cgcgcccggt
    10021 ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcgccc
    10081 aacggcatgg ccttgccgat aatggcggca atcacaccga caaacatggc cagcagcgtc
    10141 caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccagggc gcacaatact
    10201 gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac ctccgtaaaa
    10261 aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata agatatattc
    10321 tgatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca gtatttgaat
    10381 tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg atgccggacg
    10441 ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg gcaccggcca
    10501 aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc ataccgacga
    10561 tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc aagccgccgc
    10621 atacgggcgt accggtccca ggggcgaacg acgggtccag gcagtctatg tcgaaagtca
    10681 ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct tcaacactgt
    10741 cttcattgac tttaggggcg gacaacacag taaaaggcaa ttttttactg tgttcggtgc
    10801 gtatgccgat ttgtacggaa cgggacgggt cgatgaggcc ttccttgggg gcggtataga
    10861 acatcgtacc gtggtcgtat tcgctgccgt tgtcgtaggt gtcggtgtgc gcgtcaaaat
    10921 gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggt agggtaatga
    10981 aatggtcgcc gcccaaactc aaacagcgtt tgccggaaga aagtaatttg ccggcgtgcg
    11041 cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaag tcgccgcaat
    11101 caataatgtt caggcgttcg cgcacatcaa atgtccatgg aaacctgcgg tgctcccaag
    11161 cgaggttgac ggaggcgcgc cggatggctt caggaccgaa acgcgcgccg gaacgccctg
    11221 aaaccgccat atcataaggc acgccggtaa taacccaatc ggcatgactt tcatacggca
    11281 taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagggag ttgtcggttt
    11341 gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt caatcggtcg
    11401 tgatgattgg tgtttgagta agaaaatcgg gcttcagacg acatatccga tgccttgatg
    11461 cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt cttttaagaa
    11521 agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat agcgtttcat
    11581 cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt ttccttcatc
    11641 gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcggcag tggcagtgtc
    11701 gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca taaaaaagcc
    11761 taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg cgatgccgtc
    11821 tccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc
    11881 gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa gttgatctat
    11941 gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct tatcggcaaa
    12001 acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat taaacaattc
    12061 gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact
    12121 caacagcccg acattatact gattatgcac atcagcaaga tcaaactgcc cttcgtgtat
    12181 ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag tttcccacat
    12241 actgtgcaac acacgcggtg cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg
    12301 ttcaacgcct ataacattag caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct
    12361 ctcggtgatg attgtcggat gcggcagccc gtgttcgaga caagcctgac tgatgcccca
    12421 tacgactgtg gcggcatatt cgttgaggct gtaattaacg gaacaatccg attgtgtgcg
    12481 gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga tatttacccc
    12541 cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg caacatcacg
    12601 gatgttccca agctgcgagc ccaaatggaa atgcaaaagc tgcaggcaat ccagcctgtt
    12661 tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc cgaattttga
    12721 tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc tggcgcgcac
    12781 acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt gtatttcgga
    12841 cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca aggcgaaacg
    12901 gatatattca cggtctttat agccgttgca gacgattaat gtttgccggt tgccggcgtg
    12961 tgccaaaacc gccatcagtt cggctttaga accagcttcc aaaccatgcg gttgtccgct
    13021 tgacataagc gattcgatga cgcgacggtg ttggttgacc ttgatagggt aaaccaaaca
    13081 ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt taatgtcgcg
    13141 gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt
    13201 ttgttgcacg gcttcagtca gtttttgcag tgaaacagtt tgattgtgtt gcgagggatt
    13261 ggggcggacg atgatttcgc cggaatcgtc aacatcataa taacctatgc cccaatgatt
    13321 aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc gtctgtcgtg
    13381 ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat aagaaaagcc
    13441 tgcctcatac ctgatgagga acaggcaaaa tgccgtctga acgcttcaga cggcattttg
    13501 gttcatcttt ccatcaaagg agtcagcgat cgagctgctc tttgatgatt ttcaggtcgg
    13561 ggtaagacaa cacgatgtcg tattcgcggc cgtctttata ggcttccact tccagcacag
    13621 gcttacccca atggtcgtcg gcatcgacat cgtaaacctg ataaccgcgc tgctccaaca
    13681 ttttcacagc ttttgtgcgg ttttgttcaa aatggggatc gccgtaaatc tgacgctcgg
    13741 cagagtcgcc ggcaaatgcg gcagcggcac tcagagaaac aacggcagcc aataacagtt
    13801 ttttcatttt cagtcctttt tttatcggtt gattgaacaa gatgtgtttt tcaataccgc
    13861 cattaaaaca cagcaaaatt aggtttgaat tagagggaag tcaagaagcg taaatgccgt
    13921 ctgaaaaaaa cagagccgtc aaacggctcc gttttcctta tacttcttta gtttcggttg
    13981 ccttctggcg caaacgcaaa cttaattcac gcaactgctt gtcgtccacg ctgttgggcg
    14041 cgttggtcag caggcattgg gcgcgttgtg ttttcgggaa ggcaatcacg tcgcggatgg
    14101 attcggcacc ggtcatcagc gttaccagac ggtcgaggcc gaatgcaaga ccgccgtgag
    14161 gaggtgcgcc gaatttcagg ttgtccaaga ggaagccgaa tttctcttgt tgctcttcag
    14221 ggctgatttt cagcgcggca aacactttct cttgtacgtc tgcgcggtga atacggatag
    14281 agccgccgcc gatttcccag ccgttcaata ccatatcgta ggcgcgtgcc aaacaatttg
    14341 ccgggtcgga aaccatcagg tcttcatgac cttcttttgg cgcggtaaac ggatggtgta
    14401 cggcaacgta gcggtcggct tcttcgtcgt attcgaacat tgggaaatca acgacccaca
    14461 aaggtttcca ttcgtctgtg aaatagccgt tgtctttgcc gtgctccaag ccgactttga
    14521 tacgcagtgc gccgatggct tcgttcacga ctttggcttt gtctgcgccg aagaagatga
    14581 tgtcgccgtt ttgcgcgccg gtacgcgcga taatttcttt cagggcgttt tcggacaggt
    14641 atttcacgat tggagattgc aggccgctgt cttcgccgtt ggaaaggttg ctgacatcgt
    14701 ttactttgat gtatgccaga cctttcgcgc cgtagatgcc gacaaatttg gtgtattcgt
    14761 cgatttcttt gcggctgaat tctgcgccgt tcggcacgcg cagagcgacc acgcggccgc
    14821 ctttcatgtc ggctgcgcca cggaagactt tgaattcttc cgttttcatc aggtcggtca
    14881 actcggtaaa tttcaagttg atgcgcatat ccggtttgtc agagccgtag tagaacatgg
    14941 cttcagagta aggcatgcgt gggaagtcgc ccaaatctac atttaaagca tctttgaaga
    15001 cttgtttggc catgccttca gtgatgtcca tgatttcatc ctcgtttaag aacgaggttt
    15061 ccaagtcgat ttgggtaaat tcgggctggc ggtcggcacg caggtcttcg tcgcggaagc
    15121 acttggtgat ttggtagtaa cggtcgaaac ccgccaccat caacagttgt ttgaataatt
    15181 gcggcgattg cggtagcgcg aaaaactcgc ccggatgaac gcggctcggc acgaggtagt
    15241 cgcgcgcgcc ttcaggcgtg gagcgggtca gcatcggggt ttcaatgtcg atgaaacctt
    15301 gcgcgtccaa gtagcggcga acgcccatag caacttggta acgcaggcgc aggttgcgtt
    15361 gcatcaccgg acggcgcaag tcgataacgc ggttggtcag gcgaacgttt tcgctgatgt
    15421 tttcatcgtc gatttggaac ggcggcgtgg cggcggcgtt caagacttcg atttctttgg
    15481 caaggatttc gattttgccg gaaatcattt tatcgttggt cgtgccttcg ggacggttgc
    15541 gtacgcggcc ggtaatgctc aaaacgtatt cgttgcggga ggaatcggca gcggcaaacg
    15601 cttcgggcgt gtcgggatcg atcacgactt ggacgatgcc ttcgcggtcg cgcaggtcga
    15661 taaaaatcac accgccgtgg tcgcgtcgac ggtgtaccca gcctttgacg gtaacggttt
    15721 ggtctaagta ttgctcactg atcaggccgc aatagttggt acgcataaaa tcacctttta
    15781 ttgatttaaa ctgaaaacag aaaatgccgt ctgaacggcg gctttattgt tgttcgggca
    15841 aatccgcctt ttcagacggc ataggtcctg ccaatgtttt gacgggcagg tcgtcaggga
    15901 tgaccatacc cagcgaaatc acatatttca atgcttcgtc cacgctcata tcgagttcgc
    15961 gcacatcgct tttctttacc ataatatagt aaccgccggt cggattcggc gtggtcggaa
    16021 catacacgga aagataatcg ccgtccttcg gcaatgcggc cttaaccgca ttcgacacct
    16081 gccctgacac gaaagcaatc gtccaaatac cgggctgggg aaacggcacg agtaccggcg
    16141 ttttaaacga acggctgctg tcggacagca gcgattcgga tacttttttc acactcgaat
    16201 agatggattt cacaaccgga atccgcccca acaggctgtc ccacgcggcg aggatctgcc
    16261 gacccaatac gttggcggca aacaatccgg ttacaaacaa tacggcaatg gcaacgataa
    16321 cgcccagccc cgggatatta aaccccaaaa catattgcgg ccgccattgc ttcggcagca
    16381 ggttgacgag ctgatcggac gcggaaacga tataggaaac cacccaaacc gttaccgcaa
    16441 tcggcagcca gaccaaaatg cccgtaatca gatatttttt taacgccttg gcagctttgc
    16501 cgccttcggc cgcaggttcc gtcatcttgc ttgattccga caaagtccgt acaaaccgca
    16561 cattatacgc gtttgcccgg attcaaacga aatttttatc ccgccccgcc aaaccgccgg
    16621 cggcttcaga cggcacggca acttgatata ccgtctgaac acgcggttca gatgccgtcc
    16681 aagtcgttga acatcaaccc gataccgata ccgttctgct tgtggttgta gtcgatcagg
    16741 ctctcgccgt aaccgtggaa tccgcgtacc acgcctttga gtttgccctt aatcggaaac
    16801 gtgtaggcgg cttcaatcgc gccgtagccc gttttggggt tgtagcgcaa tacggaatac
    16861 acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc catatagtcg
    16921 gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac ccacacgcgc
    16981 ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cgtaaatcct gttccacgaa
    17041 cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgcacc gagcatacgc
    17101 agcctgccgc cgaacggcaa atccgccttc acaggctggg tcaggaaaat ttcaggtttg
    17161 taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg ccaatcggat
    17221 ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc ggcaattttg
    17281 cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa tttttcctgt
    17341 acagtcgtac cgcgcgtcgg cgaacccggg gcatagttgg gcgaattgtt gtaccagagc
    17401 ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa atcgtttttg
    17461 tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc cgcactgtcg
    17521 gcaggaagcg catccccgcc tttttcaaca acaatgaccg cctcgccctt atccaggctg
    17581 ctgcggacgg tttccgtcag attgagtacg gctttcgact cctgcccttc ctgccctgcc
    17641 gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt aacattgtcc
    17701 gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg atgccatcgg caacagtcct
    17761 gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt cggcatattg
    17821 gttttcaaac ggcattttat agcggattcg gataaaaaat cgcacccttt ccgccatttc
    17881 gggattttgc ccccgcaata cagaaaaccc cgaaaccgtc gggcttcagg gttttccgct
    17941 tatcgcgtat caaccgcctt ggcggttttg caaaaatcaa gccaaggctt ttacttttgc
    18001 agacagacgg cttttgtggc gtgccgcttt gtttttgtgg aacacgccct tgtcggcgat
    18061 gcggtcgatg actttgacgg actcttggta aaccgcttgt gcggcagctt tatcgcctgc
    18121 ttcgactgct ttcaatactt ttttcactgc ggtgcggaat gcggtacgca ggctagcatt
    18181 gtgggcgcgt tgtttgacgg actggcgggc acgtttgcgt gcttgtgcgc tgtttgccat
    18241 attggaatct cctagaagaa aaataaattc tgtaaacgcg caattttaaa gggggtttac
    18301 attatatgca agccttttct ctctatccgc ccgtcggaca ccctgacagc gcaaaaaaac
    18361 ggcaaaatcg ggcggacgtt tttctcccct tttggaaaca ggataatttt ttaggaaaat
    18421 cagtccaata tcgctttgag tttgcacaaa tttactttac aatgcccaaa ccttgcgccg
    18481 gggtaacgat ggggcgcggg gatttctttt tttccaacct ctttcatcag gaatctgccc
    18541 gtatgaaaaa atccgtatta gccgttttgg ccgcgctgtc tctggcagcc tgcggcggca
    18601 gcgaaaaaaa cgccgtgcaa cctcaagcag acgctgcgtc tgctgccaat gccgaagccg
    18661 ctgccaccga tactctgaac atctacaact ggtcgaacta tgttgatgag agtacggtcg
    18721 aagactttaa aaaagccaac aatctgaagc tgacttacga cttgtacgaa aacaatgaaa
    18781 cgctggaagc caaaatgctg accggcaaat ccggttacga tttggtcgtg ccgggcatcg
    18841 ccttcctgcc gcgccaaatc gaggcgggcg cgtatcaaaa agtcaacaaa gacctgattc
    18901 ccaactataa aaacatcgat cccgaactct tgaaaatgct ggaaaccgcc gacccgggca
    18961 accagtatgc cgtcccctat ttctccggcg tgaacacgat tgcgattacg gcgaagggca
    19021 aagagctttt gggcggcaag ctgcccgaaa acggctggga tttgctgttc aaacccgaat
    19081 acacccacaa gctgaaatcc tgcggcatcg ccctgtggga caccccgagt gaaatgttcc
    19141 cgattttgct gaactacttg ggcaaagacc ccaaaggctc gaatcctgaa gacttgaagg
    19201 cggcggcgga agtgttgaag tctatccgtc cggatgtcaa acgtttcagc ccgtccatca
    19261 tcgacgagct ggcacgcggc gacatctgcc tggcggcagg caacggcggc gatttgaact
    19321 tggcgaaagc acgttccgag gaagtgaaaa acaacgtcgg catcgaagtg ctgacaccga
    19381 aaggtatggg cttctggatt gagtcttggc tgattcccgc cgatgcgaaa aacgtcgcca
    19441 atgcccacaa atacatcaac tacacgctcg accccgaaat cgcggcgaaa aacggcatcg
    19501 ccgtaacctt tgcccccgcc agcaaaccgg cgcgcgaaaa aatgcctgcc gagctggtga
    19561 acacccgttc catcttcccg aacgagcagg atatgaaaga cggtttcgtg atgccgcaaa
    19621 tgagcacgga tgcgaaaaaa ctgtctgtca gcctgtggca gaaaatcaaa gtcggcacca
    19681 actgatttga agcattaaaa atgccgtccg aacgatgttc ggacggcatt ttatattgga
    19741 ttgaaataga aatatttata tagtggatta acaaaaatca ggacaaggcg ac
//
LOCUS       DAWIVB010000019        19233 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWIVB010000019 DAWIVB010000000
VERSION     DAWIVB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.019"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(173..1948)
                     /gene="ptsP"
                     /locus_tag="WIX60_000471"
     CDS             complement(173..1948)
                     /gene="ptsP"
                     /locus_tag="WIX60_000471"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ5304787.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(1948..2217)
                     /locus_tag="WIX60_000472"
     CDS             complement(1948..2217)
                     /locus_tag="WIX60_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ5304788.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2286..2723)
                     /locus_tag="WIX60_000473"
     CDS             complement(2286..2723)
                     /locus_tag="WIX60_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ5304789.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DINNNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2805..3368)
                     /locus_tag="WIX60_000474"
     CDS             complement(2805..3368)
                     /locus_tag="WIX60_000474"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ5304790.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALGGK"
     gene            complement(3437..4261)
                     /locus_tag="WIX60_000475"
     CDS             complement(3437..4261)
                     /locus_tag="WIX60_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ5304791.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLMSRQGYAFAPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            4353..4985
                     /locus_tag="WIX60_000476"
     CDS             4353..4985
                     /locus_tag="WIX60_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ5304792.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5192..6976)
                     /gene="rmuC"
                     /locus_tag="WIX60_000477"
     CDS             complement(5192..6976)
                     /gene="rmuC"
                     /locus_tag="WIX60_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ5304793.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAHLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYAAEDEAV"
     gene            complement(7315..8100)
                     /locus_tag="WIX60_000478"
     CDS             complement(7315..8100)
                     /locus_tag="WIX60_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ5304794.1"
                     /translation="MKQTLKNWFAALLLAVPMSAAVASGGGHYEKVDIDLRDQVSLQH
                     GAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDAA
                     KWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQG
                     VQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVNY
                     LVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLNKEYWKDVH"
     gene            complement(8115..9464)
                     /locus_tag="WIX60_000479"
     CDS             complement(8115..9464)
                     /locus_tag="WIX60_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ5304795.1"
                     /translation="MANQTNSKAKALLGWVDARFPLSKMWKEHLSEYYAPKNFNFWYF
                     FGSLSMLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKLKDENGVPLDGIPFFPYYVVHDILAVTIFLIVFCAVMFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFAGTQVWGVIGMGA
                     AVVLIALLPWLDKGEVKSVRYRGPIFKTALVLFIIAFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDTNKPVPERVTMSTTKQKIMFFVYVGITVVGAYLFATNI"
     gene            complement(9483..10064)
                     /gene="petA"
                     /locus_tag="WIX60_000480"
     CDS             complement(9483..10064)
                     /gene="petA"
                     /locus_tag="WIX60_000480"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ5304796.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10187..10936)
                     /locus_tag="WIX60_000481"
     CDS             complement(10187..10936)
                     /locus_tag="WIX60_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ5304797.1"
                     /translation="MVLCRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GNPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11067..11996
                     /locus_tag="WIX60_000482"
     CDS             11067..11996
                     /locus_tag="WIX60_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ5304798.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12251..12643)
                     /gene="rpsI"
                     /locus_tag="WIX60_000483"
     CDS             complement(12251..12643)
                     /gene="rpsI"
                     /locus_tag="WIX60_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ5304799.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12653..13084)
                     /gene="rplM"
                     /locus_tag="WIX60_000484"
     CDS             complement(12653..13084)
                     /gene="rplM"
                     /locus_tag="WIX60_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ5304800.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13262..13441)
                     /gene="ssrS"
                     /locus_tag="WIX60_000485"
     ncRNA           complement(13262..13441)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WIX60_000485"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13447..13752)
                     /locus_tag="WIX60_000486"
     CDS             complement(13447..13752)
                     /locus_tag="WIX60_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ5304801.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13763..14092)
                     /locus_tag="WIX60_000487"
     CDS             complement(13763..14092)
                     /locus_tag="WIX60_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304802.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14147..15136)
                     /locus_tag="WIX60_000488"
     CDS             complement(14147..15136)
                     /locus_tag="WIX60_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ5304803.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15259..17961)
                     /gene="ppc"
                     /locus_tag="WIX60_000489"
     CDS             complement(15259..17961)
                     /gene="ppc"
                     /locus_tag="WIX60_000489"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ5304804.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPGVRVPDILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNGDVTALSDKSPDEEIARAEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKETGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18131..18901
                     /locus_tag="WIX60_000490"
     CDS             18131..18901
                     /locus_tag="WIX60_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ5304805.1"
                     /translation="MTTTEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCG
                     GLGAAALPYLAASGVGTLTIADSDTVELHNLQRQVAFDEGDVGKLKTEALAGRLKRIN
                     HTVNVRAVNEKLDGCRLTGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVR
                     FEGQLAVYRPDLPDSPCYACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILL
                     DAGEPSHGRLAVYRALEGGWQYFDLPRNPECPVCGTAR"
BASE COUNT         4122 a         5235 c         5087 g         4789 t
ORIGIN      
        1 acctagattc ccgcctgcgc gggaatgacg gattttaggt ttctgatttt ggttttctgt
       61 ttttgaggga atggccgatt ttgggtttct gtttcggttt tctattttgc aagaatggca
      121 aaatttcaga ttgcgggcat tgttaagtat ttctattttt tacctgccgt atttatttcc
      181 gccccttgaa gtcggcttct tcctcgacag acacgctgtt catctgtttg atcagctttt
      241 ccgacttctc ttcgtcttcg cagcggatga ctttcacaat atcgctttcg agctgtccga
      301 cattgctgtg cagaatgatg tttttgacgg gcaggatgtt gttggggttc atggaaaaac
      361 ggcgcagccc catacccaat aaaacgcggg taaacgcggt atcgcccgcc atctcgccgc
      421 atacggatac gtctttgtcc atgcggttgg cggtacggat gacgtgttgc agcattttca
      481 gcacggcggg atggccgggc tggtagaggt ggctgacgct gtcgtcgccg cgatcgacgg
      541 acaagatgta ttgaatcagg tcgttggtac cgacggagat gaaatcgacc agtttcaaaa
      601 tactgccgac ggtcagcgcg gcagacggaa tttcaatcat acagccgatg ccgactttac
      661 cgaaggcatc gccgcgttcg gcaagctggc gttgcgcggt gtcgaggtgg atgaggcact
      721 ggcgcacttc ggatacggag gtaatcatcg gccacatcat ccgcacgggg ccgtgtaccg
      781 ccgcacggag gatggcgcgc atctgggtgc ggaacatgac cggttcggca aggcacaggc
      841 ggatgccggt catgcccagc gcggggttga ggctgccgtt gggcgtgctg tttttcccga
      901 accagcgcgg gtttttgtcc acacctaaat cgactgtccg tatcgttacg cttttgcctt
      961 tcattttttt gacaatcgcg ctgtacactt cgtactgctc gtcttcagac ggcatcgtat
     1021 cgcggttcag gtaaagaaac tcgctgcgga acagcccgat gccgtctgcg ccgaggttgt
     1081 gcagcggttt cacgtcttcg gcggattcta tattgcccac aagctcgatg cagaccccgt
     1141 cggcggtggc ggcggcggtt tttttgagct tgttcaaatc gcgtttgtgg ctgcggtatt
     1201 cgcgggcacg gcggcggtat tcgttcaaca ccgactcatc cggcgcgata atcaacacgc
     1261 cgttgatacc gtccacaatg accgtttcgc cttcggtaat cagtttgcgc gcgttgtgca
     1321 gcccgacgac ggacgggatg tccaagctcc tgcccaaaat cgccgtatgc ccggtggggc
     1381 cgccggcatc ggtaacgaag gcggcaatgc gctgctcttt aaacaaaacc gtgtcggcgg
     1441 gcgaaaggtc gtttgcaatc agaacggttt cgtcaaacag gttgtcggca acttccaact
     1501 cgttgccctg cccgatcagg ttgttgtgga tgcggcggac gacttgcagc atatcctgct
     1561 tgcgttcgcg caaataggca tcgtccatat tgtcgaattg ggcggcgagt ttgtcgctct
     1621 gctgcttcaa tgcccactcg gcgttgattt tttgttccct taaaatatcg acgggttcgc
     1681 gcgacaaggt aacatcggtc aagagcatca ggtgtagcga gatgaacgcg cccaactcgg
     1741 tcggggcgtt ttcgggaatc gcgctgcgga gctgttccaa ctctttgcgc gtggctttga
     1801 cggcggcatc gaaacgttcg gcttcggcat cggtgtccgc ctccgcaaca tcatactgcg
     1861 gcacttcctc cgtaccgcgc gcaatcaggt gggcgcaacc gacggcaatg cctttgcccg
     1921 ccgccacgcc gtgcagcacg atactcatta ttcgccctcg ccgaagtagc cgttgattaa
     1981 gtcggtcagg gcgcgcatcg cttccgcctc gtccgcgccg tccgtctcca gttcgatgac
     2041 cgtacccttg gcggcggcga gcatcatcag ccccataatg cttttgccgt tgacgcggct
     2101 gtcgtttttc gtaacccaga cttcgctttt gaattgggac gcggtttggg tgaacttgtt
     2161 ggacgcgcgg gcgtggagtc cgagtttgtt gatgatttcg atggattgtt tgagcatttc
     2221 gattcccgtg ttatgtatat cggcagcaga cgccgtttaa aatgttttcc tgccctgccg
     2281 cttcttcaga cggcatcgcc gctgcgccgg cacaccaaat cttcgggcgc ggacgtgatg
     2341 gcgaaaatgc cttttaccgc cgcctccctg acgcattcgg taaaggcggc aaggtcttcc
     2401 gccgccggcg aatattggac ggccttaacc atcatcggcg cgttcagccc ggtcaaaatc
     2461 gccgatttgt tttcgcgcac gaggcggcgg gcggcattgc agggggtcgc accgaaaata
     2521 tcggtcataa tcagcacgcc gtcgttgtcg ggaaattcct gaagcgcggc aatggcgttg
     2581 ttgttgatgt cgtcttggtc ttccgtcggc tgcacgccga gtatgcggac gttttcaggc
     2641 agtccgcccg gaaaaaaatg atgcgccagc ttgcggtagg cttcgcctat ggtttcgtgt
     2701 gtgatgatta aaagccctat catattatgc gtcctgttcc tcattatcct gccggcgtat
     2761 gggcgcgatg ccgtctgaac agccttcaga cggcatcgcg ccccttattt tccgcccaat
     2821 gcgtaaatct cgcccagatt gcgccagcag cccgccgcat ccatgccgta accgaaaaca
     2881 taacggttcg gcacatccag tccgacataa tcggctcgga taggcttggg tttgtcaatc
     2941 agcttgttgg cgaacaccgc cgcacggcag cttgccgcac ccatttccaa aagtttggct
     3001 tgaatggcgg acatcgtatg cccttcgtcc aaaatatcgt ccagcacgac gacgtgcctg
     3061 ccccggattt gttccgcatc gggcatacgc ttccagttga acgcgccgcc ctccagcttg
     3121 tcgccgtaac gggaaacgtg aacataatca aaatctaagg gaaaacgcaa cagcggcagc
     3181 aactgccccg taaacaccac cgcgccgccc atcacgggca gcagcagcgg atatttgccg
     3241 cccaaatcac gcgtaatctc gtccgccact ttttgcagtg cggcacggca ttggccttgg
     3301 tcgaacaaaa gatcggcgtt ttcaagcatc gcctgtgttt caaggcgttt ggtttctaaa
     3361 tcggtcatat gtcggaatcg gtcggtaaaa ggaaaattat aaaccaaagt atcggatgcc
     3421 gtctgaactg tcctgctcag cggtcggtac gcacgcgcac aaatgtggca aatttcggcg
     3481 tgcctttccg cgtaaagcca cggtaacggt aggtaatcag tgtgccgatt ttgggcgggt
     3541 tgtcgcggtc tttatctttg aaaccgctgc cgatgcggaa ttcgccgtgc cggtttttgc
     3601 agccgaccgc gcccagccgt ccggcgtttc gccctttgcc ctcatagtgc cgcgttaccg
     3661 tgcattcgtc gtcgtattgg cttttcagct tcaataattg gctgctcctg ccgccgctgt
     3721 aacgggattc gggctgacgc agcatcacgc cttcgccgcc ctgcgcttcg atttgtttta
     3781 aaaagtccat cgcgtgctgc cggtcgcgca ctttgatttg cgggatgatg gtaatcggcg
     3841 cgttcggatg cgttttcagc cactgcgttg cgactgccaa acgttggtag aggttgccct
     3901 gcgccttggg tacatcgaaa acgtgcaggc ggatgccgcg ccagtctgaa gaaacagaac
     3961 gcacggtagc ggaaatctgc tcgaactgac cacgtccgct atacaattcg ccgtccaaag
     4021 gataaggcgg aaactgagcg gtaaaacctt tgggcggagc aaacgcgtag ccctgacggc
     4081 tcatcaggtg ctttccgtcc caataggcgc gcacgccgtc gagtttctcg ctcatcgccc
     4141 agccggcaat atcctgccct ttgtattcct gcgccagcat caaatccgcc gcgcctgctg
     4201 atgcagggat gaaaaccgcc gtaaaaatcg gtatgatgcc gccgattgtc ttcttaatca
     4261 tctgattccc ccaatatcaa aacgggcggc aaaccgccat aaaacaaacg gcaaacccga
     4321 tgccgtctga aaaaccgttt aggaacacgc cgatgaccct acgttacgaa atcttccccg
     4381 ttaccccctt ccgccaaaac tgcaccctga tttgggacga cgaaagcggc gaagccgtcc
     4441 tgaccgatgt cggcggcgac gtgccgttcc tgctgcaagc gttggcaaac cgcaaactta
     4501 cgctcacggc aatctggctg acgcacggcc atctcgatca cgcgggcggc gtggtcgaaa
     4561 tgttgaaaac gcataaagtc cctgtcctcg ggccgcatcc ggacgatgaa ttcctgctcc
     4621 aatcgctgcc gcaaaccacc gcgcaatacg gatttcccgt ctcgcccgcc tttgcgccga
     4681 accgttggct cgaagaaggc gaaacgctca cggtcggacg ctatgccttt caagtgctgc
     4741 atattccggg ccatacgccg ggacatatcg tcttttattg tgccgaggcg gaattgctga
     4801 ttgcgggcga cgtgctgttt tacgaaacca taggcagaac cgattttccg cgcggcaacc
     4861 acgccgactt aatcaataat atccgcaaca aattattcac ccttcccgaa accgtgcaag
     4921 ttgtcgccgg acacgggcgt atgacttcca tcggacacga aaagcggcac aatccgtttt
     4981 tctaaccgcc ttccctacgg tcttcagacg gcatcatctg cactgatgcc gtctgaaaca
     5041 caaaaggctc agacaaccgc cgccttgccg gtgtacctcg ccggacaagg ctttggtaag
     5101 tacttcaaac aaacccaaaa tcagaaccag tacagttacg ccgcgctttc ggcattcccg
     5161 ccccggctga aacaatattt ttccgcacaa gtcagactgc ttcatcttct gccgcgtatt
     5221 ccaaagattc cgacaacgcc gttgtttcat tggaacgctc gaccaaatcc cgttgaagtt
     5281 gtttgcctgc cttcacgccc aacagacgca gtttctcggc gcgtccgacc agattcccgc
     5341 gcccttcggc aagttgcttg aatgccgtct gaaaactgct ttgcgcctga tcgatgcctt
     5401 tgccgacgct ttcgagcgtc tgtacgaagc cgacaaactt gtcgtacagc ttgccgcctt
     5461 cgtccgcaat cgccagtgcg ttctgatttt gctgttcgtt gcgccaaata ttcgccaccg
     5521 tcctcaaagt cgccagcagc gtactggggc cgaccagcat aatccgtttg tcgaaacact
     5581 cttggaacaa gcccgcgtca ttctgcaacg ccaacaggta ggccggttcg acagggataa
     5641 acataaagac gaaatccaat gtgttcacac cttccaaatc ggtgtaatcc ttcagcgaca
     5701 agcctttcat gtgtgcacgg atgctggcaa cgtgtgccgc cagttcgcgt gccgccgtat
     5761 ccgcatccgc cgcctgcgtg tagcgcacat aagctgtcag cgagaccttg gaatcaatca
     5821 caatctgctt gttgtcgggc aggttgacca aaacgtcggg ctggaggcgg cgcgtgccgc
     5881 cgtcttcctc ttttcggacg gatgccgcct gaaccacata ttcccgccct ttctgaaggc
     5941 cggaattttc caaaaccgtt tccagaatca tctcgcccca attgccctga accttattct
     6001 gcgtaccggt cagcgcgttg gtcagggcct ttgcctcgct gtgcagctgc gcgttcaacc
     6061 cctgaagccg tttcaattcg ttttccaacg tcagccgctc gcgcgattct ttatcatagg
     6121 tttgcttgac caactcgccg aaaccgtgga tgcgttcgtt tagcgggttc aaaacctgat
     6181 ggagctgctc gcggttctgc tcggtaaaac ggcggctttt ttcttccaaa atcgtgttgg
     6241 caagattttg aaactgatcg ctcaaacttt tgcgcgcctc gcccagcaag gacagcttct
     6301 cttcagaagc aaggcgttcc tgttcgattt gcgttgccaa acgttcgttt tcaaccgcca
     6361 aaccctgtgc cttttcctgc aactcggtat gcgactgcct caaccgctcc gcttccgcct
     6421 ctttttcctg caaatgggca atctgttttt cggctgcggc aaaacggttg ccgacatcgg
     6481 aaaggtcgtt ttgcacgtcg cggacagttt ggcggctttc ttccaaatcg gtttcgattc
     6541 tttggcggat ttggcgttcc aaggcatatt ggtcggcaaa cgccttccgt tcctgtccga
     6601 gctgcgttgc caaacgttcg ttttcaaccg ccaaaccctg tgccttttcc tgcaactcga
     6661 tatacgactg cttcagccgc gccgactccg cctctttttc ctgcaaatgg gcaatctgct
     6721 tttcggctgc ggaaaaacgg ttgcccaaag cataattttc gtcctgcaaa tgccggtatt
     6781 tcccgtccaa caccgccaat tccgacacgg ttttgccgtg tgcctgttcg acaaaatcac
     6841 atcttgccgc cttttccgcc aggtgcgcgt tcaaaccggc aaactcgccc tgaaaccggc
     6901 ccttcatcag caaccatgta aacaacacgc ccgacaccaa cgccgccaaa ggcagcaaaa
     6961 cagtcatcag ttccatcaat tatcctaata ttcaaacatt ttcacaccgg acaaaaccgc
     7021 cgcttattcc gattctacct gtttgttcga catagctcca aaaaatatag cggattggct
     7081 ttaaacctgt tcgacatcgc cttaccatgc tgcttgcggt ttcagacctt ttcctaattc
     7141 aatatcaatc tgccacaaac cctgattaag ttcccgatgt ctgacatttt tagaatgatg
     7201 ccgtctgaaa tgttgcagct atgttcagac ggcatacgga ttcaggcttt tcaaacggca
     7261 ggcaaaatga aaaaagggca aaccctaaag gatttgccct tttgttccaa acgcttagtg
     7321 tacgtctttc caatattctt tatttaggaa gtaagccaaa ggcagcataa ccgcaaatag
     7381 gaaaatcatc acgacatagc ctatacgttt gcgttgcagt tgtgcaggtt cgcccatgta
     7441 cacaaggtaa ttgaccaaat cgcgtacata tgcgtcgtac tctttttgga tcactttgcc
     7501 gttaggcagg cggcggctgt gcaaaccggt agattcccaa tacagcttag gcttcatctc
     7561 gccgtgttcg tcttttacca taaccggctg acctttggca tccaactcaa cggcttgaac
     7621 accttgttgc tcccacaacg ggtggggcat accgacttta tcgaatacag tattgttcca
     7681 gccgctcgga cgggtcggat ctttatagaa gccgcgcata taggcgtaaa ggtagtctgc
     7741 acctttggaa cgcgcaatca acgtcaaatc gggcggagca gcaccaaacc attttgccgc
     7801 atctttcggg ttcatcgccg aatgcatgac atcgccgaca ttatcggtgg taaacatcag
     7861 gtttttcttg atttcttcgt cagtcaaacc gatgtctttc agacggttga agcgcatacc
     7921 gcttgcagag tggcaagaca aacagtagtt tgtaaagatt tgcgcaccgt gctgcaggct
     7981 gacttggtca cgcaggtcga tatcgacttt ttcgtagtgt ccgccgccgc tggcgacggc
     8041 tgcactcata ggcactgcca gcaataaggc agcaaaccag tttttcagag tttgtttcat
     8101 tttcgctgcc ctcatcagat attggttgca aacaagtaag caccaacaac ggtaataccg
     8161 acgtaaacaa agaacataat tttttgttta gtagtgctca tggttacgcg ttcaggaact
     8221 ggtttgttgg tatccagttt ggtatagaac ggcataccca ggaagaatgc aaagtagacg
     8281 aaagacagga tacgtgcaac caaagtacgc gtatcagttg ctaccattgc acccaaaata
     8341 cccaaaccga tgaaggcaat gatgaacaga accaatgcgg ttttgaagat tgggccgcga
     8401 tagcggacag atttaacctc gcctttatcc aaccaaggca gcaaggcgat cagtacaact
     8461 gctgcaccca taccgattac accccatacc tgagtaccgg caaaggaagg aatcgcacgc
     8521 agaattgcgt agaacggagt gaagtaccat accggcgcaa tgtgcggagg tgttttcagc
     8581 gcattcgctg catcgaagtt tggcgcttcc aagaagtagc cgccgccttc aggtgcaaag
     8641 aacatcacgg cacagaagac aatcaagaat atcgttactg ccaatatatc atgcacaaca
     8701 taatacggaa aaaaaggtat gccatctaga gggacaccgt tttcatcttt cagctttttg
     8761 atttctacac cgtcagggtt gttggaaccc acttcatgca aggcaatgat atgagccaca
     8821 accaagccga gcaataccaa aggtacagcg ataacgtgca gggcgaagaa tcggttcaaa
     8881 gtaacatcgg aaacgttgaa gtcaccgcgg atccaagtgg acaaatcagg accgataaca
     8941 gggatggcgg agaacaggtt aataattacc tgcgcacccc agaaggacat ttgaccccaa
     9001 ggcagcaggt agcccataaa ggcttctgcc atcaatgcca agaaaatcag ggaaccgaaa
     9061 atccacacca attcgcgcgg ttttttgtac gaaccgtaaa tcagaccacg gaacatgtgc
     9121 agataaacga cgatgaagaa gaaagatgcg ccggtagagt gcatatagcg gataatccag
     9181 ccgccggaca cgtcgcgcat gatgtactct actgcggtaa aggcagcagg cagatggtag
     9241 gcgttaaggt tgccgtccgg tttgtagttc atggtcagga aaataccgct gacgatttga
     9301 atcaccagca ccagcataga caatgagccg aagaaatacc agaagttgaa gtttttaggc
     9361 gcatagtatt cagacagatg ctctttccac attttactta atggaaaacg ggcatctacc
     9421 cagcctaaca atgcttttgc tttgctattg gtttggtttg ccataattat cgttccttat
     9481 tcttagtctt cgcccaccaa gatagttgtg tcgctcaagt atttatatgg cgggacaacc
     9541 aggttggtcg gggcaggaac acctttatat acgcggccgg ccaagtcgaa tttcgaaccg
     9601 tggcaggggc agaagaagcc gcctttccag tctgcaccca aatcggcggg ggcaatgtcg
     9661 ggacggaagg tgggcgagca gcccaaatgg gtgcagatac cgatggcgac aaggatgttc
     9721 ggcttaatcg aacgggtctc gtttttagca tactccggct gctgttccgc atcggaattg
     9781 ggatcggtaa gttcgccgtt caggcctttc aggtctttaa gctgctgatc tgtacggttg
     9841 agcacccaaa tcggtttgcc ttgccactcg gcggtcagca gctgacccgc ttcgatttta
     9901 ctgacatcca cctcgacggc agcaccggcg gccttggctt tttccgaagg gaaaaaactg
     9961 gccacaaacg gcgttgccac acccaatgct gccactccgc ccgcgccgca ggtcgcgagt
    10021 gtcaggaaac ggcggcggcc gttgttgatt tcttgattat ccattattca gtcgtcctaa
    10081 tattttggga ataccgagcc attaaacgtt gcaattttac ccagtttgca gtgatactca
    10141 aagcattatt taaaataagg taaagtttta tgatatttct caagactcaa gccggattgt
    10201 tttcgtcaaa atggcacact tccaacccga aaacctctgc cgccgattct gccagcgcgc
    10261 gtacgccgta acgttccgtc gcgtgatgcc ctgccgaaat gaaagccgta cccgtttcat
    10321 tggcaaggtg gtattgggct tcagagattt cccccgtcaa atacagatcg acaccttcgt
    10381 ctattgccgt ctgaaaaaac ccctgcgccc cgccgctgca ccatgcaacc cgtcggattt
    10441 cgcgttcggg attgccgata acgacaggct tacgttgcaa aactgtttca atatgcgccg
    10501 ccaatgcgcc gagtgtcttg gcttgtttca ggctgcccga gttgagcagg ttttgttcgc
    10561 cgaaccgttt ttctgtcgca aaacccaatc tgtcggcgag ttgggcattg ttgcccagtg
    10621 tgggatgtgc atccaggggc agatggtagc ctgccatatt gatgtcgtgc cgtaacagtg
    10681 cggcaatccg ttcttttttc caaccagtaa cggtcggcaa ctcgtttttc cagaacatac
    10741 cgtgatgtac caaaagcaaa tctgccttct gctccacagc aaaatcaatc gctgccctgc
    10801 ttgccgttac cgacgtaacg attttcccga tatattccct cccttcaacc tgcaaaccgt
    10861 taggggcgta atctttaaac aacgctgtct gcaatgtttc attacaccaa gtcagaaaat
    10921 ccctgcacaa taccatcttt tttcctaatc gctttaaaca agcgggcatt ctaatcgcaa
    10981 aatgtccgga attcacattt ttccgatttg cacccgcata tgaattattt taatatgcgc
    11041 cggttcaata tgccgtctga agccccatgg attccattat cgaattgcgc cacctcaaaa
    11101 ccctgctggc acttgaagaa accggcagcg tctcccttgc cgccaaacgg gttttcctta
    11161 cccaatccgc cctttcccac cagatccgta tgctcgaaaa ccactacggc acgccgctgt
    11221 tcgaacgcaa atccacgccc ttgcgcttta ccccggtggg cgaaaggctg ctgcgcctcg
    11281 cccacgaact tatacctcaa gttgctgttg cagaacgaga tttggcgcga atcacggaag
    11341 gagaggcggg agagctgcgg attgccgtcg aatgccatac ctgtttcgac tggctgatgc
    11401 ccgccatggg cgaattccgc ccgatgtggc cccaagtcga attggatatc gtatcgggat
    11461 tccaagcgga tcccgtcgga ctgctgctgc aacaccgtgc cgaccttgcc attgtttccg
    11521 aagcggaaaa acaaaacggt atttctttcc aaccgctgtt tgcctacgaa atggtcggca
    11581 tttgcgcacc agaccatccg cttgccgcca aaaacgtttg gacggcggaa gactttatcg
    11641 gggaaaccct gattacttat cccgttcccg acgagatgct ggatttgccc aaaaaaatcc
    11701 tgattccgaa aaacatcaac ccgccgcgcc gacacagcga gctgaccatc gccattatcc
    11761 aactggttgc cagcagacgt ggcattgccg cccttcccta ttggacagtc atgccctacc
    11821 ttgaaaaagg ctatgtcgtc caccgccaaa ttactgccga cggactgcaa agcaaactgt
    11881 atgccgccat ccgtaccgaa gatacggaca agagctatct gaacaatttt tgccaaatca
    11941 tacgcgaacg cggttttgca gatttgcccg gactgagcga actggaaccg gtctgacccc
    12001 ttattcaacc atacccggca gtttttctat tttttcatgt atagtggatt aacaaaaacc
    12061 agtacggcgt tgcctcgcct tgccatacta tttgtactgt ctgcggcttc gtcgccttgt
    12121 cctgattttt gttaatccac tatactgttt ttgatttttg cccaatctgt aatctttaga
    12181 ttgccaatgg gaaaccgtct actacaaata aaaaaccctg cgataagcag ggttttttga
    12241 atttccaaca ttaacgtttg gagaattgtt ttgcacggcg tgctttgcgc agacccggtt
    12301 ttttacgttc gacttcgcgg gcatcgcggg taacaaaacc agcttgagac aaggcgggtt
    12361 tcaacgcggc atcgaagtcg atcagggcac gggtaatgcc gtggcggatt gcgccggact
    12421 ggccggtttc gccgccgcca acaacattga ctttgatgtc gaaagattcg gcgttttcag
    12481 tcagaaccaa gggttggcga acaaccattc ggctggtttc ccgtgcgaag aattcgtcaa
    12541 cgggacgacc gtttacgatg atttgacctg tacctttaat caggaataca cgagccactg
    12601 aacttttgcg gcggcctgtg ccgtagtagt atttaccgtt catgtcgcgt ccttatttca
    12661 gttccaaaac tttgggttgt tgcgcagcat gggcgtgttc cgcacccgca tacactttca
    12721 gttttttaat catggcgtaa cccagaggac ctttgggcag catacctttt acagcttgtt
    12781 ccaaagcgcg gcccgggaat tgctcttgca tttcgcggaa ggtgcgttcg tagataccgc
    12841 ctgggaaacc ggaatggcgg aagtattttt tatcttcgaa tttggcaccg gttacacgca
    12901 gtttgtccgc attgataaca atgatgtaat cgccggtatc gacgtggggg gtgtattcag
    12961 gtttgtgttt gccacgcaga cggctggcga cttcggccgc aacgcgaccc aagactttgt
    13021 cttgggcatc gatgacgaac cattcgcgct tcacctcgtg gggtttcgct gaaaaggttt
    13081 tcatagtgga aatccagata gatatagaaa gttgtaaatt ttaaagacag gattcgattt
    13141 tgtcaatcgc attaccgcgt tacggaagga ttttccggat ttcggcagac tgcatactgc
    13201 tttttcgggc ggggcggcgg ccaatgtgaa aaaccgcatc gttgcgatgc ggttttgaat
    13261 gggaatcccc gcgagagccg tttcggccga atccgcttga accttgctga caaggcggct
    13321 gcctcgggta gtttcgggtg cgtccgcaaa aggacgctcg cgcccactac tgctcccggc
    13381 aaccttaagc gaacttattg gttcaaagga atatatgcct tcgcggacac cgcagggaaa
    13441 aaggggttat tcctgcgcca agcgggatag tgctttttgg caggcgttgt ccatatcggc
    13501 tattttacgc gcaaaatcgc cgattgccaa atcgccgccg ttcagggagg ttttcaacag
    13561 gtcgtggacg acgttgagcg cggccataat gacgattttt tcgctgtccg cgacgcgtcc
    13621 gccttcgcgg atggcttcgg ctttgccgtt gagcattccg actgcctgca acagtgtgtc
    13681 tttttcttct gccggcgtgt tgacggtcag ccgggcgtgc atgacttcga tgtggacttg
    13741 ttcgatgttc atcctttaat ccttattgct gcgtttcctg ccattggggg aggcgcgctg
    13801 ccagtgcgct gattttttcc ctgctctgtt cgagcaggct gcggtatcgt gtattttctt
    13861 ctgtcaggct gtcaattttg ttttgcaggt cttctttgag tttgccgact tggacgagca
    13921 gggcttcgct gagttcgtcg acggcggttt cgtgttcgag tttttgccgc tcgtgcgccc
    13981 gtttgagttc ggcgacggtt tctttgaggc ggcggttttc gctgacgagg gtttcgaatt
    14041 tttgtaccaa cgtataaacg ctgctttcga gtttttcgat attttgtttc ataaccttac
    14101 ctgtccgtat gccgtctgaa ggcttcagac ggcatctgtc tgttgtttat tcaaaacgcg
    14161 cgctgcgttc catcagtctt tcgacaacct gttgcggggt catttctttg cggatgagtt
    14221 gcagcagagt ttgggtaatc ggcatgtcga tttggtactt acaggcagta ttgaagactt
    14281 cttctatcgt gctgacccct tcggaaacgt gtccgatttc gaccagcacc tgatgcagtt
    14341 ccttgccttc tgccaaaccc aagccgacgc ggcggttgcg cgaaagtgcg ccggtgcagg
    14401 tgaggatgag gtcgccgatg cctgccagcc ccatcatggt tttgggctgt gcgcccattg
    14461 cggaggcaag gcgggtgatt tcagctaatc cgcgcgtaac cagtgcggca cgggcgttaa
    14521 gcccgtactc taggccgtcg gacaatccgg tggcaatcgc cataacattt tttaccgcgc
    14581 cgccaaccgc cacgccgata acatcggtac tgccgtaaag cctcatgacg gtcgtgttga
    14641 gctgcggtac gagttcttca atccactctt ggttttcgga ggcaaggacg acggcgcagg
    14701 gcagttgttt ggcgagttcc tgtgcaaaac tcgggccgga aagtacgccg attttcttat
    14761 tgtcgggcaa tacttctttc aagacttgaa aggtcagcag cccggtatcc tgctcgaatc
    14821 ctttgcaggc ggcgaggacg gggaggtgtc ccgcgccgta ctgtttgagc agctctgcgc
    14881 tgcttctcaa tccggcaacg gaggttacga taaggacaag tccgctgtct ttgagcgcgt
    14941 ctgccaaatc cgcacacact tccaaggttt cgggaaagga aaagccgggc agtccgcgtt
    15001 tgttttcacg cgcttcctgc atttgacgga cttggtctgc gttgcgcgtc cacagggata
    15061 cgcggttgcc gtgttgggaa aaatgcaggg cgagcgccgt accccacgaa cctgcgccga
    15121 taacggtaat tttcattggt cgtctttcaa catatcactg ccgttcactt taaaacaatc
    15181 ggtgtttctc tgcaagtgcg gtcagggaaa tgccgtctga aaggcgttca gacggcattt
    15241 tgccccgatg cggcactatc agcctgtatt gcgcaaacct tgcgccacgc cgttgatggt
    15301 caggtgcacc atcagaaggg cgtgcggatt gtcgggttct ttacgcaggc gtttgagcat
    15361 ggcgacttgc aaaccgttga gcgcgttcag gtagggaatc ctcaaagcga gcgaacgggc
    15421 gaggctgcgg ttgtcgcgca aaagctcttc ggtttgcagt aggtcgagca gtgctttgcg
    15481 gctgcggcgg tattcttcct taatcatccc gaagatgatt tttgccttat cgggcgattc
    15541 gctcaagccg gcatagtttt ccgcgagggt gatgtcggtt ttcgccatca cttgttccat
    15601 attggagagc atggcttgga agaacgggtt gctttgggcg tgttcgcgca gggcggcgag
    15661 cgtttcgggt ttgtcttcgc acaaggtttc caccgcgctg ccgaaaccgt accaagccgg
    15721 cagcatgagg cggttctgca tccaggaaaa tacccacgga atcgcgcgca agtcctgaat
    15781 ccgcgccaag gttttgcggc tggcgggacg gctgcctagg ttgagggtgg cgatttcctg
    15841 aatcgggctg gtttgcagaa agtagtcgat gaagtcggga tgggtaatca gttcgcggta
    15901 gtatttgaac gatacgtccg acaatgcctg catcagtttg gcatcagggt cttttttatc
    15961 cggcaggatg ctggcttcca aagtcgcggc aaccaaggtt tccaagttgc gttgggcatt
    16021 gccggggtcg gcgtatttgg cggtaatgac ttcgccttgt tcggtgatgc ggatttgtcc
    16081 cgccacgctg cccgccggtt gggcgagaat ggcttggtaa gaagggccgc cgccgcgacc
    16141 tacgctgccg ccgcgtccgt ggaacaggcg catacggaca tcgtattttt tgaagagttc
    16201 gaccaagccc aattccgcct gatagaggca ccatgagctg gtaacgtagc cgccgtcctt
    16261 gttggagtcg gaatagccga gcatgatttc ttggatgttt ccacggcttt cgagcagtgc
    16321 atcgtaccag tcgaggcgga acatggtttc catgaccgga caggcgtttt ccaacgcttc
    16381 aatggtttca aacagcggca cgatattgat gcggctgtgc ggtttgccgt tttccaccgc
    16441 caacaggccg gtttctttca gcagcaatgc caaggcgagc aggtcgctgg gttgttcgca
    16501 gttggaaata atgctttgtg ttacggcatc ttcgccaaat tcgtctttga ttttgcgcgc
    16561 ttcgttgaaa attgccagtt cgtggcgggt atggtcgctg tatgtgataa acgggctgta
    16621 cagaggacgt tgatggctca attcgcgcaa cagggcggtt tgtttttgct cttcgttcag
    16681 gcggttgtag tcttccaagc ctgcgtgttg gaaaagctcg gcaaccacat cggcgtgttt
    16741 gcctgcgtgt tggcgcaagt cgagcggcat catgtgaaag ccgaacacgg atacggaacg
    16801 gatgaggtct gccaaacggc cttcggcaag cagacggctg ccgttgtcga taagggaacg
    16861 ttgcaatttt ttcaaatcat ccagaaactc ttgtgccgaa gcataaggct cgagaaagcc
    16921 gaatttgcag cccataccca aaccgagcgc gcgcgctttg cccatagcgc gcgccataat
    16981 gtaggcgatg gcgcggcggt agggttcttc ggcgcgggcg atttcttcgt cgggcgattt
    17041 gtcggacaac gccgttacat cgccgttgac tttgacgcgg cggatggaga gcggcagttc
    17101 gcggtagagt ttgtcgagtt cgccgcgata gaagcggaac acggcatcgg cgtggcggcg
    17161 gaaggcaaag cgcagggttt cggcagaaac aaacggattg ccgtcgcggt cgccgccgat
    17221 ccagccgccg attttgagga tgtccggaac gcggacgccg ggataggccg tctgaaagtc
    17281 gtgttccatc ttgcggtaga gcttgggcag ggcttcgaaa aagctcatcg ggaagatgga
    17341 cacgccgttg ttgatttcgt cgttgacgct gagtttgtgg cggcgcgttt cgctggtctg
    17401 ccacaagccc agcaggatag tgtcgatttc gcggcgcagc cgtgccagcg cgtcggcatt
    17461 ggtgcagcgt tcgcgttgcg gcaacagtgc gcggatgcgg cggttgaagc ttaagacggt
    17521 ttggcgttgc acttcggtcg ggtgcgcggt caaaacggcg gtaacggacg tattgtccaa
    17581 ctgccgctgc accgatttgc cgtcggcttt ccccgctttg agcctgcgga cggtttccgt
    17641 caggctgcct tccgcgccgc cgcgtccggc ttcttcgtgg atttggcggc ggcgttcgtg
    17701 gtgcacgtct tcggcgatgt tcaaaatctg ggcgaacagg ccgcaggcca aggttaaatc
    17761 gtgggtttgt tgttcgtcca attgcggcaa tactttttca atcaatgccg cgctgtcgtc
    17821 ggaagtggac aagagtttga ctgtttcgac aaccaacggc gaggcttctt cgtgcaggag
    17881 gttgaacagg gattgtttca gaaattccgc gtccgccgcc aaagccgcgt cctttggatt
    17941 gttcagaata tgcagttgca tgatttttct ctctcgtctg ccgtaaatat tgtaaatgta
    18001 ccccaaatgc cgcatccgtg ccaaaccgtt cacactttaa ccgcccgtgt cccgaaatgc
    18061 cgtctgaagt tgaacgccgc ccgacggcag cgttacaatc gcccgcaact gtttttttcc
    18121 gaacatcatc atgaccacga ccgaacacga caacgacgat gcattcctgc tgcggtacag
    18181 ccgccacatc ctcttggacg aaatcggcat cgaagggcag cagaaacttt ccgccgcgca
    18241 tattttggtc gtcggctgcg gcggtttggg tgccgccgca ctgccctacc ttgccgcttc
    18301 gggtgtcggc acgctgacca tagccgattc cgacacggtc gaactgcaca acctgcaacg
    18361 ccaagtcgca tttgacgagg gcgatgtcgg caaactcaaa accgaagcct tggcaggccg
    18421 cctgaaacgc atcaaccata ccgtcaacgt ccgcgccgtc aacgaaaaac tcgacggctg
    18481 ccgcctgacc ggtttggttc aagccgccga catcgtttta gactgttgcg acaactacgc
    18541 cacgcggcaa gccgtcaacc gtgcctgcgt gcaaacgaaa acaccgctgg tttcaggggc
    18601 ggcggtacgc tttgaagggc aacttgccgt gtaccgtccc gacttgcccg actcgccgtg
    18661 ttacgcctgc ctgtttgacg gcggatcggc ttcagacggc atctgttctc tcttcggcgt
    18721 gttctcgccg ctggtcggca tcatcggcag tacccaagcg gcggaggctc tgaaaatcct
    18781 gctggatgcg ggcgaaccgt cgcacggcag gctggcggtt taccgtgcct tggaaggggg
    18841 ctggcaatat ttcgacctgc cgcgcaaccc tgaatgcccg gtttgcggca cagcgcgata
    18901 aaccctgccg ccgtttcaga cggcatccaa acggatgcgg cggaacggtt ttaaaaattt
    18961 aaaaatttac atttctttgc aaaaaaaaaa aaaaataaac ttaccttata attgcaattg
    19021 ttttagcaat gtctgtttcg cagactcatt gagtaaaacg ttttccccgt aatgtgtttg
    19081 gccgtctgtc ccctttgggt tcggacggct tttttttggc tgtgtttgaa tacccggttg
    19141 gttttatctg tttgcagcgg gggaagccgc ttatttccgt tcgggcggaa aacggttcca
    19201 tcggataaaa ggcattttgt ccgactgatt aaa
//
LOCUS       DAWIVB010000020        18797 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWIVB010000020 DAWIVB010000000
VERSION     DAWIVB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.020"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(49..2886)
                     /locus_tag="WIX60_000491"
     CDS             complement(49..2886)
                     /locus_tag="WIX60_000491"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ5304806.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFNLEAKVLANAEVFNFKGEAQLGFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADSGEIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(2983..3792)
                     /gene="zupT"
                     /locus_tag="WIX60_000492"
     CDS             complement(2983..3792)
                     /gene="zupT"
                     /locus_tag="WIX60_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ5304807.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTTGMAVIAVSLVLFHF"
     gene            complement(3973..5229)
                     /locus_tag="WIX60_000493"
     CDS             complement(3973..5229)
                     /locus_tag="WIX60_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ5304808.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQTKEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQNISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(5308..6699)
                     /locus_tag="WIX60_000494"
     CDS             complement(5308..6699)
                     /locus_tag="WIX60_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ5304809.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPLMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFMPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(7056..7832)
                     /gene="lpxA"
                     /locus_tag="WIX60_000495"
     CDS             complement(7056..7832)
                     /gene="lpxA"
                     /locus_tag="WIX60_000495"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ5304810.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVIGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(7919..8368)
                     /gene="fabZ"
                     /locus_tag="WIX60_000496"
     CDS             complement(7919..8368)
                     /gene="fabZ"
                     /locus_tag="WIX60_000496"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ5304811.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(8402..9448)
                     /gene="lpxD"
                     /locus_tag="WIX60_000497"
     CDS             complement(8402..9448)
                     /gene="lpxD"
                     /locus_tag="WIX60_000497"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ5304812.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPTSCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFADDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(9481..9981)
                     /locus_tag="WIX60_000498"
     CDS             complement(9481..9981)
                     /locus_tag="WIX60_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ5304813.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10047..12440)
                     /gene="bamA"
                     /locus_tag="WIX60_000499"
     CDS             complement(10047..12440)
                     /gene="bamA"
                     /locus_tag="WIX60_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ5304814.1"
                     /translation="MKLKQIASALMMLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12497..13837)
                     /gene="rseP"
                     /locus_tag="WIX60_000500"
     CDS             complement(12497..13837)
                     /gene="rseP"
                     /locus_tag="WIX60_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ5304815.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(13872..15107)
                     /locus_tag="WIX60_000501"
     CDS             complement(13872..15107)
                     /locus_tag="WIX60_000501"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ5304816.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFHPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDRITPA
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLRL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15112..15909)
                     /locus_tag="WIX60_000502"
     CDS             complement(15112..15909)
                     /locus_tag="WIX60_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ5304817.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(15912..16658)
                     /locus_tag="WIX60_000503"
     CDS             complement(15912..16658)
                     /locus_tag="WIX60_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ5304818.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(16714..17271)
                     /gene="frr"
                     /locus_tag="WIX60_000504"
     CDS             complement(16714..17271)
                     /gene="frr"
                     /locus_tag="WIX60_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ5304819.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(17451..18578)
                     /locus_tag="WIX60_000505"
     CDS             complement(17451..18578)
                     /locus_tag="WIX60_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ5304820.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(18654..18729)
                     /locus_tag="WIX60_000506"
     tRNA            complement(18654..18729)
                     /locus_tag="WIX60_000506"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18694..18696),aa:Thr,seq:cgt)
BASE COUNT         3792 a         4876 c         5567 g         4562 t
ORIGIN      
        1 aggccgtctg aaaaacattt ttcagacggc cttgtttatt caatcaaatc agtctttcaa
       61 cttcgccaac tgattttgaa cttttgccat tttgtcttcc aattccgcca aatcggcttt
      121 gtctttttcc accagatgcg caggggcttt ttcggtgtag ccgggtttgg agagtttggc
      181 gttgagtttg tccaaggctt tttgcagctt ctcggcttct ttgctcaaac gggcggtttc
      241 ggcggctttg tcgatttcga ctttcaacat caggcgcgcg ccgttgcaga cggcgacggg
      301 cgcgtcttcg ctttcgggta gggcggcgac ttgctgtgct tcggtcaggc gggtcatcat
      361 cggcaggtat ttgaggtagt ccgccaagtc gtccgtgctt tcgacaaaca gcggggcttt
      421 tacgttgggc tggatgccca tttcgccgcg caggttgcgg actgcgccaa tcaaatcctg
      481 caacacggtc atttgctcga atgccgtctg aacaatctcg ccgctgtcgg cttcggggaa
      541 gcgggcgagc atgatgctgt cggcggtttt cgcgtcgcac ataggagcga cggtttgcca
      601 cagttcttcg gtgatgaacg ggataatcgg gtgcagcagg cgcagggcgg cttcgagtac
      661 gcgcaataag gtatggcgtg tggcgcgttg gcggctggcg cagccggttt gaagctgcac
      721 tttggcgagt tccaaatacc agtcgcaata gtcgttccat acgaagctgt acagggtttc
      781 cgccgccaaa tcaaagcggt aggtttcgta ggcttgcgta acctgttcga tggtctgatt
      841 cagacggcct acaatccaca tatcggggaa ggagtagccg cgcggttcgg cagcggttgc
      901 gccgtaaccg cagtcttggt tttcggtgtt catcaagacg aagttggtgg cgttccagat
      961 tttgttgcag aagttgcggt agccttcggc gcgtttgaag tcgaagttga ccgaacgccc
     1021 caagctggcg tagctcgcca tagtgaagcg caaagcgtcc gcgcccatac tcggaatgcc
     1081 ttcggggaag agttttttcg tggcttcttc cactttcggc gcggtttcgg gtttgcgcag
     1141 gccggtggtg cgttttacca gcagtttttc caagccgatg ccgtcgatca aatccacagg
     1201 gtcaatgacg ttgccttcgg atttggacat ttttttgcct tcgtggtcgc gcacgatgcc
     1261 gtggatgtac acgtctttaa acggtacttt gccggtgaag tgggtggtca tcataatcat
     1321 acgcgccacc cagaagaaga tgatttcgta gccggttact aagacattgg acggcaggaa
     1381 ggctttgagt tcgtcggttt cagacggcca gccgagtgtg gagaacggca caagcgcgga
     1441 ggagaaccat gtatccaata cgtcttcttc gcgagtcaag cctgttttgc cggcttgttt
     1501 ttcggcttct tcctgattgc gggcaacata cacattgcct tcgttgtcgt accatgcagg
     1561 gatttgatgg ccccaccaca gttggcgtga gatacaccag tcttggatgt tgttcatcca
     1621 ttggttgtaa gtgttgaccc agttttcagg gataaagcgt accgcgccgc tatcaacggc
     1681 ttttttggct ttatcggcga ggctcaagcc tttgaactcg ctgtccggct cgccgccgtt
     1741 tggggtggcg gacatggcga caaaccattg gctggtcagc ataggttcaa tcaccgaacc
     1801 tgtacggtcg cctttcggcg tcatcagcgt gtgtggtttg atttcgacca agaaaccttg
     1861 ttcctgcaaa tcggcaacca tttgtttgcg cgcggcaaag cggtctaagc ctgcgtattt
     1921 ttcaggcagg gcaaagccta gttgcgcttc gcctttgaag ttgaacactt cggcgtttgc
     1981 cagcactttg gcttccaagt tgaacacatt aatcaggcgc gtgtcgtggc gtttgccgac
     2041 ttcgtagtcg ttgaagtcgt gtgcaggcgt gattttcacg cagcctgtgc cgaagtcttt
     2101 ttcaacgtat tcgtcggcaa tcacggggat agtacggccg gtcagcggca ggattaattc
     2161 cttgccgatt aagtgggtat aacgttcgtc ttcaggattg acggcaacgg caacgtcgcc
     2221 cagcagcgtt tcaggacggg tggtcgccac gataacggct tcggcgggat tgtccgccag
     2281 cggatagcgg atgtgccaca tagagccttg ttcttccacg ctttccactt ccaaatccga
     2341 taccgccgtg ccaagcacgg gatcccagtt caccaagcgt ttgccgcggt aaatcaagcc
     2401 ttgctcatac aggcgcacga acacttcggt tacggtttcg gcgcgcacgt cgtccatcgt
     2461 gaaatactcg cgcgtccagt cggcagagca gcccacgcgg cgcatttgtt gggtaatcgt
     2521 gccgccggaa acttctttcc attcccacac tttctccaaa aatttttcgc gacccaagtc
     2581 atggcgggac acgttttgcg cagcaagctg acgctcaacc acaatctgcg tggcgatgcc
     2641 cgcgtggtct gtgccgggaa tccaggcggt gttgcagcct ttcatgcggt agtagcgggt
     2701 cagaccgtcc ataatggttt ggttgaaggc atgacccatg tgcagcgtgc cggttacgtt
     2761 gggcggcggc agttggatgg agaaagacgg tttcgtcaaa tccatatcag gttggaaata
     2821 gccctgctct tcccagtttt gataatgttt ggattcgatt tcggctggat tgtatttgtc
     2881 taacatgatg gaactttgtg aaattaaggt tatttttgat gtgcggatta taacgcaaaa
     2941 aggccgtctg aatcatttca gacggccttt ggcatacagg ttttaaaaat ggaacaatac
     3001 caggctgacg gcaatcaccg ccatacccgt tgtcaggccg taaacggttt catggccgtc
     3061 tgaatagcgt ttggcagccg gcagcagctc gtccaacgcc aaaaacacca tcacaccggc
     3121 tatcacgccg aataccgaac caaacacggc aggcgacaaa aacggctgca aaaccaaata
     3181 gcccaaagcc gcccccaacg gctcggccaa gccggatagc agacacgccc acaccgtttt
     3241 cttacggctg cgggtggcaa aataaaccgg cgcggcgatg gaaatgccct ccggaatatt
     3301 atggatggca atcgccaagg ccaaaggcat cccgactgct ggattttcca atgtggcaaa
     3361 aaacgtcgcc aagccttcgg ggaaattgtg cgcagtaatc gcaaacgccg ccatcatgcc
     3421 gactcgcgcg atatggcggc gtttgctttc ttgaaacgac gggtcttgcg cgtctaaagt
     3481 ttcatgcggg ttcggcacca gacggtcaat cagcgcaatg ccgcccatcc cggccaaaaa
     3541 tgccatggtc gccgccgcaa acgcgtggtc tttatcataa atttcagcga acgcctcgct
     3601 ggacttactg aaaatctccg tcagggaaac atataccatc gcaccgccgg caaacgccaa
     3661 accaaacgac aacacacgcg gattgggcgt tttggaaaac atcaccaagc cactgcctaa
     3721 tacggtaaac aaaccggcag ccaatgtgat ggaaaaggca acggccaaat tggacatcga
     3781 aaaatcgggc atgagaaaac ctgcgctaaa agctgggaca ggttcagact aacacttttt
     3841 aatgtatatg ataatagtta ttatttattt tattgattgg atacacggat tttgaaacaa
     3901 aaggccgtct gaaaaatgat tttcagacgg cctttaaatt tgaaatgccg ctaaacctta
     3961 gtgctttcca gcttaagcct gataacgcga caggctcaaa tcgtcgctgc ggatttcggt
     4021 gtctttgccg ctcacgatat cggcggttaa ttttgccgaa cccagcgaca tggtccagcc
     4081 taaagtaccg tggccggtat tcagaaacag gttgtcaaag cgggtgcgac cgattaacgg
     4141 cgtgctgtcg ggcgtcatcg gtctgaggcc gctccagaac gatgcttggc tcaaatcgcc
     4201 gccttccggg aacaagtcgt tgacgaccaa agccaaggtt tcgcggcgtt tttcgggcag
     4261 tttgatttcg tagcccgaca attccgccat accgccgacg cggattctgt tgtcaaagcg
     4321 cgtgatggcg actttgtagc tttcatctaa aacggtggac accggtgcgc cgtctgaatt
     4381 ggtgaccggc agggtcaagg aatagccttt gacgggataa atgggcagat tgagatccaa
     4441 ctgcgccaaa accgtcctgc tgaagcaacc gagcgcgcag acaacggcat ctgcttcaaa
     4501 ccgccctgtt tcggtttcaa cggttttgat gcgcagcccg ttgtggtcga tgcggctgat
     4561 gttttggttg aaatgaaacc gtacgccctt ttcctgacac aatttgtata ggttttcagt
     4621 gaagaggcgg cagtcgccgg tcgcatctgc aggcaggtgc aggccgccgg caattttggc
     4681 ggtaacgcgt gccagcgcag gctcaaattc tgcacattct tcgggtttca gacggcggta
     4741 cggcacgccg tagcgttcca aaacggcaat gtcttgtttt gccgcttcga cttctttggt
     4801 ttggcggaaa atctgcaacg tccctttttt gcgtccctca aaattcatgc cggtttgcgc
     4861 ttcaaaacgg cggaacattt cacggctgta ttcggaaatc ctgaccatgc gctctttatt
     4921 ggtttgatag tgcgctgccg tgcagttttg cagcatttgc cacagccatt cgatttgata
     4981 caggctgccg tcggggcgaa acagcaaagg cggatggctt ttaaacagcc atttcagcgc
     5041 tttggtcggg ataccgggtg cagcccaagg cgtggtatag ccgtaagaaa gctggcctgc
     5101 gttggcaaaa ctggtttcca tcgccacacc ctcggcgcgg tcgatgaccg ttacttcatg
     5161 tccggcctct gccagatacc acgcggaaga cacgccggca acacccgcac ctaaaacaag
     5221 cactttcatg tttctccctc cggctttttc aaaacagact taatatgccg tgccgtctga
     5281 atattcggat tcagacggcc tcggatatta atgcggcaat tcgccgtttg tgattttttg
     5341 tttgaagtcg cgcgtttcat tgacgatgac tttcgccatc aataaaagtg caatcaggtt
     5401 gggcaatgcc atcaagccgt tgaatgtgtc cgaagccagc cacaccaaat caaggctcaa
     5461 cacggtaccc agcataacgg aagaaacata acccacgcgg tacaaaccgg caaatttctc
     5521 gccgaaaaca tacaccgcgc atttttcgcc gtaatagcac cagcccaaaa tggttgagta
     5581 ggcaaagaaa atcaggccga tggtaacaat ccagccgccg atgccgggca gcattttttg
     5641 gaatgtgacg gttgtcagtg ccgcgccgct cacttcaggt ttgacaaact cgccgcccgc
     5701 gccgagcagt cccattacca acacgatgcc ggtaatcgag caaacgacga tggtatccaa
     5761 aaacgtaccg gtcatagaaa ccaaggcctg acggacggga tggtcggttt tcgcggctgc
     5821 ggcggcaata ggcgcagaac ccatacccgc ctcattggag aacacgccgc gcgccacgcc
     5881 gtagcggatg accgtaccga tagcaccgcc cgccactgcc tgcgcgctga acgcatcgga
     5941 gaaaatcagc ttgacggcag gcatcagtgc atcggaatta atcgcgataa tggaaagacc
     6001 gcccaacaca taaaacaccg ccatagcagg cacgatgaaa gaagcggctt tggcgatgcc
     6061 tttaatacca cctaaaacga caacggcagt cagaacggtc aacgtaatgc cggtataggc
     6121 aggttcgata ccgaagctgg tttgcaccgc ctgtgcaacc gagttggact gcaccgagct
     6181 gccgataccg aaggaagcga atgtgccgaa cagcgcaaac gcgacggcca tccatttcca
     6241 gtttttgccc aagccttttt cgatgtaata catcgggccg ccggacattt cgcctttgga
     6301 attgttgacg cggtatttca ccgccaacac gccttcgccg tatttggtgg ccatgccgaa
     6361 aatggcggtc atccacatcc aaaataccgc gcccgggccg ccggttacca ccgcagtcgc
     6421 cacgccggcg atgttacccg tgccgatggt ggcggacagc gcggtcatca acgccgcaaa
     6481 atgggaaata tcgccttcgt ggccttcgcc gcttttatgc ttctttggcg gcataaacgc
     6541 ctgtttcagc gcataaccca acatcgtgaa ctgcaaacct tttaataaaa cagtcagcaa
     6601 aatacccgtg ccgaccagca gcatcagcat caaaggtccc caaacccagc cgctgacggt
     6661 ttcaaaaaag gctttgggat tgtctaaaaa cacttgcatg gctttctcct ttgtctgttt
     6721 tatttttaaa acaccacttt tgtagtgtcc agtaatttca gcacagaata tccaataaga
     6781 caatatgttc ttttgaaaaa tacttttggt tttttcgccg aaaacaggac ggttcaagtt
     6841 gcggaaattg tttgcaattc tttaaaagca gcggcggagg tcacaatgaa atgtccgaat
     6901 ggggatgtgg cgggcggcag aaatcatcaa tgctgccgac tgccatactt ctgaaatcta
     6961 caaaatgatg catcgatcaa acaatatacc gctttaaaaa aaccgatgcc gtctgaaacg
     7021 ctttcggggt ttcagacggc atcaaaaggg tacggtcagc ggatgatgcc gcgcgccgat
     7081 tgtgcgaaaa agtctcggaa tacggcaagc tcggcttggg tttcggcgcg gcggagaatg
     7141 tctgccttgg cttcttcaaa cggaatgccg cgatggtaga gggttttgta cacgtctttg
     7201 acggcggaaa tctgctctgc ggtaaaaccg ttgcggcgca tgccttcgct gttgagcccc
     7261 gccggttcgg cgcggtagcc cgatgccata aagtagggcg gcacgtcttt gtgtacgcct
     7321 gcggcaaacg cggtcatggc gtagtcgccg atgcggcaga attggaaaac cagcgtgtag
     7381 ccgcccaaaa cgacgtagtc gccgatggta acgtgtccgg caagcgaggc gttgttggcg
     7441 aaaatggtgt ggttgccgat gacgcagtcg tgcgcgaggt ggcagtacgc cataatccag
     7501 ttgtcgtcgc cgatacgggt ttcgccgatg ccggttaccg tacctaaatt aaaggtggtg
     7561 aattcgcgga tggtgttgcc gttgccgata atcagcttgg tcggctcgtc gcggtatttt
     7621 ttgtcctgcg ggatttcgcc gaggctggca aattggaaaa tgcggttgtt ttcgccgatg
     7681 ctggtgtggc cgttgatgac ggcgtgcgga ccgatttcgg tattcgcgcc gatttggacg
     7741 ttggggccga taacggtgta cgcgccgact ttgacgccgg agtcgagttc ggctttgggg
     7801 tcgatgacgg cggtcgggtg gatgagggtc atgtttttcc tttcctgtcg tgttgccgcg
     7861 aagatgcgcg acggcaacag gttgtctgaa aactttcaga cgaccttttt ctgaacactc
     7921 aaaccacgcg tttggcacac atgatgatgg cttcgacggc aacttgcccg tccactttgg
     7981 caacggcgtt gaatttgccg atgccgcgcc ggctggtcag cagctcgact tcaaagacga
     8041 gttggtcgcc ggggatgact tggcgtttga aacgggcttc gtctatgccg gcgaagaaga
     8101 agaattcgtt ttctttgcgc ccgccttcgc tcaaaatcgc caacgtgccg cacgcctgcg
     8161 ccatcgcttc gatgatgagt acgccgggca tcacgggcag gtcggggaaa tggccttgga
     8221 actggggttc gtttatggtg acgtttttaa tcgcggtcag ggttttcatc ggctcgaagg
     8281 cggtgatgcg gtcgagctgg agaaacggat agcggtgggg gatgagtttt tggatgtctt
     8341 tggcttcgat ggggagttgt acgtccatgt ctgtcgtatt ccttgaataa agtcggtttg
     8401 gttatttgct gtcttgaccg gcatctgaaa gctgctgctc cagtgttttg agccgtttgt
     8461 tcatttcgct taagcggtgg atgtaaacag cgttgcgcgc ccattcttta tgggtggaca
     8521 tcgggaagat gccggcgagg tgtttgccgc tttcggtaat gctgtgggtg acggacgtgc
     8581 cgccgccgat ggtggttttg tcggcgattt cgatgtgtcc gaccgtaccg acgccgccgc
     8641 cgatgatgca gtagctgcct atggttacgc tacctgagat gccggttttg gcggcgatga
     8701 cggtgtgcga accgattttg cagttgtgtc cgatttggac ttggttgtcg attttggtgc
     8761 cgttgccgac ggtggtgtcg ctcatcgcgc cgcggtcgat gttggtgttc gagccgattt
     8821 ctacgtcgtc gcccagcgtt accgcgccgg tttgcgggat tttgaaccac gaatcgtcgg
     8881 cgaaggcgag tccgaaaccg tccgcgccga tgaccgcgcc gctgtggatt tcgacgcgtc
     8941 tgcccagtgt gcagccgtaa taaacgacgg cgttgggatg caggacgact tcgtcgccca
     9001 gtttgcaatc gtgttggacg acggcgtttg ccaagatgcg gcagccttcg ccgagcacgg
     9061 tgtttgcgcc gatgtagacg ttcgcgccga tttcgcagct ggtgggaacg gtcgcgcccg
     9121 gttcgacgac ggcggtcgga tggatgccgc cgcgcgcttt gacgacgggt gaaaacaggc
     9181 gggcgacttt ggcgaaatag agataggggt cgtcggcgac aatcaggttg cgcccttcaa
     9241 atccgtctgc cgctttggcg gaaacgatga ccgcgcccgc gctgctgtcg tggacttcgg
     9301 ctttgtattt cggattggca aggaagctga tgtgttccgc ctgcgcgtct gcgagcgggc
     9361 gcacggcggt aacggaaatg tcctcgccgc gccattcgcc gccgagccgc gcggtgattt
     9421 gggacagggt gtaggtggcc ggaatcatgg ttttcctgtt cggtatgccg tctgaaaggg
     9481 tcagcgggcg ttcatttctt taatgacgct gtcggtaacg tcgtattggg tgttgacgta
     9541 aatcacgttc tgcaaaatga catcgtaacc ttcctgtttg gcgattttga cgatgacgcg
     9601 gttggcgttt tgctggaggg aggcaaactc ttcgttgcgg cggaggttgt agtcttcttc
     9661 aaactgcgcc tgttttttgc ggaacgctgc gaccagcccg cgccattttt cttcggcttg
     9721 cgcctttttt gcgtttctga gtttgccttc ggcaagctgc ctttccaaat ccagaccttc
     9781 gcgttgcagt ttttgcaatt cgtcctgacg agcggaaaat tcgctgtcca gcgttttttg
     9841 aatcttgcgc gcctgcttgg attcgaggta gatgcgctcg gtgttgataa agccgatttt
     9901 ttggaaggtg tcggcgtgcg cgcctgcggt gcagcacaaa ccgatcagag ccgcggcaaa
     9961 cgcgcgggtc aaacgggtca tggtaaaact ccttcgaatg ttgccgcgaa atgccgtctg
    10021 aagggcttca gacggcattt gcgggattag aacgtcgtgc cgagttggaa ttggaagcgt
    10081 tggatttcgt cttccggttt tttcttcagc gggtaggcgt agctgaattt catcgggcct
    10141 aaaggcgaga gccaggtaac cgcgccgccg gcggaatagc gcaattcgtt ggtaaaggtg
    10201 gatttatggg tattgccggc gccgtaaatg ttttgaaccc tgccgccggt cgcggaactg
    10261 ctgttgtcgt cgtaggtttt gccgtcccac acgctgcctg cgtcggcaaa caggctcagg
    10321 cggacggtgc gcgcgtcttt cgcgccgggc atcgggaaga gcagctcggc ggagacgttg
    10381 gcttttttgt tgccgccgta gctgattttt tcgccgtatt cgtcatagac tttcggaccg
    10441 agcgtgccgc tttcgtatcc gcgcaccgaa cccaggccgc cgccgtagaa gttttcaaag
    10501 aaggggattt ctttggttct gccgtagccg cccgcaatgc cgacttcgcc gccgagcatc
    10561 agcgtgaagg ttttgctcag ggggaagaac caggtttggt tgtgggtggc ggagtagtat
    10621 tgcagtttgc tgccaggcag ggcgatttcg gcgttcacgc ccgtcaggta gccgcgcgtc
    10681 ggccataacg cgctgtcggt tttgttgcgc ccccagccga cggtaccttt gtacagccag
    10741 cctttgaagc tgccgtctgt gccgtcggtt ttgccgtatt tcttgataaa gtcggcatag
    10801 tgtttgggcg ctttgttgta ggtgttgacg gtcaggtgtt ctgccaccaa accgaaattc
    10861 acgcggtcgt attcggtaac aggcacgctc atgcggatgc ctgcgcctgc cgtggtggtt
    10921 ttatattgtt tgatgctggt cgatgctttg cgcgggtcga aggcttttcc gtaaacatcg
    10981 tagcccaggc tgaccccgtc tgccgtgaag tacgggtcag taaacgacag cgagccgtta
    11041 agcgtggttt tgctcctgga ggcgcgcagt gcggccgact tgcccgtacc gaacaggttg
    11101 tcttgggaaa cgcctgcgga catgaccaac ccggtatctt gaacccaacc cgcgctcaaa
    11161 tccagggaac cggtggaacg ttcggtcaga ctcatgttca aatcgacttt gtcgggcgtg
    11221 ccggcaagcg ggacagcatc aaactggaca ttgtcgaagt agcccaaaag ctcgacgcgc
    11281 tctttggaac gttgcagctt ggaggtgtcg taaggtgcgg attccatttg gcgtaattca
    11341 cggcggacga cttcgtcgcg ggttttgttg ttgccggtga tgtgtatttc gttgacgtag
    11401 attttccggc ccggttcgat gtgcaggacg aaatcgacgg ttttggtttc agcgttcggc
    11461 agcggctgta cgctgatttc gctgtatgcg tagcctgccg agcccatgcg gttctgaatc
    11521 tcacccaaaa cggcggtcat ctgctggcgt tcgtaccatt tgccgggctt catggtcagc
    11581 agtttttcca gttcggcttt ggggacttcg ttggtgtcgc cttcgatgga gactttgccc
    11641 caacggaaac gtccgccttc gtggacggtg attttgatgg tctgcttggt tttgtcttcg
    11701 ttggtttgga tgtcggtatc gaggatacgg aaatcgaagt agccgttatt ttggtagaag
    11761 tcggttactt tttccatatc ttgggcaaat ttctgctcgt tgaattggtt gcttcgtgtc
    11821 agccatgtcc aaatgccgcc ttcggtcagg gacatttgcc gcatcagttt gcggtcggaa
    11881 tagacttggt tgccttcaaa ttcgatgtcg gtgattttgg cggatttgcc ctcgtcaatc
    11941 gtgatgtcga tgtcgacgcg gttgcgggcg agtttggtta ctttgggcgt gatttggata
    12001 ttgagtttgc cgcgcccgag gtattcttct ttcaggccgg cgactgcctg attgagtgtc
    12061 gcctgattaa agtattgcga ctgcgccagc ccgaacgatt cgaggttttt cttaatggcg
    12121 tcgttttgca gcatttttgc gccggtgatg ttgagcgagc cgatggtggg gcgttcgata
    12181 acggtcagca ggagctgccc gtccgcagtt tcgacgcgta cgtcgtcaaa gaaaccggtg
    12241 gcgtacaggc ttttgatgat ggcactgccg tgtgtgtcgt tgtaggtgtc gccgactttg
    12301 acgggcaggt agttgaatac ggtactcggc tcggtacgct gcaagccttc gacgcggatg
    12361 tcttggatgg tgaagtcggc aagtgccaaa ggcgatatgc ccaacatcat cagtgcggaa
    12421 gcaatctgtt tcagtttcat tgtcagttcc ttgtggtgcg gaatgcggtt tcagacggca
    12481 ttccgaaacg taaaatctaa ccgagcagcc gggtaacgtc gttgaagaag gcgaccgcca
    12541 tcatcagcat catgagggcg agcccgaagc gcaaaccgat gttttggacg cgttcgccca
    12601 aaggtttgcc gcgtatccat tcggcagtat aaaacacgag gtgcccgccg tccaaaacag
    12661 ggacgggcag taggttcagc acgccgaggc tgatgctgac cagtgctaaa aattccaaat
    12721 aactttgcaa gccgagttcg gcggactgtc cggcaatgtc ggcaatggtc agcggcccgg
    12781 aaatatggct gacggaggcg ttgccgctga ttagtttgcc gaaaaatttg agggttgtcc
    12841 acgagtggga aacggttttt tcccagccca tgccgaatgc gcggacaaca gacggacggt
    12901 agctgcggcg gatttgcgcg tcccacgccc tgtccggctg cggacggagg ccgacgcgcc
    12961 cgatcagggt gtggtcggac tgttcgacag tatcggggcg gatgtcggcg gtatgggttt
    13021 gtccggcgcg ttcgtagttc agggtgattt ttttgccggg gctttggcgg gtcaggtttg
    13081 cccattcttg ccatgaggcg atgggtttgc cgtcggcggc agtcagcctg tcgcccggtt
    13141 tcaggcctgc tttttcggcg gggctgcctt tttccacgcc gccggcaacg gttgtgattt
    13201 taaagggcat cagtccgatg tagccttggt tttttgcgat tttaccggct tccggcgtgc
    13261 ctgcggcatc gatggtgcgg acggtttgcg cgcccgatgc cgtctgaacg ccgacggcga
    13321 ctttgccggc ttcgaggttg aggacgattt cggtttgcgc gctgccccaa tctgcaacgg
    13381 gtgtgccgtt gacggattgt attttgtcgc cgctttggaa gccggcgcgg gcggcaatgg
    13441 tgtcgggttc gactgtgccg acgtaggggc gcagttcggt tacgccgaag gaaaagctca
    13501 gtccgtacag caaaaccgcc agtgcgaggt tggtcagtgg gccggcggcg acgatggcga
    13561 tgcgcttggc ggggtgttgt ttgtcaaaag cgtagggtaa atcggcttct gatacttcgc
    13621 cttcgcgcgt atcgaccatt ttgacgtaac cgcccaacgg aatcggggcg aggcaccatt
    13681 cggtgtcgcc gcgctttcgg gtgaaaaacg gtttgccgaa gccgacggaa aagcgtacga
    13741 ctttgacgcc gcacaatctg gcaacgatgt agtgtccgaa ctcgtgcagg ctgaccaaaa
    13801 tcaggatggc gaagataaaa gctagaaggg tgtgcaaatg gttttccttt gataacggtg
    13861 ttcagatggc atcagcgcag tgtgccgata aatgctcgcg cttgtgcgcg tgtccgggca
    13921 tcttgcgcca agagcccccc tatatcgcct atgccgtctg aaaagtcttg tgcaagacag
    13981 tgggcgacgg ttttggcaat gtcggtaaac ttaatctgtc cgtccaaaaa ggcggcgacg
    14041 gcggcttcgt tggcggcgtt caatacgcag ggcgcggctc cgcctgcgtt catggcttca
    14101 taggcgagcc tcaggcaggg gaagcggtca aagtcgggct tttggaaggt cagcgcggac
    14161 aatgcgtcga aatccaggtc gccgacaccc gaatcgatgc gctcgggcaa acccaaacaa
    14221 taagcgatgg gcgttcgcat atcgggattg cccagttgcg ccagcacgga gccgtcgcgg
    14281 tagcgcacca tgctgtgtat cacggattgc ggatggatga cgacttcgag tttgtcgggc
    14341 ggacagttga acagccaatg cgcttcaatc agctccaaac ctttgttcat catggtggcg
    14401 gaatcgacgg agattttgcg tcccatacgc caattggggt gtttgaccgc ttgggcgggc
    14461 gtaatgcggt cgaacgtgtt taaatcggcg gtcagaaacg ggccgccgga agcggtcagg
    14521 ataatcgaag cgatgccgtg ttcgttcaga cggccggcgt aatcgcgcgg caaaacttgg
    14581 aaaacggcgt tgtgttcgct gtcgacgggc agcactgccg cgccgtttgc acgggcggtt
    14641 tccataaaca acgcgccgga aaccaccagc gtttctttgt ttgccagata aatggttttg
    14701 cctttttgcg ccgctgcgag cgcggaaggc agccccaccg ccccgacgat ggcgcacatg
    14761 acaccgctga cttcgtcggc agaggcaacg tcaaccaatg cctgcgcgcc gtgtaaaacc
    14821 tgagtcgccg tgccgtcgcg tttcaacagg gcttcaagcc gggcggcgtg ttccgcatcg
    14881 gcaacgacgg catattcggg gtggaacgtt tgacattgag ccgccaattt ctcgacctgc
    14941 ttatgccctg ccagcgcgaa tacgcggaat ttttcggggt ggcgggagac aacgtccagc
    15001 gtgctttcgc ctatgctgcc ggtactgcct aatatggtca ggacttgtgg tgtcataatg
    15061 gggataactt tataccggat gccgtctgaa gcgttttcag acggcataga atcaatttaa
    15121 aaccgacatc atcgctgcat agacgctgat aacggcaatc aggctgtcgg tacggtcgaa
    15181 cacgccgccg tgtccgggca gcagcttgct gctgtctttg atgcctgccg cgcgcttgag
    15241 ccagctttcc aaaaggtcgc cgcatacgct gacaacggtc agcaccaaac cgattaacac
    15301 ggtatcgaac cagcctgtat cgaatgccag ccagccggca cttcgtacgg cggtcatgta
    15361 cactgccacg caaaccgcgc cgccgattgc accttcccag cttttgccgg ggctgattgc
    15421 cggcgcgatt ttgtgtttgc cgaacgcctt gccgctgaaa tacgcgcaaa tatcggcaac
    15481 ccacaccaaa cccatcacgg cgagcagcgg cagggcatca tcgggatgcg ggcgcaggga
    15541 tacgagcgcg aaccaaaacg gcatgaccag aagccagccg acggcataaa cctgccaacc
    15601 gccgttgagc ctccatttga atctcaacca taaaggcata acggcgagcc aaaatgccaa
    15661 aacaacatac caaaccaaat taggcagcat ccagccgccc gcataggcaa ccacgccgaa
    15721 aaccaaggtt gcggcgaggt aatggttggt tttaattttg cacaaaccgc ccatacgggc
    15781 atattcccac aaggcaatca gggcaatcag tccgcaaaat gcagcccaca accattgcgg
    15841 cgcgtaaaac agcatgccca gcatcagcgg cagcagccac atggcggtta ttacccgttg
    15901 tttcagcata ttcagttcct ttgctgttcg ataggcagtt gctcggaggt gcgtccgaac
    15961 cgccgttcgc gtttttggaa cgaagcgacg gcatcgtcca aagccttgcc gtcaaaatcg
    16021 ggccacaaaa tatcggtgaa atacagttct gcatatgcca tctgccagag caggaaattg
    16081 ctgatgcgcg tttcgccgcc ggtgcggatg aacaaatccg gttccggtgc atcgcccagc
    16141 atcaagtgtt tcgccagcgt gtcttccgta atctcggata cgccttcggc aatcagtttg
    16201 tttgccgcct gcaaaatatc ccagcggccg ccgtaatcgg cggcaatgct cagggtcagg
    16261 ccggtattgt ttgccgtcaa cgcttccgcc tcttcgatgc cttgcagaat ctgccggttg
    16321 aagcgttcgc ggctgcccaa tatcttcagg cgcatattgt tttcgtgcag gcggcgtacc
    16381 tgtttttgca aagcctgtaa aaacagcccc atcaggaacg aaacttcgtc ttcggggcgg
    16441 cgccagtttt cggttgaaaa ggcaaacacg gtcagatatt gcacacccag tttggcgcaa
    16501 tgcttcacca tattttccaa tgcgtccaaa ccgcgtttgt gtcccattat gcgcgggagg
    16561 aaacgttttt tcgcccaacg gccgttgccg tccataatca cggcgatatg cttgggaatg
    16621 gcggtgtgtt ccaaaacggc ctgcgtgctg cttttcatgt ctgcctttcg cggttcggca
    16681 ttcaaatgcc gtctgaacgc cgaaccgtgc aggttaaatt gccatcaaat cttcttcttt
    16741 ggcagtcagg agtttgtcgg cttcggtaat gtatttgtcg gtcagttttt gaaccgcttc
    16801 ttcgccgcga cgtgcctcgt cttcggaaat ttctttgtct ttgaggagtt ttttgatgtg
    16861 gtcgttggca tcgcggcgca cgttgcggat agagacgcgg ccttcttccg cttcgccgcg
    16921 tacgacttta atcaggtctt tgcggcgttc ctcggtcagc atgggcatcg gcacgcggat
    16981 caggtcgccg acagctgccg ggttcagtcc caagtttgaa tcgcggatgg ctttctcgac
    17041 tttggccgcc atattgccct caaacggttt cacgccgatg gtgcgcgcgt ccagaagcgt
    17101 tacgttggca acttggctga cggggaccat gctgccccag tattcgactt ccacttggtc
    17161 gagcaggccg gtatgcgcgc ggccggtacg cactttcgcc agattttctt tcagtacttc
    17221 gaccgaacgc tgcatcttgc cttcggctgt tttttgaata tcgttgatca tattgttctt
    17281 tcggtgggat aaggtgggcg ggagaccgtc tgaacgcgtt tcaagccgtt cagacggcat
    17341 aaagaccgtt aaccgcgaat agtaccgtta ttcgggcata acgacaaggt aggcggattg
    17401 gggatgccgt ctgaagcgac aggcgtttca gacggcatcg tgtccgaccg tcagccgtgt
    17461 tcccgtgttt caagcaggct ttggcgcagg tgttggcgtt cgtgggcatc cagccatttg
    17521 cggcgggtgc gttgcagcag gatgacgagg gcggaaattt cctgacgcat attggtgctg
    17581 agccagagga agccctgcca ttggtagtgg aggtgttcgg cgagggcttc cagttcgggg
    17641 ttgatggcgg tgtcgatgcg gatgcggcgg gcgtgtctgc cgttgataag ggcgacggtt
    17701 tgttgcaggt cggtttggag cagtgtgaag tggcggtcaa gcagccggat ttcgctgccg
    17761 ttgagtttgg gagattgcag cttggcggcg gtggtcagga gcagctcggt ggtgttgacg
    17821 attttacggt gggcgtgctg catggcttcc atcatggcgg ggctgatgcg gctttcgccc
    17881 gatgtggcgg cgagatggct gcggcttttg accatgcgtg cgttgatttg gcgcattttc
    17941 gccatgttct cctcgaggcg ttcgcgggtc atgcgcctgc cgttgctgat ttcggcaatc
    18001 attttgctgc agtcggccag gttgtcggca agcatgaaac gccacatcag tgtggatttc
    18061 agcggcagca gtttggcggc ggcgatggcg atggccgcgc cgatgaggac gttcatggcg
    18121 cgcatgagtc cgctgtcgag ccattcgctg ccgttgtcgc cgatgagcat acacatcgtc
    18181 agccctgcca gcatagggac gtagccgttt ttgccgaccg ccgcccagcc ggccagtgcg
    18241 cttgccgtgc cgacggtgag gtagaagagg aggttgccgt ggaaataatg ctggttcagc
    18301 cataaaacgc ccaaacccgc gcccagcccg atgaccgtgc cgagcatacg ttccaccgcc
    18361 ttggagtaaa tcgccccttg aaactggagc atgccgagga cgacgaagac ggtcatccct
    18421 atccactcgc cgtgttggag gtggagcagc cgggcggagg cggtggcgaa caggacggcc
    18481 ccgccgagcc ggacggcgtg gatgaggcgg cggtagcggt agcgttcgta ggagttgagc
    18541 cagcggctga cgaggcggtt gcgttgcgag gtgttcatat cggttgtgcc gtctgaagcg
    18601 gaaatgtgaa aaagcacagg cttcccgagg aagggagggt ctgtgcttgg tattggtgcc
    18661 ggagaaggga atcgaacccc cgaccttcgc gttacgaatg cgctgctcta ccgactgagc
    18721 tacaccggcg ttttttcgtc atgatatata tgaacggttg tttgtgcaac ttttcgggcg
    18781 ggcggcaagg cagtgcg
//
LOCUS       DAWIVB010000021        17962 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWIVB010000021 DAWIVB010000000
VERSION     DAWIVB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.021"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            155..991
                     /gene="cysT"
                     /locus_tag="WIX60_000507"
     CDS             155..991
                     /gene="cysT"
                     /locus_tag="WIX60_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ5304821.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGASRWTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            1243..2103
                     /gene="cysW"
                     /locus_tag="WIX60_000508"
     CDS             1243..2103
                     /gene="cysW"
                     /locus_tag="WIX60_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ5304822.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIVLATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAI"
     gene            2100..3173
                     /locus_tag="WIX60_000509"
     CDS             2100..3173
                     /locus_tag="WIX60_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ5304823.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGNILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKIHAVGALTHILVKHDKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(3230..4756)
                     /gene="ilvA"
                     /locus_tag="WIX60_000510"
     CDS             complement(3230..4756)
                     /gene="ilvA"
                     /locus_tag="WIX60_000510"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ5304824.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDELACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSHGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            4904..6073
                     /locus_tag="WIX60_000511"
     CDS             4904..6073
                     /locus_tag="WIX60_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ5304825.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNRPTVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            complement(6198..6770)
                     /locus_tag="WIX60_000512"
     CDS             complement(6198..6770)
                     /locus_tag="WIX60_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ5304826.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(6837..7820)
                     /locus_tag="WIX60_000513"
     CDS             complement(6837..7820)
                     /locus_tag="WIX60_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ5304827.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(7987..8062)
                     /locus_tag="WIX60_000514"
     tRNA            complement(7987..8062)
                     /locus_tag="WIX60_000514"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8027..8029),aa:Gln,seq:ttg)
     gene            complement(8071..8146)
                     /locus_tag="WIX60_000515"
     tRNA            complement(8071..8146)
                     /locus_tag="WIX60_000515"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8111..8113),aa:Gln,seq:ttg)
     gene            complement(8159..8234)
                     /locus_tag="WIX60_000516"
     tRNA            complement(8159..8234)
                     /locus_tag="WIX60_000516"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8199..8201),aa:Gln,seq:ttg)
     gene            complement(8242..9087)
                     /gene="ispE"
                     /locus_tag="WIX60_000517"
     CDS             complement(8242..9087)
                     /gene="ispE"
                     /locus_tag="WIX60_000517"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ5304828.1"
                     /translation="MNIADGRQAFSAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            complement(9097..9678)
                     /locus_tag="WIX60_000518"
     CDS             complement(9097..9678)
                     /locus_tag="WIX60_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ5304829.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(9818..11656)
                     /locus_tag="WIX60_000519"
     CDS             complement(9818..11656)
                     /locus_tag="WIX60_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304830.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVVKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALTAAMMYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            complement(11821..12657)
                     /locus_tag="WIX60_000520"
     CDS             complement(11821..12657)
                     /locus_tag="WIX60_000520"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ5304831.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            complement(12780..13571)
                     /gene="panB"
                     /locus_tag="WIX60_000521"
     CDS             complement(12780..13571)
                     /gene="panB"
                     /locus_tag="WIX60_000521"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ5304832.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(13713..13788)
                     /locus_tag="WIX60_000522"
     tRNA            complement(13713..13788)
                     /locus_tag="WIX60_000522"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13753..13755),aa:Thr,seq:tgt)
     gene            complement(13823..13898)
                     /locus_tag="WIX60_000523"
     tRNA            complement(13823..13898)
                     /locus_tag="WIX60_000523"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13863..13865),aa:Thr,seq:tgt)
     gene            14064..14855
                     /locus_tag="WIX60_000524"
     CDS             14064..14855
                     /locus_tag="WIX60_000524"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ5304833.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            14910..15551
                     /locus_tag="WIX60_000525"
     CDS             14910..15551
                     /locus_tag="WIX60_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ5304834.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSGGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARTPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(15629..16552)
                     /locus_tag="WIX60_000526"
     CDS             complement(15629..16552)
                     /locus_tag="WIX60_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ5304835.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            16716..17585
                     /locus_tag="WIX60_000527"
     CDS             16716..17585
                     /locus_tag="WIX60_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ5304836.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            17628..17816
                     /locus_tag="WIX60_000528"
                     /pseudo
     CDS             17628..17816
                     /locus_tag="WIX60_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4391 a         5161 c         4251 g         4159 t
ORIGIN      
        1 ttaatccact atattttaaa acatcgcgcc cgcttgagaa actgccaacc gctttataac
       61 aaattcgtct ttgcaccaaa ctttccattc tttccgtttt tcggacggca tcgttaaagt
      121 agtccttcct tttccttatt ttcagcattg ttttatgtta gcgctcaaaa cgcccggcgt
      181 actgcccggc ttcaaactca gccttggtct gaccgtattg tgcctgtcgc tgcttgtggt
      241 cttgccgttt gcgatgatgg cggcgaaggc ggcggaaatc ggctggggcg gcttttggaa
      301 cacgattgcc gagccgaacg tgttggcggc ggtatggctg agcttgcgga tgtcgtttta
      361 tgcgatgctg accaatgtcg tgttcggcac gctggtggcg tgggtattgg tgcgttatga
      421 attcccgggc aagggtctgg cgaacgcgct ggtcgatttg ccgtttgcgc tgccgacggc
      481 ggttacgggt atcgcgttgg caaccctgta tgcgcccaac ggttggatag gccgtttttt
      541 cgagcctttg ggcatcaaaa tcgcgtttac acccgtcggc atttggattg cgctggtcgt
      601 cgtcagcctg ccctttatcg tccgcgccgt gcagccggta ttggaagaat tgtcgggcga
      661 atatgaggaa gcggcggcaa ctttgggcgc aagccgttgg actacgtttc gccgtgtcct
      721 cttgcctgaa atcacaccgg cactcttgac cggcgcggga atgatgtttg cgcgggcaac
      781 gggggaatac ggttcggtga tttttatcgc gggcaacatt ccgatggttt ctgaaatcct
      841 gccgctgatt attacgggca agctggaaca gttcgacgtg cagggcgcgt cggcggtggc
      901 gttgtttatg ctgctggttt cgtttgtgat tctgtttgcg ctgaacgtga tgcagtgggc
      961 gttgggcagg cgttcgggcg cgaagggttg aggtcgtctg aaatacctgt taccgtcatt
     1021 cccgcgcagg cgggaatcca ttggtgaatt tcggctgcct tatttatttt ctgttttcct
     1081 gttgccctgc ggtggattcc cgcctgcgcg ggaatgacgg tagctagacg tttttattcc
     1141 cttaatcaat aaaaggttgt ctgaaaacga atccgcccca caaaaaacgg tttttcagac
     1201 ggcatccaaa cattttaaaa ccaaccagag aacaccaccg ccatgaaacc ctattccgcc
     1261 aatcccaacc tgaccgaacc gcgccggctg cgcgtgttgc tgattgccgc cgcgctgggc
     1321 tttctgctgc tgatgctggt cgtgccgctc gtcgccgtgt tttacgaagc cttaaaaggc
     1381 ggttgggatt tgtacctgaa atccttaaac gatcccgaag cgtggtctgc catcaaattg
     1441 acgctgatta ccgcgctgat tgtcgttccc gtcaatgccg tattgggtgt ggcgatggcg
     1501 tggctgctga cccgttttga ttttcgcggc aagcagttgc tgaccaccct gctcgatttg
     1561 ccgttttccg tatcgcccgt ggtggccggt ttgatgttcg tcttattgtt cggcgcgcat
     1621 acggcattgg gtggctggct cgaagcgcaa ggcatacaga ttatcttcgc catccccggt
     1681 attgttttgg cgacgctgtt cgttaccttc ccctttgtcg cacgcgaaat catcccgctg
     1741 atgcaggcac agggcgacag cgaagaacag gcggcattga tactcggcgc aagcggctgg
     1801 cagatgtttt ggcgcgttac cctgcccaac atcaaatggg cgttactcta cggcatcatc
     1861 ctcaccaacg cccgcgcgat gggcgagttc ggcgcggtca gcgtggtatc gggacacata
     1921 cgcggcgaaa ccaacaccgt cccgcttttg gtcgaaatct tctacaacga atacaacttc
     1981 accggcgcat tcgccctctc cggcgtattg gcacttttgg cactggcgac gctggcggtg
     2041 cagaacatca ttaccaaatt acaagacaaa aaactcgccg ccgccgaaag gaatgcaata
     2101 tgagtatcac catccaaaac ttaaacaaac acttcggcaa ttttcacgcg ctgaaaaaca
     2161 tcaacctcaa cgtccccacc ggcaaactcg tttccctgct cggcccgtcc ggctgcggca
     2221 aaaccacact tttacgcatt atcgccggac tggaaaacgc cgacggcggc aatatcctgt
     2281 ttgacgggca agacgtaacc gccaaacatg tgcgcgagcg caaagtcggc ttcgtgttcc
     2341 aacactacgc ccttttccgc catatgaacg tgtttgacaa cgtcgctttc ggtttgaccg
     2401 tattgcccaa gtccgaacgc ccgtccaaag gacaaatccg cgccaaagtc gaagaattac
     2461 tcaagctcgt gcagctctct catttggcaa aatcctatcc gcaccaactc tccggcgggc
     2521 aacgccagcg catcgccctc gcccgcgcgc ttgcggtcga acccaaactc ttgcttttgg
     2581 acgaaccctt cggcgcgttg gatgccaaag tacgcaaaga attacgcacc tggctgcgcg
     2641 acatccatca caacctgggt gtaaccagca ttctggttac gcacgaccaa gaagaagccc
     2701 tcgaagtttc cgacgaaatc gtcgtgatga accacggcaa aatcgaacaa accggcagcg
     2761 ccgaagctat ttaccgcaaa cccgaaaatg ccttcgttac cgagttcctc ggcgaaaccg
     2821 acgcttttga aggacgcatc gaaaaaggct tctggcatta caacggcttc gcgtggaaat
     2881 tggacgcgca atacaaatgg caggaacaaa ccgccaccgg ctatatccgc ccgcacgaat
     2941 ggcagatcgc cgccgaacac gaaacaccga tgatttgtgc cgaaatcgaa aaaatccacg
     3001 ccgtcggcgc attgacgcat attctggtaa aacacgacaa acaggacgta catatcacgc
     3061 tggcaggcag cgatgccgcg cgttacccaa tcgccgaagg caaagaattg aagctgattc
     3121 cgaaacaggt ttatgtcttc tctcaaaacg aactgattga atattcgatt taaccatgaa
     3181 agcgcaatgc cgtctgaaag gctttcagac ggcattgtgc tttcaagcgt caggcaagaa
     3241 acagcttgta cgcggcattt tgcgtttcct cgtgatagct gtatcccaga ctttccaaga
     3301 aaccgtcaaa tgcggcggca tcgtgcggcg gcacatcgat accgaccaaa atccgcccgt
     3361 aatccgcacc gtggttgcgg taatggaaaa gcgtaatatt ccaccctccc tgcatatggt
     3421 tcaaaaagcg tgccaatgcc cccggacgct ccggaaactc aaaactgacc aaacgctcgt
     3481 tttctacttt gtccgtccgc cctccgacca tatagcggat atggattttg gcaatctcat
     3541 tgttggtcaa atcgacattg ggcaatcccg cctcatccaa ccggctgccg ataaccgcca
     3601 aatcctgcgg gcctgccgct tgaagtccga caaagatatg cgctttttca tcgtctccgt
     3661 agcggtagtt gaactcggta atattcctat ttcccaatat attgacaaac ttaaggaagc
     3721 tgccgcgttc ttcagggatg gtaacggcaa aaataccttc gttgccctcg cccaattcgc
     3781 tccgttccga aacgtggcgc aaacggtgaa aattcatatt cgcaccgctg gtaacggcaa
     3841 tcagggtttg gttttccgcg ccttctcggg cgatataggc tttcagaccc gccaacgcca
     3901 acgcgcccgc cggctcggta atgctgcgcg tgtcatcgaa aatatccttg accgcgccgc
     3961 aaaccgcatc ggtatcgact gtaatgattt catccaaaag ttctttgcag aggcggaagg
     4021 tttcgtttcc gacgactttg accgcagtgc cgtctgaaaa cagcccgaca tctttcaaat
     4081 ggacgatttc acccgcttcg accgactgct tcatacagca ggaatcgttg gtctgaacgc
     4141 cgataacttt gatttcggga cggacctgct tgataaatgc cgccacgccc gccgccaaac
     4201 cgccaccgcc tatcggtacg aatacggcgc ggattggatc gggatgctgg ctgacaattt
     4261 ccatccccac cgtcccctgt cccgcaatca catcaggatc atcaaacggc gcgatatagg
     4321 ttaacccttc tttttccgcc aactccatcg cataatcgta ggcatcgttg tatgaaacgc
     4381 cccgcaaaac cacctcgccg ccatggcttt taaccgcatc cactttgatt ttcggcgtag
     4441 tctccggcat aacgataacg gcacggcagc ccaaacgctg tgcggacaat gccacgcctt
     4501 gagcatgatt gcccgcgctt gccgcaatca cgccgcaagc gagctcatct ttcggcaact
     4561 tggacatttt gttgtacgcg ccgcgtattt tgaacgaaaa aaccggctgc aaatcttcgc
     4621 gtttcaaaag gatgttgttt ttcaaacgta cagaaaggct gcgtgccggt tccaaaggcg
     4681 tttcgaccgc cacatcatag acagatgccg tcaggatgcg gatgaggtaa tcggaataag
     4741 gaaggggcgt gttcataatt caatatggga taatcggttt attaaaatcg caaaacccaa
     4801 aaccatacgc ccaagacggc gcgaaatcaa gaaaaatccg cccgatcaga caccctaagc
     4861 gtataatcgg cagactgaaa cacgcacaca attagaatat ttcatgacag cacataaaat
     4921 cctgcccgtc ctgctttcca tcatcttagg cgtttctcac gcaacggctg catcgcccgc
     4981 gcccaacaga ccgacggtac acgccgcccc cacgttccaa acacccgaaa ccctcacagc
     5041 ggcacacatc gttatcgacc ttcaaagcaa acagatttta tccgccaaaa acatcaatac
     5101 ccctgttgaa ccggcggcac taacccaact gatgaccgca tatctggttt tcaaaaacat
     5161 gaaatcgggc aatatccaat ctgaagaaaa cttaaaaata cccgaatccg catgggcttc
     5221 agaaggaagc agaatgtttg tacgtcccgg cgatacggtc agcaccgaca aactcttaaa
     5281 aggcatgatt gcactatccg caaacgatgc cgccctaacc cttgccggcc ggctgggcaa
     5341 cggctcgatt gaaaattttg tgcaacaaat gaacaaagaa gcccgacgct tgggcatgaa
     5401 gaacactgta ttcaaaaacc cgacaggctt gagtagagaa ggacaggttt ccaccgccaa
     5461 agacctcgcc ctgctgtctg aagcattgat gcgcgacttt ccggaatatt acccgctgtt
     5521 ttccatcaaa tctttcaaat tcaaaaatat agaacaaaac aaccgcaata tccttttata
     5581 tagggacaac aatgtaaacg gtctgaaagc cggacacaca gaaagcggcg gctacaacct
     5641 tgccgtgtca tactccggca acggcaggca catccttgtc atcacattgg gttcggaatc
     5701 ggcggaaaca cgcgcatcag acaacagcaa gctgctgaac tgggcattgc aggccttcga
     5761 tacgcccaaa atatatccga aaggcaaaac cgttgcccaa atccaaattt ccggaggcag
     5821 caaaaaaacc gtccgcgcag gcttcctcaa agaagcctac atcactctgc cacataagga
     5881 agcgaaaatg gcagaacaaa ttctagaaac catacagccg attcccgccc cagtaaaaaa
     5941 agggcaaatt ttaggaaaaa tcaaaatcag acaaaacgga tacaccattg ccgaaaaaga
     6001 aatcgtcgca ctggaaaatg taaaaaaaag aagccggtgg caaaggcttt gggcgtgtct
     6061 gacagggcag taatctgccg tttcaaatat cccgtttttc caacaaataa agaaatgccg
     6121 tctgaaacac ggttcagacg gcataaaaca acagggcggt acgtattgca tacgcgccgc
     6181 cctgctgctg aaatcaatta gcgtttctta ccggtaacgg tagcaacagc cagattttcg
     6241 ttacgtttca gggaaacgct ttctacacct tcaggcagtt tgatgtctga caagtgcaga
     6301 atgtcgccgg caaccacttc agcacaatcc aaatccaaga aagcagggat gttggcaggc
     6361 aaagcaacta cttcaacaga agtgtttaac agagatacgc ggccgccttg cagtttgacc
     6421 gcttgggaat tttcagcgtt aacgatgtgc aggggaacac ggatgcgtac aagttgatcg
     6481 gctttcacag cttggaagtc gatgtgttga acttcgcggc ggaacgggtg catttggaaa
     6541 tcacggacga taacgtcttt ggtttcaccg ttcagagaca acttaatcaa cgcagtatgg
     6601 aaagattctt tttccaatgc gtagaatacg gttttgtgat ccacagcgat tgcaacaggc
     6661 tcttgacctt caccgtacag aatgccgggg atttggcctt cgcgacgcag gcggcggctc
     6721 gcaccagtgc cttgtgcttc acgaacagag gcttgaattt cataagtcat gttaaatact
     6781 ccaagttagg taaaatcgcc gtcatcggcc gcgaccagct taagacggct tcgggcttat
     6841 ggcagcaaca tgctgcctgt catcacttct tcattgaaaa gatatgagac ggattcttca
     6901 ttgctaatgc ggcggacggt ttcggccaac agaccggcaa tcgttacctg acggatacgg
     6961 tcgcagtttt tagccgcttc agacaaagga atggtatcgg ttacgaccac ctggtcgatt
     7021 tcggatgagg cgatacggct gaccgcctct ccggagaata cggcgtggct ggcataggct
     7081 agaacacgtt cagccccccg ctctttcagg gcgacggcgg ctttgcacag cgtatttgca
     7141 gtgtcaatca tatcgtccac aatcagacag gttctacctt gaatatcgcc gatgatgttc
     7201 atgacttccg ccacattggc tttcgggcgg cgtttgtcga tgattgccaa gtcggcattc
     7261 agggattttg ccacggcgcg ggcgcggacg acaccgccga tgtccgggct gacgacggtc
     7321 agattttcaa tccgctgttg tttgatgtcg ttcaacagaa tcggggtggc ataaatattg
     7381 tccaccggaa tatcgaagaa accttgaatc tggtcggcat gcaaatcgac agtcaaaaca
     7441 cggtcgatcc ctgccgaata cagcatattt gccaccagtt tggcagaaat cggaacgcgg
     7501 acggaacgcg gacggcggtc ttggcgcgca tagccgaaat acggaatggc tgtggtaata
     7561 cgacctgccg aagcacgctt cagtgcatcc gccatcgtca ggatttccat caggttgtca
     7621 ttggtcggcg cacaggtcgg ctgaaggatg aaaacatcgc gcccgcgtac gttttccaac
     7681 agttcgacgg caacttcgcc gtctgaaaac ttggatacgg aagcattgcc caaagaaatg
     7741 tccaaatgcc tgacaacacg ttgtgccaat tcgggattgg cattgcctgt aaataccatc
     7801 aaactgtcgt acgcagccat attctcacct gattttatgt ttaacttccg ctcagaaaac
     7861 acaatgcttc cctctgcttg taccaagccc gacattatac ctgcaacatc cgacacaaac
     7921 aaaggcattt caatattttt atttctatga aataaaagcg tgtaagcagg cttacacgct
     7981 tttatttggc tggggaggaa ggattcgaac cttcgcatgc tggaatcaaa atccagtgtc
     8041 ttaaccgctt gacgactccc caaaaagggc tggctgggga ggaaggattc gaaccttcgc
     8101 atgctggaat caaaatccag tgtcttaacc gcttgacgac tccccaactc gtttgacttg
     8161 gctggggagg aaggattcga accttcgcat gctggaatca aaatccagtg tcttaaccgc
     8221 ttgacgactc cccaacaatg tttatacgga caacaaagga tgttttgaaa gaccctctgc
     8281 caaatatgcc tcgtacaaat ctgaaacttg tcggtatata ttgtatgcgc tattcctatc
     8341 ttgacacgcc gtgaatacac acgcaccgga acctgtcatt aaggcaaatc catatcggga
     8401 caactcggaa taggctttcc aaacttcagg gtattcttta aatacaactg cctgcatatc
     8461 atttctaaac ggttgcagat tttggaaagt cggcattatg cttgaggcgg aatttcgtgt
     8521 caagctttcg tgtgtgaaaa tttttgcagt ggaaacgtgg acgggcggtt tgacgatgac
     8581 ataccactgt ttcggaatat ccatttcgtc cagcctgtcg cctatacccc gcgcaaacgc
     8641 atttttgccg aaaataaaaa acggtacgtc cgcccccaga gccgcgcccg aatcaatgag
     8701 ctgccgctgc gtcagaccgc actgccacca acggttcaac accagcaaaa cggttgccgc
     8761 atccgagctt ccgccgccca aacccgcccc tgtcgggatt tttttgtcca gccatatttc
     8821 cacgccggcg gggttgcgcg catatttttg cagcaacgat gcggcacggt agctcaaatc
     8881 tacttcctgc ggcatgccat caaccggatt gtgcaggatg attttgccgt cgtccctcgg
     8941 tttcaaatat acggtatcct gcaaatctat caggcagaat atgctttcga tattgtgata
     9001 accgtcttcc cgcctgccgg taatcctcaa atcgagattc agttttgcag gtgcggaaaa
     9061 cgcctgccgt ccgtccgcaa tattcatctg tccgccttat ctcgtgcgcg ccgcacagcg
     9121 ttccggggtt tcggtttcag acggcatacc gatttcggtg aaaaccagcc tgatgttcaa
     9181 atttccgtta ttcagttgca acgttcggac ttgcccccca ctgtcggcgg ttctgccgac
     9241 agtccaaccg tattgttcca atatgccgtc cggcaggatg cggtaaggcg cgcccgccac
     9301 acgcctgcca tctgcccaga tatgcagata ttggattggc agtttgaaac cgaccagctg
     9361 cctgctcaat tcttccgcac tttccgcctg atagacattt cctttgccgt ccactgccaa
     9421 tgcgccgtcc ctgtcttgac acaactgccc gagcgtactg cccaaagggg tattgatatt
     9481 gatggtttcc acgggcggtt ggtatgtcca atcgaaattt gcatacgaac ctttcccttc
     9541 cgctttcact gccaaccgcc cttctgctgc aaaactgctg atgtgttcgg acggctgcca
     9601 caggttttcg ttattttgag gtaattgcgc gcaagcggtc aaaagcagga tgaccgatgc
     9661 ggatacggtg tgtttcatca gaatttcctt aacggatgcc atcctgccgg ccgatgggtt
     9721 cggcgtgccg tgcaaaacgc gtgcgaactg cgtgccgttt gcccgaatgc cccggcttcc
     9781 cggcaaacct tatgccgtct gaaaggatgg acctgcatta tttccgaggt tttcgggaag
     9841 gttggggcaa tgcgatgccg tgacgtttga gcgtttcccg ccatattttc ttgtctcccg
     9901 taaggtgtgc cgcctgcgtc catacgtcaa ccgcctgatc gcgttcgccc aatgcccaca
     9961 acacttcgcc caaatgggcg gcaacttcgg gctcggggtc gttttcaaac gaataccgca
    10021 gatacggcag cgcgctttcc gcgtcgcctt tcaggtaata cgcccagcct atgctgtcgt
    10081 tgacagcggt atcgtccggg ttgatttggt atgccgtctg aagcagggcg aaaccttcgt
    10141 ccaaacgttt ggaatcggtc agcaggctgt agcccagatt attcataatc tgagcgttat
    10201 cgggtgcaag cctgaacgcc ctttcaagat ctgaaatcat ttttttccgc ttgccaagcc
    10261 gatcgtaaac aactgaccgc tgtaccaatg cctctgcctg taactctgta ttactgccgg
    10321 caggcggttt ttcgataatc ttgtccaacc ccctcaaagc ctcccgttta tcgggcagct
    10381 tcgacagggc gagcatctgt attttggaca aattgtctgc cgtaaaatac cgcccctgct
    10441 gttcgggaag tttccgcacc ctgccgatct gccgcaaagc cgccctgccg ccgtccaact
    10501 cgacagccgc cgcagccgcc agcacacctt tgtcgaacag gtattccggc gcggacactt
    10561 ttttcaacca ctgcctgact ttggtgtaat cccttcggtc ggcatacatc atcgccgccg
    10621 ttagcgccgc cctgctccgc tgttcctccg tccccctgcc gtatgccttt tcggcgtagc
    10681 cgtcgataac ggaagcacct tcttttcggt ttgccgccaa tatcgctgcc tgaatataca
    10741 ggtctgcatt cggattgcgt tccaacagca cgttcaaacg cgcataggca tcatccagcc
    10801 tgtgcaggga aaccagattc ataatttcca tttcctgcca gacggccgaa aggttttggg
    10861 tgtctgtctg ctcgaaaaag ccgtcgagta tttcgggata tttgcgtgca gtcagacgca
    10921 acgtcattaa agtggggggc aatatttccg tatcgagctt cgccaaacgc tgcaaagctc
    10981 cgattgcctt ttccttttca cgcccctgta cgctgaacac cacatcggca accgccgctt
    11041 cgggcagatg ttcatatttc aacgccgcac ggcgaaccgc tttcgatgct ttttgcgcca
    11101 acccgtcctg ttgcacggcg gcttgtgcca acaataaaaa caccctgcgg ttctgtcctt
    11161 cgtccgcctg agccagcact tcttccagtc cgtccagatg ctgatttcct ctttccctca
    11221 gcacgttccg cagccacccc gcccgttttt gcgccttacc cggtataggc tcaatctgcc
    11281 gccatttctg ataaatcatt tccgcctgtt caaacgcgtt cagcgacacg gccatttcca
    11341 aggcgcgttc ggcgacttcg ggggattttg tgcgttccaa catcagcata taggttgcca
    11401 gagccgttcc cgcctgcccc ttttgcaagg cggtttcccc tcccagcaac gtaaatatct
    11461 gattaacccg ctcgcccact gccgcaagcc gtgcgcgttc gtttttgatt tcttcctcgc
    11521 tgtaacgctg ctgctttctg aaaaccttta cgacttcctt cggcggcttc gcatccgccg
    11581 cgccggcggc atacgcctgc ccggcaagca gggctgccgc gagcacagat aaaatggtga
    11641 aacgggcggg taacataatc ttcctttatc cgacagccgt ccgaaccttc agacggcata
    11701 taatcaggcg taactttatc acaagcagaa tcgtttgtag atttgcgaaa ccccgttcaa
    11761 aaaaataaat gctgtctgaa acgcaaatcc gcttcagacg gcattcccga cgcttgcagt
    11821 ttaagggagt tttatttcca aattgtcaat caggcgcgtc gtccccagac gggcggcggc
    11881 caagaccacc agtttcttat ctcccgcccg cgccacttcg agcgtatcgg cgcggcggat
    11941 ttcgacataa tcgaccaccc agccgtattc tgtcagggat tggacggcac gtttttccaa
    12001 acctgcataa tccaaactgc cctgcgccaa ggattcggca acagccttta attcgcggta
    12061 caggcgcggt gcttcgtcgc gttccgccgc actcaaatac tggttgcggc tggagagtgc
    12121 caaaccgtct tccgcgcgcc ccgtatcgac aggcactatt tcaacatcaa aattcaaatc
    12181 ctcggtcaaa cctttaatca cggcaagctg ctgatagtct tttttgccga aacaggcaac
    12241 atccggcgcg acaatattga acagcttgga caccaccgtc gccaccccgc ggaaatgtcc
    12301 cggacgaaac ttgccgcaca actcattttg cagattgggc ggttcgacgt tgtaacgctg
    12361 ttccacgttc ggatagagtt ctttctcatc gggcgcgaaa acaacggcaa caccctcggc
    12421 ttccagcttg tccgcatcct gttgcaaagt acgcggatat ttgtcgaaat cctcgccctg
    12481 accgaattgc aggcggttga cgaaaatgct gaccacgaca ctgtccgcgc gttttttcgc
    12541 ctcacgcaca agcgcaagat gcccttcatg cagattgccc atggtcggca caaatgccac
    12601 ctttcccgca tttttacgcc acgcgcgcag ttctcgaatg gtatgtatga tttgcataac
    12661 ggtaatcctt atctttcggg cttcctgccc gacaaaacgc gccgattata cgcgccgcgc
    12721 acggcaaaac aaaatgccgt ctgaaacggc tttcagacgg cattggcagc aaaccttgtt
    12781 caatctgcaa aaatatgttc cgcagcaggg aaagttttgg ctttgacttc ggcaacatac
    12841 gcccgaaccg ccgcttgaac actatcatgc ccctgcataa agtttttgac gaatttcgcc
    12901 gtcttacccg ggaaaatgcc gagcatatcg tgcatcacca aaacctgccc gtcgcaatcc
    12961 gcacccgcgc cgatgccgat ggtcggacag gaaacagttt cagttacctt ttttgccagt
    13021 tccgccggta cgcactccat cagcacgacc gccgcccccg catcgtcatg cgccttggca
    13081 tcgttaagca acgcctgcgc cttgccgccg cgcccctgaa ctttatatcc gccgaaggca
    13141 aacacggatt gcggggtcag accgatgtgc gcacaaaccg gaataccgcg catttgcaaa
    13201 aattcagtcg tttccgccat ccacacgccg ccttcgagtt taaccatatg cgcgccggca
    13261 gccatcagtt cggcggcggc ggcaaacgcc tgctccttac tctgctgata tgcaccaaac
    13321 ggcaaatcgc tgacaatcat cgcatttttt gcaccgcgtg ctacacattc ggtgtgataa
    13381 cacatatcgc gcaggctgac gggcagcgtc gatttccgcc cctgaaccgc catccccaaa
    13441 gaatcgccga ccagcagcat ttccacgccg gcatcgtcca tcagcgcggc aaaactggat
    13501 tcgtaagcgg tcagcatagc gattttttcg cccgccgcct tcattttttg cagtgtgttc
    13561 acagtaatca tcagatattg ttaccccgcc cttttcagac ggccttttca gtttgaaacg
    13621 ggctattata gtgaaatgcc cgcagccctg ccaccgaccg tgccgcaaat gcaaaacagc
    13681 ccgactgttt tacacaatcg gactgtcaaa tctggtgccg gcaccaagag tcgaactcgg
    13741 gaccccctga ttacaagtca ggtgctctac caactgagct ataccggctt actgaaatgt
    13801 tttgcaacac catcagcaga aatggtgccg gcaccaagag tcgaactcgg gaccccctga
    13861 ttacaagtca ggtgctctac caactgagct ataccggcaa agaaagcgaa ttatgcaggc
    13921 aatgccgcac cttggcaagg aatttatccg aaagccccgg caaatccgaa taagtaattt
    13981 atttgaaaag aaattaattt gaaacagaac aagcatcggc attctgataa aatatccgcc
    14041 tttcaaaccg accgttcaca ccgatggcaa gacatcccta ccgccgcctg cgtcctgcaa
    14101 aatccggctt tcccgaagtc ggcatttccg aagaaggcaa tatccgttcg cttcacttgg
    14161 gcagcgacac cgttcaaagc tcgatgaacc tcgaccaccc gtccgagctg gtgctctctt
    14221 acagccgcgc gatgatgggc tggctgctgt ttaccgacgc tcttccgcaa catatcaccc
    14281 aaatcggctt gggcggcggc tcgtttgcac gctggataga tacctacctg cccgacacgc
    14341 gccaaaccgc cgtggacatc aatccgcagg tcatcgccat tgcccgcaac ctgttcgagt
    14401 tgcctttcga gggcgagaaa tttgaaatta ttgaagcaga cggtgcagaa tatatcaaag
    14461 tcttccgcca caacaccgat gtgattttgg tggacgggtt cgacggcgaa caaatcatcg
    14521 atgcgctggt tgaagaaccg ttcttccgag actgccgcaa cgcactctct tcagacggca
    14581 tattcgtaac caactggtgg agcggcgaca aacgctacca acgcttcatc gaacggttgt
    14641 tgagcgtttt tgaagggcgc gtcttggaac ttcctgccga aagccacggc aatgtcgcgg
    14701 taatggcctt ccaaagcagc cccaaagagc aaaacataga caaactcaaa aaacgtgccg
    14761 acaaactgag caacgcatac ggattggact tccaccgtat gcttgccggc ctgaaagcgt
    14821 ccaaccccaa caacggcaag catttccacc tttaagcaac tatcttcacg gctgcatcgg
    14881 aacatgcgga aaatcaagag gaaatcatta tgctgaaacc cgaaaccata cacatatccg
    14941 gtcctgccgg cattttagaa accatccata tcccgtccga acaagtaccg gcacgcggtg
    15001 ttgccgtcat caatcatccc aaccccctcc agggcggaac aaacaccaac aaagtcatcc
    15061 aaactgccgc caaagcctta agcaaactcg gcttccactg ctacctgccc aaccttcgcg
    15121 gcgtaggcgg cagcggaggc acacatgatt acggacgcgg cgaaacgcaa gactgcctcg
    15181 ccgtcatcga ttatgcccgc gcccaacacc ccgaagcccc cgaatttgcc ttatccggct
    15241 tctccttcgg cggttatgtc gccacatttg ccgcacaagc gcgcacaccc gatttattgc
    15301 tgctcatcgg cgcggcagtc tgccactata ccgaccgtcc ggaaccgtcc gccgttccca
    15361 acgttgccaa aacgctgatg atacacggcg cggaagacga agtcgtcgaa atcgggaaag
    15421 cactgaaatg ggcggaaccg caagatttgc ccgtcattac catcgccggt tccacgcatt
    15481 tctttcacgg caaactcatc gtcctgcgcg acaccatcct ccgcttcgcg cccgtatgcc
    15541 tgaacggata aaaccccttt cagacggcat cgataatgta aaacccgtat cggcggcatc
    15601 cgccgatacg ggtttgcgtt ccctctcatt ataggcaaaa tccgctgcaa aacacccttt
    15661 cccgacccga caaaggatct gcaaacgcaa tcttttttgc caatagcttc aaaggtttcc
    15721 gataatcctc gctgcccgct tcagacggca cgggatagag cgcgtcattc aacagcggca
    15781 tacccaaacc catcatatgc acgcgcaact ggtgtttctt gcccgtatgc ggcgtaaggc
    15841 ggtaaaggct gaattccccc ctgttttcaa tcagttcgac cgtcgtatgt gcgtttggtt
    15901 cgccttccgc ctcttgcgtc gtaaaaaatt tctcacccct caccaaacgc gaaaccacat
    15961 cgagcggata cggcaaatcc gtccttgtcg gcgcaagcgc ctcatacgtt ttccataccg
    16021 ttttgttttg aaacatcgtc tgataggctc cgcgcgtggc aggattgtgc gacagcagca
    16081 tcacgcctgc cgtatccttg tccaagcggt gcagcggcgt aatgtcctca acattcaaat
    16141 gctgcaattc aggccgcaaa cgcaggcgcg tgagcagggt ttcccgcaaa aacctgccgc
    16201 tggggatgac gggcagaaaa tgcggtttgt ccaccacaat caaatgctca tcaatatgca
    16261 aaatcttttc ttcaaacgga atacgcggct cgctctcacg gctggtttca cggtaataaa
    16321 acatcacctt acccggctcg aacaaagaat gttcgtccaa cgccgcacca tccgaaccga
    16381 ccacaaaacc gctgttcaac cgcctgcgcc aatcgtccgc gcccacaaaa ggaaagcgga
    16441 tgcacagaaa atgcagcagc ggcagcccgt aaaactgctt ttcatgcggc agcaccaaat
    16501 aactgggttt gacaccgttc aacagcggaa gaggattatt gcgttttttc atgcggcgga
    16561 ttgtaaccgt ttttcacaaa cttttcagac ggcatatcga aacataattg acaaaaacaa
    16621 acatattgtt ttttatgatt taaaaattta tttagacaag attatcggat attcattatt
    16681 atcagtaaat aagaattaat atcaatagga gaaatatgaa gcgcatcttt ttgcccgcct
    16741 tgcccgccat cctgccttta tccacttatg ccgacctgcc cttgacgatt gaagacataa
    16801 tgaccgacaa gggaaaatgg aaactggaaa cttcccttac ctacctgaac agcgaaaaca
    16861 accgcgccga acttgccgca ccggtttaca ttcaaaccgg cgcaacctcg tttatcccca
    16921 ttccgaccga aatccaagaa aacggcagca ataccgatat gctcgtcggc acgctcggtt
    16981 tgcgctacgg actgaccggg aataccgaca tttacggcag cggcagctat ctgtggcacg
    17041 aagaacgcaa actcgacggc aacggcaaaa cccgcaacaa acggatgtcc gacgtatccc
    17101 tcggcatcag ccacactttc cttaaagacg acaaaaaccc cgccctaatc agctttcttg
    17161 aaagcacggt ttacgaaaaa tcgcgcaaca aagcctcgtc gggaaaatcc tggctcatcg
    17221 gcgccaccac ctacaaagcc atagatccga ttgtcctttc cctcaccgcc gcctaccgca
    17281 tcaacggcag caaaaccctt tcagacggca tccgctacaa atcgggcaac tacctgctgc
    17341 tcaaccccaa catctcattt gccgccaacg acagaatcag cctgaccgga ggcatccaat
    17401 ggctgggcac acagcccgac cgcatagacg gcaaaaaaga aaccgcaaga aacacatcca
    17461 cctacgctca tttcggcaca ggtttcggtt tcaccaaaac cacggcttta aacgcatccg
    17521 cacgtttcaa cgtttcaggg caaagcagtt ccgaactgaa atttggcgta cagcatacat
    17581 tttaagcagg ttttgatttt ccaaccttgt aatccaaagg agttattatg aaaaaacaaa
    17641 tcagctcagc cgtaatgatg ctgtctatga ttgcccccgc aatggcaaac ggcttggaca
    17701 atcagacatt tgaaaaccaa gtgttccaca cgcaggcaga tgcgccgatg cagttggcgg
    17761 agctttctca aaaggagatg aaggagactg agggggagtg gattcctaat gctgttggag
    17821 gcattatggg aggagttgga gggcatttca gctatatggc tagtgctatt gctagtggct
    17881 cgtataatcg aaatgaacat tggacagcaa ttggaggtgg tgcgttaatt ggtgcgatga
    17941 accctattaa cggaggaaga gc
//
LOCUS       DAWIVB010000022        17955 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWIVB010000022 DAWIVB010000000
VERSION     DAWIVB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.022"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..409)
                     /locus_tag="WIX60_000529"
     CDS             complement(<1..409)
                     /locus_tag="WIX60_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304837.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLSNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDSNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRN"
     gene            complement(626..1549)
                     /gene="truB"
                     /locus_tag="WIX60_000530"
     CDS             complement(626..1549)
                     /gene="truB"
                     /locus_tag="WIX60_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ5304838.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(1582..1953)
                     /gene="rbfA"
                     /locus_tag="WIX60_000531"
     CDS             complement(1582..1953)
                     /gene="rbfA"
                     /locus_tag="WIX60_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ5304839.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            2144..3388
                     /gene="clpX"
                     /locus_tag="WIX60_000532"
     CDS             2144..3388
                     /gene="clpX"
                     /locus_tag="WIX60_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ5304840.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(3491..4687)
                     /locus_tag="WIX60_000533"
     CDS             complement(3491..4687)
                     /locus_tag="WIX60_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ5304841.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLCRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            5091..5591
                     /locus_tag="WIX60_000534"
     CDS             5091..5591
                     /locus_tag="WIX60_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304842.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5676..6230
                     /locus_tag="WIX60_000535"
     CDS             5676..6230
                     /locus_tag="WIX60_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304843.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            6244..6444
                     /locus_tag="WIX60_000536"
     CDS             6244..6444
                     /locus_tag="WIX60_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304844.1"
                     /translation="MPSEELSDGIFVFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(6719..8107)
                     /locus_tag="WIX60_000537"
     CDS             complement(6719..8107)
                     /locus_tag="WIX60_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ5304845.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
     gene            8435..9382
                     /locus_tag="WIX60_000538"
                     /pseudo
     CDS             8435..9382
                     /locus_tag="WIX60_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(9435..9767)
                     /gene="trxA"
                     /locus_tag="WIX60_000539"
     CDS             complement(9435..9767)
                     /gene="trxA"
                     /locus_tag="WIX60_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ5304846.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            10065..10538
                     /locus_tag="WIX60_000540"
     CDS             10065..10538
                     /locus_tag="WIX60_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ5304847.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            complement(10608..11429)
                     /gene="nadE"
                     /locus_tag="WIX60_000541"
     CDS             complement(10608..11429)
                     /gene="nadE"
                     /locus_tag="WIX60_000541"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ5304848.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGAHQTAFDSQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            11626..12981
                     /locus_tag="WIX60_000542"
     CDS             11626..12981
                     /locus_tag="WIX60_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ5304849.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAVRLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIESCTVPVVSGVGHETDFTLADFVADMRAPTPTGAAELVSPNRQESLHRLAQ
                     AQCRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNVRT
                     HTARFERQTQALKHCRPDVSVCKKNIDRFQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            13274..14815
                     /locus_tag="WIX60_000543"
     CDS             13274..14815
                     /locus_tag="WIX60_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ5304850.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(14884..15933)
                     /locus_tag="WIX60_000544"
     CDS             complement(14884..15933)
                     /locus_tag="WIX60_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ5304851.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKPQNVPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(15942..16169)
                     /locus_tag="WIX60_000545"
     CDS             complement(15942..16169)
                     /locus_tag="WIX60_000545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304852.1"
                     /translation="MQIHLLLLLSFERFYSRTQTDFNRRTNFAPTPSRSDDFIRKTAA
                     SGTISNRPTEDGILSTRPAAHAAEEPPYQAS"
     gene            16158..16790
                     /gene="pdxH"
                     /locus_tag="WIX60_000546"
     CDS             16158..16790
                     /gene="pdxH"
                     /locus_tag="WIX60_000546"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ5304853.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(16920..17930)
                     /locus_tag="WIX60_000547"
     CDS             complement(16920..17930)
                     /locus_tag="WIX60_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ5304854.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLTAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDININIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANLPDQEGILE
                     LHIRRHENGVCSKMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSNRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRNGHVQDIAAQDHPDLSEYEVFACGSPAMTEQTKNLFVQQHKLPENLFFSDAFTPS
                     AS"
BASE COUNT         3990 a         4648 c         4987 g         4330 t
ORIGIN      
        1 tattgcgttt aaaataaatt tcttcgcctt cctcggtgct ttgtccgcca tttaacacac
       61 aatctttaaa cggaaaatcc aacacaatat cactgctgtc tttcaaaaag cggttgctgt
      121 ccgtcaaacc aatgcggttg gcgtattttg tgtaggaatt attgacgctg tgtttgtcca
      181 agaaaaaacg gaaatcctgc aatttaaaca ccagcacacc attcacttcc acaaagaaat
      241 ggcgttttaa ctcatcatta ctcaataaca aaccgataat ggtcgggtcg gttttttcca
      301 ccaaatccaa caggatgttt ttggctaaaa tggttttttc ttcgttcgcc catacttttt
      361 cgtgtggtag tagggcttgg gttaattcgg tttgaatgtc tgtcttcatc gttataaatc
      421 ctcatcagtt attttaatgt gccattcttg tgtaacggca aatggctttg caaatagatt
      481 ctaataaaat caattatttg atgtctttgc tgtttccggg ggcgttcgca aagttgcgat
      541 aagcggggcg ggtgtccgag tgtgtcggga ttttgcggaa atatcaacac attattcaag
      601 cacagcctgt atttttaacc gccgttcagg cggcggatgc cgccgtgttc atcaggcgca
      661 aggctttcag acggcatatt tctttttgat attccgccag accgacaaag cggccgtttt
      721 ccgtgtaaac gcgtaccggc gtgtcggaag gcaggtcttc ttcgaaacgc ggacgttgtc
      781 cgcagtggag catatggacg gcataatcgt ttaaaacggt ttggggaaag tgtgaaacca
      841 atacgtcgca gggtagcagc aagctgtcgc gttctgtttc gttcaaattt gccaaggcct
      901 caagcgtgtg gctttgggcg atggtaaagc cggcggtttc agtgcggcgc agggcggtca
      961 ggtgggcgaa cgtgccgatg tgtttggcga tgtcttcgct gagggtgcgg atgtaggtgc
     1021 ctttgctgca acgtacgtcg atgacggctt tgggcgcgtc aaattcggca atatcgatgg
     1081 cgtaaacggt aatgtagcgc gctttgcgtt cgatgacgat gcctttgcgg gcgtattcgt
     1141 acagcggttt gccttcgtgc ttgagcgcgg aaaacattgg cggcacttgg cggatgttgc
     1201 ctgtcagtgc ttggcaggcc gtctgaaatt cggctaagga aatatcggcg cgggcggtgg
     1261 caatgatttc gccttcggca tcacccgtgc tgctggcttc gccgagtttc agcgtggcgg
     1321 tgtaggcttt gtcggcatcc agcaggtatt gggcgaactt ggtcgcttca ccgaagcaga
     1381 cgggcaaaag tccggttgcc aaagggtcga gcacgccggt atgtccggct ttttcggcat
     1441 gaaacaaacg ccgcgctttt tgcagcgcgg tgttgctgga aaggccttcg ggtttgtcca
     1501 agagcaaaac accgttgacg gcacgttttt ggggtttgcc tgtattcatg gttcgatgtt
     1561 cggacggcat gggcatatcc gtcagtcttc aaccggtttt tccgccgcta cttggtcgat
     1621 aagggcggac aggttcaaac cgcgttccaa agattcgtcg tatttgaaat gcagttcggg
     1681 cgttttgaac agcttgatgc gtttggccaa ttcgctgcgg aggtgtccgc gcgcgtgttc
     1741 cagcacttct tccgtaattt cgcgcgcgtc ttggtttaaa atggtgtaga acacggtggc
     1801 gtggctgtaa tcgcgggtaa cttcgacttc gttgacggta atgaagccgg cgcgcgggtc
     1861 tttcagtccg gtacggacga gttcggcaag ctcgcgcata atttgttctt tgacacggtc
     1921 ttggcgggca tagccgcgtt ggggttttct catgaatatc ctcagatttc agatttggtt
     1981 tcagacggca tttttgccgc cggggcggtt gcgaaagggc gtattatagc ctaatcggcg
     2041 ggattttaag gtaatacatt gataagaacg gatatagtta ttatacttgg caacccgaac
     2101 ggcacgtgcc gttttctgta acgcatacat tgtaaagaaa gccatgtcca acgaaaaccg
     2161 tacttgttcc ttttgcggaa aatccaaatc acacgtcaaa catttaattg agggcgaaaa
     2221 cgcctttatc tgtgacgaat gcgtctcaaa ctgtattgaa atattgcatg aagacggaaa
     2281 tgacggcacg ccttcggaaa gtgccggagg ggagccggaa gaatccggca agctgcccac
     2341 gcccgccgaa atcgtcgcca acctcaacga ccacgttatc ggtcaggaac aagccaagaa
     2401 agccttggcg gtgtcggtgt acaaccatta caaacgcctg cgccatccta aagcaggagc
     2461 taatgtcgaa ttgtcgaaat ccaacatcct gcttatcggc ccgaccggat cgggtaaaac
     2521 cctgctggcg caatctttgg cgcgcaaact ggatgtgccg ttcgtgatgg cggatgccac
     2581 aactttgact gaagcaggtt atgtcggcga agacgtcgag caaatcatta ccaagctttt
     2641 gggcaaatgt gatttcgatg tcgaaaaagc ccagcgcggc attgtctata tcgacgaaat
     2701 cgataaaatt tcacgcaaaa gcgacaaccc gtcgattacc cgcgacgtat ccggcgaagg
     2761 tgtgcaacaa gccttgttga aactgattga aggtacagtg gccagcgttc cgccccaagg
     2821 cggccgcaag catccgaatc aggaatttat caacgtcgat accaccaaca tcctgtttat
     2881 ctgcggcggc gcgtttgcag gcttggaaaa agtcatccgc cagcgtactg aaaaaggcgg
     2941 tatcggtttc ggtgcgtccg ttcacagcaa ggacgaaaat gccgacatta ccaagctgtt
     3001 cggcatcgtc gaaccggaag acttgattaa attcggtctg attccagaat taatcggacg
     3061 tttgcctgtg attgcaactt tagaagaatt ggacgaagac gcgctgatta atattttgac
     3121 cgagccgaaa aatgctttgg tcaagcagta tcaggcgttg ttcggtatgg aaaatgtcga
     3181 actggagttt gaagaaggcg cattgcgttc catcgcgcgt caggcaatgg aacgcaaaac
     3241 cggcgcgcgc ggcctgaggt ccatcgtcga acgctgcctg ctcgatacga tgtaccgcct
     3301 gcctgatttg aaaggcttga aaaaagtggt ggtcggtaag gcagtcatcg aagagggcag
     3361 ggaaccggaa ttggtgttcg agtcctgatg gcaaacaaaa ccgcttcgga acgttgaacc
     3421 acgtttctga agcggttttt tgtttctgat gccgtctgaa gccttcccgc cttcagacgg
     3481 catttgagtt ttacggattg tctgatgtcg cggcaaattc cgtcagcgcg tgttccaaac
     3541 gtcgcaaacc ttccgccata tcctcatcgt tcaacagtag cgaaggcgcg aaacgcaata
     3601 cgtccgcacc cgcaaccaaa atcatcacgc cgtgtttcaa ggcggcggcg gtgatttcgg
     3661 atgcgcgtcc gcgataggct tcgtccaaca cgcagccgag tagcagcccc atcccgcgaa
     3721 cttgtgagaa caagcccgtt ttcctgcaca aatccagcaa tgccgtctga agtttctgcc
     3781 cctgttcacg gacatggttt aaagtttcgg gcgtattgat gatgtcgaat gcgcggctgc
     3841 cgaccgcaca cgccatcggg ttgccgccga aagtcgagcc gtgcgtgccc ggttggaagg
     3901 cggcggcaat cttttctgtc gccagcatcg cgccgatcgg aaagccgcag cccaaggctt
     3961 ttgccgaact caaaatatcg ggaacaatgc cgtaatgttc ataggcaaac agcctgcccg
     4021 tatgccccat cccggtttga acttcgtcca aaatcaacaa cgcattgtgc cggtcgcaca
     4081 gacggcgcgc ggtttgcaaa tattccgcag tggcgggcag gatgccgctt tcgccctgta
     4141 tcggctcgat gatgaccgcg caggtctgtt cgccgacggc agcttccagc gcggcaatat
     4201 cgttgaacgg aacgtgcgta atgccttgcg gcaggggtgc ataatccttg ctgtatttcg
     4261 gctgaccgcc gacggacacg gtaaacagcg tgcgtccgtg aaaactgttg atacaggcga
     4321 cgatttcgct ttttcctccg ccgaaacggt cgcgggcgta tttcctcgcc agcttcaacg
     4381 ccgcctcatt cgcttccgag cccgagttgc agaaaaaaac cttgtcggca aaactgtttg
     4441 caaccaattt ttgcgccaat tcctgcgctg gacgcgtcgt atagatattg gaaatgtgcc
     4501 acagcttgtg catctgcgcg tttaaagcat cgacaagggc agggtggcag tgtcccagcg
     4561 cattgacggc gataccgcct gaaaaatcaa tatattcacg ccctttcgta tcccaaacgc
     4621 ggctgcctga agcgcgttcg ggaatcatcg gggcaaaggc gaaattgggg gtcagatagt
     4681 tttgcataat tgctcctgat tgcggttttg ctcgggatta tgcaccgttt ttcaaagcag
     4741 ataaagggaa attgccgaca atatcgggca gcggtaaaaa taaaccgaaa tattttcaat
     4801 cggatagatg aaaaatcaaa aaaatttcga taaaatgctt gaggcgcggc agcgttttga
     4861 tttagaatct ccccaatatt tttatcttca gacggcattt catcatgcaa ggcaaaaaat
     4921 ttacaaccgc ttattgctat tggtaccaca cagacgtgcg gtactttttt gcgcttgctt
     4981 gaaatccatc ccatacgcaa aacaggccgc tcgaaaacgg ggcggctttt ttgttacctt
     5041 cacacaggtc gcccgattcg gcaacacacg acagaatcag gaaaatgacc atgaatgcaa
     5101 tgccccaaac cgccgcacaa aacgaaaccc tgcacacttg tagcattatt gtcgatgatg
     5161 cacaggattt ttcccccatg cttctcgatg cagggcggga cgacacactt atcagcgaat
     5221 cgatgattcc gcaaatccgc accgttgccg ccctgatagc cgccgaacgg cacgacttca
     5281 gccgcagcag ccccgccgag tttaccgatg ccgccgattt ttttgccgca cggattctcg
     5341 tattgggcgt gcgacgcttc catctggacg tatcgctgct tcagattttg aaaaccgcca
     5401 acagccgcgc ccgccgcttt gccgaaaaac accgcctgtt tttcacaccc gcacaagccg
     5461 aattgagcct gaacaaacac aaaaaccgcc gcctgctgac cgtagaaacc gaacacgaag
     5521 tcgaaaacaa aggcaaccca gtcgccaaca gcctcgcatt tgtccgcaaa ttgcacaccc
     5581 tgcccctgta aacatcacga tttgccggca gaatgcttgc caaaattccc gacggcgata
     5641 caataaaacc gattttccac ccaaacagga cacacatgaa aaaaatcatc gcctccgcgc
     5701 ttatcgcaac attcgcactc gccgcctgcc aagacgacac gcaggcgcgg ctcgaacagc
     5761 agcagaaaca gattgaagcc ctgcaacagc agctcgcaca gcaggcagac gatacggttt
     5821 accaactgac tcccgaagca gtcaaagaca ccattcctgc cgaagcacag gcaaacggca
     5881 acaacgggca acccgttacc ggtaaagacg ggcagcagta tatttacgac caatcgacag
     5941 gaagctggct gctgcaaagc ctggtcggcg cggcggcagg cgcgtttatc ggcaacgcgc
     6001 tggcaaacaa attcacacgg gcaggcaacc aagacagtcc cgtcgcccgg cgcgcgcgtg
     6061 cagcctacca tcagtccgca cgccccaatg cgcgcaccag cagggatttg aacacgcgca
     6121 gcctccgtgc aaaacaacag gcggcgcagg cgcagcgtta ccgcccgaca acgcgcccgc
     6181 ccgccaatta ccgccgcccc gctatgcgcg gtttcggcag gaggcggtaa acccggcgcg
     6241 ccaatgccgt ctgaagagct ttcagacggc atttttgtat ttgttaggga cattgttatg
     6301 ttgccgtttg attttcagac ggcattttgt ttccaagcgt ttgatgtcgg gatggcaatt
     6361 ctgatttacg tgcgttggca gggtggcatt gtcatgccgc cgttgggttt tcagacggca
     6421 tcatatcgaa cagtcaagcc gtagggcgag cattcttgcc caccgttgca agggcgggat
     6481 tgggtgggca ggaatgccca ccctgcatct gaaacccttc cgtgtcattc ccgtgcagga
     6541 aggaatctag atatttgaat ttacggaaat tttcaatcgt tgccaaagcg gaaaagggtt
     6601 tttattacag cctaatcctt tgggtatcaa agccgggcgg tgctgaaaca ttgcaaaaat
     6661 gccgtctgaa actcgaaatc ggtttcagac ggcattggtt tatgcggcag gttggggctt
     6721 agcggtcttt gtaacggcgt tcgcctttgc ctgcaaaaga atcgccggct ttgcgtttgc
     6781 ctttgaagcc ttcgccggtt tttttgaaac cgtctctctt aaagccttct ttcttgaaac
     6841 cttcgccgcg cgttttgccg ccgaagcctt ctttgctcgg tttatgatcg ccgcgccaac
     6901 cgccggattt acgatcgccc cagccgcctt tgcctttcgg cttgccgcct gcggatttgc
     6961 gtttgcgggt cggttccatg ccttcgatgg tcagttcggg cagttttcgg ttaatgtatt
     7021 tttcgatttt gtggactttg acgtattcgt tcacttcggc aaacgtaatc gcaatacccg
     7081 tgcggcctgc gcggccggtg cgcccgatgc ggtggacgta gtcttccgcc tgtttcggca
     7141 ggtcgtagtt gataacgtgg gtaatggtcg gtacgtcgat accgcgtgcg gcaacatcgg
     7201 tggcaaccaa aattttgcag cggcctttac gcaaatccat cagcgtgcgg ttgcgccagc
     7261 cttgcggcat atcgccgtgc aggcagtttg cggcgaaacc tttttcgtac agttcatccg
     7321 caatgacttc ggtcatggct ttggtggacg tgaaaatcac gcattgatcg atattggcat
     7381 cgcgcaagat atgatcgagc aggcggtttt tgtggcgcat atcgtcgcag tacagcagtt
     7441 gttcttcgat tttgccttga tcgtccacgc gttcgacttc gatgatttca gggtctttgg
     7501 tcagtttgcg cgccagtttg ccgaccgcgc cgtcccaagt ggcggagaac aacaaagtct
     7561 gacggtcgct cggcgttgct tccacgatgg tttcgatgtc gtcgataaag cccatatcca
     7621 acatacggtc ggcttcgtcc aaaatcagca cttccaaacg ttcaaaatca actttgccgc
     7681 tttgcatcag gtccatcaga cggcccggcg tggcgacaat cagatcgacc ggtttgctca
     7741 gggcacgggt ttggtagccg aaagacgcgc cgccgacgat gctgacggtg cggaaccaac
     7801 gcatattttt ggcatacgcc agcgcgtttt tctcgacttg agccgccagt tcgcgggtcg
     7861 gggtcaacac caaagcacgc gggcctttgc ccggtttttc gctgcgtttg gtcagttttt
     7921 gcaaagtcgg taacagaaag gcggcggttt tgccggagcc ggtttgcgcc gaagccatga
     7981 tgtcgcggcc ttccaaagca aacggaatgg cttgcgcctg aatcggcgtc gggctttcgt
     8041 aaccctcgct gctgacggcg gacaaaatgt ttttatcaag gttcaaatcg gcaaatttaa
     8101 tagacatggc tatccttgga agacaacaac gcccgcgtcc gaaaggttca gacggcctga
     8161 agcagggaag ggtaacgata cggggaatgt gaagttacag ggttgtcgca agcggcttgc
     8221 ttgtggttta catcgacggc aacctgcaca gatatgcttt gcaaaatcaa agcaacagaa
     8281 gaaacgcgcc cgtaagggcg gtttaggttg gaaggcgatg gcttcgtatg acaagataag
     8341 cgacggatgg tatagggttt gcccgaaacg gtcaagcaat cgggcgttaa ttacagtaaa
     8401 attgccgttt tccactttat tccgcctgaa accaatgacc gacatcacac cgtttgccaa
     8461 ccgcttgggc aaaaacatca aacaccttat gaaatgggcg aaacgcaacg gcatcgaagc
     8521 ctagcgcatt tacgaccgcg acatccccca attccccttt gccgccgatg tttacggcga
     8581 tcgaatccac cttcaggaat acgataccgg ctggctgatg cggcccgaag aatacgaagc
     8641 gtggcttgcc gaagtcttgg aagccgtcgc tttcgttacc ggttttgcgc ccgaacaaat
     8701 ccgcctcaaa cgccgcgaac gccaaaaagg tttgcagcag tacgagaaaa ccggcaaggc
     8761 gggcgacgat ttcgtcatca ccgaaaacgg ccgcaagttt tgggtcaacc ttgataagta
     8821 tttggatacg gggctttttt tggatcatcg aaacacgcgc aaaaaagtcg gcgaaacggc
     8881 ggcgggcaaa cgttttttaa acctgttttc ctacaccggc agcttcaccg tctatgccgc
     8941 caccggcggc gcggcatcca gcgaaaccgt cgatttgtcc aacacatatc ttgattgggc
     9001 gaaacgcaat tttgaattga acggcatcga tacggaacga cacaaaatcg tccgcgccga
     9061 cgtgttccaa tatcttcaga cggcatatgg ggaaggcagg cggttcgacc tcattgtgat
     9121 ggacccgccc agtttttcca acagcaagaa aatgtccgac atcctcgaca tccagcgcga
     9181 ccacaaaaag ctgattgacg gcgcggtgaa gctgctcgct tcagacggca ttttgtactt
     9241 ttccaacaac ctgcgcagct tcgtcttaga cgacttggta tcggaacaat acgccgtcaa
     9301 agacatttcc aaacaatccg ttcccgaaga tttccgcaac aaaaaaatcc ataggtgctg
     9361 ggaaatccgc cacaaatctt aagggtgcga aaaagccgcc ggaatcttcc ggcggctttt
     9421 tcgattgcgc tttatcaggc gatagaggct tcgacaaagg cggtcagctg accttttgcc
     9481 aatgcgccga ctttggtggc gacgacttcg ccgtttttga acaccatcag ggtcggaatg
     9541 ccgcgcacgc cgaaacggga cggggtggct tcgttgtcgt cgatgttgat tttgaccact
     9601 ttcagacggc cttcaaattc ggcggcaatg tcgtccaaaa tcggggcaat cattttgcag
     9661 gggccgcacc acggagccca aaagtccagc aggacgggga tatctgcatt taaaacgtct
     9721 ttttcaaaag cggcatcgct ggtgtgtaca atcaattcgc tgctcattat ttttcctttt
     9781 cggttgggat tggcacgata gcgtgcagga tagggcttgg gcataaaaat ttcaagtggg
     9841 cgggcgggat aatagttttt ctgtaccgcc ccaatcgcct ttgcaaattc taaaaaacgg
     9901 ttcggacagt attttatttc tgaaaattac ttttattatc aaaatgtaag gaatacgttt
     9961 gaggctgtac agaagcgcgc gggcggtgta tgctccgttt gaattgattc ttaaagtatg
    10021 aatgttcaaa accaaacccg aaaccctgta aaaggaacac tgcgatgact gatttttccg
    10081 tttgggagac cgcccccttt ggtgcaaccg tcgatcatat cctgcaacgc taccacaatg
    10141 tccaccgtgc acaatttgaa gaattggtgc cgctggcgca aaaagtggcc caagttcatg
    10201 ccgacacctt tccggcggaa attgccgagc tgcttgccta tatgcagaac gaactgctga
    10261 tgcatatgat gaaagaggaa aggatgctgt ttccgatgat taatcagggt gtcggacgcg
    10321 gtgcggcgat gcccatcggc gtgatgatgc acgaacacga agaacacgac cgcgccatcg
    10381 cacggctcaa agagctgacc gacaatttcc aaccgccaga aggggcttgc ggaagctgga
    10441 cgcggcttta cgcgctggcg aaagaaatgg tggaggattt gaacgaccac attcatttgg
    10501 aaaacgatat tttgtttgcc cgcgtactcg attcttaagg gataggagtc ggatatttcc
    10561 atgccgtctg aaatacggtt ttccgtttca gacggcattt ccgtgtttca gcccagcaat
    10621 tcgggcggaa tgcggcatac gggaatcggg ttgattttgt gctgcatggc gcggtgaagt
    10681 cgcgtataga tttctagaac ttcgcgctgc cgcccttcaa aatcttcggg tttgcgcgtg
    10741 ccgtacacgc ccattgccca ctccagttcg ggatagcttg cgcccatctg ttcttcgtcg
    10801 gtgcgttccg tatcccacag gccgtcggtc ggcggggctt tttgaatcgc ctcgtccacg
    10861 cccaatgctt cggcaagccg gtaaacctgc gttttggtca ggtcggcaat cgggctgatg
    10921 tccacgccgc cgtcgccgta tttagtaaaa aagcccacgc cgaaatcttc aatcttatta
    10981 cctgtccccg taaccagcag tccgtgtatc tgcccgtagt agtacagggt cagcatacgt
    11041 aggcggcttc tggcgttggc gagggaaagc ggctgactgt caaatgccgt ctgatgagca
    11101 ccgacggttt gttcaaaggt ctggaaggtg tcggtcagat cgaccgtttg cgcgcttaca
    11161 ttggcatatt gccgttgcag attgcggatg tgcagccttg cccgctcaag ctggccgggg
    11221 tgttggcgta tcggcatatc cagaagcagc gtggggcggc cggtgcgggc ggcgagtgcg
    11281 gagacgacgg cggaatcgat gccgccggaa acgcccacga caaacccttt tgcatttgcc
    11341 cgggcggcgt attcgtccaa ccatcgggcg atatgtgtga taactgcctg cgtatccatt
    11401 tttcctcctg tcgaaaatat ccgcatcata gcctgccggc ggtgcaattt gaatagattg
    11461 caggcaggtt gatgaatatt tttcccattc cccgcttaaa aaagtcatac gggaaaaggc
    11521 gggcggcaag atggcgcgtg ttataatatt gttgacaatt taggcggccc ggtgccgcct
    11581 taggcttcaa aacatattca tattttttac tatttgattg attcgatgtc tgattttttt
    11641 cattctgacg tgttgtccgt ttccgagttg aacgcatttg ccaaaagtct tttggaaaac
    11701 caccttgccg gtttgtggat tgccggcgaa gtatccaacc tgacccgtgc cgccagcggg
    11761 cattattatt tctcgctcaa agacagccgc gcacaggtgc gttgcgcgat gttcaagggt
    11821 gcggcggtgc gtttggcgaa gcctttgaaa gaaggcgacc atatcgaggt atcaggaaaa
    11881 atcagtattt atgaagcgcg gggcgaattt cagattaccg taaacgaagt acggctcaaa
    11941 ggtttggggc agctttacga agcctatgag cggttgaagg cgcagttgca ggcggaaggc
    12001 gcgtttgcgg cggaacgcaa gaaacctttg cccgtccgtc cgcaatgtat cggcatcgta
    12061 accagtctgg cggcggcggc tttgcgcgat gtcgttacta ccttgaaacg ccgcgcaccc
    12121 gaaatccccg ttatcgttta tccgacaccc gttcaaggcg caggcagcga attgcaaatt
    12181 gcccaagcga ttaaaaccgc ctcgcaacgc gccgaatgcg atgtgttgat tgtctgtcgc
    12241 ggcggcggca gcattgaaga cttgtgggcg tttaacgaag aaccggtcgt gcgcgccatc
    12301 gaatcctgca cggttccggt cgtcagcggc gtaggccacg aaaccgattt cacgctcgcc
    12361 gatttcgtcg ccgatatgcg cgcgcccacg ccgaccggtg cggcggaact ggtcagcccc
    12421 aaccgccaag aatcgctgca ccgcctcgcc caagcccaat gccgtctgaa aaccgttttg
    12481 gaacaacgct atttcgatgc cagccaaaaa ctcgactggc tggcgcggca aatccgtcat
    12541 ccgcgccaaa aactcgacga acagcgcgct tcaatcggca agctggcgca aacgctgtct
    12601 tactcgatga cgcaaaacgt ccgtacccac accgcccgtt tcgaacgcca aacccaagcc
    12661 ctgaaacatt gccgtcctga tgtttccgtt tgcaaaaaaa acatcgaccg ctttcagacg
    12721 gccctgtcgc acgccttcca ccaactgctt acccaccgcc gccaaagcct gactgcccaa
    12781 gccgcattgc tcgaagccgt ttcgccgcag cacattctgg agcgcggctt ttccgtcgtc
    12841 aaaaacacac gcggacaagt catccgcaac gccgatgttt tgaagcaggg gcagaagctg
    12901 cacattatct tcgccgacgg cgaaaccgac gtgcgcgtaa gcaaagaaca agggcagcag
    12961 gatttgttcg attgcattta aacccaatcg gaaaaaccgc tcccccgccg ccgtcattcc
    13021 cgcgaaagcg ggaatccagt cccttgacct taagataatc tttaattcgt cattcctgaa
    13081 aaagcgggaa tccggaagcg aaaagccgca ggaatttgtc ggaaaaaaca gaaacctttc
    13141 cgtacacccg aaatgtttca gacggcgcat cttggggcgg ataaaaaccc gacccgatat
    13201 ttttttataa ttttcaataa gtttccattg ggcaaatgaa attttatttt tcaactgaca
    13261 gaaagaggat gttatgaagt ttttagaccg tgaggcaagc attgccaagc cgggtttcaa
    13321 ccgttggctc gtaccgcccg cagcattggc ggtacacctt gccatcgggc agatttacgc
    13381 ctattccgta ttcaacgcgc cgctgaccaa acttatcggc ataaccgaat cggcggccgg
    13441 agattggaag ctgacgaccg taggttggat tttcagtatc gcactcgcga tgctgggcgc
    13501 gtcggccgcc ctgttcggta cgtggatgga acgggtaggg ccgcgcaaag ccatatttgc
    13561 cgccgcctgc tgcttcagcc tgggcttctt cgtatccgca ttcggcgtgc gtacgcacaa
    13621 cctcttcctg ctctatttgg gcaacggcgt catcggcggc gtaggcttgg gtttgggcta
    13681 catcgggccg gtttccacac tgatgaaatg gtttcccgac aagccgggta tggcgaccgg
    13741 tttggcgatt atgggtttcg gtggcggcgc aatgctggcc tcgccgcttt ccgtatcact
    13801 gatgaacacc ttttcaaacg ctgcttcggt cggggttgcc gaaaccttcg ccgtattggg
    13861 tctgctttac ctcgcactga tgatgttcgg cgcgtttacc atccgcgtac ctgccgacgg
    13921 ctggaaacct gaaggctata ccgcgccgaa aacgcaaaac aagctggtca gcagcaatca
    13981 tgtcaacgtg tcccaagcca tgaaaacgcc gcagttttgg ctgttgttct gggtattgtg
    14041 cctgaacgta actgccggca tcggcgtatt gggtcaggca tccgtgatga ttcaggaact
    14101 cttttccgaa acctccatag gcaggcaggc agcagtcggc gcaggtgcgg cggcaggctt
    14161 cgtcagcctc ctgagcctgt ttaacatggg cggacgcttt ttgtggtcga gcgtttccga
    14221 caaaatcgga cgcaaaaaca cctacaccat cttcttcgta ctcggttcgc tgctgtattt
    14281 cgccgttccc tccatcggcg agggcggcag caaagccctg ttcatcatcg gcttttgcgt
    14341 gattatttcc atgtacggcg gcggatttgc cgccattccc gcctacctga aagacctgtt
    14401 cggcacctat caggtcggcg cgattcacgg acgcatcctg ctggcctggt caaccgccgc
    14461 cgtcatcggc ccggtactgg tcaattacat acgccaaagc caaatcgaca gcggcatacc
    14521 ggcggcacag gcatacagcg taaccatgta catcatggcg tgcctgctga ttatcggttt
    14581 gttgtgcaac ctcgccgtca aatccgtaca cgaaaaacac cacgaaaaag acatcaaaac
    14641 cgccgcacac agcggcaacc ccgatgacga aaccgccata tccgacgctt accttgtggg
    14701 cgaaaaagtt tccggcggcg gcatatcggt ctggtggcgt tgggcattgg ccgtcatacc
    14761 gctcgcctac ggcgtggtga tggtctttgt caaagcactc gaccttttct cctgaaaacg
    14821 cccgaataaa atgccgtctg aaacagcaga tgtttcagac ggcatttttg tgttttggcg
    14881 ggtttaggct tttttgcggc ggcgttcgcc cggcagcagc atgtccgccc atttgatgat
    14941 gttggcgact tcggcggggt tgagttcgta gaactgcccg cgtttgaggc ggttgggcag
    15001 tccgatggga ccgaagccga tgcgcacgag gcggctgacg gtgagtcctt ggctttcaaa
    15061 aatgcggcgc acttcgcggt tgcggccttc tttaatcacg acgttgtacc atttgttcgc
    15121 gccttcgccg ccttgttcgc ggatgcgttc gacttttgcc aagccgtctt cgagcatcac
    15181 gccttcttcg gtgaggacgc gcatttgttc gccggtcagc ccgcccaaga cgcgcacggc
    15241 gtattcgcgt tcgacttcga agctggggtg ggcgaaacgt tggacgagtt cgccggaggt
    15301 ggtaagaatc agaagtccgc tggtgttgat gtccaagcgt ccgatggcga cccagcggct
    15361 gctggcggcc tgcggcaggc ggtcgaatat gctgacgcgg ccttgcgggt cgtcacggga
    15421 aacgatttcg ccttcttgtt tgtaatacag gatgatgcgc ggcaggcggt ccgcccattt
    15481 gagcttgatg atgctgcctt tgacggtaac gtggtcgtcg ggggtaactt tgtcgcccag
    15541 ttgcgcggtt ttgccgttga ccgttatcca gccgttggta atccattctt ccatttcgcg
    15601 gcgcgagccg acgccggacg cggcaagcac tttttgcagg cgttcgggtt ccatgcgcga
    15661 caggtcgctg cggcgttctt tcaaatcgcg cgcgtgttcc ataatttttt ggttgggatt
    15721 gcggacgacg agttttttgg ctttggcggc gcgctgtttg gggacgtttt gaggtttgat
    15781 gccgtctgaa gctttttgtc cgtaaggttg ggaagcggtt ttgcccgttt catctttggg
    15841 acgggctttg cttttgaacg gtttggcggt tttcttggca gacggggctg cgccgtcgcg
    15901 ccattggcgt ttgctggtgg gctgtttgga catcattttt cctaactcgc ctgataaggc
    15961 ggttcttctg cggcgtgtgc ggcaggacgg gttgataaaa tgccgtcctc ggtcggacgg
    16021 ttcgatattg tacctgatgc ggcggttttg cggatgaaat cgtcgctgcg gcttggggtc
    16081 ggggcaaagt ttgtgcgacg gttgaaatcg gtttgcgtgc gggagtagaa tctttcaaat
    16141 gataagagga gcagtaaatg gatttgcaca atattcggga agattacagc aaacgggaat
    16201 tgtcggaagc cgattgcgcc gataatccga tcgagcagtt cgagcggtgg ttggacgagg
    16261 cggtacgcgc acaggtcaac gagccgacgg cggtcaatgt ggcggcggtt gacggacgcg
    16321 gcaggcccaa cagccgtatg gtgctgctga aggaagttaa ttctgaaggt tttgttttct
    16381 ttacgaatta tcacagccgc aaggggcgtt cgctggatgc tcatccattt gcggcgatga
    16441 cgtttttttg gccggagctg gagcgtcagg tgcgcgtgga agggcgtgtt gaaaggctgg
    16501 cggagaagtt gtcggacgaa tattttgaga gtcgcccgta tcagagccgt ttgggtgcgt
    16561 gggcaagtgc gcagagcgag gttattccga acaaggcggt attggtggca aaggcggcgg
    16621 cggttggact caaacatcct ctgcatgtgc cgcgtccgcc gcattggggc ggctatattg
    16681 tgattcccga tttgcttgag ttctggcagg gcagaccgag caggctgcac gaccgcatcc
    16741 aataccgttt gctggatggt ggctggatac gcgagcggct gtcgccttga acaggaaaaa
    16801 acatatgccg tctgaaaacc ggaatcgggt ttcagacggc attttttcac ggggaaaaca
    16861 cggacgggaa gttttttgtg ttctgaacgg aaagctcgaa tcctctttat accggggaat
    16921 tatgatgcgg acggcgtgaa tgcgtcggag aaaaacaagt tttccggcag cttatgctgt
    16981 tgcacaaaca gattctttgt ttgttcggtc atggccggag aaccgcaggc aaatacttcg
    17041 tattccgaca ggtcggggtg gtcttgtgcc gcgatgtctt gtacgtgacc atttcttccc
    17101 tgccagccct ctccggggcg ggacaatacg ggggtgaagc aggcgttttt cagacggcat
    17161 gccaaccctt gtgcttcttc gagggcatac aaatcatcct gatgacgcgc gccccagtag
    17221 aaatggacgg cgcggttgct gccttggcgg ataaggtcga gcaggatgct gcggatgggg
    17281 gcgtagcctg tgccggttgc cagcaggatg acgggtttgc cgctgtcttc ctgcaaggta
    17341 aacgaaccga gcgggccttt aacgcggacg atgccttttt ctttgacttt gggttcgctg
    17401 ccgaaaatca ttttcgagca gacaccgttt tcgtgcctgc ggatgtgcag ttccaaaatg
    17461 ccttcttggt cgggtaaatt ggcgatggag tagctgcggc tgacgttgcc cggcagcagt
    17521 aaatcaatgt attgcccggc gtagaaggca aacggcgggg ctttgggcag ggcaagtttc
    17581 aggagggcga catcgtgttt gaaaataata ctttcgatgc gtgcgggcag ggtgcggacg
    17641 ggtagggcat cggctttgta gccggggatg ttgatgttga tatcgctttg cgcagtggtg
    17701 cagcacatca aaatcttgcc ttgcgctttt tctgcttcgg ataaagcctg ttccgagtgt
    17761 ccgcccattt gaatatcgcc gctgaccagt tcggctttgc attgtccgca gacaccgctt
    17821 ttgcaggaat ggggcaggtt gaggttttga cgggcggcag cggtcaaaac ggtttcgccg
    17881 tcgttggcgg caaaggtggt ttggtcgggc agggtaacgg tgtggttcat gtcttggtat
    17941 ggtttgagca gatta
//
LOCUS       DAWIVB010000023        17417 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWIVB010000023 DAWIVB010000000
VERSION     DAWIVB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.023"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(127..1083)
                     /gene="gshB"
                     /locus_tag="WIX60_000548"
     CDS             complement(127..1083)
                     /gene="gshB"
                     /locus_tag="WIX60_000548"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ5304855.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALEAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELSERDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            complement(1176..2864)
                     /locus_tag="WIX60_000549"
     CDS             complement(1176..2864)
                     /locus_tag="WIX60_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ5304856.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            complement(2976..3749)
                     /locus_tag="WIX60_000550"
     CDS             complement(2976..3749)
                     /locus_tag="WIX60_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ5304857.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            complement(3792..4694)
                     /locus_tag="WIX60_000551"
     CDS             complement(3792..4694)
                     /locus_tag="WIX60_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ5304858.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            complement(4843..5559)
                     /locus_tag="WIX60_000552"
     CDS             complement(4843..5559)
                     /locus_tag="WIX60_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ5304859.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            complement(6118..6540)
                     /locus_tag="WIX60_000553"
     CDS             complement(6118..6540)
                     /locus_tag="WIX60_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ5304860.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(6658..7329)
                     /locus_tag="WIX60_000554"
     CDS             complement(6658..7329)
                     /locus_tag="WIX60_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ5304861.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPSGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(7345..7971)
                     /locus_tag="WIX60_000555"
     CDS             complement(7345..7971)
                     /locus_tag="WIX60_000555"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ5304862.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(8078..8896)
                     /locus_tag="WIX60_000556"
     CDS             complement(8078..8896)
                     /locus_tag="WIX60_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5304863.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(9155..9595)
                     /locus_tag="WIX60_000557"
     CDS             complement(9155..9595)
                     /locus_tag="WIX60_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ5304864.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHSMEGKA"
     gene            complement(9658..11064)
                     /locus_tag="WIX60_000558"
     CDS             complement(9658..11064)
                     /locus_tag="WIX60_000558"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ5304865.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEASQLSNPTVLALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHVFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHTAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            11235..12350
                     /gene="lptF"
                     /locus_tag="WIX60_000559"
     CDS             11235..12350
                     /gene="lptF"
                     /locus_tag="WIX60_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ5304866.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFAVALILLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            12347..13417
                     /gene="lptG"
                     /locus_tag="WIX60_000560"
     CDS             12347..13417
                     /gene="lptG"
                     /locus_tag="WIX60_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ5304867.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            13627..16212
                     /gene="acnB"
                     /locus_tag="WIX60_000561"
     CDS             13627..16212
                     /gene="acnB"
                     /locus_tag="WIX60_000561"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ5304868.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLASIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            16354..17349
                     /locus_tag="WIX60_000562"
     CDS             16354..17349
                     /locus_tag="WIX60_000562"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ5304869.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         3951 a         4834 c         4599 g         4033 t
ORIGIN      
        1 cgtctgaaaa cggggcggcg gggtgtccgt acggcattaa ccgcatccct gccgactgag
       61 ggaaaaccct gcctgcccga tcaaaccagg cggttgtgaa gcaaaagcct ttcagacggc
      121 atcggtttaa cgtaccgacc acgcggcaac ggcatcggca aacattgccg ccacatcgaa
      181 gcctttttgt ttcataattt cttggaatcc ggtcgggctg gttacgttga cttcggtcag
      241 gttgctgccg ataacgtcca aaccggccag caggatgccg cgccgtttga gttcgggggc
      301 gagcgtttcg gcaatttcgc ggtcgcgttc gctcaattcc tgcgccacgc cgcgcccgcc
      361 tgccgcaagg ttgccgcgtg tttcgccgtt ttgaggaata cgcgccaaag cataggggac
      421 gacttcgccg ccgataatca ggatgcgttt gtcaccgtgt acgatttcgg gaatgtagcg
      481 ttgcgccata atggtgcggg aatcaagctg catcagggtt tcgaggatgc tgccgatgtt
      541 ggggtctttt tcggtcaggc ggaagatgcc cataccgccc atgccgtcga gcggtttaat
      601 gatgatgtcg ccgtgttctt tcaaaaatgc gcggacatcg gcggaacggg tcgttaccag
      661 cgtgggcgcg gtaaagcggc tgaagttcaa aatcgccagt ttttcattga aatcacgcat
      721 cgcctgtccg ctgttaaaga ccttcgcgcc ctgctgctcc gccagcgtca gaagctgtgt
      781 ggcgtagagg tattgcatat cgaacggcgg atcggtacgc ataatcacgg catcaaatgc
      841 ttccaatgcc gtctgaactt tgtcggcggc ggcaaaccag tttttgtcgt ggtcgtcttg
      901 tgcgccgaga aactcaaaag cagacgcatc agcaaccacc agaccgtttt taaccgacaa
      961 tccgctgctc aaggtatgaa acagccgcca gccgcgtttt gccatctcgc gcatcatggc
     1021 gtaggtggtg tctttgtagg ttttgaaact tgccatcggg tcggcgataa agaggacttt
     1081 catcatattt cctttccggt gtgccgaatg tgccgcattt cgcgggcaaa ggggaaatgc
     1141 cgtccgaaca atattcagac ggcaaggatg gggttttact taggctgcca agaatctttc
     1201 agcgtgaccg tgcggttaaa caccggcgtg tctttgccgt ggtctttacg gtcggttacg
     1261 aagtagccga tacgctcgaa ctgccaacgg ctttctgccg gcaaatcttt ggcggcaggt
     1321 tcggcgtagg cggtgatttc cttgacggat tccggattga ggaaatcggt gaacggcagg
     1381 tattcgccgt cttcgccgcg cacggcatcg ggacgctcga cggtaaagag gcggtcgtac
     1441 agacggactt tgatttcggc ggcgtgttcg gcggaaaccc aatgaatcac gcctttaact
     1501 ttacggcctt ctggattttt gcccaaggtg tcgtggtcga tgctgcattt gagttcaacc
     1561 acattgcctg cttcgtcttt gacgacttca tcgcacttga tgacatagcc gtggcgcaag
     1621 cgtacttcgc cgccgggaat caggcgtttg aagcctttgg gcggattttc ggcaaagtcg
     1681 tcggcttcga tgtaaatggt ttgtgaaaca ggtacttcgc gatcgcccat ttcttcgtgg
     1741 ttcggatgga acgcggcacg gcggctttgg gttctgccgg tttcaaagtt ggtcagggtt
     1801 actttgagcg ggttcaacac cgccatcagg cgtggggcgg aattttccaa ctcttcgcga
     1861 atcgcgcctt ccaacacgct catatcgacg atgttttcag atttggaaat accggcgcgt
     1921 ttggcaaaca ggcgcagccc ttcgggcgtg tagccgcgtc ggcgcatacc ggaaatggtc
     1981 ggcatacgcg gatcgtccca gccggaaacg tgtttttcca caaccaactg attcaatttc
     2041 cgtttggagg taatggtgta caaaagctcc aagcgtgaaa actcgtattg gcgcggacgt
     2101 gtggcgtgcg gcgcggggat gttgtccaac acccagtcat acagcggacg atgggcttca
     2161 aattcgagcg tacacaagga atgcgtgatg ccttcgatgg catcggaaat acaatgcgtg
     2221 tagtcgtaca tcggatagat gcaccatttg tcgccggtgt tgtggtgatg ggcgcggcgg
     2281 atgcggtaga tgaccggatc gcgcatattg atgttgccgg atgccatgtc gattttcagg
     2341 cgcagggttt tgctgccgtc ggggaactca ccgtttttca tgcgcgtgaa caggtcgagg
     2401 ttttcttcga tactgcggtc gcgataaggg ctgtttttgc ctgcttcggt cagcgtaccg
     2461 cggtattcgc gcatttcttc tggcgtcaaa tcatcgacat acgctttgcc gtctttaacc
     2521 aaaccgacgg cgtagtcata aagctggtcg aaatagttgg aagcgaaacg cggctcgccc
     2581 gcccaatgga aaccgagcca ctcaacgtct tctttgatgg cgttgacgta ttcgtcgttt
     2641 tctttttcgg ggttggtatc gtcaaaacgc aggttgcaca agccgtcgta aatatacgcc
     2701 aaaccgaagt tcaggcagat ggatttggcg tgtccgatgt gcaggtagcc gttgggctcc
     2761 ggcggaaaac gggtttggac ggcttcgtgt ttgccgcttt tgaggtcttc ttcgatgaag
     2821 gtgcggataa aatggttgtc cgcaaattgg tctttattga gcatagtttt ctttgaacag
     2881 atggcttcag acggcattgg ggcggttcct tatgccgtct gaagcggttt gatgatgtgt
     2941 ttattgtacc cgacttgcgc gctttgacat agcgttcaga cggcatcggc aatcaggcat
     3001 tccacccccg cctctttcag catcttctgc atcgcggtat cgggcagccg gtcggtaaat
     3061 actttgtcaa acgccgtaat gtcgccgagc ctgaccagcg cgttgctgcg gaatttactg
     3121 tggtccacgc cgaggaagcg gacgcgcgca ttggcaatca tcgcctgcat cacgctgact
     3181 tctttgtagt cgtcgtccaa aagcgaaccg tcgctttcca cgccgtgcgt actcatcacg
     3241 gcataatcga ctttgaactg gttgataaaa tcgacggttg ccacgccggt aataccgccg
     3301 tccaaagggc ggacgactcc ggaagtgata atgaccgtgt aatccgtccg cgccgaaacg
     3361 atggaggcga cgtggatatt gttggtaata atccgcaggc tgccgcgccg cctgaccagc
     3421 tccgatgcca cggcttccat cgtcgtgccg atactgacaa acagcgacga accgtcgggg
     3481 atgtgttccg caatcagccg ggcaatggcg tttttttcgt tttgacaccg ggtttggcgg
     3541 tcggcgggca ggccctccgg caagtttccg cccgaagatg cgccgccgtg atggcgtttc
     3601 aggctgccgg cctcctccaa ctcgcggatg tcgcggcgta tcgtctgcgg ggtaacgtcc
     3661 aatgcggcgg caagctcgtc caccgacata aactgatgcc ggcggacaag gcttaaaatc
     3721 tctccgtgcc tttggatttt cggtttcatc gttttctgcc tccttgcatc gggatgccga
     3781 ttttaccgtg ttcagcccag cgtggaaaac accaccatca gaaacggggc ggcgatattg
     3841 accaccacgc cgaagctgac cgctaccggc acgacttcca aaccgcccgc accctgaatc
     3901 acgggcaatg taaaatccat actggtcgca ccgccaaccc ccaccgccgc atccggaaaa
     3961 cgcttcatca gcagcgggat aaatgccagt gcaaacagct ctcgtgccaa atcgttcagc
     4021 agcatgatgc tgccccatac cgcgccgtaa gcctcggtca tgaccaaacc cgagagggaa
     4081 taccaaccga agccggaagc catcgccaaa cctttcgtcc acgacacacc gtctgtcgat
     4141 gcggcaaaca gcagcccgcc cgaaagagat gaaagcataa accagaccga caaccgaata
     4201 cccctgcggt tgaccaaaac ctgccgcaac gatacgccgc tgcttttgag ctgtacgccg
     4261 atgaggaaca ccagcagcat cagacaatac atgcccgcgc tttcagacgg catccaaata
     4321 tcgcgcatca gtttgccgaa tgcaaatccg agcagcacgc atccgagctg ccccacactg
     4381 cccgacacgc cgaccgaaac gcccttccct ttccccttta tccgccacgg gaataacttt
     4441 cccaacactg ccaaagcaag caggttcgcc ccgaccgtac aaacaaacag ccacagaacc
     4501 gtcaacgcca tatcgtccaa ccgcgaaccc aaatcctcca cgcgcgacaa cgatacgccg
     4561 atcagcagca gcacggcata caccaagacc gatagcacct tatccaaagc gggcaggtaa
     4621 ggcttgggca cacggataaa aaatccggca aacatcggta tcaataccga aagcaatgtc
     4681 atcaggctgt ccatctactg ctctccttta ttgccgcatg atatgtgcgg tttaaaaatt
     4741 gccgtctgaa aattgcagat acccgcatcc atatttcaga cggcatcagg ttcgccatta
     4801 aaaaaccgcc tgaaggttca ggcggcttat ccgctccggc attcaatctt ccaaagtctt
     4861 ttccaaacgc tccatacagt tgcccaaatg gcggcgcagg attttgacca cgcggttgcg
     4921 cctgcccgcc agcagcaggt cgaggatttc gcggtgttcg gaatgcgtat gcgtattgat
     4981 ggcgtgtttt tcctcgcgat gcacgcccgc cacggcgaca atcagggaag accgcgcgca
     5041 cagcgtgttc ataatgtcga acagcacatc gttgcccacc aggcgcgcca gttcgacgtg
     5101 gaaggcattg gacaggcggt tccagccgac gcggtcgccc ctgccggaag cctcttcttc
     5161 gcgccttatc atcgcataaa gcggcttgag gcgcgtttcc aaatccggca aatctgcgag
     5221 gatgttcaaa atcatcgtct ccatttcgat gcgcgcattg aacacatcct gcatttcttt
     5281 caaatcggga acgtggacga acgcgcccct gttgggttgc aaatcgacaa tcttgtcgtg
     5341 cgccaaaagc gacagcgcgc cgcggacggt gttgcgcgaa cacaccatct ggcggcaaag
     5401 ttcggactcg gtcagctttt tgccgggcag cagcacctga tcggtaatgc cgtccaaaat
     5461 cagggcgtaa acacggaaca gctccgaatc gtgccgctct tcgagaatca gggaagacgt
     5521 ggtcggcgca tggataatgt cgtcgttttc aaagttcatg atgttttccg tatttttacg
     5581 ctttcaaatt ttttaagatg ttttaaggcg gctgtgtttc aaatcgtgtc ggggaaatta
     5641 aagcattgca caaatttatt ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
     5701 cgcagacagt acaaataatt cacttggtgc ttcagcacct tagagaatcg ttctctttga
     5761 gccaaggcga ggcaacgcca tactggtttt tgttaatcca ctataattca ataaattaat
     5821 atatggctta aaataacagg atgcccgcct tccgcccgcc cgcagaagca ggcgggtatc
     5881 attttaaaac gcggcattta aaatttgacc gaaaattgtt gacaatccgg aatcaagtct
     5941 gcacaatacc ccgacaagtc caagtattat aaaggctgaa taaagaggaa acagcaggca
     6001 gatatattcg ggaggtgcag tccgaatata tctgcttttt tatgcgcctc cggcttgcct
     6061 gccgcacctt tccccttcag acggcatcag ccgtttctcc ataatgccgc ccgatgccta
     6121 tttatctgcc ccggcaattt caaaactgtg ggtaatcttt gccgctttgc ccaacataat
     6181 cgaagccgaa cagtattttt cggcagacat ctgaacggcg cgctcaatgg ccgattcttt
     6241 caaatcatgc ccgaatactt tgaaatggat gtggatttcg gtaaacacgc gcggcgcatc
     6301 gtccgcccgt ttcgccgtaa ccgtcgcacg gcagtcagtc actttctgac gctgtttttc
     6361 ggcaatcatc accacatcga tgctcgaaca gcccgccacg cccaacagca gcatttccaa
     6421 agggctgggc ccgcgcttgg ccgcaccttc tgccgccgaa ccctccataa cgacgctgtg
     6481 cccgccttcc gtcgtgccga caaagcacat tccgtctatc cattttgatg taacctgcat
     6541 ggtgtcattc ctgaaaatag cgttaaaacc gctttgcata tggcgttatt gtaaacaatt
     6601 tcaagcggct tatgcagaaa tatggacaaa acggcaaaaa aacacttgaa aaccgattta
     6661 cggtttggcg gactggccgt tgatctgcac cgatttgagt ttcagcgtat aggttttgcc
     6721 gtcgtcggta tagccgattt gtgccggaat attgttcagg gacggtgcga agaaatacat
     6781 taccgtatcg tcgccgcgcc gcacccgata tttgacgact tcggtttcca cgccgcctat
     6841 gctgtatttt cctgtacccg ccttattcaa accgccgacg gaataaagtt ttttgccgtt
     6901 ggtgattttc agccccgagg ggagtttcgc gtcatttgcc gccaactgcc aggcaagcgt
     6961 gaacaaatcc atagccttgg ggctttgctc ggttttgctc tcgcccgctt tgccgtaggt
     7021 tacgctgccg tcggcgaatt tggcttccgc atacagtttg cccctgcgta tgtctctata
     7081 gtaggtaggg tgcagggtat tgccgacaac cgtaccgccg gactcgaaac ggatattgta
     7141 tagcggcact ttaatcgtcg aaacgatttt gtaagcattg ccgctgcgtt caaatgtcat
     7201 cgtggcggga atgccgtagc tgccggaata gtgcagcacg gcggattggg gcagccctgc
     7261 cgcatacgcg cacggcaggg cggcggacaa aatggcggcg gaaaatatat ttttaaaagt
     7321 cttcatcatt tgctcccgcc cggtttacgc cgtcaggaaa cgggcggcat cggcgttttc
     7381 cgaatttctg acgcggtttc cctcgataat caggcggccg gcggcaaaat cggcaacggc
     7441 tttcggataa agtttatgct caacagccaa aacccgggcg gcaacatcgt ctgccgtatc
     7501 gccgtcgagt atcggcacaa tcccttgcga tacaatcggg ccgcaatcca gttcggcagt
     7561 aacgaaatgg atggtgcagc cggcaacgcg gcagcccgcc tccaaagcgc gttcgtgcgt
     7621 atgaagtccg gtaaacgagg gcaggacgga cgggtgaatg ttcatcagcc tgccttcgta
     7681 atgggcacag aactcagggg tcagaatccg cataaaacct gccaaaacca ccaagtcggg
     7741 ttgatatgcg tcgatttttt ccatcatggc ggtatcgaag gcaagtcgtg attcaaagtt
     7801 tttatgattc aggctgtcgg tcggaatacc ctgaccggcc gcccattgca aaccggcagc
     7861 cgtttcgctg ttgctcaaca cggcggcaat acggacgttg ggaatggcgg cattgacgat
     7921 tgcctgcata ttgctgccgc gtccggaaat caggatgacg atgtttttca taatggtgcg
     7981 cttttgaaag ggatgccatc tgaaccgctg tttggtggtt tcagacggca tttgccgtaa
     8041 aaatgcccga aaacctgttt cgggcatgga ttcggactta atttactttt ttgatgtcga
     8101 cttgagccgg ctgcttggcg ggcgcgtttt cgggtgcgcc gattttgacc agtttcacat
     8161 caaataccaa agtggcgttc gggccgattt tgtcgcccgc accctgttcg cggtaggcaa
     8221 ggttggacgg gatgtagaac gtggcttcgc cgccttcttt cagaagctgt acgccttcgg
     8281 tccaacccgg aatcacttgg ctcaaaggga aggtgaccgg gccgccgttg gctttgctgc
     8341 tgtcgaatac cgtaccgtca atcaggcggc cttcgtattc cacggtaacg atgtcgtctt
     8401 tggtcggctg tttgccttcg ccctgtttgg tgattttgta ttgcaggccg gaagcagtgg
     8461 tcttcacgcc gtctttggcg gcattttctt tcagaaaggc ttcgcctttt tctttattgg
     8521 ccttcgcgtc cgccttgtgt ttttctacgg ctttagcctg ttgttcctga aggaatttca
     8581 tcatgacttc ctgagcctgc tcttcagtca ttttgatttc tttgccgtca tacactgcct
     8641 gcatggcttc ggtaaagact ttcaaatcga tttccgcacc ctgttccttc atttgcttca
     8701 gggagcgtcc gatgtccacg cccatcgcat agcttgcctg ctgcatcgtg ctgccgatcg
     8761 aagaggtgtc gccctgcgcg gcagaagcgg cggcaggttc ggatgcagat gcgggggcgg
     8821 cttctttttt gccgcaggcg gaaagtgcca aagcggcgga aagggtcagt gcgctgattt
     8881 tgaaaatggt gttcatgatg gatcttcgct gtcgataagg tcggaaaaac gggattatag
     8941 ccgagtttga atgtttcaac acacaggatg acacataaag cgtcaatcgt gtgttgccct
     9001 gttttggaag ggattgaacc ttccaaaata agttttgatt ctaccgcccc gagggacaga
     9061 tgtccaagtg gcggggttca accgataagg aaattttaat caaatagaat caagcctgtt
     9121 taaattttgt aaatgcggca tttcagacgg cattttatgc cttgccctcc atgctgtgat
     9181 gttcgatggc aaaaccgctt cggcggtaag cggtaaagcg ttcgcgcgcg tcggcgagat
     9241 cttccaagct gttgccgacg atttccaaaa cgcgcgcggg caagaccgga gcggtgttcc
     9301 aaaaaccgtc ggacaggttc aaaacggtca tgccttcggg aattcggggc agattgccgc
     9361 cgcaggcaag caggacggat ggttcagacg gcatggcttc ttccgtttcc caaatttcgt
     9421 gcggaatgaa actctcggcc tcgtattgcc aaagcatttt gtccaattcc tgaagctgcc
     9481 cgaacgaatc ggaccacacc agtatcctgc cgccgtcccg aatcgcacgg gcaatcaggc
     9541 ggcaggtgaa aatcggaact tgggcaacgt gcgtgtaaaa ggtggctttc ggcatattgt
     9601 ttgaacattt ggcaggataa tgccgtctga aaggcttcag acggcattgt ggcaaaatta
     9661 aagattccgc agatagttca gcagcaaggg aacgggacgg ccggtcgcac ctttttccgc
     9721 accggatttc cacgccgtac ccgcgatgtc aaggtgtgcc catggatagt cttcggtaaa
     9781 gtaggatagg aatgttgcgg cggtaatcgt gcccgcgccg ggcgtgccga tgtttggaat
     9841 gtcggcaaag ttggatttga gttggtcttt gtaggtctca aagagcggca gttgccatgc
     9901 tttgtcgtcc acgttgtagg aagcggcaag caggctgtcg atcaaatcct gattgttgcc
     9961 catcacgccg ctgacatcgt gccccaaggc aacaatacac gcgccggtca gggtggcgac
    10021 gtcgatgacg gctttgggtt tgaactgctc ggcgtaagtg agcgcgtcgc acaaaatcag
    10081 acggccttcg gcatcggtgt tcaacacttc gatggtcagc cctttcatac ttttcacgac
    10141 atcgcccggt ttgtttgccg cgccggaagg catattttca caagtggcga cgacggcaat
    10201 caggttaatc ggcagttgca gtttgacggc ggcgcagaag gtgctgatga cggttgccgc
    10261 gccgcacata tcaaacttca tttcgtccat gttcaggccg ggcttgaggg agatgccgcc
    10321 ggtgtcgaag gtaatgcctt tgccgaccaa taccacaggc gcggcttctt tgtcggctgc
    10381 accgaaatag ctcagttcga ccaaataggg ggcttccgcg ctgcctttgg cgaccgacca
    10441 aaacgaaccc atgttttctt tgatgtagtc tttttcgatg attttggcgt gcgcgcccag
    10501 tttttcggct tcggctttgg cggtgcgcgc taaaaattcg ggcgtgcatt cgttgggcgc
    10561 ggcgttgccc aagtcgcggc agaggctttg tccgtaaact tgcgcttcgg cgacgcgcaa
    10621 ggcttctttg acggcggctt cgtgcgcggt atggaacacg gcagtttcaa atttggcggg
    10681 cttggcttct tttttgtagc ggtcgaaacg gtaggcggca ttgccgaacg caatcgcaaa
    10741 cgcttcggca acggctgcag cctgcgcttc ttcaaagacg tgaacgtcca cattgaccgt
    10801 ttcctgattt tgcgcccatt tggcggcttc ggcggcggcc ttgttcaatg cggcgcggcc
    10861 ggtgcttttc agacagcata cggcaacagc ctgcaaaccg ttgcctgtcg ggatttttgt
    10921 gtcggcaaaa ttttgacctt cttcaagcga agacaaaagg gcaaggacgg tcgggttgct
    10981 cagttgcgat gcttcggtgc agacaaataa ctgtgcgcct gcctgctgtt cctgcaagat
    11041 ttcggttttt gtgctaaatt ccacgtttat tctcctgatt gagacggttg tcggtagttt
    11101 tcggacggcc tttcgctcaa aagaccgtct gaagacggct ggcacgattg taccccattt
    11161 gaagcaccgt ctgaaacctt gcgcggacaa tccgcctgcg ccgaaccgct taccgccccc
    11221 ctgaccgcga ttctatgatt tatcaaagaa acctcatcaa agaactctct tttaccgccg
    11281 tcggcatttt cgtcgtcctc ttggcggtat tggtctccac gcaggcaatc aacctgctcg
    11341 gccgtgccgc cgacgggcgt gtcgccatcg atgccgtgtt ggcattggtc ggcttctggg
    11401 tcatcggtat gacgccgctt ttgctggtgt tgaccgcatt tatcagtacg ttgaccgtgt
    11461 tgacccgcta ctggcgcgac agcgaaatgt cggtctggct atcctgcgga ttggcattga
    11521 aacaatggat acgcccggtg atgcagtttg ccgtgccgtt tgccgttttg gttgccgtca
    11581 tgcagctttg ggtgataccg tgggcagagc tacgcagccg cgaatacgct gaaatcctga
    11641 agcagaagca ggaattgtct ttggtggagg caggcgagtt caacagtttg ggcaagcgca
    11701 acggcagggt ttattttgtc gaaaccttcg ataccgaatc cggcatcatg aaaaacctgt
    11761 tcctgcgcga acaggacaaa aacggcggcg acaacatcat cttcgccaaa gaaggtaact
    11821 tctcgctgaa cgacaacaaa cgcacgctcg aattgcgcca cggctaccgt tacagcggca
    11881 cgcccggacg cgccgactac aatcaggttt ccttccaaaa actcaacctg attatcagca
    11941 ccacgcccaa actcatcgac cccgtttccc accgccgtac cattccgacc gcccaactga
    12001 ttggcagcag caacccgcaa catcaggcgg aattgatgtg gcgcatctcg ctgaccgtca
    12061 gcgtcctcct actctgcctg cttgccgtgc cgctttccta tttcaacccg cgcagcggac
    12121 atacctacaa tatcttgatt gccatcggtt tgtttttaat ttaccaaaac gggctgaccc
    12181 tgctttttga agccgtggaa gacggcaaaa tccatttttg gctcggactg ctgcctatgc
    12241 acattatcat gtttgccgtt gcactcatcc tgttgcgcgt ccgcagtatg cccagccagc
    12301 ccttctggca ggcggttggc aaaagtctga cattgaaagg cggaaaatga acctgatttc
    12361 acgttacatc atccgtcaaa tggcggttat ggcggtttac gcgctccttg ccttcctcgc
    12421 tttgtacagc ttttttgaaa tcctgtacga aaccggcaac ctcggcaaag gcagttacgg
    12481 catatgggaa atgctgggct acaccgccct caaaatgccc gcccgcgcct acgaactgat
    12541 tcccctcgcc gtccttatcg gcggactggt ctccctcagc cagcttgccg ccggcagcga
    12601 actgaccgtc atcaaagcca gcggcatgag caccaaaaag ctgctgttga ttctgtcgca
    12661 gttcggtttt atttttgcta ttgccaccgt cgcgctcggc gaatgggttg cgcccacact
    12721 gagccaaaaa gccgaaaaca tcaaagccgc cgccatcaac ggcaaaatca gcaccggcaa
    12781 taccggcctt tggctgaaag aaaaaaacag cattatcaat gtgcgcgaaa tgttgcccga
    12841 ccatacgctt ttgggcatca aaatttgggc gcgcaacgat aaaaacgaat tggcagaggc
    12901 agtggaagcc gattccgccg ttttgaacag cgacggcagt tggcagttga aaaacatccg
    12961 ccgcagcacg cttggcgaag acaaagtcga ggtctctatt gcggctgaag aaaactggcc
    13021 gatttccgtc aaacgcaacc tgatggacgt attgctcgtc aaacccgacc aaatgtccgt
    13081 cggcgaactg accacctaca tccgccacct ccaaaacaac agccaaaaca cccgaatcta
    13141 cgccatcgca tggtggcgca aattggttta ccccgccgca gcctgggtga tggcgctcgt
    13201 cgcctttgcc tttaccccgc aaaccacccg ccacggcaat atgggcttaa aactcttcgg
    13261 cggcatctgt ctcggattgc tgttccacct tgccggacgg ctcttcgggt ttaccagcca
    13321 actctacggc atcccgccct tcctcgccgg cgcactacct accatagcct tcgccttgct
    13381 cgccgtttgg ctgatacgca aacaggaaaa acgttgaacc aatgccgtct gaacctctct
    13441 tcagacggca tttgttttca ttgacacatt cccacagaca gatagccgtt ccctattaca
    13501 ttacctgtca taacagttcc atttttgtta aaactagtct atgatagcgg tacaaatatt
    13561 gtttacaata tttaacgcaa atcatttgca acccgacaaa agaaaaacag aaaaaggaac
    13621 aaagagatgt tagaagccta tcgtaaagcc gccgccgagc gcgccgccct cggcattccc
    13681 gccctccctt tgaacgcgca gcaaaccgcc gatttggttg agctgctgaa aagcccgccc
    13741 gcaggcgaag gcgagttctt ggtcgaactg cttgcccacc gtgttccgcc cggtgtggac
    13801 gatgccgcca aagtcaaagc ctcattcctg gctgccgttg ccgaaggcag cgcgtccagc
    13861 ccgctgatct cccccgaata tgcgaccgaa ctcttaggta caatgctcgg cggttacaat
    13921 attcacgcct taatcgaact cttggacgac gacaaactcg cgtccattgc tgccaaaggc
    13981 ttgaaacata cgcttctgat gttcgattcc ttccacgacg ttcaagaaaa agccgaaaaa
    14041 ggcaacaaat acgcgcaaga agttttgcaa tcttgggcag atgccgaatg gttcgcctca
    14101 cgcgccaaag ttcccgaaaa aatcaccgtt accgttttca aagttgacgg cgaaaccaat
    14161 acagacgacc tctcccccgc gcccgacgcg tggagtcgtc ccgatattcc gctgcacgcg
    14221 ctggccatgc tgaaaaaccc gcgcgacggc atcacgcccg acaaaccggg cgaagtcggt
    14281 ccgattaaat tgttggaaga actcaaagcc aaaggccatc cggttgctta cgtcggcgac
    14341 gtggtcggta ctggttcttc acgcaaatcc gcgaccaact ccgtcatttg gcataccggc
    14401 gaagacattc cgttcgtgcc gaacaaacgc ttcggcggcg tatgtttggg cggcaaaatc
    14461 gcgccgattt tcttcaatac ccaagaagat tccggcgcgc tgccgattga agtcgatgta
    14521 tctgctctaa aaatgggcga tgtcgtcgat atcctgcctt atgaaggcaa aatcgtgaaa
    14581 aacggcgaga ctgttgccga gtttgaattg aaatcacaag tattgctgga cgaagtgcaa
    14641 gccggcggcc gtatcaacct gattatcggc cgaggtctga ccgccaaagc gcgcgaagcc
    14701 ctgaaactgc ctgcctctac tgcattccgc ctgccgcaag cgcctgccga aagcaaagcc
    14761 ggtttcacct tggcgcaaaa aatggtcggc cgcgcctgcg gtctgcccga aggacaaggc
    14821 gtgcgcccgg gtacttactg cgaaccgcgt atgacgacgg tcggctcgca agacacgacc
    14881 ggcccgatga cccgcgacga gttgaaagac ttggcttgtt tgggcttctc cgccgatatg
    14941 gtgatgcagt ctttctgcca caccgccgcc tatccgaaac ctgtcgatgt aaaaacccat
    15001 aaagaactgc ccgcctttat ttccacccgt ggcggcgtgt cactgcgtcc gggcgacggc
    15061 gtcatccact cgtggctcaa ccgcctgctg ctgcccgata ccgtcggcac cggcggcgac
    15121 agccataccc gtttccccat cggtatttcc ttccccgccg gctccggctt ggttgccttt
    15181 gccgccgcaa cgggcgtaat gccgctcgat atgcccgagt ctgtattggt acgcttcagc
    15241 ggcaagctgc aaccgggcgt aaccctgcgc gatttggtga acgccatccc gctgtacgca
    15301 atcaaacaag gtttgctgac cgttgccaaa gccggtaaga aaaacatctt ctccggccgc
    15361 atcctcgaaa tcgaaggcct gcctgatttg aaagtggaac aagcctttga attgaccgac
    15421 gcatccgccg aacgctccgc cgccggctgt accgtgaagc tcaacaaaga gccgattatc
    15481 gagtacatga aatccaacgt cgtgttgatg aaaaacatga ttgccaacgg ctatcaagac
    15541 ccgcgcactt tggaacgccg catcaaagct atggaaaaat ggctggcaaa tcccgagttg
    15601 ctcgaagcgg ataaagatgc cgaatacgcc gccgtgattg aaatcaacat ggacgacatc
    15661 aaagagccga ttatcgcctg cccgaacgac ccggacgacg tgtgcttcat gtccgaacgc
    15721 tccggcacca aaatcgacga agtattcatc ggttcgtgta tgaccaacat cggccacttc
    15781 cgcgccgcct ccaaactttt ggaaggcaag gcagacaccc ccgtccgcct gtggattgcg
    15841 ccgccgacca aaatggacgc gaaacaattg tccgacgaag gacactacgg cgtactcgga
    15901 cgtgccggcg cgcgtatgga aatgccgggt tgctccttat gtatgggtaa tcaggcgcaa
    15961 gtacgcgaag gtgcgaccgt tatgtccacc tccacccgca acttcccgaa ccgtttgggt
    16021 aaaaacacct ttgtttacct cggttcggcg gaattggcag cgatttgctc caaactgggt
    16081 aaaatcccga ccgttgaaga atatcaagcc aatatcggca tcatcaacga acagggcgat
    16141 aaaatctacc gctatatgaa cttcaacgaa atcgacagct acaacgaagt agccgagacc
    16201 gtgaacgttt aatccccgtc atccgtatga agtaagggat tgaccgcaat gccgtctgaa
    16261 caaccttcag acggcattgc aacattccgc taacccttct ttccgcaaac gctgcaaata
    16321 cggcgttcac gcccccacat aaaggaaacg acagtgaacc tgaaaaaccg ccattttctg
    16381 aaacttttag acttcacgcc ggaagaaatc accgcctacc tcgaccttgc cgccgaattg
    16441 aaagccgcca aaaaagcagg gcgcgagatt cagcggatga aagggaaaaa catcgccctg
    16501 atttttgaaa aaacctctac tcggacgcgc tgcgcgtttg aagtcgccgc gcgcgatcaa
    16561 ggcgcgggag tgacttattt agagccgtcc gccagccaaa tcgggcataa ggaaagcatc
    16621 aaagacaccg cccgcgtgtt gggcaggatg tacgatgcca tcgaatatcg cggtttcggt
    16681 caggaagttg ttgaagaatt ggcgaaatac gcgggcgtac ccgtgttcaa cgggctgacc
    16741 aacgagttcc atcccacaca aatgcttgcc gacgcactga ctatgcgcga acacagcggc
    16801 aaacctttga accaaaccgc gtttgcctac gtcggcgacg cgcgttacaa catgggcaat
    16861 tccctgctga ttttaggggc aaaattgggg atggacgtgc gtatcggcgc accgcaaagc
    16921 ctgtggccgt ctgaaggcat tattgccgcc gcacacgccg ccgccaaaga aaccggcgca
    16981 aaaattaccc tgaccgaaaa cgcgcatgaa gccgtgaaga atgttgattt tattcatacc
    17041 gatgtgtggg tcagcatggg cgagccgaaa gaagtctggc aggaacgcat cgatttgctg
    17101 aaagattacc gcgttacgcc cgaactgatg gcggcatcgg gcaatccgca agtcaaattc
    17161 atgcactgcc tgcccgcctt ccacaaccgc gaaaccaaag tcggcgaatg gatttacgaa
    17221 accttcgggc tgaacggtgt ggaagttaca gaagaaatat tcgaaagccc cgccagcatc
    17281 gtgttcgatc aggcggaaaa ccgtatgcac acgattaaag cggtaatggt cgcggctctg
    17341 ggcgactgac agaactgtgc ctgtttaaat tcatccgcaa cacagatacc gtctgaacac
    17401 gatgttcaga cggtatc
//
LOCUS       DAWIVB010000024        16814 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWIVB010000024 DAWIVB010000000
VERSION     DAWIVB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.024"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            336..980
                     /gene="rplC"
                     /locus_tag="WIX60_000563"
     CDS             336..980
                     /gene="rplC"
                     /locus_tag="WIX60_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ5304870.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            980..1600
                     /gene="rplD"
                     /locus_tag="WIX60_000564"
     CDS             980..1600
                     /gene="rplD"
                     /locus_tag="WIX60_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ5304871.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1597..1911
                     /gene="rplW"
                     /locus_tag="WIX60_000565"
     CDS             1597..1911
                     /gene="rplW"
                     /locus_tag="WIX60_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ5304872.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVADVTTVTIKGKVKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1917..2750
                     /gene="rplB"
                     /locus_tag="WIX60_000566"
     CDS             1917..2750
                     /gene="rplB"
                     /locus_tag="WIX60_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ5304873.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLYKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2757..3035
                     /gene="rpsS"
                     /locus_tag="WIX60_000567"
     CDS             2757..3035
                     /gene="rpsS"
                     /locus_tag="WIX60_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ5304874.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3044..3373
                     /gene="rplV"
                     /locus_tag="WIX60_000568"
     CDS             3044..3373
                     /gene="rplV"
                     /locus_tag="WIX60_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ5304875.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3383..4075
                     /gene="rpsC"
                     /locus_tag="WIX60_000569"
     CDS             3383..4075
                     /gene="rpsC"
                     /locus_tag="WIX60_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ5304876.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4059..4475
                     /gene="rplP"
                     /locus_tag="WIX60_000570"
     CDS             4059..4475
                     /gene="rplP"
                     /locus_tag="WIX60_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ5304877.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4475..4666
                     /gene="rpmC"
                     /locus_tag="WIX60_000571"
     CDS             4475..4666
                     /gene="rpmC"
                     /locus_tag="WIX60_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ5304878.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4666..4929
                     /gene="rpsQ"
                     /locus_tag="WIX60_000572"
     CDS             4666..4929
                     /gene="rpsQ"
                     /locus_tag="WIX60_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ5304879.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5151..5519
                     /gene="rplN"
                     /locus_tag="WIX60_000573"
     CDS             5151..5519
                     /gene="rplN"
                     /locus_tag="WIX60_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ5304880.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5531..5854
                     /gene="rplX"
                     /locus_tag="WIX60_000574"
     CDS             5531..5854
                     /gene="rplX"
                     /locus_tag="WIX60_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ5304881.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5864..6403
                     /gene="rplE"
                     /locus_tag="WIX60_000575"
     CDS             5864..6403
                     /gene="rplE"
                     /locus_tag="WIX60_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ5304882.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6406..6711
                     /gene="rpsN"
                     /locus_tag="WIX60_000576"
     CDS             6406..6711
                     /gene="rpsN"
                     /locus_tag="WIX60_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ5304883.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6727..7119
                     /gene="rpsH"
                     /locus_tag="WIX60_000577"
     CDS             6727..7119
                     /gene="rpsH"
                     /locus_tag="WIX60_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ5304884.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7133..7666
                     /gene="rplF"
                     /locus_tag="WIX60_000578"
     CDS             7133..7666
                     /gene="rplF"
                     /locus_tag="WIX60_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ5304885.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGAEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7680..8033
                     /gene="rplR"
                     /locus_tag="WIX60_000579"
     CDS             7680..8033
                     /gene="rplR"
                     /locus_tag="WIX60_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ5304886.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8052..8570
                     /gene="rpsE"
                     /locus_tag="WIX60_000580"
     CDS             8052..8570
                     /gene="rpsE"
                     /locus_tag="WIX60_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ5304887.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8563..8748
                     /gene="rpmD"
                     /locus_tag="WIX60_000581"
     CDS             8563..8748
                     /gene="rpmD"
                     /locus_tag="WIX60_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ5304888.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8750..9184
                     /gene="rplO"
                     /locus_tag="WIX60_000582"
     CDS             8750..9184
                     /gene="rplO"
                     /locus_tag="WIX60_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ5304889.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9196..10506
                     /gene="secY"
                     /locus_tag="WIX60_000583"
     CDS             9196..10506
                     /gene="secY"
                     /locus_tag="WIX60_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ5304890.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10511..10729
                     /gene="infA"
                     /locus_tag="WIX60_000584"
     CDS             10511..10729
                     /gene="infA"
                     /locus_tag="WIX60_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ5304891.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10750..10863
                     /gene="rpmJ"
                     /locus_tag="WIX60_000585"
     CDS             10750..10863
                     /gene="rpmJ"
                     /locus_tag="WIX60_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ5304892.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10929..11291
                     /gene="rpsM"
                     /locus_tag="WIX60_000586"
     CDS             10929..11291
                     /gene="rpsM"
                     /locus_tag="WIX60_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ5304893.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11311..11706
                     /gene="rpsK"
                     /locus_tag="WIX60_000587"
     CDS             11311..11706
                     /gene="rpsK"
                     /locus_tag="WIX60_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ5304894.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11726..12346
                     /gene="rpsD"
                     /locus_tag="WIX60_000588"
     CDS             11726..12346
                     /gene="rpsD"
                     /locus_tag="WIX60_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ5304895.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12372..13358
                     /gene="rpoA"
                     /locus_tag="WIX60_000589"
     CDS             12372..13358
                     /gene="rpoA"
                     /locus_tag="WIX60_000589"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ5304896.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13382..13750
                     /gene="rplQ"
                     /locus_tag="WIX60_000590"
     CDS             13382..13750
                     /gene="rplQ"
                     /locus_tag="WIX60_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ5304897.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13897..14610
                     /gene="minC"
                     /locus_tag="WIX60_000591"
     CDS             13897..14610
                     /gene="minC"
                     /locus_tag="WIX60_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ5304898.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14638..15453
                     /gene="minD"
                     /locus_tag="WIX60_000592"
     CDS             14638..15453
                     /gene="minD"
                     /locus_tag="WIX60_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ5304899.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15457..15720
                     /gene="minE"
                     /locus_tag="WIX60_000593"
     CDS             15457..15720
                     /gene="minE"
                     /locus_tag="WIX60_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ5304900.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15724..16644
                     /gene="oxyR"
                     /locus_tag="WIX60_000594"
     CDS             15724..16644
                     /gene="oxyR"
                     /locus_tag="WIX60_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ5304901.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFICD"
     gene            complement(16641..>16814)
                     /locus_tag="WIX60_000595"
     CDS             complement(16641..>16814)
                     /locus_tag="WIX60_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304902.1"
                     /translation="WHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNILAYRVV
                     GSGKWRFENNACA"
BASE COUNT         4613 a         3149 c         4175 g         4877 t
ORIGIN      
        1 cacagcctca tcacccgcat cgatttcaac ctgttttgcc gctttgacga actgagcatc
       61 cccgattaca gtcatcaacc atattccggt ttgtcggaga aagatgcata cgctgtgatg
      121 accggatacc gacccgttaa aagagtccga ccctatgccg tctgaaaatt caaaacgctt
      181 cagacggcat attgaagata tttctgatat ttctgttgat atttctttga cttgtcagat
      241 ataatgccga gcttggtaca tttgtgccaa gtttaacttt gtctgaaaga caggccaatc
      301 gtagcctgtc cctttacttt aaaaggaaaa taatcatgac tttaggtctg gttggacgca
      361 aagttggtat gacccgcgtg ttcgacgaac agggtgtttc tgttccggta accgttttgg
      421 atatgtctgc caaccgcgtt acacaagtaa aatccaaaga tactgacggc tatactgccg
      481 ttcaagttac ctttggtcag aaaaaagcca atcgtgtcaa caaagccgaa gccgggcact
      541 ttgcaaaagc aggtgttgaa gccggtcgcg gtttgattga gtttgctttg actgaagaaa
      601 aactggctga attgaaagct ggtgacgaaa tcaccgtttc tatgtttgaa gtcggtcaac
      661 tggtcgatgt aaccggtacc tctaaaggta aaggtttctc cggcacgatt aaacgtcata
      721 acttcggtgc ccaacgtact tcccacggta actcccgttc tcaccgtgtt ccaggctcta
      781 tcggtatggc gcaagacccg ggtcgcgtgt tccccggtaa acgcatggcc ggccaatacg
      841 gcaacaccaa agcaactgtt caaaaattgg aagttgtccg tgttgacgca gaacgccaac
      901 tgctgttggt taagggtgct gttccgggtg cggtcaacag cgatgttgta gttcgtccca
      961 gcgtgaaagt aggtgcgtaa tggaattgaa agtaattgac gctaaaggac aagtttcagg
     1021 cagtctgtct gtttctgatg ctttgttcgc ccgcgaatac aatgaagcgt tggttcatca
     1081 gctggtaaat gcctacttgg caaacgcccg ctccggtaac cgcgctcaaa aaacccgtgc
     1141 cgaagtaaaa cactcaacca aaaaaccatg gcgtcaaaaa ggtaccggcc gtgcccgttc
     1201 cggtatgact tcttctccgc tgtggcgtaa aggtggtcgc gcgttcccga acaaacccga
     1261 cgaaaacttc actcaaaaag taaaccgcaa aatgtaccgt gccggtatgg cgactattct
     1321 gtcccaattg actcgtgacg agcgtttgtt tgcgattgag gcgttgactg ccgaaactcc
     1381 taaaaccaaa gtttttgccg aacaagtgaa aaatctgggt ctggagcaag tgttgtttgt
     1441 aaccaaacag ctcgacgaga atgtttactt ggcttcacgc aacttgccaa acgtgttggt
     1501 tttggaagct caacaagttg atccttacag cttgctgcgt tacaaaaaag taatcatcac
     1561 taaagatgca gttgcacaat tagaggagca atgggtatga atcaacaacg tttgactcaa
     1621 gtgattttgg cacctatcgt ttctgaaaaa agcaacgtat tggctgaaaa acgtaaccaa
     1681 atgacgttta aagttttggc aaatgcaacc aaacctgaaa ttaaagcggc tgttgagctg
     1741 ctgttcggcg ttcaagttgc agacgttact actgttacca ttaaaggtaa agttaaacgt
     1801 tttggtcgca ctttaggtcg tcgcagcgat gttaaaaagg cttatgtaag cttggctgcc
     1861 ggtcaagagt tggatttgga agccgctgct gcagctgcag ataaggaata aacaaaatgg
     1921 caatcgttaa aatgaagccg acctctgcag gccgtcgcgg catggttcgc gtggtaacag
     1981 aaggtttgta caaaggtgca ccttatgcac ctctgctgga aaagaaaaat tctactgccg
     2041 gtcgtaacaa caatggtcat attactaccc gtcataaagg tggtggtcat aaacatcatt
     2101 accgcgtcgt agattttaaa cgtaacaaag acggtatccc tgcaaaagta gagcgtatcg
     2161 aatatgaccc taaccgtact gcatttatcg cactgttgtg ctatgcagat ggtgagcgtc
     2221 gctacattat tgctcctcgt ggtattcaag ccggtgcagt attggtttcc ggtgctgaag
     2281 ctgcgatcaa agtaggtaac actctgccga tccgcaatat tcctgttggt acaactattc
     2341 actgtatcga aatgaaacca ggtaaaggtg cgcaaattgc acgttctgcc ggtgcttctg
     2401 cggtattgct ggctaaagaa ggcgcgtacg ctcaagtccg cctgcgctct ggcgaagtcc
     2461 gtaaaatcaa cgtagattgc cgtgcaacca tcggtgaagt cggtaacgaa gagcaaagcc
     2521 tgaaaaaaat cggtaaagcc ggtgccaatc gttggcgcgg tattcgtccg actgtacgtg
     2581 gtgttgtcat gaaccctgtc gatcacccgc atggtggtgg tgaaggccgt acgggcgagg
     2641 cccgcgaacc ggtcagccca tggggtactc ctgctaaagg ctaccgcact cgtaataaca
     2701 aacgcacgga taacatgatt gttcgtcgcc gttactcaaa taaaggttaa tttagtatgg
     2761 ctcgttcatt gaaaaaaggc ccatatgtag acctgcattt gctgaaaaaa gtagatgctg
     2821 ctcgcgcaag caacgacaaa cgcccgatta aaacctggtc tcgtcgttct accattctgc
     2881 ctgattttat cggtctgacc attgctgtgc acaacggccg cacccatgtg cctgtgttta
     2941 tcagcgacaa tatggttggt cataaattag gcgaattctc attgacccgt acctttaaag
     3001 gccacttggc cgataaaaag gctaaaaaga aataaggtga atcatgagag taaatgcaca
     3061 acataaaaat gcccgtatct ctgctcaaaa ggctcgtttg gtagctgatt tgattcgtgg
     3121 taaagacgtt gcccaagctt tgaatatttt ggctttcagt cctaaaaaag gtgccgagct
     3181 gattaaaaaa gtattggagt cagctattgc taatgccgag cacaataacg gtgcggacat
     3241 tgatgaactg aaagtggtaa ctatctttgt tgacaaaggc ccaagcttga aacgttttca
     3301 agctcgcgcc aaaggtcgcg gtaaccgcat cgaaaaacaa acttgtcata tcaatgtgac
     3361 agtgggtaac taaggaaaag ctatgggaca aaagattaac cctacaggct ttcgcctggc
     3421 ggtaactaaa gactgggctt caaaatggtt tgctaaaagc accgactttt ctactgtttt
     3481 gaagcaggat atcgatgttc gcaattattt gcgtcaaaaa ttggccaatg cttcggttgg
     3541 tcgagtggtt attgaacgcc ctgcaaaatc tgcacgcatt accattcact ccgctcgtcc
     3601 gggtgtggtt atcggtaaaa aaggtgagga tatcgaggtt ttgaaacgtg acttgcaagt
     3661 cttgatgggt gtacctgttc atgtaaatat tgaagagatt cgccgtcctg agttggatgc
     3721 tcaaattatt gctgacggta ttgcccagca gttggaaaag cgcgttcaat tccgtcgtgc
     3781 tatgaaacga gcaatgcaaa atgcaatgcg ttctggtgct aaaggcatta agattatgac
     3841 ttcaggccgt ctgaatggtg cggatattgc ccgtagcgaa tggtatcgtg aaggtcgcgt
     3901 gccactgcat actttacgtg caaatgtaga ttatgcaacc agcgaagcgc acaccacata
     3961 tggtgtattg ggtctgaaag tttgggttta tacggaaggc aatattaaat cttccaaacc
     4021 tgaacatgag agtaaacaaa gaaaggcagg tagacgtaat gctgcagcca actagactga
     4081 aataccgtaa gcaacaaaag ggtcgcaata ccggcatcgc tactcgcggt aataaggtaa
     4141 gtttcggtga gttcggcttg aaagccgtag gtcgtggtcg tttgactgcc cgtcaaatcg
     4201 aagctgctcg tcgtgcaatg acccgtcata tcaaacgtgg tggtcgtatt tggattcgtg
     4261 tattccctga taaaccgatt actgaaaagc ctattcaagt tcgtatgggt ggcggtaaag
     4321 gtaacgtgga atattacatt gccgaaatta aaccaggtaa agtgttgtat gaaatggatg
     4381 gcgttccaga ggaactggct cgtgaagcat tcgagttggc tgctgccaaa ttgcctattc
     4441 ctacaacctt tgtagtaaga caggtgggtc aataatgaaa gcaaatgaat tgaaagacaa
     4501 atccgttgag cagttgaatg cagatttgtt ggacttgttg aaagctcagt ttggcttacg
     4561 tatgcaaaac gctaccggtc aattaggcaa accaagtgaa ttgaaacgtg tacgtcgcga
     4621 tattgctcgt attaaaaccg ttttaactga aaaaggtgct aagtaatgag cgaaactaaa
     4681 aatgttcgta ctttgcaagg caaagtagta agcgacaaaa tggataaaac cgtaacagta
     4741 ttggttgagc gtaaagtaaa acatccgctg tatggtaaga ttattcgatt atctactaaa
     4801 atccatgccc atgatgaaaa taatcaatat ggaattggtg atgtggttgt tatatcggaa
     4861 tcccgtccat tgtcaaaaac taaatcttgg gttgtcagtg agctggttga gaaagcacgt
     4921 tctatttaag aattaaagca acgtgcttgg aatgggaaac gaagtattgc agcaaattta
     4981 atttgcgtgt aaacttcgtt tcctgtcttt cagtttcttc tggaagtttc ttccctttcg
     5041 gggtccaaga ctggtttact tgaaccgcaa ggtttcattt aataagcagc ggctttgctg
     5101 taagttatct gaaagtggta aattaagttg gttaatttaa aggtaataac atgattcaaa
     5161 tgcagaccat cttagatgtg gctgataact ctggtgcgcg tcgcgtaatg tgtatcaagg
     5221 tattgggcgg atctaagcgt cgctacgctt ctgttggcga tattattaaa gtggcagtta
     5281 aagatgcggc tccgcgtggc cgtgtcaaaa aaggcgatgt atataatgcg gtagttgttc
     5341 gtactgctaa gggtgtacgt cgtcctgatg gtgcgttaat taaattcgat aacaatgccg
     5401 ccgtgttact gaataataaa cttgaacctt tgggtactcg tatctttggt ccggtaaccc
     5461 gtgaattgcg tactgagcga tttatgaaaa tcgtttcatt ggcacctgaa gtattataag
     5521 gaatggcacg atgaataaaa tcattaaagg cgatagggtt gtagtaattg ctggtaagga
     5581 taaaggtaag cagggtcaag tagttcgagt gttgggtgat aaagttgttg ttgagggcgt
     5641 taatgttgta aaacgccatc aaaaacctaa tccaatgcgt ggcattgagg gcggtattat
     5701 tactaaagaa atgcctttgg atatttctaa tatcgcaatc ctgaatccgg aaactaataa
     5761 agcggaccgt gttggtatta agctgattga aaatgaaggc aaagttaaac gcgttcgttt
     5821 cttcaaatca aatggctcta tcattggggc ataaggagat aacatggctc ggttgagaga
     5881 gttttataaa gagacagttg ttcctgaatt ggttaaacaa tttggttaca aatcagtaat
     5941 ggaagtcccg cgtattgaaa aaattacctt gaatatgggt gtgggtgagg ctgttgctga
     6001 taaaaaagtt atggaacatg ctgtttccga tttagagaaa attgccggtc aaaaaccggt
     6061 tgttactgtt gcccgtaaat ctatcgcagg ttttaaaatc cgtgataact atccggttgg
     6121 ttgcaaagta acattgcgtc gtgatcaaat gtttgaattc ttggatcgtt tgattactat
     6181 tgcattacct cgcgtacgtg acttccgtgg tgtgagcggt aaatcatttg atggccgtgg
     6241 caattacaat atgggtgttc gtgagcaaat tatttttccg gaaattgaat acgataaaat
     6301 tgatgctttg cgtggtttga atattactat tactactaca gcaaaaaccg atgaggaagc
     6361 gaaagcttta ttgtcattgt ttaaatttcc gttcaaagga taatcatggc taagaaagca
     6421 cttattaatc gtgatctgaa acgtcaagct ttggctaaaa aatatgcggc taaacgcgcg
     6481 gcaattaaag cggtaatcaa tgattcgaat gcaactgagg aagagcgttt tgaggctcgt
     6541 ttgaggtttc aatccattcc tcgtaatgcg gcacctgtgc gtcaacgtcg tcgttgtgct
     6601 ttgacaggtc gccctcgtgg tactttccgt aaatttggtt tgggtcgtat taaaatccgt
     6661 gaaatcgcca tgcgtggcga aattccgggt gttgttaaag ccagctggta ataggagtaa
     6721 ttaagaatga gtatgcatga tcctatttcc gatatgttga ctcgtatccg caatgcgcaa
     6781 cgtgctaata aagcagcggt tgcaatgcct tcttcaaaat taaagtgtgc tattgcaaag
     6841 gtattgaaag aagaaggata tattgaggac ttcgcagttt catctgacgt aaagtctata
     6901 ttggaaattc aattaaaata ctatgcaggt cgtcctgtaa ttgaacaaat caagcgtgta
     6961 tctcgccccg gtttgcgtat ttataaagcg tctagtgaga ttccaagtgt tatgaatggc
     7021 ttgggtattg ctattgttag tacttctaaa ggtgtaatga ctgatcgtaa agcacgttct
     7081 caaggtgttg gtggtgagtt gttatgcatt gtagcctagt ggaggaaaag aaatgtcacg
     7141 tgtcgcaaaa aacccagtga ctgttcccgc tggtgtagaa gtaaaatttg gagcagaggc
     7201 attagttatt aagggtaaga acggtgaatt gtcttttcct ttgcattctg atgtagccat
     7261 tgaatttaat gatggcaaat tgacttttgt tgcgaataac agcagtaaac aagcaaatgc
     7321 aatgtctggt actgctcgcg cattagtcag caatatggtt aaaggtgttt cagaaggttt
     7381 tgagaaaaga ttgcaattga taggtgtggg ttatcgtgct caagcacaag gtaaaatctt
     7441 gaatctgtct ttgggttttt ctcatccgat cgtatatgaa atgcctgaag gtgtctccgt
     7501 tcaaactcct agccaaacag agattgtttt aaccggctcg gataaacaag ttgttggtca
     7561 agttgctgct gagattcgtg cgttccgtgc tcctgagcct tataaaggta aaggtgttcg
     7621 ctatgtagga gaagtagtgg taatgaaaga agccaagaaa aaataattga ggttcactaa
     7681 tggataaaca tacaacccga ctccgtcgtg cacgcaaaac ccgtgctcgt attgcggact
     7741 tgaaaatggt aagattatgt gtgttccgaa gcaataatca tatttatgct caagtaatta
     7801 gtgctgaagg tgataaagta ttggctcaag cctctacatt ggaagctgag gtgcgcggta
     7861 gtctgaaatc tggaagcaat gttgaagcag ctgcaatagt tggtaaacgt atcgctgaaa
     7921 aagctaaagc agcaggtgta gaaaaggttg cttttgatcg ttcaggtttc caatatcacg
     7981 gtcgtgtgaa ggctttggct gaagctgctc gtgaaaatgg tttaagcttc taaatatttg
     8041 gagactttca gatggcaaaa catgaaattg aagaacgcgg tgacggtctg attgaaaaga
     8101 tggtcgctgt taatcgcgta actaaagtag ttaaaggtgg ccgtatcatg gctttctcag
     8161 cactgactgt tgttggtgat ggtgatggtc gcattggtat gggcaaaggt aaatcaaaag
     8221 aagtaccagt tgctgttcaa aaagcaatgg atcaagctcg acgctctatg attaaagtac
     8281 ctttgaaaaa cggtactatt catcatgagg ttattggccg tcatggtgct actaaagtat
     8341 ttatgcagcc tgctaaagag ggtagtggcg taaaagccgg tggacctatg cgtttggttt
     8401 ttgatgctat gggcattcat aatatctccg ccaaagtgca cggatctact aacccatata
     8461 atatcgtacg tgcaacatta gatggtttgt ctaagttgca tactcctgct gatatcgcag
     8521 ccaaacgtgg cttgacagtg gaagacattt tgggagttaa ccatggctga acaaaaaaag
     8581 attagggtta cattggttaa aagcctgatt ggtacaattg aatctcatcg tgcatgtgca
     8641 cgcggtttag gtttgcgtcg tcgcgagcat acggtagagg ttttagatac ccctgaaaac
     8701 cgtggtatga ttaataaaat cagctacttg ttgaaagtgg agtcttgata tgtttttgaa
     8761 tacaattcaa cctgctgttg gtgctacgca tgctggtcgt cgtgttggac gcggtattgg
     8821 tagtggtctt ggcaaaacgg gtggtcgtgg tcataaaggt caaaagagcc ggtctggtgg
     8881 gtttcataag gtgggtttcg agggtggtca aatgcccttg caacgacgcc tccctaaaag
     8941 aggttttaaa tctttaacag catcagctaa tgcacagctt cgtttaagtg aactggaatc
     9001 aattgctgtt aatgagattg atattttggt cttaaagcaa gcgggtctga ttgcatctac
     9061 agtctctaat gttaaagtta ttgcttctgg tgaaatttct aaggcagttg ctttgaaggg
     9121 tattaaagtt accaaaggtg cgagagctgc tatcgaggct gttggtggta agattgaaat
     9181 gtaaggttta atattgtggc taatcaacaa acgtcatcag gttcatccaa atttggagat
     9241 cttaagaaac gtcttttgtt tctatttgga gcattgattg tttttcgaat tggtgcccat
     9301 atacccgtac ctggagttga tgctgttgct ttagctaaat tatacgaaag cgctggaaac
     9361 ggcatcctgg gaatattgaa tatgttttcc ggtgggtcgt tagagcgctt tagtatattt
     9421 gcaataggaa ttatgccata tatttcagct tctattattg tacagctcgc ttctgaaatt
     9481 ttgccatcat tgaaggcttt aaaaaaagaa ggggaggctg gtagaaaggt aattacgaaa
     9541 tatactaggt atggtactgt tttgttagca attcttcaaa gtctaggtgt tgcatctttc
     9601 gtatttcagc aaggaattgt tgtaacaagt tcatttgagt ttcatgtttc cacggtagtt
     9661 tctttggtaa cgggaaccat gtttcttatg tggcttgggg agcaaattac tgaaaggggt
     9721 atcgggaacg gtatttcttt aatcattacg gcaggtattg cttcaggtat tccttcgggt
     9781 attgcaaagc tggttacact gacgaaccaa ggttctatga gcatgcttac ggcgttgttt
     9841 attgtatttg gtgccttatt attaatttat ttggttgtat actttgaaag tgcacagcgg
     9901 aagattccta ttcattatgc aaaacgccag tttaatggta gggcgggtag tcaaaatacg
     9961 catatgcctt tcaagttgaa tatggctggt gttattcccc caatttttgc ttccagtatt
    10021 attctatttc catctactct tttaggttgg tttggttcgg ctgatacaaa tagtgttttg
    10081 cacaaaatag ctggattgtt acaacacggt caattgctgt atatggcttt atttgcagcg
    10141 acagttattt tcttttgtta tttttatacg gctttggttt ttagccctaa agaaatggca
    10201 gagaatttaa aaaagagtgg tgcttttgtt cctgggatta gacctggtga gcagacctct
    10261 aggtatttag aaaaagttgt attacgtttg acattgtttg gagctcttta tattacaact
    10321 atttgtttaa ttccagagtt cttaactacg gttttaaatg taccttttta tttgggtggc
    10381 acgtctttgt tgattctagt tgttgtaacg atggatttta gtacacaaat aaattcgtat
    10441 aggcttactc aacagtatga taagttaatg actcgttcag aaatgaaatc attttctcgg
    10501 aaatagaatt atggcgaaag aagatactat ccaaatgcaa ggtgaaattc ttgaaacttt
    10561 acctaatgca acatttaaag taaaacttga gaatgaccat attgtattgg gtcatatttc
    10621 tgggaagatg cggatgcatt acattcgtat ttctccggga gataaggtca cagtagagct
    10681 gacaccttat gatctaacta gggctcgaat cgttttcaga gcaagataaa ccaataaaag
    10741 gaaaataaaa tgcgtgtaca accatctgtt aagaaaattt gccgaaattg caagattatt
    10801 cgtcgaaatc gtgtagttcg tgtaatttgt actgatctcc gtcacaaaca gcgtcaaggt
    10861 taatggaata tttcttttaa tgtgattctg tgatatagtg acacactttg ccctaaaaag
    10921 gaaaaaatat ggctcgtatt gcaggggtaa atatccctaa taacgcacac atcgtaattg
    10981 gtcttcaggc tatttacggt attggtgcta ctcgtgctaa attgatttgt gaggctgcaa
    11041 atattgcgcc tgatactaaa gcaaaagatt tggacgagac tcaattagat gctttgcgtg
    11101 accaagttgc caagtatgaa gtagaaggtg atttgcgtcg tgaggtaact atgagtatca
    11161 agcgattgat ggacatgggc tgctatcgtg gcttccgtca tcgtcgcggc ttaccatgcc
    11221 gcggtcaacg cactcgtaca aatgcgcgta cccgcaaagg tccgcgtaaa gcgattgctg
    11281 gtaagaaata aattttaagg aattttatta atggctaaag caaacacagc ttcacgtgta
    11341 cgtaaaaaag tacgtaaaac cgtgagtgag ggtattgtgc acgttcatgc atctttcaac
    11401 aataccatca ttacaatcac tgaccgtcaa ggcaatgcgt tgtcttgggc tacctctggc
    11461 ggcgctggtt ttaaaggttc tcgtaaaagt acaccatttg cagcacaagt tgcagcagaa
    11521 gcagctggta aagttgccca agagtatggc gttaaaaatt tagaggttcg tattaaaggt
    11581 ccaggtccag gtcgtgaatc ctctgtacgt gctttgaatg ctcttggttt caagattacc
    11641 agcattactg acgttacccc gttgcctcat aacggttgcc gtccgcctaa aaaacgtcgt
    11701 atttaatatt ggagtgattt gaaacatggc acgttatatt ggccctaaat gtaagttggc
    11761 acgtcgcgaa ggtacggatt tgtttttgaa gagtgcgcgc cgctctttgg attctaaatg
    11821 taaaattgat tccgctcctg gtcagcatgg tgcaaaaaaa ccgcgtttgt cagactatgg
    11881 tttgcagttg cgtgaaaaac aaaaaatccg ccgtatttat ggcgtattag aacgtcagtt
    11941 ccgtcgttat ttcgcagaag ctgatcgtcg taaaggttct accggcgagt tgctgttgca
    12001 gttgctggaa tctcgtttgg ataatgtcgt ttatcgtatg ggtttcggtt ctacccgagc
    12061 tgaagcaaga cagcttgttt ctcataaggc gatagttgtg aatggacaag ttgtcaatat
    12121 tccttctttc caagtgaaag ctggtgatgt tgtctcagtt cgtgaaaaag ccaaaaaaca
    12181 ggtacgtatt caagaagcat tgggtttggc aactcaaatc ggcttgccgg gttgggtttc
    12241 tgtagatgcg gataaacttg agggtgtgtt caaaaacatg ccggatcgct cggaattgac
    12301 cggtgatatt aatgaacagc tggtggtaga gttctactct aaataatgct agctcagtga
    12361 gggacagtta aatgcagaat agcacaaccg aatttttgaa acctcgtcaa attgatgtaa
    12421 atactttttc tgcaactcgt gcaaaagtat ctatgcagcc atttgaacgt ggtttcggtc
    12481 ataccttagg taatgctttg cgccgtatct tactgtcatc catgaatggt tttgctccta
    12541 ctgaagtagc tattgccggt gtattacacg aatattctac tgttgatggt attcaggaag
    12601 atgttgttga cattttgctg aatattaaag gtattgtgtt taaactccat ggtcgtagcc
    12661 aagttcaact tgtgttgaag aaatcaggtt caggtgtcgt atctgccggt gatattgagt
    12721 tgccgcatga tgtagaaatt ctgaatcctg gtcatgtcat ttgtcatttg gctgataacg
    12781 gtcaaattga gatggaaatt aaagtagagc aaggtcgtgg ttatcaatct gtttcaggtc
    12841 gtcaggtagt tcgtgatgag aaccgtcaga ttggtgcaat ccagttggat gcgagctttt
    12901 cgcccatcag ccgtgttagc tttgaggttg aacctgcacg tgtagagcag cggacggatc
    12961 ttgataagtt ggttttggat atcgaaaccg acggttctat tgatcctgag gaagctgtac
    13021 gcagtgcggc acgtattttg attgatcaga tgtctatttt tgctgatttg cagggtacgc
    13081 ctgtggagga ggttgaagaa aaagcacctc ctatcgaccc tgttcttttg cgtccggtgg
    13141 atgatctgga attgacagta cgttcagcta attgtttgaa agctgaggat atttattata
    13201 ttggcgattt gattcaacgc actgaaaccg agcttcttaa aacgccgaat ttgggacgta
    13261 aatctttgaa tgagattaag gaagtattgg catctaaagg tttgacactg ggttctaagt
    13321 tggaagcatg gccacctgta ggcttggaaa agccttaatg aagaattaaa ggataattga
    13381 tatgcgtcat cgtaatggca atcgcaaatt aaaccgtacc agcagtcatc gtgctgcaat
    13441 gctgcgtaat atggcgaatt cattattgac tcacgaagct attgtaacaa ctctgcctaa
    13501 ggccaaggaa ttgcgccgtg tagtagagcc gttgattaca ttgggtaaaa agccgtcatt
    13561 ggcaaaccgc cgtttggcat ttgaccgtac tcgcgaccgt gatgttgtag taaaactgtt
    13621 tggcgatttg ggtcctcgtt ttactgctcg taacggtggt tatgttcggg tgttgaaata
    13681 cggattccgt aaaggtgata atgcacctct ggcactggtt gaattggttg acaaaccggc
    13741 tgctgagtaa ttttagtcat ataacgccat ctgccgaaaa gcaggtggcg ttatttttgc
    13801 aatatctgat aggtaatagg gtattggcta tcatgtttaa aatattaatt gaatagctaa
    13861 ggtttgcgcg gtaaacttac atcattaaaa aattctatga tggtttatat aatgaatgct
    13921 ttcgatataa agtcgacaaa gatggacgta ttgtctatat ctttgcatac gtcagacttg
    13981 tttgatttgg aagatgtgct ggtcaaattg ggcaagaagt ttcaagagtc tggtgttgtt
    14041 ccatttgtgc tggatgttca agagtttgat tatcccgagt ctttggatct tgctgcattg
    14101 gtttcgttgt tttcaaggca tggtatgcaa attttgggtc tgaagcattc taatgaacgt
    14161 tgggctgctg cggctatgaa gtatcatttg ctgttttgtc tgtctcattc ggaaaatgtt
    14221 aaagaactgg gtcaggttga ggtgcagaaa acggaggatg gtcagaaagc aaggaaaaca
    14281 gtattgatta catcccctgt ccgtaccggt cagcaggttt atgccgaaga tggcgatttg
    14341 attgttacgg gggcggtcag ccagggggcg gaattgattg cggatggcaa tatacatatt
    14401 tatgcgccga tgagggggcg tgctttggcc ggtgccaagg gtgatacttc tgcccgcata
    14461 tttatccact ccatgcaggc agaactggtt tctgtggcgg gtatttaccg taattttgaa
    14521 caggatttgc cgaaccatct gcacaagcag ccggtacaga tattgttgca ggataaccga
    14581 ttggttatca gtgcaattgg ctcagagtaa ttgtttgata tttaaaaagg aaatattgtg
    14641 gcaaaaatta ttgtagtaac ttcaggtaag ggcggtgtcg gtaaaacgac taccagtgcc
    14701 agtattgcga caggtttggc attacgcgga tataaaactg cggtaattga ttttgatgtg
    14761 ggtttgcgta acctcgacct cattatgggt tgcgagcgtc gtgtcgttta tgacctgatc
    14821 aatgtcattc agggggaggc gacgctcaac caagctttga ttaaagataa aaattgtgaa
    14881 aacctgttta ttttgccggc ttcccagact cgggataaag acgctttgac acgcgagggc
    14941 gtagaaaaag tgatgcagga gctgtccggc aagaaaatgg gctttgagta tattatttgc
    15001 gactctcctg ccggtattga gcagggtgca ttgatggcgt tgtattttgc tgatgaagcc
    15061 attgtaacga ccaatcctga ggtttccagt gtgcgtgact ccgacaggat tttgggaatt
    15121 ttgcaaagca aatcccataa ggcagagcaa ggcggttcgg ttaaagaaca tctgttgatt
    15181 acgcgttatt ctcccgaacg tgtggcaaaa ggcgaaatgc tgtctgtaca ggatatttgc
    15241 gatattctgc atattccttt gctgggtgtg attcctgaat cccaaaacgt cttgcaggca
    15301 tccaattccg gagaaccggt catccatcag gacagcgtgg cggcttccga ggcatataag
    15361 gacgttattg cccgtctttt gggcgagaac cgtgaaatgc gtttcttgga agctgagaaa
    15421 aaaagcttct tcaaacgtct gtttggagga taaggtatgt cattaatcga atttttattc
    15481 ggcagaaagc agaaaacggc aaccgttgcc cgcgaccgcc ttcaaatcat cattgcccaa
    15541 gagcgcgccc aagaaggtca ggctccggat tacctgccga ctttacgtaa agagttgatg
    15601 gaagtcctgt ccaaatatgt gaatgtttca ttagacaata tccgtatttc ccaagaaaag
    15661 caggatggta tggatgtgct tgagttgaac attactttgc cggaacagaa aaaggtatag
    15721 gacatgacct taaccgaatt gcggtacatc gtcgcagtcg cccaagaacg tcatttcggc
    15781 agggcggcgc ggcgttgttt tgtcagccag cccactttgt ctattgccat taagaaattg
    15841 gaagaagagc ttgccgtctc tttgtttgac cggagcagta acgatattat tacgaccgag
    15901 gcgggggaac gtatcgttgc acaggcgcgt aaggtattgg aagaggcgga gcttatcagg
    15961 catttggcaa atgaagaaca aaacgagctg gagggtgcgt tcaaactcgg gctgattttt
    16021 acggttgcgc cgtacctgct gccgaaactg attgtttcgt tgcgccgtac tgcaccgaaa
    16081 atgcctttga tgttggaaga gaattacacg catactttga ccgagtcgct caaacgcggg
    16141 gacgttgatg cgattatcgt tgccgaaccg tttcaagagc cgggcattgt taccgaaccc
    16201 ttgtatgacg aaccgttttt cgtgattgtc ccgaaagggc attcatttga ggaactggat
    16261 gccgtttcgc cccggatgct gggtgaggag caggttttgc tgctgacgga aggcaactgt
    16321 atgcgggatc aggtactctc aagctgttcc gaattggcgg cgaaacaacg tatacagggg
    16381 ttgaccaata cattgcaggg cagctcgatt aatacaatcc gccatatggt tgccagcggt
    16441 ttggcaatca gcgtgttgcc ggcaaccgca ctgaccgaaa acgatcatat gctgttcagc
    16501 attattccgt ttgagggtac gccgccaagc cggcgggtcg tattggcgta ccgccgcaat
    16561 tttgtccgtc cgaaggcgtt gtcggcgatg aaggcggcga ttatgcagtc gcagcttcac
    16621 ggggtaagtt ttatctgcga ctaggcgcag gcattgtttt caaaacgcca tttccctgag
    16681 ccgacaacac ggtatgccaa gatattgccg tcatcatcga ttttgagtat agcatcgcca
    16741 cggaaactgc cgtcctgaag atattcgact tttgcatcac tgtgaatgtt ttcatcagtg
    16801 ccgatgcaat gcca
//
LOCUS       DAWIVB010000025        16720 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWIVB010000025 DAWIVB010000000
VERSION     DAWIVB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.025"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(49..1218)
                     /locus_tag="WIX60_000596"
     CDS             complement(49..1218)
                     /locus_tag="WIX60_000596"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ5304903.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEHIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1401..1511)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1564..2460
                     /locus_tag="WIX60_000597"
     CDS             1564..2460
                     /locus_tag="WIX60_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ5304904.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WIX60_000598"
     CDS             complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WIX60_000598"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ5304905.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGVSYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLLYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WIX60_000599"
     CDS             complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WIX60_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ5304906.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGDIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4865..6817)
                     /locus_tag="WIX60_000600"
     CDS             complement(4865..6817)
                     /locus_tag="WIX60_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ5304907.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7094..7717)
                     /locus_tag="WIX60_000601"
     CDS             complement(7094..7717)
                     /locus_tag="WIX60_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ5304908.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7923..8552
                     /locus_tag="WIX60_000602"
     CDS             7923..8552
                     /locus_tag="WIX60_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ5304909.1"
                     /translation="MNLFQNAKFFTTINHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWLDAADAAASSPEEN"
     gene            complement(8673..11069)
                     /locus_tag="WIX60_000603"
     CDS             complement(8673..11069)
                     /locus_tag="WIX60_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ5304910.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11221..12336
                     /locus_tag="WIX60_000604"
     CDS             11221..12336
                     /locus_tag="WIX60_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ5304911.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12339..12938
                     /locus_tag="WIX60_000605"
     CDS             12339..12938
                     /locus_tag="WIX60_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ5304912.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12939..13586
                     /locus_tag="WIX60_000606"
     CDS             12939..13586
                     /locus_tag="WIX60_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ5304913.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13604..14149
                     /locus_tag="WIX60_000607"
     CDS             13604..14149
                     /locus_tag="WIX60_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ5304914.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14168..16501
                     /locus_tag="WIX60_000608"
     CDS             14168..16501
                     /locus_tag="WIX60_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ5304915.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAASAKQQAAAPAKQQ
                     AAAPAKQQAAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHT
                     LPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLP
                     KKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTL
                     SLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKALLQAEKDIADLGALY
                     SQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIE
                     KFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNS
                     GFGGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVL
                     TQNRKEAKIESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKI
                     NKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDI
                     PVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY"
BASE COUNT         4517 a         4757 c         3807 g         3639 t
ORIGIN      
        1 aatgccgtct gaactgttca gacggcattt ttgattttca aaccggaatt acagccccgc
       61 tgccgccctc aatgcagcag ctttgtcggt gcgctcccaa gtgaactcag gttcttcgcg
      121 gccgaaatgt ccgtaagcgg cggatttact gtaaatcggg cgcaagagat cgagcatttg
      181 gacgatgcct ttggggcgca ggtcgaaatg ttcgcgaact aaggcaatca gtttttcttc
      241 gctgattttg ccggtgccga aagtatcgat ggaaatcgaa gtcggttcgg caacgccgat
      301 ggcgtaggaa acttggattt ggcattgggt tgccaaacct gcggcgacga tgttttttgc
      361 gacatagcgg caggcgtaag cggcggaacg gtccactttg gacgggtctt tgccggagaa
      421 tgcgccgccg ccgtgcggag ccgcgccgcc gtaggtatcg acgatgattt tacggccggt
      481 caaaccgcag tcgccttgcg ggccgccgat aacgaagcgg ccggtcgggt tgatcaggta
      541 tttggtttcg tcggtcagca gttcagacgg cagaaccggt ttgatgatgt gttcgattac
      601 ggcgtttttc agctcttcgt aagcgatgga cggatcgtgc tgggtagaca ggacgacggt
      661 gtcgatgcgt tttactttgc cggtttcgct gtcgtaaacc acggtcagtt gggctttggc
      721 atcaggacgc agccaaggca ggcggccgtc tttgcgcaat tcgctttgac gctgcatcag
      781 gcggtggctg taatagatgg caaacggcat cagggtaggg gtttcgtcac aggcatagcc
      841 gaacatcaaa ccttggtcgc ccgcgccctg gttcaagtcg atgccttcgc cttcgttcac
      901 gccttgggcg atgtcggggg attgctggtc gtagtacacg ccgactgcgc agccgttggc
      961 atcaaagccc agctcggagg agttgtagcc gatgcgtttg atggtttcgc gtgcgacttt
     1021 gatgtagtct acttgggcgg tggtggtaat ttcgcctgcc aatacgcaca agcctgtgtt
     1081 gaccaaggtt tctgcggcga cacgtgcttt tgggtcttgc gccaagatgg catccaaaat
     1141 cgcatcggat acttggtcgg caactttatc cggatggcct tcggataccg attcggaagt
     1201 aaacagatat tcgctcattg agattttcct tgaaaaacaa accatcttct tcagacggca
     1261 tgttgtatga acataatgtc gacagcggga aatatagcaa aatttcccta ttcataccat
     1321 tcagttgaga aatattccca tttgaatagc actttggaat ctctgcccgt acgtttctta
     1381 caggcaaaaa aattcccgca tcaagcgggt ttggattgct ctggtgagcc acatcggctt
     1441 ttcaaccgtc caccttactt ttccttttga aaagcaggtt ggcatggaat tcccaactct
     1501 taatgcagca cgcatcgtag cagaaaaggc atattgccgc aatacttccc tttttcagac
     1561 ggcatgtttc gtttacaatt caggctgttt ccccctttgc gaaccgccat gcacatcctg
     1621 ttgaccgccc tgctcaaatg cctctccctg ctgccgcttt cctgtctgca cacgctggga
     1681 aaccggctcg gacatctggc gttttacctt ttaaaggaag accgcgcgcg catcgtcgcc
     1741 aatatgcggc aggcgggttt gaaccccgac cccaaaacgg tcaaagccgt ttttgcggaa
     1801 acggcaaaag gcggtttgga acttgccccc gcgtttttca gaaaaccgga agacatagaa
     1861 acaatgttca aagcggtaca cggctgggaa catgtgcagc aggctttgga caaacacgaa
     1921 gggctgctat tcatcacgcc gcacatcggc agctacgatt tgggcggacg ctacatcagc
     1981 cagcagcttc cgttcccgct gaccgccatg tacaaaccgc cgaaaatcaa agcgatagac
     2041 aaaatcatgc aggcgggcag ggttcgcggc aaaggaaaaa ccgcgcctac cagcatacaa
     2101 ggggtcaaac aaatcatcaa agccctgcgt tcgggcgaag caaccatcgt cctgcccgac
     2161 cacgtcccct cccctcaaga aggcggggaa ggcgtatggg tggatttctt cggcaaacct
     2221 gcctatacca tgacgctggc ggcaaaattg gcacacgtca aaggcgtgaa aaccctgttt
     2281 ttctgctgcg aacgcctgcc tggcggacaa ggtttcgatt tgcacatccg ccccgtccaa
     2341 ggggaattga acggcgacaa agcccatgat gccgccgtgt tcaaccgcaa tgccgaatat
     2401 tggatacgcc gttttccgac gcagtatctg tttatgtaca accgctacaa aatgccgtaa
     2461 cgaaaataaa aatgccgtct gaacaatttc agacggcatt ttgtcatctg acgatttccg
     2521 acagcggcca gcgcggacgg acattgaacg caccgacctc cctgcccttg cccaggtgca
     2581 tcgcaccggc aaaggcaatc atggcaccgt tgtccgtgca gtatgccgtc ggcgggaaaa
     2641 acacgctgac tttttcggac ggatgtttcg gcttgccttt gggggtcggg atttgcaccg
     2701 tcatgttgcc gaaagtttca cggagcttgc ggtttgcacc gaccccgccg gcgaccacta
     2761 cggttctgaa ccctgtctgc aacagggctt ttttcacttt cgccgccaac acatcgacta
     2821 ccgcatcttg aaacgcacgg cagatgtcgt tgcgtgtctg ctcaggaatg tcatccgccc
     2881 cgttttccgc gcgcactttc tcgacggcgg tcaatacggc ggttttcaaa cctgaaaaac
     2941 tcatctgcaa atcgtcggaa tgaatcatcg ggcgcggaaa aacaaacgct tcgaacctgc
     3001 ccgattccgc aagttccgac agtttcgcac cgcccggata cagcaagccc agcagtttcg
     3061 ccgttttgtc gaatgcctcg cccgccgcat catcgacgct ctcgcccaaa agcgcgtagt
     3121 cgcctatgcc cctgaccgcc ataatctgcg tatgcccgcc cgaaaccaac agcgcgacaa
     3181 aaggaaagtc gggtttttcc tccgccaaca gcggcgacag cagatgtcct tccaaatgat
     3241 ggacgggaat aacaggcttg tccaacgcta aagccagcgc gttggcgtag ctcgaacccg
     3301 ccagcagcgc accgcccaaa ccgggcccct gcgtaaaggc aaccgcgtca atgtcgccat
     3361 acgatacgcc cgcctgcgcc aaacagcctt ccgtcaacgg aacaaggcgg cggatatggt
     3421 cgcggcttgc cagctccggc acaaccccgc cgtattcggc gtgcattgcc atctgcgtgt
     3481 gcaggcagtg cgcccgcaat cctcgttcgg tgtcatacag cgcaacgcct gtttcgtcgc
     3541 aagaagattc gattcccaat accaacatgg tctgatgccg ttaaaaactg aaaaacgtat
     3601 tctagcggat ttcggcacga ctgccgtatc ccaaaaacgg aacatgccgt ctgaagaccg
     3661 ttcagacggc atcgtcgcac cgtatcaaag cgttccgtaa gaatgcagcc cgctcaaaaa
     3721 catattcacg ccgataaagg caaatgcggt tacgaacaaa ccgataatcg cccaccacgc
     3781 cagcactttg ccgcgccaac cggcaaccag ccgcaagtgc agccaaacgg cgtaattgag
     3841 ccagacgatg aacgcccacg tctctttcgg gtcccaactc caatagcgtc cccaagcatc
     3901 cgccgcccac agcgcaccca aaatggtggc aatggtaaag aacagaaagc cgacggcaat
     3961 cgccttatac ataacttcct caatcaacac ggattgcggc agccacgatt tcccgccttt
     4021 tccttccgca cgcagggaaa ccagttcggc aataccgagc atcgcggaaa tgcaaaacgc
     4081 gccgtaaccg ataaagtttg ccggaacgtg gattttcatc caccaagatt gcaatgccgg
     4141 aatcagcggc tggatggtat gcgcctcgcg ggacacgctg taccacaaga caaatccaac
     4201 cacgaccgcc ataaagccga acacgaagcc gcccaatttc tgtatggcga acttaccttc
     4261 ataataaaga tacatcagcg cggtaatcac caaaaacagg atgaacactt catacaggtt
     4321 ggaaaccgga atatgccccg catcgggacg gagcagatag ctttcgtgcc aacgtaccag
     4381 cagaccggta aagcctgcta cggcagacac ccatgcaaac acggttccca tacccaacag
     4441 cgtgttggtc ggcacatttt ttacgcttgc caaaaccgcg cccgaaatat aggcgaacag
     4501 ggcgaaaaag acaaaggcgc actgccacat gatcgccgac tggctgctga ggaaataccg
     4561 caacaggaaa atctctgccg atttgatgtc gcctccgtac aaaccgacgg cggcataggc
     4621 aagcaatacg cttaaaggaa caaaccagcg catcggtttg aaaaaccaac ccaaaaacac
     4681 ggcaataccg gcacttgccc acaacatgac cgtttcgtaa atgtccatat gcataccgga
     4741 acgggtctgt acgaaaaccg tagccgcaaa aaccagcacg gcaaataccc aatcccaaag
     4801 attcagattg ctgatcaaag acttctgaat cagcagctcg tgttccggaa gggttttata
     4861 gtgttcagtc atgattcaag tccttgccga gccgttgcag actctcgacg tgttttggaa
     4921 attccttctg caaatcccgt tcgctgcggg ccgaagacat ggcaaaacgg attttgccgt
     4981 ctgaaaacaa tacccacgcc cgtttttcgc gcacataaaa catcaatacc gtacccaata
     5041 ccaacagcac cgagccgaga tagaccaaaa gcgcacccgg ggaacgggtc atctgcaaac
     5101 ccgacgaacg cacctcggaa aacccatcaa gttgcagcag cataggcgcg ggatattcgg
     5161 tcaaacccgt gtacgcatcc atactgtgca gcaggaaacg attccgcgct tcatcctgct
     5221 gccattcggg caagccgtac cggcgtatgg tttcatccaa agcagcgttc atcacgccgt
     5281 aaagcatttc gtagaaatag ccctgcatct tatcctgctg ctctttcggg atattggacg
     5341 taataaattc gtccaatccc aaatagcctt tttgtgcaaa gatgttcagc gtgttttccg
     5401 cagccagcat gaattgttcg cggatttcgg caggtgcgcc tttggttgcg tcggcaacca
     5461 gacgtttgcg cccttcccca tctttcaaaa actcacgcaa tgccataaag gtgtccgctt
     5521 tcaactgctt gtccaagggg atacgcagcc agcggtattg ctgctgcaag ccgctgcgcg
     5581 tgccggtaat ccaaaaataa tcctgttcct gcaaaaccgg cagcatatag tttttatatt
     5641 cgaccgcctg ccctgccgca tcacggatac ggtaaacaat ggaagggccg atattggtgt
     5701 attttttacc ttcctgagta acggcgcgga catcgttcag cgtggatttc aggctttttt
     5761 cccgttccgc gccctcgctc atgtcctcca cattcataga agtgaactga tcgaactcaa
     5821 gacgatattt gtgtttgcca atttccaacg gaaactggtg tatggatgtt gccttcaaca
     5881 cgacaggctc gcgcgaagca tcacccaaat tccacgcctt gaatgtcaaa tccgaaccgc
     5941 cgtcggcaaa actcgcctga taaatcgtga tgccgtgcaa ggtcaaagga tggttcacgc
     6001 ggatggtgcg ctcgagtttc tcaccggttg ccttgtccgt cacttcaata tcgctggcga
     6061 aatcacgcgg catacccgta ttgtaaaaat cgatatggaa ttttttcagt ttgacttcaa
     6121 aaggcaagtc ctgaaccaat atcccgttgt cggcattcag gaaaaccaca tccgcactct
     6181 gcccctcgga aatattgacg ttgcccctaa atgagagatt ggacgcaccc aaaatacttt
     6241 cgggcttgaa atccttggca taaaccgcct gattgtccgg aacaatccga ccggtcagca
     6301 tacccagttt caacagcagg ttactgtcta tcaacccgcc caggcaaatg acaatcaaag
     6361 caacatgggc aaagatatag ccccatttgt tcattgtgcc ttttttggcg gcaatcagaa
     6421 ccgacccgtc ttcacggtta atggtttttc cctgaaaacc ttgtacttcc agataacgtt
     6481 tggcaacctc gggcgcaatt tttacatcca acagcgaaga atggcgcatc gccgccagag
     6541 atttttcttt aaccttttcc cgaaaagact tcatttcgcg ccagaacggc ggcacattgc
     6601 gaatcaggca caaactggta gaaaccacca aaaacatcat gataacgaca aaccatgccg
     6661 aagcatagac gtcatacagt cccagaaaac caaaaatctg cgcccaaaac gatccgaatt
     6721 tgaccaaata atccgtctgc ggctggtttt gctgcaacac cgtaccgata accgatgcaa
     6781 tacccagcag actgagcaaa gcgactgcaa agcgcatgga gctgaaaaaa gcgaaccacg
     6841 gacgggaaag aagtggggga gatctacggg atttactcat tgtgtgttta ttccgccatc
     6901 aggaatatgg gaaagcagaa ttgggcaaac agaaaacaac gtcccgattc tactgtcttg
     6961 atgcttttta tttcaagaca atgaagacag cctgcatcga ttccaacggt tgcgattgaa
     7021 aaaacttatc gcagaattgc ctgaagccgt ctgaaaactt ttcagacggc ctctaaaaca
     7081 gactattgcg gaattaacgc aaaccttgga taaagttggc gaccgctttc aaatcttctt
     7141 cagacatacg gtttgcaata tcttccatga tggtattttt acgctgaccg gacttgtagg
     7201 cattcatctg ttcaacaata tatgcctgat gctgaccgcc caaacgcgga taagcctgaa
     7261 tttcgcttcc gcctcccggc atacccgcac cgctcggacc gtggcaggac atacacgccg
     7321 gcactttttt atcgctcaaa ccgccgcgat agattttcgc acccaattcg ggattttcct
     7381 taggattggc ttcaccggat ttgggctgct gtttggcata gaatgcggat acgttcaaaa
     7441 tatcctgatc gctcaaattc attaccaccg gtttcatcac agctgccgaa ccgtgggtgc
     7501 gtttaccgtc gcggatgccg atagtttgat gatagatgta agcagtatgc tgtgccgcca
     7561 aacgcggata catcgcaatg ccgctgttac cgtctgctgc atggcaagcc gcacaaaccg
     7621 ttgcggcaac ctgtttgcct ttttccacgt ctgctttggg agaggcggaa accgcaccgg
     7681 cagccaaaac aaaggccaat aaagtcaatc gtttcatgga gtgctcctga ttacagcatt
     7741 ggataacgca acaatgctct ttttatattc aaatacggga tttttgaccc gattaaaacc
     7801 gatgattctg taaacgtgtt attctatact aaatttacat taaattacca ctgtgtttca
     7861 cataaaacca accgcatatt tttgctgtcg gacaaacggc ggcggaaaac aaggatatgc
     7921 ccatgaacct ttttcaaaac gccaaattct tcacgacgat caaccacctt aaagacctgc
     7981 ccgacacccc tctcgaaatt gcctttgtcg gcaggagcaa tgccggaaaa tccagtgcca
     8041 tcaataccct gaccaaccat gtccgtcttg cctacgtttc aaaaacaccc ggacggacgc
     8101 agcatatcaa cttcttcgag ctgcagaacg gcaattttat ggtcgatttg cccggctacg
     8161 gttatgccca agtccccgaa gcagtacgcg cacattgggt caatctgctc ggcgactatc
     8221 tgcaacagcg caaacagctt atcgggctgg ttttgattat ggatgcccgc catcctttaa
     8281 aagaactcga catccgtatg ctggattttt tccacacgac cggcagaccg gttcacatcc
     8341 tgctgtcaaa agccgacaaa ttatccaaaa acgaacagat aaaaaccctg tcccaagtca
     8401 aaaaactgct caaaccttat tccgacaggc aaaacatcag cgtacagctg ttttccagcc
     8461 tgaaaaaaca aggtattgac gaggccaacc gaactgtcgg aagctggttg gacgcagcag
     8521 atgccgccgc ttcctctcca gaggaaaact gaccccaatt atacggaaac cgtattcccc
     8581 ccacttgacc gaccgcaaac atttaaaaaa ttgccactgc caaatctaaa atgccgtctg
     8641 aaaagtcttt cagacggcat tttgcggagt ctttaaaaca gagaatccaa ctgctgctgt
     8701 ttggaaccag tattactcgg aagcaccggc gtttcctgca tatcttggcg gacttcgtca
     8761 tccgccgcct gccgtccgcc ttctgccgcg cccccgtcat cttcttttgc ccgtcgggaa
     8821 ggttgcggcg caataccgct gttgtccagc gtcaagcccg gatcggttac catacgttcc
     8881 ttcatatagt attcgccatt gctgctgacc acaccttcag gcattttcat ccccttgccc
     8941 tgctttcctt tcaacgcaaa acgcatatag tccacccaaa ccggcaccgc aatcgtaccg
     9001 ccgtagccga cacgccccat actcttaggt ttgtcgaagc cgatatatac ggcagtaacc
     9061 acatcagggt taaaaccgac aaaccacgca tccttattgt cattggtcgt acccgtttta
     9121 ccggcaatat ccgttcttcc caacgcagct gccccccttg ccgtaccaac acggaccaca
     9181 tcctgcataa tcttatacat aatataggca ttgcgcggat cgattgcctg aggcgcattt
     9241 tgcccagcca ccaaaggttg catttgggcg cgcaacctgc cgtctctgtc ataaatctta
     9301 tcgattacgt gcgaagaaac cctatatccg ccgttcgcaa atacgctata tgcctccgcc
     9361 actttcaacg gcgttgtctc gcccgtacct aaagccatag acaggcttgc cggcagctcg
     9421 gacgacctga agccgaaacg ccggatatac tgttgcgcgt aaccgacacc gatagacatc
     9481 aaaatacgga tggaaaccat attcttggaa gccgtcagag cctgtctcaa agtaatgtag
     9541 ccggaatatc tgccgtctga atttttaggt gtccaaaccg aaccgttcgg ccctttcccc
     9601 ggcagggaaa tcggcgcatc gttaaccact gtggacgcgg tcatcccctt agataatgcc
     9661 gccgaataga caaacggctt aaaggtcgaa cccggctgcc gcattgcctg aacggcacga
     9721 ttgaatgttt tgctgtgaaa atcataaccg ccgaccagcg cgcgcacagc tccggttttt
     9781 gcatccagcg aaaccaaagc cccctgcagc aacggctctt gaaccaccgc ccaacgcccg
     9841 ccgttgtttt tgacacggat gaccgcgccc ctgcggatac ggtcctcccc cattttttca
     9901 ttattgaccg cgcgggccgc aaaacccaag gcgcgcctgt caagcgtaac ccgcctgccg
     9961 ccgggcagct gtatgacgac atttttcttt ttagtcacat ccaacacaac ggcgggaacc
    10021 attttatcga cggtatagag tcccgacaga tactggctga cagtctcctc gacatcttca
    10081 ctcttactca aatcgatata gttttccgca ccgcggtagc tgctgccgcg atcgaaattc
    10141 cgtagagcct tgcgcaatgc ctcggttgcc accttctgat gatcggcgcg gaccgtggta
    10201 taaaccttaa aaccctgcgt ataggcatct tcaccgtatt tctcatacag ttcctgacgc
    10261 accatttccg ccacatataa cgcactctga tcgattttcc gaacaaaccg ctcgtaatgc
    10321 agttcctcat tcaacgcctg atcgcgctgt tgcacggtaa tcatcttctc ctcgagcata
    10381 ttgttcaaaa tatacttctg gcgcaacttg gcacgttctg gattaacaat cggattatag
    10441 gcagacggag ccttgggcag tcccgcaagc atggcggctt ccgccaaagt caaatctcgg
    10501 acattcttat tgaaatagat ttgcgcggca gatgcaaaac cataggcgcg ctgaccgagg
    10561 taaatctgat tgaaatacaa ctcgaggatt ttgtctttgc ttaaagactg ctcgatttta
    10621 taggcaagca acacctcatt gaatttgcgt gtgaacgttt tttcactgct caaataaaaa
    10681 tttttcgcca cctgctgcgt aatcgtactc gcacccgact gcacgctgcc ggacacgaca
    10741 ttgccgacgg cagcgcgggc aacaccccaa acatccaccc cccaatgccg gtaaaagcgt
    10801 ttatcctcgg cggcgataac cgcattccgc aacacctctg ggaaatcgcc gatttttgta
    10861 aattcgcgcc gctgctcccc atacataccg atgacttccc catccgccga ataaatagtc
    10921 aacggcattt taggctggta atgctgcaaa gaatccaaag acggcagttt cggatacgtt
    10981 accaaaatag caatggcaac caaacccact ccaaatacac aaaacccaaa aaccaaacca
    11041 aaacaagtcg ttaaaatctt tttaatcata gctgaataat aatttaccat tattggtatt
    11101 aaataaagta aaatagcaac cgatttctac aaagcacggt ttcaatgtgc aaagaacaag
    11161 gaatccatta cggataccga aacggttact cactgtacaa ataaagcagg aactttcatc
    11221 atgcgcttgt ttaaaagctt gaaaaaccct aaaaaaacag atgccaagct ccctaaaaaa
    11281 tcttcgggac tcaataaccg cgcggcaatc ggcatcgata tcgaccagca ttccatcaaa
    11341 atggtccaat tgtcaggacg tagtttaaac caaattcaat tggaaaaata cgtcattgcc
    11401 aaattaccaa agaatatcat tcaaggcaat aaagtccaaa attacgatca acttgttaca
    11461 tatttgcaac aagcctatgc caaactgggt acttcgtgca aaaacatcat cgcgtccgtc
    11521 ccgcaaaatt tggcaaccat cgaacaattg acctacacag acaaagatgc agaattagac
    11581 ctgcaggggt tcgtggagtc ctccatctcc gaagtcagct cgatatcgct cgaagaagcc
    11641 aattacgact atcaggtctt gtcccaatcg gccgccggcg aagctgtgtt ggccgtcgca
    11701 tcgagaaagg atgaaatcga acccctgatt gacgcattca acgccgccgg tatgaaatta
    11761 tccgcgcttg atgtggacat tttcggacaa tacaacgcct acgcgctatg gataaaccat
    11821 ttcgcccccg agcttgcagc cgaaaaagtc gccattttcg gcgtatatgc cgcacagacc
    11881 tacgccttgg tcatccaaga cggaaaaatc ctatacaaac aggaaacctc cgtcagcgaa
    11941 gaacagctca accaactcat ccagcgcacc tatcaggtaa cagaagaaaa agcggaagaa
    12001 atcatcaact ccccgcaaaa accttccgat taccaagaaa gcgtggcaaa ctatttcaac
    12061 cagcagatta cccaagaaat acaaagggtc ttgcagtttt attacaccac gcagaccgca
    12121 gacgatatga ccgacatcaa gcatatcctg ctgaccgggg aagcggcgcg ccaggaaggc
    12181 atcgcccaaa ccgtcgcctc acaaaccaat gcagatgtac aatgcgtcca tcccgcgcgt
    12241 tattttgcgg acaacctcaa aacagacaaa caacaattcg aacttgatgc gccgacactg
    12301 accagggcgt tcggtttggc ggtacgggga ttataattat gaacaattta atcaaaatca
    12361 acctcctccc ctacagggaa gagatgaaca agcgcaaaca gcagcagttt aaaacgctga
    12421 tgtacggtgc cgtgctgacg ggcgttgccg ccgttgcggc aacctacctg tttatcgaca
    12481 atatgatcaa taaccagtcg gaaagaaaca cgctgctgga aacctccatc gcacacttgg
    12541 ataccgagct gtcggaaata caaaagctca aacaggaaaa agatgccttc ctgattaaga
    12601 aaaacaaaat cgaggagctc cagctcaaac gcctccaagc cgcaaaaatc ctcgacagcc
    12661 tgaatgaggc cgtccccgga agcacctacc tgacctcgct ggatgccgtt accgccgact
    12721 cttatcggct cagcggcagg acatccagcg acaaccgcgt tgccgccatg atgagggcga
    12781 tgcccaatac cggcatattc aagcaacccg aattgttaag catcaagaaa aacaattcgc
    12841 atcaagaatt tacccttcag gcaacattac aacccatcgt aaaggcggcc gaatccaaag
    12901 agaatccggc ttcgggaaac gcacaggagg caaactgaat ggcttctaaa tcatctaaaa
    12961 ccaacttgga tctcaacaac cttcacctgc tcaaccttcc tgccaggctt tttatcgccc
    13021 tgctggccgt tgccgccgtg ctggggctcg gttatgccgg attgttcaaa agccagatgg
    13081 aatcccttga ggaatacgaa gcaaaagaaa ccgaactgaa aaacacctac aaacagaaaa
    13141 gtatcgacgc ggccagcctg aacaacctga gggacgaact tgcctcaatc cgctctgcct
    13201 tcgatatcat gttgaaacag ctgccgacag atgcagaaat tcccaatctg gttcaagagc
    13261 ttcatcaggc aggttcgagc aacggtctgc gcttggacag cgttatgccc caacctcccg
    13321 tagatgacgg ccccatcaaa agattaccct attccatttc cattaccgga aattacgaac
    13381 agatcagcca atttacccgc gatgtcggca gcctctcccg aatcattacc cttgagtcgc
    13441 tgaaaatcgc ccaatctccg gaaaacggcg gcaatcctga cggcaagagc agcatcctga
    13501 acctcagcgc cattgccacc acctaccaag caaaatccgt agaagagctt gccgcagaag
    13561 cggcacaaaa tgccgagcaa aaataactta cgttagggaa accatgaaac actatgcctt
    13621 actcatcagc tttctggctc tctccgcgtg ttcccaaggt tctgaggacc taaacgaatg
    13681 gatggcacaa acgcgacgcg aagccaaagc agaaatcata cctttccaag cacctaccct
    13741 gccggttgcg ccggtataca gcccgccgca gcttacaggg ccgaacgcat tcgacttccg
    13801 ccgcatggaa accgacaaaa aaggggaaaa tgcccccgac accaagcgta ttaaagaaac
    13861 gctggaaaaa ttcagtttgg aaaatatgcg ttatgtcggc attttgaagt ccggacagaa
    13921 agtctccggc ttcatcgagg ctgaaggtta tgtctacact gtcggtgtcg gcaactattt
    13981 gggacaaaac tacggtagaa tcgaaagcat taccgacgac agcatcgtcc tgaacgagct
    14041 aatagaagac agcacgggca actgggtttc ccgtaaagca gaactgctgt tgaattcttc
    14101 cgacaaaaac accgaacaag cggcagcacc tgccgcagaa caaaattaag aagaggatta
    14161 ctccattatg aataccaaac tgacaaaaat catttccggt ctctttgtcg caaccgccgc
    14221 ctttcagaca gcatcggcag gaaacattac agacatcaaa gtttcctccc tgcccaacaa
    14281 acagaaaatc gtcaaagtca gctttgacaa agagattgtc aacccgaccg gcttcgtaac
    14341 ctcctcaccg gcccgcatcg ccttggactt tgaacaaacc ggcatttcca tggatcaaca
    14401 ggtactcgaa tatgccgatc ctctgttgag caaaatcagt gccgcacaaa acagcagccg
    14461 tgcgcgtctg gttctgaatc tgaacaaacc gggccaatac aataccgaag tacgcgggaa
    14521 caaagtttgg atattcatta acgaatcgga cgataccgtg tccgcccccg cacgccccgc
    14581 cgtaaaagcc gcgcctgccg caccggcaaa acaacaggct gccgcaccgt ctaccaagtc
    14641 cgcagtatcc gtatccgaac cctttacccc ggcaaaacaa caggctgccg caccgtttac
    14701 cgagtccgta gtatccgtat ccgcaccgtt cagcccggca aaacaacagg cggcggcatc
    14761 agcaaaacaa caggcggcgg catcagcaaa acaacaggcg gcagcaccag caaaacaaca
    14821 ggcggcagca ccagcaaaac aacaggcggc agcaccagca aaacaaacca atatcgattt
    14881 ccgcaaagac ggcaaaaatg ccggcattat cgaattggct gcattgggct ttgccgggca
    14941 gcccgacatc agccaacagc acgaccacat catcgttacg ctgaaaaacc ataccctgcc
    15001 gaccacgctc caacgcagtt tggatgtggc agactttaaa acaccggttc aaaaggttac
    15061 gctgaaacgc ctcaataacg acacccagct gattatcaca acagccggca actgggaact
    15121 cgtcaacaaa tccgccgcgc ccggatactt taccttccaa gtcctgccga aaaaacaaaa
    15181 cctcgagtca ggcggcgtga acaatgcgcc caaaaccttc acaggccgga aaatctccct
    15241 tgacttccaa gatgtcgaaa tccgcaccat cctgcagatt ttggcaaaag aatccggaat
    15301 gaacattgtt gccagcgact ccgtcaacgg caaaatgacc ctctccctca aggatgtgcc
    15361 ttgggatcag gctttggatt tggttatgca ggcgcgcaac ctcgatatgc gccagcaagg
    15421 gaatatcgtc aacatcgcgc cccgcgacga gctgcttgcc aaagacaaag ccctcttaca
    15481 ggcagaaaaa gacattgccg atttgggtgc gctgtattcc caaaacttcc agttgaaata
    15541 caaaaatgtg gaagaattcc gcagcatcct gcgtttggac aatgccgaca caaccggaaa
    15601 ccgcaatacg cttgtcagcg gcaggggcag cgtgctgatc gatcccgcca ccaataccct
    15661 gattgttacc gatacccgca gcgtcatcga aaaattccgc aaactgattg acgaattgga
    15721 cgtacccgcg caacaagtga tgattgaggc gcgtatcgtc gaagcggcag acggcttctc
    15781 gcgcgatttg ggcgttaaat tcggcgcgac aggcaagaaa aagctgaaaa atgatacaag
    15841 cgcattcggc tggggggtaa actccggctt cggcggcgac gataaatggg gggccgaaac
    15901 caaaatcaac ctgccgatta ccgctgccgc aaacagcatt tcgctggtgc gcgcgatttc
    15961 ctccggtgcc ttgaatttgg aattgtccgc atccgaatcg ctttcaaaaa ccaaaacgct
    16021 tgccaatccg cgcgtgctga cccaaaaccg caaagaggcc aaaatcgaat ccggttacga
    16081 aattcctttc accgtaacct caatcgcgaa cggcggcagc agcacgaaca cggaactcaa
    16141 aaaagccgtc ttggggctga ccgttacgcc gaacatcacg cccgacggcc aaatcattat
    16201 gaccgtcaaa atcaacaagg actcgcctgc gcaatgtgcc tccggtaatc agacgatcct
    16261 gtgtatttcg accaaaaacc tgaatacgca ggctatggtt gaaaacggcg gcacattgat
    16321 tgtcggcggt atttatgaag aagacaacgg caatacgctg accaaagtcc ccctgttggg
    16381 cgacatcccc gttatcggca acctctttaa aacacgcggg aaaaaaaccg accgccgcga
    16441 actgctgatt ttcattaccc cgaggattat gggtacggcc ggcaacagcc tgcgctattg
    16501 atgcgtcaaa ataagggcat atgttttacg gcatatgccc tttctttatg ctttttgccg
    16561 cgaccgaaat gccgtcattc ccgcgcaggc agaaatccaa cttgtccggt tccggttgtt
    16621 tttcgtctcg taacttttga gccgtcattc ccgcgaaagc ggaaatccag tccgttcagt
    16681 ttcggtcatt tccgataaat tcctgttgct tttcatttct
//
LOCUS       DAWIVB010000026        16488 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWIVB010000026 DAWIVB010000000
VERSION     DAWIVB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.026"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..120
                     /locus_tag="WIX60_000609"
     CDS             <1..120
                     /locus_tag="WIX60_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ5304916.1"
                     /translation="NVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            127..202
                     /locus_tag="WIX60_000610"
     tRNA            127..202
                     /locus_tag="WIX60_000610"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:160..162,aa:Trp,seq:cca)
     gene            317..595
                     /gene="secE"
                     /locus_tag="WIX60_000611"
     CDS             317..595
                     /gene="secE"
                     /locus_tag="WIX60_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ5304917.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            597..1133
                     /gene="nusG"
                     /locus_tag="WIX60_000612"
     CDS             597..1133
                     /gene="nusG"
                     /locus_tag="WIX60_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ5304918.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1234..1668
                     /gene="rplK"
                     /locus_tag="WIX60_000613"
     CDS             1234..1668
                     /gene="rplK"
                     /locus_tag="WIX60_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ5304919.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1668..2363
                     /gene="rplA"
                     /locus_tag="WIX60_000614"
     CDS             1668..2363
                     /gene="rplA"
                     /locus_tag="WIX60_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ5304920.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2593..3093
                     /gene="rplJ"
                     /locus_tag="WIX60_000615"
     CDS             2593..3093
                     /gene="rplJ"
                     /locus_tag="WIX60_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ5304921.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3151..3522
                     /gene="rplL"
                     /locus_tag="WIX60_000616"
     CDS             3151..3522
                     /gene="rplL"
                     /locus_tag="WIX60_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ5304922.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3712..7890
                     /gene="rpoB"
                     /locus_tag="WIX60_000617"
     CDS             3712..7890
                     /gene="rpoB"
                     /locus_tag="WIX60_000617"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ5304923.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDFGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            8047..12222
                     /gene="rpoC"
                     /locus_tag="WIX60_000618"
     CDS             8047..12222
                     /gene="rpoC"
                     /locus_tag="WIX60_000618"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ5304924.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            12514..12771
                     /locus_tag="WIX60_000619"
     CDS             12514..12771
                     /locus_tag="WIX60_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304925.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMRMPINIG"
     gene            12768..12917
                     /locus_tag="WIX60_000620"
     CDS             12768..12917
                     /locus_tag="WIX60_000620"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ5304926.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            13096..13467
                     /gene="rpsL"
                     /locus_tag="WIX60_000621"
     CDS             13096..13467
                     /gene="rpsL"
                     /locus_tag="WIX60_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ5304927.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13585..14055
                     /gene="rpsG"
                     /locus_tag="WIX60_000622"
     CDS             13585..14055
                     /gene="rpsG"
                     /locus_tag="WIX60_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ5304928.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14074..16179
                     /gene="fusA"
                     /locus_tag="WIX60_000623"
     CDS             14074..16179
                     /gene="fusA"
                     /locus_tag="WIX60_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ5304929.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            16265..>16488
                     /locus_tag="WIX60_000624"
     CDS             16265..>16488
                     /locus_tag="WIX60_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ5304930.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILSKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETR"
BASE COUNT         4403 a         3664 c         4248 g         4173 t
ORIGIN      
        1 aacgtaacca ttaccgtaga actgattgcg cctatcgcta tggaagaagg cctgcgcttt
       61 gcgattcgcg aaggcggtcg taccgtgggt gccggcgtgg tttcttctgt tatcgcttaa
      121 gtttagaggc caatagctca attggtagag tatcggtctc caaaaccgag ggttgggggt
      181 tcgagaccct cttggcctgc caaataaaaa attaaccggc cttgtgtcgg ttaatttttt
      241 tgtatttgtt atttagtaaa ctctcttgcc atttacatgg attgagaata gacagatgct
      301 atgatggata aataatatga cagaacatac gcctgaaaaa aagaacgtta aagtggatca
      361 actggttgtt caagataaag aatctgcatc taattccggt aaggaagggt tttttgcata
      421 tttctcaaat tcttggtccg aattcaaaaa ggtggtttgg cctaagcgtg aagatgctgt
      481 cagaatgact gtatttgtta tagtgtttgt tgctgtgctt tctatattta tctatgcggc
      541 agatacagca atttcgtggt tattttttga tgtattgctg agaagggaag gttgagatgt
      601 cgaaaaaatg gtatgttgta caggcgtatt cggggtttga gaagaatgtc caacgaatat
      661 tggaagagcg cattgcccgt gaggagatgg gagattattt cggacaaatt ctggtgcctg
      721 tagagaaagt tgttgatatc cgcaatggtc gtaagactat tagtgaaaga aagtcatatc
      781 ctggttatgt gctagttgag atggaaatga cagatgactc ttggcatctt gtaaaaagca
      841 ccccccgtgt ttccggtttt attggaggga gggctaatag acctacgccg attagtcaga
      901 gagaggctga aattatttta cagcaggttc agaccggcat agagaagccg aaaccaaaag
      961 ttgaatttga ggtcggtcaa caggttcgtg taaatgaagg gccgtttgcg gattttaacg
     1021 gggtggttga ggaggtcaat tatgaacgga ataagttacg cgtgtctgtt cagatatttg
     1081 gtagagaaac acccgttgag ctggagttca gccaggttga aaagattaac tgatttttat
     1141 acttgaaaaa aaagcaataa gaggatagaa tcaaaaatta acttggggag cggaaatggt
     1201 tccgcgtctt acccgttttt aggagttcgt taagtggcaa agaaaattat cggctatatt
     1261 aaactgcaaa ttcctgcagg taaagccaat ccatctcctc cggttggtcc tgctttgggt
     1321 cagcgcggtt tgaatattat ggaattttgt aaggcattta atgctgcaac ccaaggtatg
     1381 gagcctggct taccgattcc ggttgtgatt actgcatttg cagataaatc attcacattt
     1441 gtgatgaaaa ccccgccagc ttctatcttg ttgaaaaagg ctgccggttt gcaaaaaggt
     1501 agttctaatc ctctgaccaa caaagtgggt aaattgaccc gtgcccagtt ggaagaaatt
     1561 gctaaaacta aagatcctga tttgactgct gctgacttgg atgcggctgt ccgtactata
     1621 gcaggttctg ctcgctcaat gggcttggat gtggagggtg ttgtataatg gctaaagtat
     1681 ctaaacgctt gaaagctctt cgctcttctg tggaagccaa taaattatat gcaattgatg
     1741 aagcaattgc tttggtaaaa aaagcagcga ctgctaaatt tgacgagtct gttgacgtat
     1801 ctttcaactt gggcgttgat ccgcgtaaat ctgaccaagt tatccgtggt tcggtcgttc
     1861 tgcctaaagg caccggtaag ataacccgtg tggctgtatt tactcaaggt gcaaatgcag
     1921 aagctgctaa agaagctggt gcagatatcg tcggtttcga agatttggct gctgaaatca
     1981 aagcaggcaa tctgaacttt gatgtcgtta ttgcttctcc cgatgcaatg cgtattgttg
     2041 gtcagttggg tactattttg ggtcctcgag gcttgatgcc aaaccctaaa gtaggtacgg
     2101 ttactcctaa cgttgctgaa gcagttaaga atgcaaaagc aggtcaagta caataccgta
     2161 cagataaagc aggtatcgtt catgcaacga ttggtcgtgc ttctttcgct gaagctgatt
     2221 tgaaagagaa ctttgatgcg ttgctggatg ctatcgttaa agccaagcct gctgccgcta
     2281 aaggtcagta tctgaaaaaa gttgctgtgt ctagcaccat gggtttgggt attcgcgttg
     2341 atacatcaag cgtaaataac taatcttaag gaattttcaa gcagtttggt tttctgggct
     2401 gcttgaattt gggctactta aaattaagta gatgtccaag accgtaggga tcgtaagatt
     2461 taatcgtaac tgccctacgc agacggtagt cctgaaacac attgcaagat tgcttgtaag
     2521 atgtcttttt aggttaccgc gctggtggga tatcgttttg gtatcctgtt tataaacagt
     2581 gggaggtaga ccttgagtct caatattgaa accaagaaag tggcggtcga ggaaattagc
     2641 gcggcaattg ctaatgctca aaccctcgta gtcgctgaat atcgcggtat cagtgtttcc
     2701 agtatgactg agcttcgtgc gaatgcacgt aaagaaggcg tttatttgcg cgttctgaaa
     2761 aatactttgg ctcgtcgtgc agtgcaaggt acttcatttg cagaattggc cgatcaaatg
     2821 gttggtccgt tggtttacgc tgcttctgaa gatgctgttg ctgctgctaa agtgttgcac
     2881 caattcgcga aaaaagatga caaaattgtc gttaaagccg gttcttacaa tggcgaagta
     2941 atgaatgctg ctcaggttgc tgagttggct tctattccga gccgcgaaga gctgttgtcc
     3001 aaactgttgt tcgttatgca agctcctgta tcgggctttg cgcgcggttt ggctgctttg
     3061 gcagagaaaa aagccggcga agaagccgct taatcgattt tgtttctgtt aatcaattat
     3121 tttttaatac aatatttgga gtaaaatagc atggctatta ctaaagaaga cattttggaa
     3181 gcagttggtt ctttgaccgt aatggaattg aacgacttgg ttaaagcttt tgaagaaaaa
     3241 ttcggtgttt ctgctgctgc tgttgcagtt gcaggtcctg ctggtgccgg tgctgccgat
     3301 gctgaagaaa aaaccgaatt tgatgtcgtt ttggcttctg ccggcgatca aaaagtcggc
     3361 gtgattaaag ttgtccgtgc aattaccggt ttgggtctga aagaagctaa agacatcgtt
     3421 gacggcgcac ctaaaaccat taaagagggt gtttctaaag ctgaagccga agacatccaa
     3481 aaacaactgg aagaagcagg cgctaaagtc gaaatcaaat aatttgatgc ttcttatgaa
     3541 ggctggcagt tttctgccag ccttattttg cttcttaaaa taaacatcaa gtattgttta
     3601 catttatttg catagttttt atcaagtcat tgcaaataaa tgtaaatatc agattgatgc
     3661 gtaccgttgt ttcagacggc ctattattga aaattacttt tcggagtgtg tatgaactat
     3721 tcgtttaccg agaaaaaacg tatccgtaag agttttgcaa agcgggaaaa tgttttggaa
     3781 gttcctttct tgctagcaac ccaaattgat tcttatgcga agtttttgca gctggaaaat
     3841 gcttttgaca aacgtaccga tgacggtctg caggcggcat ttaattctat tttcccgatt
     3901 gtgagccata acggttatgc gcgattggag tttgtgcatt acacattggg cgagcctttg
     3961 ttcgatattc ccgaatgtca gttgcgcgga atcacttatg cagccccctt gcgcgcgcgt
     4021 atccgtttgg tgattttgga taaggaagca tctaaaccga cggtaaaaga agttcgtgaa
     4081 aacgaagtgt atatgggcga aattccgttg atgaccccga gcggttcttt tgtgattaac
     4141 ggcacagagc gtgtgattgt ctcccagttg caccgttcgc ccggcgtatt cttcgagcat
     4201 gacaaaggta agacgcactc ttccggcaaa ttgttattct ccgcccgcat cattccctac
     4261 cgtggttcat ggttggattt tgaatttgat ccgaaagatt tgctgtattt ccgtatcgac
     4321 cgccgccgta aaatgccggt aacgattttg ttgaaggctt taggctacaa caatgagcaa
     4381 atcttggata ttttctacga caaagaaacg ttctatttgt cttcaaacgg tgttcaaacc
     4441 gatttggttg cagaccgtct gaaaggcgaa actgccaagg tcgatatctt ggataaagaa
     4501 ggcaatgtat tggttgccaa aggtaagcgc attactgcga aaaatatccg tgatattacc
     4561 aatgcaggcc tgacccgttt ggatgtagaa ccggaaagcc tgctgggcaa agcattggct
     4621 gccgatctga ttgattcgga aaccggcgag gtattggctt ctgccaatga tgaaattaca
     4681 gaagagttgt tggccaaatt tgatatcaac ggcgtaaaag aaattacgac cctttatatc
     4741 aatgagctgg atcagggtgc ttatatctcc aataccttgc gtacggatga gactgccggc
     4801 cggcaggcgg ctcgtgttgc gatttaccgt atgatgcgtc cgggcgaacc gcccaccgaa
     4861 gaggcggtcg agcaattgtt taaccgcttg ttcttcagtg aagacagcta cgatctgtcc
     4921 cgcgtaggcc gtatgaaatt taatacgcgc acatacgaac aaaaactgtc cgaagcccaa
     4981 caaaactctt ggtacggccg cctgctgaac gaaacgtttg ccggtgctgc cgacaaaggc
     5041 ggttatgtcc tgagcgtcga agatattgtc gcctcgattg cgactttggt cgagttgcgt
     5101 aacggccatg gcgaagtgga cgatatcgat cacttgggca accgccgagt acgttcggta
     5161 ggcgagctga ctgaaaacca attccgtagc ggtttggccc gtgtggaacg tgccgtaaaa
     5221 gaacgtttga atcaggcgga atcagaaaac ttgatgccgc acgatttgat taatgcaaaa
     5281 cctgtttctg ccgctattaa agaattcttc ggctccagcc aattgagtca gtttatggat
     5341 cagaccaacc ccttgtctga agtaacccat aaacgccgtg tatctgcatt gggtccgggc
     5401 ggtttgaccc gcgaacgtgc aggatttgag gtgcgggacg tgcatccgac ccactacggt
     5461 cgcgtatgtc cgattgaaac gcctgaaggt ccgaacatcg gtttgatcaa ctcattgtcc
     5521 gtttatgcgc gcaccaatga ttacggtttc ttggaaacgc cttaccgccg cgttatcgac
     5581 ggcaaagtaa ccgaggaaat cgattacttg tctgccatcg aagaaggccg ctatgtgatt
     5641 gcacaggcga atgccgattt ggattcagat ggcaatctga ttggcgattt ggttacctgt
     5701 cgtgaaaaag gcgaaaccat tatggcaacg cccgaccgcg tccaatatat ggacgtggca
     5761 actggtcaag tggtatccgt tgcagcatcc ctgattccat tcttggaaca tgatgacgcg
     5821 aaccgcgcat tgatgggtgc caacatgcaa cgtcaggcag tgccttgctt gcgtcctgaa
     5881 aaaccgatgg tcggtaccgg tatcgagcgt tccgttgccg ttgactctgc tactgcaatc
     5941 gttgcccgcc gaggcggcgt ggtcgagtat gtcgatgcca accgcgttgt gatccgtgtc
     6001 catgacgacg aagcgactgc cggtgaagtg ggtgtcgata tttacaactt ggttaaattc
     6061 acccgttcca accagtctac caatatcaat cagcgtcctg ccgtcaaagc cggcgatgtt
     6121 ttgcaacgcg gcgatttggt ggccgacggc gcgtccaccg attttggcga attggctttg
     6181 ggtcaaaata tgaccatcgc cttcatgccg tggaacggtt acaactacga agactcgatt
     6241 ctgatttccg aaaaagtggc tgcggacgac cgctatactt cgattcacat tgaggaattg
     6301 aatgtcgttg cccgcgatac taagctgggt gcggaagaca ttacccgcga tattccgaac
     6361 ttgtccgagc gtatgcaaaa ccgtttggac gaatccggta tcgtttacat cggtgcggaa
     6421 gtagaagccg gcgatgtgtt ggtaggcaag gtaacgccta aaggcgaaac ccaactgacg
     6481 ccggaagaaa aactgctgcg cgccatcttc ggtgaaaaag catctgacgt aaaagatact
     6541 tcattgcgta tgcctaccgg catgagcggt accgttatcg acgttcaagt cttcactcgt
     6601 gaaggtattc aacgcgacaa acgtgctcaa tccattatcg attccgaatt gaaacgctac
     6661 cgtttggatt tgaacgacca attgcgtatt ttcgacaacg acgcattcga ccgtatcgag
     6721 cgtatgattg tcggtcagaa agccaacggt ggtccgatga agctggccaa aggcagcgaa
     6781 atcacgaccg aatatctggc gggtctgccg agcaggcacg attggttcga tatccgtctg
     6841 accgatgaag atttggccaa gcagttggaa ctgattaaag tgagcctgca acaaaaacgc
     6901 gaagaagcgg acgagttata cgaaatcaag aagaaaaaac tgacccaagg cgacgaattg
     6961 caacccggcg tacaaaaaat ggtgaaagtt tttatcgcca tcaaacgccg tctgcaagcc
     7021 ggcgacaaaa tggcgggccg ccacggtaac aaaggcgtgg tatcgcgcat tctgccagtg
     7081 gaagacatgc cttacatggc ggacggccgt ccggtagaca tcgtactgaa cccattgggc
     7141 gtaccttccc gtatgaacat cggtcagatt ttggaagttc acttgggttg ggcagcaaaa
     7201 ggtatcggcg agcgtatcga ccgtatgctg aaagagcaac gcaaagcagg cgagttgcgc
     7261 gagttcttga acagactcta caacggcagc ggtaagaaag aagatttgga tgccctgact
     7321 gatgaagaaa tcatcgaact ggcctccaac ctgcgcaaag gtgcatcttt cgcctctcct
     7381 gtattcgacg gtgcgaaaga gtctgaaatc cgcgaaatgc tgaacttggc ttatccgagc
     7441 gacgatcctg aggttgaaaa actgggcttc aacgacagta aaacccaaat cacgctgtat
     7501 gacggccgtt caggcgaagc atttgaccgc aaggttacag taggtgtgat gcactatctg
     7561 aaactgcacc acttggttga cgaaaaaatg cacgcgcgtt ctaccggtcc gtacagtctg
     7621 gttacccagc agcctttggg cggtaaagcc cagttcggcg gccaacgttt cggcgagatg
     7681 gaggtttggg cattggaagc atacggcgcg gcatacacgc tgcaagagat gctgactgtg
     7741 aagtctgacg acgtgaacgg ccgtaccaaa atgtacgaaa acatcgtcaa aggcgaacac
     7801 aaaatcgatg ccggtatgcc cgagtccttc aacgtattgg tcaaagagat tcgctcactg
     7861 ggcttggata tcgatttgga acgttactaa acaaaagttt tcagacggcc tttcagggtc
     7921 gtctgaaaaa gtggtttcag aataagaatg aagcaatcgg catttaggcc gtctgaaatc
     7981 aaaagtaccg tttcccaata tcgaaaatcc gccatgcggt aaaaatactt ccttcaagga
     8041 gcaaaaatga atttgttgaa cttatttaat ccgttgcaaa ctgccggcat ggaagaagag
     8101 tttgatgcca ttaaaatcgg tattgcctct cccgaaacca tccgctcatg gtcttatggc
     8161 gaagtcaaaa aacctgaaac catcaactac cgtacgttca aacctgagcg tgacggtttg
     8221 ttctgtgcca aaatctttgg cccggtcaaa gactacgaat gcttgtgcgg aaaatacaaa
     8281 cgcttgaaat ttaaaggcgt aacgtgtgaa aaatgcggcg tggaagtaac cctgtccaaa
     8341 gtgcgccgcg aacgcatggg tcatatcgaa ttggctgcgc ccgtcgcaca tatttggttc
     8401 ttaaaatccc tgccttcccg cttgggtatg gtgttagaca tgactttgcg cgacatcgag
     8461 cgcgtattgt actttgaagc atttgtggta accgatcccg gtatgactcc gctgcaacgc
     8521 cgccaattgc tgactgaaga cgattactac aacaagctgg acgaatacgg cgacgatttc
     8581 gatgccaaaa tgggtgcgga aggtatccgc gaattgctgc gtaccctgaa tgtagcgggc
     8641 gaaatcgaaa tcctgcgcca agagttggaa tcgaccggtt ccgacaccaa aatcaaaaaa
     8701 atcgccaaac gcttgaaagt attggaagcc ttccatcgtt ccggtatgaa actggaatgg
     8761 atgattatgg atgtgctgcc ggtattgccg cctgatttgc gtccgttggt tccattggat
     8821 ggtggtcgtt ttgccacttc cgatttgaac gatttgtacc gccgcgttat taaccgtaac
     8881 aaccgtctga aacgtctgtt ggaactgcat gcgcctgaca tcatcgtccg caacgaaaaa
     8941 cgtatgttgc aagaagcagt tgactcgctg ttggataacg gccgtcgcgg taaagccatg
     9001 accggcgcca acaaacgccc gctgaaatca ttggcagaca tgattaaagg taaaggcggt
     9061 cgcttccgtc aaaacctgtt gggcaaacgt gtggactact ccggccgttc cgtgattacc
     9121 gtaggcccgt acctgcgtct gcaccaatgc ggtttgccga aaaaaatggc tttggaactg
     9181 ttcaaaccgt tcattttcca caaattggaa aaacaaggtt tggcctctac cgttaaagca
     9241 gcgaaaaaat tggtagagca agaagtaccg gaagtatggg acatcttgga agaagtcatc
     9301 cgcgaacatc cgattatgct gaaccgtgcg ccgaccctgc accgtttggg tattcaagcg
     9361 ttcgaaccta tcttgattga aggtaaagcg attcagttgc acccattggt gtgtgctgcg
     9421 ttcaacgccg actttgacgg cgaccaaatg gcggtacacg ttccattgag cttggaagca
     9481 caaatggaag cacgcacgct gatgctggct tcaaacaacg tattgtctcc ggccaacggc
     9541 gaaccgatta tcgtaccttc ccaagacatc gtattgggcc tgtactatat gactcgcgat
     9601 cgtatcaatg ccaaaggcga aggcagcctg tttgccgatg tgaaagaagt gcatcgcgca
     9661 taccatacca aacaggtcga gctgggtacg aaaatcaccg tacgtctgcg cgaatgggtg
     9721 aaaaacgaag caggtgagtt tgagcctgtc gttaaccgtt acgaaacaac cgtcggccgt
     9781 gcattgttga gcgaaatcct gccgaaaggc ctgccgtttg aatatgtcaa caaagcgttg
     9841 aagaaaaaag aaatttctaa actgattaac gcatcgttcc gcctgtgcgg cttgcgcgat
     9901 acggttatct ttgctgacca cctgatgtac accggtttcg gatttgcggc aaaaggcggt
     9961 atttccattg ccgttgacga tatggaaatt ccaaaagaaa aagcggcctt gctggctgaa
    10021 gccaatgccg aggttaaaga aatcgaagac caataccgtc aaggtttggt taccaacggc
    10081 gaacgctaca acaaggtggt cgatatttgg ggtcgtgccg gcgataagat tgctaaagcg
    10141 atgatggaca acttgtccaa acaaaaagtt atcgaccgtg ccggcaacga agtcgatcaa
    10201 gagtcattca actccattta tatgatggcg gactccggtg cccgtggttc tgcagctcag
    10261 attaaacagt tgtccggtat gcgtggcttg atggcaaaac ctgacggctc gattattgaa
    10321 acgccgatta cctcaaactt ccgtgaaggt ctgaccgtat tgcaatactt tattgcgacc
    10381 cacggtgcgc gtaagggttt ggcggatacc gcattgaaaa ccgcgaactc cggttacctg
    10441 actcgtcgtc tggtagacgt aactcaagat ttggtcgttg ttgaagacga ttgcggtact
    10501 tcagacggct ttgtcatgaa ggcagtggta caaggcggtg atgtgattga agcattgcgc
    10561 gatcgtattt tgggtcgtgt taccgcgtct gacgttgtcg atccgtcaag tggcgaaacc
    10621 ttggttgaag ccggtacgtt gctgactgaa aaactggtgg atatgatcga ccaatccggt
    10681 gtcgatgaag tcaaagtccg tacgccgatt acttgtaaaa cccgtcacgg cctgtgtgca
    10741 cactgttacg gtcgtgactt ggcacgcggc aaactggtta acgccggtga ggcagtcggt
    10801 gtgattgctg cacaatccat tggcgaaccg ggtacccagt tgaccatgcg tacgttccac
    10861 atcggtggtg cggcatcccg tgcggcagca gccagccaag tggaagccaa atccaacggt
    10921 acggcacgat tcagcagcca gatgcgctac gttgccaaca acaaaggcga gttggttgtc
    10981 atcggccgtt cttgtgaagt cgtgattcac gacgatatcg gccgtgaacg cgaacgccac
    11041 aaagtacctt acggtgccat cctgctggta caagacggta tggccattaa agccggtcaa
    11101 accttggcaa cctgggatcc gcatacccgt ccgatgatta ccgaacacgc aggtatggtg
    11161 aaattcgaaa acgtggaaga gggcgttacc gttgccaaac aaaccgatga tgtaaccggt
    11221 ttgtccactt tggtggtgat tgacggtaaa cgtcgttcct ctagtgcttc caaactgctg
    11281 cgtccgactg tgaaactctt ggacgaaaac ggcgtggaaa tctgtattcc cggtacttct
    11341 actccggtat ccatggcatt ccccgttggt gcggtgatta ccgtacgcga aggtcaggaa
    11401 atcggtaaag gcgacgtatt ggcgcgtatt ccgcaagcct cttccaaaac ccgcgacatt
    11461 accggcggcc tgccgcgcgt tgccgaattg tttgaagcac gcgtgccgaa agatgccggt
    11521 atgttggcgg aaattaccgg taccgtttcc ttcggcaaag agaccaaagg caagcaacgt
    11581 ctgattgtta ctgacgtgga cggtgtagca tacgagacct tgatttccaa agagaaacaa
    11641 attctggtac acgacggtca agtggtaaac cgcggtgaaa ccatcgtgga cggcgcggtc
    11701 gatccgcacg atattctgcg tttgcaaggt atcgaagcac tggcacgcta cattgtccaa
    11761 gaggtgcaag aggtttaccg tctgcaaggt gtgaagattt ctgataaaca catcgaagtc
    11821 atcatccgtc aaatgttgcg ccgtgtgaac attgcggatg ccggcgaaac cgggttcatt
    11881 accggagagc aggtcgaacg cggcgatgtg atggcggcca atgaaaaagc tttggaagaa
    11941 ggcaaagaac cggcgcgtta cgaaaacgta ttgctgggta ttaccaaagc ttccctgtcc
    12001 accgacagct tcatttctgc cgcatcgttc caagaaacga cccgcgttct gaccgaagcc
    12061 gcgattatgg gcaaacaaga cgagttgcgt ggtttgaaag aaaacgtcat cgtcggtcgc
    12121 ttgattcctg ccggtaccgg tttgacttac caccgcagcc gtcatcaaca atggcaagag
    12181 gtggaacagg agactgccga aacccaagta acggatgaat aatctttggt gcatccattc
    12241 aataaaaaac cgcaagcctt gagcttgcgg tttttctttg tccgattaag gcaaaaacaa
    12301 gcgttttcgt cattttgagg cgtgtggatt attccttagg tattttcggg ccggagacca
    12361 acgaggtggc gggtgtcgtc ggtacgtccg gagaccaaaa taactttgcc agggatgttg
    12421 gtttcggcgg tcaaaaaaag tagcgtctta atgttttcca tttaaacaaa tgtcgtctga
    12481 aacttcagac ggcatttcct ttaagaaata aatatgaaac ccagaaatct cttttttgca
    12541 ggctgcctgc tgacttcggc gacgtttgcc gaggatatcg gcgtacctgt cgaactgatt
    12601 aacgtcggta atcggattgc gatgccgtct gaaggggaaa gcctcgccct cctgccgttt
    12661 gccgaggatg taccgccggt tcgcgatgca atgccgtctg aagttcctaa aagcgcggca
    12721 ggcggcgatg ttcggggtga ccggatgaga atgccgatta acatcggatg agcgcggctt
    12781 tatggcataa aaaactgtcg tggaaaggat ttacacccca aataaatttc cgttacaaca
    12841 agatcaacag caatatgccc gccttttatt cgcgcagcgg caaggaacgg tttgtcagta
    12901 tagaaaaaac gtattgacag tattttcttc agtcgtccga ctgattgtga gggatgtcgg
    12961 taaatattta tcggcaaaca agaaaatcat ctttcttctt gtcgttatgc ttgactgtct
    13021 gcttgcaata aaaatataat tccactcttg ccgacatggt gtcggcaagt atttaactca
    13081 acaggacgag aaaatatgcc aactatcaac caattagtac gcaaaggccg tcaaaagccc
    13141 gtgtacgtaa acaaagtgcc cgcactggaa gcttgcccgc aaaaacgtgg cgtgtgcacc
    13201 cgtgtataca caactacccc taaaaaacct aactctgcat tgcgtaaagt atgtaaagtc
    13261 cgcctgacca acggttttga agtcatttca tacatcggcg gcgaaggtca caacctgcaa
    13321 gagcacagtg tcgtattgat tcgcggcggt cgtgtaaaag acttgccagg tgtgcgttac
    13381 cacactgtac gcggttcttt ggatactgca ggtgttaaag accgtaaaca agcccgttcc
    13441 aaatacggtg ctaagcgtcc taaataatta ctgggactta aataggcacg tcggccgcct
    13501 aagctgaaca acggccgagt aagtgaatac tcaattgggt attcatggga atagacccga
    13561 ctgaatagat taaaggaaat taaaatgcca agacgtagag aagtccccaa gcgcgacgta
    13621 ctgccagatc ctaaattcgg cagcgtcgag ttgaccaaat tcatgaacgt attgatgatt
    13681 gacggtaaaa aatccgttgc cgagcgtatc gtttacggtg cgttggaaca gattgagaaa
    13741 aaaaccggca aagtagcaat cgaagtattt aacgaagcca ttgcaaacgc caaacctatc
    13801 gtggaagtga aaagccgccg tgtaggtggt gcaaactacc aagttcctgt tgaagttcgt
    13861 ccttcacgcc gtttggcttt ggcaatgcgc tgggttcgcg atgcggcccg caaacgtggt
    13921 gagaaatcca tggacctgcg tttggcaggc gaattgattg atgcgtccga aggccgtggc
    13981 ggtgcgttga aaaaacgtga agaagtacac cgtatggctg aagccaacaa agcattctct
    14041 cacttccgtt tctaattttg aaaggctaat aaaatggctc gtaagacccc gatcagcctg
    14101 taccgtaaca tcggtatttc cgcccatatt gacgcgggta aaaccacgac gacagaacgt
    14161 attttgttct ataccggttt gacccacaag ctgggcgaag tgcatgacgg tgcggctact
    14221 accgactaca tggaacaaga gcaagagcgc ggtattacca ttacctccgc tgccgttact
    14281 tcctactggt ccggtatggc gaaacaattc cccgagcacc gcttcaacat catcgacacc
    14341 ccgggacacg ttgactttac cgtagaggta gagcgttcta tgcgtgtatt ggacggcgcg
    14401 gtaatggttt actgcgcggt gggcggtgtt caaccccaat ctgaaaccgt atggcggcaa
    14461 gccaacaaat accaagtgcc gcgcttggcg tttgtcaata aaatggaccg tcagggtgcc
    14521 aacttcttcc gtgttgtcga gcaaatgaaa acccgtttgc gcgcaaaccc tgtacctatc
    14581 gtcattccgg ttggtgcgga agacaacttc agcggtgtgg ttgatttgtt gaaaatgaaa
    14641 tccatcattt ggaatgaagt cgataaaggt acaaccttta cctatggcga tattcctgcc
    14701 gaattggtcg aaactgccga agaatggcgt caaaatatga ttgaagccgc agccgaagcc
    14761 agcgaagaac tgatggacaa atacttaggc ggcgacgagc tgaccgaaga agaaatcgta
    14821 ggcgcgttgc gtcaacgtac tttggcaggc gaaattcagc ctatgctgtg tggttctgca
    14881 tttaaaaaca aaggtgttca acgtatgttg gacgcagttg tagaattgct gccagctcct
    14941 accgatattc ctccggttca aggtgtcaac ccgaataccg aggaagccga cagccgtcaa
    15001 gccagcgatg aagagaaatt ctctgcattg gcgttcaaaa tgttgaacga caaatacgtc
    15061 ggtcagctga cctttatccg cgtttactca ggcgtagtaa aatccggcga taccgtattg
    15121 aactccgtaa aaggcactcg cgaacgtatc ggtcgtttgg tacaaatgac tgccgcagac
    15181 cgtactgaaa tcgaagaagt acgcgccggc gacatcgcag ccgctattgg tctgaaagac
    15241 gttactaccg gtgaaacctt gtgtgcggaa agcgcgccga ttatcttgga acgtatggaa
    15301 ttccccgagc cggtaatcca tattgccgtt gagccgaaaa ccaaagccga ccaagagaaa
    15361 atgggtatcg ccctgaaccg cttggctaaa gaagaccctt ctttccgtgt ccgtacagac
    15421 gaagaatccg gtcaaaccat tatttccggt atgggtgagc tgcacttgga aattattgtt
    15481 gaccgtatga aacgcgaatt cggtgtggaa gcaaatatcg gtgcgcctca agtggcttac
    15541 cgtgaaacta tccgcaaagc cgttaaagcc gaatacaaac atgcaaaaca atccggtggt
    15601 aaaggtcaat acggtcacgt tgtgattgaa atggaaccta tggaaccggg tggtgaaggt
    15661 tacgagttta tcgatgaaat taaaggtggt gtgattcctc gcgaatttat tccgtctgtc
    15721 gataaaggta tccgcgatac gttgcctaac ggtatcgttg ccggctatcc tgtagttgac
    15781 gtacgtatcc gtctggtatt cggttcttac catgatgtcg actcttccca attggcattt
    15841 gaattggctg cttctcaagc gtttaaagaa ggtatgcgtc aagcatctcc tgccctgctt
    15901 gagccaatca tggcagttga agtggaaacc ccggaagaat acatgggcga cgtaatgggc
    15961 gacttgaacc gccgtcgcgg tgttgtattg ggtatggatg atgacggtat cggcggtaaa
    16021 aaagtccgtg ccgaagtacc tttggcagaa atgttcggtt actcgaccga cctgcgttct
    16081 gcaacccaag gccgcgctac ttactctatg gagttcaaga aatattctga agctcctgcc
    16141 cacatagctg ctgctgtaac tgaagcccgt aaaggctaat cagaaaaggc cgtctgaaac
    16201 tgaaaataaa ttttcagacg gccattgttc tttaatcgat ctttatatgt aaaggaatta
    16261 gctcatggct aaggaaaaat ttgaacgtag caaaccgcac gtaaacgttg gcaccatcgg
    16321 tcacgttgac catggtaaaa ccactctgac tgctgctttg actactattt tgtctaaaaa
    16381 attcggtggc gctgcaaaag cttatgacca aatcgacaac gctcctgaag aaaaagctcg
    16441 tggtattacc attaatacct cacacgtaga atacgaaact gaaacccg
//
LOCUS       DAWIVB010000027        16380 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWIVB010000027 DAWIVB010000000
VERSION     DAWIVB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.027"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(91..465)
                     /locus_tag="WIX60_000625"
     CDS             complement(91..465)
                     /locus_tag="WIX60_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304931.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNSRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(638..994)
                     /locus_tag="WIX60_000626"
     CDS             complement(638..994)
                     /locus_tag="WIX60_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ5304932.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(1137..1481)
                     /locus_tag="WIX60_000627"
     CDS             complement(1137..1481)
                     /locus_tag="WIX60_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304933.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(1640..2041)
                     /locus_tag="WIX60_000628"
     CDS             complement(1640..2041)
                     /locus_tag="WIX60_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304934.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(2045..2785)
                     /locus_tag="WIX60_000629"
                     /pseudo
     CDS             complement(2045..2785)
                     /locus_tag="WIX60_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2855..3172)
                     /locus_tag="WIX60_000630"
     CDS             complement(2855..3172)
                     /locus_tag="WIX60_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304935.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(3169..3771)
                     /locus_tag="WIX60_000631"
                     /pseudo
     CDS             complement(3169..3771)
                     /locus_tag="WIX60_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(4197..4541)
                     /locus_tag="WIX60_000632"
     CDS             complement(4197..4541)
                     /locus_tag="WIX60_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304936.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(4579..4923)
                     /locus_tag="WIX60_000633"
     CDS             complement(4579..4923)
                     /locus_tag="WIX60_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304937.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(4926..5114)
                     /locus_tag="WIX60_000634"
     CDS             complement(4926..5114)
                     /locus_tag="WIX60_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ5304938.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(5184..5528)
                     /locus_tag="WIX60_000635"
                     /pseudo
     CDS             complement(5184..5528)
                     /locus_tag="WIX60_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(5612..5914)
                     /locus_tag="WIX60_000636"
     CDS             complement(5612..5914)
                     /locus_tag="WIX60_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304939.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(5937..6206)
                     /locus_tag="WIX60_000637"
     CDS             complement(5937..6206)
                     /locus_tag="WIX60_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304940.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
     gene            complement(6227..6433)
                     /locus_tag="WIX60_000638"
     CDS             complement(6227..6433)
                     /locus_tag="WIX60_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304941.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(6399..7148)
                     /gene="mafB"
                     /locus_tag="WIX60_000639"
                     /pseudo
     CDS             complement(6399..7148)
                     /gene="mafB"
                     /locus_tag="WIX60_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(7254..7625)
                     /locus_tag="WIX60_000640"
     CDS             complement(7254..7625)
                     /locus_tag="WIX60_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ5304942.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(7622..7873)
                     /locus_tag="WIX60_000641"
     CDS             complement(7622..7873)
                     /locus_tag="WIX60_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304943.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(8005..8211)
                     /locus_tag="WIX60_000642"
     CDS             complement(8005..8211)
                     /locus_tag="WIX60_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304944.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(8221..8889)
                     /gene="mafB"
                     /locus_tag="WIX60_000643"
                     /pseudo
     CDS             complement(8221..8889)
                     /gene="mafB"
                     /locus_tag="WIX60_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(9013..9369)
                     /locus_tag="WIX60_000644"
     CDS             complement(9013..9369)
                     /locus_tag="WIX60_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ5304945.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(9366..10817)
                     /gene="mafB"
                     /locus_tag="WIX60_000645"
     CDS             complement(9366..10817)
                     /gene="mafB"
                     /locus_tag="WIX60_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ5304946.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKKRVYAVQTFDATAVSPVLPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDVRGIVQGAVNPFLMGFQGVGIGAITDSAVSPVTDTAAQQTLQGINDLGKLS
                     PEAQLAAASLLQDSAFAVKDGINSAKQWADAHPNITATAQTALSAAEAAGTVWRGKKV
                     ELNPTKWDWVKNTGYKKPAARHMQTLDGEMAGGNKPIKSLPNSAAEKRKQNFEKFNSN
                     WSSASFDSVHKTLTPNAPGILSPDKVKTRYTSLDGKITIIKDNENNYFRIHDNSRKQY
                     LDSNGNAVKTGNLQGKQAKDYLQQQTHIRNLDK"
     gene            complement(10857..11820)
                     /gene="mafA"
                     /locus_tag="WIX60_000646"
                     /pseudo
     CDS             complement(10857..11820)
                     /gene="mafA"
                     /locus_tag="WIX60_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
     gene            complement(12004..12723)
                     /locus_tag="WIX60_000647"
     CDS             complement(12004..12723)
                     /locus_tag="WIX60_000647"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ5304947.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVITGEDEGTLVHC"
     gene            complement(12940..13794)
                     /locus_tag="WIX60_000648"
     CDS             complement(12940..13794)
                     /locus_tag="WIX60_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ5304948.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFSKENGTEVISFVRYKVGDGIEKKAVDYAA
                     EVAAAAKV"
     gene            complement(13923..14651)
                     /gene="rpsB"
                     /locus_tag="WIX60_000649"
     CDS             complement(13923..14651)
                     /gene="rpsB"
                     /locus_tag="WIX60_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ5304949.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(14824..15057)
                     /locus_tag="WIX60_000650"
     CDS             complement(14824..15057)
                     /locus_tag="WIX60_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304950.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            15135..15716
                     /locus_tag="WIX60_000651"
     CDS             15135..15716
                     /locus_tag="WIX60_000651"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ5304951.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAELYLPYIEPRSRRMWFTPYPADGVKQERKRGR
                     AKLHVPQFAGRKKRVHDLNLLLVPVVGMDRLGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPVEAHDRSLDGFVSEAGILCF"
     gene            15777..16367
                     /locus_tag="WIX60_000652"
     CDS             15777..16367
                     /locus_tag="WIX60_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ5304952.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDSAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         4264 a         3411 c         3334 g         5371 t
ORIGIN      
        1 ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc gagtttatcc tagttgtcca
       61 gggcagcctc atctctttat agatagctgc ttaccaagta tccttaattg ctaattcaag
      121 ccaatcttca aaactattaa aaaattcatg gttttctata ttatctaaat taatgacaat
      181 aaccctagaa tttcctgtta catcattacc atcaaaataa gcttcccatt ctccattact
      241 agcgaatggg ataagattta aatcaggcag gatagaattt ctaaatatat aagctacttc
      301 tgccccttct gttccataat tctcaaacca ccagtgaaaa ttatgtggct gaatataagg
      361 tattaaagat gtatcgttag agatacgaac atacttttca ggataacaaa acctttcagg
      421 aagcatatta ctaggatata aattaaattc attttgaata atcatttcgt taccttagga
      481 tcataaatac taccattagg aaactgggta gaccaatttg gtggtgctag ttcattttca
      541 atttgttttt taattctaaa ttgaatagct tctccccatg tttgcggcat ctaatgctgc
      601 ttgctctgca tcaatattac taaaaattgg attagtctca aaaatgaaaa attactgttt
      661 tttcattttt tatagcatat tcaagtacat gaataatatt tttcactaag ggctctgtag
      721 aaaaatctga attttgtaat ttttctttta cagccaaaat actttcctta tctattagcc
      781 aatccccctc acttggtcca aataaccaat tatcggtaat ttcagaaaca agtctccata
      841 atccacgatt ttgatattca ataatttgat tttccgtaat ttctacaact gtttcagatt
      901 gaatgttttt ataagcccaa tcaatcattt cagtatattc actaattgaa acattgtctt
      961 ttaaacagaa aattaacagg ttgtgttcat tcatttgtct aagttcctga tatgagtttg
     1021 ttgttgtaaa taatcttttg cttgcttacc ttgtaaatta aaggattaac cgcaccttgg
     1081 atgatgccgc gaatatcatc catacggtta ccccaccaat ttttattggt gtatcgttaa
     1141 tcatttattt caaaaaagtt atttattatc atattatcag ggagcaggtc gttaaaaaaa
     1201 accccctcaa attccattaa attagtattt atccagcaca taaacgaacc aaagcgttct
     1261 ttccctgagt gtaaatattt aaaattaaac tcaaaataag tttttttgct tagaaaattt
     1321 aatggttttt ctatagtgaa aaaaaagccc ttactcgttt tcgttcgaga tactatttta
     1381 aataaatttt tattttcaat aaaataagaa tattcattta ctaagttgtt atgataaaaa
     1441 agtaatttta ttaaaatgtt ttcataattt tctaggttca tttctagctc aatttaaaat
     1501 gatttcattg ttgccgtacc aatgcgaaat ttaccatcaa caaaatttcc cttcactgat
     1561 acaggtacac catcaacttg ggtagtttta ttttcaggct gcctgaaaga aagataggca
     1621 acttattact catcttacgc tagccctcaa taattgaata cgttgcacta gaaccaatta
     1681 tttctatgaa tgtttgctca aacataaaaa tatagtattt aaagaattct ctattcaaag
     1741 gaaaataact ttctttcaat atccattccg cataacactc atcaagaagt tcaaaaaaag
     1801 attgaccacc attttcatga agcaaagctc tatctgcatc ttccgaaatt aaatccataa
     1861 aagtatctat aaaatacgat tcatcaacaa ccctaaaatg aataacatct tcaataaaaa
     1921 tttttatggt tttatccgta tttctataac caactgctat aacttctact ttatcagagt
     1981 ttattgctgc attatcaaaa tagaaatagc cagttgggaa atctaagctt tttaatttca
     2041 taatttatcg tgtatttcca tatctgattt ttatttttcg gttattacta attgttattt
     2101 caagtgttgg ttctccacca ctactatcat tacgagcatt aaccgcacgc ccatcaggta
     2161 aattgcccgt tatcgttcca ttaggacgtg tttgtacatt tcgaacattc agactattaa
     2221 aatcatttag tgcgtcattg taatccccca ctttatcata ctgaacagcg ggaccttttg
     2281 ttttactcct ttgagtagta ttgctcaaaa tgtctttgat ttgtaaatca ctgcgcgcct
     2341 taccggcttc tatgcgagct gccacactac tcgggctata ggggtcgcta tcaatcgcag
     2401 gattaccacc gctggttttt tgtccgctag cctgtttccc aacctgctgt actgtatcgc
     2461 caatttcatc taccgtacct aacgccttag tctgcatatg gcgaacagca ggttttttat
     2521 agccggtatt tttaacccaa tcccatttgg tcgggttaag ttctactttt ttacctctcc
     2581 aaaccgtacc tgcggcctct gctacggcaa gggcagtttg ggctgttgct gttatattcg
     2641 gatgggcatc agcccattgt ctggcggaat tgatgccgtc ttttaccgca aaagcactgt
     2701 cctgtaatat gctcgcggca gcaagttgtg cttccgggct taattttcct gaattattaa
     2761 taccttgtag agtctgctgc gcagagatgt agcaactgta tttttacccc gtcgggtaaa
     2821 aatacagttg ctacggctgc tgggaaagca gttactattt tcccagtggc tcaaaattaa
     2881 ttgtttcagc agatattttt ataataatcc ctacatcaat aataatttca ctataaaatt
     2941 ggtatttata tagctctatt tcgggtataa acaagggaaa caagctatct tccttatcct
     3001 cataatctac gaaattattt gtatctaatt taaaattcaa aatattactg catttgatat
     3061 atccgaaata attaccttca attgtatctg taaaagataa tgtaagggta tttccattat
     3121 cggaaaagat tatttcataa aggcttacat tgtgaaaaat attttttttc atttgacaaa
     3181 atatccagaa tgatgatgat tatttggcaa tggattgcct tcataatctt gtcgttgcgg
     3241 atattttccc tgtacaccat cggttcgcgg cactctttgg gttcttataa cctctccatt
     3301 tggacgaata tctattttca cgccatcagg acctttccat gtttcatatt gagccgcatc
     3361 tgaaccagta cgtctaaaac cttgttcttg caatgcttga cgtaattcat ctcgcgtagt
     3421 tggtctgata gatttaacaa ctggcttgtt tcctcctgcc atctcaccgt ccaacgtctg
     3481 catagggcgg gcagcaggtg ttttatagcc gttattttta acccaatcct atttggtcgg
     3541 gttaagttct acttttttac ctctccaaac cgtacctgcg gcctctgcta cggcaagggc
     3601 agtttgggct gttgcagtta tattcggatg ggcatcagcc cattgtctgg cggaattgat
     3661 accgtctttt accgcaaaag tactgtccta taatgcgctt gcggcagcaa gttgtgcttt
     3721 cggacttaaa tttcctaaat cattaatacc ttgtaaagtt tgctgcgcag agatgtaggt
     3781 cggatatttg tatccgacaa aaatatttga cgcatctatc gtttccgaaa tgccgctgtt
     3841 ggaaaatgtc ggattcgaga atccgaccta cggctatttc gtttaagtca taaatagcca
     3901 ttcatgttcc ttctgaattt gcttttgcta tccttagaac caatcatata aacattatca
     3961 tcattaatta atgttctaag tttatgaagc ggtcaaatct gtcaaactac cagagatgaa
     4021 gcatatcctc ctggaagtaa ttctgttcgt gctgtttcca tatcttcttt gccatcatca
     4081 gttacttctc tgtctaaata aaatcttacg ataagttttt gtctaatttc atcaaattct
     4141 gccgtaatta atcttatatt gatatgaata cacggttcat acctgctatt agccagtcag
     4201 gtgtagttgg atggggattc atttttaata atatcttcaa gcaagtgaaa tgtaagaaaa
     4261 gcgtagatac ttgaaggccg gctatctgat tggcaaaaag aaatttcgga gataaagaat
     4321 ttccgtttat atttttcata aattaaatca acaccttgtt ttacataaaa actaacttcc
     4381 tcaggaagga tatttataac attctctcct tttactttta tagttaatgg tttgattttt
     4441 atgttttcat gaatatctga taggattatt tctaaaaaat tatttttatt tccagtcatt
     4501 ctggctattt tatgtatatt tccattgtgt tcgattatca ttttattttt tccaaggatt
     4561 tccttctata ttgatagttt atctaaagat ttcatttaaa atatcctgac taatttcttg
     4621 tttatataat tctacaattt tctgtttctg tgtatttgta taaaattttt cgttggtatc
     4681 tgcttgatat tttaattgtc tcaatatttc catgatttca tattctgatt tttcatcata
     4741 ttcttcaatc atgtaatcaa caaattgttt tagaatatca aaggcttcgt aactttccaa
     4801 aattttaata tgtttatcgg caaaattgga catataccat tcgtcagtga ttggattagt
     4861 atttaaatca tctaaaaaaa catttaaatc atctaaaaaa acatttacat catctaaaaa
     4921 catgactaat ccttaaactt aaaatctgtt ttctcaccgg ttcttgagtt ttcaatgtaa
     4981 tgtatttcaa ttttgtttac gttttgaaca cgcttaaccc aaccgtcttt tgctaaccaa
     5041 ccatttttag tatcggacat aggaggtatt ctcgcaggag ttttgccctg tgggtttgcc
     5101 ttaacttctt ccattgctaa ttgttccttt aaattattag gtattgtacg accggttgat
     5161 ttttcaacag attgtgtccc tatttttaat gatttgtttc ccccagccat ttccccgtct
     5221 acagtctgca tagggcgggc agcaggtttt ttatagccgg tatttttaac ccaatcccat
     5281 ttagtcgggt taagttctac ttttttaccg cgccaaaccg tacctgcggc ctctgctacg
     5341 gaaagggcag tttgggctgt tgcagttata ttcggatggg catcagccca ttgtctggcg
     5401 gaattaatgc cgttttttac cgcaaaagca ctgtcctgta ataggctcgc ggcagcaagt
     5461 tgtgcttccg gacttaaatt tcctaaatca ttaatacctt gtagagtctg ctgagcggct
     5521 gtatctgagc cggtcagtgt aggttggatt aaaacccagc attttcaaca tcttgatgtt
     5581 ttactataaa tctattcttg tgcttccttt gttagataaa tttcaacaca aaagatacgt
     5641 atgcattctc taattttatt tgagataaat aatgcattgc tttgtcgata ctgttaaata
     5701 aattacttcc ctcgtaatac caattatcat atgtttgata aaaataatta tctttcttga
     5761 tataaatatc tccccccaat acaagtattc tctcattgat aaataaattt aatatatcta
     5821 ttgcaatagg gacgggtaat gcggcttctc taattccaat tcctatcaaa gattcccctt
     5881 gtctcgaata aatttcaaaa atttcattta acatgttaat ctctatctac tatttttcaa
     5941 tcggtataaa ccctattcac agtttcatct tgtatttctt tatgaatact taatatatta
     6001 agatagctta ggaattttat tttctcagat tgaggcaact catcaaaacc aagccaacaa
     6061 taaaaattgt tatatgattt taaaaaatca acaattttta tttttcctaa aaccaaatct
     6121 tcgactaact gggttgcata aataatatca tttatgattt taacaggtaa attatttgat
     6181 aggtaaccat gggattccgt catcattgat ttctctaggt gcttatttat ctgattggta
     6241 agccttttgc gtaagcttct ttcgcctccg caagtgtcat ttcattaggg ccaccatcta
     6301 tattagtttc tcctgtattt tgccactgac aaacgtcaca gatatcatag tccataactt
     6361 cagttccgca aacaggacaa tgtagccata tttctccgtc aatttcccat gtcttttttg
     6421 aattctccat cataatactt ctttcctaag tttggtttaa acattgtttt gattttgaat
     6481 gtttttggat tggccaaaac atagatattg ttattgatat catagcgcac tcgtctatgt
     6541 tccgtctgat aacctaatat cttgtccgat gtctgttttg ataacaaatc agacgccatt
     6601 ttttggtagt cttcaatggt tatatcgcca aattcttttc catgagcttt gaaatgtcca
     6661 tttaaagatt tctcagtagg aaacttggac tttgtccatc tgatgcggtc ttgaattgtt
     6721 cctgtcggag aaatagatgt atcaggttta acatatgagg caggtaagcc gatattgtta
     6781 tcagagtgtg tagggtgttg ctgcgtactt ggttttggtg gcttattccc acctgccatc
     6841 tccccatcta aagtctgcat agggcgggca gcaggtgttt tatagccggt atttttaacc
     6901 caatcccatt tggtcgggtt aaggtttact tttttaccgc cccaaaccgt agttgcggct
     6961 tctgctacgg aaagggcagt ttgggctgtt gcagttatat tcggatgggc atcagcccat
     7021 tgtctggcag agttgatacc gtcttttacc gcaaaagcac tgtcctgtaa taggctcgcg
     7081 gcagcaagtt gtgcttccgg acttaaattt cctaattcat taatatcttg taaagtttgc
     7141 tgcgcagaga tgtaggtcgg atatttgtat ccgacaaaaa tatttgacgc gtctatcgtt
     7201 tccgaaatgc cgctgttgaa aaatgtcgga ttcgagaatc cgacctacgg ttgctaccaa
     7261 ccatcatttt cagcttcttt tagccaagta tcaaaatttt caaaagtctc gtaatatttt
     7321 gtattatcta aattaactac tataatttta ggattacctg atttatctga aatatcaaaa
     7381 caggcagccc actcttggtt tctagcaaaa ggtaataggt ttggaatgcc agttatttca
     7441 aaataaatgt caattacttc tgatatattg ctttctgcat tttcaaacca ccaaggaaaa
     7501 gaatattgtt catcgtagtt aatggcatga gtggattgag ctaattttaa ataactttgc
     7561 ggatatttaa atcctttagg aagcattgct tcaggatacg gaacaaaact attttgtttt
     7621 atcatttcct aagtaccttt acatcataaa tacttccgtt aggaaatctt ttgctccacc
     7681 cttctggaac tcctttttgt gtttcttgct ttctaattcg tagctggata gcttctcccc
     7741 aagtttgagg ctcaatacca tcatgcttaa acctgattct ctcttttgat gataatcggt
     7801 cattaaaatt atgaacattc gcttccactt ttactcctgc ttcacgtgct gccataatac
     7861 gcgtgttatc catactagta ggtgctccat caggcatatt aacaacatca accctgtcgc
     7921 caacccatga tttttttctt tcatttcggc tacaatagaa tcatagttgt aatttgcact
     7981 aggctctgac aaaaatttgg gttgttaagt tgtcattttt ttataaattc cagcaaactg
     8041 ttgttgtaaa ttatctactt catgttttct aacaccagat acattttcaa attctcttga
     8101 atctacacat acgcccgcat atatctcatt caagatagct cgtaaaacaa aaacttcatc
     8161 ttgagttagt gaaatagttg agttttgatt atttatggat agtatattca tggctttttc
     8221 ctataaatca tcataatatt ttttacctga tgttggtcta aatgctgtac ctccatcgtc
     8281 agaattttta tctcggataa ctattgtccc tgttttatca tcccaatacg cagttctacc
     8341 gcgaggtaac tctttcatat ttgttggatg gctaacaata ttttcaatat gccgagcaaa
     8401 atctgctggt gaattgatat ttaaatccgt aaattcttgt tgtcttatga catgcttgtt
     8461 ataagcatgc ccactactaa tttgttctcc aattagttgc gcttgtaaag acggttgtgt
     8521 ggaagcatct gctttgctgt tggacgttat agatttaggc ggtctattcc caccggccat
     8581 ttccccatcc aaagtatgca tggtgcgaac agcaggtgtt ttatagccgg tatttttaac
     8641 ccaatcccat ttggtcgggt taagttctac ttttttaccg ccccaaaccg tagttgcggc
     8701 ctctgctacg gcaagggcag tttgggctgt tgctgttata ttcggatggg catcagccca
     8761 ttgtctggcg gaattgatgc cgtcttttac cgcaaaagca ctgtcctgta ataggctcgc
     8821 ggcagcaagt tgtgcttccg gacttaaatt tcctaattca ttaatatctt gtaaagtttg
     8881 ctgcgcagag atgtaggtcg gatatttgta tccgacaaaa atatttgacg catctatcgt
     8941 ttccgaaatg ccgctgttgg aaaatatcgg attcgagaat ccgacctacg gctgctaaaa
     9001 attagattag tttcaaaaat gaaaaattac tgttttttca ttctttatag catattcaag
     9061 tacatgaata atatttttca ctaagggctc tgtagaaaaa tctgaatttt gtaatttttc
     9121 ttttacagcc aaaatacttt ccttatctat tagccaatcc ccctcacttg gtccaaataa
     9181 ccaattatcg gtaatttcag aaacaagccc ccataatcca cgattttgat attcaataat
     9241 ttgattttcc gtaatttcta caactgtttc agattgaatg ttttcataag cccaatcaac
     9301 catttcagta tattcactaa ttgaaacatt gtcttttaaa cagaaaatta acaggttgtg
     9361 ttcattcatt tgtctaagtt cctgatatga gtttgttgtt gtaaataatc ttttgcttgc
     9421 ttaccttgta aattaccggt tttcacagca ttaccatttg aatcaagata ctgttttcgt
     9481 gaattatcat ggattctaaa atagttgttt tcgttatctt ttataattgt aatttttcca
     9541 tctaaactag tgtatcgagt tttaacttta tcaggactta aaataccagg tgcattggga
     9601 gttagtgttt tgtgcactga atcaaaactt gctgaactcc agttactatt aaacttctca
     9661 aaattttgtt ttcttttttc agcggcactg tttggtaaag atttaatagg tttattccca
     9721 cctgccatct ccccatctaa agtctgcata tggcgggcag caggtttttt ataaccggta
     9781 tttttaaccc aatcccattt agtcgggtta agttctactt ttttacctct ccaaaccgta
     9841 cctgcggcct ctgctgcgga aagggcagtt tgggcagtag ctgttatatt tggatgggca
     9901 tcagcccatt gtttggcaga gttgataccg tcttttaccg caaaagcact gtcctgtaat
     9961 aggctcgcgg cagcaagttg tgcttccgga cttaattttc ctaaatcatt aataccttgt
    10021 agagtctgct gcgcggctgt atctgtgacc gggcttactg cactgtctgt aattgcccca
    10081 atccctactc cttgaaaacc cattaaaaaa ggattaaccg caccttggac gatgccgcga
    10141 acatcatcca tacggttagc ccaccaattt ttattggggt cgctttccca tatcagtttt
    10201 cctgcttcat ccgcacggct gaaaaaacca gaggcggcac cggcattttc ttttagccaa
    10261 cggtctgaaa atggggcttg agggacaata ttagtctttg tttttgttga agttcctttg
    10321 acataatagc tgtatatatc ccttgctcct ccggggggcg gataatcgct gccttgcggc
    10381 ccgtcatatc catcctccgg atggatttcc gaccctgttc gatgaagttg gtaaacagta
    10441 aaaccgccgt ctacaccgcc gctgaaatca gaagtgcttt ttgaatcatg atgatcgaac
    10501 ggactgtgta cttcatgtcc gtgccctgaa aaatgggttt cataaccgat aacaccttca
    10561 aaccctgtcc gttcgtgtgt aataggcagt acaggactga ccgcagttgc atcaaatgtc
    10621 tggacggcgt aaacccgctt tttaacactg ccgcgagcat taccaaacag atggtattta
    10681 ccacccggtt cgtagtgttt tgcctgcata tcatcgcgca aacgggcatc caaaccgttc
    10741 gcatgactaa tggggatttg cagcaacgat attgctgctg caaacagcat catgaatttt
    10801 tgaataggca aattcatttt gttttccttg gtagcaagcg gttatggtag tgtgaatcaa
    10861 ggttgtcctt gtctatgttg tcgcactact tcatcgctgt atccataccc ctcatgactg
    10921 ttatcagcct ctacggatgg ggcggagtta cccgtatgat tgccgtatgg tcggatatcg
    10981 gagaaatcga ccattaatcc ttccgtcggt ttaattcctt tgcttacttt atacggcccc
    11041 atccacaatg cgtaattttc tttataggca gcttcaaacg cattggtttt tggtttgatg
    11101 agcaattttt tattggttct gtctactgcg aaatattcca gttttgtttg ggctttcagt
    11161 gtttcggcat tgtataggtg catttcggtt ctgttgcgta tcgttccgaa tacgtcgatg
    11221 ttaataaaca catctgtatc ggcattggca ggagaaacaa cgtctatgcc gcgcaggaaa
    11281 aatacggtct gtaccaagtg ggaaagaaag gcagtgtcgc gcgggttagt cgtcaaggtt
    11341 tcatttcgat aatcccccat cccgccaata tttaagccca gactgctttt acttccgcta
    11401 ccgtctgatt gggtgcgaga gagtgcaggg gcattaagtg tagataaaga agtggttaaa
    11461 cctgtcaaac cgcctgatgt tgtttcagcg gtggtttcgt aacgtggata ggtgtaatcg
    11521 gtacggacgg cagggctgtt tatgtattcg ccacgaatca gtgcatcaat ggagtagcga
    11581 cccccctgtc aaactgcctg aaccttggtc gcccatagtg gcaatgtaca atgcaacttt
    11641 tcgtccgtgt aatgcctgta aatccatgtc tttaacggca gctctggcag aagcggccac
    11701 aagttcttgt tcgaccgcaa agcgtttacc tccgccatgc gatggaatac ctgtcagtgt
    11761 cccgcaggcg gataaaataa aaactgaaaa aagaataggt atcagcagcc gtgcttgcat
    11821 agattttctc ctttgatgaa aaacaaattg tatcaaaatt gtaaatatag tggattaaat
    11881 ttaaaccagt acggcaaggc gaggcaacgc cgtactggtt taaatttaat ccactataat
    11941 ctccatataa taaatggctg tctgaagccc atatgcgact atatctgccg acactatggt
    12001 caatcagcag tgaaccagcg ttccctcgtc ttcgccggta atgacgcgtt tgagcgagcc
    12061 ttctttggcg atgccgaaga cgacaatatt gagcttgcgt tcgcggcaga gggcgaaagc
    12121 ggtcgcgtcc atgactttga ggtttttcaa caaggcttcg tcaaaagtaa tggtttcgta
    12181 gcgcgtggcg gacgggtctt ttttcgggtc tgcggtgtac acaccgtcga cgttggtggc
    12241 tttgagcatc acgtcgcagt tcatttccgc accgcgcaat gcggcggcag tgtcggtcgt
    12301 gaagaacggg ttaccggtac cggcggcaaa aatcacgact ttgccttctt ccaaatattg
    12361 gatggctttg gggcgggcgt aggtttcagc gatttgctgc atagacagtg cggattgtac
    12421 gcgcgctttg atgcctaaag tttcaaatgc gtctttgagt gccaacgcgt tcatcacggt
    12481 cgccatcatg cccatgtagt cggcggtggc gcgatccatg ctgcctgctt gggcggatac
    12541 gccccggaaa atattgccgc cgccgacaac aataccgact tgcacgccca ttttaacgac
    12601 ttcggcaatt tcgccgacag tttgaacgat ggtatcgtga ttgatgccga acggatcgga
    12661 acccatcagg gattcgccgg agagtttcag taatacgcgt ttgtatttga tttgctgtgt
    12721 catggatacc ttgctttctt gaatgtcgtc ggtcggagtt gggccgtctg aatttccaac
    12781 ggtcggaata ttaatgatag ggtgtgtcgg tttattattg ttttgtttta agacggtctg
    12841 aatattcggt cgtcttaaaa taggtaccgt aaacggtttt ctcaaaaaaa agcaccctga
    12901 ttcgtttgga atccaggtgc tttctttttc ataagtgcct tacactttag cagcggcagc
    12961 aacttcggct gcgtaatcga cggctttttt ctcaatacca tcgcctactt tgtagcgtac
    13021 gaagctgatc acttcagtgc cgttttcttt agagaattgg gcaacagttt gatcggggtt
    13081 catcacgaat gcttggccgt tcagagtgat ttcagccaag aatttacgga tgcggccttc
    13141 aaccatttta gcggcgatgt cggcaggttt gccggaagcg atggcttgct cggtgtagat
    13201 gtggcgttct ttttcaacgg tttcggcatc tacttcggct tcgcttacgc attgtggttt
    13261 agcggcaaca atatgcatac cgattttgcg tgctacgtct tcagagcctt tgtactcaac
    13321 caatacgcct tcggtcgcca atgcgccgtg gatgtaggca accagttggt tggcagtgtc
    13381 gatcacttgg aagcgacgga cagacatatt ctcgcccaat ttggcgatga tggctttgcg
    13441 ttctgcttca accagttcgc tcagttcttc aacagaagcc ggttttttct cggcagcagt
    13501 tttcgcaacg aagttggcaa attctacgaa gcccgcgtct ttagcaacga agtcggtttc
    13561 gcagtttact tcgaccaatg cgccgacatt gccgttgatc gcgtaagcca atacgccttc
    13621 ggcagcggta cggccggcca gtttaccggc tttcgcaccg gatttgatac gcaggatttc
    13681 ttcggctttg tcgaagttgc cttcggcttc aaccaaggct tttttgcatt ccatcatgcc
    13741 caggccggta gcggcgcgca ggtcggcaac catttttgca gtaatttctg ccattttgaa
    13801 tctcctagat ttttgggaaa tacggaaagg attttccgca ttcgggaatc gtgtgccgcg
    13861 aacggacgtt cagacggcat atttgagaaa aggggcataa cgcccctctt cggtttgccg
    13921 gattactcgg cagcggcttc ttgggcagcg gctacggttt cttgcagcgc ttggtttttg
    13981 ccttccaaaa ctgcgtcagc gatgccgcgg cagtacaggc ggatggcttt ggcggagtcg
    14041 tcgttgccgg ggataacgta tttcacgccg tcggggctgt tgttggtatc gactacggcg
    14101 ataacaggga tgcccaattt ttcagcttca accagagtac ctttttggta gccggtatcg
    14161 ataacgaaaa tcgcgtcagg caggcctttc atgtttttga taccgcccaa agaacgttcc
    14221 agtttttcaa catcgcgttg catttccaga atttcttttt tgctgaaacc gctttcggca
    14281 gcattttcca aggctgcggt tttttcttcc aggcgtttga tggattgctt aacggttttg
    14341 tagttggtca gcataccgcc caaccagcgg taatcgacga aaggcatacc ggcgcgggtc
    14401 gcttcttcgc ggatgatgtc gcgggcttgg cgtttggtac ctacgaacaa tactgtacct
    14461 ttgttggcaa ccagacgacg tacggcttct tgcgcgtctt ggaacatcgg cagggttttt
    14521 tccaggttga cgatatggat tttgttgcgc gcaccgaaaa tgtattgtgc cattttcggg
    14581 ttccagaaac gggtttggtg gccgaagtga acaccggctt caatcatctg acgcatagta
    14641 atttgagaca ttttatttcc ttgaaagggt taagagcaca cattcaatcg catagaactt
    14701 gataggtcac gcctattgtc aagcaccctt cggcgaaagt gggcataagt tttaaaaaat
    14761 agtgtgtttc cgaaatggaa aactgacgga ttatagcgga taagatgccg tctgaaaagg
    14821 gattcagggt tttgtgccgc tgtctttttg ccgttgtgcg cgctgctgtt cggattccgc
    14881 ctgttcctgc tgcctcatca ggtggttgcg gcggcggacg gtaatggtga aaaccgtgaa
    14941 cacggtgggc agcaccgccc aaaaaaccaa ataaatcaag gcgcgcgcaa tactcggctg
    15001 cgcggcggaa tacataacgg caacaaaaag atagccgata gcgacgatgt gccacattgt
    15061 gtgcgtcctg tttgaatgtt aacaaaatcg ttataatcgc aacattctac cgcaatcctg
    15121 aaaggccgtc tgaaatgagg aacgaggaaa aacgcgccct gcgccgcgaa ttgcgcgggc
    15181 ggcgttcgca aatggggcgg gacgtgcggg cggcggcaac ggtaaaaatc aaccacctgc
    15241 tcaaacgtta tattaaaaaa gggcggaaaa tcggcgtgta ttggccgatg ggcaaggaat
    15301 tgcgtttgga cggctttgtc cgcgcggcgc aaaaacgcgg tgcggaactc tacctgcctt
    15361 atatcgaacc gcgttcgcgg cggatgtggt ttacgccgta tcctgccgat ggagtaaaac
    15421 aagaacgcaa gcgcggtagg gcgaagctgc atgtccctca gtttgcaggt cggaaaaagc
    15481 gtgtgcatga tttgaacctc ctgcttgtgc cagtggtcgg tatggacagg ctgggctacc
    15541 gcttgggaca ggcaggcggc tattacgatg cgacgctttc agcgatgaaa taccgtttgc
    15601 aggcaaaaac cgtgggcgtg ggctttgcct gccagttggt ggacaggctg ccggtcgagg
    15661 cgcacgaccg gtctttggac ggttttgtgt cggaggcggg gatattgtgt ttttagccgt
    15721 tcagacggca ttccgggcgg aaatgccgtc tgaaggcgga atattgggag aaaaggatgt
    15781 cggaaaagat aatactgccg gtcttgtcgt tgggcggcgt tcgtctcgaa ccgttggatg
    15841 tgcatcatga aacgggtttg cgcgaggcgg tttgcgatgg ggaggtttgg aagctggggg
    15901 taacgtccgc gccccatccg gatagggtgg cagactatat cgggacagca ttggcaacgc
    15961 gtttggcgtt tgcggttgtc gatgaagagg cgggcagggt ggtcgggaca acggcgtatt
    16021 atcattttga accgcagatt ccgcgtttgg atatcggatt cacatggtat gcggcatcgg
    16081 cacggcggac acggataaac acctgttgca aaatcatgct gttggattcc gcatttgatg
    16141 ttttggcttg ccgttgcgtg ggatggcgca cggatattct gaaccttgcc tcgcaacgtg
    16201 ccatcgaacg gctgggtgcg gaaaaagacg gcgtgctgcg tatgcatatg ctccgtaaag
    16261 acggcagtgt gcgcgatacg gtcgtgtaca gtatgctgag ggaagattgg tgcaaaaaca
    16321 gggaaatcct gaccggcagg ttggcagggt atggcgtgca ggtgtgaggc agtattttca
//
LOCUS       DAWIVB010000028        16367 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWIVB010000028 DAWIVB010000000
VERSION     DAWIVB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.028"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            125..1189
                     /locus_tag="WIX60_000653"
     CDS             125..1189
                     /locus_tag="WIX60_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ5304953.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVAEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATINVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1330..1653
                     /locus_tag="WIX60_000654"
     CDS             1330..1653
                     /locus_tag="WIX60_000654"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ5304954.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLVEKA
                     ARTES"
     gene            1725..2048
                     /locus_tag="WIX60_000655"
     CDS             1725..2048
                     /locus_tag="WIX60_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ5304955.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQMLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2095..2298
                     /gene="tatA"
                     /locus_tag="WIX60_000656"
     CDS             2095..2298
                     /gene="tatA"
                     /locus_tag="WIX60_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ5304956.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2302..2988
                     /gene="tatB"
                     /locus_tag="WIX60_000657"
     CDS             2302..2988
                     /gene="tatB"
                     /locus_tag="WIX60_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ5304957.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3002..3772
                     /gene="tatC"
                     /locus_tag="WIX60_000658"
     CDS             3002..3772
                     /gene="tatC"
                     /locus_tag="WIX60_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ5304958.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            3825..4433
                     /gene="maf"
                     /locus_tag="WIX60_000659"
     CDS             3825..4433
                     /gene="maf"
                     /locus_tag="WIX60_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ5304959.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4442..4795
                     /locus_tag="WIX60_000660"
     CDS             4442..4795
                     /locus_tag="WIX60_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ5304960.1"
                     /translation="MNRQTAYLLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4785..6599
                     /locus_tag="WIX60_000661"
     CDS             4785..6599
                     /locus_tag="WIX60_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ5304961.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAIERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPSHPEAKPCK"
     gene            6789..7466
                     /locus_tag="WIX60_000662"
     CDS             6789..7466
                     /locus_tag="WIX60_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ5304962.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7480..8886
                     /locus_tag="WIX60_000663"
     CDS             7480..8886
                     /locus_tag="WIX60_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ5304963.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9004..11430
                     /locus_tag="WIX60_000664"
     CDS             9004..11430
                     /locus_tag="WIX60_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ5304964.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASEQPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDNLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLVSFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLHAHAPKNLTLHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLAHPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFLLKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11475..11720
                     /gene="rpsP"
                     /locus_tag="WIX60_000665"
     CDS             11475..11720
                     /gene="rpsP"
                     /locus_tag="WIX60_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ5304965.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11736..12245
                     /gene="rimM"
                     /locus_tag="WIX60_000666"
     CDS             11736..12245
                     /gene="rimM"
                     /locus_tag="WIX60_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ5304966.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12245..12994
                     /gene="trmD"
                     /locus_tag="WIX60_000667"
     CDS             12245..12994
                     /gene="trmD"
                     /locus_tag="WIX60_000667"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ5304967.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKTQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13010..13375
                     /gene="rplS"
                     /locus_tag="WIX60_000668"
     CDS             13010..13375
                     /gene="rplS"
                     /locus_tag="WIX60_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ5304968.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13696..14421
                     /locus_tag="WIX60_000669"
     CDS             13696..14421
                     /locus_tag="WIX60_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ5304969.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMQRFADRQIAYLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14445..15320
                     /locus_tag="WIX60_000670"
     CDS             14445..15320
                     /locus_tag="WIX60_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ5304970.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15397..16311
                     /locus_tag="WIX60_000671"
     CDS             15397..16311
                     /locus_tag="WIX60_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ5304971.1"
                     /translation="MKHLKLTLIAALLTASATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYTEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4237 a         5249 c         3606 g         3275 t
ORIGIN      
        1 tcttgtagta aaatatctaa acagattcaa ctggaaacgg tcaagcttct tcacgccatt
       61 cccatttcaa ccgccgcctg ccagattcag acggtattcc tacttgaaaa caaggagcgt
      121 tacaatgaaa gcagcacgtt tttacgacaa aggcgacatc cgcatcgaag acatccccga
      181 accgaccgtc gcccccggca ctgtcggcat caatgtcgcc tggtgcggca tctgcggtac
      241 tgacctgcac gaattcatgg aaggcccgat tttcattccg ccttgcggtc atccgcaccc
      301 gatttccggc gagtccgcac ccgtaacgat gggacacgag ttctccggcg tggtctatgc
      361 cgtcggcgaa ggcgtggacg acatcaaagt cggccaacac gtcgtggtcg aaccctacat
      421 catccgcgat gacgtaccga ccggagaagg cagcaactac cacctctcca aagatatgaa
      481 ctttatcggc ttgggcggct gcggcggcgg tctgtccgaa aaaatcgccg tcaaacgccg
      541 ttgggtgcat ccgatttccg acaaaatccc gttggatcaa gccgctttga tcgaaccgct
      601 gtctgtcgga caccacgcct atgtacgcag cggcgcgaaa gaaggcgacg tcgcattggt
      661 cggcggtgca ggtccgatcg gtttgctgtt ggctgccgtg ttgaaagcca aaggcatcaa
      721 agtcatcatc accgagttga gtaaagcacg caaagacaaa gcgcgcgaat ccggcgttgc
      781 cgactacatc ctcgacccgt ccgaagtcga tgttgttgca gaagtgaaaa aactgaccaa
      841 cggcgaaggc gtggacgtgg catttgagtg caccagcgtc aacaaagtgt tggatacttt
      901 ggtcgaagcc tgcaaacctg ccgccaattt ggttatcgta tccatctgga gccaccccgc
      961 caccatcaac gtccacagcg tcgtgatgaa agagttggac gtgcgcggca cgattgccta
     1021 ctgcaacgac cacgccgaaa ccatcaaact ggtcgaagaa ggcaaaatca accttgagcc
     1081 tttcatcacc cagcgcatca agctggacga gctggtttcc gaaggcttcg agcgtctgat
     1141 tcacaacaac gaatccgccg ttaaaattat tgtgagtcca aacctgtaag caaattacat
     1201 ttgcaacagc acaaatgccg tctgaaacgc tcagacggca ttttccaacg aagcgcaaca
     1261 ggcgtattgc ccgagagcag ccgtttagcc tatcattccg aacaaacaga aacaaacaga
     1321 aggaaaatca tgggagattc cgtactatcc gccatccaac aaaccatcac ccagcgaaaa
     1381 tctgccaatc cgtccgaatc ttacgtcgca cagctcttgc ataagggcga agacaaaatc
     1441 ctaaaaaaag tgattgaaga ggcgggcgaa gtgttgatgg catccaaaga caaaaacccg
     1501 tcccacctgg tttacgaagt tgccgactta tggtttcaca ccatgattct tctgacacac
     1561 cacgacctga aggcggaaga cgtattggac gaacttgcgc gccgtcaggg gctgtcgggg
     1621 ctggtcgaaa aagccgctcg cacagaatct tgaatttata ttaaaatccg cactttccca
     1681 cattcaatcc gtctgaccgc ttttcagacg gcatcggagc cgttatggac aactgtattt
     1741 tctgcaaaat cgccgccaaa gacattccgg cgcaaaccgt ctatgaagac ggcgaaatgg
     1801 tttgtttcaa agacatcaac cccgctgctc cggttcatct gctgctgatt cccaaagtcc
     1861 atttcgattc gttggcacac gccgcgcccg aacatcagat gctgctgggc aaaatgatgt
     1921 tgaaagttcc cgaaatcgcc aaagcggcag gactggcaga cggcttcaaa accctgatca
     1981 ataccggaaa aggcggcgga caagaggtct tccacctgca tatacacatc atgggtacac
     2041 ccgtataaac cgttatttca caatcaaccc ctaatactta cttaaggata catcatgggc
     2101 agtttttctc tgacgcactg gattatcgta ctgattatcg tcgttttgat attcggcacc
     2161 aaaaaactgc gcaacgtcgg caaagacctc ggcggtgcgg ttcatgactt caaacagggg
     2221 ctgaacgaag gtacagacgg caaagaagcc caaaaagacg atgtaatcga acacaaaaaa
     2281 gacgaagaca aagcgtaatt tatgtttgat ttcggtttgg gcgagctggt ttttgtcggc
     2341 attatcgccc tgattgtcct cggccccgaa cgcctgcccg aggccgcccg caccgccgga
     2401 cggctcatcg gcaggctgca acgctttgtc ggcagcgtca aacaggaatt tgacactcaa
     2461 atcgaactgg aagaactgag gaaggcaaag caggaatttg aagctgccgc cgctcaggtt
     2521 cgagacagcc tcaaagaaac cggtacggat atggaaggca atctgcacga catttccgac
     2581 ggtctgaagc cttgggaaaa actgcccgaa cagcggacac ctgccgattt cggtgtcgat
     2641 gaaaacggca atccgcttcc cgatgcggca aacaccctat cagacggcat ttccgacgtt
     2701 atgccgtccg aacgttccta cgcttccgcc gaaacccttg gggacagcgg gcaaaccggc
     2761 agtacagccg aacccgcgga aaccgaccaa gaccgcgcat ggcgggaata cctgactgct
     2821 tctgccgccg cacccgtcgt acagaccgtc gaagtcagct atatcgatac tgctgttgaa
     2881 acgcctgttc cgcacaccac ttccctgcgc aaacaggcaa taagccgcaa acgcgatttt
     2941 cgtccgaaac accgcgccaa acctaaattg cgcgtccgta aatcataaag agagcaatcc
     3001 ggtgtccgaa acacaaaacg aacaacccgt ccaaccgctt gtcgagcatc tcatcgagct
     3061 gcgccgccgc ctgatgtgga cggttgtcgg catcttagtc tgctttttcg gcctaatgcc
     3121 gtttgcccaa caactctata cttttatcgc cgacccgctg atggcaaacc tgcccaaaga
     3181 caccagcatg attgccaccg atgtcatcgc accatttttc gtgccggtca aagttaccct
     3241 gatggcggca tttttaattt cgctgccgca tacgctctac caaatctggg catttgtcgc
     3301 gcccgcactc taccaaaacg aaaaacgcct gattacgccg ctcgtcctct ccagcgtcag
     3361 cctgtttttc atcggcatgg catttgccta ctttttggtt ttccccgtca ttttcaaatt
     3421 ccttgccagc gttacccctg tcggtgtcaa tatggcgaca gacatcgaca aatacctctc
     3481 cttcatcttg gggatgtttg ttgcgttcgg cacaacgttt gaagtcccca ttgtcgttat
     3541 cctgttaacc aaaattggtg tggtaacaac cggacagctc aaacgcgccc gcccctatgt
     3601 gattgtcggc gcgtttgtca ttgccgccat catcacgccg cccgatgtga tttcacaaac
     3661 cctgcttgcc attccgctga ttctcttata cgaagcaggt atttggttcg gacgcttttt
     3721 cacgccacgt tcagaacagg atggcgacat acggccgcct gcaacaacct gacactatgc
     3781 cgtccgaacc tccgcctcat accgcaacag attaaggaat acctttgaat accctctatt
     3841 taggttcaaa cagcccgcgc cgaatggaaa tcctgacaca gttgggctac cgcgtcatcc
     3901 aactgcctgc cggcatcgac gaatccgtta aagccggcga aacacctttc gcttacgttc
     3961 aaaggatggc agaagaaaaa aaccgaaccg ccctgaccct cttttgcgaa accaacggca
     4021 caatgcccga tttccccctg attaccgccg acacctgcgt cgtttcagac ggcatcatat
     4081 tgggcaaacc ccgctcccaa gccgaagcaa tcgaattttt aaaccgattg tccggcaaac
     4141 aacataccgt cctgactgct gtctgcattc attatcgcgg caaaacgtca agccgcgtcc
     4201 aaaccaaccg cgtcgttttc aagcccctga gttcggaaga aatttccgcc tatgtgcaaa
     4261 gcggcgagcc gatggacaaa gccggtgcct acgccgtaca aggcataggc ggcatcttta
     4321 tccaatctat cgaaggcagc ttcagcggca ttatgggact gcccgtttat gaaaccgttt
     4381 cgatgttgca ggatttggga taccgctccc ccttgtccgc ccttaaaccg taaagaccgc
     4441 cgtgaacaga caaaccgctt acctccttgc ctctttcagc ctgatcgcac tgataatcct
     4501 gtccctttcc tgggaactgt ggattgcacc gttgcgcccg ggcggctcgt ggctcgcgct
     4561 caaagccctc cccctctgtc tgccgctttc aggcatcttg aaaaagaaaa tctatactta
     4621 ccaatacagc tccatgctgg ttctgattta ctttgccgaa gccgtcatgc gcctgttcga
     4681 cgcctatccc gcagaacaga tttgcgccgc gctttccgca gtattcagca tcatcttctt
     4741 catatcctgc ctgtcgttcg tcaaacaata caaggaaaca aacaatgccc gctgaaacga
     4801 ccgtatccgg cgcgcacccc gccgccaaac tgccgattta catcctgccc tgcttccttt
     4861 ggataggcat cgtccccttt accttcgcgc tcaaactgaa accgtcgccc gacttttacc
     4921 acgatgccgc cgccgcagcc ggcctgattg tcctgttgtt cctcacggca ggaaaaaaac
     4981 tgtttgatgt caaaatcccc gccatcagct tccttctgtt tgcaatggcg gcgttttggt
     5041 atcttcaggc acgcctgatg aacctgattt accccggtat gaacgacatc gtctcttgga
     5101 ttttcatctt gctcgccgtc agcgcgtggg cctgccggag cttggtcgca cacttcggac
     5161 aagaacgcat cgtgaccctg tttgcctggt cgctgcttat cggctccctg cttcaatcct
     5221 gcatcgtcgt catccagttt gccggctggg aagacacccc tctgtttcaa aacatcatcg
     5281 tttacagcgg gcaaggcgta atcggacaca tcgggcagcg caacaacctc ggacactacc
     5341 tcatgtgggg catactcgcc gccgcctacc tcaacggaca acgaaaaatc cccgccgccc
     5401 tcggcgtaat ctgcctgatt atgcagaccg ccgttttagg tttggtcaac tcgcgcacca
     5461 tcttgaccta catagccgcc atcgccctca tccttccctt ctggtatttc cgttcggaca
     5521 aatccaacag gcggacgatg ctcggcatag ccgcagccgt attccttacc gcgctgttcc
     5581 aattttccat gaacaccatt ctggaaacct ttactggcat ccgctacgaa actgccatcg
     5641 aacgcgtcgc caacggcggt ttcacagact tgccgcgcca aatcgaatgg aataaagccc
     5701 ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg gaacagtttt gcccaacaaa
     5761 ccttcctcat caatgccgaa cagcacaaca tatacgacaa cctcctcagc aacttgttca
     5821 cccattccca caacatcgtc ctccaactcc ttgcagagat gggaatcagc ggcacgcttc
     5881 tggttgccgc aaccctgctg acgggcattg ccgggctgct taaacgcccc ctgacccccg
     5941 catcgctttt cctaatctgc acgcttgccg tcagtatgtg ccacagtatg ctcgaatatc
     6001 ctttgtggta tgtctatttc ctcatccctt tcggactgat gctcttcctg tcccccgcag
     6061 aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct cggcatactg accgcctccg
     6121 ccgccatatt cgcaggattg ctgcacttgg actggacata cacccggctg gttaacgcct
     6181 tttcccccgc cactgacgac agtgccaaaa ccctcaaccg gaaaatcaac gagttgcgct
     6241 atatttccgc aaacagtccg atgctgtcct tttatgccga cttctccctc gtaaacttcg
     6301 ccctgccgga ataccccgaa acccagactt gggcggaaga agcaaccctc aaatcactaa
     6361 aataccgccc ccactccgcc acctaccgca tcgccctcta cctgatgcgg caaggcaaag
     6421 ttgcagaagc aaaacaatgg atgcgggcga cacagtccta ttacccctac ctgatgcccc
     6481 gatacgccga cgaaatccgc aaactgcccg tatgggcgcc gctgctaccc gaactgctca
     6541 aagactgcaa agccttcgcc gccgcgccca gtcatccgga agcaaaaccc tgcaaatgac
     6601 ccgcgccggc ggatgcggat accgcccgaa atgtaaagct ccatgcaaga cattgcaaaa
     6661 aacagcaaac cggtagggaa atacgctatc aaaaaacatt gcagccgtgt taagataaac
     6721 cgtcaaacaa tcttttcacg ccccgcccga aacagggtcg gggcataccc ttacgaaaag
     6781 gaaacaccat gagccgcgta ttactcgtag atgacgatgc cctgctaacc gaactgctga
     6841 ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt tcccgacggg gaagcaggcg
     6901 tacaggaaat cctgagcggg cagtacgatg tagtcgtatt ggattccatg atgcccaaaa
     6961 tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag caccgtcccc atcatcatgc
     7021 tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt ggaaatgggc gcggacgact
     7081 atgtccccaa accctgcaca ccacgcgaac tcttggcacg catcaatgcc atcctgcgcc
     7141 gcgcacaaca cagcggcgaa cagaacaacg cacccaacag catctccgtc agcgatgtcg
     7201 tcctgtaccc cgccaaacgc caggcatccg tcaaagacat gccgctcgaa ctgaccagca
     7261 ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg acaggtagtc agcaaagaaa
     7321 ccctgtccgt cgaagcactc gaccgcaagc tggcaaaatt cgaccgcagt atcgacgtac
     7381 acatctccag catccgccac aagttgggcg atgcctctct gattcaaacc gtacgcggct
     7441 tgggctacct gtttgtcaaa aactgaaata aacagataaa tgaaactgtt ccaacgcatt
     7501 ttcgccacat tttgcgcggt tatcgtctgt gcaatctttg tggcgagttt ttctttctgg
     7561 ctggtgcaga acacccttgc cgaaaaccag ttcaaccaac gccgcaccat cgaaaccact
     7621 ttgatgggca gcatcatttc cgcattccgg gcacgcgggg acgcgggtgc gcgcgaaatc
     7681 ctgacggaat ggaaagacag ccccgtctca tcgggcgtgt acgttataca gggcgacgag
     7741 aaaaaagata tcctgaaccg gtatatcgac agctatacca tcgaacgcgc ccggcttttc
     7801 gccgccggac acccgcattc caacctcgtc catatcgaat acgaccgctt cggcgaagaa
     7861 tacctgttct tcaccaaaga ctgggacaaa ctccaagccc gccgcctgcc cagccccctg
     7921 ttgatccccg gcctgccgct cgccccgatt tggcacgaac tcatcatatt gtccttcatc
     7981 atcatcgtcg gactgctgat ggcatatatc ctcgccggca acattgccaa acccatcaga
     8041 atcttaggca acggcatgga cagggtggca aacggagaac ttgaaacccg tatctcccaa
     8101 caggtcgacg accgcgacga cgaattgtcc catcttgcca tccaattcga caaaatggtg
     8161 gaaaaactcg aaaaactcgt tgccaaagaa cgccacctgc tccatcacgt ctcccatgaa
     8221 atgcgttctc cccttgcgcg catgcaggca attgtcggac tgattcaggc gcagccccaa
     8281 aaacaggagc aatatctcaa acggctggaa ggcgaactga cccgcatgga tacgctggcc
     8341 ggggaactgt taaccctgtc ccgtctcgaa acttccaata tggctttgga aaaagaaagc
     8401 ctgaaactcc tgcccttcct gggcaacctg gtagaagaca atcaaagcat tgcccagaaa
     8461 aacggacaaa cggttaccct gtctgccgac ggaaaaatcc ccgaaaacac aaccatcctt
     8521 gccaacgaaa gctacctgta ccgcgccttc gacaacgtca tccgcaacgc cgtcaactac
     8581 agtcccgaag gcagcaccat cctgatcaac atcggacaag accacaaaca ctggataatc
     8641 gacgttaccg acaacggccc cggcgtggac gaaatgcagc tcccgcacat cttcaccgct
     8701 ttctaccgtg cagactccag tgccaacaaa cccggaacag gactggggct tgcattgacc
     8761 caacatatta ttgaacagca ctgcggcaaa atcatcgccg aaaacatcaa accgaacggt
     8821 ctgcggatgc gctttatcct gcccaagaaa aaaaccggtt ccaaaacaga aaaaagtgcg
     8881 aactgaccat aataccgtct gaaccggctt cagacggcat tgcacaaaca gttaccccaa
     8941 aacaaacaca cagcgggtgt ctctccaaaa atcacgcaca taccgtccgc aaaggaatat
     9001 atcatgtcgg cacaaaccga tccgggctac ttcttcatgc ccaaccacat catcctgata
     9061 ggcgcgagcg aacaaccgta cagcctgggc gaacgtgtac tcagcaacct gctgagtacg
     9121 ccctttcaag gaaaaatcac ccccgtaaac ccgcgccacc acaccatagc cggactgccc
     9181 gcctacacca gcctcaacaa aatccccggc aatgcagacc tgattattgc cgttacccta
     9241 cccgacagtt acgacaccct cttcaaaacc tgccgcaaaa agcagctccg acacatcatc
     9301 ctcatacagg actgggacaa cctgtctgcc gcagaactgc acaccgccga aactgccatc
     9361 cgcaaacacc acggcaacgg actcaacatc accgcctgca ccaccgcagg catccaactg
     9421 ccctcactcg gactcaacat cagtacccaa gacggatacg ccgcaggcca taccgccata
     9481 ctgaccggca atgccgccgt cagccgccaa atcgacaaca tcctgaacaa actccgtcaa
     9541 ggcacatccc gccacatcag cctgcatccc ggcatcagcc ccatcacatc cgccgattgg
     9601 ctcaaccgct tcggacacag cctgcacacc aaaaccgccg tcctacacca caaccctgaa
     9661 gaggatcagc gcaaactgtt cagcgcaatc cgccaattta cccgccatac gccgctgatt
     9721 ctccacatca cctgccgcac gacagaaacc gaccgtgccg tactgcactg cctcgcccga
     9781 cactgcaact tcctcgtcag tttcaacgcc gacgacctcg aagccgcact gcgcgcccaa
     9841 ctgtccgacc ttccacccct gtcccgactc gacatcctgt ccgacacgcc tgccgaatgg
     9901 ctgcacgcgc acgcgccaaa aaacctcacc ctccactttc ccaaccttcc ccaccacatc
     9961 cgcaacggac acctgaccgg cacacccaca ccctcaatct gccacgacat cgcctcacgt
    10021 cagcttgccc accccgacac ccaagccgtc ctaaccatcc tcagtccctc cggacacgag
    10081 gattacaaaa aaacagcacg cgcccttatc cgcctgtccg aacagaccgc caaacccctg
    10141 ctcgtcagca gccccttttc agacggcata acccatttcg acacccccac tcaggcaatc
    10201 cgcacccttt cctaccgcaa caccgccgcc gccctgaaac aggcacagct cgacattgca
    10261 ccgccgcagc catgccgtct gaaaacaccg caaccccaaa acatcaaaaa agcccttgca
    10321 gcggcaaacc cctccctgct cgccgaagcc ctgcacctcc ccccctaccg gcacaccacc
    10381 cataacgccg tacaattcca attcgacagc caccccctct atggcgacat cctgaccgca
    10441 cgctgcaacg gacaaaccac tgccgtactc ccgccgttta ccacgctcga cagccgccac
    10501 cttgcccgct ttgccgaact cgacggcaca caaaccctcg accagttcct gcacacactg
    10561 accgtcattc ccgaataccg ccaacacatt ctcggcatca ccctcaacct caacggcgga
    10621 caatacagca gcgatttcct cttaaagaca cccgaaacac acgacacgcc caaacgcaag
    10681 aacacaggca aagccgccca aaccctcgaa catgccgccg caaaaatgca gagtgccgcc
    10741 gcatacctga aacacaaaaa cccgacagcc gccgaatttc tccgccacac aagcgaagcc
    10801 gccgcagaac tgctcggcag caaaaccgaa accggagcag ccgtacccaa cgtacttgcc
    10861 ccctatcccg cagcataccc caaaacactg tccctaagaa acaacacgac cgttaccatt
    10921 acccccattt tgcccgaaga cgcagaagcc aaacagcagt tcgtccgcag cctcggtccc
    10981 gaagcacggt acacacgctt catgacccac accaacgaac tgcccgcagc cacgttggca
    11041 cgcctgtgca accccgatta ccactgtgaa gccgcatgga cggcaaagga tgccgacagc
    11101 aacatcgtcg ccgtcgtccg ccacagccgc ctgaatcgca acgaatgcga attcggcatc
    11161 acactggcgg aacatatgcg cggcagcgga ctggcacaga aaatgatgga actcatcatc
    11221 caaaccgccg cacagcaagg ctaccggact atgagtgccg acattctcaa aaccaatacc
    11281 cccatgatca aacttgctga aaaatcagga tttaccctca aggaatcgga caccgaaaaa
    11341 aacctgtacc gcgcatatct gaaccttgcg gcagacaaaa caacagaaaa aacaaataaa
    11401 aacttgcgca ccgaccacaa aataacctaa aatcggcagt ttccatatat ccgattttct
    11461 caaaaggact caaaatggta gttatccgtt tggcacgcgg cggctcgaaa caccgcccct
    11521 tctacaacgt catcgttact gactcacgca gccgccgcga cggccgcttc atcgaacgcg
    11581 taggcttcta caaccccgta gccaacgaaa aacaagagcg cgtccgcctc aatgcagacc
    11641 gcctgaacca ctggattgca caaggcgcgc aagtcagcga ctccgttgca aaactgatta
    11701 aagaacaaaa agccgcctaa tccgcatttg ccgccatgac agacactcaa aaccgggtag
    11761 ccatgggcta catcaaaggc gtattcggca taaaaggctg gttgaaaatt gccgccaaca
    11821 ccgaatattc cgacagcctt ttggactacc ccgagtggca tttggtcaag gacggcaaaa
    11881 ccatcagcgt tacccttgaa gccggaaaag tcgtcaacgg cgaactccaa gtcaaattcg
    11941 aaggcataaa cgaccgcgac ttggcattct cattgcgcgg ttacaccatc gaaatacccc
    12001 gtgaagcatt cgccccgaca gaagaagacg aatactactg gacagacttg gtcggcatga
    12061 ccgttgtcaa caaagaccat accgttttag gcaaggtaag caacctgatg gaaaccggcg
    12121 caaacgacgt attgatgatt gacggagaac acgggcagat tctgattccg ttcgtttccc
    12181 aatatatcga aaccgtcgat accggcagca agaccattac tgccgactgg ggtttggact
    12241 actgatgctt atccaggcag ttaccatttt ccccgaaatg ttcgacagca ttacccgcta
    12301 cggcgtaacg ggacgcgcga acagacaggg aatctggcag tttgaagcag tcaatccccg
    12361 aaagtttgcc gacaacagat tgggctacat cgacgaccgc ccgttcggcg gcggcccggg
    12421 aatgattatg atggctccgc cgcttcatgc ggcaatagaa cacgccaaaa cacaatcctc
    12481 ccaagctgca aaagtcatct acctcagccc ccaagggaaa ccgctgacac accaaaaagc
    12541 ggtagaactg gcagaacttc cgcatctgat tctgctgtgc ggacggtatg agggcataga
    12601 cgaaaggctt ctgcaaagca gcgtcgatga agaaatcagc atcggagact tcgttgtttc
    12661 cggcggagag cttcccgcca tgatgctgat ggatgcggta ttgaggctcg tacccggcgt
    12721 attgggcgat atgcagtctg ccgaacagga ttcgttctca agcggcattt tggactgccc
    12781 ccactacacc aaacccttag aatttcaagg tatggctgtt ccggaagtat tgcgttccgg
    12841 caatcatggc ttgatagcgg aatggcggtt ggaacaatcg ctgcgccgca ccttggagcg
    12901 cagacccgat cttttggaaa agcgcgtttt aatcccaaag gaatcccgcc tcttagaaac
    12961 catccggcaa gagcaacggg aaatccaatc ataatttagg aaaaaaacaa tgaacctgat
    13021 tcaacagctc gagcaagaag aaattgcccg cctgaataaa gaaatccccg aattcgcacc
    13081 gggcgacacc gtagtcgtat ccgtacgcgt cgtggaaggt acccgcagcc gtctgcaagc
    13141 ctacgaaggc gtggttattg cccgtcgcaa ccgtggtctg aacagcaact tcatcgtccg
    13201 caaaatctcc agcggcgaag gtgttgaacg tactttccaa ctgtactctc cgaccgtcga
    13261 aaaaatcgaa gtcaaacgcc gtggcgacgt acgccgtgcc aaactgtact acctgcgcgg
    13321 cctgaccggt aaagctgcac gcatcaaaga aaaactgcct gcacgcaaag gttgattcaa
    13381 accgttttcc cccaatgccg tctgaacctt cagacggcat ttcttattgc tgcgatgccg
    13441 gcaagtcaac gatatgcctg cccctcatcc accttgatta acaatcactc ccgcctattc
    13501 aagcataatt tacagaccaa acgttataca gtatcatatc agccatataa cgataaacgc
    13561 tttaccgcct tccctttcaa aaccgcaaat catcatgaca ccttcccttt tactatcagg
    13621 attgaccttc cgcctcatcc ttgccctgat tgccgtatcc cttttatggg gcgtttactt
    13681 ttgggcagta tccgcatgag catcattgtc gaaaacctga ccgtcagcta ccgccgccga
    13741 cctgccgtgc accatgtgga cattactttt gaagaacata gtatgtgggc ggttttcggt
    13801 cccaacggcg cagggaaatc cacctttctc aaatccttga tgggattgca gcctatcgat
    13861 acaggcagca tccggctgga cggattgacc cgtcagaaca tcgcctacct tccccagcag
    13921 tccgatatcg accgctccca gcctatgacc gttttcgact tggcggcaat ggggctatgg
    13981 tatgaaatcg gctttttcaa agggataaat accgctcaaa aacaacgcgt tcacgaagcc
    14041 ttggagcgcg tcggaatgca acggtttgcc gaccgtcaga ttgcctatct ctcaaacgga
    14101 caatttcagc gtgtcctttt tgcccgaatg ctggttcaaa atgccaaatt cctgctgctc
    14161 gacgaaccct tcaatgccgt tgatgcacgg acaacctacg agcttctcga cgtattgcag
    14221 aaatgccatt gcggcggaca cgccatcatc gccgtactgc acgattacga acaagtccgt
    14281 gcctactttc ccaataccct gctgctcgcc cgcgaaaaaa ttgcggcagg cgcaaccgag
    14341 accattctga cagaacccta cctcgcccaa gccaacgcca aaatgcagca acaggaaagc
    14401 cccgactggt gcgcctcata aatgccgtct gaaaccgaaa aaccatgaat ctctacgacc
    14461 tgctccttgc cccctttgca gaattcgact ttatgcgcta cgccctcgca tccgtcttct
    14521 gcctgtccct cagtgccgca cccgtcggcg tattcctcgt catgcgccgt atgagcctga
    14581 taggcgacgc attgagccac gccgtcctgc ccggtgccgc cgtcggctac atgtttgccg
    14641 gcttgagcct gcccgccatg ggtttgggcg gcgtagccgc aggcatgctg atggcactgc
    14701 ttgccggact cgtcagccgc ttcaccaccc tgaaagaaga tgccaacttt gccgcctttt
    14761 atctcagcag cctcgccatc ggcgtagtcc tcgtcagcaa aaacgggagc agcgtcgatt
    14821 tgctccacct ccttttcggc tctgtacttg ccgtcgatat tcctgccctg cagctcatcg
    14881 ccgccgtctc cagcctcacg ctcattaccc ttgccgtcat ctaccgcccg ctcgtactcg
    14941 aaagcatcga ccccctgttt ctcaaatccg tcggcggcaa aggcgggctt tggcacgtcc
    15001 tctttctcgt cctggtcgtc atgaacctcg tatccggctt tcaagccctc ggcacactca
    15061 tgtccgtcgg actcatgatg ctgccagcca ttaccgcccg cctgtgggcg aagcatatgg
    15121 gcgcactcat cctcctatcc gttctgacag ccctgctgtg cggcttgagc ggactgctca
    15181 tttcctacca catcgaaatt ccttccggtc ccgccatcat cctctgttgc agcgtccttt
    15241 atctcttttc cgtcatactc ggcaaagaag gcggcattct gaccaaatgg ctcaaaaacc
    15301 accgccacca caccacctga ccccgcaagc cgcttgaaca aacaggcggc ttgttttacc
    15361 ataacagatg ttacgttata tcacaggaga acagttatga aacacctcaa actcaccctt
    15421 attgccgcat tgctgaccgc ctccgcaact gccgcccccc tgccggttgt aaccagcttc
    15481 agcattttag gcgacgtagc caaacaaatc ggcggagagc gcgtatccat acaaagtttg
    15541 gtcggagcca accaagatac gcacgcctat catatgacca gtggcgacat taaaaaaatc
    15601 cgcagtgcaa aactcgtcct gctcaacggc ttaggacttg aagctgccga tgtgcaacgc
    15661 gccgtcaaac aaagcaaagt atcctatacc gaagcgacca aaggcatcca acccctcaaa
    15721 gccgaagaag aaggcggaca ccatcacgac cacgatcatg accacgaagg acaccaccat
    15781 gaccacggcg aatatgaccc gcacgtctgg aacgaccccg tccttatgtc cgcctatgcc
    15841 caaaacgttg ccgaagccct gataaaggcc gatcccgaag gcaaagttta ttatcaacaa
    15901 cgcttgggca actaccaaat gcagctcaaa aaactgcaca gcgacgcaca agccgcattt
    15961 aatgccgtcc ctgctgccaa acgcaaagtc ctgaccgggc acgatgcctt ttcctatatg
    16021 ggcaaacgtt accatatcga attcatcgcc ccgcaaggcg tgagcagcga agccgagcct
    16081 tcggccaaac aagtcgccgc catcatccga caaatcaaac gcgaaggcat caaagccgtc
    16141 tttaccgaaa acatcaagga cacccgtatg gttgaccgta tcgccaaaga aaccggtgtc
    16201 aacgtcagcg gcaaactgta ttccgacgca ctcggcaacg cgcccgcaga cacctacatc
    16261 ggaatgtacc gccacaacat caaagccttg accaacgcga tgaagcaata aaaccgtttg
    16321 attggtttga atttgaaaag gcaaaaggcc gtctgaaaat tatagtg
//
LOCUS       DAWIVB010000029        16174 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWIVB010000029 DAWIVB010000000
VERSION     DAWIVB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16174
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.029"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(46..1227)
                     /gene="odhB"
                     /locus_tag="WIX60_000672"
     CDS             complement(46..1227)
                     /gene="odhB"
                     /locus_tag="WIX60_000672"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ5304972.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAATKPAAAAAPTVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1327..4155)
                     /locus_tag="WIX60_000673"
     CDS             complement(1327..4155)
                     /locus_tag="WIX60_000673"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ5304973.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDAAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEGQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4360..5643)
                     /gene="gltA"
                     /locus_tag="WIX60_000674"
     CDS             complement(4360..5643)
                     /gene="gltA"
                     /locus_tag="WIX60_000674"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ5304974.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5755..6003)
                     /locus_tag="WIX60_000675"
     CDS             complement(5755..6003)
                     /locus_tag="WIX60_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ5304975.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6007..6714)
                     /locus_tag="WIX60_000676"
     CDS             complement(6007..6714)
                     /locus_tag="WIX60_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ5304976.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WIX60_000677"
     CDS             complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WIX60_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ5304977.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYQGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDINTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WIX60_000678"
     CDS             complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WIX60_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ5304978.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WIX60_000679"
     CDS             complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WIX60_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ5304979.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9591..10994)
                     /locus_tag="WIX60_000680"
     CDS             complement(9591..10994)
                     /locus_tag="WIX60_000680"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ5304980.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDDHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEAKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11243..11980)
                     /locus_tag="WIX60_000681"
     CDS             complement(11243..11980)
                     /locus_tag="WIX60_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ5304981.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWSAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12259..14535)
                     /gene="metE"
                     /locus_tag="WIX60_000682"
     CDS             complement(12259..14535)
                     /gene="metE"
                     /locus_tag="WIX60_000682"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ5304982.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGTVEFNRLSLLQKLLPVYVEILNALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVNAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(14671..15549)
                     /gene="metF"
                     /locus_tag="WIX60_000683"
     CDS             complement(14671..15549)
                     /gene="metF"
                     /locus_tag="WIX60_000683"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ5304983.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15750..16025
                     /locus_tag="WIX60_000684"
     CDS             15750..16025
                     /locus_tag="WIX60_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ5304984.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16025..16150
                     /gene="ykgO"
                     /locus_tag="WIX60_000685"
     CDS             16025..16150
                     /gene="ykgO"
                     /locus_tag="WIX60_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ5304985.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
BASE COUNT         3941 a         4090 c         4036 g         4107 t
ORIGIN      
        1 ttcagacggc cttttcatta acaaataaaa ggccgtctga aacgattaca gatccaacaa
       61 caggcgggct gggtcttcca acgcatcttt aatggctacc aaggtcaata cagcttcgcg
      121 gccatcgatg atacggtggt cgtaagacag agccagatac atcattggac ggacaacaac
      181 ttgaccgttt tcaaccacag cgcgctcttt agtggcgtgc atacccaaaa tcgcagattg
      241 aggcgggttg atgatcgggg tagacatcat agaaccgaaa gtaccgccgt tggtaatact
      301 gaatgtaccg ccggtcagat cttcgatagc aattttgccg tctttggctt ttttcgcgta
      361 atcaacaatt gcttgttcga tgtcggcaat gctcatttgg tcggcatcgc gcaggattgg
      421 tacaaccaaa ccgcgtgggc tgccaatcgc gataccgatg tcgaagtagc cgtggtacac
      481 aatgtctttg ccgtcaacag aagcattcac aaccgggtat tttttcaggg ctgcaacagc
      541 ggctttaacg aagaaggaca taaagcccag tttcacgccg tgttctttct cgaatttttc
      601 tttgtacttc gcacgcaagt ccatgattgg tttcatgttg acttcgttga atgtagtcag
      661 aatggcgttt tcttgttggg aagccaaaag gcgttctgca acacgggcgc gcaagcggct
      721 catcggtacg cgttcttcag gacgtgcgcc ggcaggaagt gcaacagtag gagcagcggc
      781 tgcggcaggt ttagtggcgg cattttgtac gtcttctttc aatacgcgac cgtcacggcc
      841 ggaaccttgc aatgcgttca cgtcaacacc ggtctcggca gccagtttgg cggcggcagg
      901 catagcggcg ttgttttgtg cggcagcagg agcggcagcc ggggcggctt cggcaggcgc
      961 agctgcaggt gcttcagcgg cggcagtagc agctgtatcg atgcgtgcca aaacttggtc
     1021 ggcaacaacg gtttcaccgt cttgcgctac gatttcaacc aatacgccgg cttgtggaga
     1081 aggtacttcc aaaaccactt tgtcagtttc gatatcgatc aggatttcgt cacgggcaac
     1141 tgcttcgcca acttttttct tccattccaa gagcgtgcct tcagatacgc tttcagacag
     1201 cataggtact tttacatcaa taatcatttt gtgtctccaa tggccctttc agacggcctg
     1261 ttgtgtgttt ttaattctgc atatgggtgt tccgcctgag cagcctcaga cggaatgcta
     1321 cttggcttac aatgccaaag cgtcttcaac caattgtttc aattgagcaa tgtgtttgct
     1381 tgagtagccc actgcaggcg atgcgctgct tggacgaccg gcataagaca gtttttgccc
     1441 ttcgctgatg acgtcttcga tgcggtggcg gatttggtag aacgcgcctt ggtttttcgg
     1501 ctcttcttgc gcccaaacca cagattttgc gttcggatat ttcgccagtt cggctttaac
     1561 ctcgtcgtat gggaacggat acagctgctc aacgcggacg atggcaacat catcttccag
     1621 tttacgctcg gcacggccgg cttccaagtc atagtaaacc tgaccggcac acaataccac
     1681 gcgtttcacg ctgtcgttgc ttgcgcgttc ggcggcatca ccgataaccg gacggaaagt
     1741 cgaaccttcg gtgaagtttt ccagcgggct cattgcacct ttgaagcgca acaggcgttt
     1801 ggacatgaaa atcaccagcg gtttgcggta tgaacccaag acttggcgtt gcaagaggtg
     1861 gaacatttgc gacgcttcag acggcatgat gacttgcata ttgttctcag aacacagttg
     1921 caaccaacgt tccacgcgtg cagaagagtg ctcagggcct tgaccgtcgt agccgtgcgg
     1981 caggatggtg gtcagaccgc acaaacggcc ccatttggtt tcgcctgaag acaggaattg
     2041 gtcaatagtc acttgcgcgc cgttggcgaa gtcaccgaat tgagcttccc aaatggtcag
     2101 tttgtcaggt gcggagcagg caaagccgta ctcgaacgcc atcacggctt cttcgttcaa
     2161 aatggagtcg ataaccagga actcgcccat gccttcgccc atatggcgca gaggaacata
     2221 agtaccgtcg tcccattttt cgcgtttttg atcgtgcaat acggcgtggc ggtgcgagaa
     2281 cgtgccgcgt cccgagtcct cgccggaaat acgcacgccg tgacctttgg ttacgaggct
     2341 ggcgtatgcc aaggtttcgg ccatacccca atctatggcc tgtttgccgg atgccatggc
     2401 tttacgcgct tcaatcacac gttttgcagt cggatgcagg gcaaagcctt ccggtacggc
     2461 ggtaaacttc tcagtgagac gttcaatatc tgcggcaggc aaaccggttt cgatgtgttc
     2521 gcgccaatct ttgccttggt atttgctcca gtcgatttgc gtgcgttgga agttgctcaa
     2581 cgttgtttgc tcgacgtgct cgcctttgtc caaagcatca cggtaagctt ggatgtaacc
     2641 gtcagcctcg gcttgggtta ccacgccttc ggcaatcagt tgctcggtgt acaaagcacg
     2701 cgcaccgggg tgttgcgata cttttttgta catcatcggt tgggtcaagg tcggatcatc
     2761 gccctcgttg tgaccccatt tacggtagca gacaacgtca atcacgatgt ctttatggaa
     2821 ttttttgcgg taatccaaag cggcttggat agcaaagcaa acgcgttcgg gatcatcgcc
     2881 gttcacatgg ataaccgggg cggaaaccat ttttgcgata tcggtacagt gtacggttga
     2941 acgggtatcg cggatatcgg aagtggtaaa gccgatttgg ttgttgatga cgatatgaac
     3001 cgtaccgccg gtggtataac cgcgcgtttt agacaggttg aatgtcgctt ggttgactcc
     3061 cagaccgata aatgcggagt cgccgtgaat caataccggc aagactttgt cgcggccgtt
     3121 ttcgcccaaa cgtttttgtt tggcgcgcgc agaaccttcc accaccgggt tgacgatttc
     3181 caagtgtgac gggttgaacg ccaaagaaac gtgcatcggg ccgtgcggcg tggcaatatc
     3241 ggagctgaag cccatatggt atttcacgtc gccgctgggc agtttgattt cggcacgacc
     3301 ttcaaattcg gcaaacaaat cgccgggttt tttgcccaaa atgttcacca aaacattcag
     3361 acggccacgg tgcgccatac cgatgatgac ctcttccaca ccgtctttac cggcgttttg
     3421 aatcaggtag ttcaaaccgg caatcgcgct ttcgccgcct tcgacaccga aacgtttctg
     3481 accgacatat ttggtatgca gataacgttc caaagtctcg gcagcagtca tctctttcaa
     3541 gatacggcgt ttttgatcgg cattgtaatg cggtgtggac aatacgcttt caaaataatt
     3601 gcgtacccag cggcgctctt cggtattggg aatatagata tattccaatg cgatgtggcc
     3661 gcagtaggtt tgtttgaggt tgctgatgat ttgggacaaa ggcagtttgc cgcgattggc
     3721 aaaatcgccc tcgcccatat tgaattgaag cgccatatcg gcatctgaca gaccgtggaa
     3781 tttcggatcg agggcttcaa tatcgcgcgg ggggatacgt ttgagcggat caagttgggc
     3841 tgcacccacg ccttggatac gataggcgga aatcagccgt aaaacgctga cttgcttttt
     3901 cagcattgcc tcatccgcac cgcccgcaac ggcagatgca attttctttt tcgccaaagt
     3961 aacaaatgat tcgcgaatcg gtgtgtgtgc gacatcgaca gcaaccgtcc ccggctgttt
     4021 gctcaaatcg gtgaaatact gcttccattt ttcatcaacc gcatcggggt tttccaaaaa
     4081 agcctcgtac aattcctcaa tgtaaggtgc gtttgaaccg aacaggtaag agaaattgag
     4141 tttttcgtcc atcatggcgt gcgctctttt ctttaaaata taaacaaagg gtagaagtcc
     4201 gcgtcctgac ggctaaactg atgccgcctg aaaagacatt ttattctacc ctattttaaa
     4261 ccggattgct tgtcttgatt tcatccgact acaatcagac ggattcggga aacaatccgg
     4321 ttctctgata ttgcctgttt gacaatatat taatgtatgt tacctctcgc ctggcggcac
     4381 ataatcgcgg cgttccgaac cggtataaag ctggcgcggg cggccgattt tcagcgaagg
     4441 atcgctaatc atctcgtgcc agtgcgaaat ccagcccacg ctgcgcgaca gggcgaagat
     4501 gacggtaaac atttcggtcg ggatgcccag cgcggacagg acgatgccgg aatagaaatc
     4561 gacgtttgga tacagtttgc gttcgataaa gaacgggtct ttcagcgcaa tctgttccaa
     4621 ttccatcgcc agtttgaatt tcggactgtc ttccaagccc aattccttca aaacttcata
     4681 gcaggtttcg cgcataatgc tggcacgcgg atccatattg cggtacacgc ggtgaccgaa
     4741 gcccatcaga cggtatttgc gttgtttcac accttccatg tatgcggcaa cattagacac
     4801 atcgccgatt tcgtccaaca ttttcaacac ggcttcgttc gcaccgccgt gcgaagcacc
     4861 ccacaggcag gcgataccgg cagcaataca ggcaaacggg ttcgcacccg aagaccctgc
     4921 cagacggacg gttgaagttg aggcgttttg ctcgtggtcg gcatgcaaaa taaagatgcg
     4981 gtcgagcgcg cgtgccaaaa cgggattggg tttgtagtct tcacacggcg tggcgaacat
     5041 catatgaagg aagttttcgg aataagaaag attattcttc ggataattga acggcagacc
     5101 gtttgaatag cggtagcaca ttgccgcaat ggtcgggatt ttagaaatca ggcggtaaat
     5161 cgcgattttg cggtgttcgg gattgctaat gtccaagctg tcttggtaga acgcagacag
     5221 tgcgccgacc acgccgacca tcatcgccat cggatgcgcg tcgcggcgga acccccggaa
     5281 gaaccaagtc agctgttcat gcaccatcgt gtggcggcgg acggtattgt caaattctgc
     5341 cttttgctcg ggagtcggca gttcgccgta aatcaacagg tagcagactt ccaaataatc
     5401 ggacttttcg gccagctgct cgatggggta tccgcgataa taaagcaagc cttgatcgcc
     5461 gtcgatgtaa gtaattttag actcacagct tgcggttgaa acaaatccgg ggtcgaagga
     5521 aaacaaacct gtattttttg tcagcccccg aatgtcaacc acatcgtgcc cgatgctggc
     5581 ttccaatacc ggcagctcca aacctgcctg acccggtacg ttgagtttga ttgatttgga
     5641 catattattg ctcctttgca agttataacc cacgttactg catccgcagc acgttctttg
     5701 ccttcagacg gcttttgcat tttgaaatct gcctgcaggc agatttcaga tgcctcatgc
     5761 ccgtctgatt ttttcaagca tcgggataag gtgccctttg tccgtttccg aatgcccgtt
     5821 aatcaaggca agcaattctt gatcttgaaa ttcaaggatt tcggaaaact cggacagctc
     5881 tttatcgctc aaatgctcga attctttttc cataaacctg ccgaagatta aatctaattc
     5941 caacaatccc cggcgggttt gaaaacggat tttccgtttg gcaatatcgt caaaaaccat
     6001 catttcttaa acggcccgtt tcaacataat ctctttaatc ttaccgatgg ctcgggtcgg
     6061 attcaagtgt ttaggacata cgtctacgca gttcataatg gtgtggcaac ggaacaaacg
     6121 gtatgggtcg ttcagattat ccaaacgctc attagtgatg gtatcacggc tgtccgcaat
     6181 gaaacggtaa gcattcagca aaccggacgg accgacgaat ttatcagggt tccaccaaaa
     6241 tgacgggcag gcagtcgaac agcaggcgca caaaatacac tcgtacaaac cgtccaactc
     6301 tttacgctct tcctgagttt gcagacgctc tttgtccgca tcaatcggat tatcgttgac
     6361 aacataaggt ttgacggaat ggtattgttt gaagaactgg gtcatatcca caatcaggtc
     6421 gcggataaca ggcagacctg gcagaggacg gattttaact ggctgtttca agccacgcag
     6481 atcggtcaaa cacgccaagc cgtttttgcc gttgatgttc ataccgtccg atccgcaaat
     6541 gccttcgcgg caggagcggc ggaaagacaa ggtatcgtct tgtgctttca ggcgtaccaa
     6601 agcatccaaa agtttcacgt cggtcggttc caattccaac tcgtaacgct gcatataagg
     6661 cttggcatca acatccgggt tgtaacggta aatttcaaaa ctcatttttt ccatgagaag
     6721 tgttcctttt ctcatcaact gcccaatgat tttgtattat aaattcaagc agttcaatgt
     6781 gggtatggtt agatttcaga cgacctttga ggcgaagact gtctgaaaac gcatcaataa
     6841 acgcgcttgg ccggtttgat gtattccacg ctcaaaggct tggtgtgcac cggtttgtag
     6901 gacaaggtat tgatatctga atggtacagc gtatgtttca tccagttttc atcatcgcgc
     6961 tcaggatggt cgtctgaagc gtgcgcaccg cgtgattctt tacgtgcttc ggcagacacc
     7021 aaagtcgctt tcgccacttc aatcaggtta tccaattcca aagcctcgat acgcgcggta
     7081 ttccacactt tgctcttgtc tttgatttcg gtacgtttca cacgctcggc aatcgccatg
     7141 acttctcgaa cgcctttgct cagaatctca tcagtacgga acacgccggc gtgcagttgt
     7201 acagagcgtt gcagttcgcg acgcagcgca tcgacgtttt cgccatcagt ttgattgtcc
     7261 aaacgctcga tacgttggcg ggtcagctcg cccgcatccg caggcaaagg tttccagtcg
     7321 ctttgctctt tgatgaattt aatcatgctg tcgccggcag ctttaccgaa caccaccaag
     7381 tccagcagag agttcgtacc caaacggttc gcaccgtgta cggaagcaca ggcgcactca
     7441 cctgcggcat acaggccttt tacaggcact tcgtattcgt ctccttgcgg cacaacgact
     7501 tcgccttggt aattggtcgg aatgccgccc atcatatagt gggtagtcgg cacaacagga
     7561 atcgggtctt taatcggatc gataccggcg aactgaatgg aaatctcgcg gatgcccggc
     7621 agtttttcca taattttttc tgcgccgata tggtcgattt tcagtaagac atggtctttg
     7681 tttttaccgc agccgcgacc ttcgtagatt tccatcgcca tcgcgcggga aacaacgtcg
     7741 cgagaagcca agtcttttac ggtcggcgca tagcgctcca taaagcgttc gccgtcggcg
     7801 ttcaacaaaa taccgccttc accgcgtaca ccttcggtaa tcaacacgcc tgcacctgcc
     7861 acacctgtcg ggtggaattg ccagaactcc atatcttcca acggaatacc cgcacgcgcg
     7921 caaatgccca aaccatcgcc ggtattcata taggcattgg tagaagaagc ataaatacga
     7981 ccgccaccgc cggtagcaaa catcacagct ttagcgtgga aaatataaac ttcgccggtt
     8041 tccatttcca tggcggttac gccgacgaca tcgccgtttt catcacgaat caaatcttgt
     8101 gccgtccatt ccacaaagaa ttgcgtattg gcacggacgt tttgttggta caaagtatgc
     8161 agcatcgcat gacctgtacg gtcggcaacc gcacaggcgc gttctaccgc gcgtttaccg
     8221 tgttcggcag tatggccgcc gaaaggacgc tgataaattt taccgctttc cacacggtca
     8281 aaaggcatac ccatgtgttc caactcaatt acggcttcag gcgcggcgcg gcacataaac
     8341 tcaatcgcat cttggtcgcc caaccagtcg gaacctttca cggtatcgta catgtgccag
     8401 tcccaacggt cttcctgcac attacccaaa gaggcggaaa taccgccctg cgccgctacg
     8461 gtatgcgaac gggtcgggaa cactttagac aaaacggcac aattcagacc ggatttggat
     8521 aattggaggg ctgcgcgtaa acctgcacca ccaccgccga caatcacggc atcaaacttg
     8581 cgaacaggaa aacccatact tacccccaaa ttactttaat tgaatacacc aagcagccga
     8641 ccagccagac gatggtggca acctgcaaaa acaaacgcac gccgaagggt ttgatatagt
     8701 ccatccacaa atcgcggata cccacccaag cgtgcaagaa tacggcgatg aagctcactt
     8761 gggtaaatac ttttacccaa gtttgactaa aaaatgcctg ccatgccgaa tattctttag
     8821 gcagggaaaa tagaaccact aaaagtgcaa cggtataaat caacataata accgcagtcg
     8881 cacgttgcat cgcccaatcg cgcaaaccgt aatgggcacc ggtcaattta cgttctacca
     8941 taacaacgct cccaaaacga cagtcaaaac caatgcagaa gcaaatacgg ctttagcggt
     9001 attgcgcgca gtattcagct caaggccttt gtgcgcatcc aaaaataaaa agcggatacc
     9061 ggcgagagaa tggtgcagat aagcccacaa tacaccgatt aaaaccagct tgaccaaagg
     9121 atgggaaaca atggcacggt aagtttcaaa tgcagactct tgactcaggg taccggacag
     9181 gaaatacagc aggaaaggca gcataataaa cagcccgacc ccgctgattc ggtgaaggat
     9241 ggaaactatc ccgggtatcg gcagacggat gttcggcaaa tccaaataaa caggacgcgg
     9301 tttggcagac atagtcaatt cctctgtagt tatatatcga ctcaaaaacc gtaaacccat
     9361 tgctacactg ggttacataa acgttaattt acctgtttta tcccattttg aacagtgtat
     9421 tttaaaaaca aactgataga ttttgacaaa cacgcgtgga aacagcagat taaaagaaag
     9481 atttttagtt ttgccgcccc cttcccgaca cggtatcagc atccgacccc atccccaaaa
     9541 acaaaatgcc gtctgaaaaa atttcagacg gcattgtttt gccatatcgg tcaggctttc
     9601 aatttcgcat cggcatcgcg caacgcggta cgcaaacctt cctcgataac gggatggtag
     9661 aacggcatat ccagcatttg cggaacggtc atcttcattt gatgtgccca agccaacagg
     9721 tgcgccaaat gttcggcggc cggacctacg atttctgcgc cgatgaagcg gccggtggct
     9781 ttttcggcat acaggcgcat atggcctttg tttaccagca tcacgcggct gcggccttgg
     9841 tttttgaacg atacttcgcc gatgacaaat tcgtcggctt ggtattgcgc ggcaacctgc
     9901 gcgtatttca aaccgacaaa gccgatttgc ggactggtga acactacgcc gatggtgctg
     9961 cggcgcaaac cgccgccgat attcgggtag cggcctgcgt tatcgccggc aatcttgcct
    10021 tggtcggcgg cttcatgcag caggggcagt tggttggacg cgtcgcccgc aataaagata
    10081 tgcggaatac tggtctgcat agtcagcgga tcggcaacgg gtacgccgcg cgcatctttt
    10141 tcgatattga tgttttccaa accgatattg tcaacgttcg gacggcggcc tacggctgcc
    10201 agcatatatt cggcaacaaa tacgcctttt tcgccatcct gctcccaatg gacttctaca
    10261 ttgccgtctg cgtcgagttt ggcctcggtt ttagcatcca aatgcagttt caattcttca
    10321 ccgaacacgg ctttcgcctc gtctgaaacg acggggtcgg aaatgccgcc gatgatgcca
    10381 cccaaaccga aaatttcaac tttcacgccc aaacggtgca atgcctgacc cagttccaaa
    10441 ccgataacac ccggtccgaa cacggcaacg cgcttaggca gcgtatccca tgagaaaacg
    10501 tcatcgttga taatcaaacg attgcccaaa gactgccatt gcggcaggat gacgggacgc
    10561 gaaccggtag caatcacgaa actttttgcc gtaatttggg tatggtcgtc gatttggacg
    10621 gtatgctcgt cgataaactt agccgagccc atgatgcgct tgtcggcagg ccactcttcc
    10681 acatcagcaa cgacaaagcc gacaaaacgg tcgcgctcgg atttaacacg ccgcattact
    10741 tcttcaccgt tgacgacgat gctgtctttg tccaaatgca cgccgaacgg gtcggtatgc
    10801 agtgcgtggt ggcgcgcttc tgcggcggca atcaagagtt tggaaggcat acaacccacc
    10861 cgcgcgcagg tcgtgccgaa cacattgttt tcaatcaggt aaacattatc cgaatgtaaa
    10921 cgggcattgc gaaacgcacc cataccggca gtaccgccgc cgattacgac gacatccgct
    10981 tgaatttttt tcataatctt tgtccttccg ttttcgtctg aaacattgcc gacacagaac
    11041 atatcggttt cagacggcat ttaaatattt tcccaatcaa aaacatcatt cagataaata
    11101 aaaacaaaat ttcgattgag tgcaacaata tccaaattct cttataaata tagagttatt
    11161 cggagcggca gtgtagaacc gcccgaataa ctctatccgg tatatcagac agtttaatca
    11221 aaccgcctta agcaaacagc tatcagtttt tagccaagta agcttccaaa tcttcgctgc
    11281 cgccgatgta tttgccgccg atgaagactt gaggggcagt catcttgccg gtaatggcgc
    11341 gaacggaagt aacggttgca tctttgccca atacgatttc ttcgtaagac aaacctttgt
    11401 cttgcaaagc ttgtttggct ttagcgcaga attggcaacc tggtttagtg aaaattgcca
    11461 cagactcttg agccttccaa tcgggagcaa cgaattgcag catagtatct gcatcggata
    11521 ctttgaaagg atcgcccggt tcttcaggtt cgatgaacat tttttcaacc acgccgtcgt
    11581 taaccagcat ggagtaacgc caagagcgtt taccgaagcc caagtcttct ttaccgacca
    11641 gcatacccat accttcggta aattcgccgt tgccgtcagg aatcatgtag atgttgtcgg
    11701 attcttcttc ggcagaccaa gcgttcatta cgaacgtatc gtttacagat acgcagcaga
    11761 ttgcgtcaac gccgttttct ttgaacgcgc cgaacaattc gttgtaacgc ggcaggtgtg
    11821 aagaagaaca agtcggggta aatgcaccgg gcagggagaa tacgactact tttttgcctt
    11881 tgaacaaatc atcggtagac acatctttcc aagtgtcgcc gacacgggtg cggaatacta
    11941 cggaaggtac tttttgaccg gtacgatctt gcaaagccat tttaagctcc ttaatagatt
    12001 ggtttgagaa ataattgtga atcgctgggc attctacccc atgcaaaatg atttgtataa
    12061 tttatagttc aaataatata aattatttaa aactatcaaa cacaactccg atttgcctgt
    12121 ttcccacata taaagccgaa ttagaaaaaa acaagctgca atgaaaagat tttccggcaa
    12181 ctgcgccctt gcgccgcaaa tcaggacaaa ataccgtctg aaaggctttc agacggtatt
    12241 tattcatacg gtctcggctt atttcgccaa ttcggcacgc aatttgtggg ttacgttcat
    12301 catcacttgg agttgttcca gagtttcttt ccagccgcgt gttttcaggc cgcagtccgg
    12361 gttaacccac agacgttcaa ccggtacaac ctcgatggct ttgcgcaaca ggtgctccac
    12421 ttcggcttct gtcggtacgc gcgggctgtg gatgtcgtaa acccccgggc cgatgtcgtt
    12481 cgggtatttg aattcgccga acgcggtcaa gagttccatg tcggaacgtg aagtctcgat
    12541 ggtgatcacg tccgcatcca ttgcagcaat cgcaggcagg atatcgttga actcggagta
    12601 gcacatatga gtgtggattt gtgtgctgtc ttcgcaaccg gcagaggaca ggcggaagga
    12661 ttcgcccgcc cagttcaggt aggcatccca atcggcgcgt ttcagcggca agccttcgcg
    12721 gatggcaggt tcgtcaattt ggatgacttt gatgcctgct ttttccagat ccaatacttc
    12781 gtcgttcaga gccagtgcga tttgtttgca cacggtagag cgaggaatat cgttgcggac
    12841 aaaagaccat tgcagaatgg ttacagggcc ggtcaacata cctttcatcg ggcgtttggt
    12901 caggctttgt gcgtaagtag accaggcgac agtcatggct tcaggacggc ttacgtcacc
    12961 gaagataatc ggtggtttaa cacaacgtga gccgtagctt tgtacccagc cgtattgggt
    13021 gaatgcaaaa ccgctcaaca attcgccgaa gtattcgacc atgtcgttac gctcggcttc
    13081 gccgtgtacc agtacgtcca aatccagttt ttcttgttct tcaaccacca aggcgatttc
    13141 ttttttcatc gcggcttcgt aatcggcggc agacagttcg ccttttttga aggctgcgcg
    13201 tgcttggcgg atttcggtag tttgcgggaa agaaccgatg ttggtcgtcg gcagcagagg
    13261 caggttcaac catgcttgtt gcgctttgat acggtcggca aatggagatt tgcgttggtc
    13321 tgcgttggca ggcaaatcgg ccaggcgttt ggcaacgtct gcacggtgga tttcgctgct
    13381 gttggcgcgt gaatcggcag cagcttggct ggcggccagt tcttcggcaa cggaatcgcg
    13441 accttcgttc aatgcggctt tcagaacgcg caattcttgg gttttttgca gggtaaatgc
    13501 caaccaagag tacaggtcgg gtttgttggc tttcagtttt tcttcaactg acaagtcaaa
    13561 tggagtgtgc agcagagagc aagagctgga gatccacaaa cggtcgccca gtttggcttg
    13621 cagaggctca acagtttcca aaactttgtt caggttggcg cgccaaatat tgcggccgtc
    13681 aatcacgccg gcggacagga ctttgtcgta gtcggcgaac gcgtccagtt gctcgggagc
    13741 gcgtaccaag tcgatgtgca gaccgtcaac aggcagggct ttcagcaatg cggcgtgttc
    13801 ggcaacagaa ccgaagtaag tgctcaacag gattttggcg ttgactttgc tcaaagtagc
    13861 gtaaacgtct ttgtaggctt ccacccattc tttaggcagg tcaacagcca aagcaggctc
    13921 gtcgatttga atccattctg caccggcttc aaccaaagcg tttaggattt caacgtaaac
    13981 aggcagcagt ttttgcaaca ggctcaggcg gttgaattcg actgtgcctt tttctttacc
    14041 cacccacagg aaagtcaacg gacctacaac ggtcggtttg gctttcagac cgagggcttg
    14101 ggcttcttgc agttgttgaa catagtgttt ggcattggct ttgaattcgg tatcggcgtg
    14161 gaattcaggc accaagtagt ggtagttggt gtcgaaccat ttggtcattt cgatagcgaa
    14221 ttggtctttg ttgccgcgcg ccagttggaa gaattgttcc aaagacaggt tttggctgtc
    14281 gaagccgaag cgggcgggaa tcgcgccggt ggcgacttgc aggtcgagga tgtggtcgta
    14341 gaaagtgaaa tcacctacgg caacgaaatc ggcgttggca gcggcctggt gtttccagtt
    14401 tttctcgcgc aagtctttag caacagccag caattcttgc tcgctgattt ctttgcgcca
    14461 gtatttttct tgtgcgaatt tcaattcgcg gaaggcgccg acacgcggga agcctgagaa
    14521 atgtaatgtt gtcatgttga actctccagt tggattttgt aaggaccggt tcgatccgaa
    14581 tcggtcggta ttgtaaatca gaatggctcg gatggggcgg cgttttgctt ttcagacggc
    14641 ctcgtatgcc gtctgaagcc gttttcggct ttaagggcgc acgcctaaaa tatggcaaat
    14701 ggcgtaagtc agctcgctgc ggttaagcgt atagaagtgg aaatctttca cgccttcgcg
    14761 ggacaggact ttgaccatat cgatggcgat acttgccgcc accagattgc gcgtgccttg
    14821 gtcgtcgtcc aaaccttcat acatttgcga cagccagctt gggattttga cgttggttac
    14881 ctgcgccatt ttgccgagct gcttgaagtt ggtaacgggc aggatgccgg ggacgatttc
    14941 cacatcgatg cccaacatca cgcagcggtc gcggaagcgc aggtagcgtt ccacatcgaa
    15001 gaagaattgg gtgatgacgt ggttcgcgcc cgcatcgatt ttgcgtttca aattaatcag
    15061 gtcggcttgt gcggatttcg cttcgggatg cacttcggga tatgccgcta cagaaatgtc
    15121 gaagtcggcg acggagcgta atagcttgac caagtcttcg gcgtaaaacg gttttttctc
    15181 gtaaccgggc ggctcgtcgc cacgcagggc gacaatgcgg cggatgccgc tgtcccaata
    15241 atctttggca atttggcgca attcgtcggg agaagcgtcg ataccggtca ggtgaggcgc
    15301 ggcttccaag ccggtttcct gtttgatgcg tttgacgatg ccgtgcgtgc ggtcgcgttc
    15361 gccggaattt gcgccgtaag ttacggatac gaacttggga tgcagggttt gcagacggtg
    15421 gatggagtcc cacagcatcg tttccatttg ttcgtttttc ggcgggaaaa attcaaacga
    15481 aacgttaatg tcgcctttca aatcagaaag actgttattt aacgcattga tttcttttgc
    15541 ataattcatg gctgcgtacc ttttattatc ttttatatga tattttcagg caacataata
    15601 aagttacgcc aaacatgagt caatttcaaa attttcagat ttatcatgaa aatttctaat
    15661 atattacaaa aatagttaaa tttccttgta caagccttct aatttatgat aaagtttgtt
    15721 atgttataac aattcaacta aggcgatata tgaaaccgaa tattcatcct gacaattatc
    15781 gaaccgtcct ttttttcgac agcagcgcca atgaaggctg gttaatccgc tcctgtgccg
    15841 aaacacacgg aaaaacgatg gtttggacag acggcaaaga atatccgctg ttttcgctgg
    15901 atacttcttc cgcatcccat cccgtctata ctggcaaaca gcgcaacgtc aacaccgaag
    15961 gccgtgccag caaattcaac caacgtttcc aatccgtcat gtcatcgttt agaaaggaca
    16021 aataatgcag gttctgtctt cactcaaaac cgccaaacaa cgccaccgcg actgccaaat
    16081 cgtcagacgc aggggcaaag tttacgtcat ctgcaaaagc aatccgcgtt ttaaagcacg
    16141 ccaacgctga ccccctgccc ttcctgcttc ggtt
//
LOCUS       DAWIVB010000030        15414 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWIVB010000030 DAWIVB010000000
VERSION     DAWIVB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15414
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.030"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(136..1920)
                     /gene="lpdA"
                     /locus_tag="WIX60_000686"
     CDS             complement(136..1920)
                     /gene="lpdA"
                     /locus_tag="WIX60_000686"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ5304986.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1938..2375)
                     /locus_tag="WIX60_000687"
     CDS             complement(1938..2375)
                     /locus_tag="WIX60_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5304987.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2469..4076)
                     /gene="aceF"
                     /locus_tag="WIX60_000688"
     CDS             complement(2469..4076)
                     /gene="aceF"
                     /locus_tag="WIX60_000688"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ5304988.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPAA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGDDIKAFVKSVMQ
                     GGAAKPAAASASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4226..6889)
                     /gene="aceE"
                     /locus_tag="WIX60_000689"
     CDS             complement(4226..6889)
                     /gene="aceE"
                     /locus_tag="WIX60_000689"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ5304989.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKTLVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7583..9295
                     /locus_tag="WIX60_000690"
     CDS             7583..9295
                     /locus_tag="WIX60_000690"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ5304990.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            9358..10014
                     /locus_tag="WIX60_000691"
     CDS             9358..10014
                     /locus_tag="WIX60_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ5304991.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(10024..10479)
                     /gene="ruvX"
                     /locus_tag="WIX60_000692"
     CDS             complement(10024..10479)
                     /gene="ruvX"
                     /locus_tag="WIX60_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ5304992.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10472..11020)
                     /locus_tag="WIX60_000693"
     CDS             complement(10472..11020)
                     /locus_tag="WIX60_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ5304993.1"
                     /translation="MNLSNHFLIAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(11022..11582)
                     /locus_tag="WIX60_000694"
     CDS             complement(11022..11582)
                     /locus_tag="WIX60_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ5304994.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENLDKR"
     gene            11910..13715
                     /locus_tag="WIX60_000695"
     CDS             11910..13715
                     /locus_tag="WIX60_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ5304995.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13835..15319
                     /locus_tag="WIX60_000696"
     CDS             13835..15319
                     /locus_tag="WIX60_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ5304996.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAVPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3698 a         3792 c         4255 g         3669 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata ggtcggagaa gatattttaa tccgctgtaa
       61 aatagaaaat gccgtctgaa catttcagac ggcatttgct caaataaacc ttatcggctg
      121 tttattcagt cggatttatt ttttcttttg cggaggcagg tcggtacaag tacccaatgc
      181 cacttccgcc gccataccga tggattcgcc caaggtcggg tgcgggtgga tggttttgcc
      241 gatgtctgcc gcgtcgcagc ccatttcgat ggcaaggcag acttcgccga tcatatcgcc
      301 accgttcgga ccgacaatgc cgccgccgat gatgcggccg gtttcggcat caaaaatcag
      361 cttggtaaag ccgttgtcgc aaccgttggc aatcgcacgg ccggaagccg cccacgggaa
      421 gttggctttg gtgattttgc ggccggaggc tttggcggac agttcggttt cgcccaccca
      481 cgccacttcg ggggaagtgt aggcaacgcc cggaatcacg cgcgcgtcga agtaggcttt
      541 gtggccggcg cagttttcgg cggcaacgtg gccttcgtga acggctttgt gcgccaacat
      601 cggctgaccg acgatgtcgc cgatggcgta gatgtgcggc acattggtac gcatttgttt
      661 gtccacttcg atgaagccgc gatcggttac ggcaacgcct gctttttccg cgctgatgag
      721 tttgccgttg ggcgcgcggc cggcggcaac caatacggca tcgtagcgtt gcggctcttt
      781 aggcgcgttc gcgccttcaa aggtaacgta aacgccgtct tctttcggct caactgcaac
      841 ggttttggtg ttgaccataa tgttgtcaaa acggtattcg ttttgttttt gccatacttt
      901 taccaaatcg cggtctgcgc cttgcatcag gccgtccatc atttcaacca catccaaacg
      961 cgaacccagc gtgctgtaaa ccgtacccat ctcgaggccg ataatgccgc cgccgataat
     1021 cagcagtttg cccggtactt ctttcagagc caatgcgccg ctggaatcga tgatgcgcgg
     1081 atcttcagga atgaaaggca gtttggttac gcggctgcct gctgcaatga tacagttttt
     1141 gaaggcaacg atttttttct cgccggtagg ggctgcctgt tcgtacgcgt cgccggcagt
     1201 cagcgacact tccaagtggt gcggatctaa gaattgcccg tcgccttgga taacgtccac
     1261 tttacggctt ttcgccatac ctgccaaacc gcccgtgagg cgggaaacta cgccgtcttt
     1321 gtaggcgcga agcatatcga tgtcgagttc cggctcgggg tatttgatac cgttggcagc
     1381 caagtggcgc acttcgtcga taacggcggc attgtgcaac aaggctttgg aagggataca
     1441 gccgacgttc aggcaaacgc cgcccaaagt tttgtaacgt tcgacgatgg cgactttcaa
     1501 gccttcatcg gcagcggcaa atgcagcgga gtaaccgccg ggaccgccac ccaatacgac
     1561 cacgtcgtac tcggcatcgg cagaaccgcc gaattgcgcg gcttgcggag caggagcggc
     1621 agctttaggg gcttcttgcg ccggggcggc agccgcttcg gctttaggag cggctgccgt
     1681 gccttcagct tcaacgacga caatcaaacc accttcagag attttgtcgc cgactttaac
     1741 tttaacttct ttgactacgc ctgcaacttc agcaggtacg tccatagtcg ctttatcggt
     1801 ttccaaagta atcagggtat cgtccacagc aatagtgtcg cccacgttta cttcaaccgc
     1861 gataatatct acattttcgt gtccgccaat gtcgggcact ttcaattcaa ctaaagccat
     1921 tatattgacc tttcaactta gttaatttct atcaactctt tagcaataat cacttcttca
     1981 ggaatacagc cacaatcttc agctctgatt gttacttcct tctcatttgg gttttctgga
     2041 tgttcaacct catattcaaa aatagaatat tgaccaaaca aatccctatt taaaacatat
     2101 atatagccat tttcgatgcc ggaacttgtt gcaaatttct tggcaatttc cttgtctgtt
     2161 gtcgtagata tataacatcc gtcatataga cctgtttcaa tttgatgggc gtaaactgca
     2221 ttcttcacac ttggaccatg tgtagcttta ccatcatatt taaacttacc atcataacga
     2281 attgcaactt cagctttatt acctttaggt tttaactgtc cattattttg ctcatcttgt
     2341 tggcaactaa tgcctctata taagaaattt cccataagtt attaatttaa aggttgaatt
     2401 taacaccatt actcatcaat ctcaagcaat ccaaacatta tcagactgct tgagagggat
     2461 gttttatttt ataaggtaat gcggcggaag tctttcaaca gcttcgccaa gaatacggtg
     2521 aagcgcatac cggccgcacc gtcgatgaca cggtggtcga aggacaggct caacgggcac
     2581 atcaggcgcg gggcaaactc tttgccgttc caaacaggtt tgatttggga tttgcacacg
     2641 cccaagatgg cgacttcggg agcgttcaca attggcgtga agcctgtgcc gccgatgccg
     2701 cctaagctgg aaatggtaaa gcacgcgcct tgcatttctt gcggtttgag cttgccttca
     2761 cgggcttttt tggacaattc ggtcaattct tggctgattt gtttcaagcc tttttgatcc
     2821 acgtctttga tgacgggaac aaccaagccg ttcggcgtat cggctgcgaa accgatgttg
     2881 aagtagtttt tcagcaccag gttgtcgccg tccagtgagg cgttgaattc ggggaatgct
     2941 ttcaacgcgg aaacagaggc tttgatgatg aacgccaacg gggacagttt cacgccttcg
     3001 cgttcccatt ctttgttcag ctgtttgcgg aattcttcca gctcggtcat gtccgcttct
     3061 tcgtgtacgg taacgtgggg aatcacaacc cagttgcggg acaggttttg accggaaatt
     3121 ttcttaatgc gggacaattc tttaacttcg acattgccga atttggagaa gtccacttta
     3181 ggccacggca gtaagtccag accgccgccc aaagatgcgc tggctgcggc aggttttgcc
     3241 gcgccgccct gcatcacgga tttcacaaag gctttgatgt cgtcgcccat gatacggcct
     3301 ttcaagccgg tgcctttgac ttggcccaaa tccacgccca attcgcgcgc cagtttgcgt
     3361 gcggaaggac cggcgtgtgc tttggcgaaa gcggcctcgt cgattttggc agcggcaggt
     3421 gcagaaggtg caggcgcggc ggcgggtgcg gcggcagcag tcggaggcgg agcggcagcc
     3481 ggtgcggcag cttgagcggc ttgagcaggg gctgctgcgg cagagccgac ggtttctact
     3541 tcgataatgg cagagccttc ggatactttg tcgccgactt ttaagaatac ggctttaacg
     3601 acaccggcag cggtacaagg tacgtccatt gtcgctttat cggtttccaa agtaatcagc
     3661 gtgtcgtctt cggcaacggt gtcgcccact ttgatttcaa cggcgattac atccacatcg
     3721 gtatggccgc cgatatcggg aacggctact tgaacggttg caccgcctgc ggcagcgggt
     3781 gcggcagcag gtgcaggttg tgcttcggca gcagccgggg cggcttcggc ggcggcggca
     3841 ccggtttcaa cggtcagaat tacgccgcct tcggagattt tgtcgccgac tttgactttt
     3901 acttctttca cgacaccggc cgcatcggca ggcacatcca tcgtggcttt gtcggtttcc
     3961 agtgtaatca gggtgtcgtc aacggcgatg gtgtcgcccg ctttaacttc tacggcgatg
     4021 atgtcgacgt tttcgtgacc gccgatatcg gggactttga tttctacgat actcatttga
     4081 gttcctttaa tctttttcaa ttggatgccg tctgaaagct gcttcaggcg gcatcggtac
     4141 ggtttgtctg ctcaaaccat tcgggcattc ggttttcaga cggcctgatg tcggaatggg
     4201 gcaaacaggc cgtctgaaac atcaatcagc gtttccagct aggagctgaa tcggctttga
     4261 tgccgtattt ctcaatggct tgttgaacgg tttctttgct gactttgcct tgttccgcca
     4321 atgcggccaa tgcggccacg gcaacgttgt agcgatccac ttcaaagaag cggcgcaggt
     4381 tggcgcgact gtcggaacgg ccgaaaccgt cagtgcccaa gacgtggtag tcgttcggga
     4441 tgtaggcgcg gatacggtca gcatagctgc ggatatagtc ggtagcggca ataaccggac
     4501 cgtcatgacc ttgcagttgg gaagtaacga aaggtacttt ttcggcttcc agcggatgca
     4561 ggcggttgaa gcgttctacc tcgacagcgt cgcggtgcag caggttgaag gacgggcaag
     4621 accagatgtc tgcttctacg ccgaagtcgg ctttcagcag ctcggcaccg gcaatgactt
     4681 cttgcaggat ggtaccggag cccatcaatt gaactttctt atcgcctttg ccgccggctt
     4741 tcagcaggta catacctttc aagatgtctt gttccgcacc ttcgggcata tccggatggg
     4801 tgtagttctc gttcatcagg gtgatgtagt agaacacgtc ttcattattg gcatacatac
     4861 ggcgcagacc gtcttgtacg atgacggcga cttcgtattg gaaagtcggg tcataagata
     4921 cgcagttcgg aatcaggtcg gcctggatgt ggctgtggcc gtcttcgtgt tgcaggcctt
     4981 cgccgttcag cgtcgtacgg ccggcagtac cgcccagcag gaagccgcgc gcgtgcatat
     5041 cgcccgccgc ccaagccaag tcgccgatac gttggaaacc gaacatagaa tagtaaatgt
     5101 agaacggaat catggcgaag ttgctgttgg cgtagctggt tgcagccgca atccagtcgg
     5161 ccatcgcgcc cggttcgtta ataccttctt gcaagatttg accgtcaacg gattctttat
     5221 agaacatcag ttggtctttg tcttgagggg tatattgctg acctttcgga ttccaaatac
     5281 cgtattggcg gaacatacct tccatgccga aagtacggct ttcgtcggga acgataggta
     5341 cgacgcgttt gccgattttt ttgtctttca gcaaagtgga caggatgcgg acgaatgcca
     5401 tcgtggtcga gaactcgcgt tcaccgctgg atttgagttg tgcgtcgaat gctgacagct
     5461 cgggcacttc caatacttcc tgcgtcggtt tgcgttgcgg caggtagcca cccaaagcat
     5521 cgcggcgtgc gtgcaggtat ttgtattctt ccgtatcggg ggcaaaagtc aggtaaggca
     5581 gatcgccgct ttcgatttgt tcgtcggtaa ccggaatgtc aaagcggtcg cggaattgtt
     5641 tcagggacgc tttgtccatt tttttggctt ggtgggcaac gttctgacct tcgccggatg
     5701 cgcccatacc gtaaccttta atggttttcg ccaagatgac ggtaggtttg ccgtcggcat
     5761 ggttcgctgc gcggtcgtag gcgttgtaca ctttttgcgg gtcgtggccg ccgcggttca
     5821 atgcccagag ttgctcatcg gtcatatcgg caaccaatgt tttcagttcg ggcgtattga
     5881 agaagtgttc gcgcacatac gcgccgtctt tggatttgta agtttggtag tcgccgtcca
     5941 aacattcttc catacgttgg cgcaggatac cgtctttgtc tttcgccaag aggcggtccc
     6001 agcggcggcc ccaaatgact ttgacgacat tccagccggc gccggcaaag ttgccttcca
     6061 attcttggat gattttgccg ttgccgcgta ccggaccgtc caagcgttgc agattgcagt
     6121 tgatgacgaa aatcaggttg tccaagcctt cgcgtgcagc cagtgcgatt gcaccttgag
     6181 attcgggttc gtccatttcg ccgtcgccgc agaaacacca tactttacgg cctttggttt
     6241 ttgccaaacc acgcgattcc aagtatttca ggaaacgcgc ctgataaatc gccatgatgg
     6301 gccccaagcc catggatacg gtcgggaact gccaaaagtc gggcaagagg tgggggtgcg
     6361 gataggaagg cagaccgtgt ccgtccactt cttggcggaa gttgttcagc tggtcttcgg
     6421 tcagacggcc ctcgacgaat gcgcgtgcat agatgcccgg ggcgacgtga ccttggaaga
     6481 agaccaaatc gccttcttcg ccttcgcctt tggctttcca aaagtggttg aaaccgactt
     6541 cgtacatggt ggcggcagat tggaaagatg cgatgtgccc acccagttcc aaatctttct
     6601 tgccggcgcg caatacgatg gcggcggcgt tccagcgcac gaatgcgcgg atgcggtgtt
     6661 cgatgttttg gtcgcccgga atgccttttt cgttttcaac cgaaacggta ttcaaatacg
     6721 gtgtggtcgt gccgtgtggc atacgtacgc ccttgtcgcg gcagtatttg accaggtttt
     6781 ccaagaggta ttgcgcgcgt tcgccgcctt catattcgag gacggagctt aacgcgtcca
     6841 gccactcttg ggtttcgata gggtcaacat cgtgtaattg ggtggacata gtatctatcc
     6901 tttatgttga gtgtttaatc ggcagggagg gtgtagccaa cccctttatt ttcgtttgtg
     6961 aaaaataata agcaaaaacg aaaatttcta aatcaaaccg aaaaaaataa tttaagttta
     7021 atgttttcaa aaaattataa aagttttgga aacaagggaa aacttgaaaa aaatgttcga
     7081 tctgctccac acgaaatcct atcagcaatc gggtctgctg gcaaataacc atgcctgtaa
     7141 aagcaggttt tatactgaag ttggcgtttg gtaaggtcaa gtctttttga tttcgaaata
     7201 ttctgtttga ttttggcgat attttttcgg aattgtggta tatcgaatca ttatgtctct
     7261 gtcggcaatt ttcctattgt agaaaatcta tacacaagct ttgtggataa ccgtgttgat
     7321 aagccacggc ttgtgttaaa aaagtcttac gcaacaaatg tacgcttatt ttgattaaaa
     7381 aataggcaca tttaaatcta taaaaatcat gtgcttatgt tcaatatgat gtttctggtc
     7441 atgtttttgc cgtctgttat gggatttctc ccgcgcttgg ggcagggggt gaattttgga
     7501 ggttggtgca aaacctgctt ttttcgcgtt acaatctgca cccgatttac ttttgaattt
     7561 gcctgtttgg aaaaatactt ttatgaaagc cagccaattc tttatctcta ctttaaaaga
     7621 agcccctgcc gaagccgcgc ttgccagcca caagctgatg attcgcgccg gtctgattaa
     7681 agccaacgcg tccggccttt atacttggat gccgatgggg ctgcgcgtgt tacgcaaagt
     7741 cgaaaacgtc gtgcgcgagg aaatggcgcg cgcgggcagc gtggagctgc tgatgccggt
     7801 ggtgcagcct gccgagctgt ggcaggaatc cggccgctgg gaattttacg gtaaagaact
     7861 gctgcgcctg aaagaccgcc acgaccgcga tttctgcatg ggcccgacct gcgaggaagt
     7921 catcgctgac atcgtgcgca aagaaatcaa cagctacaaa cagctgccga aaaactttta
     7981 ccacatccaa accaaattcc gcgacgaagt gcgcccgcgt ttcggcgtga tgcgcgcgcg
     8041 cgagttcgtc atgaaagacg cttattcctt ccatgccgac tacgcctcgc ttcagacgac
     8101 ctatgatgcc atgtatgact cttactgccg catcttcacc cgtctgggct tggcattccg
     8161 ccccgtcgcc gcagacaccg gcagcatcgg cggtacgggt tcgcacgagt ttcaagtgtt
     8221 ggcggaaagc ggcgaagatg ttattgcgta cagcgacact tccgattacg ccgccaatat
     8281 cgagttggca ccgaccttgc cgcttaaagg cgaacgcgcc gctgctcagg ctgtgttgac
     8341 caaagtacat acaccaaacg tcaaaaccat tgagtctttg gttgaattcc tgaatattcc
     8401 ggttgaacaa accctcaaat ccatcgtggt cgaaggcgaa aacgaaggcg aaatcgtctt
     8461 attactgttg cgcggcgacc atgagttcaa cgacatcaag gcagaaaaac tcgcaggcgt
     8521 gagatcgcca ttgacgatgg caagtcctgc cgcgattgtc gaacagttcg gcgcaaacgg
     8581 cggctcgctg ggtccggtca gctttgcagg taaagtttat gccgattttg ctactgaaaa
     8641 aggagcggac tgggtcatcg gtgcgaacga agacggctac cactataccg gcttcaactt
     8701 cgggcgcgat gccgccgagc ctgaattcgt cgatttgcgt aatgtggtcg aaggcgacga
     8761 aagccccgac gggcaaggcc gtctgaaact ggcgcgcggc atcgaagtcg gacacgtttt
     8821 ccaattgcgc gacaaataca cccaagccat gaacgtaagc ttccttgaca acaacggcaa
     8881 atcgcaaatt atggaaatgg gctgctacgg catcggcatc acccgcgtcg ttgccgccgc
     8941 catcgagcag aataacgacg aaaaaggcat catctggacc aaagcaatgg cgccgtttga
     9001 agtcgttatc gtgccgatga actacaaaaa atcagacacc gtacgcgaag ccgccgacaa
     9061 aatctatgcc gaattgctgg cggcaggcgc agacgtcctc ctcgacgacc gcgacgaacg
     9121 cgcaggcgtg ttgctgaacg attccgagct gttgggtatt ccgcaccgca tcgtcatcgg
     9181 cgaccgcgct ttgaaagaag gcaatgtcga atacgccgaa cgccgcaaca acgaagcgca
     9241 ggcagttgca atcggagaaa ttgttgcgcg tgtaacagct tcattaaatg ggtaagcttg
     9301 gtcaaatgct gtctgaaaat aatttcagac ggcattttta tttggggaga aagtgaagtg
     9361 gcttcagtat ttttagaagg agaggcagtc gaggtcggca atattttctg tatcggcagg
     9421 aactatgccg cacatatcga agagttaaaa aacgaaatac cttccgaacc tgtggtgttt
     9481 atgaagccgt caggcagcat tttgaacagc ggcggtacaa tcttgctgcc cgagtttagt
     9541 agggatgtgc agtttgagtg cgaactcgtg ctgctggtcg gtaaggattc agacggcacg
     9601 ggcgagggta aagatatttt gggatgtgtc gcaggatatg gtgtagggct ggaccttacg
     9661 gcacgggata tccaatgccg tctgaaggaa aaagggctgc cctggctgaa ggcaaaaggg
     9721 ttcaggcatt ccgcgtgcgt gtcggatttt gcggcggcag gcagaatcgg caatcccgag
     9781 aaggtgttgt tttcattaaa acaaaacggc gtattgaaac agcgcgggga taccggtttg
     9841 atgatttacc cgattcggga aattttacac aaactggcgg cagattacgg tttggggaaa
     9901 ggggatttgg tgtttaccgg aacgccctcc ggcgtgggcg caatcggtgc gggggacaat
     9961 ttggcattgg agctggacgg cttggtgcgc gcctctttca cggtagggtg ttaaccgcgc
    10021 cgcttaaccc tcacgcccgt tgaaacattc cgccggaccg ccctcgaaaa aaccgtgcaa
    10081 gatggcttgc gccgccactt ggtcgagcac cgatttgcgt tttttgccga agacctgtgc
    10141 ttccgaaagc aggctttcgg catagacgga cgacagccgt tcgtcaaccc aatagacggg
    10201 gagattgaac ctgccgttca gcctgcgtcc gaacttgcgc gacaggtgcg tcatttcatg
    10261 tttcgtgccg tcggtatgca cgggcagtcc gacgacaaaa taacgcggct gccattcttg
    10321 aaccagcttg gcgattgccg cgaacttttc atcgttgctg ccgccggtaa cggtgctcaa
    10381 aggatgggat agccctaatt ccgcgtctcc ttgtgccacg ccgatacgcg cttcgccgaa
    10441 gtcgaatgcc agtgccgttc cttttggaat tttatgcatg gccggctcct gaaaacaggg
    10501 cgagcgggtc gatgccgagt ttggcgaatg cggcggcgta acggtgttcg taggggatgt
    10561 cgaacaggat gtgttcgtcg gcgggaacag tcagccacgc attgtcggca agttcgcgtt
    10621 cgagctgccc tttgctccag cttgaatagc ctatgctgat caaggctttg tcaaccgcac
    10681 cttcgcgtga aatattttca atcacgtctc gggaagaagt tagcgcgata ttgtctgaaa
    10741 cgccgatact gctttgccag ttgccgatcg gggtatgcac gacataaccg cgctcgacct
    10801 gcaccggacc gcccatcatc acgctgtcgt gctgcatccg catggggatg tttttgccgg
    10861 tggcggaaaa aatcatgtcc atcgtaatcg gagagggttt gttgatggcg atgccgagtg
    10921 cgccgtcttc atcgtgtttg cagatataga cgaccgattg tgaaaaaaac gcgtcttcca
    10981 tatcgggcat ggcgatgaga aagtggttgg agaggttcat atcagcgttt gtcgagattt
    11041 tctatcgggt tggaaatcat gcaggcgccg gcaagcaggt tgccggaagt atcggcttgc
    11101 accccatcgg tttgcggtat gccgccgccg aaacaggaaa ccgcgcttaa ggaatttttc
    11161 ggcgagcctt ggatgatttt atcgctgtac accaaatagg cgaaggtttt gcgtttgggg
    11221 tcgtaataac ggacaatctg ccggctcttg aacgcgaagc tcgcaccgtg tttgaaaact
    11281 tctttcggtt tgcgcacggc ggtttcgtca aaagaaatcg aagatgccgt ctgaacgcac
    11341 gaaaccgatg cgtcggacgc gtcctcttcc aaattgacca tttccttcaa gccgcctttt
    11401 tttgcatacg aaatataaca ggcaacccct tgaacgtcgg gatcgtcgaa tccttccact
    11461 tcgatacggt cgtttttgcc cagtatgttg aaaacggtac ttgcccgtcc gattttatcg
    11521 gtttcgccgc tgccgcaggc agtcagcagg acggcggcag acagcagtag cagtctgttc
    11581 atcaaaattt ccttatcggt tgaatccccg ttttccgtga aagcggcagg gcttgctttg
    11641 tttggagggg cgcacgttcc caaaatcggg caacatacgg tatgcggctt aatgtgctac
    11701 cctgtgtgtc ggaacacaat atccgttgcg gcattgaaaa tatgacgcac aatataaggc
    11761 tgtttgagcc gattgcaagt atccgcgcaa agaaggcgca agattgggca aacggcattt
    11821 tcagacggca taactgacag tataatccaa acgtccggct gctttcgtgc agccttcagt
    11881 attatccaac tttttccgaa ggtcgaacca tgcgctacaa accccttctg cttgccctga
    11941 tgctcgtttt ttccacgccc gccgttgccg cccacgacgc ggcacacaac cgttccgccg
    12001 aagtgaaaaa acagacgaag aacaaaaaag aacagcccga agcggcggaa ggcaaaaaag
    12061 aaaaaggcaa aaatggcgca gtgaaagata aaaaaacagg cggcaaagag gcggcaaaag
    12121 agggcaaaga gtccaaaaaa accgccaaaa accgcaaaga agcagagaag gaggcgacat
    12181 ccaggcagtc tgcgcgcaaa ggacgcgaag gggataagaa atcgaaggcg gaacacaaaa
    12241 aggcacatgg caagcccgtg tccggatcca aagaaaaaaa cgcaaaaaca cagcctgaaa
    12301 acaaacaagg caaaaaagag gcaaaaggac agggcaatcc gcgcaagggc ggcaaggcgg
    12361 aaaaagacac tgtttctgca aataaaaaag tccgttccga caagaacggc aaagcagtga
    12421 aacaggacaa aaaatacagg gaagagaaaa atgccaaaac cgattccgac gaattgaaag
    12481 ccgccgttgc cgctgccacc aatgatgtcg aaaacaaaaa agccctgctc aaacaaagcg
    12541 aaggaatgct gcttcatgtc agcaattccc tcaaacagct tcaggaagag cgtatccgcc
    12601 aagagcgtat ccgccaagag cgtatccgtc aggcgcgcgg caaccttgct tccgtcaacc
    12661 gcaaacagcg cgaggcttgg gacaagttcc aaaaactcaa taccgagctg aaccgtttga
    12721 aaacggaagt cgccgctacg aaagcgcaga tttcccgttt cgtatcgggg aactataaaa
    12781 acagccagcc gaatgcggtt gccctgttcc tgaaaaacgc cgaaccgggt cagaaaaacc
    12841 gctttttgcg ttatacgcgt tatgtaaacg cctccaatcg ggaagttgtc aaggatttgg
    12901 aaaaacagca gaaggctttg gcggtacaag agcagaaaat caacaatgag cttgcccgtt
    12961 tgaagaaaat tcaggcaaac gtgcaatccc tgctgaaaaa acagggtgta accgatgcgg
    13021 cggaacagac ggaaagccgc agacagaatg ccaaaatcgc caaagatgcc cgaaaactgc
    13081 tggaacagaa agggaacgag cagcagctga acaagctctt gagcaatttg gagaagaaaa
    13141 aggccgaaca ccgcattcag gatgcggaag caaaaagaaa attggctgaa gccagactgg
    13201 cggcagccga aaaagccaga aaagaagcgg cgcagcagaa ggctgaagca cgacgtgcgg
    13261 aaatgtccaa cctgaccgcc gaagacagga acatccaagc gccttcggtt atgggtatcg
    13321 gcagtgccga cggtttcagc cgcatgcaag gacgtttgaa aaaaccggtt gacggtgtgc
    13381 cgaccggact tttcgggcag aaccggagcg gcggcgatgt ttggaaaggc gtgttctatt
    13441 ccactgcacc ggcaacggtt gaaagcattg cgccgggaac ggtaagctat gcggacgagt
    13501 tggacggcta cggcaaagtg gtcgtggtcg atcacggcga gaactacatc agcatctatg
    13561 ccggtttgag cgaaatttcc gtcggcaagg gttatatggt cgcggcagga agcaaaatcg
    13621 gctcgagcgg gtcgctgccg gacggggaag aggggcttta cctgcaaata cgttatcaag
    13681 gtcaggtatt gaacccttcg agctggatac gttgatcgga ttgccgaaac ggtaggcttt
    13741 atgcaaatcg tgccgatatg gtgcgctgtt ccgtgaagtt tgccgtacct gtattttccg
    13801 gtttttggaa atcaagagag aaagttaaag aagaatgtcg aaacctgttt ttaagaaaat
    13861 cgcactttat actttgggtg caatcagcgg cgtggccgtc agtctggcgg tgcagggttt
    13921 tgccgccgag aaggacaggc gggataacga agtcctgccg gtgcaatcca tccgcacaat
    13981 ggcggaggtt tacggtcaaa tcaaggcaaa ctactatcag gacaaacccg atgccgattt
    14041 gtttgaaggt gcgatgaagg gtatggtggc cggtttggat ccgcattccg aatatatgga
    14101 taaaaaaggt tatgccgaga taaaggagtc caccagcggc gaatttggcg gcttggggat
    14161 ggaaatcggg caggaagacg gatttgtcaa agtggtttcg ccgattgagg acacgcctgc
    14221 ggaacgggcg ggggtgaaaa gcggcgattt cattgtgaaa atcgataatg tttcgacacg
    14281 cggcatgacg gtcagcgaag cggtgaagaa aatgcggggc aagccgggta cgaagattac
    14341 tttgacgctg tcgcgcaaaa atgccgacaa gccgatagtc gtcaacctga cccgtgccat
    14401 tattaaagtg aaaagcgtcc gccatcacct gatcgaaccc gattacggct atatccgcgt
    14461 gtcgcagttc caagagcgga cggtcgaaag cgtcaatacc gccgcaaaag agctggtaaa
    14521 ggaaaataaa ggaaaaccgc tcaaggggct ggtgttggat ttgcgcgacg accccggcgg
    14581 gcttttgact ggcgcggtcg gcgtgtcggc ggcatttctg ccgtctgaag cagtcgtcgt
    14641 cagcaccaag ggacgcgacg gcaaagaccg catggtactg aaagccgttc ctgaagatta
    14701 tgtgtacggg atgggcggcg attcgttggc gggcattcct gccgagttga aaaccatacc
    14761 gatgacggta ttggtcaatt ccggttcggc ttccgcgtcg gagattgtcg caggtgcatt
    14821 gcaggatcat aaacgcgcgg tcatcgtcgg tacgcagagc ttcggcaaag gttcggttca
    14881 gactttgatt cctttgtcca acggcagcgc ggtcaagctg acaacggcac tgtattatac
    14941 gccgaacgac cgttctattc aggcgcaggg gattgttccc gatgtcgaag taaaagataa
    15001 ggaacgcatt tttgaaagcc gcgaggcgga tttggtcgga cacatcggca atcctttggg
    15061 cggcgaggat gtgaacagtg aaacccttgc cgtgccgctt gaaaaagatg cggataagcc
    15121 cgctgtaaaa gaaaaaggta aaaagaaaaa ggacgaggat ttgtcttcaa ggcggattcc
    15181 caaccctgcc aaagacgacc agttgcggaa agctttggat ttagtcaagt cgcccgagca
    15241 gtggcagaag tctttggggc tggcggcgaa aaagccggtt tcaaataaag ataagaaaga
    15301 taaaaaagat aagaagtagg ttttgacgat gccgtctgaa aggatttcag acggcatatt
    15361 atgtttgaaa ggacaggtta tgagcgattt tttaccggaa tttgaaccgt gcgg
//
LOCUS       DAWIVB010000031        15303 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWIVB010000031 DAWIVB010000000
VERSION     DAWIVB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.031"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..140)
                     /locus_tag="WIX60_000697"
     CDS             complement(<1..140)
                     /locus_tag="WIX60_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ5304997.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="WIX60_000698"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="WIX60_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ5304998.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ETVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="WIX60_000699"
     CDS             1619..2668
                     /locus_tag="WIX60_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ5304999.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /locus_tag="WIX60_000700"
     CDS             3356..4777
                     /locus_tag="WIX60_000700"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ5305000.1"
                     /translation="MTTIYNTLTRQKEPFSPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGKLTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGRLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4860..5639
                     /gene="xth"
                     /locus_tag="WIX60_000701"
     CDS             4860..5639
                     /gene="xth"
                     /locus_tag="WIX60_000701"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ5305001.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5626..5970
                     /locus_tag="WIX60_000702"
     CDS             5626..5970
                     /locus_tag="WIX60_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305002.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPMLVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5981..6511
                     /locus_tag="WIX60_000703"
     CDS             5981..6511
                     /locus_tag="WIX60_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ5305003.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHAFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6739..7854)
                     /gene="asd"
                     /locus_tag="WIX60_000704"
     CDS             complement(6739..7854)
                     /gene="asd"
                     /locus_tag="WIX60_000704"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ5305004.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRDVLDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADALADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            8030..8611
                     /locus_tag="WIX60_000705"
     CDS             8030..8611
                     /locus_tag="WIX60_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ5305005.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGSGEEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8673..9527)
                     /gene="folD"
                     /locus_tag="WIX60_000706"
     CDS             complement(8673..9527)
                     /gene="folD"
                     /locus_tag="WIX60_000706"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ5305006.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQNNLHTPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9750..9827
                     /locus_tag="WIX60_000707"
     tRNA            9750..9827
                     /locus_tag="WIX60_000707"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9785..9787,aa:Pro,seq:tgg)
     gene            9852..9928
                     /locus_tag="WIX60_000708"
     tRNA            9852..9928
                     /locus_tag="WIX60_000708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9886..9888,aa:Arg,seq:tct)
     gene            9956..10031
                     /locus_tag="WIX60_000709"
     tRNA            9956..10031
                     /locus_tag="WIX60_000709"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9989..9991,aa:His,seq:gtg)
     gene            10167..10673
                     /gene="rfaE2"
                     /locus_tag="WIX60_000710"
     CDS             10167..10673
                     /gene="rfaE2"
                     /locus_tag="WIX60_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEZ5305007.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGDALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10670..12448
                     /locus_tag="WIX60_000711"
     CDS             10670..12448
                     /locus_tag="WIX60_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ5305008.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIYGLLNMIAAEGREYEHI"
     gene            12460..13326
                     /locus_tag="WIX60_000712"
     CDS             12460..13326
                     /locus_tag="WIX60_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ5305009.1"
                     /translation="MKWLFILLVAINIAVFGGTVGYKLTLKQAGRIPEAQNAANNLQV
                     QPVAPTMPVVRNIPASGPVVQAASESDTGALLKQGDILSEEQAEQLRLKKEAEQKKLK
                     EKKQREEKARREKLAAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHV
                     ASRSVEKRTAQAKPADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSR
                     ENAQALQNRLAGAGFGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKIT
                     SLYGKLNLKSCK"
     gene            complement(14089..14877)
                     /locus_tag="WIX60_000713"
     CDS             complement(14089..14877)
                     /locus_tag="WIX60_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ5305010.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(15091..15285)
                     /gene="thiS"
                     /locus_tag="WIX60_000714"
     CDS             complement(15091..15285)
                     /gene="thiS"
                     /locus_tag="WIX60_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ5305011.1"
                     /translation="MNIILNGGPAELHGTTVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3665 a         3849 c         4248 g         3541 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
       61 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcggacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggc agcgtctggg cagaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgttaaa cgctaccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 acccttatcc gcgacctcga caccggcgaa accgttgccg acctcactta tcacggacag
      721 cgcgtctgcc tcgccaaagg cggcaaaggc ggcttgggca acatccactt caaatcgtcc
      781 gtcaaccgcg ccccgaaaca atccaccccc ggcgaagaag gcgaagcccg ttccctgcaa
      841 ctcgaactta aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccaa acttaggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg cgcggcagaa ggcgcaggcc tcggccatcg tttcctcaaa
     1081 cacttatcac gtaccggcct gctgctgcac gtcgtcgatt tggcgccctt cgacgaaacc
     1141 gtcaaccccg ccgaagaagc actcgccatc atcaacgaat tacgcaaata cgacgaagaa
     1201 ctctacggca aaccgcgctg gctggtactg aacaaactcg atatgcttga cgaagaagaa
     1261 gcccaaacgc gcactgccgc cttcctcgaa gccgtcggct gggactaccc caaacccgac
     1321 gaccgtttcc aatttgacat ggaaaccccg cgcctcttcc aaatcagtgc gctgacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgaccgagaa aaaacgtatc
     1441 gaagctgaaa aagcagaagc ggagaaagca gcggcaaatg ttgaaattat cgaacagcag
     1501 cctaaaacgg atacaggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggt aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatacctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggcggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctt tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgacgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgaa tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagaaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctagt ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcagccata ttaataatcc gcaactacgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga ttaatccata atcccatgac
     3361 caccatctac aacaccctaa cccgtcaaaa agaacccttt tcccccatcg accctgaaaa
     3421 cgtgcgtatg tacgtctgcg gtatgaccgt ttacgattac tgccatttgg gacacgcccg
     3481 cgtgatggtg gtgttcgaca tgattgcccg ttggttgcgc gagtgcggct atccgctcac
     3541 ttatgtacgc aacatcaccg acatcgacga taaaatcatt gcccgcgcgg ctgaaaacgg
     3601 cgagaccatc ggcaaactga ctgcgcgttt catccaagcc atgcacgaag atgccgatgc
     3661 tttgggcgtg ttgcgtccgg atattgagcc gaaggcaacg gaaaacattc cgcaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa atggcgacgt
     3781 ttactacgcc gtgcgcgagt ttgccgctta cggacaattg tcgggcaaat cgttggacga
     3841 cctgcgtgcg ggcgaacgcg tggaagtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 gttgtggaaa gccgccaaag caggcgaacc ggcgtgggaa agcccgtggg gcaacggccg
     3961 tccgggttgg cacattgaat gctctgccat gagtgaaaac ctgttcggcg ataccttcga
     4021 catccacggc ggcggcgcgg atttgcagtt tccgcaccac gaaaacgaaa ttgcccaaag
     4081 cgtcggcgcg acggggcata cctgcggtca tcatcacgcg caaacccacc acgggcaaag
     4141 catcgccagc cacgtcaaat actggctgca caacggcttt atccgcgtgg acggcgaaaa
     4201 gatgtccaaa tcgctgggca acttcttcac catccgcgaa gtgttgaaac aatacgatcc
     4261 ggaagtcgtg cgcttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gatgatgcaa aaggcgcgtt gacgcgtttg tatacgactt tgaaaaatac
     4381 gcctcccgcc gatccgatgc cgtctgaagc cggcgatgac tacacgcgcc gcttttatgt
     4441 tgcgatgaat gatgattttg atacggtgaa agcggtggcg gtgttgttcg aacttgccgg
     4501 cgaagtgaac aaaaccaatg acgcgcaact ggcaggccgt ctgaaagcct tgggcggcat
     4561 catcggactg ctgcaacgcg atccgaccga gttcctgcaa ggcggcgcgg cttcagacgg
     4621 tctctccaac gaagaaatcg aagacttgat cgcccggcgg aaacaggcgc gcgccgataa
     4681 aaactgggcg gaatccgacc gcatccgcga ccttctgaac gaacacaaaa tcattttgga
     4741 agacaacgcc ggcggcacga cttggcggcg cggttaaaat ccatcgaaaa gggtaaatgt
     4801 gccgcatttt gccctttttt gttcagacgg catttttatt gtgagaactt atttttatta
     4861 tgcttaaaat catttccgcc aacgtcaacg gcatccgctc cgcctacaaa aaaggttttt
     4921 acgaatacat cgccgcatcg ggcgcggaca ttgtctgcgt gcaggaactc aaagcgcagg
     4981 aggctgattt gtctgccgat atgaaaaatc cgcacgggat gcacggtcat tggcattgcg
     5041 ccgagaagcg cggttacagc ggcgtggcgg tgtacagcaa acgcaaaccc gacaatgtgc
     5101 aaatcggtat gggcattgaa gaattcgaca gggaagggcg gtttgtgcgt tgcgatttcg
     5161 gcaggttgag cgttatctcg ctttatttgc ccagcggcag cagcgcggaa gaacgccagc
     5221 aggtgaaata ccgtttcctt gatgcgtttt accctatgct cgaagcgatg aaaaacgaag
     5281 ggcgcgacat tgtcgtctgc ggcgactgga acatcgccca ccaaaacatc gacctgaaaa
     5341 actggaaagg caaccagaaa aattcaggtt tcctgcctga agagcgcgag tggataggca
     5401 aggttatcca caagctcggt tggacggata tgtggcgcac gctttatccc gatgtgccgg
     5461 gctacacttg gtggagcaac cgagggcagg cgtatgcgaa agatgtcggg tggcgcatcg
     5521 attatcagat ggttacgccc gaacttgctg ccaaagccgt gtccgcacac gtttataaag
     5581 atgaaaaatt ttctgaccat gcgccgctgg tcgtggagta tgactatgct gccgaataag
     5641 gttttgggta aatatgattg gaatgtggac ggaaaaacag gaataggcgc ggcatgggtc
     5701 gtcgctgcgt ttatcctgcc catgttggtg tgggcgatat ttatgttgtc gcggatgcaa
     5761 ggctggcttg cgccgaccaa ggcaaacccg atatgggcgt tggtgtggct gctgatttgc
     5821 ctgccctgcc tgctgattgc gtccaaatgt ttgggttgga aaggctggcg gcgggtggtg
     5881 aatatttttg cctgcctgac cgtctgcgcc atattgagcg tacccgcttc gctgctcatc
     5941 gcctttaccc tccgggacct gctgaaatag gcggggcggt atgcagggtt ttgaactcga
     6001 agatttgacg ctttggctga tacgcgatgc cggagaggat cagatgtgga tagaccgttg
     6061 ggcgatcagt tatcccgtcg tgcagatgtc cgaagcgtcg gccggtcaaa gcatagggga
     6121 atggcaggca gggcttcaga cggcatttga acgcatacgc ggcaaatacg tcgccgttgt
     6181 cgcacacggt gcgggcgcgg ccgcattttt ggcgtggctg tatcaggcag acatcctgac
     6241 acggaagaaa attgccaaca tcatccttgt accgcagcgt cccgatattt ttcccgacga
     6301 tgcggaacac gctttccaac gcgtccgctg tccctgccgt gccgcattgg ttgtacccga
     6361 acacggcggc gtgccgcacg gttgggcgca aaaacaggcg gatttgtgga acgcccgcct
     6421 gttggtttcc ccgcattcgg gcagtttgaa cggcatgctc ggcggctggc agtggggtat
     6481 gaagctgatg caggaaatgt tgctggcgtg aacgcttgcc ttatcggaat ttcggctgta
     6541 gcaggaagtc cgcaatcggg ctgttttcat tgacgtgcag gcgcatcagg ttcggataaa
     6601 gtcgggctgc cgcataccgc cggaaatgga atcctttatt ttttacgtcg gcagtgccgg
     6661 cagggcgtta tccggcaggg ttgcaaggaa aatgccgtct gaaacaggct tcagacggca
     6721 tttccattca aacagggctc acaggctgcc caacacgata cgcaatacgc ggcgcagcgg
     6781 ttcggcagcg ccccacaaaa gttggtcgcc gacggtgaac gcgctgatgt attcgccgcc
     6841 catgcccagt ttgcggatgc gtccgacagg gacggacagc gtgccggtaa ctttggcagg
     6901 agtcagctcg tggatgctgg cttctttttc attggggatg actttcaccc agtcattcgc
     6961 gcctgccaaa atcgtttcga tttcggaaac aggcaggtct tttttcaact tcagagtgat
     7021 ggcttggctg tggcagcgca tcgcgccgac gcggacgcac aggccgtcaa tcacggttgg
     7081 attgtcgctg cggccgagga ttttgttggt ttccacgccg cctttccatt cttctttgga
     7141 ctggccgttg cccaaatcca cgtcaatcca cggaatcagg ctgccggcga gcggtacgcc
     7201 gaagttggct ttcggatagt cttcgctgcg caggaaatcg gatactttgc ggtcgatgtc
     7261 gagaatcgag ccggcaggat cggcaagcgc gtccgccact tgggcgtgaa ccgcgcccat
     7321 accgctgatg agttcgcgca tgtttttcgc gcccgcgccg gaagcggctt ggtaggtcat
     7381 gctggttgcc cattcgacca aatcgttttg gaacaggccg cccaaagcca tcagcatcag
     7441 ggaaacggtg cagttgccgc caatgtagtt tttcacgccg tttttgagac cgttgtcgag
     7501 gacatcgcgg ttgacagggt cgaggacgat aatcgcgtcg tctttcatgc gcagtgagga
     7561 cgccgcgtca atccagtagc cgttccagcc gctgtcgcgc agggcttgga agacggattt
     7621 ggtgtaatcg ccgccttggc aggtaacgat gatgtccatt ttggcgagtt cggcaacatt
     7681 gttggcatct aataatgttt tagccgcctg accgaaatca ggggctgcgc cgccgacgtt
     7741 ggaagtggta aagaaaaacg cttcaggaat gtgggcgaag tcgttttctt ctttcatacg
     7801 ctgcatcaaa accgaaccga ccataccgcg ccagccgacg aaacctactt tcattgtgtt
     7861 gctccttgag gaaaaatcgt taataaaatt atgtatggcg taaaggaagc gttttaacgc
     7921 cgtattggga aaatgtaaag ttctttatcg ggatttcttt aagtattggc gttttcggtg
     7981 tcgtggcggt agaatcgggg tcagttaaga acaattatca ggaacggata tggaagaaaa
     8041 aaacgactac accgacgcgg catccgacaa ccgcaacggg caggaaattg aagtcggaat
     8101 tgccggtgca ggcgaccgga tacttgccgc gctgctgaac caattgttta cctttttggt
     8161 tttgttggtg ccgtttgccg gactgattgc tttcgccatc aagaatgaag gcaggattgg
     8221 cagcggcgaa gaaatattcg ggctgctttt gggcatgact tccttttggg taggactggc
     8281 aggcattttg gcgtacaccg tcatccaaat ctactatatg agccgggacg ggcagtcatt
     8341 gggtaagaaa atcatgagaa tccgtgtgtt gaaaaccgac ggccgcaatc ccggttttgt
     8401 cggcacggtt ttggtacgcg aaatcgcatg gtcggttttg gttgccatta ttgccgccgt
     8461 tatcggtctt gcagtaggtg acaacggaga aaacgccatc aacctgctgg cattccttgc
     8521 caactttgtc ctgctcttta tggtcaaacg cgaccgccgc acgctttacg acatactggc
     8581 ggatacggtt gtcgtcaagc tgcccaaata aacggacacg gcaaaatgcc gtctgaaagc
     8641 ctttcagacg gcatttttat cttcagaacc gctcaagcat cgtgcagtga agccgcgtgc
     8701 agggtgtttt ccatcaatgt ggcaatcgtc atcggaccca cgccgccggg aacgggcgta
     8761 atcatcgccg cccgttcttt tgccgtttca aattccacgt cgccgcacag gctgccatcg
     8821 tccaaacggt tgatgcccac atcaataacg accgcgccag gtttgatcca ttcgcctttg
     8881 acaaagttcg gaatgcctac gccgaccacc aaaatatcgg ctccggcaac ctcgtctgtc
     8941 agattttcgg ttgcgctgtg gcagaccgtt accgttgcgc gcgccagcag cagttccaaa
     9001 gcctgcgggc ggccgacgat attcgacgcg ccgaccacga ccgctttttt ccccttcgga
     9061 tcaatgccgt aagcttccaa aagcgtcatc acgcccttgg gcgtacacgg gcgcatcagc
     9121 ggcattttga ccgccagcct gccgacattg taaggatgga agccgtccac gtccttatcc
     9181 ggcgaaatac gttccaaaac cgcctggctg tcgaggtgct tcggcagcgg tagctgaacc
     9241 agaataccgt ccacttcgga atcggcattc aggcggtcga ccagtgccag cagttcttcc
     9301 tgcgatgttg attcgggcag ctcgtaagac agtgatttga tgccgcattt ttggcaggca
     9361 gttttcttgt tgcggacata aaccgcgctg gcagggtcgc ctccgaccaa aaccacggcc
     9421 aggcaaggcg tgtgcagatt gttctgttgg cgttgcgcca ccgcttcggc aaccgcctgc
     9481 aggcgttttt gcgaaacttc tttaccattg atcagttgtg ccgacatatc cgtccttttg
     9541 ttaaacaggt agaaaatgct gaaaattatc gcattttccg tttgttttta cagcaggatg
     9601 ccgaaaataa atttgccctg aaataaaata cggctgtttg gacggattaa atattttttc
     9661 aaatcaaaaa actatatttt tcatcttggt gtcttgaaaa taaatgttga cgcatctttc
     9721 cctgagttgt atagttcgct tcttcaagtc ggggcgtagc gcagcccggt tagcgcatct
     9781 gctttgggag cagagggtcg tgagttcgaa tcccaccgcc ccgaccaatc aatcagcacg
     9841 aatcggcatg agcgcccgta gctcaaccgg atagagcacc gaccttctaa gtcgggggtt
     9901 acaggttcga ttcctgtcgg gcgtgccaag aatttgattg aaaaccgttt ttatggtggc
     9961 tgtagctcag ttggtagagc cccggattgt gattccggtt gtcgtgggtt cgagccccat
    10021 cagccacccc agataaaggc ccgtactttt cggtacgggt cttttcccgt ttgcgcgtac
    10081 tgtccgcgcc gccggctttg cctttcgcat atgcttctgt atcatgtggg tttttctgtc
    10141 ttttatggtt tttgtcggag gctttgatgg ttgacgcttg gtctgtccct gattttgaat
    10201 cgaaaatctg tccgcccgag gcgttggcgg cgcgtttggc gttgttgccg cgcccgctgg
    10261 tgtttaccaa cggctgtttc gacatcctcc acagggggca cgttacttat ctggcgcagg
    10321 cgcgttcgat gggggacgcg ttggtgctgg cgttgaatac cgatgcttcg gtgcggcgtc
    10381 tgggcaaggg cggtgaccgc ccggttaatc ctttggagaa ccgtgccgcc gttgccgccg
    10441 ctttggaaag tgtggatttg gtaacgtggt ttgacgggga tacgccggcg gcgttgattg
    10501 aggcggtcaa acctgagatt ttggtcaagg gcggcgattg ggctgcggat aagattgtcg
    10561 gtgcggcaga aacgttggcg cgcggcggtc aggtgttttc aattccgttt ctgcaccaga
    10621 cttcgacaac gaagactttg gcaaaaatcc gtgcggcaga gggcggaaaa tgacggtttt
    10681 gaagctttcg cactggcggg tgttggcgga gcttgccgac ggtttgccgc aacacgtctc
    10741 gcaactggcg cgtatggcgg atatgaagcc gcagcagctc aacggttttt ggcagcagat
    10801 gccggcgcac atacgcgggc tgttgcgcca acacgacggc tattggcggc tggtgcgccc
    10861 attggcggtt ttcgatgccg aaggtttgcg cgagctgggg gaaaggtcgg gttttcagac
    10921 ggcattgaag cacgagtgcg cgtccagcaa cgacgagata ctggaattgg cgcggattgc
    10981 gccggacaag gcgcacaaaa ccatatgcgt gacccacctg caaagtaagg gcagggggcg
    11041 gcaggggcgg aagtggtcgc accgtttggg cgagtgtctg atgttcagtt ttggctgggt
    11101 gtttgaccgg ccgcagtatg agttgggttc gctgtcgcct gttgcggcag tggcgtgtcg
    11161 gcgcgccttg tcgcgtttag gtttggatgt gcagattaag tggcccaatg atttggttgt
    11221 cggacgcgac aaattgggcg gcattctgat tgaaacggtc aggacgggcg gcaaaacggt
    11281 tgccgtggtc ggtatcggca tcaattttgt cctgcccaag gaagtagaaa atgccgcttc
    11341 cgtgcaatcg ctgtttcaga cggcatcgcg gcggggcaat gccgatgccg ccgtgctgct
    11401 ggaaacgctg ttggtggaac tggacgcggt gttgttgcaa tatgcgcggg acggatttgc
    11461 gccttttgtg gcggaatatc aggctgccaa ccgcgaccac ggcaaggcgg tattgctgtt
    11521 gcgcgacggc gaaaccgtgt tcgaaggcac ggttaaaggc gtggacggac aaggcgtttt
    11581 gcacttggaa acggcagagg gcaaacagac ggtcgtcagc ggcgaaatca gcctgcggtc
    11641 cgacgacagg ccggtttccg tgccgaagcg gcgggattcg gaacgttttc tgctgttgga
    11701 cggcggcaac agccggctca agtgggcgtg ggtggaaaac ggcacgttcg caaccgtcgg
    11761 tagcgcgccg taccgcgatt tgtcgccttt gggcgcggag tgggcggaaa aggcggatgg
    11821 aaatgtccgc atcgtcggtt gcgctgtgtg cggagaattc aaaaaggcac aagtgcagga
    11881 acagctcgcc cgaaaaatcg agtggctgcc gtcttccgca caggctttgg gcatacgcaa
    11941 ccactaccgc caccccgaag aacacggttc cgaccgctgg ttcaacgcct tgggcagccg
    12001 ccgcttcagc cgcaacgcct gcgtcgtcgt cagttgcggc acggcggtaa cggttgacgc
    12061 gctcaccgat gacggacatt atctcggggg aaccatcatg cccggtttcc acctgatgaa
    12121 agaatcgctc gccgtccgaa ccgccaacct caaccggcac gccggtaagc gttatccttt
    12181 cccgaccaca acgggcaatg ccgtcgccag cggcatgatg gatgcggttt gcggctcggt
    12241 tatgatgatg cacgggcgtt tgaaagaaaa aaccggggcg ggcaagcctg tcgatgtcat
    12301 cattaccggc ggcggcgcgg caaaagttgc cgaagccctg ccgcctgcat ttttggcgga
    12361 aaataccgtg cgcgtggcgg acaacctcgt catttacggg ttgttgaaca tgattgccgc
    12421 cgaaggcagg gaatatgaac atatttaagg aatacagaga tgaaatggct atttatcctt
    12481 ttggttgcga ttaatattgc cgtattcggc ggtacggtag gttacaaact gacactgaaa
    12541 caggccggca gaataccgga ggcacagaat gccgcaaaca atttgcaggt tcaaccagtt
    12601 gccccaacta tgccggttgt tcggaatatt ccagcatccg gtcctgtcgt tcaggcggca
    12661 tctgaatcgg atacaggcgc actgctcaaa cagggcgaca ttctgagcga agaacaggcg
    12721 gagcagttgc gcttgaaaaa agaagcggaa cagaaaaaac tgaaagagaa aaaacagcgt
    12781 gaagaaaaag cccgccgcga aaaactcgcc gccgaaaagg cgcaggcgga acgcgaaaac
    12841 ggcgcggcgg atgccttatg cgccgcgcag gcaagcctca cgatggacga agatgactac
    12901 caccgcatca aaggactttt gggcaaatgg tcgcacgttg ccagcaggag cgtcgaaaaa
    12961 cgcaccgccc aagccaaacc tgccgacaaa acctaccgcg tcgtcctgcc cgtttccgcc
    13021 gatgccgaaa atcaggcggc ggagctgtct gccaaaggtt tcaaccccat accgtttgac
    13081 ggcgcattga gtttgggtgt cggcaacagc cgggaaaacg cccaagccct gcaaaaccgg
    13141 cttgccggcg ccggattcgg cggggcgcat attgtcgaac actttgccga agccgacagg
    13201 caggacgatt ctttgagcgt gtcgcgtatg acggttttgt ttaccggcgt gaatgccgcc
    13261 gatgcggacg aaattcgtaa aatcacgtcc ctatacggca aactgaacct caagtcttgc
    13321 aaataggcgc gaaagccgga cgataaacgc caaacccacc gcgaaggtgg gtttggcgtt
    13381 tcggttttga gtatctgata tataaaaagg gaatgtatgt ctcgtatatg ccgactccaa
    13441 gtgtgaaagt gatgatgggg aaatatggtg tttaatggtt cggaacggac ggcttccgag
    13501 tggttttgat tatacgctaa taataataat tatcaataaa aatttaaaaa taaatgcaga
    13561 ttttttgtaa tacgatgaaa aataaacaaa ttaacctgtc cgccgatgtt tgtgattcgg
    13621 gtcggcaaac tttcattgtg tcggacaaat cggggtcggc aggcatagct gagggcttcg
    13681 tgcctgacgg aaataaggtt gcccgtgttt gaacatccgt ccgatttggc gcggacaggg
    13741 ttgtcattcg ggggtgtttg agcgtggatt cggggatgcg aaagcggtaa aaccgttgcg
    13801 gatgaaggct tacggacggc tttaggtatt tttaagtgta ttcaaacaac aacaaaacgc
    13861 ttcttctatt gccgtattta gggcttgcgc ggcagcctgc acaccgtgga ggccgagtgt
    13921 ttcgttggtt gcggcatcgg gacgggcggc aaggttttcc ggccggtttt tccatttttt
    13981 ccgacaaagc cgtgatgccg gggaatggcg gtttgtccgt ttcaggccgc cgccgttttg
    14041 gaccgcgatg ccgtctgaag ccttcagacg gcatttttgc tgcctttttc aatattccgc
    14101 cgaatgccaa aacggttgtc cgactgtcgg cgtgctggct tgcgctttgt cgcgtgcttc
    14161 gaccggtccg gcttcaaatg ccagccgtcc ggattcgacg gcgagtgcga aggcgcgtgc
    14221 catattgacc ggatcgccgc tgcgggaaac ggcagtattc aaaagcacgc cgtcaaagcc
    14281 ccattccatc acttgtgccg cctgtgaggg caaacccaag cccgcgtcga taatcagcgg
    14341 cgtgtcgggc aggcgttcgc gcaggacgtt caacgcgtag gcgtgaaccg cgcccaaacc
    14401 cgtgccgatc ggggccgccc acggcatcaa cgcctgacag cccgcgtcga gcaggcggcg
    14461 gcaggcaatc aggtcttcgg tgcaataagg cagcactttg aagccgtctt taatcaggat
    14521 ttccgccgct tcgacaagct ggaacacatc cggctgcaag gtgtcgtcat ctccgatgag
    14581 ttccaatttt atccaatcgg tttcaaacac ttcgcgcgcc atttgcgccg tcgttaccgc
    14641 ttcctgcacg ctttggcagc ctgccgtgtt cggcaggacg ggaacgccgg tttcttgaag
    14701 cagcgaccaa aacccctgac cgtgcgcctc gccgccgctt cccgcgcggc gcagcgagac
    14761 ggtaatcatc gcaggctggg cggtttggat ggattgtttg aggatttcgg gggtcgggta
    14821 ggcagccgtg ccgagcagca gccgcgaggg gaaagtttcg ccgtataggg tgagcataat
    14881 gggttccttt gtatagtgga ttaacaaaaa ccagtacggc gttgcctcgc cttagctcaa
    14941 agaaaacgat tctctaaggt gctgaagcac taagtgaatc ggttccgtac tatccgtact
    15001 gtctgcggct tcgtcgcctt gtcctgattt aaagttaatc caccataacg ttgttttggg
    15061 gacaggggtc gtctgaaaag gcaaaaccgc ctagccgccg accaccggcc gcacgatatc
    15121 gattttgtcg ttttcgttta aaaccgtttc cgcatacgcg cctttgggga cgaaaacggt
    15181 gttgaccgcc acggcaaagg gcttttgcgg cgcggtttgg gcgatgaggt cggcaacggt
    15241 cgtgccgtga agttcggcgg gtccgccgtt taagatgatg ttcatggttt ctcgatgttt
    15301 ctg
//
LOCUS       DAWIVB010000032        14612 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWIVB010000032 DAWIVB010000000
VERSION     DAWIVB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.032"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(78..293)
                     /gene="nqrM"
                     /locus_tag="WIX60_000715"
     CDS             complement(78..293)
                     /gene="nqrM"
                     /locus_tag="WIX60_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ5305012.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(319..1374)
                     /locus_tag="WIX60_000716"
     CDS             complement(319..1374)
                     /locus_tag="WIX60_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ5305013.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(1527..2744)
                     /gene="nqrF"
                     /locus_tag="WIX60_000717"
     CDS             complement(1527..2744)
                     /gene="nqrF"
                     /locus_tag="WIX60_000717"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ5305014.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(2758..3351)
                     /gene="nqrE"
                     /locus_tag="WIX60_000718"
     CDS             complement(2758..3351)
                     /gene="nqrE"
                     /locus_tag="WIX60_000718"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ5305015.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIAEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(3355..3981)
                     /locus_tag="WIX60_000719"
     CDS             complement(3355..3981)
                     /locus_tag="WIX60_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ5305016.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(3981..4757)
                     /locus_tag="WIX60_000720"
     CDS             complement(3981..4757)
                     /locus_tag="WIX60_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ5305017.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKTEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(4750..5982)
                     /locus_tag="WIX60_000721"
     CDS             complement(4750..5982)
                     /locus_tag="WIX60_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ5305018.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQNIANDWHYAFAN
                     ALGINMSSEAGVSDKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(5985..7328)
                     /locus_tag="WIX60_000722"
     CDS             complement(5985..7328)
                     /locus_tag="WIX60_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ5305019.1"
                     /translation="MIKIKKGLNLPIAGRPEQAVYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVITIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDTDNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(7678..9099)
                     /locus_tag="WIX60_000723"
     CDS             complement(7678..9099)
                     /locus_tag="WIX60_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ5305020.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(9114..9404)
                     /locus_tag="WIX60_000724"
     CDS             complement(9114..9404)
                     /locus_tag="WIX60_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEZ5305021.1"
                     /translation="MQIYVKINSFFVFDRLFIKDIYLFVLNKIIYQKEHIFPSTAIRL
                     PAAFRFGKLVYPDYLLYNQSENFFQKQTGSTVRLCPGGTVPVSLPRAAQGSA"
     gene            complement(9461..9820)
                     /locus_tag="WIX60_000725"
     CDS             complement(9461..9820)
                     /locus_tag="WIX60_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ5305022.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFYRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(9817..10422)
                     /locus_tag="WIX60_000726"
     CDS             complement(9817..10422)
                     /locus_tag="WIX60_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ5305023.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(10469..10951)
                     /locus_tag="WIX60_000727"
     CDS             complement(10469..10951)
                     /locus_tag="WIX60_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ5305024.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      11154..11251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11462..12562
                     /gene="gcvT"
                     /locus_tag="WIX60_000728"
     CDS             11462..12562
                     /gene="gcvT"
                     /locus_tag="WIX60_000728"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ5305025.1"
                     /translation="MTALKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            12724..13110
                     /gene="gcvH"
                     /locus_tag="WIX60_000729"
     CDS             12724..13110
                     /gene="gcvH"
                     /locus_tag="WIX60_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ5305026.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDSLLTAEQYAGEVD"
     gene            13262..14509
                     /locus_tag="WIX60_000730"
     CDS             13262..14509
                     /locus_tag="WIX60_000730"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ5305027.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         3540 a         3624 c         4024 g         3424 t
ORIGIN      
        1 accttagaga atcgttctct ttgagctaag gcgaggcaac gctgtactgg tttttgttaa
       61 tccactatat ttcggcgtta acggtcaacc cgtatgccgc ctgcctgttt ttcttcatcc
      121 agtttctttt gcagggtgtc gcaaggtgtg tcgcagtcgc acattttttt catacccaag
      181 gcagtaatgc cgccgcaact gcctttgatg ctgcgtttgg agaaaatata gccgaccgcc
      241 ataccgatga tgacggtcag gaagatgccg aaggtaagga gcagggtttt catggtgttt
      301 cctaatcggt ttgtatgttt agcggagcag tttttcaaat tcggaagaca tggcggtgcg
      361 gtagccgcct ttatccctga caatcaggaa aacagcgagt ttttcgcgct ctgccagctt
      421 taaggcttcg gtttcgccca atacgaataa tcctgtggac aagccgtccg ccgtcatcgc
      481 actgtctgcg accacgctga tggaggcgag gttgtggctg atgggtcgtt tgttgttcgg
      541 gttgatgata tgggagaggc gtttgccgtt tttatcgacg tggaaaatac ggtaatcgcc
      601 ggaagtggca agcgaacggt tgttcagcgg gacgataatc tgcgtattgc cgccttggac
      661 gatattgggc tgctcgatac cgatgcgcca cggttcgccg cgcgcgtttt tgcctttgcc
      721 gtgcaactcg ccgccgattt cgaccagata attttgaatg ccgtattttt ccagttcgcc
      781 cgcaacttta tcaacgccga agcctttggc aatcgaagat aaatccaaat aggccttggg
      841 gtgggttttg ctcaaggaag cgtaatcttt gccttgtttc aaaatgattt tgtctatgcc
      901 cgtataagat gccgcctgtt tgatttgttc cggcgacggt tcacgggtaa cggatttgtc
      961 ggggccgaat ccccaaaggt tgaccaaggg gccgacggtt acgtccagcg cgccgtgtgt
     1021 caggcggttc aggcggacgg cttcggcagt aacgtgtgcg aagtcgcttg aaatgcggag
     1081 gggcttgccg gctgtgtgtt ggttgaaccg gctgatttcg gagtcgggct gataggtgga
     1141 catctgccgg ttgacttctt taagcgcgtc atcgatgcgt ttttgtattt cggcaggtga
     1201 ggggagtttg tcccgattat ttgaaaggta tttgacggta taggtcgtgc ccatcgtttc
     1261 gccttgcagg gtaacggttt gcgcggtttg ttccgaacag gcgttcagga agatgaaacc
     1321 cagggcaaat atcaagacgc ggataaagtt cggcaggcgt gtttcagacg gcatagtgtt
     1381 tgacggtttt ggcaaatggt ttgaattata tcgcaaaacg gccggtatgt ttctatgccg
     1441 atgccgtctg aagggtgttc ggatggcatc ggcatagaaa aaggaagaaa ccgaggtttc
     1501 ttccttttgt atttgaagcc gaatatttaa ccgccgaaat cgtccaagag gatgttttcg
     1561 tcttccacgc ccaagtcttt gagcattttg atgacggact ggttcataat cggagggccg
     1621 cacatataaa attcgcagtc ttccggtgct tcgtggtttt tcaggtggtt ttcgtaaacc
     1681 acgttgtgaa tgaagcccgt gtagccgtcc cagttgtctt ccggcagcgg gtcggacagg
     1741 gcgacgtgcc acgtgaagtt cgggaactct gccgcgagtt ggtcaaagtc ttcgacatag
     1801 aacatctcgc gtttggaacg tgcgccgtac cagaaggtaa tcttacgttt ggagttcaaa
     1861 cgtttcaact ggtcgaaaat gtgggaacgc atcggagcca tacccgcacc gccgccgata
     1921 aataccattt cggcatcggt gtctttggcg aaaaattcgc cgaacgggcc ggaaatcgta
     1981 actttgtcgc cgggtttgag cgaccagatg taggacgaca tttgtcccgg aggcgcatca
     2041 ggtacgcgcg ggggcggcgt ggcgatacgc acgttcagca taatgatgcc tttttcttca
     2101 ggatacgaag ccatagagta ggcacgcaaa atcggctcgt ccactttgga aacgtattgc
     2161 cacaaattgt atttgtccca gtcttcgtga tattccttag gaatgtcgaa gtctttgtag
     2221 gcaacagtgt gaggaggagc ttcaatttga atgtagccgc cggcgcggaa ggggacttct
     2281 tcgccttcgg gaatggcaag cttgagttct ttaatgaacg tggctttgtt atcgttggag
     2341 atgacggtgc attcccattt tttcacgccg aacacttctt cggggacttc gatgtccatg
     2401 tcggttttga cgttgacttg gcacgacaga cggcagcctt cgcgtgcttc gcgtttgctg
     2461 atgtgggaca gctcggtcgg caggatgtcg ccgccgccgc tttttacgac gacgcggcat
     2521 tgtccgcacg aaccgccccc gccgcaggcg gaggggataa agatgccttc gttggcaagc
     2581 gcgcccaaga gtttgccgcc ggcgggcatc gtcagctctt tttcgccgtt gactttgatg
     2641 gtgatgtcgc cttcgctgac cagtttggat ttggcaaaca gaatcatcag tgccaaaacc
     2701 aaaacgatga cggtaaacat cacgatacct aaaataatct ccataccgat ccctttctta
     2761 taactggatg ccagagaacg acataaacgc catcgccatc aggccggcgg cgataaaggt
     2821 aatgcccaag cctttgaggc ctttgggagc gtccgaatat ttcatttttt cggtaatgcc
     2881 cgccaaagcg acaatcgcca acatccagcc caagcccgcg ccgaagccgt atacaacgga
     2941 ctcgccgaag ttgtattcgc gttgcgccat aaacgatacg gcgccgaaaa tcgcgcagtt
     3001 cacggtaatc agcggcaggt agatgcccaa tgcgttatag agggcgggga cgaatttatc
     3061 caagaacatt tccaaaatct gcaccaaagc ggcaatcacg ccgatgaagg tgatgaattt
     3121 caaaaaggtc aaatccacgc cttcggcaat cgcgccgtct ttgagcagcg agtaaacgag
     3181 ttggttgaca gggacggaca gcccgagtac gaaaattacc gccacaccca aaccgaatgc
     3241 ggtggatact tttttggata ccgccaaaaa cgtgcacata cccaaaaaga aggatagtgc
     3301 catattttca atgaagacgg atttgatgaa gaggctcaaa tagtgttcca tagcttattc
     3361 ctccgcctgt tcgggtttcc aggtacgcag tccccaaatc aaaaagccga tgatgaagaa
     3421 cgcgctgggg gcgagcagga acaagccgtt ggtctgatac cagccgccgt cctgcacggt
     3481 ttggaaaacg gtgtagccca agagtttgcc cgagccaatc agttcgcgga cggtggcgac
     3541 gacaagcagc attatcccgt agcccgcgcc gttgccgatg ccgtcgatca ggctttccag
     3601 cggcggctct ttcatcgcaa atgcttcggc gcggcccatc acgatacagt tggtaataat
     3661 cagaccgacg aatacggaaa gctgtttgga caattcgtag gcaaatgcct gcaagagttg
     3721 gtcgaccagc gtaaccagcg acgcgataat cgccatttgc acgataatac ggatgctgtt
     3781 ggggatgtag ttgcgtacca gcgaaatgaa gaagctggaa aaaccggtta ccaaagctac
     3841 ggaaataccc atcacgatgg ccgtctgaag tttggtggta accgccaaag ccgaacaaat
     3901 acccaaaacc tgcaaggcaa tcgggttgtt gtcgataaag ggtgaaaaca tcaaatgttt
     3961 caagcgtttc atatcagcca ttattgcgct cctgctgatt tcaatttgtt caggtagggg
     4021 atatagccgt tttcgccgaa ccagtaggcg aacgaacctt gcacgccttt ggatgtcagc
     4081 gatgcgccgg agagggcatc tacgccgtgt tctttgtccg aacccgcgcc tttgccgacg
     4141 tgcagggcga gtttgccttg tccgtcaaac agttttttgc cgacgaattt ttgctgccac
     4201 aacggattgc cgatttcgcc gcccaagccc ggggtttcgc cttgttcgta gtaggtaatg
     4261 ccgttgatgg tgttgccgtc gggctggatg gcgacaaagc cgtacatgac cgaccacaaa
     4321 ccgttaccgt gcataggcag gatgatttgc ccgattttgc cgtcttcgcc ttttaccaaa
     4381 taaacctcgg tgtatttggc acggcttttg atgcctgcca aatcgtcttc ggttttgatg
     4441 cggatgcttt gggcagggtc tttgcctgcg atgcgcgcgc tgaagtcttt aggcgcatcg
     4501 gcgacgtatt cgccggtcgc caaatcgaca acacgttgct cgatacgctc ggcaaaggtt
     4561 ttaccgatgt cggtgtcctt atccatcaaa ccggctacgc tcaagatata gccttgtttg
     4621 tcttggagtt tttgtttctc ttggatgggt ttcaagccga cgaccgcacc ggcaacgatg
     4681 accgagcaaa tcaggctgac cgccaacacg acaatcagcg tgccgctgaa gctgtcttta
     4741 tcgaatttct tagccattgc tgcgcgcctt tctgcgtttg atgttcgctt gtgcgacgaa
     4801 atagtcgaaa atcggggcaa acaggttggc aaacagaatc gccaacatca tgccttcggg
     4861 gtaagccgga ttgaccacgc ggattaatac gcacatcaca ccgatcagtg cgccgtacca
     4921 ccatttgccg acattggtaa aggaagcgga aacagggtcg gtcgccataa acagcatacc
     4981 gatggcgaag ccgccgacca ccaagtgcca gtaccaaggc atagcaaaca tagcgttggt
     5041 gtccgaaccg atgaagttga acagcgaaga catcgcaatc ataccgatca tcacgccggc
     5101 aataatgcgc caagaagcga tgcgggcaaa cacgataaac gcgccgccga ttaagagtgc
     5161 caaagtggag acttcgccaa tggagccggg cagtttgccg ataaacgcgt ccatccaagt
     5221 gatggtttga ccggttacgg cgtttttcag gccgtctgca ccgtgtgccg cccattgcgc
     5281 cagtgcggtt gcgccggaat agccgtcaac cgccgtccaa accgcatcgc cgctcaagtt
     5341 ggcagggtag gcgaagaaca ggaaagcacg gcctgccagc gcagggttca tgaagttttt
     5401 acctgtaccg ccgaatactt ctttcgcaac cacaacgccg aaagaaatac ccaaagccgc
     5461 ctgccacagc ggcagcgtgg gcggaacgat taaggcaaac agaatcgaag taacgaagaa
     5521 accttcgttg atttcgtgtt tgcgcacggt ggcgaacaaa acttcccaga aaccgcccac
     5581 aacaaataca gtcgcgtaaa tcggcaggaa gtaaatcgcg ccaaacagca ttttgtccga
     5641 cacgcccgct tcagacgaca tattgatgcc caaagcgttg gcaaaggcgt aatgccagtc
     5701 gttggcgatg ttttgttgca gcaaatcagg cgttaacgca ccgaatgcct gcgcgccgac
     5761 gttgtacata ccgtagaaca tggcagggaa caaagccagc cacaccaaaa tcatcatgcg
     5821 cttggagtcg agcgcgtcgc ggacgtgcgc cgctttgcgc gttaccgcgc cggatgtgta
     5881 gaaaattgtc gccgcagctt cgtagagggc ataccatttt tcatgtttgc cgcccggcag
     5941 gaagtgcggt tcgatttttt ccagaaaatg tttcaagccc ataatcagcc ttccttctca
     6001 atggtttcca gcactttgcg caacagcggg ccgtattcgt atttgcccgg gcagacgaag
     6061 ctgcacaaag cgaggtcttc ttcgtccaat tccaagcaac ccaatgcctg cgcgctgtcg
     6121 gtatcgccga cgattaaatc gcgcaaaagc agggtgggca ggatatccaa gggcatcacg
     6181 cgctcgtaag taccaatcgg caccatggcg cggtcgccgc cgttgacggc tgtgttgaac
     6241 ttgaagagtt tgtttttcag gaaatggccg agggttgtac gcgtgatgga gtatttgtcc
     6301 ggctgcggcg caacccagcc gaacagctct ttgctgcggc cttcttcgat aacggaaatc
     6361 tgattgtggt agcgtcccaa ataatcgtgc gcgccttgtg taatcgcgcc gttcaatacc
     6421 gaaccggaaa tcacgcggtt gtctgtgtca accaattcgc ccgcagtaat ttgcgatact
     6481 ttcgcaccca aaacggtacg caagaggcgc ggtttgttga cttgagaacc acctagggca
     6541 atcacgcgct cggtgttcag acggcctgtt gcaaacaaac ggccaatggt aattacatct
     6601 tgataattga tggtccacac ggttttattc gcgccgaccg gctcgatgaa atgaatgtgc
     6661 gtgccactca aaccggcagg atgcgggccg ccgaattcat gtgtttcgat gttggcagca
     6721 ttttcagacg gcacgtctgc gccagctgcc ttacaaacat ggattttgcg ttcggtcaaa
     6781 cggctcaata ccaacaggcc gcgtttgaaa tcctcggcgg cttctttgat aatgaccgta
     6841 gggtcggcag ccagcggatt ggtgtccatc gcattgacga agatggcgaa cggctcggca
     6901 tcgacggcag gaattttgct gaacggacgg gtgcgcagcg cagtccacaa accggattgg
     6961 atcaggttgc ggcgcacttc ttcgccgctt aagtttgcca gcgcttcagg tgcgtagcgt
     7021 tcaaactcga tttcgtcgtt gccttcaacg gcaatcacga ctgactgaag tacgcgcttt
     7081 tcgccacggt gaatcgcggc gattttgcct gaagccggcg cagtaaacac cacgcccgga
     7141 ttctttttgt cttcaaacag cacttggcct tttttgacgg catcgccttc cttgactttc
     7201 atcgaggggc gcataccggc atattcttcg ccaagcaacg cgacttcggt aatggccggg
     7261 ccgtcgtaaa cggcttgctc cggtctgccc gcgatgggca ggtttagacc ttttttgatt
     7321 ttaatcatat atttgcatta cttgtgatgg ttaaggtaaa aacggcgtgt tttgataccg
     7381 tgtcgcgtgg catcaaaagc attgaataaa ttaatgtagc aaagtgttag attctatcag
     7441 gaattgtacc tgtttgtcag atttgctgct tttttccttg cggaagccgt ttttatagtg
     7501 gattaaattt aaaccagtac ggcgttgcct cgcccttgcc gtactatttg tactgtctgc
     7561 ggcttcgtcg ccttgtcctg atttttgtta atccactata aattgtcgga aggggggata
     7621 ttgatttgat tatgccggaa tttaaaatgc cgtctgaatg ttcagacggc atagcgttta
     7681 cagcagtttg aaaacgaaaa agataagggt atgtacgatg aagacgggtg tcaggaaggc
     7741 gaccgaccac atcatatagc cgaagaaagt cggcatcggt acgccgcgct gttcggcaat
     7801 ggccttgacc atgaagttcg gtgcgttgcc gatgtaggtc agtgcgccca tgaataccga
     7861 acccatagaa accgccagca gcgaatgaaa cagggtaccc gtcatcaagg cttgggcatc
     7921 gccgcccgcc atattgaaaa aaacgagata agtgggcgcg ttatccaaga atgccgacaa
     7981 tatgccgctc atccaaaaat acatcacatt aatcggatga cctgccgtat cgtgaaccag
     8041 cgataccacc ccgcccagcg cgcctgcctc gcctgctttc agaatgctca ggacgggaaa
     8101 gatggtgatg aagatgccga ggaagagttt gcccacttcg gcgatgggtt caaagttgaa
     8161 ttcgttgcct gcgcggactt gtttgggcgt gattgccata gatacggcgg tcaatgcaat
     8221 caggatgaca tcgcggacga ggttttgcag ggcgtaacgg ctgccgagga tttcaaatcc
     8281 cgggtgttcg ggtttccaaa ggccggacat tagaaccgcg ccgaccacgc ccgaaagcag
     8341 gaggaagttc catttgccga agatggcgat tttttcgggt ttttcctgtt gtgccggcgt
     8401 atcttgtgca atgctttcct gtttgaagaa acggttgtcg atgaaataga aggcggtcaa
     8461 caggacagcg gtgctgatca ggacgggggc gaacatatgt ttgaccgtcc acatgaaatc
     8521 tacgcctttg aggaagccga ggaagagtgg ggggtcgccc aaaggggtca gaccgccgcc
     8581 gatgtttgca accaggaaaa tgaagaagat gacgatgtgc acgcggcggg tacggttttg
     8641 gttggctttc agcagcggac gaatcatcag cattgctgcg ccggtcgttc ccatgataga
     8701 ggcaagtgcc gtaccgacgg caagcagggc ggtgttgagc ttgggtgtgc cgttcaagtc
     8761 gccccaaacc aaaatgccgc ctgaaatggt gtacagggca agcagcagca ggatgaaagg
     8821 gatgtattct tcaacgagtg cgtgtgcgac ggtatggata ccggcggacg cgccaaaaac
     8881 caaactgaac gggatgagga agagcaatgt ccaaaaggcg gtaattttgc cgtaatggtg
     8941 atgccaggta tgcgaaaaaa acaagggacc caatgcgata gacagcaaaa tcagggcaaa
     9001 gggcaggccc cacagcaggt ttaggtttgc gccgtccaaa tctgcggcgt aaaccgatgc
     9061 tgggaaaagc attagtgaaa acaggggtag gtggcgcatc gtgtttcctc gattcaagca
     9121 ctgccttgcg cggcgcgtgg gagtgataca ggcaccgtgc cgcccggaca taggcggact
     9181 gtgctgcctg tttgtttttg aaaaaagttt tccgattgat tgtaaagtaa ataatcagga
     9241 taaaccagtt tcccaaaccg gaaggcggcg ggaaggcgga ttgctgtgct tgggaatata
     9301 tgttcttttt gataaataat tttatttaaa acaaataaat atatgtcttt aataaataat
     9361 ctatcgaaaa cgaaaaatga atttatttta acatatattt gcaatgaaac aggtttgccc
     9421 cccccccgtt tgtttgccct tatccctttc agtacggcat tcaagattcg ggcctgcgcc
     9481 acatccatat ggcgacaagg gaacaaaaaa ccgatgaaac cgccccgacc caccagcgtt
     9541 ggggaaactg ccaaaacatt atcaggcagg atgcggtcat catactgatg gcgaatattt
     9601 tggctttgcg cggcactgcg ccgttttgtt cccagttatg aaccatcggg ccgaaatagc
     9661 ggtgccggtg cagccagcgg taaaagcgcg gggatgcctt tgcccagcag gcggcggaga
     9721 gcagtacgaa cggcgtggtc ggcaacagcg gcaaaaaaat gccgatgata cccaacagta
     9781 gggaaatgca gccgcaggca attaaaagat aacgtatcat tttgaaatat ttttcttatt
     9841 gtgcggataa gggcaggatg tgataccgag ttttgcccag ccttcatgtc ccattttttc
     9901 cagcagggcg atattgcgtt cgaatattgc cgaagcgtcg ggaaaggctt gtgcggcttt
     9961 ggcaatgctg tcttcgcgga tgaggtgcag cgtcggatag ggagaacggt tggtgtagtt
    10021 gccaatgtcg tctgaatccg tgccttcaaa ttggaaatcg ggatgaaacg gggcgatttg
    10081 gacgatgcct tctaagccgt tttcgacaac ggcggcatcg gcaatgtcga gcatatcgtt
    10141 gaatacgtcg aaatcgggga atagggtcgg gtgaaccagc agggtggttt ccagttcggt
    10201 ggcgggtgta ttgcccagtc gctgcagttc ttcgtccaag tcttccaaaa aaccgtcaag
    10261 gtgtttggct tcgctgatcg cgatgcggac aaggttttta acgtgggggg ctttggcaaa
    10321 gggacacagg ttcagaccga tgacggcttt ttccaaccat tgtccggtgt gttcggcaac
    10381 agcatcttta ttttcggaag tattgatatt cattattgtc atgtaaatgt gtttgcagat
    10441 tgcacgtgcg ggaaaatcgg gaagggcact attccttcag caggtggttg agcggcaggg
    10501 aggtggtgtg tttgatttct tttaaaacaa agctcgattg cgcatcttgt acgccgtggt
    10561 gggacaggag cgtatccaaa acaaaatggg aaaacgcgtt catatcggta aaaaacgcct
    10621 gaagcaggta gtcggtttcc cctgtcaggg cgaagcagct caagacttca ggccattttc
    10681 gaaccgatgc ggcaaagtct tcccgcgcgt cttttgcttt gcggatggaa acgcggataa
    10741 atgcctgaag tcccaagttg acagattccg gagacagcag cgcggcatat tggcggacga
    10801 taccggcatc ttccaactgc ttcagacggc gcaggcacgg agaaggcgaa agtgcgacac
    10861 gttcggacag ttcgacattg gtcagcctgc cgttttcctg gagaacctgt aagattttaa
    10921 tatcggtttt gtctaaagtg agttggggca tatttgcgtt ccgttttaag gaattcggat
    10981 tgtctgtccg tatgtttgcg gcaatccgca cagatggaga ccatattaac atataaaaag
    11041 ttataccgtc atccgggaca aattttgttt tcggaaaatc atgtgaaaac agaggcggtc
    11101 ggtttgcatc tctttaagac ggcttgccca aaccgccgat tcaagacata atcgggaaat
    11161 gtgcaggaga gtgttacacc caactacaat gtaaccaccg aaggcgcaga cacccttaaa
    11221 tcgctcaggt atcagggact gcacattgaa acaaacaatc tggagagcgg cgttggaata
    11281 acgtccaccg aaggggagaa ggccgtctga accaccattc agacaaccgc gcaaagcagt
    11341 gagcagactg gtttgccatc atgcggatac agccgaaaat ctcaggttca aggacagata
    11401 gggtcatccg cgcacaggtg cgcgggcggc atctgaacaa aaaatccgga gaaacttgag
    11461 aatgactgct ctgaaaacca ccccatttca tcaagcccat caagatgcag gcgcgaagct
    11521 ggtcgatttt gccggctggg agctgcccat ccattatggt tcacaaatcg ccgaacacga
    11581 agccgtgcgc accgacgccg gtatgtttga cgtatcccat atgctcgtta ccgacgtagc
    11641 aggcgcaaat gccaaagcct ttttccgcaa attgattgcc aacgatgtcg ccaagctcgc
    11701 ttttgtcggc aaagcccttt attccgcttt gctcaacgac aacggcggtg tgattgacga
    11761 cttaatcgtt taccgcacca atgaagccga aacccaatac cgcatcgtgt ccaacggcgc
    11821 gacccgcgaa aaagacacgg cgcaattcca caaagtcgga caagagttcg gcgtcgcctt
    11881 caatccgcgc tacgacctcg gcatgctcgc cgtacaaggc cctaaagcca ttgaaaaact
    11941 cctgaccgtc aaacccgaat gggcagatgt cgtccataac ctcaaaccgt tccaaggcgc
    12001 ggatttgggc aacgactggt ttgtcgcccg caccggctac accggcgaag acggcgtcga
    12061 agtcatcctg cccggcaccg aagccgtcgc attcttcaaa gccctgcaac aagccggcgt
    12121 acagccctgc ggcctcggcg cgcgcgacac cctgcgcatg gaagccggca tgaacctcta
    12181 cggcaacgat atggacgacg acaccagccc gctcgaagca ggtatgggtt ggaccgttga
    12241 cttgaaagac gaaagccgcg acttcgtcgg caaagccgcc ttgctggcat tgaaagaaaa
    12301 aggcgttgcc gtcaaacagg tcggcctgtt gctcgaaaaa ggcggcatcc tgcgcgcgca
    12361 tatggaagtg ttgaccgaca aaggccaagg cgaaaccacc agcggcgtat tctccccaag
    12421 cctgaaacaa tccatcgcca tcgcgcgcgt accgaaagat tttgacggcg ataccgccaa
    12481 agtgctgatg cgtggcaaag aagtggacgt gcgtgtactg aagctgccgt ttgtccgcaa
    12541 cggacagaaa cagtttgatt gatgcggttt cagacggcat tttcatttca tatgccgtct
    12601 gaaagcaggt tttaattgtt gtccgatacg gacgtttgta gaaagcattg aacaaggcat
    12661 ctgtggatat tgattcatgc agatgccgtc tgaaaataac ccctatcaat ggagtatcaa
    12721 accatgagca acaacatccc ggccgaactg aaatacgttg ccagccatga atggctgcgc
    12781 cttgaagaag acggtacgat taccgtcggc attacccacc acgcgcaaga gctgttgggc
    12841 gacatcgtgt tcgtcgagct gcccgaagtc ggcgcgaacc ttgccgctga agagcaagcc
    12901 ggtgtggttg agtctgtaaa agccgcgtcc gacgtgtacg caccgattgc aggcgaagtc
    12961 gttgccgtca acgaagattt gccaagcgct ccggaaactg ccaacagcga tccttacggt
    13021 gcaggctggt tcttcaaact caaaccggca aacgttgccg attacgacag tctgctgact
    13081 gccgaacaat acgcgggcga agtggattaa accgcccggc tgcctgacgg caaccgccgg
    13141 acaaacggaa actgcacctt cagacggcat ttttgcggtc ggaggtgcag ttttttgtcc
    13201 gtgttttaag gaagcagtta ggctataata acggtctata ttcatcttta ccgatttttt
    13261 catgcaactt accgctgtcg gactcaatca tcaaaccgca cctttaagca tacgggaaaa
    13321 gctggcgttt gccgccgccg ccctgcctaa agccgtccgc aatcttgccc gaagcaatgc
    13381 ggcaacggag gcggtaatcc tttctacctg caaccgcacc gagctttact gcgtcggtga
    13441 ttcggaagaa atcatccgat ggcttgccga ttaccacagt ttgccgattg aagaaatccg
    13501 tccgtatctg tacgcgctgg atatgcagga gactgtgcgc catgctttcc gcgtcgcctg
    13561 cgggctggat tcgatggtgt tgggcgagcc gcagatttta ggacagatta aggatgccgt
    13621 tagggttgct caagagcagg aaagtatggg taagaaactc aatgccctgt tccaaaaaac
    13681 cttttccgtt gctaaagagg tccgtaccga tactgccgtc ggcgaaaact cggtttccat
    13741 ggcttccgct tccgtcaaat tggcggaaca gatttttccc gacatcggcg atttgaatgt
    13801 cttgtttatc ggcgcaggcg aaatgattga gctggttgcc acttattttg ccgccaaaag
    13861 tccccggctg atgacggttg ccaaccggac gctggcgcgt gcacaggagt tgtgcgacaa
    13921 gctcggtgtc aacgccgaac cgtgcctgct gtccgatctg cctgccattc tgcacgatta
    13981 cgacgtagtg gtttcttcaa cggcaagcca gttgcccatt gtcggcaaag gcatggtgga
    14041 gcgtgcattg aaacaaaggc agagtatgcc gttgttcatg cttgatttgg cagtgccgcg
    14101 tgacattgaa gcggaagtcg gcgatttgaa tgatgcctat ctttatacgg tggacgatat
    14161 ggtcaatatc gtccaaagcg gcaaggaggc aaggcagaag gccgccgccg ccgccgaaac
    14221 gctggtgtcc gagaaagttg ccgaatttgt caggcagcag cagggcaggc agagtgtccc
    14281 cttgattaag gcgttgcggg acgagggcga gaaagcgcgc aaacaggtgt tggaaaatgc
    14341 catgaaacag cttgccaaag gcgcaacggc agaagaggtt ttggaacggc tgtccgtcca
    14401 actgaccaac aagctgctgc attcgccgac ccaaaccttg aataaggcgg gggaagaaga
    14461 taaagatttg gttcatgccg tcgcgcagat ttatcatttg gacaaataac ggtgcgccgg
    14521 gaaaaatgcc gtctgaagag gtttcagacg gcattttttt gtgccgcctg acaacatcgt
    14581 gaaatcccac attatatcga tgtaatcaca aa
//
LOCUS       DAWIVB010000033        14569 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWIVB010000033 DAWIVB010000000
VERSION     DAWIVB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14569
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.033"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..128)
                     /locus_tag="WIX60_000731"
     CDS             complement(<1..128)
                     /locus_tag="WIX60_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ5305028.1"
                     /translation="MAFTLMRRAMIRKMPYTEDMRPGDTANPYGASKAMVERMLTD"
     gene            complement(171..515)
                     /locus_tag="WIX60_000732"
     CDS             complement(171..515)
                     /locus_tag="WIX60_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ5305029.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(508..1510)
                     /gene="dcm"
                     /locus_tag="WIX60_000733"
                     /pseudo
     CDS             complement(508..1510)
                     /gene="dcm"
                     /locus_tag="WIX60_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(1541..3814)
                     /locus_tag="WIX60_000734"
     CDS             complement(1541..3814)
                     /locus_tag="WIX60_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ5305030.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVEGKETEGEKFSDYFDHREPVRTMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITRQSEYEQIIACRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALGRLKEAADTDAITVFARNLKDLLLVAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPEMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQNQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(3878..4528)
                     /locus_tag="WIX60_000735"
     CDS             complement(3878..4528)
                     /locus_tag="WIX60_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5305031.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KAFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(4525..5322)
                     /locus_tag="WIX60_000736"
     CDS             complement(4525..5322)
                     /locus_tag="WIX60_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ5305032.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5322..6485)
                     /gene="ctrB"
                     /locus_tag="WIX60_000737"
     CDS             complement(5322..6485)
                     /gene="ctrB"
                     /locus_tag="WIX60_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ5305033.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(6500..7675)
                     /gene="ctrA"
                     /locus_tag="WIX60_000738"
     CDS             complement(6500..7675)
                     /gene="ctrA"
                     /locus_tag="WIX60_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ5305034.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            7802..8935
                     /gene="neuC"
                     /locus_tag="WIX60_000739"
     CDS             7802..8935
                     /gene="neuC"
                     /locus_tag="WIX60_000739"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ5305035.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITLISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELFGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSINVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            8939..9625
                     /gene="neuA"
                     /locus_tag="WIX60_000740"
     CDS             8939..9625
                     /gene="neuA"
                     /locus_tag="WIX60_000740"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ5305036.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGELIAEEAKNFGVEIVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            9626..10675
                     /gene="siaC"
                     /locus_tag="WIX60_000741"
     CDS             9626..10675
                     /gene="siaC"
                     /locus_tag="WIX60_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ5305037.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            10759..12247
                     /locus_tag="WIX60_000742"
                     /pseudo
     CDS             10759..12247
                     /locus_tag="WIX60_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
     gene            complement(12743..13696)
                     /locus_tag="WIX60_000743"
     CDS             complement(12743..13696)
                     /locus_tag="WIX60_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305038.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGCHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            14079..>14569
                     /locus_tag="WIX60_000744"
     CDS             14079..>14569
                     /locus_tag="WIX60_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ5305039.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERI
                     LTD"
BASE COUNT         4293 a         3318 c         2858 g         4100 t
ORIGIN      
        1 atgtcggtta acatccgttc caccatcgct ttggacgcac cataaggatt agcggtatcg
       61 cctgggcgca tatcttccgt atagggcatt ttacgtatca tggcgcgacg cattaaagta
      121 aacgccaaag cgcgtttata ctggttatcc agtttcccta tatttttacg ttacaaatca
      181 agttgttggt attcatcgtg aaaatagaat ttttcggcat aacgctgact cataaaaccg
      241 gcatgcgggc tgcctgaaaa aatcattgcg acatcatcgt catttaatgt ttcatggttg
      301 ttttctatta atgctaaagc aatttggtaa ttaattgcca aaatatcgtt agtaatcaca
      361 cgatagccgc gttttttggc ttcgtaggca aaggaacagc ctccactaaa tacatctgca
      421 actgtatcta catcagacgg aagctggtca caaatccagg aagctatttt ctctttatta
      481 ccgatatagt taattttcgg atattgctta ttcattttca ctcattttta aaatactttc
      541 agcaatagct tttgccaata aaggcggtac ggcgttacca acctgctgct gctgggcaat
      601 tttgctgccg caaaaaataa aattatcagg gaaagattgt aaggcagcta attcacgaac
      661 ggttaacgcc cgattctgtt catagtgaaa aactttgcgc atatctcctg taatacaaac
      721 ggctggtttt gttgctgttg taacggatgt atttacggat atcacctgtt ttcggacgta
      781 atggttcagg aatatcgtta cggttacctc catttttaac aaatgccatt ttttctaaca
      841 tttgtgccga atgattcata gcttcatgat ttgcaacgtg tggattgctt tcgccagcag
      901 ccagttttgg aaaatgtcct attgctgatc caacagtctg atgggaaatc tgcaaaggtt
      961 cgggaaagga aattttgcct ttatccctcc tcccgataaa tatcactcgg ctacgtatct
     1021 gaggaacacc gaaatcggct gcactcagta tcttacattc caccgaataa ccgatattct
     1081 gaaatgcttg aataatctca atacgtgttt tacctgaatt gtgtgtatag agtcgcgcta
     1141 cattttccat aacaaaaaaa tatggttgga caattttaac tattcggaca aactctttaa
     1201 ataaatggtt gcgtgggtca tctgtaaatg tccgtccaat ctttcctgcc atactaaaac
     1261 cttgacaagg tggtcctcca ataatcaaat caactgcttg tccgttaaga caattgatta
     1321 aatcttgttc ggttagtgtg gttaaatctt tttgcagtaa ttgatgatgg gggaagttgg
     1381 tacggtaagt ctgacaataa tcagactcca tttcaacaga aagcaattgt tggaatccgg
     1441 cttgttcaaa acccaaggat aggcctcctg ctcctgagaa aaggtcaata taggtaagtg
     1501 gtgtgtgcat aagtcaaaat ccataaactc ttcgattatt ttaccgcttc agcttcgcaa
     1561 acgcatccgc catcgccgaa ttggctgggg cgcggtcgtt gcgttggggt ttgcggccgg
     1621 ctgtccgttc gcggctgcgg ttttcagacg gcattttatg ttttgcgccg cccggttcgt
     1681 catccaagcg catggtcagc gcgatgcgtt tgcgtgcagc atcgacttcc agcactttca
     1741 ctttcaccac gtcgccagct ttcaccactt cgcgcgggtc ttggacgaac ttgttggaca
     1801 gggcggagat gtgcaccaag ccgtcctgat ggacgccgat gtccacgaac gcgccgaagt
     1861 tggcgacgtt ggaaaccacg ccttcgagta tcataccgac ttgcaagtcg ctgatttcgt
     1921 ggataccttc ggcaaacgat gccgtctgaa actcgccgcg cggatcacgg ccgggttttt
     1981 ccagttcgga caggatgtcc aaaatggtcg gcaggccgaa gcgttcgtcg gtgaagtcgg
     2041 acgctttgat ttgcttcacg cgctcgcggt tgccgatgag ttcggcggcg ctaatgcctt
     2101 gttgcgccag cattttggcg acgacgggat aggcttcggg gtggacggcg ctcgcgtcca
     2161 acggctcttt accgccgtta atccgcaaaa agcctgccgc ctgctcgaag gttttttcgc
     2221 ccaaacgcgg tactttcagc aattttttgc ggctgtcgaa cgcgccgttt tcatcgcggt
     2281 aggcaacgat gttttgggca agggtttgat tcaagccgga aatccgcgcc aagagcgggg
     2341 cggaggcggt attcacgtcc acgccgacgg cgttcacgca gtcttcgacc actgcgtcca
     2401 gcgatttggc gagctggttt tggttcacat cgtgctgata ctggcccacg ccgatggatt
     2461 tagggtcgat tttgaccaac tcggcaagcg ggtcttgcag cctgcgggcg atggacaccg
     2521 cgccgcgcag ggaaacgtcc aagtcgggga actcgcgcgc cgccagttcg gacgcggaat
     2581 aaatcgacgc gccggcttcg gacacgacga ttttgtgcag ccccatttcc ggcattccgc
     2641 gcaccagttc gcccgcgatt ttgtcggttt cgcggctggc ggtgccgttg ccgatggcga
     2701 tgagcttcac gccgtgttgc ttaatcaggc gcgacagcgt tgccaacata ttgttttctt
     2761 gatgcaaata gacgatgacg gtatccagca gcttgccggt gtcgtccacc acggcgcatt
     2821 tcacgccgtt gcggtagccg gggtcgagac ccagcgtggt cagccgtccg gcgggcgcga
     2881 cgagcagcaa gtctttgaga ttgcgggcga acacggtaat cgcgtcggtg tcggcggctt
     2941 ctttcagacg gcctagggct tcaagttcca acgacaaaaa gattttcgcg cgccaagtca
     3001 gacgcacggt atcgcgcagc catttgtggc cgtctgaaac cttgaagcgg caggcgatga
     3061 tttgctcgta ttcgctttgc cgggtaatcg gcgtgtcgtc gggctggtat ttgagcgcga
     3121 tgttcaacac gccttcgttg cggccgcgca aaaccgccag cgcgcggtgg ctgggcatag
     3181 tgcggacggg ttcgcggtgg tcgaaataat cgctgaattt ttcgccttcg gtttctttgc
     3241 cttcaacgac ttgcgcgtgg atttcggctt cgttccacag cttgtcgcgc agcgtgccga
     3301 taagttccgc gtcttcggca aactgctcca tcagaatcgc acgcgcgccg tccaacgcgg
     3361 ctttggcatc ggggacgttt tcgttcaggt agccttgtgc ggcggcttcc acgtcctgcg
     3421 actgctcggc aagcaacacg tccgccagcg gctgcaaacc gtgttcgcgc gcgatttgcg
     3481 ctttggtgcg gcgtttgggt ttgtagggca gatacaggtc ttccagcgcg gttttgttat
     3541 cggcggcttc gatttgcgcc ctgaggtcgt ctgaaagctt gccttgctct tcaatgcttt
     3601 ttaaaacaac ggctttgcgc tcttccaact cgcgcagata ttgcagccgc tcggcaagcc
     3661 ggcgcaactg cgtatcgtcc aacccgcccg tcgcttcctt gcggtagcgg gcgataaacg
     3721 gcacggtcgc gccgtcgtcc aaaagctcga cggcggcggt gatttgcgcg gcagtcgcgg
     3781 agagttcttg ggaaagaatt tgagtaatgt tcatcaatag aattccaacg gacaggccgt
     3841 ctgaaatttc agacggcctg atttaaaaac aatcgcttta aggcagcgaa ttataatatt
     3901 cgtaggcttt gtccatatct tcaaactggt acatatgccc tttttccagc accatcgcat
     3961 tatcgcaata ttgcttcatg gcgctgtggc tgtgcgacac caagatgatg gaacggtctt
     4021 tgcgcttttc aaacaactcg tacttacatt tatcggcaaa acgcgagtca ccaactgcaa
     4081 tcacttcgtc aatcaggtaa cagtcaaact ccaccgccaa cgacagcgca aaagccaaac
     4141 gcgctttcat acctgaagaa tagcgtttca ccggctcata caaatattgc cccagctccg
     4201 aaaattcttc cgtaaacgct ttcacataat cgatatcgac attgtaaatc cggcagatga
     4261 aacgcaaatt gtccataccg gtcagactgc cttgaaacgc accggagaat gccaaaggcc
     4321 aagaaatact cattgtccgc ttgatttcac ccgtggtcgg cggctcaacg ccactgatca
     4381 aacggatgag cgtcgattta cctgcaccgt tgcggccgag aataccgatt ttctcgccct
     4441 tctccatttt gaagctaata tcgtgcaaga ctgtccgcca accttggcgg gtcagatagc
     4501 gtttggaaac gtgttcaact gaaatcattg cggctcgact cctttactga atttactgac
     4561 catcgccaag ccaaacaaca acaacaccag attgcacaat acgatatacc aaggattttc
     4621 ataggtaatt acatcgctgc caaaatatcc ggcacggaac atttctgtgc catgcaccat
     4681 cggaatcatt aatgcatatt cttgtacctt gggcggcaaa ttatgcacaa aaaagaacgc
     4741 accggataac ggcatcatca caaaagtcaa tgtgccccaa atcttgccaa acggctcgaa
     4801 attaaaggca atcgaacaaa tcaccaaacc caaaccaatc gcaaaaaaag ccatcaaaag
     4861 ccaagccatc agcatataaa acatatctgc cggcatttca atccagccaa tcgcaatcaa
     4921 taccgccata atcacaatct gcgcaatggt tgcaccagca atttccaaaa tcatgcgcgc
     4981 caagatggta tccaaaactc ttacattgcg gtgataaagc aagctggcat ttgaagaaat
     5041 cgacccaact gcccgttttg aggcattacg ccacatcatc aacatcggat agccagtaat
     5101 cgcaaatgcg acaatattca aagttgaata tcggtctgcc cttaaaaatt tccacatcaa
     5161 gacgataacg aatgtcatca gcaacggctc aacaaacagc cataaaaagc caatattatt
     5221 gcgaccgtaa cgggtgataa tttcccgcat caacagcgca ccgattacgc gcctttgaat
     5281 ggctaaagat tcccaaaatg atgttttatg caaggctttc atcagttttt atgctcacga
     5341 atgctggcag tcaacaggct caaaatacca taaaccatca agccgataat cagagtggca
     5401 acaatgttgt ataaccgttt aggctcatgt gccaaatccg gcaggctcgg ttgcgagatc
     5461 acttccaaat aaagctgctg acggtctgct tcaaccttgg cactttccaa agaagtcatg
     5521 gcggctgcca actgctgctc tgccaactgg ttttccaaat acacacgctg atattcggca
     5581 gcctgattag acaacgaaga atgcccaccg cccgaaatgg cacgtaactg ttggtcaatt
     5641 tctttacgca agctctgctc acgcgcctgc aaacccggaa tctgcggatt ctccggagtg
     5701 actgctttca cctgatccag ctgggtttga atcacaatca attcatcttg cagcttggaa
     5761 accaacccca tttgcacttc cgattgcgct ttcaaatcaa aaacgccatt ggcaatccgg
     5821 taatccgtca gattctgaga ggcttccttt acccgctctg ccgccgtttt cactacttct
     5881 tccgcatagc gcaccgtatc agcacgtgca cgatcgttca actggttaat caatgcttca
     5941 ccttgtttta acaaagcctc attgattttc ttagattcca gcgcatcaaa ggaagttaca
     6001 ttcaacgtgg aaatacccga aaccgtatca aaattgatca tcacctgatt tttatagtat
     6061 tgataaaaag cctcttcctc gccacggaac ccaaacccat taaagcggct gaacgcatca
     6121 cctttggttt cataaaactc acgcaccggc aagattttac gcagttcatc caaagacgag
     6181 cgcgaacgca tatactcccc aaccgtgtaa atatcatctt gcgcacgggc aaaacctgtg
     6241 ccctgcaaaa tggcacccag gccattgaga gaagattggc ttttaggcga gcgcaccaca
     6301 aagctcgatt gcgacgtaaa acgatcggaa gcgaagaagc cgaaatacac caacgaaatt
     6361 accgtaggga taatcaccgt tacccaaaat aaagggctta gctttttaat ccaacttttc
     6421 tttttcggct ttttacgctc ggctttggtt tcagtggcaa ctgccacagg aagttgctca
     6481 gacatcttaa ttactcacat taattagtta aattattaat actgttcgcg ccactggtaa
     6541 ccggcgagaa cacaaacgac aaaaatttct gcacttcagc caacggcgca ttcgacacat
     6601 acaatacatc tttattcttc acaggaaagc gctgcataga aaatagcgaa tgcgcatcag
     6661 ccatattcac acgatatacc gttggaatct ctgcctcact gccataacct tgagcaatcc
     6721 atttatcctg acgttctgcc ggcaattcca ccaatggcgt atagcggaac acaaacacac
     6781 cacgcgcatc agaacggcga tcttgcaaac cgcccatacg gccaatggct tcagaaagcg
     6841 ataagcctct ggctgaaaaa ccgatttctt gtgttctccc caccgcaccc atagacgtaa
     6901 aggtataggg attggtaatc atggtaacca catcaccgcg acgcagcaaa atattttgtc
     6961 gcggatttgc aactaaatct tccaaggcaa cagttcgtac tacattgcca cgtgtcagct
     7021 gcacattcgt atcctgcaca tttgccgttg aaccacctac cgcagccacc gcatccaaca
     7081 cacgctcacc ggctgccgtc aacggcatac gcacactatt gcctgcgcga atcaccgata
     7141 catttgccgc attattctgc accaagcgca ccatcacttg tggctgattg gccatttttt
     7201 tcaggcggcc tttaataatt tcctgaacct gaccaggcgt tttaccgacc accgaaatat
     7261 cgccaacaaa cggcacagaa accgtaccac gtgccgtgac caactgctct ggcaacttag
     7321 tttgatgcgc actacccgag cccatcgaag aaaggccacc accaaacaat actgccggcg
     7381 gcgcttccca aatcataata tccaatacat caccaatatt tagcgtacca gccgaagcat
     7441 aaccatcgcc aaactgagtg aatgactgat ttatctgagc cttatataat aactgagcaa
     7501 ccgtatgatt cacatcaatc agctccactt caggaatttg aacttcagat tgttgcccta
     7561 aagagacaat tttttttgcg ctggggcctg atgaaggaat cgcagagcat cctacaatta
     7621 aacttccaca caataataat actgcgtgac gaatataaaa tttcacttta aacacaagcc
     7681 aaatcccaat atacttataa atggcctaat tatagcactt aatcgaaata aatttatgag
     7741 tacgtagagt ataattagta ttcttctttc caacttcctt atacttatag attctaaaat
     7801 catgaaaaga attctttgca ttacaggtac cagagccgac ttcggcaagc taaaaccttt
     7861 attagcctat attgaaaatc acccagacct tgaattgcat ttgattgtaa ctggtatgca
     7921 tatgatgaaa acatatggca gaacctacaa ggaagtaact cgagaaaact atcaacatac
     7981 atatctgttt tcaaatcaaa tccaaggtga accaatgggt gccgttttag gcaataccat
     8041 tacgttaatc tctcgtctat ctgatgaaat tgaacctgat atggtcatga ttcacggcga
     8101 ccgtttagaa gcactagcag gcgcaactgt aggtgcatta agcagccgtt tagtttgcca
     8161 tatcgaaggt ggtgaactat ttggtacagt agatgactcc attcgtcatt ctattagtaa
     8221 actttctcat atccacttgg tagcaaatga acaagctgtc actcgcctag tgcaaatggg
     8281 agaaaaaaga aagcatattc acatcatcgg ctcccccgat ttagatgtta tggcctcttc
     8341 caccctccca tccttagaag aagtcaaaga atattacggt ttaccatacg aaaattatgg
     8401 tatttctatg tttcaccccg tgactacaga agcacattta atgccacaat atgcggccca
     8461 atatttcaaa gcattagaat taagtggcca aaatatcatt agcatctacc ctaataatga
     8521 cactggcact gaaagtattc tgcaagaatt attaaaatat caatctgata aatttattgc
     8581 atttccttcc attcgatttg aatatttttt agtcttattg aaacatgcta aatttatggt
     8641 cggcaactca agtgcaggta ttagagaagc tcctctctac ggtgtccctt caattaatgt
     8701 tggtacacgc caaagtaacc gccatatggg aaaatctatt attcatacag attatgaaac
     8761 taaaaatatc tttgatgcga ttcaacaagc atgcagttta ggcaaatttg aagcagatga
     8821 tacctttaat ggcggagata ctcgcaccag cacagaaaga tttgctgaag taatcaacaa
     8881 tcctgaaacg tggaatgttt ctgctcaaaa acgttttatc gatttgaatc tttaaattat
     8941 ggaaaaacaa aatattgcgg ttatacttgc gcgccaaaac tccaaaggat tgccattaaa
     9001 aaatctccgg aaaatgaatg gcatatcatt acttggtcat acaattaatg ctgctatatc
     9061 atcaaagtgt tttgaccgca taattgtttc gactgatggt gagttaattg cagaagaagc
     9121 taaaaatttc ggcgtcgaaa tcgtcctacg ccctgcagag ctggcctccg atacagccag
     9181 ctctatttca ggtgtaatac atgctttaga aacaattggc agtaattccg gcacagtaac
     9241 cctattacaa ccaaccagtc cattacgcac aggggctcat attcgtgaag ctttttctct
     9301 atttgatgag aaaataaaag gatccgttgt ctctgcatgc ccaatggagc atcatccact
     9361 aaaaaccctg cttcaaatca ataatggcga atatgccccc atgcgccatc taagcgattt
     9421 ggagcagcct cgccaacaat tacctcaggc atttaggcct aatggtgcaa tttacattaa
     9481 tgatactgct tcactaattg caaataattg tttttttatc gccccaacca aactttatat
     9541 tatgtctcat caagactcta tcgatattga tactgagctt gatttacaac aggcagaaaa
     9601 cattcttaat cacaaggaaa gctaaatgca aaacaacaac gaatttaaaa ttggtaatcg
     9661 ttcagtaggt tacaaccacg aaccattgat tatctgtgaa atcggcatca atcatgaagg
     9721 ctctttaaaa acagcttttg aaatggttga tgctgcctat aatgcaggcg ctgaagttgt
     9781 taaacatcaa acacacatcg ttgaagacga aatgtctgat gaggccaaac aagtcattcc
     9841 aggcaatgca gatgtctcta tttatgaaat tatggaacgt tgcgccctga atgaagaaga
     9901 tgagattaaa ttaaaagaat acgtagagag taagggtatg atttttatca gtactccttt
     9961 ctctcgtgca gctgctttac gattacaacg tatggatatt ccagcatata aaatcggctc
    10021 tggcgaatgt aataactacc cattaattaa actggtggcc tcttttggta agcctattat
    10081 tctctctacc ggcatgaatt ctattgaaag catcaaaaag tcggtagaaa ttattcgaga
    10141 agcaggggta ccttatgctt tgcttcactg taccaacatc tacccaaccc cttacgaaga
    10201 tgttcgattg ggtggtatga acgatttatc tgaagccttt ccagacgcaa tcattggcct
    10261 gtctgaccat accttagata actatgcttg cttaggagca gtagctttag gcggttcgat
    10321 tttagagcgt cactttactg accgcatgga tcgcccaggt ccggatattg tatgctctat
    10381 gaatccggat acttttaaag agctcaagca aggcgctcat gctttaaaat tggcacgcgg
    10441 cggcaaaaaa gacacgatta tcgcgggaga aaagccaact aaagatttcg cctttgcatc
    10501 tgtcgtagca gataaagaca ttaaaaaagg agaactgttg tccggagata acctatgggt
    10561 taaacgccca ggcaatggag acttcagcgt caacgaatat gaaacattat ttggtaaggt
    10621 cgctgcttgc aatattcgca aaggtgctca aatcaaaaaa actgatattg aataatgctt
    10681 attaacttag ttactttatt aacagaggat tggctattac atatagctaa ttctcattaa
    10741 tttttaagag atacaataat gctaaagaaa ataaaaaaag ctctttttca gcctaaaaag
    10801 ttttttcaag attcaatgtg gttgacaaca tctccatttt atcttacccc cccacgtaac
    10861 aatttatttg tcatatctaa tttaggtcag cttaaccaag tccaaagcct aattaaaata
    10921 caaaaattaa ccaataattt actagtaatt ttatatactt ctaaaaactt aaaaatgcct
    10981 aagttagttc atcaatcagc taacaagaat ctatttgaat ctatttatct atttgagctt
    11041 cctagaagcc ctaataatat aactcctaaa aaattacttt atatttatag aagttacaaa
    11101 aaaatcctta atattataca gcctgctcat ctctatatgc tgtcttttac aggccactac
    11161 tcctatctga ttagtattgc aaaaaaaaaa gaatattacg actcatttaa ttgatgaagg
    11221 gactggaaca tatgctcctt tattagaatc attttcatat catccaacaa aattagaacg
    11281 ttatttgatt ggaaataatc ttaatattaa aggatatata gatcattttg acatattgca
    11341 tgtccccttt cctgaatatg ctaaaaaaat atttaatgca aaaaaatata accggttttt
    11401 tgcgcatgct ggaggaataa gcattaataa taacattgca aacttacaga aaaaatatca
    11461 aatatctaaa aatgactata tttttgttag tcaacgctac cccatttcag atgatttgta
    11521 ttataagagt atagtagaaa tcttaaacag cataagttta caaattaaag gaaagatatt
    11581 tattaaacta cacccaaaag agatgggcaa caactatgta atgtctttat ttctaaatat
    11641 ggtagaaata aaccctcggc tggtagttat taatgaacct ccttttctaa ttgagcccct
    11701 aatatactta acaaatccta aaggaattat aggcctggcc tctagttctt taatttatac
    11761 accattactc tcaccctcaa cccaatgtct ttctattgga gagttaatta ttaacttaat
    11821 tcaaaaatat tcaatggtgg aaaacactga aatgatccaa gaacacttag agattattaa
    11881 gaaatttaat tttattaata tactaaatga tttaaatggg gtaataagta accctctctt
    11941 taaaacagaa gaaacatttg aaacacttct taaatctgca gaattcgcat ataaatctaa
    12001 aaactacttt caggctattt tttactggca acttgccagc aaaaacaata ttaccttatt
    12061 agggcataaa gcattatggt actacaatgc actttataat gtaaaacaaa tttataagat
    12121 ggaatattca gatatttttt atatcgataa tatctccgta gactttcata gtaaagataa
    12181 attgacatgg gaaaaaatta aacattatta ctattccgcc gacaatagaa ttggtagaga
    12241 tagataaaaa tactatgact tctgatctcc atacgtatgc ttgttttcaa aaagcagcat
    12301 taagatagat aagtgaagct tgatgagagc catttttgtg gtcatcgcca aggtacacgc
    12361 ggagagtaat cgacaacaga gctgctgtat tctggctttt aaagcccagt agtaaagttt
    12421 ataaggctgc atttctcaat gtaaaacgcg atattttcac ccattattag ggataagtag
    12481 ccagtggttt acactggcta ctttcatagt tatgaagtac tgggtctgag tgaattcaat
    12541 tatttccgta taaaacacag tagacatttt actaatcggc aaaattatat tagaagaatt
    12601 gagaactact gaagtcaggt aagataccat tttgcacaaa tttaacggca tccctaaaaa
    12661 gcattttggg tggtatttaa aggagtacaa aatggtttta actacagtat gcttagaaat
    12721 ctatagcctt acgcgcttta tattaaatca cctttctcaa ccatttagaa tcataaaaaa
    12781 actttttagg atccctaatt aactttttaa attttcttaa aaaaatagtt ttagttgatt
    12841 ctatactatt cactgcagat aaatattctt ggtaatcatg ttgcaatggc acaacctgct
    12901 ttaatagctt attaaactca tcagttgtaa tcaccttttt agtagcatag caatcaagcc
    12961 aatccggatc tgctattttt ggggaagtga aatctatttg atcaaaataa actaaaaccc
    13021 cactacctaa aaaatgctga tttgcatagg atttagaaaa gcctaagtct gcaacaacac
    13081 caacagggat acccttacat tcagcttcaa aaagcatagt agatgaataa gataaacaat
    13141 accccatttc ttggaaggct tgcgcggtac tttttcgctc tattgtcaaa ttactcggca
    13201 actgaaactg ctttgccagc tctatatacg aatgtttatc ctgatgcaca gtaatatctt
    13261 tatctgcaac ccttagcaaa atagtaaatt ctttctcagg gtatttttca gccaaggcaa
    13321 tcaatttttt taatgtataa attctttctt cttttttaaa tgggatttta acttggtcaa
    13381 taaagtaaat tttctctcca tgacaacctt tcgaagcatg gcgaaaaatt ggataaccat
    13441 aaagtatatt ttgacaactt aatttatatt gtttcttata ttcctctgca attctaaaat
    13501 catgcttatt attatataaa acaatatcag cccccatacg agatagaata cttgcctgat
    13561 caccgaatac tacacctgga aacaaggtaa taatcaatgg ccttgaagca atatttaatt
    13621 gcgcattctg cttaaagaac ctttttaaca acccatttcc aaccgataaa attactgcgt
    13681 cataatattg atgcatattc ttaataaagt aatcatcaat atgaaaaaaa aaattgcttg
    13741 gtctttatct atccctgatt ctaataactg tcgatttgaa agaatgtttt ctcgactttt
    13801 gtggatataa atatcaattt gagcatcttt tatctctttt gcaacagcat agcccgagtt
    13861 aaggaacgag tcataactgg caattagtaa tacttttttc actattttcc cttaattcta
    13921 caccatatga ataaaattat agtattttac cttagatatc aaatatactt aaccaaaaaa
    13981 atctgaagaa atctactgct acttcaggga taattatttc attaagattt tacaaccatt
    14041 attatcaatc tatcaaagta tttttagtat tttattctat gaaaaaaatt ctcgttaccg
    14101 gcggcaccgg ttttatcggc tcgcataccg ttgtttcttt gctgaaaagc ggccatcaag
    14161 tcgtgatttt ggataaccta tgcaattcca gcatcaatat cctgccacgc ttgaaaacga
    14221 ttaccggcca agagattccg ttttatcaag gcgatatccg cgatcgtgag attttgcgcc
    14281 gtatttttgc ggaaaaccgc attgattcgg tgattcattt tgccggcttg aaagcggtgg
    14341 gtgaaagtgt ggccgagccg atgaaatatt atgataataa tgtttccggc agcttggtgt
    14401 tggcggaaga aatggcgcgt gcgggcgtgt ttagcattgt gttcagttct tcggcgacgg
    14461 tttatggcga tccgggcaaa gtgccttata ccgaggatat gccaccgggc gacaccacca
    14521 gcccttacgg cgcatcgaaa tcgatggttg agcgcattct cactgacat
//
LOCUS       DAWIVB010000034        13694 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWIVB010000034 DAWIVB010000000
VERSION     DAWIVB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.034"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(122..1447)
                     /locus_tag="WIX60_000745"
     CDS             complement(122..1447)
                     /locus_tag="WIX60_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ5305040.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1658..2497
                     /locus_tag="WIX60_000746"
     CDS             1658..2497
                     /locus_tag="WIX60_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ5305041.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGETLVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2494..3660
                     /locus_tag="WIX60_000747"
     CDS             2494..3660
                     /locus_tag="WIX60_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ5305042.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3924..5189
                     /gene="efeB"
                     /locus_tag="WIX60_000748"
     CDS             3924..5189
                     /gene="efeB"
                     /locus_tag="WIX60_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ5305043.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYNYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5259..5588)
                     /locus_tag="WIX60_000749"
     CDS             complement(5259..5588)
                     /locus_tag="WIX60_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ5305044.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQKGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5665..7035)
                     /gene="glmU"
                     /locus_tag="WIX60_000750"
     CDS             complement(5665..7035)
                     /gene="glmU"
                     /locus_tag="WIX60_000750"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ5305045.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMVGRVIDTAA
                     ALNPQNICVVIGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVETLETLLEAAGDEVGLLTDVPHDPAGLGRIIRDSNGNVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SYLAAGVNNKLQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGDIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     ARLSDDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            complement(7090..7362)
                     /locus_tag="WIX60_000751"
     CDS             complement(7090..7362)
                     /locus_tag="WIX60_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ5305046.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPDQTDGSEQGVRGQNWQKPKFQNR"
     gene            complement(7355..8068)
                     /locus_tag="WIX60_000752"
     CDS             complement(7355..8068)
                     /locus_tag="WIX60_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ5305047.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQEYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTKLHGMADTEHTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLAQYAETLSEH
                     RQNHYNTRTTPENTKECYA"
     gene            8127..9128
                     /locus_tag="WIX60_000753"
     CDS             8127..9128
                     /locus_tag="WIX60_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5305048.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGMGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9222..10070
                     /locus_tag="WIX60_000754"
     CDS             9222..10070
                     /locus_tag="WIX60_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ5305049.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10097..10582
                     /locus_tag="WIX60_000755"
     CDS             10097..10582
                     /locus_tag="WIX60_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ5305050.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDCLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10684..12252)
                     /gene="msrAB"
                     /locus_tag="WIX60_000756"
     CDS             complement(10684..12252)
                     /gene="msrAB"
                     /locus_tag="WIX60_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ5305051.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12397..13662
                     /gene="ftsY"
                     /locus_tag="WIX60_000757"
     CDS             12397..13662
                     /gene="ftsY"
                     /locus_tag="WIX60_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ5305052.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2921 a         3518 c         4121 g         3134 t
ORIGIN      
        1 aatttgatcc actataattc cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa
       61 aacttcgcct taatgcgccc gattgcctag ggatgggctt cagatggcat tgttttccgg
      121 tttacgggcg gtattcgggc ttcataccgt tgggtaggag ctgccagaca tatcccgtgg
      181 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta tccccaaaaa taatccacgc
      241 gcaccgcgcc tttaatcgcg ctgccggtat cctgcgccat aatcaggcgg ttgagggctt
      301 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc acccaaggta atgtagtgcc
      361 ggtcgactgc gccggcatat tcccccatca gcggcgtgcc cagtgcgccg acagggccgt
      421 cattgctgct tccggcaagc tcgcggaaaa agatatagct ggggttttga cccaaaactt
      481 cggcgaggcg ttgcggattt tgccgcatat aagacttaat gccctgcatg gaggtttgtc
      541 cgagtttgag gtagccctta tccgccatat agcgtccgat ggaaacgtag ggatgttcgt
      601 ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga cggcccgagc
      661 cttggatgtg cataaaaaaa agttcgacag ggtcttcggc gtaaccgagt atcggggctt
      721 tgccgtcaag cgcgccgccg ttgatttggt tgcgcgtgtg gtaggggagg aagcggcttc
      781 cttcaaacct gcctttgatt gctgttgtgc gcgcggtgat ggggaatcgg gagaggtcgg
      841 cggtatgtgt gccgccggta ttgtcgattg tgccgctgtt ttttcccgtc tgcctgatgc
      901 ggacaagggc ttttccgctc cgcaaaccgg caggcagggg gacggagata aaatcgtcgg
      961 gaataccgta aatcgggaag cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg
     1021 gttcgtaata gccggtaacc gtaccggcaa ggcttccgtt gcctgcaacc tgccacggcg
     1081 tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt
     1141 gggcgcacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     1201 attgcaggct tttggcgaaa tcctgcgccg cccagtgggg cagggacagg tgcggtacaa
     1261 cggtatagac ggccccgccg ccgccgaccg tcgttccggc ggggtcgggg atgccgaccg
     1321 gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg atgctcttgc
     1381 tttggcaggc ggcgaggatg gcggcggcga tgccgtacag ggcggcgcgg aataggtatt
     1441 ttttcataat ggacaatgtt gccggcagta ataagaaaga tggtttcggg cggcgttgcg
     1501 gcagccgtgg agaggggatt ttaacacagg gcgcagctgc agcctgcgga actttccgcc
     1561 gcgcggtact gcagataaaa ataacttgca tttgtattta caagcaatga aaatattccg
     1621 ataatatatt attcatcatc cttgttcgtt cgcgtttatg ctggtcgctt ttttaattat
     1681 gttgcgcgag ggtattgaag ccgcgctcat tgtcggcatc gttgccggtt ttctgaaaca
     1741 gtccggacat tccaaactga tgcctaaggt ctggttcggg gtcgtccttg cttctttgat
     1801 gtgtttgggg ctggggtacg gcatccattc ggcaacgggc gagattcccc agaagcagca
     1861 ggagttcgtc gtcggcatta tcggtttggt tgccgttgcc atgctgactt atatgatttt
     1921 atggatgaaa aaggcggcgc gttcgatgaa gcggcagctt caggattctg tgcaggcggc
     1981 tttgaaccgt ggcagcggtc aaggatgggc cttggtcggt atggcgtttc ttgccgtggc
     2041 gcgcgaaggt ctggagagtg tttttttcct gcttgccgta ttcaaacaga gcccgacgtg
     2101 gcagatgccg gccggcgcgg tagcgggggt tttggctgcc gccgtgattg gcgcgttgat
     2161 ttatcagggc gggatgcgcc tgaatctggc gaagtttttc cgttggacgg gggcgtttct
     2221 gattgtcgtt gccgccggcc tgcttgccgg ctcgctgcgc gcgctgcatg aggcaggtat
     2281 ttggaacgcg cttcaggaca ttgtgttcga ctcatcaaaa tatttgcacg aagacagtcc
     2341 gttgggcgtg ctgctcggcg gatttttcgg ctataccgac catccgacgc agggcgagac
     2401 cttggtttgg ctgctgtacc ttattcccgt cataacttgg tttttgtgcg gcagcaggcc
     2461 gtctgaaact ttaacccgta aagaggagct gaaatgagaa aattcaattt gaccgcattg
     2521 tccgtgatgc ttgccttagg tttgaccgcg tgccagccgc cggaggcgga gaaagctgcg
     2581 ccggcagcgt ccggtgaggc gcaaaccgcc aacgagggcg gttcggtcag tatcgccgtc
     2641 aacgacaatg cctgcgaacc gatggaactg accgtgccga gcggacaggt tgtgttcaat
     2701 attaaaaaca acagcggccg caagctcgaa tgggaaatcc tgaaaggcgt gatggtggtg
     2761 gacgagcgcg aaaacatcgc ccccggactt tccgataaaa tgaccgtcac cctgttgccg
     2821 ggcgaatacg aaatgacttg cggtcttttg accaatccgc gcggcaagct ggtggtaacc
     2881 gacagcggct ttaaagacac cgccaacgaa gcggatttgg aaaaactgtc ccaaccgctc
     2941 gccgactata aagcctacgt tcaaggcgag gttaaagagc tggtggcgaa aaccaaaact
     3001 tttaccgaag ccgtcaaagc aggcgacatt gaaaaggcga aatccctgtt tgccgacacc
     3061 cgcgtccatt acgaacgcat cgaaccgatt gccgagcttt tcagcgaact cgaccccgtc
     3121 atcgatgcgc gtgaagacga cttcaaagac ggcgcgaaag atgccggatt taccggcttt
     3181 caccgtatcg aatacgccct ttgggtggaa aaagacgtgt ccggcgtgaa ggaaattgca
     3241 gcgaaactga tgaccgatgt cgaagccctg caaaaagaaa tcgacgcatt ggcgtttcct
     3301 ccgggcaagg tggtcggcgg cgcgtccgaa ctgattgaag aagtggcggg cagtaaaatc
     3361 agcggcgaag aagaccggta cagccacacc gatttgagcg acttccaagc caatgtggac
     3421 ggatctaaaa aaatcgtcga tttgttccgt ccgctgatcg aggccaaaaa caaagccttg
     3481 ttggaaaaaa ccgataccaa cttcaaacag gtcaacgaaa ttctggcgaa ataccggact
     3541 aaagacggtt ttgaaaccta cgacaagctg ggcgaagccg accgcaaagc gttacaggcc
     3601 tctattaacg cgcttgccga agaccttgcc caacttcgcg gcatactcgg cttgaaataa
     3661 gccgcaagcg ttcagacggt atttggcggc agataccgtc tgaagtttta tagtggatta
     3721 acaaaaacca gtacggcatt gcctcgcctt gccttgccgt actatttata ctgtctgcgg
     3781 cttcgtcgcc ttgtcctgat ttttgttaat ccactatatc cgccatatat tgcagggcgg
     3841 gatttcaacc tgccgctatc ggttaatgga aaaacggcgt gcagggatac ccatcctgct
     3901 gcacggatat tgaaggaaac accatgagca aaaaccaacc cgcacaaccg accaggcgca
     3961 ctctttttaa aaccgcgatc gcagccggcg cagtcggcgc aatcggaggt tatctcggcg
     4021 gcaaaaaaca gggcgaaacc gccgaacgca ccgccgaaag ccaacactcg ccccaagcct
     4081 atccctgcta cggcgaacat caggcaggca tcgttacgcc gcagcaggcg ttttcgatta
     4141 tgtgcgcctt cgacgtaacc gcgcaaagtg ccaagcagct ggaaaacctg ttccgcacgc
     4201 tgaccgcccg catcgagttt ctcacccaag gcggcgaata ccaagacggc gacgacaaac
     4261 ttccgccagc cggcagcggc attttgggca aagccttcaa ccccgacggg ttgaccgtta
     4321 ccgtgggggt gggcagcagc ctgtttgacg gccggttcgg actcaaagac aaaaaaccga
     4381 ttcatttgca ggaaatgcgc gacttctcca acgataagct gcaaaaaagc tggtgcgacg
     4441 gcgatttgag cctgcaaatc tgtgccttca cccccgaaac ctgccaagcc gccctgcgcg
     4501 acatcatcaa acacaccgtc caaaccgccg ttatccgctg gagtatcgac gggtggcagc
     4561 ctaaatccga acccggcgcg atggcggcgc gcaacctgtt gggcttccgc gacggcacgg
     4621 gcaaccccaa agtttccgac cccaaaactg ccgacgaggt tttgtggacg ggggtggccg
     4681 ccaacagcct cgacgaaccg gagtgggcga aaaacggcag ctatcaggca gtccgcctta
     4741 tccgccactt tgttgagttt tgggacagga cgccgcttca agagcaaacc gacattttcg
     4801 ggcggcgcaa atacagcggc gcgccgatgg acggcaaaaa agaagccgac caaccggatt
     4861 ttgccaaaga ccccgagggg aataccacgc ccaaagacag ccatatacgc ctggcgaatc
     4921 cgcgcgatcc cgagttcctt aaaaaacacc gcctcttccg ccgcgcctac aactattcgc
     4981 gcggactcgc ctcaagcgga cagcttgatg tcgggctggt gttcgtctgt tatcaggcaa
     5041 accttgcaga cggcttcatc ttcgtgcaaa acctcctcaa cggcgaaccg ctggaagaat
     5101 acatcagccc cttcggcggc ggctatttct tcgtgctgcc cggcgtggaa aaaggcggct
     5161 tcttgggaca agggctgctg ggcgtataaa cctgcctgac ggacaaatgc cgtctgaatc
     5221 cgataagggg ttcagacggc attttagtgt ttgggcggtc aggctttttt gacgaattca
     5281 gattttaaat tcatcgcgct gccgtcgatt ttacagccga tgttgtgatc gcctttctgc
     5341 aggcgtatgc ctttgacctt cgtgccttgt ttgatcacca tcgagctgcc ttttaccttg
     5401 aggtctttga tgaggatgac ggtgtcgccg ttttgcagtg ccgcgccgtt ggcatcgcgc
     5461 acttgagccg caaggtcggc ggcggattcg gtttcattcc attcgtgggc gcattcgggg
     5521 cagatgtatt gtccgccgtc ttcataagtg tattcggagg cgcattgtgg gcatgggggt
     5581 aatgacatgg tttgcagtcc ttatctgata tcggaggagt ttgatgccgt ctgaaacctg
     5641 aaaccggctt cagacggcat agctttattg cttgtctttt tcaggccgca tccagccttc
     5701 gatgacggtt tggcgggcgc gggcgagggc gagtttgttg tcttcgatat tgcgggtaat
     5761 cgtgctgcct gcgcctgtgg ttactttgtt gccgagggta acgggggcga ctaggacgca
     5821 gtttgaaccg atgcggactt cgtcgccgat aacggttttg tgtttgtgca caccgtcgta
     5881 gttggcaata atcgtacccg cgccgaagtt ggttttgctg ccgacttcgg catcgccgat
     5941 gtaggtgagg tggttggctt tggtgccttt gccgatggcg gcgtttttga tttcgacgaa
     6001 gttgccgacg tgtacgtcgt ctgaaaggcg ggcttgcgga cgcaggcggg cgtacgggcc
     6061 gattcggttg ttttcgccga cttcgcagct ttcgaggtgg gagaaggggg cgattttgct
     6121 gtttgcgccg attttggcgt ttttgatgac gcagtttgcg ccgatttcga cgttgtcgcc
     6181 gagctcgatg tcgccttcaa agatacagtt cacatcaatc acgacgtctt gcccgtgttt
     6241 cagacggcct cgtaaatcga aacgtgccgg atcgcgcagg gttacgcctg ctttgagcaa
     6301 ttcttgcgcc tgttcggttt ggaagatgcg ttcgagttcg gcgagctgga gtttgttgtt
     6361 cacgccggcg gcgaggtagg aggcgcgcac ttggacggga tggactttaa taccatcggc
     6421 aacggctttg gcgatgaggt cggtcagata gtattcgcct tgtgcgttgt tgctggagag
     6481 gctgttcagc cagttttcca gtttggcgtt gggcaggacg aggatgcctg tgttgatttc
     6541 tttcacggct ttttgggcgg catcggcgtc tttttcttcg acaatggcgg ttacgttgcc
     6601 gttgctgtcg cggatgatgc gccccaagcc tgccgggtcg tggggcacgt cggtcaacag
     6661 gccgacttcg tcgcctgcgg cttcgagcag ggtttcgagg gtttcaacgt caattaaagg
     6721 aacgtcgccg tacaacacca gcgtgcggcc ttcggcggaa aggtggggca gggcggtttt
     6781 gacggcgtgg ccggtaccga gctgttcggt ttgttcaacc caaacgacat cgcgtttgac
     6841 ggtgtccaag acttgctctt tgccgtggcc gatgacgacg cagatgtttt gcggattcag
     6901 tgcggctgcg gtgtcgataa cgcgcccgac catgggcttg ccgccgatgc ggtgcagcac
     6961 ttttggcatt ttggaataca tgcgcgtgcc tttgccggcg gcgaggatga cgatgtttaa
     7021 agtgttttgc ggcatgacgg tttcctgtgc gatgccgtct gaagcggctt cagacggcat
     7081 agggtgggtt tatcggtttt gaaatttggg cttttgccag ttctgtccgc gtacgccttg
     7141 ttcggaaccg tcggtttggt cgggcagcac ggcatggcgt tcggggcggt attggttgta
     7201 gttcatattt ttcgagtagc tgccgtcttg gtaataaacg ggcgtgccgg cgggatattt
     7261 ttgacggacg gcggtcttgc cgttgccgtc ttgataagtt tcccacgcgc agcccgacaa
     7321 aagggcggcg gcagtcatca ggataaggaa agttttacgc ataacattct ttcgtatttt
     7381 cgggagttgt gcgggtattg taatgatttt ggcggtgttc tgacaaagtt tctgcatact
     7441 gtgccagttg cgccatatcg cttacggaag catcgataaa gggcaggtcg tgcacttttg
     7501 cgccgaaccg gacggttttc atgcccaatg cttttgcctg atgcaggttg tccgcgctgt
     7561 cgtccaccat aatgcagcat tcgggcggta cgtccaaaag gcggcagaca ttgagatacg
     7621 cttgcggatt gggtttgtac agcagtccga aatcatccgt gccgaacagt gcgtcgaaac
     7681 ggttttccaa accgagtgcg ccgacaacgg cacggacgta aaacgacgga ccgttggaaa
     7741 aaaccgcctt gcgcccgttc agacggcata gggtgtgttc cgtatccgcc atgccgtgca
     7801 gcttggtcag gattgcatcg atcggatggc tttcgcgtaa aaattcggcg atgtcgattt
     7861 cgggatggtg gatttgcagt ccggcgagcg ttgcgccgta gcggtgccaa tattcctgac
     7921 gcaggtcgga cgcggcggat tcggagagtt tgaggcggcg tgccatatag cgtgtcatag
     7981 cgcggttgat gagtgtgaag atgcctgcgt cggcatcgtg cagcgtgttg tcgaggtcga
     8041 acagccacac ggtcgggttt tcttgcattt tgaaccgtga aaatttgtta gaatgttatt
     8101 ttacagcgaa tagaggagga ctcggaatga aacggaaaat ttggctgctg ccgctgctgg
     8161 cggtttcggc atacctgcag gcgcagacgg aagtcaggct ggcggtgcat aagtcgttca
     8221 gcctgcccaa agggctgatt gcgcgcttcg agcgggcaaa cgatgcgaag gtgtcgatta
     8281 ttcaggcggg cggcgcgaac gaaatgctca acaaactgat tttgagccgc gccaacccga
     8341 tcgccgacgc ggtgtacggc ttggacaacg ccaatatcgg caaggcgcgg gaaatgggca
     8401 ttttggcggc ggcgcaaccc gaatccgccc ccgtcgcggt cgggctgcct tcggctttgg
     8461 cggtcgatta cggctatgtg tccatcaatt acgacaaaaa atggtttgaa ggcaaaaagc
     8521 tgcccctgcc gcaaaccctg caggatttga cccgccccga atataaaaac ctattggtcg
     8581 tgccgtcccc cgccacgtcg tccccggggc tgggcttcct gatggcgaac atcagcggga
     8641 tgggcgaaga aggcgcgttc aaatggtggg cacagatgcg gcagaacggc gtgaaggtcg
     8701 ccaaaggctg gagcgaggcg tattacaccg acttttcgca caacggcggc gcgtatccgc
     8761 tggtggtcgg ttatgccgcc agtccggcgg cggaagtgta tttttccaaa ggcaaataca
     8821 gcgagccgcc gacgggcaac ctgtttttaa aaggcggcgt attccgccag gtcgaaggcg
     8881 cggcagtctt gaagggcgcg aaacagccga agctggcggc aaaactggtg caatggctgc
     8941 aaagtcggga agtgcagcag gcggttccgt ccgaaatgtg ggtttacccc gccgtcaaaa
     9001 acacgcgcct gcccgacgtg ttccgcttcg cccaagcccc gacgcacacc accgcccccg
     9061 cgcagcgcga tattgatgcg aaccagcgcg gatgggtttc ccgttggatt agaacggttt
     9121 tgaaataaaa caaacatacc gccccgcagg gcttcatacg gcatttttac acctgtgccg
     9181 attacgccgc acggggcgga tgttcgatca agaggaaaac aatggacttc aaacaatttg
     9241 attttttaca cctgatcagt gtttccggtt gggagcatct ggctgaaaag gcgtgggcgt
     9301 tcgggctgaa ccttgccgcc gcgctgctta tttttttggt cggaaaatgg gcggcgaaac
     9361 gcattgtcgc tgtgatgagg gcggcgatga cgcgcgcgca ggtcgatgcc acgctgatta
     9421 gttttttgtg taatgttgcc aatatcggct tattgatttt ggtgattatt gccgcattgg
     9481 gcagattggg cgtttccaca acatccgtaa ccgccttaat cggcggcgcg ggtttggcgg
     9541 tggcgttgtc cttgaaagac cagctgtcca attttgccgc cggcgcgctg attatcctgt
     9601 tccgcccgtt caaagtcggc gattttatcc gcgtcggcgg ttttgaagga tatgtccgag
     9661 agattaaaat ggtgcagact tctttgcgga cgaccgacaa cgaagaagtc gtgctgccca
     9721 acagcgtggt gatgggcaac agcatcgtca accgttccac cctgccgctt tgccgcgccc
     9781 aagtgatagt cggcgtcgat tacaactgcg atttgaaagt ggcgaaagag gcggtgttga
     9841 aagccgccgt cgaacacccc ttgagcgttc aaaacgaaga gcggcaggcc gccgcctaca
     9901 tcaccgcctt gggcgacaat gccatcgaaa tcacattatg ggcttgggca aacgaagcag
     9961 accgctggac gctgcaatgc gatttgaacg aacaagtggt cgaaaacctc cgcaaagtca
    10021 atatcaacat cccgttcccg caacgcgaca tacacatcat caattcttaa acgccgtctg
    10081 aaaggggagt gggaaaatgg acgcgctgca caccatcgcc cgaaacctga cgaaaaaacg
    10141 tcaaaccgta agctgtgccg agtcctgtac gggcggaatg cttgccgccg cattcacaag
    10201 cgttgcaggc agttcgcaat ggttcgacca gagttttgta acatacagca acaaagccaa
    10261 agaagactgc ttgggcgtgt tgcccgaaac cctgctcgaa cacggcgcgg tcagccgcca
    10321 aaccgtctat gagatggcgc gcggcgcgaa agccgtggcg caggcggatt acgccgtcgg
    10381 tatttccggc atcgccggac cgggcggcgg cagcgaaagc aaacccgtcg gcacggtttg
    10441 gttcgggttt gcctttccgg gcggaagttg cgaagcaatg cgccgttttg acggcaaccg
    10501 cgaatccgtc cgcgcgcagg cggtcgcctt cgcgttggaa cggttggcgg ggctgattga
    10561 aaacggcggc gatgctgtct aaacaaaatc tccgtctgga cagaatcccc gccggataaa
    10621 aaataccgtc tgaaacgttg caggtttcag acggtatttt gtcgggttag gcggcggtgc
    10681 ggcttatttc actttactct tcaacgcgcc gtagcctgcc gcgtccattt gttccagcgg
    10741 gatgaatttc aagctcgcgc cgttgatgca gtagcgcagt ccgcctttgt cgcgcgggcc
    10801 gtcggggaag acgtgtccca aatgcgaatc ggcggcgtgg ctgcgcactt cggtgcggcg
    10861 catgttgtag ctgaaatcat cgtgttcggt aacggatttt gcatcaatcg ggcgcgtgaa
    10921 gctcggccag ccgcagccgg aatcatattt gtcggcggag ctgaacaaag gttcgccgct
    10981 gacaacgtcc acataaatgc cgggtttgaa caaatggtcg tattcgtggc tgaaggcata
    11041 ttcggtcgcg ctgttttggg taacttggta ttgctcttcg gtcagggtgc gtttgagttc
    11101 ggcgtcactc ggttttttat acgttgccgc atcgaagcct ttgccttgcg gggcggtctt
    11161 ggttttgccc ggcagcggtt cgtcggcttt gcggatatcg atgtggcagt agccgttggg
    11221 gtttttaatc aggtagtcct gatggtattc ctcggcatcg tagaagtttt tcagcggctc
    11281 gttttcaaca acgaggggca gttggtattt ttgctgctcg cgtttgaggg cggcggcgat
    11341 gacggctttt tcggcggggt cggtgtagta cacgccgctg cggtattgcg taccggtgtc
    11401 gttgccctgt ttgttgaggc tggtcggatc aacgacgcgg aagaaatatt gcaggatgtc
    11461 gtctaggctg agtttgtcgg catcgtaggt cactttgacg gtttcggcgt ggcccgtatg
    11521 gcggtaggac acgtcttcat agctcggatt tttcgtgttg ccgttggcgt agccggatac
    11581 cgcgtcaacc acgccgtcga tgcgttggaa ataggcttcc aagccccaga agcagccgcc
    11641 ggcgaggtag atggtgcgcg tgttcatgat ttttgaatcc tttttctgag tgtcgggttt
    11701 gtagaacgaa tgtttcaagc tgcccaaatc ggcattcggg tcgcggatta acgccaacgc
    11761 ctgcgcttcg ttgatgctgc ctttgacgat gcgctgcacg tcgccgtctt taccgattaa
    11821 cgcccacgaa gggtaaacgc tgatattcag gctttgggcg atcgtgccgc cgttgtcggt
    11881 tacgacgggc agcttgggat aattcaaacc ggcataccat ttttggaagt cgccgtcttt
    11941 tttctcgtgc aaaaagcccg gagacgcgac ggtaatcagg ttggcggagc tgaattttgc
    12001 atcttgcgcc catttttcgg tctgtcccaa ttcggacaga cacaaaggac accagctcgc
    12061 ccaaaattta atcagcgtcg gtttgtcttt tttcaagtaa acactggcgg ggcggttgtc
    12121 cgcagttttc aaagtggata aagtgtgcgg cacggtcgcg gctccggcat cgacgatttt
    12181 gggcgaacaa gcacccagcg caagcaggca gccgaacttg gcgcaaaggg aaaagaaagt
    12241 acggtgtttc attttgagtt tcctgtgtgg acggtttgca tgattagacg tttgagatgc
    12301 cgaaacctta cagctcggat tttcagacaa ccttgccgcg taaaatacgc tacaatacgc
    12361 cctgtttcaa gtttctaaaa ttaaaaggaa aattcaatgt tcagcttctt ccgtcgcaag
    12421 aaaaaacagg aaacgccggc tcccgaggag gctcaagttc aggaaactgc agcaaaagca
    12481 gaatccgaag ttactcaaat agttgaaaat attaaagaag aagtcgaatc tttaacggaa
    12541 agcgtcaaag ggcaggtcga atcggctgtt gaaaccgtca gcggtgcggt tgaacaagta
    12601 aaggaagccg ttgccgagat gctgtctgaa gcagaggaag cggcggaaaa agcagcggaa
    12661 caagtcgaag cggcaaaaga agccattgcc gaaaccgtcg gcgaggctgt cgggcaagtt
    12721 caagaagccg ttgcaacaac tgaagaacac aagctcggtt gggcggcgcg tttgaaacaa
    12781 ggtttgacca aatcgcgcga caaaatggcg aaatcgctgg cgggcgtgtt cggcggcgga
    12841 caaatcgacg aagatttgta cgaagagctg gaaaccgtgc tgattaccag cgatatgggc
    12901 atggaagcca ccgaatacct gatgaaagac gtgcgcgacc gcgtcagcct caaagggctg
    12961 aaagacggca acgaattgcg cggcgcgctg aaagaagcct tgtacgacct gattaagcct
    13021 ctagagaaac cgctggtttt gcccgaaacc aaagagccgt ttgtcatcat gcttgctggc
    13081 atcaacggcg cgggcaaaac cacatccatc ggcaaactcg ccaaatattt ccaagcgcag
    13141 ggcaaatctg tgctgcttgc cgccggcgat actttccgcg ccgccgcgcg tgagcagctt
    13201 caagcttggg gcgaacgcaa caacgtaacc gtgatttcgc aaaccacggg cgattccgcc
    13261 gccgtgtgct tcgatgccgt ccaagccgcc aaagcgcgcg gcatcgacat tgtgctggcc
    13321 gacaccgccg gccgcctgcc cacgcagctt cacttgatgg aagaaatcaa aaaagtgaaa
    13381 cgcgtgctgc aaaaagccat gcccgacgcg ccgcacgaaa tcatcgtcgt gcttgatgcc
    13441 aatatcggtc aaaacgccgt caaccaagtc aaagcctttg acgacgcatt agggctgacc
    13501 ggtttaatcg tgaccaaact cgacggcacg gcaaaaggcg gcatcctcgc cgcgcttgcc
    13561 tccgaccgcc ccgtccccgt ccgctatatc ggcgtgggcg aaggcataga cgacctgcgc
    13621 ccgtttgacg cgcgcgcgtt tgtggacgca ctgctggatt gagccgaaat gccgtccgaa
    13681 aacagcagac cgat
//
LOCUS       DAWIVB010000035        13430 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWIVB010000035 DAWIVB010000000
VERSION     DAWIVB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.035"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(123..458)
                     /locus_tag="WIX60_000758"
     CDS             complement(123..458)
                     /locus_tag="WIX60_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ5305053.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(538..2652)
                     /gene="dnaX"
                     /locus_tag="WIX60_000759"
     CDS             complement(538..2652)
                     /gene="dnaX"
                     /locus_tag="WIX60_000759"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ5305054.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELKSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAEPVTNQENNDIPPWEDAPDETAAGTAQA
                     SAKSIQTASEAGTPPKNQVSKNEAADNETDAPLSEVPSENPIQATPNNEALETEAFAH
                     EAPAKPFNGYSFPNDDYLVEDGAEIPPPDWEHAAPADAEEENNADESSNNEDHTPYAP
                     PPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATADKKR
                     LDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADPAAQ
                     KILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1424..1463)
                     /gene="dnaX"
                     /locus_tag="WIX60_000759"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2747..4723)
                     /gene="mutL"
                     /locus_tag="WIX60_000760"
     CDS             complement(2747..4723)
                     /gene="mutL"
                     /locus_tag="WIX60_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ5305055.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAIE
                     VELAGGGIRLIRVSDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQNDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGEDFQT
                     ASLGIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSVSNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4867..5052
                     /locus_tag="WIX60_000761"
     CDS             4867..5052
                     /locus_tag="WIX60_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ5305056.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5063..5731
                     /locus_tag="WIX60_000762"
     CDS             5063..5731
                     /locus_tag="WIX60_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ5305057.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            5731..6444
                     /locus_tag="WIX60_000763"
     CDS             5731..6444
                     /locus_tag="WIX60_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ5305058.1"
                     /translation="MKIKLPLFIIWLSVSASCASVSPVPAGSQTEMSTRENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHSSGRAYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYKSGKFSAAASLLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            6492..6977
                     /gene="purE"
                     /locus_tag="WIX60_000764"
     CDS             6492..6977
                     /gene="purE"
                     /locus_tag="WIX60_000764"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ5305059.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLNMELEQ
                     I"
     gene            complement(7160..8614)
                     /locus_tag="WIX60_000765"
     CDS             complement(7160..8614)
                     /locus_tag="WIX60_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ5305060.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8611..9312)
                     /locus_tag="WIX60_000766"
     CDS             complement(8611..9312)
                     /locus_tag="WIX60_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ5305061.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9309..10088)
                     /locus_tag="WIX60_000767"
     CDS             complement(9309..10088)
                     /locus_tag="WIX60_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ5305062.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10373..11779
                     /locus_tag="WIX60_000768"
     CDS             10373..11779
                     /locus_tag="WIX60_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ5305063.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLDMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGTNLTRNTPS"
     gene            11891..13417
                     /locus_tag="WIX60_000769"
     CDS             11891..13417
                     /locus_tag="WIX60_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ5305064.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGDIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     CIDWQSVRTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPAYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
BASE COUNT         2709 a         3616 c         3785 g         3320 t
ORIGIN      
        1 tgatgccgtc attcccgcga aggcgggaat ccagacccct cggcacggaa atttatcgag
       61 taaaacggtt tcttagattc tacgttctag attcccgcct gcgtgggaat gacggtcggg
      121 gatcagcgga agaagtcgcc cactccgggg ggtagacctt gcgtgaatgc gcccattgtt
      181 ttgtttgcgg tttcttcggc tttgcctcgg gcggatttga gggcggcgag gatgaggtct
      241 tcaagcattt ctttgtcgtc ggcggcttct tgaatcaaat cggggctgat gtcgattttg
      301 cgtacttcgt gcgcgcaggt cattgtgatt ttgaccaggc cgttgcctgc ttcgccttcg
      361 atttcggttt cggcgagttt ggcttgcgct tttttcatat tttcctgcat ttgctgcgcc
      421 tgtttcatca ggccgcctaa tccggctttt ccgaacatac tgaatactcc tgtctgtttt
      481 gattgaataa gggaaacggc aatgccgtcc gaagggttcg ggcggcatta tatctgttta
      541 tggccggttt gccgccaatt ccagtgattc gggctgccat tgcgcgccga atgcttggag
      601 gattttttgt gcggcggggt cggcttcgag caatgcttgt gctttttgcc tgtcttcggc
      661 ttggacgcgc ttgtcctgca tcgcgggggt ttcccggccg gcttcgtcac gccagtcttg
      721 ggtttgcagg gtgagttgca gcccgtaggc ttgggcaagg gtgtcgcgga ttttgtcgag
      781 gcgttttttg tcggcggtgg cgcgtgcttc ggcggtcatt gccaaaacca tcagaccggt
      841 gtcgggatgg tattccgtcc acgcggagtg ttgcgccggc atttgcgccg cgccgagttt
      901 gcgggcgaag tgccggacga tggctgccca gttttcggtg gaaaattcgg gcggcggggc
      961 gtatggcgtg tggtcttcgt tgttgctgct ttcgtcggcg ttgttttctt cttccgcatc
     1021 ggcaggggcg gcgtgttccc aatcgggcgg tgggatttct gcgccgtctt ctacgaggta
     1081 gtcatcattc ggaaagctgt aaccgttgaa aggttttgca ggagcttcgt gtgcaaatgc
     1141 ttctgtttca agggcttcat tattcggtgt tgcctgaatg gggttttcag acggcacttc
     1201 ggacaagggg gcatcggttt cgttgtcggc tgcttcgttc ttggaaactt ggtttttggg
     1261 cggcgttccg gcttcggatg ccgtctgaat gctttttgcc gatgcttgcg ccgtgccggc
     1321 tgcggtttcg tccggcgcgt cttcccaagg cggaatatcg ttgttttctt gattggtaac
     1381 gggttcggca gttttgcctt cagacggcat tgctgctgcg gatgccgtct gaacgggtgt
     1441 ttgggcggtt tccgcttcag ggcgcggttg gggctttttt gcggcggttt ccttttcggc
     1501 ggtttgtgcc gatggggatt ttagttcggt attttcaatc acggcatttg catcacacga
     1561 tgctgccgcc aagggcgcaa acgccagcat acgcagcagg gtcatcataa agccggcgta
     1621 ttcgtcgggg gcgaggctga ggtcgcgttt gccgtggacg gcgatttggt agtaaagctg
     1681 gatttgttcg ccgcttatgg tttgggcgag gcggtgcaaa atatcggaat cggggtcgtc
     1741 gtgcgccaag gcattcggca ctgcctgtat cagggcgagg tgttgcagca gtatggcaag
     1801 ttcgcccaag gcgttgtcaa agccgacggc acacgccgcc atttcctgcg ctttggcggt
     1861 cagggctgcg ccgtcttggt tgatgatgcc tgtcagcagt tcgtaaaggt attgtttgtc
     1921 aaccgcgccg atcatttggc ggacatcgtt ttcggcaact ttgcccgaac ctagggcgat
     1981 ggcttggtcg agcaggctca aggcatcgcg catcgatccg gcggcggcac gtcccaaaag
     2041 ttgcagggcg gcgggttcgt aggcgatttt ttcgctgtcg aggacgtggg cgaggtggtc
     2101 ggcaacctgt tgcgcggtca tattgcgtaa gacgaattgc aggcagcggc tcaagacggt
     2161 aacgggaact ttgtgcggat cggtggtggc gaggatgaat ttgacgtgtt cgggcggctc
     2221 ttccagcgtt ttgagcatag cgttgaacgc gcttttggaa agcatatgca cttcgtcgat
     2281 gatatagact ttgtattttc cggcggtcgg tgcatattgg gcgttttcca agacttcgcg
     2341 gatgttgtcg atgcctgtgt tggaggcggc gtcgatttcc agcaggtcga cgtagcgtcc
     2401 ggcatcgatc tgcgtacagc tttcacatac gccgcaaggt tcgccgtgtt gcgcgttttc
     2461 gcagttgagg cttttggcaa ggatgcgggc gatggtggtt ttacctacgc cgcgcgtgcc
     2521 ggtcagcagg taggcgtggt gcagcctgcc ttcgtccagg gcgttttgca gggctttgac
     2581 gacgtgttcc tgaccgacta agtcggcaaa ggttttgggc cgccattttc gggcgagaac
     2641 ttgataggcc atgtttttct cttggtttcg gtcgtgatgt ttctgtcggt gcgtcggaat
     2701 gccgtctgaa cggcggtctc gggcggcgta ttctagcact ttcggcttac tgtccgcgca
     2761 agaacagtgc gtccaattct ttcaaagtca gtttgaccca agtcggcctg ccgtggttgc
     2821 actggttgct gcgcggcgta ttttccatat cgcgcagaag ggcgttcatt tcgggcaggg
     2881 tgagccggcg gccggcgcgg atcgagccgt ggcaggacat ggtggcgagg atgcggtttt
     2941 cgtgttcctc gatggtttgg ctgctgccga cttgggcgag ttcgtttaat acgtctttgg
     3001 cgagcgagac gacatcggct ttgccgagca tggcgggaac tgcacggacg gcgagggtgt
     3061 tgccgcccat atcggataat tccagcccga agcctgccag cgtttcggca taatcggcaa
     3121 gggcggcgca ttcttcgtgg gacgcggcaa aggttacggg aataagcagg cgttggcttt
     3181 gcaggttgcc gttttcctga cgttggcgtt tcattttttc gtagttgacg cgttcggcgg
     3241 cggcgtgcat atcgatgagc aacaggctgt cttcggcttg ggcaagaatg tagatgccaa
     3301 gtaattgggc aatggcaaaa ccgagcggcg gcagttcgga ttgggacggg atgccgtctg
     3361 aaagcggcgt atctgtttgg ggagcaggcg tttcagacgg catattgccg aaacgtgcct
     3421 gctcgaatcg gcttaactca aggtcgatgt cgtcggtttt tttgtaaagt tcggcgtaag
     3481 tattcattgc cgcgcggctt tcgcgcaggg acaggctgcg ttgttgcggc gcatatgcgg
     3541 actgataggg catgggcgcg gttttgcctg atgaaccaaa ggcattgtgt gtatctgatt
     3601 tgttgcctgt cgggtagttg gatacgctat caaacagatt ttcgctgtcg ttttcagacg
     3661 gcattggggt ggagacaacg ccggtaatgt catgcaacac ttcgcctgcg ttgccgacgc
     3721 tttcggtcag gttggcgcgt gtgtcggcaa gggctttgtt gagcgtgtgg aacacaagtt
     3781 ggtgcacctg ctgactgtcg cggaagcgga tttcggtttt ggtcgggtgg acgttgacat
     3841 ccacggcttc gggcggcagg tcgagaaaga ggacgaaggc gggagtgagt gcgttgtgca
     3901 atacgtcgcg gtatgcctgc ttgacggcgt ggagcatcac tttgtcgcgc acgaagcgat
     3961 ggttgacgaa gcagtattgt ttgtcggttt tacctttggc gaaagtcggt ttggcaatcg
     4021 caccatagag ccgcagcgcg ccgttgccgc tgtcgattcc caatgatgcc gtctgaaagt
     4081 cttcgccgac aatggcggca atccgttcat gcaggctttg tgcagggagt ttgaacactt
     4141 gtttgccgtc gcgtttgagc gagaaggcaa tgtgcggatg cgccagcgcg aggcgttcga
     4201 gcatggtggc gcagtgggcg tattcggtgt tttcggattt gaggaacttg cgccgtgcgg
     4261 gggtgttgaa gaagagttcg gcggcttcga tggtggtgcc gacggggtgg gcggcggcgg
     4321 tggggctgct gagtttgccg tcttcggctt tgacttgggt cgcgtgcgaa ctgtcgttct
     4381 gacggctggt cagggtcagg cggctgacgg aggcgatgct tgccaaacct tcgccgcgaa
     4441 agcccatact ggcgacgtgt tccaaatcgt ttaaggtttt gattttgctg gtggcgtggc
     4501 ggtggagcgc aagttcgatg tcgtcggggt ggatgccgcc gccgttgtcg ctgacgcgaa
     4561 tcaggcggat gccgccgccc gccagctcga cttcaatcgc cgttgcgcct gcatcgatac
     4621 tgttttcaac gatttctttc aaggcgttgg cagggcgttc gaccacttcg ccggcggcga
     4681 tttggttgac aagatggtcg ggcagggcgg cgattcggga cataaggcgg gcttccgttg
     4741 caaaaaacgt cttattctat aataaaaccc cttatctttc tgcccgtatt cttgataaaa
     4801 gccgtttatc ccgtttggaa aataccagta taatcacgcc atattccgta aaattggagc
     4861 acaaagatgt atcactacca atccgatgcc acacaattcc tcaaccgcct gattgaagaa
     4921 aaacctgagt tggaacagca gcgtttggaa aacagggggc ttttgtggga tgtcgaactc
     4981 aatcccgaag aacaggaaaa cttcgaggcg gcaaaagtgg cgaaaaaacc ttatacctat
     5041 taccaagact gaatccgccg aaatgacgac ccatctgtcc aatgtcgcac cggacctgca
     5101 aaactatttg aacgccatcg gcgaacccga acatcccgtt ttgacgcggc tgcgcgagaa
     5161 gaccgggcat caccgtatgg gcaaaatggc gattgcgcgc gaacaggcgg cagttttggt
     5221 ttggctggca aagctgatcc gtgcggaaaa atatctggaa atcggcgtat ttaccggata
     5281 cagcagcacc gcgcttgcat tggcactgcc cgaacacggg cggattaccg cctgcgacat
     5341 caatgtaacc tttaccgata cggcgcgtca ggtttggaac gaggccggtg tggcacataa
     5401 aatcagcctg cacctgcaac ccgcattgct gacattggat gatttgattg cacagggtga
     5461 agccggaagc tacgatttgg cactgataga cgcagacaaa ccgcccacgc cgcaatattt
     5521 cgagcgttgc ctcaaactcg tccgtcaagg cggcatcatc gccatcgaca atattttgct
     5581 gaacggaagg gtgatgcgcg aagcggcttc cgatgcgccg cccagcgtcg gcatcctcaa
     5641 agatttcaat caaaacctgc cgaacgaccc gcgcatcgtc cccatcaccc tgcccgtcgg
     5701 cgacggcttg accctgcttc tgaaaaaata atgaagatca aattaccgct ttttatcatt
     5761 tggctgtctg tgtccgcctc ctgtgcttcc gtttcacccg ttccggcagg cagccaaacc
     5821 gaaatgtcga cacgggaaaa tgcttcagac ggcattccct atcccgttcc gaccttgcaa
     5881 gaccgtttgg actatctgga aggcaaaatc gtccggctgt cgaacgaagt ggaaacctta
     5941 aacggcaaag tcaaagcact ggaacacgca aaaacacatt cttccggcag ggcatacgtc
     6001 caaaaactcg acgaccgcaa gttgaaagag cattacctca ataccgaagg cggcagcgca
     6061 tccgcacata ctgtcgaaac cgcacaaaac ctctacaatc aggcactcaa acactataaa
     6121 agcggcaagt tttctgccgc tgcctccctg ttgaaaggcg cggacggagg cgacggcggc
     6181 agcatcgcgc aacgcagtat gtacctgttg ctgcaaagca gggcgcgtat gggcaactgc
     6241 gaatccgtca tcgaaatcgg agggcgttac gccaaccgtt tcaaagacag cccaaccgcg
     6301 cctgaagcca tgttcaaaat cggcgaatgc caatacaggc ttcagcaaaa agacattgca
     6361 agggcgactt ggcgcagcct gatacagacc tatcccggca gcccggcggc aaaacgcgcc
     6421 gccgcagccg tgcgcaaacg atagtttttc catttttccg ctttttaccc catccgcacc
     6481 aaggaggatt tatgatacaa atcggcatca tcatgggcag caacagcgat tggcccgtta
     6541 tgcggcaggc agcgcagttt cttgaagagt tcggcgtaga atatgaggcg cgcgttgttt
     6601 ccgcacaccg caccccggat ttgatgttcc aatacgccga aaccgcacgg gcgcgcggca
     6661 tcaaagccat catcgcgggt gcgggcggcg cggcgcattt acccggtatg gttgccgcca
     6721 agaccaccgt ccccgttttg ggcgtacccg tccccagcaa atacctgcgc ggcgaagatt
     6781 cgcttttatc gattgtacaa atgcccaaag gcgtacccgt cgccacattc gccatcggcg
     6841 aggcaggcgc ggcaaatgcc gcattgttcg ccgtttccat gctcgccaac gaaacccccg
     6901 aactggcgca aaaactggca gacttccgag ccaaacagga acaggctgtt ttgaatatgg
     6961 aattggaaca aatttaaaac ccaccgtctg cgaatatata gtggattaaa tttaaaccag
     7021 tacggcgtta cctcgccttg ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc
     7081 tgatttttgt taatccacta tacaaatgcc gtctgaagcc ctgtttcggg tttcagacgg
     7141 catttttgcg aacaactttt tatgcctgtt ctttttggcg cattttttct gccattagtt
     7201 cgtgcagggt tttcttcgcc ggtttcatcg gcacgcggtt ttgcgtccaa cccaactgtt
     7261 tgcgcggggt caggttgcgg aacttggtgg ctgcccaacc gaaggcgcgg taggttttgc
     7321 tgccgctgaa aataccgttg aatgtgcgcc acgccatttg ttcgccgaag gtatgcgatg
     7381 cgccttgccc ccggatgggg tgcggcacgg tttcggtcgg cgaacgttgc gcttcaacgc
     7441 gcaaacgctg catttgttcg gtaatcggga tgcgtaccgg acaaacttcc acgcacgcgc
     7501 cgcacatcgt gcaggcggtc ggcaggtcgc gagtggcatc caagcctaac aggtgcgggg
     7561 aaataatctc gccaatcgga ccgggatagg ttgtgccgta tgccgcgccg ccgatgcggg
     7621 tataaaccgg gcaatggttc atacacgcgc cgcaacggat acattgcagg gtgcggcgca
     7681 tttggtcttc ggcataagcc tggctgcggc cgttgtcgag cagaaccaag tgcatttctt
     7741 gcggaccgtc taattcttca ctgcggcgcg ggccggtaat catattgaaa taagtggtaa
     7801 tgttctgacc aatggcagaa cgcggcagca ggctgtacaa gggtgggatg tcggacaatt
     7861 tcgccaccac tttttcaatg ccggtaatag cgatatgcac gggcggtacg gtggtactca
     7921 agcgaccgtt gccttcgttt tccaccagac acagcgtacc tgtttcagca acggcaaagt
     7981 ttacgccact caaaccgaca tcggcagtgc tgtaaatatc gcgcagtgct ttacgggcga
     8041 agccggtcag ttggtctaca tcgtctgtca gcggcgtacc gaggttttgg tggaacagtt
     8101 cgctaacctg ttctttggtt ttgtggatag caggcatcac gatatgggtc ggtttttcgc
     8161 ctgccatttg gacgatgaac tcgcccaagt cgctttctac cgctttaatg ccttttgctt
     8221 caagataatg gttcagctcg atttcctcgc tgaccatcga tttgcctttg accatcagct
     8281 tgccgttttt ggctgtgatg atgtcgtgga taatttggca ggcttcggtc ggggtttctg
     8341 cccagtgcac tttcacgccc aacttagtca ggttttcttc cagctgctcc agcagggctg
     8401 gcaatttaga caatgagcgc tgacggacgt gttcgcacaa atcgcgcagg ctttgcagct
     8461 cttcttcgtc ggtcaaaacg gctttgcgtt tggtcatcag catatccatc gcggtacgca
     8521 ggcttttgcg caaaggcttg tcttgaaggg aaattgcggc gttttgcttg aaagtttccg
     8581 gcttcatgtg aaatttgatg gtttgcgtag tcatgcgttt tcctccaaat cggcagggga
     8641 aatgtggtcg ggcaggatgg cgaggatgac caaatcgcgc gggccgtgcg cgccgtaagc
     8701 aagcgtcagt tggatgtctg cggttttgga cgggccggaa atcaggaata cattggtcgg
     8761 cataccgttt tccaccagtt tttcgccttc gacggcatta tgaaactcgt tgtacatctt
     8821 ggacgtatcg aacaggcaga aatgcacggg cggaacgagg cttaaagtac gcggttcttc
     8881 ggggctggaa aacagcatca gcgtgccggt gcgggcgatg ccgcattgcg cgccgctgaa
     8941 gcccgcatcg atgttcgtga aaaactcggt tttccaagta tcgatttcgc gctcgaaggc
     9001 aatcggttcg atattgctgt ccgccaatgc ggcacgggca atttgtccgt gttcggtcgc
     9061 caagggcagc aggatgtttt tcaaaccctt gccttctgcc gcttcgcgga aaacctgcat
     9121 ccaattgctt ttcgtcaccc aataaatttc ggttttgacc gcacgcatag cggcagccca
     9181 atgtttcaga cgctcaactt cgctgcccca agaaacaccc atttcacggt aataatcaaa
     9241 aaccgcaggt tcttccatcg gcaatgcgtc ggcttttttc agttttgcca aaatattttc
     9301 acgcgcgctc atgctttgcc tccggtgcgt tccaacaaga aggatgcgat atgtttcgga
     9361 cgcggcatat ccggctcgtc cttggcgatt ttgccgccga tgttcatcat acagccgcag
     9421 tccgcgctga tgatttcggt tgcgccggtt tctttcagcg cggcgacttt gtctgttacc
     9481 attgcgccgg aaatatcggc ttgtttgacg gagaatgtgc cgccgaagcc gcaacattcg
     9541 ctttcgtggt cgtggacgat gcgttcgacg ttttccatac cgtcaatcag ttgccagcct
     9601 gaaagatgga cattcatttc gcggcgggcg gcgcaggaag tgtgaacggc gactttgagc
     9661 ggttcgccct tgtcttcggg tttgaaaccg atggcaagca ggaaatgggt aaactcgatg
     9721 atgcggccgg cgcaatccac agccctttcc tcgtactcgc tgcctttaaa cagcgtcggc
     9781 cagtggtgtt tcatcatgcc gccgcacgag ccggacggca cgacgatcgg ccagttttca
     9841 gggaaaaggt cgagttgtgc tttggcgaca tcgaaggctt cggtcggatg gccggatgaa
     9901 taggcaggct ggccgcagca gctttgcgcc atcgggaaat ggacgcgtat gccctgctgc
     9961 tcgattaggg taatggcatc catgcctgct tcgggcataa aaagatcaag gacgcaagtg
    10021 ccgaaaaaat agacatcggt cggattgctg tcgtatcgga tgatttttgg gggaatggtt
    10081 gcgctcatgt tttttgataa cgggaaaccc gtttttctct gtagaaaggt aagcgtttac
    10141 tttaagtaat tgactgttgc gggtcaagtc taattttaaa aaataatccg gtttttctta
    10201 caaactgccc ccataacgct tactgtacct taatctgatg gttttcgata ataattatca
    10261 ttacaatgca atgccggttc gtttgcttgt gaacattcaa gatgccgact ctgacggcat
    10321 tcagacagca tctgaaaaca ataacggcac aaggatgggg tacaatcgcc ctatggaaaa
    10381 aaacacccta tccgcccgcg caccatcccc ttggctgccg cttctgctgg caattgccat
    10441 ttttatgcag atgttggatg cgaccatttt aaataccgca ctgcctgaaa ttgccgccga
    10501 cctgaatgag tcgcctctgg atatgcaact ggcagttatt tcctacacgc tgacggttgc
    10561 cctgctgatt cctttgagcg gttatttggc ggacaggttc ggaacgaaaa aagtcttttt
    10621 cggttcgatt gccgttttta tgctcggatc ggcattgtgc gccgcatcgg gttcgctgtt
    10681 tgaattgacg ctttcccgtg tcgttcaggg catcggcggt tcgatgctgg ttccgatacc
    10741 gcgtctgacc atcttgcgtg tgtacgacaa gtccaagctg ctcaatgcca tcaattatgc
    10801 ggttatgccc gcattaatcg ggccggtttt agggcctttg gcgggcggtt atttggtcga
    10861 atacgcttcg tggcactgga ttttcctgct caacctgccc atcggtctgc tgggtttcat
    10921 attgggacgc aacatcatgc ccgatattaa aggcagtaat atctctttag acttcaaagg
    10981 ttatctgatt ttttctgccg ccgcgtgcct cttgttactt tcggcagaaa gcctgtcgca
    11041 cgcgctgcct ccgtattttg cactgttgcc gctgtgcggc ggactgctgt ttgcacgccg
    11101 ttatttccga catatgaaaa ccgcgtccaa accgatttat tccgccgacc tgtttctgat
    11161 acgcactttc cgtctgggac tggcgggcaa tctgttcagc cgtctcggca tcagctcgat
    11221 tccttttctg atgcccctga tgtttcaaat cgctttcggc ttcggcgcaa gcctgtcggg
    11281 ttggctggtc gcacccgtcg ccctgtcttc gctgctggtc aaaccgctga ttgcaccgct
    11341 catgaaacgt ttcggctacc gcacggtact gctttggaac accaagctgc ttgccgcctt
    11401 catcatgctg ctcgccctgc ctgacggaaa ctcgccgctg tggatttggg ttttcctctc
    11461 gctggcgatc ggcgcgtgca actccctaca gttttctgcc atgaacacac tgaccctcgc
    11521 cgatttgcgc ccgcaacaaa caggcagcgg caacagcctg atggcggtca accaacagct
    11581 tgccatcagc atgggcattg ttgccggcgc attaatcctt aaaaactgga catttctgat
    11641 accggcttct tcaggtctgc attccgcctt ccgtatgacc ctgctcagca tcggcggcat
    11701 cacccttgca tcatcgctgg ttttcaaacg gctgcacgtt tcagacggca ccaacctgac
    11761 acggaacaca ccgtcctgaa gcggtccaca cgcaaaactt ttacccgttt caacgtttgg
    11821 attatgatac cgcacttcca tgcgcgccaa ccccaaaaca caggcaatgc cgtctgaaac
    11881 catatccctg atgaaaacac gcagcctaat ttccctttta tgcctccttc tctgttcatg
    11941 ttcttcatgg ttgcccccac tggaagaacg gacggaaagc cgtcatttca atacttccaa
    12001 acccgtccgc ctggacaaca tcctgcaaat ccggcacacc cctcatacca acgggctatc
    12061 cgatatctat ctgttgaacg acccccacga agcctttgcc gcccgcgccg cccttatcga
    12121 atctgccgaa cacagcctcg atttgcaata ctacatctgg cgcaacgaca tttccggcag
    12181 gctgctgttc aacctcgtgt accttgccgc agaacgcggt gtgcgcgtac gcctgctgtt
    12241 ggacgacaac aacacgcgcg gattggacga cctcctgctt gccctcgaca gccatcccaa
    12301 tatcgaagtg cgcctgttca accccttcgt cttacgaaaa tggcgcgcac tcggctacct
    12361 gaccgacttc ccccgcctca accgccgcat gcacaacaaa tcctttaccg ccgacaaccg
    12421 cgccaccata ctcggcggac gcaatatcgg cgacgaatac ttcaaagtcg gtgaggacac
    12481 cgttttcgcc gatttggaca tcctcgccac cggcagcgtc gtcggcgaag tatcgcacga
    12541 cttcgaccgc tactgggcaa gccattccgc ccacaacgcc acgcgcatca tccgcagcgg
    12601 cgacatcggc aagggtcttc aagcactcgg atacaacgac gaaacgtcca gacacgcgct
    12661 cctgcgctac cgcgaaaccg tcgaacagtc gcccctctac caaaaaatac agacaggatg
    12721 catcgactgg cagagcgtcc gaacccgcct catcagcgac gaccctgcaa aaggactcga
    12781 ccgcgaccgc cgcaaaccgc cgattgccgg gcggctgcaa gacgcgctca aacagcccga
    12841 aaaaagcgtc tatctggttt caccctattt cgttcccaca aaatccggca cagacgcact
    12901 ggcaaaactg gtgcaggacg gcatagacgt taccgttctg accaactcgc tgcaggcgac
    12961 cgacgttgcc gccgtccatt ccggctatgt caaataccga aaaccgctgc tcaaagccgg
    13021 catcaaactc tacgagctgc aacccaacca tgccgtcccc gccacaaaag acaaaggcct
    13081 gaccggcagc tccgtaacca gcctgcacgc caaaaccttc attgtggacg gcaaacgcat
    13141 cttcatcggt tcgttcaacc tcgacccccg ttccgcgcgt ctcaacaccg aaatgggcgt
    13201 tgttatcgaa agccccaaaa tcgcagaaca gatggagcgc acccttgccg ataccacacc
    13261 cgcctacgcc taccgcgtta ccctcgacag gcacaaccgc ctgcaatggc acgatcccgc
    13321 cacccgaaaa acctacccga acgaacccga agccaaactt tggaaacgca tcgccgcaaa
    13381 aatcctatcc ctgctgccca tagaaggttt attatagaaa tatagcggat
//
LOCUS       DAWIVB010000036        13386 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWIVB010000036 DAWIVB010000000
VERSION     DAWIVB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.036"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(110..538)
                     /locus_tag="WIX60_000770"
     CDS             complement(110..538)
                     /locus_tag="WIX60_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ5305065.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WIX60_000771"
     CDS             complement(603..2351)
                     /locus_tag="WIX60_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ5305066.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WIX60_000772"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WIX60_000772"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ5305067.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WIX60_000773"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WIX60_000773"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ5305068.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WIX60_000774"
     CDS             4754..4924
                     /locus_tag="WIX60_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ5305069.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WIX60_000775"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WIX60_000775"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ5305070.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAVSEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6085..6279
                     /locus_tag="WIX60_000776"
     CDS             6085..6279
                     /locus_tag="WIX60_000776"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ5305071.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6481..7890
                     /locus_tag="WIX60_000777"
     CDS             6481..7890
                     /locus_tag="WIX60_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ5305072.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTSLLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7992..11201
                     /gene="recC"
                     /locus_tag="WIX60_000778"
     CDS             7992..11201
                     /gene="recC"
                     /locus_tag="WIX60_000778"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ5305073.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSEMAGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPKGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLTDIVRIWRQPATVGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAEMRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11269..12837)
                     /gene="mtrF"
                     /locus_tag="WIX60_000779"
     CDS             complement(11269..12837)
                     /gene="mtrF"
                     /locus_tag="WIX60_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ5305074.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIYIVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13169..13381
                     /locus_tag="WIX60_000780"
     CDS             13169..13381
                     /locus_tag="WIX60_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305075.1"
                     /translation="MKNEIQKNMDKYNPWHEDDFESYEDIARDVSLTTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3347 a         3229 c         3554 g         3256 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gatattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg ttccggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 ataccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggcg gcatgccagc ggtttttggc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggtgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 tacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcgccgg gcgtgaagcc ttacaatgcc ctgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactcgcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccgtt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtgggcggtc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattcccg
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cacctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgtgctctc ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgtgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccgac aatcagagca accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaaa gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcagtaccat gccgcgcaat cagggttggt attacacgat ggatgaaatc
     6901 cacagcgata tgatgctcgg tgcggcaggc gattatcttt tggaaacggc agcttcactg
     6961 accattatta tggttgtcag cggcttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgtcaaa aggcagggcg cgttcttggt ggcggaatct gcacggcacg
     7081 tttggaactt gggtgtcgtt gattttgctg ttgttctgcc tgtcgggtat tgcttgggcg
     7141 ggtatttggg gcggcaagtt cgtacaggct tggagtcagt tccctgccgg taaatggggt
     7201 gtcgaaccga accccgtttc agtcgtgccg acccacggcg aggtattgaa tgacggcaag
     7261 gttaaggaag tgccgtgggt tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc tgacgagccg atgacattgg aaaccgtcga ccgctttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaggattc tatgagttac gacatgatca gcccgtttgc cgaccgcacg
     7501 gtacatatcg accagtacag cggcaaaatc cttgccgaca tccgttttga cgattacaac
     7561 ccgttcggca aatttatggc ggcaagcatt gcgctgcata tggggactct gggctggtgg
     7621 agcgtgttgg cgaacgtctt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgaccgga gcggtgggca tcgttccgcc ggcgcagaaa
     7741 gtcaagctgc cggtttggtg gatgatggca ttgccgctat tggcaatcgc actgctcttc
     7801 ccgacctcac tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga ccaaatgccg tctgaaaggt tcagacggca
     7921 ttttgtttga aggcggacgg gggaaaggct atataatccc gaatacttga ccacagcttc
     7981 tgtttgaaat catgttttat ctgtatcaat ccaaccgtct tgaaacgctg gcggcattgt
     8041 ttgcccgcat tcaaaaagtc aaaccgctga aatcggcttt acagcccgaa cagattattg
     8101 tgcagagtca ggggatgcgc cgctacctca atacctgcct cgcccgcgat ttgggcgtgg
     8161 cggcgaattt gtcgttcagc ctgcccgccg gcctgacgtg gaagctgatg aaaaaactga
     8221 ttcccggtat tccggaactc agcccgtttg cgcccgaagt catgcgctgg cggctgctgg
     8281 atttgttccg cagcgaggca tttcggaata cggcagaatt tgaagatgtg aggaatgtgc
     8341 tgcaagacta tctgggcagc ggcgaatcgg cagattacca gcttgcggga cagcttgcgg
     8401 acatattcga ccaatacctc gtgtaccgtc ctcagtggat agacgcttgg cagcagggca
     8461 ggcggctcgg tttgggcgac gacgaaatct ggcagtccaa actgtggcgt tacctcgacg
     8521 acggcaggca gagcgcgccg caccgtgtcg cgttgtggga aaagctgttg gaatctttga
     8581 gcagtgataa gctgcccgag cgttatttcg tgttcggcat ttccacgatg gcgccgatgt
     8641 atttgcaact tttgcacaag ctgtccgaac attgcgacgt gttcgtgttc gcactcaatc
     8701 cgagcgggat gtactggggc aacgtcatcg aagcggcgca aatcctcaaa ggtggcggcg
     8761 atcccgattt aactcaggca gggcatccgc tgctcgcctc attgggcaag caggggcggg
     8821 actttttcga ctttttgaac gaaatggaaa tagaaggcga aacgccggta tttgaggaag
     8881 gcgggcgcga tacgctttta cacgccctgc aaaccgatat tcaaaacctg aaaatgccgt
     8941 ctgaaatggc gggaagcgtc aacacgggcg acggctcgat acgcatcgtc tccgcacaca
     9001 gccctttgcg cgaattgcag atactcaaag acaagctgtt gaaaattctg catgaacatc
     9061 ccgattggca gccgcacgat atcgccgtat taaccccgaa tatcgaatcc tatacgcctt
     9121 ttatcgaagc cgtgttcgga caggcgcagc ccggtgcgca ggcattgccg tattccgtgt
     9181 cagacgtgaa aatcagccgc cgccaaccgc tgtttcatgc tttgtcatgc ctgttcgact
     9241 tgttggaaag ccgatttgaa gtcgataaag tgcttgtgct tttagaaacc gcgcccgtgt
     9301 tgcgccgttt cggactgact gaggacgatt taccgctttt gcacgacatg gttgccgatt
     9361 tgaacgtcca ctggggtttg gacggagaaa tgcgcggcgg cacggatcag ctgttcacct
     9421 ggaagcaggc ggtagaacgc atgatattgg gctggatgct gcccaaaggc ggcaatccga
     9481 tgtggcagga tgtcagcgcg tggtatgccg acgtgaatca aaccgccatg ttcggacgtt
     9541 ttgccgcctt cctcgaaacc ctgacggata ttgtacggat atggcggcag cccgcaacgg
     9601 tcggcgaatg ggtggcgcgt tgccgggatt tgcttgaaac attgttccaa gctgaagccg
     9661 atgaccaaaa gtcagtccaa aaccttgaaa acgaatgggt caaatggcag gcggaaaccg
     9721 aattggcgca attttccgga cagttgccgc cgcacaccgt catccgccat atccgacgct
     9781 ttctcgacag cgaaagcgag gcaggctttt tacgcggcgg catcaccttt tgcagtatgg
     9841 tgccgatgcg gagcctgccg ttcaaagtca tctgcctgtt gggtttgaac gacggagatt
     9901 ttccccgtaa taccaaagcc gccgtattcg acctgattgc caaacatccc gccaaaggag
     9961 accgcgcccg ccgcgatgac gaccgctacc tgttcctcga agccctcatc agcgcgcgtg
    10021 aaatcctcta cctgtcctac atcgggcgcg acatccgcaa agacgaagag cttgccccgt
    10081 cttccctgtt gggcgaactc atcgataccg ttgccgctat ggcgggcatc ggtagccgcc
    10141 aacttgcaca aaacctgata gaacagcatc cgctgcaagc cttctcgcgc cgatattttc
    10201 aagaaggcgg acgttcagac ggcatattcg gcacgcgtac cgactacgcc gccgcgctcg
    10261 gacaaacgcc cgaaccgccg caaccctttt tcgatcaacc cgtagaaaac gccgaacctg
    10321 ttgccgaaat cggacaggac gaatttatcc gtttctggcg caaccctgtc aaagtatggc
    10381 ttcagcagca gcttgcgtgg agcgaacccc atatcggcga agcctgggag cctgccgaac
    10441 ccttcgagcc gcaacacgcc gatcaaatcg ccgaaatcta catcgaagca cggcgcgaag
    10501 gacgggattt tgcccaaacc gccgcccgca tcggggcgga aagcctcctg ccgtcgggag
    10561 agttgggcag actttggcag caggacttcc aaactgctgc caaacaaatc gacacggcgg
    10621 ttttaaacag ccccaaactg ccaccgcttt catatgccat accgtcggac gggcaaatcc
    10681 tgaaaggcag cttgggcaat ctgtaccgct gcggacaagt gttttacgcc tacggcaaac
    10741 ccaacgcgcc gcaacgtatc gcttttctgc tggaacacct gatattttgc gccgttatgc
    10801 cgtctgaagc cgaaatgcgg caaaccttta tcgtccaatc cggagaaacc gaaatattgg
    10861 cggaaatcgc gcaagacagg gcattgcagc tattgtcgga atggatggcg tttttcaata
    10921 tcgggcaaaa ccgcccgctg ccgttttttg ccaaaacctc gcttgccacc gccgaagcgt
    10981 ttgcccaaaa acaagattgg gaagccgccc tgaaaaaagc ccaaaccgcc taccacggca
    11041 gcaaagtcag caaagggcag aaagactata ccgaagtcgc cctcgtgttc ggcaacgcaa
    11101 gccaaaaccc gctcgaacag cccctgtttg aaaacctcgt ccgtctgctt gccgacacgc
    11161 ttgccgcagc ggaaaaaaag gaagaggccg gagaagcctg acgggataga aaggcaaatg
    11221 ccgtctgaaa gcgtttaagc atttcagacg gcattttgtt tatcgtgttt aaggtgcggg
    11281 atagaatgtg ggcgcgccgg gaccgacggg caggcccaaa acaaataccc aaatgcagaa
    11341 taaggcaatc cacgcaatca agaagaaagc ggaatacggc aacatcatag aaatcagcgt
    11401 acccacgccc gcatcttttt tgtatttgat caccgtcgcc ataatcagcc cgaaataact
    11461 catcatcggc gtaataatat tggtaacgga atcaccgatg cggtaagcgg cttgaatgac
    11521 ttcgggcgcg tagccggcca acatcagcat agggacgaaa atcggcgcag ttaccgccca
    11581 ttgcgcggag gcggagccta tcatcagatt gataaaagca caaattaaaa taaaaccgat
    11641 aaacaacacg ctgccgccca agccgacttc ttttaagaac gtcgcccctt taacggcaat
    11701 atattgccca atattcgtcc aattaaaaaa tgcgacaaac tgtgcggcaa aaaagatgat
    11761 gaccaaataa agccccagag tactcatcga ttcggccatc gcattaacga cttcctgttc
    11821 gccgcgcaaa cttcgggtta cccggccata aacaatgccc ggcagtgcaa acaacaagaa
    11881 aataaaaaca acaatcgatt ttaaaaacgg cgaaccggaa accaatcctg tttcaggatg
    11941 acgcaaaata ccgtcggcag ggacgatgct ccaagccaat agggcggata aggcaacaaa
    12001 caccacgcca gcccaaatta atcctttata ttccaaaggc gtgatttcat tggaatgccg
    12061 aatgtctttt tcttcttgtg acaaatctga ttgataaggg cccaattgcg gttcgacgat
    12121 tttttcagta acaaaataac caatcaaagc aatcacaaac gtactggcta ccataaaaaa
    12181 ccagttggct tcagggccta cgacgtagtc gggatggatg atttgcgccg cctgttgggt
    12241 gatgcctgcc aagagcggat cgattgtgcc taagaacaga ttggccgaat aaccgcccga
    12301 aacgccggcg aaagccgcag ccagaccggc aagcggatgg cggccgaggg aatgaaagat
    12361 gatggcggac aaagggatta ggacgacata gcccaattca gaagcggtat tagataaaat
    12421 ccctgtaaaa acaaccataa aagtagtgag tttgcgtggc gattttgtga gcaataagcg
    12481 cattaatgcg gaaatcaagc ccgatttttc cgcaatcccc acgcccaata aagaaaccaa
    12541 caccgttccc aacggcgcga aaccggtgaa atttttaacg gtatgcgtca ggattttgat
    12601 caaaccgtcg gcattgagca ggctgacaat gtaaatcaaa ccgtcatcgg cacgtccttt
    12661 cgcaccaaca gggcgcggat cggggacgga tagtccgaaa tacgcaccga cggcagaggc
    12721 aatcagcaat aacacaatga aaataataaa aagcgtaacc ggatgcggca acatattgcc
    12781 cagccattcg actgtgcgta aaaatcgtcc gtcccgttgc gtatcggttt gactcatctg
    12841 cttctcctta aaaaaatata ttgagtgcat taaatttttg aatgtaacat gaagtgtttt
    12901 gtaagcaaac taaaataatc gcatatgtat ataaccgcaa tagattaaaa cagaaaatgc
    12961 ccacatcgtt attctcataa aggcggtaat ccgaaaactt gacgttaaag ccttatcagg
    13021 aatgactgga actcaaaaaa cggtttcccg ttttcgcgga aatggcggga ttttcagttt
    13081 ttatttgcaa aaatgctgga gaaaaatgca gtttacattt tgaatggtat tgttattaat
    13141 tgtaaataaa tattggaagg taaatgatat gaaaaatgaa attcaaaaaa atatggacaa
    13201 atataatccc tggcatgaag atgattttga gtcgtatgaa gacattgcca gagatgtatc
    13261 gctgacgaca gataaaacgt tcattgaaca ttatttgtta gaagtttatt cagaagaaaa
    13321 cggacatttt gaccaagaga atgtccatgc aatgatagaa gaaattaaaa atgcaattta
    13381 gtgaat
//
LOCUS       DAWIVB010000037        13108 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWIVB010000037 DAWIVB010000000
VERSION     DAWIVB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.037"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            389..1105
                     /locus_tag="WIX60_000781"
     CDS             389..1105
                     /locus_tag="WIX60_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5305076.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1208..1633
                     /locus_tag="WIX60_000782"
     CDS             1208..1633
                     /locus_tag="WIX60_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305077.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            1877..2077
                     /locus_tag="WIX60_000783"
     CDS             1877..2077
                     /locus_tag="WIX60_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305078.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2145..2504
                     /locus_tag="WIX60_000784"
     CDS             2145..2504
                     /locus_tag="WIX60_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305079.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVSNTSSTLKMVRSN"
     gene            2529..3383
                     /locus_tag="WIX60_000785"
     CDS             2529..3383
                     /locus_tag="WIX60_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ5305080.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTPLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3453..3668
                     /locus_tag="WIX60_000786"
     CDS             3453..3668
                     /locus_tag="WIX60_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305081.1"
                     /translation="MTITEEYSIDIRVTSEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLTKDNLKNAA"
     gene            3703..3963
                     /locus_tag="WIX60_000787"
     CDS             3703..3963
                     /locus_tag="WIX60_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305082.1"
                     /translation="MKYLIRTALLAVAAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            3960..4253
                     /locus_tag="WIX60_000788"
     CDS             3960..4253
                     /locus_tag="WIX60_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305083.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4256..4447
                     /locus_tag="WIX60_000789"
     CDS             4256..4447
                     /locus_tag="WIX60_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305084.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4447..4587
                     /locus_tag="WIX60_000790"
     CDS             4447..4587
                     /locus_tag="WIX60_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ5305085.1"
                     /translation="MDTLLKIIIALSFSGAEALAVWLFITAADAVLRRFQKPSHYRRV
                     KK"
     gene            4584..4793
                     /locus_tag="WIX60_000791"
     CDS             4584..4793
                     /locus_tag="WIX60_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ5305086.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(4851..5768)
                     /locus_tag="WIX60_000792"
     CDS             complement(4851..5768)
                     /locus_tag="WIX60_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ5305087.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMGRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(6637..6993)
                     /locus_tag="WIX60_000793"
     CDS             complement(6637..6993)
                     /locus_tag="WIX60_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ5305088.1"
                     /translation="MAERNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAI
                     LTVLNQREFVCAGVLGGALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQE
                     FEAVARKWKKKPLKTK"
     gene            complement(7541..7921)
                     /locus_tag="WIX60_000794"
     CDS             complement(7541..7921)
                     /locus_tag="WIX60_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ5305089.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(8034..9106)
                     /locus_tag="WIX60_000795"
                     /pseudo
     CDS             complement(8034..9106)
                     /locus_tag="WIX60_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(9297..9386)
                     /locus_tag="WIX60_000796"
     tRNA            complement(9297..9386)
                     /locus_tag="WIX60_000796"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9350..9352),aa:Ser,seq:cga)
     gene            complement(9462..9552)
                     /locus_tag="WIX60_000797"
     tRNA            complement(9462..9552)
                     /locus_tag="WIX60_000797"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9516..9518),aa:Ser,seq:tga)
     gene            complement(9635..10414)
                     /gene="yaaA"
                     /locus_tag="WIX60_000798"
     CDS             complement(9635..10414)
                     /gene="yaaA"
                     /locus_tag="WIX60_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ5305090.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(10677..11864)
                     /gene="dapC"
                     /locus_tag="WIX60_000799"
     CDS             complement(10677..11864)
                     /gene="dapC"
                     /locus_tag="WIX60_000799"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ5305091.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRMYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLALARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            complement(11942..12394)
                     /gene="dut"
                     /locus_tag="WIX60_000800"
     CDS             complement(11942..12394)
                     /gene="dut"
                     /locus_tag="WIX60_000800"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ5305092.1"
                     /translation="MNIEVEMKVLDERMADVVPVYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVVVPIVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            12594..12959
                     /locus_tag="WIX60_000801"
                     /pseudo
     CDS             12594..12959
                     /locus_tag="WIX60_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
BASE COUNT         3560 a         3366 c         3004 g         3178 t
ORIGIN      
        1 atattaaaaa ttatcgtcat caatgcggct tccccagccc gcggcgagct ttccacgaca
       61 ggcttttttt atttgggttg gtcgtctggt ttctttgagt tcaaaaacag gtcgggatac
      121 ttaagtttta ttcgtgcagg tatcccgcgc ttcgtccaat tgaaaacgca ttgagggcta
      181 ttccctgtta ttcgaccaac ttccgcgtaa ctgccgattg attgcaacag gcgtttgtct
      241 tcattgactc ttttatccat aaataaacca aatgtttaaa tctaatgcta atattaaaca
      301 ctatgtttag ataaaaatca agtcttgtgt aacaacattt tgtttaaata tgggagaata
      361 attaagccaa ccgcgaataa gattaaaaat gacaatgcac gagacaactg acagactttt
      421 tgagatagcc aaagagcagg gagttttaaa gccggctgac atagcagagc gtctgaatat
      481 cagccaacag gctttgaaaa actgggaaag tcgtggcata gcggcaaagg cgctgcctga
      541 agtagcaaaa gcattcggtg tatctgaaac atggctgaga acaggtgaag gcagccgaac
      601 cgcgcccgtc cttattgacc ccgacctacc ccacgaagtc aaagacatcc accgcccgat
      661 gacgtggagc agcaacgacc cgctgcccga cgatgattat gttttcgtcc cctacctcaa
      721 agagagctgc ttcaaaggcg gagtaggcac gtatgaaatc cccgattaca acggctaccg
      781 cctgccgttc ggcaaatcca cgcttaaacg caaaggcatc aatcccgaca acgtgttttg
      841 ctgcaccctg accggcgaca gtatggagga aaaaatcgca gaagacgcgg caattgccgt
      901 agatacgggc gaaaccgcca tacgcgacgg caaaatctac gccttcgccc aggacggtat
      961 gttccgcgtg aagtacctga tacggcagcc tggcaacagc gttctgatac gcagccacaa
     1021 cagcggtttc tatggcgacg aaaacgcccc cttggacagc ctgaccgtta tcggcagggt
     1081 attttggtgg agcgtgctgg attgaaaaat gttgctatta cgacgagata atattgatag
     1141 ggctttcaag attgtcaaaa ataggcgttt tgattcccct tggtggcctg gtgagtacga
     1201 tgccggtatg aatttccttg gcgtacaagg agagttgaag gttcacgagc tgcatcacag
     1261 aacagcaacc ctgtgctttg agtggcttgg cgaagtatct gcgccgcgca gaaaagagaa
     1321 ctacaaagac ctcaagccaa atgtattgta tgactttgat ggttccggta agcattttgc
     1381 caaccctgac gcaaggtatc tattgcccgt cggttcaagc ggcttaatct taaaacatat
     1441 ccaaatcgat gacgaagaca ctctgttgcg gctatggtgc gcacgaaata tcccaatgcc
     1501 acaccggcta tctaaaatcc cgatgcttcg gcagtattat ctaagcaagg catggcacga
     1561 aatttatgct atcaaccaac acctaaggaa aacgaagctg atagttgatg tggcatacga
     1621 ccccacagat taaacaaccc gcccgcatta tgcgggcttt tttcatgccc cgccgaacct
     1681 gaaaacaaca caaaaccgac aaagccgcct taataggcgg tttttttgcg cctgcgaaat
     1741 ctcaaaaata aattctttta ataatcaaaa tgtttataaa ccttaaacaa aaatcttaaa
     1801 cagattgttg catattatta aacgctatgt ttaaatacac ccatcgaaac aaacaacagc
     1861 cacatggagt accgacatgg aaaccatcac catccctaaa gaaaatttcg acgctttgct
     1921 aaatctcgca agaaccgccg tcgccctgaa cgaaaatttt attccaaaaa taaattacgg
     1981 agcatccacc ataccagcag ccggctttgc cgcacttaac gagtttggaa tagcccgctc
     2041 caccgtggaa tctttggaaa ttctcaaaat taaataacct cctcggtatt gtcatcccgt
     2101 gataccggca acctaactaa cctctcttta aaggaaaatc aaaaatggaa acccaagaaa
     2161 agaaatttgt tcctgcctcc aagcaaatac cgaccgtaaa tagcggctgg ttagaccgtg
     2221 aattaggcga agccatgagt gaagcggtgc gtgcctgcct tgctcacggt aaacagtcag
     2281 aaattactgt caagctcaaa atccaacccc aaaacatcca aagcggaacg gtaaaaatca
     2341 gccacgatgt agcaaccaaa ctgcccaaag aaaaacgcga aggcggcatc gtctttgcta
     2401 cacctgacgg caacatccaa gccgacgatc cggcacaagg aaaattgaaa ctcaaacaag
     2461 tttccaatac gtcaagcacg ttgaaaatgg tcagatccaa ctaactcaaa cacaaaagga
     2521 aatctaaaat ggaaacccaa gaaaacatga ttaaaaccgc cttacaagca gctcaaaaac
     2581 ctttttttga gtttgcaccg aacaatactc cgcttgtatt cacaccagac caagacggtg
     2641 gctggagata caaatcacac cccgaattga tgcaaaaccc gtaccgcaag tgcggcaaat
     2701 tcctcatgca cgataccgcc agtcttatta agtttgtaca aaaacacaaa caggacggca
     2761 cacaaatcta cattgatgcc gatttcaaat cagggcgtat tgatgtcacc gccgtcatca
     2821 atggccatac gcaacaagct ccgggctggc tcgattttta tgccgtatac accccgcaac
     2881 acactacatc ggcatccaac tggctgaaca acaatgcgca ccgcatgaat cagatggagt
     2941 tttcccattt cctgaccaac aatgcccgca acatcgtatc caaaaatccc ggtaacgaaa
     3001 attccgttta cccgaccgcc gccgaagtat tggatttcgc gctcaatctc gagtacaccg
     3061 aaaaaaccac cttcaaacaa ggctatcgtg aacaggacgg cagaatcaac tttaccttcc
     3121 aatccgaaga ctcagggcaa acagagaaaa acctcaaaat gtttgaacgt ttcggcatcg
     3181 agttcacccc gcatcaaggc ggtgcatcct actttgttga agccctgttg aaattccgca
     3241 tcgacaaaaa cagcggcgca ctcgtcctgt ggtacgaatt gcagcaaatc gatgctgtaa
     3301 tcgaacaagc ggcaaaagac atttccgaag ccgtacaaaa agccttccct gaaatcgaca
     3361 tctatttcgg cgtaatcgcc taaaaaaaag tcgtctgaaa tcgtgaacct tcagacgacc
     3421 tccacaaaaa cccaaatccg aaagacccca aaatgaccat cacagaagaa tactccatcg
     3481 acatccgagt aacctcagaa caggggaaaa acgactacgg ctaccccacc gaacgctacg
     3541 gatgcgacat catcaacaca gacggggagc tacttgtcgg catagagcca gagtacaaaa
     3601 ccccctttgc cgccgtcaga aaggcgctca tctgtctgac aaaagacaac ctcaaaaacg
     3661 ctgcctgata ttaagccgtt accccaataa tgaggaatca aaatgaaata cttgatccgc
     3721 accgccttac tcgcagtcgc agccgccggc atctacgcct gccaaccgca atccgaagcc
     3781 gcagtgcaag tcaaggctga aaacagcctg accgctatgc gcttagccgt cgccgacaaa
     3841 caggcagaga ttgacgggtt gaacgcccaa atcgacgccg aaatcagaca acgcgaagcc
     3901 gaagaattga aagactaccg atggatacac ggcgacgcgg aagtgccgga gctggaaaaa
     3961 tgaactttca ggaagaaaac gagaacatca agaatgacat agagattaat ctcttgcatt
     4021 ccgctgaagc cgacagcgtg gaggcaatga tggacttggc ggtttacggg ctggcggcga
     4081 tggttgccaa acacaacaaa acgcgcggcg agcagcttgt gttacaggcg attgccggtc
     4141 atatgcggaa gcttttggcc gcgcttcccg aaaccgagtc attggtgcag accgggaagg
     4201 tttaccgccg acttgaagat ttgattttta aaacgtattt gccggagcag taaagatgaa
     4261 cacgaatcaa gatacgattc catttggcgg caaccttgtg atttgctgtt cgacgggaaa
     4321 cggcggggac gggcggtttt cttgtctgat taccgatcag ataccggatt taaatcttgt
     4381 cagaagcggg caggcgttcc cgatgttcat ttatgagtac gaaactagag aaaaaaggaa
     4441 agcctgatgg acaccctatt aaaaatcatc atcgcgctgt cattttccgg cgctgaggca
     4501 ttggcggtat ggcttttcat cacggccgcc gatgccgtat tacgccgctt ccagaaaccg
     4561 agtcattaca ggagagtaaa aaaatgaaca tcaacgaact gggcgcaagg atagaccgcc
     4621 cgaccatccg cgaactgatt gcctacgcaa cctgccgcaa ccgccccatt tcaaactcga
     4681 cactgctgcg tatggaaaaa gacggacgga taccgtgccg tctgaaaacc ccactcacat
     4741 cccccgtatg ggacacccgc gaagttttgg aagccctggg gttacagcaa taaaaaaacg
     4801 cccgattagg gcgttttcac attcagacgg cttttgtatt ccctactgca tcaaaccgac
     4861 gacaggttgc ggatttcggg cagcatcggg cggatttttg ccgcgtgttc cgcgtcggcg
     4921 tgtgcgttta aggcttcgag ggcgtttgcg gcggctttga ggcggctgcg tgtttccgcc
     4981 cagaccgtcc acatcgttac cgcctgtttg cagccgagct gcttcagcgg cagggaaacg
     5041 tctctgccca tttggatggc ccacgcgccg tatctgacgg caacggcgag gtcgtacagg
     5101 gcgttgccgc tgatgggcag ggcaggttgc ggtgcgggca atggttcgcg gtcgaggact
     5161 tcgccgtaca gcacgccgtt ttcaactgcc acgcggccca tcacgcttaa aacttcgggg
     5221 aactgttcgg caggtacttc tttgtagctg catccgaact tgcttttgac ggcagaccaa
     5281 agggtaatgg cgatacgcgc ctgcgcttct ttgggtgcgg atttggtcag ggcgttgtgc
     5341 agttttttga cggcttcgat ttgttcgccg gtcaagccgt ccggaagggc gcgggtgggt
     5401 ttgggggtaa ctgtttttat gccgtctgaa attttgccgt taagtaaaat ttcaatctgc
     5461 tcatcgcacc aaaccgcaaa tttcggatta agccaacggg caaagtgaat agcgagtttg
     5521 ggatgcagcc atgtgccttg ctcactgcca ccacgcttca cgataactat ttgatttgct
     5581 tccgttagga tttttctcct aacgcttaaa ttctcagcaa gtgcagagat atattgttga
     5641 gtttgttcac tttttaggta gtctttaggt aacttgccaa agtgagatgc aatggcggtt
     5701 gcatttaaaa aaccgtcttg acggaaagat acaggggtgt taccaaaatt gagaacagat
     5761 acgttcatga tagtttccta gtttagtttc gaaagaccca aatgggtggt cgggaggttc
     5821 gaaaacctac tagaaaaggc cggcgttatt ccccttgcgg gtattgtatt ccgcaccctc
     5881 ccgaccatag gaaactagtt cggatatatc gaaacaaaca accaaacaag aaaacttatg
     5941 gacgtaaaaa attcacgctg acggggtaaa tgccgttcta gtagaggttt tcgacgcctc
     6001 gtgtttcgta atatatccaa tctttctatt ttctgcaagc ataaaaaagc cgccatgtgg
     6061 cggatagggt cgcggtcttc aacagcatgt aggatttagg aatgcccaaa ttgggcgcag
     6121 gggtgttgaa aacacgcaaa caggcgcgcc gccagcctta cgggtaggcg cacccccaca
     6181 taggagtaaa tcgatttgat acgtatagac gtaaaaaatt ccactctatc gtgttggata
     6241 tacgctgttt gtcaggtgtt ttcaagcacc gtgggaaata ttatatagag ttatggaaat
     6301 gtgtcaagag aattagccca aatgggcgga cacgcggtta agaacccaca taggagggcg
     6361 gacttattcc cctttcgggt cttgtattcg tcgcccacgc ggtcatagaa acttcctgct
     6421 atcgaaacaa acaacaagga aagaaaacta tagacatgaa aaaatcacat tgacggagtg
     6481 attgccgctg tagtgtggtt tctgacgcca cgaacaggaa tataaaacaa aaccccctgc
     6541 acatgcaagg ggtttcccaa aaaaccgtag gcggcaaact gaaaggccgt ctgaatttca
     6601 gacggcctat gttgcggcgg atgttaagct attaagctat ttggttttca gaggcttttt
     6661 cttccatttt cgggctacgg cttcaaattc ttgaaaggcc gtcggtgcgc tcctgatacg
     6721 gcgtatttca aaaataaagc tgcttaggtt gtcccagtca cgtaacagca tggagtattc
     6781 gaaatcttta tacattttct cgtgcagtgc tccgcctaat acgcccgcac agacaaattc
     6841 gcgctggttt aaaactgtca gtatggcttc acggtctttc ttcttgtccg gggtatccga
     6901 tgtataggtg gcgagtatgc atccgtctgt ttttgccagc ccgtttacta tggttatggc
     6961 ttcttgaagg gcggcattgt tacgttcggc catgatcagc gcgctttttg gcgttttctg
     7021 tattcctcat gatgccgtaa gcggcaacaa aaacgccgat aacagtcaat atcggcgttg
     7081 ctatctgtat taggttgtca gtcattgccg tcccagccgt ctgaaaggcg gaaatcacgg
     7141 ctttccgaat aaatatgtcc cattgtcatc cccttaagct gatgttgaaa ataaatcctt
     7201 gcgtcggaat atacggcatt gcttgactat tttcaagatt ctgtacctcg tgaaccggac
     7261 tatacaaaaa aaccgccttc gtgtaaagcg gttcttaaat cgcccgttaa ggcggacaca
     7321 acgatagcca aactggaaca ggaggcggca aaggcgggtt tgtccaaaac agccttgatt
     7381 gaaaggctga ttggggattt gtaggcgttt gcagatacga caaggggcgc attccgcgcc
     7441 ccatcacacg cacggacggc ttgtcatcgc ctgtcgcccg tgcgcggctg cccatatttc
     7501 agaacagtct tgcaagcccc tttaagggaa cggttttatt ctagtacagt ttgaatgcct
     7561 tggcaacggc tgttttaatc gcctgaaaat cctcttcact gattattgga atgcagcggt
     7621 cgcgcccttt gggtttgtat cggtctaatc gtgccaatcc gactgttgcc gtcatgtcgc
     7681 attttgccca acattggatg tgcggcttgt ccggtaaggg gtttccactc attttgtggt
     7741 ggtagtccgc caaagggaca ggttctgtgc tgcttaaggg tacgaccgtt accagtttgc
     7801 cgttgtgcct gtttcgcgct atgacgacga cagggcgttt cttgaccatt tccggttctt
     7861 cataaccgcg aaagtcgcac atgataaccg aacgttccct tggttggaat tttaaaggca
     7921 ttagccgctc ctgacgatga cgggctgcta ttatagcagt tcttacacaa aaaaccgcct
     7981 ttgtgtaaag cggttgcaaa aaagccttcc aataaaaatg cggaaacggt tttttatttg
     8041 cgtttccgca taatactatc caccttcaga acctcgcgca aataagtacg cgcaagcgag
     8101 cgcaaaccgt gcgccgtcgt atccagcccc gcccgctgca tcgccacgcg cggactttcc
     8161 ttattgatat gcccttgaaa attcacactt tcaaacacaa accgcccgtt cacattcaac
     8221 gcccgcgcct gatccaacac ccactgcatc gcccgcgaca gcggcacatc atgcccccgc
     8281 cgctcctttt tccgttcggc ggggatacgc caaacctccc cgtccaactc cgaccactcc
     8341 atcaacgccg cctcctgaat ccgcgtcatt gtcaacagca gccaatagat acaaagccgc
     8401 gttacaggat ggatacgcac cctgcccgca taaaccgcaa attcattgcg ctgctccaaa
     8461 aaatcaatca ggcgcggcaa ttcagacgga ctcaacgctg ccatattccc cgttttcgcc
     8521 cgttcaaaca cacccgaacc gatttgcgcg acagggttga tttccatcat tcccgaaccg
     8581 accgcaaacg caaacaccat cttcagactg tttttcgttt tgcgcaacgt atcaacgata
     8641 ccgcgcgcct ccatcacacg cagacagccg accacatccg ccgtcctgat ttgacaaata
     8701 tcaagattgc cgatagccgg aaaaacccac cgctcaaaat tccgcataac ctgtccggca
     8761 tacttttcag accgcccctt cgaccaacgc acaaaccaat cacgcgccac cttctcaaaa
     8821 gcaaaatccg cccgcacctt cttattgacg acattttccc cgtgcgcccg ttttcggcgc
     8881 acctcctccc gccattcccg cgcatcggcc agcgaaaaat caggataccg ccccagcgaa
     8941 attgtctgct gccttccatc ctgcacaaac gacagcttcc aactccgccc gcccgtcggg
     9001 tagacccaca aagccagccc gccccgtctg acaacttata cagcttatcg cgcggcttcg
     9061 catttttaac ctgatttgcc gacagcggcg taatgatttt cgccattatg gtaatttccc
     9121 atttatccaa aaattaccat acacattacc ataaaaaaat gcaataagaa gatgccgcaa
     9181 gattgcaaat gatttgcttt gatgcgttat gatagggcaa accgatgatt tttatatggt
     9241 aattgataca acttgaacaa aaaagaaccg ccccgaatca gggcggtttt gttttgtggc
     9301 ggaaacggtg ggattcgaac ccacggagga tttgcaccct cagcggattt cgagtccgct
     9361 gcattcagcc tctctgccac gtttccgata atgcagtaaa accaaataaa aatacaatat
     9421 ttgcggctat tgcattctta tttggtttta cgctcaaaaa ttggcggaag cggtgagatt
     9481 cgaactcacg gagggctatc aaccctcgac ggttttcaag accgttgcat taaaccactc
     9541 tgccacgctt ccgtcttctt gaagattagg aataataatg aaatttatac attttgccaa
     9601 gcactttttt cggaaaatac ttaatttatt gttttcactt tatttgttcc gaacgcataa
     9661 aaacccattc gctttcattt gaagccgcgt cattgaatgc gtagccttcg taattaaaat
     9721 ttttcagcat ttcagggtcg gtaatatgat gttctgccgc atagcgcacc attaatccac
     9781 gcgcgcgctt ggcatagaaa ctgatgattt tatatttacc gtttttttcg tctttaaata
     9841 ttggggtaat cagccgcgcc cgaagttttt tcgtgttgac ggacttgaaa tattcctgtg
     9901 aggcaaggtt gacaagcgta ttgctgcctg cttgggcaag cgtatcattt aaaaggttgg
     9961 taatgatgtc gccccaaaac tcatacaaat tcttgccgcg caaattggca aatgccgtcc
    10021 ccatttccaa acgatagggc tgtatcaggt ctaacgggcg aagaagaccg tacagaccgg
    10081 acagcaggcg gacatggttt tgcagatagc gtatctgtcc aatatccaat gtgtttgcat
    10141 ccataccttc gtaaacatcg ccgttgaaca taaagaccgc ctgtttggcg ttttccggcg
    10201 taaacggcgt gttccattct gcattgcgct gcgcgtttaa gagggcaatt ttgtcggaaa
    10261 cgtgcatcag ttcggcaatc tgttgcggcg caagctcgcg caactgctgc attagaatgt
    10321 cggactctgc cagcaggtcg ggttgggtaa actcgctgac aggggcaggg tctttttcat
    10381 taaggttctt cgcaggggaa aggacaaaaa acataatctg ctcggtattg tgtcaaacac
    10441 ggggatttta agtttaaacg tgtttgcggg caagccgatt aaaagtgaag tatagtggat
    10501 taaatttaaa ccagtacagc gttgcctcgc cttgccgtac tatctgtact gtcttcggct
    10561 tcgtcgcctt gtcctgattt ttgttaatcc actataaata ccgttatagc aaattcaacc
    10621 gtaaaatatt gaaaaatgcc gtctgaacaa aagttcagac ggcatttttt tattctttac
    10681 cgatataggg aaacaatggt ttccgcagct ttgacacaag ttgcgacatc ggcaaccaaa
    10741 gcgatacgca cataaccttc cccgggattg ccctgttcgg tatcccgcgc caaaaaacgt
    10801 ccgggcaata cttggatagc cgctttttgc cataaattgc gtgcaagtgc caaatcgtcg
    10861 ccatcaggga ctttcaacca gatgtaaaac gaggcatccg gtaatttaac gtcaaatacc
    10921 tgttgcaaaa tgggaataac gcgctcaaat ttttcctgat acatacggcg gttgtcgata
    10981 acgtgctgtt catcatccca agcggcaatg cttgcgcgct gcacgggaat actcattgca
    11041 ctgccgtgat aggttctgta aagcagaaag tttttaagca gttcggcatc gccggcgaca
    11101 aaaccggaac gcaggcccgg aacgttggaa cgcttggaca aactggtgaa cataagcagt
    11161 ttttgcctgc ttcgacccaa ctgtgcagcg gcttgcaggc agcccaaagg tttgttgccg
    11221 tcgaaataga tttcggaata gcattcatcc gaggcaataa tgaaaccata tttatcctgt
    11281 aaatcaaaaa cttctttcca gccgtccaaa tccagcacgc tgccgctggg gttgttgggc
    11341 gagcagacga acaccagttt ggtgcgtttc caaacctctt cggaaatact gcgccaatcg
    11401 gggttgaaag acggcgcggg gcaattggca aaatggattt caccgccgcc caaaagtgtc
    11461 gcaccttcgt aaatctgata aaagggattc gggctgacaa ttgcgggttt gatgccgtct
    11521 gaaacagggt tcaacacggt ttgaacaaaa gaaaacaacg cctccctact gcctaaaacc
    11581 ggcagaattt cattatccgc atccactgtc aagccatcgt aacggcgttt taaccagttt
    11641 gcacacgcct gacgcagttc aggcagaccg gccgtcagcg gatatttttc caactcgtgc
    11701 aatgaggcgg tcagcgcatc cgtaatgact ttcggtgtcg gatgtttcgg ttcgccaatg
    11761 tgcaggggga cggcttccag accttcgggc gcggaaatgc cctgcatcgc ttcacgcagt
    11821 cgggcaaagg gatatggttt gagttggttg agtaaggtat tcatgatttt ctccggttgt
    11881 cggtatatcg gaagatatgc tgtctgaacc tgacgtgtct ttcagacggc attctatatt
    11941 tttagtgaga acccgtactg ccgaagccgc cctcaccccg gctgcttccg acaaactcct
    12001 cgacacgttt gaagcccgcc tgcacgattg gcacgacaac catctgcgcg atacgctcaa
    12061 acggtttgac agtaaacggt tcgctgcttc tgttccataa cgacaccttc aattcccctt
    12121 gataatcgga gtcaatcaaa ccgaccaaat tgcccaaaac aatgccgtgt ttatgcccca
    12181 agccggaacg gggcagcaaa acggcggcat atgcgggatt cgccaaataa attgccaaac
    12241 ccgtcggcac aagaaacgtt tcacccggct gcaaaacgac ttcctcatcc aaacaggcgc
    12301 gcaaatctaa acctgcagaa ccctccgttg catagacagg gacaacatcc gccatccgtt
    12361 cgtccaatac tttcatttct acttcaatat tcatcgtctt gctcctgcat acggccggtt
    12421 tcaaagatgg gagattatac acaagccgtg tttacgctga aacacggcaa atcggttttg
    12481 ttttgaaatc cgttgccagt gtataattcc cgcccttccg gttatgaaga cgaacattat
    12541 gcacaccttc cccgcataac gaatttatac gcggcatcaa agaaagttcg cctatgctga
    12601 ttgggctgct gccttgggca ttaatactcg gtatgcaggg cggacaaaaa ggcatgagct
    12661 ggctggaaat gttgttgatg accagtatga acttcgccgg cggctccgag tttgccacgg
    12721 tcaacctgtg ggcggaacct ctgccgatac tgcttatcgc caccgtaacc tttatgatta
    12781 attctcggca tatcctgatg ggggcggcgc ttgccccgca cctgaaagga ataccgctga
    12841 aaaaagccgt gcccgcactg ttttttatgt gtgatgaaag ctgggcgatg gcattctccg
    12901 aaatccaaaa acggaaagca gccggtttgc ccgcattcaa tatgcctttt tatagtggat
    12961 taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa gaaaacgatt ctctaaggtg
    13021 ctgaagcacc aagtgaatcg attccgtact atctgtactg tctgcagctt cgccgccttg
    13081 tcctgatttt tgttaatcca ctatatga
//
LOCUS       DAWIVB010000038        12761 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWIVB010000038 DAWIVB010000000
VERSION     DAWIVB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.038"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..325
                     /locus_tag="WIX60_000802"
     CDS             <1..325
                     /locus_tag="WIX60_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T cell stimulation protein TspA"
                     /protein_id="HEZ5305093.1"
                     /translation="TEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTGFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(498..914)
                     /gene="gloA"
                     /locus_tag="WIX60_000803"
     CDS             complement(498..914)
                     /gene="gloA"
                     /locus_tag="WIX60_000803"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ5305094.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WIX60_000804"
     CDS             complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WIX60_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ5305095.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2119..2433)
                     /locus_tag="WIX60_000805"
     CDS             complement(2119..2433)
                     /locus_tag="WIX60_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ5305096.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            2743..3741
                     /gene="ilvE"
                     /locus_tag="WIX60_000806"
     CDS             2743..3741
                     /gene="ilvE"
                     /locus_tag="WIX60_000806"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ5305097.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYSSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3985..4770)
                     /gene="fabI"
                     /locus_tag="WIX60_000807"
     CDS             complement(3985..4770)
                     /gene="fabI"
                     /locus_tag="WIX60_000807"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ5305098.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4925..5746)
                     /gene="dapD"
                     /locus_tag="WIX60_000808"
     CDS             complement(4925..5746)
                     /gene="dapD"
                     /locus_tag="WIX60_000808"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ5305099.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5756..5986)
                     /locus_tag="WIX60_000809"
     CDS             complement(5756..5986)
                     /locus_tag="WIX60_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305100.1"
                     /translation="MPSERRRRGIKVLPPFCIDSGAEKAIPAACPIPKRQCLATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(5956..7599)
                     /gene="pgi"
                     /locus_tag="WIX60_000810"
     CDS             complement(5956..7599)
                     /gene="pgi"
                     /locus_tag="WIX60_000810"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ5305101.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALKFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLFNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDRHFFSTPTRHNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGREDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILIDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            7956..9188
                     /gene="pilG"
                     /locus_tag="WIX60_000811"
     CDS             7956..9188
                     /gene="pilG"
                     /locus_tag="WIX60_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ5305102.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9262..10122
                     /locus_tag="WIX60_000812"
     CDS             9262..10122
                     /locus_tag="WIX60_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ5305103.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10124..10756
                     /locus_tag="WIX60_000813"
     CDS             10124..10756
                     /locus_tag="WIX60_000813"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ5305104.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            10749..10958
                     /locus_tag="WIX60_000814"
     CDS             10749..10958
                     /locus_tag="WIX60_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ5305105.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(11043..12719)
                     /gene="pilB"
                     /locus_tag="WIX60_000815"
     CDS             complement(11043..12719)
                     /gene="pilB"
                     /locus_tag="WIX60_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ5305106.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         2932 a         3551 c         3325 g         2953 t
ORIGIN      
        1 aacggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccggtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa ccggcacagg
      421 ctcaaacagc aggtcgacgg caacaaaatg ccgtctgaaa cccctaaagg cttcagacgg
      481 cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcgcc gcttttcttt
      541 tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac ggttgtgccg
      601 tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac acgttcgcag
      661 gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc caagtcgtat
      721 cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc atcgccgtaa
      781 ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag cagtttcata
      841 cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg gagcatagta
      901 tggagtaagc gcattttttg tgttcctttc ggtggtggtt aaacttcgat tttattcggg
      961 gtaaacgtct gccatttgtt gcaggcgggg cagtgccaga aaaagacttg tgatttgaag
     1021 tggcagtttc ggcaccggta catcacgctg cgctgtagct gccgtccgat aaccgaacgc
     1081 atcatatcgg catcggcttt ccaagccgga tccaaatcgc tgagtttcaa accaagcagg
     1141 cggtacacgc cgttgaggtc gggcttgcgg cggacaagct cgacggcggt ttgcgcggct
     1201 tctttctcgc acttaagcag cagggatttc tcgtacacga cattgatcag gtcaagttcg
     1261 ggaaacgtct gcatatatcc tgtcagacgg ttcaagcctt cttcaggttt tccctgcgcg
     1321 gcataggctt cgtaaagctt ctcgccgacc atgctcaagt atgcatggtt ttgctgctcg
     1381 atggcggcat aggcttcgac ggcggcaggg aaattgcctt gtcggtgttc gatgtcgccc
     1441 aaaatcatgt tggcacgggt gcattttttg ttggcttcga gtgccttgcc gacattgaaa
     1501 cgcgcgacat cgaaattgga cttgaacagc gcggcttggg caagttcgca ataaaactgg
     1561 gcgatttcaa actgataggt ctgatcgtca tggctgagca gccgggcggt ttcaaccgct
     1621 ttttcccaat ccctgtcctg ttggtagata ttgagcaggt gctgtctggc ttcacgcgcc
     1681 attttaccgt cttgcagccc caaaaaaatc tgttcggcac gatcgaccaa ccccgcactt
     1741 tggtagtttt gcgccaattc aaacaggacg cgcgcgcgct tttcgccgac cgtatcggga
     1801 gaatcgagca ttgtccggtg tatgttgatg gctttgtcgt tttcgccacg ctgacggtaa
     1861 agcttgccta gggtgaggtt taggtcgtat gactgaggct gctgatcaat gacctctgcc
     1921 aattccctgg cggcacgtcc gctgttgcgg tcaaccaagg catccagact tttataaaat
     1981 cccgacggta tgctttttgc ctgctttaat acagtcttca tatccacacg ggcggcaaac
     2041 cagcccatcg cgaagaaaac gggcaaaagg ataatcggca gcaggataat ccacaattca
     2101 ttgtccatat cggctttctc aaggctgttt ggcagattcg ggcgcatttt gcgccggtgg
     2161 tgcggtcagc tctttccccg tcaaacgcgc atttttcttt acttcggcac gcaacctgcc
     2221 gttctcgcca cgtaacgaca acaaccgtcc gaacaaggca aacattccga acacgatgcc
     2281 gacgacaaac gcgccgaaca atacgacaat cagcggcaaa tcgactttct gtccgggcag
     2341 gtaggagaag gtaacggcat ccgtattaat gacggcaagc agcaggaaga gcagcaggat
     2401 aatgattttg atgacggtat agataagttt catgtttgct ccgtaaataa aaacgccgcc
     2461 cggacagctt cgcactctga accaagattg cgctagttta aacgatttgc acggtttcgg
     2521 ataggatgcg gcagcgtgtt cggacgacat acggagtatg cgattgccga caattttacc
     2581 aaatacaccg ttcctttcca tttgaaaaat aacggattgg acaccgcatc gacagaaaaa
     2641 cccgccgcgc acttgtcaaa accctgtttg caggcgtatc tttacaatct tcaaattcaa
     2701 accgttcatt gaaacatatc agaataagaa aggctttaca tcatgagcag acccgtaccc
     2761 gccgtattcg gcagcgtttt tcacagtcaa atgcccgtcc tcgcctaccg cgaaggcaaa
     2821 tggcagccga ccgaatggca atcttcccaa gacctctccc tcgcaccggg tgcgcacgcc
     2881 ctgcactaca gcagcgaatg tttcgaggga ctgaaagcct tccgtcaggc agacggcaaa
     2941 atcgtactgt tccgtccgac tgccaacatc gcgcgtatgc ggcaaagtgc ggacattttg
     3001 cacctgccgc gccccgaaac ccaagcctat ctcgacgcgc taatcgaatt ggtcaaacgc
     3061 gccgccgatg aaattcccga tgcgcctgcc gccctgtacc tgcgtccgac cttaatcggt
     3121 accgatcccg tcatcggcaa ggccggttct ccttccgaaa ccgccctgct gtatattttg
     3181 gcttcccccg tcggcgacta tttcaaagtc ggatcgcccg tcaaaatttt ggtggaaacc
     3241 gaacacatcc gctgcgcccc gcatatgggc cgcgtcaaat gcggcggcaa ctacgcttcc
     3301 gccatgcact gggtgctgaa ggcgaaagcc gaatatggcg caaatcaagt cctgttctgc
     3361 ccgaacggcg acgtgcagga aaccggcgcg tccaacttta tcctgattaa cggcgatgaa
     3421 atcattacca aaccgctgac cgacgagttt ttgcacggcg taacccgcga ttccgtactg
     3481 acggttgcca aagatttggg ctataccgtc agcgaacgca atttcacggt tgacgaactc
     3541 aaagctgcgg tggaaaacgg tgcggaagcc attttgaccg gtacggcagc cgtcatctcg
     3601 cccgttactt ccttcgtcat cggcggcaaa gaaatcgaag tgaaaagcca agaacgcggc
     3661 tatgccatcc gtaaggcgat taccgacatc cagtatggtt tggcggaaga caaatacggc
     3721 tggctggttg aagtgtgctg atgctttaaa taaaaaatgc cgtctgaaac ccgtttggcg
     3781 tttcagacgg cattttcgca tccgaaccgt ttccgctgca cctgcagcaa gtcggcacaa
     3841 aggcaatcgg ttaaaacaag cgtccgcatt tcccatcccg cctgccgtaa gtcgggcatt
     3901 tccctagaaa tacgcttcag acggcaaaac gccgcccgaa accgatatgc ggcacggacg
     3961 gcgcacggat ttgaaaacgg cggattatcc ctcggtgctc aaggcattaa tgctgtaacc
     4021 gccgtcaacg taagtgattt cgccggtaat gccggacgac aggtcggaca gcaggaaggc
     4081 ggcggtattg ccgacttctt caatggtaac gttgcggcgg agcgggttgt gggcggcgac
     4141 gtgtcccaag agtttgccga aatcggcgat gccggaggcg gcaagcgttt taatcgggcc
     4201 ggcggaaata ccgttgcagc ggatgccctc tttacccaga caggcagcgg taaagcggat
     4261 gcctgcctca aggctggctt ttgccatacc catcacgttg taattcggaa tcgcgcgcac
     4321 cgcgcccaag tagctcaggg cgacgatggc ggaatttctg ccgcgcatca tcggacgggc
     4381 ggcttttgcc aacgcgggca ggctgtatgc ggaaatttcg tgtgcggtgt tgaacgcttc
     4441 gcggctgatg ctgtcgagga agtcgccgct caaggcttct ttcggcgcaa aaccgatgga
     4501 atgcaccaaa ccgtccaagc cgtcccaatg tttgcccaag tcggcgaaca cttggttgat
     4561 ttcgtcgtcg ctggcgacat cgcagcggaa tacaagttcg gaatccaatt ccgccgccat
     4621 tttgcggacg cgctcttcca gtttgtccac aacgtaggta aacgccagtt ccgcgccttg
     4681 ttcgcggcag gctttggcga tgccgtaagc gatggaacgc tcggaaatca tgccggtaat
     4741 cagaattttt ttgccttgca gaaaacccat tttgtatcct tcaaatagtg ttcggttcgt
     4801 tctaaaccgt tgattatagc aaattgtccc tgtttctgtg ttttcacgtt gcagcgtgca
     4861 aacggcaatg ccgtctgaag cggatttcag acggcattgg acgtttcaaa tacggtttaa
     4921 ggcatcagat gccgcgcaac aattcgttga cgctggtttt cgcacgggtt tgcgcgtcca
     4981 cgcgtttgac gatgacggcg cagtaaaggc tgtggctgcc gtctttggaa ggcatactgc
     5041 cggatacgac aaccgaacct gccggtacgc ggccttgata gatttcgccg gttgtacggt
     5101 caaagatttt ggtggattga ccgatgaaca cgcccataga aatcacgctg ccttcttcga
     5161 caatcacgcc ctcaacgatt tcagaacgcg caccgatgaa gcagttgtct tcaatgatgg
     5221 tgggtgcggc ctgcaggggt tcgagtacac caccgatgcc gacgcccccg ctcaagtgca
     5281 cgtttttacc gatttgcgcg caagagccga cggttgccca agtatcgacc atcgcgcctt
     5341 cgtcgacgta tgcgccgatg ttgacataag atggcatcag cacgacattt ttcgccacaa
     5401 agctgccgcg tcgggcaacc gcacccggaa ctgcgcggaa gcctgcgttt ttgaactcgt
     5461 cttcagacca gtcggcaaac ttggtcggca ctttgtcgaa gtatttgttc acgccgtcgt
     5521 tgaggacttc gttgtcttgg atgcggaagg acagcaacac ggcttttttc gcccattcgt
     5581 tgactttcca ctcacccacg cccaaacgtt cggcaacgcg cagtttgccg gaatcgagtt
     5641 ggcggatggt ttccaacacg gcttctttga cttcgggagt aacggtggtc ggggtgatgt
     5701 ccgcgcggtt ttcaaaggcg gtttcgataa tgttttgcaa agacataata tttccttatg
     5761 tgagatgttt cgtgggacgc ttgccggtat tttcaagcag tatgttcatt tgaaatgaat
     5821 gcgctattgt gccacgcaaa cgcatattcg tcagcaatac gcgagcggtt gccaaacatt
     5881 ggcgtttcgg aatcgggcag gcggcaggta ttgccttttc cgcgcccgaa tcaatacaga
     5941 aaggcggcag tacttttatg ccgcgccgcc gcctttcaga cggcattcgc ggtaaaacgc
     6001 catcagccct tcggtcgagg catcgtgtac ggacgtgccg ccttccagtt cgccgatgat
     6061 ggtttttgcc aactgtttgc cgtattccac cccccactga tcgaaggggt tgacgttcca
     6121 tatcgcgcct tggacgaagg ttttgtgttc gtaagccgcc atcagcatac ccaaattgta
     6181 gggcgtgagg cggtcaatca aaatgctgtt gctggggcgg ttgccgggga actctttgtg
     6241 cggcgcgagg cgttcgcgtt ccgcttcggg caaatctgcc agttcggcgc gtgcttcgtc
     6301 caaggttttg cccttcatca aggcttccgc ttgggcaaag gcgttggcaa cggtaaaacg
     6361 gctgcgtccg tcctctctgc cctgcgccgt catcgggacg ataaaatcgc agggaatcag
     6421 gcgcgtgcct tggtggagca gttggaaata ggcgtgctgg cagttgaccc cttcaccacc
     6481 gaacacgatg ccgcccgttt tgcacacggc gggactgccg tctgaagcgc ggcttttgcc
     6541 caaactctcc atatcgagct ggttcagcca cgccggcagc aggcgcaggt tgtggctgta
     6601 cggaacggcg gtctgcccgt ccgcgtgctg gaaattgttg taccacacgg caatcagtgc
     6661 cattaaaacg gggatattat gacgcgtcgg cgtactgaaa aaatgcctgt ccatcgcgtg
     6721 cgcccccgcc aacaactcgc ggaaacgcgc cccgccgacc gcaaccatca cgggcaaacc
     6781 gacgggcgac cagacggaat agcgtccgcc cacccagtcg tacatcgcaa acacgcgttc
     6841 cgccgcgata ccaaaagccg cagctgccgc agtgtcggca gacaccgcgc aaaaatggca
     6901 cgccgtttcg gattccgaga accctgcacc gcgataccac gccttgactg cctgtgcatt
     6961 gaacagggtt tccggtgttt tgaaggactt gctggcaacg caaaacactg tcgtttcggg
     7021 gttcagacgg cataaaaccg catccaggca ggcaggatcg gcgttggcgg caaaatggac
     7081 ggtgatatgc cgtctgaacg gctcaagtgc ctgcacgcac attgccggcc cgaggtcgga
     7141 tccgcctatg ccgatgtgga caaaatccgt aatccgtttt ccggttatcc cctgatacga
     7201 accgtcgtcc aaactgtgtg caaacttcaa cgcacgattt aactcgcggc ggatttcggg
     7261 caacacgtcc ctgccgtcca cataaacggc atccgcaccg tcgggcaggc gcaaagccgt
     7321 atgcagcgcg gcacgcccct cgctgccgtt gactttcgca cccgtccgca aagcacgcat
     7381 tttcccttcc aaatccgccg cgtcggcaag attgcagagc agttgcagcg tatcttcgcc
     7441 caaacggttt ttgctgtaat cgaacaacat cccgtccaaa cgctcgtgca tacgctcaaa
     7501 ccggtccggt tcgcaggcaa agcggtcgcg caaaaggaca tgacgcgtat cctgataatg
     7561 gcgttcgagc gcataccatg cacgggtaaa agcattcatc tgttttcctt gatttttcag
     7621 aaccggatta aaatgtagca gaatgtagtt taacaaacgg cagcggcttt ggcgaatctc
     7681 cggaacaccg cccgccgcgt ccaatagcac cttatttcca tatggcggaa atgccctccg
     7741 gccggcattt gacccgcaac aatatcctgc aagatttatt gtgtacgcat aaatgccgga
     7801 cagccgccta aataaggtat aatcgcatcc gattctgtcc cgtttgccgc ccgcaggcgg
     7861 gatgtcggcg gttttcatct taccgggaac acgccggtac tgttttttaa cgatgtttct
     7921 tacattttat tccaattact ttacggggct agaatatggc taaaaacgga ggattttctt
     7981 tgttcgcaaa gaaagaaaaa cgctttatct ttgaaggcag gcattccgcc tccgacaaac
     8041 tggtcaacgg cgaagtatcc gcgtttaccg aagaagaggc gcgcaaaaaa ctggcaaaac
     8101 gcggcatccg cccgttgcag attacccgtg tgaaaacaag ctccaagcgc aaaatcacac
     8161 aagaagacat caccgttttc acccgccagc tgtccacgat gattaaagcg ggcctgccgc
     8221 tgatgcaggc atttgaaatc gtggcgcgcg gacacggcaa cccgtctatg acggaaatgc
     8281 tgatggaaat ccgaggcgaa gtggaacagg gcagctcgtt gagccgcgca ttctcaaacc
     8341 acccaaaata tttcgaccgc ttctactgca atctggttgc ggcgggcgaa acgggcggcg
     8401 tattggaaag cctgctggac aaattggcaa tttacaaaga aaaaacccag gccatccgca
     8461 aaaaggtaaa aaccgcactg acctatccgg tatccgtcat cgccgtcgcc atcggtttgg
     8521 tattcgtgat gatgattttc gtactgcccg cctttaaaga agtttacgcc aatatgggcg
     8581 cggagcttcc cgcactgacc caaacagtga tggatatgtc cgactttttc gtctcatacg
     8641 gctggatggt gctgatcgca ctgggctttg ccatatacgg cttccttaaa ttgaaggcgc
     8701 gttcgattaa aatccaacgg cgtatggatg ccatactgct gcgtatgccg attttcggag
     8761 acattgtccg caaaggaacg attgcccgct ggggcaggac gacggcgacg ctgattgcgg
     8821 caggcgtgcc tttggtcgat gtattggact ccactgccgg cgcggcgggc aatttaatct
     8881 atgaagaagc cacccgggaa atccgtacgc gcgtcatcca aggtttgtct atgacttcgg
     8941 ggatgcgtgc gacggaactg ttccccaata tgatgttgca gatgtcttcc atcggcgagg
     9001 aatcgggttc tttggacgat atgctcaaca aagccgccga attttacgaa gacgaggtgg
     9061 acaatgcggt cggcaggctg tccgctatga tggagccgat cattatcgtg attttaggct
     9121 tggtcatcgg cacgcttctg gtcgccatgt atctgccgct gtttaatttg ggcaacgtgg
     9181 tcgcctgatt tgccgcacag atccggcgcg gattcgctct gcgccggttt gtttttgttt
     9241 tgaatacatt gagaacaaaa tatgtctgat ttgtctgtat tgtcgccgtt tgccgtgcct
     9301 ttggcagcgg tgttcgggct gctggtcgga agtttcttaa atgtcgtcat ttaccgcgtg
     9361 ccggtcatga tggaacgcgg ctggacggta tttgccaaag aatatttaaa cctgccgctg
     9421 accgaagagg aaagccgtac cttcaacctg atgaaaccgg attcctgctg tcccaaatgc
     9481 cgcgtgccga tacgcgcgtg gcagaacatc ccgattgtca gctacctgct cctgcgcggc
     9541 aaatgcgctt cctgccaaac caaaatcagc atacgttatc ccttaatcga gctgctgacc
     9601 ggcgtattgt tcgggctggt cgcctggcaa tacggctggt cttggattac gctgggcgga
     9661 ttggtactga ccgcgtttct gatttccctg acctttatcg atgcggacac ccaatacctg
     9721 cccgactcga tgacactgcc cttaatttgg ctgggtctga tatttaattt ggacggcggc
     9781 ttcgtgcctt tgcagtctgc cgttttaggt gcggtcgccg gctatggttc attatggctc
     9841 ttatgtgcag tgtataaact gctcacagga aaaaccggta tgggcaacgg agatttcaaa
     9901 ctgattgccg cattgggcgc gtggctcggc atatccgcat tgcccgtact gatttttgtt
     9961 tcctcgctga tcggtttggt cgcggcaatc gttatgcgcg tcgccaaggg gcagcatttt
    10021 gccttcggcc ccgcactgac agtttcgggc tggataattt ttacggcaaa cgattccgta
    10081 tggcgggcgg tcaactggtg gctgacccat ccggtgctgt aagatgacgg tatgggtcgg
    10141 actgaccggc ggaatcggca gcggcaaatc ggcagccgcg caatgttttg ccgatttggg
    10201 cgtgccgcgc atcgatgcag acgcggcggc gcactcgctg acggcttcag acggcatcgc
    10261 cctgccggaa atcaggcggc tgttcggcga caccgttttt gacacacagg gtttgttgcg
    10321 gcgcgacata ttgcgtaaag aagtctttgc ctccccatcg cgaaaagcct tgctcgaatc
    10381 cgtgatgttg ccgctgattt tctcagaaat caaaaaacag caagaaacct ttactgatgc
    10441 agcttacggc attgtcgaaa ttccgctgct gacggaaaag cgtcaattta tcagcctgat
    10501 acggcgtgtc ctgaccataa gtgcccctgt ggaaaaacgt atcggcaggg tgatggcccg
    10561 cagcgggctg acgcgcggcg aggtggcggc cgtcatcagc catcaggcat ccgaatccga
    10621 acgcctgctg cttgcagacg atgtgctgct caatgacggc agcctcaaaa gcctgcgtga
    10681 gaaaacaatg cgcctgcacg cgttttattc agggattttc gcctcaaaac caacacaagg
    10741 aaaacacaat gactgaatcg cggcaaacac gccttcaagt caaatgtccg acctgtcaaa
    10801 cagcagtagt atggaaaccc gaaaacgcat tccgcccctt ctgttcgcaa cgctgcaaac
    10861 tgatcgactt gggcggatgg gcagacggga aatatacggt ttccggccaa acggaaagtt
    10921 tgccggaaat atccgaaccc gacatggcat accgctgacc gccccgcctt cccggcaaac
    10981 accctgaaag tcaaatgccg tctgaaacaa acacgcttca gacggcattt tcattctcaa
    11041 acctaatcgt tggtatttgc cgttacctct tccaatgaag taatgccctg cataactttc
    11101 aaaataccgg cccggcgcaa atccaccata ccctccttat aggcaacgtc caaaatatcc
    11161 acttccgtac cgttgttcat aatcacacgc tgcatttctt cgctgatggg cataacctca
    11221 tacacgcccg cacgcccctt ataaccctgc ccccggcaac ggtcgcaacc gacggcgcgg
    11281 taaagtttcc aatcttttgc aaggtcctca tcggtgaagc cgacttcctt caaagcagag
    11341 gcagacgggc gttccacttc ctgtttgcag ctcgaacaca gcctgcgtaa aagacgctgc
    11401 gccataatca ggctgaccga actggcaata ttaaacggcg cgacacccat attcagcata
    11461 cgcgacaacg tcgccggcgc attattggtg tgcagggtgg aaaacaccat atgccctgtt
    11521 tgtgccgcct taatcgcaat atcggcagtt tccaaatcac gaatctcacc gaccataatg
    11581 atgtccgggt cctgacgcag gaaagacttc aaagcagcgg caaaagtcag gccctgctta
    11641 tcattgacgt taacctgatt gatgcccggc aggttaatct cggcagggtc ttccgccgtt
    11701 gcaatattta ccgactccgt attcaaaata ttcaaacagg tatagagcga caccgtctta
    11761 cccgaacccg tcggaccggt taccagcacc atcccgtagg gacggtgaat cgcttccaac
    11821 aacaattttt tctgaaacgg ctcaaaaccg agctggtcga tgttcaaaga cgcggcatcg
    11881 gaattcaaaa tccgcatcac gaccttttcg ccaaacagcg tcggcaatgt gctgacacgg
    11941 aaatcgacag gcttgccgcc cttttgaaag gtcagctgca tcctgccgtc ctgcggtatc
    12001 cgtttttcgg aaatgtccaa acgcgacatt accttaatcc gtgaagcaag ctgccccctt
    12061 accgcaatgg gcggctgaac cacctcgcgg agctgcccgt ccacacggaa acggatacgg
    12121 gcattgtgtt cgtaaaactc gaaatggatg tccgatgccc cgctgcgcaa ggcatccgac
    12181 aaagtcttat ggataaacct cggaacaggg ccgtcttctg cctcctcgtt gtcgatatac
    12241 agggtgtggc tttcctcttc ctcctgcccc tccccaagct cctgaagcag cgatgtcgaa
    12301 cgcgaaccca cccaatcgag caaacccgcc aactggtcat cctcgacaat gaccaactca
    12361 acctcaatcc ctgcggcaga aacggttttc tgaatttgcg gcatctgtgt cggatcggaa
    12421 accgcaaaaa atactttgtc gccccgacgg aaaaccggca cacagtggaa ctccaccatc
    12481 tgctcctccg tcaacacccc catcagcacc ctgtggcgcg gataatgacg caaatcaaga
    12541 atcgaataac tgaacaccct cgcaatcaat gccgcaagcg acttgggcga aatgacaccg
    12601 tctgaaaaca gcatcggcaa cacttcctta cccgcctgcg actcattgta gtaatgctcg
    12661 gcctgctcaa cagtaaccac ctggttttga accagaatcc tcagcaaacc tacgctcata
    12721 cgaccttatc cccaaattta ttcattgtta tacctgtaca g
//
LOCUS       DAWIVB010000039        12371 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWIVB010000039 DAWIVB010000000
VERSION     DAWIVB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12371
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.039"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..1647
                     /gene="hrpB"
                     /locus_tag="WIX60_000816"
     CDS             <1..1647
                     /gene="hrpB"
                     /locus_tag="WIX60_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ5305107.1"
                     /translation="SMQRQQHIDAELLTDANVRFEQPLEKNNYVLSEDETPCTRVNYI
                     SLDDKTVRKFSFLPSVLMKETAFKTGMCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQ
                     PQNMDSGILKLRVSAGEIGDIRYEEKRDGKSAEGSISAFNNKFPLYRNKILNLRDVEQ
                     GLENLRRLPSVKTDIQIIPSEEEGKSDLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGN
                     VALSFDNPLGLSDLFYVSYGRGLAHKTDLTDATGTETESGSRSYSVHYSVPVKKWLFS
                     FNHNGHRYHEATEGYSVNYDYNGKQYQSSLAAERMLWRNRLHKTSVGMKLWTRQTYKY
                     IDDAEIEVQRRRSAGWEAELRHRAYLNRWQLDGKLSYKRGTGMRQSMPAPEENGGDIL
                     PGTSRMKIITAGLDAAAPFILGKQQFFYATAIQAQWNKTPLVAQDKLSIGSRYTVRGF
                     DGEQSLFGERGFYWQNTLTWYFHPNHQFYLGADYGRVSGESAQYVSGKQLMGAVVGFR
                     GGHKVGGMFAYDLFAGKPLHKPKGFQTTNTVYGFNLNYSF"
     gene            1756..3537
                     /locus_tag="WIX60_000817"
     CDS             1756..3537
                     /locus_tag="WIX60_000817"
                     /inference="COORDINATES: protein motif:HMM:NF017658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEZ5305108.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFVVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGPATLSADGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTN"
     gene            5041..7743
                     /locus_tag="WIX60_000818"
     CDS             5041..7743
                     /locus_tag="WIX60_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ5305109.1"
                     /translation="MNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKA
                     HSDIVLEAGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGG
                     NIEANTTRFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKN
                     ELNETKLPVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKG
                     IVNRIQSEEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLTAPGGYIVDIPKGN
                     LKTEIEKLAKQPEYAYLKQLQVAKNVNWNQVQLAYDKWDYKQEGLTRAGAAIVTIIVT
                     ALTYGYGATAAGGVAASGSSTAAAAGTAATTTAAATTVSTATAMQTAALASLYSQAAV
                     SIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTG
                     NQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIK
                     TTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVADSMLGGRNPATLSDAE
                     KHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDK
                     TALEKIIQGIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPL
                     IGTAGKLAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKAKAAK
                     QAAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAGLLDASNIPIT
                     INGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDGRLANGIISTG
                     EWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            7762..8136
                     /locus_tag="WIX60_000819"
     CDS             7762..8136
                     /locus_tag="WIX60_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ5305110.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDENLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            8495..10807
                     /locus_tag="WIX60_000820"
                     /pseudo
     CDS             8495..10807
                     /locus_tag="WIX60_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
     gene            10816..11193
                     /locus_tag="WIX60_000821"
     CDS             10816..11193
                     /locus_tag="WIX60_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305111.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            11471..11845
                     /locus_tag="WIX60_000822"
     CDS             11471..11845
                     /locus_tag="WIX60_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305112.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            11880..>12371
                     /locus_tag="WIX60_000823"
     CDS             11880..>12371
                     /locus_tag="WIX60_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ5305113.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGNIGNTLKELGRSSTVKNLMV
                     AVAT"
BASE COUNT         3759 a         2874 c         2934 g         2804 t
ORIGIN      
        1 tccatgcagc gtcagcagca catagatgct gaattgttaa ctgatgcaaa tgtccgtttc
       61 gagcaaccat tggagaagaa caattatgtc ctgagtgaag atgaaacacc gtgtactcgg
      121 gtaaattaca ttagtttaga tgataagacg gtgcgcaaat tttcttttct tccttctgtg
      181 ctcatgaaag aaacagcttt taaaactggg atgtgtttag gttccaataa tttgagcagg
      241 ctacaaaaag ccgcgcaaca gatactgatc gtgcgtggct acctcacttc ccaagctatt
      301 atccaaccac agaatatgga ttcgggaatt ctgaaattac gggtatcagc aggcgaaata
      361 ggggatatcc gctatgaaga aaaacgggat gggaagtctg ccgagggcag tattagtgca
      421 ttcaataaca aatttccctt atataggaac aaaattctca atcttcgcga tgtagagcag
      481 ggcttggaaa acctgcgtcg tttgccgagt gttaaaacag atattcagat tataccgtcc
      541 gaagaagaag gcaaaagcga tttacagatc aaatggcagc agaataaacc catacggttc
      601 agtatcggta tagatgatgc gggcggcaaa acgaccggca aatatcaagg aaatgtcgct
      661 ttatcgttcg ataacccttt gggcttaagc gatttgtttt atgtttcata tggacgcggt
      721 ttggcgcaca aaacggactt gactgatgcc accggtacgg aaactgaaag cggatccaga
      781 agttacagcg tgcattattc ggtgcccgta aaaaaatggc tgttttcttt taatcacaat
      841 ggacatcgtt accacgaagc aaccgaaggc tattccgtca attacgatta caacggcaaa
      901 caatatcaga gcagcctggc cgccgagcgc atgctttggc gtaacagact tcataaaact
      961 tcagtcggaa tgaaattatg gacacgccaa acctataaat acatcgacga tgccgaaatc
     1021 gaagtacaac gccgccgctc tgcaggctgg gaagccgaat tgcgccaccg tgcttacctc
     1081 aaccgttggc agcttgacgg caagttgtct tacaaacgcg ggaccggcat gcgccaaagt
     1141 atgcctgcac cggaagaaaa cggcggcgat attcttccag gtacatctcg tatgaaaatc
     1201 attactgccg gtttggacgc agccgcccca tttattttag gcaaacagca gtttttctac
     1261 gcaaccgcca ttcaagctca atggaacaaa acgccgttgg ttgcccaaga taaattgtca
     1321 atcggcagcc gctacaccgt tcgcggattt gatggggagc agagtctttt cggagagcga
     1381 ggtttctact ggcagaatac tttaacttgg tattttcatc cgaaccatca gttctatctc
     1441 ggtgcggact atggccgcgt atctggcgaa agtgcacaat atgtatcggg caagcagctg
     1501 atgggtgcag tggtcggctt cagaggaggg cataaagtag gcggtatgtt tgcttatgat
     1561 ctgtttgccg gcaagccgct tcataaaccc aaaggctttc agacgaccaa caccgtttac
     1621 ggcttcaact tgaattacag tttctaacct ctgaattttt tactgatatt tagacggtct
     1681 ttccttatcc tcagaccgtc aaactttacc tacgtacttg gcgcgcagta cgttcatctt
     1741 caaaatggaa tagacatgaa taaaggttta catcgcatta tctttagtaa aaagcacagc
     1801 accatggttg cagtagccga aactgccaac agccagggca aaggtaaaca ggcaggcagt
     1861 tcggtttctg tttcactgaa aacttcaggc gacctttgcg gcaaactcaa aaccaccctt
     1921 aaaactttgg tctgctcttt ggtttccctg agtatggtat tgcctgccca tgcccaaatt
     1981 accaccgaca aatcagcacc taaaaaccag caggtcgtta tccttaaaac caacactggt
     2041 gcccccttgg tgaatatcca aactccgaat ggacgcggat tgagccacaa ccgctatacg
     2101 cagtttgatg ttgacaacaa aggggcagtg ttaaacaacg accgtaacaa taatccgttt
     2161 gtggtcaaag gcagtgcgca attgattttg aacgaggtac gcggtacggc tagcaaactc
     2221 aacggcatcg ttaccgtagg cggtcaaaag gccgacgtga ttattgccaa ccccaacggc
     2281 attaccgtta atggcggcgg ctttaaaaat gtcggtcggg gcatcttaac taccggtgcg
     2341 ccccaaatcg gcaaagacgg tgcactgaca ggatttgatg tgcgtcaagg cacattgacc
     2401 gtaggagcag caggttggaa tgataaaggc ggagccgact acaccggggt acttgctcgt
     2461 gcagttgctt tgcaggggaa attacagggt aaaaacctgg cggtttctac cggtcctcag
     2521 aaagtagatt acgccagcgg cgaaatcagt gcaggtacgg cagcgggtac gaaaccgact
     2581 attgcccttg atactgccgc actgggcggt atgtacgccg acagcatcac actgattgcc
     2641 aatgaaaaag gcgtaggcgt caaaaatgcc ggcacactcg aagcggccaa gcaattgatt
     2701 gtgacttcgt caggccgcat tgaaaacagc ggccgcatcg ccaccactgc cgacggcacc
     2761 gaagcttcac cgacttatct ctccatcgaa accaccgaaa aaggagcggc aggcacattt
     2821 atctccaatg gtggtcggat cgagagcaaa ggcttattgg ttattgagac gggagaagat
     2881 atcagcttgc gtaacggagc cgtggtgcag aataacggca gtcgcccagc taccacggta
     2941 ttaaatgctg gtcataattt ggtgattgag agcaaaacta atgtgaacaa tgccaaaggc
     3001 ccggctactc tgtcggccga cggccgtacc gtcatcaagg aggccagtat tcagactggc
     3061 actaccgtat acagttccag caaaggcaac gccgaattag gcaataacac acgcattacc
     3121 ggggcagatg ttaccgtatt atccaacggc accatcagca gttccgccgt aatagatgcc
     3181 aaagacaccg cacacatcga agcaggcaaa ccgctttctt tggaagcttc aacagttacc
     3241 tccgatatcc gcttaaacgg aggcagtatc aagggcggca agcagcttgc tttactggca
     3301 gacgataaca ttactgccaa aactaccaat ctgaatactc ccggcaatct gtatgttcat
     3361 acaggtaaag atctgaattt gaatgttgat aaagatttgt ctgccgccag catccatttg
     3421 aaatcggata acgctgccca tattaccggc accagtaaaa ccctcactgc ctcaaaagac
     3481 atgggtgtgg aggcaggctc gctgaatgtt accaatacca atctgcgtac caactagggt
     3541 aatctgcaca ttcaggcagc caaaggcaat attcagcttc gcaataccaa gctgaacgca
     3601 gccaaggctc tcgaaaccac cgcattgcag ggcaatatcg tttcagacgg ccttcatgct
     3661 gtttctgcag acggtcatgt atccttattg gccaacggta atgccgactt taccggtcac
     3721 aataccctga cagccaaggc cgatgtcaat gcaggatcgg ttggtaaagg ccgtctgaaa
     3781 gcagacaata ccaatatcac ttcatcttca ggagatatta cgttggttgc cggcaacggt
     3841 attcagcttg gtgacggaaa acaacgcaat tcaatcaacg gaaaacacat cagcatcaaa
     3901 aacaacggtg gtaatgccga cttaaaaaac cttaacgtcc atgccaaaag cggggcattg
     3961 aacattcatt ccgaccgggc attgagcata gaaaatacca agctggagtc tacccataat
     4021 acgcatctta atgcacaaca cgagcgggta acgctcaacc aagtagatgc ctacgcacac
     4081 cgtcatctaa gcattaccgg cagccagatt tggcaaaacg acaaactgcc ttctgccaac
     4141 aagctggtgg ctaacggtgt attggcactc aatgcgcgct attcccaaat tgccgacaac
     4201 accacgctga gagcgggtgc aatcaacctt actgccggta ccgccctagt caagcgcggc
     4261 aacatcaatt ggagtaccgt ttcgaccaaa actttggaag ataatgccga attaaaacca
     4321 ttggccggac ggctgaatat tgaagcaggt agcggcacat taaccatcga acctgccaac
     4381 cgcatcagtg cgcataccga cctgagcatc aaaacaggcg gaaaattgct gttgtctgca
     4441 aaaggaggaa atgcaggtgc gcctagtgct caagtttcct cattggaagc aaaaggcaat
     4501 atccgtctgg ttacaggaga aacagattta agaggttcta aaattacagc cggtaaaaac
     4561 ttggttgtcg ccaccaccaa aggcaagttg aatatcgaag ccgtaaacaa ctcattcagc
     4621 aattattttc ctacacaaaa agcggctgaa ctcaaccaaa aatccaaaga attggaacag
     4681 cagattgcgc agttgaaaaa aagctcgcct aaaagcaagc tgattccaac cctgcaagaa
     4741 gaacgcgacc gtctcgcttt ctatattcaa gccatcaaca aggaagttaa aggtaaaaaa
     4801 cccaaaggca aagaatacct gcaagccaag ctttctgcac aaaatattga cttgatttcc
     4861 gcacaaggca tcgaaatcag cggttccgat attaccgctt ccaaaaaact gaaccttcac
     4921 gccgcaggcg tattgccaaa ggcagcagat tcagaggcgg ctgctattct gattgacggc
     4981 ataaccgacc aatatgaaat tggcaagccc acctacaaga gtcactacga caaagctgct
     5041 ctgaacaagc cttcacgttt gaccggacgt acaggggtaa gtattcatgc agctgcggca
     5101 ctcgatgatg cacgtattat tatcggtgca tccgaaatca aagctccctc aggcagcata
     5161 gacatcaaag cccatagtga tattgtactg gaggctggac aaaacgatgc ctataccttc
     5221 ttaaaaacca aaggtaaaag cggcaaaatc atcagaaaaa ccaagtttac cagcacccgc
     5281 gaccacctga ttatgccagc ccccgtcgag ctgaccgcca acggcataac gcttcaggca
     5341 ggcggcaaca tcgaagctaa taccacccgc ttcaatgccc ctgcaggtaa agttaccctg
     5401 gttgcgggtg aagagctgca actgctggca gaagaaggca tccacaagca cgagttggat
     5461 gtccaaaaaa gccgccgctt tatcggcatc aaggtaggca agagcaatta cagtaaaaac
     5521 gaactgaacg aaaccaaatt gcctgtccgc gtcgtcgccc aaactgcagc cacccgttca
     5581 ggctgggata ccgtgctcga aggtaccgaa ttcaaaacca cgctggccgg tgcggacatt
     5641 caggcaggtg taggcgaaaa agcccgtgcc gatgcgaaaa ttatcctcaa aggcattgtg
     5701 aaccgtatcc agtcggaaga aaaattagaa accaactcaa ccgtatggca gaaacaggcc
     5761 ggacgcggca gcactatcga aacgctgaaa ctgcccagct tcgaaagccc tactccgccc
     5821 aaactgaccg cccccggtgg ctatatcgtc gacattccga aaggcaattt gaaaaccgaa
     5881 atcgaaaagc tggccaaaca gcccgagtat gcctatctga aacagctcca agtagcgaaa
     5941 aacgtcaact ggaaccaggt gcaactggct tacgataaat gggactataa gcaggaaggc
     6001 ttaaccagag ccggtgcagc gattgttacc ataatcgtaa ccgcactgac ttatggatac
     6061 ggcgcaaccg cagcgggcgg tgtagccgct tcaggaagta gtacagccgc agctgccgga
     6121 acagccgcca caacgacagc agcagctact accgtttcta cagcgactgc catgcaaacc
     6181 gctgctttag cctccttgta tagccaagca gctgtatcca tcatcaataa taaaggtgat
     6241 gtcggcaaag cgttgaaaga tctcggcacc agtgatacgg tcaagcagat tgtcacttct
     6301 gccctgacgg cgggtgcatt aaatcagatg ggcgcagata ttgcccaatt gaacagcaag
     6361 gtaagaaccg aactgttcag cagtacgggc aatcaaacta ttgccaacct tggaggcaga
     6421 ctggctacca atctcagtaa tgcaggtatc tcagctggta tcaataccgc cgtcaacggc
     6481 ggcagcctga aagacaactt aggcaatgcc gcattaggag cattggttaa tagcttccaa
     6541 ggagaagccg ccagcaaaat caaaacaacc ttcagcgacg attatgttgc caaacagttc
     6601 gcccacgctt tggctgggtg tgttagcgga ttggtacaag gaaaatgtaa agacggggca
     6661 attggcgcag cagttgggga aatcgtagcc gactccatgc ttggcggcag aaaccctgct
     6721 acactcagcg atgcggaaaa gcataaggtt atcagttact cgaagattat tgccggcagc
     6781 gtggcggcac tcaacggcgg cgatgtgaat actgcggcga atgcggctga ggtggcggta
     6841 gtgaataatg ctttgaattt tgacagtacc cctaccaatg cgaaaaagca tcaaccgcag
     6901 aagcccgaca aaaccgcact ggaaaaaatt atccaaggta ttatgcctgc acatgcagca
     6961 ggtgcgatga ctaatccgca ggataaggat gctgccattt ggataagcaa tatccgtaat
     7021 ggcatcacag gcccgattgt gattaccagc tatggggttt atgctgcagg ttggacagct
     7081 ccgctgatcg gtacagcggg taaattagct atcagcacct gcatggctaa tccttctggt
     7141 tgtactgtca tggtcactca ggctgccgaa gcgggcgcgg gaatcgccac gggtgcggta
     7201 acggtaggca acgcttggga agcgcctgtg ggggcgttgt cgaaagcgaa ggcggctaag
     7261 caagctgctc ctaaagaaac aataaacaat ttggcaaatt tagccaaagc agaacagcag
     7321 attttattcc gtattgccca acgcgatacg caactggatg catggaagac gggatttaac
     7381 aatagagtaa ggaaaggagc aggcttgctt gatgcaagta atattccgat aaccattaac
     7441 ggaaaaacca tcaaacctgt acaagccata agcttaaagg gagcacccgt ttacagcggc
     7501 gtaagcgaac aggagatttt tgcgctttat cggcagatga ctggccagaa tccgaatttt
     7561 agagttttgc ctgacggaag attagcaaat ggcattatca gtactggaga atgggcagga
     7621 acaaaaattg cattaagaaa tttttcaaaa acagagaatt caactcaagc acgatggaca
     7681 ttagatttgc agaatcctcc atcatttatt aaaggtacta aattggagct taaattccaa
     7741 taatttacaa aggattttac cgtggatgag aaacaaaaaa ttaagattct tgattttcaa
     7801 atcgatttat cctcaatttt taactcttat aaaaatcaaa tgggtattaa tattcaagat
     7861 gaaaacttaa aaaacaattt ctgttctttt atggaagaac tgttaaatga cggttcaatc
     7921 cgtttacatg attataccga cggtatcgga attcctctaa ctggaacttc aaaagaacaa
     7981 gtgcagaaat tgaaagacat atggcctact ttggaagatg cccaagcaat atggcctgaa
     8041 gacccttggt attacttaga atggctttgg tgggatattg cgtgtccaat agatttggcc
     8101 gatttgccga atattgatat ttatgagcaa gcgtaggtat ggttagccgc ctttagcggc
     8161 gtaaccgtac gcatatcagc aaactttata aaataacaag gccgtctgaa atctgttttt
     8221 caactttttc agacggcctt gcaacttggc atttcattcg tacggttacg ccgctaaagg
     8281 cggctaaccg tacctacgag ctctgataaa aatgatttat ggaagcaagc tgtagcctgc
     8341 atgaaaccta aaatccatgc gtaaggtgtg tgcttcagcg cgcacgcgtt ccatgattta
     8401 cggctcaatg ccgtctgaaa agctcaattt tttcagacgg catttgttat gcaagtaaat
     8461 attcagattc tctgtatact gttcagacgc gtgcgtgctg aagacacctc ctacgcttgc
     8521 tgcagaactt tcgggtaaaa ccggtgtgag cattagcgcg ccgtatgcca atgaaaacag
     8581 ccgcatcctg ctgagcacca cggatatcag ttcggaaaac ggcaaaatca aactgcaatc
     8641 ctacggcgac cagttctact acgccggaca gggtgagctc tacaccttcg ataaacgcag
     8701 ctataaaacc ggtaagtggt acaaactaaa acatgttact gaaatcaaag agcataaaaa
     8761 cgccaaagcc gacccggtga gcctcagtgc gtcacaaggt attgaaatca aatccggcgg
     8821 caatatcggt gcccacgcca ccttgtttga tgcaccccgc ggctccgtta aaatcgaagc
     8881 cggacgtggg ctggttctct atgccgtgga agatctcaac tacgacaaac ttgacacccg
     8941 taccaagcgc aaatttatcg gcattaccta cgacaaggtg cacgacacca ccacccacac
     9001 catgaaaacc gccctgccct caagggtagt tgcagaatag gccaacctgc aatcaggctg
     9061 ggacgccaaa ctgcaaggca cccagtttga aaccacgctg ggcggcgcag ccatccgtgc
     9121 aggtgtaggc gatcaggcac gagcagatgc caagattatt cttgaaggca tcaaaagtag
     9181 tgtgcgcact gaaacagtaa gcagtagcaa atctgccctc tggcagaaac aggccggacg
     9241 cggcagcaat atcgaaacct tgcaactgcc aagtttcaca ggctccgttg cgcccgtact
     9301 ctctgccccc ggcggctata tcgttgatat tccgaaaggc aatctgaaaa ccgaaatcga
     9361 aaagctggcc aaacagcccg agtatgccta tctgaaacag ctccaagtag cgaaaaacgt
     9421 caactggaac caggtgcaac tggcttacga taaatgggac tataagcagg aaggcttaac
     9481 cagagccggt gcagcgatta tcgcgctggc tgttaccgtg gttactgcgg gcgcgggagt
     9541 cggagccgca ctaggcttaa acggcgcagc cgcagcagcg gccgatgccg cctttgcctc
     9601 actcgcttct caggcttccg tatcgctcat caacaataaa ggcgatgtcg gcaaaaccct
     9661 gaaggaactg ggcagaagcc gcacggtaaa aaatctggtt gtagcggcgg caacggcagg
     9721 cgtatccaac aaactcggtg cctcttccct tgccacttgg agcgaaaccc cttgggtaaa
     9781 caacctcaac gttaacctgg ccaatgcggg cagtgccgcg ctgatcaaca ccgctgttaa
     9841 cggcggcagc ctgaaagaca atctggaggc aaatatcctg gcggcattgg tgaataccgc
     9901 gcatggggag gcggcgagta agatcaaagg actggatcag cactatgtcg cccacaaaat
     9961 cgctcatgcc gtagcgggct gtgcggctgc agcggcgaat aagggcaaat gtcaggacgg
    10021 cgcgatcggt gcggctgtgg gtgagattgt cggggaggct ttggttaaaa ataccgattt
    10081 tagcgatatg accccggaac aattagatct ggaagttaag aaaattaccg cctatgccaa
    10141 acttgcggca ggtacagttg caggcgtaac gggaggagat gtcaatactg ctgcacaaac
    10201 cgcacaaaac gcggtagaaa ataatgcggt taaagctgtt gtaactgctg caaaagtggt
    10261 ttataaggta gccagaaaag gattaaaaaa cgggaaaatc aacgttagag atttaaaaca
    10321 gacgttgaaa gacgaaggtt ataatttagc cgacaacctg accaccttat tcgacgaaac
    10381 attggattgg aacgatgcca aagccgttat tgatattgtc gtcggaacag agctgaatcg
    10441 cgctaataaa ggggaagcgg cacaaaaggt caaggaagtt ttagaaaaaa atcgtcctta
    10501 tatccctaat aaaggtgctg taccgaatat gagtacatac atgaaaaata atccttttgg
    10561 aaaacagctg gctcaaattt cagaaaagac aacgcttccg acgcagcaag ggcagtctgt
    10621 cttcttggta aaaagaaacc aagggttatt aaaaaccggt gataggtttt atttagatgg
    10681 ccaacataaa aatcatttag aggtttttga taaaaatggg aactttaagt ttgttctaaa
    10741 tatggatggt tcgcttaacc aaatgaaaac tggggcagca aaaggtcgta aattaaactt
    10801 aaaataggaa attttatgga aacattgaat gatataaaaa aaatcttgat taatgtgggg
    10861 ctttatcaag ggtttgattt gacagatcca aaagtatcag aagaagttaa tcatgaaaca
    10921 gctaatatga aatggattaa agattatact tcagacggga attgggataa tgaatttaag
    10981 gaggatttaa aaaacttttt agattatatg gaagtatgcc aattagccct aaacgataaa
    11041 aatttcaaaa ttgccagtaa ttctttattt atggctatga tttacgcagg taatctatct
    11101 cttatatttg attcaataaa aactgatata tcaacattat tgagtgctga gtataaaaag
    11161 aatagttttt catggccatc tcttgatgaa tagaaagcaa gttgtagcct gcatgaaatc
    11221 taaaacccat gcataaggtg tgggcttcag tatacgcgtt ccatgattta cggccatatg
    11281 ccgtctgaaa agctcaattt tttcagacgg catttgttat gaaagtaaat atttagattc
    11341 cctgtatact gtttagactc gtgtgtgctg agtaagctgt agtctgcatg aaacctaaaa
    11401 ctcgctcaaa attaagctaa gacattagca gggcaagggc gaaaattgaa tcttaaataa
    11461 ggtgattcag atgaaaaatt ttaatgtagt aaaagaaagt ttaagagagt taggaattaa
    11521 acaaggattt gatctttatg agaaagccac aactgaaaaa ttgaatagtg aagatcctct
    11581 tgacttacaa tggctttcta actattcatc tgattggaat gatgaattag aagaagactt
    11641 tgattctttt tttcagcata tgaaggaata tcaatatgct attgacaatg aagacattaa
    11701 atctgcatgt agttcactat gtgaagctat gctctatgtt ggtaatatta aaaatttttt
    11761 tgagtttctc aaaagcgata tgattagact gttgagaggt gaaagtaaaa caacagactt
    11821 tcaatggccg caatttgatg aatagcagca agctgtagcc tgcatgaaac ctaaaatcca
    11881 tgcgtaaggt gtgtgcttca gcacgcacgc gttccatgat ttacggctca atgccgtctg
    11941 aaaagctcac aatttttcag acggcatttg ttatgcaagt aaatattcag attccctata
    12001 tactgcccag atgcgtgcgt gctgaagaca ccccctacgc ttgctatttg aaacagctcc
    12061 aagtcaccaa agacgtcaac tggaaccagg tacaactggc gtacgacaaa tgggactata
    12121 aacaggaagg cttaaccgga gccggagcag cgattattgc gctggctgtt accgtggtta
    12181 ctgcgggcgc gggagccgga gccgcactgg gcttaaacgg cgcggccgca gcggcaaccg
    12241 atgccgcatt cgcctcgctg gccagccagg cttccgtatc gctcatcaac aacaaaggca
    12301 atatcggtaa caccctgaaa gagctgggca gaagcagcac ggtgaaaaat ctgatggttg
    12361 ccgtcgctac c
//
LOCUS       DAWIVB010000040        12231 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWIVB010000040 DAWIVB010000000
VERSION     DAWIVB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.040"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            243..980
                     /locus_tag="WIX60_000824"
     CDS             243..980
                     /locus_tag="WIX60_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ5305114.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAD
                     NVAFPLEIAGWPSEKIKARVKECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSDIGRELIRED"
     gene            982..1668
                     /locus_tag="WIX60_000825"
     CDS             982..1668
                     /locus_tag="WIX60_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ5305115.1"
                     /translation="MADLTFQQAVSTIVGMKDEIFRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKLVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIIFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1827..2690
                     /locus_tag="WIX60_000826"
     CDS             1827..2690
                     /locus_tag="WIX60_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305116.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2752..3321)
                     /locus_tag="WIX60_000827"
     CDS             complement(2752..3321)
                     /locus_tag="WIX60_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ5305117.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3479..4339
                     /locus_tag="WIX60_000828"
     CDS             3479..4339
                     /locus_tag="WIX60_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ5305118.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEVKTANHKKGRIVLYFDTPETFGHL
                     LEQLGIDYRP"
     gene            complement(4535..4972)
                     /locus_tag="WIX60_000829"
     CDS             complement(4535..4972)
                     /locus_tag="WIX60_000829"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305119.1"
                     /translation="MFLRDLFALRGNNKKSTRNCSSLSVGLSAVWGICSINKRSSENI
                     FRRPFPNKGLATACRFKQSIALTFAFVRSASQTNCIGSGNADCADYTGNIALAHALTH
                     SCGKVDAYAFIVAVADFYGIWADAQGKRGTFLKFLVGNGYIID"
     gene            5181..5534
                     /locus_tag="WIX60_000830"
     CDS             5181..5534
                     /locus_tag="WIX60_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ5305120.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5524..6390
                     /gene="atpB"
                     /locus_tag="WIX60_000831"
     CDS             5524..6390
                     /gene="atpB"
                     /locus_tag="WIX60_000831"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ5305121.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6447..6683
                     /gene="atpE"
                     /locus_tag="WIX60_000832"
     CDS             6447..6683
                     /gene="atpE"
                     /locus_tag="WIX60_000832"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ5305122.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6754..7224
                     /locus_tag="WIX60_000833"
     CDS             6754..7224
                     /locus_tag="WIX60_000833"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ5305123.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7229..7762
                     /locus_tag="WIX60_000834"
     CDS             7229..7762
                     /locus_tag="WIX60_000834"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ5305124.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELVQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7773..9320
                     /locus_tag="WIX60_000835"
     CDS             7773..9320
                     /locus_tag="WIX60_000835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ5305125.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTCGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9345..10220
                     /gene="atpG"
                     /locus_tag="WIX60_000836"
     CDS             9345..10220
                     /gene="atpG"
                     /locus_tag="WIX60_000836"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ5305126.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCFGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10258..11655
                     /gene="atpD"
                     /locus_tag="WIX60_000837"
     CDS             10258..11655
                     /gene="atpD"
                     /locus_tag="WIX60_000837"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ5305127.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRAIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11666..12088
                     /locus_tag="WIX60_000838"
     CDS             11666..12088
                     /locus_tag="WIX60_000838"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ5305128.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKVLAAAIAQLKTLDYIRSHKK"
BASE COUNT         2997 a         3129 c         3160 g         2945 t
ORIGIN      
        1 tcaattattt gccgttttgg tgcgaaccgc tgcctttgcc cgtttcagac ggcattgtcc
       61 gaaatggttg cccgcttcct gctttattga caaaaaaatg ctttcccgat aatatcctac
      121 gaaaattaac ctgccgattt gacacagctt gcgggcataa cagctaaagc gttccgacaa
      181 tttcagcttt atcttccgcg cccgttgtgt ccgacatcgg gctttgttgt atgggaaaga
      241 caatgattat tttggacaag gtttccaagc attaccaaac gcgcgacaag acccgttttg
      301 ccgccgtcga gccgaccagc ctcgaaatcc gcgacggcga aatcttcggg ctgatgggtt
      361 attcgggtgc aggcaaatcc accctgttgc gcctgattaa cctgttggaa cgccccgaca
      421 gcggcaaggt caacgtctgc ggacaagagc tgaccgcgct cgatgccgcc gcattgcgtc
      481 aggctcggca gaatatcggc atggtgtttc agcagtttaa tcttttgagc aaccgcaccg
      541 ttgccgacaa tgttgccttt cctttggaaa tcgccggatg gccgtctgaa aaaatcaaag
      601 cgcgcgttaa agaatgcctt gaaatcgtcg gcttgaccga acgcgccggc cactatcccg
      661 cccagctttc cggcgggcag aaacaacgtg tcggcatcgc ccgcgcactc gcgcccaaac
      721 cccaagtcat cctcgcagac gaacccactt ccgccctcga ccccgccacc acgcgcagcg
      781 tcttggaatg tttggaagac atcaacaaac gcttcaacgt aaccatcgtc atcgtaaccc
      841 acgaaatgag cgtcatccgc cgcctgtgcg accgcgccgc cctcttggat aaaggcaaag
      901 tcgtcgaaat cgtcgaagta cgcggcaacc aaatccacgc ccaatccgac atcgggcgcg
      961 aactgattcg ggaggactga tatggcagac ttaacattcc aacaagccgt ttccaccatc
     1021 gtcggcatga aagacgaaat cttccgcgcc ttgggcgaaa ccttcgtgat ggtcggcttg
     1081 tccaccacat tcgccgtcat cttcggcacg ctgctgggcg tgctgctctt cgtaacctcc
     1141 agccgccaac tgcattacaa caagctggtg aacttcctgc tcgacaacct cgtcaacctc
     1201 atgcgcgcct tccccttcgt catcctgatg attgcgatga tacccgccac acgcgccatc
     1261 gtcggcagca ccatcggtcc ggttgccgcc tcgctggtgt tgagcgtgtc gggattgttt
     1321 tattttgccc gactggtgga acaaaacctg cgcgaagtcc ccaaaggcgt aattgaagcc
     1381 gccgccgcga tgggtgcgcc gccgattgcc atcgtctgca aagtcctctt gaacgaagcg
     1441 cgcgcgggca tggtttccag cattaccgtg cttgccatcg ggcttttgtc atacagcgcg
     1501 gcggcaggga tgataggcgg cggcggcttg ggcgacctcg ccatccgcta cggctactac
     1561 cgctaccaaa ccgaagtcat catcttcatc gtcgccctcc tcgtgctgct ggtcatcctg
     1621 attcaaagca ccggcaacgc gttggcgcgg aaactcgaca aacgttgaac ccgaatgccg
     1681 tctgaacgcc aaaaccccca ccgctatccg aaaaatgcta taaaatcccc ctgttcgcgg
     1741 caaatgccgt ctgaacgccg aatccggacg gcaggactcc ctgcccgtca tttttgtttg
     1801 aaactgccac aacatcagga gaaaatatga aaaccttctt caaaaccctt tccgccgccg
     1861 cactcgcgct catcctcgcc gcctgcggcg gtcaaaaaga cagcgcgccc gccgcatccg
     1921 cttctgccgc cgccgacaac ggcgcggcga aaaaagaaat cgtcttcggt acgaccgtcg
     1981 gcgacttcgg cgatatggtc aaagaacaaa tccaagccga gctggagaaa aaaggctaca
     2041 ccgtcaaact ggtcgagttt accgactatg tacgcccgaa tctggcattg gctgagggcg
     2101 agttggacat caacgtcttc caacacaaac cctatcttga cgacttcaaa aaagaacaca
     2161 atctggacat caccgaagtc ttccaagtgc cgaccgcgcc tttgggactg tacccgggca
     2221 agctgaaatc gctggaagaa gtcaaagacg gcagcaccgt atccgcgccc aacgacccgt
     2281 ccaacttcgc ccgcgtcttg gtgatgctcg acgaactggg ttggatcaaa ctcaaagacg
     2341 gcatcaatcc gttgaccgca tccaaagcgg acatcgccga gaacctgaaa aacatcaaaa
     2401 tcgtcgagct tgaagccgcg caactgccgc gtagccgcgc cgacgtggat tttgccgtcg
     2461 tcaacggcaa ctacgccata agcagcggca tgaagctgac cgaagccctg ttccaagaac
     2521 cgagctttgc ctatgtcaac tggtctgccg tcaaaaccgc cgacaaagac agccaatggc
     2581 ttaaagacgt aaccgaggcc tataactccg acgcgttcaa agcctacgcg cacaaacgct
     2641 tcgagggcta caaatcccct gccgcatgga atgaaggcgc agccaaataa ggcagtcgta
     2701 taaaatgatg ccgtctgaac tgtatccgtg ttcagacggc atttttgtcc tttaatccgc
     2761 cattccctgc cattccgccg aatccggcgt atcgattccg aacagcgaca aagcgtgtgc
     2821 aacactgtgc gccactatgt cgtccgccgt ctgcggtttg cggtacatcg caggaacagg
     2881 gggaaacacc acgccgccca tttccgttac ccgcttcata ttgtccaaat gggcaaggtt
     2941 cagcggcgtt tcgcgcacca tcagcaccag ccgccgcctt tccttcaaaa ccacatccgc
     3001 cgcacgcgtc agcagattgt cgccgaagcc gtgcgcgaca gaggcaagcg tccgcatcga
     3061 acagggggcg accagcatcc catccgtttt aaacgtaccg ctggcaatgc acgccccgat
     3121 attgccgatc ggatgcacga agtccgccaa ggcatatacc tcgtctctcg cataagccgt
     3181 ttccgaagcg cgcgccatct ccgcaccttt cgataccaca aggtgcgttt cgacatcttg
     3241 cgcgcgcaaa agttccaaag ccttcacgcc gtattggaaa ccgctcgccc cgctgatgcc
     3301 gattatcaaa cgccgtacca tcatccgcct ttcccataaa accgcctgca acggcaaacc
     3361 ggctattata gtgaaaaaac agaaatccga taaacgcgga tacaaattgt cggcaacacc
     3421 caatatccga taaaataccc gatttaacat cctatctgaa taggcacggg agggcggtat
     3481 ggcaaaagta aaaggcggat tggggcgcgg cttggattcg ctgctcgcca acggcgcgga
     3541 caacagcagc ggcgaccgat tgaccacggt tgcggttaaa gatatccggc ccggccgcta
     3601 tcaggcgcgt gttcaaatcg atgacgaagc cttgcaggaa ctggcagatt cgattaaggc
     3661 gcaaggcgtg atacagcccg tcatcgtgcg cgaacacgga ctgtcccgat acgaactgat
     3721 tgcaggcgaa cgccgttggc gcgccgcaca gattgccggc ctgaccgaaa tccccgccgt
     3781 tatcaaaacc atcagcgacg aaaccgcatt ggcaatgggt ttgatcgaaa acctccagcg
     3841 cgaaaacctc aaccccatcg aagaagcaca aggcttgaaa cgccttgccg acgagttcgg
     3901 gctgacccac gaaaccatcg cccaagccgt cggtaaaagc cgaagcgcga tttccaacag
     3961 cctgcgcctt ttaagcctgc ccgaacccgt gcaggaaatg ctttaccaac gccgcctcga
     4021 aatggggcac gcccgcgcat tgctgaccct gcccgtcgtc gaacagctcg aattggcgca
     4081 aaaggccgtc aaaaacggct ggtcggtgcg cgaagtcgaa cgccgcagcc aggccgccct
     4141 tcaaaacaaa cgtcccgagc ccaaaaagac tgccgccgcc gacatcggcc gcctgaatga
     4201 tttgctgact gaaaaactgg gtgtcaacgc tgaagtcaaa accgccaacc acaaaaaagg
     4261 caggattgtc ctgtatttcg atacgcctga aacgttcggc cacctgctgg agcagttggg
     4321 catagattac cggccttaat tttgcgggat ataccgtctg aaatatagag aatagctttc
     4381 cagattttaa gtgggaaata taattctatt gacatttttt ctgcttcacg taagaatcgt
     4441 tttcctgttt tcatttttaa ttttcgagga aattatgaac acacgcatca tcgtttcggc
     4501 tgcgttcgtt gcgttggcat tagcaggttg cggctcaatc aataatgtaa ccgtttccga
     4561 ctagaaactt caggaacgtg ccgcgtttgc cttgggcgtc agcccaaatg ccgtaaaaat
     4621 cagcaaccgc aacaatgaag gcatacgcat caactttacc gcaactgtgg gtaagcgcgt
     4681 gagccaatgc tatgttacca gtgtaatcag cacaatcggc gttaccactt ccgatgcaat
     4741 ttgtttggga ggcggaacgc acaaaggcaa aagtcaatgc aatgctttgc ttaaagcggc
     4801 aggcagttgc taatccttta ttcggaaaag gtcgtctgaa aatattttca gacgaccttt
     4861 tatttattga gcaaattccc caaactgccg acaatccgac cgataaactg ctacaatttc
     4921 gcgttgattt tttattattt ccacgtaaag caaacaaatc acgtaagaac attaaaaatg
     4981 taaagaaatt gcggacaaac tccttaatat cctgtaatta tatgtaatgt gtgttagaat
     5041 gacacctgtc gccgttattc aggcggcgag cgcggcggat tgggatacgt tgtttcacaa
     5101 agattaactg tcgcttgacg taataaggct tctaaattat attagccgcg ccccaaatcc
     5161 gaggcggaaa cattcccttt atgaagcaga ttatcatcct acaatccgcc gtattgtcca
     5221 tatgcgccgc agttgccttt gctgtatggg gttttgccgg attcctttcg gcggtcggtg
     5281 gcggtttgtc ctacctgctt cccacgtttg ttgcagtttt acttttaaaa cttttcaggg
     5341 gaaacccctt cctgcaaagc aggatgttcg tcttcggaga gattttaaaa gtagtgctgt
     5401 cgctgttgtc catgcttgcc gtatttgcaa tatggcatca atcgctggtg ttcgccccgt
     5461 ttctgatggg gctgctcggt gtcagccatt tggttttttt agtattgttg agagtgaaag
     5521 attatggcag gtgaaaccat taccgctgcc gactacatca agcaccactt gcagagcttg
     5581 accagtttgt cggatgttac tcagggtcag ggactgaaaa acattgctga tttttcgttt
     5641 attaaccttg atgccgtctt ttttgccgtc ctgttgggcg taatcggcag ctttctgttg
     5701 tggcgcggtg caaaaaaagc gacggcaggc gttcccggac gtttccaggc cgcagtggaa
     5761 atcttgtttg agtttgtgga cgatatgtgt aagagcatca ttcacaacga aaagtctaga
     5821 aaagccgtcg cgccgttggg tctgacgctg tttgtctgga tttttctgat gaacgcgatg
     5881 gatatgctgc cggtcgattt gctgccgatg gtatggcagg gcattaccgg caaccatcac
     5941 gccctgctgc gcgtcgtacc gactgccgat ttgaacacca ctttggcact cgctgtcggc
     6001 gtgttgctga tttgtatcta ttacaacatc aaaatcaaag gattgggtgg ctggtttcac
     6061 gagttgttca gcgcgccgtt cggtgcaaaa ctcgcacctg cgaactttct gttgaatttg
     6121 gtcgagtttc tttctaaaac cgtatcccac ggtatgcggt tgttcggcaa tatgtatgcg
     6181 ggcgaattgg tattcttgtt gatagccttg cttggcggtg cttgggcggc ttccggcagc
     6241 gtcgaagtca tggatccgat tctgtttgta ttccacatta ttgccggttt ggcatgggcg
     6301 attttccata ttttggtgat taccctgcag gcgtttattt tcatggcgtt ggcgttcgtc
     6361 tatatcggac aggcacatga tgcgcactga ttttcctaag tagtttgttt ttgtagttta
     6421 acctttgttt ctttaaggag tttaaaatgg gtttgattgc tatcgcatgt ggtttgatcg
     6481 ttgcattggg tgcattgggt gcctctatcg gtatcgcaat ggtcggttct aaatatttgg
     6541 agtcttctgc ccgtcagccc gaactgatcg gtccgctgca aaccaaactg ttcctgattg
     6601 ccggtttgat tgatgccgca ttcttgattg gtgtcgccat tgcactgctg ttcgccttcg
     6661 ttaacccgtt tgcaggtgca taatcaggac ggattcgtcc gttccgtaca ggcgcagcct
     6721 gtttgattaa ccccagtacg aaggttaagt aacgtgaata tcaatgcaac attattcgct
     6781 caaatcatcg tctttttcgg tttggtatgg tttaccatga aattcgtgtg gccgccgatt
     6841 gcaaaagctt tggatgagcg tgccgcaaaa gtcgccgaag gcttggctgc cgccgagcgt
     6901 ggtaaaagcg attttgagca ggctgaaaaa aaggttgcag aactcttggc agaagggcgt
     6961 aatcaggttt ccgaaatggt tgccaacgcc gaaaaacgtg ccgccaaaat tgtcgaagaa
     7021 gccaaagaac aggcttcttc cgaggcggcg cgcattgcag ctcaggcaaa ggccgatgtg
     7081 gagcaggaat tgttccgcgc acgcgaatcc ctgcgcgagc aggttgccgt gttggctgtc
     7141 aaaggtgccg aatctatttt gcgcagcgaa gtcgatgctt ccaaacacgc aaaactgctc
     7201 gataccctga aacaggagct gtaatcttat ggcagagttc gcaacgattg ccagacctta
     7261 tgcaaaggca ttgttcggtc tggctcagga aaaaaaccaa attgagtctt ggttgggcgg
     7321 actggaaaaa cttgcggcgg ttgttcagga agggaaggtg gcttcattga ttgaccgtcc
     7381 tgaaacgaat gcttcagaaa aagcagatat cctcatcgat ttggtcggtt tgaaagacaa
     7441 ggagttgaaa aactttgtta tcgtcttggc cgggcagaaa cgtttgtcga tattgccgga
     7501 agtatatgct caatatcaag acttgacctt atcattcaac catatcaaat ctgccgtcat
     7561 ttacagtgcc tatccgttga ccgacaaaca ggtcggcgag ttggtgcaaa tgctgaataa
     7621 gcgtttcgac agcgagctga aaatctctgt cgaaatcgaa ccggagctga ttggcggcat
     7681 aaaagttgaa gtgggtgatc aggttttgga tttgtctgta caaggcaaat tgagtgcctt
     7741 gtacacgact atgacgaatt aggagagttt tcatgcagct taatcctgct gaaattagcg
     7801 atttgattaa agccaagatc gaaaatctgt ctgtaaatgc cgaagtgcgt acctgtggta
     7861 cggtaatttc ggtaactgac ggtatcgttc gcatccatgg tttgtcagat gcaatgcaag
     7921 gtgagatgct cgaattcccg ggtaacactt tcggcttggc gatgaacctg gagcgcgact
     7981 ccgtcggcgc cgtagtgttg ggcgagtacg aacacattaa agaaggcgac acggttacct
     8041 gtaccggtcg tatcttggaa gtgccggtcg gacgcgaact ggtcggacgc gtcgttgatg
     8101 cattgggtcg gcctatcgac ggcaaaggcc cgattaatac aactttgacc gcccctatcg
     8161 aaaaaatcgc accgggcgtg attgcgcgca aatcggttga ccagccgatg caaaccggtc
     8221 tgaaggcgat tgactctatg gttcctgtcg gtcgcggtca gcgtgagttg attattggcg
     8281 accgtcagac aggtaaaacg gccgtagcat tggatgccat cgtcaaccaa aaaggtacgg
     8341 gtgttatctg tatctatgtc gctatcggtc aaaaagcatc ttctattgcc aacgtggtcc
     8401 gcaaattgga agagcatggc gcgatggagc ataccatcgt ggttgctgca actgcatctg
     8461 aagcggcggc attgcaatat atcgcacctt actccggttg tacgatgggt gaattcttcc
     8521 gcgatcgtgg cgaagatgcc ttgattgttt atgacgattt gtccaaacag gctgtggctt
     8581 accgtcaaat ttccctgctt ttgcgccgtc cgcccggccg cgaagcctat cccggcgatg
     8641 tgttctacct gcactcccgt ctgttggaac gtgcggcacg tgtcaatgaa cacgaagtgg
     8701 agaaattgac caacggcgaa gtaaaaggca aaaccggttc tctgaccgcg ttgccgatta
     8761 tcgaaaccca agcgggcgac gtatctgctt tcgttccgac taacgtcatt tcgattaccg
     8821 acggtcagat tttcttggaa accgacctct tcaacgccgg tatccgtcct gcaatcaatg
     8881 ccggtatttc cgtatcccgc gtaggcggtg ctgcacaaac caaagtgatt aaaaagctgg
     8941 gtggcggtat ccgtttggcg ttggcacaat atcgtgaatt ggcggcgttc tcgcaatttg
     9001 catccgattt ggatgaagct acgcgcaaac aattggagca tggcgaagtc gtaaccgaac
     9061 tgatgaaaca gaaacagttc agtacgttga atacggctga aatggctttg accctttggg
     9121 caatcaacaa cggttcgtat tctgatgttc cggttgccaa agctctggct tttgaatctg
     9181 aatttttgag ctttgtccgt acccaacatc cggaagtttt ggaagccgtc aatgcttcag
     9241 gtgcaatgtc cgacgagagc gagaaaacgc ttgaagcagc catgaaatcc ttcaaatctt
     9301 cttacgccta tcaggcataa ggcttgaagt gaaaggagtc tgaaatggca gtaggaaaag
     9361 agattctcac caaaatccgc agtgttcaga atacccaaaa gatcactaaa gcgatgcaga
     9421 tggtgtcaac ctctaaaatg cggaagactc aggaacggat gcgtttggcg cgtccgtatg
     9481 ccgaaaaagt gcgtatggtg atgagccatc ttgcgcaaac caataccgat catggtattc
     9541 cgttactgga atctcatcgg gaaatcagac gtgtcggttt tattttgatt acgtctgata
     9601 agggtttgtg tggcggtttg aacgccaacg tgctgaaaaa gtttttggca caagttcaag
     9661 agtatcggaa tcaaggtatt gaagaggaaa tcgtatgctt tggcagtaaa ggtctgatgg
     9721 cgtgtcagag cattggtctg aatgtggttg ccagtgccgt aaatttgggc gataccccaa
     9781 aaatggaaat gctgctcgga cctttgacag aactcttcca acggtatgag aaacatgaaa
     9841 ttgacagaat ccatctggtg tattcgggtt ttgtcaatac catgcgtcaa gaaccgagaa
     9901 tggaagtatt gctgcctatc ggtgagaacg tgattggcga ttcagctccc aaatcaccgt
     9961 tcagctggga ataccgctac gaaccgactg cacttgcagt gttggaatat ctggttcgcc
    10021 gctatttaga gtctgtggtt tatcaggcgt tgagcgacaa tatggcatcc gaacaggcag
    10081 cccgcatggt tgccatgaaa gctgcaacag acaatgcagg caatgccatc aaagagttgc
    10141 gtttggtata taacaaatcg cgtcaagctg cgattaccac ggaattgtca gaaattgtag
    10201 caggtgcggc ggctgtctga tgccgtctga atcctgtacg gaaattagga tacgataatg
    10261 agccaaggca aaatcgtaca aattatcggt gcggttgttg acgtggaatt tccacgcgac
    10321 atgattccgc gcgtttacga cgctttgaaa ttagacgaaa acggtctgac tttggaagtc
    10381 caacagcttt tgggcgacgg cgtagtccgt gccattgcga tgggcagctc ggacggtttg
    10441 aaacgcggca tgactgtgag caataccggt gcgcccatta ctgtgccggt aggtaaaggt
    10501 acgttgggac gcattgtcga tgtattggga acgcctgttg acgaagcagg tccgatcgat
    10561 accgacaaga gccgtgccat ccaccaagct gcccctaagt ttgacgaact gtcttccaca
    10621 accgaattgc tcgaaacggg cattaaagtg attgacttgc tgtgtccgtt tgccaaaggc
    10681 ggtaaagtag gtctgttcgg cggtgccggt gtgggtaaaa ccgtgaacat gatggaattg
    10741 atcaacaaca tcgccaaagc gcacagcggc ttgtccgtgt tcgcaggcgt gggtgagcgt
    10801 acccgcgaag gtaacgactt ctaccacgag atgaaagatt ccaacgtatt ggataaagtg
    10861 gcaatggttt acggtcagat gaacgaacct ccgggcaacc gtttgcgcgt cgcattgacc
    10921 ggtttgacca tggcggaata cttccgtgac gaaaaagacg aaaacggcaa aggccgcgac
    10981 gtattgttct tcgtggacaa catctaccgt tacaccctgg ctggtaccga agtatccgca
    11041 ttgttgggtc gtatgccgtc tgcagtgggc taccaaccga cattggcaga agaaatgggt
    11101 cgtttgcagg agcgtattac ctctacccaa accggttcta ttacttccat ccaagccgta
    11161 tatgtacctg cggacgactt gaccgacccg tctccggcaa caacgttcgc ccacttggac
    11221 gcaaccgtcg tattgagccg cgatattgcc tctttgggta tttacccggc agtcgatccg
    11281 cttgattcta cttcgcgcca actggatccg atggtattgg gtcaagagca ctacgacgtg
    11341 gcgcgcggtg tgcagtccac cctgcaaaaa tacaaagaat tgcgcgacat catcgccatc
    11401 ttgggtatgg acgaattgtc tgatgaagac aaactgaccg taatgcgtgc gcgtaaaatc
    11461 caacgcttcc tgtcccaacc gttccacgtt gccgaagtgt ttacaggttc tccgggtaaa
    11521 tatgtcgccc tgcgcgatac cattgccggc ttcaaagcca tcttgaacgg cgaatacgat
    11581 catctgcccg agcaggcatt ctatatggtc ggcagcattg aagaagcagt agagaaagcg
    11641 aaaaccttaa actaaggagg tcggcatgag catcatgcaa gttgaggtgg taagtagcga
    11701 gcagaaaatc tattcaggcg aggcaacatt tatcgttgtt ccgactgtac agggcgaact
    11761 cggtatttat ccgcgacacg agccgattat gagtttggtg cggccggggg ctttgcgttt
    11821 gaccgttccg ggcgaggata aagaggtttt ggttgctgtt tccggcggtg ttttggaagt
    11881 acagcctgat aaagtaactg tcttggcgga tgttgccgtc cgcagtgcgg agatggatcg
    11941 ggcacgtgcg gaagaggcga aaaaagccgc agaagcgggc atttcccaag ctaaagacga
    12001 taaggctttg gcggaagcgc ataaagtatt ggctgctgcg attgcacagc tcaaaacttt
    12061 ggactatatc cgttcgcaca agaaataaac ttgtttggtt tggaaaagca cgaccgtttg
    12121 gtcgtgcttt ttattttccg gatttaaatt aaagactgtg tttggatatg gcgttggcag
    12181 gcatagcctg ctttaaggta ttcaataggg cagcctgccg cctaaccgaa a
//
LOCUS       DAWIVB010000041        11797 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWIVB010000041 DAWIVB010000000
VERSION     DAWIVB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.041"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..801
                     /locus_tag="WIX60_000839"
     CDS             <1..801
                     /locus_tag="WIX60_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ5305129.1"
                     /translation="RTPNIKYIYQDNQGLSEARNTGIKNSNGKYIVFIDSDDFINCQI
                     LLDFLSKDDTDMPDVVFLNAVKYDKGSVSYFGEDYQPEKILNQSKVEVLKGLCRFRKF
                     PGSAWNKIIKRELIIREKLFFERGIYSEDIEWSMRLFNAATTFSYLDGCYYYYRQGRK
                     DSITGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIECD
                     ADIKRRAYHLRFMLLKSNKLIYKLIFPIITLLGVDITGRILKAIRGNI"
     gene            842..1372
                     /locus_tag="WIX60_000840"
     CDS             842..1372
                     /locus_tag="WIX60_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ5305130.1"
                     /translation="MNAIRTFQNRTPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1406..1522)
                     /locus_tag="WIX60_000841"
     CDS             complement(1406..1522)
                     /locus_tag="WIX60_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ5305131.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(1541..1728)
                     /locus_tag="WIX60_000842"
                     /pseudo
     CDS             complement(1541..1728)
                     /locus_tag="WIX60_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(1747..3342)
                     /locus_tag="WIX60_000843"
     CDS             complement(1747..3342)
                     /locus_tag="WIX60_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ5305132.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LKIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3449..3844)
                     /gene="hisI"
                     /locus_tag="WIX60_000844"
     CDS             complement(3449..3844)
                     /gene="hisI"
                     /locus_tag="WIX60_000844"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ5305133.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRALRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3875..4642)
                     /gene="hisF"
                     /locus_tag="WIX60_000845"
     CDS             complement(3875..4642)
                     /gene="hisF"
                     /locus_tag="WIX60_000845"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ5305134.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAANPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4655..5392)
                     /gene="hisA"
                     /locus_tag="WIX60_000846"
     CDS             complement(4655..5392)
                     /gene="hisA"
                     /locus_tag="WIX60_000846"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ5305135.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQTVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5425..6063)
                     /gene="hisH"
                     /locus_tag="WIX60_000847"
     CDS             complement(5425..6063)
                     /gene="hisH"
                     /locus_tag="WIX60_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ5305136.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALKRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(6194..7696)
                     /gene="pta"
                     /locus_tag="WIX60_000848"
     CDS             complement(6194..7696)
                     /gene="pta"
                     /locus_tag="WIX60_000848"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ5305137.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRNANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7881..8939)
                     /gene="fbpC"
                     /locus_tag="WIX60_000849"
     CDS             complement(7881..8939)
                     /gene="fbpC"
                     /locus_tag="WIX60_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ5305138.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8960..10477)
                     /gene="fbpB"
                     /locus_tag="WIX60_000850"
     CDS             complement(8960..10477)
                     /gene="fbpB"
                     /locus_tag="WIX60_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ5305139.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10545..11540)
                     /gene="fbpA"
                     /locus_tag="WIX60_000851"
     CDS             complement(10545..11540)
                     /gene="fbpA"
                     /locus_tag="WIX60_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ5305140.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
BASE COUNT         2517 a         2787 c         3585 g         2908 t
ORIGIN      
        1 cggacaccaa atatcaaata tatatatcag gataaccaag ggttgtcgga ggcgagaaat
       61 accggaataa aaaattcaaa cggaaaatat atagtcttta ttgattcgga tgattttatt
      121 aactgtcaga ttttgctgga ttttcttagt aaagatgata ctgatatgcc ggatgtggtg
      181 tttttaaatg cggttaaata tgataaggga agtgtttcat attttggcga agattatcag
      241 cctgaaaaaa tactcaatca atccaaagtc gaagttttga aaggattatg ccgatttaga
      301 aaatttccgg gttcggcgtg gaataagatt ataaaaagag aattgattat tagagaaaaa
      361 ctgttttttg aaaggggaat ttattctgaa gatatcgaat ggtcaatgag gttatttaat
      421 gcggcaacaa ctttttctta tttggacggt tgttattact attatcggca gggaagaaaa
      481 gattctatta cgggaactgt ttcggaaaaa agtataaagt cattattata tattttggag
      541 aaaaatgcgg aaatggaatt taatagggat atatcgagtt atctttattc ttttctttcc
      601 tacgaatatc tcgttttgct ttttataatg acgagtaaaa atatagagtg tgatgctgat
      661 ataaaaagaa gggcgtatca tttaaggttt atgctgttaa agtccaataa attgatatat
      721 aagctgatat tcccgataat cacattactc ggggtcgata ttacaggcag gattttaaaa
      781 gcaatcaggg ggaatattta ataaatcctt taacaatata taccttaccg aaggaggaaa
      841 aatgaacgca atccgaactt tccaaaaccg cacgcccgaa atccacgaaa cctgtatgat
      901 agacgaagcc tgcgtcgtca ttggcgaagt gtcgcttgcc gaagatgttt ccgtgtggcc
      961 gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca gcaatataca
     1021 ggacggcagc gtcttgcacg tttcccacaa aaccgccgcc aaacccgaag gatcgccgct
     1081 ggttatcggc gaagacgtta ccgtggggca caaagtgatg ctgcacggct gccgtatcgg
     1141 caaccgcgtc ctggtcggca tggggacgac ggttctggac gatgccgtga ttgaggacga
     1201 agtgatgatc ggcgcgggca gcctcgttcc gccgcgcaaa cgcttggcgg gcggctatct
     1261 ttatgtcggt tcgccggtca gacaggtgcg cgtgctgacc gatgaggaaa aagccttttt
     1321 gaaatattcc gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt gaaatcacat
     1381 cggcgttctt gcgtcagccc caaattcatg cggatgggac gcatccgata acggtatccg
     1441 atgcgccttg attttgaccg tctgcgtttg aattgcaggc aaaaatgccg tctgaaagcc
     1501 ttttttcggg ttcagacggc attttattgc cgattgtttt ttaaagtttg accgaatgtt
     1561 cgcgcgtttc gtggaacacg atgtccggcc agcgttcttg cgtcagccct aaattcatga
     1621 ggacgggatg cccgataacg gtatccgatg cgtcttgatt ttgatcggtg catttgagtt
     1681 gcaggcaaaa atgccgtctg aaagcctttt ttcgggttca gacggcattt tatcgccgat
     1741 tgctttttac agtttgaccg aatgttcgcg tgtttcgtgg aacacgatgt ccggccaacg
     1801 ttcttgcgtg agtcccaaat tcacgcggtt gggggcgagg taggcgaggt tgccgcctgc
     1861 gtcgatggcg aggttgcccg cgttggcttt ttcaaattca gccagttttt tcttgtcgtc
     1921 gcacgatacc cagcgcgccg accagatgga tgcgctgtcg aacacggctt ctacgccgta
     1981 ttcgttggcg aggcgcgagg taacgacttc aaactgcaac acgccgaccg cgcccaaaat
     2041 caaatccgcg ccgctcatcg gtttgaacac ctgcaccgcg ccttcttcgc cgagctgttg
     2101 caagcctttt tgcagttgtt tgattttcag cgggtttttg atgcgtacgc tgcggaacag
     2161 ttcgggtgcg aagaatggga tgccggtgaa cgccagttgt tcgccttcgg agaagctgtc
     2221 gccgatttgg atgttgccgt ggttcgggat gccgataatg tcgccggcgt aggcttcttc
     2281 aaccagctcg cggtcgtgcg acatgaaggt aaccacgctg gaggcggcga tttcgcggtt
     2341 gatacgcagg tgtttcatct tcatgccgcg ctcgaatttg ccggagcaga cgcgcaagaa
     2401 ggcaatacgg tcgcggtgtt tcgggtccat attggcttgg attttgaaga taaatccgga
     2461 aaacttcggc tcgtccggct cgaccatacg tacggtcgcg tcgcgcggtt tcggcgcggg
     2521 cgcccagtca atcaatgaat tgaggatttc ctgaataccg aagttgttaa tcgcagagcc
     2581 gaagaatacg ggcgtgagtt cgccggcgag gaattcgtcg agattaaact cgttggaagc
     2641 cgcctgcacc aattcgattt cgtcgcgcaa ctgctggatt tccaacggaa agcgttgttc
     2701 caattcagga ttatcgatgc ctttgatgat gtcgaactcg tgcggcaggc gttcgccgcc
     2761 agcttcaaag agataaattt catcgttcag gatgtggtac acgcccttga agtttttgcc
     2821 cataccgatc ggccaggtaa cgggcgcgca gcggattttt aaaatgtttt ccacttcgtc
     2881 caaaagttcc agggaatcgc gcacttcgcg gtcgtatttg ttcataaacg taacaatcgg
     2941 tgtatcgcgc aggcggcaga cgtttaagag cttgatggtt tgcgcttcca cgccttttgc
     3001 cgcgtcgatg accattaatg cgctgtccac ggcggttaaa acgcggtagg tgtcttcgga
     3061 gaagtcttgg tgtcccggcg tgtccaagag gttgacggtg tggtctttgt aatcgaactg
     3121 catcacactt gatgccacgg aaatgccgcg ctgcttctcg atttccatcc agtcggaagt
     3181 ggcgaatttg ccggttttct tgccttttac cgtacccgcg ctctgaatcg cgcccgaaaa
     3241 cagcaagagt ttttcagtca acgtggtttt acctgcgtca gggtgggaga tgatggcaaa
     3301 cgtgcggcgg cggcgcactt ggtcgaggat ttcttgggac atggttttct ttgcaaaaag
     3361 gttcaggccg cttttcagac ggcccggaca gtgtttgaga cggcgaaatt gtacaaaaaa
     3421 atgcctgata attcaatgtt ggaggcggtc agtgcgtgct gccgtaaatc tctttttcgt
     3481 ctttcaggac ggcatcggcg gtttcccacg cgctgccacg ccagactttg taaaagcagc
     3541 tttctcgccc ggtgtggcag gcgatgccgc cgttttgggc gatgagcatc acaatggcgt
     3601 cgccgtcgca gtcgaggcgc agtgcgcgga ctttttgcgt gtgtcccgac tcttcgccct
     3661 tcatccattg tttttggcgc gaacggctgt aatagtgggc aaagccggtt tcgacggttt
     3721 tttgcagggc ttcggcgttc atccacgcca ccattaaaat acgtttggtt tcggcatctt
     3781 gggcgatggc gcaaaccaaa cctttttcgt caaatttgac ggcttcaagc aggtttttat
     3841 ccatatttcc tttcagacgg catagtcgag gcggtcagag gcgcacttcg atgccggctt
     3901 cgcgcatagc gcgtttggct tcgcggatgg cgatttcccc gaaatggaaa atgccggcgg
     3961 caagtacggc atcggctttg ccttcggtta tgccttcaat caggtgccgg acattgccga
     4021 ccccgccgga ggcgatgacg gggatgtcga cggcttcggc aacggcgcgg gtcagcggca
     4081 ggttgaaacc ctgtttcgta ccgtccctgt ccataccggt gagcaggatt tcgcccgcgc
     4141 cgcgtttttg catttcgacc gcccattcca ccgcatccaa accggtcgga tttcgcccgc
     4201 cgtgggtaaa gatttcccag cgtgtgtttt cggggttggc ggctttggca tcgacggcgg
     4261 cgacgatggc ttgcgaaccg aaaaatccgg cggcttcgtc aattaaatcg ggacgggtaa
     4321 cggcggcggt gttgatgctg actttgtccg cgccggcatt gagcaggcgg cggatgtcgg
     4381 caacggtgcg tacgccgccg ccgacggtca gggggatgaa gacttgtccg gcaacctctt
     4441 cgatgatgtg caggatggtg tcgcggttgt cggatgaggc ggtgatgtcg aggaaggtca
     4501 attcgtccgc gccttcgccg ttgtagcgtt tggcggcttc gacggggtcg cccgcgtcgc
     4561 gcaaaccgat gaagttcacg cctttgacga cgcgcccgtc ttttacgtcg agacagggga
     4621 tgatgcgttt tgccagtgcc ataatcggat gcctttagtc gagggaatct gccagttgct
     4681 gcgcttgggc aaaatcgatg ctaccctcgt aaatcgcgcg gccggtaatc gcgcctgcta
     4741 cgccatgttt ttcggcggca cacagggcgc ggatgtcgtc caagccggtc agtccgccgg
     4801 aggcgatgac gggaatgcgg acggtttggg cgagtttgac cgtcgcgtcg atgttcacgc
     4861 cgctcatcat accgtcgcgc ccgatgtcgg tgtagatgat gctgttgacg ccgtcgtctt
     4921 caaagcgttt tgccaaatca attacatgat gcccggttac ggttgcccag ccgtcgatgg
     4981 cggccatacc gtctttggca tccagcccga caataatcct gccggggaag gctttgcacg
     5041 cctcgcgcac gaagtcgggg tttttgaccg ccgccgtgcc gataatcacg tcgtttaagc
     5101 ccaaatccaa atattgtccg atggttttca aatcgcgtat gccgccgccg agctgtacgg
     5161 ggatgtcttt ggcgacagcg gcaaggatgt ctttgatggc gggcaggttt tgcggaacgc
     5221 cggcaaacgc gccgttcaaa tctaccagat gcaggcggcg cgcgccttgt ttgaaccagt
     5281 gcagcgcggt ttcggcgggc gaatcggaaa agacggtcgc ctcttccatc agcccttgtt
     5341 tcaggcggac gcagcgtcct tctttcaaat cgatggcggg tatcagcagc ataatttttc
     5401 tccttgtgcg gggccgtgtc cggcttacca gtttaaaaag tttttcaaca tcgtcagccc
     5461 ggcatcgtgg cttttttcgg tgtgaaattg cgtggcgaat acgttgtctt tgccgacgat
     5521 gcaggcaaac ggggacgggt agtcgctttc gcccaatatg gtttcgggat tttcgggggc
     5581 gaaatagtag ctgtggacga agtaaaaacg cgtgtcttgg ggaatatctt taaacagcgg
     5641 gtggttttgg gtttggcgca cggtgttcca gcccatatgc gggactttca gacggcatcc
     5701 ctgcgggtcg cggaggtcgc gctcaaagcg tctgactttg ccgccgaacc agcccaagcc
     5761 gtcggtgttt ccttcttcac tgtggtcgaa taaaagttgc gcgccgacgc agattccgaa
     5821 aaacggtttg ttttttaagg catctttgac tgcctcgtcc aaaccgtctc gttttaatgc
     5881 cgccatacag tcgggcatcg cgccctgacc gggaaaaatg actttgtcgg cgcgggacac
     5941 gcggtcgggg tcgccgctta aaaagatttc ggtatttttt ccggcaagct gccccgccgt
     6001 ccggacggat ttcaatacgg aatgcaggtt gcccataccg taatcgataa tggcggtttg
     6061 catggcttcc tcctcttttt ttgcaatatg gctgcgattt taacaaacaa atgtgccgtg
     6121 ctgataaaaa tgccgtctga aaacgggagt ctgtcttcag acggcatagg gtttaaaccc
     6181 ggaaagccgt ttgtcagcct tccatttgtt ttgcctgaac ggcagtcagg gcgatggtaa
     6241 acacgatatc ttctaccagt gcgccgcggg agaggtcgtt gaccggttta cgcaggcctt
     6301 gcagcagcgg gccgacgctt aagacgttgg cgttgcgttg gacggcttta taggtgcagt
     6361 tgccggtgtt caggtcgggg aagaccaaaa cggttgcctg tcctgccacc gggctgcccg
     6421 gagctttgga tttgcccaca cccggcacgg ttgccgcatc atattgcagc gggccgtcga
     6481 tggcgaggtc ggggcgtttt tcccgggcaa gtttggttgc ttcgatgacg gtatcgacat
     6541 cggggccgct gccggagttg acggtggagt aggaaatcat cgccactttc gggtcgatgc
     6601 cgaaggcttt tgcggaatcg gcagactgga tggcgatgtc ggcaagctgt tgcgcggtcg
     6661 ggttcggatt aaccgcgcag tcgccgaaga cgaggacttg gttgggcagc agcataaaga
     6721 atacgctgga cacgaggctt gcgcccggtg cggttttaat cagttgcaaa gcggggcgga
     6781 tggtgttggc ggtggtgtga accgcaccgg ataccaaacc gtccacatca ttttgcgcca
     6841 tcatcatcgt accgagtacc acggtgtctt gcagttgctt gcgcgcgtct tcgggtgtca
     6901 ggcctttgga tttgcgcagt tcgcacatcg gctcgacgta ttgttcgacc aatgaggcgg
     6961 gatcgatgat ttccaaagag tcgggcaggc tgatgccgcg ttctttggca acggcttcga
     7021 cttcttcgcg tttggcaagc aggacgcagc gggcaatgcc tttttcgtgg cagatggcgg
     7081 cggcttggac ggtgcggggt tctgcgccct caggcaggac gatgcgtttg tcggcttggc
     7141 gggcgaagtc gatcaggttg tagcggaatt gcgccggcga caggcgtttt gcttcgcggc
     7201 ctgccaatac ggatacgtct ttcagcgcgt cgctcgaacc gaagaaggtc aggccggttt
     7261 tttcggctgc cgcttcggca acggaggctg ccgcgccgtc cacgacaaaa ccttccaata
     7321 cgcccggcgc ggcggcgaag aactgtttgg caaggttcac ccgatttgcc agttcgtcgg
     7381 catcggcgtt gtcggaacgg acggcgaaga cggctgccgc gtcaagggac aatgccagtt
     7441 cgacgttttt gcctgcgagg tagattttgt cggcatcggg cgcgatgcct tcgatgacga
     7501 ggttggcggc atcgagtgcg gcaactttgc cgaccagtgc gtcgaaccag tcgtcgcttt
     7561 tgccttgcgc gagcagggtt tcggcggttg cgtcaacggc ttggaaaatt tgtgcgtcca
     7621 gtgcttttgc aaaggcttgt gcggcggcgg aggcgtccag tccggcagat acgggtacga
     7681 tgagtacttt tgccatgata tatcctttcg tatgctgcgg tgtgcggcat atgtggttgg
     7741 aaggggcggc atataggcag aaacggctgc ctgcgtgccg tgcgtgccgt gtttggcttg
     7801 aggcgcgcag gttgaatata gcaaacaaat tctgtttcca acaagataaa tatccgcagg
     7861 cttgtggatg ctgccgcctt tcagagggta tttccgggga agaacagggc gggaccgtcc
     7921 aaatggagga cggcggaaat gccgtctgac agggtggggg cggaagggag gttgagcgtg
     7981 aggacggttt gtccgacccg gaggctgatt tcggtatgcc gcgctttggg cgtggttttg
     8041 agaaccacgg cgtgaatgga ggcggtgggt gcggaatggg ggtgaaggct gaactgttcc
     8101 ggacggatga gcagtgtgcc gcgtgtgcct gcgggtgcgc cgctttgaac gggcaggcgg
     8161 cccaatccgc aatcggcggt gccgtcggcg ttgagcgcgg cggggaacac gatgccttcg
     8221 ccgataaaca gggcggcatc aaggtcggca ggttgtcggt acaattcgtg agggcttgca
     8281 gtttggagga tgcgcccctg tttcatcacg gcaatccggt cggcgtattg cagggcttct
     8341 tcgcggtcgt ggctgacgaa aacggcagat ttgccgttgg cgcgcagggc ggcaatcatg
     8401 tcttcgcgaa tctggcggcg caactgttcg tccagcgcgc tgaagggttc gtccaacaaa
     8461 atcagttcgg gatcgggtgc gagggcgcgg gcgagggcga cgcgctgttg ctgtccgccc
     8521 gaaagttcgt gcggatagcg tccggcaagt tcggaaatgc cggtcaattc caacatagct
     8581 tcgatgcgct gccgctcttg cgccgtcttg cctttgccgt tgcccagccc gtaggcggtg
     8641 ttgcggtaaa cggtcaggtg ggggaacagc acaccttcct gtacgacata acccaaacgg
     8701 cgttcgcgga cggggaggtt ggtatttttc gagaagatgg ttctgccgga aagcgaaatt
     8761 tcgccaaaat cgggttgttc aaaaccggca aggcagcgta aaagggtggt tttgccgcag
     8821 ccggacgcgc cgacgataaa gaggatttcg cccgggtcga ggctgagcga aatgtcgttt
     8881 aaaactgggg tgttttgaaa acttttggac aggtgtccga tgtgcagggc ggcggtcatg
     8941 gcggtacttc ctcaagctgt tatttgaagg cgtatttctt cagcaggaat acggggatgc
     9001 cggaaaataa taccagcatc agcgcgtaag gggtggcggc ggcgtattgt gcgtccgatg
     9061 tgtattccca aacggcggtg gagagtgtgt ggacatcgtc ggtggtcagc agcagggtgg
     9121 cggtcagctc tttcatcagt ttgaggaaga cgagtgcgaa tgcggcggta atgccgggca
     9181 ggatggacgg cagtaccaac gtcctgaaaa taaagaagtg tccgcgcccc aatgttgcgc
     9241 cgacctgttc catccctttt gggagttgtt ccaaggaagt cctcagggtg gtttgcgcca
     9301 tcggcaggta aagcatgaaa taggcaagga tgacgacgat aaaggtttgg taaacggcag
     9361 gggtgtagtt gatgctgaaa taaaccaagg atagggcgat aaccaaaccg gggacggcgt
     9421 gcagtaaaaa cggcagcctg tctatccaaa cggttaaaaa attgcgatag cgaaccgatg
     9481 cccaaacaag gggcaaggca cataatatag tcaaaatcgc acctaaagcc gatacgctta
     9541 aggaacggat aaaggcatca aatacggata cgagcgcgaa tgtgccggaa gtgccgacca
     9601 tcatccaatg tatcaatacg ccaaagggga taataatgcc caaagtcaac aagctgctta
     9661 aaaaaacaat cgcgccaatc tgaccgggca gtttgagggt tttgacggga taaggacggg
     9721 caacgccttt gccgctgtgg taaatcttgg ctttgccgcg aaatatgctt tctccaaata
     9781 cgacgatgcc gcacaccgcc attaaaacag cggaaagcag ggcggcggta ttgttgttgt
     9841 aggacatttc gtattcttgg aaaatggcgg tggtaaaagt ggggtagttc aaaatggata
     9901 ccgcgccaaa ttcgaccagc atatgcaggg caatcagtaa cacgctgctg ccgatggcgg
     9961 gtttgagctg ggggaggatg gcggaaaaaa aggtttgcag gcggcttttg cccaaggaca
    10021 ggctgacttc ttcgtaagac aggctgatgc gtttgagtgc cgcctcgacg ggcaggtagg
    10081 cgagcgggaa cgaggacagg ctcataatca tcactgtccc ccaaaagcct tcgacacgga
    10141 aggtcaggct gatccaggtg aaacagctga caaatgcggg gatgcacaaa ggcagggtga
    10201 ttgccgtctg aaaaaaggtt ttgccgaaga agcggtaacg ttggaacaaa agggcgcagg
    10261 caatgcccaa aacaatggaa atcagggtaa cgcccgccat catcgtcaag gtgttggaga
    10321 gcaaatccca catacgcggg cggaacaaca gttcgacggc gcggttgatg ccgacctgcc
    10381 acgaacgcat agcgacatat aaaaaaggca gggtaagcgg tagggcaatc agtaggatga
    10441 ggccggtaag ccaaatgggt atttttttag gagacatagt gttttttatc ggcaaaacgg
    10501 gcggacagta taaatgtcca cccgtttgac aatccgaaaa cggcttattt cataccggct
    10561 tgctcaagca gccgggtggc gtgttctttt tcggaaacag tggtggcgga cacttggggt
    10621 gcttccaact tggcgatggg ttccaaattg aaagtggata ccacgtgcgg attcaaagga
    10681 tattcggcac ggacggcggt cagggcgcgc tgtccttcct tgctggcgag gaaggcgacg
    10741 aattttttcg cctcatcctt gttttgggag gattttaaca cggctgcgcc ggaataggta
    10801 acgagtgcgc cgggatctct gtggcggacg aaattcaggc gggtgtggac attttgtacg
    10861 cctttttcac gcgcaaaagc gtgccagtag tagttgttga tgagggcggc atcgatttcg
    10921 ccgttttcaa ccgcttgaag ggcgacggag tttttagcgt aaggcttgcc gtattctttc
    10981 agacctttga gccatttcaa tgcggccgct tcgcctttca gtttgacgat ggcgacaacc
    11041 tgttccaaga acgcgccgga agtgggggcg taaccgatgc ggtttttcca tttcggcgtg
    11101 gcgtaattca ggacggattt ttccaaatct ttttcagaca gtttgcgggt gtcgtaaacg
    11161 acgacgcgcg aacgtccgct cagtgccacc cagtcttttt tggcggcaac cggcacgccc
    11221 ttgccgcgtg tttcgttgat ggtggaggcg ggcaggggct ctaggaggtt ggcggcggaa
    11281 agggtggcga gtgccgggat ttgttcggaa tagaatacgt cggcggggct tcggctgcct
    11341 tcttctttga tttgaccggc aagctggtcg cctttggcgc tgttgagttt gactttgatg
    11401 ccggtagccc gggtaaaggc atctgcaacg gcttgtgctg cttctttgtg ttggccgttg
    11461 tacacggtaa tgtctgccag cgcgggggtt gcggcggtca gggctgcggc aagcagtgcg
    11521 tatcggatag atgttttcat atcgattttc tcctaatgaa tgagagtgta taccttgtta
    11581 agacataacg gtgtgtagtg tattccttct tttttataaa tgcaaataat tattttttaa
    11641 atttgttgtt gtccgatccg gttattgttt gttctgactt gtattttttc cgtgagtctc
    11701 gcccgtaagg cggaagtggc gggcaatgcg tggcggaatg tgggtaaagg cggcattttg
    11761 atttgtcgga atgcttgaga acccctctct ttaaaac
//
LOCUS       DAWIVB010000042        11773 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWIVB010000042 DAWIVB010000000
VERSION     DAWIVB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.042"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(328..1380)
                     /locus_tag="WIX60_000852"
     CDS             complement(328..1380)
                     /locus_tag="WIX60_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ5305141.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKVAKVAKV
                     AKVAKVAKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLP
                     KDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKNLSPAQR
                     RDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILI
                     KADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPA
                     FEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQAT
                     VNLLRDLHSGAYVDIR"
     gene            complement(1485..3893)
                     /locus_tag="WIX60_000853"
     CDS             complement(1485..3893)
                     /locus_tag="WIX60_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ5305142.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRN
                     VSEPMQPADLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VKVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIWGETLTYNLEQ
                     QTGEAHNVRMETEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLFVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTDRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTVNSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYITAHSLSAGR
                     NKRP"
     gene            3963..4967
                     /locus_tag="WIX60_000854"
     CDS             3963..4967
                     /locus_tag="WIX60_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ5305143.1"
                     /translation="MQRQIELKNWLQTVYPDRSFELAFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDIVNVPQILHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(5024..5722)
                     /locus_tag="WIX60_000855"
     CDS             complement(5024..5722)
                     /locus_tag="WIX60_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ5305144.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLSNPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            5926..7464
                     /gene="murJ"
                     /locus_tag="WIX60_000856"
     CDS             5926..7464
                     /gene="murJ"
                     /locus_tag="WIX60_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ5305145.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLSFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRKHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHTGGMQKAAQLFILIVIGGCLYFASLAALGFRPRHFK
                     RVES"
     gene            7567..8523
                     /locus_tag="WIX60_000857"
     CDS             7567..8523
                     /locus_tag="WIX60_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ5305146.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRDRGWHGVVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGKKALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPIPDEVSEAV
                     TLFQPAHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNSR"
     gene            8574..9356
                     /gene="trpC"
                     /locus_tag="WIX60_000858"
     CDS             8574..9356
                     /gene="trpC"
                     /locus_tag="WIX60_000858"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ5305147.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNTEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRLDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHNETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALESKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRAGDIEAEVGKLF"
     gene            9421..11721
                     /gene="recQ"
                     /locus_tag="WIX60_000859"
     CDS             9421..11721
                     /gene="recQ"
                     /locus_tag="WIX60_000859"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ5305148.1"
                     /translation="MHRLTAKQILHEVFGYPEFRGKQESIVNALAGGRSLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADEAREIAD
                     RLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNILRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVYGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         2400 a         3269 c         3373 g         2731 t
ORIGIN      
        1 cgcgggaatg acgatggaaa gattgttgtt gcttcggata aatttctgca gttttaaata
       61 accggattcc cgcctgctcg ggaatgacgg cataggtttt tttgtttttc cgataaatta
      121 ccacaaccca aaatcccgtc attcccgcga cggcgggaat ccagtccttt aaactccagc
      181 cattcccgat aaattcctgt tacttttcgt ttctagattc ccgctttcgc gggaatgatg
      241 aatgacggtg taaaagtaac tcgaaatcca aaaagccata ccgcccggat ttttgccgat
      301 cggtatggct ttttgcttca aaccgcctta gcggatgtcg acatacgcgc cggaatgcag
      361 gtcacgcaac aggttgacgg ttgcctgttc ggctttttgt tggaagatgt attgccgcac
      421 ggaattgcgg atacgttcct gaggtgtgcc ggcatcgcgc acttcgttca atttgatgat
      481 atgccagccg aattgggtgc ggacgggcgc gccgacctgt ccgggtttga gcgcgtggac
      541 ggcttcttca aaggcgggaa ccatcacgcc gtcggcaaac cagcccaaat ctccgccgtt
      601 gcccgcgctc gcgtcttgcg aatattggcg cgccaggctg gaaaagtctg tgccgctgcg
      661 ggcctctccg tagattttgc ggatggtgct ttccgcgccg acggcggcgt tttcgctgtc
      721 ggctttaatc aggatgtgtt gggcgcggta ttggcgcaac ggtgcgcctt cgggcagggt
      781 gatgccttgt ttttgcgcct gctcgaggaa ggcatcgatt tcagcttcgc tcacgcggct
      841 gttctgcatc actgcctgct ggcggacttt ttcggcaatg atgttgtcgg caaaatcgcg
      901 gcgttgggcg gggctgaggt ttttgagggc gggatttttt gcgacgacgg catcgatttc
      961 cgcttcgctt gcttgaatgt tgcggcgttt gcccgcctgt acaatcaggg attggttgac
     1021 aagctgcatc agcacctgtc gggacagctc ggattcgctt atctgcgcgt ctttgggcag
     1081 gttggctttg gcttcggcaa cggcttcggc aagccggcgg cgcgtgatga cttcgttgtc
     1141 ggcaacggcg gcaatgccgt ctgaaaagcg gataccgccc tgctgttgtg cgggtgcggc
     1201 ttctttttgc gccgtggcgg caactttggc aactttggca actttggcaa ctttggcaac
     1261 tttggcaact ttggcagctt tggcagcttt ggcggatgcg gtttttgcct tttgcggtgc
     1321 ggcgtggaca tcggctgctg ccagcaatgc ggcggcaatc atcagggctt tgattttcat
     1381 cattctttcc ttatcgtttg aatcctgcct ttgatggcgg gtttggggtt gtcggtggaa
     1441 tgccgtcttg tgcgggaacg gtgctcctgc caggtttccg acggtcaggg tcgtttgttg
     1501 cgtccggcgg aaagagagtg ggcggtgata tagccgggaa cggcgacatc catcctgtct
     1561 gcggggtttc tgccgacact gctgaggtct ttcaactgaa gtgagaaaaa gacagcgttt
     1621 ttgtaggtgt tttcgccggt aacgtagcgt tgggcgtaca cgcccgcgcc ccagcagccg
     1681 caactgcttt tgtattccgc acccgccagc acctctatcg gttttttggc ttcaaaaccg
     1741 tagttgtaac ggacgacggc cgacaggttg cgcgtcagcg gccattgtgc ggacaggtcg
     1801 agctggctga gtttgtcgta aaaataggaa ccgtcggact tcaggtagat tttttcgttg
     1861 cgcccgtatt tgtagcgggc gttcagcact ttgccctgtg cgggacggta gcttgcaccg
     1921 acggcgtagt tctcggcgcg tttgtcgttt tggttgtagt ggatgctgct gtcgaggatg
     1981 aagcggctgc cgatgctgcc ggaggcaaat gccacccagt cggaacggtt gcgcggtttt
     2041 ttgccgacgc tgccgtcaag catcaccgca tcatccttga aatagaattt ctgaccgatg
     2101 ccggcgcgga aacgctcttc ccccgtcgcg ccgtccaaaa tacggctttg cacggcggcg
     2161 gaaaggctgt ttacggtgtt aatcctgtcg ttgccgtaat agaggttttc gcgaaagagc
     2221 tgcccgtagc cgaagctgct ttccgacgaa tcgaaattgg gcaggtcgtt ttgggatttg
     2281 gcaggaatat agttgtagaa caggcgcggc tcgagggttt gcaggactcc gccgccgaac
     2341 atccgcgtat tgcgttcgaa ggtcatgccg ctgtcgatgt tgacgatggg cagagtgcgg
     2401 ctgacgcgtc gggcttcttg gctgccgaag cggttgaggc tgtaataggt ggcgtgcagt
     2461 ccgagtttgg gacggacata gccccagctg ttgctgaaat cccatttgat gtcgggatag
     2521 acgacgaggc ggctgccgtc ttggcggctg tcgtggctga agcgggtaaa ttgtgcggac
     2581 acgccgattt gcgccctgtc ggtgtttttg cgccaatcgg cggaaaggcg cggcatcagg
     2641 gcatacggtt tgtctttgta gccgctttgg tttgccagcg tctggtattt cagaaccgaa
     2701 aggccggcat tcaggctgcc gcccgccgcc ctgccgccat aatccagcca tacacggcgg
     2761 ttgaggttga cgttgccggc gatttctttg ttgccgtaaa agtcacggta gtagccgctg
     2821 tcggagactt ggttgaaatc gacacccgcc tgaagcgtgt cggaaatgtc gtgccggtgc
     2881 tgccatttcg cctgatagcg gttattcctg ccgctttttt tgtcgtgcgg cagccaggtc
     2941 aggtcggact ggccggcata atccggccgc aggtagcgta cctgcccgtc aaagaccgcg
     3001 ccgcgttcgc cgatcacgcc gggcgcgaac gtggcatcga gattgggggc aaggttgaaa
     3061 taatagggaa cggaaaggga aacgccgtcc gaaccggcgg acagtgaggg aacaaacagg
     3121 ccgcttttgc ggttgccgtc aagcgggaag tccgcccaag gggtgtagaa aatgggaacg
     3181 ccgccgaaca cgaaggcggc gtgtttggca acgcctatgc ctttttcccg atcggcttcg
     3241 acggaagcgg ccttgacata ccagccggca tcgccggcgg aacaggtgtt aaattgggtt
     3301 tccgtcagtt tgtaatgccc ttcgcccaac atttcggcgg tgcggctgac gctttgcagc
     3361 cgccgtccgc cttgttcggt ttccatgcgg acgttgtgcg cttcgccggt ctgctgctcg
     3421 agattgtagg tcagggtttc gccccaaatc agcgtaccgt cctgttggag ggcgaaccgg
     3481 tcgcctacgg taacggtgtc gcccgactgg tcgtaatccg cccaatcggt attgaggact
     3541 gcgccgtccc gttcgatgat aacgcttcct tccgcgcgta ccttgacctg cgactgccct
     3601 tccaccttgt cggcgacaat gcgcgtatag tcttcgggga tggatgcttc gccgctgcct
     3661 cggacggcgg cttcggttct ctcggggctg ccgctttcgt tactgcaaaa caggcaggtc
     3721 gaaccgaggc tcaggtcggc aggctgcatg ggttcggaca cgttccgaac gcttcctccg
     3781 gcggtcggat tgtccgtttc ctccgccgca acggcatcgg cggcggcgca atgcgtgccg
     3841 aaacagaagc ccaatgccag caccagtggt ttgagtgaaa ataaacgagc caaaatcgcc
     3901 cctcaagtcg gtttgccagt tagaatagcg tttattgtaa cctgaaatgc tttagtactg
     3961 ttatgcaacg acaaatcgaa ctgaaaaatt ggcttcagac cgtctatccc gaccgcagtt
     4021 tcgaactggc ctttgcggcg gcggatgccg atttccgccg ctatttccgt gcaacgtttt
     4081 cagacggcag cagcgtcgtc tgcatggatg cgccgcccga caagatgagt gtcgcacctt
     4141 atttgaaagt gcagaaactg tttgacatag tcaatgtgcc gcagatattg cacgcggaca
     4201 cggatttggg gtttgtggta ttgaacgact tgggcaatac gacgttttta accgcgatgc
     4261 ttcaggaaca gggcgagacg gcgcacaaag cgttgctgct ggaggccatc ggcgagctgg
     4321 tcgaattgca gaaggcaagc cgtgaagggg ttttgcccga atatgaccgt gaaacgatgc
     4381 tgcgcgaaat caacctgttc ccggaatggt ttgtcgcaaa agaattggga cgcgaattaa
     4441 cattcaaaca acgccaactt tggcagcaaa ccgtcgatac gctgctgccg cccctgttgg
     4501 cgcagcccaa agtctatgtg catcgcgact ttatcgtccg caacctgatg ctgacgcgcg
     4561 gcagaccggg cgttttagac ttccaagacg cgctttacgg cccgatttcc tacgatttgg
     4621 tgtcgctgtt gcgcgatgcc tttatcgaat gggaagaaga atttgtcttg gacttggtta
     4681 tccgctactg ggaaaaggcg cgggctgccg gcttgcccgt ccccgaggcg tttgacgagt
     4741 tttaccgctg gttcgaatgg atgggcgtgc agcggcactt gaaggttgca ggcatcttcg
     4801 cacgcctgta ctaccgcgac ggcaaagaca aataccgtcc ggaaatcccg cgtttcttaa
     4861 actatctgcg ccgcgtatcg cgccgttatg ccgaactcgc cccgctctac gcgctcttgg
     4921 tcgaactggt cggcgatgaa gaactggaaa cgggctttac gttttaaacc caatcaaaat
     4981 gccgtctgaa aaccaagttt cagacggcat ttttcaaacg ggcttactgc gcggcttttt
     5041 gttcttcacg tactttgtcc gccaaaaggt cgatggtgtt cataccggac tcccagtcgg
     5101 caaattcgac tttatatttg ccgccgacga taaccgtggg cgtaccgtcg atttggaagg
     5161 tttcggtcag ctcctgcatt ttgtcggcgc gcgcctggct ttcgggagat tcgtaagcgg
     5221 caaggacttt tttgccgtca aaggcggttt gttcgcccag ccattttttg aggacttccg
     5281 gctcttgcag cttgattttt tggttgacca tcgcatcgaa aatatggctg ttcgccacat
     5341 ctttgctgtc ggcggcagcc atatcgacgg cggcggcgag gcgtgccaac ggcagcattt
     5401 ctttctgcca gacgacatgc tcggtacgca ggtacatatc gtctttaaaa gacttggcgt
     5461 gtttgctcaa aacaggttcg aggtgggcgc agtgcgggca gaaatagccg aaaaactcaa
     5521 ggacttcaac cttgcctgcc tgctgttggg gaatcgggtt ggaaaggaca gtatagtttt
     5581 gcccttcgac cagccctgct ggggcggcgg ctgccgaagc ggcaggcgcg ctgtcggcgg
     5641 ggacgctggt ttggactttg ctgtcgcacg cggcaagggc gaacagggcg gcaacgccga
     5701 gggcaaggtg tctggatttc atacggctct ccgtgatgtt ggaaataaat cggatattgg
     5761 tattttattg tattttcgcg tgttgataca gtttgcctcc ggaaaaggaa gctttcgtat
     5821 cgggaaaccg cttcagacgg catcaaaccc gatgccgtct gaagtggttt ctgtcgtaca
     5881 atacgcgccg ttgccccaaa cgggtacgac tgttgaggaa caatgatgaa tatgcttgga
     5941 gctttggcaa aagttggcag tctgacgatg gtgtcgcgcg ttttgggatt tgtgcgcgat
     6001 acggtcattg cgcgggcatt cggcgcaggc atggcgacgg atgcgttttt tgtcgcgttc
     6061 aaactgccca acctgctgcg ccgcgtgttt gcggaagggg catttgccca agcgtttgtg
     6121 ccgattttgg cggaatataa ggaaacgcgt tctaaagagg cgacggaggc ttttatccgc
     6181 catgtggcgg ggatgctgtc gtttgtactg gtcatcgtta ccgcgctggg catacttgcc
     6241 gcgccttggg tgatttatgt ttccgcgccc ggttttgcca aagatgccga caaatttcag
     6301 ctctccatcg atttgctgcg gattacgttt ccttatatct tattgatttc actttcctct
     6361 tttgtcggct cggtactcaa ttcctatcat aaattcagca ttccggcgtt tactcccacg
     6421 ttcctgaacg tgtcgtttat cgtattcgcg ctgtttttcg tgccgtattt cgatccgccc
     6481 gttaccgcgc tggcttgggc ggtttttgtc ggcggtattt tgcagctcag ctttcagctc
     6541 ccttggttgg cgaaactggg ctttttgaaa ctgcccaaac tgaatttcaa agatgcggcg
     6601 gtcaaccgcg tgatgaaaca gatggcacct gcgattttgg gcgtgagcgt ggcgcagatt
     6661 tctttggtga tcaacacgat tttcgcgtct tatctgcaat cgggcagcgt ttcatggatg
     6721 tattacgccg accgcatgat ggagctgccc agcggcgtgc tgggggcggc actcggtacg
     6781 attttgctgc cgactttgtc caaacactcg gcaaaccaag atacggaaca gttttccgcc
     6841 ctgctcgact ggggtttgcg cctgtgcatg ctgctgacgc tgccggcggc ggtcggactg
     6901 gcggtgttgt cgttcccgct ggtggcaacc ttgttcatgt accgcgaatt cacgctgttt
     6961 gacgcgcaga tgacgcaaca tgcgctgatt gcctattctt tcggtttaat cggtttgatc
     7021 atgattaaag tgttggcacc cggcttctat gcgcggcaaa acatcaaaac gcccgtcaaa
     7081 atcgccatct ttacgctcat ctgcacgcag ttgatgaacc ttgcctttat cggcccactg
     7141 aaacacgtcg gactttcgct tgccatcggt ctgggcgcgt gtatcaatgc cggattgttg
     7201 ttttacctgt tgcgcaaaca cggtatttac caacctggca agggttgggc agcgttctta
     7261 gcaaaaatgc tgctctcgct cgccgtgatg ggaggcggcc tgtatgccgc ccaaatctgg
     7321 ctgccgttcg actgggcaca caccggcgga atgcaaaagg ccgcccagct cttcatcctg
     7381 attgtcatcg gaggctgtct gtatttcgca tcactggcgg cactgggctt ccgtccgcgc
     7441 catttcaaac gggtggaaag ctgaccgatg caccgccttt tgccggaaaa cgctttttcc
     7501 gcgcgtccgt gccggacggg ctgccgcatc atcaatgccg tctgaaaaca agaaaaccga
     7561 taccttatga ttctgacacc gccggacaca ccgtttttcc tccgcaacgg caatgccgac
     7621 acgattgccg ccaaattcct gcaacgcccc gcgcccgcat accgccgaga gctgcttccc
     7681 gacagcacgg gtaaaaccaa agtcgcctac gacttttcag acggcatttc gcccgatgcg
     7741 ccgctggtcg tgctgtttca cggtttggaa ggaagcagcc gcagccatta cgcggtcgaa
     7801 ctgatgcttg cggtacgcga tcggggttgg cacggcgtag tcgtccattt ccgcagctgc
     7861 ggcggcattg ccaacaccgc tccggtgttc taccacttgg gcgataccgc cgaaattgcc
     7921 tttactttgg acacgcttgc cgcgcgttac cgtgaaatat acgccgtcgg cgtatcgctg
     7981 ggcggcaacg cgctggcaaa atatttgggc gaacagggca aaaaggcatt gccgcaagcc
     8041 gctgccgtca tctccgcccc cgtcgatgca gaggcggcag gcagacgctt cgacagcggc
     8101 atcacgcggc tgctctacac gcgctacttc ctccgcacac tgatacccaa agcacggtcg
     8161 ctccaaggtt ttcagacggc atttgccgca gggtgcaaaa cactgggtga gtttgacgac
     8221 cgtttcaccg caccgctgca cggctttgcc gaccggcacg actactaccg ccaaacttcc
     8281 tgtaaaccgc tgctcaaaca cgttgccaaa ccgctgctcc tgctcaatgc cgtcaacgac
     8341 cccttcctgc cgcccgaagc gctgccgata ccggacgaag tgtccgaagc cgttaccctg
     8401 ttccaacctg cacacggtgg tcatgtcggc tttgtcggca gcaccggcgg caggctgcac
     8461 ctgcaatggt tgccgcagac cgtcctgtcc tatttcgaca gcttccgcac aaacagtcgt
     8521 taacggtttg atgctaatat tccccccttt cccagacgaa tacggaaccc gacatgaccg
     8581 acatcctcaa taaaatcctt gccaccaaag cacaggaagt tgccgctcaa aaagccgccg
     8641 tcaacaccga acacatccgc gcacttgccg cagaagccgc gcccgtccgc agcttcatcg
     8701 attcgatacg cggcaaacac cgcctaaacc tgcccgccgt cattgccgaa atcaaaaagg
     8761 caagcccgag caaaggcctg atccgtctgg acttccgacc ggccgagatt gcacgcgcct
     8821 atgaaaacgc cggagcggcg tgtttgtccg tactgaccga cgaaccctat ttccaaggtt
     8881 cgcccgaata cctcaaacaa gcgcgcgaag ccgtatcgct gcccgtgctg cgcaaagact
     8941 tcatcatcga cgaatatcag gtttatcagg cgcgcgcatg gggggcggat gccgtcctgc
     9001 tgattgccgc agcactggaa caggaacaat tagaacgctt tgaagctgtg gcgcacgaat
     9061 tgggcatgac cgtcctgctc gagctgcaca atgaaaccga attggaaaaa tgccgcaacc
     9121 tgaccacgcc gttgtggggc gtaaacaacc gcaacctgag gacttttgaa gtctccctcg
     9181 accaaaccct gtcgctgctg cccgcgctgg aaagcaaaac cgtcgtaacc gaaagcggca
     9241 ttacgggtaa ggcggatgtg gaatttatgc aatcgcgcgg cgtgcacacc ttcctgatcg
     9301 gtgaaacgtt tatgcgtgcc ggcgatattg aagcagaagt gggcaaactc ttctaaatcc
     9361 cgatttcaga cggcatattg ccgccgaccg atcaacatcc ttaccgagca cggaaccatc
     9421 atgcaccgac tgaccgccaa acagattttg cacgaagtat tcggttatcc cgaatttcgc
     9481 ggcaagcagg agtctatcgt caatgctttg gcaggcggaa ggagtttgat ggtgctgatg
     9541 ccgacgggcg gcggaaagtc tttgtgttac cagattcccg cgctgatgcg cgaaggcgtg
     9601 gcggttgtcg tatcgccgct gattgcgcta atgaacgacc aagtggcgaa cctgcacgcc
     9661 gccggcatcg aagcggcggc agtcaacagc ggcacatcgg cagacgaggc gcgcgagatt
     9721 gccgaccggc ttgcccaagg ccgtctgaag ctgctttatg tcgcgccgga acgcttggtt
     9781 accgaccgct ttttgcgttt tctcgaccaa caaaccgtca gtctgtttgc cattgatgag
     9841 gcgcattgcg tcagccaatg gggacacgat ttccgccctg aatatcaaca gctcggcatg
     9901 cttgccgaac gctatccgaa cgtcccgcgc atcgctctga ccgctaccgc cgatgccgcc
     9961 acgcgcgccg acatcaagca ttatctgcac ttggacgatg cgcccgaatt tgtctccagc
    10021 tttgaccgtc cgaatattta ttatcaggtt atcgaaaaaa acaacggcaa aaaacaattg
    10081 ctggatttca tccgcaaaga aatgacgggg caaagcggca ttgtgtattg cctaagccgc
    10141 aaaaaggtgg aagatgtggc gcagtttttg cgtgaaaacg gattaaacgc gattccgtat
    10201 catgctggtt tgagcatgga cgtacgcgag gaaaaccaac gccgctttac gcatgaagac
    10261 aatattatcg tggtggcgac cgtggcgttc ggcatgggca tagacaaacc cgacgtgcgc
    10321 tttgtcgccc atctcgatat gccccagagt gtcgaacatt tctatcaaga atcggggcgc
    10381 gccggacggg acgggctgcc ggccgcaagc tggctgtgtt acggtttgaa cgattgggtg
    10441 ttgctgcgcg aacggattgc cgaaggcaac agcgacgagg tgcaaaagca aatcgaaatg
    10501 caaaaactcg atgccatgct tgccgtctgc gaaaccgccg cctgccgccg cgtactgctg
    10561 ctcaaacatt tcggcgaagc atccgaaccc tgcggccatt gcgacaactg cctgcatccg
    10621 cccgtacggt ttgacggcac ggtgttggtg caaaaattac tcagctgcgt gtaccgcgcc
    10681 ggacaacgtt ttgccgccgg ttacatcacc aacattttac gaggtaaaag cgacgattgg
    10741 atacgcggca accggcacga acaactgtcc acattcggca tcggtacgga gttgtccgac
    10801 aaagaatggc gcagcgtcat ccgccagtgt atcagcctcg gctacctcac cgtcaacatt
    10861 acccgatatc aggcattgca actgaccgaa gccgccaaaa aagtcctcaa aggcgaaacc
    10921 gaagtgatgc tgcgtccgct caagcgcgac aagcccgccg cccgcaccct caaagacaac
    10981 tggctgcgta ccgaacgcga agaacgcctg tggcaggcat tgcgcgtttg gcgtatgaaa
    11041 caggcagaag ccgaaggcat ccccgcctat atgattttcg gcgacaaaac cctgcgcgac
    11101 cttgtcgaaa aaatgccgca agacctcaac ggtctgcacg acatctacgg tttgggcgaa
    11161 gccaaaatcg accgtttcgg acacggcatc ctcgaagtct gccgcaacgc cgccggcttc
    11221 agccgcgacg cggtcatccg tccgcaaacc gaacgcgaac aacaactgcg tcaaaaactc
    11281 gaagcctggc ggtatgaaca ggcaagggcg gaaaactgcg ccctgcatgc cgtcctctcc
    11341 gacgaaagcc ttgccgatat gcttgccgat acgcccgaaa ccgaaaccga cctcgaaggc
    11401 gtgtacggct tgggcagcgt acgcgccgcc aaatacggac gggacatcct cgccgtctgc
    11461 cgtccgtttt cagacggcat cgatgaaacc gccaaacgca aacgccgcct gatgcgcgcc
    11521 ctgatccaat ggtgcggcga aacggcaaaa cacgaacagt ccgaacccta ccgcattctc
    11581 agcaaagccg cactgcgcgc cattgccgcc aaacagccgg aaggcttggc ggagcttgcc
    11641 gccgtatacg gcgtaggcga agaaaaagcc gcacgttacg gtgcggcggt gttggcggta
    11701 ttggaacgaa atgccgtctg aagcccgtta ccaagtttca gacggcattg cctctattta
    11761 aaaattcctg ttt
//
LOCUS       DAWIVB010000043        11517 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWIVB010000043 DAWIVB010000000
VERSION     DAWIVB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.043"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            197..1039
                     /locus_tag="WIX60_000860"
     CDS             197..1039
                     /locus_tag="WIX60_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ5305149.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1068..1532
                     /gene="nrdR"
                     /locus_tag="WIX60_000861"
     CDS             1068..1532
                     /gene="nrdR"
                     /locus_tag="WIX60_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ5305150.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSTELADMVLKELLEHNTEAAVRFAALHKHFDNPADFASWLAQAVKTDRKA"
     gene            1564..2673
                     /gene="ribD"
                     /locus_tag="WIX60_000862"
     CDS             1564..2673
                     /gene="ribD"
                     /locus_tag="WIX60_000862"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ5305151.1"
                     /translation="MFSDTDISMMENALDLADLGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALVRAGVSRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHNIKTVFRKNGNAF"
     gene            2868..4289
                     /locus_tag="WIX60_000863"
     CDS             2868..4289
                     /locus_tag="WIX60_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ5305152.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYATADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLAEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFAFKTESSCRLWQPLKRLPLYLHTLFCLTSSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            4457..5698
                     /locus_tag="WIX60_000864"
     CDS             4457..5698
                     /locus_tag="WIX60_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ5305153.1"
                     /translation="MSKFFKRLFDIVASASGLIFLSPVFLILIYLIRKNLGSPVFFFQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDGERLTPFGKKLRAASLDELPELWNILK
                     GEMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPPFTGKRKLAVVGAGGHGKVVADLA
                     AALGRYREIVFLDDRAQGSVNGFSVIGTTLLLENSLSPEQYDVAVAVGNNRIRRQIAE
                     KAAALGFALPVLVHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLNAFVHISPGAHLSGNTHIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVAGNPAKPLPRKNPETSTA"
     gene            5831..7006
                     /locus_tag="WIX60_000865"
     CDS             5831..7006
                     /locus_tag="WIX60_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ5305154.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYNHEHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKRLPEWTARRRENAAKLAESLG
                     KFSSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVGELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLTNAVELGDTSLMFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            7054..8964
                     /gene="pglD"
                     /locus_tag="WIX60_000866"
     CDS             7054..8964
                     /gene="pglD"
                     /locus_tag="WIX60_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ5305155.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPAFEKQIAEGGPITLTHQDITRYFMTIPEAAQLVIQAGAMGKGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            9020..10312
                     /locus_tag="WIX60_000867"
     CDS             9020..10312
                     /locus_tag="WIX60_000867"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ5305156.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     AEVNAVFADVFSKLAAEHAVENIGNYSNPQGDAVLIDALTAFLNREYSWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIATPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(10364..11200)
                     /locus_tag="WIX60_000868"
     CDS             complement(10364..11200)
                     /locus_tag="WIX60_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ5305157.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADKLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIQAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCKPVWEHFS
                     RY"
BASE COUNT         2562 a         3671 c         2957 g         2327 t
ORIGIN      
        1 aaaagttacg gaacgaaaaa caaccaaaac cggacaagtc ggattcccgc ctgcgcggga
       61 atgacggaat cttaagtttc cgtctttgtt ttctgttttc gagggaataa tggggaacaa
      121 gccgtatttc agacggcatt ttcagttcgg ggtataatcc gaatacttgc gaccatctga
      181 atcattggga caaaccatgt gtcaactgct gggcatgaac tgcaatacgc cgaccgatat
      241 tatgttttcc tttgaaggct tccgccgcag gggcggcatt accgaccatc acgccgacgg
      301 tttcggtatc ggctttttcg aaggcaaagg cgtgcgcctg ttccacgacg acaagccgag
      361 cgcaaattcc cccgtcgccg acctcgtgcg tgcctaccaa atcaaatcgg aaaacgtcat
      421 cgcacatatc cgcaaagcat cgcaaggaca aacctcgctg gcgaacaccc atccctttat
      481 gcgcgaaatg tggggcggct actggctgtt tgcccacaac ggacatttga ttgatttttt
      541 ccccgaacag ggcgaatttt tccaccccgt cggcacaacc gattccgaac gcgcgttctg
      601 ccacatcctc aaccgcctgc gcacccgctt tgccgcccgt cccgacgacg acacgctgtt
      661 tgacgcgatt gcggggctga cgcacgaaat ccgcaagttc gggctgttta actttatgct
      721 ttcagacggc attgccctgt ttgcccacgc cagcacgctg ctgcactaca tcgtccgcca
      781 agccccgttc ggcaaggcgc gcctgctcga cgacgacgtg atggtcgatt ttgccgaagt
      841 aaccacgccc tccgaccgcg tcgccgttat cgccaccctg ccactgaccc gcgacgaatc
      901 atggtcccaa cttgccgtgg acgaactggt catgttccgc gaaggcaaca tcgtccgaca
      961 cgaccgtccc gaaaaccccg tctatatgag tgccgaagaa ggtctggaaa tcgcccgcgc
     1021 cgccggcgta tcggtttgac gttcagacgg catcaggaag aaacgctatg aaatgcccgt
     1081 tttgcgccca ccccgacacc cgcgtcgccg attcgcgcct gatggaagaa cgcaacgccg
     1141 tgcgccgccg ccgccactgc cccagctgcg gcaaacgctt cggcacgctc gaaaccgccg
     1201 aactcaaaat gcccgccgtc atcggtccgg acaaaaaacg ttcgcccttt aatgcacaac
     1261 gcctccgcaa cgacctgacc gccgccgccc gaaaatccgc cctgacaccc gaacagatcg
     1321 acgaaaccgt ccgcctgacg gaacacaggc tctacacttc gggtcagcgc gacatcccct
     1381 ccaccgaact tgccgacatg gtgctaaaag agctgcttga gcacaacacg gaagccgccg
     1441 tccgtttcgc cgcactgcac aaacacttcg acaatccggc agactttgcc tcgtggctgg
     1501 cgcaagccgt caaaacagac cgcaaagcct gattccccca acccatattg atacggtatc
     1561 cccatgtttt cggacacaga tatatccatg atggaaaacg ccctcgacct tgccgattta
     1621 gggcgtttct ccacctcgcc caatccgcgc gtcggctgcg ttatcgcaca cggcagccaa
     1681 attgtcgggc agggcttcca cgtcaaagcg ggcgaaccgc acgccgaagt ccacgccctg
     1741 cgtcaggcgg gcgaaatggc acaaggcgcg accgcctttg ttaccctcga accgtgcagc
     1801 cattacgggc gcacaccgcc ctgtgccgaa gcactggtgc gggcgggcgt gtcccgcgtc
     1861 gttgccgcca tgcgcgaccc caacccgctg gttgcaggca aagggcttgc cctgctcgaa
     1921 gcagcaggca tcaagacgga atgcggttta ctcgaacatc aggcaaggga actcaaccga
     1981 ggcttcctgt cgcgcatcga acgccgccgc ccctttgtcc gcctcaaatg cgccgtttcg
     2041 ctggacggca aaaccgccct ttcagacggc agcagctttt ggattaccgg cgaagacgcg
     2101 cgtgccgacg tacaggtttt gcgtgccgaa agctgcgcgg tgctgaccgg catcggcacg
     2161 gtgttggcgg acaatccccg gctcaacgtc cgcgcttttc caactttgcg ccaacccgca
     2221 cgcatcgttt tagacagccg cctgcgcctg cccccgaaca gccatttggt taccgacgga
     2281 caatctccga cctacatcgc cacactcgaa cgcaacgaag acagactgca cccctatcgg
     2341 gaacacgcac acgtccgcat cctgatgccg tctgaaacgg cagacagcaa aatcgacctg
     2401 caccacctga tgcgcctcct tgctgacgaa ggtttcggcg aaatcatggt cgaagcaggc
     2461 tccgaactca catccgcatt tttggcagaa aatctggcag acgaaatcgt cctgtaccgt
     2521 tcgcccaaaa tcctcggcag cggcaaagac ctgttttccc tgctcgaaaa ccgcgccgcc
     2581 ctttccgcac cgcccttgtg gacacccgtt tcaagcgaaa tcctcggaca caacatcaaa
     2641 accgtgttcc gaaaaaacgg caacgccttt taaagggttt gcgccgtttc actatataat
     2701 aacgccgata aaaaacggcc ccgttcagac cgccgcccgc ccgaaaaaac gcaacccgga
     2761 ctgccgcacc ccgccggcag ccgcgacggt ctgaaagccg tcaaattccg atcaagaaag
     2821 gcttcagacg gcacaggcag catcccgccg ccgcccggac atcaaaaatg gacacaaaag
     2881 aaatcctcgg ctacgcggca ggctcgatcg gcagcgcggt tttagccgtc atcatcctgc
     2941 cgctgctgtc gtggtatttc cccgccgacg acatcgggcg catcgtgctg atgcagacgg
     3001 cggcggggct gacggtgtcg gtgttgtgcc tcgggctgga tcaggcatac gtccgcgaat
     3061 actatgccac cgccgacaaa gacaccttgt tcaaaaccct gttcctgccg ccgctgctgt
     3121 ctgccgccgc gatagccgcc ctgctgcttt cccgcccgtc cctgccgtct gaaatcctgt
     3181 tttcactcga cgatgccgcc gccggcatcg ggctggtgct gtttgaactg agcttcctgc
     3241 ccatccgctt tctcttactg gttttgcgta tggaaggacg cgcccttgcc ttttcgtccg
     3301 cgcaactcgt gcccaagctc gccatcctgc tgctgctgcc gctgacggtc gggctgctgc
     3361 actttccagc gaacaccgcc gtcctgaccg ccgtttacgc gctggcaaac cttgccgccg
     3421 ccgccttttt gctgtttcaa aaccgatgcc gtctgaaggc cgtccggcac gcaccgtttt
     3481 cgcccgccgt cctgcaccgg gggctgcgct acggcatacc gatcgcactg agcagcatcg
     3541 cctattgggg gctggcatcc gccgaccgtt tgttcctgaa aaaatatgcc ggcctggaac
     3601 agctcggcgt ttattcgatg ggtatttcgt tcggcggggc ggcattattg ttccaaagca
     3661 tcttttcaac ggtctggaca ccgtatattt tccgcgcaat cgaagaaaac gccccgcccg
     3721 cccgcctctc ggcaacggca gaatccgccg ccgccctgct tgcctccgcc ctctgcctga
     3781 ccggcatttt ctcgcccctt gcctccctcc tgctgccgga aaactacgcc gccgtccggt
     3841 ttatcgtcgt atcgtgtatg ctgccgccgc tgttttgcac gctggcggaa atcagcggca
     3901 tcggtttgaa cgtcgtccgc aaaacgcgcc cgatcgcgct cgccaccttg ggcgcgctgg
     3961 cggcaaacct gctgctgctg gggcttgccg tgccgtccgg cggcgcgcgc ggcgcggcgg
     4021 ttgcctgtgc cgcctcattc tggctgtttt ttgccttcaa gaccgaaagc tcctgccgcc
     4081 tgtggcagcc gctcaaacgc ctgccgcttt atctgcacac attgttctgc ctgacctcct
     4141 cggcggccta cacctgcttc ggcacgccgg caaactatcc cctgtttgcc ggcgtatggg
     4201 cggcatatct ggcaggctgc atcctgcgcc accggaaaga tttgcacaaa ctgtttcatt
     4261 atttgaaaaa acaaggtttc ccattatgaa aatcgttttg accacatcta tggcaggctt
     4321 gggcggcacg ggcacgatat catcgattgc caaatggcgt gcaaaaacga ccccgttcag
     4381 tccgacgaaa tcgtccgccg cttcaggcgc gacatttcct atcggaaaat cgtcaacctg
     4441 attgaaagat tggcaaatga gtaaattctt caaacgcctg tttgacattg ttgcctccgc
     4501 ctcgggactg attttcctct cgccagtatt tttgattttg atatacctca tccgcaagaa
     4561 tctaggttcg cccgtcttct tctttcagga acgccccgga aaggacggaa aaccttttaa
     4621 aatggtcaaa ttccgttcca tgcgcgacgc gcttgattca gacggcattc cgctgcccga
     4681 cggagaacgc ctgacaccgt tcggcaaaaa actgcgtgcc gccagtttgg acgaactgcc
     4741 tgaattatgg aatatcttaa aaggcgagat gagcctggtc ggcccccgcc cgctgctgat
     4801 gcaatatctg ccgctgtacg acaacttcca aaaccgccgc cacgaaatga aacccggcat
     4861 taccggctgg gcgcaggtca acgggcgcaa cgcgctttcg tgggacgaaa aattcgcctg
     4921 cgatgtttgg tatatcgacc acttcagcct gtgcctcgac atcaaaatcc tactgctgac
     4981 ggttaaaaaa gtattaatca aggaagggat ttccgcacag ggcgaagcca ccatgccccc
     5041 tttcacagga aaacgcaaac tcgccgtcgt cggtgcgggc ggacacggaa aagtcgttgc
     5101 cgaccttgcc gccgcactcg gccggtacag ggaaatcgtt tttctggacg accgcgcaca
     5161 aggcagcgtc aacggctttt ccgtcatcgg cacgacgctg ctgcttgaaa acagtttatc
     5221 gcccgaacaa tacgacgtcg ccgtcgccgt cggcaacaac cgcatccgcc gccaaatcgc
     5281 cgaaaaagcc gccgcgctcg gcttcgccct gcccgttctg gttcatccgg acgcgaccgt
     5341 ctcgccttct gcaacagtcg gacaaggcag cgtcgttatg gcgaaagccg tcgtacaggc
     5401 aggcagcgta ttgaaagacg gcgtgattgt gaacactgcc gccaccgtcg atcacgactg
     5461 cctgcttaac gctttcgtcc acatcagccc aggcgcgcac ctgtcgggca acacgcatat
     5521 cggcgaagaa agctggatag gcacgggcgc gtgcagccgc cagcagatcc gtatcggcag
     5581 ccgcgcaacc attggagcgg gcgcagtcgt cgtacgcgac gtttcagacg gcatgaccgt
     5641 cgcgggcaat ccggcaaagc cgctgccgcg caaaaacccc gagacctcga cagcataagc
     5701 gattaaaata cacccccgta cagaccgatt ttgacaacac ctgcggcgcg cgcccgattc
     5761 ttcggaacac gccccccttc agacggcata gggtcggaaa tgccgtctga aaaccgacgg
     5821 acaaaccatc atgctgaaca ctttcctttc cccgtggccc tgcttcaccc aagaagaagc
     5881 cgatgccgtt tccaaagtcc tgctgtccaa caaagtcaac tactggacgg gcaacgaatg
     5941 ccgcgaattt gaaaaagaat ttgccgcctt tgccggcacg cggtacgccg tcgcccttgc
     6001 caacggcacg ctggcactcg atgtcgcgct caaagcaatg ggcataggcg cgggcgacga
     6061 tgtgattgtt acctcgcgca ccttcctcgc ttccgcgtcc tgcattgtga acgcgggcgc
     6121 aaaccccgtg tttgccgatg tggatttgaa cagccaaaac atcagcgcgg aaaccgtcaa
     6181 agccgcgctg acaccgacta ccaaagccgt catcgtcgtc cacctcgccg gtatgcccgc
     6241 cgaaatggac ggcattatgg ctttggcaaa agaacataat ctttgggtaa tcgaagactg
     6301 cgcccaagcg cacggcgcaa aatacaaagg caaatccgtc ggctctatcg gacacgtcgg
     6361 cgcgtggtcg ttctgccaag acaaaatcat gaccaccggc ggcgaaggcg gtatggttac
     6421 gaccaacgac aaaaccctgt gggaaaaaat gtggtcgtac aaagaccacg gcaaaagcta
     6481 cgatgccgtg tacaaccacg aacacgcgcc cggtttccgc tggctgcacg aaagtttcgg
     6541 cacaaactgg cgtatgatgg aaatgcaggc ggtcatcgga cgcatccagc tcaaacgcct
     6601 gcccgaatgg acggcgcgcc gccgagaaaa cgccgccaag ctggcggaaa gtttgggcaa
     6661 attcagcagc atccgcttgg ttgaagtcgc cgactacatc ggacacgcgc aatataagtt
     6721 ctacgccttc gtcaaacccg aacacctcaa agacggctgg acgcgcgacc gcatcgtcgg
     6781 cgaactgaac gcgcgcaaag tcccctgcta tcaaggcagc tgctccgaag tctatttgga
     6841 aaaagccttc gacaacacgc cgtggcgacc gaaagagcgt ttgacaaatg ctgtcgagtt
     6901 gggcgacacc agcctgatgt tcttggtgca cccgacgctg accgacgacg aaattgcgtt
     6961 ttgcaaaaaa cacatcgaag ccgtcttgac cgaagccgca cgataaccct tcagacggca
     7021 tatgccgcct gaaaacacat accgccccac gatatgaatc tggaaactct gatcgccctg
     7081 ccgcgcaaca tcaagaaaat ctgtttcctc atacacgatt ttctgatgat tttcattgcc
     7141 ttttggttca cccaaagcct aaaggccgac tactcggacg aatggttcga ttttgccaac
     7201 tggcagtctt ttttgctgac tgccttgctg accatcacat tatttatccg aatggggctc
     7261 taccgtgccg ttacacgctt catcagcttc cgcatcctct ccaccgcgct ggcaggcagc
     7321 ctcgcctccg ccgtattgtt cttcctcaac acattgattt ttgaagaaag gctgcgcctc
     7381 gccctgccga ttgtctattt cttactgctg tttgtttccg tgaccggctc gcgtatggtt
     7441 ttgcgcggac tgttgtccga acaccccaaa aaacagatga tccccgtcat catttacggc
     7501 gcggggcagg cgggcagaca gcttctcgag gccgtcaaac aaatgcgcga atattccgcc
     7561 gccgcctttg tagacgacga ccccaaactg tggcacaccg tcatctacga ccttgccgtt
     7621 taccagcccg atgccatcgc cttcctcatc gaacgctacg gcgtggaaaa aatcctactc
     7681 gccatccccg gcgcgaccca ggaacaacgc cgccgaatca tcaacaaact ggaagcctat
     7741 ccgtgcgaag tgttgaccat tcccggaatg aaagacctga tggacgggaa aatcagcatc
     7801 ggcacgctca aaaaaatctc tgtgtccgac ctgctcgggc gtgattccgt cgcgcccgac
     7861 aaccgcctga tgagtgccga catcgaaggc aaaaccgtca tggtaaccgg cgcgggcggc
     7921 tccatcggtt cggaactctg ccgccagatt atccgccgcc gccccgaaaa gctgctgctg
     7981 ttcgagttat ccgaattcgc cctgtacgcc gtcgaaaaag aattgcgcga aacctgcatc
     8041 caaaaacgcc tcgacaccga aatcctgccc tttctcggtt cggtgcaaaa ccgcacgctg
     8101 ctcgaacacg tcatgaccgc cttttccgtt gcgaccgtct atcacgccgc cgcctacaaa
     8161 cacgtcccca tggtcgagtt caacaccgtc gaaggcatac gcaacaacat cttcggcaca
     8221 ctcgagtgcg cgcttgccgc cacgacatcg ggcgtggaaa ctttcgtcct catctccacc
     8281 gacaaagccg tccgccccac caacaccatg ggtgccagca aacgcatggc ggaactctgc
     8341 cttcaggcgc tcgccgccga acccggacaa aaaacccgct tcagcatggt gcgtttcggc
     8401 aatgttttag gttcgtccgg ctccgtcgtc cccgcatttg aaaaacaaat tgccgaaggc
     8461 ggcccgatta ccctgaccca ccaagacatt acccgctact tcatgaccat tcccgaagcg
     8521 gcgcaactgg ttatccaagc cggcgcgatg ggcaaaggcg gcgacgtatt cgtcctcgat
     8581 atgggcgaat ccgtcaaaat catcgacctt gcccgccaaa tgattacctt aagcggcctc
     8641 aaacccaaaa cacccgaaca acccgacggc gacatcgaaa tcctcattac cggactgcgt
     8701 cccggcgaaa aactctacga agagctgctc atcggcgaca acgtccgcaa aaccggccat
     8761 ccgcgcatta tgacggctga cgaggcgatg ctgccgtggc acgagctctc tgtcctgctc
     8821 gaccgcatcc gtgcggcctg cgaccgttac gaccagcagg caatccgcac cctgctcatc
     8881 aacgccccga ccggctttac cccaagcgac ggtatctgcg acctgctttg ggtacgagaa
     8941 acacacagaa aaaatgccgt ctgaaccttc agacggcata acgtacaaac caacctacct
     9001 tacacacacg gagtttgaca tgcagttctc agcattcggc gaaaaattca cgcaacacag
     9061 cggcatcctc caactgatgg acgacctcgg cgacgcgctc aaaagcgaca agcccgtcaa
     9121 tatgctcggc ggcggcaatc cggcgcgaat cgccgaagtc aatgccgtgt tcgccgacgt
     9181 attttccaaa ctggcagcag aacatgccgt cgaaaacatc ggcaactact ccaatcccca
     9241 aggcgatgcc gtgctgattg acgcgctgac cgccttcctc aaccgcgaat acagctggaa
     9301 tctgaccgcc gacaatatcg cgctgaccaa cggttcgcaa aacgcgtttt tctatctttt
     9361 caacctcttc ggcggcaaat tcaacctttc agacggcaca tccgcagaaa aagccatttt
     9421 gttgccgctc gcgcccgaat acatcggcta tgccgacgtg catgtcgaag ggcagcactt
     9481 cgtttccgtc aagcccaaaa tcgaaaacgt cgaacacgaa ggcgaagccg gcttcttcaa
     9541 ataccgcgtg gactttgacg cactggaaaa cctgcccgaa ctcaaagcgg gcaaaatcgg
     9601 cgcgatttgc tgttcgcgcc cgaccaaccc gaccggcaat gtgttgaccg acggcgaaat
     9661 ggcgcgtttg gacgctttgg cgcgtgaaca cggcattccg ctgattatcg acaacgccta
     9721 cggaatgccg ttccccaaca tcatttacag cgacgtaacg ctgaattggc acgaaaacat
     9781 catcctctgc ttcagcctgt ccaaagtcgg cctgccgggc gtgcgcaccg gcatcatcgt
     9841 cgccgcgccc gaagtcgtca aagccgtcag cagcctgaac gcgattgtga accttgcccc
     9901 cacgcgcttc ggcgcggcca tcgcaacgcc gctgctggaa agcggcgaga tgaaacggct
     9961 tgccgaccaa gtcatccggc cgttttaccg caatcaggcg caaaccgccg tctcgctgct
    10021 caagcgggag ctgggcgcgt acccgatgaa aatccacaaa cccgaaggcg cgattttcct
    10081 gtggctctgg tttgaaaacc tgcccgtttc ttcgcaaacc ctgtacgaaa tgctcaaagc
    10141 cgaaggcaca ctgattattc cgggcgaaca tttcttcgtc ggcatcgaca cgcaggatta
    10201 cccgcatgcg ggcgagtgca tccgcatgag catcgcgcag gacgctcaaa cgctggaaaa
    10261 aggcattgcc gccatcggta aaaccgtccg aaaactgtac gacaacgttt aaaacgcaaa
    10321 aaatgccgtc tgaaaagttt tcagacggca tttttatctg cattcaatat cgggaaaaat
    10381 gttcccaaac cggtttgcag ttttccggca gctcgggaca cgcgccgagg atgccgccgc
    10441 tgaagtcgtt taagcggtgg aagtcgctgc ccgcgctggc gagcataccg aagcgttctg
    10501 ccaaaagcgc gtagttgagg cggtcgtttt tgcagcagtt tccgctgtgg acttcgatgc
    10561 ctgcgccgcc gaggttttta aattcttcaa acaaattgcg cttggcggtg gcggacaaat
    10621 cgtagcgcat ggggtgggcg atgactgcca tgccgcccgc tccgttgacg gcggagacgc
    10681 agtcttccag cgtcgcccat tcgtggcgga cggcgcagga tttgccgtcg cccaagtatt
    10741 tggtgaacgc ctgctgcttg tttttgacgt gtcccgcttg gatgaggaac tcggcgacgt
    10801 gggtgcggct gaccatttct ttgtttgccg ccagtgccag cgcgccgtcg tatgcgccgc
    10861 cgatgccttt cttttcgagc ttggcggcga tggcttcaag acgtttcaga cggcctttcc
    10921 gcacttgcgc caacaggttt tgcaggtttt cgtcctgctc gtcgaaatcc aaaccgacaa
    10981 cgtgtatggt gcgcccgcgc cacgttacgg agatttccac accgttaatc aggcgcaaac
    11041 cgagcttgtc ggcttcggcg cgcgcttcgg cgatgccgcc ggtgtggtcg tggtcggtca
    11101 acgccagcag cgtgcagccg ttttgatgcg cgaggcgcac gacttcggcg ggggagagca
    11161 taccgtcgga aacggtggaa tggcagtgca ggtctatcat ggggtgttat gtgtgttgtg
    11221 aatgaaggtc gggggttaat attatcggtt ggtgtggata cagcggtgat ttcaacaaac
    11281 aggtgtatgg caaatgcaaa ggaaaagtcc ctatgccgtc attcccgcgc aggcgggaat
    11341 ccagaccttg atttgtcaaa aatatttaag gttaaccgct atttcgaact tccggattcc
    11401 cgcctgcgcg ggaatgacga tatggacgtt ttcagtttta atctactata aaagactgtc
    11461 tgaaaacgtg gttttatagt gaattaaatt taaaccggta cagcgttggc tcgcctt
//
LOCUS       DAWIVB010000044        11432 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWIVB010000044 DAWIVB010000000
VERSION     DAWIVB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.044"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..79)
                     /locus_tag="WIX60_000869"
     CDS             complement(<1..79)
                     /locus_tag="WIX60_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ5305158.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WIX60_000870"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WIX60_000870"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ5305159.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WIX60_000871"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WIX60_000871"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ5305160.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WIX60_000872"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WIX60_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ5305161.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVIGKGRKQRQVPLVGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /locus_tag="WIX60_000873"
     CDS             4804..5868
                     /locus_tag="WIX60_000873"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ5305162.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            5888..6865
                     /locus_tag="WIX60_000874"
     CDS             5888..6865
                     /locus_tag="WIX60_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305163.1"
                     /translation="MPSEPPFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTTCCCLSLTAALILTACSSGGGGSGGGGVTADIGTGLADALTAPLDHKDKGLQ
                     SLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLIT
                     LESGEFQVYKQSHSALTALQTEQEQDPEHSEKMVAKRRFKIGDIAGEHTSFDKLPKDV
                     MATYRGTAFGSDDAGGKLTYTIDFAAKQGHGKIEHLKSPELNVDLAVAYIKPDEKHHA
                     VISGSVLYNQDEKGSYSLGIFGEKAQEVAGSAEVKTANGIHHIGLAAKQ"
     gene            7004..7681
                     /gene="tsaB"
                     /locus_tag="WIX60_000875"
     CDS             7004..7681
                     /gene="tsaB"
                     /locus_tag="WIX60_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ5305164.1"
                     /translation="MQADFNRPVLAIDTGTSHLSLALRADGETRLFHQEVGSRQSELI
                     LPEIRTLFRDAGITAADLGAVVYAQGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRCRLSDYQVGRAADIRLPEGCAFSDG
                     IGNAFALENRPPFTGKPDMPTAADFLNLAEEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRAQT"
     gene            7678..8118
                     /gene="rimI"
                     /locus_tag="WIX60_000876"
     CDS             7678..8118
                     /gene="rimI"
                     /locus_tag="WIX60_000876"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ5305165.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRIAAFIVWQNLPDESELHLIATALEYRRRGVASALLEYWFANLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8112..8852
                     /locus_tag="WIX60_000877"
     CDS             8112..8852
                     /locus_tag="WIX60_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ5305166.1"
                     /translation="MLSARYLHLHEALGLGPMWLKRGAVVLPSATLPESPTQIRPQKQ
                     TVLSIPQRPPEQHTGQARLKTMKVLETAAVPTRKPVPETETPLSGVSDGIAPVPAASG
                     ITKLAVVSLCPPTEDMVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNP
                     MPSEQAIANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPA
                     RLLRQPELKARNWQVLKQLKHALQAGGS"
     gene            8914..9555
                     /gene="pyrE"
                     /locus_tag="WIX60_000878"
     CDS             8914..9555
                     /gene="pyrE"
                     /locus_tag="WIX60_000878"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ5305167.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRQSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVIPIANLNDLFTLLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9629..10063
                     /locus_tag="WIX60_000879"
     CDS             9629..10063
                     /locus_tag="WIX60_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305168.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDAKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10060..11370
                     /gene="argA"
                     /locus_tag="WIX60_000880"
     CDS             10060..11370
                     /gene="argA"
                     /locus_tag="WIX60_000880"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ5305169.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYGGKTLHLDMLQTAASAAVSLQAEKLVYLTLSDGIARPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
BASE COUNT         2587 a         3597 c         2997 g         2251 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccatacagc cggtttcata cacatctccc attaaagcca aacattatac agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat cctgcctcca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcgt gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgacc gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aactggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcaa tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgcgccatcc gtcctgattt gtgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgcc ggacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat ggtcggcacg gtacaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgt accgccaaca
     1501 accgcgtcgt caacttcacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttctcacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcaaca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat gttcggcacg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg cgatgccgac atcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgctt tctttaggca ggttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcggc gagtttgtag ccgttgccct ttttggtgat gacgtgcagc
     2761 aactgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt gtagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggaggtgg aggaatgccc cacgccgaac gcgtcgtact cggactcgca acgtttcgga
     3301 aaacccgcca aaccgccata ttggcgcatg gtgtgcatct ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgtcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca aattgctggc gaaatgcccg
     3481 ccggtctgcc cgacagattc cagcagaaag gtgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggtcaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gcttggtata aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aataggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccgaccagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 ataacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagt tcgaacaggg cgtggtcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt ccaccagcca ggcgcaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgccgc
     4441 gacaagtcct gcggcacgcc gcctgcatct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgct ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggca gtcgaagaat ttccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt
     5101 tcctccgtga tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcttgcgg cgtatccgtc
     5221 gaaggcgaaa tcggcgtgtt gggcaacctc gaaactggcg aagccggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg
     5341 cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc
     5401 gcgtacaaat tcacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gttccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgacaccgac
     5641 ctgcgtcttg cttccaccgg cgcagtacgc cgctaccttg ccgaaaaccc gtccgacttt
     5701 gacccgcgca aatacctgag caaaaccatt gaagcgatga aacaaatctg cctcgaccgt
     5761 tatcttgcgt tcggctgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaagcc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgcttgt
     5881 aaacaaaatg ccgtctgaac cgccgttcgg acggcatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgtttata tattatctat
     6001 tctgcgtatg actaggagca aacctgtgaa ccgaactacc tgctgttgcc tttctctgac
     6061 cgccgccctg attctgaccg cctgcagcag cggaggaggc ggaagcggag gcggcggtgt
     6121 caccgccgac atcggcacgg ggcttgccga tgcactaacc gcaccgctcg accataaaga
     6181 caaaggtttg cagtctttaa cgctggatca gtccgtcagg aaaaacgaga aactgaagct
     6241 ggcggcacaa ggtgcggaaa aaacttatgg aaacggcgac agccttaata cgggcaaatt
     6301 gaagaacgac aaggtcagcc gtttcgactt tatccgtcaa atcgaagtgg acgggcagct
     6361 cattaccttg gagagcggag agttccaagt gtacaaacaa agccattccg ccttaaccgc
     6421 ccttcagacc gagcaagaac aagatccaga gcattccgag aagatggttg cgaaacgccg
     6481 gttcaaaatc ggcgacatag cgggcgaaca tacatctttt gacaagcttc ccaaagacgt
     6541 catggcgaca tatcgcggga cggcgttcgg ttcagacgat gccggcggaa aactgaccta
     6601 tactatagat tttgctgcca aacagggaca cggcaaaatc gaacatttga aatcgccgga
     6661 actcaatgtc gatctggccg tcgcctatat caagccggat gaaaaacacc atgccgtcat
     6721 cagcggttcc gttctttaca accaagacga gaaaggcagt tactccctcg gtatctttgg
     6781 cgaaaaagcc caggaagttg ccggcagcgc ggaagtgaaa accgcaaacg gcatacacca
     6841 tatcggcctt gccgcgaagc aataaccatt gtggaaatgc cgtccgaaca caataattta
     6901 ccgttcggac ggcatttttg cgccataccg ccctaggaca cgacggcacg gcaggcaagc
     6961 cgatttcact atataatgcc gtctgaacca acgagagaat gccatgcaag ctgattttaa
     7021 ccgtcccgtc ctggctatcg ataccggtac ttcccatttg tcgctcgcgc tgcgtgccga
     7081 cggcgaaacc cgtctgttcc atcaggaagt cggcagccgc cagtccgaac tgattctgcc
     7141 ggaaatccgc accctgttcc gtgatgcggg cattaccgcc gccgatttgg gtgcggtcgt
     7201 gtacgcacag ggtcccggcg cgtttaccgg actgcgtatc ggcatcggcg tggcgcaagg
     7261 tttggcaacg ccgtttgata cgcctttaat cggcatcccc acgctcgatg ccgccgcctc
     7321 gctgccgccg ccgcaaagct gcatactcgc tgctgcggac gcgcgtatgg gcgaagtgtt
     7381 ttatgcgtgg ttcgacacgc tgaaccgctg ccgtttgagc gattatcaag tcggtagggc
     7441 ggcagacatc cggctgccgg agggatgcgc cttttcagac ggcatcggca atgcgttcgc
     7501 gctggaaaac cgtccgccct tcacaggcaa acccgatatg ccgaccgccg ccgattttct
     7561 caatctggcg gaagaaggcg gttttcccgc cgtcgctgcg gcacacgccg agctgctcta
     7621 cgtccgcaac aaaatcgccc tgactgccaa agaacaggcc gaacggagag cccaaacatg
     7681 aacatccgcc gcgcctcacc gtccgactgc gccgcactgg ccgcgctcga tgccgtctgc
     7741 aacccgtccg cgtggacgca acgccaattt gaatccgcac tcgtttcgcc gtccgaacag
     7801 gttttccttg cggaaaaaga cggcaggatt gccgccttta tcgtttggca gaacctgccc
     7861 gatgaatccg aactgcacct gattgccacc gctttggaat accgccgccg gggtgtcgcg
     7921 tccgccctgc tcgaatactg gttcgccaac ctgcctgaag gcacgcaacg cctgctgctc
     7981 gaagtccgcg caggcaacac cgccgcacag gcactgtacg cggcgcacgg ctttaccgat
     8041 gcgggcaggc ggaaaaacta ttaccgtaca gccgacggta aaaccgaaga tgccgtctta
     8101 atggagaaaa tatgttaagc gcgcgctacc tccacctgca cgaagccttg ggtttgggcc
     8161 cgatgtggtt gaagcgggga gccgttgtcc tgccgtcagc aacattaccg gaaagcccga
     8221 cacaaatccg cccgcaaaag caaaccgtcc tcagcattcc gcagcgtccg cccgaacagc
     8281 ataccggtca ggcacggctc aaaaccatga aagtgttgga aaccgccgcc gtacctacgc
     8341 gcaaacccgt gcctgaaacc gaaacgcctc tgtccggcgt ttcagacggc atcgcccccg
     8401 ttcccgccgc ttcgggcata accaaacttg ccgtcgtcag cctgtgtccg ccgaccgagg
     8461 atatggttta cgggcagctg ttccacggca aagcgggtgt cctgctcgac aatatactca
     8521 aagccgtagg actggatgcc gcttatgtac acaagaccag ctgggtgaaa accgccgccg
     8581 tcggcaaccc gatgccgtct gaacaggcca tcgcgaatgc gctgggtcaa atcgccaggg
     8641 aactcgacgg ctgccgcgcc ccggccgtcc ttttcctcgg gcaggctttt gtccagcctg
     8701 aacggcaaac gatgattgaa actttgtgcg gcagccgtcc cttcttcatc atcgaccatc
     8761 ccgcccggct tttacgccaa cccgaactta aagcccgaaa ctggcaggtg ttgaaacagt
     8821 tgaaacacgc cttgcaggca ggcggcagtt gaagcaggcc gcacggggcg gtagaatcac
     8881 aactgcatcg caatacctga cagaaagcaa aaaatgaccg atttccgcca agatttcctt
     8941 aaattctccc tcgcccaaaa tgtcttaaaa ttcggcgaat ttaccaccaa ggcaggacgg
     9001 cagtcgcctt atttcttcaa tgccggcctc tttaacgacg gcttgtccac gttgcaactg
     9061 gcaaaattct atgcacaatc catcattgaa agcggcatcc gattcgatat gctgttcggg
     9121 ccggcctaca aaggcattat tttggcggcg gcaaccgcga tgatgctggc ggaaaaaggc
     9181 gtgaacgtcc cgtttgccta caaccgcaaa gaagccaaag accacggcga aggcggcgtg
     9241 ttggtcggcg cgccgctcaa agggcgcgtg ctgattatcg acgacgtgat ttccgccggc
     9301 acatccgtac gcgaatcgat caaactgatt gaagcggagg gcgcaacccc cgccggtgtc
     9361 gccatcgcgc tcgaccgcat ggaaaaaggc accggcgaat tgagcgcggt tcaggaagtg
     9421 gaaaaacaat acggcctgcc cgttattccc attgccaatt tgaacgattt gtttaccctg
     9481 ctgcaaaaca accccgaatt cggacagttc ctcgaacccg tccgagccta ccgtcggcag
     9541 tacggcgtag aataaaaaca aagcatatgc cgtccgaacc gccttacgcc tcagacggca
     9601 tcaaacctga cacacacgag gaaatactat gccagcctgt ttctgccccc actgcaaaac
     9661 ccgtctctgg gtcaaagaaa cccaactcaa tgtcgcccaa ggcttcgtcg tctgccaaaa
     9721 atgcgaagga ctgtttaaag ccaaagacca tctggcaagc acgaaagaac ccatattcaa
     9781 cgatttgccc gaggctgttt cggatgccaa actcgttcac cgtatcggca cgcgcgccat
     9841 cggcaagaaa cagatttccc gtgacgaaat cgccggcatc ctcaacggcg gcacaaccca
     9901 gcccgatatt ccgcccgcaa ccgccgccac ccctgctgcc gcaccgcagg ttaccgtacc
     9961 gcccgccgcg cccgcccgtc aggatgggtt caactggacg attgcaaccc tgtttgccct
    10021 tatcgtcctc attatgcagc tttcctacct cgtcatccta tgatcgtgcc cgacctcttt
    10081 gtcgcccact tccgcgaagc cgccccctac atccgccaaa tgcgcggcaa aacgctggtc
    10141 gccggcatag acgaccgcct gctcgaaggc gataccttaa acaagctcgc cgccgacatc
    10201 gggctgttgt cgcaactggg catcaggctc gtcctcatcc acggcgcgcg ccacttcctc
    10261 gaccgccacg ccgccgcgca aggccgcacg ccgcattatt gccggggctt gcgcgttacc
    10321 gacgaaacct cgctcgaaca ggcgcagcag tttgccggca ccgtccgcag ccgttttgaa
    10381 gccgcattgt gcggcagcgt ttccgggttc gcgcgcgcgc cttccgtccc gctcgtatcg
    10441 ggcaacttcc tgaccgcccg tccgataggt gtgattgacg gaaccgatat ggaatacgcg
    10501 ggcgttatcc gcaaaaccga caccgccgcc ctccgtttcc aactcgacgc gggcaatatc
    10561 gtctggctgc cgccgctcgg acattcctac ggcggcaaga ccttacatct cgatatgctt
    10621 caaaccgccg cctccgccgc cgtctcgctt caggccgaaa aactcgttta cctgaccctt
    10681 tcagacggca ttgcccgccc cgacggcacg ctcgccgaaa ccctctcggc gcaggaagcg
    10741 caatcgctgg cggaacacgc cggcggcgaa acgcgacggc tgatttcgtc cgccgttgcc
    10801 gcgctcgaag gcggcgtgca tcgcgtccaa atcctcaacg gggccgccga cggcagcctg
    10861 ctgcaagaac tcttcacccg caacggcatc ggcacgtcca ttgccaaaga agccttcgtc
    10921 tccatccggc aggcgcacag cggcgacatc ccgcacattg ccgccctcat ccgcccgctg
    10981 gaagaacagg gcatcctgct gcaccgcagc cgcgaatacc tcgaaaacca catttccgaa
    11041 ttttccatcc tcgaacacga cggcaacctg tacggttgcg ccgccctgaa aacctttgcc
    11101 gaagccgatt gcggcgaaat cgcctgcctt gccgtctcgc cgcaggcaca ggacggcggc
    11161 tacggcgaac gcctgcttgc ccacattatc gataaggcgc gcggcatagg cataagcagg
    11221 ctgttcgcac tgtccacaaa taccggcgaa tggtttgccg aacgcggctt tcagacggca
    11281 tcggaagacg agttgcccga aacgcggcgc aaagactacc gcagcaacgg acggaactcg
    11341 catattctgg tacgtcgcct gcaccgctga ccgcaacgga aagccgccgc agaaatgccg
    11401 tctgaacccc gtttcagacg gcatttcccc ga
//
LOCUS       DAWIVB010000045        11310 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWIVB010000045 DAWIVB010000000
VERSION     DAWIVB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.045"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            57..338
                     /locus_tag="WIX60_000881"
     CDS             57..338
                     /locus_tag="WIX60_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ5305170.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(359..1585)
                     /locus_tag="WIX60_000882"
     CDS             complement(359..1585)
                     /locus_tag="WIX60_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ5305171.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1600..2025)
                     /locus_tag="WIX60_000883"
     CDS             complement(1600..2025)
                     /locus_tag="WIX60_000883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305172.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2130..2789
                     /gene="queC"
                     /locus_tag="WIX60_000884"
     CDS             2130..2789
                     /gene="queC"
                     /locus_tag="WIX60_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ5305173.1"
                     /translation="MSNQQALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2817..3335
                     /locus_tag="WIX60_000885"
     CDS             2817..3335
                     /locus_tag="WIX60_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ5305174.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDNYREAVPALKAAWFG
                     DADKIHIDGWQIVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3342..3764
                     /gene="queD"
                     /locus_tag="WIX60_000886"
     CDS             3342..3764
                     /gene="queD"
                     /locus_tag="WIX60_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ5305175.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYGRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4033..4404
                     /locus_tag="WIX60_000887"
     CDS             4033..4404
                     /locus_tag="WIX60_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ5305176.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKQVQTLFSNQGLYNGFLGIGLVWSRFAAPDNAVYGATTLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAMLAAAAVLAV"
     gene            4401..5039
                     /locus_tag="WIX60_000888"
     CDS             4401..5039
                     /locus_tag="WIX60_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ5305177.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMGLSDILGRLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5374..5450)
                     /locus_tag="WIX60_000889"
     tRNA            complement(5374..5450)
                     /locus_tag="WIX60_000889"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5414..5416),aa:Pro,seq:cgg)
     gene            complement(5678..6763)
                     /gene="nagZ"
                     /locus_tag="WIX60_000890"
     CDS             complement(5678..6763)
                     /gene="nagZ"
                     /locus_tag="WIX60_000890"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ5305178.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHRNPEAVARLALAL
                     QKGLTKGGMKSCGKHFPGHGFVEGDSHLVLPEDWRSLSELETADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPTLAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(6820..8340)
                     /locus_tag="WIX60_000891"
     CDS             complement(6820..8340)
                     /locus_tag="WIX60_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ5305179.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRTKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8542..10041)
                     /locus_tag="WIX60_000892"
     CDS             complement(8542..10041)
                     /locus_tag="WIX60_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ5305180.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAENDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            complement(10179..10808)
                     /gene="nth"
                     /locus_tag="WIX60_000893"
     CDS             complement(10179..10808)
                     /gene="nth"
                     /locus_tag="WIX60_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ5305181.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(10854..11267)
                     /locus_tag="WIX60_000894"
     CDS             complement(10854..11267)
                     /locus_tag="WIX60_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ5305182.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2648 a         3017 c         3162 g         2483 t
ORIGIN      
        1 gttttttcga tacggaaccg gatagaggaa aaatcgaaca ttgcgcctgc cttgctatga
       61 ttcacgaaga aatctccgcc atgcctatgg gctatgaaac cttaatcggc gatatgggca
      121 gcgcactgtc aggcggacaa aaacaacgca tcgtattggc gcgggcctta tattgcgaac
      181 cgaaaatcct atttttagat gaagcgacca gccatttgga tattgccaat gaaaaagcag
      241 tcaatgcaaa cttgaatggc ttgtctatca taaaaattat ggcggcacac agaaaggaaa
      301 cggtggaatc agcagatagg aaaatgtctt taggataaaa atacagtttc aaaaatactc
      361 aagattgctg ctgttttttc gcctgagcgt caaactctgc cagcgtcatg ttcaaagtct
      421 gcaaacacgg catcattacc gcatcgacag cttggttcac atgatccctt tccacaggca
      481 acggacggta aacgaagagc ttgaagagtt cgttcaactc aatcgaatcc gcccccgttt
      541 tcaacaccca accctgtctg ccggaataga tgtagccgtg ccgcgccagc ttttccaaaa
      601 gctcgcccaa ctcgtcgtag cccatattga tatgccgtct gaactcctga acaggcaagg
      661 ctttgccttc tttttgcgcc gcatccagaa gcagcaggat tttcaacacg tcgtcaaacc
      721 gtccgcgcga gtcgaagccc ctgcggaacg cttctccctg ccagtaggag agtgaagaag
      781 tcagcaccgc gccgcccaag accagcgtcc acaacaggtt cagccacaac aggaaaaacg
      841 gcacggcggc aaacgcgccg taaatcgagc ggtagccgtc gaaattgccc atataccaag
      901 taaagaggga acgcgcggtt tccagacaga acgctgttgc caaagccccg acaaacgcct
      961 gccgcgcggg aacgaagcgg tttggcacga agcggtacag cccccacagc aaaagcgtca
     1021 tgaaggtcag cgtcgccgcc gttcgcaacg cgcccgacca ctgcggcgca cctgaggcaa
     1081 gcgcggcatc ctgtaccgag ccgaccataa aggaaatgcc cacgcccaaa gacagcggcc
     1141 cgaacgtcag taaagcccaa tagacgagaa actgcatcat ccacggacgc tgggaattga
     1201 cccgccagat gcggttgaac gtattgtcta tcgtccgaat cagcatcagc gaggtaacga
     1261 ccagcatcac gctgccgatt gccgtcagcc ggttcgcctg ctcgcggaac gcattgatat
     1321 agtcgaacac catgtccgcg ccctgcggca caatggtttg gttgacgaag gagacgaacg
     1381 aatccgacca gcggtcgaac acggggaaaa tcgaagcgac cgccaccatc acggtcagca
     1441 cggggacgag tgccagcagc gtcgtaaacg tcatgcttgc cgccgcctgc ggtacgcgtt
     1501 cttcatcaaa gcggcggacg acgaaccatg caaacgcaca gattttattg tctgccaaac
     1561 cttgcaaacg ttgtaaaaag gtcataattt cttgcccggt cagtaagttg ggcattgatg
     1621 cccgatgtta tagccaattt tgccgtcagg aacaaatgcc tgaactgcgg ctgtttcaga
     1681 cggcatcgga acaactgtta tgccgtctga agaccgaacc attttaacgg aatccgccca
     1741 tgaacccaaa tccccctcaa aatcctcgtc ctctactatt tccaaaacgg cagcacccgc
     1801 aatcccgccc gccgaatcgc tcgcggcatc gacagcgttg aaggttgcga agccgtattg
     1861 cgcaccgtcc ccaaagtctc cgccgtctgc gaagccgtca aaaaagatat tcccgacagc
     1921 ggctcccgtc ctgaccgccg aagaaaacaa tatcgccttc gcacaaagca aacgcttggc
     1981 ggaactcgcc gtcaagtcgg cataagccgc gtgttcagac ggcatggcgt tcagatgccg
     2041 tctgaacacg tttgcctgta taatccgcat ctttactgtc caacttcgcg gttcgcaaac
     2101 ctcccgcgtt accaaaacta ggattcgata tgtcaaacca acaagccttg gtcatctttt
     2161 cgggcggtca ggattcgacc acctgcctga ttcaggcaat ccaaacctac gggcgcgaaa
     2221 acgtccaagc cattactttc caatacgggc aacgccatgc cgtcgagctg gaacgtgccc
     2281 gctggattgc gcaggatttg ggcgtcaaac aaaccgtact cgacttgagc ctgatgcggc
     2341 agattacgca caatgccctg atggacgaca ccgccgccat cgaaactgcc gaaaacggcg
     2401 ttccgaatac ctttgtagac ggccgcaacg cgcttttcct gctctatgcc gcgatttacg
     2461 ccaaagggca ggggatacgg cacatcatcg cgggcgtgtg cgaaaccgac ttctccggct
     2521 atcccgactg ccgcgacgtg tttgtcaaat cgatgaacgt tacccttaat ttggcgatgg
     2581 actatgattt tcaaatccac acgccgctga tgtatctgac caaggcgcaa acgtgggcgt
     2641 tggcggacga aatgggcgtg ctggactata tccgcgagca aacccacacc tgctataacg
     2701 gcatcgtcgg cggctgccgc gaatgcccga gctgtatctt gcgcgaacgc gggctggcgg
     2761 aatatctgga aagtaaaaag gccgtctgaa cacgcgcaaa ccataaggaa tacgatatgc
     2821 ccaagctcca tatgttttac ctcggcggca atgccggcag gtcgaatatc gaagtgcacg
     2881 acatccaatt tgccgtgtgc gacaactacc gcgaggccgt ccccgcgctc aaagccgcgt
     2941 ggttcggcga tgcggacaaa atccacatcg acggctggca gattgtcgaa tgggcggacg
     3001 gttacgacat cgccgtatcc gaaacgccca aaacgaaaat gccgtctgaa cacgccccgc
     3061 gcctgtattt cgccaatgtc ggcggttatc gcgcgggtca gcttgccgag gcacacgctt
     3121 tcgggctgtt cgccgccgcc acgcctgccg aagccaaaca aaaagccctg caaaccctgt
     3181 tgaccgacag ctatgttcag cagcataaag acaacttaaa agacgtggac aacctgcttg
     3241 cgctcgaccg catcggcaat ttccatatcc gcctgacccc gaatccgcac ggcaaacccg
     3301 ccgaaatcgg ctttcaaggc tatttgccca tttgagaacc catgaaaatc accaaaatct
     3361 tcaccttcga ctcctcgcat atgctcgacg ggcatgacgg caaatgccaa aacctgcacg
     3421 gacataccta caaactcgaa atcaccgttt cagacggcat tatcaaaggc ggcgcgaaag
     3481 acggtatggt gatggacttt accgacttga aagccattgt caaacaacac attaccgacc
     3541 ccttcgacca cgccttcatc taccacggcg gcaacagccg cgaatgccaa atcgccgcgc
     3601 ttttggaggg ctggaacatg aaaaccctgc gcctgccctg ccgcaccact gccgaaaata
     3661 tggcggtcga aatgtacggc cgtctgaaaa acgcggggct gaacgtgtgc cgcgtgaaat
     3721 tgtgggaaac gccgacatcg tgtgcggagt atgaagggga gtagggaata tcttgaacgt
     3781 atcgatatag taaattccaa taagacatgc ccaaccgcgt cattcccgcg caggcgggaa
     3841 tccagacctt gatttatcag gaatatttaa aaattgcagc aattccaact ctctggattc
     3901 ccgcctgcgc ggaaaggacg gtttagagcg tccttatttg aatttaccgt aaaacggttt
     3961 tttctcctgt acggattccc cgttttttca gacgaccttc catatcaaat acacccatta
     4021 aaaggaatac ccatgaaact cctcttcatc ctcctagtcc tcttcgtcgc cgtcgaacat
     4081 ttctacatcg cctggcttga aatgacacag attcccagcg aaaaagcggc ggaaatattc
     4141 aagctgcctt atgaatttat ggaacaaaag caagtgcaga ccttgttcag taatcaaggg
     4201 ctgtataacg gctttctcgg catcgggctg gtgtggtcgc ggtttgccgc gccggacaac
     4261 gccgtttacg gcgcgacgac tctgtttctc ggtttcgtat tgattgccgc cgcgtggggc
     4321 gcgttttcgt ccggcaacaa aggcatactc gtcaaacaag gactgcccgc gatgctggcg
     4381 gcggcagcgg tgttggcggt atgaaaaaaa tcaatgttgc ccccgaaaat ccgcaatacc
     4441 gtatcgtcga aattttcgag agcctgcaag gcgaaggctg gaacacgggc atgcccgccg
     4501 ttttcgtccg cttgggcaaa tgcaatctgg cgtgcggctg gtgtgatacc gattatttga
     4561 cattcggtat gatgggcttg tccgatatct taggccgtct gaaaacctac gccgcccgca
     4621 acatcatcat caccggcggc gagccgacca tacagccgca tctcgatatg ctgctggaca
     4681 cgctcaaggc ggaaggctat ttcctctgtc tcgaaaccaa cggactcaat cccgcgccgc
     4741 cgcaaatcga ctacgtcgcc accagcccca aagcctgcta cgccgccaaa tatgaaaata
     4801 gctgtatcga aacagccgac gaagtgcgga ttgttgccga tggtgatgtc cttgcgttct
     4861 gcgaaaacat ggaacgcaaa atccgcgcac atcattacta cctttcgccc tgtgagcaag
     4921 acggtgcgat gaacatctac gacaccatcc gccaaatcgg tattttaaac agtcgccccg
     4981 acgcatccgt gcattggcag ttgagcgtgc agacgcacaa atgggcggga atagagtagt
     5041 ttaagcagtg taactcaaag ggacggcgta cggttttacc gatgtttgac atacggggaa
     5101 agtgtgccgc ttctgcgtgg aaatgccggc atttccaccg cccaatcagg acggagcctt
     5161 actgaataag atgctgccgt tgggtacaag ctcggcttcc taaattccga tggtcttttg
     5221 aaccttgccg atactctgtg ccagtgcgcg caaatggcag ggttagggaa aacgaaatgc
     5281 cgtctgaaac agcattctgt ttcagacggc atttttctgt tgccgccaaa aggaaaaacc
     5341 gcctcggcaa tggatgccga ggcggtttga atatggtcgg aatgagagga ttcgaacctc
     5401 cgcccccttc gtcccgaacg aagtgcgcta ccgggctgcg ctacattccg aattaagtaa
     5461 ggcgtgatta tagcgcaaaa agtgcggcgt gcctataccg ttttgccttt ttgccgcgtg
     5521 tcgggcggat ttaaaacgtt gtgtttgaat acagtgttga taatcatcat tatctttaag
     5581 taattcaata agataacttt ctacctgacc gaaaaaatca ttgcctttcc ctgacaaacg
     5641 gttgatgaaa tcggcagatt gttgaaacgc agccggttta aaaggcttcg ccgactttca
     5701 cgccgcccgc cgtgtcctgc ggcgaggcaa ggccggcaac aaaggcttgc gccgcttgga
     5761 aatccgccgt ctgcatcacg gcttgcgcgg cggcactgcc gagcgtgttg gccatatatt
     5821 gccaacgttg cgccaaagtg ggattgtcag gaatgcggaa atcttcgcgc agttcatcca
     5881 caaggtcggg acggttgcag acgaggacga tgtcgcaacc tgcctcaaag gaaatgcggg
     5941 cgcgttcttt gatgccgcct gccccgcacg cgccctccat agtcaaatcg tccgagaaaa
     6001 tcacgccttt gaacccgatg tcgcggcgca aaatttgttt gagccagatt tcggaaaacc
     6061 ctgcgggctt tgtgtccact tgtggataaa cgacgtgggc gggcataacc gccgccatac
     6121 cttcgcggct cataatgcgg aagggggcga ggtcggcggt ttcgagttcg gacaggctgc
     6181 gccagtcttc cggcaagacc agatggctgt ctccttcgac aaatccgtgt ccgggaaaat
     6241 gtttgccgca ggatttcata ccgccttttg tcaaaccttt ttgaagggcg agggcgaggc
     6301 gggcgaccgc ttcgggattg cggtggaaac tgcggttgcc gatgacgggg cagtttcccc
     6361 agtccaaatc taagacgggc gtgaaggaca aatcgatgcc gcaggcggaa agctcggttg
     6421 ccaaaacccg gccgacttgt ccggcggcgg tttcggcggc ggacgcgccg tctttgtccc
     6481 aaatctcgcc gagcgtactc attgcgggca ggcgggtgaa gccttcgatg aaacgttgca
     6541 ccctgccgcc ttcgtgatcg acggcgataa tgagttcggg tgtgcgcagg gctttgattt
     6601 cggcggtgag tgttttgagt tgttcgatgt tttggaagtt gcggcggaag aggatgatgc
     6661 cgcctacggc gggatcgagc aggcgttgct tttcctcttc ggtcaggcgg aaggcggcaa
     6721 tgtctgccat gacggggccg cgcggaatat gggggacggt cattgcggtt tgctccaaaa
     6781 agcttcagac ggcatatgcc gtctgaacag ggaaaggggt caggcgttgg cgcgtttttt
     6841 atctttcaac agaaaaatca gcaccgccaa tacaatgcct gtcgtgccaa agcccaacag
     6901 cgcggatttt gtcagaccca atgcgaggta gcccgatgcg gcggcggcgg caacggttaa
     6961 ggcgtaaggc agttgcgagg taacgtggtc gatgtggttg cagcgcgcgc cggtggacga
     7021 caggatggtc gtgtcggaaa tgggcgagca gtggtcgccg cataccgccc ccgccattac
     7081 tgcggacata cacgggataa tcagcgcggg ttcgactttg accgccatgg cggcggcaat
     7141 cggcagcata atgccgaacg tcccccagct tgtgcctgtg gcaaacgcca tcacgctggc
     7201 gagcaggaag aggatgacgg gcaggaagcc gggatggatg ttgcccgcaa ccagtgtgga
     7261 gaggtaatcg ccggtgtgca tttcgccgac aaccgtactg atgagccaag cgaggattaa
     7321 aatggcgatt gcgccgaaca tagatttcgc accctgccaa acggctttgg gatagtcggc
     7381 ggttttaatc gtgccgagcg tgcagagaac gacggcaagg acgccgcaag tgccgccgaa
     7441 taccagcgaa gtgtttacgt ccgtgttttc aaatgccccc aaaatgctga aggtttcgct
     7501 tgcctgcgcg ccggtgtaga tcatggcgga aaccgttgag gcgattaagg ccaaaacggg
     7561 aataatcagt gcgtaaacac gacctttggt agcgtctgaa acggcagttt catcgtgggc
     7621 ttcgttcaac gcggcttgtt cgaaacgtgc catcgagccg atgtcgaagg aaaaccatgc
     7681 gacgacgaac accataatca gggcaaacag tgcgtaatag ttcatcaggc tcatggcgac
     7741 aaacgtcccc atcggcgtgt attcggtgat tttgtaggta acgagcagtc cggcaagcgt
     7801 ggcgataatc gacgcgcccc agcttgaaac gggcatcagc acgcacatag gagcggcagt
     7861 ggagtcgagg atgtaggcga gtttggtgcg ggaaacttta aacttgtcgg taacggggcg
     7921 ggcaatcgca ccgacggcga gactgtggaa atagtcgtcg ataaaggtta cgaacacgag
     7981 gcaggcggtc agcattttcg cgccgcgccg gtttttaatg tgccgttttg cccagtcggc
     8041 aaacgcctga ttgctgccgg agtaggtcag cagggaagta aaaataccca aaagtatcag
     8101 gaaaaccaag atttttggtt tgcccagcga ccaatcgccg tctgaccaag ccaagccgac
     8161 gaccatgtct ttcaggtgtg tcagaccgtc gacggggttg ccgccgacca aaaaggcaac
     8221 gccgaccaga ataccgatgc ctaaagacag cagtacgcgg cgggtaatga cggcaagtgc
     8281 cagtgccaaa aagggtggca caaccgagaa aaatgaatgt gaatagtcga tcagctgcat
     8341 ggttatgggg gtgttaagcg tccggatggg agcgtatctg tccgcctccg gtttgggttt
     8401 tgttggcaaa atgggcggaa atattttttg tcgtaaaaaa tatttgttta aaatcaacca
     8461 actgattttt gtaaaatgcc cgttaatcgg tattgacggg cattttatca tttaaaaaat
     8521 attttggtta aattatgtgt gttattgcag gtttaatgcg ataaacagcg tgttgccacg
     8581 gcgcatgatc agcaggggga cgtttttgcc tgccttgtcc atagctttgc ggaaaccggc
     8641 ttcgtcattg acggggactt gcccgacggc aagaatttcg tcgccgcgcc tcaagcctgc
     8701 gcgttctgcc gcgtcggaaa cccgtacgac gacgaggtgt ccgccgctgc tgtcggtatg
     8761 tgtctgaagg gtaatgcctg cggattcgac cgagaacgta ccggattgct gttcggtgta
     8821 gggggcttca tctgttttgg atgatgcgcc gatatgctcg gcggcgttgc ccagcttgac
     8881 tttgattgtg atttcttcgc ctttgcgcca tacgccgagg ctgacttctt ttcccggcgt
     8941 aatggcgccg accataacgg gaaggtcgcc ggaagaacgt atttctccgc cgtcgaggct
     9001 gaggacgatg tcgcccgcct gcaggccggc acgttctgcg gggctgccgg gcaggatttt
     9061 ggcaatcagt gcgccgccgg ctttgtccaa accgaacgat tgtgccaaac cgtaggatac
     9121 ttcttgaata atcacgccca gttgtccgcg ttggactttg ccggtgtttt tcagctgttc
     9181 ggcgacattc atggcaacgt caatcgggat ggcgaaggaa atgcccatga atccgccgct
     9241 gcggctgtat atttgcgagt tgatgccgac gacctgtcct tttaagttga acagcgggcc
     9301 gccggagttg cccggattga tggcaacgtc ggtttggatg aagggtgtgt agctttcgtt
     9361 gggcaggctt ctgcctttgg cggacacgat gccggcggtc acgctgttgt cgaagccgaa
     9421 gggcgcgccg atggcggcga cccattcgcc cggtttcaaa tctttgggat tgccgatttt
     9481 gacgacgggc agctcttccg ttgcgtcgat tttcagaagg gcgacatcgg attggacatc
     9541 cgaaccgatg agtttggcgg tatattcgcg cttgtcgttg agcaggactt tgatactgcc
     9601 catgccggta acgacgtggg tattggtcag gatgtagccg tctttgctga tgatgaagcc
     9661 cgaaccgaag ttcaatccgc cgtcatctgc ttcttcttgg gggatttcgg gcatgttcgg
     9721 gacgaggcgt ttgaaaaatt cgtagaacgg gtcgctgtcg gcaagcgggt cggaatcgtt
     9781 ttcggcattg ctgctgccgt tttgggtgcg cggggcgggg gctgcctgaa tattgacgac
     9841 tgccgggcct tcgctttgaa ccagttggac aaagtcgggc agcagcatac tgacgctgcc
     9901 gtcgtctttg gtgtgtttga tgcgttctac gaaggatgct tcttttttgt ccgcaccgaa
     9961 aaagctgccg gctttgtcgc agcctgccag cgaggcggca cacagtgctg ccaaagcgag
    10021 gtattggtat tttttgaaca cgttttgtcc tttgtcggat gccggtaccg gctttaatgc
    10081 cgtctgaagc gcattttgtc ggcttcagac ggcataggtt gaaattctac aacgtccgtc
    10141 cgaattttca agcgtttcat tttgaagggc ggcggcggtc aggctttggc gggatattcg
    10201 cacaaatcgt tgatgatgca ggtttggcat tgcggtttga gtgccttgca ggtgtagcgt
    10261 ccgtgcaaaa tcagccagtg gtgcgcgtcc atcagaaatt ctttaggaat gaagcgcatc
    10321 agtttgtctt cgacttcgcg cacatctttc ccgggggcga ttttggttcg gttggacacg
    10381 cggaaaatat gcgtatcgac cgccataacg gggtgtccga acgctgtgtt caataccacg
    10441 tttgccgttt tgcgccccac gcccggcaac gattccaaag cctcgcggtc ttccggcact
    10501 tcgccattgt atttttccag caggatgcgg caggtttgca tgatgtgctt ggacttggtt
    10561 ttatacagcc cgatggtttt cgtgtattcc atcacgccgt ccaaacccaa atccagcatc
    10621 gcctgcggcg tatcggcaac cggaaacagc ttcgccgtcg ctttgtttac gccgacgtcg
    10681 gttgcctgtg ccgacagtaa aacggcgatt aaaagctcga aaggggagtt gaagttgagt
    10741 tctgtggtcg gatgggggtt ggcggcgcgg aagcgttcga agatttcttg gcggatgtgt
    10801 ctgttcattt ttttatacgg tgggtttgtg tgttcggcat tataacgtat ggttcaggcg
    10861 gcgtaatatt gcattcccca cagaatgaag gcgtaacgcg ccgttttgcc gataaccagc
    10921 atcagcccgc ttgtccacgg attcaaccgc agccagccgg cggcaagcgg cagtgcgtcg
    10981 ccgacgacgg gcagccaggc aaacgcaagc agccaaatac cgaaacgccg catcagattc
    11041 agtgtttttt cagacggcat ttttcgggag ggcagcaaac gccccatcca ataggaaacc
    11101 atactgccca atccgttggc aaggccggcg cacagcaacg cgccgtatgc gtgttcggga
    11161 aagcggtgga cgaacagggc aaaggcggct tcggatgtgc cgggcaggag ggtggcggaa
    11221 gtgaaggcgg aaaaagcgag cgcggcgtag gcgtaggcgg gcatcattgc aaacagtctc
    11281 aaacaggcaa aaatcggcga cggattgtac
//
LOCUS       DAWIVB010000046        11303 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWIVB010000046 DAWIVB010000000
VERSION     DAWIVB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.046"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(80..1126)
                     /gene="recA"
                     /locus_tag="WIX60_000895"
     CDS             complement(80..1126)
                     /gene="recA"
                     /locus_tag="WIX60_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ5305183.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1380..2144
                     /locus_tag="WIX60_000896"
     CDS             1380..2144
                     /locus_tag="WIX60_000896"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ5305184.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2164..4179
                     /gene="rep"
                     /locus_tag="WIX60_000897"
     CDS             2164..4179
                     /gene="rep"
                     /locus_tag="WIX60_000897"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ5305185.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYQLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQTFMDMFVSYLAKAETSEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4247..5305)
                     /gene="dinB"
                     /locus_tag="WIX60_000898"
     CDS             complement(4247..5305)
                     /gene="dinB"
                     /locus_tag="WIX60_000898"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ5305186.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYAGDVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVSPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(5449..8538)
                     /locus_tag="WIX60_000899"
                     /pseudo
     CDS             complement(5449..8538)
                     /locus_tag="WIX60_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            8812..9591
                     /locus_tag="WIX60_000900"
     CDS             8812..9591
                     /locus_tag="WIX60_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ5305187.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGNTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(9802..11214)
                     /locus_tag="WIX60_000901"
     CDS             complement(9802..11214)
                     /locus_tag="WIX60_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ5305188.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEISALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSDCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
BASE COUNT         2756 a         3170 c         2733 g         2644 t
ORIGIN      
        1 aactcaaatc aagccgttcg gaggcagctc aaaaaacggt acttcgcagc agaagtaccg
       61 tttatcggga tttcaggttt tattcttcgg ggcgttcgcc gtcggtttcg tcctgcgtcc
      121 cttcggtgat gtgcatttct atgccgttga gggcgcggat ttttgcgtcg atttcgtcgg
      181 caatttcggg attttccttc agccagacgc ggacgttgtc tttgccctga ccgattttcg
      241 cgccgttgta gctgtaccac gcgccggatt tgttgatgat gtcgtttttc acgccgatgt
      301 cgatcaattc gccttcccaa ctgatgcctt ctccgtagag gatgtcaaac tctgcctgac
      361 ggaacggcgg cgcgactttg tttttgatga ctttgacgcg ggtttcgttg cccaatactt
      421 cttcaccttt tttgatggat ccggtgcggc ggatgtcgag gcggacggaa gaatagaatt
      481 tcagcgcgtt gccgccggtc gtggtctcgg ggctgccgaa cattacgccg atcttcatcc
      541 ggatttggtt gatgaacaca accagcgtgt tggttttttt gatgtgtccg gtcagtttgc
      601 gcaaagcctg gctcatcagg cgcgcctgca gtccgacatg gctgtccccc atatcgcctt
      661 cgatttcggc tttggggacg agtgcggcta cggaatcgac gactaccata tctatgccgc
      721 ccgaacggac gagtgtgtcg cagatttcca aagcctgttc gccggtatcg ggctgggaca
      781 ggtaaagctc ttcgactttt acgccgagtt tgcgggcgta aacgggatcg aaggcgtgtt
      841 cggcatcgac aaaggcgcac acgccgccgt ttttctggca ttgggcgacg gcttcgaggc
      901 agagggtggt tttgccggag gattctgggc cgaagatttc gacgatgcgc ccgcgcggca
      961 gaccgccgac tccgagggcg aggtctaatc cgagcgatcc ggtggaaatg acttcgaggt
     1021 tttcttcctg ctggctgccg tccattttca tgatggcgcc tttgccgaaa cttttttcga
     1081 tttgcgccag tgcggcggca agtgctttgc ttttgtcgtc tgacattggg gttactccgg
     1141 aacaaatgcg gtatgtggga tgcggcgcaa cacgggctgc ggcgcgggat gtgtatcgtt
     1201 ttcccgatgt gcgggctatc ggtaatgctg cttcacgagg ttgccattat cgcatatttc
     1261 cttgcttgcc gatatgcgcc aggacgcggc ggcttgtgcc ggaatggaat ctggatgccg
     1321 tctaaaaggc ggccggcttt gttataatgg cggctgtttt ttctgtgtgt gcctgtttta
     1381 tgtgttcctg ccttgttgtc aaaaataccg ttatcggaag cggacgcacc aaaatcgccg
     1441 tgccgcttgt cgcccgcgat gccgccgaac tttccgccgt acttgagcaa atcaaaaata
     1501 tgcccttcga tattgcggag ttccgcgccg actttttgga atgcgcgggc agtatcggcg
     1561 aaatattgca ccacacgcag accgtccgcg acgcgctgcc cgacaagccg ctgctgttta
     1621 cgttcagacg gcatggcgaa ggcggctcgt tcccgtgttc ggacgattat tattttgaac
     1681 tgctcgacgc gctgatcgaa agccgcctgc ccgacatcat cgacatcgag ctgttttccg
     1741 gcgaaaccgc cgtccggtgc gccgtggcaa atgctcaaaa aaacggcatc gccgccctgc
     1801 tctgcaatca tgagtttcac cgcacgccgc cgcaagaaga aatcgtatgc cgtctgaaac
     1861 agatggagga ctgcggcgcg gacatctgca aaattgcggt gatgccgcaa agcgcggaag
     1921 atgtgctgac tttgctttcc gccacgctca aagcgaaaga gcttgccgcc aaaccgattg
     1981 ttacgatgtc gatggggcag acgggggcgg tcagccggct tgccggacag gtgttcggct
     2041 caagcatcac gttcggttcg ggaacgcaaa actccgcgcc ggggcaaatc ggcgtatccg
     2101 ccctccgtgc gacactcgac tgcctcgaaa acggcgcaga ctgatttcag acagcatcaa
     2161 aacatgatga aactcaatcc ccaacagctc gaagccgtcc gctacctcgg cggcccactg
     2221 ctcgtccttg ccggtgcagg cagcggcaaa accggcgtga ttactcaaaa aattaagcat
     2281 ttgattgtca atgtcggcta cctgccgcat accgttgccg caattacctt taccaacaaa
     2341 gccgctgcgg aaatgcagga gcgcgttgcc aaaatgctgc ccaaaccgca aacgcgcggg
     2401 ctgacgattt gcacgttcca ctctttgggc atgaagattc tgcgcgaaga ggcgaaccat
     2461 attggttaca aaaaaaactt ctccattctc gattctaccg acagcgcgaa aatcatcggc
     2521 gaactcttag gcggtacggg caaagaagcc gtattcaagg cgcagcacca gatttccttg
     2581 tggaaaaacg atttaaaaac gcctgaagat gtcgttcaga cggcatcgaa catttgggaa
     2641 caacaaaccg cacgcgtgta tgcgagctat caggaaacct tacaaagcta tcaggcagtg
     2701 gacttcgacg acttaatccg cctgcctgcc gtgctgttgc agcaaaacag cgaagtgcgc
     2761 aacaaatggc agcggcggct gcgttatctg ttggttgacg aatgccaaga tacgaatacc
     2821 tgccaattta cgttgatgaa gctgctgacc ggcgcggaag gtatgtttac cgccgtcggc
     2881 gacgacgacc agtccatcta cgcatggcgc ggtgcgaaca tggaaaacct gcgtaaaatg
     2941 caggaaaact atccgcagat gaaggtcatc aaactggagc aaaactaccg ctccaccgcg
     3001 cggattctca aaatcgccaa caaagtcatc gaaaacaacc ccaagctgtt taccaaaaaa
     3061 ctttggtcgc aattgggcga aggcgagccg gtcaaagtcg ttgcctgcca aaacgagcaa
     3121 cacgaagccg actgggtcgt cagccaaatc gtcaaacaaa aactcatcgg cggcgacaaa
     3181 acccaatatg ccgatttcgc cgtgttatac cggggaaagc atcaggcgag gattttcgag
     3241 gaagcattgc gcggcgcgcg catcccctac cagctctccg gcggacaaag ctttttcgac
     3301 aaagccgaaa tcaaagacgt gttgtcttat gtgcggctgc ttgccaaccc caacgacgat
     3361 cccgcctttc tgcgtgccgt taccacgccc aaacgcggca tcggcgatgt cacgctgggc
     3421 aagctcaaca cttacgcgca cgaacacgaa tgcagcctgt atgaagccgc gcaaaacgaa
     3481 gaagcccttg ccacgctgaa caataccaac cgccaacacc tgcaaacctt tatggatatg
     3541 ttcgtcagct acctcgccaa agccgaaacc agcgaagcgg gcgagttcat caacagcctg
     3601 ctcgaagaaa tcgactatga aaaccatttg atgcaaaacg aagaaggcaa agccggcgaa
     3661 atcaaatggc gcaacgtcgg cgatttggta tcatggtttg cgcgaaaagg cggggaagac
     3721 ggcaaaaaca tcatcgaact cgcccaaacc gtcgccttga tgacgctttt ggaaggaaaa
     3781 gacgaagaag aaaccgatgc cgtctcgcta tccacgctac acgccgccaa aggtttggag
     3841 tatccgtatg ttttccttgt cggttgcgaa gaaggcgttt tgccgcacaa cgacagtatc
     3901 gaagagggca acgtcgaaga agaacgccgc ctgatgtacg tcggcatcac ccgcgccaaa
     3961 cgccaactca cactgaccca ctgcgtcaaa cgcaaaaaac aaggcacatg gcagttcccc
     4021 gaacccagcc gattcataga cgaaatgccg caggaagatt tgaaaatcct ggggcgcaaa
     4081 ggcggcgaac cgattgtcag caaagaagaa ggcagacgca accttgccga tataatcgga
     4141 aggctcgaca acctaaaaaa aagcggcgcg gcggattaaa ccggagccgc aatgccgtct
     4201 gaaggcttca gacggcatat tttttggacg gcgcgcgtaa agcggtttac gcccacaaat
     4261 cctgctgctg gtttttcggc acaagatgcc ccacgccgat accgataagg cggaacgcgt
     4321 cttccgtctg cggcgagacg cgcgccatca acatttgcgc agcctgcagc agagctgcgc
     4381 agtcgggcaa tacggaggaa taagtcagtg tgcgcgtgat gatgcggaaa tcgtaggtct
     4441 tcagcttgag cgttacgctt tgggcttcga cgtttttgcg cgtgatttgc cgccacaagt
     4501 cttcggcaag atgggggagg tgtccggcag cctgctcgag cggcaggtct tcgggcaggg
     4561 taatttctgt ggagatttgg aggcgttcgc gttcggcttt gacggggcgt tcgtccgtac
     4621 cgcgcaccaa atcatagagg cggtatccgt agcgtccgaa atggtttaag agttcgccgc
     4681 gctcgaaacg gcgcaagtcg cccgccgtcc gcatacccag cgactgcatt tttttcagcg
     4741 ttaccttgcc cacgccgggg attttgccca aaggcagggt ttccaaaaat gccatgactt
     4801 tgtgcggcgg caacacaaac tgcccgttcg gcttgcgcca gtccgacgcg attttcgcca
     4861 gaaatttgtt cggcgcgatg cctgcggatg cagtcaaacc tgtttccgca aaaatggcgg
     4921 cacggatttc tttggcaacg tcgccggcgt aagggatgtt tttgaaatta cgggtaacgt
     4981 caagataggc ttcgtccagc gacaagggtt cgattaaatc ggtataacgc ctgaatacgg
     5041 cgtgaatctg cgcggaaacc tgacggtaca aatcgaaatg cggcggcaca tacaccgctt
     5101 gcggacacag ccttttcgcc gttgccaccg acatcgcgga atgcagcccg aactgccgtg
     5161 cctcatacga tgcggcgcaa atcaccgaac gcgcgccctc ccacgcgacg accaccggcc
     5221 gccctttcaa atgcggctgt tcgcgcagct ctaccgatgc gtagaatgcg tccatgtcga
     5281 tgtggataat tttgcgtgaa gacatcggct cttctgagga taaaagggat attctactgc
     5341 cggcatcggg caaattccaa atatacgccc cgatagacct gcctccataa aaatgccgtc
     5401 tgaaacatac cctgtttcag acggcatccg caaaactacg gttttcaatt aaaactgcca
     5461 atccagtttc atgctgacag tgcgcggctc tccgtagaag ttgtttgcgc cgcgcgtacg
     5521 gttgtagttg ttctcaaaat aagtgcgtcc gtttaagttc gtaccgatga ggctcaattt
     5581 ggcgtgtttg cccaattcgt aacggacgaa accgtctatc agcccgtagc cgccctgcct
     5641 gatgttatac agactgcttg tgccgctttg tgcggacacg ccgccgccga cggtcagccc
     5701 cgtattcggt atatggaagc tcgttccgaa acggaatatg tgcacgggtg tgaaattgct
     5761 gaagttgtac gggtctgcac tggaattttt ggcaaggcgt tcggcgttga cttcggcggc
     5821 gtttttgtag cggctcttgt tgtaggtgta acccgcaaag actttccaat cttcgttcaa
     5881 ctcacccgac aactcgaatt ccgcacccct gctgaccact ttgcctatcg gtttggcaac
     5941 ggtttggaac gacccctgct tgccgcctgc tccgggaaca tagccgaaat cgacgaccgt
     6001 gcggtttttc tgttcgaggt aaaacaatgc gaacgaagca ttcagccgtc cttgcaagaa
     6061 cgcgcctttc cagcctacct catagtttgt gccgaccaaa ggcggtaaaa cggttttggc
     6121 actgacatcg acattatcct gctgtttgaa gattttggta taacttccgt aaatactctg
     6181 ttgcggtgtc aagtcatagg taatgcctgc atagggcgtc aatttatgac cttgcatctt
     6241 ggccgtgtaa tggtcctgat ccgccctaat gctcgatgcc gtctgaaaat cgcttgccgg
     6301 ctgcccatag cggacaggca tatctttggt ttgcgaagtc tcatagcgcg tgtagtgcag
     6361 cccgcccaaa aggtgcagtc ggccggttac gttgaaacgc gtgctggcag tcagcgaatg
     6421 ggttttgttg gtgttgaggt atttggcgta gttatacagc gcaggaacat ggtcgtctgc
     6481 cactttgacg gttttccaaa ccggcaccgt accggaaaaa ccggtaaagg caggcgtgcc
     6541 gtcgggattg gtctcctgaa tcttgttgcc tttttcgtcc agctcatata catcgacata
     6601 taccggtgtc cggctgccgc tgtattcgtc atagtaatac acctgcttgc cttcggcatc
     6661 gagcttgggc tcggttttta ttttcttggc gttcctgcat tcttcggcat aaacggtacg
     6721 gttgcctttt tcatcgtacg cctgccaatc gggttcttta tgccccctga ccaaaggaga
     6781 cgacaaatcg ccgtccggct cctcctgaca acttcccgca tacacgccgt gcgttgcccc
     6841 cgtattcgga cgtactctgt agcggcgttc gtagatttct agatattccg aacgtatctt
     6901 ttcatcaccg taggcatagc cgacaaagaa atcatgctcc cgcccgaaca gcccatatgt
     6961 gccggtcagg tcaagtttaa ttccccattg gcggtcgtct ttggtatgcc gcaacggcat
     7021 ataactgtat cgtcggttgg cggtagcctt ccgattaaag gaagagtcat acaggctgtt
     7081 tggaaaacgt tgtgccgcat tattaaagat gccctccttc gcaagggctt tatcgacaaa
     7141 ttccgccttg tcggcatcaa cgcccggatc cccccaagaa ccttgacaga taaagtccag
     7201 cgcgaaaggg tcactcatac acttgtcaaa accggctttg cgttctgcgg cacggcggct
     7261 gcgatactgt tcgaaagcag tattatcgaa acggttttta acaaaatcgt ctttgcgctc
     7321 ccggtattcc ttggcggttt catcacgata tgctttcagt ttctccaatg ccttatcttt
     7381 cggctcgaac gggatgactt cgtttttttc agtcaaaaag cctaccgcat cctcacccga
     7441 caaacccgcc gcatattcgt ttttcagaaa aaactgcccc accttcgcat cggattcatt
     7501 cttggtataa gacacttcgg cattgagctg ccaaccgttg tcaaacacat gtttgaatcc
     7561 tgagaaaagg ttgtatttgt cggcacttaa ccgcgaccaa tcctccccca aataagtgtt
     7621 gcgcggcagt tgcaaaggcc ggttgcaggc aggcgttgaa ctgaacgggg cagttttctg
     7681 attttcacag ggcaaaataa tgcccgaaaa atcaggaacc tccctactct tctgatacat
     7741 gccgcccaaa gtaagcacac tgctgtcgcc cgcatcggct tcggcaatgc cgtaaaccat
     7801 atgtttcctg ccccaaactc ggtctttaaa cgatttttta tactcttccg cacccaccaa
     7861 ccttccgcgt aaggtattcg ccttattcag gctgcctgaa acatccaaca ctgcacgccg
     7921 gctgccgcga tggtcggcgg tcagctctcc ggtatgtttg aaagaagcgg taggtcactt
     7981 acggatcaaa ttgacggttc ctcccggctc tgaattggat tgggtcaacc ccgttgcacc
     8041 ccgtacaact tcaatatggt cataaaccgc caaatcggta ctcggagaca cgtcgatttt
     8101 cgccgtatat cccgaacggc ctgcaacatt gacggtcata ccgtcttcac caatctgatc
     8161 aatatagaaa ccgcgtgaca aaaaccgcgt ctgcaagcct gaatcgcgca caacgttgac
     8221 acccgtcgtg tttttcattg cctcttcaag cgtatgcacc gccttatcgt caaggcggct
     8281 gcgcgtgatg acgctgaccg actgcggcgt atccttgccc gcaatcctca tacctgtggc
     8341 ggtggacatc cgatctatcg tataagaacg ggtcttttcg gtcttgccca acaaagcatg
     8401 agagccgcgt acattgaccg tatccagact gacggtattg ccgtctgaaa caggcacaac
     8461 accgtctgca aaagaaccac cgtaagccga taacagcata acggtcagaa ttttaagtga
     8521 aaaatgattt tgattcatag agacctctgt aatatgcaag tgtgcaaatc gtccaaaggc
     8581 tctcacaact gttttgattt tttatattaa ttgaaaaaaa gtaattctca attaaattta
     8641 tagataggat tgtattccca ttttgacaaa aaacaaacta ctccttaccg tttatttcaa
     8701 aaaacgataa cattgtattg aaaaatatcc gaatttaaat acagaccgcc aatgcagaaa
     8761 aaaacaccca aattggctat aatcccgaca aacacactca aggacaacaa catggcagcc
     8821 tcgcccgaag caaaattcac cgaagaaaag attttgtggg tcaaacacca cacgccgaaa
     8881 ctcatcactt tcgccatcag ccgtcccgaa tcctaccgct ttaaagccgg acagttctcc
     8941 cgactcggtt tctacgaagg ggaaggtttc atttggcgtg cctattccat tgtttccgca
     9001 gaatatgccg acacgctcga atattttgcc gtactcatcc aagacggccc catgtcggcc
     9061 cgtttcgcca aaatgcaaca gggcaacacc atcctgctcg ataaaaatgc caccggcttc
     9121 ctcctgcccg aacgcttccc cgacggcaag gatttggtga tgctctgcac cggctcgggc
     9181 atcgccccct tcctttccat tctcgaacaa cccgaaatcc gtcaacgttt cgataccgtc
     9241 aacctgatac attccgtatc ttttcccgaa gaattgattt tcaacgaccg actcgccgca
     9301 ttgactgaac atcccctggt aggcgaatac ggacactctt tccgtttcgt ccctgttacc
     9361 acccgtgccg ccaacccctc gggcttaagc ggaaaacgca ttccggaact cttaaaaaac
     9421 aacagcatcg aacaggcgct gcataccaag ttcaccccgg aatccacacg gtttatgatt
     9481 tgcggcaacc cggaaatggt caaagacact ttccaaacgc tgctcgacat gggttacgcc
     9541 atgcaccgca accgcattcc cggtcaaatc atgatggaaa acggcttcta aaaaccaccc
     9601 tgcttgtccg atgccttcgg atggacgggc aaaccgacac ggcacgaaaa ccgcgtcggc
     9661 aaaaatgccg tctgaaaaaa ttcagacggc atcttcggat acattacctg caaacggcaa
     9721 cacaccggca caaaccgatt aggcaatcaa cacggtgacg gctgtttaca tacttgccgg
     9781 ctttcaccaa ccgatatcga tttaaccgat ttccttaata tttttcctgt ccgttttaaa
     9841 cttcgcctta aacgcatccg gtaaatcttt atcgaaatac caaagcccgt catccatttc
     9901 caatgcgccg cccattccgt gcagaacgac tttttccccc gtcaaaggat cggtttcggt
     9961 aatttccacc tcatgcattt ttccccaatc caaaacaggc aacttgtgtg caaacgccaa
    10021 aacctgttcg tcagaacggc cgcacgcctt atcgcatcgg cctgaaacat atacgggaca
    10081 aggctcatcc tctgcataac cgtccggaag gataccggct gcggcaggac acaatccgta
    10141 tgtttccgcc cacgacgaca aagcccgtct cgctgccttt ttattggcaa ataatccggt
    10201 aggcggatta tccgtcacac cgtttttcaa agccgctgtt ttcgcattca acatgccgtc
    10261 tgaacctttt tcaaacctga cggtcgtaaa tgttttaagc agatttttgg cagacacata
    10321 acaatccgaa tgataacgcc cgaccaattc cgctttaatc ttatatgcat gcaggctgcc
    10381 caatgcggga aaaaaacgga cttcctccgt attgcaccaa tcaaacgggg cttttccgga
    10441 gtccaataaa gccgaaatct cgctatatac ccgttcaaac gtaccgatat aatttacttt
    10501 ccctccgccg tcgaaacaag ccagcacccc cataccgtca ggcaaaccgt acaactgttc
    10561 cctcaaccgt tcgggcagcg cggcaggcag cggtttcgga ttcatcaaac ggaaacactg
    10621 cctgatccat gcctcaaccc cgtgttccga cagactgtat tccaaataat cacacaatgc
    10681 cgatacatcc gccatcgcac gatgcctgtc ttccacaaca atccccaacc tttcgatgat
    10741 actgtccagg ctgtgcttgt aaaattgcgg atacagacac cgggacagct gcacactgca
    10801 caaagcaggc gatgaaaatc cgatacccgc acgatgaaac tcatgcttta aaaacgtata
    10861 gtcgaaacgg ctgttatgtg caaccagcac acaacccttc aataccgaaa acaactcgcc
    10921 ggcaatctct gcaaaaacag gcgcatcggc aaccatgccg tctgaaatcc ccgtcagccc
    10981 cgccacaaac tgcggaatcg gtttttgagg attaaccaac cactcatgcc tcaccaccct
    11041 tccctgctca aacttgacca aagccacttc ggttaccctg tcttcataca gattgccgcc
    11101 cgtcgattcc aaatcaacca cggcaacagg cattccaaac cgtaaaaata ccttttccag
    11161 caagggccag cgagaagcaa caatcatttt attctcttta aattcaaaca acaaaccaat
    11221 attttacact tttaaggcat ttcatccaac aaaacaattg acagaatccg atgattaccc
    11281 taaaattcga atctttcttg cag
//
LOCUS       DAWIVB010000047        10969 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWIVB010000047 DAWIVB010000000
VERSION     DAWIVB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.047"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(60..1616)
                     /gene="dnaA"
                     /locus_tag="WIX60_000902"
     CDS             complement(60..1616)
                     /gene="dnaA"
                     /locus_tag="WIX60_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ5305189.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            1908..2042
                     /gene="rpmH"
                     /locus_tag="WIX60_000903"
     CDS             1908..2042
                     /gene="rpmH"
                     /locus_tag="WIX60_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ5305190.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            2045..2410
                     /locus_tag="WIX60_000904"
     CDS             2045..2410
                     /locus_tag="WIX60_000904"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ5305191.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            2475..2696
                     /gene="yidD"
                     /locus_tag="WIX60_000905"
     CDS             2475..2696
                     /gene="yidD"
                     /locus_tag="WIX60_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ5305192.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            2868..4505
                     /gene="yidC"
                     /locus_tag="WIX60_000906"
     CDS             2868..4505
                     /gene="yidC"
                     /locus_tag="WIX60_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ5305193.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGRQSVCAAGECNIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(4586..5296)
                     /locus_tag="WIX60_000907"
     CDS             complement(4586..5296)
                     /locus_tag="WIX60_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ5305194.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTARA
                     HLKHLGVTTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQAFRALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQEIISQTIVQWRKR
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(5358..5948)
                     /gene="maf"
                     /locus_tag="WIX60_000908"
     CDS             complement(5358..5948)
                     /gene="maf"
                     /locus_tag="WIX60_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ5305195.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAIVLLNTVTGRMRRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            6096..6599
                     /locus_tag="WIX60_000909"
     CDS             6096..6599
                     /locus_tag="WIX60_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ5305196.1"
                     /translation="MSDPNLIDLEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            6633..6812
                     /gene="rpmF"
                     /locus_tag="WIX60_000910"
     CDS             6633..6812
                     /gene="rpmF"
                     /locus_tag="WIX60_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ5305197.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            6987..7514
                     /locus_tag="WIX60_000911"
     CDS             6987..7514
                     /locus_tag="WIX60_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ5305198.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            7603..8658
                     /gene="plsX"
                     /locus_tag="WIX60_000912"
     CDS             7603..8658
                     /gene="plsX"
                     /locus_tag="WIX60_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ5305199.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            8794..9045
                     /locus_tag="WIX60_000913"
     CDS             8794..9045
                     /locus_tag="WIX60_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ5305200.1"
                     /translation="MKTLEKRMKALDKRIMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            9072..9290
                     /locus_tag="WIX60_000914"
                     /pseudo
     CDS             9072..9290
                     /locus_tag="WIX60_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9413..9616
                     /locus_tag="WIX60_000915"
     CDS             9413..9616
                     /locus_tag="WIX60_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305201.1"
                     /translation="MPSEAAWAFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSIMCTLSVLGNHLFFAYNSRI"
     gene            9643..10605
                     /locus_tag="WIX60_000916"
     CDS             9643..10605
                     /locus_tag="WIX60_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ5305202.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAAHRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDTPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            10652..>10969
                     /locus_tag="WIX60_000917"
     CDS             10652..>10969
                     /locus_tag="WIX60_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ5305203.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRCAIRGQRRIPEHRLLLPALLGGWVGAYFGSMTFKHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         2802 a         3300 c         2565 g         2302 t
ORIGIN      
        1 ctatatcttt acccttcgga cggcatgggc aatatcatgt cgtctgaaaa cgttttccat
       61 cagttttgaa tcagaatcag cagcttttca taatcctgag ccaattccgg atcttcttcg
      121 cgcagtttcg ccactgccct gatgccgtgc ataacggtcg tatggtcgcg cccaccaaac
      181 gaatcgccga tagacggcag gctcaaagta gtcagttctt tggtcaggct catcgccacc
      241 tggcgcggac gggcaatgtt tcgtgtccgt ttcttaccga gcacatcgct gattttgatg
      301 cggtaatatt tcgccaccgc atcgatgatg atgtcggcgg tgatgacttt gtgcttctcg
      361 gcaataatgt cctgcaaagc ggtacgcgcc aaatcgatgt cgatgacggg acggttcata
      421 aagcggctgc tcgctccgac acgattaaac gcgccttcaa gctcgcgcac gttggaacgg
      481 atcagattgg caatgaacag cgcggcttcg tcttcgatac tgatgcccgc cgcttccgcc
      541 tttttctgca aaatggcgat gcgcatttcc aattcgggcg gctcgagttc caaagtcagt
      601 ccccatgaaa aacgggattt gaggcggtcg tccatgcctt cgattttcgc aggcaacaca
      661 tcgcaagtga ggatgagctg ttttttctcg ttgtggaaat ggttgtacag atagaaaaac
      721 tcttccatcg tacggtcttt gcctttgatg aactggatgt cgtcgataat cagcaggtcg
      781 tattgcttgt attgctgctt gaacacgtcg taagtgttgt tgcgaaccgc cttcataaag
      841 ctgcggatat agtcatccga atgcatatag cgcactttgg catcgggacg gtttttcagc
      901 agctcgttgc cgaccgcctg cacaaggtgg gttttgccca aacccgtgct gccatagagg
      961 aagaacgggt tgtaactctg ccccgggctt tccgcaatcg cctgcgccgc agccgccgca
     1021 aggcggttgc ccttaccttc taccaacgta tcaaacgtgt aatccggaga caggttggtc
     1081 tgctcgtaac gcgcctcttc cgcatcgcgc tgcacgtccg tccgtgcttt ggcaactgcc
     1141 accgattccg gccgggaagc agacccggca gcctgacgcg gctcgtgcgg caggtttttc
     1201 atacgttccg ccaaaatatc cgccgccgtt ttcgacgcag cgggtttgac aggctcttca
     1261 gacggcagct cgtccaacag aacctcctgc acgggcattc cctccgacac cgcatgcaag
     1321 gacggctcgg caggttcgac agcaccttca accgccgcca tctcataacg cacgccttct
     1381 cccggtttga atacgaaggc ggaacggccg gcagccaact cttccctcac cgcttctatt
     1441 tttccggcaa actggctctt gagcatattg caggcaaact ggttcttgcc gtacaccacc
     1501 catacgccac cctcctcacc aacggtaagg ggcgcaatcc attgcgcaaa ctgcccttga
     1561 ggcaacatat cgtgaagacg gcggaggcac agcggccaaa actctgctaa tgtcatggat
     1621 aggctcgaat cggtaaaaat gaaatcgaaa acaaagaaaa tataatattt tcaaaaagaa
     1681 aacaaatctg ttgaacgcac atcggttcaa aacgcgactg cccgattata ccgactcacg
     1741 aatattttat ccacaacccg tgcaaaaatt tatccacaga aaggcggcgg aaatccgcag
     1801 gcaatcgggc aatcttcctg caaagtttct atattgattg acaaaagcgg caaattggag
     1861 tgtaattcac ggtttaatta tctacccatt ctattttagg aaacatcatg aaacgcactt
     1921 atcaaccttc cgttaccaaa cgcaaacgca cccacggctt cctggtgcgc tccaaaacgc
     1981 gcggcggccg cgcagtattg gccgcacgcc gtgccaaagg ccgcaaacgc ctggcggtat
     2041 aattttggac taccgcttcg gaaggcagta ccgcttgttg aaaacggatg atttttcatc
     2101 cgtttttgca ttcagaaacc gccgcagccg cgacctgctg caagtttcgc gctcaaacgg
     2161 caacgggctg ggccatcccc gcatcggtct ggtggtcggc aaaaaaaccg ccaaacgcgc
     2221 caacgaacga aattatatga agcgcgttat ccgcgactgg tttagattga acaaaaaccg
     2281 gctgccgccg caggatttcg tcgtgcgcgt ccaccgtaaa ttcgacaggg ctaccgcaaa
     2341 acaggcaagg gcggaactgg cacaactcat gttcggcaac cccgcaaccg gatgcaggaa
     2401 acaggcatga tcagaacggt actctgcagg caaggttcag acggcaacgg gtttcccata
     2461 caaggaacat cccgatgaac ttcctattgt ccaaactcct gctgggactg atacggttct
     2521 accaatattg catcagcccg ctgattccgc cgcgctgccg ttatacgccg acctgttcgc
     2581 aatacgcggt cgaagcggtc aaaaaatacg gcgcattcaa aggcggccgg ctcgccatca
     2641 agcgcattgc acgctgccac cctttcggcg gacacggaca cgaccccgtt ccctgacccg
     2701 acgcaatatt caaattgcac gctttccttt tatttcccat cggtttctat ataatgccgt
     2761 ctgaagcttc gggcaggcgg cacgaccgcc gggtatgaag cccgccctta ttccccgtct
     2821 atcggaacac gcaacctgcg gcatttccga ccattcagga aactcttatg gattttaaaa
     2881 gactcacggc gtttttcgcc atcgcgctgg tgattatgat cggctgggaa aagatgttcc
     2941 ccactccgaa gcccgtcccc gcgccccaac aggcagcaca acaacaggcc gtaaccgctt
     3001 ccgccgaagc cgcgctcgcg cccgcaacgc cgattaccgt aacgaccgac acggttcaag
     3061 ccgtcattga tgaaaaaagc ggcgacctgc gccggctgac cctgctcaaa tacaaagcaa
     3121 ccggcgacga aaataaaccg ttcatcctgt ttggcgacgg caaagaatac acctacgtcg
     3181 cccaatccga acttttggac gcgcagggca acaacattct aaaaggcatc ggctttagcg
     3241 caccgaaaaa acagtacagc ttggaaggcg acaaagttga agtccgcctg agcgcgcctg
     3301 aaacacgcgg tctgaaaatc gacaaagttt atactttcac caaaggcagc tatctggtca
     3361 acgtccgctt cgacatcgcc aacggcagcg gtcaaaccgc caacctgagc gcggactacc
     3421 gcatcgtccg cgaccacagc gaacccgagg gtcaaggtta ctttacccac tcttacgtcg
     3481 gccctgttgt ttatacccct gaaggcaact tccaaaaagt cagcttttcc gacttggacg
     3541 acgatgccaa atccggcaaa tccgaggccg aatacatccg caaaaccccg accggctggc
     3601 tcggcatgat tgaacaccac ttcatgtcca cctggattct ccaacctaaa ggcagacaaa
     3661 gcgtttgcgc cgcaggcgag tgcaacatcg acatcaaacg ccgcaacgac aagctgtaca
     3721 gcaccagcgt cagcgtgcct ttagccgcca tccaaaacgg cgcgaaagcc gaagcctcca
     3781 tcaacctcta cgccggcccg cagaccacat ccgtcatcgc aaacatcgcc gacaacctgc
     3841 aactggccaa agactacggc aaagtacact ggttcgcctc cccgctcttc tggctcctga
     3901 accaactgca caacatcatc ggcaactggg gctgggcgat tatcgtttta accatcatcg
     3961 tcaaagccgt actgtatcca ttgaccaacg cctcttaccg ctctatggcg aaaatgcgtg
     4021 ccgccgcacc caaactgcaa gccatcaaag agaaatacgg cgacgaccgt atggcgcaac
     4081 aacaggcgat gatgcagctt tacacagacg agaaaatcaa cccgctgggc ggctgcctgc
     4141 ctatgctgtt gcaaatcccc gtcttcatcg gattgtattg ggcattgttc gcctccgtag
     4201 aattgcgcca ggcaccttgg ctgggttgga ttaccgacct cagccgcgcc gacccctact
     4261 acatcctgcc catcattatg gcggcaacga tgttcgccca aacttatctg aacccgccgc
     4321 cgaccgaccc gatgcaggcg aaaatgatga aaatcatgcc gttggttttc tccgtcatgt
     4381 tcttcttctt ccctgccggt ctggtattgt actgggtagt caacaacctc ctgaccatcg
     4441 cccagcaatg gcacatcaac cgcagcatcg aaaaacaacg cgcccaaggc gaagtcgttt
     4501 cctaaatgcc gcagcatgaa aaatgccgtc tgaaacctgt tcagacggca tttttattgc
     4561 ccacccccta tcggggcgga aatcttcaac ccgcatacat cacaaaaatc gtcgggcgtt
     4621 ttttcagatt gggcatttct tttctttttc gccactgcac gattgtttga ctgatgattt
     4681 cctgtgtcgg caaggtcaaa tccgtagccg tgcataaacg cgtttcagga tgcaggtttt
     4741 ccaccgcatc ggcaagcagc gcatcattgc ggtaaggcgt ttcaataaaa atctgcgtct
     4801 cgccgcactg gcgcgaacgc tgttccaaag cccgaaaagc ctgaatccgc tcgttttttt
     4861 cagacggcag atagccttta aacgcaaaac tctgcccgtt cgcacccgaa gccatcaaag
     4921 ccagcagcag gctggaaggc ccgaccagcg gacgcacttc aaaaccgtgt ttatgcgcca
     4981 atgccaccaa attcgcaccc ggatcggcca cagccgggca acccgcctca ctgacaatgc
     5041 ccatactgcg cccttcttgc aaaggtttca gcaattccgg caaagtcttc aaatccgtat
     5101 gttcattcaa cgtttgcaga ttcagctcgc ggataggcgt agtcacgccc aaatgtttca
     5161 aatgcgcacg cgccgttttt tccgcctcca cgacaaaatc cgtcagcccg acaatcgcct
     5221 gttgttcatg cggcaacagg cacggcgtgt caggcgtacc caaaggcgta ggaatcaaat
     5281 acaaaacagg agacatcatt ccctcactca tcggttaaaa atgccgtctg agcctttcag
     5341 acggcataaa cgggcagtta cagaacctcc acgccctcat ttttcaagaa atcgaccaga
     5401 cggaaaaccg gcaaaccgat taaagcattc ggatcggtac tctcaatcct ttcaatcagc
     5461 aatgcaccca aatcctcact cttcagcgca cacgaacaat aaaccgcatc aggctcgcgc
     5521 tccaaatagc ggaggatatg caactcgtcc aactgcctca tcacgaccac cgtcttatcg
     5581 atatgccgcc gcatcctgcc cgtaaccgta ttcaacagca cgatcgcgct gtaaaactca
     5641 atctccctgc cgctcaagtg catcagcatc ttttgcgcat tggcaaggtt catcggcttg
     5701 ccccactgcc tgccgtcgca ccacgccacc tggtccgcac cgacaatcaa cgcctctggg
     5761 aaacgcccgg tcaacgaccg cgccttaccc tcggcaaggc gcaatgccgt ctgaggggcg
     5821 gattccccca acatcggcgt ttcgtcaaaa tcgggggacg ccgcctgaaa ggcaatgccg
     5881 agcctttcca tctgttcgcg gcggaaaacc gaactcgtac ccaaaatcaa aggcagttcc
     5941 aaacccatcc catcctcctt accgttgaaa acacgcccga aggggcagta aaatccagcc
     6001 atgcgccgaa acacggatac ccgccttcgg cgtaccgcaa catttttctt aaaaatattg
     6061 acgttagaac atctaaatta tatcatatcc cgtttatgtc agaccctaat ttgattgact
     6121 tggaaatttt tgccgccgaa gggcagaacc tgcaaggcag ttttctgctg gaagaattgg
     6181 atgaacgcgt cagttcgcac gattatcccg ccgacaggca gaccaaaata tcgtttacac
     6241 tgaccggcgg tcgcgaccgg ctgcaacgcc tgttcctcga cctgaacgtc aaagccgata
     6301 tgcccctgat ttgccagaga tgtatcaaac ccatgccgtt catgcttgat gaaagcagcc
     6361 gtatcgtcct gttttccaac gaagagtcct tggacgaatc catgcttgcc gacgaagaac
     6421 tcgaaggcat actgattgaa aaagaactcg acgtgcgcac attggtagaa gaccaaatcc
     6481 tgatgtccct gcccttttcg ccgcgacacg aagactgcgg cgacaatggg acactggaag
     6541 aagtcaatcg ggacaaaccc aacccctttg ctgttttggc aggtttgaaa agcaattgat
     6601 taggacacag tttatttatc taggagcttg aaatggccgt tcaacaaaac aaaaaatccc
     6661 cttccaaacg cggtatgcac cgttcgcacg acgcgctgac cgcgcctgca ctgtctgtcg
     6721 acagcacaac cggcgaagta caccgcccgc accacatctc ccccaacggt atgtaccgcg
     6781 gccgcaaagt ggtcaaagcc aaaggcgaat aatccctatt cgactgactg aaaaagccag
     6841 aacattgcca tgcaattact ggcttttttt gcattggacg caccatccgt ccaaactttc
     6901 gccatacgtc aacacacagg ggcaaagcgt tccgtataat accccgtgaa aatattccaa
     6961 aagccccaac caccaaggaa attccgatga aacagaaaat ctggtacacc tacgatgaca
     7021 tccaccgcgt catcaaagca ttggcagaaa aaatccggaa cgccgacatc aaatacgatg
     7081 ccatgattgc catcggcggc ggcggcttta ttccggcacg tatgctgcgc tgttttctgg
     7141 aaattccgat ttatgccgta accaccgcct attacgacag cgacaacgaa ggacaggtta
     7201 ccgaagaagt caaaaaagtc caatggctcg accccgttcc cgaagccctg cggggcaaaa
     7261 acgtactcgt cgtcgatgaa gtggacgaca gccgcgtaac catggagttc tgcctgaaag
     7321 aactgctcaa ggaagacttc ggtacgatcg gagtcgccgt actgcacgaa aaaatcaaag
     7381 ccaaagcagg caaaatcccc gaaggcattc cctatttcag cggcatcacc gtagaagact
     7441 ggtggatcaa ctatccgtgg gacgcactcg acatcgacga acacaaccgc cttgccgagg
     7501 ccggccgagg ctgacccttt cagacggcat attttccgaa ccgatgccgt ctgaagcccg
     7561 cacgacccct gccgcagacc gaaaacctac cggagaaacc ctatgattac attggccgta
     7621 gatgccatgg gcggcgacca aggacttgcc gttaccgtac ccggcgcaac cgcattcctc
     7681 caagcacacc ccgatgtccg cctgattatg accggcgacg aaacgcaact gcgccaagcc
     7741 ctgaccgcgg caggcgcacc gatggaacgc atcgacatct gccataccac ccaagtcgtc
     7801 ggcatggacg aagccccgca atccgccctg aaaaacaaaa aagactcctc catgcgcgtc
     7861 gccatcaacc aggttaaaga aggcaaagcc caagccgccg tatccgcagg caacacgggt
     7921 gcgctcatgg caaccgcacg tttcgtcctc aaaaccattc ccggcatcga acgccccgcc
     7981 atcgccaaat tccttccttc cgacaccgac cacgttaccc ttgcactcga ccttggcgcg
     8041 aacgtcgact gcacgcccga acagctcgcc caatttgccg ttatcggcag cgaactcgtc
     8101 cacgcactcc atcctcaaaa aggacagccg cgcgtcgggc tggtcaacgt cggcacggaa
     8161 gacatcaaag gtacggacac cgtcaaacaa acctacaaac tgctgcaaaa cagcaaactc
     8221 aactttatcg gcaacatcga aagcaacggc atcctctacg gcgaagcaga tgtcgtcgtc
     8281 gccgacggct ttgtcggcaa cgtcatgctc aaaaccatcg aaggcgcggt caaattcatg
     8341 agcggagcca tccgccgcga attccaaagc aacctgttca acaaacttgc cgccgttgcc
     8401 gccctacccg ccctcaaagg gctgaaaaac aaactcgacc cgcgcaaatt caacggggcc
     8461 atcctgctcg ggctgcgcgg catcgtgatt aaaagccacg gcggcacaga cgaaaccggt
     8521 ttccgctatg ccctcgaaga agcctaccac gaagccaagt ccgccggcct ttccaaaatc
     8581 gaacagggcg tagccgaaca actcgccgca ctcgaaactg ccaaagccgt ccaaaacgaa
     8641 aatgtcggcg gtctgtaaca cacacgatgc cgtctgaacg cccccgcccc tttcagacgg
     8701 catccgcccg caccaaacct gcgggcgcgg acggcgatgc gcctgtccgg cacttcccaa
     8761 atatcgcctt gtaaaataag gagtatttga aaaatgaaga cattagaaaa acggatgaaa
     8821 gctctagaca aacggattat gaagttcgga aaatcccttg aaggcaggct tgatgcccgt
     8881 ctgattgaat ccgcattgga ttatattcat tattcggaac gttttttggc ttttgaaatc
     8941 ctgtgtactt atatcgaaga tttcgatgtc cggctgacgg aacaagaatc ccgggaaatt
     9001 tcttttatca acaaggaatt tgagatagaa agcacgtccg attaaccaat aaagccaatg
     9061 ggttgataaa catgaaaaca tcgacggtcg tttttggcgg attttttatg gcagacaacg
     9121 gagagcgaat ccaaatcccc gttttggaaa atcctgacat tagggaaatc aatcactttt
     9181 tttccgtatc aaattttgag aaaaaaaccg gcgtccttgt tttcagaatc atccccgagc
     9241 cggaatttgg caataccgaa ttaactgtct attttaaaaa aggatattat agtggattaa
     9301 caaaaaccag tacggcgttg cctcgccttg ccgtactggt ttttgttaat ccactatatc
     9361 agacgaaaac aaacacccgc gccaatagcc tgacggcaac ccggcaatca aaatgccgtc
     9421 tgaagcagct tgggctttca gacggcattt ccttcgctta aaacagcgta tcggcaaccc
     9481 cgccctgcct gtccacggca atctgcatct gaaaaccatc tgtatcccaa accacacccc
     9541 catccctgtt tccatcatgt gcaccctgtc cgtattgggc aatcatctgt ttttcgctta
     9601 caatagccga atctgaacca actctctaaa aaggccgttc ccatgcagta tgcaaaaatt
     9661 tccggcacag gcagctatct tcccgccaac cgcgtcagca atgacgacct tgcccaaaag
     9721 gtagatacct ctgacgagtg gattaccgcg cgcacgggca tcaaattccg ccatattgca
     9781 gccgaaaacg aaaaaaccag cgatcttgcc gccgaagcgg cgcaccgcgc gctggatgca
     9841 gccggattag acagcggcga aatcgatttg attatcgtgg caacggcaac gccggatatg
     9901 cagtttccgt ctactgcgac catcgtgcaa caaaaattgg gcatcaccaa cggctgcccc
     9961 gcgtttgacg tacaggcggt gtgcgccggc tttatgtacg cgctgaccac ggcaaacgcc
    10021 tacattaaaa gcggtatggc gaaaaacgcg ctggtcatcg gcgcggaaac cttcagccgc
    10081 attgtagact ggaacgaccg cacaacctgc gtattgttcg gcgacggcgc gggcgcggtg
    10141 gttttaagcg cgtcggacac gccgggcatc atccacagca aactcaaggc cgacggcaat
    10201 tatctgaaac tcttaaacgt ccccgggcaa atcgcctgcg gcaaagtttc cggttcgccg
    10261 tacatttcga tggacggtcc cggcgtgttc aagtttgccg tcaaaatgct gtccaaaatc
    10321 gccgatgacg ttatcgaaga agcaggttac accgccgctc aaatcgactg gattgttccc
    10381 catcaggcaa accgccgcat tatcgaatcg accgcgaaac atttaggttt gagtatggac
    10441 aaagtcgtcc tgaccgtcca agaccacggc aacacatccg ccgcatcgat tccgctggct
    10501 ttggatacgg gcatccgcag cggacaaatc aaacgcggtc aaaacctgct gctcgaaggc
    10561 atcggcggcg gtttcgcgtg gggcgcggtg ctgttgcaat attgaacccg atgccgtctg
    10621 aaacaggctt tcagacggca tttcccatat catgaagcgg caggctttct tcaaactgat
    10681 ggcgtgtgcg gcatttctgt ctgccgtttc gctgcgcctc cccgtattgg gcgcgtgtta
    10741 cgcaatattg tccctctatg cgtttgcact ttacggcatc gacaaacggt gcgccatacg
    10801 ggggcaacgc cgcattcccg aacaccgcct gctgctgcct gcattgctcg gcggctgggt
    10861 gggcgcgtat ttcggcagca tgacattcaa acataagaca gcgaaaaagc gttttgttgt
    10921 gctgttccgt ctgactgttt caggtaatgt cttggcgacc ctcatcctg
//
LOCUS       DAWIVB010000048        10913 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWIVB010000048 DAWIVB010000000
VERSION     DAWIVB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.048"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            40..138
                     /locus_tag="WIX60_000918"
     CDS             40..138
                     /locus_tag="WIX60_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ5305204.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(209..1594)
                     /locus_tag="WIX60_000919"
     CDS             complement(209..1594)
                     /locus_tag="WIX60_000919"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ5305205.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPSGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(1691..4081)
                     /gene="gyrB"
                     /locus_tag="WIX60_000920"
     CDS             complement(1691..4081)
                     /gene="gyrB"
                     /locus_tag="WIX60_000920"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ5305206.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINNYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            4351..5223
                     /locus_tag="WIX60_000921"
     CDS             4351..5223
                     /locus_tag="WIX60_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ5305207.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            5311..7347
                     /locus_tag="WIX60_000922"
     CDS             5311..7347
                     /locus_tag="WIX60_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ5305208.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAV"
     gene            complement(7699..7920)
                     /locus_tag="WIX60_000923"
     CDS             complement(7699..7920)
                     /locus_tag="WIX60_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305209.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFPRHSRESGNLERKI"
     gene            complement(7996..9510)
                     /gene="katA"
                     /locus_tag="WIX60_000924"
     CDS             complement(7996..9510)
                     /gene="katA"
                     /locus_tag="WIX60_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ5305210.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            9858..10709
                     /locus_tag="WIX60_000925"
     CDS             9858..10709
                     /locus_tag="WIX60_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ5305211.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
BASE COUNT         2340 a         3006 c         3043 g         2524 t
ORIGIN      
        1 cttgccggat gctgcaaaca acgccggttt gcattcctga tggcggtggt tttcttagac
       61 gaacgcccga acacgcagga atggataggc ttggggctgg ttacggcggg cgtgttgacg
      121 ctggcactga aacggtaaag ccgcaagaaa taaatgaaat gccgtctaaa aaactgtttt
      181 cagacggcat tttcgtttct gtccatcctc agcactcgac cacgcgcacg gatacgggga
      241 cggctttttt ccggagcgtg gcggcgagtc cggcaagcga ggtttctttg taggtcgcct
      301 tcatatcccg gccggtttgc agcatggttc ggatgacttc gtcgagcgag acttttttgt
      361 ccgtgccgtc ttccaaaagc gcgagcgtgc cgagtttgag ggctttttcg gcggcgatgc
      421 cgttgcgctc gatgcagggg atttgcacca gtccgccgac ggggtcgcaa gtcagcccca
      481 aatggtgttc catcgccatt tcggcggcgt tttccacttg tttgggcgtg ccgccgatga
      541 cttcggcgta tgcgcccgcc gccatcgaac acgctacgcc gacttcgccc tgacagccga
      601 catccgcacc ggaaatggag gcgttggtct tgtagaggat gccgattgcg cctgcggtga
      661 gcaggaagtt ttcgacgcgt tcctgtgtgg cgtgcggatt gaacttgcgg aaatagtgca
      721 atacggcggg aatgatgcct gccgcgccgt tggtcggtgc ggtaacgacg cgtccgccgg
      781 cggcgttttc ttcgttgacc gccatggcgt acaccatcgg ccagagctgg gtgttgacga
      841 tttcggtttc gcgcaggact ttgagcttgg cggcaagctg cggggcgcgg cggcggacgt
      901 tcaatccgct gggcagttcg ccgtccgcac ccaagccgcg tttgatgcag ccttccataa
      961 cctcggcaac ggcagcggcg cggcggcgga tttcggcttc gccgcatccg gcaagcgcgg
     1021 cttcgtttgc caacacgact tcggagatgt cgagccggtt cagacggcat cgggcaagca
     1081 gttcggcgca actggtatag ggatagggaa cggctttttc cgtttccgcc tgccggtcaa
     1141 aatcttcttc ggtaacgaca aagccgccgc cgaccgaata ataaacctgt tcattcaata
     1201 ccgtgccgtc tgaagcatag gcggtaaaac gcaggctgtt ggggtgtttg ggcagcactt
     1261 gattgccgag tatgttcagg tcgcggtcgg ggatgaagcg gatttcttgc ccgttgagcc
     1321 ggaggatgtg ctgcgtgcgg atgcgttcga ggcgttcggg aatgccggca agcgggatgt
     1381 cgtgcggcag gctgccttcc aaaccgagca tcagcgcgtc aaatgtaccg tgtccgtatc
     1441 cggtcagtgc gagcgagccg taaatgtcga tgacgatgcg aacagcctgt gcatccaaac
     1501 ctgccgcaaa ggcggcggct gccttcatcg ggccgaccgt atgcgaactg gaaggcccga
     1561 taccgatttt gaaaatatcg aaaatgctga tcatattttg ctccgacggt ttttcagacg
     1621 gcacaggttc cgtttgacca accaaaaagg agacgcggca cgatgcccgt ctcctttttt
     1681 aaaacggcac ttatgcgtcg atattttggg caatcagcgc gttgttttcg ataaaggcac
     1741 ggcgcggctc gacctcgtcg cccatcagcg taacgaacac ttcgtcggcg gcaatggcat
     1801 cttcgatgcg cactttcaac aggcggcgca cggcgggatc catcgtggtt tcccacagct
     1861 gctcggggtt catctcgccc aagcctttgt atcgttggat ggacatacct ttttgggcaa
     1921 cgctcatcaa gatgtccaaa gcggtttcaa agctgtccgc gtcgtacccg ttttcgcctt
     1981 tgtaaagctt ggcaccctcg ccgaccatgc ctttgagcgc ggcggcggtt tgggtgaggg
     2041 tttggtaggc tttgctgttg aggaacttgg gttcgatgta gctgaccatg acgttgccgt
     2101 gcagcttgcg cgtgattttg atgaaccggt gtccttcatg accttcgatg cgttcgaggg
     2161 cgacttcttt ttcgtcaagc agaccggaaa gttcggcaac ggctttatcg gcgttttcag
     2221 acgacgtcaa atcaatgggc gacgcgtgta gcatggcgcg caggacgagt tcgtctacga
     2281 agcggctttc ctgttcgatg acggtttttg ccaacaggaa ttgtttggcg gtgtcggcaa
     2341 gttctgcgcc ttcgatggtg cggccgtctg aaatgatttt ggctttttcc aaggcaagac
     2401 cgagcagcca ttggtctttt tccaactcgt ccttgaggta acgttcctgt ttgccgtatt
     2461 tcgctttata caaaggcggc tgggcgatat agatgtagcc gcgctcgacc agctcgggca
     2521 tttggcggta gaagaaggtc aggagcaggg tgcggatgtg cgcgccgtcc acgtcggcat
     2581 cggtcatgat gatgatgcgg tggtaacgca gtttttcggc attgaattct tctttgccga
     2641 tgcccgcgcc caaagcggta atcagcgtgg cgacttcttg gctggccagc attttttcaa
     2701 aacgtgcttt ttcgacgttc aaaattttac ctttgagcgg caaaatcgct tggaatttgc
     2761 ggtcgcggcc ttgcatggcg gaaccgcctg cggagtcgcc ctcgacgagg tagagttcgg
     2821 acagggcagg gtctttttct tggcagtcgg cgagtttgcc gggcagtccc aagccgtcca
     2881 tcacgccttt gcggcgggtg atttcgcgtg ctttgcgggc ggcttcgcgc gcgcgggcgg
     2941 catcgacgat tttgccggtg atgattttgg cttcgttcgg attttcttcg aggaagtcgg
     3001 tcagggcttg gctgatgact tcgttgacaa cggggccgat ttcgccggaa accagtttgt
     3061 ctttggtttg ggacgagaat ttggggtcgg gcagtttgac ggacaacacg caggtcaaac
     3121 cctcgcgcat atcgtcgcct gcggtttcca ctttggcttt tttggcgact tcgttggctt
     3181 cgatatagtt gttgatggtg cgggtcatca cttggcgcag tgcggtcagg tgagtaccgc
     3241 catcacgttg cgggatgttg ttggtgaaac actgcacgct ttcttgatag ctgtcattcc
     3301 attgcatcgc gcattcgacg ctcatgccgt ctttttcgcc gaacgcgtag aagatttttt
     3361 cgtgcaacgg cgtttttttg cggttcatgt attgcacgaa acccgccacg ccgccggaaa
     3421 gggcgaagct ttcgtgtttg ccgtcgcgct cgtcggtcaa ttcgatgtcc acgccgttgt
     3481 tcaggaagga aagttcgcgg atgcgtttgg caaggatgtc gaagctgtat tcgacgttgc
     3541 cgaaggtttc cgtactggcg aggaagcgca cggtcgtgcc ttttttatcg gaatcgccga
     3601 caattttcag cggctcttcg gtttcgccgc gcacgaagcg gacgaagtgt tctttgccgt
     3661 cgcggtagat ggtcagcgtt acccagtcgg acagcgcgtt gacgacggac acgcccacgc
     3721 cgtgcaggcc gccggagatt ttgtagctgt tgttgtcgaa tttaccgccc gcgtgcaata
     3781 cggtcatgat gacttcggcg gcggagcgtc cttctttcgg gtggatgccg gtgggcatac
     3841 cgcgcccgtt gtcggcgacg ctgacggaat ggtcggcgtg tatcgttacc gtgattttgt
     3901 cgcaatgtcc ggcgagtgct tcgtcaatgg cgttgtccaa tacttcgaac accatgtggt
     3961 gcagaccgct gccgtcctgc gtgtcgccga tgtacatgcc ggggcgtttg cgtaccgctt
     4021 ccaagccttc gagcacctga atgctgtcgg cgccgtattc ttcgtgtttt tgttcagtca
     4081 tattttttgc cggattttga aaagattttg cgatgccgcc aaaacaagtc cgcaccttgt
     4141 agaaaaagcg ggcgggacga catatataat tgtgtattat agccgatttt gccgcctaat
     4201 tcagcgttat ccgcatcagt gtgccgccgg gaaaagatga aacggtacgt ttgcctccgg
     4261 catcaggtcg gggattgtcc cgtaaagtgg caaaagcgtt tttttgccac taaaatctac
     4321 accctatact tttcggacag gggcgcggaa atggaaatat ggaatatgtt ggacacttgg
     4381 ctcggtgccg tcccgatacg tgcggaggcg gtcgaatccg tggcggcggt tgcggctttg
     4441 ctgctggcgc gcgcccttct gttgaatatc cacttcaaac ggcatccgga tttcggcatc
     4501 gaaagcaagc ggcggttttt ggttgccagc cgcaatataa cgctgctttt ggtgctgttt
     4561 tcgctggcat ttatctggtc ggcgcaaatc caaacgctgg ctttgtcgat gtttgcggtg
     4621 gcggcggcgg tcgtcgtggc gacgaaggaa ctgattatgt gtctgtcggg cagtatttta
     4681 aggtctgcca cccagcaata ctcggtcggc gactatatcg aaatcaacgg cctgcgcggg
     4741 cgcgtggtcg acatcaacct gttgaacacg ctgatgatgc aggtcggtcc gaaccccttg
     4801 gtcggacagc ttgcgggaac caccgtttct ttccccaaca gcctgttgtt gagccacccc
     4861 gtgcgccgcg acaatatttt gggcgactat gtcatccata cggtcgaaat ccccgttccc
     4921 atccatttgg attcggatga agccgtatgc cgtctgaaag ccgtactcga gcccttgtgc
     4981 gcgccctaca tccccgccat ccaacggcat ttggaaaacg tgcaggcgga aaaactgttt
     5041 atcacgcccg ccgccagacc gcgcgttacc cgcgtgccgt acgatgacaa ggcataccgc
     5101 atcatcgtcc gcttcgcttc ccccgtttca aagcggctgg aaatccaaca ggcggttatg
     5161 gacgaatttt tgcgcgtaca ataccgcctg ttaaatcacc ccgccggctc cgaaacactt
     5221 taactttccc cgaccgaccc catttccggc ttcagacggc atattgccga tatgctgtct
     5281 gaaacacaac acgcaaagga aacccatctt atgactgaca acgcactgct ccatttgggc
     5341 gaagaacccc gttttgatca aatcaaaacc gaagacatca aacccgccct gcaaaccgcc
     5401 atcgccgaag cgcgcgaaca aatcgccgcc atcaaagccc aaacgcacac cggctgggca
     5461 aacactgtcg aacccctgac cggcatcacc gaacgcgtcg gcaggatttg gggcgtggtg
     5521 tcgcacctca actccgtcgc cgacacgccc gaactgcgcg ccgtctataa cgaactgatg
     5581 cccgaaatca ccgtcttctt caccgaaatc ggacaagaca tcgagctgta caaccgcttc
     5641 aaaaccatca aaaattcccc cgaattcgac accctctccc ccgcacaaaa aaccaaactc
     5701 aaccacgatc tgcgcgattt cgtcctcagc ggcgcggaac tgccgcccga acagcaggca
     5761 gaactggcaa aactgcaaac cgaaggcgcg caactttccg ccaaattctc ccaaaacgtc
     5821 ctagacgcga ccgacgcgtt cggcatttac tttgacgatg ccgcaccgct tgccggcatt
     5881 cccgaagacg cgctcgccat gtttgccgcc gccgcgcaaa gcgaaagcaa aacaggctac
     5941 aaaatcggct tgcagattcc acactacctc gccgtcatcc aatacgccga caaccgcgaa
     6001 ctgcgcgaac aaatctaccg cgcctacgtt acccgcgcca gcgaactttc agacgacggc
     6061 aaattcgaca acaccgccaa catcgaccgc acgctcgcaa acgccctgca aaccgccaaa
     6121 ctgctcggct tcaaaaacta cgccgaattg tcgctggcaa ccaaaatggc ggacacgccc
     6181 gaacaagttt taaacttcct gcacgacctc gcccgccgcg ccaaacccta cgccgaaaaa
     6241 gacctcgccg aagtcaaagc cttcgcccgc gaaagcctga acctcgccga tttgcaaccg
     6301 tgggacttgg gctacgccag cgaaaaactg cgcgaagcca aatacgcgtt cagcgaaacc
     6361 gaagtcaaaa aatacttccc cgtcggcaaa gtattaaacg gactgttcgc ccaaatcaaa
     6421 aaactctacg gcatcggatt taccgaaaaa accgtccccg tctggcacaa agacgtgcgc
     6481 tattttgaat tgcaacaaaa cggcgaaacc ataggcggcg tttatatgga tttgtacgca
     6541 cgcgaaggca aacgcggcgg cgcgtggatg aacgactaca aaggccgccg ccgtttttca
     6601 gacggcacgc tgcaactgcc caccgcctac ctcgtctgca acttcgcccc acccgtcggc
     6661 ggcagggaag cccgcctgag ccacgacgaa atcctcatcc tcttccacga aaccggacac
     6721 gggctgcacc acctgcttac ccaagtggac gaactgggcg tatccggcat caacggcgta
     6781 gaatgggacg cggtcgaact gcccagccag tttatggaaa atttcgtttg ggaatacaat
     6841 gtcttggcac aaatgtcagc ccacgaagaa accggcgttc ccctgccgaa agaactcttc
     6901 gacaaaatgc tcgccgccaa aaacttccaa cgcggcatgt tcctcgtccg gcaaatggag
     6961 ttcgccctct ttgatatgat gatttacagc gaagacgacg aaggccgtct gaaaaactgg
     7021 caacaggttt tagacagcgt gcgcaaaaaa gtcgccgtca tccagccgcc cgaatacaac
     7081 cgcttcgcct tgagcttcgg ccacatcttc gcaggcggct attccgcagg ctattacagc
     7141 tacgcgtggg cggaagtatt gagcgcggac gcatacgccg cctttgaaga aagcgacgat
     7201 gtcgccgcca caggcaaacg cttttggcag gaaatcctcg ccgtcggcgg atcgcgcagc
     7261 gcggcagaat ccttcaaagc cttccgcggc cgcgaaccga gcatagacgc actcttgcgc
     7321 cacagcggtt tcgacaacgc ggtctgacgg cagggttgaa gtaaaaaata tggcggattc
     7381 gatagaaaaa catccgcacc gtcattcccg cgcaggcggg aatccagacc ggtcggtgca
     7441 gaaacttatc gggaaaaacg gtttctttag attttacgtt ctagattccc actttcgtgg
     7501 gaatgacgcg gaaaagttgc tgtgattccg gataaatttt cgcaacgttt aatttccgtt
     7561 ttacccgata aatgcccgca atctcaaatc ccgtcattcc ccaaaaacaa aaaaatcaaa
     7621 aacagaaatc ccatcattcc cgcgcaggcg ggaatccagg tctgtcggtg cggaaactta
     7681 tcggataaaa cggtttcttt agattttacg ttctagattc ccgctttcgc gggaatgacg
     7741 cggaaaagtt ttcgtgccgg tttgaaccgg tggggatggt gcgggaatga cggcggaaaa
     7801 gtgggctgtg gggcgggaat gacgggatat tttgcgttta ataaaaaacg cccactgaaa
     7861 cggcgggggc gggatgggga aatgccgtct gaagcggtca aacgacgttt cagacggcat
     7921 ttttatgccc ggttatttcc ggtagcggac ggcgcgggac aggatttctt caatttccat
     7981 ccacataatg cccccttaca gcaaaccggg ctggccgagt gcggggtcgg tcgcgcgggc
     8041 ggcttgtgcg tcttcgacag tcagtccgag ggcttttgcc acgccttcgc cgtatgccgg
     8101 atcgcaacgg tagcagttgc ggatatggcg gtatttgatg aagtcgggcg cgtcgcccat
     8161 tgcggcggcg gtgttgccga acaatgcctg tttctgcgcg tcgttcatca ggttgaacag
     8221 ggcgcgcggt tggctgaaat agtcgtcatc gtcttggcgg tagtcccagt gcgccgcgtc
     8281 gccgttgatt ttcaaaggcg gttcggcgaa gtcgggttgt tgctgccatt ggccgaagct
     8341 attgggttcg tagtgcggca ggctgccgta gttgccgtcg gcgcgtcctt gtccgtcgcg
     8401 ctggttgctg tgaacagggc aacgcggacg gttgacaggg atttggcgga agttcacgcc
     8461 caagcggtag cgttgcgcgt cggcgtaatt gaacaaacgc gcttgcagca ttttatctgg
     8521 gctggcgccg acaccgggaa cgaggttgct cggtgcgaag gcggattgtt ccacatcggc
     8581 gaagaagttt tcgggattgc ggttcaactc gaattcgccc acttcaatca gcggatagtc
     8641 ttttttcggc caaactttgg tcaagtcaaa cggatgatac ggcacttttt ccgcatcggc
     8701 ttcaggcatg acttggatgt acatcgtcca tttcggaaac tcgccgcgct cgatggcttc
     8761 gtacaggtcg cgctgatggc tttcacggtc gtcggcgatg atttttgcag cttcttcgtt
     8821 ggtcaggttt ttaatgcctt gttggctgcg gaaatggaat tttacccaaa agcgttcgcc
     8881 cgcttcgttc cagaagctgt aagtatgcga accgaagccg tgcatatggc ggtagccggc
     8941 tgggatgccg cggtcgctca tcacgatggt aacttggtgc agggcttcgg gcagcagcgt
     9001 ccagaagtcc cagttgtttg tggcagagcg catattggtg cgcggatcgc gtttgacggc
     9061 tttattcagg tcggggaact tacgcgggtc gcgcaggaag aacacgggcg tgttgttgcc
     9121 gaccacatcc cagttgcctt cttcggtata aaatttcaat gcaaaaccgc ggatgtcgcg
     9181 ttctgcatcg gctgcgccgc gttcgcctgc cacggtggtg aaacgggcga acatttcagt
     9241 ttttttgccg acttcgctga agattttggc gcgggtgtat ttggtgatgt cgtgtgttac
     9301 ggtaaacgta ccgaacgcgc ccgaaccttt ggcgtgcata cggcgttcgg gaatgacttc
     9361 gcgcacgaag tcggcgagtt tttcattcag ccacaaatcc tgcgccagca gggggccgcg
     9421 cggaccggcg gtcaggctgt tttgattgtc ggcaacaggc gcgccgttgt tcatggtcag
     9481 atgggttacg gggcatttgg aggtagtcat cgctcttgtt ccttttctca ggttggtcaa
     9541 atgggggtaa acggcttaca gtacgatttg gcggaaagcg tattcgtaac cggtttcttg
     9601 attgcaataa atttcttgaa tcgacatttt atttcccttt tgtaaaaact atggatgcga
     9661 ctatacgcca agattttcgc tattaaaact atgaaatcga tttaatatta ttataagcaa
     9721 tcggttcttg attttcgttt gttttttgtt atcgaacgga atccgaaccc gctcattaaa
     9781 accatttata atgcaatgac gctttgcggc attttttgcg ccgacaggct gaaaataaca
     9841 attttcccca cattatcatg accttactcg gaataaagct caaacagacc cagcagctca
     9901 accagcggct gcaacaatct ttgcgcgtat tgcagatgtc gggtatcgaa cttgaacgcg
     9961 aggtcgaaaa ctggctgtcg gacaaccccc tgctcgaacg caaagacacg gatgaatttt
    10021 ccgatgccga gttcagccat tacactgcgc ctgcccgtca aatcggcgga gacgaaggcg
    10081 aagatatgct gtccaacatc gccggcgagc aggatttcaa gcaatacctg cacgcgcaag
    10141 tatgcgaaca cccgctttcc gaccaagaag ccgcctgtgt ccacatcctt atcgatttcc
    10201 ttgacgagca gggttatctg accgacagca tcgaagacat cctcgaccat acgcccttag
    10261 agtggatgtt ggatgaagca atgctgcaac acgcgctgac cgcattgaaa aaattcgacc
    10321 cggcaggcgt ggccgccgcc gatttgaacg aatcgctgat actgcagata gaaagattgg
    10381 gcgaatgtgc tgccaaaccc gccgccctgc atatcgtccg aaacgccctc gacagcattg
    10441 acggcaaccg cagccaaacc ctcgcacgaa taaaaaaaca cctgccccaa accgacagcg
    10501 gcacactcga agccgcactc gacctcattg cttcgctcaa tccctttccc gccgccggtt
    10561 ttgcctcgtc cacgcccacg ccgtattctg acgaggcgct cgccaacctg ctggctttcc
    10621 gcggcatgga ggtttctcgc cgcaccattg ccaaatacag agaatccttt gagattccgg
    10681 cagcacacaa acgcaaaacc gcagaataat tgccgaataa tcttataaag acaacaaacc
    10741 aaaagccggc atttctgcga aagcgggaat gccgaatccg tccgcgcgga aacctgcatc
    10801 ccgtcattcc cgcgaaagag ggaatctaga aacgcaaagc tgcaagaatt tatcggaaat
    10861 gaccgaaact caacgaacct ggattcccgc tttcgcggga atgacggggg ttt
//
LOCUS       DAWIVB010000049        10892 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWIVB010000049 DAWIVB010000000
VERSION     DAWIVB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.049"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(69..452)
                     /locus_tag="WIX60_000926"
     CDS             complement(69..452)
                     /locus_tag="WIX60_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ5305212.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGSILQFVCNWGFPAAEKTCSVLKKIGAVHSAMLIHRAADALDKEIRRLQS
                     EGKNLKEMWDITSRQQNRLTAEQSG"
     gene            complement(510..1886)
                     /gene="argH"
                     /locus_tag="WIX60_000927"
     CDS             complement(510..1886)
                     /gene="argH"
                     /locus_tag="WIX60_000927"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ5305213.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRCRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(1905..2774)
                     /gene="galU"
                     /locus_tag="WIX60_000928"
     CDS             complement(1905..2774)
                     /gene="galU"
                     /locus_tag="WIX60_000928"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ5305214.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(2802..3401)
                     /gene="rdgB"
                     /locus_tag="WIX60_000929"
     CDS             complement(2802..3401)
                     /gene="rdgB"
                     /locus_tag="WIX60_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ5305215.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDTEVKNRESHRAQALAELLRKLAL"
     gene            complement(3394..3627)
                     /locus_tag="WIX60_000930"
     CDS             complement(3394..3627)
                     /locus_tag="WIX60_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305216.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSATKENRYV"
     gene            complement(3735..4268)
                     /locus_tag="WIX60_000931"
     CDS             complement(3735..4268)
                     /locus_tag="WIX60_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ5305217.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(4370..4828)
                     /gene="nudB"
                     /locus_tag="WIX60_000932"
     CDS             complement(4370..4828)
                     /gene="nudB"
                     /locus_tag="WIX60_000932"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ5305218.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            5039..5115
                     /locus_tag="WIX60_000933"
     tRNA            5039..5115
                     /locus_tag="WIX60_000933"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5073..5075,aa:Pro,seq:ggg)
     gene            5273..7099
                     /gene="mafB"
                     /locus_tag="WIX60_000934"
     CDS             5273..7099
                     /gene="mafB"
                     /locus_tag="WIX60_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ5305219.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VSDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            7102..7566
                     /locus_tag="WIX60_000935"
     CDS             7102..7566
                     /locus_tag="WIX60_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305220.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            7755..7937
                     /locus_tag="WIX60_000936"
                     /pseudo
     CDS             7755..7937
                     /locus_tag="WIX60_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
     gene            7949..8236
                     /locus_tag="WIX60_000937"
     CDS             7949..8236
                     /locus_tag="WIX60_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ5305221.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            8732..9988
                     /locus_tag="WIX60_000938"
     CDS             8732..9988
                     /locus_tag="WIX60_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ5305222.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            10019..10438
                     /locus_tag="WIX60_000939"
     CDS             10019..10438
                     /locus_tag="WIX60_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305223.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            10629..10853
                     /locus_tag="WIX60_000940"
     CDS             10629..10853
                     /locus_tag="WIX60_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ5305224.1"
                     /translation="MRLATKDLAEAIRKGQVRKSSFNTEQLRAIEKGESKIPDYTWHH
                     HQDTGRMQLIREGLHHDTGHIGWEAMNKGR"
BASE COUNT         2844 a         2366 c         2925 g         2757 t
ORIGIN      
        1 gaacagggtt gctggaatag tattgccatc ccagcagata cagtttgtcg gggtcttgcc
       61 aatattgttc atccagactg ttcagcagtg aggcggtttt gttgtcgaga tgttatatcc
      121 cacatttctt tcaggttttt accttccgat tggaggcggc ggatttcttt gtccaatgcg
      181 tctgccgccc gatggatcag cattgcgctg tgtaccgcgc cgattttttt cagaacggaa
      241 caagtctttt cggcggcggg gaaaccccag ttgcagacaa attgcagtat gctgccgtta
      301 ccgatatcgg cttctgtccg ccaaatcaaa accagctcct gttcccgccc gtccatgctt
      361 tcgagtttgc cgtcatgctg ttcaaaaagt ttgtccaccg cctgccacat catctgttcg
      421 aagcggtcgg cttgggtatc cgtatcggtc atcgtgttct tgccttttaa aaatgccgtc
      481 tgaacatttc ttcagacggc atttgggggt taagccaaca tttcccgcca gcgtttcact
      541 tggaagcgga cttgttccgg cgcggtaccg cccaagtggt tgcgggcgtt taagctgcct
      601 tcgggtgtca gcacgccgta aacgtcgtcg gcaatcaaat cgctgaaacc ttgtaagact
      661 tcgagcggca gttcgctcaa atcgacgccc gcttggtcgg cgtggcgcac ggcttgggcg
      721 acgacttcgt gggcatcgcg gaaaggcatg ccttttttga ccagataatc cgccaagtcg
      781 gtggcggtag cgaagccctg catcacggcg gcgcgcatat tgtcgggttt gacggttacg
      841 ccgcgcatca tatcggcgta aatccgcaac gtgtcgataa gcgtgtcggc ggtgtcgaac
      901 aagggttctt tgtcttcctg attgtctttg ttgtacgcca agggttggga tttcatcagg
      961 gtaatcagac cgataaggtg tccgatgacg cggccggatt tgccgcgcac gagttcgggc
     1021 acgtcggggt ttttcttctg cggcatgatg gacgaacctg tgcagaaacg gtcggcgatg
     1081 tcgataaagc cgaaacgcgg gctcatccac aaaatcaatt cttcagacag gcggctcagg
     1141 tgaaccataa ccagcgaggc ggcggctgtg aactcaatgg cgaaatcgcg gtcggatacg
     1201 gcatcgagcg agttctggca gatttgttca aagcccaata gctcggcggt gatttcgcgc
     1261 tgaatcgggt aggtcgtccc ggcaagggcg gctgcgccga gcggcatacg gttgacgcgg
     1321 cagcggcagt ccgccatccg ttcgttatcg cgtccgagca tttcgacgta ggcgagcata
     1381 tggtgtccga agctgacggg ctgggcgact tgcaggtggg taaagcctgg catgacggtt
     1441 tcggcgtttt gttccgccaa atccagcaat gccgtctgaa ggctttgaat caggctttgt
     1501 ataacggtaa tctggtcgcg cagccacagg cggatgtcgg tggcgacttg gtcgttgcgg
     1561 ctgcggccgg tgtgcaggcg tttgcccgcg tcgccgattt tgtcggtcag gcggcgttcg
     1621 atgttcatat ggacatcttc caaatcggac gaccattcga ttttgccgct gcggatttct
     1681 tcgaggattt ccgccatacc ccggcggatg tccgccaaat cgccttcgtc caacacgccg
     1741 gtttctttca gcatttgcgc gtgtgccagc gagccttgga tgtcccattc ggcaagccgt
     1801 cggtcgaaac cgatggaggc ggtgtattgt ttgacgagtt cggaaacggg ttcgttgaaa
     1861 cgtccggacc aggttttgtc gtgcataagg attccttgat ggggttattc ggtgcggtat
     1921 ttttccaaaa gccggcggaa gggttcgccg gtttcgggat gtttcagacc gtaggcgacg
     1981 gtggcttcga ggtagcccag tttgctgccg cagtcgtagc gcgtaccttc aaaggggtgc
     2041 gccaggacaa attcgtgatc gagcagcttg gcgatgccgt ctgtaagctg gatttcgttg
     2101 cccgcgccgc gcggaagatt ggttaagagg tcgaaaatgc gcggggtgag gatgtagcgt
     2161 ccaacaacgg caaggttgga gggcgcgtct tcgggcttgg gtttttcgac aatgccggta
     2221 atgcgttgga actgtttgag ctgttcggtt tcgacgatgc cgtatgagcc ggtttgcgat
     2281 gcttcaacgg tttctacgcc caaaatgctg ttgccgctgc gcccgtacac ttcgaccatt
     2341 tgtttgagcg cgcctttggg ggcatcaatc aggtcgtcgg caaggataac ggcaaagggt
     2401 tcgtctccga tggcggcgcg ggcgcacaag acggcgtgtc ccaagcccag tgcttccgcc
     2461 tgacggatgt agaggcaggt aatgttcggc ggcaggatgt tgcggacgtg ttccaacaat
     2521 ttgtctttat ggcgcatttc caactcggtt tcgagttcgt atgccttgtc gaaatggtct
     2581 tcgatgctgc gtttgttgcg tccggtaaca aacaccattt ccgtgcagcc ggcttccacg
     2641 gcttcttcta cggcgtattg gatcagcggc ttgtcgacga tgggcagcat ttctttcggg
     2701 ctggccttgg tggcgggcag gaagcgggtt cccatccctg cgacggggaa aacggctttc
     2761 cgtatgggtt tcattctttt tcctttgtat tgttttgatg tttaaagggc gagtttgcgt
     2821 aagagttcgg caagtgcctg cgcgcggtgg ctttcgcggt ttttgacctc cgtatccaat
     2881 tcggcggcgg ttttgccgtg ttcgggcaga taaaaatacg ggtcgtaacc gaaaccgttt
     2941 tgcccgagcg gcgtgtcgtt ccactgcccg tgccatacgc cctcggcgat aatcgggcgc
     3001 gggtcgtctt tatggcggac aaaaaccaat acgcagacat agcagcagct tttgtctgcc
     3061 ttgccgacaa gttcggcggc aagtttcagg ttgttggcgg tatcggattt gggattgtcg
     3121 cccgcgtaac gtgcggaatg gatgcccggc gcgccgttta aggcggcggc acagatgccg
     3181 ctgtcgtcgg cgagtgcggg cagcccgctg tatttggcgg catggcgtgc ttttgccagc
     3241 gcgttttcga caaaggtggg atagggttcg gggcattcgg gtatgccgaa tgcggattgc
     3301 ggcaatacgg tgatgctgta aggtttgaat aagttgccga actcttcgag cttgccggca
     3361 ttgccgcttg ccaaaacgat tttttccggt ttttcagaca tagcggtttt cctttgtggc
     3421 ggattgggcg gcgcgtaggg atttgtgccg caggtagagg gcgaggctgc cgatttgtcc
     3481 gaacagtgcg ccgaatgcga aaagaaagga ggcgacggcg aaggctttgc tgccgacggt
     3541 cagcagataa gcacccaaga ggacgagcag cataaatgcc agtgtgaaga gggcaacaga
     3601 caacagatag atttttcggc ggttcatggc gttcggtcgg aaacggtatg ttcggattat
     3661 agccgattgg gacggtattc cctagagctt ggaaaaatgc cgtctgaaac ggcgttcaga
     3721 cggcatggcg ggcgttatgc ctgtttgttc caacgttcga tggattcttt gatgactttt
     3781 ttcgcttctt ccgcatcgcc ccacgattcg actttggtcg tacctgcttt tttcaggtct
     3841 ttgtaatggt tgaagtggaa ctcgatttgt ttgatgagct gttgcggcaa atcggacaaa
     3901 gttttgtagg cgttgccgtt gttgcggtcg tcggcaggaa cgcagacgat tttgtcgtcc
     3961 acttcgccgt cgtcaacgaa tttcatcacg ccgataacgc gcgcttccaa gaatacgccg
     4021 gttgccaaag gttgttcggt aacgagcagc acgtccaatt cgtcgccgtc ttcgtccaaa
     4081 gtttggggaa tgaagccgta gttggtcggt ttggcgaaga tggcgggttc gacgcggtcg
     4141 agttggaatg cggcgagttt gcggttccac tcgattttgt ggttgctgcc ggcggggatt
     4201 tcgttgacga cgttgatgat gccgccgtcc acatcgccgg gggtcaggat ttggttgaag
     4261 tctgccattt ggtttccttt gtttgggaaa gtttgaagtt tgaaagtata gcacaaacgt
     4321 ccggctgaaa atgcgcccga tgccgtctga aagggtgtac gggcgcgtgt taccgtttgc
     4381 ccaaaaacct gcccagttcc agaatcgcgc gcctgttgga cggggaaaac actttttccg
     4441 ccgcttcttc caagccgaac cagccgtagg agacgtgttc ctcgggttgc aggacgacgg
     4501 gcgtatcgcg cgggatttcg gcagagaaga ggtgttcgcg gttttcaaac acgccttttg
     4561 gatagcggtg ccgccagtgg tggtagattt cgtaaaccgt gctgtcgtgc cagtcttgaa
     4621 gctgcccgtc cgccagcagg atgccggttt cttcccaaac ttcgcgcctt gccgtttggg
     4681 cgacggtttc gcccggttcg aggctgccgg ttaccgactg ccaaaatcct ttcggatgcg
     4741 tgcgttcgat gagcaggatg ccgccgtccc cgctataaag gacgaccagt gcggaaacgg
     4801 ggtatttgag cggttttgcc atcggcatct ttcggcgggc tgcggtaatg aaggggcgga
     4861 ttatagcaaa cgccgcacgt tatggcgttt atccttttcc gtatcctttt ttctgcacgg
     4921 atgggacgcg ccggtgtttg ccggtaaatt ttccgttgtg tcaaaaagat aagggcggtt
     4981 gtgattttaa tgcttgccaa agcgtcgggc ggaaactata atccgaaact tatcgagtcg
     5041 gagtgtggcg cagtctggta gcgcacttgc atggggtgca aggggtcgaa ggttcgaatc
     5101 ctttcactcc gaccaaaaat tccgaaagcc gctttcaaaa gcggcttttt tgccgtccgt
     5161 atgattatga tgtagagtac gcggcgacag acattcaaat gccgtctgaa aaccgttcag
     5221 atggcatctc tttatcttag tttcattccg taccatctta aggaacatca aattgggcat
     5281 ttcccgcaaa atatccctta ttctgtccat actggcagtg tgcctgccga tgcatgcaca
     5341 cgcctcagat ttggcaaacg attcttttat ccggcaggtt ctcgaccgtc agcatttcga
     5401 acccgacggg aaataccacc tattcggcag caggggggaa cttgccgagc gcagcggcca
     5461 tatcggattg ggaaaaatac aaagccatca gttgggcaac ctgatgattc aacaggcggc
     5521 cattaaagga aatatcggct acattgtccg cttttccgat cacgggcacg aagtccattc
     5581 ccccttcgac aaccatgcct cacattccga ttctgatgaa gccggtagtc ccgttgacgg
     5641 atttagcctt taccgcatcc attgggacgg atacgaacac catcccgccg acggctatga
     5701 cgggccacag ggcggcggct atcccgctcc caaaggcgcg agggatatat acagctacga
     5761 cataaaaggc gttgcccaaa atatccgcct caacctgacc gacaaccgca gcaccggaca
     5821 acggcttgcc gaccgtttcc acaatgccgg tagtatgctg acgcaaggag taggcgacgg
     5881 attcaaacgc gccacccgat acagccccga gctggacaga tcgggcaatg ccgccgaagc
     5941 cttcaacggc actgcagata tcgttaaaaa catcatcggc gcggcaggag aaattgtcgg
     6001 cgcaggcgat gccgtgcagg gcataagcga aggctcaaac attgctgtca tgcacggctt
     6061 gggtctgctt tccaccgaaa acaagatggc gcgcatcaac gatttggcag atatggcgca
     6121 actcaaagac tatgccgcag cagccatccg cgattgggca gtccaaaacc ccaatgccgc
     6181 acaaggcata gaagccgtca gcaatatctt tatggcagcc atccccatca aagggattgg
     6241 agctgttcgg ggaaaatacg gcttgggcgg catcacggca catcctatca agcggtcgca
     6301 gatgggcgcg atcgcattgc cgaaagggaa atccgccgtc agcgacaatt ttgccgatgc
     6361 ggcatacgcc aaatacccgt ccccttacca ttcccgaaat atccgttcaa acttggagca
     6421 gcgttacggc aaagaaaaca tcacctcctc aaccgtgccg ccgtcaaacg gcaaaaatgt
     6481 caaactggca gaccaacgcc acccgaagac aggcgtaccg tttgacggta aagggtttcc
     6541 gaattttgag aagcacgtga aatatgatac gaagctcgat attcaagaat tatcgggggg
     6601 cggtatacct aaggctaagc ctgtgtctga tgcaaaaccg agatgggagg ttgataggaa
     6661 gcttaataaa ttgacaactc gtgagcaggt ggagaaaaat gttcaggaaa taaggaacgg
     6721 taataaaaac agtaacttta gccaacatgc tcaactagag agggaaatta ataaactaaa
     6781 atctgccgat gaaattaatt ttgcagatgg aatgggaaaa tttaccgata gcatgaatga
     6841 caaggctttt agtaggcttg tgaaatcagt taaagagaat ggcttcacaa atccagttgt
     6901 ggagtacgtt gaaataaatg gaaaagcata tatcgtaaga ggaaataata gggtttttgc
     6961 tgcagaatac cttggcagga tacatgaatt aaaatttaaa aaagttgact ttcctgttcc
     7021 taatactagt tggaaaaatc ctactgatgt cttgaatgaa tcaggtaatg ttaagagacc
     7081 tcgttatagg agtaaataaa aatggcaatt tggaattatc ggtactacct tatcccatca
     7141 gctgctatca gaaataaatt taatgcaaat aaagatatca tacttgatga gtatcgatct
     7201 aatggttttc agaattttaa tgagaataaa agttttgaaa attactttat cgataatgat
     7261 gttatattat tatcaataat aaatgaagca aaaaaacagc ttaaattgaa agaatcttgg
     7321 gataaagacg caatcatgtt ttgtgataat tttggtaata gtcttaccgt ttggccagat
     7381 gatatagagt gcgaacttga tttaagattt gattatacta aatttattca gaaaaccatt
     7441 gattgggcaa taaaatataa ttgtctactt gtaatagaaa aaacaggaaa tgtagtttcc
     7501 cctaatataa ataatctgat gtatgaaata aaagcatatt tggaaagcaa gccgtggccc
     7561 atatgaaacc taaactcaac aagtaggatg tgtgcggaac gcacgtatgc ggttctcaag
     7621 gtttgagcta agaggccgtc tgaaaacaga aaaactgttt cagacgacct ttcttttaac
     7681 cagttgccac agcaaccgga caaaagcagc ctacctccac atccatatag gcaatacagg
     7741 ggagatattt tgtaaattct acgaatattt tacctgctaa acagggtagg atatggtatg
     7801 aagcgaacat tggcttaata aacactatgt caagatcgaa tcaggctggt actagattgt
     7861 tgtattccaa ttatggattg ctatatataa caactgatca ttatatctct gcaacacggt
     7921 ttgtagcttg gaaataggag tataacttat gcaattagag attatcggta gtaaaattta
     7981 tacggaacaa gattttcata atcaaatttc aaaaatattt tctatacaag attattatgg
     8041 gaacaatctt gatgctttat gggatttatt aagcacaaat gtagaacgac cgattacttt
     8101 ggtatggaaa gatgctatgt tctcaaaaaa tcaattagaa aatatattta ttgaaatcgt
     8161 aaatgttcta gaaagagtta agaaacaaga tgaggattat ggattcgaag aaaaatttaa
     8221 ttatatttta gagtaagtaa accctaatta cattacgtac acaggcttaa aactccccag
     8281 agccaattaa gcaagccgta acccatataa aacttaaact caacaagtag catgtgtgcg
     8341 gaacgtacgc atgcggttct taaagtttga gctaagaggc cgtctaaaaa cagaaaaacc
     8401 gtttcagacg gtctttgttt aacgccaccg atccagcggg ttacaaagcg cagtcaatgc
     8461 cgctgcgcct tatgcctccg aagcaatagg cagaacattt ggacacggtg aaaacaaaaa
     8521 cgaaaccgcc caagccgtcg gacatttcct tttaggagca gctattgccc gcgtcaacgg
     8581 tggtaatttt gctgccggcg gctcggcagc agttgcagct gaaaaggcgg cggaacatct
     8641 tgcccaacag tataacgacg gtaaaaccgc aatcgatccg caaacaggcg agttcaatgc
     8701 caacctgctg ccggaacata tcaaagagga aatcaaatca aagagcgggg tgattgcatc
     8761 gctgacgggc gcggccgtgg gcggcacgcc ggtagatgcg caaaccggag gtgcggtcgg
     8821 acagaatgcg gtggaaaaca acctctatct gacatcggaa gccttaaaga aggacaagca
     8881 gacagctcgt aaaatttatt ccgtcataaa agagcaagtc aagcatgaat gcagttccac
     8941 aggaagaatt accgaatgtc gtcaaaatat aggacgcatt atcgaattta cccaagacaa
     9001 acgctttgac agtaggttta aggacttaaa aaaagaatcc ttatattacc taaataaaca
     9061 tcctgattta gtagcctctt atttgaaggc tgaatacgaa aagctggata gggaagacaa
     9121 aagtatcctg caccgctaca tctcacccgg ggctgaaatc gtttcgggca gtttgggggt
     9181 tgttctttca ggagtagccg gaggcggatc ttgtgccgag actttcggct taggctgtgc
     9241 cgccgctttg gttggtgtaa cgtcttccta cgatcatgtc attactggga cgaaaaactt
     9301 cggaaaaaaa gccagcgagc aacgaccgac gattgcggtt caggccttga agcagttggg
     9361 gctgtcggag caggctgcgg aatatgttca gttctctata gatttgttca gtgtgggtaa
     9421 atcggggggc ggtataccta aggctaagcc tgtgtttgat gcgaaaccga gatgggaggt
     9481 tgataggaag cttaataaat tgacaactcg tgagcaggtg gagaaaaatg ttcaggaaac
     9541 gagaagaagg agtcagagta gtcagtttaa agcccatgcg caacgagaat gggaaaataa
     9601 aacagggtta gattttaatc attttatagg tggtgatatc aataagaaag gcacagtaac
     9661 aggagggcat agtctaaccc gtggtgatgt acgggtgata caacaaacct cggcacctga
     9721 taaacatggg gtttatcaag cgacagtgga aattaaaaag cctgatggaa gttgggaggt
     9781 gaaaacgaaa aaaggtggga aagtgatgac caagcacacc atgttcccaa aagattggga
     9841 tgaggctaga attagggctg aagttacttc ggcttgggaa agtagaataa tgcttaagga
     9901 taataaatgg cagggtacaa gtaaatcggg tattaaaata gaaggattta ccgaacctaa
     9961 tagaacagca tatcccattt atgaatagta atatttatga aaaattagga gattaatgat
    10021 gaaaagaatt aagtgctttt gtgataaatt tccatcagga gatacattta gaatgtgtat
    10081 cattctggat gactatgata atagggttga ttattatgta ggaatatatg attacattac
    10141 gtctacctta atgagcgata tttactatcg atccacgatt gatgagcatt tcaagattat
    10201 agaattaata gaaaataatc caaatgaaat ttatgatgat ggcggtggtc aacaattttg
    10261 cctagaattt catcatgata aggtcatttt ttaccacaat gaatttgatg aagaagatgg
    10321 ttatccagta ttaagctgtt cgctgcatac ttttaaaact gctttaattg cttggaatgc
    10381 ttttttgcaa ttgcctaaaa gtattcattc ggtggtggag actgtgattg aggaataagc
    10441 ataattagct taatgaatag aatcagcgat atagattgga ctgcaaatcc acgcttatac
    10501 gctgtgccat gattaagatg ttagaacttg tattgaatac aagttctcat aaacgaatgg
    10561 cagtaagcat ttgatttaga taaaatcctt gaattagaat aatcaggtct aagagctcga
    10621 caggacaaat gaggctggca accaaggatt tggcggaagc cattaggaaa ggacaggttc
    10681 gcaaatcaag ctttaacaca gaacaattaa gggcaattga aaaaggagaa tctaaaatac
    10741 cggattacac ttggcatcat catcaagata caggaaggat gcaattgatt cgtgaaggct
    10801 tgcatcatga taccggccat attggttggg aagcaatgaa caaaggaagg taactatgtg
    10861 gaaaatcata aaagaggata gtgatgattt ag
//
LOCUS       DAWIVB010000050        10878 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWIVB010000050 DAWIVB010000000
VERSION     DAWIVB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.050"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(119..1336)
                     /locus_tag="WIX60_000941"
     CDS             complement(119..1336)
                     /locus_tag="WIX60_000941"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ5305225.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1536..2264)
                     /gene="rph"
                     /locus_tag="WIX60_000942"
     CDS             complement(1536..2264)
                     /gene="rph"
                     /locus_tag="WIX60_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ5305226.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMLREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2346..2810)
                     /locus_tag="WIX60_000943"
     CDS             complement(2346..2810)
                     /locus_tag="WIX60_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ5305227.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTAQELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3027..3803
                     /locus_tag="WIX60_000944"
     CDS             3027..3803
                     /locus_tag="WIX60_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ5305228.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            3941..4468
                     /locus_tag="WIX60_000945"
     CDS             3941..4468
                     /locus_tag="WIX60_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305229.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKICILRTSFI
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRSS
                     TDYIVSRNLKFRHLY"
     gene            4822..5676
                     /locus_tag="WIX60_000946"
     CDS             4822..5676
                     /locus_tag="WIX60_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ5305230.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPKQGAAYVVVNFIALLELAKEGLVRIVQEDGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5717..6925
                     /gene="pncB"
                     /locus_tag="WIX60_000947"
     CDS             5717..6925
                     /gene="pncB"
                     /locus_tag="WIX60_000947"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ5305231.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEAELDALCQLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPGIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            7017..8735
                     /gene="argS"
                     /locus_tag="WIX60_000948"
     CDS             7017..8735
                     /gene="argS"
                     /locus_tag="WIX60_000948"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ5305232.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPIVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALADNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9289..10707
                     /locus_tag="WIX60_000949"
     CDS             9289..10707
                     /locus_tag="WIX60_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ5305233.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2614 a         3066 c         2679 g         2519 t
ORIGIN      
        1 ttaatccact atattttttg ttccaaagtc aaaatatgcc gtccgaacat tcgggcggca
       61 gacaaaacgg cactgcccga taaaggcagt gccgttgtcc gtttcaaacc gtgaaacatc
      121 agcccaaatt aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcatcaa
      181 tcaatacgga aactttgatt tcggaggtgg aaatcatttg gatgttgata ccctcttcgg
      241 cgagcgtgcg gaagattttg gcggctacac cgacgtgcga acgcataccc aaaccgactg
      301 cggagacttt gcatacggtg tcgtcgccat caatagaagc cgcgccgata ctgtcttggc
      361 gttccgacag gatttccaaa gtctgcttgt aatcgccgcg cggtacggta aaggaaaaat
      421 cggttgtgcc ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatcgg
      481 caaccgcgcc taaaatctga taggcgacgc caggtttgtc gggtacgccg cgcacgttga
      541 tgcgggcttg gtttttatcg aatgcgatac cggttacggc agctctttcc atgttgtcgt
      601 cctcttcaaa ggtaattaag gtgccattgc cgccgtcttg caggctgctc agtacgcgca
      661 ggcgcacttt gtattttccg gcgaattcta ctgaacggat ttgcaaaact ttcgaaccga
      721 ggcttgccag ttcgatcatt tcttcaaatg taaccgtatc catgcggcgc gcttcgggta
      781 cgacgcgggg gtcggttgtg taaacgccgt ctacgtcggt atagatttgg cactcgtcgg
      841 ctttgagcgc ggcggcaagc gcgacggcgg aagtgtcgga accgccgcgt ccgagcgtgg
      901 aaatatcgcc ttcactgctg atgccttgga agccggcaac gatgacgact ttgccggcgg
      961 taaggtcggc acgcattttt tcgtcatcaa tgctttcgat gcgggctttg gtgtgggcgg
     1021 tatcggtttt gagggcgacc tgccagcctg tgtagctttt ggcatccacg ccgatgtctt
     1081 tcaatgccat cgccaaaagg ccgatggtta cttgttcgcc ggtagctaag acgacgtcca
     1141 gctcgcgcgg atcgggatgc tcttgcattt cgtgcgccag tgcgaccagt cggttggttt
     1201 cgccgctcat ggcggatacg acgactacga tgtcgtgtcc ttcggcgcgg gctttggcga
     1261 cacgtttggc tacgtttttg atgcgttcgg gcgagcctac tgatgtgccg ccgtatttat
     1321 gtacgattaa cgccatgttt cgtgctttct tgtgggggtt gtcgggcagc ttggtttgct
     1381 ggaaaaaggg ttattattac tattttttac atggaattca agaacggact gcgctttccc
     1441 gcctgccgtt tgacagcggt cagcgaaaaa cctgttcttt cagattgttg acaaaatgcc
     1501 gtctgaacgg ttttcagacg gcatccggac gacaatcagg cggcggacaa cgcattttgc
     1561 tggtgttgca gcagttcgcc tatgcctttt tgcgccagtg caaccagttt gcccaattcg
     1621 tccaaactga acggcgcgtc ttccgccgtc ccctgtattt cgatgatttt tcccgatgcg
     1681 gtcatgacga tattcacatc actgtcgcaa ccggagtctt cgggataatc caaatccaaa
     1741 agcggcacgc cgttcactac gcctactgac acagcggcaa cggcttcgcg gatggggttt
     1801 tcactcaaaa tgccgtctga aaccagtttg ccgacggcga tttgcagcgc gacaaacgca
     1861 ccggtaatcg aagccgtgcg cgtaccgccg tctgcctgaa tcacatcgca gtcaatcaag
     1921 atttgtcgtt caccgagttt ttccatatcc acgaccgcgc gcagggaacg cccgatcaaa
     1981 cgttggattt cttgtgtgcg cccggactgt ttgcccgccg aagcttcgcg gagcatccgg
     2041 gaagcagttg aggcaggcag catcccgtat tccgccgtta cccagccttg gtttttaccg
     2101 cgcagaaacg gcgggacgtt ttcatctatg gaagcggtac aaatcacttt ggtattgccg
     2161 cattcaataa ggcacgaacc gtccgtatgc ggcaggaaat gaggggtgat tttgatatcg
     2221 cgcaggctgt cggcggcgcg cgagatgcgg atgtaatcag gcatactgcc ctcccgttaa
     2281 aaacagataa attaaaaagc cttaaatatg aaaaatcaca tttaaggcct tcaaactgaa
     2341 aatttctacg cctcttcggc tttgctgcgg ataatcaaaa gcggcaggtg gctttggcgc
     2401 attaccgttt cggcaaaact gcccattaaa aggtgcatca gcccggtacg tccgtgcgta
     2461 cccaacacca gcaggtcggc accgttttca tcggcataat caaccaaatc ctgcgccatt
     2521 tcacgcgcac ccttattggc aaccagcagg tgtttgacgg tattttccac acccagttcc
     2581 tgggcggtgc gctcggcggc atccaaaact tcgttgcctt gcgcgacggc ggcggcttcg
     2641 tagctttcgt gttgcaaaaa ttcgggggcg agtgccatat attcggcagg attggcaacg
     2701 tgcaccaaag tcaggcgcgc accgttgacc ccggcaagct cggcggcatg tttcagggca
     2761 ttgatggacg tttcactgcc gtcaacggca acaaccaaat gtttgtacat atcgtattct
     2821 ccttttgcac cgcctcgcgg tgccctcttg tcggatgggc gcagggacag tttgcgctgt
     2881 ttcattatag acccgccgtc gggctttata caacagccga acagcccgac cgctttccag
     2941 tataatatgc cgcttccgtg cagtcaggca ttttttgccg gctttcgttc actttttgat
     3001 ttgacgcaat cttgcaggat tcgaccatgt ccgacaacgc tttgacctct tcgcgacgct
     3061 tcggcggcat cgccagactc tacggagact ctgccttggc gcacttttca caggcacacg
     3121 tctgcgtagt cggcgtgggc ggtgtcggct cgtgggcggt cgaggctttg gcgcggacgg
     3181 gcatcggacg tttgactttg attgatttgg acaacgttgc cgaatcgaat gtcaaccgcc
     3241 agctgcacgc cctgaccggc gacttcggca aagcaaaagt taccgccttg cgcgaacgca
     3301 ttacacaaat taatccgcaa tgcgaagtgt ttgaaattga agatttcgtt accgaagaca
     3361 atttgccgga atacttcgga aaaggttttg atttcgtcat cgacgcgatc gaccaagtgc
     3421 gcgtcaaagc agcaatggcg gcttattttg tggaacgcaa acaaccgttt gtcctcagcg
     3481 gcggcgcggg cggacaaaaa aatccggcgt taatccaaac cgccgatttg agccgcgtaa
     3541 cccacgaccc gctgcttgcc aacctgcgct acaccttgcg gaaacgctac ggattcagcc
     3601 gcgatacgaa agcaaatatg cgcgtgcctt gcgtgtattc gaccgaaaat atcgtgccgc
     3661 cgcagtctag ggaggcttgt tcggcagatg ccgctccgca aggcttgtcg tgcgccggct
     3721 acggtgcaag catgctcgtt accgcttcgt tcgggctata ttgcgcacag gcggcggtgg
     3781 aacacatcgc agacaaaaaa taagcaatgc cgtctgaaac aggattcaga cggcatttga
     3841 acaaactatg gttatgattt aagacaacaa aggatacgga taaaaaataa cataaaatat
     3901 atgattccta ataatatacc aagtatcgga gagctattta atggaattcg ttaataattt
     3961 agttattttt tcatttttat tactaatgct tattccgata ttttttgtag tatatggtat
     4021 ataccataag atacgttatc gcaaaatatg tatcctaaga acaagtttta tattattagt
     4081 ggtaatactt tgcagtatgt attacatata ttgccgttat cttgaccaac aaaaagtagc
     4141 ttattattgc atagatgaac aatgtatttc tattgttcat ctatacaaag attatggtat
     4201 aaactctccc acatatgcga gaatttacgc aggaaaaata ttgtttagat ttcaagtaag
     4261 agctaaaaat tacgctgaat tacttatgga agatgatata tcaattagta aaaaaatttt
     4321 ggggaataaa tttatcattt atgggtcgct acctgtaata tacggtaatg tagataatat
     4381 tgaagtaaaa gaagctactg gttatataga tagatccagt actgattata ttgtctcaag
     4441 aaacttaaaa ttcagacatt tatattaatt aagaggtttt agcaagagtg ccgtcaaaat
     4501 atagggcgca tcatcgaatt cgcgaaagac aaacgctacg atgaacgttt caaggatttg
     4561 aaaaaagaat ccataggcta tctgaaccgg catcccggtt tggtgtccga ctacctgaag
     4621 gcggcaatca agctgtcggt tcagaaaaac caacatcagc acgcctaaaa ccgtattcac
     4681 aacctgctcc ttttcaaaac atttgcattt aaaagccgtt ataatgccgt ctgaacatct
     4741 gcccgaccac attatacgtg aatgtcggca gattgttttc ttttgtaaac ttatattaaa
     4801 atccacttac cgattcacgc catgccgccc atccctgccc catctgcacc atccgagcac
     4861 actgtcgcat gggtattcgg ccaacccgtt accgatttgc cccaggattt gtttattccg
     4921 cccgatgcat tgaaagtcgt attgggcagc ttccaaggcc ctttggatct actgctgtat
     4981 ctgatccgca aacagaatat cgacgtactg gatattccga tggtgaagat taccgagcag
     5041 tatctgcact acatcgccca aatagaaacc tatcagtttg atttggcggc ggaatatctt
     5101 ttgatggcag caatgctgat tgaaatcaaa tcgcgcctgc tgctgccgcg taccgaaacc
     5161 gtcgaagacg aagaagccga cccgcgtgcc gagttggtgc gccgcctgct ggcttacgaa
     5221 cagatgaagc tggcggcgca gggtttggac gcgctgcccc gagccggacg ggatttcgcg
     5281 tgggcttacc tgccgctgga aattgccgtc gaagccaagc tgcccgaagt ctatattacc
     5341 gacttgacgc aagcgtggct gggtattttg tctcgggcaa aacacacgcg cagccacgaa
     5401 gtaatcaaag aaaccatctc cgtgcgcgcg caaatgacgg caatcctgcg ccgtttgaac
     5461 ggacacggaa tatgcaggtt tcacgacctg ttcaatccca aacagggcgc ggcttacgtg
     5521 gtcgtcaact tcatcgcact gttggagctt gccaaagaag gattggtcag aatcgtgcag
     5581 gaagacggtt tcggagaaat ccgaatcagc ctcaatcatg agggggcgca ttcagacggc
     5641 atttccggca cacgaggcgg gcgcgatgtg ttctaatacg ccccaagccg ccaccaaaaa
     5701 tcgggagaca cgccatatga ccggcatcat acattcgctg cttgacaccg acctctacaa
     5761 attcactatg ctgcaagtgg ttctgcacca gtttccgcag acgcacagcc tttacgaatt
     5821 ccgctgccgc aacgcctcga ccgtctatcc gcttgccgac atcagggaag acttggaagc
     5881 cgaactcgac gcgctctgcc aactacgctt cacccacgac gaactcggct atctgcgctc
     5941 cctgcgtttc attaaaagcg actttgtcga ttatctcgaa ctcttccagc tccaacgccg
     6001 ctttgtcgaa atcggcacag acgataaaga ccgtctgaac atccgcatcg aaggtccgat
     6061 gatacaggcg atgttttttg aaatcttcat cctcgccatt gtcaacgaac tttacttccg
     6121 ccgcctggaa acccctgcag tcatagaaga aggcgaacgc cggcttcaag ccaaagccgc
     6181 gcgcctcaaa gaaatcgccg ccgcacaaaa ccccgacgaa ccgcccttcc tgatttccga
     6241 cttcggcacg cgccgccgct acaagctcgc gtggcaggaa cacgtcatcc gcaccctgct
     6301 tgaagccgcc cccggcatcg tacgcggcac cagcaatgtc tttctcgcca aaaaactcgg
     6361 catcaccccc atcggcacca tggcgcacga gttcctgcag gcattccagg ccctcgacgt
     6421 acgcctgcgg aatttccaaa aggccgcgct cgaaagctgg gtgcacgaat accggggcga
     6481 tttgggcgtt gccctgaccg acgtggtcgg tatggatgcc ttcctgcgcg atttcgacct
     6541 ctatttcgcc aaacttttcg acgggctgcg ccacgacagc ggcgaccctt acgtttgggg
     6601 cgacaaagcc tacgcccact atcaaaagct caaaatcgac agccgcacca aaatgctgac
     6661 cttctccgac gggctggaca tcgaacgctc ttgggcattg caccaatatt tcaaagaccg
     6721 cttcaaaacc ggcttcggca tcggcaccaa cctcaccaac gatatggggc atacgccctt
     6781 gaatatcgtc ttgaaactgg tcgaatgcaa cgggcagtcc gtcgccaagc tgtccgactc
     6841 tccgggcaaa accatgacca acaacagcac cttcctcgcc tacctgcgcc aagtgttcga
     6901 cgtacccgaa cccgaaacgc cgtaaaccgg cagaaaaagc gcacaattcc tgtttctgcc
     6961 gcataaaatc ttttaaaata ccgcctgatt tgaatttaac cgaaagaccg aacttcatga
     7021 acctacatca aaccgtcgaa cacgaagccg ccgccgcctt tgccgccgca ggcatcgccg
     7081 acagccctat tgttttgcag ccgaccaaaa acgccgaaca cggcgatttc caaatcaacg
     7141 gcgtgatggg tgcggcgaaa aaagccaaac aaaacccgcg cgagttggcg caaaaggtcg
     7201 ccgaagcatt ggcggacaac gccgtgattg aaagcgcgga agtcgccggt ccgggcttca
     7261 tcaacctgcg cctgcgcccc gaatttctcg cgcaaaacat tcagacggcc ttgaacgacg
     7321 ctcgtttcgg cgtggcaaaa accgacaaac cgcaaaccgt cgttatcgac tattcttcgc
     7381 ccaatctggc gaaggaaatg cacgtcggcc acctgcgttc cagcatcatc ggcgacagca
     7441 tttcgcgcgt gttggcattt atgggcaata ccgttatccg tcaaaaccac gtcggcgact
     7501 ggggtacgca gttcggtatg ttggtcgctt atttggtcga gcagcaaaaa gacaatgccg
     7561 cgttcgagct ggcggatttg gagcagtttt accgcgccgc caaagtgcgc tttgacgaag
     7621 accctgcctt tgccgacacc gcacgcgaat acgttgtgaa gctgcaaggc ggcgatgaaa
     7681 ccgttttggc attgtggaaa cagtttgtcg atatttcgct ctcgcacgcc caagccgttt
     7741 acgacacgct gggcttgaag ctgcgtcctg aagacgtggc aggcgaatcg aaatacaacg
     7801 acgatttgca gcccgtggtc gatgatttgg ttcaaaaagg tctggcggtt gaggacgacg
     7861 gcgcgaaagt cgtgttcttg gacgaattta aaaacaaaga aggcgaaccc gccgcattta
     7921 tcgtgcaaaa acaaggcggc ggcttcctct acgcctccac cgatttggcg tgcctgcgct
     7981 accgcatagg ccgtctgaaa gccgaccgcc tgctgtacgt cgtcgaccac cgccaagccc
     8041 tgcacttcga acaacttttc accacttccc gcaaagcagg ctatctgccg gaaaacgtcg
     8101 gcgcggcatt tatcggcttc ggcaccatga tgggcaaaga cggcaagccg ttcaaaacgc
     8161 gcagcggcga caccgtgaaa ctggtcgatc tgctgaccga agccgtcgag cgcgccaccg
     8221 ctttggtgaa agaaaaaaat cccgaattgg gtgcggacga agccgctaaa atcggtaaaa
     8281 ccgtcggcat cggcgcagtc aaatacgccg acttgagcaa aaaccgcacc agcgactatg
     8341 tgttcgactg ggatgccatg ctctcgtttg aaggcaacac cgccccctat ctgcaatacg
     8401 cctacacccg cgtgcaaagc gtgttccgca aagcaggcga atgggatgca aatgcgccaa
     8461 ccgttttgac cgaaccgctg gaaaaacagc ttgccgccga gctgctgaaa tttgaagacg
     8521 tactgcaaag cgtggcggac acggcgtatc cgcactacct cgccgcctac ctctatcaaa
     8581 ttgcgaccct gttcagccgc ttctacgaag cctgtccgat actcaaagcc gaaggcgcaa
     8641 gccgcaacag ccgcctgcaa ctggcaaaac tcaccggcga cacgctgaaa caaggcttgg
     8701 atttgctggg catcgatgtg ttggacgtaa tgtaaaaccg caccgcccga ttgcggacaa
     8761 cagcctcgcc atccttatcc gaatctgaaa aaagcggcgc gatacaccgt atccgccgcc
     8821 cctcccaaaa tgcgaaacaa acaaacgcca agcaagcaag caagcaagca agcaagcaag
     8881 caagcaagca agcaagcaaa aaattataac ccccttcctg ccgacgcacg cactttccgc
     8941 gcggcgcatt ccccttttcc cgcccctcaa atccgccttt tcttcaggca gggtttcagc
     9001 ccgcctcttt tccctgtttt cctttccccg acacgcgtgc gctccccctg ccgcactgtg
     9061 ctgcactttc gcgcccggac ggcatcgttc cgccatccgg ttctctgttt tacatacccc
     9121 tgtttcagaa agaaatgcag atgtttcaac acacaggacg acacataaag caccgcccta
     9181 tgtgttgccc tgatttggaa ggggttacgc ctcccaaata aagtctgatc ctgccgcccc
     9241 gaaggacaga tgtccgagtg gcgaagtttc aaccgaaaag gaaatacgat gaatattcac
     9301 accctgctct ccaaacaatg gacgctgccg ccattcctgc cgaaacggct gctgctgtcc
     9361 ctgctgatac tgcttgcccc caatgcggtg ttttgggttt tggcactgct gaccgccacc
     9421 gcccgcccga ttgtcaattt ggactatctt cccgccgcgc tgctgatcgc cctgccttgg
     9481 cgtttcgtca aaattgccgg cgtattggcg ttttggctgg cggttttgtt tgacgggctg
     9541 atgatggtga tccaactctt cccttttatg gatctcatcg gcgccatcaa cctcgtcccc
     9601 ttcatcctga ccgcccccgc cccttatcag ataatgaccg ggctgttgct gctgtatatg
     9661 ctggcgatgc cgtttgtgtt gcagaaagcc gccgccaaaa ccgacttccg gcacattgcc
     9721 gtctgcgccg ccgttgtggc ggcagccggc tatttcaccg gccatttgag ttactacgac
     9781 cggggtcgga tggccaatat cttcggcgca aacaacttct actacgccaa aagtcaggcg
     9841 atgctctaca ccgtcagcca gaatgccgac tttattaccg ccggcctggt cgatcccgtc
     9901 ttcctcccct tgggcaatca acagcgtgcc gccacgcatc tgaacgagcc gaaatctcaa
     9961 aaaatcctct ttatcgtcgc cgaatcttgg gggctgccgg ccaatcccga acttcaaaac
    10021 gccacttttg ccaaactgct ggcgcaaaaa gaccgttttt cggtttggga aagcggcagt
    10081 tttcccttca tcggcgcgac ggtcgaaggc gaaatgcgcg aactgtgtgc ctacggcggt
    10141 ttgcgcgggt tcgcactgcg ccgcgcgccc gacgaaaaat ttgcccgctg cctccccaac
    10201 cgtttgaaac aagaaggtta cgccaccttt gcgatgcacg gcgcgggcag ttcgctttac
    10261 gaccgcttca gctggtatcc gagggcgggc tttcaagaaa tcaaaaccgc cgaaaacctg
    10321 atcggtaaaa aaacctgcgc cattttcggc ggcgtgtgcg acagcgagct gttcggcgaa
    10381 gtgtcggcat ttttcaaaaa acacgacaag ggactgtttt actggatgac gctgaccagc
    10441 cacgccgact atcccgaatc cgacattttc aaccacaggc tcaaatgcac cgaatatggc
    10501 ctgcccgccg aaaccgacct ctgccgcaat ttcagcctgc acacccaatt cttcgaccaa
    10561 ctggcggatt tgatccaacg ccccgaaatg aaaggcacgg aagtcatcat cgtcggcgac
    10621 catccgccgc ccgtcggcaa cctcaatgaa accttccgct acctcaaaca ggggcacgtc
    10681 gcctggctga acttcaaaat caaataacaa caatgccgtc tgaacgcacc aacagccttc
    10741 agacggcatt ttgcagacag accgaccctt caagcccact tttttcatca tctccgataa
    10801 attgctttgt atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag
    10861 agaacgattc tctaaggt
//
LOCUS       DAWIVB010000051        10737 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWIVB010000051 DAWIVB010000000
VERSION     DAWIVB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.051"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(13..216)
                     /locus_tag="WIX60_000950"
     CDS             complement(13..216)
                     /locus_tag="WIX60_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305234.1"
                     /translation="MDTTLKYKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKAKYDVQRQKLKEAGWSDDAV"
     gene            complement(194..565)
                     /locus_tag="WIX60_000951"
     CDS             complement(194..565)
                     /locus_tag="WIX60_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ5305235.1"
                     /translation="MAAYGVSDGIFTFTEQAVRIHRRFFGNFGYGGGRCYNNGLFLQG
                     NIMEATVYLEDNEYIALCDLLKLVGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRG
                     GEVIEFDGARLEIADGYDPEV"
     gene            965..4399
                     /gene="dnaE"
                     /locus_tag="WIX60_000952"
     CDS             965..4399
                     /gene="dnaE"
                     /locus_tag="WIX60_000952"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ5305236.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVRIGNPDAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLDGNEDAARTAALKY
                     AAWFPDAFYMELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQSNRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            4547..4696
                     /locus_tag="WIX60_000953"
     CDS             4547..4696
                     /locus_tag="WIX60_000953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305237.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(4833..5765)
                     /locus_tag="WIX60_000954"
     CDS             complement(4833..5765)
                     /locus_tag="WIX60_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ5305238.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEIPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMNNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFAAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSDCKRREERDMAGQAEQMAAELDRHG
                     AAVFFREYPNADHGNVPFYSLTDCVEYLRKAWQR"
     gene            complement(5835..7949)
                     /locus_tag="WIX60_000955"
     CDS             complement(5835..7949)
                     /locus_tag="WIX60_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ5305239.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTEGLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVRKRPTKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRRETFYAAADWDINPDTVLGAGYLYQQRRLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRKADSNYTFAGSKLNNTG
                     QADVAGLGTDIKQKAFAVDASYSRPFALGNTANEFVIGADYNRLRSTNEQGRSTLSKS
                     VALDGFRALPYNGILQNARAGNKGFNHSVTEENLDETGLYAKTVFRPLEGLSLIAGGR
                     VGHHKIESGDGKTLHKASKTKFTSYAGAVYDIDGSNSLYASASQLYTPQTSIGTDGKL
                     LKPREGNQFEIGYKGSYMDDRLNTRVSFYRMKDKNAAAPLDSNNKKTRYAALGKRVME
                     GVETEISGAMTPKWQIHAGYSYLHSQIKTASNSRDEGIFLLMPKHSANLWTTYQVTSG
                     LTIGGGVNAMSGITSSAGIHAGGYATFDAMAAYRFTPKLKLQINADNIFNRHYYARVG
                     SESTFNIPGSERSLTANLRYSF"
     gene            8109..8768
                     /locus_tag="WIX60_000956"
     CDS             8109..8768
                     /locus_tag="WIX60_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ5305240.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRTLLEMPSETAVADITRTYSAHYLNPNNRNMSLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(8856..9824)
                     /gene="ispH"
                     /locus_tag="WIX60_000957"
                     /gene_synonym="lytB"
     CDS             complement(8856..9824)
                     /gene="ispH"
                     /locus_tag="WIX60_000957"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ5305241.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNSHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(9853..10350)
                     /locus_tag="WIX60_000958"
     CDS             complement(9853..10350)
                     /locus_tag="WIX60_000958"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ5305242.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
     gene            complement(10556..10660)
                     /locus_tag="WIX60_000959"
                     /pseudo
     CDS             complement(10556..10660)
                     /locus_tag="WIX60_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2446 a         3051 c         2824 g         2416 t
ORIGIN      
        1 agccgaaccg tttcagacgg catcgtccga ccaacccgct tctttcaatt tctgccgttg
       61 cacgtcgtat ttggctttat ccgcccagta aatcgtctga atgcacgcct cgccgcagtc
      121 gctgccgcag cattcccacg actcgggtcg gacgggttcg tctaaaagcg gctcgcccaa
      181 aagggcttcg gctttatact tcagggtcgt atccatcggc gatttccaag cgagcgccgt
      241 caaactcgat gacttcgccg ccgcgtattt tggcggtttt acgggtttcg gtttcgccgt
      301 tgcgcaacac cagcccttcg gcgataaacg ctttcgcctg tccgccgctt tcggcaagtc
      361 cgaccaattt caagaggtcg cacaaggcga tgtattcgtt gtcttcgaga tagacagtgg
      421 cttccataat gttcccttgc agaaagaggc cgttattgta gcacctgccg ccgccgtacc
      481 caaaattacc gaaaaaccgg cgatgtatcc gcaccgcctg ttccgtaaaa gtaaaaatgc
      541 cgtctgaaac cccatatgcc gccatccgtt caaagaaatc ctgcccaacg gcagactgca
      601 aatcctgttc cccgacgaat ccgcattgac gctgatgcac atcctcaaac gcgaactgcc
      661 cgatacaccg gcaatcggca tcaaaacgaa atcaaaaacc tgtttgaacg taaaattaat
      721 ttaactgctt gccatccgtc cgaataaggc atatagttct ttataactag tttgatagtc
      781 ctttatatct atcaatactc cttgggaagc ctccgccata cggcaggagg catttttttg
      841 ccgtagtaaa agctcaaaaa catttgcagg tcatgccgtc tgaacccgaa acggcattac
      901 ctacaccgcc atctaaagac aaccctgcta caatacgcct tttattgtcc acgccgattt
      961 tgccatgacc gagccgacct acattcccct gcgcctgcat accgaatttt cgattaccga
     1021 cggtatggtg cggattaaaa aactgattgc caaagcgcag gaatacggtt tgcctgcttt
     1081 gggcatcagc gatttgatga acgaattcgg tttggtgaaa ttttataaag cctgccgcag
     1141 cgcggggatt aagcctatcg gcgcggcgga tgtgcggata ggcaatccgg atgcgcccga
     1201 caagccgttc cgcgctatgc tgattatccg taacgatgcg ggctatctgc gcttgagcga
     1261 gcttctgacg gcggcttatg tcggcaaaga ccgcaatgtc catcatgcgg aactcaatcc
     1321 cgaatggctg gaaaacggcg acaacagcgg cttgatttgt ttgagcggcg cacattacgg
     1381 cgaagtgggc gtgaatctgt tggacggcaa tgaagacgcg gcgcgtacgg cggcgttgaa
     1441 gtatgcggcg tggttccccg atgcgttcta tatggagctg caacgcctac ccgaacgccc
     1501 cgaatgggag gcttgcgttt cgggcagcgt gaagctggcg gaggaattgg gtttgccggt
     1561 ggtggcgacg catccgacac agtttatgag ccgcgacgat ttcaacgcgc acgaggcgcg
     1621 agtgtgtatc gcaggcggct gggtattgac ggacaagaaa cgtccgcgcg atttcacgcc
     1681 gggccagttt ttcattccgc cggaaaccat ggccgaacgt ttcgccgatt tgcctgaagc
     1741 cttggaaaac acggtagaaa ttgccaaacg ctgcaacctg cacatcacat tgggcaaaaa
     1801 cttcctgccc cttttcccca cgcccgacgg tttatcactc gatgactatc tcatcaaact
     1861 ctccaacgag ggtttgcagg aacgtatggt tcagctttat cccgacgagg cggagcgggc
     1921 ggcaaaaatg ccggaatatc aggaacgttt ggattttgag ctgaacatca tcatccaaat
     1981 gaaattcccc ggctatttcc ttatcgtaca agactttatc aactgggcga aaacacacgg
     2041 ctgtccggtc gggccgggcc gtggttcggg cgcgggttcg ctggtggcgt attcattgaa
     2101 gattaccgac cttgatccgc tcaaatacgc gctgctgttc gagcgtttcc taaaccccga
     2161 acgcgtttct atgcccgact tcgacgtgga cttttgccaa agcaaccgcg gccgcgtgat
     2221 tgaatatgtg cgcgagaaat acggcgcgga ggcggtcagc cagattgtta ccttcggcac
     2281 gatgtcgtcc aaagcggtca tccgcgacgt cgggcgcgtg ttagagctgc cgtttatgct
     2341 gtgcgacaaa ctgtccaagc tgattccgtt ggaagccaac aaacccctga gtttggaaaa
     2401 agccatggag accgagccac agattcagga attaatcgaa gcggaagaag cggacgaact
     2461 gattacgctg gcgaaaaagc tggaagattt aacgcgcggt ttgggtatgc acgcaggcgg
     2521 cgtgttgatt gcgccgggca agatttccga ttacagcccc gtgtatcagg cggacgaatc
     2581 cgcctcgccc gtatccatgt acgacaaggg cgacgtggaa gatgtgggtt tggtgaaatt
     2641 cgactttttg ggtctgcgca acctgaccat tatcgaaatg gcgcagaaca acatcaaaaa
     2701 cactaccggc gacatcatcg atgtcggcaa aatcccgctt gacgaccagg tcgcctacca
     2761 aatcttccgc gatgcgaaca ccaccgccgt cttccagttt gagtcgaccg gcatgaaaaa
     2821 aatgctgaaa acggcgcaca cgaccaagtt tgaagaactc atcgccttcg tatcgctcta
     2881 ccgccccggc ccgatggaca acattcccga cttcgtcgca cgtatgaagg ggcaagaatt
     2941 ccaatacatc catccgctac tcgaaggcat cctcgcgccg acctacggga ttatggtgta
     3001 tcaggaacaa gtgatgcagg cggcgcaaat tatcggcggc tactcgctcg gcggcgcgga
     3061 cctgctgcgt cgcgccatgg gtaagaaaaa acccgaagaa atggtgaaac accgcgaaat
     3121 cttcgccgaa ggcgcggcaa aacaaggcat ttcgcgcgaa aaatccgacg aaatcttcaa
     3181 ctacatggaa aaattcgccg gctacggttt caacaaatcc cacgccgccg cctacgccct
     3241 gatttcctac cagaccgcat ggcttaaagc gcactacccc gccgaattta tggcggcgac
     3301 catgtcgtcc gaattggaca acaccgacca gctcaagcat ttctacgacg actgccgcgc
     3361 caacggcatt gagttcctgc cgcccgacat caacgaatcc gactaccgct tcacgccgta
     3421 tccggacatg aaaatccgct acgcgctcgg cgcgattaaa ggcacgggcg aagccgccgt
     3481 cgaatccatc accgccgcgc ggcaaagcgg cggcaagttt accggtctgt tggacttctg
     3541 cgagcgcgtc ggcaaagaac acatgaaccg ccgcaccctc gaggccctga tacgcggcgg
     3601 cgcgttcgac agcatcgaac ccaaccgcgc catgctcttg gcgaacatcg acctcgctat
     3661 ggacaacgcc gaccaaaaag ccgccaacgc caatcagggc gggcttttcg acatgatgga
     3721 agacgccatc gaaccggtgc ggctcatcga cgcgccgatg tggagcgaat cggaaaaact
     3781 cgccgaagaa aaaaccgtca tcggctttta cctgtccggc cacccgttcg gcccgtatgc
     3841 ccaagaagtc cgccaaatcg caccgaccaa attagaccgt ctgaagccgc aagacagcgt
     3901 gcgcctcgcc ggattcgtta ccgccgtgcg tacgatgatg ggcaaacgcg gcaaaatcgc
     3961 cttcgtcagc ctcgaagatt tgagcggaca ggttgaaatc atggtcggcg gtcagacgtt
     4021 ggaaaactgc gccgactgcc tcaaagccga ccaagtgctg attatcgaat ccaaagtcag
     4081 ccgcgacgac tacggcggcg gcgacgggct gcgtattctg gcaaaccaag tcatgaccct
     4141 gcaaacggcg cgcgaacgct acgcccgcag cctcagcctc gccctcgccc cgcatcacga
     4201 catcggcgga ctggtacggc tgctcgccgc ccaccaactg cccgacacgc cgcgcatccc
     4261 gctgcaactg tcgtatgcca acgaaaaagc gtcgggcagg cttcaagtgc cgccgaaatg
     4321 gacggttaca ccgagctccg cattgttcgg cgaactggaa acattgctcg gcagccggtc
     4381 ggtgcgcgtc aactggtaac ccaaaatata aatgccgtct gaagcccaaa aaccggtttc
     4441 attcgtactt tattcgaatg attgaataaa agtaactgcc aagaaaaacg tattttttgg
     4501 ttatttcgcc agtctaaata gagcaaccgg gacgattgat atccgtgtgc atgacacaga
     4561 cagcaccaaa gggaaaaacg gcattttcca aagtatcggt atcaaaaccg ccctttcact
     4621 ccaaaaatac caaatcgaca aaccgggcaa agaaatcaga ccgtgccgcc tgaaaaaacg
     4681 cccacccgtc cgttaaatta acctatccct gtttcagacg gcctgaagca gggattttta
     4741 tatcaaaata aaatgagaaa gggagcaata acccttaggt agctcttgtt attttccgat
     4801 gcaaaacaaa gcagtcatat atttaattcc ccctacctct gccaagcctt cctcaaatat
     4861 tcgacgcaat cggtcagcga gtagaacggg acattgccgt ggtcggcatt cggatattcc
     4921 cggaaaaaga cggcggcccc gtgcctgtct aactctgccg ccatttgttc ggcctgccct
     4981 gccatatcgc gttcttccct gcgtttacaa tcgctacccc gttctagcgc gccgatgttg
     5041 aggcagacat cgatgccgtt tagccggttt tcagacggca taaagtcgag tatccgcctg
     5101 ttgtgccacc aaatcgatgg ggatacgagc caatgccgtc tgaaacggcg gtgggaaagc
     5161 agggaataca gtccgaacag tgcgccgaac gagtgtccga atacggcggt ttcattgcgg
     5221 ttgagggtgt agcggctttc taaaaaggcg gtcagctcgc tgtcgataaa ggcggcgaag
     5281 cggtctgcct gtccgaactg ctgccgttcg tctgctgtgg cgttgtctcc aagcggcggc
     5341 gtgtagtcgg cggcacgttg tgccaaatcg cgcacactgc ctgtcgtgta gccgataccg
     5401 acaatcaggc agggggcgtt gcttcgggta acggggttgt tcatcagcga ctgcatgatg
     5461 ttgaaaagtg cggggaaaaa ggcttcgccg tcgaggacaa agaggacggg atagccttca
     5521 gacggtattt cgccgagtgt tgccgtctga atccgataga ttcgccccgt gcaggtggat
     5581 ttgatttcgg tttcaaaggc ttggggcagt atggcaggtt ggaatgtgtc ggtcggtatg
     5641 ggtttcatga tgttcggctt gtgggtcaga ctggcggcaa gcggtaaaac cgaaccggca
     5701 aagcggtctg cctgccggtt cggtctattt tccttcgcaa tgccatactc cagttgtgag
     5761 agcatagggt atgccgcgca gcttgttgta gtttgatgat gctgcggctg ccgtttcaga
     5821 cggcatattg gtctttaaaa actgtaacgc aggtttgccg tcaggctgcg ctccgaaccg
     5881 ggaatgttaa aggtgctctc gctgccgacg cgggcgtagt aatggcggtt gaagatgttg
     5941 tcggcgttga tttgcagctt cagtttgggc gtgaagcggt atgccgccat cgcatcgaac
     6001 gtggcataac cgcctgcatg tatccctgca gatgaagtaa tgccgctcat cgcgttcacg
     6061 ccgccgccga tggtcagccc ggacgtaact tggtaagtcg tccacaggtt tgcgctgtgt
     6121 ttgggcatca gcaggaagat gccttcgtcg cgcgaattgg aggcggtttt gatttggctg
     6181 tgcaggtagc tgtaacctgc atggatttgc catttcggtg tcatcgcgcc gctgatttcg
     6241 gtctcaacac cttccatcac gcgtttgccc aatgcggcgt aacgggtttt tttgttgttt
     6301 gagtccagcg gtgcggcggc gtttttatcc ttcatgcggt agaacgaaac ccgggtattg
     6361 aggcggtcgt ccatgtagct gcctttgtag ccgatttcaa actggttgcc ttcgcgcggt
     6421 ttgagcagct tgccgtcggt gccgatgctg gtttgcggtg tgtagagttg ggaggcggaa
     6481 gcgtacaggc tgttgctgcc gtctatatcg taaaccgcgc cggcgtagct tgtaaatttg
     6541 gttttcgaag ctttatgcag ggttttgccg tcgcccgact cgattttgtg atgtcctaca
     6601 cgtccgcctg caatcaacga caaaccttcc agaggacgga acaccgtctt ggcatacaaa
     6661 ccggtttcgt cgaggttttc ttcggtaacg gagtgattga aacctttgtt tccggcgcgg
     6721 gcgttctgaa gtatgccgtt ataaggcaaa gcgcggaaac catctaaagc gacgcttttt
     6781 gacaaagtcg aacgcccttg ttcattagta ctgcgcaagc ggttgtagtc tgcaccaatc
     6841 acaaattcgt tggcggtgtt gcccaaggca aacggacggc tgtaacttgc gtcaaccgca
     6901 aaggcttttt gtttaatgtc cgtacccaaa cccgctacgt cggcttgtcc ggtattgttg
     6961 agtttgctgc ccgcaaacgt ataattggaa tcggctttcc gatcggaata gcgcataccg
     7021 actttgccgt agccgccgtt gccgaagtaa tgtttcaaat cggcgaacac gtcgtggctg
     7081 tgcattttaa atttgttcca atccgcgccg acaaatacgt gttgcggcag ggacggtaat
     7141 ttgttattgg catcggcagg caggccgttg tacggcgcga ggcggcgttg ctggtaaaga
     7201 tagcccgcgc ccaaaaccgt atcggggttg atgtcccaat ccgccgccgc gtagaaggtt
     7261 tcgcgccggt tgtttttctc ggcgggacgc ggagacgcgc cgacggtctg cgccatcacg
     7321 cggccgcgca cgctgccgtc tgaattgagg ctgcccgata cgtccgcctc ggctttatat
     7381 tgtttgtgcg taccgaaccc tgccgccgca tgaccttgga acgctttggt cgggcgtttg
     7441 cgcaccagat tcacgatgcc gcccatctcg ccgctgctgt cgaacagtcc gctcggcccg
     7501 cgcatcactt ccacgcggtc gaaggcgaac aggttgggca gcgtgccgtt gatactctgc
     7561 atctgcgcgg gcaggccgtc gatgttgtat tcgctgtatt cgtaaccgcg cgcgtaaacc
     7621 gaagagcgtc cgtcgtcgtt gctcaacacg cgcaggccgg gcgttttgcg tgccaactgg
     7681 tcaaacgtat caacattgcg gtctttgacc tgctggttgg taatgatgct gacggattgc
     7741 ggaatttcgc gcaaagaagc ggggattttt gtaccgacgg tggcggcaaa cgagctgtaa
     7801 tcgccgtttt tctcggtggc aatcgcgttg taagaacgct gacccttaat atggacggtt
     7861 tccaaacctt ccgtttgtgc ggcaaaaacc gaagacgaga gtgctgccaa aaccgtggcg
     7921 gcggtcatat tgatgcggaa aactgacata aactgtccca ttcacataaa tgataatggt
     7981 tctattttaa taaagcgcaa cgcggcttgt tcggaaaaac atatcgcgca accgacaaat
     8041 tttgtcgaaa atgcgacacg tctgcgtttt ccgcataaaa tttgcttttt tactgcaacc
     8101 aacctgctat gaccacgccc aaactcatca tcttcgactg ggacggcacg cttgccgata
     8161 cgacccaacc catcatcgac accatgcgcc gcagcttcgc cgaatgcggt tttccgccgc
     8221 ccgaagcgga acgcgtccgc agcctgattg gctacagcct gcccgaaatc atccgcaccc
     8281 tgctcgaaat gccgtctgaa accgccgttg ccgacatcac acgcacttat tccgcacatt
     8341 acctcaatcc caacaaccgc aatatgtcct tatttcccga tgccctgccc tgtctggaca
     8401 agctcaaagc acaaggatac tggcttgccg tcgccacggg caaagggcgg gcgggtttgg
     8461 acaacgccat cagtcaaacc gccaccggcg gctattggct cgccaccgcc tgcgcggggg
     8521 aatatccctc caaaccctcg cccgaaatgg tattcggaat ctgcggcgaa ctgggactcg
     8581 acccgaaaga ggcattggtc gtcggcgata cggcgcacga cctgcatatg gcggcaaacg
     8641 caggcgcggc ggcagtcggc gtggccaccg gcgcacattc gcgcgaacag ctccttagcg
     8701 caccgcatct cgccgtattg gacggtttgt ccgaactgcc cggttttctt gcacaacatt
     8761 acgcctgatt ggtttccgca tccggcacac ggcaaaaatg ccgtctgaag cctgttcaga
     8821 cggcatttgt gttgcccaaa cattcaacgc ctgcgtcaac gtttgcacaa atcgggtttg
     8881 gtttcgccct cgcggcgcaa ctctttgggc aggacgaaca caatgctttc ttccgcaccc
     8941 tcgccttcgc gtacggtttc gtgcccccat ccgcgtatgg ttgccagtac ttcccgcacc
     9001 aacacttcgg gcgcggacgc gcctgccgtt acgccgactt tgtttttgcc ctcaaaccat
     9061 gcgcgttgca ggtagcctgc attatccacc atatacgcat cgattccgcg cgatgccgcc
     9121 acttcgcgca agcggttgct gttggacgaa ttgggcgaac cgaccacaat cacgatgtcg
     9181 cactgttctg ccaactcttt gacggcggtt tgccggttgg tcgtcgcata gcagatatct
     9241 tccttgtgcg aattgcggat attggggaaa cgcgcgttca gcgcggcgat gatgtctttg
     9301 gtttcatcga ccgagagcgt ggtttggctg acataggcga gtttgtcggg gtttctgact
     9361 tcgagttttg ccacatctcc gaccgtttcg accaaaagca ttttgcccgg cgcaagctgc
     9421 cccatcgttc cttcgacctc gacgtgcccc ttatgcccga tcatgatgat ttcacagtct
     9481 tgggcatcca gtcgggcgac ttccttatgc actttcgtca ccagcgggca agtcgcatca
     9541 aacacgcgga aaccgcgctc cgccgcttct tgccgcaccg ccttcgatac gccgtgtgcc
     9601 gaataaacca gtgtcgcgcc cggcggcact tccgccaagt cttcaataaa caccgcacct
     9661 ttttcacgca ggttgtccac gacgaatttg ttgtgaacga cttcgtggcg cacataaatc
     9721 ggcgcgccga actcttccaa agcacgttcg acaatactga ttgcccgatc cacaccagcg
     9781 cagaagccgc gcggattggc aaggatgatg gttttctcgt tcataagccc ggtatttcgt
     9841 tttcagacgg catcaatatt tttcttcttg ggttttacgg tggacgatgt tgtccaacac
     9901 cgccaacacc gcaccgacgc agataaagct gtcggcaata ttaaaggcgg gataaaacca
     9961 attttgccaa taaaacaata agaaatcgac gacatgaccg tgtatcaggc ggtcgatgac
    10021 attgcctaac gcaccgccga taatcattgc cgcacccgtt ttgccgaggg ttgcaaactc
    10081 atcgcgcaag atggcgcgta ccaaatacgc gctcaccgcc accgccagca ccaaaaaaaa
    10141 gtatttttgc cagccgccct gatcggcaag gaagctgaac gccgcacccg ggttgtacac
    10201 cagcgtcaga tcgaaaaagg aaggaatgac attgacgcgt tcccgatact gaaacgacga
    10261 cagcaccgcc cacttcgacc actggtccag cacgatggcg gcaagtgcca atacccaata
    10321 gcgcgtttta cttgaaacag atgaagacat atttttcaac agccggtaaa agagtaccat
    10381 tttacccgaa aacccccttt cctgtacccg aaacggcaaa tgccgtaatc ttaaaacccg
    10441 tcattcccga caacaccgta atctcgaaac ccgtcattcc cgcgtaggcg ggaatccaga
    10501 cctgtccgca cagaaactta tcggataaaa acagttgccc aaaccccgcg ttctatagtg
    10561 gattaaattc aaaccagtac ggcattgcct cgccttgccg tactatttgt actgtctgcg
    10621 gcttcgttgc cttgtcctga tttaaattta atccactata gattcccact tccgtgggaa
    10681 tgacggttca gttgcattcc gacaacaccg taatcttgaa atccgtcatt cccgcgc
//
LOCUS       DAWIVB010000052        10672 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWIVB010000052 DAWIVB010000000
VERSION     DAWIVB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.052"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            328..1032
                     /locus_tag="WIX60_000960"
     CDS             328..1032
                     /locus_tag="WIX60_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ5305243.1"
                     /translation="MLRLVLAASLSAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADIQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(1096..1662)
                     /locus_tag="WIX60_000961"
     CDS             complement(1096..1662)
                     /locus_tag="WIX60_000961"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ5305244.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(1728..2144)
                     /locus_tag="WIX60_000962"
     CDS             complement(1728..2144)
                     /locus_tag="WIX60_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305245.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGNVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(2206..3105)
                     /locus_tag="WIX60_000963"
     CDS             complement(2206..3105)
                     /locus_tag="WIX60_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ5305246.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(3136..4593)
                     /gene="der"
                     /locus_tag="WIX60_000964"
     CDS             complement(3136..4593)
                     /gene="der"
                     /locus_tag="WIX60_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ5305247.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     EKFPEPEAEEADARHPVFAVIGRPNVGKSTLVNAILGEERVITFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(4743..5372)
                     /locus_tag="WIX60_000965"
     CDS             complement(4743..5372)
                     /locus_tag="WIX60_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ5305248.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RKVSQNQEAAAVLANIVEKAQSKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(5373..6668)
                     /gene="hisS"
                     /locus_tag="WIX60_000966"
     CDS             complement(5373..6668)
                     /gene="hisS"
                     /locus_tag="WIX60_000966"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ5305249.1"
                     /translation="MAQKIQSVKGMNDLLPVKQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEDSQNHYVRFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTPTLQQWKNA"
     gene            complement(6762..7418)
                     /locus_tag="WIX60_000967"
     CDS             complement(6762..7418)
                     /locus_tag="WIX60_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ5305250.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKE
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVLEKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGNLAGKILAVIPKKAETISNKLFFTQHPKRQTEFTVGQIRQARAE
                     "
     gene            complement(7700..8236)
                     /locus_tag="WIX60_000968"
     CDS             complement(7700..8236)
                     /locus_tag="WIX60_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305251.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(8404..8604)
                     /locus_tag="WIX60_000969"
     CDS             complement(8404..8604)
                     /locus_tag="WIX60_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305252.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTRA
                     EVYINGRRVYHNEAEMASASLR"
     gene            complement(8719..9138)
                     /locus_tag="WIX60_000970"
     CDS             complement(8719..9138)
                     /locus_tag="WIX60_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305253.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVDSP"
     gene            complement(9196..9561)
                     /locus_tag="WIX60_000971"
     CDS             complement(9196..9561)
                     /locus_tag="WIX60_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305254.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(9543..10091)
                     /locus_tag="WIX60_000972"
     CDS             complement(9543..10091)
                     /locus_tag="WIX60_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305255.1"
                     /translation="MKKQITAAMMILTMIAAPAMANSMDNQIFENQVFHTRADAPMQL
                     AELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIP
                     GGVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPG
                     QGIGRHRPWESKSTDRSWKNRF"
     gene            complement(10254..10526)
                     /locus_tag="WIX60_000973"
     CDS             complement(10254..10526)
                     /locus_tag="WIX60_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305256.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINIIIS
                     YVLIKNILVSVSDEGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
BASE COUNT         2700 a         2510 c         2447 g         3015 t
ORIGIN      
        1 tgcaaaaccg taagggtcgg tcaggatgct tgctgtttct ttccgcaagt agcggaacat
       61 cggtgcaagc acggcggttt tgatgccttt cctctgcaat gccagtgcct gattttgaaa
      121 aaaatcccgt ccacatcctg ttcggattgc ggataccatg aggggatgac gaggacgtgc
      181 aagggttcgg gcatagtggg attccgtatc ggaaaggcgg ttattataag acagacgcag
      241 accgaatatt taaattgttg ccttacgcta atgcaatttg gcgcgccggt gtgttagatt
      301 ggcagtttta tcggtaagga ggcggatatg ttgcgtcttg ttttggcggc ttcgctgtcg
      361 gcggtatctt ttccggcagc ggctgaagca ttgaattaca atattgtcga attttccgaa
      421 tcggcgggtg tcgaggtggc tcaggataca atgtccgcac gtttccaagt gacggcggaa
      481 ggacgggaca aaaatgccgt caatgctgag tttgttaaaa aattcaacaa gttcatcaga
      541 aaatcgaaaa atggtagctt taaaaccgaa ttggtatcgc gcagtgcgat gccgcgctat
      601 caatatacca acggcagacg cattcaaaca ggctgggagg agcgtgcgga atttaaggtc
      661 gaaggtagag attttgatga gttaaaccgt tttattgccg atattcaagc agatgccgcg
      721 ttggaatata cggatttcca tgtgtcgcgc gaacgccgca acgaggtcat cgatcaggtc
      781 agcaaggatg ccgttttgcg tttcaaggcg cgtgccgaaa agttggcggg cgttttgggt
      841 gcgtccggtt ataaaatcgt caaattgaat ttgggacaca tcggcagcca tatcgcggga
      901 gggggagctg ctcaggcaaa aatgcttcgt gccatgccga tggcggcaag cgtcaatatg
      961 gagggtgcgg attccgccgc gcctggtgtg gaggaaatca gcatcagcgt caatgggacg
     1021 gttcagttct gatttgaggt gaacggcaaa tgccgtctga aacccgacga taagggttca
     1081 gacggcattt atatttcagg ctttgggcag ggtaacgccg gtttgcccca tatatttgcc
     1141 gttgcggtct ttgtatgagg tttcgcagat ttcgtcgctc tcgaagaaga ggacttgcgc
     1201 cacgccttcg cctgcgtaga ttttggcggg caggggggtg gtgttggaaa attcgagggt
     1261 aacgtagcct tcccattccg gttcgaacgg ggtaacgttg acgataatgc cgcagcgggc
     1321 gtaggtggat ttgcccaagc agacggtcag gacgttgcgc gggatgcgga aatattcgac
     1381 cgtgcgcgcc agtgcgaagg aattgggcgg gatgatgcag cagtcgtctt caacggtaac
     1441 gaagtttttc gggtcgaagt ttttgggatc gacgatggtg ctgttgatgt tggtgaagat
     1501 tttaaattcg tttgcgcagc ggatgtcgta gccgtagctg gacgtaccgt aggagatgat
     1561 gcgtttgccg tcggcttctt tgatttggtt cggctcgaaa gggtcgatca tgccgaattc
     1621 ttcgctcatt cggcgtatcc atttgtcgga cttgatgctc ataatgtttt ccttgtttct
     1681 tgcagtgttc ggacaaagca ttgggggatg ccgtctgaaa acggggctta tttgtttttg
     1741 ggcagtttca cttctttaat catgccgttt tcgcatttca taatgaagag ggtttcgccg
     1801 ttttgcgaga cggtaaatct gccgtgtgcc aagccttttt tgaacgtgcc cgagagtacc
     1861 atgttgcgga atttggtact gtcggaattg aacggttcga taaatatttc gcggttggcg
     1921 gcaacggtat aaacgccttg cccgtcgaat ttgccgtttt taaacgaacc ggtatagttg
     1981 cgcccgtctt ggcagcgcca tgtgcctttg ccggcgggtt taccgtcttt gccgacattg
     2041 ccgtcgtagg tgcagcctgg ttcttgatag gaagtcagga cggcggccga agtggggagg
     2101 gcgaacatca tggcgggcag taggaatgcg agatgtttaa gcataagggt tattccattg
     2161 gattttggtt gacggtattt tgtcgtgaaa aagccgtctg aaaaatcaat cttgccagcc
     2221 gcccaaatag gaaaccagtt cttccaacat ggtgctgacg gattccgcca tcagaatttg
     2281 cgaggcgaag gcaaggccgg cggcatcgtc gccgttgctt tcggcttctt cctgcaatac
     2341 gtcgaggtat tggatgcgct tgagtgtgaa gtcttgagtg aggataaagg caatttgttc
     2401 gcgccacacc aagcccagtt gggtaacggt tttaccgttt ttgacgtgtt gaaccacttc
     2461 gtcggcggtt aaatcttgtt tggatacttt gacgacggga acaatatcgc ccgtaccttt
     2521 gagttcgcaa tcgctgtcta attcaaaacc gccttcgcaa tgcccttgca acagccagcc
     2581 ggtcatcaag gaagagggcg attgcttggt attcggcagc gaggcttcca aaccgcccaa
     2641 agcttcgcgc agcttggtca ggatgttttc tgctttggcg gaagccgcgt tattgacgag
     2701 caggtagccg tggcgggtgt taaacaccgc ttctgtacgg ctgctgcggg taaacgctcg
     2761 gggcagcagg tcgtctgtaa tttgctcttt aagctcttgt ttttctttac ggccgacatt
     2821 gcgggcttca ttgttttgga tttccgctac cttctcttcc aaaatatcgc ggatgacgcc
     2881 ggcaggcagg actttttctt cttttttcag ggcgacgcgc aaggtaaagt cggcagggaa
     2941 aacgagttca ggggagaatg aaaccggtgc ggtaaagcct tcgctgaacc agtctaagcc
     3001 ttggcaatgg gtaaattcag cttcagcaag tttgtcggca agtacgtctg cctcaggcag
     3061 cttttctttg ttgagcggat aaaaactaat ctgcttgaac cacataatgt ttcctattgt
     3121 ttgaaatgtc gggaattatt tgctgaattg ttttttcaca ctgactttgg ttttcttctt
     3181 gaagcggttt ttctcttcct ttcggccttc gcgtttctca atacggttgc tcaggctgac
     3241 gcggcgcagc ggttttttct tcggtttgtc ttccgcattt tcatacgggt tttccgaaac
     3301 attgtattga attctgagcg gcgtgccttg cagattgaag gctttgcgga acgtttgggt
     3361 cagatagcgc gtatagctgt cggaaatcgc gtgcagcgaa ttgccgtgta ccacaattac
     3421 gggagggttc atgccgcctt ggtgggcata acgcattttc ggacgcacca agccggcacg
     3481 cggcggttgc tgacgctcga tcgcgctttg taatacgcgc gtgattttcg gcgtcggcat
     3541 cttaatcatc gccgcgttgt aggcagcctg aatgctgtca aacaaaccgt ctataccgcg
     3601 ctctttcaat gcggaaataa agtggaactt ggcaaaatcg aggaaataca gtttgcggtt
     3661 gatatcgcgt ttcacttgct cgcgacgttc ttcgctgatg ccgtcccatt tattgaccgc
     3721 caccaccaaa gcacggcctg cctccaaagc gaaacctgca atcgtcgcat cttggtcggc
     3781 gatgtcctgc tgcgcgtcca ataccaaaac agcgacgttt gccgcttcaa ccgcctgcat
     3841 cgctttgata acggagaatt tttccactgc ttcatccact ttgccgcgac ggcgcacacc
     3901 tgcggtatcg atgatggtaa acggtttgcc ttcgcgctcg aaatcgatat ggatactgtc
     3961 gcgcgtcgta cctgccatat cgaaggtgat gacgcgctct tcgccgagaa tggcgttaac
     4021 cagcgtagat ttgccgacgt ttggacgacc gataacggca aaaacaggat gtcttgcatc
     4081 ggcttcttcg gcttccggct cggggaattt ttccaaaata tcttcaatca gataatacac
     4141 accatcgccg tgtgcacctg aaataacata agggtcgccc aaagcaagtt cgtagaactc
     4201 ggcggcaagt acagccctat tgcccccctc gcctttattc acggccaaat aaacagggcg
     4261 cggactttgg cgcaaacggt cggcaataat cttgtcttgc ggtgttaaac cggtacggcc
     4321 gtccaccaaa aacacaactg catcagcttc atcgacagcc tgtaaggttt gttttgccat
     4381 ttcgtgcaaa atgccgctgt ccacaaccgg ctcgaaaccg ccggtatcga tgaccaaata
     4441 aggtttgctg ccgactttgc cgtgtccgta atggcggtcg cgcgtcagac cgggcaggtc
     4501 atgcacgagc gcgtctttgg tgcgcgtcaa acggttgaat aaagtagatt tgccgacgtt
     4561 ggggcgacca acaagcgcga tggttggttt catgattgag tctttctgtg tcaagtgccg
     4621 ttcgggagaa ctgaacacga gcaggtgtcc gttggacacg gcggatgggt tttacgggaa
     4681 ttgccgtagg atagtgttgt ctgaaatgcc gtctgaagag agggtggcat ttcagacggc
     4741 atttatttca gcgaatcaag tttcatttga accaattcgc gaccgacaga atcttgaggc
     4801 attttttcta aagcctgtcc gtagtttttt aaggcttcct ggctttttcc ctgtgcggca
     4861 tagacatcgc ctttggtttc catcagcagg ggggcgaagt ccgcttcaac cggcgtatcg
     4921 agcgcggcaa gcgcggcatc gtattttttt tgttgcaaca acacaacgcc cagacgctgc
     4981 gccgccaacg cttgaatcag gctgtctttt tggttggaca acacccattt caaatggcct
     5041 tcggcaacat cgtaacgctg cgcgtcaaat tcggttgccg ccgccatcag tgtggcttgg
     5101 gcggcggaaa tggaatgcgg gtagctttgt tggagtttgg tcaattcggc attgatttcg
     5161 ctttgcgggg ctttgctttg cgccttttct acgatgtttg ccagcaccgc cgccgcttcc
     5221 tgattttggg aaactttacg gttttggtaa accgtgtatc ccaagtagcc gagtgccgcc
     5281 aaaatcagca aggcaaacag ccatttgccc gtggttttcc aaaaatattt aaagttgtct
     5341 aactcttgtt gttcttcgag atgggctgcc atttatgcgt tcttccattg ttgtaaagta
     5401 ggggttaaat cctcggcggc gacagtttgc tgaccgtgtg cgccgttcat gtctttgagc
     5461 gtaaccgtac cgttcgccag ttcgtcttgc gcgacaatca gggcaaagcg tgcgccgctg
     5521 ttgtcggctt ttttcatttg cgctttcagg ctttgatagc cggaatgctg cattacattg
     5581 aaaccttgcg cgcgcaaggc ttgtgcgtat ttcatcacct gcaagtccgc cccttcgcct
     5641 tggtgcattg catagacatc aggcgcagcg ttcacttcca gagagccgta ttcgctcacc
     5701 aaaagcagca gccgctcgat acccattgca aagccgatag acggcgcagg tttgccgccg
     5761 agttcttcaa tcaagccatc gtaacggccg ccgccgcaca cagtcgcctg cgcgccgagt
     5821 ttgtcggtcg tccactcaaa aaccgtctga ttgtaataat ccaaaccgcg aaccaagcgc
     5881 ggattttcaa tatattggat acccaaacca tccaacatcg ccttgaagcg tacatagtgg
     5941 ttttgcgaat cctcgcccaa gtaatccacc aaacgcggcg ccgcgttgca gatttcctgc
     6001 aaatctgggt ttttcgtatc caaaacgcgc aaaggattgg ttttcagacg gcgtttgctg
     6061 tcttcatcca atttatcttc ataacgggtc agatattcaa ccaatgccgc acggtgtgcc
     6121 gcgcgttcct cacggttgcc caagctgttg atttccaaag tcaggtattc gcggataccc
     6181 aatttttccc ataagtcggc agacatcgcg atgatttccg catcaatatc cggcccttca
     6241 aaacccaaag cctcgatacc gacctgatgg aactgacgat aacggccttt ttgcggacgc
     6301 tcgcggcgga acatcggccc catataccac agcttttgcg ggctattgta cagaaggttg
     6361 tgttcgacca ccgcacgcag gcaggaagcc gtaccttcag gacgcaagct caaactcaaa
     6421 gaatcgtttg aatcggagaa ggtgtacatt tccttgccga ccacatcggt ttcctcgccg
     6481 atggagcgga caaacaaacc ggtttgctcg acaatcggcg tacggatttg ctgataaccg
     6541 taagcgcgtg tccagcggcc gaccgtatct tcaaacgcct gccaaaacgc agccgtcagt
     6601 ttgaaatctt tttgcttgac aggcagaagg tcgttcatgc ctttgacgga ttggattttt
     6661 tgtgccattt caagtaagaa tgcttaaatc aaattgcggg cgattatagc ggattttaaa
     6721 gggtttgtga ggttggaggt ggtttgcgga cggcatttga cttactctgc acgtgcttgc
     6781 ctgatttgtc cgactgtaaa ctccgtctgc cgttttgggt gttgtgtgaa aaacaattta
     6841 tttgaaattg tctcggcttt tttcggtatg acagccaaaa tcttacctgc caaatttccc
     6901 tcacgggttt gccaagcatc caaaaactgc gccctgctca ttgaaacatg ccccagcgac
     6961 gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgaaaa atggtcgtct
     7021 ttgcggtatt tcagatacac gatgacgggg attttcaact gcgcgagctg ctcgaaagac
     7081 agggcatagc ccttcgcctc aaaacccaaa tcaggcataa tgcgccgcat atcctcaaac
     7141 gacgcgcgca tctgctcctt atccagtttt tccaacactt cttcttccgt cagcttttgc
     7201 ccgtaaaaat tgttcaaaag tgtcgccacc gaagccgccc cgcaggaaaa atccaaatcc
     7261 tgctttacaa tattgaaatc ccgcctttct ttccaactct gcactttgat ttttccgtaa
     7321 acaacaggat tatcgttaaa catcggtgca gcattcaaac gataagataa agaaacgaca
     7381 acacacgcca acagaaaaac atatttgaac ttcatcatat tgtccacata aagggcagcc
     7441 tgaaaatctt tcaggctgcc cttgtcaaat tattcctagc tttcggcttt tttggcaaac
     7501 caaacaatcc gattacccgc ataatacttt ccatttattg aaatccgaca agccgcgccc
     7561 aaaaaatgcc atgcactgtc gatttccgca gcaatctttg taccgttttc ttcaaattcc
     7621 aaatattcac ccaataataa acttgaaaca gaacgcgtgg gcagcaagtg cccaccctac
     7681 gcttattcaa ataatttgat taaataaaga ataaagaaaa tgcatcagga acaaaattat
     7741 aatctgccac ctgactcaca ccgcttttaa agtaaggggc atcaaaatca aaaccgcaaa
     7801 aaaaataatt tttgcattga tttttaatag atttaaaatt cgaatatagt gtttctccag
     7861 atttaaaaag tttatcttta tctatatatt tgatgctttc cctatccaaa ataaatattt
     7921 caaacattaa aaaatcatta catgaccaag ccaaatcata aatttgattc aaatgggtat
     7981 cataaagata aaaataataa ggtttgggaa caggtaaaat atttaatgga aaaggaggac
     8041 taattttctt aatatctgat gactgatatc catatctttc tatattctta aaaatttcat
     8101 ctttcttaag ataacaagac attctgacaa tcacaacctg ttcatcggat atattatcta
     8161 tttgcatggc gcgtataaca cgccatgcct gattaaaatt agtctccctt accttagaat
     8221 ctattatctt tttcaaagat gaaaatgccc cttatcttga actctccagt tagaacctgg
     8281 aagtttcgta cctcttaaat gtgtattaat atttcttata gtttcattaa aatgccacgc
     8341 gctgccgatt tcaacggtaa ttttcgtacc gctttcttca aattccaggt attcccccat
     8401 tagctaacgc aaagaagcag acgccatttc ggcttcgtta tgataaaccc gccttccgtt
     8461 gatatagact tccgcccttg tccgctccaa attccaaaaa ttccgtactg aaatttccat
     8521 atcccgatat tgtgcagacc atgttttttc gaaggttttc ataaaatttc ctatacctgt
     8581 ccaatcggca catatcaatt gcattattat agtggattaa caaaaatcag gacaaggcaa
     8641 cgaagccgca gacagtacaa atagtacaga accgatttac ttggtgtttc agcaccttag
     8701 agaatcgttc tctttgagct aaggtgagtc aacgccgtac tggtttaaat ttaatccgct
     8761 atacatctca atacgataaa tattccttaa gtcaaaatgc aagcctgacc gtaccttaac
     8821 tgtcaaaatt ttattatttt ttattgattt taaaacaatt tctgcaaaat tctcttcgct
     8881 ttctctcttt tttagaagca cataagaaaa aataaaactt ccccgattaa attcataaat
     8941 atgtttcaac cattcgcctc ctctttctgt aagacaagat tcagtttcat tcttccttat
     9001 tgtataaata tttccttcac aaaatctgaa ataaatccat aaatccatct tatccataat
     9061 taaagaaaaa gtttcacctc gagattttgt caacaattcg caaggttgcg atgttgcaat
     9121 caaatagccg aaagacattt tttacctcat acatggtcga aatcagtttc tgttagttca
     9181 gaatccattt tttcgtcaac aactgaatcc gcatttttga attaacgttt tcatcagctg
     9241 ccgtttatct aaaccggcag gttcagtttc aaaataagcc ttatatgaag actgtaagca
     9301 tttcagaaaa agatcatcag aagacatatc tgccgaatca aatacaactg ttttgatttt
     9361 ggtacttacc caaaaccctt tttgctcttt ttctactata cggaaattca gaatatttcc
     9421 aaccgaatca aaagcacggt aaacatcatc catcaaatcc tgcggctcta ttttcttttc
     9481 caattccgac aatccttgaa atatatccaa agacacatct tcaaatagaa aaaaaggagg
     9541 agttagaagc ggtttttcca tgatctgtcc gtagattttg attcccaagg gcgatgacga
     9601 ccaattccct gtccaggcaa agtcttgccc gtattatccg taactcgacg atgataatgg
     9661 ggaaattttc caataggatg acctgttcta ttaccgaaag gggctatccg catattattg
     9721 ccgattttaa tctcacgtcc atatttagca aaggaaacaa cctttcctgc ggcgcctaca
     9781 ccaccaggaa ttgcgcctaa tccgccagca atagcaacat ctctaacaga agctggtctg
     9841 cctgtcgttg cataactaaa accatgctgt gtccacatac caatggcagc accacccaag
     9901 atagccaatg gaagaaacgc cccctcagtc tccttcatct ccttctgaga aagctccgcc
     9961 aactgcatcg gcgcatctgc ccgcgtatgg aatacttggt tttcaaatat ctgattgtcc
    10021 atgctgtttg ccatcgccgg agcggcaatc attgtcagaa tcatcatcgc tgcggtaatt
    10081 tgttttttca taataactcc tttggattac aaggttggaa aatcaaagcc ctgcttagaa
    10141 cgtatgttgc acacccaatt tcaataggta aatcagattg caaatccagc aatttgaata
    10201 ttgtcattgt tccgtgcaaa agggattttt attgatgagt tgtgtactgg gtttcagctt
    10261 ggctttttag ataatctttt cacaagaaca tacatcaata ctgtccaaaa aaaaagattt
    10321 gataaatata ttgcaacata ttttattcca ccttcatccg aaacagaaac caatatattt
    10381 ttaattaata catagctgat tattatattt attattccta taatatataa ggatcttgcc
    10441 aaaatttttt ttgattttat tttaggaata tctcttatcc atgctaaaat acagcccaat
    10501 gtcgaaaaga aaataacagc aaacactacc ttacctcctc tcacttccaa ccaaaccgat
    10561 agcaggtttg gttggaagtt ggtttattta ttatttaacg gcgaatgtca gtgttcttac
    10621 ccgtagaacc tgcataacca tttatgccac cagcaacagt ccccactgct ac
//
LOCUS       DAWIVB010000053        10656 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWIVB010000053 DAWIVB010000000
VERSION     DAWIVB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.053"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..2034)
                     /locus_tag="WIX60_000974"
     CDS             complement(<1..2034)
                     /locus_tag="WIX60_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ5305257.1"
                     /translation="MPAGRLPRRCPMMTKFTDCTRSNRIQPPTHRGYILKNNRQIKLI
                     AASVAVAASFQAHAGLGGLNIQSNLDEPFSGSITVTGEEAKALLGGGSVTVSEKGLTA
                     KVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQVREYTAILDPVGYSPKTKSALSDGK
                     THRKTAPTAESQENQNAKALRKTDKKDSANAAVKPAYNGKTHTVRKGETVKQIAAAIR
                     PKHLTLEQVADALLKANPNVSAHGRLRAGSVLHIPNLNRIKAEQPKPQTAKPKAETAS
                     MPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAVRPEPVPAANTAASETAAESAPQEA
                     AASAIDTPTDETGNAVSEPVEQVSAEEETESGLFDGLFGGSYTLLLAGGGAALIALLL
                     LLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAEVETPATPEPAPKNDVNDTLA
                     LDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQNGILSGALTQDEETQKRADAD
                     WNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTAENKPETVDTDFSDNLPSNNH
                     IGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQTPEELHDFLKVYETDAVAET
                     APETPDFNAAADDLSALLQPAEAPSVEENITETVAETPDFNATADDLSALLQPS"
     gene            2240..2770
                     /locus_tag="WIX60_000975"
     CDS             2240..2770
                     /locus_tag="WIX60_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ5305258.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            2773..3063
                     /locus_tag="WIX60_000976"
     CDS             2773..3063
                     /locus_tag="WIX60_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ5305259.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            3063..3341
                     /locus_tag="WIX60_000977"
     CDS             3063..3341
                     /locus_tag="WIX60_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ5305260.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            3390..4256
                     /locus_tag="WIX60_000978"
     CDS             3390..4256
                     /locus_tag="WIX60_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5305261.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRTVG
                     ALLGKANIKPAK"
     gene            4327..5085
                     /locus_tag="WIX60_000979"
     CDS             4327..5085
                     /locus_tag="WIX60_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5305262.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSEDELHKFYEQQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            5158..5649
                     /locus_tag="WIX60_000980"
     CDS             5158..5649
                     /locus_tag="WIX60_000980"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ5305263.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            5680..6690
                     /gene="dusA"
                     /locus_tag="WIX60_000981"
     CDS             5680..6690
                     /gene="dusA"
                     /locus_tag="WIX60_000981"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ5305264.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANM
                     RE"
     gene            complement(6760..7647)
                     /gene="gluQRS"
                     /locus_tag="WIX60_000982"
     CDS             complement(6760..7647)
                     /gene="gluQRS"
                     /locus_tag="WIX60_000982"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ5305265.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGKWLIRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPALQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAVTAP"
     gene            complement(7664..7861)
                     /locus_tag="WIX60_000983"
     CDS             complement(7664..7861)
                     /locus_tag="WIX60_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305266.1"
                     /translation="MTKLVSRNASNERIADRAFDFFSPALMDGSATEEQYNALYDLTL
                     LEEPGMELNKDEIMALINSLK"
     gene            complement(7935..8294)
                     /locus_tag="WIX60_000984"
     CDS             complement(7935..8294)
                     /locus_tag="WIX60_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ5305267.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     gene            complement(8538..9593)
                     /gene="tal"
                     /locus_tag="WIX60_000985"
     CDS             complement(8538..9593)
                     /gene="tal"
                     /locus_tag="WIX60_000985"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ5305268.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETKGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLA"
     gene            9690..>10656
                     /locus_tag="WIX60_000986"
     CDS             9690..>10656
                     /locus_tag="WIX60_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ5305269.1"
                     /translation="MAENGKYLDWAREVLHAEAEGLREIAAELDKNFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
BASE COUNT         2336 a         2658 c         3107 g         2555 t
ORIGIN      
        1 agaaggttga agtaatgcgg acaaatcgtc tgcggtggcg ttgaagtcgg gtgtttcggc
       61 aacggtttcc gttatatttt cctcaacgga cggtgcttcg gcaggttgaa gcaatgcgga
      121 caaatcgtct gcggcggcgt tgaaatcggg cgtttcaggc gcagtttccg cgacggcatc
      181 ggtttcgtac actttcagga aatcgtgcaa ctcttccggt gtttggactt cggcaactgt
      241 tttttccaag atggtttcgg gcgaggaagc cttcaggaag cctgccagtc cggagggtga
      301 ggcaggtttt gcggaagctg tttcttctgt gccgatatgg ttgtttgagg gcaggttgtc
      361 ggagaaatcg gtatcgacgg tttccggttt gttttcggca gtttgggcga cagattccgg
      421 ttcgggcgtg tcgatgacga tttcgacagg gttgtacggg ttgaaggtct cgggctcgta
      481 cacgctgtct gtggattcga tggcgttcca atcggcatcc gcgcgttttt gggtttcttc
      541 atcctgcgta agtgcgccgg ataaaatgcc gttttgcgcg gctgccaggc tgtcgaaatc
      601 caagtcgatg cggttggaag gcgtatcggt ttcgacatcg aacgtttgtt ttgccgataa
      661 ctcttcttca gattccccat ctaaggcaag tgtgtcgttt acatcgtttt tcggagcggg
      721 ttcgggcgtt gccggagttt cgacttcggc aaaggtgatt tctatgccgt cgtctgccgc
      781 gtcgtcaagg tcaggctctt cctcagggac ggattcttcg gtacggcgcg cgcgtttgga
      841 ttgggcaagg cgcaaaagca gcagcagggc gattaatgcc gcgcctccgc cggcaagcag
      901 caaggtgtac gaaccgccga acagaccgtc aaacagtccg ctttcggttt cttcttcggc
      961 agaaacctgt tcgacaggtt cggaaacggc gttaccggtt tcgtcggtcg gcgtgtcgat
     1021 ggcagaagcg gcggcttctt ggggggcgga ttcggcagcg gtttccgatg cggcagtatt
     1081 tgcagcgggt acaggttcgg gtcgaacggc cggtttttcc gcttttgctt cgggcgcggc
     1141 aacttttgct tcaggttttt caaccggttt ctctaccgtt gcctgtttgg acggttcgga
     1201 cggcatggat gcggtttcgg ctttgggttt cgccgtttgc ggtttgggtt gttccgcttt
     1261 gatcctgttc agattcggaa tgtgaagcac gctgcccgca cgcagtctgc cgtgtgcgga
     1321 aacatttggg tttgccttca gcagcgcatc ggcaacctgt tcgagcgtca ggtgtttcgg
     1381 gcggatggcg gcggcaatct gtttgaccgt ttcgcctttg cggacggtat gggttttgcc
     1441 gttgtacgcc ggtttgacgg ctgcgttcgc gctgtctttt ttatcggttt tgcggagggc
     1501 tttggcgttt tgattttctt gggactctgc tgtcggagcg gttttgcggt gtgtcttgcc
     1561 gtctgaaagt gcagatttgg ttttgggcga gtagccgaca ggatcgagga tggcggtgta
     1621 ttcgcgtacc tgtgcgcctg cgccgatgcg gaacaccagg acgggatcgc ggactgcctg
     1681 ttcggaagaa acggcaatga cggctttgtc gcccaacttg tggactttgg cggtcaggcc
     1741 tttttcggaa acggtaacgc tgccgccgcc tagcagggct ttggcttctt cgccggttac
     1801 ggtaatgctg ccggaaaagg gttcgtcaag gttggactgg atattcagtc cgcccagtcc
     1861 agcatgtgcc tgaaaggatg cggcaactgc gacggaggcg gcaatcagtt tgatttgtct
     1921 gttgtttttc aagatgtatc ccctgtgggt tggcggctga atacggtttg accgcgtaca
     1981 gtctgtaaat ttcgtcatca tcgggcatcg gcggggcagt cggccggcgg gcatttaata
     2041 tgtgaatgta ccgaccgccg ccacatttta aacggcaatc attcgccgtt tttacaaatt
     2101 atgacatatc tccatctttt ttcaaaaaca tctgtgcata tttgcatcaa tcaaaacaaa
     2161 atttgttggt tttgcaggtg caaaaacagg gttctgcctg tatgattagc gtttatttga
     2221 tttgctttct catttggata tgaaattcgt cagcgacctt ttgtccgtca tcctgttttt
     2281 cgccacctat accgttacca aaaacatgat tgccgcaacg gcggtcgcat tggttgccgg
     2341 tgtggttcag gcggcttttc tgtattggaa atataaaaag ctggatacga tgcagtgggt
     2401 cggattggtg ctgattgtgg tattcggcgg cgcaaccatt gttttgggcg acagccgctt
     2461 cattatgtgg aagccgagcg ttttgttttg gctgggcgcg ctgttcctgt ggggcagcca
     2521 cctcgccggt aaaaacggct tgaaggcgag tatcggcagg gagattcagc ttccggatgc
     2581 cgtatgggcg aaattgacgt atatgtgggt cggtttcctg atttttatgg gtatcgccaa
     2641 ctggtttgtg tttacccggt tcgagtcgca atgggtcaac tataaaatgt tcggctcgac
     2701 tgcactgatg cttgttttct ttattattca gggtatttat ctgagtacct gtctgaaaaa
     2761 ggaggattga ctgtggaata ttttatgttg ctggcaacag acggggagga tgtgcacgag
     2821 gcgcgtatgg cggcacgtcc cgaacacctc aaacggctgg agacgctgaa gtcggaaggc
     2881 cggctgttga cggcaggccc gaatcctttg ccggaggact ccaaccgcgt ttcgggcagt
     2941 ttgattgtgg cgcagttcga gtctttggat gcggcgcagg cttgggcgga agacgatccc
     3001 tatgttcatg caggcgtgta cagcgaagtg ctgatcaagc cgtttaaagc ggtgttcaaa
     3061 taatgccggc cgtcgatttg atccgcgaac gcctgcagac gctcgatccg ctggtgttgg
     3121 aaatcggcga tgagagccat ctgcacaaag gacacgcggg caataccggc ggcggacatt
     3181 atgccgtttt ggtcgttagc ggccgttttg aaggcgtaag ccgcctgaac cgccagaaaa
     3241 cggtcaaatc gctgctcaaa gatttgtttt caggcggcat gattcacgcg ctcggcatcc
     3301 gggcggctac ccctgacgag tatttccata cggcggactg aatgaagtct gcccgaacat
     3361 ttcaatttaa aatttaaaga gagaagatta tgaaagcaaa aatcctgact tccgttgcac
     3421 tgcttgcctg ttccggcagc ctgtttgccc aaacgctggc aaccgtcaac ggtcagaaaa
     3481 tcgacagttc cgtcatcgat gcgcaggttg ccgcattccg tgcggaaaac agccgtgccg
     3541 aagacacgcc gcaactgcgc caatccctgc tggaaaacga agtggtcaat accgtggtcg
     3601 cacaggaagt gaaacgcctg aaactcgacc ggtcggcaga gtttaaaaat gcgcttgcca
     3661 aattgcgtgc cgaagcgaaa aagtcgggcg acgacaagaa accgtccttc aaaaccgttt
     3721 ggcaggcggt aaaatatggc ttgaacggcg aggcatacgc attgcatatc gccaaaaccc
     3781 aaccggtttc cgagcaggaa gtaaaagccg catatgacaa tatcagcggt ttttacaaag
     3841 gtacgcagga agtccagttg ggcgaaatcc tgaccgacaa ggaagaaaat gcaaaaaaag
     3901 cggttgccga cttgaaggcg aaaaaaggtt tcgatgccgt cttgaaacaa tattccctca
     3961 acgaccgtac caaacagacc ggtgcgccgg tcggatatgt gccgctgaaa gatttggaac
     4021 agggtgttcc gccgctttat caggcaatta aggacttgaa aaaaggcgaa tttacggcaa
     4081 cgccgctgaa aaacggcgat ttctacggcg tttattatgt caacgacagc cgcgaggtaa
     4141 aagtgccttc ttttgatgaa atgaaaggac agattgcggg caaccttcag gcggaacgga
     4201 ttgaccgtac cgtcggtgca ctgttgggca aggcaaacat caaacctgca aaataattct
     4261 gaaaacggga tatggcggca agacgttcag acaggcgttt tgccgccgcg caggacaggg
     4321 aataccatga aacagaaaaa aaccgctgcc gcagttattg ctgcaatgtt ggcaggtttt
     4381 gcggcagcca aagcacccga aatcgacccg gctttggtgg atacgctggt ggcgcagatc
     4441 atgcagcagg cagaccggca tgcggagcag tcccaaaaac cggacgggca ggcaatccga
     4501 aacgatgccg tccgccggct acaaactttg gaagttttga aaaacagggc attgaaggaa
     4561 ggtttggata aggataagga tgtccaaaac cgctttaaaa tcgccgaagc gtctttttat
     4621 gccgaggagt acgtccgttt tctggaacgt tcggaaacgg tttccgaaga cgagctgcac
     4681 aagttttacg aacagcaaat ccgcatgatc aaattgcagc aggtcagctt cgcaaccgaa
     4741 gaggaggcgc gtcaggcgca gcagctcctg ctcaaagggc tgtcttttga agggctgatg
     4801 aagcgttatc cgaacgacga gcaggctttt gacggtttca ttatggcgca gcagcttccc
     4861 gagccgctgg cttcgcagtt tgccgcgatg aatcggggcg acgttacccg cgatccggtc
     4921 aaattgggcg aacgctatta tctgttcaaa ctcagcgagg tcgggaaaaa ccccgacgcg
     4981 cagcctttcg agttggtcag aaaccagttg gagcagggtt tgagacagga aaaagcccgc
     5041 ttgaaaatcg atgccctttt ggaagaaaac ggtgtcaaac cgtaatggca tttccaatac
     5101 cgatgccgtc tgaagccttt cagacggcat tgcacgttca ggtaaggagg acggcttatg
     5161 cgtgcggtca tacagaaaac ggtaggtgca aaggtggatg tcgtgtccga agccggcacg
     5221 gaaacctgtg gcaaaatcga cggcgggttt gtcgtgttac tcggcgtaac gcatagcgac
     5281 acagaaaaag atgcacgcta tatcgccgac aaaatcgccc atttgcgcgt gtttgaagac
     5341 gaagcgggca agctgaacct gtctttgaaa gatgtcggcg gcgcggtgct gctggtgtcg
     5401 cagtttacgc tttatgccga cgcggcaagc gggcggcggc cttcgttttc ccaagccgca
     5461 cctgcagaac aggcgcagca gctttacctg cgaacggcgg aactgttgcg cggacacggg
     5521 attcatgtcg aaacagggcg tttccgcacg catatgcagg tgtcgctctg caacgatggg
     5581 ccggtaacca tactgctgga ctctttcatg acgcggattt ccccaaaaat gaaggttgtt
     5641 ccggattgaa attgaatccg caatgataaa atatcgacaa tgaacgacaa tacacacacc
     5701 cttcccccgc gccacctgtc cgtcgccccc atgctcgact ggacggacag gcactaccgt
     5761 taccttgccc gccagattac ccgaaatact tggctgtaca gcgaaatggt caatgccggt
     5821 gcgattgttt acggcgacaa agaccgcttt ttgatgttca acgaaggcga gcagcccgtc
     5881 gccctgcaac tgggcggcag cgatccgtcc gatttggcga aagccgccaa agccgccgag
     5941 gcatacggtt acaacgaggt caacctcaac tgcggctgcc ccagtccgcg cgtgcagaaa
     6001 ggctcgttcg gcgcgtgtct gatgaacgaa gtcgggctgg ttgccgactg cctcaacgcc
     6061 atgcaggatg cggtcaagat tcccgttacc gtcaaacacc gcatcggtgt ggacaggcag
     6121 accgaatacc aaaccgttgc cgatttcgtc ggcacgctgc gcgacaaaac cgcctgcaaa
     6181 acctttatcg tccacgcccg caacgcttgg ctggacggtc tttcccccaa agaaaaccgc
     6241 gacgttcccc cgttgaaata cgattacgtt taccgcctca agcaggagtt tcccgggctg
     6301 gaaatcatca tcaacggcgg catcaccacc aacgaagcaa tcgcaggaca cctgcaacac
     6361 gttgacggcg tgatggtcgg gcgcgaggcg taccacaacc cgatggtgat gcgcgaatgg
     6421 gacaggctgt tttacggcga tacccgcagc ccgattgaat acgccgattt ggtgcagcgt
     6481 ctctacacat acagccaagc ccaaatccaa gccggacgcg gcacaatctt gcgtcacatc
     6541 gtccgccaca gccttgggct gatgcacggt ctgaaaggcg cgcggacttg gcggcgtatg
     6601 ctttccgacg caacgctctt gaaagacaac gacggcagcc tgattctcga agcgtggaaa
     6661 gaggtcgaac gggcaaatat gcgcgaatag ggcggggctg tatgtgtgaa atgccgtctg
     6721 aaggcttcag acggcatttg tgcgtttgtc gggcggtgtt tagggggcgg taacggcgtg
     6781 tttcggcact ttgtccatat cccagtgtgc caccgcccag tcgagcagtt cggcagggcg
     6841 gtcggtttcc ggtgcttcgg gcagcttgag gtaacggaac acttggcgga ggagttgttc
     6901 gcggcggttt aaatccaatg cgggggcgag cgtctgtttc gaccatttct gcccttgtgc
     6961 gttggtcagc agcggcaggt gggcatattg cggtgtcgga acgtccaaac actgctgcaa
     7021 atagatttgg cgcggcgtgg aaacgagcag gtcttgtccg cggacgatgt gggtaacgcc
     7081 ctgttcggca tcgtcggcaa cgacggcgag ctggtatgcc cagtaaccgt ctgcacgaag
     7141 caggacgaaa tcgccgatgt cgcgggcgag gttttgggcg taaccgccga cgatgccgtc
     7201 tgaaaaaccg ataatgcggt cggggacgcg gatgcgccac gccggctgtt tgccttgcag
     7261 tgcagggcgt tggccggggt ggcggcaacg tccgttatag acgaacccgt ctgcgccccg
     7321 ccttgccccg gcctgccagt ctttgcggct gcaatggcag ggatagacca gtccggcggt
     7381 tttcaggcgg catagggttt cttcatacag ggcgtaacgg cggctctgat aggcgacttc
     7441 tccgtcccac tcgaatccga atgcctcaag cgtgtgcagg atatggcttg ccgcccccgg
     7501 catttcgcgc ggcggatcga ggtcttccat gcggatcagc catttgccgc cgtgcgcgcg
     7561 cgcatcggca taggaagcga cggcggtcag cagcgagccg atgtggagca gcccggtcgg
     7621 gctgggggca aaacgtcctg tgtacatatc tggtacagcc cctttattta agactattaa
     7681 tcaaagccat tatctcatct ttattcagtt ccatcccggg ctcttcaagc aaggttaaat
     7741 catatagggc attatattgc tcttcggtag ctgaaccatc cataagagca ggcgagaaaa
     7801 aatcaaaggc tctatctgca attctctcat tacttgcatt tctactaacc agtttcgtca
     7861 attctgtata ttttgaaaag tttatggaaa aataaaacag cgaaaaagtt ttggtttcgc
     7921 tgtttttgat ttaattagca ctgataatct tcaaattccc acgaaaaaaa acgaagtaaa
     7981 taagtcaatg acttttccca agtttctttt gaacattctt taagaatttt ctcaatttcc
     8041 gatttaataa cagaatgatt aaattcattc ataatcatca tacccgcccc ccatttaacc
     8101 ctttgatttt ggaaacaatt atgcaaaatc catttaggag agcatatgcg aacagaaaat
     8161 atatctgcag catcactatc atcagttcct atgtctaaat caattcccac acaaaaattg
     8221 tctttgattt cgggaacgaa atcttcaaag gcacaatcgt aaagattgat ggctttcaat
     8281 tctaggttaa tcattttata ttcaatagta tggggaggta ccggatcctt aaaaatcaga
     8341 tctgaataaa tttcattggg tgaaatgatt tcgattgctt ttgccatgat tctatttcct
     8401 tttgtgttag tgggtaatgt cgtgcattaa cttcttgccc attaatattt ttagggtgaa
     8461 tccttgatat gccgcactgt gtccggtcaa acgggcgatg ccgtctgaaa gcctttcaga
     8521 cggcatcggg aaaatgccta agccaaaggc gcgagcagtt tttcaaacgc ttcttcaaac
     8581 tgtttcaaac cgtcttcctg caaacgcgtt gccaaggttt cgacatcgat gccgagcgcg
     8641 gcggtttcgg cgagctgcgc ttgtgcttct tctacgcctt cggtcagcgt ggctttggct
     8701 gtgccgtggt cgataaaggc tttgagcgtg gcatcgggaa cggtgttgac ggtgtgcgcg
     8761 ccgatcaggc tgtcaacgta gagcgtgtcg ggataggccg ggtttttcac gccggtagat
     8821 gcccataaaa gctgcacgcg gtttgcgcct ttggtttcca gcgcggcaaa ttcggggctg
     8881 ccgaagtatt gcgcccagtc ttggtaggcg gctttggcaa gggcgatggc gattttgcct
     8941 ttgaggtggt cgggcagtgt tgtgtccagc gcgccgtcca cacgggagat gaagaagctg
     9001 gcgacaactt ggatatgggc aacgctttgt ccggctgcta agcgtttggc gatgccgcgc
     9061 gcgtaggcgg cgtaggcttt gagggtttgg gcgcgtgaga acagcagggt caggttcacg
     9121 ctgatgccgt ctgaaacgag ggtttcgagc gcatcgatgc ctgcgtcggt ggcaggcact
     9181 ttaatcatcg cgtttttgca cccgatggcg gcgtagaggc ggcgcgcttc ttcaaccgtg
     9241 ccttgcgcgt ctttggacaa ttcgggcgaa acttcgaggc tgacgaagcc ggttttgccg
     9301 ccggtggatt cgtgttcggc aaggcaaacg tcgcaggcgg cacgcacatc ggcaaccgcc
     9361 attgtttcgt agcgttgttt ggggctgagg ttttgctgct tgagggcggc gatttcatcg
     9421 gcgtaaagcg cgtcgccggc gaaggctttt tggaagatgg cgggattgga agttacgccg
     9481 cacacgccct gtttcaacat ttgcgccaat tcgccgcttt gcactagcga gcgggaaagg
     9541 ttgtccagcc agatttgttg tcctaatgct ttaacgtccg ataaaatggt catctctgat
     9601 tcctttggat ggataggcgg ggtttgaggg cttatgctac cccgattcgg aaattttggg
     9661 tagttttatt acagcaaagg cggatggcaa tggcagaaaa cggaaaatat ctcgactggg
     9721 cacgcgaagt gttgcacgcc gaagcggaag gcttgcgcga aattgcagcg gaattggaca
     9781 aaaacttcgt ccttgcggca gacgcgttgt tgcactgcaa gggcagggtc gttatcacgg
     9841 gcatgggcaa gtcgggacat atcgggcgca aaatggcggc aactatggcc tcgaccggca
     9901 cgcctgcgtt tttcgtccac cctgcggaag cggcacacgg cgatttgggt atgattgtgg
     9961 acaacgacgt ggtcgtcgcg atttccaatt ccggcgaaag cgacgaaatc gccgccatca
    10021 tccccgcact caaacgcaaa gacatcacgc ttgtctgcat caccgcccgc cccgattcaa
    10081 ccatggcgcg ccatgccgac atccacatca cggcgtcggt ttccaaagaa gcctgcccgc
    10141 tggggcttgc cccgaccacc agcaccaccg ccgtcatggc tttgggcgat gcgttggcgg
    10201 tcgtcctgct gcgcgcacgc gcgttcacgc ccgacgattt cgccttgagc catcctgccg
    10261 gcagcctcgg caaacgccta cttttgcgcg ttgccgacat tatgcacaaa ggcggcggcc
    10321 tgcctgccgt ccgactcggc acgcccttga aagaagccat cgtcagcatg agtgaaaaag
    10381 ggctgggcat gttggcggta acggacgggc aaggccgtct gaaaggcgta ttcaccgacg
    10441 gcgatttgcg ccgcctgttt caagaatgcg acaattttac cggtctttcg atagacgaag
    10501 tcatgcatac gcatcctaaa accatctccg ccgaacgtct cgccaccgaa gccctgaaag
    10561 tcatgcaggc aaaccatgtg aacgggcttc tggttaccga tgcagatggc gtgctgatcg
    10621 gcgcgctgaa tatgcacgac ctgctggcgg cacgga
//
LOCUS       DAWIVB010000054        10599 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWIVB010000054 DAWIVB010000000
VERSION     DAWIVB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.054"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..290)
                     /locus_tag="WIX60_000987"
     CDS             complement(<1..290)
                     /locus_tag="WIX60_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ5305270.1"
                     /translation="MDKELNPSIGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILH"
     gene            complement(391..1059)
                     /locus_tag="WIX60_000988"
     CDS             complement(391..1059)
                     /locus_tag="WIX60_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ5305271.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(1056..2201)
                     /locus_tag="WIX60_000989"
     CDS             complement(1056..2201)
                     /locus_tag="WIX60_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ5305272.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALGKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGLRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(2191..3522)
                     /locus_tag="WIX60_000990"
     CDS             complement(2191..3522)
                     /locus_tag="WIX60_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ5305273.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIANIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(3522..5693)
                     /locus_tag="WIX60_000991"
     CDS             complement(3522..5693)
                     /locus_tag="WIX60_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ5305274.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTGLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLS
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPDTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALGWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            5848..6450
                     /locus_tag="WIX60_000992"
     CDS             5848..6450
                     /locus_tag="WIX60_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305275.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLFTALTEVV
                     KESNQTGATAQKDVPADGILYSVVFEKETNTIAIIGRKQP"
     gene            complement(6494..6727)
                     /locus_tag="WIX60_000993"
     CDS             complement(6494..6727)
                     /locus_tag="WIX60_000993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305276.1"
                     /translation="MRRRRRSSKTQLPDGTQKLPSGGHPDGKAGIGADAVRDMCHAEL
                     SAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(6702..7067)
                     /locus_tag="WIX60_000994"
     CDS             complement(6702..7067)
                     /locus_tag="WIX60_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305277.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     RLKPSAATASPDAPSAAEQ"
     gene            complement(7071..7448)
                     /locus_tag="WIX60_000995"
     CDS             complement(7071..7448)
                     /locus_tag="WIX60_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ5305278.1"
                     /translation="MSDATYADAVIMEMNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEDRRTSYLDCFTVDTG
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(7513..8922)
                     /locus_tag="WIX60_000996"
     CDS             complement(7513..8922)
                     /locus_tag="WIX60_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ5305279.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVADRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANAFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVSNAIEQRGCIGVLGMSAALSTATTATGEINDGRM
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPLFAECNNA
                     LYNGLTPLTVVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(8925..9122)
                     /locus_tag="WIX60_000997"
     CDS             complement(8925..9122)
                     /locus_tag="WIX60_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ5305280.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(9119..9769)
                     /locus_tag="WIX60_000998"
     CDS             complement(9119..9769)
                     /locus_tag="WIX60_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ5305281.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(9759..10181)
                     /locus_tag="WIX60_000999"
     CDS             complement(9759..10181)
                     /locus_tag="WIX60_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ5305282.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(10181..10579)
                     /locus_tag="WIX60_001000"
     CDS             complement(10181..10579)
                     /locus_tag="WIX60_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305283.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
BASE COUNT         2164 a         3125 c         2712 g         2598 t
ORIGIN      
        1 atatgcagga tcaggcggcc gtcgtgcggc tgctctgcgc gcacggtaat cttgtcggca
       61 cgcccgctct taacaatcgg ttgcagtgcc tcatcggcat attgctcggc caacaggctg
      121 acccgttgca ggtctttttc gcgctgcaac aaatgcagca gcgagccgag cgttttatcc
      181 gcccaccagc tgcccaacgg tgtcatcaag cggatataca cggcattttg cagcgtatcg
      241 acggtgcggc cggtatagtc gccgatgctg gggtttagct ctttgtccat gttggcattt
      301 tgccgttttc agacggcctc acggagtaca cgggcttcaa tcccacacgc acaaaaaaag
      361 cagcccgaag gctgccaaag gtctgcttgt ctatgccggt tccgccggta aggttttgcc
      421 gccgatgctg tcggtatgcg ggtgctggcg caacgatata ttgccggcca ccacgtcgcc
      481 gtcggtgtta aagctgccgc ccgtttggtt aacatcgccg ctaaaggtgg ctccgtcgcc
      541 gccctcgacg gccatgccgc cgttgccgtt gatttggcct tgcgccgtca acactgcact
      601 ggtctccacc aacggagcgt taaatttggc atccgtggcc gcattaacct cgtattgttt
      661 gcagttaacc cggtacacgt cgcaatcggc ctcaatgatt ttgccttgct taatcacgat
      721 ttttgcaccc tcatgattaa aaatcgccgt ctcgccgggc ttaaggtttt tgatgcggta
      781 gctgccgtgc tggctgcaca caatcacacc gtgcgaagta ttgccgccca gcggtatcac
      841 taccgcttcg ctgccgtcgg gcggatggct ggcaaagccg tattcctgca aatgttcaag
      901 gtcttgcagg gtttcgtcgg ccaagccgct caactgcacg cgctgtatcg gctcggacga
      961 caccaccagc gtgatttttc cccgaaaggc cgcgcgcagg gtttcgccga tatttttagc
     1021 agtttgtgcc gtttttttcg ccaatttact caaactcatt caaacaccgc cgtttctgct
     1081 tgttttttgc cgcctttttt gcccttatgg ctcacgcctt tgcgtttgcc tttgcgcttg
     1141 cgcgccgcct cggccttttt ggggtaagcg tcgggtgtcc aaataccgtc ctctttgagc
     1201 cgcagctcgg tttgcgtacc atccatgcgg gatagcataa accgccgccc catcagaaaa
     1261 aacaccgcat cgataccgtg ctcgtcgtcg atcacatgca cacgcaggcc aggttgccac
     1321 aatacgccgt cgcgggtttt atggccgccc acggttatcg tgagtgtaaa tccctccagc
     1381 cgccagtcgg ccagctgctt tttagcctgc ttttgcaatg cggccaaatt gtcggcatcg
     1441 gacaccacca ccgttttagg gcggtgcagc gtcatcgtcg ggtctttgta cacccacttt
     1501 aaatcgtgtt tggcgctgtc gccgctgcgg ccgtgcgatt gcgccaaaaa agtaacctcg
     1561 gaaaagcggt tgtcggtatc ccactcaatg tccatgcgct cgatattgca gcggctgtcg
     1621 gtgcggctcc aacacaatgt cgccaccggc gggctgctgt aatccgcacc gcccaccacc
     1681 aacgtgccgt ccggctccag ccacggatgc agcccgaccg agttggcaat atgggttaat
     1741 gcctgccata cggtttcgcc cggctcgatg tcgattttgc ccaaagcggg gttgttttcg
     1801 gccttaagca ccaccgcttt aatctgcggc cacggcgcgg ccagcttttt ggctgcatcc
     1861 aataccgtca tgccctttac attgagctgc ggcgcggagc aatccaccaa aaaaccggcc
     1921 aaatcacgcc cgctcaagct caactcgcgg ctgcccttgc ttttgccgtg gcgctggctg
     1981 ccgatgatgc ccgtcatcac gatttgcccg tctatcacta cctcgcagct ctctccgctt
     2041 aaatcgggta tggccgcctc cggtcccaac ctgccgatga caaaatcgaa gctgtcggca
     2101 gggattaaaa agtcgctgtc gatgtcgtag cgctcccagt ggcggtgctc tttaccgccc
     2161 acgcgcaccg acacggcata gccgtatgaa ttattttgca tagctgttga ccaaagtgcc
     2221 gcgcttgata aacgcggggt ggtggatatg gggattgagc cgcaccagct ctgctgcgcg
     2281 ggctatatcg ccgtaaaact cgtgggcgat ttggtgtatc gtaccgtcga ttggggcttg
     2341 gcgcacaatc agcggcggct tttggttgat gaccgccgca accaacgcat tcagacggcc
     2401 tgccgccgcg cgcagggatt ctgccgtttg gtaagcctcg gtatacacgg cgttggccgt
     2461 cagcccacca gactcggcag cagccgtctg caccgcccgc aaggcggcaa tctcggcctg
     2521 catacggcgg cgcatggcgc ggttaacctc aatcaaatcg ggcgcggtca tctcttcgcc
     2581 atgcgcctcg attaatgccg ttgccaccga caacagcgat gacgtggata acaggcgcac
     2641 cgcctgcgct accggctgca cctgtttggc ggttaaccgg ttcaggcggt tttgcaggcc
     2701 gtctgaaaag cggccggtca gcaaattatc agggatagcg gcggcgcggt cggcaacagc
     2761 cgcagccccg tcaaaccgct gtcgcggcga ccaaccggca tggtgcatgg cattatcggc
     2821 caaacccgcc tcacggcgta tgccagtatc taccatgacc gatatatccg caaacagctt
     2881 ggccgagccg tttttaaacg ccgctgcact gtatccgccc cgatcgggaa aggcaatttt
     2941 gtccaaatca aacaaacggc gcaccgcgcc aaacgtaccc gatgccgcac tccaaatgcc
     3001 cagcgcgctg ccccataaag ctgatacgcc cgcatccacc gccaacaccg catcaacaaa
     3061 gccgatagcc gcctcgcggt aggtgtcgat attagcaatc aacgcctcaa gctcgaccaa
     3121 aaaggcgttt tcaaaaacaa agatttcctg cgcttcggcc gcctcgcgga aagtaatatc
     3181 gatgcccgca taatccacat aatcggcctc atgtcggtaa ctccatgatg ccgcaatcat
     3241 gttgtgcatc cgcccccaaa cagggtgcac cagcacgccg ccgcccggct gctccagcgc
     3301 atccagcagc tttttcagac ggcctgcata gcccttgccc caaaacaccg cattaatctg
     3361 cacctgccgc ccggtcatgc ccatgtcttc aaggtcgata ccctgcacaa acggccgcgc
     3421 atgctcggcc aatgccttgc cgttgctctc gtccaccacc tcaatatcaa agccgacgcc
     3481 cttgtaagat gcgtcctgca ataaggtatg ccatccgctc attattgacc cgctccacgg
     3541 ccgaacatgg ccacttgata ccgcgatacc tcattcgcga taacacgtcc gtcgatattc
     3601 aggctcacat tggtattgat ggtctgactt gccgacgcca tcgcactttg cacttgtgcc
     3661 aaacctgttg taactgcagc cgtatcctgc gccaatgctg cctgatacgc cgccgtctgc
     3721 tgctgaatgg ctgcctgata cgccgccgtc tgagttgaaa acaacggaga caacatctta
     3781 tcggtaggct gcgctgacgg agcagccaaa tactgaggga caccaccccg cgcctgttcc
     3841 cgcttcggcg caggttcaaa tcggataccg ttgtttttca gacgattaat catttcagga
     3901 gttgtcttgg taccacgcaa accctttggc aatgtgccgt cacccaaaga ctcggaatac
     3961 agcaacatac ctaacaaacc cgcccgttta actaacgctg gattattcat gagcccgcta
     4021 ccggcagatt ttccccatga cagcaactta ccgcctgccg ctgtggcacc tgctgccacg
     4081 ccgcctgcgg aagccttacc ccatcccaac gctttactac ctacatcttt cagaaagcct
     4141 gcacctttac cgccaccggc ggttaataac attgcggcag acgccgctgt tgccgccgtc
     4201 gtcaatgctt gcagggctag agtggcatta gggtattccg ctgccagctt tttaaacgag
     4261 gtttccatat ccaccaatgt tcccgtcaga ctttcccgca acattgccag cgattcctgt
     4321 tgctcttgtt gcgccgctgc cgacaacatt cgcgcctcgt tgttcttagc aatttttccg
     4381 ttatccaacg ttacgccagc caacgaagcc atatattcac ggatttgcgt catatccgtt
     4441 gcagccagca aaccttgttt tgcctgcaaa tcaggcagca gttgcgccaa aagcgcgccc
     4501 ttaagcatat ttgcctgctc cgccgccgtc ttatcgcctg cagccgcgcg tttcttataa
     4561 tcttggtatt gcttatcctt tactagcatg gcatcggcaa gacgggacaa cacctgcact
     4621 gcgttttcgc cgttttgctt gccttgcaca accgagccta tccaatcgac acctttcttc
     4681 gggtcattcg gatttgccat cttcttcaga cgacctatcg tgtcaggcga cagagtttta
     4741 ctcaaaagat tttgcacatt agtcgccgct tcggcaggac tgcccgattt attcgccgca
     4801 gattgtaaga gtgagagcag gtagtccaaa ccgccgacac cattcatccc tgcctgttgc
     4861 gcggcagaga gcaggctcgg cagctcccga accatatccc gcacctcgaa agtgccgtct
     4921 aaacccgatt gcaagacgtg ctcaagcccg agttgcaggt ctttaccgct catgccgcca
     4981 tctttcaggg ttttaatcag ttttgccgta tcctcgccac tgccttctga ggcaagtgca
     5041 aaagcatatg ccgcctgcgc ttcattcttg gtttgggcaa aattcagacc ggtggtcatc
     5101 atgccgctga ttaaatccaa agccttatcg tgggtcccgc catttttctc gacaagttca
     5161 agtgccaaat ccttgatctg ttgcgcacct tcagttgcaa tccacgctgc cgatttactg
     5221 ttatcctcaa taaatgcctg tctggacacg cggttgatgt tctcatcaag ctgctttctg
     5281 ttgtccatag caggcttaag caccgtatac gctgccgcgc caccagccac cgcagctgca
     5341 ccgaatcttc cgatttttcc catcttgtcc gcaaatcccg tgccctgttt cagttccgcg
     5401 ttcagctcgc gaattcggtt acgcgtagcg accgccgccc gtgccaaatc attttgtgat
     5461 gccttgccgc tgcgcgccaa acggttaaac gcagcctgtg tcagcatgat ttcgcgttga
     5521 atctgttttt ctgagcggat gccggtcaac gcataagtat ggctttgccg ttcaagttgc
     5581 gcccgcgatt tcgcggtacg atctaattgt cgttgagtat cagccagtag ccgtctcgct
     5641 ccgtcatctc gggcggttaa caccaacgac agtttcatgt tcccgtttgc cataaaaagc
     5701 agcctttaaa cgtattaact caaggtcaat tatcgtttaa aggctgttta agggaggcgg
     5761 tatgggcttc aaccccgttt tcgtgcttta tttgtttgtt gcgttataat actagaatga
     5821 taaccatttt attcaaaggg atattttatg aatatgaaaa aattgatttc cgcaatttgt
     5881 gtttcaattg ttttatcagc ctgcaaccaa caatcaaaaa cggcacaagc cgaagaacct
     5941 gtccaaagta tccaggctgc tgattgtacc gccccaatgg acatcacagt tgaacaatat
     6001 ctcatcaatt tggagcaagc atttaaaact cagaacgtct caacaaaaat ccataataaa
     6061 aatattgtca agaccgattg tggttatgac cttactttgg taatggattt tggggcgatt
     6121 gcgctcaaac tggacgagca gcaaaaaatt agagctatct cagtaggcta cattttaaaa
     6181 accgacggag agaaaggaca aaatctagtc aataatgcca taaatggatt acacagtatt
     6241 caggcagttc tgtctttaac taccacagac aaattgggcg aatcggaagc aggaaaacaa
     6301 ctttttacag ctttaaccga agtcgtcaaa gaatccaatc agacaggagc aacagcgcaa
     6361 aaagacgttc cggcagatgg tattttatat agcgttgttt ttgaaaaaga aacaaacacc
     6421 attgcaataa tcggcagaaa acaaccctaa ccgtcaaata aaaaaaaggt cgtctgaaac
     6481 tcagacgacc tttttatttt ccctccggct ttctcagccg tttcgacaca atcactccct
     6541 cttccttcgg cgtttttatg cccaggctcg acaggatgtc ttcaaaccat gccgacagct
     6601 cggcgtggca catatcgcgc actgcgtccg cccctatccc cgccttgcca tcaggatgac
     6661 cgcctgacgg tagcttttgt gtgccgtcag gtagctgcgt tttactgctc cgccgccgac
     6721 ggcgcatcgg ggctcgccgt agcggcggac ggttttaggc gcaaatcctc ctgagccgcc
     6781 atcacggtat ggtagtcttc ggtagagagg ttttccatca gccactgcgc cgtcaggatg
     6841 tcggatggga taccttgggc ttcaatctgc tgcgaccagt aggtcagcgt ctctaggacg
     6901 tcgcgttgca gcagttcggc ttttgtggcg ttttcgggca gctcgggcaa gtcatcaatc
     6961 agggcgaacg ccgccagttc gccgccgacg gtcagtggct tgaggcgtaa ttcagatacg
     7021 actttgccat tgtaggtcag cccgtgggtc agtttgacgg taatcacgca ttactccttg
     7081 attttgtgca aagcaatcat ctcaatgtcg atgcgtgcct cgttatcgac ttcatattgc
     7141 tcgccggtat cgacggtaaa gcagtcgagg taggaagtgc ggcgatcttc gtcgttgatg
     7201 gggtaaatcg tgattttcgc cttggtgatg ttgtcccagt cgatttccgt accgtcgatc
     7261 ggaatggcgg cggtaacgct taatttgtgt tcggttacgc cgtcacaata accgttgact
     7321 cggccgttgc ggttcatcgt tttgaccggc ttgcggcctg tagtggtttg cggcttgatg
     7381 ctcacaatct cgatatcgcg gccgttcatc tccataatca ccgcgtcggc ataggtagcg
     7441 tcgctcatgc tttatccttt tttaaggttt cagacggcct ttaaacctgt tttaaacgcc
     7501 gtctgaaaag ggttacaaaa tcaaatcaat gcgcccggca aagacgtgca ggccgttgac
     7561 cacatcggcg gggataatgg cattaacacg gttggggtcg ttttgcgcac gcgccaccac
     7621 cagcttgcct ttgttggcct cggcgttttc gatgatttcg gcttggtcga gcttaatcag
     7681 cacgtccaaa atctcgctct taaccttggg cagcaggcgg tcgctcaatt tgtcgcgcgg
     7741 aaaacgcagg gcaatgcgct ctttaacgct gcggcgcaca taatccagcg tgcggatggt
     7801 ggtaatgtcg agtagtgccg ggtcgtcggt gttgttggcc gacttggtat aggtggatac
     7861 ggcacgcata atctgcacgc ggttgttgac cactgtgagc ggggtcaagc cgttgtacag
     7921 cgcattgttg cattctgcaa acagcggcca ttgcgcatca ggtgtaacgg ccagcccttt
     7981 gatttccagc gtgttcagcg gcttggcagg gtcttcttca aaggccaaca ccgccgcata
     8041 acctgcggcg atgatgccgt ttggctctac cgcacctttg taccaagcac aggtcatgcg
     8101 gccgtcgttg atttcgccgg tagcggtggt ggccgtgctc aaggccgcac tcatacccaa
     8161 tacgccgata cagccgcgct gctcgatggc gttggatacg ttggtaatat ggttgctcaa
     8221 ggctttggcg ttggcgtcat cgctaaacgg gctgcaaata atgtgataat gcttgcccgc
     8281 cactttggac aaggccgtgg caatgtccgc atttttggca ccgccggtaa aggcattggc
     8341 ttgataagtt aggccggtat tgccggtgct cacggttaag ccgctctcgt tgccgatctc
     8401 gcctttgtgt ttggccgtca gcgtaacggc tgcgccgctg ccggatgcgg taacggttac
     8461 atcggcggca gtgatggcgg ttttcagacg gtctgccact gtggcggcgg tctcgccggt
     8521 gttaacggcc gtgcttacct gcttgccgcc aatcgtgatt tccaccacgc ccggcgcggt
     8581 ggccgtgccg gaaagggtaa cggttgcggt tgcctgcacg cctgcgctgt ggtcggcaat
     8641 accgataacg gtcaaatcca aataagggtt gttggcaaat gcttggcgca ccatcaaatg
     8701 cgccagcgag ccttgtccga acaaatcggc cgcctcggca tcgctaaata gttgcaccgg
     8761 ctctaaggcg ggctgtatgc ccgcggtcag catgggtgca accatcaata ccttttgcgg
     8821 attttgcggc aaaccttgta cggcattgcg ggtgttaaat tcaatatact gcccgggcac
     8881 gcggatgctg cccggaatcg tgtcaaaatc aatatggggc atcattactc tcccttagta
     8941 ttgcgggttt tggtatttgg ggcggcatcc tcaaccacca ccaaatcgcc gtcatcaatc
     9001 atgcggcggt aatacaggct gttgccgtcc acctccaccg gctcttggcc gatatattcg
     9061 tgcgggttgt gttcggtcgg cacacgcagg ccatctgccg ccgttacttt gattttgctc
     9121 attgcttatc cttaagggtt aggtttacag gtatttcatc ggcggattgc gggtcgtaaa
     9181 ttttgccgtc caacccctcg aaatcaggcc acggctcgct caatgtaccc tgatacttgg
     9241 taaagatatg gttggggtcg tcgggattgt cggtgcgttc ggggtagcgg tcattttcca
     9301 acccgcaggt gttaaagcgg atggcatact caaccgcata tatgcttact gcggcgtttt
     9361 gcaccagcac atgattggca atcgcgcgca ccgctttggg caccaagccg cggctatcgg
     9421 caaaaccgag ccgctggccg tcaagcaggc ggcgaacagc gcggattaaa tcgttgctgc
     9481 cgatttcgcg gctgtcgatg ccgccttgcc gctgcgcctg ctcgttgcgc agattgcggg
     9541 ccgccaccat caccacaaat tcggcggtat cctgataacg tccgcatacg ccgccggtgc
     9601 tggcaggctc aactttgctg ccgccatacg ttacccaaac cgcaggcagc gtatggattt
     9661 gccccgccaa atcgtcggcc tcgccgttgt agcttttaac cgtgcgcacc atgcgaccca
     9721 agccccgttt cagacggtct gttatcgctt gctcaatatc agtgatcacg cccgaacacc
     9781 ttatttttac cgtttacaaa cacaataccg tcttcgcccg cggccacttc ttcgccgcta
     9841 tcatccacgc ccagctgcac ttcgcctttg gccatagcac gcagcaggtc gagcacgtcg
     9901 attttgtagc ggttgcggat ttcgtcggta atcaacacgc cctgagccgc cgtcagacgg
     9961 tagcgggcaa tgtcgcagca aaggcgcacc aagatgggcg gcagatcctc aaaaggtcgt
    10021 ctgaaccgcc ccagatacgc gtcgatttcg gcagtggcgt ccaccagcgc ggtttgtgcg
    10081 acctcgcggt caatcagccc ctcgttgttg cggtcggtga gctgcaagac ttccagctca
    10141 ccgaaacgcg caaccatatc ctcaaccgtc gcgtatgcca ttactcgacc gccttgcgtt
    10201 gcagcatagg ctcggcgcag attgccttcc acaccgcttc gccgacttcg gcgcgcttca
    10261 cttcgcgcca gccgccgtca aacagcaggc cgccgcgcca aaattctttg ccgtctgcgc
    10321 cggtactgac gagcatcaca tcgcggctgt ccgccaaagc gtcggcggca cgttgcgtat
    10381 gctgcacttt gagttcggca agttcggcgg acagtgcctt tttgtcgtct tcggcttttt
    10441 ccaaggctgt ggtcagcatt tcgacatcgt ttcgggcggc ggcaagctct gcctgcacgg
    10501 cgtccaattc ggctttgatg tcttcaaacg acggggcggc ggtttcggcg gtttctggtt
    10561 tgttgttggt ttttgccatg atgactcctt gtttcagac
//
LOCUS       DAWIVB010000055        10453 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWIVB010000055 DAWIVB010000000
VERSION     DAWIVB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.055"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            205..1638
                     /gene="lpdA"
                     /locus_tag="WIX60_001001"
     CDS             205..1638
                     /gene="lpdA"
                     /locus_tag="WIX60_001001"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ5305284.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGSEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGGCRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1733..2020
                     /locus_tag="WIX60_001002"
     CDS             1733..2020
                     /locus_tag="WIX60_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305285.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2119..3285
                     /gene="sucC"
                     /locus_tag="WIX60_001003"
     CDS             2119..3285
                     /gene="sucC"
                     /locus_tag="WIX60_001003"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ5305286.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVDNDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            3296..4186
                     /gene="sucD"
                     /locus_tag="WIX60_001004"
     CDS             3296..4186
                     /gene="sucD"
                     /locus_tag="WIX60_001004"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ5305287.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIIEAVDSGVGLIVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            4215..4439
                     /locus_tag="WIX60_001005"
     CDS             4215..4439
                     /locus_tag="WIX60_001005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305288.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFWGSQRPLQNSPK
                     SPKFPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(4746..6341)
                     /locus_tag="WIX60_001006"
     CDS             complement(4746..6341)
                     /locus_tag="WIX60_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ5305289.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(6375..9224)
                     /gene="uvrA"
                     /locus_tag="WIX60_001007"
     CDS             complement(6375..9224)
                     /gene="uvrA"
                     /locus_tag="WIX60_001007"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ5305290.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPTVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPFD
                     DIHGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIAKGSPEQVAKVKGSYTGKYLKVVLR"
     gene            9379..10176
                     /locus_tag="WIX60_001008"
     CDS             9379..10176
                     /locus_tag="WIX60_001008"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ5305291.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QTESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
BASE COUNT         2664 a         2428 c         2643 g         2718 t
ORIGIN      
        1 gatgtagcgt aatgtagtat cgtgctacaa taggctcaac gaacgattga ggccgtctga
       61 aacatttgat tcgaatgaat cggcagatat ggacttttag acggcttttt cttaaaacca
      121 tcaaaacgca gtcattcaaa ataaaaaaga aacgaaaagt atcgttttta ttttgagata
      181 ctgctaaaag caaaggatga cacgatgtct caatatgatg tagtagtgat tggtgcaggc
      241 ccgggcggat acgttgccgc catccgtgcc gcgcaactgg gtttcaaaac cgcttgtgtc
      301 gacgcaggcg ttaacaaagc aggcaatgcc cctgcattgg gcggtacttg cttgaacgta
      361 ggctgtatcc cttctaaagc cctgttgcaa tccagcgaac atttccacgc tgcgcaacac
      421 gattttgccg aacacggtat cactgtcggc gacgtaaaat tcgacgcggc caaaatgatt
      481 gagcgcaaag atgccatcgt gaccaaactg accggcggcg tgaaattcct gttccaaaaa
      541 aacaaagtaa ccagcctgtt cggtactgct tcctttgccg gtaaaaatgg cgatgcttac
      601 caaatcgaag tcgataacaa aggcgagaaa accgttatcg aagccaaaca cgtcatcgta
      661 gcgaccggtt ccgtaccgcg tccgctgcca caagtcgcta tcgacaacgt taacgtattg
      721 gacaacgaag gcgcattgaa cctgaccgaa gtacctgcca aactcggcgt gatcggttcc
      781 ggcgtgattg gtttggaaat gggttccgta tggaaccgcg tgggttcaga agttaccatt
      841 cttgaagccg caccgacttt cttggctgcc gccgaccaac aaatcgccaa agaagccttc
      901 aaatacttca ccaaagagca aggtctgagc atcgaattgg gcgtgaaaat cggcgacatc
      961 aaatctgaag gcaaaggtgt ttccgttgct tacgaaactg ctgccggcga agccaaaacc
     1021 gaagtattcg acaaactgat cgttgccatc ggccgtattc caaacaccaa aggcctgaac
     1081 gcggaagccg taggcttgga aaaagacgag cgcggcttta tcaaagtaga tggcggatgc
     1141 cgtaccaacc tgcctaacgt atgggcaatc ggcgacgtgg ttcgcggccc gatgttggca
     1201 cacaaagcca gcgacgaagg cgttgccgtt gccgagcgca ttgctggtca aaaaccgcat
     1261 atcgacttca acaacgtacc gttcgtgatt tacaccgacc ctgaaatcgc ttgggtaggt
     1321 aaaaccgaag agcagctcaa agccgaaggc gtggagtaca aaaaaggtac ttcaggtttt
     1381 ggtgcgaatg gtcgcgcatt ggcaatgggc aaagccaaag gtacggttaa agtgttggca
     1441 gacgccaaaa ccgaccgcat cttgggcgta cacatgattg gtccggttgt cagcgaattg
     1501 gttaccgaag gcgtgactgc gctcgaattc ttcgccagca gcgaagacat cgcccgcatt
     1561 atccatgccc acccaacctt gtccgaagtg gttcacgaag ctgcattggc ggccgacaaa
     1621 cgcgctttgc acggttgata gacattaagg cctccgacaa attgaatgtt ccgagagctt
     1681 cgttttctga tttataattc cgtcagacaa acaaacagca tttacattca ttatgaacaa
     1741 agaaatagtc ggtattttct ttataccggc gggcatcatc agcatgtgta tggccgcatt
     1801 gtggcagatg tatgtgatga tgaccgaaac ttatacgctc aaccgtttca aagataaaga
     1861 attggtttgg cgcgtggcat tgttgtttat cagtttcagc cttgccgttt atctgctctg
     1921 tccgaattcg cgtaaaaaag gcatcgtctt ttttattctc gggggaggcg gtgcagccat
     1981 gtatctgctg gcgcggatgt ggttgccttt cagcaagtga aacgacgatt ttccgaccgc
     2041 cgaaaggtag tctgaaacgc acgggcttgc catttggagg caaactcggg gcattccact
     2101 aatctaaagg agaaatccat gaatttacac gagtatcagg ctaaagaact gctggctagc
     2161 tacggtttgc ccgtacaagg cggtattttg gcacacaacg gcgaagaagc cgctgcagct
     2221 tacgacaaat tgggcggcaa attcgctgtt gtcaaagcac aagtacacgc cggcggccgc
     2281 ggtaaagcgg gcggcgtaaa agtcgttaaa agccgcgaag aagctaaaga agtggctgaa
     2341 agcctgattg gcaccaactt ggtaacttac caaaccgatg ccaacggtca acctgtcaac
     2401 agcgttttgg tttgcgaaga tatgtatccg gttcaaaccg agctgtactt gggcgcagtg
     2461 gttgaccgtt ctacccgccg cgttacattc atggcttcta ccgaaggcgg cgtggaaatc
     2521 gaaaaagttg ctgccgaaac tccagaaaaa atcttcaaag taaccgttga tccgctggtc
     2581 ggcctgcaac cttgccaagc tcgcgaagtt gccttccaac tgggcttgaa agacaaacaa
     2641 atcaacgagt tcgtcaaact gatgaccggc gcgtacaaag cgtttgtcga caacgacttc
     2701 gccctgtttg aagtaaaccc attggcagtt cgcgaaaacg gcgcactggc ttgcgtagac
     2761 ggcaaaatcg gcatcgacag caacgcgctc taccgcctgc caaaaatcgc tgaattgcgc
     2821 gacaaatctc aagaaaacga acgcgagctg aaagcttccg aattcgatct gaactatgtt
     2881 gccttggaag gcaacatcgg ctgtatggtt aacggtgccg gtttggcgat ggccactatg
     2941 gacattatca aactgaaagg cggccaacct gccaacttct tggacgttgg tggcggcgca
     3001 accaaagacc gcgtggttga agcgttcaaa ctgattctgg aagacaaatc cgttaaaggc
     3061 gtattgatca acatcttcgg cggtatcgta cgttgcgaca tgattgcgga agccatcgtg
     3121 gcagccgtta aagaaatcaa cgtcaacgtt cctgtcgttg ttcgtttgga aggtaacaac
     3181 gccgaactcg gcgcgaaaat cctgaacgaa tcaggtctga aactgacttc tgcagatggc
     3241 ctgaatgacg cagccgaaaa aattgttgca gccgtaaacg cctaaggaga aaagaatgag
     3301 cgtattgatt aataaagaca ccaaagtatt ggttcaaggt ttcaccggta aaaacggtac
     3361 tttccactcc gaacaagctc tggcttacgg cactaaagtt gtcggcggcg ttaccccggg
     3421 caaaggcggt caaacccacc tgaacctgcc cgtgttcaac accatgaaag aagcggttaa
     3481 agaaaccggc gcggacgcat ctgtaattta cgttcccgct ccgtttgttt tggactctat
     3541 cattgaagcg gttgattcag gcgtaggctt gatcgttgtg attaccgaag gcgtgcctac
     3601 tttggacatg ctcaaagcca aacgctactt ggaaaccaac ggcaacggta cccgcttggt
     3661 cggccctaac tgcccgggcg tgattactcc gggcgagtgc aaaatcggca ttatgccggg
     3721 tcacatccac actccgggcc gcatcggtat tatttcccgt tccggtacat tgacttacga
     3781 agctgtggca caaaccacca aactgggctt gggtcaatca acctgtatcg gtatcggcgg
     3841 cgatccgatt ccgggtatga accaaatcga cgcactgaaa cttttccaag aagacccgga
     3901 taccgacgcc atcatcatga tcggtgaaat tggcggtact gcggaagaag aagcagccga
     3961 atacatccaa tccaacgtaa gcaaacctgt tgtcggctat atcgccggtg ttaccgcacc
     4021 taaaggcaaa cgcatgggtc acgccggtgc gattatctcc ggcggcaaag gtactgcgga
     4081 agaaaaattc gccgctttcg aaaaagccgg tatcgcttac acccgcagcc ctgccgagtt
     4141 gggcactacc atgctggaag tgttgaaagc aaaaggtttg gcataatcag gtttgacaac
     4201 tgattgaaca tcaaatgccg tctgaaaccg gaaatcgggt ttcagacggc attttgtttg
     4261 tcatttcaaa aagaggcagc ctcaacatac ccacattatt tttgcccttt tggggcagtc
     4321 agagaccttt gcaaaattcc ccaaaatccc ctaaattccc taaattccca ccaagacatt
     4381 taggggattt tggggaattt tgcaaaggtc tcgggctaag tgtgcctgtt tgcgcctaaa
     4441 aggcggcccg gatgcctgat tatcgggtat cctgggagga ttaagggggt attggggtaa
     4501 aattagtgga tatttgaaac gaaaacagcc gaaaacctgt gtttgggttt cggctgtcgg
     4561 gagggaaagg aattttgcaa agctctcgta ttggctttga agttccgtgt aattcacagg
     4621 tagggcgtgt ggcacagcca cgcacgcggt cggttgggta tgcaggctac ggctttctct
     4681 gttgaaactg caacacgtca gctaccaacc gtgtgcgtag cgcacacggt tggttgggat
     4741 acaagttact gtttgttctt aaatggagta ccaacatcaa gggcttttga taatcctgaa
     4801 aatattaaat attcagtttc agtttttatt ttaggagaaa tatttgcata atttctatct
     4861 ttaaagcacc aatggatata tggtttcgtt tcatcttctg ggttatctaa ataagcaata
     4921 acattcaagt caaataaaaa ttgcaaaaac tcattagcag tactcataaa tttaggtatt
     4981 tccactgatg ttgtttgtaa gtgctttttc aaacgttcaa atgcttttaa aaaatcacta
     5041 tatttaaatc tatctttccc gtttaaaaat tcaaaaaatt tcaggaaatt ttgataatca
     5101 ctttgactat aataaaacaa aagatgatct ttgatttcac caagtaaata tatcgagtat
     5161 tctctttgaa aagaagtatt atcaaaatct tctgctacga cataatcttc cttacttttc
     5221 ttattttttt gtagcaaagt aagcatctga agaatatcgc gaggtcgata atacgatttt
     5281 cttaggaagc taataaatga agttaaattt ttatactcat catgtaaatt aggagcattc
     5341 catggaaaat aataatccca tgagttgcct ttttctaaac tatcttgttt ttcttgctgg
     5401 gttctcaaaa gatgatcaaa aacgccaaaa atctttgaac ttctataaga tttataatcc
     5461 gtcctccagt ctaaaaatac tgaattatct tgaagtttgg tattttgatt ttgtaaacct
     5521 aatgaatcaa agatatcagg tctaatcaat aacacaactc tcatccttcc cttactatct
     5581 ttaatggaag ggaagatatc attatttaac atccatatgg cgttagcaag accttttaca
     5641 cactcatgat attcatcaaa tggaatctgt gatggtctaa tatctatccc atcaataaac
     5701 aaaatatgat tatcttttag ctttaactga gataaagcat ctttaaattt tctttcaata
     5761 aaacctaaat ttgcttggaa tttactttct gtaaaagtta tttgttggga ttcctcttca
     5821 cctagtttaa caaattttcc aaaaatcatt tccgcagctt cttttgaatt ttctattaaa
     5881 gttattgctt gtacaatttc cggatcaaaa gcgccataat aatattcatt tatagcctca
     5941 tctaaggctt taaatttatt aaatattgaa gataatattc cgttttcttt acatttgatt
     6001 tgatttgata tcaacagata taaaatgact ttccaaatac ttgtaaaatc tgaaacagtt
     6061 aagtgtcttg ctttctttag ctgaataaat tttgaataat cggtttcacg aacaaactta
     6121 gtagtggcat gtatgttttt atagaagtta ttagttaaat aaacagcata tgctgtcttt
     6181 ccagttccct tttctccgat taaaaacgaa atatttggtt cacataattc atccaaatat
     6241 tctcctttta caaatattcg gttaaataaa tctttatttt ctcttcttct gtagtttgca
     6301 gcatccacaa atccaaattc taatgttttt aacggtttca tcttaataat ctcctattta
     6361 attttgaatt aaacttacct caaaaccacc ttcaaatact tcccagtata actcccctta
     6421 actttcgcca cctgttcagg actaccttta gcaataatcc tccccccgcc atctccgcct
     6481 tccggcccca agtccacaat ccaatccgct gttttaatca catccagatt atgctcgata
     6541 atcactatcg agttgccttt gcctttcaga cggcctatga cttccagcag cagggcgatg
     6601 tcggcgaagt gcaggccggt ggtgggttcg tcgaggatgt agagcgttct gccggtgtcg
     6661 cgtttggaga gttccaaggc gagtttcacg cgttgggctt cgccgccgga gagggtggtg
     6721 gcggactgtc cgaggcggat atagcctagg cctacgtcca tcagggtttg cagtttgcgc
     6781 gatacggtgg ggacggcgtc gaaaaattcg cgggcttctt cgacggtcat gtcgaggact
     6841 tggctgatgt ttttgccttt gtattggatt tcgagcgttt cgcggttgta gcgtttgccg
     6901 tggcagactt cgcaggggac gtacacgtcg ggcaggaagt gcatttcgac tttaatcacg
     6961 ccgtcgcctt ggcaggcttc gcagcggccg cctttgacat tgaaggagaa tctgccgacg
     7021 ttgtagccgc gttcgcgaga gagggggacg ccggcgaaga gttcgcggat aggggtgaac
     7081 aggccggtgt aggtggcggg gttggagcga ggagtacggc cgatggggga ctgatcgacg
     7141 ttgatgactt tgtcgaggtg ttcgaggccg tggatgtcgt cgaatggggc gggttcttct
     7201 tgggcgcggt tgagttcgcg ggcggtaatt ttggcgaggg tgtcgttaat cagggtggat
     7261 ttgccgctgc cggacacgcc ggtgatgcag gtaatcaaac cgagcggcag ctcaagggta
     7321 acgtttttga gattgttgcc gcgtgcgcct ttgaggacga gcatccggtc gggattgacg
     7381 ggcgtgcgtt cagacggcac ggcaatggat tttttgccgc tgaggtattg tccggtaacg
     7441 gagttttcgc attgggcgac gttttcgggc gtgtcggcaa tcagtacgtt gcctccgtgt
     7501 tcgcctgcgc cggggcccat atcgaccacg aaatcggctt cgcggatggc gtcttcgtcg
     7561 tgttcgacca caatcacgct gttgcccaaa tcgcgcaggc gtttgagggt ggccagcagg
     7621 cggtcattgt cacgctggtg caggccgatg gagggttcgt ccagtacata catcacgccg
     7681 gtcaggccgc tgccgatttg gctggcgagg cggatgcgct gggcttcgcc gccggagagg
     7741 gtttcggcgg agcggcttaa attcaggtaa tccagcccga cgttaatcag gaagccgagg
     7801 cgttcggtga tttctttgag gattttttcg gcgatttgtt ttttgttgcc gtctaaatcc
     7861 agtgtttcaa agaattggtg ggttttggtg agcggccagg cggaaacttc gtgcaacggc
     7921 tcaccgctga cgtaaacgta gcgggcttct ttgcgcaaac gtgcgccgcc gcagcttggg
     7981 caggcgcggt ggttttggta ttcgcgcagt ttttcgcgca cggtttcgct gtcggtttcg
     8041 cggtagcggc gttcgagatt ggggatgatg ccttcaaagg cgtggctgcg gttgaaggtg
     8101 gtgccgcgtt cggacaggta agtgaaatca atgacttctt tgcctgagcc gtgcagcaca
     8161 acttttttca ctttttcagg tagtgtttcc caagcagcct gcacatcgaa accgtaatgc
     8221 cgcgccaatg attgaatcat ttggaaatag aattggttgc gcttgtccca accgtcaatc
     8281 gcacctgttg ccagcgacaa ttcgggatgg gcgaccactt tttcggggtc gaagaaattg
     8341 gtgttgccca agccgtcgca agtcgggcag gaacccatcg ggttgttgaa cgaaaaaagg
     8401 cgaggctcta attcgggcag gctgtacgaa cacacggggc aggcgaaacg tgcggaaaac
     8461 caatgttctt cgccgctgtc catctccatc gccagcgcac gctcgttgcc gtggcgcagc
     8521 gcggtttcaa aactttccgc cagccgctgc ttgatgtccg ccttcacttt cacgcggtcg
     8581 atgaccacgt cgatattgtg cttgatgttt ttttccagct tcggcacttc gtccaactga
     8641 tagacctcgc cgtccacgcg cacccgcgca aaaccctgcg cctgcaagtc ggcaaagaaa
     8701 tcgacaaact cgcccttacg ctcgcgcacg gtgggggcaa gaatcatcac acgcgtgtct
     8761 tccggcagtt tcaatacggc atcgaccatc tgcgatacgg tttggctcga cagcggcagc
     8821 ttgtgttcgg gacaatacgg ggtaccgaca cgggcgtata aaagacgcag atagtcgtgg
     8881 atttcagtta ccgtaccgac ggtggagcgt gggttgtggc tggtggattt ttgctcgatg
     8941 gaaattgcag gcgacagacc ttcaattaaa tcgacatcgg gtttgtccat catctgcaaa
     9001 aactgccgcg cataggcgga aaggctctcg acataacgcc gttgcccttc ggcatacagc
     9061 gtgtcaaacg ccagcgacga cttgccgctg cccgacaatc ctgttaccac cacgagtttg
     9121 tggcgcggaa tgtctaaatc gatgtttttc aaattatgcg tgcgcgcgcc gcggatgcgg
     9181 atggtgtcgt tgtcgtgcga atgttgggga tgatggttgc acataatgga tgccgcctga
     9241 aaaataaagg aaaaccggta ttgtagcact ttctcggatg ccgtctgaag ccgcgttcag
     9301 acggcatttg ccagcggagt acggcagatt ccgctataat gtcggcaatt ttaacccgct
     9361 tgaacaaaag gatgacaaat gaaccgtctt tacccccacc cgattatcgc ccgtgagggc
     9421 tggccgatta ttggcggcgg tttggctttg agcctgctgg tgtcgatatg ttgcggctgg
     9481 tggtctttgc cgttttgggt gtttaccgta tttgcattgc agtttttccg cgaccctgcg
     9541 cgtgagattc cgctaaatcc tgaagcggtg ttgagcccgg ttgacggccg tatcgtggtg
     9601 gtcgaacgcg cacgcgatcc gtatcgtgat gtcgatgctt tgaaaatcag tatttttatg
     9661 aacgtgttca acgtgcattc gcaaaaatcg cctgccgatt gtacggtaac gaaagtggtc
     9721 tataacaaag gcaaattcgt gaatgcggat ttggacaaag ccagcacgga aaacgaacgt
     9781 aatgcggtgt tggcgactac ggcttcaggt cgtgaaatta cttttgttca agtggccggt
     9841 ttggtggcgc gccgtatttt gtgctacacc caagcaggtg cgaaactgtc ccgcggcgaa
     9901 cgttatggct ttatccgctt cggttcgcgc gtggatatgt atctgcctgt cgatgcgcag
     9961 gcgcaagtgg cgattggcga taaagtaacc ggcgtcagca ctgtattggc gcgtttgccg
    10021 ctgactgcgc cgcaaactga atctgagcct gaatctgagc ctgctttaca aactgctccg
    10081 gttgaaacag cggcaaaccc atctgccgaa caacggcaaa tcgaggcagc ggcggctaag
    10141 attcaggcgg ctgtgcaaga tgtgttgaaa gattaatttt gcggactgaa atagaaaata
    10201 tcagtaccat cattcacacg aatgaggaag tttggttttt tgaatttttg ctaatgttca
    10261 caccgtcatt cccacgaaag tgggaatcta gaaacttaac gttacgacga tttatcggaa
    10321 acgactgaaa ccggacggac tggattcccg cctgcgcggg aatgacgact tattagttac
    10381 ctaacactta aaaaacagaa acctttccgc gtcattccca cgaaagtggg aatccgggaa
    10441 cttaacgtta cag
//
LOCUS       DAWIVB010000056        10408 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWIVB010000056 DAWIVB010000000
VERSION     DAWIVB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.056"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..462
                     /locus_tag="WIX60_001009"
     CDS             <1..462
                     /locus_tag="WIX60_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ5305292.1"
                     /translation="AQDMRNIVASILRNDYGLTVRTDGEGKGNMPLRDAVKLIRGSDV
                     AIEFHTNAAANKTATGIEALSTPKNKRWCQVLGKAVAKKTGWKLRGEDGFKPDNAGQH
                     SRLAYAQAGGIVFEPFFISNDTDLALFKTTKWGICRAIADAIAMELGAAKV"
     gene            459..662
                     /locus_tag="WIX60_001010"
     CDS             459..662
                     /locus_tag="WIX60_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305293.1"
                     /translation="MKKSLIALCVAHCAKLKNDFGVPPLPEIKITPSPVRVGSLKQHP
                     SLRLGKSGVAAAKRAARKRKNRR"
     gene            665..829
                     /locus_tag="WIX60_001011"
     CDS             665..829
                     /locus_tag="WIX60_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305294.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYREML
                     KRHLQTKSVE"
     gene            832..1068
                     /locus_tag="WIX60_001012"
     CDS             832..1068
                     /locus_tag="WIX60_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305295.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            1040..1459
                     /locus_tag="WIX60_001013"
     CDS             1040..1459
                     /locus_tag="WIX60_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305296.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVID
                     KMERDKAQALLLSAQNYARELELARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNASGGCIDGFGSHGLQLYNRALGYGN"
     gene            1479..1661
                     /locus_tag="WIX60_001014"
     CDS             1479..1661
                     /locus_tag="WIX60_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305297.1"
                     /translation="MPTPPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQN
                     QAWRDWAGNHSRKVGN"
     gene            complement(1706..1876)
                     /locus_tag="WIX60_001015"
     CDS             complement(1706..1876)
                     /locus_tag="WIX60_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ5305298.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            1889..2107
                     /locus_tag="WIX60_001016"
     CDS             1889..2107
                     /locus_tag="WIX60_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305299.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            2104..2442
                     /locus_tag="WIX60_001017"
     CDS             2104..2442
                     /locus_tag="WIX60_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305300.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            2454..2720
                     /locus_tag="WIX60_001018"
     CDS             2454..2720
                     /locus_tag="WIX60_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305301.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            2717..2914
                     /locus_tag="WIX60_001019"
     CDS             2717..2914
                     /locus_tag="WIX60_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305302.1"
                     /translation="MSDPILDALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            2917..3423
                     /locus_tag="WIX60_001020"
     CDS             2917..3423
                     /locus_tag="WIX60_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ5305303.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(3420..4010)
                     /locus_tag="WIX60_001021"
     CDS             complement(3420..4010)
                     /locus_tag="WIX60_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305304.1"
                     /translation="MNWLNRGIQEKILRVLSDNYPNCLKATKVYNSLFPSSSLPITSN
                     IPVDKQGLGLALTLGCEIQSQHLATLQSDEFQYFLKNIYYLEESGLIEITSIDSLHKN
                     FDCKINHKGIDFLTDDGGLSAILGVVTVKLHSDTIQALIAAKIDQAEISDSEKSWLKK
                     ELGKIKDTALSTLTENAINAIPAATLITLLKKFVGL"
     gene            4023..5645
                     /gene="terL"
                     /locus_tag="WIX60_001022"
     CDS             4023..5645
                     /gene="terL"
                     /locus_tag="WIX60_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ5305305.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTK
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRVTG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            5645..7213
                     /locus_tag="WIX60_001023"
     CDS             5645..7213
                     /locus_tag="WIX60_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ5305306.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     NGTTATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            7200..8495
                     /locus_tag="WIX60_001024"
     CDS             7200..8495
                     /locus_tag="WIX60_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ5305307.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            8605..9020
                     /locus_tag="WIX60_001025"
                     /pseudo
     CDS             8605..9020
                     /locus_tag="WIX60_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
     gene            9251..10312
                     /locus_tag="WIX60_001026"
     CDS             9251..10312
                     /locus_tag="WIX60_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305308.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
BASE COUNT         2734 a         2444 c         2891 g         2339 t
ORIGIN      
        1 gcgcaggata tgcgcaacat tgtggcttca atcctgcgta acgattacgg cctgaccgta
       61 cgcaccgacg gcgaaggcaa aggcaatatg cccttgcgcg atgcggtcaa gctgattcgc
      121 ggctcggatg tggcgattga gttccacacc aatgcggcgg cgaacaaaac ggcgacaggc
      181 atcgaagcct tgtccacgcc gaaaaataaa cgctggtgtc aggtgctggg caaagccgtt
      241 gccaagaaaa ccggctggaa actgcgcggc gaagacggct ttaagccgga taacgcaggg
      301 caacattcgc gcctggctta tgcgcaggca ggcggcattg tgtttgagcc ttttttcatc
      361 agcaacgaca ctgatttggc cttgtttaag acgaccaaat ggggcatctg ccgcgcgatt
      421 gcggacgcga ttgcgatgga attgggagcg gcgaaggtat gaaaaagtct ttgattgctt
      481 tatgtgttgc ccattgtgca aagttgaaaa acgattttgg cgtaccaccg ttacctgaaa
      541 tcaaaatcac gccaagccct gttcgggtag gctctttgaa acaacatccg agcctgcgct
      601 tgggtaaatc aggcgtggcg gctgctaaac gtgcggcgcg caaacgcaag aatcgtcgtt
      661 aatcatggga caggttgcgt tttacgaaaa gatgattggg ctgtggtcgg ccaaaagccg
      721 tgaggcaagc gaacaggcgg acttggctgc gtttgaattt gcggagggcg aactggccaa
      781 ttatcgggaa atgctgaaac ggcacctgca aaccaaaagt gtggaatagc aatgcgtatt
      841 ttggatattt ttaaaaaccc ggcgacaggc aatgtgtcgc actcgaaact gtgggcaaac
      901 gttgcctgcg cggctgggac gtttaagttt gtgatgttgc ccgatccgtc ggcggaaatt
      961 tgggcggtgt atttgggcat tgtcggcggc tatgcggtgg cgcgttcatt tgtcagcgtg
     1021 aagcgtcagg aggtcgagaa tgaatctcgt gaaactgctg gcgaataact ggcaaccgat
     1081 tgccattatc gcgcttgtcg gcacgggctt ggctgtgtcg caccatcaag gctacaagtc
     1141 ggcatttgcg aagcagcagg cggtcatcga caagatggag cgcgacaagg cgcaagccct
     1201 gctgttgtcg gctcaaaact atgcgcgcga actggaactg gcacgcgcgg aagctaaaaa
     1261 atatgaagtc aaggcgcacg ctgtcggcat ggctttggcg aaaaaacagg cggaagtcag
     1321 ccgtctgaaa acggaaaata aaaaggaaat cgaaaatgtc cttactcaag accgtaaaaa
     1381 tgcaagcggc ggttgcattg acggctttgg ctctcacggc ctgcagctct acaaccgcgc
     1441 cctcggctac ggaaattaag gttgtcgaaa aggcagtcat gccgaccccg cctgctgcat
     1501 tgatggtcgc gccggtacgc ccgaatccgc cgaaagacgg caagacggcc acgctgttgg
     1561 aacacgccgc tgagtttggt ggctatgtgg cggagttgga aaatcaaaat caggcttggc
     1621 gcgactgggc gggcaatcac tcccgcaaag tcggaaactg acaaaaaagc ccgcgtaggg
     1681 cgcgggctga gggtgaaagc ggattttata cctcttttac aggggtagcg gcggtagtgc
     1741 ttttcagcaa atcgactgcg tgctgacagt tttgcttgct ggtgtagcct tcgccctgag
     1801 cgatgatttc atggttggct gctttcaaac gccaacggta ttcgcctttt gcgtctttat
     1861 agatttcaaa atacataagg tttctcctat gaatgagtac acgttttctt accgctttaa
     1921 cggcaagtcc tggtcattga gcatttgggc ggacaaccct gaagaagcca gggcgaaatt
     1981 tcgggctgca cgagaaaatg cgcactatga cggcgaagtt gtagcaaagg tttatacatt
     2041 tgtaaatatt tcgtgggtta agaaattgta caagcggaca aaatatttaa tgggtatcaa
     2101 agaatgacct accgtgaatt agttgaacgt cagttggctg tgcgccatgc cgatttggaa
     2161 ttgggcttaa gccgcgcacg cgagcaagag ccgtttgtca ttcatgtttc cgatctgttg
     2221 gataaggcag gcattgagta cgcggtacgc atggataagg attttcagac gacgtttcac
     2281 cttgaatatc caattacgaa ctatgacacc tttaaacgtg cggtttggca aactttgggg
     2341 gcgtattact gtgtttgtaa tgatggtgat ggactggaga ttgccagcaa tcgccctgac
     2401 ggttacgccg tccgtatcgt attcggcgat gtgccggttt aaaggggttt taaatggact
     2461 ttgaatttgg tttcagaacc ctgtggccga ttgcgacggc ggcattttgg ttttgggtca
     2521 acggcatttc aggccgcctg aaagaggcgg acaagcgtat cgacgacctt aaagaggagt
     2581 tgcacgcggt caagctctct tatcacacca aggcggacgc caaggcagac agcactaata
     2641 ttgcggcggc cttggagcga attgaaaaca agttagaaaa agtaaacgaa aaactggaca
     2701 ggaaagcaga caaatcatga gcgacccgat tttggatgcc ttggcgcgta ttgaaaacaa
     2761 gactgatcaa acgctgaaaa atcagaagga aatgcaggcg gaaattgcgc aaattcgcca
     2821 agacacgaaa cgcacggcca ttacattcgg cgcactgggc ggcggcgtga ttacggtcgg
     2881 ctgggaattg cttaaagcga aaatgggact gtaattatgg ctcacccgca agaaatccgt
     2941 gaaaagttac gccggctcta tgtgagcggc gagcaaactt tggaaacggc ggccttgatg
     3001 tgcgaaatcc cgcagaccac tgcgcgtgcg tggaaacgtg cggataagga aaaaggcgac
     3061 gactgggata agatgcgcgc cgcttacact ttggccggtg gcggtattga ggatttgagc
     3121 cgtgcgatgt tggccggttt tatggtgcag tacaacagca cgatgacgat gctgcaagat
     3181 tcgagtaccg aagatttgcc accatccgac cgcgccaagc tgttggccag cctggccgat
     3241 gcgtttacga aaaccgtatc ggccaatgcg cgtgtgatgc cggaaacgtc aaaactggcg
     3301 acggctttgg aattgattga gttcttgatg gcgtttgtac aagaaaaaca tcccaaacat
     3361 ttgcctgcct ttgtggaggt attggagccg tttggggtgg aagtggagaa gaagtttggt
     3421 tagaggccaa caaatttttt taaaagagta atgagggtgg cggcagggat agcattaatt
     3481 gcattttccg taagcgtact taatgcagtg tccttgattt tccctaattc ttttttcaac
     3541 cagctttttt ctgaatcaga aatctctgct tggtctattt ttgccgcaat taaagcctga
     3601 atagtgtcgc tgtgtagttt gactgtgaca acaccaagaa tggcggatag gccgccatca
     3661 tcggtaagga agtctatgcc cttatgatta attttgcagt caaaattttt atgaagtgaa
     3721 tctatcgaag taatttcaat taaaccagat tcttctaagt aataaatatt ttttaaaaaa
     3781 tattggaatt catctgattg taaagttgct aggtgttgac tttgaatttc acaaccaaga
     3841 gtcaaagcca agcccaatcc ttgtttatca acagggatgt tagaagtaat aggtaaagaa
     3901 ctactaggga aaagagagtt atacaccttg gttgctttta ggcagttcgg gtaattatca
     3961 cttaaaactc gtaagatttt ttcctgaata cctctattta accagttcat aaattattcc
     4021 tcatgaaaac aaaagaattc ctcaaatccc ttgccgaact ggccgccagt ttgcgccaag
     4081 tcatcgaagc ggaagtggac ggcttcgatg cgtcgcccaa ggctattgct gcacgccgtg
     4141 ccaaggtgtt tgacccggta ggcggttacg agtatttcgt gaatacctac ttcccccatt
     4201 atatccgctc gcctgagaaa tccgaactgc atgcgttttt attcagccgt ctgccggaga
     4261 ttatccgctc ccccaaaggg gaaaatgagg cggtgggtgc gccgcgtgga gagggtaagt
     4321 cgacgaaggt tactcagttg tttacgctgt ggtgtattgt gaccggccaa aaacattatg
     4381 ctgttattgt gatggacagt atcgaccagg catatccgat gctggaagcc atcaaggcgg
     4441 aacttgaatt taacccgcgc ttgaaaaccg actttccgga agtatgcggg cagggccgtg
     4501 tatggcaggc cggtacgatt gtgacggcca atgacgttaa agtccaagtg gccggtagcg
     4561 gtaaaaagct gcgcggtttg cgtcacggcc cttaccgtcc tgacttaact gttttggacg
     4621 atattgagaa tgacgagcaa gtccgcaacc ccgaacagcg cgacaagctc aatgcgtggc
     4681 tgactaagac cgtattgcct ctgggcggtg tcggtcagaa atacgatgtg atttatatcg
     4741 gcacgatttt gcattacgac agcgtactta accgcacttt gaataacccg ttttggcacg
     4801 gtattaagtt taaggcgatg aaacgctggc ctgaccgcat ggatttgtgg gacaggtggg
     4861 aggaactttt ccgaaacgac ggcgagacgg tggccgaggc gttttatcag gcaaacaaag
     4921 acgagatgga gcgcggcgcg gtcacttctt gggcggcgcg tggcgtactc gcgctgatga
     4981 aaatccgtgc gcgtgacggc catgcgacgt ttgattcaga atatcaaaac gatccggtca
     5041 gtggcgaaga tgcgccgttt gccaagtcga tgaagttttg gaacgacctg ccgtccgatt
     5101 tggtgtattt cggtgcgctc gacccgtcac tcggaaaggc cggggcgagc cgtgacccgt
     5161 ccgcgattat cattggcggt tatcaacgtg taaccggcaa actgtatgtc gtggaggctc
     5221 agattaaaaa acgtctccct gatttgatta ttgaggacgt tattcgattg caccgtcaat
     5281 atcgttgcaa actgtggttt gttgagactg ttcaatttca ggaatttctg aaagacgagc
     5341 tggtcaagcg cagcgcggcg cgtggaatac ctgtcccagc gcgggcggtc aaaccggtat
     5401 cggacaagct gttgcggatc gagactttac agcctcacat ggcgaacggt ttgattctgt
     5461 tgaatgagag ccaacaaacg ctgatacagc agttccgcca ttttccaaag gctgatcatg
     5521 atgatggtcc tgatgccgtg catatgctct ggtcgggggc ggtggccaat tgtgtgccga
     5581 tagaatggca aagccctacc gataacgatt ttgatgacga gataaaaagt aaatggagcc
     5641 gataatggca aaaaagaaca ataaaactaa aatccaaaag cccgaagctg cattgcagac
     5701 ggacgtggct caaattacgg cgaccggtcg ggttatcgcc gagcatccgt ccaattttat
     5761 tacgccgcaa aagatgcggg ccctcttcga ggacgcagaa agcggcgaca tccgcgccca
     5821 acacgagctt ttcgcggaca ttgaggagcg cgacagcgac atcgcggcaa atatggggac
     5881 gcgcaaacgc gcgctgctga cgctcaactg gcgcgtcgcc ccgccgcgaa atgcgacgcc
     5941 cgaagaagaa aagctgtccg accaagccta cgaaatgatg gacagcctgc ctaccctcga
     6001 agacctgatt atggatttga tggacgcggt agggcacgga ttttctgcgt tggaggtcga
     6061 gtgggtattt tcagacggcc tttacctacc ccgaaacttt atccaccgcc cgcaaagctg
     6121 gttcaaatgg gacaaagaca acgggctgct gctgcgtacc cgcgaaaatc cggaaggcga
     6181 agcgttgtgg ccgctgggct gggtcgttca tacccaaaaa tcgcgcagcg tccagcaggc
     6241 gcgcaacggg cttttccgca cgctttcctg gctgtatatg ttcaaacact acgccgtcca
     6301 cgattttgcc gagtttttgg agctgtacgg catgcccatc cgtatcggca aatacggcgc
     6361 gggcgcaacc aaagaggaaa aaaacaccct gcttcgagcg gtggcggaaa tcggtcacaa
     6421 cgcggcaggc atcatgccag aaggtatgga aatagagctc cacaacgcgg caaacggtac
     6481 gacggcaacc agcaatccgt ttttgcagat ggccgactgg tgcgaaaaat cggcggcgcg
     6541 gctgattttg gggcaaacgc tgaccagcgg tgcggacgga aaatccagca ccaacgcgct
     6601 gggcaatatc cacaacgagg tacgccgcga tttgctggtg tcggacgcaa aacaggtggc
     6661 gcaaaccatc acaagccaaa tcatcggacc gttcctgcaa atcaactatc cccatgccga
     6721 cccaaaccgc gtgccgaaat ttgaatttga cacgcgcgag ccgaaagaca tcgcggtctt
     6781 tgccgacgct atcccgaaac tggtggatgt cggcgtacaa atccccgaaa gctgggtgcg
     6841 cgacaaactg gtcattccag atgtgcagga gggtgaggct gtgttggtgc ggcaggtacc
     6901 ggacaatccg gtaaacagaa ctgcattggc ggctttatcc gcccacaccg taccatctaa
     6961 ggctacgggc aggcatcagg aaatattgga cggcgcgttg gatgacgcgc tggttgagcc
     7021 cgatttcaat tctcagctca acccgatggt gcgtcaggcg gttgccgcac ttaatgcttg
     7081 caacagctac gaggaggcag atgccgcact gaatgcgctt tatccgaatt tggacaacgc
     7141 gaaactgcgt acctatatgc agcaggcctt gtttatcagc gatattttgg gacaagacca
     7201 tgcccgcgcc tgatttggga tttgccttaa gtctgccgcc aaaaaaggca atcgagtggc
     7261 tggaaagtaa aaaggttacg gcggagagct accgcaatct gacagcctcc gaaattgcca
     7321 aagtctatac gattgcccgc atgaccgact tggatatgct caacgacatc aaaacttcga
     7381 tggttgaatc ggcaaaaagt ggacagtcgt ttgacgattg gcgaaaaggt atcttgaatc
     7441 tgctcagcaa caagggctgg ctgcatccga acgggcataa cggtaaggat atcatcgacc
     7501 cagccaccgg cgaggtattc ggttcgccgc ggaggttgga gacgatttac cgtaccaata
     7561 tgcaaactgc ctacaacgcc ggtcaatatc aaggatatat ggcaaatatt gatgcacgac
     7621 cttattggat gtatgacgcg gtaggcgaca gccgcacccg tccggcgcat tcggcaatag
     7681 acgggctggt gtaccgctac gacgacccgt tttgggcaac gttttacccg cccaacggct
     7741 acaactgccg ctgctcggtc atcgcgctgt cggagcggga tgtggaacgc caggggcgga
     7801 ttgttgggca aagcacggcg gacaatctgg tcgagaccca taaaatctac aacaaaaaag
     7861 gcgatactta tctgaccctt gcctataaag caccggatgg cagtctgtac acgaccgatc
     7921 gaggatttga ttacaacgcc ggacgaatga actaccgccc cgatttagac aagtacgacc
     7981 gtgcgttggc gcatcaattt gccaaagcgg aaatgggtgg tgcggatttt aaaaccagct
     8041 ttaaacagct tgaaaaagag ttttatgaag tcaagcaacg tttggatatt gatggcaagc
     8101 ccgataaaga gcagaaaatc aaaatccgaa atgcgctatc aagacagctt aaatttgctg
     8161 cgggtgtatt gagcaaggaa acgcaagaat tggcaggtat gacacgagcg acggtgtggc
     8221 tgtctgatga tacgttggtt aaacaggtag acagccgtga ggggcagaat ttcgatgact
     8281 cctactatgc ttttttgccg gatatgctgc aaaaccctga acatgtcatc cgcgacaatc
     8341 gtgaattgat tttcacagct cgctataaag gctcggcatt gtgggcagtt ttaaaatata
     8401 ttaaggaggt ggatgagatt tatctacagt cgtaccgaat cagtaacgac aaagagattg
     8461 ccaaatttat ggcgaagaag aaagtattga aatagacgtt gggcaaggct cgaaatcact
     8521 tgcacacgct ctcggacgcc ctaacgggca ggctgcggta tcgaggttat caccgctttt
     8581 ccaacgtctg tacggaataa taccatgatt gatgtcaaaa tagacaatat ctttgtcgtc
     8641 ctaaaccaaa tcgagcggct tggcaacggg atcgaaaacc gctacctgct gatgcgccga
     8701 ctgtccgaaa ccatgcacac ggcggtcaag ctcaatttcc gctacgcagg ccgtccgaaa
     8761 tggttgggct aaaataccgc gacggcaagc cgctttcgga ttcgggtcgt ctgaaagaca
     8821 gtttttccac actgtcagac aacgatacag cccttgtcgg tacgaatatc gtctatgccg
     8881 ccatccacaa cttcggcggt atggcggggc gcaaccgcaa agttcggatt ccgcaacggg
     8941 aatttttgac gctgacggac gacgacaaac aggctttgat ggacgatgtg caggattatt
     9001 tttcgggtct gataccgtga atttataaaa ccctcaaaaa cgcgcttttt agcgcgtttt
     9061 tttatgcggg taatacaaac ccctgcccaa gatataaaaa tcaatcctag acgcttctaa
     9121 aaagcccctg aaaacgatta attgtgtatc gcgcggacag gttttaaaaa aatggcggga
     9181 gggtttgaag cacgcctact ctttgttgtt ttttcaaata ggcaaaatga ccgtattgag
     9241 agaggtacac atgtccaaaa atgcacaaaa aaccctactt gccgtgtgca gtttcgaggt
     9301 gcagccaaaa gacgggcgaa tccaactgct gccatatggc gaatttcgcg cagtagacgg
     9361 tcgtccgact gatgtccctg cgtggtatct gaccgaagaa aacggtcatg atgtcgcgtt
     9421 gttggccaac agctcgcgca atcagttggt tgtcgattat gaacaccaga cgctctacaa
     9481 agagaaaaac ggacaacctg cacctgccgc cggttggatg cgttggctgg agttcacgcc
     9541 taaaggcatg tttgccgaag tggagtggac ggacaaggcg gctgcggcaa ttgccgcaaa
     9601 agagtatcgc tacatctctg ctgtgttttc ctatgacaca aagggatatg taagcaaaat
     9661 ttttcacgcc gcgctgacaa atttccccgc gttggacggt atggacgagg tgctggcggc
     9721 agcgtcggcg caaattttaa aaccggaaac ggagcaaaac cctatgaaag agttgttaca
     9781 gcaactgttc gacctgcctg atgcgggcga agaagaactg aaggcggcat tgtccgcgct
     9841 cgtggaagcc aagccgaaag acgtggcatt gtctgccgac gtgttcgcgc agctggcgga
     9901 aaaagacagc cgcatcgcgg cattgacggc gcaaaccgcc aagcctgatt tgactaaata
     9961 cgcgcctatc tcagtggttc aagagctgca aagcaaagtc gccgcgctga ctgccaagca
    10021 ggaagcagac aaaggcaacg aattgattac cgccgcgctg acttcaggca aattgctgcc
    10081 tgctcagaag gagtgggcaa aaggcgtatt gaaacagccg ggcggcttgg catttttgac
    10141 cggctttatt gaaaacgccc agccggtcgc tgcactggca ggctcgcaaa cgggcggcaa
    10201 agcacccgac gaacgcgtcg ccgcactgac tgcggaagag gcagccgcag caaaaatgct
    10261 gggcatgtcc ggcgaagaat ttgtaaaaat caaagaaagc gaaggtaagt aatggacaaa
    10321 tcagcgattt tgaccgcaat cacggcagca ttccgcaaag aatttcaaac ggccttggat
    10381 tcggatttca agtgcaacac taaggtac
//
LOCUS       DAWIVB010000057        10087 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWIVB010000057 DAWIVB010000000
VERSION     DAWIVB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.057"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(109..1782)
                     /gene="recN"
                     /locus_tag="WIX60_001027"
     CDS             complement(109..1782)
                     /gene="recN"
                     /locus_tag="WIX60_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ5305309.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRLLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1966..2568)
                     /locus_tag="WIX60_001028"
     CDS             complement(1966..2568)
                     /locus_tag="WIX60_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ5305310.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLGLN
                     SLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WIX60_001029"
     CDS             complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WIX60_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ5305311.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WIX60_001030"
     CDS             complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WIX60_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ5305312.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4526..5488)
                     /locus_tag="WIX60_001031"
     CDS             complement(4526..5488)
                     /locus_tag="WIX60_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ5305313.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNEDRPD"
     gene            complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WIX60_001032"
     CDS             complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WIX60_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ5305314.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEVRAILTEE"
     gene            complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WIX60_001033"
     CDS             complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WIX60_001033"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ5305315.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7044..7520)
                     /locus_tag="WIX60_001034"
     CDS             complement(7044..7520)
                     /locus_tag="WIX60_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ5305316.1"
                     /translation="MEHLFGKWLPDLPAAISDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVAGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WIX60_001035"
     CDS             complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WIX60_001035"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ5305317.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGIPMQEADKRRLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WIX60_001036"
     CDS             complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WIX60_001036"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ5305318.1"
                     /translation="MPSEHQHISSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLNRIFYADSGSISVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLHDLCDEFDIMLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            9697..9812
                     /locus_tag="WIX60_001037"
                     /pseudo
     CDS             9697..9812
                     /locus_tag="WIX60_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2334 a         2747 c         2568 g         2438 t
ORIGIN      
        1 gcacaatcac ttgttcaagt gattaagaaa gcgtgataat acggatttat ctgagttctg
       61 tctattgttt tagactttta tttttagtta ttttttaact gattgatttt aaaataaact
      121 atttttcgac gcaagttgca gcaattctgc cgcatgttgc cgcgtcgtat cggtaatgac
      181 ttctccgccc aacatacggg caacctcttc gatccgttgg atttcatcca atatactgat
      241 ttcgctgacg gtttgctctc cctcgctgtg cttgcgcacc cgccagtggt tttctccgca
      301 ggatgcgact tggggaaggt gggtaacggc aagcacctga tgttttctgc ccaacgcacg
      361 taatgccttg ccgaccattt cagccactcc ccctccaata ccggtatcga cctcatcaaa
      421 aatcagggtg ggaacttggg tatattggct ggcaacaacc tgtaaggcaa ggctgatacg
      481 cgccaattcg ccgccggagg caactttatt cagcagacgg ggcggattgc ctttgttggc
      541 ggcaacttga aattgaacct gctccaaacc gtgtgccgtc ggcgacgaag gcaacaggac
      601 gatgtcgaaa cgcgcgcctt tcatggcaag gtgttgcata tgctcggtcg tttcgccgct
      661 caaacgctct gccgcctgat ggcgcatggc agaaaggatg tgggcagctt cctgatattc
      721 ggcaaaattg tgggcaacat tatgctcgag cgcgtccaaa tcggcggcag cttgcaggct
      781 ttgcaggcgt tcttcgattt ctgccaactt ggcaggcaac tcttcaggct cgatccggta
      841 tttccgcgcc atccccatca gctcgcccat acgctgctct tgtgcggcaa gttcgttggg
      901 attgatgtcg ctgcgacctg ccacatcgcg catattggca ctgatttcgc ccaattcggc
      961 ttcgatgctt gccaacatat tcaggctctc ggcaaagcgc ggctcgatgt tttgcagatt
     1021 ggccaatagt ttttgacatt gatagatatg gcgttggatg ccgttgtcgc cgtcaatctt
     1081 gcttccgact tcttcggcag cctgcaacag ctcggcagaa tgggcaaggc tgtcgtggct
     1141 ttggctgagg gcttcccact cgccttgttt aatgtccaac tgattcaatt cgttaaactg
     1201 ccattccaga cgctcccgct cgataatgac ggcatcggcg tgttcctgcg cctcttggag
     1261 ggcttttttc gcattggccc aattttgata aagctgcctg acggtttccg cctgctccct
     1321 gctacccgca aatgcgtcca acaattcgcg ctgggcggct tcctgattaa gcgaatgatg
     1381 ggcgttttgc ccgtggatgt cgataagctg gctaccgacg gctttgagtt gcgccaaggt
     1441 agcggcctga ttgttgataa agctgcggct tttgcctttg gcatcgataa tgcggcggat
     1501 actgagttct tcttctccgt cgtttaaaag cccctgttca tacaattctg cttttaaaac
     1561 aggtaaatgg gaaatatcaa acaacgccga caactgcgct tcttttgcgc cgctgcggac
     1621 ttggctgtaa tcggctttat cgcccaacag cagaccaatc gcatccaaag taatggactt
     1681 gcccgcgcca gtttctccgg tcaatacggt aaagccgctt tgaaaatcca gattcagatt
     1741 ttcaacaatg acaaaatcac gcaaagaaag tgttagaagc atcgtgagga ttccttagca
     1801 attctaccga caaaagcaat atcaacttta gatatacgaa ttataactaa gattttttaa
     1861 attaaaaata agtttttcct taatttttct tagtgcttgt ttttatctgc ttatttgttg
     1921 caatgccgtc tgaagcaatg tgcgtttcag acggcatttg gaatttcagt tgggcagggt
     1981 atcggacggt acggtttccg gctcttcttc atccgtaggc ggcatttcta tcaaatcggg
     2041 caaaaccagt tcgcccagtt cggtcagcgg cggcagttct tccaagctgt tcaaacccaa
     2101 atcgctgagg aacgttgccg ttgtcgccca caatgcgggt tttcccaatg tgtcccgatg
     2161 tccgatgact tcaatccacc cccgatcctg caaggtctgc atcacgttct gcgacaccgc
     2221 cacgccgcgt atgccctcga tgtcgccgcg cgttacgggc tgctggtagg cgataatcgc
     2281 cagtgtttcc atcacggcgc gggagtagcg cggcgcacgc tgttcttgca ggctgcccag
     2341 ccgctcgaat gccgtctgaa caatctgaaa acgccagccc tcttgcgtat gcaccagttg
     2401 caacgcccta tcctgccaac gcgttttcaa ctgcgccaac acatcaatca gtttgtcttg
     2461 cgacaacggc ggcacacaca gttcgcgcat agatttttcg gtcagcggtt cggtttgggt
     2521 cagcagtgcg gcttcaatca gcgcgtcggg agaaattttg tcggtcatac gggtatccgt
     2581 gctgaaacgg catgggtttg gatatgccgt ctgaaatcgg ttggagtaga gagaagctgc
     2641 ctgaaaaata tttttcagac ggcattcttt atgcttccga aggcttctgc cctttacgct
     2701 ctgcttttct ggcttcgcgt atggctttga gttcggcttc tttctgacgg gcttttttga
     2761 gccaagtttc ccattggttc ggcgtttcga gggtgattct gccgattttg ccttcacgga
     2821 agtcggtgag gatgttttcg gcggcttttt ggtagttgat ccgtccgccg ctgaggactg
     2881 cgccgcgttt tttggctatc cattcgagcc aaacgttttc gtcccagtgg ctgctggggt
     2941 ctttgtcggc ttggtagcgt tcttgcaaca tagggaggta gtggcggcgg aggtagtcta
     3001 aaagttcgag ggcgacttct tcttcgtcca acgcgttgcg tccgactgcg ccgccggcgg
     3061 caaggttgta gccgccttct tcgacgatga ttttcggcca tagcattccg ggggtgtcgt
     3121 agagccagaa gtcatcggcg aggaagaggc gttgttcggc tttggtgatg ccgggttcgt
     3181 tgccggtttt ggcggatttt ttgcctatca tgccgttgat gagggtggac ttgccaacgt
     3241 tggggatgcc gcagatgagg acgcgcaggg gtttatctat gccttggcgg tggggaatca
     3301 tggcacgaca ggcttgggta attttgccgt gtgcgcctgt ttcggaggaa tcgagggcga
     3361 tggcgcaggt gtcggggcgg ctgttatagt gttcgagcca gattttggtg cgctcggggt
     3421 cggcaagatc ttgtttgttg aggattttaa gtttgggttt acctttggaa agctgggcaa
     3481 gcagggggtt ttcgctggag gcgggcatac gcgcgtccag catttcaatc accatatcaa
     3541 cgctttttgc acgctcggcg atggcttttt tcgccttgtt catatggccg ggaaaccatt
     3601 ggattgccat gtctgttctt tcttttcaat atttgaaatg ccgtctgaaa cggaggacgg
     3661 ggtttcagac ggcataatgg tttgacggaa ttagcggtct gacaggtttt gcgcctgtct
     3721 gtgccgtatc agcaattcat aacgcccttt caaccttcgg gcgagttttt cgacaatata
     3781 gaccgaacgg tgctgtcctc ccgtgcaacc gatggcgacg gtaacgtagc tcctgctttc
     3841 atcctccaaa cgcggtaacc aatgcgtaac aaacctttcg atgtcgtcaa ccatttcctg
     3901 cacaagcggc tgtccgtcca aataatccca aacgggcttg tccataccgg tgtaaggcct
     3961 caactcggga tcgtaatacg ggttgggcag gctgcgcata tcgaacataa aatccgcgtt
     4021 gttcggcaca ccgtatttga acccgaagga ctccaaaatc accagcagcc cggtacgttc
     4081 gaccttcagc cactgccgga ctgcatggcg gagctgttgg gcattcatct tggaagtgtc
     4141 gatacaatag gcgatttctt taagcgggaa cagccattcc cgttctttct ttaagctttc
     4201 caacaaggtc atatcctgat tgctcagagg atgtcctcgc ctggtttcgg aaaaccggcg
     4261 gaccaacacg ctttcttccg cctcgacaaa caaaacttca accctgtgcc ccagtccgcg
     4321 cagagaggca atctgttccc gcgcctgtcc gatgtcaatg ccggaacgca catcgacgct
     4381 gaccgccaat tcggtttcgt ccgcacgttc gatatgatac gacaccagcg cgggcaacat
     4441 ttccaaaggc aaattgtcca cgcagaaata acccgaatct tccatttggc gcagtgcgac
     4501 ggacttgccc gaaccggaca ggccgctaat caggacgatc ttcattgtgt tgttcgtttt
     4561 ctttaagttg cgtctgatgg cgttccaaaa attcgcgcgt actgtcctta ccgcgcaact
     4621 gcaaaatgta attgcgtacc gccgcctcaa ccaaaacggc gaggttgcgt ccgacggcga
     4681 cgggcagcgt aaccgaacgg acgttgacgt tgaggatgga ttcggtttcg gtgcggatgc
     4741 tcaaccggtc aagctgcttc atatactcgt cgtccgcctc gactaaattg ataatgagtt
     4801 gcaggatttt tttggggcgg atggaagttt cgccgaaaat atggcggata ttgagtatcc
     4861 ccaagccgcg cacttccaaa aaatcgcgca gcataggcga acaacgccct tccagcgttt
     4921 ccgggccgat gcggaacagc tcgaccgcat cgtcggcaat caggctgtgg ccgcgcgaaa
     4981 tcagttccaa tgccaattcg ctcttaccca ggccggaatg cccggtaatc agcacgccga
     5041 tttcaaacac atcgagaaat acgccgtgtt tgacggacga tgccgccaag gtgcgttgca
     5101 ggtaaatccg caacacgtcc atcagatagg ggctttcgag tttggaagtc agcagtggaa
     5161 tatcgttttt atgacaatag tcgcgcagtc ccggggaaac cggcaagccg tttgccacaa
     5221 taaccaaaga catagaaata tcgaacaggt cgccaaactg ataacccgtt tcccccgatt
     5281 cgaggcggtt cagatattcc gactctgcca aaccgaccac ttggatttgg ttgggatgaa
     5341 tgaaattcag gtgtccgact agggcgagga cgggcttgtc cgcctctacg ccgatacggt
     5401 tgtccgcacc cgaattgccg gcggcccaag cgagttgcag tttgtattgg ttgtcatcaa
     5461 acaggcggcg gacggagata ctgggcatat tattcttcag tcaggatggc acggacttct
     5521 tccgcagagg aaaccgtcat cagcgattct ctgatgcttt tttgggaaaa cttgccggcc
     5581 agtttggata agacttccaa atgctcgccg gttgcgtttt ccggaaccag caagataaaa
     5641 atcagggaaa ccggcttgcc gtccggtgcg tcaaatccga cgggttcgcg cgtgcggatg
     5701 aacgcgcccg tcgcctgctt cacgccggca tgacggccgt gcgggatggc aacgccctgc
     5761 cccaaaccgg tcgaaccgag tttttcacgg gcaaaaagac attcgaaaac atcagcatgg
     5821 gacaatgagg attcgcgttc caaaagcagg cctgcttcct caaacagcct ttttttactg
     5881 cctacctcca tatccaaaac aatatgggac aaaggcaaaa tttcgccgat aaggctcata
     5941 agcttctctt ttcagacatc gcaaaacaga aagattgtac cgactgccgg ggcaaatctc
     6001 aatcccgcat acggtacggg ctgacataac acagcgtttt aaaaacatat tttaacgctt
     6061 ttcggcacag atagaaatgc cgtccaaagc agtttacggc tcttcagacg gcattgccct
     6121 gccttatttc gcgaaaaacg tatgggcaaa aatggcggta aaccacaccc aaccaattct
     6181 gttgtttgcc aaaaacgttt caaaacagat ttgccggacg cggcttttga tggcggcata
     6241 ttggcggtat tgcagcagca ggacgatggg gattgccgtc caatatgccc atgccgcacc
     6301 gataaccgca cccaatactg ccatcagcag ggtaaagcct ccgtgacaca gcataacggc
     6361 ggcgatgtcg taacgcccga acgtgacggc ggaggttttg atgccgattt tcaaatcgtc
     6421 ttctttgtcc gccattgcat aaaccgtgtc atacgccaga gtccataaca cattggcggc
     6481 aaagagtatc cacgcttgag gcggcacgtt tccggcaacg gcggcaaacg ccatcgggat
     6541 accgaaggaa aaggcaagcc cgagatagag ttggggaatc ggaaaaaaac gtttggtaaa
     6601 cgggtaagtc agcgcaagaa acagcgcggg caggctcatc agccaagtca gatgattcag
     6661 cggaatcagg cacaatgcgg caagcaggca caaaaatgcc gtcagcagca gcgcttcttt
     6721 tttcttgacc ctgccctgtg cgaacggacg gttttttgta cgctcgacag caccgtcaaa
     6781 atcgcggtcg gcaaagtcgt tgatgacgca gccggcactg cgcattaaaa acgtgccgat
     6841 tgtaaacgcc gccaataccg ccaaatcggg aatgccgtct gaagccagcc acaatgccca
     6901 gtaggtcggc cacagtaaaa gcagcgtccc aatgggcttg tccgcccgca tcaggcgcag
     6961 gtacacatcc aaacggtcgg acaggcgtaa aaataaaggg gatttaggat tcatattgcc
     7021 gcgcagcttg aaaaaacggt attttatccg ataaaacgtt tcagttcggg cagaaaatac
     7081 tcggtcagca gcatttcctc gccgtgacgg gaaaaccgag aacgccgcgc ggcaaagtac
     7141 cgtccgcatc cttcgccggc aacggcaaac tcaaacgccg aacgcgcccc ttccaaatcg
     7201 gcttgaaaca gacgctcgcc caaaggacgc gtgccgcagt ccaaaatgtt ttgccaaaac
     7261 gccgaaccga tacggcattc gctccttgcc tcaacaacag ggatacggtc cagcttcaac
     7321 aaaacttcgc gcaccagcct ccctccgcat tccgtctcca attcgcccag tttcagcagt
     7381 tccaccgaaa atgtatgcgg caaggcgcgc aatgcggcgg tcagcgaccg ggtgtgcagc
     7441 agccgcacca tcggcaggct gatgccgtct gaaatggcgg cgggcaagtc gggcagccat
     7501 ttcccaaata ggtgttccat atttttccca atctttatac cgcgtctgtt tttgccaact
     7561 ccatccattc cgcttcggaa aaatcggctt tcagacggca tttcaagtag ctcaggctgt
     7621 cttgggcgat gtgtgcgtcg cggctgtcgt ggatgtggtt gaacacgagg ctgtgcaagc
     7681 gaatgccgta ttgtttgagc gcggcgaaac tgagtaaagt gtggttgata ctgccgagcc
     7741 gtccgctggt aacgaggatg acgggatagc cttgctgacg gatataatca atggttaaca
     7801 ggttttccgt cagcggaacc atcaatccgc ccgcgccttc gaccaaaacg acttcgtact
     7861 gcgccgccaa ttcttgtgtg gcggtgcgga ttttgtccaa gtccaaagcc ctgccatcca
     7921 gtcgggcggc gaggtgaggc gaagcgggat agctgaagat ttcgggcata gtcagccgcc
     7981 gtttgtcggc ttcctgcatc ggtatgccca taattttgcg gtggacggcg atgtcgtcgg
     8041 caatgttttg gcaaccggtt tgcacgggct tttgcgtaat cacgcttttg ccctgctgca
     8101 acaattgttt tgccaacacg ccggtggcga cggttttgcc gatgtccgtg tctatgccgc
     8161 tgacgaagta aacgcctttc atttgctgtg ttccttcaag atttgcacgg ttttgtcggc
     8221 aagtttggtc aaaacgccgt ctgaaatgat atagggcggc atcagataca ccagcctgcc
     8281 gaacgggcgc acccaaatgc cctgcgccac gcagtccgct tgaaaacgcg ccatatccac
     8341 gcctttttcc agctcgatca ccccgatggc acctaaaacg cgcacgtctt tcacgccgcg
     8401 aatgtcccac gcggctttca gacggccttt taagatgctt tcaatgcggc ggatatttgc
     8461 ctgccagtct tgagacaaaa gcagtttgac cgaagcgcag gcaacggcac acgccagcgg
     8521 gtttgccata aacgtcgggc cgtgcataaa cacgcccgct tcgccgcgcg aaatcgtttc
     8581 ggtaactttt tgcgaagtga ttgctgccgc cagcgtcata tagccgccgc tcaaaccctt
     8641 gccaatacac ataatatccg gcacgacctc cgcgtgttcg caggcaaaca tcttgcccgt
     8701 gcgcccgaat ccagtggcga tttcgtcaaa aatcagcatg atatcaaatt cgtcgcacaa
     8761 atcgtgcaat ccgcgaagat actgcggatg ataaaaatac atgccgcccg cgccctgcac
     8821 gaccggctct aaaataaagg cggcaatatc cgcatgatgc acttcaaata aggcgcggac
     8881 aggctgcaaa tccgccccgt cccattcatc gtcgaaacgg cttttcggat tatcgacaaa
     8941 ataacgctgc ggcaacgcgc tgccgaaaat atggtgcatc cccgtttccg gatcgcagac
     9001 ggacatcgcg ttccaagtat cgccgtgata cccgcggcgc accgtcgcga tattctgctt
     9061 cgccgtcaaa ccccgcgcct gctggtattg cactgccatc ttcagcgcaa cttccaccga
     9121 aatcgaaccc gaatccgcat aaaaaatacg gttcagcccc tgcggcaaaa tcccgaccaa
     9181 caacttgccc agctccaccg ctggctcgtg cgtcaaacca ccgaacatca cgtgcgccat
     9241 ttgtttcatc tgcgtctcaa ccgcctgatt caaaacagga tgattgtagc cgtgtatcgc
     9301 acaccaccag gaggacatcc cgtcaatcag ccgcgtgccg tccgccaatt cgataaacac
     9361 cccttctgca cgtttgacag gataaacggg cagcggatcg gtcatggaag tatagggatg
     9421 aagcagatgg gtacggtcga aatcaagcaa tgatgatatg tgttgatgtt cagacggcat
     9481 aagtttctct cttttcttct tactgtattc aaacgcaaaa cgcgtattct actccgacag
     9541 accgtttccc acacctctcc atccgtttcg ggcgcaaaac cgcgaaacaa atcgtccgca
     9601 gtataagcgc acaccgtttc gcattcccca agcccgattg gaatcagacg gcccaacgcc
     9661 caataccgtt tcacgcagcc tccgcccaac gcttcaatca gtcatagata tagtggatta
     9721 acaaaaatca ggacaaggca acgaagccgc agacagtaca aatagtacgg caaggcgagg
     9781 taacgccgta ctggtttaaa tttaatccac tataaaacgg caatccatac gatacagatc
     9841 atagcaacag ccatcgcaac agcgttagca aaatcagggg actccgacat aggcgcatag
     9901 cacctaccga tgcacggctc ctcattcggc tctatgaata ccatacccat cacaaaatcc
     9961 accgccaaaa ccaggcacgg cttcttatac ttatgataga tttccaccat cctgtcccat
    10021 atataccaaa cattcatacc gtatatcccg caggcaacaa attccgattg aaggttacag
    10081 ccctatt
//
LOCUS       DAWIVB010000058         9863 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWIVB010000058 DAWIVB010000000
VERSION     DAWIVB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.058"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..168)
                     /locus_tag="WIX60_001038"
     CDS             complement(<1..168)
                     /locus_tag="WIX60_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305319.1"
                     /translation="MPSETFPFQTAWFYSNLNRADRSQKHTFAIPAQALPLYAPNPKP
                     PHSRRHYSGLNF"
     gene            complement(193..2250)
                     /gene="ppk1"
                     /locus_tag="WIX60_001039"
     CDS             complement(193..2250)
                     /gene="ppk1"
                     /locus_tag="WIX60_001039"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ5305320.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDQNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKREHKRCPQRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTDTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPAVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNILFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYYFHNNGTDDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIHEGLTMALDDNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2419..2862)
                     /locus_tag="WIX60_001040"
     CDS             complement(2419..2862)
                     /locus_tag="WIX60_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ5305321.1"
                     /translation="MPFRLFLGILYGLSMVAPVYAQGQPDTVGDFIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(2869..3384)
                     /locus_tag="WIX60_001041"
     CDS             complement(2869..3384)
                     /locus_tag="WIX60_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ5305322.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDESMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            3654..6284
                     /locus_tag="WIX60_001042"
     CDS             3654..6284
                     /locus_tag="WIX60_001042"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ5305323.1"
                     /translation="MQEQYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            6418..7221
                     /locus_tag="WIX60_001043"
     CDS             6418..7221
                     /locus_tag="WIX60_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ5305324.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDNS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKSINCAQLPRYFDFKS"
     gene            7341..7472
                     /locus_tag="WIX60_001044"
     CDS             7341..7472
                     /locus_tag="WIX60_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ5305325.1"
                     /translation="MRDFDKPAEEAAEYAAEGAVKFIEGKPAKKIIAVPGRLENIVV"
     gene            7495..8424
                     /locus_tag="WIX60_001045"
     CDS             7495..8424
                     /locus_tag="WIX60_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ5305326.1"
                     /translation="MLRCRPKSVLDSDGIFLKFNFLRSKPKYEISFLPSFKRILCLSA
                     VISVLGACAVVADVYGHDSATMNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFR
                     RMLPYADAANNTSHKFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAI
                     MGHEMTHALHEHGKNKVGQQILTNTAAQIGTQIILDKKPDTNPELVGLGMDILGTYGL
                     TLPYSRSLEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTHPTNN
                     ARIENLKRLLPTVMPVYEQSVRNKGRVNKKRRR"
     gene            complement(8807..9118)
                     /locus_tag="WIX60_001046"
     CDS             complement(8807..9118)
                     /locus_tag="WIX60_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ5305327.1"
                     /translation="MKYKNEALAAIHEMMEGAYNIGAIDKKTMRDFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(9115..9483)
                     /locus_tag="WIX60_001047"
     CDS             complement(9115..9483)
                     /locus_tag="WIX60_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ5305328.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADKAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
BASE COUNT         2320 a         2544 c         2671 g         2328 t
ORIGIN      
        1 aaaatttaat ccactataat gccgtctgga atgcggcggc ttggggttgg gagcgtatag
       61 gggaagtgct tgtgcaggaa tggcgaaggt gtgtttttga ctccggtccg ccctgtttag
      121 attgctgtaa aaccatgccg tctgaaacgg aaaggtttca gacggcatgg tttttgattg
      181 gggcggtgcg ggtcagcctc cgagcagtgt ccacaaatcg ttttgcaggt cggcttcgga
      241 ttcgccctcg gcaggtgcgg cgcggatata gccgccgtcg ggctgcatca gccacgcgtg
      301 ggtgttgtcg tccagtgcca tggtcagtcc ttcatgtata acgcgctttt tgagttcggg
      361 cgcggtaatc ggcgtggcgg tttcgatgcg gcggaagaag ttgcgcccca tccaatccgc
      421 gctggagata aaggtatcgt ccgtgccgtt gttatggaag taatacacgc gcgcgtgttc
      481 gagctgcctg ccgacgatgg agcggacgcg gatgttttcg gacaagcctt ttacacccgg
      541 gcgcaaggtg cacataccgc gcacaatcaa atcgatttgt acgcctgccg cgcttgcccg
      601 atacagggct tcgatgacgg tcggttcgat gagcgaattc atcttggcgg taatccgcgc
      661 cggtttgccg gcttttgcgt gttcggtttc gcgtgcgatg cggtcgataa ccattttgtg
      721 cagggtaaac ggactttggt agagtttgtt cagccgcccg ggtttgccca aacctgtgat
      781 ttccataaac aatatgttca catcggcggt gatttgttcg tcggcggtaa tgaggccgaa
      841 gtcggtgtag atgcgcgatg tgccttggtg gtagttgccc gtgccgagat gggcgtaacg
      901 tttgagcacg ccgtcttcgc ggcggatgac cagtgccatt ttggcgtgga ctttgtagcc
      961 gaacacgccg tacacgacgt gcgcgcccgc ctcttcgagc tgcttcgccc agttgacgtt
     1021 gttggcttcg tcaaaacgcg ccatcagttc gacgacgacg gttacttgtt tgccggcgag
     1081 tgccgccttc atcagggcgc ggacgagttc ggaacgcgtg ccggtgcggt aaatcgtcat
     1141 tttgacggca agcacggcgg ggtcggcggc ggcttcgcgc atcatttcga caacggggtc
     1201 gaacgattgg taggggtggt gcagcaggat gggcgattgg cgcaccaaat cgaatatgga
     1261 cgcggttttg cccaaggctt tcagtctgcc cggcgtgtgt gtgggaaatt tcaaatcggg
     1321 gcggttgact aggtcgggga cggcgttgag gcgcacgagg ttgaccgggc ctttgacctg
     1381 atagagttcg gcggcggtca gtttgaattg cgcgagcaga aagtcgcgga tgtaggcggg
     1441 acaggtgtcg gcgacttcga gccgcacgcc gtcgccgtat tcgcggtcgt gcaactcgtt
     1501 ttgaatggcg gcgcggaggt tttgcaggtc ttcttcgtca acggtcaagt cgctgtcgcg
     1561 cgtcaggcgg aactggtggc agcctttgac gttcatgccc gggaagagtt ttccgacgtg
     1621 ggcgtgcagg atggaggaga ggaagacgaa gccgtgtccg ccgccacaca gttcggacgg
     1681 caggggaaca acgcgcggca ggatgcgtgg tgcctgcaca atcgccatcc ccgaaggcct
     1741 gccgaacgcg tctgtgccgt cgagttcgac ggcgaagttg agcgatttgt tcagcgggcg
     1801 cgggaagggg tgggaagggt cgagtccgat gggggtcagg atcggcagca attcgcggtc
     1861 gaaatagtct tcaatccatt ttttctgtgt gtctgtccaa tttcggcggc ggtaaaaatg
     1921 gatgccttct tgtgccagct cgggctgaag gacgttgttg aacaggtcgt actggtgccg
     1981 tatcagggag cgcgccgctt cggtaacgtc ggcgatggtt tcagacggca ttttgccgtt
     2041 gtccagcctg cgctgcgggc agcgtttgtg ttcgcgcttc agccacgcca tacggacttc
     2101 gaaaaactcg tcgaggttgg atgaaacgat gcacaggaag cgcaggcgtt ccaaaagggg
     2161 gacgttttgg tcttccgcct gcgccaacac gcggcggttg aatgccagca agctcagttc
     2221 gcggcagagg atgcggtttt gttcgggcat aaggttctcc caaagatggt tgttgttcgg
     2281 tcggagcggg caggcaatgc cgtctgaagg cttggcggca tctgcgccgg tttcagacgg
     2341 catgacgtgg cggacagtct gtttcaggca tcggggcaat gtgaaaacag ataccggcgc
     2401 gtgcggtatc cgtctgtttc aaatcacttc caacacaaaa taatgacgca gtttttcgta
     2461 caccgcatcg ctgacgttga tgcagccgtt ggtcataatc ctgtcggaca cggacgggga
     2521 ggcgatgcgt tcgttccgcc tttccgacgg tatctgattc caaacgcggt gcagggcgaa
     2581 aagaaaatca ccctcctgct tgaaaccgat gacttcgccg ccgtatccgg gcttgtcggt
     2641 actgttcagc gtcagcccga acgtcccctt gggcgtggca acaccgatga ggacggggtg
     2701 gcactgacgg tcgtcagcga aacagagttc cgctttggat gtatcgacga tgactttttt
     2761 cttttggata aagtcgccga ccgtatccgg ctgcccctgt gcatatacgg gggcaaccat
     2821 tgacaggccg tataatatcc ctaaaaacag gcggaacggc atgtcggatt attggcggat
     2881 acgtttcggt tttgccggca cgatttcgcg gatgatgact tgcggctcgg ctttgggtgc
     2941 gggcggcgga caggcggcat ctttcgggaa gacggggttc cagtagaagc tgcgggcaaa
     3001 tttgtcttta tcgaaaatca ctttgtattg gcaggtagta acatcttcta cgccggaagt
     3061 gttttcaggg tcgataccta cgcccggggt atggaagtgg aacaggtaat cccattcgcg
     3121 cacgccgtac atactttcgt cgtaatgcgg gcggcccagg attttgtaga tgtcgtcttt
     3181 ggtcaggccg gggcgcatct gatccagttc gtcataagtc gggaatgtgc cgcgcttgtt
     3241 gtcgagcgtt acggaatagg gtttcgggaa aaccggattg tcggtcgtgc cgtcggcttt
     3301 gacgttgctt ttggttgcgc aggcagacag aacgcccgcc gccaatactg ccaagcccgg
     3361 tttgacgatt tgtttgattt tcatatgcag aatcctcttt tacccgatgc ccgtctgcct
     3421 tttgttcgga agagccgcat caggagagtt ttaaacattt gattttggtt tgtaatatta
     3481 gcataaaaaa cgtgcgtatc aataaacgcg gtgtatttgt acggcatacg gaataatgcg
     3541 cgtgcgaatt tacgcatcct gccggcaatc tgccgattcg ccgcttccgg caacctgtta
     3601 taattgatgc cgactttatt tttccaacgt ttttaagcga aaacagaatg actatgcaag
     3661 aacaataccg gcccgccgcc attgagcctg cggcgcagaa aaaatgggac gacgcccgta
     3721 ttttcaacgt ctccgaagac gcttccaaac ccaaatatta ttgcctctcc atgttcccct
     3781 atcccagcgg caagctgcac atggggcatg tgcgcaacta caccatcggc gatgtattga
     3841 gccgcttcaa acttttaaac ggcttcaacg tcatgcagcc tatgggttgg gacgcgttcg
     3901 gcatgccggc ggaaaacgcg gcgatgaaaa acaacgtcgc ccctgccgct tggacgtacg
     3961 ataacatcga atacatgaaa acccagctca aaagcctagg ttttgcgatt gactgggcgc
     4021 gcgaaaccgc aacctgcaaa cccgaatact accgctggga acaatggctg tttaccaagc
     4081 tgtttgaaaa aggcatcgtt tatcgcaaaa acggcacggt aaactgggac cctgtcgacc
     4141 aaaccgtcct tgccaacgag caagtcatcg acggacgcgg ctggcgttcc ggcgcattga
     4201 tcgaaaaacg cgaaatcccg atgtattact tcaaaatcac ggattacgcc gaagagctgc
     4261 tcaacgactt ggacaagctg gaacactggc cggaacaagt caaaaccatg cagcgcaact
     4321 ggatcggcaa atcgcgcggt atgaccgtgc gcttcgccgt ttcagacgac agcaaacaag
     4381 gcttggaagg cgattacgcg aaattcctgc aagtttatac cacccgcccc gacacgctga
     4441 tgggtgcgac ttatgttgcc gttgccgccg agcatccgct ggcagccgcc gcagccgccg
     4501 acaaacccga attgcaggca tttattgccg aatgcaaagc cggttcggtt gccgaagccg
     4561 atatggcgac gatggagaaa aaaggcgtgc cgaccggccg ctacgtcgtc aacccgctca
     4621 acggcgacaa gctggaagtg tggattgcca actatgtatt gtggggctac ggcgacggcg
     4681 cggtgatggc ggttccggcg cacgacgaac gcgattttga gttcgccacc aaatacaatc
     4741 tgccgaaaaa acaagtcatt gccgtcggcg acaacgcatt tgacgaaaac caatggcaag
     4801 aatggtacgg cgacaaagaa aacggcgtat tggtcaacag cggcgacttg gacggcttgg
     4861 attttcagac ggcattcgac gccgtcgccg ccaagctgca aagccaaggc gcgggcgaac
     4921 cgaaaaccca ataccgcctg cgcgactggg gcatttcgcg ccaacgctac tggggctgcc
     4981 cgattcccat cgtccattgc gaacaatgcg gcgacgtacc cgtccctgcc gaccaattgc
     5041 cggtcgtttt gcctgaaaac gtcgtacccg acggcatggg ttcgccgctg gcaaaaatgc
     5101 ccgagtttta cgaaactgcc tgcccatgct gcggcggcgc ggcgaaacgc gaaaccgaca
     5161 ccatggacac cttcatggaa tcgagctggt atttcttccg ctacatgtcg ccaaaatttt
     5221 cagacggcat ggtcgatcct gccgccgcga aatactgggg cgcggtcgac caatacatcg
     5281 gcggcatcga acacgcgatt ttgcacctct tgtacgcgcg tttcttcacc aaactgatgc
     5341 gcgacgaagg tctagtcaat gttgacgaac cgtttgaacg cctgctgacg caaggcatgg
     5401 tcgtttgcga aacctactac cgcgaaaacg acaaaggcgg caaagattgg atcaaccccg
     5461 ccgatgtcga gctgacgttc gacgacaaag gccgccccat ttccgccgtc ctcaaagccg
     5521 acgggctgcc cgtcgtcatc agcggcacgg aaaaaatgtc caagtccaaa aacaacggcg
     5581 tcgatccgca agaactgatt aacgcctacg gcgcggacac cgcccgcctg tttatgatgt
     5641 tcgccgcacc gcccgaacag tccctcgaat ggagcgacag cggcgtcgaa ggcgcacacc
     5701 gcttcctgcg ccgcctgtgg cgtaccgttt acgaatactt gaagcaaggc gaagcggtca
     5761 aagcgttcgc aggcagccaa gacggtttgt ctaaagaact caaagacctg cgccacaaac
     5821 tgcacgcgac cactgccaaa gtcagcgacg actacggccg ccgccagcag ttcaacaccg
     5881 ccatcgccgc cgtgatggaa ttgctcaacc aatacgacaa aaccgacacc ggcggcgaac
     5941 aaggacgcgc cgtcgcccaa gaagtattgg aaaccgccgt acgcctgttg tggcccatcg
     6001 tgccgcacat ctgcgaaacc ctgtggagcg aattgaacgg cgcgaaactg tgggaagcag
     6061 gctggccgac agtcgacgaa gccgccttgg tcaaatccga aatcgaagtg atggttcaag
     6121 tcaacggcaa actgcgcggc aaaatcactg ttgccgccga cgcatccaaa gccgacctcg
     6181 aagccgccgc actcgccacc gaaggcgcgg tgaaattcat ggaaggcaaa ccggcgaaga
     6241 aaatcatcgt cgtaccgggc agactggtga acatcgtcgt ctaaccgctt ttaaggttta
     6301 gccacacgga taaaggccgt ctgaaacttg gaaacagggt ttcagacggc ctttttaagg
     6361 tggattgtgt tgtctgcatg acgaaccccg aataactgaa atctcaaaat ccaaaacatg
     6421 aatttatttt tcgataccga attgggaaag caacaaaata aagcaaccca taaaatccgt
     6481 gtaatgagcg aggcttggtt ggaaaaaaac ggctactgcc cctgttgcgg aagcaagccg
     6541 atgcagagat ttgccaataa caaacctgtt gcagacctct tttgcccaaa ttgccacgag
     6601 caatatgaat taaagagtaa aaatcaaaaa accataggca acagcgtgcc tgacggtgca
     6661 tatcacacca tgttggagcg catccggtca gataccaacc ccaacttttt ctttcttgca
     6721 tataaaaaag cggattactc catacggcaa ttggtgcttg tacccaaaca tttcatcacg
     6781 ccggatatga ttattcccag aaataaaggc attaaaaacc ggccgaacca cattatgtgt
     6841 tctatcaatc tcgccccttt gcctgaaagc ggcaaaatat tcttaataga caattcccgc
     6901 attatcgaac ccgaaaccgt tctgaaaaaa tggcaatcca atttgttttt acgcaaccaa
     6961 aatgcggagc gcaaaggctg gcttctggct gttatgaaat gtatcgacca actccccgaa
     7021 gaattcacat tgtcgcaaat gtatgaattt gaaaacaaac tatccatcca atttccccaa
     7081 aacaaccata tcagagacaa aatccgccaa cagttgcaaa ttctgcgcga ccaaaatacg
     7141 atcgaattca ttggtcgcgg actttacaaa aaatcaataa attgcgccca actcccaagg
     7201 tattttgatt tcaaatcatg atactgaatt cacaagggat atttattctt ctgccatttt
     7261 gaaaacagtt gaagttcagc gcaatattgc cgcaaaaggc agcagcagcc gtaaaaaagt
     7321 tggagaatta ttggcaattt atgcgtgatt tcgacaaacc ggctgaagaa gctgccgaat
     7381 acgccgctga aggcgcggtg aaattcatag aaggcaagcc tgccaagaaa atcatcgccg
     7441 taccgggcag actggagaac atcgtggtgt aaatcttttt aaaaacatta aacaatgtta
     7501 agatgccgtc cgaaatccgt tttggattca gacggcattt ttttgaaatt taatttttta
     7561 aggagtaaac ctaaatatga aatttccttc cttccttcct ttaaacggat actctgcctg
     7621 tcggcagtaa tctcggtatt gggggcttgt gcggtcgttg ctgatgttta cggtcatgat
     7681 tccgccacaa tgaacgctgc ggctgccaaa gattatatga aaacggttga gttaaacaag
     7741 tctgccggca atgtcgatac cacatccaga acagcccgca gggtgcaggc agtatttcga
     7801 cgtatgctgc cttatgccga tgcggcaaat aataccagcc ataagtttga ctggaaaatg
     7861 acggttttca aaaacgatga gctgaacgcg tgggcaatgc ccggtggaaa aatggcgttt
     7921 tatacgggga tagtcgacaa actcaagctg accgatgacg aaattgccgc cattatgggg
     7981 catgaaatga cgcacgccct gcatgaacac ggtaaaaata aggtcgggca gcaaatcttg
     8041 accaatacgg cggcgcagat aggcacgcag attatattag acaaaaaacc ggatactaat
     8101 ccggaattgg tcggattggg tatggatatt ttggggacgt acggtcttac cttgccttat
     8161 agccgcagct tggaagaaga agccgatgag gggggaatga tgttgatggc gcaggcaggc
     8221 tatcatccgg cggccgctgt cagggtttgg gaaaaaatga atcaggaaaa cgaccaaaac
     8281 ggctttattt atgctattac ctctactcat ccgacaaaca atgcccgtat agaaaatcta
     8341 aaacggttgt tgccgaccgt tatgccggtt tatgagcaaa gtgtcagaaa taaggggcgc
     8401 gttaataaaa aacgtcggcg ttaagggaag gggatcgaga atttgagcca ttgtttcaaa
     8461 atgccgtctg aaatcgtttg agattcagac tgcatttaaa cagttctgtg ccccacccgt
     8521 tttgccttca agcccgcgcc gcttaaatcc aatccccccc tgcaacgggc gcaaaaaatc
     8581 cccgtacctc gcggtcggtg atgtttcggg tttcgcgccc gtctgcgccg tgccttgctg
     8641 tcgggctatg tcgtctgcac cgccgccgtt tcggcttggt ttgatgcggt cgggcattta
     8701 tgcggctacg ccctgatatt cagttcggcg gtcttgcctg tcttggcagg catattcagc
     8761 agcgcgtcta ttgtgaactt gctgattttg ccgtttgcca agtcggctac gcaatggctt
     8821 cgatgccctt gtttttgacg atggtcagca gcttcaacgc cgcgccgctc ggctttttaa
     8881 cgccccgctc ccaagccgaa acgtgatttt ttcccacgtt gagatagatg gcgaaagcgg
     8941 cttgcgatag tgcctccttc tccctgattg ccttgatgtc tccgccgctc aacggtttga
     9001 tttcggtcag gcaggacttg tcaaagtcgc gcatggtctt tttgtcgatt gcgccgatgt
     9061 tgtaagcccc ttccatcatt tcatgaatgg cggctaatgc ctcgtttttg tatttcatga
     9121 ttctatctcc gtgataatgc cgtcttcttt caaagccgcc agctctgccc gcgtgtattt
     9181 cagataatat gcggcggctt ttcggtaaac gtcaagttct ttatccgaaa tgttttccct
     9241 gtcgttcttg gcaaaggcgt aaacaaaaaa tgccttgtct gcctgtttga acagtatcag
     9301 actgcgataa ccgccgcttc tgccttgtcc ttgccttgct atgcgctgct taatcacacc
     9361 gccgcctaaa tctgcgtcta tcagcccgtt atccgcccgc tctaccgctt ccagcagctc
     9421 ggaatcgttg attttgtgct tcttggcaaa tttcactatc cattggtttt tgaatatccg
     9481 cattgtgtag ccgttgatta gtgatacttg gaattatacc agagacagac aacacggcaa
     9541 gctgcctttt ttatgccccg caatttctcc gtttatgccg cctgaaacac tggcggctaa
     9601 cctgattatt tttttaatca atcgctttta atggctcaaa ttgcgttttt agcgcgtttc
     9661 gctgtcttcc tattccccgt atataaccaa gcggtttaac cgccattcct gctcaaaata
     9721 ggcgcggttc cgtgcgtttt ggcttttcat tccgctacgc tcccactgcc aagacggtga
     9781 aaaaccggaa aaccccaaaa gttgttaccg attcgttacc atttgttacc gcatttgtta
     9841 ccaatttttt atctttttct tat
//
LOCUS       DAWIVB010000059         9844 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWIVB010000059 DAWIVB010000000
VERSION     DAWIVB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.059"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(62..676)
                     /locus_tag="WIX60_001048"
     CDS             complement(62..676)
                     /locus_tag="WIX60_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ5305329.1"
                     /translation="MNFTRLLNQVLSTVQKKGGTFSDSPLNSFGGGALVAGIASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAKRRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
     gene            complement(713..2863)
                     /gene="dinG"
                     /locus_tag="WIX60_001049"
     CDS             complement(713..2863)
                     /gene="dinG"
                     /locus_tag="WIX60_001049"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ5305330.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            2966..3190
                     /locus_tag="WIX60_001050"
     CDS             2966..3190
                     /locus_tag="WIX60_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305331.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLMIESEKNGRY"
     gene            complement(3258..4559)
                     /locus_tag="WIX60_001051"
     CDS             complement(3258..4559)
                     /locus_tag="WIX60_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ5305332.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(4549..5460)
                     /locus_tag="WIX60_001052"
     CDS             complement(4549..5460)
                     /locus_tag="WIX60_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ5305333.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAAGALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            5568..5966
                     /locus_tag="WIX60_001053"
     CDS             5568..5966
                     /locus_tag="WIX60_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ5305334.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            6015..6620
                     /locus_tag="WIX60_001054"
     CDS             6015..6620
                     /locus_tag="WIX60_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ5305335.1"
                     /translation="MTRQSLQQAAESRRSVYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            6806..8915
                     /locus_tag="WIX60_001055"
                     /pseudo
     CDS             6806..8915
                     /locus_tag="WIX60_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            9030..9725
                     /locus_tag="WIX60_001056"
     CDS             9030..9725
                     /locus_tag="WIX60_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ5305336.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         2128 a         2538 c         2885 g         2293 t
ORIGIN      
        1 cgttttgagg ccggggtcgg aataaccgtt ttttgatgat tttccctccc cggctgtgtc
       61 atcaaaaccc caattgcctt tccaaactct ccaccagatt gtcatccagt ttcaaagcct
      121 gcgacaggcg ggcgaggaag acggtttctt tccgcgacaa atcggcacag accaaccttg
      181 ccgccagata ggcctccgcc gccaacgcct catcgttgcc gacggcggcg gcgatgtctt
      241 cgatgcttgc gggcaggcgg tattcggcgg cgagccatgc ggcagtttcg gggtctgtgc
      301 cgctttcctg ttcgatagtc cggcgtttgg cttcgtctat catgccgtct gaagcggcgg
      361 cggctatcat ggtgcgcaat acggtgcggc tgtatgtttc ttcagtttct ccggcaggtt
      421 ggaaatcgct ttgtgttaca gttgcccgcc ctttgttttg ctgccacatc tgatagcccc
      481 ggtaggcgag gtagcccaaa gcggcggtcg aaccgatttt ggtaatggtt ttgcggtttt
      541 taccgttcag cagcatggag gcgataccgg caaccagcgc gcctccgccg aatgaattga
      601 gcgggctgtc ggagaatgtg ccgccttttt tttgaaccgt gcttaagact tggttgagca
      661 gtcgggtaaa gttcatgaat ttttcctttc tgttgcggga agggcggtat gtttacccta
      721 tccttttaaa cggcggcagg ccggccaata attgttggcc gtaccgctgt gttttgatgc
      781 ggttgtcgag gatggttacg cggccgtagt cttgttcggt gcggatgagg cggccgacgg
      841 cctggatgag tttgatgccg gcttcgggga cggtgatttc aataaaagga ttgccgccgc
      901 gctgttctat ccagcggttt tgggtttttt cgatggggtt gtcaggcatc gcaaacggca
      961 gcttggcgat gatgacttgc acgcaggcgg tgccgggcag gtcgagtcct tcggcgaaac
     1021 tgtcgagtcc gaagatgatg ctggctttgc cttcttctat ggcccggtgg tgtttttgca
     1081 ggaggacggc tttgggtaat tcgccttgta cgagcaagag cggcaggtag tctccgggca
     1141 ggcgcagggc gacatcctgc atttgtttgc gcgaggaaaa caagacgagc gtgccgatgg
     1201 cttcggtggg cgaaataagc ttgggcagcc attcgatgac ggcggcggtg tgggcttcgg
     1261 ggtctttggg gctggcgtat atggggggga tgtagagttc gccctgtttt tcaaagtcaa
     1321 aggggctttt gagggcgagg gtggtggttt cgggcagcca ttgcagcccg gtttggcgca
     1381 gcatcaggtt gaagttgccc aaggattgca gggtggcgga agtcaatacc gcgcctgccg
     1441 cacgccgcca caggctgttg gcaaggtggg atgcgctgct gatggggctg gcgttgaaaa
     1501 tgtagtcgtt tttgtcgtcg gcacggcggg ttatccattt tgccagcggc tcttcgcctt
     1561 cgagggggac ggtggagagc aaatcccaaa ccgcgctgat ttgttcgata cggacgatga
     1621 agatgccgaa ctcgccggtc aggcggtcga ggagcgcgcc gtcctgttct ttttcgcggc
     1681 gcgcggcgga aagcgcatcg ttcagcccga caacgtgttt gagcaggctg cgcgcagcaa
     1741 tggccgtatt ggaaacggtg gtttcgaggc cttcggggat tttgccgtct tcccacagcc
     1801 aagtcggttc gctgttggtt cgtctgtcgt tttcagacgg ccccatactt aaagacggct
     1861 cttccgccaa atggaactgc cattcgtgca ggctgtcgag caaggatgcg gcggcttcgt
     1921 cggccaggtt ggcaagttcg gctttatcgg tcagcgcggc aattttgccg gtcagctgcg
     1981 gcagtttttc cagcgtccaa acggcaatat tccatgaatg ttcggcggca aaacggctga
     2041 gggctttttt gggcaggtgg tgcgcttcgt ctatgcaata gaaactgttt tcgggcgcag
     2101 gcagaatcac gccgccgccc atactgatgt cggcaagcag aagatcgtgg ttggcaacga
     2161 cgacatcgac ggtttccaag acatcgcgtg ccaggtaaaa cggacattcc ggacggttgg
     2221 gacaggcggt tttcaggcag ccgtggcggt cgttggtcac tttgagccaa atcgcgtcgt
     2281 cgattttttc cggccaggcg tcgcggtcgc cattgaaccg tcgggcagaa aattcgtcgg
     2341 caatgtcgcg cagcagcttc aattcttcgg gcttgggttt gctgtcccac aagacggcgg
     2401 gggcttcaaa gccgagcagg ttttgctggg cattgctttg cgtcagttga tagagtttgt
     2461 aggggcagag atagcggccg cgccctttgg caagcgcgaa gctcagttcc aaaccgcttt
     2521 tttcgaccaa aaacggcagg tcgcggtcta ccaactgctc ctgcaaggca accgttgcgc
     2581 tgctcacaat cagccgcttg ccgcgcgtct gcgccatgat gccgcccgcc agcaggtagg
     2641 caagcgattt gcccacgccg gtcggccctt cgattacggc gatgctctcg ccctcgcgct
     2701 tgggcggctc gccgccttct tcgcgcgcca acgtccgcga aaaagcgttg gcaaccgccg
     2761 caatcatttc ccgctgcgaa gcgcgcggac ggaaaccggg caggtttttg ccgatgtttt
     2821 ggtaatggtc gcggatggcg tttttttcta aatcggtgag catggcgttt tgtacggcgg
     2881 tagaagtggg cttattttaa cattgcacgg aagcggtaca atatcgttgt cggaatgggg
     2941 ggtgaggtga atcgtgcgga cgtggttggt tttttggttg cagcgtttga aatacccgtt
     3001 gttgctttgg attgcggata tgttgctgta ccggttgttg ggcggcgcgg aaatcgaatg
     3061 cggccgttgc cctgtgccgc cgatgacgga ttggcagcat tttttgccgg cgatgggaac
     3121 ggtgtcggct tgggtggcgg tgatttgggc atacctgatg attgaaagtg aaaaaaacgg
     3181 aagatattga gtcattcgga cgcaatgccg tctgaaacgg aagttcagac ggcatttgtt
     3241 ttaggttgcc gtaccgctta gggaataccg gcgacaggat gggcgggata gccgtgggta
     3301 tcgaccgaac aggcaaaccg ccaaggcgtg tggacggtgt cggcggacag gtgggcgagt
     3361 tcgggaatgt gccgtttgat aaaagtaccg tccgggtcgg tacggtatga ggcggcggca
     3421 atgtcggggc aggtgtgccg tgaggcggca agccaccagt tgccttggtt gattgccgca
     3481 tcgaaatcgg tcagctgccg ggcaaaccat atctcgcctt cgcggcgggg gaggtttaaa
     3541 acgtggcaga aaaaatccgc gctcaagcgt ctcagggcgg ggtggaggct gccggttttg
     3601 tgcaaacagc gcatcgcggc atcgataatc ggaatgccgg tccggccctg ctgccaaagc
     3661 gtcaggcgca gggtgtgttc aggattgccg tctgaagggt cgtcatccgt gtgctgcaag
     3721 gcaagttgaa ggaaaaaatc gcggcggatg atgttgtccg cccacgcgtt cagacggcgt
     3781 tcgaggcttt cccgcgcgag caggcgcggc gagatgcagc cggcactcaa atacgcgccc
     3841 atcagcgaag tgtgtttgcg cgaggggaaa tcctttaaaa cggagtagga atccgcctgt
     3901 tcgagaaacc gccgccactg ccgccaagcc gccgtttcgc cgctgttttg cggcaggaag
     3961 atgccgtctg aaagggcggc aggcggcggg gcggaaaggt tttcggggaa gggttggcgg
     4021 tatgccgcga ataggtccgg accggcgggg ggctgcttgg aaaagcggtc gagccatact
     4081 tcgcggtagc ggtcgaaatc ggtgtgtgcc gtgccgccgt cgggtatcag gtcggttttg
     4141 ccgaaaacgg cgcggtcatt gacgaaggtt aacgcgatgc cgtgtttgtc caattcatgc
     4201 caaagggcgt tgtcggcgag tttgtcggca aaagtatggg attcgtcggc gatgacggcg
     4261 cggatattga ggcggacggc gagccggacg agctcggcag gcgatgccgc cgtgtagagc
     4321 gggatgccgc gccctgcaag cccttgggcg agttcggcgg cggattggcg gtagaacgcg
     4381 gcgcggcgag ggttgtctgt ttcggcatcg tcaatccaaa taccgataac gggcaaacct
     4441 tcggcaacgg cggtgcataa ggcggcgttg tcgcggatgc ggaggttttg gcggaaccag
     4501 acgagcgtgt gtgcggcgca cgtgtccgca taaagggggc gggcggtttc agacggcatt
     4561 tcggcagcct ttcctgctgg cgattttttc gttcagaaaa tcgatgaagc tgcggacttt
     4621 cgcgcttaag aatgccctgt ctgcataaac ggcattcagc cggtcggtcg ggacggcgta
     4681 tccgggcagc agcctcacca gcgtgccgca gcgcaaatcg tgttccgccg cccaaagcgg
     4741 ctgataaccg atgcacgcgc ccgccttaat catttcgcgc atcatcagcg tgttgtcggt
     4801 acggatgacg ggggtcagtt caagccggta ttttttgccg tccgatttgc gggtgaggtc
     4861 gagtttctgc tggttggtgt aggtcggcag gacggcgggc agccccgcca cttcttccgg
     4921 cgtttccggc acgccgttgc gcctcaggaa gtcgggcgag gcaagcaggg caaattcgat
     4981 ttccgccagt gggcgcgcaa tcagtgacgg ggacagggtt tgggaaacgc gcaacgccaa
     5041 atccacgcct tcggcaatca aatcgacgtg gcggttgtcc aaaatcagtt ctaatgtcac
     5101 ttcgggataa cgttcgcggt attccgccag ccagttgcat atctggctgc cggcaaacca
     5161 cagtggcatc gttacgcgca gcagcccctg cggtttttcc gtccccccgg cggctttttg
     5221 cgcggcatcg tcgagcgtgt cgagcgcgta actgcattgc cggtagtatt cttccccggc
     5281 ttcggtcagg ctgaggttgc ggctgttgcg gtgcaggagt ttggcttgga cggtgttttc
     5341 caagtggctg acgtgtttgc ttgccattgc ggtggagatg ccgagcgcgc cggcggcgcg
     5401 ggtgaagccg ccgctttgga cgacttggcg gaaaaccttg aggctgaaca gggtgtccat
     5461 attttcttgt gtggaaaagt tgtatcaata aaagcagtat atatttgaaa aggggaaaca
     5521 tctatactct accgcctgaa atgaagacaa atatcaaagg agcttttatg tccgattgct
     5581 gcaaccgtat ccaaccggtt ttgctttctg ttttgcgtat cgtaaccgcc tacctgtttt
     5641 tgttgcacgg tacgtcgaaa atcttcgcct tccccattga aatgggcagc ggttcgcccg
     5701 gcgggctgtt gctgcttgcc ggtattttag aaattgtcgg cggcattttg ctggtgttgg
     5761 gcctgtttgc gcgccctgcc gcgtttgttt tgtccggtca gatggcggtt gcctatttta
     5821 tggcgcacgc ttccggaaat gctttgttcc cgattgccaa cggcggcgag tccgcagtgc
     5881 tgttctgctt cgtattcctc tatatcgcgg cagcgggcgg cggagcatgg tcgctggaca
     5941 ggctgttttt caagcgtaaa gcctgaaccg gactgcctaa agtgtatttt gttgaatgtt
     6001 tttgaggaaa agaaatgacc cgtcaatctc tgcaacaggc tgccgaaagc cgccgttccg
     6061 tttattcgtt aaataaaaac ctgcccgtcg gcaaagatga agttgtccaa atcgtcgaac
     6121 acgccgtttt gcacacacct tcttcgttca attcccaatc tgcccgcgtg gtcgtgctgt
     6181 ttggcgaaga gcatgataag gtgtggcaat ttgtcgaaga cgcgctgcgt gccgtcgtgc
     6241 ctgccgacag ttttgaaccg accgcgcaaa aattgaacct gtttaaggcg ggtgcggcaa
     6301 ctattttgtt ttatgaagat caaaatgttg tcaaaggttt gcaggagcag ttccctgctt
     6361 atgccgccaa ctttcccgtt tgggcggacc aggcgaacgc tatggtgcag tatgccgttt
     6421 ggacgacact tgccgcggtc ggcgtaggtg caaacctgca acattacaat cccttgcccg
     6481 atgcggcgat tgccaaagcg tggaatatcc ccgaaaactg gttgttgcgc gcacaaatgg
     6541 ttatcggcgg tattgaaggg gcggcaggtg aaaagacctt tgaacccgtt gcagaacgtt
     6601 tgaaagtgtt cggcgcataa tttcgcggtc aaaaaaatgc cgtctgaacc ctgttcagac
     6661 ggcatttttc agtatcaggc ggcgagtttt ccgcattctg agacctttgt ttacaaatat
     6721 catgttcaat atagttaaaa gaaattattc tcatttcctc cgtgaggcaa tataattcgg
     6781 ttgttttgtt aaattgagta taaaaatgaa aatatcattt catttagctt tattacccac
     6841 gctgattatt gcttccttcc ctgttgctgc cgccgatacg caggacaatg gtgaacatta
     6901 caccgccact ctgcccaccg tttccgtggt cggacagtcc gacaccagcg tactcaaagg
     6961 ctacatcaac tacgacgaag ccgccgttac ccgcaacgga cagctcatca aagaaacgcc
     7021 gcaaaccatc gatacgctca atatccagaa aaacaaaaat tacggtacga acgatttgag
     7081 ttccatcctc gaaggcaatg ccggcatcga cgctgcctac gatatgcgcg gcgaaagcat
     7141 tttcctgcgc ggttttcaag ccgacgcatc cgatatttac cgcgacggcg tgcgcgaaag
     7201 cggacaagtg cgccgcagta ctgccaacat cgagcgcgtg gaaatcctga aaggcccgtc
     7261 ttccgtgctt tacggccgca ccaacggcgg cggcgtcatc aacatggtca gcaaatacgc
     7321 caacttcaaa caaagccgca acatcggtgc ggtttacggc tcaagggcaa accgcagcct
     7381 gaatatggac attaacgaag tgttgaacaa aaacgtcgcc atccgtctca ccggcgaagt
     7441 cgggcgcgcc aattcgttcc gcagcggcat agacagcaaa aatgtcatgg tttcgcccag
     7501 cattaccgtc aaactcgaca acggcttgaa gtggacgggg caatacacct acgacaatgt
     7561 ggagcgcacg cccgaccgca gtccgaccaa gtccgtgtac gaccgcttcg gactgcctta
     7621 ccgcatgggg ttcgcccacc ggaacgattt tgtcaaagac aagctgcaag tttggcgctc
     7681 cgaccttgat acgccttcaa cgacaaatgg cgtgcccaat ggcagctcgc ccaccgcacg
     7741 gcggcgcagg attttgatca tttctatgca ggcagcgaaa atggcaactt aatcaaacgt
     7801 aactacgcct ggcagcagac cgacaacaaa accctgtcgt ccaatttcac gctaaacggc
     7861 gactacacca tcggccgttt tgaaaaccac ttgaccgtag gcatggatta cagccgcgaa
     7921 caccgcaacc cgaccttagg ttacagccgc gcctttactg cttccatcga tccatacgac
     7981 cgagcaagct ggccggcttc gggcagattg cagcctatcc tcacccaaaa ccgccacaaa
     8041 gccgactcct acggcatctt tgtgcaaaac atcttctccg ccacgcccga tttgaaattc
     8101 gtcctcggcg gccgttacga caaatacacc tttaattccg aaaacaaact caccggcagc
     8161 agccgccagt acagcggcca ctcgttcagc cccaacatcg gcgcagtgtg gaacatcaac
     8221 cccgttcaca cactttacgc ctcgtataac aaaggtttcg cgccttatgg cggacgcggc
     8281 ggctatttga gcatcgatac gtcatcttct gccgtgttca acgctgaccc cgagtacacc
     8341 cgccaatacg aaaccggcgt caaaagcagt tggctggaca atcgtttgga caccacattg
     8401 tccgcctacc aaatcgaacg cttcaatatc cgctaccgcc ccgacgcgga aaataatccc
     8461 tacacttggg cagtcggcgg caaacaccgt tcgcgtggcg tggaattgtc cgccatcggg
     8521 caaatcatcc ccaaaaaact ctatctgcgc ggttcgttgg gcgtgatgca ggcgaaagtc
     8581 gttgaagaca aaaaaaatcc cgaccgagtg ggcatccatt tgaataatac cagcaacgtt
     8641 accggcaacc tgtttttccg ttatacccga ccgaaaacct ctacggcgaa atcggcgtaa
     8701 ccggtacagg caaacgctac ggttacaact caagaaataa agaagtgact acgcttccag
     8761 gctttgcccg agttgatgcc atgcttggct ggaaccataa aaatgttaac gttacctttg
     8821 ccgcagccaa tctgttcaat caaaaatatt ggcgttcgga ctctatgccg ggtaatccgc
     8881 gcggctatac tgcccgggta aattaccgtt tctgatgaaa tcaggcaaag gctgaaataa
     8941 aactaaacac attttttcac tcaaatcgaa cacgccttca ataaaatgcc ataaaatccg
     9001 cacattaatc tgacacacaa gagataccta tgaaactgaa aaccttagct ttgacttcat
     9061 tgaccctgtt ggcattggcc gcttgtagca aacaggctga aaccagtgtt ccggcagaca
     9121 gcgcccaaag cagctcatct gctccggcag cccctgctga gttgaacgaa ggtgtgaact
     9181 acactgtatt gtctacgcct attccgcaac agcaggccgg taaaatcgaa gtattggaat
     9241 ttttcggcta cttctgcccg cattgcgccc atcttgagcc ggtcttgagc gagcacatca
     9301 aaacgtttaa agacgatacc tatatgcgcc gggagcatgt cgtgtggggt gatgaaatga
     9361 aacctttggc acgtttggcg gccgcagtgg aaatggccgg tgaatcagat aaagccaaca
     9421 gccatatttt cgatgcgatg gttaatcaaa aaatcaatct ggccgatacc gataccctga
     9481 aaaaatggct gtccgagcaa acagcgtttg acggcaaaaa agtattggct gcatttgagg
     9541 ctcctgaaag ccaagcgcgt gcggctcaaa tggaagagtt gaccaataaa ttccaaatca
     9601 gcggcacacc gactgtgatt gtcggcggca aataccaagt tgaatttaaa gactggcagt
     9661 ccggtatgac cacgattgac cagttggtgg ataaagtacg cgaagagcag aaaaagccgc
     9721 aataagttga ggattgaatg agtaaaggcc atctgaaaat aggatttcag acggcctttt
     9781 gtatttaggc tttatagaag agatgattgc ttaaagcctt atggttttaa atcagaatat
     9841 atag
//
LOCUS       DAWIVB010000060         9794 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWIVB010000060 DAWIVB010000000
VERSION     DAWIVB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.060"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(10..1305)
                     /locus_tag="WIX60_001057"
     CDS             complement(10..1305)
                     /locus_tag="WIX60_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ5305337.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAVDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLTYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNVVYAVNTYAGLNPIFRPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPERMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1431..2318)
                     /locus_tag="WIX60_001058"
     CDS             complement(1431..2318)
                     /locus_tag="WIX60_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ5305338.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2318..3283)
                     /locus_tag="WIX60_001059"
     CDS             complement(2318..3283)
                     /locus_tag="WIX60_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ5305339.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WIX60_001060"
     CDS             complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WIX60_001060"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ5305340.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4618..4725)
                     /locus_tag="WIX60_001061"
                     /pseudo
     CDS             complement(4618..4725)
                     /locus_tag="WIX60_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WIX60_001062"
     CDS             complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WIX60_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ5305341.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WIX60_001063"
     CDS             complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WIX60_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ5305342.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WIX60_001064"
     CDS             complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WIX60_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ5305343.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPEILLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPQQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WIX60_001065"
     CDS             complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WIX60_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ5305344.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8694..>9794
                     /locus_tag="WIX60_001066"
     CDS             8694..>9794
                     /locus_tag="WIX60_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ5305345.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIISVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2512 a         2611 c         2422 g         2249 t
ORIGIN      
        1 ctgcgacgct taacggctgt ctttccactg ataatatttc gagccgagga aagaaccgag
       61 tttgcgcaaa aacggttgca ggataatatt cggctggcgc aaccgctcaa acggcttcag
      121 acggcattcg tcccctaaaa ttgcttcggc aaccgccaga cctgcaatgc ctgttatcgc
      181 catcccgtgt ccggaataac cttgcgcata aaaaacattc ggggctaaac gtccgaaatg
      241 cgggacaagg ttggcggtaa tgtcgcactc cccgccccac gaatattcga ttttgacatc
      301 ggcaagctgc ggaaaaactt taagcatatc ttggcggaca agctcggtca tacgctcagg
      361 attgtcgata aactcgttat ccttaccgcc gaaaagcagt ctgccgtccg cgctgaggcg
      421 gtaataatcc aaaatatggc ggttgtcgca tactgccata ttgttacgga taagcccttt
      481 tgcgcgcgcc cccaagggtt cggtcgcaat aataaaggtg ctgacagcaa tcgccttgcg
      541 ttccaaaggc cggaatatcg ggttcaaacc tgcataagta ttgacagcat agaccacatt
      601 tttgcactcg acgctgcctt cgggcgtgta aaccagccaa ccgttttgat gcggttcgat
      661 gcacgtcatc ggggattgct cgaaaatctg cgcaccggct tcggcagcgg cacgagcgat
      721 gcccaaagtg taagtgagcg gatgcaggtg tccggataag gggtcgaatt gtgccccttg
      781 gtacatatcg ctgtcaagct gctgtttcaa ctcggcttta tcccaaagtt gataatgact
      841 cgcaccgtaa tgccgttggg cgtgttcatg ccactgctgc aactcttccc aatgctgcgg
      901 acggacggca accgtggcat aaccgcgctg ccaatcacaa tcgacggcat gtttgcggac
      961 gcgttcgtcc accagttcga ccgcctgcaa agactgttgc caaaaccatt gcgcctgctc
     1021 caagccgacc tgtttttcaa tttcccccat accgcaggcg taatcgctga taacctgccc
     1081 gccactccgt cccgacgcgc cgaaaccgat acgcgcggct tccaacacaa ccgtttcatg
     1141 tccctgctcc gccaagggca atgcagtgca caaaccactc aatccgccgc cgatgataca
     1201 ggtatcggtt ttcagacggc attgaagttt cggataaaca gtatgaggat taaccgaact
     1261 gaaataataa gaaggcagat attcttgaaa atcagggcga atcattgtgt ttgctttatc
     1321 aggtgtattt tcggacggaa tgatacaggc tgtcgggcca tatcgtccaa acagaaaatc
     1381 ggttgaagaa aacaggctga cccagtcatg cggtcagcct gccttattaa ttaatttgct
     1441 ttctcggcag ccaatttttc ctggcggtag gcttctgccg cttctcggtc acgcttggtt
     1501 gcctgcctca tcatccaata attgacgatg atgaccaatg ttccgatgat gccgattagg
     1561 atggtcgcca agacattcat ctgaggatcg agacccaact tgattttgga gaaaatcacc
     1621 tgcggcaatg tggatgaacc ggggccggag aggaatgagg taatcaccaa atcatccaaa
     1681 gacagggtaa tgccgagcag aaagcctgaa gcgatggcag gggcaatcaa aggcaaagtg
     1741 atgacaaaaa agattttcag cgggcgcgcg cccaaatcca ttgcggcttc ttcgagcgac
     1801 tggtcaagct caaccagacg cgaacggata acaacggtaa tgtacgccat acacagcgtc
     1861 gtatgtccga ggaagatggt gaaaaagcca cgatcgaagt agagatgttg taaccattcg
     1921 ctgccctgca aaaatatctg tacctgaata atcagcagca gcatagacag accggtaatc
     1981 acgtcgggca tcaccatagg tgcggaaatc atgccagcga acaaggtact gccgcgaaaa
     2041 cgtttaatcc gcgccatcgc atagcctgcc agcgtgccca aaacgacggc ggcaagcgaa
     2101 gacacaacgg caatccgcag cgacagccaa gcggcttcca agatggtgtc gttttccagc
     2161 aatgcgccgt accacttggt cgaaaagccg ccccaaacgg ttaccagctt ggattcgtta
     2221 aacgaataga tgaccaaaac aaccagcggg atatacagaa acgccagcga cagtgccaac
     2281 atcagtttca agaaccaaga taatttggat ttctgcatta tttggctcct tcttccaatt
     2341 cgcggttttc ataatgctga aacagggcaa tcggcacgac cagcagcgcg accatcacga
     2401 cggcgacggc ggaagccagc ggccagttgt tttgatcgaa gaacgcctgc cacaagactt
     2461 taccaatcat caggttttcc gaaccgccga ccagctcggg aatgacgaac tcgccgacag
     2521 cagggacgaa aaccagcatg gagcctgcaa taatgccggt tttcgacaaa ggcagggtaa
     2581 tcgtcaagaa cgatttgacc ggccccgcgc ccaaatcgga agccgcttca agcaggcggt
     2641 tgtcgagttt caccagttgc gtgtatagcg gcagaatcat aaacggcaga taggcgtaaa
     2701 ccatcaccaa attgagcgaa aaggcattgt agaacaaatc caaaggctcg ctgataatac
     2761 ccattttaat caacaggttg tttacaatgc cgttatgccc gagcagaccc atccacgcat
     2821 agacgcgcaa caggaacgat gtccaaaagg gcagcataat ggcaagcagc aaaccattgc
     2881 ggacagaagg attggcacga gaaatcgcat aggcggtcgg ataaccgacc aacagacaaa
     2941 ttaccgtcgt agtcagcgca gtcttaattg aagaccaata agtcatcaga tagatattgc
     3001 tgttttcacc gtcgccgaac ggattgagcg tactccaaaa attttggaag atgtctgcat
     3061 aattttggta gctgacagca atattcagac gacccaaatc ctcatctatc gtcgttaaag
     3121 gagtaaacgg cgggatggcg atttcttgtt cggcaaagct gattttcagc acgatggcga
     3181 acggaatcag aaacagcacc aaaagccaaa tatacggtac ggcaatcacc gcacgctgcc
     3241 ccggacggcg gaacagtttg tttttcagtt tattaaggtt cattgcattc cccttaaatc
     3301 aacggaacaa cggagtcggt tggttttccg gccagctgat atagacggtt tcgtcccaag
     3361 tcggcggtgt aatgttgcgc acataccagt aaggggcggg gacttggctt ttgacgacgc
     3421 gcccgttgcc gagcttgata tggtaaatgg cgaagctgcc caaataggcg atttctttta
     3481 ccgtgccttt cgcccagttg tagtcgccca aatattcggg tttttcttta tataaatcaa
     3541 tatcctctgg tcgaatacta acccaaaggt cctgctcgct cggaccaccc aaaccgtgat
     3601 cgatgcggac gtggttttcc aaaccttcgc attcgataac ggcatagtcg gcatgatctt
     3661 caatcaccac accgtcaaag atgttggttt caccgataaa ctcggcagtg aagcggctgt
     3721 tgggatagtc gtacacgtcg ctgggtgtgc cgacttgctg caactgaccg tcagacataa
     3781 tggcgatgcg ggtcgccatc gtcatcgcct cttcttggtc gtgcgtaacc ataatacagg
     3841 ttacgccgac ttgttccagc gtattgacca actcaagctg ggtttgttgg cgcagttttt
     3901 tgtccaatgc accgaggggc tcatccagca gtagaatttt cggacgtttt gccagactgc
     3961 gtgccaaagc aatgcgctgc tgctgaccgc cggacaattg gtgcggtttg cgtttagcaa
     4021 atttggtcat ctgaaccagg cggagcattt cttcgacgcg cgcggcgatt tcgcctttag
     4081 gcattttgtc ctgtttcaga ccgaaggcaa tgttttgttc tacggtcata tgcggaaaaa
     4141 gcgcgtaact ttggaacatc atattgatgg ggcgatcata gggtgcaagt ttggtaatat
     4201 cctgaccatc aaggataatt tttccctgat tgggactttc catacccgcc agcatacgca
     4261 gcagtgtaga ttttccgctg ccggaactgc ccaaaagggc gaagatttcg tgttgataaa
     4321 tgtccaagtc gatgttatcg acagcgtaat tgtcaccaaa ctttttcacc aaaccttgga
     4381 ttttgagata aggtttggct gaagacgcag tggttgcggt cataatggca atactccaat
     4441 aaaaagacga gtaccggcaa aacggatttt cgaatgggtg ataaaaagct gtttgattgc
     4501 tggcgggagt taaacgtttg atgccgtctg aaactcttgt aaagcgcacg ggcagcatga
     4561 aatggaacaa gattccaaag aactttatat tatattagtt tatgcggttt tcgggcaata
     4621 tagtggatta aatttaaacc agtacagcgt tgccttgcct tgccgtacta tttgtactgt
     4681 ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctataaatat ggatttgagc
     4741 ttgtcggaaa gcaacagaaa agaaaacacc gcccattttt ctgggcggtg tcggaaagcg
     4801 taattattta cgcagaccca agcgggtaat caacgcgcga tacgtatcgg gctgggtacg
     4861 gcgcaagtag gccagcaggc ggcggcgttg gctgaccatt ttcaacaggc cgcgacggct
     4921 gtggtggtct ttggggttgg ctttgaagtg gggggtcagg tcgttgatgc ggaaagtcaa
     4981 cagagcgact tgtacttcgg aagagccggt gtcgccttct ttgcgttgga aatctttaac
     5041 gatttgtgct ttttgttcta cggtcagtgc cataatgaaa actccaaaaa tataagaatc
     5101 cccataggga ttcagacaag tttgccaagc ctgaagacaa cggcaaactc cctatgccca
     5161 agataggaca acgtggcatt atgacacaat tcccgcattt ctgcacaata ttttaagacc
     5221 gtggaagtaa ccgttaaaaa tgccgtctga agcattgtct gcttgagacg gcaatgttca
     5281 gacggcatat gcgcttcaaa ccacgatttc ttctttcttc ttcggttctt taccgatatt
     5341 gtcgcggctc aaaccgaaca ttagcagaag cgggctggca accaatacgg aagaataaat
     5401 gccgaacacg atgccaatgg tcaacgccat agaaaagccg tgcaaggccg caccgccgaa
     5461 caccagcatg gatacgacca tcgcctcggt cgaaccgtgg gtaatgatgg tgcggctcat
     5521 cgttgcggta atcgcgttgt cgatgacttc cggcacggca tgtccgcgca tcgccggctt
     5581 gcggaagttt tcacggatac ggtcgaagac gacgacggat tcgttcacag aatagcccaa
     5641 tacggcaagg atacccgcca agacggtcag cgaaaattcc cattggaaga aggcaaagca
     5701 gccgagaata atcacgatgt cgtgcatatt ggcgataatg gcagatacgg caaaacgcca
     5761 ttcaaaacgc atcgacaggt aaataatgat gccgataacg acaaaaccta aagccatcaa
     5821 tccattactt accaattcct caccgacttg cgggccgata aattcgactt ggcgcaaggt
     5881 aacgtcggga ctgtcttttt tcagcaaatc cataacctga ttggacaact gtgcggaagt
     5941 aacaccttct ttgttcggca ggcggatcat gatgtgtttg ttcgtaccca atgcctgaac
     6001 ctgtacatca cctattttca gcgtatcgag gcgttcgcgc atcttattga catccgcacc
     6061 ctgctgatat tggacttcca ttaccgtacc gccggtaaat tcgacagaga aattcagacc
     6121 tctggtaacc aaaaagaaca cggcagcgat aaacgtaacc aacgaaatga aggtcgtcag
     6181 tttgccgtag ctcataaacg gaatatcgcg tttgatttta aagagttcca tagcttactc
     6241 cttgcctcct gccatttcgg ctttcggctt ccacaccgaa ccaatggaaa tattctgcaa
     6301 tttgcgtctg cgtccgtacc acagattgac caacgcacgg aatacgacga cggatgaata
     6361 catcgaagtc agaataccca aacagtgtac gaccgcaaaa ccgcgtaccg ggccggaacc
     6421 gaataccaaa agcgcgatac cggcaatcag cgaagtcagg ttcgaatcga caatggtcgc
     6481 ccatgcgtgt tggaaaccga gattgattgc ctgctgctgc ggcacgccgg cacgcaattc
     6541 ttcgcggata cgttcgttaa tcaagacgtt ggagtcgatt gccataccca aagtcaacgc
     6601 cagcgcggcc atacccggta acgtcaacgt tgcctgcatg gcagacaaaa taccgattag
     6661 gaacagtatg ttggcactca atgcaatggt agaaaagaaa cccatcagac gatagtaaac
     6721 caccatgaat gcagcaacga tggcaaaacc ccataaagtc gaatggaagc ctttttcgat
     6781 gttctcctta cccaaagacg gaccgatggt acgttcttcg acaatctgca tcggtgcggc
     6841 aagagaaccg gcacgcaaca gcaaagacgt atcattggct tcggctgtcg tcatgcttcc
     6901 ggaaatttcc acgcgtccgc cggtaatggc agtacggata accggcgcgg ttacaacctc
     6961 ggattttcct tggtcgatca aaaccatcgc catgcgtttg ccgacatttg cggcagtcag
     7021 ttcgccgaaa atgctgccgc ccgcgctgtc caagctcaga ctgacggcag gtgcgcccat
     7081 ttggtcgaaa ctcggttgcg catcgttgat gttgtcgccc gtcagctcga cctgtttgct
     7141 gatcagcaga atttcgggac gatctccgcc gcttgaaagc agctcataac cgctcggcac
     7201 gttgccttcc aatgcctcgc gcaacttggc aggatcgtcc tccaccatac gcaattccaa
     7261 agtcgcggta cggccgatga tgtcttttgc cttggcagta tcctgaacgc ccggaagctg
     7321 cacgacgata cggtctgcac cggactgctg gatgacgggc tcggccacgc ccaactcgtt
     7381 cacacggttg tgcagggtag tgatgttctg tttgaccgca tcggaacgca ctttattgac
     7441 cgcctcttcc gaaagcgtca agacgatatt gctgccgtct gaattcagcg ttgcttcagg
     7501 aaacagcttg cgcaactgcg gcagagcctt ttgcacatca cctgcatcct gcaaagggac
     7561 ggtcaggctg tttccagcct gacgcaccgt gccgctgcgg attttttcgc ggcgcagttc
     7621 gcggcggatg tcgcccgaat aacgttcaaa cgttttctgc atcgccgctt tcatatcgac
     7681 ctgcatggtg aaatgcacgc cgccgcgcag gtccaaaccc aaaaacatcg gattggcttt
     7741 gattttcgcc atccattcgg ggctgtccgc caacaggttg agcgcggtaa tatacccttc
     7801 gcccaaagtg ttttcgatga cgtcgcgcgc tttaagctgc gtttctgtgt ctttgaaacg
     7861 cactttcagt gaattgtcca caacaaacat cccgtcggtc tgaatacctg cgtttttcag
     7921 cgcggcatcc actttgaatt gagtctgttc gttgatgatg atggcttgtc ggttggtcga
     7981 tacctgcacg gcgggtgttt cgccgaatag gttgggcagc gaatacactg cggcaaccgc
     8041 aatcgtgaac acaatcagca gatatttcca taaaggataa cggttcatca ttgttcctta
     8101 atggttggaa ccccaccctt tcggtggtgt cggaatcggg ctatttcaga agaggcaaaa
     8161 acccttccca gccaggcaag accggaaagc ggcatcctga atatgccgcc ctgcgtgtcg
     8221 gaacatggtc aagccttcgg ttggaattca aaacaaagtg ccgcattcgg gctttccaga
     8281 tgcggcttgt cggcacaaat caatcgactt ttgcggcaat cgcattgcgt tccacttcga
     8341 cctcgatttt tgtaccctgt ccgatatcca cggtaaaaaa ctgttcgccg actctggtta
     8401 ccttaccctt gaaacctgcc gccaagacca ctttgtcgcc gactttcaag gcggcaagca
     8461 ttgcctgatg cgctttgaat ttcttttgct gcggacgcat gatcaggaag tagaacacca
     8521 ccataatcaa cactaaagga gcaaattgtg caacagcttg attcataatt tatccgttct
     8581 ttctaatatg gttgaaaatc gagaggggta tataataaca taagaccgta aacaatatat
     8641 cgggtttgcc gtccgtaccg accgtatacc gcagcctgcc cgtccacaaa cccatgtcct
     8701 tgacccgcct aatcttgaaa ttctatgcac tgttgcgcct ttttttgggc aaaaacgccc
     8761 gcaccgcatg gatttcgcat cccgcctgtg ccgggcacga acccggcgca aaccatcccg
     8821 attcgcccga ccgcatcctc tgcatcgagc aggcattgcg ccgcgccggt atttggcagc
     8881 acctccaaac catagaggcg gaagaaatca gcgatacgcg cctcgcactt gtccactcga
     8941 gcaaatatct gaaccgtttg gaatcttgcc tgccccaaaa aggcaagatt tcccgcctgg
     9001 ataacgacac tgcaatcagc acaggatcgc tgtctgccgc acgctttgcc gccggttcgg
     9061 cagttcaggc agtcgacatg gtcatgaacc gtaaagcatg gcatgccttt tgcgccgccc
     9121 gcccgcccgg acaccatgcg ggcagcggca aagccggcgg attctgcctg ctgaacaacg
     9181 ttgccgccgg cgtcatgcat gccattgccg aataccgcct gaaacgcatt gccgtcatcg
     9241 atttcgatgt ccactacggc gacggtacgg cagaaatatt caaagacgat ccgcgcatcc
     9301 tgtttttcaa cctgtttgaa accgaccttt tccccttccc cgaaaacaac gatatgcccg
     9361 acggcggcaa tatggtgcac ctgcccttgc cgccaggaac gggcagccgc acattccgcg
     9421 aagccgtccg caggcagtgg ctaccccgac ttgccgcatt caaacccgaa ctggtgctgc
     9481 tgtcggcagg attcgacgca caccgtctag acgaatcggg caggctcaac ctgcacgagg
     9541 cggattttgc ctggctgaca cacaaaatca ttcagacggc atcgggctgc cccggcaaaa
     9601 tcatatccgt actcgaaggc ggctacaccc ttgaacctct ggcacaatct gctgccgagc
     9661 atatccgcgt gctggcaggg ctgggcaaat ccgatgccgc aaccgcctat cagaaaacac
     9721 tggacccaac aaaaaaacgg tttgccaaac cgaaaaccgg acaggtgcgc caacctaccc
     9781 aatccgaccg ttac
//
LOCUS       DAWIVB010000061         9524 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWIVB010000061 DAWIVB010000000
VERSION     DAWIVB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.061"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..286
                     /locus_tag="WIX60_001067"
     CDS             <1..286
                     /locus_tag="WIX60_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ5305346.1"
                     /translation="HQVNSVDKETITLISKPKGNSPGGGRVVSSLPSKPPYHESNSIS
                     SLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLKNTRFKTHEVSLGMRYHF"
     gene            complement(260..469)
                     /locus_tag="WIX60_001068"
     CDS             complement(260..469)
                     /locus_tag="WIX60_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305347.1"
                     /translation="MPSERLSDGIFPCVLNRAHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGFMPSEGSDGIGIGESEVVAHAQ"
     gene            complement(487..1044)
                     /locus_tag="WIX60_001069"
     CDS             complement(487..1044)
                     /locus_tag="WIX60_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ5305348.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1120..2754)
                     /locus_tag="WIX60_001070"
     CDS             complement(1120..2754)
                     /locus_tag="WIX60_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ5305349.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3032..4138)
                     /gene="serC"
                     /locus_tag="WIX60_001071"
     CDS             complement(3032..4138)
                     /gene="serC"
                     /locus_tag="WIX60_001071"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ5305350.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4503..4934
                     /gene="rimP"
                     /locus_tag="WIX60_001072"
     CDS             4503..4934
                     /gene="rimP"
                     /locus_tag="WIX60_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ5305351.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4962..6464
                     /gene="nusA"
                     /locus_tag="WIX60_001073"
     CDS             4962..6464
                     /gene="nusA"
                     /locus_tag="WIX60_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ5305352.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6476..9364
                     /gene="infB"
                     /locus_tag="WIX60_001074"
     CDS             6476..9364
                     /gene="infB"
                     /locus_tag="WIX60_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ5305353.1"
                     /translation="MSNTTVEQFAAELKRPIEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNSSTISIRRTKTEVSTVDGVKVETRKRGRTVKIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSEKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAINDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
BASE COUNT         2545 a         2744 c         2405 g         1830 t
ORIGIN      
        1 acatcaagtt aattcggtcg acaaagaaac tattactctt atctctaaac caaagggaaa
       61 ctctccaggg ggaggccgtg tcgtatcaag cctgcccagc aaacctccct atcacgaaag
      121 caacagtatc agcagcttgg gtcttggtgt catcgccggt gtcggtttcg acatcacgcc
      181 caagctgact ttagacaccg gataccgcta ccacaactgg ggacgcttga aaaacacccg
      241 cttcaaaacc cacgaagtct cattgggcat gcgctaccac ttctgattcc ccgataccga
      301 tgccgtctga accttcagac ggcatgaaac ctttgcctgc gtacttggtg cgctggtcgc
      361 ctccgaacat ggcgcgacac ccgacatttc cgccgaacgc atcgggcgtt tcatgagccc
      421 ggtttaaaac gcatggaaaa atgccgtctg aaagcctttc agacggcatt ttgtttgaga
      481 ttccgtttac caatggctga caaacgcttc caaatcggta ttcttgggct tatgcacttc
      541 ctctgtcggc gtgccgacca tcatcagccc gatgatttta tccttatccg cacatccgaa
      601 agcctcacgc aacagggggc tgttgaccca catccccgtt atccagacat tgtcgaaacc
      661 ttgcgccgtt gccgccagtt gcagcgcata cgccgcacaa cccgccgtca gcatctgctc
      721 ccattccggt ttcggcttag gcacatcgcg gttcggcgca aacgttaccc cgataaccat
      781 cggcgccata ttgcccactt tttccgcctt tttcatcgca tcgtcgccga aattcaattc
      841 ggcaaccgtt tgctttaaca catcgcgaaa acgttgcaat cctacctcgc cttgaatcac
      901 ggtaaaacgg aaggggcgca tattgccgtg atcgggaact tgggttgccg cctgaaatat
      961 ttgttccaac tccgccgcat cgggggcggg gtgcttcagc tttttggaag atcggcggtt
     1021 cgtcaataat tttaaagcat ccatatcgtt atactccggt catcggtcgg gtgttcggac
     1081 acatatgccg tccgaaggct tcagacggca tatccggcat cagcgcggac ggcggcaggc
     1141 tgccaatata tccatttcct tccgataggt ttggctgttg gaaatgtcca tcagccccaa
     1201 taccgtgctg aaatagtggt cgtgcgaata ttcgttttcc gccgcttttt gtttgaggca
     1261 ttggaaatct atgcccccgt gttggcggaa ggctttggaa aaccacataa ccatcgggat
     1321 atgcgtctgc ccggaaggcg cgatggcgta aggcgcggcg tgcaggtaca tcccgttttc
     1381 gcccaaactt tcgccgtggt cggaaacata atgcaccacg ctttccaaat cgtcgcggtt
     1441 ttcaagtttg cggataacct tgtcgataaa ctggtccaca tacaaaaccg tattgtcgta
     1501 agtgttgacc agcgtggcgc gggtgcattt gttgatttcg ttggtgtcgc aggtaggcgt
     1561 gaatttgcgt tcggcttcgg tatagcgttc gtaatacgtc ggcccgtggc tgccgatggt
     1621 atgcaggatt aaaaccgcgt ctttatcgtt tttgttgagg acttcgtcga acttagtcag
     1681 caggatattg tcgaggcact cgccgttgcg gcagtattcg ggcaggttga gcgaggtaac
     1741 gtcggtattc ggcactttgc cgcacacgcc cttgcagccg gaatcgtttt ccaaccaagt
     1801 aacttccacg ccggcgcgct gcacgatgtc cagcaggttg tcttggtgtt cggctttgat
     1861 ttcgtcataa tccgtgcggt cgaaggttga gaacatacac ggcagggagt gcgcggtcga
     1921 tgtgccgcag cttctgacct gcgggaaatt gacaatttca tcgccgcgcg cggcaagcag
     1981 cggcgtagtt tggcggctgt aaccgttcaa accccagttg gcggcacgcg tggtctcgcc
     2041 cacgaccagc accacgaaac ggcgcaggct gccggccggc cggttttgca cgaccgccat
     2101 atccaattgc gtataaggaa tattggaacg cttccaatct ttgtatttcg acacgcccgc
     2161 gccgatgaaa ttagacggca caatcagatg ggttactgat ttattgttgc ggaaaaacga
     2221 ggcgtaatcc tgatattgca acattgcgat gcccaacgcg cacaaaaagg aaacggcggc
     2281 aagcacaagg cgtgtcaaaa gctccttata ccaaacgcgg tatttaacct tgacggcgat
     2341 atacgccagc gcgggcaata cgcccaaaca tacaatccac agcacatagc ccggcgtaat
     2401 caggcgcgcg ctttcggcag ccgtagtttg caagacatta ttcaacatcg acttgttgaa
     2461 atagatattg aaaaatattt cttggtaaga caccgccgca ctgataacca atatcaacgg
     2521 aatcaatacc ttatgcacga aaggcagggc aatgacgtga aaaacgaaat tacttaaaaa
     2581 aaacagcacc accggcatcg tatagaggaa gatatccgcc ccggtgccgt taaaaggatg
     2641 aagctcgaca actttggcaa aaaaggcgta attcaatacc agcgaggaat acagggaaag
     2701 gaaggcaatc agcgcggaag agccgagctt cggcctcagg ttcggtttta tcatttggaa
     2761 tgtgtcggat aagggttgga aaaggcatcc ggcatttgga atccggatta ttgaaaaaga
     2821 ttcttaatta taaggcaacg gagcaaagca gggcaagaaa acggaaggcg acgcaggctg
     2881 tcggatacac atctcggcac aaacagcatc acgcccggat tgaaaaggac ggatagccgc
     2941 cggcagcaca atcttaccac ctccaaaacg ccgccggaga acgccgaaac agccgatgcc
     3001 gtctgaagcc gcttcagaca acatcgggac atcaaccgta acgccgttgg aaatcgcgca
     3061 taaaatctgc caaagcccgc acgccttcaa gcggcatcgc attataaatg ctggcacgca
     3121 tcccaccgac agacttatag cccttcaaca ggcacaagcc ctgcaattcg gcttccagca
     3181 caaaacggcg gtcaagctcc tcatcccccg tttggaacac gacattcatt ttagaacgcg
     3241 cattcggacg gatacggttg atatagaaac cgccgctgcc atctatcgtc tcatacaagg
     3301 tttgcgcctt cagccgattg accgcttcaa tttttttcac accgccctgc gcctgtagcc
     3361 agcggaacac cagccccgac atataaatcg cgtaagttga cggcgtgttg tacataccgt
     3421 cgcggttcaa atgcgaacgg tagttgaaca catcgggaat atcgttcgga caacgctcga
     3481 gcaaatcctc acgcacaatc accaccgtaa ctcctgccgg cccgatgttt ttctgtgcgc
     3541 ctgcgtaaat cagtccgtag tcggcaacat caaactcgcg cgacaaaatc tcgctggaca
     3601 tatcgcacac cagcggcggc atgccttctg aaaggcgcgg cacttcacgg tattgcagcc
     3661 cgttgaccgt ttcattgacg gcaaaatgga caaacgccga atcgggtgca acatcccacg
     3721 tttccacagg cggcaggtcg agatagtcga actgctcgcc gccatgcgcc gccaaacgga
     3781 tttccgtatc ggtcaaacgg ctcatctgtt cataagcgat acggctccag ttgcccgtta
     3841 ccaccgcgtc ggcagtgcgg aaaccgtgtg ccagattcat ggctgccata ttaaattggg
     3901 ttgttgctcc gccctgcaga aacaatatct tatagttgtc aggcactttc aaaagctgcc
     3961 tcaaatcctg ttccgcatga tgcaggatgc tcaaaaacat ttccgaacgg tggctcattg
     4021 ccatcacagg aaaacccgta ccgttgtagt ccaacatttc ctgccgcgcc gtttccaaca
     4081 cggcttcggg caatacggca gggccggcgg aaaaattgta aatcggataa agagacatga
     4141 tgcagccttg attctgaaca ataacccgcc cgattttagg cttgcagcag gctttgtgca
     4201 aggatggaaa atacctgtcc tccgcccgat tccatgccgc ccgaacacgg aaaataatat
     4261 caatatattg atttacaaac ataaaaatca tgcacgcgac aaatagatac atttgttttg
     4321 tcaacaatat tcacgatttc ccattacaaa cctcccttac acccgctttt ttccgtccca
     4381 aaaacacaaa ataaatcaac actttcattt ctccgcaaaa gcggttataa tcacgccgat
     4441 ttttcaactt tgacgaaaaa tggccacggg gccatttttt tattatgtac atagggagca
     4501 gcatggatat ccaaaccatc ctcgaaaaaa ccctgcccgg cctgggctac gaactggtcg
     4561 atttcgaact gaccgcgcaa ggaacattgc gcgtgttcat cgacaaagaa agcggcatta
     4621 ccgtcgaaga ctgcgcaacc gtcagcaacc acttgagccg cgtcttcatg gttgaagaca
     4681 tcgactacaa aaacctggaa atttccagcc ccggactcga ccgccccttg aaaaaagccg
     4741 ccgacttcgt gcgctttgcc ggtcagaatg ccaaaatcaa aacccgcctg ccgatagacg
     4801 gtcagaaaaa ctttatcggt aaaatcgaag gctgcgaaaa cgataccgtt accgtatcct
     4861 tcgacggcaa aaccgtacaa atcgaattgg gcaacatcga caaagcccgt ctgcgccccg
     4921 aattcaaatt ctaaaacaca acaatattgg agatgttcaa aatgagtcgt gaaatgttac
     4981 agctggcaga agcactggca agcgaaaaaa acgttgatgc ggaagtcgtc ttccaagcac
     5041 tggaattcgc cctgtctacc gccgccaaga aaaaggcaga ccgcgaacac atggacgtgc
     5101 gcgtccaaat caaccgcgac accggcgaat accaaacctt ccgccgctgg ctgattgtcg
     5161 ccgatgaaga ctatacctat cccgatgtcg aaaaaaccat cgaggaaatc caagaggaaa
     5221 ttcccggcac taccatccaa atcggcgaat actacgaaga gcagctgccc aacgaaggct
     5281 tcggccgcca agccgcgcaa accgccaaac aaatcatcct gcaacgcatc cgcgatgccg
     5341 agcgcgagca gaatctgaac gagtttctcg ccgtcaaaga agacatcgtg tccggcacgg
     5401 tcaaacgcgt cgaacgccac ggcatcatcg tcgaagtcgt tgccggcaaa ctggacgcgc
     5461 tgattccgcg cgaccaaatg attccgcgcg aaaacttccg cagcggcgac cgcatccgcg
     5521 ccctcttcct gcgcgtcgaa gaaatcggca acaccggccg caaacaagtc attctgagcc
     5581 gtacttccgg cgatttcctc gtcaaactgt acgccaatga agtacctgaa attgcagacg
     5641 gcatgcttga aatccgcgct gtcgcccgcg acccgggaca acgtgccaaa gtcgccgtca
     5701 aagccaacga ccagcgcatc gatccgcaag gcacctgtat cggcgttcgc ggttcgcgtg
     5761 tcaatgccgt cagcaacgaa ttgtccggcg agcgcatcga tgtcgtcctc tggtcgcccg
     5821 aacccgcgca attcgtgatg agcgcgctct cacccgccga agtcagccgc atcgtcatcg
     5881 acgaagacaa acacgccgtc gatgtcatcg ttgccgaaga ccagctcgcg ctcgccatcg
     5941 ggcgcggcgg tcaaaacgtg cgccttgctt ccgacctgac cggctggcag ctcaacatca
     6001 tgacttccgc cgaggcagac gaacgcaatg cggcagaaga tgccgccatc cgccgcctgt
     6061 ttatggatca cttgaacgtg gacgaagaaa ccgccgacgt actggttcag gaaggttttg
     6121 caaccttgga agaagtcgcc tatgttcctg ccgccgaact gcttgccatt gaaggatttg
     6181 acgaagaaat cgtcgatatg ctccgcaacc gcgcccgcga tgccatcctg accatggcga
     6241 ttgccgccga agaaaaactg ggcgaagtgt ccgacgatat gcgcaacctc gaaggcatag
     6301 atgccgatat gctccgcagc cttgccgaag caggcattac cacccgcgac gacttggcag
     6361 agcttgctgt ggacgaactg attgaaatca ccggtgtaaa cgaagaaacc gcaaaagccg
     6421 tcatcctgac cgcacgcgaa cactggttta ccgaagacaa ataaaggggg tacagatgag
     6481 taacacaacc gtagaacaat ttgccgccga gctgaaacgc cccatcgaag acctgttgaa
     6541 acagttgaaa gaagccggcg tcagcaaaaa cagcggcagc gattccctga cgctggacga
     6601 caaacagctt ctgaacgcct acctgaccaa gaaaaacggc agcaacagca gcaccatcag
     6661 catccgccgc accaaaaccg aagtcagcac cgttgacggc gtaaaagtcg aaacacgcaa
     6721 acgcggacgc actgtcaaga ttccttctgc cgaagaattg gcagcacagg taaaagccgc
     6781 ccaaacccaa gccgcacctg tccggccgga gcagacggca gaagacgcgg caaaagcccg
     6841 agccgaagct gccgcacgcg cagaagcccg tgccaaggca gaagcggaag cggcaaaact
     6901 gaaagcggca aaagcaggca acaaagccaa acctgccgcg cagaaaccca ccgaagcaaa
     6961 agccgaaacc gcacccgttg cggcggaaac caaacccgcc gaagaaagca aagcggaaaa
     7021 agcccaagcc gacaaaatgc cgtctgaaaa acccgccgag cccaaagaaa aagccgccaa
     7081 gccgaaacac gagcgaaacg gcaaaggcaa agatgccaaa aaaccggcga aacctgccgc
     7141 acctgccgtg ccgcaacccg tggtcagcgc ggaagaacag gcgcaacgcg acgaagaagc
     7201 acgccgtgcc gccgcacttc gcgcccacca ggaagccctg ttgaaagaga aacaggaacg
     7261 ccaggcacgc cgcgaagcca tgaaacaaca ggcagaacaa caggcaaaag ccgcacagga
     7321 agccaaaacc ggcagacagc gtcccgccaa acctgccgaa aaaccgcagg cagccgcgcc
     7381 agccgtcgaa aataaacctg tcaatccggc aaaagcgaaa aaagaagacc gccgcaaccg
     7441 cgatgacgaa ggtcaaggcc gaaacgccaa aggcaaaggc ggaaaaggcg gacgcgaccg
     7501 caacaatgca cgcaatggcg acgacgagcg cgtacgcggc ggcaaaaaag gcaaaaaact
     7561 caaactcgag ccgaaccaac acgccttcca agcaccgacc gaacccgtcg ttcatgaagt
     7621 tttggttccc gaaaccatta ccgttgccga tttggcgcac aaaatggcgg tcaaaggcgt
     7681 ggaagtggtc aaagccctga tgaagatggg catgatggtt accatcaacc aatccatcga
     7741 ccaagacacc gccctgattg tggtggaaga actcggccac atcggcaaac ctgccgcagc
     7801 cgacgaccct gaagcattct tggacgaggg cgcggaagca gtggaagccg aagcattgcc
     7861 gcgtccgccc gtcgttaccg tgatgggcca cgtcgaccac ggcaaaacct cgctgctgga
     7921 ctacatccgc cgtaccaaag tggtacaggg cgaagcgggc ggcattacgc agcacatcgg
     7981 cgcgtaccac gttgaaaccc ctcgcggcgt gattaccttc ttggacaccc cgggccacga
     8041 agcctttacc gctatgcgcg cacgcggtgc gaaagcaacc gacatcgtga ttctcgtggt
     8101 cgccgccgac gacggcgtga tgccgcaaac catcgaagcg attgcccacg ccaaagctgc
     8161 gggtgtaccg atggtggttg ccgtcaacaa aatcgataaa gaagccgcca acccagagcg
     8221 tatccgccaa gagctgaccg cacacgaagt tgtgcctgac gaatggggcg gcgatgtaca
     8281 gtttatcgac gtttccgcta aaaaaggcct gaacatcgat gcattgctcg aagccgtctt
     8341 gctcgaagct gaagttttgg aactgaccgc acctgtcgat gcgcccgcca aaggcatcat
     8401 cgtcgaggcg cgcttggaca aaggccgcgg cgcggttgcc acattgctgg ttcaaagcgg
     8461 cacgctgaaa aaaggcgata tgctgctggc cggtacggca ttcggcaaaa tccgcgcgat
     8521 ggtcgatgaa aacggcaaat ccattaccga agccggtccg tccatccccg tcgaaatcct
     8581 cggcttgtcc gacgtaccga atgcgggtga agacgcgatg gtattggcgg acgagaaaaa
     8641 agcgcgcgaa atcgccctct tccgccaagg caaataccgc gacgtgcgcc ttgccaaaca
     8701 gcaggcggcg aagctggaaa atatgttcaa caatatgggc gaaacccagg cccaatcttt
     8761 gtcggtcatc atcaaggcag acgtgcaggg ctcttacgag gctttggcgg gcagcctgaa
     8821 aaaactgtcc accgacgagg tgaaagtgaa cgtgttgcac agcggcgtag gcggcattac
     8881 cgaatcggat gtcaaccttg ccatcgcttc gggcgcattc attatcggct ttaacgtgcg
     8941 tgcagatgcc tcttcgcgca aacttgccga aaatgaaaac gtggaaatcc gctactacaa
     9001 catcatctac gatgccatca acgacgtgaa ggcggcgatg agcggtatgc tttccccgga
     9061 agagaaagaa caggttaccg gtacggtcga aatccgtcag gtcatctccg tttccaaagt
     9121 cggcaacatt gcaggctgta tggttaccga cggcgtggtc aaacgcgatt cccatgtccg
     9181 cctcatccgc aacaacgtgg ttatccacac gggcgaactg gcttcgttga aacgctataa
     9241 agacgatgta aaagaagtcc gcatgggctt cgagtgcggt ctgatgctca aaggctacaa
     9301 cgaaatcatg gaaggcgacc aactggaatg cttcgacatc gtcgaagttg cccgcagcct
     9361 gtaattcctt tgcaaataaa atgccgtctg aagcgttcag acggcatacg aaacgggttc
     9421 tgtatcatac agaacccgtt ttttgtcgca aatcggcttc agacagccct cttgccttat
     9481 cccgatttga atctgacttg ccatacaaac aggcttcaga cggc
//
LOCUS       DAWIVB010000062         9470 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWIVB010000062 DAWIVB010000000
VERSION     DAWIVB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.062"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            89..298
                     /locus_tag="WIX60_001075"
     CDS             89..298
                     /locus_tag="WIX60_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ5305354.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            387..1601
                     /locus_tag="WIX60_001076"
     CDS             387..1601
                     /locus_tag="WIX60_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ5305355.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WIX60_001077"
     CDS             complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WIX60_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ5305356.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRS
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLAV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4528..5538
                     /gene="trpS"
                     /locus_tag="WIX60_001078"
     CDS             4528..5538
                     /gene="trpS"
                     /locus_tag="WIX60_001078"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ5305357.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNLDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDTPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAAKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5607..6152
                     /locus_tag="WIX60_001079"
     CDS             5607..6152
                     /locus_tag="WIX60_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305358.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENTAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLDLGQSSEGKTNDGKKQISYPIKGLPEQNVIRLIGKHPGDLEAVSGKCME
                     TDDKDSPAGWAENGVCHTLFAKLVGNIAEDGGKLTDYLVSHAALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            6472..6795
                     /locus_tag="WIX60_001080"
     CDS             6472..6795
                     /locus_tag="WIX60_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305359.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(7004..8512)
                     /gene="ppx"
                     /locus_tag="WIX60_001081"
     CDS             complement(7004..8512)
                     /gene="ppx"
                     /locus_tag="WIX60_001081"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ5305360.1"
                     /translation="MTTTPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8629..9360)
                     /locus_tag="WIX60_001082"
     CDS             complement(8629..9360)
                     /locus_tag="WIX60_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ5305361.1"
                     /translation="MSREINAGRHHFLYGGISGGGVHYWDNKDFSEQSLRLSFGYKNR
                     SVTRSFGIVPFVEQNLLGGSRYNFVGGFNADFSQRLSERWRLTLNAGNMWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHNITKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRMFDAPGTIVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNMQIFMSVEKDFK"
BASE COUNT         2201 a         2443 c         2500 g         2326 t
ORIGIN      
        1 gatttgaatt tttcagaaaa tgaagcggac gtttgggcgg aggcaacttg tttgataaga
       61 tagcaatatt ttaaaacgga gaaagatcat gccttatgtc aatattaaag taaccggcgg
      121 caaggaagca ccgactgccg cgcaaaaagc ggaactgatc ggcggcgtaa ccgaattgct
      181 ggcacgcgtg ctgggcaaaa atcccgaaac aacggttgtc gtgattgacg aagtggatac
      241 cgataactgg ggaataggcg gcaaaagcgt cagcgaacgg cgcaaagagg gcaggtaaaa
      301 agcctgaaaa tctcggtttg atgctttaaa ttccgcgtga aaaagagtac attcccaccc
      361 attgcccaaa atttacggaa cacatcatgg ataaatttcc caagtctgca aagctcgatc
      421 acgtctgtta cgacatacgc gggccggttc acaaaaaagc ccttcagttg gaagaggagg
      481 gcaataaaat ccttaaactc aatatcggca accctgcgcc gttcggcttt gaagcccctg
      541 atgaaatctt ggtcgatgtc atccgcaacc tgccgacttc gcaaggctat tgcgattcta
      601 aagggctgta ttccgcccgc aaagccattg ttcactacta tcagaccaaa ggtttgcgcg
      661 atattacggt tgatgatgtc tatatcggca acggcgtgtc cgagctgatt acgatgtcta
      721 tgcaggcatt gctcaacgac ggcgacgaaa tcctgattcc cgcgcccgac tatcccttgt
      781 ggacggcggc ggcaacgctt gcgggcggta cggtacgcca ttatctgtgc gacgaagaaa
      841 acggctggtt ccccaacctt gccgatatgg aagccaaaat cacgcccaaa accaaagcca
      901 tcgtcgtcat caatcccaat aatccgacag gcgcggtgta cagcagggaa atcctgttgg
      961 aaatcgccga actggcgcgc aagcacggtt tgattatttt cgccgacgag atttacgaca
     1021 aaatccttta tgacggcgcg gttcaccacc acatcgccgc gcttgccccc gaccttttga
     1081 cggtaacgtt caacggtttg tccaaagcct accgtgtagc cggattccgc caaggctgga
     1141 tggtgctcaa cgggccgaaa catcatgcaa aaggttacat cgagggtttg gatatgctct
     1201 cgtctatgcg cctgtgtgcc aatacgccga tgcagcacgc gattcagacg gcattgggcg
     1261 gctatcagag catcaacgaa ttcattttgc ccggcggacg gcttttggaa cagcgcaaca
     1321 gggcgtggga actggtcaac cagattcccg gcgtatcctg cgtcaaaccg atgggcgcga
     1381 tgtatatgtt cccaaaaatc gataccgaaa tgtaccgtat ccgcgatgac atgaaattcg
     1441 tttacgattt gctggtgcgc gaaaaagtct tgctggtgca gggaacgggg tttaattgga
     1501 tcaagcccga ccatttccgc attgttacgc tgccttacgt ccatcagatt gaagaggcga
     1561 tgggcaggct ggcaagattc ctgcaaacct accgccaata aggggacggt ttgtctgccg
     1621 aggataaaaa atgccgtctg aaacggagat tcccgtttca gacggcattt tcaacagcag
     1681 gaacgaatca ggcaaatttc agtctgtcgc cgtcggcttc caccctgatt tcgctttcgg
     1741 gcgcatagtt tccggcaagc agggcttttg ccagcgggtt ttcgatttcc gactggatgg
     1801 cgcgtttgag cggacgtgcg ccgtaaatcg ggtcgaaacc ggctttggcg atgatgtcca
     1861 gtgcggcatc ggaaacagcc aggcgcaggt tttgtttttc caaacgtttt tccaagcctt
     1921 tgagctggat tttcgcaatg ttgcggatat tatcctgatc cagtccgtgg aacacgacca
     1981 cttcgtcgat gcggttgatc atttcggggc ggaaatgttc tttcacatcc tccatcacaa
     2041 cttctttcac cgcttcgtaa tcctgaatgc ccatttgttg gatatgttgg ctaccaatat
     2101 tggaagtcat cacgataacg gtatttttga agtccacggt gcgaccttgt ccgtcggtca
     2161 agcggccgtc atccaatact tgcagcagga tgttgaacac atcgggatgg gctttttcca
     2221 cttcgtccag cagaatcacg ctgtacggtt tgcggcgcac ttgttcggtc aggtagccgc
     2281 cttcttcgta gccgacatag cccggaggcg cgccgattaa gcgggcaacg gcgtgttttt
     2341 ccatatattc ggacatatcg atgcgaatca gatgatcttc gctgtcgaac agaaagcctg
     2401 ccagggcttt acacaactcg gttttaccca cgccggtcgg gcccaagaac aggaagctgc
     2461 cgtaaggctt gttcggatcg gcaagaccgg agcggctgcg gcggatagcg tcggacacgg
     2521 cacgcacggc ttcgtcctgt ccgaccacgc ggcggtgcaa tacttcttcc attttcagca
     2581 gtttgtcgcg ttcgccttcc atcattttgg atacgggaat gccggtcata cgggaaacca
     2641 cctctgcgat ttcctctgcg ccgacattat tacgcaagag tttgtttgcc ggttttgtgc
     2701 tgtccgtatc tgcccgttcg gcggctgcac gctgtttttc caaatgctcc aaatcttcat
     2761 acatcaattt tgaagccagt gccaaatcgc cttgccgttt tgcctgttcg attttaattt
     2821 tgacttcgtc aatttgtttc ttaatattag cagcaccgtc tgaaattgct ttttcggctt
     2881 tccagatttc gtctaaatcg gcgtattctt tttgcagacc gttgatttcc tcgtctatca
     2941 gttccaaacg ttttttgctg gcatcgtctt tttctttttc aacgtgcgcc ttttccatcc
     3001 gaagctgaat tagacggcgg tcgattttgt ccattgcttc cggcttggtt tctttttcca
     3061 tcttgacacg gctggcggct tcgtcaatca aatcaatcgc tttatcgggc aggaagcggt
     3121 cggtaatgta gcggtcgctc aactccgctg cggcaacgat agcagggtcg gtaatatcga
     3181 taccatggtg gatttcataa cgctcctgta aaccgcgcaa aatagcgatg gtgtcttcca
     3241 cgcttggctc gccaaccaat actttttgga agcggcgttc gagtgccgca tctttttcga
     3301 tgtattggcg gtattcgtcc aaagtggtcg cgccgataca gtgcaattcg ccacgtgcca
     3361 aagccggttt cagcatattg cccgcgtcca tcgcgccgtc ggttttgccc gcgccgacca
     3421 aagtatggat ttcatcaatg aaaatcagag tgttgccgtc gtctttcgcc aaatcgttca
     3481 acacgccttt caagcgttct tcaaattcgc cgcggtattt cgcgccggca atcaaagccg
     3541 ccaaatccaa aaccagcaag cgtttgttac gcagggattc aggtacttcg ccgttgacga
     3601 tacgttgcgc caagccttca acaatggcgg ttttacccac acccggctca ccaatcagca
     3661 cagggttgtt tttggtacgg cgttgcaata cctgaatcgc gcggcggatt tcgtcgtcac
     3721 gaccgataac ggggtcaagt ttgccgtcgc gggcgcgctg ggtcaggtca agcgtatatt
     3781 ttttcaaagc atcgcgttgg tcttcggcat tggcatcgtt cacgttttgt cctcctcgta
     3841 ctgcgtcaat cgcggcattg atgttttgtt cggtcgcgcc ggcttctttc aaaattttgc
     3901 cggtcgcatc gttctgctgt accaaggcaa gcaggaaaag ttcgctggca atataggcat
     3961 cgctgcgttt ggtggcagct ttgtccatca ggttcaacac cgcctgcaat tctcggctgg
     4021 gcagaatatc gccgccctga ccggacactt tcggcaggct gtttaaatgc tgctgcaaac
     4081 gctgtttcac ctgcggcacg ttcacgcccg catgagccaa gagcgcggcg gctccgctgt
     4141 tttggtcgtc aagcagggct tttaacacaa agcccgcttc cagatagctg ccgtcagcag
     4201 ccaacgccaa actctgagct tctgcaaggg cttgttggaa tttggcggtt aatttgtcgt
     4261 atcgcatttt tgtttccttt tcaaaatgtc cgctgtcgaa gcctatatgt gcataattgt
     4321 ggataactca agttctgttt tctgtttttc tatatttaat tcgatatatc attgaattta
     4381 aagtatataa aaatgtataa taatgtgtat aactatatct tcttaatatg gaaaagtctg
     4441 ttgtcggctg gatgtaggtg gcaaatcggg tataatcggc acatcttttt ccctttcaga
     4501 cggcattgat gccgcaagga catttttatg agcaaaaaac gagttctgac cggcgtaacc
     4561 accaccggca tcccgcatct gggcaactac gtcggcgcca tccgccccgc cgtccgcgcg
     4621 gcgcaaaacc tcgataccga atccttcctc ttcctcgccg attaccacgg tatcatcaaa
     4681 tgccacgagc cggagatgat tcaccaatcc acccaagccg ttgccgccac ttggcttgcc
     4741 tgcggactcg accccgagcg caccaccttc taccgccaaa gcgacactcc cgaagtgatg
     4801 gaattgaact ggattctgac ctgcatcact gccaagggtt tgatgaaccg cgcccatgcc
     4861 tacaaagccg ccgtgcaggc aaatgcagaa aacgggcagg aagaccctga tttcggtgtg
     4921 gaaatgggtt tgttcagtta tccgattctg atgactgccg atattctgat gttcaacgcc
     4981 aacgaagtgc ccgtcgggcg cgaccaaatc caacacgtcg aaatggcgcg cgacatcgcc
     5041 ggccgcttca accaccgctt ccgggaactc ttcaccctgc ccgaagtgaa aatcgatgaa
     5101 aacgtcgaac tcttggtcgg tttggacgga cgcaaaatgt ccaaatccta cggcaacacc
     5161 attccgcttt gggaaaacga caaaaaaacc caaaaatcgg tcaacaaaat catcaccaat
     5221 atgaaagagc cgggcgagcc gaaacagccc gacgaaagcc cattgtttga aatctacaaa
     5281 gccttctcca cgccgtctga aacggtggaa tttacgaaaa tgcttgccga cggcttggcg
     5341 tggggtgaag ccaaaaaact tttggcggcg aaaatcaacg ccgaactcgc cgaaccgcgc
     5401 gaacgctaca acgagctgac cgccgaccct tcgcaaatcg aagagatttt gcaggcaggc
     5461 gcggcgaaag cgcgtaaaga agcacgcgaa ttattggaca aagtacgcga tgcggtcggc
     5521 atccgcccgt tgaaatgaac ccgatgccgt ccgaacctcc gcctgccgcg tttcagacgg
     5581 cattttgaaa ccatcaggag tgtggaatgc tgaaaacatc ttttgccgta ttgggcggct
     5641 gcctgctgct tgccgcctgc ggcaaatccg aaaatacggc ggaacagccg caaaacgcgg
     5701 tacaaagcgc gccgaaaccg gttttcaaag tcaaatatat cgacaatacg gcgattgccg
     5761 gtttggattt gggacaaagc agcgaaggca aaaccaacga cggcaaaaaa caaatcagtt
     5821 atccgattaa aggcttgccg gaacaaaatg ttatccgact gatcggcaag catcccggcg
     5881 acttggaagc cgtcagcggc aaatgtatgg aaaccgatga taaggacagt ccggcaggtt
     5941 gggcagaaaa cggcgtgtgc cataccttgt ttgccaaact ggtgggcaat atcgccgaag
     6001 acggcggcaa actgacggat tacctagttt cgcatgccgc cctgcaaccc tatcaggcag
     6061 gcaaaagcgg ctatgccgcc gtgcagaacg gacgctatgt gctggaaatc gacagcgaag
     6121 gggcgtttta tttccgccgc cgccattatt gaggtattcg gacatcccgg aatatatttg
     6181 ggtttttcaa accctgcagg aaaaagtccg caccgtcggg aaactcaaaa ggagggggat
     6241 atgtgttaca attttccgaa ctgttttcat aaaatagttt tcggacgtgt ttcaatatgg
     6301 cattgatgcc gccttattgt tcgagaaaaa aacctttata tttaaatata atgggtttta
     6361 actaaacggg aaaccgtttt ctctccggtc gatgggcaaa atcagccgat tgatggaaca
     6421 cggtccgatt tttaagcaaa accttaatca acaactcaag aaggataaga catgaaaaaa
     6481 ttattgattg ccgcaatgat ggcggctgcc ttggcagctt gttcgcaaga agccaaacag
     6541 gaggttaagg aagcggttca agccgttgag tccgatgtta aagacactgc ggcttctgcc
     6601 gccgagtctg ccgcttctgc cgtcgaagaa gcgaaagacc aagtcaaaga tgctgcggct
     6661 gatgcaaagg caagtgccga ggaagctgta actgaagcca aagaagctgt aactgaagca
     6721 gctaaagata ctttgaacaa agctgccgac gcgactcagg aagcggcaga caaaatgaaa
     6781 gatgccgcca aataatttgt tgccttggca aaaatgatgg gatgccgcct gccggcaacc
     6841 caaacgaaac cgcctgaaga ttttcaggcg gtttttgggt tatgtgcccc ttgtttttta
     6901 cgcctgcatg accgttccga gcagatgtgc cgcgcatatc gggatgttgc ggacaggcaa
     6961 atgccgtctg gacagggttt ggacggcatt tgttccgcaa ggttcagacg gcctcgactt
     7021 tgaacggcat attgattttt tgccattgga cgctttcata ttccaatgcg tcggcaatca
     7081 ggggatggcg ttccagccat tccctgtcaa tacgcaggat gaagccgcag ctttccgtat
     7141 ccgtgcgcaa ctgcatattt ttcgggaaag acaggtcttg gcgcgaacgg cagaacagtg
     7201 cggcaaggcg cagggacaaa acggcatacc acaacatttc gttggtgccg atgatgccgc
     7261 tcattttttt catatcgccg cgatgaccga tgaccagttg ggcaagtatg gtctgttctt
     7321 tgcgtgagaa acccggcata tcggcgtttt cgaggatgta ggcggaatgc ttgtgatagc
     7381 cggtgtgggc gatgtccaaa ccgatttcgt gcagcgcggc ggcgcgtccg agatactgtt
     7441 gccacaaggc aagctcttga actgtaacgt ttttagcgtg gcagaggctg tccataaagg
     7501 tttgcgcggt ctcggcggtg cgtttcgcct gattgaggct gacgtggtag cggtgttgga
     7561 actcggcaac cgtttgtccg cgcatatctt cgtttaaacc gcgcccgatc aaatcgtaaa
     7621 acacgccgtc gcgcagggcg gcttcggtta cggtcatcct gtcgagtttc atttcctcaa
     7681 acgccgccat catcacggca agtccgccgg caaaaacttc gatgcgttcc ggtttcaggt
     7741 tttcaaattt ggcttttttg accgaaccgg cttcgatgat gcgttcggcg agggcgcgca
     7801 tgcctttgta ggtaatgtcc gcctcttggg gcatttcggc ggcaagcacg tcgcggatgg
     7861 attttgccga acccgatgtg ccgacggcga aatcccaacc ttcgcgcctc atatttttgc
     7921 tgatacgctg gatttcgttg cgggcggcgg aaatggcaga ttggaagtct ttggcggtga
     7981 ttttgttttg gaagaagcgc aggctgtagg ttacgcagcc caagggcagg ctttcggtaa
     8041 tgtcgggatt cagcgtcgag ccgatgacaa attctgtcga accgccgccg atgtcgataa
     8101 ccagcatttt gccgccgccc ggggggaggg tgtggatcac gccggtataa atcagccgcg
     8161 cctcttcgcg cccggcgatg atttcgatgg ggaaacccaa tgccgcttcg gctttgggaa
     8221 ggaaatctgc gatgtttttg gcaacgcgga atgtgttggt tgccacggcg cgtacctgtt
     8281 cagggcggaa gccgcgcagg cgttcgccga attttgccag acagtccaaa gcctgttctt
     8341 gggaagcggc actcagattt ttctgttcgt ccagtccggc ggcgaagcgc accatctgtt
     8401 tgaacgaatc gatgactttt aattgtccgt tgttgttttc gcaaatctgg aggcggaaac
     8461 tgttggaacc caaatcgacg gaggcgagga cgtttgcggg ggtggtggtc atggcggata
     8521 ccggtggggg aaaaacgcaa tgttactctg acggcgcagg cgttgacaat aaatgatgcg
     8581 gcggtttttg attctgccca cggatgttgc cgacggcatt ttttgcgctt atttgaaatc
     8641 cttttccacg ctcatgaaaa tctgcatgtt tttgcgtgtg taaaaacttt tcatattgct
     8701 gtcgattttc agatagcgga aattgagttg cggcgtaaag cccttccaag agattttgtc
     8761 atgccacaac gacaggtttg cctgatattc gtggtctttg cgcgggaagc ggtacacaat
     8821 ggtcccgggt gcgtcaaaca tcctgcgggt atagcgcagg tttgcccgca gacccaagcc
     8881 gccgtcgaac gttttgaccg cgccgacacg caaacccttg cggatggaag cctgttccgc
     8941 ctctttcgtt atgttgtgcg accagtccgc accgccgtaa agcagccagt ctttcggcgc
     9001 ggaatacatc agcgtcgcgc ccgccagcgg catatggctg tcgtatcggg cggcggtgcg
     9061 gtcttcctga taatgcttcc acatattgcc cgcgtttagt gtcaaccgcc agcgttcgct
     9121 caagcgttgg gagaaatcgg cattgaagcc gccgacgaaa ttgtatcggc tgccgcctaa
     9181 gaggttttgc tcgacaaacg gcacgatgcc gaacgagcgc gttaccgaac ggtttttata
     9241 gccgaacgac aggcgcaggc tctgttcgct gaaatctttg ttatcccaat aatgcacgcc
     9301 gccgccgctg atgccgccgt agaggaaatg atgcctgccc gcattgattt cgcgcgacac
     9361 gcctaagccc tagcgcaaac cgtgtgcctt ttgcggcagg ctgtcggcgg ttttcgtcca
     9421 gcttctgccc gcaaattcaa tcgttttttc ggacgaagcg ttgtttatag
//
LOCUS       DAWIVB010000063         9433 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWIVB010000063 DAWIVB010000000
VERSION     DAWIVB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.063"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(38..763)
                     /locus_tag="WIX60_001083"
     CDS             complement(38..763)
                     /locus_tag="WIX60_001083"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ5305362.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDTGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDESGETRLAGGFYGVQIGSV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="WIX60_001084"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="WIX60_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ5305363.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1486..1863
                     /locus_tag="WIX60_001085"
     CDS             1486..1863
                     /locus_tag="WIX60_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ5305364.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1873..2682
                     /gene="dapB"
                     /locus_tag="WIX60_001086"
     CDS             1873..2682
                     /gene="dapB"
                     /locus_tag="WIX60_001086"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ5305365.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAGEKTGVVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEAHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2679..2924)
                     /locus_tag="WIX60_001087"
     CDS             complement(2679..2924)
                     /locus_tag="WIX60_001087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305366.1"
                     /translation="MPRKQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPFQIRTTH
                     RATLYVSSRVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(2942..3337)
                     /locus_tag="WIX60_001088"
     CDS             complement(2942..3337)
                     /locus_tag="WIX60_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305367.1"
                     /translation="MPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFPQEIPT
                     PNYTDWVKIKFKQFSYLKFIYGYATKSQDINIDNTLELGELKQDDEILDYGGALEVIG
                     GRYDLLTAFRIDIVCREIELEFLDQEGFN"
     gene            complement(3355..3600)
                     /locus_tag="WIX60_001089"
     CDS             complement(3355..3600)
                     /locus_tag="WIX60_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305368.1"
                     /translation="MDYLLEAARITKEGGEIVINGTSNNKYLRGIPNEAELARMGLRL
                     KYNGLLKEEFKQLKFHLNNGGDLRSSNYKTIVFEKVK"
     gene            complement(3608..4780)
                     /gene="lpxB"
                     /locus_tag="WIX60_001090"
     CDS             complement(3608..4780)
                     /gene="lpxB"
                     /locus_tag="WIX60_001090"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ5305369.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAERLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRKTARKTLGVDADIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRVLHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4828..5820)
                     /locus_tag="WIX60_001091"
     CDS             complement(4828..5820)
                     /locus_tag="WIX60_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ5305370.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAERKMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            6314..9073
                     /locus_tag="WIX60_001092"
     CDS             6314..9073
                     /locus_tag="WIX60_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ5305371.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         2358 a         3032 c         2225 g         1818 t
ORIGIN      
        1 ccaccatatt tgaacctgac cggagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatcagcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgtg tcctgctggc
      181 agtctatcag ttccacgccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgcaac gcaaacatcg attcgccata aaacacgctg ccgatctgca
      301 cgccgtaaaa gccgcccgcc aacctcgttt caccgctttc atcgggataa tggcactcga
      361 aagaatgcgc gtaccccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagttcc gtcctgattc gggcgcgccg ctgccgcaca atgcgcgacc acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttgcgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaatcaccg cacgcggacc gaccgcatac caaaaaaacc
      601 aaccgtcccg agaaaaccac ggaaacacgc cgttccgata cgcctcaagc agccgccccg
      661 tatccaaatc gccgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcttccgcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcgtg gtggtcgccc ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatggt ggcgttgcag aatacccgcc tgctcaaact
     1081 gggtcagcac acggtaaatc gtcgccacac cgatttccac accctcttcc aacaaaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcatgcgt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgtgcaata ttgttgaatt
     1261 tttccataat attcaatacc cctgtaaaac aatagacgtt ataatacgca atttcggcct
     1321 gtttgcccac tatcgcacca tagcagtttg caatagtaaa accgcaccgg cggccgatgc
     1381 gcggacaggg cggataccgc ttaatcgtat gattatcgtt cgcttttata aaatattcaa
     1441 gcagtgttac actacccatc ccgatttgca cagaaaggca tttccgtgaa caaaaccctc
     1501 atcctcgccc tttccgccct cctcggcctt gccgcgtgca gtgccgaacg cgtttcactg
     1561 ttcccctcgt acaaactcaa aatcatacag ggcaacgaac tcgaaccgcg cgccgttgcc
     1621 gccctccgcc ccggcatgac caaagaccaa gtcctgctcc tgctcggcag ccccatactg
     1681 cgcgacgcat tccataccga ccgctgggac tataccttca acacctcccg caacggcatc
     1741 atcaaagaac gcagcaatct gaccgtctat tttgaaaacg gcgtactcgt ccgcaccgaa
     1801 ggcgacgtcc tgcaaaacgc tgccgaagcc ctcaaagacc gccaaaacac agacaaacca
     1861 taaggaacac acatgacacc gctcaaaatc gccatcgccg gcgcaaacgg ccgtatggga
     1921 cgcgtattgg ttgaagccgt caacaaccat cccgacaccg tcctttccgg cgcgctcgaa
     1981 cactcgggct cagaagccct cgggctggac gcaggctacg ccgtcggact caaaaccggc
     2041 atcgccattt cagacgacgt tgacgccgtt ctcgcacaaa gcgacgtact catcgacttc
     2101 acccgccccg agcccaccct caaacacctg caaaaatgcg ttgaaaaaca agtcaacatc
     2161 atcatcggca caacaggctt cgacgatgcg ggcaaagccg ccatccgggc cgccggcgaa
     2221 aaaaccggcg ttgtcttcgc cgccaacttc agcgtcggcg tgaacctcac cttccacatc
     2281 ctcgacaccg tcgcacgcgt attaaacgaa ggctacgaca tcgaaatcat cgaagcccac
     2341 caccgccaca aagtcgatgc ccccagcggc accgccctgc gtatgggcga agtcatcgcc
     2401 ggcgcgctcg gccgcgacct caaacaatgc gccgtttacg gccgcgaagg ccacaccggc
     2461 ccgcgcgatc cttccaccat cggttttgcc accgtccgcg caggcgacat cgtcggcgac
     2521 cacaccgccc tcttcgccac cgacggcgaa cgcgtggaaa tcacccacaa ggcaagcagc
     2581 cgcatgacct ttgccgccgg tgccgtccgc gccgcagttt gggtaaacgg caaaacgggt
     2641 ttgtacgata tgcaggacgt actcgggctg aacaaccgtt aacccccata caaaatgccg
     2701 tctgaaaaga tattgttcag acggcatttt gccgacaggc tccgtatcgg catatgtttc
     2761 aacacacggg acgatacata aagcgtcgcc ctatgtgtcg tcctgatttg gaagggatta
     2821 cgcccctccc aaataaagtc tgattctacc gccccgaagg acagatgtcc gagtggcggg
     2881 gtttcaaccg aaaaggaaat acgataaagt tgcttgcgcg gcatgacaag ctacggctcg
     2941 tttaattgaa accctcctga tctaaaaatt ctaactctat ttcccggcaa actatatcta
     3001 tcctaaaagc agtcagaaga tcatacctac cgcctatcac ttccagcgca cctccataat
     3061 ccaagatttc atcatcctgc tttaattcgc caagttccaa tgtattatcg atatttatat
     3121 cttggctctt tgtagcgtat ccatagataa atttcagata gctgaactgc ttgaatttaa
     3181 tttttaccca gtctgtataa tttggtgtag gaatctcttg aggaaagtcc ggataatcca
     3241 cattctggac gatgatatgg aattcatcgt ttttcaaagt atagtttaat agagtgccct
     3301 catatatata cagaaattga taatcttttt ttggaatagt attaagttgt catattattt
     3361 taccttttca aaaacaatgg ttttataatt acttgatctc aagtcgcctc cattgttcag
     3421 atgaaatttc agttgtttaa actcttcctt taacagacca ttatatttta accttaatcc
     3481 cattcgggcc agttctgctt catttggaat tcctcttaaa tacttattgt ttgatgtgcc
     3541 attaatcaca atttctcccc cttcttttgt aatacgtgct gcttcaagaa gataatccat
     3601 taaccgctca acatcccgcc tcttccaaaa ccgcgcgcgc ggccaaatcc gccgtatctt
     3661 ttttcaacag caggtgcaac accctgaaat cctgttgcag cgcggcaacc ttatcggggt
     3721 gttcgtacca gtccgccaac gccgccgcca gtttttccgg tacggcatca tgttgaagca
     3781 gctccggcac agcctcctta cccaacagga tattcggcag gccaacatgc ggcactttga
     3841 ttttgcgttt cacataagca taagtcagcg gcgaaatctt gtagctgatg accatcggac
     3901 gcttgcacag cgccacctcc aaagttgccg taccgctcgt taccagcacc gcatccgccg
     3961 ccctgcacac cgtttcagac tgtctgtcgg ttaccgtcag cggcaggccg gcaaactccg
     4021 gccgctgcaa aacttccgcc aaacgccgct tcgtcgcctc cgttgcggca ggcagcagga
     4081 agcatgcggc aggatagcgt tccaacaaca ataatgccgt ctgaaaaaac accggcgcca
     4141 tataatcgat ttcgctgacg cggctgccgg gcagcagggc gaatacgggg atgtcggcat
     4201 ccacgcccaa agttttccgc gccgttttac ggtcgtcttc caagggcata agctgcgcca
     4261 tcggatgacc gacaaactcc gcacgtccgc ccgcatcgag ataaagctgc ggctccatcg
     4321 ggaacaggca cagcacgcgg ttgacctgat gcacgatttt gcccacgcgt tcccgccgcc
     4381 acgcccacac cgacgggctg acataatgca cggtcggaat ccccgaccgt ttgagccgct
     4441 ccgccacacc caaattaaaa tcgggcgcat cgataccgac aaagacatca ggtttcaacg
     4501 acagcaaatc ccgtaccagc cccctgcgta tccgtaaaat ttccggcagc cgcctgacca
     4561 cttcgacaaa gccgcgcacc gccagccgct cctgatcata aaggctctcg aaaccttccg
     4621 ccttcatcag ttcgccgccg ataccgacaa aacgcgcctg cggacaacgc ttgcggatgg
     4681 cgcgtatcag gtgcgccccc aacaggtcgc ccgacgcttc gccgacactg acagcaatca
     4741 aagggctttt tttatcaacc atattcgtct gtccccacat actttcacgt cccgatgccg
     4801 tctgaaggct tcagacggca tcggggttca cattttcgtc ccgttttcca acatcaccgc
     4861 aaactgtgcc aaatccggcg gcagctccgc cttcaacacc aacggctcgc ccgtgagcgg
     4921 atggttcagg tgcagctcgg acgcgtgcaa aaacatccgc ttcaaaccca acttctgcaa
     4981 acgccggttc gcctgataat cgccgtagcg ttcgtcgccc gcaatcggac agccttgaga
     5041 ttgcaggtgg acgcggattt ggtgcgtgcg ccccgttttc aacgtcgccc gcaccaaagt
     5101 caggtgcgac agcccgacac cgtgcaaaat gccgtctgaa aaacggctta acacacggaa
     5161 caccgtatgc gccgactgcc cgtccgcact gacgcgcacc atcttttcgc cttgtgcgcc
     5221 ggtatatttg aacaggggca gtttgacatg gaaattgtcg tccggcagtt tgcccacccc
     5281 cagcgcaagg tagatttttt tggggtggtc gttacggatg gcttcgtgaa gtttgacgag
     5341 cgcgctgcgt ttcttcgcca ccatcaacaa gccgctcgta tccttgtcca aacgatgaac
     5401 caactccaaa tacttcgcct ccggacgggc gcggcgcaac tgttcgataa cgccgaaact
     5461 cacgccgctg ccgccgtgga cggcaacgcc ggacggtttg tcgatgacca aaagcgcatc
     5521 gtcttcgtaa acaacgtcaa acgcacgcgc cggtacggcg gcacgccttt cagacggcat
     5581 cttgcgctcc gccacgcgca caggcggaat ccgcaccgta tccccctccg caatacggct
     5641 gtcgggtttg cagcgtttct tgttcaaccg cacctcgccg gcgcggataa tgcggtggat
     5701 atggctcttg ggaacaccct tgaggatttt tatcagatag ttatcaaggc gttgacccgc
     5761 ctcatgttcg gcaaccccta tcaagctgac cgaatctttg cttatttcgt gcgttttcat
     5821 ctataatccg aacatccgtt tcagcaaaaa gcgcgcccgc cgcgcccccc tgaaacggtt
     5881 tactttaaaa cggaatttta tattaaaaac gcactccgca aagcattttc cctgcgcctg
     5941 ttcccagccg gcaggcgcag aacgtagtca agtttgaatt gattttgccg tttcggcgcg
     6001 gaagtaagac ggcagccggg ccccaagtcc caaacgcaaa ccgcccaaaa cacaggcatc
     6061 acgataacaa gaagaagccg gcccccattt ttccgaccgc agcgttcgga acgcggcaga
     6121 caaaccgccg catcgatctt tatagccttc tgtatccgac ccttccgcac acggttctcc
     6181 ggaacgtgca tccctgcgga ttttcaactc aaaaactgat taccggtttt atccgaaaac
     6241 atcggaaaac ccagccttac ggaatgccga accggacagg tgcgcccgaa tgccgcccgt
     6301 ccacgaggtg atcatgaaaa gaatgttatt caacgcaacg caggccgaag agctgcgcgt
     6361 tgccatcgtc gacggacaaa acctgctgga tttggatatc gaaacgctgg gcaaagaaca
     6421 gcgcaaaggc aatatctaca aaggcatcat tacccgcatc gagccgtcgc tggaagcgtg
     6481 tttcgtcgat tacggaaccg accgccacgg cttcttgccg tttaaagaag tctcccgctc
     6541 atacttccaa gactacgaag gcggacgcgc gcgcatccaa gacgtgctca aagaaggcat
     6601 ggaagtcatc gtccaagtcg aaaaagacga gcgcggcaac aaaggcgcgg cgctgaccac
     6661 cttcatcagc ctcgccggac gctatctggt attgatgccg aacaacccgc gcggcggcgg
     6721 cgtatcccgc cgtatcgaag gcgaagaacg tcaagaactc aaagccgcta tggcgcaact
     6781 cgacattccg aacggcatga gcatcatcgc ccgtaccgcc ggcatcggcc gcagcgcgga
     6841 agagttggaa tgggatttga actatctcaa acaactctgg caggcgattg aagaagcagg
     6901 aaaagcgcat cacgaccctt acctgctctt tatggaaagc tcgctgctca tccgcgccat
     6961 ccgcgactat ttccgccccg acatcggcga aattctggtg gacaatcaag aagtttacga
     7021 ccaagttgcc gagttcatga gctacgtcat gccgggcaat ataggccgtc tgaaactcta
     7081 cgaagaccac acgccgctgt tttcccgctt ccaaatcgaa caccaaatcg aaagcgcgtt
     7141 ttcgcgcagc gtcagcctgc cctccggcgg cgcgatcgtc atcgaccata ccgaagccct
     7201 cgtctccatc gacgtgaact ccgcacgcgc cactcgcggc gcagacattg aagacaccgc
     7261 gttcaaaacc aatatggaag ccgccgaaga agtcgcccga caaatgcgcc tgcgcgactt
     7321 gggcggcttg gtcgtcatcg acttcatcga catggaaaac cccaaacacc agcgcgatgt
     7381 ggaaaacgtc ctgcgcgacg cgctcaaaaa agaccgcgcc cgcgtacaga tgggcaaact
     7441 ctcccgtttc ggacttttag aattgagccg ccaacgtttg aaaccggctt tgggcgaaag
     7501 cagccacgtc gcctgtccgc gctgcgccgg caccggcgtg attcggggca tcgaatccac
     7561 cgccctgcac gttttacgca tcattcaaga agaagcgatg aaggacaaca ccggagaagt
     7621 gcgcgcacaa gtgcccgtcg atgtcgccac cttcctgctg aacgaaaaac gcgccgagct
     7681 gtttgcgatg gaagagcgtt tggatgtcaa cgtcgtcctg attccgaaca tccacctcga
     7741 aaatccgcac tacgaaatca accgcatccg caccgacgac gtagaagaag acggcgaacc
     7801 gagctacaaa cgcgtcgccg agcctgaaga agacgaatcc gccaaaccgt tcggcggcga
     7861 aaaagccaaa gccgcccgtc ccgaacccgc cgtcaaaggc gtgcgccaca ccagccccgc
     7921 cccgactgcc gcccccgaga aaaaaacctc ctggtgggac agcttcaaag cttggctcaa
     7981 acgcattttc ggcggcagcg aaacccaagc cgcgcccgct gccgaaacct ccgaaaaacg
     8041 cagcacggca aaccgcagcg gcagccgcgc caacaaccgc cgccaaaacc cgcgccgcag
     8101 caaacgcgaa ggcagcaaag tagaagtccg cgaagtggca ggcaaaactg ccggacagga
     8161 agcgcgtgcc gacaaagccg aaacgcgcaa caacggcaac cgccgccgca acgaacgcgg
     8221 cgaccgtgcc gccggacgcg ccgacgaagc ggaaatccaa gaccgcaaca tacagcctgc
     8281 cgcaaccgtt gcagatgccg caccgtccga aaccgaagtg caaaccggaa aacgccgccg
     8341 caacggcagc cgcagcgaac gcggccaaac cgcgccggaa accgccaccg ttgccgaaac
     8401 aaccgttcag acagcggaaa acacgccgtc cgaaccgcat accgcagaag acaaaggcag
     8461 caagcccaaa tccgaacgca accgccgcga acgcgacagc cgagatgcca aagaacgccg
     8521 cgagcgcaac aatcagcgcg accgccgtca aaacggcaaa aaacgcaata ttccgtctgc
     8581 cgccaaaatc gagcagtacc tgaatattca cgacaccgcc gacaaagtcc gttccgccgc
     8641 cgcgcacgtt ttcggcgaaa ccgacgcaaa cgcgccgatt actgtcagca ttgccgatcc
     8701 ggttgcagaa agggatcttc cgacagcatc tcccgccgtt tcaaacggcg acgcaccggt
     8761 ttatgatgcg gcggaaaaaa tccgccgtgc caccgccgcc atcctgcccg aaggcgcgac
     8821 accgaaagcc gaagcacagg aaatgccgtc tgaaaccgca acctttacgg ctgcggcgga
     8881 acaggcacgg gaaaccgcac aaaccggcgg actcgtcctg atcgaaaccg accctgccgc
     8941 attgaaggca tgggcggcac aacccgaagt ccaagccgga cgcggtttgc gccgttccga
     9001 acagcccaag ccgtctgaag tcgcaaccgt ccctgccgaa gaaatgatcc aagtcgaaac
     9061 ccggcaaggc tgaaccgacg gcggcaaaaa gaggttctgt tccgcagaac ctctttttta
     9121 catgggttcg gatacctgca atgccgtctg aaacttcgcc attcccgtga ttaccgaaac
     9181 attccgccat tcccatgatt cccgcaacat tccgtcattc ccatgattcc cgcaacattc
     9241 cgtcattccc atgattcccg caacattccg tcattcccgt gaaaacggga atctagaacc
     9301 tcaaactttc ggataatctt tgaatattgc cgtcgcccaa aggcctggat tcccgcctgc
     9361 gcgggaatga cggcggaggg tggacgatgc cgtctgaaac ttcgccattc ccatgattac
     9421 cgcaaccttt cgt
//
LOCUS       DAWIVB010000064         9313 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWIVB010000064 DAWIVB010000000
VERSION     DAWIVB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.064"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(91..1371)
                     /locus_tag="WIX60_001093"
     CDS             complement(91..1371)
                     /locus_tag="WIX60_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ5305372.1"
                     /translation="MENSLKEAALKFHEFPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEIL
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1593..2213)
                     /locus_tag="WIX60_001094"
     CDS             complement(1593..2213)
                     /locus_tag="WIX60_001094"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ5305373.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLKPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRNLDEVRNSIEKVLDGHFGC"
     gene            complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WIX60_001095"
     CDS             complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WIX60_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ5305374.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3352..3924
                     /gene="ampD"
                     /locus_tag="WIX60_001096"
     CDS             3352..3924
                     /gene="ampD"
                     /locus_tag="WIX60_001096"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ5305375.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFAEAQYRSLEALLDAICRHYPVTAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFLVDRNAV"
     gene            4142..5428
                     /locus_tag="WIX60_001097"
     CDS             4142..5428
                     /locus_tag="WIX60_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ5305376.1"
                     /translation="MIYIVLFLAVVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5566..8091
                     /gene="ligA"
                     /locus_tag="WIX60_001098"
     CDS             5566..8091
                     /gene="ligA"
                     /locus_tag="WIX60_001098"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ5305377.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPVKWAENILAGIEASKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQTDKALPTDWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8148..>9313
                     /locus_tag="WIX60_001099"
     CDS             8148..>9313
                     /locus_tag="WIX60_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ5305378.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT         2183 a         2832 c         2357 g         1941 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactatatc aatctgacag caaaatgccg cctgaaaaca
       61 gttcagacgg cattttaaac cgcaaacggc ttacagcttc cattcgctca gcttggcagc
      121 gtaagcttcc aaatctgcaa tcggacgggt tgccacaccg ctttccatcg ctgctttggc
      181 ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac ggagtcggaa tcaggtattc
      241 cgcgccgaat tcaaatttct taccgtaagc ggcaaccact tcttcggtta cttcttccat
      301 cgccaaatcc gccaaagcat acacgcaggc gcgtttcatt tcttcgttga tggtcgtcgc
      361 gccgacatcc aacgcaccgc ggaagatgaa cgggaagcac aatacattgt tcacttggtt
      421 cgggaagtcg gagcggccgg taccgataac cacgtccgga cgggtttctt tcgccagcgg
      481 cggcaggatt tccggattcg ggttggccat agcgaacacg atgggttttt cgttcatggt
      541 gttcagtatt tcaggcgtca gcaggttcgc gccggagagg cccaagaaga tgtctttgcc
      601 tttgacggca tcggcaagca cgcgctggcc gttgtcttca atggcgtaga actgtttgga
      661 ctcgtccata cggtctttgt cttcgcgggt ttggtaaatc acgcctttgg agtcgcaaac
      721 ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg ttcaagcagg caatcgcggc
      781 cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt ttacggccgg taaaacgcag
      841 ggcgttcaat acggcggcgg cggtaatgat ggccgtgccg tgctggtcgt cgtggaatac
      901 ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag aaacactcgg gtgctttgat
      961 gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg gcgatgatgt ccacgagttt
     1021 ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc acaccggcga attttttgaa
     1081 caatacgcct ttgccttcca tcacgggttt gcccgccagc gcgccgatgt cgcccaagcc
     1141 caaaacggcc gtaccgttgg aaatgacagc gaccaagttg cctttggcgg tgtatttgta
     1201 ggcattttgc ggatcggcat ggatttccat acaaggagcg gctacgcccg gagagtacgc
     1261 caacgccaaa tctttgtcgg tcgccagaga tttggtcggg gtaacggaaa ttttgcccgg
     1321 cacggggaat tcgtggaact tgagggcggc ttctttcaat gagttttcca tgtgtcaatc
     1381 ctgttgcgag aaaaagattt gaatcggact gtccgaaacg ctttcgggtc atcatgttac
     1441 gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa ccgagcgtaa ttctatcatg
     1501 ccgaaagttt ggcgcatacc gccattttca cctgcggcac tgccgtttga ttttcggcgc
     1561 gggcggatgc gcttgtttca atatttgccg catcagcagc cgaaatgtcc gtccaacact
     1621 ttttctatgc tgtttctgac ttcatccaag ttgcggttac tgtcgataac ggcgtaccgt
     1681 tcgggacagg cggcggctcg gtcgagataa acgccgcgca cacgcataaa gaaatccgcc
     1741 tgctcctgct cgaaacggtc tttctcgcgc gtctgcccga tacgcgccat cgacacttcg
     1801 agcggcacat ccagcagcag ggtcaaatcc ggcttcaaac cgccctgcac ccaatgttcc
     1861 aaaatttcaa tgtcttcaga cggcatcccc cgcccgccgc cctgataggc gaaggtcgcg
     1921 tcggtaaaac ggtcagacac gacgtgtatg ccgtctgaaa gcgcgggcag gatgacttcc
     1981 tcgatgtgct gcatacgcgc ggcgaacatc atcagggttt ccgcacgcaa accggctttg
     2041 gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga ccggcgttcc gcccggctcg
     2101 cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg ccttgatgac ggcaaggttg
     2161 gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga attgcggttt catgcttgca
     2221 gttccggtaa gggtatgccg tccgaaaaca caaacttttc agacggcatg gtttattttt
     2281 tcaaaatata tttgcggacg gcggcattgt gttcggtcaa atcatggctg aactggctca
     2341 agcccgtgcc gtccattttg gacacgaaat acaggtattt ttcgccggac ggatgggcgg
     2401 cggcatcgag tgccgccttg ccgggcagcg caatcggggt tggcggcaga ccgccgcgcg
     2461 tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt acggattttg cccttgtatg
     2521 ccgcacccat gccgtaaatc acggacgggt cggtttgcag gcgcataccg attttcaggc
     2581 ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc atgccctgtt tccttttcga
     2641 ccaggctcgc cataatcagc atttcataag ggtttttata aggcagcccg tcctgcctgc
     2701 tttcccatgc ctcattcagg cggcgttgca tcgccttgta ggcggtttgg taaatctgca
     2761 aatcactgcc gcccgcatcg atttcgtagc tgtcggggaa aaactgccct tcaggattgc
     2821 cgctgaaggc atcgggcgca acttccgcca tcagtttttc attgctccag cctttggtgt
     2881 cgtgtccgat gtcgggcgtt gcgtcgatga ctttcctcat atgcgaaaaa cgcgaacctt
     2941 cgataatctg cacggtaacg gaatccggcc tgccgccgcg cattttctgc aagatatccc
     3001 aagcagacac ttccgaaggc aatctgtacg tccccgtatg cagcctgttg tgcacaccca
     3061 aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac gatgcggtct tcggcaagtt
     3121 tcctgccgac cgacgaaata ccctggtttt tggcaatttt gattcggtat gccctgccgt
     3181 tatccttagg aacaaaaagc agcgcggcga aaacggctgc cgacacggtc aaaaaaacgg
     3241 cagaccattt caacaatttt ctcaacatgg taggattccc agcattcgga atacgtctta
     3301 aaattcgggc gacagtataa cccaaattgc ccgccggaca ggaagaacat catggacaat
     3361 cacgccgaag cacactggca aaacggctgg cttcaaagca tacgccatac cccgtcgccc
     3421 aatttcagcc cgagggaaac gggggaaacg gtttccctga tcgtgttgca caacatttcg
     3481 ctgccgccgt tcgaatacgg cacggatgcg gtggaaaagc tgtttgccaa ccggctcgac
     3541 cccgacggac atccgttctt cagcctgata cacactttgc gcgtatccag ccatttctta
     3601 atcaaacgcg acggagaaac ggtacagttc gtttcctgcg acaatatggc ataccatgcc
     3661 ggcgtatctt cattcggcgg cagggaaaaa tgcaacgcat tttcaatcgg catcgaactg
     3721 gaaggctgcg atttcgaacc gtttgccgaa gcgcaatacc gttcgctcga agccttgttg
     3781 gacgcaatct gccgccacta ccccgtcacg gcggtaacgg ggcaccaaga catcgcgccc
     3841 ggccgcaaaa ccgaccccgg ccattttttc gactggcggc ggatacggga gaaagggttt
     3901 ctcgtggaca gaaatgccgt ctgaaaacca cagatttcag acggcatttc ggaaaatttc
     3961 cgcttatttc gacacactgc tgtcagaccc tgcaattttc gttataattc aaacgttata
     4021 atcggccgtt ccgtcagact gccggaaacg caagcccgat ttaaccgcag tgcaagcccg
     4081 atttaaccgc agtacccgca ctttgcgtat cctgcatccc gaaatttaac aagtgattgc
     4141 catgatttac atcgtactgt ttctagctgt cgtcctcgcc gttgtcgcct acaacatgta
     4201 tcaggaaaac caataccgca aaaaagtgcg cgaccagttc ggacactccg acaaagatgc
     4261 cctgctcaac agcaaaacca gccatgtccg cgacggcaaa ccgtccggcg ggtcagtcat
     4321 gatgccgaaa ccccaaccgg cggtcaaaaa aacggcaaaa ccccaagacc ccgccatgcg
     4381 caacctgcaa gaacaggatg ccgtctacat cgccaagcag aaacaggcaa aagcctcccc
     4441 gttcaaaacc gaaatcgaaa ccgccttgga agaaagcggc attatcggca actccgccca
     4501 caccgtttcc gaaccccaaa ccggacattc cgcaccgaaa cctgccgacg cgccggcaaa
     4561 acctgcaccc gttccgcaaa cacctgcaaa accgctgatt acgctcaaag aactgtcaaa
     4621 agtcgaatta ccctggtttg acgtgcgctt cgacttcatc tcctatatcg cgctgaccga
     4681 agccaaagaa ctgcacgcac tgccgcgcct ttccaaccgc tgccgctacc agattgtcgg
     4741 ctgcaccatg gacgaccatt tccagattgc cgaacccatc ccgggcatcc gctatcaggc
     4801 atttatcgtg ggtattcagg cagtcagccg caacggactt gcctcgcagg aagaactctc
     4861 cgcattcaac cgccaggtgg acgcattcgc acaaagcatg ggcggtcaga cgctgcacac
     4921 cgaccttgcc gcctttatcg aagtggcttc cgcactggac gcattctgcg cgcgcgtcga
     4981 ccagaccatc gccatccatt tggtttcccc gaccagcatc agcggcgtag aactgcgttc
     5041 cgccgtaacg ggcgtgggtt tcgttttgga agacgacggc gcgttccact ataccgacac
     5101 gtcgggctcg accatgttct ccatctgctc gctcaacaac gagccgttta ccaacgccct
     5161 tttggacaac cagtcctaca aaggcttcag tatgctgctc gacatcccgc actctccggc
     5221 aggcgaaaaa accttcgacg atttgtttat ggatttggcg gtacgcctgt ccggccagtt
     5281 gaacctgaat ctggtcaacg acaaaatgga agaagtttcg acccaatggc tcaaagacgt
     5341 gcgcacttat gtattggcgc gtcagtccga gatgctcaaa gtcggtatcg aaccgggcgg
     5401 caaaaccgca ttgcgcctgt tctcctaaaa tcgaatgcaa tcaaagcgga tatgtcgtta
     5461 cggcatatcc gctttttttc gggtaaaatt cagctgtttt caacctatca tttttcagac
     5521 gacctttgcc cgtatcagac aaaggtcgtc tgaaaacctt ctctcatgaa tccaaactca
     5581 aaacataaca ctaactttac caatcttctt aaaccgtctg atagtgatat aaaacaattt
     5641 gccgcacaac acatctgccg cctcaccgac ctcctcaacc gctacgccta cgaatactac
     5701 accctcgacg cgcccagcgt acccgatgcc gaatacgaca aattgttccg cgaactcgaa
     5761 gcgttggagt taaaccatcc cgagctcaaa ctgcccgaca gcccgaccca gcgcgtcggc
     5821 ggcgagcctt tggcgggatt tgccgaagta cgccacgaag tgccgatgct gtcgctgacc
     5881 aacgcttttt ccccgcaaga tgaaaacggc gtgttcgacc atgccgaaat gtacgccttc
     5941 gaccaacgcg tgcgcgacgg cttggacggc ggcaatcccg aatacgttat cgaacccaaa
     6001 ttcgacggcc tcgccatcag cctgctctac cgcgacggcg tattggtgca ggcggcaacg
     6061 cgcggcgacg gcacgacggg cgaagacgtt acccaaaaca tcaaaaccgt atccaacatc
     6121 cccttgcggc tgcacggcga aaatacgccc gaactcatcg aggtacgcgg cgaagtgctg
     6181 atgctcaaag ccgattttgt cgccctcaat aaaagacaag ccgaaaacgg gcaaaaaccc
     6241 tttgccaatc cgcgcaacgc cgccgccggc agcctacgcc aactcgattc gcgcatcacc
     6301 gcgcaacgca aactgcactt tttcccctac tccgtcgccc gccagcaaga tggtttcgtc
     6361 gcggaagaac acatccagga actcgcctat ttccaagcac tcggcttcag tctgcccaat
     6421 ggcaatttcg gctgtttcaa aaatatcgac gaagtattgg cgttttacga acacatgcag
     6481 caaaaacgcc ccgaactgcc ctacgaaatc gacggcatgg tcgtcaaagt caacagcttg
     6541 gcgcaacagc acgaactcgg cttcatctcc cgcgcgccgc gttgggcggt tgcccacaaa
     6601 ttccctgccg aagaagccct gaccattgtc gaagcgattg acgtgcaaat cggcaggacg
     6661 ggtgcggtaa cgccggttgc ccgattacag cccgtattcg tcggtggcgt aactgttacc
     6721 aacgccaccc tgcacaatca ggacgaagta tcgcgcaaag acgttcgcgt cggcgatacc
     6781 gtcgtcgtac gccgtgccgg agacgtgatt cccgaagttg tgcgcgtgat ttttgaacgc
     6841 cgcccgatgc gggaaaccgc cgttgccgtt tcagacggca tcgggcatcg gcaggacgat
     6901 ttgtttgccg aaacaccgtc cgccaatcaa acccaatccg ttccgctcca caagccctac
     6961 cgcctgccga cccactgccc catctgccgc agcgaaatcg aacgcgaaga aggcgaagcc
     7021 gtcgcccgat gcagcggcgg tatgctttgt caggcacaac gcgcgcaagg cttaatccac
     7081 ttcgcctcgc gcaaagcgat ggacatcgac ggcttgggtg aaaaacaaat cgaacagctt
     7141 gtcgcgcaag acctcgtccg ccacttcgcc gatctctacc gcctcgacat cccgaccttg
     7201 caaaaaatga aggaaacagc ggacaaaacc gttgccgaat cggatcaaat gccgtctgaa
     7261 ggcagttcgg tcggcgcgtc gggcaaacat aaaaaacaac cagtcaagtg ggcggaaaac
     7321 atcctcgcag gcatagaagc cagcaaaacg cccgaactcg cccgcttcct gttcgcgctc
     7381 ggcatccgcc acgtcggcga acgcaccgcc aaaacgctgg cacaggcatt cggcacattg
     7441 gaacgcgtcc gccgcgcccc cgaacccgtc ctcgcctgcc tgcccgacat cggtacggta
     7501 gtggcgcgtt ccattgccca cttcttcgcc caagccgaac agcaggcgat gatagacgaa
     7561 ctgctcgccg caggcgttgc cccgcaaacc caagccgtta ccatcccgcc cgcccgccac
     7621 gccgaaccgc aacgctggat cgcccgcctg cccggtttca aaatcagcga aaacaaagcc
     7681 caagccttat gggaactcgc cggcaaaaac atagaagggc tgcaaaccga caaagccctc
     7741 cccaccgact ggcaggcgtg gcgcagcgaa ccacaaaacg ccgccctgct cgaaaacctg
     7801 aaaaccttct tcgcgcaaat gccgtctgaa gacgaggcgg cgcaaggttc agacggcatc
     7861 aataaagccg tggcgggcaa aaccttcgtg ttaaccggca ccctgcccac cctcaaacgc
     7921 gaccaagccc aatccctaat cgaagccgca ggcggcaaag tttccggcag cgtgtccaaa
     7981 aaaaccgatt acgtcgtcgc cggagaagcc gcgggcagca agctggaaaa agccaatgcc
     8041 ttgggcgttt ccgtcctcag cgaagccgaa ctgcttaccc tgctcggctg aacccatgcc
     8101 gtctgaaccg ccttcagacg gcattgccca cttttccgaa ccacaccatg cacaccattt
     8161 cctttccaaa ccgcacccga ctcaccgccc tgccgcccct gtcgctttac atccacatcc
     8221 cgtggtgcat caaaaaatgc ccgtattgcg acttcaattc ccacagcctg aaaaacggat
     8281 tgcccgaagc cgcctatatc gacgcgctgc tgaccgattt gcagcttgaa ttgcccaata
     8341 tttggggcag gccggtggaa acgatctttt tcggcggcgg tacgcccagc ctgtttcagg
     8401 cggaatcgat tgaccgtttg ttaagcggcg tgcgttcgct gttgcgcttg cagccggaag
     8461 cggaaattac cttggaagcc aatccgggga catttgaaat cgagaagttt caaggattta
     8521 aagacgcggg tattacgcgg ctttctatcg gcgtacagag tttcaacgac gatatgctct
     8581 caaggctggg gcgcgtccac aacggtaagg aagccttaac cgctatcgat accgccttga
     8641 aattatttga taaagtcaat atcgacttga tgtatgccct gccgaaccaa accgttcaga
     8701 cggcattgga cgacgtacaa accgctatcg ctacgggcgc aacccacatc agcgcgtatc
     8761 acctgacgat ggaaccgaac acgccgttcg gtcatacgcc gccaaaaggt ttgccgcaag
     8821 acgaagccgc cctcgacatc gaagacgcgg tacacggcgc gctggaaggc gcgggttttg
     8881 tccactacga aacatcggct tttgcgaaac cagccatgca gtgccgccac aatttgaact
     8941 actggcagtt cggcgattat ttaggcatag gcgcgggcgc gcacggcaaa atttcctatc
     9001 ccgaccgcat cgagcgcacc gtccgccgcc gccaccccaa cgactacctc gccttaatgc
     9061 aaagccaacc gagtgaagcc gtcgaacgca aaaccgtcgc tgccgaagat ttgccgttcg
     9121 aattcatgat gaacgccctg cgcctgaccg acggcgtacc cgccgcgatg ttgcaggagc
     9181 gcacgggcgt accgagtgcc aaaatcatgg cgcaaatcga aacggcaagg caaaaaggcc
     9241 tgctggaaac cgaccctgcc gtattccgcc cgaccgaaaa aggacgcttg tttttaaacg
     9301 atttgctgca gtg
//
LOCUS       DAWIVB010000065         9106 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWIVB010000065 DAWIVB010000000
VERSION     DAWIVB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.065"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(44..3088)
                     /locus_tag="WIX60_001100"
     CDS             complement(44..3088)
                     /locus_tag="WIX60_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ5305379.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSHMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFHRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESQTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDA
                     WVVEPPEVPKVPMTAIDIQPPPPVSEIYNRTYEPPSGFEQVQRSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDT
                     EADEGAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLLPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3253..3933
                     /locus_tag="WIX60_001101"
     CDS             3253..3933
                     /locus_tag="WIX60_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305380.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMMGTSAALWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALTVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3962..4321
                     /locus_tag="WIX60_001102"
     CDS             3962..4321
                     /locus_tag="WIX60_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ5305381.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASIPPATGNLFANWIGA
                     FLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQLNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(4426..4656)
                     /locus_tag="WIX60_001103"
     CDS             complement(4426..4656)
                     /locus_tag="WIX60_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305382.1"
                     /translation="MNIFVTNFCYWFISLLAISYIPSINAWHDELIDDINFGKRVMMV
                     TFFAFLGTVIERFFKKKPWWFYPAKAFSLLQT"
     gene            complement(4835..5371)
                     /locus_tag="WIX60_001104"
     CDS             complement(4835..5371)
                     /locus_tag="WIX60_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ5305383.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5405..5761)
                     /gene="folB"
                     /locus_tag="WIX60_001105"
     CDS             complement(5405..5761)
                     /gene="folB"
                     /locus_tag="WIX60_001105"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ5305384.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            5817..6419
                     /gene="plsY"
                     /locus_tag="WIX60_001106"
     CDS             5817..6419
                     /gene="plsY"
                     /locus_tag="WIX60_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ5305385.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPVAASFF
                     MPHVSWVWATVAIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            6496..7341
                     /locus_tag="WIX60_001107"
     CDS             6496..7341
                     /locus_tag="WIX60_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ5305386.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPKLYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGSRYLL
                     DSKIR"
     gene            7587..8561
                     /locus_tag="WIX60_001108"
     CDS             7587..8561
                     /locus_tag="WIX60_001108"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ5305387.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANSAVAGLASEEEDTFVNAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(8704..8922)
                     /locus_tag="WIX60_001109"
     CDS             complement(8704..8922)
                     /locus_tag="WIX60_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ5305388.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2097 a         2701 c         2112 g         2196 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcggttgt agccgatacg caaggcgcgc tgtacgcccg
      181 aaatgctggc tttgcgcgtt ttcaggacaa cggatacggc ctcgtcgtac atcggatcgg
      241 tttcgtcgtc gccgctgcgc ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa
      301 tatcgtcaac atagtccggt tcgccaaact gtttcaaata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaacgcg ccgtgaacgc gctgcggata ggcagtaccc ggcagcagga
      421 acagcatatc gccctgaccg agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc
      481 tgtcgatttt gctggacact tggaacgcga tacgcgtcgg gatgttcgcc ttaatcagac
      541 ccgtgatgac atcgacgctg gggcgttgtg tggcaagaat caaatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cttcgatttt cttgcctgcc gtcatcatca
      661 ggtcggcaaa ctcatcgacc acgaccacga taaacggcag tttttccaaa ggttcgggat
      721 cgtcgggcgt gaggctgaac ggattgccga ttttttctcc ccttgctgcg gcttcggcga
      781 ttttttgatt gaagcccgca agattacgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg tgccggcaac caacaaatgc ggtgcttttc
     1081 ccaagtcggt tacgacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggcgaattga agatttcgct caggcgtatc atttggcgtt
     1201 tcgggttcgg aagttccaaa cccatgcagg ttttgccggg gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaaat ctttttccag attcagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaattac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgcctcgg gattgaacag cggaggcaga agcaggtcgg
     1501 ttgtcggcag gtgttcggat accgcaccgg tttcttcaga tgggaacgcc ccttcatccg
     1561 cttccgtatc cgatacccgg caggacgggc gttcagacgg cacattttca aacggacaaa
     1621 ccgcctgact gtcatgcccg aacgcttcgg tttccgacag atattgcccg cttgaccgct
     1681 ctgccgcacc ttcactgccg tcatccgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcggcaaga tggtcggtct cggcaatgcg gctgcgttgc acctgctcga
     1801 atcctgacgg cggttcatag gtacggttgt agatttccga tacgggaggc ggcggctgaa
     1861 tatcgattgc ggtcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcggcagg ttcttcaata tggatatcgg cagtttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacggtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtctga ctttccggaa tcaggcgaga aattcgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcgggca tcgcggacgg
     2161 ccgtgccgtc tgaaacggac tgcccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctttcccctg ccctgcatga cggtggaacg gcgcattccg tgcattttcg gctttgactg
     2281 cagaaaaggc agacggatgc tgcttctcaa aatacggctc aaatccgtaa tccgcagact
     2341 cggaaaacgc ttctttgtcc gcatcgaaca tatgggaata cgtgcgttga agcacagggt
     2401 cgtccaaacc gataatctgc agcccttcca tcggggtatc ggacacgcgg actttgggga
     2461 ccgctgtttc gtttttctca aatgcatcga tatagcgttt tttcgtttcc cttaaagccg
     2521 cgctgtttaa agcacgcgtt gcttcttcca aagtgatttc tttaaaaacc ggacggacgg
     2581 gcgaaatttc gctttcagac ggcatcaacc cttcagcaat actccggtcg aatgggatgc
     2641 ggcggttgtc gattacggca gttgcaatgt cttccgtatc ggcagcctct tcctccgcag
     2701 cttctgcttc ttccgtttcc gcttcttccg tcccgtttcc actgtctgaa tatccgtcgg
     2761 atgcggtcgg aacatccgca cggtttgcag aagcaggcgg aacgggcctg tccggttcgg
     2821 attcgtgcgc cagatagttt cggcagaaac ggacgacacc gacaaacagc caatacactg
     2881 ccgttttgac ggcatggaaa agcatcaggg caagttcggg aaaatcgggc ataccgtctt
     2941 tgatttcagg cagctccgcc tgtttttccc ctttcttttc ctgccacgca gaaacctcgc
     3001 gcatccactc gcgttcggat tgtgcgcgga caaaaaacaa gccggcaagc gcaagcaaca
     3061 aaataacgat caaaactatc caaaacatat gccgtttccc gcaagacaaa cgattaaacg
     3121 ggtttatttt aacgtgtttc catcccaaaa aaaacaaccg gccggcaatt ctgccgaaac
     3181 cgattcggct ttcggtttgc ccccgccgac agtataatag ccgtcccttc ccttaatctt
     3241 tgcggcattg ccatgatttt ccaaacagtc tggttttcag atatggtact gtcggtttcg
     3301 tggattgtcc ttatcctgat actggcagct tccgcgccgt cggcattccg ctcgcttgcc
     3361 cgctaccgtt ccgcccttcc cctgtgtacc gtaatttttt ccgcagcatg gtgtctgaac
     3421 gcctctgccg gcggcggaca gcttgcccag atgaactacc acctgctcgc cgtcaatttg
     3481 gttgcactga tgatgggcac ttccgccgcc ctttggcttg ccgcgctgct gatgctgccc
     3541 tactgcctgc tgtttgccgg ttctgtcggc gcatatccgc cgaacgcttt ggtgctgata
     3601 ctgcccgcgc tgaccgtcaa ccgcctgtcg cgtatgctgg tcaaccgcct gccgcccaat
     3661 attttcatct ttattttcgt caacggcttt ctcgcttccg ccgccggcat tctgctgacc
     3721 gggctggtgc tgaccggtat tttggatgcc gcaaacgctt ttccgtccga aatattgtgg
     3781 acgaccgccc tgcccgtctt tattctgctg gcgtgggcgg aagccttcct cagcggcatt
     3841 tcaactgcca tatttgttgc actgaagccc cattggatta acactttcga cgacaaccgc
     3901 tatcttaagt ccgaccgcgg catatggcgg tagccttcag acggcataga caggcaaaac
     3961 catgctttcc aacatcatcc ccctttccat cggtgcggca ctcggtgcaa cggcgcgttg
     4021 gctgctcaac ctcgccgttc ccgcatcaat acctcccgcc acaggcaacc tgtttgccaa
     4081 ctggataggc gcttttctta tcggaatctt tgccgaaacc gtcaaccacc cgcaatggaa
     4141 gctgctgctg attaccggtt ttctcggcag cctgaccacg ctttccggat tttcgctgga
     4201 aaccgtaacc ctgctccaat tgaaccgtcc cgcttcagca cttgccaata ttttcctgca
     4261 cacggcaggt tcgctgctgc tgacctggct cgggctgaag ataggggcgg cagtcaaata
     4321 accgcaacaa attgcctccc tttgttttga cataatctat aaattagctt cgcttgttta
     4381 aatactggct gtgccaatta ccgttctcaa attcaaattg agcttttagg tctgtaacaa
     4441 agaaaaagcc ttggcaggat aaaaccacca aggctttttc ttaaaaaaac gctctattac
     4501 cgtgcctaaa aatgcaaaaa aagtaaccat cataaccctt ttgccaaaat taatatcatc
     4561 tattaattca tcatgccatg cattaataga tggtatataa gaaatagcca ataaactaat
     4621 aaaccaataa caaaaattag taacaaagat attcattatt aaccatttta aaaattatca
     4681 cgttctttta ggtaattatc atcccctttt ctctcttatt actaaagcac gaatgacttt
     4741 aaaggacgcg cccagataca agccgaaaag gcatatccca tccgccaaac aaaccgcccg
     4801 ccgatccaat ccggcgggca agtccaacat cctgtcaatc cttcatcaac cagtattgca
     4861 aaccgattaa tgtcttgccg tctttaattt catcgtttgc caatgcctga cggacttctt
     4921 ctttcgacat caatacggtt tccgtaatct cgtcttcgtc attggcaagc gtactgccca
     4981 agcgcacgcc ttccgcctcg aacagataca ttttttcatt gcaaaaaccg accgccgtat
     5041 aaaagctgta cagcaggcgc acgctgtcgg caacataagg tgtttcctcc gccaattctc
     5101 gcagcgcaca cgctgccata tcctcgcctg ccacatccag cttgcccgca ggaagttcca
     5161 aggtcgcctg atttgccgca taacgccact gccgcaccaa aacgaccttt tcatcatccg
     5221 ttaccgccaa cacgcacgcc gcgcccggat gacggatgac gatacgctgc ccttcattgc
     5281 cgttgggcaa cctgacctta tccctgctga tactgacgaa accgccctcg taaatggttt
     5341 cgccgcctaa ttttacttcc ctcaaatcca cttcatatct cctttccgtt tcctattctg
     5401 ccgttcaatc ttcacgcttg ccgcgctcga tttccacgcc aacctcgcgc acgccttcca
     5461 aaatacccgg cttgacgatt ttcacgcgca cccacaccgc gccgaaatat cccaaaacca
     5521 aatcggcaat atattccgcc aacgcttcca aaagcaggaa atcctgttct ttcagatgtc
     5581 ggcgcaatgt ttcgcatacc tcggcataat gcaccgtatt ggcaatatcg tcgtccgaac
     5641 ccgctttctc gggaacgccg atgtccaaat cgacaatcag ggtctgcaac cgttcgcgtt
     5701 cccagccgta cacgccgata agcgtatccg ccttgatgcc gtgcaaaaag attttatcca
     5761 taatccgccc cgttttttgc taaaataacc gcaccattct aagtaaagta ccgcaaatgt
     5821 tcaacatacc ggctgttgct gtttcctatc tgatcggctc actttctttc gccgtcatcg
     5881 tgtccaagta ttacggcatg gacgacccgc gcacctacgg atcgggcaat ccgggggcaa
     5941 ccaatgtttt acgcagcggc aaaaaaaagg cggccgcgct gacgctcttg ggcgatgccg
     6001 ccaaaggttt agttgccgtt ttgcttgcac gcgtgcttca agaaccgctc ggtttatccg
     6061 acagcgcaat cgcggccgtc gcactcgccg cgctggtcgg gcatatgtgg ccggtgtttt
     6121 tcggatttaa aggcggcaaa ggcgtggcaa cggcattggg cgtgcttctg gcactctctc
     6181 ccgcaactgc cttggtctgc gcgttgattt ggcttgttat ggcattcggc ttcaaggtgt
     6241 cctcccttgc cgcattaacc gccacaatcg ccgcaccggt cgccgcatcc ttctttatgc
     6301 cgcacgtctc gtgggtttgg gcgaccgtcg ccattgcttt gctggtgttg ttccgccaca
     6361 aaagtaatat cgtcaagctg ctcgaaggca gagaaagcaa aatcggcggc agccgctgat
     6421 ttcccatcca tccctgttta tgccgtctga accccgcttc agacggcatc cgaatatccc
     6481 gccgttttac cgactatgct cagttacaga cacgcattcc acgccggcaa ccacgccgat
     6541 atgctcaaac atttcacctt gtttttagtg ctgcaatatt tcaaccgcaa agacaagccc
     6601 tactggtaca tcgacacgca cggcggcgcg ggtgtgtaca atctcgaagg cagcgaggcg
     6661 cagaaagtcg gcgaataccg acaaggcatc gccctgctcc gacaggcgca aaacctgcct
     6721 gccgaactct ctgactttgc cgcgcacata caaaaaatcc tgccctcgcc caaactttac
     6781 tgcggttctc cgtggctggc gcaatcgctg acccgcgtcg gcgacaaatt acgcctgttt
     6841 gagctgcacc ccaccgactt tgtccatctg caaaacaata tgggcgaagc aggattgggc
     6901 aaacgcggac aagtattacg cgaagacggt tacaaagggc tgatttccct gctgccgccc
     6961 ccgccgcgcc gcgccaccgt cctcatcgac ccgccctatg aggaaaaaca ggactaccgg
     7021 cgtgtaacgg aaacgctgaa agccgcttta aaacggtttg agtccggctg ttatctcatt
     7081 tggtatccct gcctcagccg cgaagaaagc cgcaagctgc ccgaagaatt gaaaaaactc
     7141 ctgcccgaca actaccttca cgccgaactt cacgtccacg cgccgaaagc cgacggtttc
     7201 ggtatgcacg gcagcggaat gttcgtcatc aatccgccct atctgttggc ggaacaattg
     7261 gcggcaaacc tccccgccct gacgcggctg ctggcgcaag acgaaggctc gcgctacctt
     7321 ttggacagca aaatccgctg accccgatgc cgtctgaaac ccttcagaca acatctcccg
     7381 tcaaataaac aaaaccggac gatataccgg cagtcggcac ttttaagaat aaatatcccc
     7441 tcatccgcct gccgcgttcc gacttgcccc atgccgtttt tttacactac aatcccctag
     7501 actatcctgc aaaacgcacc aaaggaatac gccacaataa cctgccgcct ttcagacggc
     7561 acaaaccttt ccgaaacgag caaactatgg acacactgac ccgattcctc cccgaacacc
     7621 tgcaacaaaa ccagctgccc gaagcactcg gcggcgtttt actgtccgtt gtttccgcct
     7681 gcaccgaaat caacgccaaa gtccgcctcg gcgcactggc cggcgtattg ggtatggcgg
     7741 gcacgggcaa tattcagggc gaagaccaaa aaaaactgga tgttatcgcc aacaacatca
     7801 tgattgacac actcaaagcc aattctgccg ttgccgggct ggcgagcgag gaagaagaca
     7861 ctttcgtaaa tgccggtgaa aacggacgct atctcgtcct attcgaccct ttagacggat
     7921 cgtccaatat tgatgtcaac atttccgtcg gtacgatttt ctccatcctt gaaaaacccg
     7981 aaggcgcatt ggcaaccgaa tcattccttc aaacgggcag acagcagctt gccgctggtt
     8041 acgttcttta cgggcctcaa acccagctcg tattcacatt cggacatggc gtatatatgt
     8101 tcacgctcaa tgccgaaaac gaatttgtgc tgaccaaaga aaacccgaaa gtacccgaaa
     8161 gtaccaaaga atttgccatc aatatgtcca accgccgcca ctggctgccc cccgtccaac
     8221 aatacatcga cgagctcttg gcgggcgaaa ccggtacgcg cggcaaaaac tacaatatgc
     8281 gctgggtagc cagtatggtt gccgaaatcc accgcatcct gatgcgcggc ggcgtgttca
     8341 tgtatccgca agacaaacgc gaccccgcta aacccggcaa actgcgcctg atgtacgaag
     8401 ccaaccctat gagcctgata ctcgaacagg cgggcgcatc ggcaagcaac gcatatcaag
     8461 ccatgctcga catacagccc gaaaacctgc accaacgcgt cgccgtcttc atgggcagca
     8521 gcgaagaagt cgcctatctc gaccgcctgc acgcaaaata gacagacagc cgttcacaca
     8581 agtacacggc ggcggcaatc cgataccgcc gccgttattt caaacagcac aagccttccg
     8641 gtcaaatgac aaaaatgccg tctgaacaca tttcagacgg catttcaaaa actcaaaccc
     8701 gaattatttt cccgctgctt tttgcaggat ggcgtacaac tcgtctttca gtttcaattt
     8761 ggcttttttc agctcatcga tggtttccgc accgctggta accggattgt tgaccagacc
     8821 ggtaatttta tcgtccagct cgttgtgttc gtcgaacaga cgggcgaagc gggaattttc
     8881 ctgtttcaat ttggaaatca aatcacggta ttctgggaac atatcgcttt cctctcttgg
     8941 tttacggtta aaaagtcaaa ttgaatcttt acattaatta taacaaagat aatattttta
     9001 tccagtgttt tatcggatac ggcaaaattt tcttttaaca ccgccgtccg gttgataaaa
     9061 acgttgacaa tatcacgctt tacgcataaa ttttcagcct ttccta
//
LOCUS       DAWIVB010000066         8851 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWIVB010000066 DAWIVB010000000
VERSION     DAWIVB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.066"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(175..867)
                     /locus_tag="WIX60_001110"
     CDS             complement(175..867)
                     /locus_tag="WIX60_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ5305389.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="WIX60_001111"
     CDS             complement(975..2168)
                     /locus_tag="WIX60_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ5305390.1"
                     /translation="MSNQKNRKNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WIX60_001112"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WIX60_001112"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ5305391.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIANIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="WIX60_001113"
     CDS             complement(3021..3950)
                     /locus_tag="WIX60_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ5305392.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKL
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGKLAQVRFGSK
                     VKFKIIGLKKATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WIX60_001114"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WIX60_001114"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ5305393.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="WIX60_001115"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="WIX60_001115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ5305394.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVNVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASQEQGVSLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMPLPEIIAGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNATLESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEESRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7220..7678
                     /locus_tag="WIX60_001116"
     CDS             7220..7678
                     /locus_tag="WIX60_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ5305395.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGAMGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRECGRDGEPKVVFVEQESDF"
     gene            complement(8003..8164)
                     /locus_tag="WIX60_001117"
     CDS             complement(8003..8164)
                     /locus_tag="WIX60_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305396.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPKLV"
     gene            complement(8287..8645)
                     /locus_tag="WIX60_001118"
                     /pseudo
     CDS             complement(8287..8645)
                     /locus_tag="WIX60_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
     gene            8698..8835
                     /locus_tag="WIX60_001119"
                     /pseudo
     CDS             8698..8835
                     /locus_tag="WIX60_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2220 a         2434 c         2188 g         2009 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcgac gtattcgggc gtgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaaaccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt aatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tacctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg cgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttttgcg gtttttttga
     2161 ttagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg tccactgctt tgacctgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccatc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttgat
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatattggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 taggcttggt ttttgcgccg cagggcggtg gcttttttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ttgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagataatcg cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtacttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccagttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt gcccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggtg aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggcgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catccctatg tttcccgacc aatccgcccc
     4801 caacctcctg caaggcttga atcccgaaca actctccgcc gtaacctggc cgccgcaatc
     4861 cgcccttgtg ctggcgggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgcatcgc
     4921 atggcttttg caaagcggac aagccagcgt gcatagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc agacccgttt gggcgcaatg attcccgtca acgtccgcgc
     5041 catgtggctc ggcacgttcc acggcctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgcaggcctg ccgtcttcct ttcaaatcct cgacagcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggttt gcgcgcttcc gtcttgagcg cgcccgaccc
     5281 gcacacaagc cgcatgatcg aatgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat ttcgccgagc ttatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccag cattaccaaa accgcttcaa ccacattctc gtcgacgagt tccaagacac
     5461 caacaaactg caatacgcct ggctcaaact catggcgggc ggaaacgcag cggtatttgc
     5521 cgtcggcgac gacgaccaaa gcatttaccg tttccgtggc gcaaacgtcg gcaacatgac
     5581 cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaac atccttgccg ccgccaatgc cgttatcgaa aacaacgacg aacgactcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatt cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcgtgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg tagcaacgcc caatcccgcg ttatcgaaca
     5881 aagcctgttc cgcagcggca tcccctacaa aatctacggc ggcttgcgct tttacgaacg
     5941 ccaagaaatc aaacacgccc tcgcctacct gcgcctcgcc gtcaatcccg acgacgacaa
     6001 cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggtgcacgta ccgtcgaaaa
     6061 tcttcagacg gcatcgcaag agcaaggcgt ttcactttgg caggcagcct gcaatgccgg
     6121 cgcaaaagcc gccaaagtcg ccgccttcgt ccgcctcatt gaagccctgc gcaaccaagt
     6181 cggacaaatg cccctgcccg aaatcatcgc cggcatcctc aaagacagtg gcttgaccga
     6241 acactaccgc acccaaaaag gcgacaacca agaccgtctc gacaaccttg acgaactcgt
     6301 caacgccgcc atcgagttca aacccgaaga cagcaacttt gaaaccctgc ctgaaaacat
     6361 ttcagacgat cccgccttcc ccatcctcgc tttcttgagc aacgccaccc tcgaatccgg
     6421 cgaaaatcaa gcaggtgcag gcgaaaaagc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa tttaacgccg tcttcctcac cggcatggaa gaaagccgct tccccagcga
     6541 aatgagtctc gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcgcaa caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagagatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagcctg cctacgacag ttacggcaac acgcgccaaa ccgccgcatc
     6781 caaagataaa atcatcgacg actacaaaca gccccaaacc tacgcaggtt tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg cagataaagg
     6901 cgaatccgcc cgactgacca tcaatttcgg caaacagggc gtgaaagagt tggacaccaa
     6961 gtttgcgaaa ttggaagaga tgtagaaggg agatggcaga ggccgtctga aaacgggaac
     7021 aacaattttt gcccaaacct ccgccgtcat tcccgcgcag gcgggaatcc agacctttcg
     7081 gcacgggaac ttatcggata aaaggtttct ttagattcca cgttctagat tcccgcctgc
     7141 gcgggaatga cggacggtaa aagccgggta tagataccta acccttgttt atagctgaaa
     7201 atcaataagg aagaacatta tgtcccaagt ttttaaagat tttgatttgt cctccgtatg
     7261 ggaatccgac agttgggcag atgaaaacta caaagaagcc ccgtttaccc ctgaaatttt
     7321 ggctgccgta gaaagtgaac tgggctataa attgccgcaa agttttattg aattgatggc
     7381 ggtacaaaac ggcggaatat ttgtcaaaaa ctgttttccg accacacaga gaaattcgtg
     7441 ggcgaaagat catgtgcaaa tttgcgaggt atcgggaatc ggttttgaaa aagaagggag
     7501 tttgtgcggc gcgatggggc aaaaactttg gctggaagaa tgggaatacc cgcctatcgg
     7561 cgtgtatttt gccaacgacc cgtcaggcgg tcatgccatg tttgccttgg actatcggga
     7621 gtgtggaaga gacggagagc cgaaagtggt gtttgtcgaa caagaatcgg atttttaaat
     7681 cgtcgaactt gcccccgatt ttgaaacctt tattcacagc ttgcggcatg aagatgagtt
     7741 tattgacgag gaaatataaa acggcggttg aaaaaccaga atcatcaaag gaaaacgggc
     7801 ggaataacag ataaccgatt gaatccgtaa ggaaaacggt ttgtaggaac gtgccatcca
     7861 agacctttgc aaagaaaaac tgtccccgac agcattgaca ttattaacag aacttatcaa
     7921 ttttggagct atctcaaata taattcggtt atcctgttgt atccattaaa tcatatgctt
     7981 caattaaatg ttgttctagc tcttatacca attttggatt gcgaattcct gatacaatct
     8041 caaattcttc tgcatctatg caaacacctg cataaatttc aataacaagg gaacgcaata
     8101 attgaagctc ttctcttgtt aaagaaataa taatgtcatc acctgtgtaa ttgattatat
     8161 tcataataat tttatttttg cttgtcaaag taagttttgc ctaaggttgg tctcaatgct
     8221 tcccaccatc ttttgaattt gggtctctga ttacaaccgt tccagactta tcatcccaaa
     8281 ttgctcttat gtgtttggat tgtaatcttc gaattcccaa gaaaaaaatc gtaataagtt
     8341 tgaaagtgtc aaatcccaag tttcttttga gcaatattct aatattttat caatttcact
     8401 tttaataatc ttatgatcaa actgttctaa tattaatgca ttagaccaaa aaaaccttct
     8461 ttattacaat gatgggaaat ccatttagga gaacaaatgc aaagtgaaaa aatagatgag
     8521 ccttgttctc cttcgattcc gatatccaaa tctatccatc tatggaaatt atctggaatt
     8581 tcgggggtaa atttttcaaa atcaatatca tataaattta tgctttttaa atccaattta
     8641 atcattaggg ctgtcctaga taaataggga aattcaaatt aagttagaat tatccctatg
     8701 agaaaaagtc gtctaagccg gtataaacaa aataaactca ttgagctatt tgtcgcaggt
     8761 gtaactgcaa gaacagcagc agagttagta ggcgttaata aaaataccgc agcctattat
     8821 tttcatcgtt tacgatgact taatttatca a
//
LOCUS       DAWIVB010000067         8846 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWIVB010000067 DAWIVB010000000
VERSION     DAWIVB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.067"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(134..469)
                     /locus_tag="WIX60_001120"
     CDS             complement(134..469)
                     /locus_tag="WIX60_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ5305397.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAATKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            598..1503
                     /gene="mtrA"
                     /locus_tag="WIX60_001121"
     CDS             598..1503
                     /gene="mtrA"
                     /locus_tag="WIX60_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ5305398.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1565..2053)
                     /locus_tag="WIX60_001122"
     CDS             complement(1565..2053)
                     /locus_tag="WIX60_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ5305399.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2200..3102
                     /locus_tag="WIX60_001123"
     CDS             2200..3102
                     /locus_tag="WIX60_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ5305400.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3143..4273)
                     /locus_tag="WIX60_001124"
     CDS             complement(3143..4273)
                     /locus_tag="WIX60_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5305401.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4478..7102
                     /gene="alaS"
                     /locus_tag="WIX60_001125"
     CDS             4478..7102
                     /gene="alaS"
                     /locus_tag="WIX60_001125"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ5305402.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERIAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALVKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPAVLDSVKDWVGAKLV"
     gene            7363..7794
                     /locus_tag="WIX60_001126"
     CDS             7363..7794
                     /locus_tag="WIX60_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305403.1"
                     /translation="MSISLIGLHITIAIILFFTTNFMGKKSSIFGYYQLSFSEENHSP
                     AFNIFYRAFTPILFIVIFSWVVTSLEIPISLEKINYVVIYYFIIRLLSVFVFEKTHIV
                     NWFNQLTIPILSITLSFIVYNKMILPKSFLLPSSQEVATTF"
     gene            8094..8381
                     /locus_tag="WIX60_001127"
     CDS             8094..8381
                     /locus_tag="WIX60_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305404.1"
                     /translation="MQVTSAEYLSNEESIKKGGNILMEKYNEKYNESIDGNKTLYKSY
                     YESRRESIKNYNPDAKYINEIESIYMMLGEIYPNAPDFMSPHFEGDCSEGE"
     gene            8580..8666
                     /locus_tag="WIX60_001128"
                     /pseudo
     CDS             8580..8666
                     /locus_tag="WIX60_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
BASE COUNT         2300 a         2252 c         2082 g         2212 t
ORIGIN      
        1 ttcacgctta ccccttgttt ctcaaatgcc gtctgaaata agcggcttaa tatattgttt
       61 acagtattgg gaagcataac agacaaaatg ccgtctgaaa tattttcaga cggcatttct
      121 tatccgaaac ggattatttt tgcgtttcaa ccgcttccaa tgcacgcagg gcataagtgt
      181 aagcggcacc cgcattcagg gcaatggcgg ttgccaatgc acctgcgatt tcgctgtcgg
      241 tcgcaccggc tttggtggcg gcggcggcgt gaacactgat gcagctctca caacgtgtag
      301 taatggcaac ggcgatggca atcagttcgc gtgttttagc atcaagtgcc tctgcagctg
      361 ccgcttgttc caatgcgccg taggcctgca gcattttagg atgcgcctta cccagctcgc
      421 cgaacgattt tttaaccaat gcggtatgtt ctttccaatc tttaaacatt ttcttttcct
      481 ttctcttgcg tttaaccctg atacgcgctt gcgtatctgt tttcgatgtg cgtattattg
      541 caattattca gttgtgtttc tcgtttaatc atctcatttt atggttcaaa aagatttatg
      601 gacattctgg acaaactggt cgatttcgcc caattgacgg gcagtgtgga tgtgcagtgc
      661 cttttgggcg gacaatggtc ggtacggcat gaaaccttgc aacgcgaagg attggtacac
      721 attgttacat cgggcagcgg ctatctctgc atcgacggcg aaacttcccc gcgtccggtc
      781 agtacagggg atattgtatt tttcccgcgc ggcttgggtc atgtgttgag ccacgacgga
      841 aaatgcggag aaagtttaca accggatatg cggcagcacg gtgcgtttac ggtcaagcag
      901 tgcggcaacg gacaggatat gagcctgttt tgcgcccgtt tccgctacga cacccacgcc
      961 gatttgatga acgggctgcc tgaaaccgtt tttctgaaca ttgcccatcc gagtttacag
     1021 tatgtggttt caatgctgca actggaaagc aaaaaacctt tgacggggac ggtttccatg
     1081 gtcaacgcat tgtcgtccgt cctgctggtg cttatcctgc gcgcctatct cgaacaggat
     1141 aaggatgtcg aactctcggg cgtattgaaa ggttggcagg acaaacgttt gggacattta
     1201 atccaaaagg tgatagacaa accggaagac gaatggaatg tcgacaaaat ggtggcggct
     1261 gccaatatgt cgcgcgcgca actgatgcgc cgtttcaaaa gccgggtcgg actcagcccg
     1321 cacgcctttg tgaaccatat ccgcctgcaa aaaggcgcgt tgctgctgaa aaaaaacccg
     1381 gattcggttt tgtcggtcgc actgtcggta ggctttcagt cggaaacgca cttcggcaag
     1441 gcgttcaaac ggcaatatca cgtttcgccg ggtcaatacc ggaaagaagg cgggcaaaaa
     1501 taaatcgggg cttcaaacgc aaatgccgtc tgaaaaggct ttcatacagc atttgcgtac
     1561 cgcgtcattt caagggctgc atcttcatca cttccatcaa aaagttggta aatgcggggt
     1621 tgttgggttt gacatccata tttttccaac gctgctgcca gccgcgcaag gcattctgga
     1681 tatacagctt ggactgttcc gtattgattg cgccccgctg gctgtctatc gccgaacgca
     1741 ggtagatttc atacatactg tcatcgacgg cattgcgtcc gaccaggcgt tttctgaagt
     1801 tgttcagata ttgcgccgcc tgaaccttgg tcattttacc gatacccacc tgatagccca
     1861 agcgcgtcgc ttcatcgctg attttggcaa catccgtcca atgcgaagag gcaaggcgga
     1921 aaccttttgc aggtgcttcc gttttgacgg tattgatagg attcacgggg atttccgtca
     1981 atgtgggcac ataaatagac tggcagccgg aaagaactgc cgcaatggaa agagggataa
     2041 ggtatttttt catgccccca ttataatcaa gtttgccttg agaaaacaaa ttgttcggca
     2101 agaaaaataa aatttcggca tcagaagcag gcaaaaacac attccacaag ccttgccgca
     2161 aggtttacaa tccgaccgtc cttatcgcaa cgaccgttta tggataccgc aaaaaaagac
     2221 attttaggat cgggctggat gctggtggcg gcggcctgct ttaccattat gaacgtattg
     2281 attaaagagg catcggcaaa atttgccctc ggcagcggcg aattggtctt ttggcgcatg
     2341 ctgttttcaa ccgttgcgct cggggctgcc gccgtattgc gtcgggacac cttccgcacg
     2401 ccccattgga aaaaccactt aaaccgcagt atggtcggga cgggggcgat gctgctgctg
     2461 ttttacgcgg taacgcatct gcctttggcc actggcgtta ccctgagtta cacctcgtcg
     2521 atttttttgg cggtattttc cttcctgatt ttgaaagaac ggatttccgt ttacacgcag
     2581 gcggtgctgc tccttggttt tgccggcgtg gtattgctgc ttaatccctc gttccgcagc
     2641 ggtcaggaaa cggcggcact cgccgggctg gcgggcggcg cgatgtccgg ctgggcgtat
     2701 ttgaaagtgc gcgaactgtc tttggcgggc gaacccggct ggcgcgtcgt gttttacctt
     2761 tccgtgacag gtgtggcgat gtcgtcggtt tgggcgacgc tgaccggctg gcacaccctg
     2821 tcctttccat cggcagttta tctgtcgtgc atcggcgtgt ccgcgctgat tgcccaactg
     2881 tcgatgacgc gcgcctacaa agtcggcgac aaattcacgg ttgcctcgct ttcctatatg
     2941 accgtcgttt tttccgctct gtctgccgca ttttttctgg gcgaagagct tttctggcag
     3001 gaaatactcg gtatgtgcat catcatcctc agcggtattt tgagcagcat ccgccccact
     3061 gccttcaaac agcggctgca atccctgttc cgccaaagat aaaaaatgcc gtccgaacat
     3121 ccttcagacg gcatatcggg ctttatttcc ccgccttcac atcctgccac tggcgcacca
     3181 taaacttcaa tgccgccggc tggataggca ccatgataaa gctgtttttc aaatcctcct
     3241 cggttgggaa aatcgtattg tcgtttttaa attcgtcttc catcagctca cgcgcaggct
     3301 tgctcgaagg cgcgtaagta acgaaattgc cgtttttcgc cgacacttcc gggtcgagga
     3361 agtcgttgat gtatttgtgc gcgttggcga cgtttttcgc atctttcgga atcacgaaag
     3421 aatccaccca aatccccacg ccctctttgg gcatcatcac gcggattttt tccttgccgc
     3481 ccgcttcttc ggcacggcgt ttggcgatgt tcaaatcgcc gccgaaaccg attgttacgc
     3541 aggtatcgcc gcgcgccaaa tcatcgataa agccggacga agtaaagcgt ttgatattgg
     3601 ggcggttttt cttgagtagg gcggttgcct ccctgatgtc ttccgtattg ctgctgttcg
     3661 ggtttttacc caaatagttc aacaccatag gatagatttc cgccgcgctg tccaaatagc
     3721 tgatgccgca ttgcttgagt ttggacgtgt attcggggtc gaacaccaaa tcccactggt
     3781 tgtccggcag cttgtccgta cccaaagcct ttttcacgcg ttcggtattg atggcgaagg
     3841 tatttgtccc ccaataaaac ggcacggcgt attcgtggcc gggatcgacc ccgtccatca
     3901 gcctcatcat ttcggggttg aggtgtttat aattgggaat cagcgactta tcgattttct
     3961 gatacgcacc tgccttaatc tgcctgccca caaacgcatt ggacggcgcg acaatgtcgt
     4021 aaccggactt gcctgtcagc accttgcttt ccagcgtttc atcgctgtcg tacacatcat
     4081 aagtaacctt gatgccgttt ttcttttcaa aatcggcaac ggtttccgga tcgacatatt
     4141 ccgaccagtt gtaaattttc aatacgtttt ggttttccgc cggtgccggt ttttcggcag
     4201 gcggtttgtc cgaaccgccg cacgctgcaa gcagcaaagc agtcaggacg gccaggggca
     4261 gatgtttggt cattatcatt ccttgcatat cgggttggag aaagcggcca ttatagccga
     4321 tattggcaac agggcttcag acggcattca aaatcccgcc acactcttcc gaaaaccgcc
     4381 gcttccatag ctagaaacag ggatttgcgg taagataccg ccgttcgttt tccctgcttt
     4441 taccatgaca agacatttga gagacattga aaaaattatg aaaacctccg aactgcgcca
     4501 aaaattccta aaattttttg aaaccaaagg ccacaccgtc gtccgctctt ccagcctcgt
     4561 gccgcacgac gacccgaccc tgctgtttac caacgcgggc atgaaccagt ttaaagacgt
     4621 attcttaggt ttcgacaaac gcccgtacag ccgcgccacc accgcgcaaa aatgcgtacg
     4681 cgcaggcggc aaacacaacg acttggaaaa cgtcggctac accgcccgcc accacacctt
     4741 ctttgaaatg atgggcaact tctccttcgg cgactacttc aaacgcgacg ccatccactt
     4801 cgcttgggaa tttctgactt cccccgaatg gctcaacatc cctaaagaca aactgttggc
     4861 gaccgtttac gcggaagacg acgaagccta caacatctgg ttgaacgaaa tcggtatgcc
     4921 gtccgagcgc atcgtccgca tcggcgacaa caaaggcgcg aaatacgcat ccgacaactt
     4981 ctggcaaatg ggcgacaccg gcccttgcgg cccctgctcc gaaattttct acgaccacgg
     5041 cgaagaaatc tggggcggca ttcccggcag tcccgaagaa gacggcgacc gctggatcga
     5101 aatttggaac tgcgtattta tgcagttcaa ccgcgacgaa caaggcaata tgaacccgct
     5161 tcccaaacct tccgtcgata ccggtatggg cttggaacgc atagccgccg tcatgcagca
     5221 tgttcacagc aactacgaaa tcgacttgtt ccaagacctg ctcaaagccg ttgcccgcga
     5281 aaccggcgcg ccgttcagta tggaagaacc cagcctgaaa gtcatcgccg accacatccg
     5341 ctcctgctcg ttcctgattg cagacggcgt cttgccttcc aacgaaggcc gcggctacgt
     5401 attgcgccgc attatccgcc gcgccgtgcg ccacggttac aaactgggtc aaagcaaacc
     5461 gttcttccac aaactcgttg ccgatttggt caaagagatg ggcggtgcct accctgaatt
     5521 gaaagaaaaa caagcccaaa tcgaagaagc attgaaaaac gaagaaagcc gttttgccca
     5581 aacgctggaa accggtatgg ctttgttgga aaacgcgctg gtcaaaggcg gcaaaacact
     5641 cggcggcgaa atcatcttca aactctacga tacctacggt ttcccatacg acttgactgc
     5701 cgacatctgc cgcgaacgca atatcgaacc ggacgaagca ggcttcgagc gcgaaatgga
     5761 agcccaacgc gcacgcgcac gcgccgccca aagcttcaaa gccaacgccc aactgcctta
     5821 tgacggtcaa gacaccgagt ttaaaggtta tagcgaacgc caaaccgaat ccaaagtcct
     5881 cgccctctac aaagacggcg agcaagtcaa cgaattgaac gaaggcgaca gcggcgcagt
     5941 cgtcatcgac tttaccccgt tctatgcaga atccggcggc caagtcggcg atgtcggcta
     6001 tatcttctca ggcgaaaacc gctttgaagt acgcgatacc caaaaaatca aagcggccgt
     6061 attcggtcaa ttcggcgtac aaacttcagg ccgtctgaaa gtcggcgaca gcgttaccgc
     6121 caaagtggac gacgaaatcc gcaatgccaa tatgcgcaac cacagcgcaa cccacttgat
     6181 gcacaaagcc ctgcgcgatg tattgggcag acacgtcgaa caaaaaggct ctttggttac
     6241 cgccgaatcc acccgtttcg acatttccca tccccaagcg gtaactgccg aagaaattgc
     6301 cgaagtagaa cgccgcgtca acgaagccat tttggcgaac gttgccgtca atgcagccat
     6361 tatgagcatg gaagacgcgc aaaaaaccgg cgcgatgatg ctcttcggcg aaaaatacgg
     6421 cgaagaagtg cgcgtactgc aaatgggcgg tttctctacc gaattgtgcg gcggcacaca
     6481 cgtttcacgc accggcgaca tcggcctctt caaaatcatc agcgaaggcg gtattgccgc
     6541 aggcgtgcgc cgtatcgaag ccatcaccgg cctgaacgca ctcaaatggg cgcaagagca
     6601 agagcgtttg gtgaaagaca ttattgccga aaccaaagcc caaaccgaaa aagacgtact
     6661 ggcaaaaatc caagcaggcg cggcacacgc caaagcattg gaaaaagaat tggcacgcgc
     6721 caaagccgaa ctcgccgtcc acgcaggcgc caaactcttg gacgatgcaa aagacttggg
     6781 cgcagccaaa ctcgttgccg cccaaatcga agccgacgca gccgccctgc gcgaaatcgt
     6841 taccgattta accggtaaat ccgacaacgc cgtgattctt ttagcggcag taaacgacgg
     6901 caaagtctcc ctgtgcgccg gcgtatccaa accgttgacc ggcaaagtga aagcaggcga
     6961 tctggttaaa tttgcagccg aacaagtcgg cggcaaaggc ggcggcagac cagatttggc
     7021 gcaagccggc ggcacggatg ccgacaaatt gcccgccgtg ttggatagcg tgaaagactg
     7081 ggtcggcgcg aagctggttt gatgtgggaa aggcagcctg aaaggtttca ggctgccttt
     7141 tgtgcaaaga ggccgtctga aaggtctcgt ttgccgtagg ttgggtcgcg acccaacaaa
     7201 ttttgtgaag tataaaaatg ttggtcatga cccaacctac ctgccttttt gtacaaagag
     7261 gctatctgaa aggccttgtt tgccgtatgg tgggtcgcga cccagcagat ttttattagg
     7321 gtatgaccca agctacttgc tacgataaaa aaggattttt aaatgagcat tagccttatt
     7381 ggactacaca ttaccatagc aatcattttg ttttttacta caaattttat gggaaaaaaa
     7441 tcatctatat ttggctatta ccaactgtct tttagcgaag aaaatcactc tccggcattt
     7501 aatatttttt acagagcatt tacccctata ttatttatcg ttattttttc ttgggttgtt
     7561 actagtcttg aaattcccat ttctcttgaa aagataaact atgtagtaat ttattatttt
     7621 ataattagat tgttatctgt atttgttttt gagaaaacac acatagttaa ctggtttaat
     7681 caactaacaa tacccatact atccataaca ttatcattta tagtatataa caaaatgatt
     7741 ttgcccaaaa gttttctact tccatcctca caagaagtag ctactacttt ttgaatagcg
     7801 cttggtggtt acatatataa tatattaaat aatgaatcag ggcatttaaa atcttataaa
     7861 gaaagaagag taaattatgt aaaacacatg cacaaaaaat ttgaaagtta ttttggtaaa
     7921 attatagata aaataatcaa agaggatagt tataataatg atgatttttt aaccgataag
     7981 aaaaaagcac taatatattc agttttaatt tatgagaatt ttaatagggg actagtttat
     8041 agatattttg aaaaaaatta ttttgtactg gtagaataaa aacatttgga ataatgcaag
     8101 taacctcagc agagtacctt tccaatgagg aaagtataaa aaaaggcgga aatattctta
     8161 tggaaaaata caatgaaaaa tataatgaat ctattgatgg caataaaact ctctataaat
     8221 catattatga atcaagaaga gagagtatta aaaactacaa cccagatgca aaatacatta
     8281 atgaaattga atcaatttac atgatgcttg gagaaatcta tccaaatgca ccagacttca
     8341 tgtcaccaca ttttgagggg gactgctctg agggggaata aaatcattat ttattcttta
     8401 ttagttatta gcaggatttg tcgggcataa atgcccgacc tacaaattca attttttcaa
     8461 acctctgcca aatattttca tctttgcaag gctgtctgaa aacccaaacc ccattttcag
     8521 acggcctttt ttcgctaaaa tccccatacc gttcaatccg aaaacacagg agaatcatca
     8581 tggaagttac catctccgcc atcatcaatg gcgaatttgc cgaccaatac ggcaagcgcg
     8641 gtagtcagtt taatgaaaac gggatgctga tttaattcta tttcctttga aactaccaat
     8701 aacctgcctc catcataaaa ctaaaagcaa gccgtagcct gcattcccac aaaccgcgtg
     8761 cgttgccatg tcacacaccc tacctgcggg cgacgcaaac cttaagagac ctttgcaaaa
     8821 ttccccaaaa tcccctaaat tcccac
//
LOCUS       DAWIVB010000068         8831 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWIVB010000068 DAWIVB010000000
VERSION     DAWIVB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.068"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..79)
                     /locus_tag="WIX60_001129"
     CDS             complement(<1..79)
                     /locus_tag="WIX60_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ5305405.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREA"
     gene            complement(222..743)
                     /locus_tag="WIX60_001130"
     CDS             complement(222..743)
                     /locus_tag="WIX60_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305406.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            complement(745..1917)
                     /locus_tag="WIX60_001131"
     CDS             complement(745..1917)
                     /locus_tag="WIX60_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ5305407.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIGTSPASISMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAQREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIVRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDAVKHLLPDSVQALITVIGFNETLE
                     LVRLMGGTTYPLRQGYTKNSQSRVAYLEEIIGSEAAGRLVEAMAPCNLFIPRCETALY
                     ELRNRKIRSQFDRQTAGGTPAYEAVNDLALAHRLSDRHVWRILKQADKEAEQENLF"
     gene            1963..2220
                     /locus_tag="WIX60_001132"
     CDS             1963..2220
                     /locus_tag="WIX60_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ5305408.1"
                     /translation="MKEFPDLEKRLIMLEVAVQDLEDRSLADSFVLSWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLELVESAQELA"
     gene            complement(2287..4272)
                     /locus_tag="WIX60_001133"
     CDS             complement(2287..4272)
                     /locus_tag="WIX60_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ5305409.1"
                     /translation="MKISASDIAKLGIPSLPTDRQGIEYHAKKNNWQHCFEQKGRGRP
                     KKLYEIASLPAEIRAAIMKRQSDELAEKMPKMLPKVRPGTAMSAQALAEAAKLLNEKQ
                     RSVADARCAVVAAVLGIKYEYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRT
                     AKVGERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAP
                     KLAHSYRWFVQWAEAENMPVNDVPNLSMVRRVWEKLPLIMQERGRKTGAAYKSLLPYV
                     KRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAES
                     CVAISDAMRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRPGIRHETGIAGNPQ
                     GRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQ
                     KYLKKLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPD
                     MLSAQELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVNV
                     FDMDGKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQ
                     AAVWDELGHLGGNDIEAEYAVLPKTGTDDFVLFEADR"
     gene            complement(4334..4576)
                     /locus_tag="WIX60_001134"
     CDS             complement(4334..4576)
                     /locus_tag="WIX60_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ5305410.1"
                     /translation="MTDWHRADIVARLKKAGWSVRALSIEANLAPNTLGKALDAPYLK
                     GERIIAAAIGVPAEEIWPSRFEKRNHKPTFPRSINR"
     gene            5086..5526
                     /locus_tag="WIX60_001135"
     CDS             5086..5526
                     /locus_tag="WIX60_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5305411.1"
                     /translation="MGNPVDLSEFVFVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWI
                     DNYVTKQPEKLSVITVKGDSMEGVLNHEDSILINHAETDPRDGLYVLRIGNDLFVKRV
                     QRIPGKLLVTSENPRYAPFEIDLSNAQDDIAIIGRVEWYGRSID"
     gene            5775..6326
                     /locus_tag="WIX60_001136"
     CDS             5775..6326
                     /locus_tag="WIX60_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ5305412.1"
                     /translation="MSGININLKNGDSLLIRGPSGCGKTSLLRALAGLWPFGSSGKVS
                     RPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELAEAMNTCRLGYLVDKLDKTDD
                     WQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPTEALLYRALKQKLPDSIIIS
                     IGHRSTLNAFHNMRLDVGNVACG"
     gene            6431..7099
                     /gene="hda"
                     /locus_tag="WIX60_001137"
     CDS             6431..7099
                     /gene="hda"
                     /locus_tag="WIX60_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ5305413.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            7096..7764
                     /locus_tag="WIX60_001138"
     CDS             7096..7764
                     /locus_tag="WIX60_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ5305414.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            8120..8227
                     /locus_tag="WIX60_001139"
                     /pseudo
     CDS             8120..8227
                     /locus_tag="WIX60_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2261 a         2428 c         1816 g         2326 t
ORIGIN      
        1 ccgcctcacg gtcgctgccg ttgactgcgc ccgggtcggt gttactgtgt ccggcagtca
       61 gacaaataat tttgctcatt aaagcctccc tcaaaatcag attaaaatgc actttcagag
      121 gcttacattt tcaaacggca tggcttttgc agcgggcgaa acagtgtcag taggcaacaa
      181 aaaagccgcc tgttgttcag acggccttag aatcgtttat tttatttttt cggcaaattg
      241 gcttcagctt ccgttattgt tttacggcat tcttgcattg attttcggta attttgataa
      301 ctttccttat ctaaatcagt tgtaccgcct ccaccgcttg ttaccatact cttcatcagg
      361 atttctgcat cttgcgcagc ctgtttgcag acgcggtatg ggtcatcatc tccattcgtg
      421 tccgcataat aagcgactgc ttgagtaata ttttcacgat actttgcaag attttcttga
      481 ttaggtattt ctccttttaa aaacctctca aaatccccgc caaggccttg ttgcaataca
      541 cggaaacggg cggcgcgttc aaaataaaaa gcctgtttct catctgcaaa ttgatattta
      601 ttggcaaaaa aatactgctc cgcatcagct ttctgcgatt gttcttgccc acacgctgcc
      661 aaacccaacg ccagcagcac aaaataaaac tttttcatat ttttctccaa aataaaatgc
      721 catacatctg catggcattc cattctaaaa caaattctcc tgctccgctt ccttatccgc
      781 ctgctttaaa attcgccaca catggcggtc gcttaggcgg tgtgccaagg ccaaatcgtt
      841 aacggcctca taagcagggg taccgcctgc cgtctgccgg tcaaactgac tgcggatttt
      901 acggtttcgc aactcataca aggccgtctc gcaacggggt ataaacagat tgcacggagc
      961 cattgcctcc accagccgac cggccgcctc actgccgata atctcttcca agtatgcaac
     1021 acgggattga ctgtttttcg tataaccctg ccgcaaagga taagtcgtac cgcccatcag
     1081 gcgcaccagc tccagcgttt cattaaaccc gatgaccgta atcaacgctt gtacactatc
     1141 agggagcaga tgcttgaccg catcaggcaa agccgctttc gcaatctcaa agagttcgtc
     1201 atccggcaaa gattcgccca aattcagcgc aacagacacg cggctgtaaa gctgtaccag
     1261 ttcgccatgc ttaccgcgca ggttggccac cagtcggggc ataccgctca acaccaagcc
     1321 gcagccagtc tcgtcgtgca gacggcgtac aatttcaagg gcgcgtaaag gcaggttttc
     1381 cgcttcatcg accacaatca gacggcccga atcgcgcagg cggtcagata cagactcaaa
     1441 caaatcattc aggctgccca tcgccgatac cttcgccgca gtcgccaact tgcgcatcaa
     1501 gaccaaagcc gtaaagctcg gattagcctc aatcaagatg gccgcagggt ttttctcgca
     1561 gtagtttttg accgcctgag tcttgcccaa tcccgcctga ccgtagatca ccactgtttc
     1621 gccgccttcg tgcgcatcgc gcatcacttc tgcaatacgg cgggtcgtct tagtcgatac
     1681 aaaacccaac accagctctt cgcgttgcgc tttactgtcc tgcatctcca aaaacgcctc
     1741 gattttcggc tcgatggttt cataattgcc gcctttttcc gcataagtgc cgttcagata
     1801 catactgatg gatgccggcg aagtaccgat accgcgtgcc agttgggttt ggttcatgcc
     1861 tgatttttct ttaaattcaa ccagtttttg ttgcaatgct tgattaattt gtttcatttt
     1921 taatatcctt gagttttaaa caacctttaa aggccgtctg aaatgaaaga gtttcccgat
     1981 ttagaaaaac gcctgatcat gctggaagtc gccgttcaag acttggaaga caggtcactt
     2041 gccgattctt tcgtactttc ctggctgctc cagcggatta cccgtcaaga gcctacttcg
     2101 attgagcagg ttcgcagttt ccttcaagcg caggcaaaaa cgtttgagcc tgattccgtt
     2161 cagcgggaac accttgaatc tttgcttgag ctcgttgaat ccgcccaaga gctcgcttga
     2221 gtttcaattt ttcaacaagc tcggccgaaa acgctgcatt ttgctgtttg tccatcatgt
     2281 tttcctttat ctatccgcct caaacaacac aaaatcgtct gtgcccgttt tcggcaatac
     2341 cgcatactcc gcctcgatgt cgtttccgcc caaatgtccc agctcgtccc aaaccgcagc
     2401 ctgttccaga gcaggattga cttccgcgtt tgcgagcttg attgcatttt ccgcccgctt
     2461 tattttgcct tttcgacgtt tttccgccag ttggtcgata cgagccgtcg ggaaagcctc
     2521 gcgggtattg ccgttggcct gcgccttagt aataaacttg ccgtccatat caaacacatt
     2581 gaccgccgac gcatcgctca aatcgtagct gacccgtacc tcgtctttgt gatactccgc
     2641 cagctcggtt gagaaataag agttgttaaa caaatccagc caaccgcgct gtacctttcg
     2701 cacctcttgc ggcataaaca tcgtcgccag ctcttgcgcc gacaacatat ccggcgcgat
     2761 accgtcctgt tccagcctca tttcccgata agccttaggc gtataatgcc cgccgtcagg
     2821 atgtcggggc agctcgccgt gcgggcggtt gttgtattcg tcgatacact tgaccacatc
     2881 cgctataaaa cgcgaccagc tcggcagttt tttcaaatat ttctgttgtt cctccgtcaa
     2941 atccttgcct ttttccaaag cgttaaacgc actttccatc ttgcggtaca tcaggttctt
     3001 cgtgctgctg tccatccctg cacccgcaaa cgtctcatac tggcgcgcca tctcaatcag
     3061 attgtctttc caccatcgct caatgatgcc gcgcccttgc gggttgcccg cgatacccgt
     3121 ttcatggcgg atacccggtc gggacgtaat acccgtgatt tcatggtcta tcgtcttgcc
     3181 ggtttggccg ccgccgttat ccgagtaata gataatcggc aaaccaaaat gcttgacccc
     3241 gatacgcata gcgtccgata tcgccacaca actttcagca agagagaccg aaaacccgac
     3301 cacaaaccgc gtacaaccat caataatcac cgtcacttcc ggcttaaatg gtctgccatg
     3361 taccggatgc gccacctttg ccttaaagct atggccgtcg ccgatccaaa catcgttcgg
     3421 cttcaaagcc ccccaatcac gtttcacata aggcagcagc gatttataag ccgcccccgt
     3481 tttcctgccg cgctcctgca taatcaacgg gagcttttcc caaacgcgcc gcaccatact
     3541 caagttaggc acatcattga ccggcatatt ttccgcttcc gcccactgca caaaccagcg
     3601 gtagctgtgc gccagctttg gcgcggacgg aatattgtga aactgcataa acatcggcaa
     3661 ccatccgtaa ctttcaatcg gcttgaccgc cttcgtcgtc ttcggagcca aagcaaccaa
     3721 ccgctccgtc gcgttttccg ctttcaaata agcagaaatc cagccgtcta aagtacgttc
     3781 gccgaccttc gccgtccggc tgcggtcatt ggccttttcc aagttcccaa gcgtgaccgc
     3841 gtccaattta ccttctgcca gcaagcccaa aaactgagcc accgcagcct tggcagagca
     3901 atcgtattcg tatttaatcc ccaataccgc cgctaccacc gcacatcgcg catccgccac
     3961 cgaccgttgt ttctcgttca acagcttggc cgcttcagcc agtgcttgag ccgacatcgc
     4021 cgtccccggt ctgactttgg gcagcatttt cggcatcttc tccgccagct cgtccgactg
     4081 ccgtttcatg atggctgctc ggatttcggc agggagggaa gcgatttcat acagtttttt
     4141 aggacggcct cttccttttt gctcaaaaca gtgttgccaa ttatttttct tggcatggta
     4201 ttcaatccct tgtctatcag ttggtaggct cggaattcct aatttcgcaa tatcagatgc
     4261 agatattttc attttttccg ctttctattt ttgcttaaat gcggtactct tttggaacta
     4321 tttagcaaaa cagttatcta tttatagatc ttgggaaggt tggcttatgg tttcgtttct
     4381 caaaacgaga tggccagatt tcttctgcgg gtactccaat cgctgctgca atgattcttt
     4441 cgcctttcag ataaggagca tctaaagctt tccctaatgt atttggtgct aaattagcct
     4501 cgatagataa cgctctcaca gaccagcctg cctttttaag acgagccaca atgtcagcac
     4561 gatgccaatc agtcatgctt tcttgctttt tttcattctc gcaattcctt aattaagtag
     4621 gtttattaac cgccgtcatt tagcaaatga gtgaattaca tttagcaaaa cagcttcatg
     4681 aaactctttt gctaaaatat tttctcaaaa acgtaattat ttttacaaaa tattgattat
     4741 ttgagtaatc ttatttaagc aaaagagttt ctttttttgc ttaatgtttt gcttaaatga
     4801 gttaaattag gattactatg gatacttttt tagataggct caaatttctt tggcctcatg
     4861 gggctaagcc atctgacatc tataacaaaa tagaaatgtc ggcttctgga tttaatagag
     4921 tttggaaaga gggagctatc ccaacagccg attacctagt aaaaattcag ttgtgattta
     4981 aactggctgc tgaccggaaa aggcttgccg tatctcgata aagccaggcc ggaaaacgcc
     5041 ggagcattct ctgtaagtag aaattctgat ggaatcaccg acactatggg caatcctgtc
     5101 gacctgtccg agtttgtatt cgtgccgcgc tacaacgtag ccgcagcagc agggtatggc
     5161 gcaccagtct tcggcgaaga acctttattc tgcttggctt tccggaaata ttggattgac
     5221 aactatgtga ccaaacagcc ggaaaaactg tctgtaatta ccgttaaagg cgattcaatg
     5281 gagggcgtgc tcaatcatga ggattccatt ctcatcaacc acgccgaaac tgacccgcgt
     5341 gacggcctat atgtcttgcg tataggcaat gacctctttg tcaaacgtgt gcaacgtata
     5401 ccgggcaaac tgcttgtaac gtctgaaaat ccacgttacg caccgtttga aatagatttg
     5461 agcaatgccc aagacgacat cgccatcata ggccgtgttg aatggtatgg ccggagtatt
     5521 gattaaatcc attttaaaaa cctcttaaaa cagttttaaa aattccctaa tctcgtcagc
     5581 tttaaacaga aaaccgcgca ttccggcgcg gttttgtgaa aaagctggcg taacttttcc
     5641 ggatacaaaa aaacgccgaa atccacgtct tccgaagatt tcagcgtttt tttactctat
     5701 ttgttccttg tgcaaaaact aacagtcccc cacaggaaaa cgtcgctctg ttccgccaca
     5761 acggcgaaat cctgttgagc ggcatcaaca taaacctcaa aaacggcgat tccctgctga
     5821 tacgcggccc gagcggttgc ggcaaaacct cgctgttgcg cgcgctggcg gggctttggc
     5881 cgttcggcag cagcggcaaa gtcagccgtc cgccgcatca agacatcctc ttcctgccgc
     5941 aacgcccgta cacggcacag ggcagcctgc gcgacgcggt ttgttacccc gacattgaca
     6001 agcagcatcc cgagttagcc gaagccatga acacctgccg cttgggttat ctggttgata
     6061 aattagataa aaccgacgac tggcaacaca aactctcccc gggcgaactg caacgcgtcg
     6121 ccttcgtccg cgccctgctt tccaagccca aaatcgtcct gctcgacgaa gccaccgccg
     6181 ctttggacga accgaccgag gccctgctct accgcgcact gaaacaaaaa ctgcccgaca
     6241 gcatcatcat cagcatcggc caccgcagca cgctcaacgc gttccacaat atgcgcctcg
     6301 atgtcggcaa cgttgcctgc ggttgaacga tgccgtctga agccgttttc agacggcatc
     6361 gttcaaattg gaaaggcgcg cgaaaacaac gtacaataaa cccgtttacc acccctcgga
     6421 atgccgaacc gtgaaccagc ttattttcga ctttgccgca cacgactatc cgagtttcga
     6481 caaattcctc ggcacggaaa acgcggaact ggtctatgtc ctccgacaca aacacggaca
     6541 gtttatctat gtctggggtg aagaaggcgc gggcaaaagc catcttttgc aggcgtgggt
     6601 cgcacaggcg ctcgaagccg gcaaaaacgc cgcctatatc gatgccgcct ccatgccgct
     6661 gaccgatgcc gcgttcgagg cggaatacct cgccgtcgat caagtcgaaa aactgggcaa
     6721 cgaagaacaa gccctgcttt tttccatctt caaccgcttc cgcaacagcg gcaaaggctt
     6781 tttgctgctc ggttcggaat acacgcccca gcagcttgtc atccgcgaag atttgcggac
     6841 gcgtatggcg tactgcctcg tttacgaagt caaaccctta accgaccaag aaaaaatcga
     6901 cgcgctcgcc agtatggcgg cggcacgcca agtaaccgtc gattccgaaa ttttcgaata
     6961 cctgctcaaa cactggcggc gcgatatgga cagcctgatg atgatgctcg acacgctgga
     7021 caactacgcc gtcacgatgg gcaaacgcat caccctgccg cttttgcgcc agcttttgaa
     7081 acaacaggaa acccaatgaa aaacctcgcc atcttcgacc tcgacaacac cctcatcaac
     7141 accgattccg accacgcctg gccgcaatac ctcatcaaaa aaggcttggt tgacgctgcc
     7201 gaaaccgaag cgcaaaacga aaaattctac cgcgactacc aaaacggctg cctcgacatc
     7261 gacgcattcc tcaaattcca ccttgccccg ctcgctcgtt acagcaaaga agagctggca
     7321 gagtttcacc gcgaatttat ggcggaatac atcatccccc acatctcgcc tatgcagcgt
     7381 atgctggtgc agagccacca aatggcaggc gacgaaaccc ttgtgatttc ctcaaccaac
     7441 gagttcatca tcacccccgt ctgccacctt ttcggcatca ccaacatcat cggcacacaa
     7501 ctcgaaaccg gttctgacgg ccgctacacc ggcaattaca tcggcacgcc cagcctcaaa
     7561 gaaggcaaaa tcacccgcct gaaccaatgg cttgccgaac gcggcgaaac gctccaaagc
     7621 tacggcaaaa cctattttta cagcgactcc aaaaacgacc tgccgctgct gcgccttgtc
     7681 agcgaacccg tcgccgtcaa ccccgatgcc gagctggaaa aagaagccaa agaaaaaggc
     7741 tggccggttt tgaatttcaa atgacgcaat gccgtctgaa gcccgattcg gcgttcagac
     7801 ggcattgagg ctgaaatcca aaaaactcaa agccacagga atttatccgg aaaaacaaaa
     7861 aacacaggaa ttcatcggga aaaacaacaa tctttccacc gtcattccca cgaaagtggg
     7921 aatcttgatc cgtccgcacg gaaacttata tttcgtcatt tgcacccgac ataattcaat
     7981 actttatttt gcaataaatt aacggatgct gacaaattag attggattaa ttaccctatt
     8041 atccatattg ggttatcggg ttgctaattg agcagagcgt agcaaaccat aaagtgagtg
     8101 tttccaccca ctctgcattt atagtggatt aaatttaaat caggacaagg cgacaaagcc
     8161 gcagacagta cagatagtac ggcaaggcga ggcaacgctg tactggttta aatttaatcc
     8221 actatactat gaagtggtta ttaaatatga taatgagacc tattaaattt agtatggtaa
     8281 atacgttatt atttattgtt atatgtagtt cattttttga tctgctcgtt caattatgta
     8341 caattttatt tcatagccaa aaaatatact ttattacatt atttttatta tttattttta
     8401 attttgttac aaaatctatc tatatggcaa ttatttatcc tattttatat ttttttacga
     8461 taaaaaaata ttatccttac tctaggaaag tgataattct attatcatta gcattatcta
     8521 tatattttag ttttatggac ttttactttt tttccatata ttcagataac cttagctatg
     8581 aaacggagcc tttacattta tacatcccta ttattattaa ttttttctca cttttagttt
     8641 ctaattttat tttatctttt atcaacaagt aaatgtatat acaaaataca atccaaaaga
     8701 tcctaagcaa accaccaatc ccacaccacc cttaccggtt caaatccgca caaaaaccca
     8761 catcccgtca ttcccacgaa agtgggaatc taggacgtat agtggattaa caaaaatcag
     8821 gacaaggcga c
//
LOCUS       DAWIVB010000069         8815 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWIVB010000069 DAWIVB010000000
VERSION     DAWIVB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.069"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            179..1354
                     /locus_tag="WIX60_001140"
     CDS             179..1354
                     /locus_tag="WIX60_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ5305415.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(1565..2113)
                     /locus_tag="WIX60_001141"
     CDS             complement(1565..2113)
                     /locus_tag="WIX60_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ5305416.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WIX60_001142"
     CDS             complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WIX60_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ5305417.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            4140..6119
                     /gene="tkt"
                     /locus_tag="WIX60_001143"
     CDS             4140..6119
                     /gene="tkt"
                     /locus_tag="WIX60_001143"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ5305418.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPEGLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            6268..7656
                     /gene="fumC"
                     /locus_tag="WIX60_001144"
     CDS             6268..7656
                     /gene="fumC"
                     /locus_tag="WIX60_001144"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ5305419.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLSGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIHLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(7721..8644)
                     /locus_tag="WIX60_001145"
     CDS             complement(7721..8644)
                     /locus_tag="WIX60_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ5305420.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALIVVGGAVTAAVGDRLFKRR"
BASE COUNT         2227 a         2519 c         2157 g         1912 t
ORIGIN      
        1 cactataata ttttctctcc cgattgaaac aggcgtaaca gaatatgccc gaagctccgg
       61 ctgctttctt gtttaccgcc gcgatattta gagtataata ccaaatttga gcaatagttc
      121 taaaacagtt agaaccattt ttcatgagcc tgactgattc gtacactcgg agaaactgat
      181 gcagaatatt tttgaccctt tggttattcg tggaaaatcc cttaccccca tcgtgcaagg
      241 cggtatgggg gtcggtgttt ccgcatcggg tttatccagc gcggtggcgc gtgaaaacgg
      301 tatcggaacg attgccagtg tggatttgcg ccaccttcac gaagacctac tcgccgaatc
      361 acaaatcaat ccgagtgaag agaaatatac atctttgaac tgtaccgcat tagacaggga
      421 aatccaaaaa gccaaaagcg cttcagaggg aaaaggactg attgcggtca acgtgatgaa
      481 ggcggtcaaa gaccacgccg catatgtccg ccaggcttgc gaatcagggg cggatgcggt
      541 tgtaatgggt gccggcctgc ctttagacct gccggaaatg accgagggct atcataaaga
      601 tgtcgcgctg ctgccgattc tgtccgaatc gcgcggtatt aatatcgtct tgaaacgttg
      661 gatgaaaaaa ggcatattgc ccgatgcgat tgtagtcgaa catcctgccc acgcggccgg
      721 acatttgggt gcatcaaccg ttgaaggcgt aaacgatgcc aagttcgact tcaaacgcgt
      781 gattgaggaa acgtttgaag ttttcaaaag tttagggctg gaaagcgaaa aaatcccgct
      841 tattcttgcg ggaggcatgg caaattttga aaaagtcaaa accgccctaa agaactgggg
      901 agcatccgcc gttcaaatcg gtacggcttt tgccgttacc gaagaaggag atgcacacct
      961 taacttcaaa aaaacgctcg ccggtgcgga aactgaaaaa gtagtcgaat ttatgtctgt
     1021 tgccggtttg ccggcgcgcg gtgtccgcac caaattccta gacagctaca tcaagcgtga
     1081 aagcaaactt cagacaaacg ccaaagccga cccgcgccgc tgtacccaag gtttaaactg
     1141 cctaaccagt tgcggtctgc gcgacgggct ttccaaagca ggacagttct gtattgatat
     1201 ccagcttgcc gccgcattcc gtggagaagt agataaaggc ctgttcttca gaggtaaaga
     1261 cccgctgccc ttcggcaatg ccatccgcac cgtccgcgag acgatacaat atctgctgac
     1321 ggggagcgaa cctgttgcaa cgctcggacg ctgacatcag gtaggatttg atttgcaaac
     1381 aaaatgccgt ctgaagggct ttcagacggc gtttttcagg ctgcgttccg gaatagtgtt
     1441 aaaaaataaa cgggatgaga tacatttatt ttcgtccgac aaatcaaacc atcgcgccga
     1501 atgatcaaat aatgcctgca cggcattaca tctggcaaag caatgcaatg aaaacacggc
     1561 ttttttattt gctttcagta ttattgaaaa gcttgtccat cggggtcaaa tcgaccgcat
     1621 tgccttggct ggtaatccat ttgttctcga cgcgccacac gcctgccgcg tcctccacgc
     1681 gcacatacca gtggttggtc ggcgaaaggg ttttgaacac cgcctcatat tccgccctgc
     1741 cgttctgcgc gctgccgacg ggcttgaggg cgacggtttg atcgtccgcc ttgcgggtcg
     1801 ggtgcatcag cagcaggttc aaaggctgtt tgccgtcaaa ctcgccgccg acaaacactt
     1861 ttgccgcatt catatcgggg gaaatgagaa cctgcacccc gatatgccgt ctgacggctt
     1921 cttcatcccg atgaagctgg atgtcgatat gtttgccgtc tttataataa tcgtccgtaa
     1981 ccaaatctgt cgcgtgctgc tgcgcgacaa aaaacatagc gacgctggcg atgacgacaa
     2041 aaatcggccc cgccatcaag atccacggcc agacgtgttt gtaccaaggt ttgattggag
     2101 tgttttgaga cacggttatt ctccgataaa ggttgcatct tcttccaaga cgaccggctt
     2161 gccgtcgggc gcgccgcttt cgcggtattg gaaggtaaat tcgatagggt ggctgccttt
     2221 gtccgcgtat tccggaatgg tggacacttg gacgggaagg gttaccgttt cgcgcggggc
     2281 aaccttgata ccgccttcgg gcagcccggt cagggcgatt tcgtcaaagc ctttgacact
     2341 tgcggtaatc agctgttctt tttcactttt gttgatgata cgcaggctgt atgcgttttc
     2401 cagccagcct ttggcgtttt cgcgcaccag tacgccacgg tctttcaaaa tatcgacctc
     2461 gaccattttg cgcgtggaca aaccggccag gaaggcaatg ataactaacg ccaacaccgc
     2521 gccgtaacct gccacgcgcg gtctgagcag ccgtttttta atgtcttttt cagaatattc
     2581 gtgttccagc gcgctttcgg tcgtataacg gattaatccg cgcggatagc ccattttgtc
     2641 cataatctca tcgcacgcgt cgatacaggc ggcgcagccg atacattggt attgcagacc
     2701 gttgcggatg tcgatgccga cggggcagac ttggacgcac atcgcacagt tgatgcagtc
     2761 gcccaaaccc gcctcttcct tattgaccgt tttcttgcgc gcgccgcgcg gttcgccgcg
     2821 ttccgcgtca taagaaacaa tcagcgtgtc cttgtcgaac atcgcgcttt ggaaacgtgc
     2881 atacggacac atatgcaggc atactttttc acgcataatg tgggcgaaga agaaggtcat
     2941 aaagccataa aacgctgcgg caaacatcgc gccgccacct gctgctccag tgaataaatc
     3001 gggaacgaac tggcggatag ggacaaacca gcctgcaaac gtgatgcccg tccacgcgca
     3061 gacaaggaaa atcagcaggt atttggtggc tttgatgcgg attttagtga aattccacgg
     3121 cgatttttcc agtttcagcc gtttgtttct atcgccttcg accaggttgt caatccacag
     3181 cataatttcg gtgtaaaccg tttgcgggca ggaatagccg caccacagtc gccctgcaat
     3241 cgtcgtccac caaaacagcc cgaaggcgca aatcatcagc agcaaggcaa ggtaaatcaa
     3301 atcgcccacc cccaacgaca atccgaaaat gaagaaatgc cgttcgggga tattgaaaac
     3361 gacggcctgc ctgccgctcc agttgaacca cggaatgacg taaaacacaa actgcgtcgc
     3421 caatacggcg gcgatacgca gtttggcgaa ccgtccttcc gcctttttgg gatggatgcg
     3481 ttcgccttcg ggatggattt gaatcacgct ggctcgcgga tcgaatgttt tttttgcttt
     3541 cggcgcggct tttgtttgtt cggacgtgcc gattccggat gccggactgc cggcttggtt
     3601 ttccgtggtc attctgcatt ccttagattt ttgattgatg gtttgcccgt taccgccgcc
     3661 gtttgctttt cagacgtcat ttttcttgtt ttttaaggcg ttgtgtttca agttttgaga
     3721 aaatccgttt ttcccaaaat atatttccgc tattgtacaa ctttatgcgc cgtccggatg
     3781 tatggggcgg atacatttcc catccgcatc aaaacgcctg gattttacct taccgcccga
     3841 acaaaatccg aatacggtta aaaaaaaaga ctaaaaaaac cgacaccccc atatcggcag
     3901 aaccgacggc gcaagctcat aaacaaacgc tatcgacaat ccggcacaca atctataact
     3961 ttttatttca aaaggaataa tggcaggctt cgcccgcaaa tcgaaaatcc ttccccgcct
     4021 gtcccctgcc gccgccttcc cacgcgtccg cccttttctt gaaagcataa gcgaatcggg
     4081 cgataatcaa cgctttccga ttatccactt atctgaaaca ccagcaagga aaatacaaaa
     4141 tgtctcaact ggcaaacgca atccgcttcc tctcggccga tgccgttcaa aaagccaatt
     4201 ccggccaccc cggcgcgcct atgggtatgg cggaaatggc ggaaacattg tggacgaaat
     4261 tcctcaatca caaccccgcc aaccccaaat tctacaaccg cgaccgcttc gtcctctcca
     4321 acggccacgc gtctatgctg ttgtacagcc tgctgcacct gaccggctac aacctaagca
     4381 ttgaagactt gaaaaacttc cgccaactgc acagcaaaac ccccggccat cccgaatacg
     4441 gctacaccga cggcgtggaa accacgaccg gcccgttggg gcaagggatt gccaacgcgg
     4501 tgggtatggc attggcagaa aaaatccttg ccgccgaatt taataaagac ggtttgaaca
     4561 tcgtcgatca ttacacctac gtctttatgg gcgacggctg tctgatggaa ggcgtatcgc
     4621 acgaagcctg ttcgctcgcc ggcaccttgg gcttgggcaa actgattgtt ttatatgatg
     4681 acaacaatat ttccattgat ggtaaagtgg acggctggtt taccgaaaac atcccgcaac
     4741 gctttgaaag ctacggctgg cacgtcgttc ccaatgtaaa cggtcatgac accgccgcca
     4801 ttcaagccgc catcgaagcc gcacgtgccg aaaccggcaa accgtccatc atctgctgca
     4861 aaaccttaat cggcaaaggc agtgccaaca aagaaggcag ccacaaaacc cacggcgcac
     4921 ctttgggcgc ggacgaaatc gaagccacgc gcaaacattt gggctggact taccccgcct
     4981 ttgaaatccc gcaagaaatt tacgatgcgt ggaatgccaa agaacaaggc gcgaaactgg
     5041 aagccgactg gaacgaactg ttcgcgcaat atcaagccaa atatcctgcc gaagccgcag
     5101 aatttgtgcg ccgtatggat aaaaagctgc cggacaattt cgatgaatac gttcaagccg
     5161 cattgaaaga agtgtgcgcc aaagccgaaa ccatcgccac ccgcaaagcc agccaaaaca
     5221 gcatcgaaat cttggcaaaa gagttgcctg aattggtagg cggttctgcc gacctgaccc
     5281 cgtccaatct gaccgactgg tcaaacagcg tctccgttac ccgcgacaaa ggcggcaact
     5341 acatccacta cggcgtgcgc gagttcggca tgggtgcgat tatgaacggt ttggtattgc
     5401 acggcggcgt aaaacccttc ggcgcgactt tcctgatgtt cagcgaatac gagcgcaatg
     5461 ccctgcgtat ggctgcgttg atgaaaatca accctgtatt tgtgtttacc cacgattcca
     5521 tcggtttggg cgaagacggc ccgacccatc aaccgattga gcaaaccgcc accctgcgcc
     5581 tgattccgaa tatggacgta tggcggccgt gcgacaccgc cgaatccttg gtggcttggg
     5641 cagaagccgt caaagccgcc gatcacccgt cctgcctgat tttcagccgt caaaacctga
     5701 aattccaagc gcgcagcgag caacaactga acgacatcaa acgcggcggc tacgtcatca
     5761 gcgaagccca aggcaacgcc caagccgtca tcattgccac cggctcagaa gtcgagctgg
     5821 ctttggaagc gcaaaaagcc ctcgccgcgc aaaacatcgc cgtgcgcgtc gtttccatgc
     5881 cgtccaccaa cgtattcgac cgccaagacg ccgcctatca agccgccgtc ctgcccgaag
     5941 gcctgccgcg catcgccgta gaagccggac acgccgacgg ctggtacaaa tatgtcggac
     6001 tgaacggcgc agtcgtcggc atcaaccgct tcggcgaatc cgcccctgcc gatttactct
     6061 tcaaagcatt cggctttacc gtggacaatg tggttgatac ggtgaaatcc gtgctgtaac
     6121 cccacaccta aacaaatgcc gtctgaaacc aattagggct tcagacggca tttttatatt
     6181 ctcgcggcca tgatgctttc tcatcccacc aatctccatt ataatatttg cgaatcactc
     6241 ttattcacat ttcaaaagga gaaacgcatg agcacccgta ccgaacacga cacgatgggc
     6301 aatgtcgaag tcccatccga agcctattgg ggcgcgcaga cccagcgcag ccgcaacaat
     6361 ttcaaaatcg gtggcgaaac cctgccgcag ccgttgattt atgctttggc attggtgaaa
     6421 aaagccgccg ctgccaccaa tgtttccctc ggtaggatta agcctgaaca ggcggatttg
     6481 attacgcagg cggcggatga tgtgttgagc ggcaagctcg acgggcagtt cccattggta
     6541 gtgtggcaga ccggttccgg cacgcagtcc aatatgaaca tgaacgaagt gctggcaaac
     6601 cgcgccaacg aaatcgccgg tacgggtttg gcggcttatc agcccgtcca tcccaacgac
     6661 catgtgaacc acgcgcaatc gaccaacgac gcattcccga ccgctatcca cgttgccgcc
     6721 gcgattgaaa tcaaccgcca cctcatcccc gccgtaaaag ccctgcgcga cacgttggac
     6781 aaaaaagccc aagctttcgc ccctatcgtc aaaatcggcc gcacccactt gcaagacgcg
     6841 acgccgctga ctttgggaca ggaattttcc ggctacgttt cccagcttga tcacggttta
     6901 ggccgtctga acgatgcgct taaagacttg tatgaacttg ctttgggcgg tacggcggtc
     6961 ggcacgggtt tgaacagcca tcccgaatac gccgaaaaag ccgccgccaa actcgccgaa
     7021 ttgtccggct tgccgtttgt cagcgcgccg aacaaatttg aagccctggg cggacgcgat
     7081 gccgccgttg ccgcttcggg cgcattgaaa acgctggcgg caagcctgaa caaaattgcc
     7141 aacgacatcc gttggctggc aagcggcccg cgttgcggtt tgggcgaaat caaaatcccc
     7201 gaaaacgagc cgggttcgtc cattatgccg ggcaaagtca acccgaccca atgcgaagca
     7261 atgacgatgg tgtgctgcca agtgttcggc aacgacgtta ccatcggtat ggcgggcgcg
     7321 tcgggcaatt tcgagctgaa cgtctatatg cccgttatcg cctacaacct cttgcaatcc
     7381 atccacctgt tgggcgacgc gtgcaacagc ttcaacgaac actgcgccat cggcatcgaa
     7441 cccgtgccgg aaaaaatcga ctatttcctg caccattccc tgatgctggt taccgcatta
     7501 aaccgtaaaa tcggttacga aaacgccgcc aaagtcgcca aaaccgccta caaaaacaac
     7561 aaatcgttgc gcgaaaccgc cgttgagttg ggcttgctga cgggcgaaga atttgacgaa
     7621 ctggtcgttc ctgccgatat ggttcatccg cgctaatcct tccctcaaat aaaatgccgt
     7681 ctgaaacctc gttcggacgg cattttccgt tgcctgcaaa ctagcggcgt ttgaacagcc
     7741 tgtcccccac cgccgccgta accgcacccc cgaccacgat cagtgcgcct gcataaccca
     7801 aaccgttcat atccggcgcg gcaaaagtat caggcatcac ataatgcccg agcaaagaaa
     7861 atattacggt aaacacgggg agcaaggttg ttaccgcgct gactttggaa gcctcccaat
     7921 gtttcaacgc ctcgccgaac gagccgtaac cgattaacgt attcaagcag caatacgcaa
     7981 aacaaaccca cgccaacgta ccgtccaaac ttccgatgtg tgccggttcg gcaaacggca
     8041 ggaacacggc ggcacttgcc gcataaatca acagcagaat ctgttgcggc ccgaattgcg
     8101 ccgacagcag cttttgcgcc acggcataac acacccatgc catactgcct gccgcacaca
     8161 gcaacacgcc cttcgcatac gcgcccaaac ccgacaactc gccgaattta tcgttaaaaa
     8221 acataagcaa accggcaagc agcaaaacca agccgatttt ctgagcggca gtcatccggt
     8281 ctttaaacac caacacaccg acaacaatca tcgtaaacgg cgaaatctgc cacaaaacct
     8341 gcgtcgtggt cggcgaaata taatgcagcc cttgggcaat cagcacaaag tttgccgaaa
     8401 tgcccgccac gccgagcagc agcagcctga atgagcacca agaaaaatcc cgccgcttcg
     8461 gcagccgccc gcccagtgcc agcaaaacaa acaataccgc cgccgccacg gtaaaacgca
     8521 cccacaccag cgtcggcgca tcgacaaact tcaatacctg ccgcacggca atcggcagcg
     8581 ttccccacgt catcgccgcc aaaagtgcca acgcgaagcc taggagcggc ctttggtttt
     8641 ccatcctgat tttcctattt ttaaacaacc gtattgccgg acgatgccgg tttgccgcat
     8701 cgggcaatga tggttcaagc gtttggcgtt tgattccaac cctttgattt caaacaaacc
     8761 ggctgaagct cggctattgc ttcgcgctat ttgaaaacac cgcctgaatt ttaaa
//
LOCUS       DAWIVB010000070         8815 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWIVB010000070 DAWIVB010000000
VERSION     DAWIVB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.070"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(428..1507)
                     /gene="apbC"
                     /locus_tag="WIX60_001146"
     CDS             complement(428..1507)
                     /gene="apbC"
                     /locus_tag="WIX60_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ5305421.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQHSDGIHIALHF
                     GFPVAHIAAAIADSVQETLMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSGGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGAAAQLFDKHPAIARIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
     gene            1718..1793
                     /locus_tag="WIX60_001147"
     tRNA            1718..1793
                     /locus_tag="WIX60_001147"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1751..1753,aa:Lys,seq:ttt)
     gene            1872..2381
                     /locus_tag="WIX60_001148"
     CDS             1872..2381
                     /locus_tag="WIX60_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ5305422.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            2724..3164
                     /gene="nadR"
                     /locus_tag="WIX60_001149"
     CDS             2724..3164
                     /gene="nadR"
                     /locus_tag="WIX60_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ5305423.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3279..3779
                     /gene="hpaC"
                     /locus_tag="WIX60_001150"
     CDS             3279..3779
                     /gene="hpaC"
                     /locus_tag="WIX60_001150"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ5305424.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            3795..4490
                     /locus_tag="WIX60_001151"
     CDS             3795..4490
                     /locus_tag="WIX60_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ5305425.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4721..5152)
                     /locus_tag="WIX60_001152"
     CDS             complement(4721..5152)
                     /locus_tag="WIX60_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ5305426.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGKNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            5327..7003
                     /locus_tag="WIX60_001153"
     CDS             5327..7003
                     /locus_tag="WIX60_001153"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ5305427.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPRKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RVIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            7117..8208
                     /gene="ychF"
                     /locus_tag="WIX60_001154"
     CDS             7117..8208
                     /gene="ychF"
                     /locus_tag="WIX60_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ5305428.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            8534..8638
                     /locus_tag="WIX60_001155"
                     /pseudo
     CDS             8534..8638
                     /locus_tag="WIX60_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8649..>8815
                     /locus_tag="WIX60_001156"
     CDS             8649..>8815
                     /locus_tag="WIX60_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ5305429.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTMILHR"
BASE COUNT         1963 a         2273 c         2535 g         2044 t
ORIGIN      
        1 ctttcccaac aactcaaccg ccccaataca agcctttcgg cttgttgccc tctctccagc
       61 cctctcccac ggggagagag gacggggagg ctgttggggt taagggtttt gtaaactaat
      121 caggttgtgc aggctgcttt ttgcttttca gacgacctgc atcaacggtt gtaaatccaa
      181 tctgtttttc aaaccgcctg caaccgcaaa acctgccgca atccgctccc tcccctgtgg
      241 gggagggccg gggagagggc attctccaag ttgcggcaat ctttcccaac agcttaaccg
      301 ccccaataca agccttgcgg cttgttgccc tctctccagc cctctcccac ggggagagag
      361 gactatgaag cccgccggca ggcgcggggc ttgggacggc attgctgttg cggtttcgga
      421 cgcggcttta ttcgacgaca attttaggga accggctgct gaagtctttg cctttgtcgg
      481 caatgctcag ggcgatttgg aatgcggcat cggtgtagat tcgggcgatg gcggggtgct
      541 tgtcgaacag ttgtgccgcc gcccctccgt ccatggcttc gcgcacgggc aggcttaggg
      601 gaagctgtcc gagcaggggg acgttgaggc gtgccgccaa atctttgccg ccgtccgtgc
      661 cgaacagtgc ttcgctatgc ccgcagttgg agcagatgtg gacggacata ttttccaata
      721 cgcccaaaat gggaatgttg actttgcgga acatatccac ggctttgcgc gcgtctatca
      781 gggcgatgtc ctgcggcgtg gttacgatga cggacccggt aacggggatg cgctgggaca
      841 gcgtgagttg gatgtcgccc gtgccggggg gcaggtcgat gaaaagatag tccacttcgt
      901 cccactcgct ttggaacatc agttgctgca aggcttggct gaccatcggc ccgcgccaga
      961 cgacggcttg gccggtatcg actagaaagc cgatggacat aacctgtatg ccgcctgaag
     1021 attcgacggg aatgagtttt tggtttttct gatcgggttt gcggtcgtcc acacccaaca
     1081 tggtcggttg gctcgggccg taaaggtcgg catcgagcac gccgacgcgc gcgcccatgc
     1141 gcgccattgc ggcggcaagg ttggcggtgg ttgttgattt gcccacgccg ccttttcccg
     1201 atgcgacggc gatgatgttt ttcacgcctt tgatggtggt aacgccgggc tggactttgt
     1261 gtgtgccgat ttcagtgtcc atggacagat ggatgtgtgt gtcgcctgtt tcgggcatca
     1321 gggtttcttg tacggaatcg gcgatggcgg cggcgatgtg cgcgacaggg aagccgaaat
     1381 gcagggcgat atggatgccg tctgaatgct gttcgaccga acggacggcc ttttcgccgc
     1441 cgagcgtgcg tgccgtattc ggaacggcga cggtgtcgag gagggtgcgg atgttttgta
     1501 tgttcataat gtgtgtttct gtgtgcagtt tgggaaacgg gcggaaagtg tacattgatt
     1561 tcgggcggtt tgcttttcct tgtgcgaact tttacacggt atgcggcgga ttgtgaaaat
     1621 ccgttgtcaa aatatcccgt gcaaacagtg gtctgcaatt aagcctgtaa aatttgcttg
     1681 acgaggtagg atggtgctgt ataatgcacg gcttatcggg tcgttagctc agtcggtaga
     1741 gcagcggact tttaatccgt tggtcgagcg ttcgaatcgc tcacgaccca ccagataacc
     1801 ggaagccaag ttttgagact tggctttttg tttgcctgcc ggttgcgcgg gcgtaccgtt
     1861 tgagagagcg tatgaaaaag aaactgcttt cgggcatcaa atttgccgtt cagacggcat
     1921 tggtgttttt attggtgtcg ctgtttttgg attgggtacg caagcccgaa gagccggccg
     1981 gtgcggcagg gcggcctttg accctgctgt cggggcagcg gctgactttg ggacagttta
     2041 gccgggataa ggcggttttg gtgtattttt gggggagctg gtgcggcgtg tgccgttatc
     2101 agtcgccgat aatcgatgat ttggcggcgg acggcgtgcc ggtcgtcggc gtggcggtac
     2161 gttccggcag cgcgtccgaa gtggcggcgt atatggcaaa gcggggcttg ggctttccga
     2221 cggtcaacga tgaggacggg ggtttggcaa ggtcttggcg gattgtcgca acgcctgccg
     2281 tcgttttggt caaaaatggg aaaatggttc actatacgac gggaatcagc agttattggg
     2341 gcttgcgcgc acgaattttt caggcggatg ttttcggtta acttttgttt ttgttaaact
     2401 ttacgctatg tagagaatca aacggcatat accttgctcg tctgatgcgg cggcttttgt
     2461 tttttctgga agaaagttct ttcgacagta aatgtaatac tttgttgaga ttgtagggaa
     2521 aatagactaa gatataacta ttaaaatatt tttagatagg attatcggaa ttaaagtctt
     2581 ttatacccag tcgtccgatg cggtttatag cgtattgttg ctatatgttc gttttgttat
     2641 ataacggttg catcaaaatt tacgcccaca ggctttcccg acggtttgaa agtttgattt
     2701 tcgataactt ggagacttga acaatgccta cccaatcaaa acatgcgtct atcaatatca
     2761 gtctgataca ggcaagggaa gccctgatga cccaattcag gcctattctg aatcaggcga
     2821 atattaccga tcagcaatgg cggattatcc gtcttttggc ggaaaacggc acgctggact
     2881 ttcaagattt ggcgaatcag gcgtgtattc tgcgccccag cctgaccggt atcctgaccc
     2941 gccttgaaaa agcgggtttg gttgtccgcc tgaaaccttc caacgaccaa cgccgcgttt
     3001 ttctgaagct gactgccgag ggcgagaagc tgtatgagga aatcggcgaa gaagtggacg
     3061 agcgttacga cgctatcgag gaagtgctgg gccgcgagaa aatgctgctg cttaaagacc
     3121 tgttggcaga acttgccaaa atcgaggatg cgttgaactc gtaatacgcc gtaacgcgcg
     3181 gaaacgtccg accgacggct ttttgaatca aaactgctgc acatggggga tgccttgtgt
     3241 gcagcattct tatatagggg acagtttaaa ggggaaaaat ggcggatttg cagaaaactt
     3301 ttcaaacttc gttccgtgat gcgatggcat cttgtgcggc aggcgttcat gtcatcacga
     3361 cagacggtgc ggcagggcgt tacggcatta caatgacggc ggttacaccg gttacggacg
     3421 agccgccgac cgtgatgctg tgcatcaacc ggagtgcgcg aatcattccg atcctgtcgg
     3481 aaaacggcag cctctgcatc aatacgctgg cggacgaaca tcaggatgtt gccgaacatt
     3541 ttgccgggct gaccgggctg tcgcccgaag agcggtttgc ctaccacatc tggcatcgcg
     3601 gcaaaacggg acaacttgaa atagagggcg cgttggcgca cctgcacggg catattgtcg
     3661 gcaaacatga aatcggcacg cattttgtgt tttacgtcag gctcgacgaa atcaaaaact
     3721 gcgggtgcaa acgccccgcg ctgctgtatt tcagacggca gtttagattt ttagactgat
     3781 attcggacag atatatgaaa gcgatgatac tggcggcagg acgcggcgag cgtatgcgcc
     3841 ctttgaccga taccactccg aagccgctgc tcgatgtggc gggtaagcct ctaatcggtt
     3901 ggcacctatg ccgtctgaag caggcggggt ttaccgaaat cgtcatcaac cacgcttggc
     3961 tgggccggca gatagaagat gctttgggcg acggctcggc ttatggcgtg aacatcgcct
     4021 attcgcccga acccgcaggc ggtttggaaa cggcaggcgg catcgcgcag gcattgccgc
     4081 tgttgggtgg gcagccgttt ttggtggtca acggcgacgt gctgaccgac atcgatttta
     4141 ccgccgcgtt tcaggcggca tcgtccctgc cggaacatat ttccgcccat ctgtggctgg
     4201 tggaaaatcc gccgcacaac cccgacggcg atttttccct gctgcccgac ggcagcgtgc
     4261 ggccggaagt aaatggcggc aacggattga cattcagcgg cgtgggtatt taccgtcctg
     4321 aaatgtttga cggaatcgaa gcgggcagtg tggcgaaact cgcgcccgta ttgcgtggcg
     4381 aaatgcggca aaaccgcgtg agcggtcaga agcatacggg cttgtggctg gatgtcggca
     4441 cggtatgccg tctgaaagag gctcaagccc ttgcaggggc ttggaagtga aataaaacgg
     4501 tggcttgccg agccggctgc cgaacggttc cgaccggatg tccggcatat tttcgtggag
     4561 gcgtgaaaat agggcgttcg gggctttccg tccaacaaat gccatctgaa gcgcggcggt
     4621 ttgccgtcgg cgcggtgtcg gattttattg ggtaactttg ttcagaccgc cgcccgtcag
     4681 attgtcaaaa cccggtttca ggcggtatgg cggattcggt ttaacgtttc aacgccagca
     4741 ccaacacgcc cgccgttacc agtcccaagc ctatccattc ctgcgtgttc gggcgttcgt
     4801 ccaaaaagac caccgccatc agggcgacca ggaccaggct gaatttgtcg acgggcgcga
     4861 cttgcgaggc gttgcccagt tgcagggctt tgaagtaggc gagccaggat gcgccggtcg
     4921 ccaaacccga cagaattaag aatgtccagt tttttcctgt aaatccgttc acgccctgcc
     4981 atttgccggt gtaggtcaaa aacagtacca aagcggcgag gatcaccaag gtgcggataa
     5041 aggtggcgaa atccgaatcg atgccctgca aacccatttt ggcgaaaatg gcggtcagcg
     5101 aggcgaagcc tgccgatgcc aatgcccaaa acagccatgc gttgctgccc atgttttctc
     5161 cttggattgt gaacaatatg aacggtattt ttgttgcagc gtcaaaaatt tcactgcggg
     5221 tttggtgcgg ataacgttat aatatgcctg atattatttt caatccacct gtttgtcgcc
     5281 tgatgctttc agacggtatg tccctcctca tttctaaagg aaaatcatga gcttcaaaac
     5341 cgatgccgaa atcgcccagt cttccaccat gcgcccgatt ggcgaaattg ccgccaagct
     5401 gggcctgaac gccgacaaca ttgagcctta cggtcattac aaagccaaaa tcaatcctgc
     5461 cgaagcgttc aaactgccgc gaaaacaagg caggctgatt ttggttaccg ccattaatcc
     5521 gactcctgcc ggcgaaggta aaaccaccgt aaccatcggt ttggcggacg cattgcgcca
     5581 tatcggcaaa gactctgtga ttgctttgcg cgagccttct ttgggtccgg tgttcggcgt
     5641 gaaaggcggc gcggcaggcg gcggctatgc ccaagttttg ccgatggaag acattaatct
     5701 gcactttacc ggcgactttc acgccatcgg cgcggcgaat aacctgctcg ccgctatgct
     5761 cgacaaccat atctaccaag gcaacgagtt gaacatcgac cccaaacgcg tgctgtggcg
     5821 gcgcgtggtc gatatgaacg accgccagtt gcgcaacatc atcgacggca tgggcaagcc
     5881 tgttgacggc gtgatgcgtc ctgacggttt cgatattacc gttgcttccg aagtgatggc
     5941 ggtattctgt cttgccaaag acatcagcga tttgaaagag cgtttgggca acatccttgt
     6001 cgcctacgcc aaagacggca gccccgttta cgccaaagat ttgaaagcga atggcgcgat
     6061 ggcggcattg cttaaagatg cgattaagcc caacttggtg caaaccatcg aaggcacgcc
     6121 cgccttcgta cacggcggcc cgttcgccaa catcgcccac ggctgcaact ccgtaaccgc
     6181 aacccgtctg gcgaaacacc ttgccgatta cgccgtaacc gaagcaggct tcggcgcgga
     6241 cttgggcgcg gaaaaattct gcgacatcaa atgccgcctt gccggtttga aacctgatgc
     6301 ggctgttgtc gtggcgactg tccgcgcttt gaaatacaac ggcggcgtgg aacgcgccaa
     6361 cctcggcgaa gaaaatttag acgctttgga aaaaggtttg cctaatctct tgaaacacat
     6421 ttccaacctg aaaaacgtat tcggactgcc cgtcgtcgtt gcgctcaacc gcttcgtgtc
     6481 cgactccgat gccgagttgg cgatgattga aaaagcctgt gccgaacacg gcgttgaagt
     6541 ttccctgacc gaagtgtggg gcaaaggcgg cgcgggcggc gcggatttgg cgcgcaaagt
     6601 cgtcaacgcc attgaaagtc aaaccaataa cttcggtttc gcctacgatg tcgagttggg
     6661 catcaaagac aaaatccgtg tgattgccca aaaagtgtac ggcgcggaag atgttgattt
     6721 cagcgcggaa gcgtctgccg aaatcgcctc gctggaaaaa ctgggcttgg acaaaatgcc
     6781 gatttgcatg gcgaaaaccc aatattcttt gagcgacaac gccaaactgt tgggctgccc
     6841 cgaaggcttc cgcatcgccg tgcgcggcat caccgtttcc gccggcgcgg gcttcatcgt
     6901 cgcgctgtgc ggcaatatga tgaagatgcc gggtctgccg aaagtgccgg ccgccgagaa
     6961 aatcgatgtg gacgaacacg gcgtgattca cggcttgttc tgaacggttt ttgaaaccag
     7021 atgccgtctg aagccgtttc agacggcatt ttttcggaac gcgggcggcg gtatgctata
     7081 atccgccgtt aaatttctct attttcagga aaaaacatga gtttgaaatg cggcatcgtc
     7141 ggtttgccca acgtcggcaa atccaccctt tttaacgcgc tgacccaatc gggtatcgaa
     7201 gcggcaaact atcctttctg taccatcgaa cccaacgtcg gcatcgtcga agtccccgat
     7261 ccgcgtatgg ccgaattggc aaaaatcgtc aatccgcaaa aaatgcagcc tgccatcgtc
     7321 gaatttgtcg atattgccgg tttggttgca ggcgcgagca aaggcgaggg cttgggcaac
     7381 cagttccttg ccaacatccg cgaaaccgat gcgattgtga atgtcgtgcg ctgctttgac
     7441 gacgacaaca tcgtccacgt tgcaggccgc gtcgatccga ttgccgacat cgaaaccatc
     7501 ggcacagagt tggcacttgc cgacctggca agtgtggaaa aagccatcgt ccgcgaagaa
     7561 aaacgcgccc gttcaggcga caaagacgcg caaaagctgg tcgatttgtg caaaaaactg
     7621 ctgccgcatc tggacgaagg caaacccgtg cgttccttcg gtttggacgc ggaagaacgc
     7681 gcgatgctca aaccgctgtt cctgctgacc gccaaaccgg cgatgtatgt gggcaacgtc
     7741 gccgaagacg gttttgaaaa caatccgcac ctcgaacgcc tgaaagaatt ggcggcaaaa
     7801 gaaaacgccc ccgtcgtcgc cgtttgcgcc gcaatggaaa gcgaaatcgc cgaattggaa
     7861 gacgacgaaa aagccgagtt cctcgccgaa atgggcttgg aagaaccggg cctgaaccgc
     7921 ctgatccgtg ccggctacga ccttttgggg ctgcaaacct actttaccgc cggcgtgaaa
     7981 gaagtccgcg cttggacgat acacaaaggc gacaccgccc cgcaagccgc cggcgtgatt
     8041 cacaccgact ttgagcgcgg cttcatccgc gcccaagtca tttcttacga cgactttgtc
     8101 tcgctcggcg gcgaagccaa agccaaagaa gccggcaaaa tgcgtgtgga aggcaaagaa
     8161 tacgtcgtgc aggatgggga tgtgatgcac tttttgttta acgtgtaacc caaatgcggc
     8221 aggttttcag acggctttgc cggaaatgcc gtctgaagcc ggttttggtg gttttcggcg
     8281 tttccatacc gccggaatgc agccgtatca aaatatagcg gattaacaaa aatcaggaca
     8341 aggcggggca acgctgtacc ggtttttgtt aatccactat aaaatcccac ccgcatttcc
     8401 gatttgccct cctcgattcc tgcaaaacaa accgcctgcc ccgctgttgc aaaaagccgt
     8461 ccggcattcc gaatatcccg agccccgata caaaatgacc tttcagacgg catttgcgcc
     8521 gccgcgtttc gagtatagtg gattaacttt aaatcaggac aaggcgacga aaccgcagac
     8581 agtacagata gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata
     8641 aaaacattat gagccaagcc ttaccctacc gcccggacat cgacacattg cgcgccgccg
     8701 ccgtcttgtc cgtcatcgtg ttccatatcg aaaaaaattg gctgccgggc gggtttctcg
     8761 gtgtcgatat atttttcgtg atttcaggct ttttgatgac gatgattctt caccg
//
LOCUS       DAWIVB010000071         8780 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWIVB010000071 DAWIVB010000000
VERSION     DAWIVB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.071"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            106..354
                     /locus_tag="WIX60_001157"
     CDS             106..354
                     /locus_tag="WIX60_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ5305430.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            351..737
                     /locus_tag="WIX60_001158"
     CDS             351..737
                     /locus_tag="WIX60_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ5305431.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(917..2272)
                     /gene="yegQ"
                     /locus_tag="WIX60_001159"
     CDS             complement(917..2272)
                     /gene="yegQ"
                     /locus_tag="WIX60_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ5305432.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVSDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            2597..3385
                     /locus_tag="WIX60_001160"
     CDS             2597..3385
                     /locus_tag="WIX60_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ5305433.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAEAGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGWEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            complement(3431..4030)
                     /locus_tag="WIX60_001161"
     CDS             complement(3431..4030)
                     /locus_tag="WIX60_001161"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ5305434.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKLGSRVLLVDDLIATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            4158..4775
                     /gene="gmk"
                     /locus_tag="WIX60_001162"
     CDS             4158..4775
                     /gene="gmk"
                     /locus_tag="WIX60_001162"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ5305435.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTGAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4834..5040
                     /locus_tag="WIX60_001163"
     CDS             4834..5040
                     /locus_tag="WIX60_001163"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ5305436.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            5132..7288
                     /locus_tag="WIX60_001164"
     CDS             5132..7288
                     /locus_tag="WIX60_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ5305437.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(7355..8539)
                     /gene="coaBC"
                     /locus_tag="WIX60_001165"
     CDS             complement(7355..8539)
                     /gene="coaBC"
                     /locus_tag="WIX60_001165"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ5305438.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILKGKKVLITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAENMHRAVHRLIDKQDAFISVAAVSD
                     YRVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMTYAR
                     EKRIKKKLPMIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMRIVERLAVYL
                     SK"
BASE COUNT         2196 a         2029 c         2366 g         2189 t
ORIGIN      
        1 caaggtgtga ataaaacggc ggatataatc aaatatatgt aatgagattc ccaaaatctt
       61 ggtaatgatg tatattagtc aagttagcta atcatgagaa gtgttatgtt tcaagcaaat
      121 attcatcagg caaaaaccaa tttgagtcaa ttgattcaaa gagcagaagc aggggaaatc
      181 gttattattg cgaaggcagg taagccttgc gtccaactaa tcggtattga aaaaccggca
      241 cgaaatgcag gaaggttgaa aaaattcagt catatggaaa atacggatat ttcacgtatt
      301 cttgaggatg acaatgaaac ggcagcttta ttttttgagg agtcggctct gtgagaaaga
      361 ttctgcttga tacccatgcg ttgctgtggt ggttgttgga tgacaagaaa ctggggatat
      421 ctgcacgcaa actgatagaa aatccgagaa atgcgatctt tgtcagtgca gcaagtattt
      481 gggaaatttc catcaagcag aacaaggggt tgttgaaatt accggaagag ttttttgatg
      541 tgcttcaaga agaggatttt gaaatgttgc ctataggtct gtttcatgca aaacaggctg
      601 gaagccttcc ggagatacac aaagaccctt tcgataggat gttgattgca caaacccaag
      661 cggaaggctt tgaactgatg actgtcgatg aatatattcc gcaatatgga attagagttg
      721 tcaacgcatc aagctgacaa atacaaaaac tgtgtacaca agaaagaccg ccgaggcggt
      781 cttttcggtt attgtcttcg cttaaaagcc agacttctgt cgtggactat aggtgtgtcc
      841 cgatctgaat ctgccgcaca ataaggcaag gggattaaaa atgccgtctg aaaaatgttt
      901 cagacggcat aatggcttag gggttcaaca cgcgtgcgat gagggctttt tctttaccct
      961 gcatattggg gattttgacc tgaatgccgt tgcccggggc aacatcgaca ggctggcctt
     1021 tgcgggtcat ttgttccaat ttgatggttt ggttgccgct cgggtggatg atttcgagtg
     1081 aatcgctgac ggcaaagcgg tttttgactt ccactgttgc ccagccgttt tcatcgattt
     1141 cggtaacgtg tccgacgtat tggctttgtt tggcggtgga atggccggtc aggtagtttt
     1201 gataatcctg agtttggtgg cgttcgagga agccgctggt gtagccgcgg ttggcgaggc
     1261 cttcgagttc gctcaacagg ctgtaatcaa acggacggcc tgcgacggca tcatcaatcg
     1321 ctttgcggta ggactgggcg acgcgtgcaa cataatagag cgatttggta cggccttcga
     1381 ctttgaggct gtccacgccg attttggcga gtttttcgac gacttcgata ccgcgaaggt
     1441 ctttggaatt catgatgtag gtgccgtgtt cgtcttccat aatcggcatc atttcgcccg
     1501 ggcggttgga ttcttcaatc aggaaaactt tgtcggcgta gggatggcgt ttttgaccgt
     1561 tgatgccttc aaagttttgg ttggcttctt cttgggcttt ttcaaagttg aaaccttgca
     1621 gaagctgggc atcgcctgca tcgctttccg tggcattgtg aaccttgtaa tcccaacggc
     1681 aggagttggt gcaggtgcct tggttggggt cgcggtggtt gaaatagccc gacaataggc
     1741 aacggcctga ataagcgatg cacaatgcgc cgtggatgaa gacttcgagt tcgatgtcgg
     1801 ggcattcttg gcggatttcg gcgatttctt ccatactcaa ttcgcgcgac agaataatgc
     1861 gttcgacgcc gatgttttgc cagaatttca cgccccaata gttggtggtg ttcgcctgta
     1921 cggacagatg gatcggcatt tccggccatt tttcgcgcac ggtcataatc aaacccggat
     1981 ccgccataat cagcgcgtcg ggtttcatgg caatcagcgg ctccatgtcg gcaacgaagg
     2041 ttttgagttt ggaattgtgc ggcagggtgt tgacggttaa aaagaatttt ttgttgcgct
     2101 cgtgcgcttc tttaatgcct tgttctaaaa catcaagttt ggcaaattcg ttgttgcggg
     2161 cgcgcagtga gtaacgcggg ctgccggcgt aaacggcgtc tgcgccgtag tcgtaggcgg
     2221 cgcgcattct ttccaatccg ccggcgggca ataagagttc gggtgctttc atcgtgtttc
     2281 cttttcggtt gaaaccccgc cctttagggc agtagaatca gactttattt gggaggggcg
     2341 taaccccttc cgaatcaggg caacatatag ggcgacgctt tatgtgtcgt cctgtgtgtt
     2401 gaaacatgat ttgtatgctt tgtaaaaatg tttcagacgg cattgctgca aatgccgtct
     2461 gaaaagggtt tgaggatggg cggattatcc gcctttttgt attttcggtc aattttgacg
     2521 ggaaatttat aaggatttat caagtatttg cctgcttggg atataatgcg gtagtttgaa
     2581 tgcgagagag aatgtgatga atcctaaaat cgtgtttttc gacattgacg atacgctgta
     2641 ccgaaaatat acggatactt tgcgcccttc cgtgaaaacg gcggtggcgg ctttgcgcgg
     2701 caaaggtata ttgacggcgt tggcaacggg gcggtctttg gcgacgattc ccgaaaaggt
     2761 cagggatatg atggcggaag cgggaatgga tgccgtggta acgataaacg gacagtttgc
     2821 gctgctgcac ggaaaaaccg tgcgcgaagt accgatggat gccggtttga tgggcagggt
     2881 ttgcgcgcat ttggatggct tgggcatgga ttatgcgttt gtcggcggag aggggatcgc
     2941 tgtgtccgcg ctgtcggaat gcgtgtgccg cgccttgaag catatcgcca gcgatttttt
     3001 tgccgataag gattattttt caagcaaacc ggtgtatcag atgctggtgt ttgcggagga
     3061 aaacgaaatg ccgctttggt cggatattgt ggaacgggaa ggcttgaaaa cggtgcgctg
     3121 gcacgaggaa gcggtcgatc tgctgcctgc gggtgcgtcg aaaacggacg gcatcagaag
     3181 cgtggttgaa gcattgggat gggaaatggc agacgtgatg gcgttcggcg acggtttgaa
     3241 cgatgtggaa atgctgtcag aagtcgggtt cggcgtggca atgggcaacg gggaacaggc
     3301 ggcgaaagaa gcggcgaaat atgtttgccc cagcgttgat gaagacggcg tgttgagggg
     3361 cttgcaagat ttgggcgtga tttgaacgca tcataacaac cctgccggtt tcagacggca
     3421 gggtcggttt tcagcccttc atacagcctt cgttttgaag cagggtaaat aagggcgcgc
     3481 cgcttgcacg gatattcttg ccgccttgaa ggtcggtaaa ttccaaaatg gcggcggctt
     3541 cgacaatttc tccgccgagt ttgcggatca gttccagtcc ggcaagcatc gtgccgcccg
     3601 tggcaatcaa atcatcgacc agcagcacgc gcgaaccgag tttgacggca tcggtgtgga
     3661 tttccaccgc agcttccccg tattcgagcg cgtagctttg cgatacggtt tcaaaaggca
     3721 gcttgccttt tttgcggatg gggacgaaac cgacgttgag ctggtaggcg agtgccgcgc
     3781 cgataatgaa gccgcgcgcg tccaaaccgg caacgatgtc gattttctga tccatatagc
     3841 ggtaaaccaa taaatcaacc aaaaggcgga agtattccgc gctttgaagg acgggcgtga
     3901 tgtcgtggaa taagatgcct ttttgcggcc agttttcgat tttgcggatt ttgtcggcaa
     3961 gcgcgccgac actcatagct tcgggatgaa ccagcattgc gtgttccaag tttgatgttt
     4021 ttaacgccat attgtagccg tcagcctgtc gcttgccaaa tcaatcaaaa caatagcggc
     4081 aaaatatggg catcggcaaa taggaaactg ggtgtccatt gtgatacaat cggctggaaa
     4141 atatacaggt aaaaaatatg tccgcttatc gaaaaggcaa catctttatc atttcggccg
     4201 cttccggcac gggcaaaacc acgctggtgt cgcggctgtt ggcaaaccat aacggtttgc
     4261 gcgtttccgt gtcgcacacg acgcgcccgc cgcgtgaagg cgaagcaaac ggcgtacatt
     4321 atcactttgt ttccaaagaa gagtttgagt cgcttatcgc gcaggaagct tttttggaat
     4381 acgccgacgt atttggcaac tattacggca caggcgcgga gggtgtgaat gcgttggcgg
     4441 cggcaggcta tgacgtgatt ttggaaatcg acgttcaggg cgcggcgcag gttcgcgacg
     4501 cgctgcccga agccgtcggc atctttatcc tgccgccttc tttcgacgta cttgccgcgc
     4561 gcctcaacgg acgcgggacg gacagtcggg aagttatcca aaggaggctg tcgaaggcaa
     4621 ggcatgaaat cgagcagtcc gtattgtttg actttgtcgt ggtcaatgac gacttggcgc
     4681 gagcggagga ggatttgcgc catattgtga atgcctgccg tctgaaaagg tcgcggcaac
     4741 tggggtttat tgcagatttg ttggaaaatt cctagaaaac ggcgaaaata cccggtttcc
     4801 caatttaaat atttttgaaa gaaagcaaat aatatggcac gtattaccac cgaagactgt
     4861 accggaaaaa tttccaacca ttttgacctg acattggtag cggctcgccg cgcccgccag
     4921 cttgagaacg gcaacacgcc gcttgtggac gatgtccgca ataacaaacc gaccgttacc
     4981 gccttaaggg aaatcgccgc cggacatatc ggtacagaac tgttgacgcg caataaataa
     5041 attctgccgg aaacgcacgc cggaacactt tgccgccgtg cagtccgacg gtttgaaatg
     5101 aaaaacacat acataccgaa agccgtctga aatgcccgcc ccccaacctt ccgcccctta
     5161 cgaccccctg accgccgaag cgcgtgccct gcttttccat accgcctcct acctcaagcc
     5221 cgaggaacag gcggagcttg aaaaagctgt cgcctatgcg tttcgcgccc acgacgggca
     5281 aacccgcaaa agcggggagc cctacatcac gcatccgatt gccgttgcga cgcagctcgc
     5341 cctttggcat atggacatac agggtctttg tgcaggcgtg atgcacgacg tattggaaga
     5401 tacgggcgtg acaaaagggg aaatggcggc ggtgttcggc aatacgattg ccgagatggt
     5461 ggacggtctg tccaagcttg aaaaactcaa atttgaagat catgcggagc atcaggcgga
     5521 gagtttccgc aaactgattt tggcaatgac caaagatgtg cgcgtgattg tcgtcaaact
     5581 tgccgaccgc ctgcacaata tgcggacgct cggttcgatg cgcccggaca aacgccgccg
     5641 gattgcaagg gaaacccttg aaatctatgc acagattgcc aaccgtatag gtttgaataa
     5701 cgcatatcaa gagcttcagg atttatcgtt ccaaaacctg catcccaacc gctacgagac
     5761 tttaaaaaaa gcgatggaca agagccggaa gaaccggcag gacgttgtcg gcaaagtctt
     5821 gcgcgcattc ggccagcggc tggtaggcgc gaatatagag gccaaaatca aaggcaggga
     5881 aaaaaacctg tacggcatcc atcagaaaat gatggcgaaa aagctgcgct ttgccgaggt
     5941 tatggatatt tacggtttcc gcgtcattgt caacagcatt ccagcctgtt atgccgcact
     6001 cggcgcattg cacaccctct atcagcccaa gcccgggcgg ttcaaagact atatcgccat
     6061 tccgaaaagc aacgggtatc aaagtctgca tacgactttg gtcggccctt acggcttgcc
     6121 gattgaagtt cagatacgta ccaaggaaat ggatgctgtt gccgaaggtg gaatcgccgg
     6181 acattggagc tataaatcat attctaagac ggtcgatcag gcggtgcttc acacaaaccg
     6241 gtggctgaaa aatatcttag atttgcaggc aagcagtgcc aatgccattg agtttctcga
     6301 acacgtcaaa gtcgatttgt ttccgaacga aatctacatc cttacgccaa aaggaaaaat
     6361 cctaactttg cccaaagggg caacgcctgt cgattttgct tatgcggtgc ataccgatat
     6421 cgggcacaaa accgttgccg cacgtatcaa caatatcatg atgccgttgc gtacgaagct
     6481 caaaaccggt gattctgttg aaattatcac atccgaacac gccaaaccca atcccgcgtg
     6541 gttgaatttc gccgtgtcag gcagggcgcg cagcgccata cgccaatata ttaaaaacct
     6601 taaccggcac gatgcggtcg ttttgggaga gagcctctta caaaaagccc tgtccagttt
     6661 gctgcccaaa gatgtcctgc tttcagacgg catcaaggaa aaatatcttg ccgatctcaa
     6721 cgacaagcag acatcgtttg aagaagtgct gtacaacgta gggatggggc ataccctgcc
     6781 tgtttatgtc gccatgcaca ttgccgagtt ggcaggggag catttcggca gcgaggtcag
     6841 gctcagttcc attaaagtcg atgggcagga aagcgggcat attcattttg cagagtgctg
     6901 ccaccctgtt cccggcgatt ccatccgttt gctgttggtt aagggaaaag gcatgattat
     6961 ccatagggat acctgcccga cgttgttgaa gtccgatccc gaacagcagc tggatgcaga
     7021 ctgggaaaat atgaacgggc agaactaccg tgtcgggctt caagtccaat cggaagacag
     7081 ccacggcctg ttggcattaa tggcgcaagc gatttccgat tccggtgcag acattgagtc
     7141 ggtcgaaaca ccgtctaaat cccagtcggg aacggaaggt tttgtcgaat tcaaattctt
     7201 attgaaagtc aagaatctga atcaattgaa tcagattatt caaaatctgc atagtattcc
     7261 atatatccgc aaagtcatca gaagttgaag caggtaaaac gggttgcctt ttgattaaca
     7321 ggcaacccgt tttatggttt atccgttcaa ttgcttattt gctcaaatat acggcaagcc
     7381 tttcaacaat ccgcattgcc gcttcatctt tacttgtttc gggaaaagac agttccccgt
     7441 cgtccccgat aatggtaatc cggttggtcg gtttgcccat tgcgattgaa acatcattgg
     7501 caacgatcat cggtagcttt ttcttaatac gtttttcccg cgcatatgtc attacattct
     7561 ccgtttcagc ggcaaaaccg atgcagaacg gcgggttcgg taatgaggca atagaagcca
     7621 aaatatcggg gttctcatcc aattcgatgg ataacggttt ggcattttta tcttttttga
     7681 atttttgagt actcctattc ttaaccctat agtctgagac ggcggcaaca gaaataaaag
     7741 catcttgttt gtcgattaaa cgatgcactg cgcgatgcat attttcggca ctgacggctt
     7801 gaaccgtatc ggatatgccg aaaggcagcg cggtttgaag ctgtccgtga atcaggctga
     7861 cttctgcacc ggcggcacgg cacgcccgcg ccaaagccac gcccattttc ccgctggaga
     7921 tatttgtgat gcctcggaca gggtcaatgg cttcaaatgt cgcacctgcg gtaatcaaga
     7981 cttttttgcc ctttaaaatt ttcggtgtcc ataaatccgg aagcagatcc agcaattcgg
     8041 caggttccgg catccttccc ataccatttt ctccgcaagc ctgttcgccc aagcccggca
     8101 tatagacagt aatgccgtct gaaaccagtt gtgcgatatt ccgttggttg gcagggttga
     8161 gccacatttc cacattcatc gcgggcgcga tggcaagcgg acatttccgt gcggctgcca
     8221 gactggtcag taggttatct gccacgccgt tacagatttt tgccacggta ttcatacttg
     8281 ccggcgcaat cagaaaaaca tccgcattcc gggtcaggtt gatatgttcc ataccgtttg
     8341 aaccgttgcc gccgtgcgtg tcggtcagga caggattgcc gcttaaagcc tgaaaagtca
     8401 gcggagaaac aaattcagtt gccgagcggc tcataaccac cgtaaccgaa tgcccctgtt
     8461 ttttcagcag tcgcaccaac tcgcaagact tatacgccgc aatactgccc gttacaccta
     8521 aaagaatatg tttgcccatt ttgacaattt ctaattacga agaaaaaaca gcagccattc
     8581 tataacaaag tacggataca ttagataccg tttggataca aaatgccgtc tgagtccccg
     8641 agtttcagac ggcatattca caaaggcgca ccagccggag gagggagagg aaaggattgt
     8701 tggaggcggc gcagtattta gcagaaataa aaaaccttat ccgacagcga catgacgaat
     8761 ttccccaaaa aaatcccgct
//
LOCUS       DAWIVB010000072         8769 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWIVB010000072 DAWIVB010000000
VERSION     DAWIVB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.072"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(41..1132)
                     /locus_tag="WIX60_001166"
     CDS             complement(41..1132)
                     /locus_tag="WIX60_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ5305439.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            complement(1267..1815)
                     /locus_tag="WIX60_001167"
     CDS             complement(1267..1815)
                     /locus_tag="WIX60_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ5305440.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            complement(1970..2341)
                     /locus_tag="WIX60_001168"
     CDS             complement(1970..2341)
                     /locus_tag="WIX60_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ5305441.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            complement(2397..2504)
                     /locus_tag="WIX60_001169"
                     /pseudo
     CDS             complement(2397..2504)
                     /locus_tag="WIX60_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2636..4327)
                     /gene="dld"
                     /locus_tag="WIX60_001170"
     CDS             complement(2636..4327)
                     /gene="dld"
                     /locus_tag="WIX60_001170"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ5305442.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGRALAVV
                     RPGTILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKIGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            complement(4494..4590)
                     /gene="ffs"
                     /locus_tag="WIX60_001171"
     ncRNA           complement(4494..4590)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WIX60_001171"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4851..8684
                     /locus_tag="WIX60_001172"
     CDS             4851..8684
                     /locus_tag="WIX60_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ5305443.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKNNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLIEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQIGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKYILGENWLDE
                     FVKKANNGGVEKVLL"
BASE COUNT         1933 a         2388 c         2436 g         2012 t
ORIGIN      
        1 gcgggaatga cgcggtgggg gtttcggttt ttccgcctgt ttattttgcg gcttcgattg
       61 ccgctatttc tttgcgtagg tgtttgatag cgggggttac gatggcaaca aacattgctt
      121 cgcggacgcg cctttgggcg ggactgcgca tcaggtagcc ttttgcgccg gaatgcaggg
      181 cggcggattg ggcggcggca agtgccagta cggcggcggc ttcgcgcagc ttgagggtag
      241 caaggttgtc gggcgtgccg ctccaagcca agccggcgag ccgttcggtt tctgcccacg
      301 cgccgtccag ccttgttttg aggctgtcgt agccgtcgtc gagatagctg ttgacttcgg
      361 cgttgacgat gttggcgagg cggatgatgc cgaggctgcc gtctatcacg cccgcgccga
      421 tgccgatttg caggaggata aagcctgctt tgatgctttg gatgtagtcg gcaaactgtt
      481 cgggcgcggc gatgatgtct tcgtcgggga taaatacgtc tttgaagttc aggctgaagg
      541 tgcgcgtgcc ttcgagggcg caaaattcgg ggcagttttg caggcttacg ccttcccatt
      601 gtccgccggt gatgaacatg acgtagccgc cgccgatttg ggcggtattc gcccaaatgt
      661 ggtcttcgcc gatgttggac acccacggca gcgcgccgtt gactgtgtag ccgccctcca
      721 cgcgctcggc ttggaggttg tgtttttcga tgtcggcaag gtgtttgacg gtattggaca
      781 tgcccgtacc cgccaatact ttgccttgca ggatgtcggc aaggtatttg tctttgacgg
      841 cccggttggg cgtttggtgc agataccacg cgcaagccgc ctgacaccac gcactgaaag
      901 aggttgcgcc gcattctttg ccgatttcgc gcaatacggc gatttgcgtt gccaaaccca
      961 agccgttgcc gccttcggct tctgtaccga ctgcgccgaa tccaccgatt gcgccgagtt
     1021 cgcgcataaa tgcttcgggg tagtatcctt tgcggtcgat gtcgtccact atgggtttga
     1081 gcttggtttt gacgaattcg gcaacgttgg caatcagggt ttgggcgttc atctttgttc
     1141 cttaaggttt gcggggaaat cgggggcgcg cctgatgcgc ggctgcttcc ctgccgctta
     1201 ggcggcttgt ttttgtttga gggggtgttt ttgaaaaacc gcccgatatt cgggcggttt
     1261 gccgtatcag gcgtaagcct gcaattcggg gttgatttcg gtttgtccga ggttgttgac
     1321 gtagttgcac aaagttgcca aggctacgcc catcacgact tcgactgcct gctgctggtt
     1381 gtagcccgca tcaaaaaatg ctttgagttc ctcgtcggat accgcgcctt tttttgccat
     1441 tacggcttgg gtaaaggcgg cgagcgcgcc gagtttagca tcgtcaaatt cgcctgccgc
     1501 caaagcgcgc gcggctttga cggattgttc ggaaaggagt tttttcaggg ttgcgagttt
     1561 ggtgtgccct gccacgcaaa aaccgcattg gttggtacgg gcggcaataa tctggattac
     1621 ttcgacttcg ccggcggtca ggctgttggc ggcgttgagc ttgccgactt cttggtaaaa
     1681 cgccaaggct tcgggggcgt ttgataatac gccgataagg ttggggataa agccgttgtt
     1741 ttgaagtacc gcctcgacgc gcgctttggc ggcttcgggg gcggtttcga gggtgtgtac
     1801 ggttaaacgt gccattttct ttccttgttc gcaaatattg ggtacgggcg catggtatgc
     1861 atttcggaac ggaataggaa agactgattg gttatgtgct gcaaacgaaa ggttataaga
     1921 aatgccgtct gaacattttt cagacggcac gatggaaaag aaaacgcgct tatcggcccc
     1981 cgcgcccgaa atattgcgcc aatgcggctt ggggattggc tgcctcaacc gtttcgggca
     2041 attcggtata gccgcgcgat aaaaggtggc gtgcataaag gctgttgccc tttgcgccga
     2101 accagacgga aaccgccaag cccaatacgt taaacgccga gtctgtggcg ggcgtgtcgc
     2161 cgtaaaccag ttgggcaaac acagccagca cgaatatcgc gcccgtcagc cccagcccca
     2221 aaccccacat tcttttgaaa cacgcccaca gcgtgccgaa caagagcccc ggccatgacc
     2281 agccttgttt gacggcttgg ggcggcaggg cgggatgggt gtagattttg tatggtttca
     2341 tcgtgtttcc ttttcggttg aaaccctgcc ctttgggaag gtaggatcag actttatagt
     2401 ggattaaatt taaaccagta cggcgttacc tcgccttgcc gtactatctg tactgtctgc
     2461 ggcttcgttg ccttgtcctg atttaaattt aatccactat atttgggagg ggcgcgcccg
     2521 tgtgtcggca tacggtttga aagcgattac ccgacgggga acttcaaacc cgacaatgcc
     2581 gtctgaacgg cgttttgcct tcagacggca ttgcctgcct tcaaagcgga cgcgcttatt
     2641 ccgcccagtt tttctttttg ctggttttgc ctacgcccgg gttgaagctg ttggtcgggt
     2701 caagtttgcg gtaaaactgt ttgagcgcgg gtttggcttc atacaaatga ccgacgttgt
     2761 gttcggcggg atattgcgcg ccgcgttggt ctaaaagatg cagcatttcg tgttccaacg
     2821 ccatgcagtc gttgcctttt ttgatgatgt aatcctgatg gaaaacgtgg cacatgaaat
     2881 gtccatagta gagtttatgg atgattttat tgtcgatttc cgacggcagt ttttcaaacc
     2941 agtcgcgatc gtcgcggcgc agggcgatgt cgagcgcgac caagtcttcc acttcgtcat
     3001 catgcacggc gcggtagcgg atggcggcgg aggcgacggc gaaacggtgc agcatcgcgg
     3061 cttgggtttc ttcggcgttg cactcgaaaa acgcgccgct gtggtgtgca aaatattctt
     3121 ttaagaacgc gcgcgcctca tccacgcctt tcccgcctat tttcagaatc aggtggtgtt
     3181 cgtatttgtc gcggtaatcg cgcatggatt tgggcaggtg gtcgggcagg tatttgctga
     3241 cgaactgcat ggccttgtcg gaaaaatgtt tgggcaggaa gctggctttt ttgccgaacc
     3301 tgtccacgcg cgccttcaag tcaaataatt tcggcagttg gtgcgtaccg aattttttga
     3361 tgacgtaaaa cgtgtctttg ccgtacacgt cggcaatgtc gaaagcgtgg cggtggatgt
     3421 attcgccgga aacaggcagg ctttcaaatt cgcccaaggc ggcgcggcgg atgtcggtca
     3481 gctcgttgat gtcgttcgtg ccgatgtaga acacggcggt ttgtttttct tgcgggaagg
     3541 tgtccaaacg gacggcaaaa accatcagtt tgcccgcgca gcccgaggct tcgtaatggc
     3601 gggcggggtc ggcattgaaa cgcgcggcgg tcggttcgtc cacttggcgg acgtgttcgc
     3661 aataggcgtg gtcgtgccct ttgcccgcgt cttgtttgat gtctttgttt tgataatgat
     3721 gaccttgcag gttggtcagg atttcttcgg gcgtgtcgcc caagtctatg cccaagtggt
     3781 tgaccagttc caacctgcct tcttcgttga tttgggcgaa cagtgccatt tcggtgtagg
     3841 cagggccgcg ctgcaccaat gcgccgccgg agttgttgca cacgccgccc aagacggacg
     3901 caccgatgca ggaagagccg ataaccgaat gcggttcgcg ccccaaaggt ttcagcagca
     3961 attcaagctg gttcagggtc gagccgggca ggcagacgac ttgttcgttg ttgttgatgg
     4021 tttggatgat gttcatccgc atggtgttca caatcacgat gtcgcggtcg taatcgttgc
     4081 cgtcgggggt cgagccgccc gtcaaaccgg tattcgccgc ctgcgtaatc acaatcacgt
     4141 ccgcttcgac gcaggcctgc agaattttcc acatttccag aatggttccg gggcgcacca
     4201 ccgccaacgc cctgccctcg ccgaagcggt agccttggcg gtattgttcg gttttcgcgg
     4261 ggtcggtgat gatgtatttt tcgcctacgg tttgggtcag tcttgacagt aattgtgatg
     4321 cgctcatggc agtttcctta aaattgtcgg caggtgcatt gcacattgga attgttttca
     4381 cattgtagtt atacgttatg gcaaagtaaa gaaaatgccg tctgaacggc tttcagacgg
     4441 catcggtgcg atacgggaac gccggaacat cgaagctccg gcgtttcaaa tagggcggcg
     4501 ggccaaaccc ccggcactgg cgcattggag tgggctgctg gcttccgccc ctgacccggt
     4561 gttccgattt gccatgcggg gagacccgcc tcagagaaac ggcattataa cgggttttct
     4621 gaaaaactca accgttttga tacggtcata cgccggaaac accacctaaa atttatattt
     4681 gataatattg tcaacaattt ctcaaagcgt tattttgttt ctataagggt atttcctgtt
     4741 tcggcattga aaagtatcaa aaattgaact acattatcgc cttttcaaac tcgcctgaaa
     4801 ccgacttttc agacggcatt caaataaaaa ctgccaaaca cggacacacc atgaccacga
     4861 ctaccgcccc tcagcgtatt cgggaaatcc cctacaacta tacttcctac accgaccgcg
     4921 aaatcgtcat ccgattattg ggcgacgaag cgtggcaaat cctgcaagac ctgcgcggtc
     4981 aacgcaaaac cgggcgttcg gcgcggatgc tgtttgaagt gttgggtgat atttgggtgg
     5041 tcgtgcgcaa tccgtatctg gtcgatgact tgctggagca cccaaaacgc cgcgccgcgc
     5101 tggtacgtga aatgcgccac cgcttaaatg aaatccgcaa acgccgcgac gataatcggc
     5161 aagtggatgt gttggttgcc gcagcagaaa aagcagtcga gcgttttgat agcagttttg
     5221 atgaaaccag ccaaaaacgg cggcagattt tggagcgttt gagcaaaatc accaagccgc
     5281 acaatattat gttcgacggg ctggcgcggg taacgcacgt taccgatgca accgactggc
     5341 gcgtggagta tccgtttgtc gtcgtcaatc ccgacacgga ggctgaaatc gcgcctttgg
     5401 tgcgcgcctt aatcgagctg gatttggtca ttattccgcg cggcggcggc acgggttata
     5461 ccggcggcgc gattcctttg gacgcaaaca gcgcagtcat caataccgaa aaactcgaca
     5521 agcatcgtgg tgttgaatac gttgagctgg caggcttgga cggcaagcat ccgattatcc
     5581 ggtgcggcgc gggcgtggtt acgcggcggg tggaagaaac cgcgcatcag gcaggtttgg
     5641 tgttcgccgt cgatccgact tctgccgacg cgtcatgcgt gggcggtaat gtggcgatga
     5701 acgcgggcgg caaaaaagcc gtgctgtggg ggacggcgtt ggacaacctc gcctactgga
     5761 acatggttaa ccctcaaggc gaatggctgc gtatcgagcg cgtgcgccac aatttcggca
     5821 aaatccacga cgaagaaacc gccgtgttcg acgttcacac gctggattca gacggcatca
     5881 atatcgttaa aaccgaacgc ttggaaatcc ccggccacaa attccgcaaa gtcggtttgg
     5941 gcaaagacgt taccgacaaa ttcttgagcg gcctgcccgg cgtgcaaaaa gaaggtacag
     6001 acggcatcat caccagcgtt gccttcgtgt tgcataaaat gccgaaatac acgcgcaccg
     6061 tgtgtatgga gtttttcggt acggtcgcca ccgccacgcc atctattgtc gaaatccgcg
     6121 actttttgct tgcccatgaa agcgtgcggc tggcgggttt ggaacatttg gactggcgtt
     6181 atgtccgcgc cgtcggctac gccaccaaag cggcgggcaa gggacgaccg aaaatggttt
     6241 tgctggcaga cgtggtttca gacgacgaag ccgccgtaga ggcagccgcc gaacacatct
     6301 gtgaactcgc acgcgcccgc gacggcgaag gctttatcgc cgtatcgccc gaagcccgca
     6361 aaaccttctg gctcgaccgc agccgcaccg ccgccatcgc caaacatacc aacgccttta
     6421 aaatcaacga agacgtggtc atcccgctcg aaaggctcgg cgagtattcg gacggcatcg
     6481 aacgcatcaa cattgagctt tccatccaaa acaagctcaa actctgtgcc gccttggagc
     6541 aatatctttc gggcaaactc cccatcgaca aaatgggcac tgacctgccg accgccgaac
     6601 tgttgggcga acgcggcaaa cacgccctgg cccacgtttc cgccgtcaaa acgcgttggg
     6661 aatggctgct cgccaatctt gacacgccgc ttgccgacta caaagcccgc tacggcgcag
     6721 ccgtccacgc cgcacccgaa gccaaaaaca atgaaagctg ctttattgcc ttccgcgatt
     6781 tccgcctgcg cgtgtctgtc aaagcggacg taatgaaacc gctttctgaa atcttcagcg
     6841 gcaaaaccga caccaaaatt atccaaggct tgggaaaaat ccacgcaaaa accgtacgca
     6901 gccgcgtctt tgtcgccctg catatgcacg ccggcgacgg taacgttcac accaatattc
     6961 cggttaactc agacgatgcc gaaatgcttc agacggcata ccgctcagtc gaacgcatta
     7021 tgaaaatcgc ccgttcgctt aacggcgtga tttccggcga acacggcatc ggcattacca
     7081 agctcgaatt tttaagcgac gaagaaatgc agccgttttg ggactacaaa aaccaagtcg
     7141 atccgaaaca caccttcaac cgtcacaaac tgatgaaagg ctcggactta cgcaacgcct
     7201 acacgccgtc cttcgagctg ttgggcgcgg aatcgctgat tatggaaaaa tcaaacctcg
     7261 gcacgattgc cgattccgtc aaagactgcc tgcgctgcgg caaatgcaaa cccgtctgct
     7321 ctactcacgt tccgcgtgcc aacctgctgt acagcccccg caacaaaatc ctcggcgtgg
     7381 gcttattgat cgaagccttc ttatacgaag aacaaacccg ccgcggcgtt tccatcaaac
     7441 actttgaaga actcatggac atcggcgacc actgcaccgt gtgccaccgc tgcgtcaaac
     7501 cctgccccgt caacatcgac ttcggcgacg ttaccgtagc cgtccgcaac tatcttgccg
     7561 attccggcca caaacgattt gcgcctgccg cagctatggg tatggcgttt ttgaacgcca
     7621 ccggcccgaa aaccatcaaa gcccttcgcg ccgccatgat acagatcggc ttcccagcgc
     7681 agaatttcgc ctacaaaatc ggcaaacttc ttccaatcgg cacgaaaaag caaaaagccg
     7741 aacccaaggc aaccgtcggc aaagccccga ttaaagaaca ggttatccat ttcatcaacc
     7801 gcccactgcc caaaaacgta cccgccaaaa caccgcgctc cttattgggc atcgaagacg
     7861 gcaaaagcat ccccatcatc cgcaaccccg ccgcgcccga agatgccgaa gccgtgttct
     7921 acttcccggg ttgcggctct gagcgtctgt tcagccaaat cggacttgcc gttcaagcca
     7981 tgctctggca cgtcggcgta caaaccgtcc tgccgcccgg ctatatgtgt tgcggctatc
     8041 cgcaagacgc aggcggcaat aaggcaaaag ccgaagaaat gagcaccaac aaccgcgtgg
     8101 ctttccaccg tatggcgaac accctcaact acctcgacat caaaaccgtc gtcgtcagtt
     8161 gcggcacttg ttacgaccag ctcgaaaaat accgctttga agaaatcttc cccggctgcc
     8221 gaatcatcga catccacgaa tacctgctcg aaaaaggcgt gaaactcgac ggcgtaaaag
     8281 gtcagcaata cctctaccac gacccctgcc atacccccat caaaaccatg aacgccaccc
     8341 aaatggccag cagcctgatg gggcagaaag tcgttttaag cgaccgctgc tgcggcgaat
     8401 ccggtatgtt tgccgtcaaa cggccagaca tcgccactca ggtcaagttc cgcaaacaag
     8461 aggaaatcga gaaaaacctc aaagagctgc cgcagggcga acccgtcaaa atgctgacct
     8521 cctgccccgc ctgcctgcaa ggcttgagcc gctacgccga cgacaacaat atgcctgccg
     8581 actacatcgt catcgaaatg gcgaaataca tcctcggcga aaactggctg gatgagtttg
     8641 taaaaaaagc caacaacggc ggtgtagaga aagtgttgct gtaacaacgg acacggaaat
     8701 gccgtctgaa cgccgaaagc cttcagacgg cattgtttga accaaatata gtggattaac
     8761 aaaaatcag
//
LOCUS       DAWIVB010000073         8762 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWIVB010000073 DAWIVB010000000
VERSION     DAWIVB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.073"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="WIX60_001173"
     CDS             complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="WIX60_001173"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ5305444.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAKADEGEYFGALFQYVGKDG
                     DVQDLQSVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGIKVVHEVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSAYEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQYRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSTSAGQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKALGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3125..3484)
                     /locus_tag="WIX60_001174"
     CDS             complement(3125..3484)
                     /locus_tag="WIX60_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305445.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3432..3623
                     /locus_tag="WIX60_001175"
                     /pseudo
     CDS             3432..3623
                     /locus_tag="WIX60_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
     gene            complement(3759..4400)
                     /locus_tag="WIX60_001176"
     CDS             complement(3759..4400)
                     /locus_tag="WIX60_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ5305446.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4390..4941)
                     /locus_tag="WIX60_001177"
     CDS             complement(4390..4941)
                     /locus_tag="WIX60_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ5305447.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4945..5463)
                     /locus_tag="WIX60_001178"
     CDS             complement(4945..5463)
                     /locus_tag="WIX60_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ5305448.1"
                     /translation="MRLFPIAAALSLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5463..7124)
                     /gene="pqiB"
                     /locus_tag="WIX60_001179"
     CDS             complement(5463..7124)
                     /gene="pqiB"
                     /locus_tag="WIX60_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ5305449.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7117..8490)
                     /locus_tag="WIX60_001180"
     CDS             complement(7117..8490)
                     /locus_tag="WIX60_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ5305450.1"
                     /translation="MHPDWVYRLFSFIMPPLIFATFPMKPFAENIPHSLRGNCCDEAL
                     PPHTVDCPECGCRADVPRLDSGEAAFCPRCGHKLFRVGRHPFSAPPAYAAASLILMAF
                     AYGMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVY
                     AALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMF
                     ALSVMLIRTSVSVPQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVC
                     GAELYRRRPKSLSISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDE
                     GDRLIAAVIFSASILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMI
                     DIFVIIILMCSFHTYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAF
                     NETEKHD"
BASE COUNT         1964 a         2237 c         2466 g         2095 t
ORIGIN      
        1 ttgtttgtcc gtgtggaaat gacatcaaag ggtgtgaata ggcttttcat ctttgaaatc
       61 tgtctgcccg atcggatgaa taaaaagtag gatgataaaa aagctgatgt atggatactg
      121 gtttaaccca tttgttttaa agttatagtc gagatacgtg tatcggtgca accagtctgc
      181 cgtcgacgaa aatgccgtct gaaagtgttt cagacggcat ttgtcttcgg ggtttcaacg
      241 gtcagtcttc ataattttcc atcggtgggc agctgcacac gagattgcgg tcgccgtaca
      301 cgtcgtccac acggttgacg ctcggccaga atttgtgttc gcggacgaac ggcaatggga
      361 agacggcttc ttcgcgggaa tacggatgcg cccagttgcc ggttatatct gcggcggtgt
      421 gcggcgcgtt gaccagtggg ttgtcgtctt tcggccattc gccgcgcccg actttcagca
      481 cttcctgttt gatttgtttc agggcggcga tgaagcggtc gagttcggct ttgctttcgc
      541 tctcggtcgg ctcgatcatc agcgtaccgg caacggggaa ggaaaccgtc ggggcgtgga
      601 agccgtagtc catcaggcgt ttggcgatgt cggtttcggt aatgccgctt tcggctttga
      661 gcggacgcaa gtcgacgata cattcgtgcg cgacgcggcc gtttttgccg gtgtagagaa
      721 tcggataatc ctcgcccaag gctttggcga cgtagttggc gttgagcaat gcccagcgcg
      781 ttgcctgttc catgccttgt ttgcccatca tggtcaggta catccaagta atcggcagga
      841 tggatgcaga accatatgct gcggcagcca cggcggtttg cccggcactt gtgctgtgcg
      901 tgtcggtcaa agcgtgtccc ggtgcaaacg gggcaaggtg ggctttcaag ccaatcggac
      961 ccatgcccgg gccgccgccg ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca
     1021 acacgtccgc gccgacttcg gcaggctgca tgatgccgat ttgagcgttg aggttggcgc
     1081 cgtccatata aacctgtccg ccgttttcat ggatgatgcg gcagatgtcg cggatgcctt
     1141 cttcatacac gccgtgggtg gacggatagg taatcatgat ggcggacaaa gcgtcgcggt
     1201 attgctcggc tttggctttc aaatcgtcga tgttgacgtt gccgtgttcg tcggtatcga
     1261 cgacgacgac tttcaagccc agcatggccg cagtcgcagg gttggtaccg tgggcggatt
     1321 tgggaatcag gcagatgttg cggtgcgctt cgccttgggc ttcctgatag cggcggatgg
     1381 caagcatacc gctgtattcg ccctgcgcgc cggagttggg ctggaaggaa attgcatcaa
     1441 aaccggtgat ggatttcagg ctgttttcca tatcggcgag caattcgcgg tagccggcgg
     1501 tttgcgcttc aggagcgtaa ggatggatgt cggagaactc cgcccaagta atcggcaaca
     1561 tttccgcagt cgcgttgagt ttcatggtgc agctgcccaa tgaaatcatg ctgcggttca
     1621 tcgccaaatc gcggtcttcg agttttttca gatagcgcaa catttcgtgt tcggtgtggt
     1681 aacggttgaa cacaggatgt tgcagaatgt catcttgacg cagcaattcg gcgttcagac
     1741 ggcctttgac atcatcggca aatgcgaacg catccttgcc ggtaaacgcg cggtacaaat
     1801 caaccaaatc ttcgtatgcc gatgtttcat ggaatgcagc cgcaacttga gtatcgttga
     1861 cgcggcgcag gttgtaaccc gactctaaag cagcggcaaa cacttggtct gctttctctt
     1921 tgttgccgaa atcgacggta acggtatcga agaagacttc gtgaaccact ttgatgccgt
     1981 ctgaaaccag tgcatcggca aaggcagaag ccagcgcgtg aatgcggttg gcgatgcgtt
     2041 tcacgccttc agggccgtgg taaacggcat acataccggc caaattcgcc agcaatgcct
     2101 gcgcggtaca aatattggac gtggcttttt cgcggcggat gtgttgctcg cgggtggaca
     2161 acgccatacg caaggcaggt ttgcccgatg cgtctttgga tacaccgatg atacggcccg
     2221 gggcggaacg tttgaactcg tctttaaacg cgaaataagc agcatgcgga ccgccgaagc
     2281 ccatcggcac gccgaagcgt tgcgtgttgc ccaaagcaat atctgcaccc aattcagccg
     2341 gagacttcag caaaaccaag ctcatgatgt cggcggaaac ggcgacaatc gtgcctttgg
     2401 ctttcagacg gcctataacg ctttgcaagt cttgcacgtc gccgtctttg ccgacgtatt
     2461 ggaacagcgc gccgaagtat tcgccttcat ccgctttggc aaaatcgccg accaccaatt
     2521 caaagccgaa atacttggcg cgggttttca tcacgtccaa agtttgcgga tacacgcgct
     2581 cgtccacgaa gaaacgctcg gattttacct tgcccacgcg gtgcgccatc gccatcgctt
     2641 cggcggcggc agttgcctcg tccagtaaag acgcgcccgc cacagggaaa ccggtcaaat
     2701 cgatgcacac ttgttggaag ttcaacaatg cttccaaacg accctgcgcg atttccgcct
     2761 gatacggcgt gtaggcggta taccagccag gattttccaa tacgttgcgc aaaatcacat
     2821 tcggcacgcg ggtcgggtaa tagcctaaac cgatatagga cttgttgatc acgtttttcg
     2881 acgcaatgcc tttcaatttc gccaatgcgt ccgcctcggt caaggcctcg ggcaaatcga
     2941 gttcagacgg catacggatg ctttgcggca cggtgttgcc gacaaaatcg tccatacttt
     3001 tctcgccgac agcggcaagc aacgccgctt cgtcgccgaa actcaaatgc cgcgcggcaa
     3061 attcgttggg gttgaacagt tcggacagtt tcatttctac tccttcgggt ttgtagggct
     3121 gccatcaggc agaattttta gacatcggat gattcgggca agggtagcac aaaagacggg
     3181 ataatggcgg acaatgcgcc agaatgcagc aggtcaggac aagcagcgcc acaagcggca
     3241 gcgataaggg aaagccgccg cccaaaacca tgaaacctgc aggcaccagc caagccaatg
     3301 gatacgccgg aagcggaaaa acaatattga gcagaaccaa aatcacgaca aacagcggca
     3361 gacccgccat caggttggca cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt
     3421 cgcaatattc catgatttca gcctgtccgg gctttatcca cagcaggctc ggacggtcgg
     3481 tcatgccgtc tgaaccggca ggccgtcttt ttcaaaaaac gccataccgc tgcaacttca
     3541 tgcggctgta tggcgtaggc tttgcacttc agacggcata tgcgtcccga agcagtttgg
     3601 tgttccagtc ggttcatgat tatagtggat taaatttaaa ccagtacggc gttgcctcgc
     3661 cttgccgtac tatttgtact gtttgcggct tcgtcgcctt gtcctgattt ttgttaatcc
     3721 actatatata aacaacttgg gtgcggataa acctttgttt atctaaaccc ctgttttctc
     3781 aaaaattgat tcaaatcttc ggcaaatttc cgatagcctt tgccgttggc gtggatttgg
     3841 tcggatttca gattattatc gcccaaaatt tccgcccacg cgccgccgaa cagcggaatg
     3901 ccgtattcct cggacaaatc ctcatacagc ggatgatcgc tcaaatgccc gaacaacgca
     3961 cccagtgtga tgtgcggcac gccgacgagg acggcgggga tgttttcctt ctgcacggtt
     4021 tcgatgattt tcgcgatatt ggcgcgggtc tgctccttgg gaactttgcg cagaaagtcg
     4081 ttgccgccta tgccgacaat cacaagcttg ggtttgcgtg ccaacagcgc gggcaggcgc
     4141 gacagggctt gggcagatgt atcgcccgat acgccgccgt tgacaatatt ccaacccgtc
     4201 agtttttgca gttgcgcggg gtaggattcg ccagggtttg cgccgtagcc gaaggtaagc
     4261 gaatcgccca aggcaagtac ggtgcttcct tcgggaattt tggcgtgggt tcgggcggat
     4321 tttctgccgc aggcggtaag cagcaacgcg cctgcgccga ggaggaaggt tcttctgttc
     4381 attggttttt cagatggcat cgagccattt cgggtggtat ttcagggttt cacggtaaac
     4441 cggacagctt tcggcgtgcg cgcctttgag gtagccggta ctcatcagaa attcgccgac
     4501 gatttcgccg ccgacgaatt tgaaatgttt tttaaagagt ttaacccatt cgtctttgct
     4561 tcgcggatgg tgcgtgtcga gccagttctt gaacgagccg tattcttgtt gcaacgcttg
     4621 gatttgccgt gcattgaaaa tggcggcatc gattttcagg cggttgcgga caatgcccgc
     4681 gtcggcaagc aggcgttcgc ggtcggtgtc gtcgaaggcg gcaaccgtat cgatgtcgaa
     4741 accttcaaat gccgtctgaa acgcctgccg cttcttcagc atcagcgtcc agtttaatcc
     4801 tgcctgattg atttccaaca ccagccgctc aaacaattca ttgtcgtcct caatcggaaa
     4861 accgtattgc gtgtcgtggt aatgtttgtt cgggttatcg gtgttttcgg gaagtgaggc
     4921 ggcaaattcg caatagttca cggtttactc gaccatctgt tgcgccgcct gtttcagtcc
     4981 ctgttcgagt gcggcggtca tcgcggcgta gccgtcaccc tgctgttcgg tttcgatatg
     5041 gaagggtctg ttcgtaccgt cgggtaggac ggcgtagccg ctgatgaggg ttttgcccgt
     5101 gtagctgcct tggaatgcgt cgatatagac cgtccatttt tcggtactgc cgctgcgtga
     5161 ggcaggaaca aagatgcgtg tgctgtccaa acggttgaat gcattgctca acgccgcttc
     5221 gagcatatcg tccaaggtgt ctgcccagac gtggttttgt gcggtgttga ggcggtaggg
     5281 gtcggtttga tagaccagtc cgccgcgttt gagcggttcg gcaagacgga cttcgacggc
     5341 agtttcgccg ccttgcgttg caggacggat gtagcggctg tcgggcaaca cgaaatattg
     5401 tgtgctttgc acagtaccgc aggcggcaag cgacagggcg gcggcaatcg ggaaaaggcg
     5461 cattatcggc ttcctttcgg gatagggtct ttgctgctgc tgttgaaaat cagcgcgttg
     5521 ggtttttctt tcaaagtatt aatcacgggt tgaacgtctt ttaaagtttt gtccaaactt
     5581 tgcagcgtat tttgtacgtc gccgtagata ggcgattgcg gcgatacgcc ttgcagggtt
     5641 gtgcgcaact ctttcagggt ttggttcagt tcgttcggaa tgttttgtgt ctgcggtttg
     5701 ccgaccagtt tgtcgatgga gcttagggcg gcattggcag atttgagtgt ggatttgagc
     5761 tcggcaagcg aaccgttcaa ttcggcaacc gtcttatcta aaggcagttt gtcgaacttg
     5821 tccagcaaat ccgccaattt gacctgcaaa tcgtccaaac cgccgccctg ggtcgcgata
     5881 acggtatcgc ctgcataaac ggtatgcggt cgcagcttag gtgatgcgga aggctgatcg
     5941 ttcaactcaa tcattttgct tccggtcagc aggttgttgc tggagatggt ggcggtcagg
     6001 cctttgttta aggccgtctg aaattgttgt ttccaatgtt ctttgctttg ttcgtcggca
     6061 ttgatttcca aacgggaagg ttcaatgcgg atgcgtacgg gtatccagcc gttttcaaac
     6121 aggtgcaggc tgtcgttgcg gtcgaaataa ggaacgtcgg aaaccacgcc gacattcagc
     6181 cctttgtact cgacgggcga accgacggtc aggccgcgca cggattgttt gaaaaacgcg
     6241 gtgtagtaca gcgagcggtc gtcaggcagg ttggcgactt cgctgcggct gtcgtaaagc
     6301 gtgaagctgt cttcgctttt gacgttttta ctgtttttgg ttttcggcga atcaaatgaa
     6361 atcgcgcccg acagcagggc aggcagaggg gcggaattga gtttgatgcc gctgcctgtg
     6421 gtttcgatat tgatgccgct ttccagccaa aaacggctgg cggaatgaat cagtttgtcg
     6481 ttggggcttt ggatgaagat ggtgtaatgc acgctttggt cggacgggtc gaaatgcgcg
     6541 ctttcgactt gcccgaccat aaaattttca tacaaaacag ggctgttgac gttgaggatg
     6601 cggtcgtttt taccaatcaa attcaagcgc agcccgcttt gcccgatggc ggtaacgggc
     6661 ggaatgtcct gcacttggaa cacgtctttt gcctcgtcgc ttttgccggg tgtaaaggcg
     6721 atgtacgaac ccgaaagcag cgtacccaaa ccggttacgc cgctttggtc gatacgcggc
     6781 ttgaccaccc aaaactgggt atcgctgcgg atgaggccgg atacgtccgc attgagttgg
     6841 gcggttactt ccacgccttt ttggtcgtcg cgcagtttga ttcgggtaac gcgtccgaca
     6901 tcgatgctca atactttgat gaccgtattg ttgacctcaa tgccttccgc gctgtccatc
     6961 aagagcgtaa ccacaggccc cctgttgcgg atttccttaa cccaaagcca gccgccggca
     7021 atcagcgcga tcagcggaac cagccagacg gcagagagga aggtgttgtt tttgcggacg
     7081 cgtgcttggg cgtgtccgtt tggaggaggg ctgttgtcag tcatgttttt ccgtttcatt
     7141 gaaagcaatg ccgtctgaag cgcgtttgtc ccaaagcagg cgcgggtcga aataataggc
     7201 ggacagcatc gtcagaatca cgaccaggca gaaatagact gccgcactgc ccggaatgac
     7261 gcgcgcggca taagtgtgga acgaacacat caaaataata atcacaaaaa tatcaatcat
     7321 cgaccagcgg ccgaccgctt cggtgatgcg gtagaggtgc gacaatttct ttgcacccgt
     7381 tggcaaagcg aagcgggcgg acgcaatcaa aaccgacatt gccgcaatct tcagtaccgg
     7441 caccaaaata ctcgcgctga aaataaccgc cgcaatcagc ctgtcgccct cgtcccacat
     7501 ataagcgatg ccgttaagga tggtattgac ctccgtggcg gcaggattgg acgaaatcat
     7561 aatcggcagg atattggcag ggaaatacaa aataaccgcc gccgtcagaa acgccgacga
     7621 aatactcaga cttttcggcc gtcggcggta cagttccgca ccgcacacgc cgcaggggga
     7681 ttcggcactg tcgcggaaat acaggcagcg gctgcaacag gttttacctt ccgatgccgt
     7741 ctgaaccgca ttatcccccg tcagccgccc gatttgaaaa tacacccaat gctggggaac
     7801 cgataccgaa gtccgaatca gcataactga cagcgcgaac atcagataaa acgccggccc
     7861 gaagcgaacc tctgccacag acgagagctt gatatacgcc accaaagtgg aaacaaaaaa
     7921 cacatccacc atcatcgcct gtctcaagcg caccatcaca cgcgttgcca aacgcagcgc
     7981 aggatacgcc tgtttccgta tcagcgcggc atagacatac aggcacagca gcagaaacag
     8041 aaccggcgcg ccgaaagtca gcacaaacat cacttcggcc aaaaaaccat aatcctgaaa
     8101 caccatcagg cgcatcatct cgggcagcga aaggacggat gccgcacccg gtatcccgac
     8161 ctcgatatac gtcataccgt aagcaaacgc cattaaaatc agcgaagccg ccgcataggc
     8221 gggcggggcg gaaaaaggat gcctgcccac cctgaagagt ttgtgtccgc aacggggaca
     8281 gaacgccgct tctccactgt ccaaccgggg tacatccgcg cggcagccgc attccggaca
     8341 atctaccgta tgcggcggca gggcttcgtc gcagcagttg ccgcgaaggc tgtgggggat
     8401 attttcggca aacggcttca tcgggaaagt ggcaaatata agtggcggca ttataaagga
     8461 aaaaaggcga tacacccaat caggatgcaa aaccggcagg ttcggcagta tgaacaaacc
     8521 cttggtgtcc ggcaaaaata ttttttgagc ggttgtccgc cgactgccga acccatttga
     8581 taaaatcccg attctgtaaa gaatagtaaa atacctgctt aaaaacatca tgcaaatgat
     8641 attttttcca aaaatatgtg ataataaatt tcgtttatga ttctttatta tttttatagt
     8701 ggattaacaa aaatcaggac aaggcggcga agccgcagac agtacaaata gtacggaacc
     8761 ga
//
LOCUS       DAWIVB010000074         8746 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWIVB010000074 DAWIVB010000000
VERSION     DAWIVB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.074"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..164)
                     /locus_tag="WIX60_001181"
     CDS             complement(<1..164)
                     /locus_tag="WIX60_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEZ5305451.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIE"
     gene            403..1302
                     /gene="crgA"
                     /locus_tag="WIX60_001182"
     CDS             403..1302
                     /gene="crgA"
                     /locus_tag="WIX60_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ5305452.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLSVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIVCLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1527..4742)
                     /gene="carB"
                     /locus_tag="WIX60_001183"
     CDS             complement(1527..4742)
                     /gene="carB"
                     /locus_tag="WIX60_001183"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ5305453.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQVVHSAEELQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4771..5412)
                     /locus_tag="WIX60_001184"
     CDS             complement(4771..5412)
                     /locus_tag="WIX60_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305454.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            complement(6094..6483)
                     /locus_tag="WIX60_001185"
     CDS             complement(6094..6483)
                     /locus_tag="WIX60_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ5305455.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQYAQ"
     gene            complement(7056..7181)
                     /locus_tag="WIX60_001186"
                     /pseudo
     CDS             complement(7056..7181)
                     /locus_tag="WIX60_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7227..8360)
                     /gene="carA"
                     /locus_tag="WIX60_001187"
     CDS             complement(7227..8360)
                     /gene="carA"
                     /locus_tag="WIX60_001187"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ5305456.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYASGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIKAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         1948 a         2237 c         2309 g         2252 t
ORIGIN      
        1 ttttcgattt ctgcctcaac atcatagccg gcatcaatca cggtttcttg aacattgtgt
       61 ccgagcgcgg tcaaaacttc tttcgctttt ttgtgaagcg tacggtttaa cccgccgtga
      121 gaatgtccga acgccttgcc gccgtctaat aataaaatat tcatttgtta cctcgtttgt
      181 gaattgatga atgtattgtg ccgttttact ttataattta aaagtgcaaa aataagaaaa
      241 cacttttgcg tcaaatgaaa taatcagatg ttccaacaca cgggatggca cataaagcac
      301 cgccctgtgt gccgtcctga ttcggaaggg gttacacccc tcccaaataa agtctgatcc
      361 tgccgcccta aagggcgggg ttccaaccga aaaggaaaca cgatgaaaac caattcagaa
      421 gaactgaccg tatttgttca agtggtggaa agcggcagct tcagccgtgc ggcggagcag
      481 ttggcgatgg caaattctgc cgtaagccgc atcgtcaaac ggctggagga aaagttgggt
      541 gtgaacctgc tcaaccgcac cacgcggcaa ctcagtctga cggaagaagg cgcgcaatat
      601 ttccgccgcg cgcagagaat cctgcaagaa atggcagcgg cggaaaccga aatgctggca
      661 gtgcacgaaa taccgcaagg cgtgttgagc gtggattccg cgatgccgat ggtgctgcat
      721 ctgctggcgc cgctggcagc aaaattcaac gaacgctatc cgcatatccg actttcgctc
      781 gtttcttccg aaggctatat caatctgatt gaacgcaaag tcgatattgc cttacgggcc
      841 ggagaattgg acgattccgg gctgcgtgca cgccatctgt ttgacagccg cttccgcgta
      901 atcgccagtc ctgaatacct ggcaaaacac ggcacgccgc aatctacaga agagcttgcc
      961 ggccaccaat gtttaggctt caccgaaccc ggttctctaa atacatgggc ggttttagat
     1021 gcgcagggaa atccctataa gatttcaccg cactttaccg ccagcagcgg tgaaatctta
     1081 cgctcgttgt gcctttcagg ttgcggtatt gtttgcttat cagatttttt ggttgacaac
     1141 gacatcgctg aaggaaagtt aattcccctg ctcgccgaac aaacctccga taaaacacac
     1201 ccctttaatg ctgtttatta cagcgataaa gccgtcaatc tccgcttacg cgtatttttg
     1261 gattttttag tggaggaact gggaaacaat ctctgtggat aattctataa aaacctgtca
     1321 taaaactgtt tttactcact taataacttt gagaaaaaat aaataagata atgtatagtc
     1381 ggcggcaatg gcaatctata tggggagcgt gatagggctg ttccttatgt acggcttgtg
     1441 tgtggcaaca tcggctttcc aaatgacaaa atgccgtctg aaaccgcaaa acggcttcag
     1501 acggcatttt caacctgatt caggcatcag ttgcggtttt tcaaacgccc gtgcagttct
     1561 tgaacgctgt acacgcccag atggtctcgg cttttcgcgc cttcgctcat cgcttcgccg
     1621 ccggcggtgg tggtgtattg cggcacacgt tgctgcaatg cgctttggcg gatgatgtgg
     1681 ctgtcggaca cggattgcgg atcgctggaa acggtgttca cgaccagtgc gatttcgccg
     1741 tttttcagcg cgtcgccgat gtgcgggcgg ccttcgggta ctttgttgat ggtctgcaca
     1801 atcagcccgt gttcggtcag gtattgcgcc gtgccgcgcg tggcgcagat gccgtagcct
     1861 aaaacttgga agtttttagc ggttttaatg acgcgttctt tgtcttcttc gcgcacggag
     1921 aggaagattt tgccggtcgg gttgaggcgt tcgcccgcgc cgagttgggc tttgtagtag
     1981 gcttcgccaa agcttgcgcc cacgcccatg acttcgccgg tggagcgcat ttccggtccc
     2041 aaaatcgtat ccacgcccgg gaatttgatg aatgggaaca cggcttcttt aacggcatag
     2101 aaatcgggga caacttcttt ttccacgcct tgttctttca gggaaatgcc tgccatgcag
     2161 cgcgcgccga ctttggcgag cggcacgccg gtggctttgg agacgaaggg cacggtgcgg
     2221 ctggcgcgcg ggttcacttc caatacgaac actacgccgt cttgtacggc aaactgcacg
     2281 ttcatcagtc cgaccacgcc cagcgcgtac gccatcgctt tggtttggcg gcggatttcg
     2341 tcttggattt cttcgcttaa ggagtagggc ggcagcgagc agccggagtc gccggagtgg
     2401 atgcccgcct gttcgacgtg ctgcatgatg ccgccgataa ccacgtcttt gccgtctgaa
     2461 acgcagtcca catccacttc aatcgcgttg ttcaggaaga agtcgagcaa cacggggctg
     2521 tcttcggaaa cctgcacggc ttcgcgcatg tatttttgca gctcttcggc ggaatggacg
     2581 acctgcatgg cgcggccgcc gaggacgtaa gacgggcgca cgaccagcgg atagccgatt
     2641 tcttcggctt tgacgagcgc ttcttcttcg ttgtgggcga tgcggttggg cggttggcgc
     2701 aggcctaagt cgttcaacac tttttggaag cgttcgcggt cttcggcggc gtcgatgctg
     2761 tcggcggacg tgccgatgat gttcacgccg ttttcaacca gcgcgttggc gagtttgagc
     2821 ggggtttggc cgccgtaatg cacaatcacg ccccacgggt tttcggtgcg gacgatttcc
     2881 aacacgtctt ccagcgtcag cggctcgaaa tacaggcggt cgctggtgtc gaagtcggtg
     2941 gacacagttt cggggttgca gttgaccatg atggtttcaa agcccgattc gcgcagggcg
     3001 agcgcggcgt gaacgcagca gtagtcaaac tcgatgccct gaccgatgcg gttcgggccg
     3061 ccaccgagaa tcatcacttt tttgcggtcg gaaggacgag attcgcattc ttcttcgtaa
     3121 gtggagtaaa gataggcggt ttcggtggcg aactcggcgg cgcaggtatc gacgcgtttg
     3181 taaaccggat gcagcttcag cgcgtagcgg tgttcgcgaa cttctttttc gcttacgttc
     3241 aacaattgtg ccaaacgttt gtcggagaag cctttgcgtt tcagacgacg tagggcggcg
     3301 aaatccaaat cactcaaaat gccgtctgaa accgcttttt cttccttcat caagtcttcg
     3361 atttgcgcca agaaccaagg gtcgatggcg cagatttcgt ggatttcttc cagcgtgaag
     3421 cccgcgcgga acgcgtctgc cacaaacagc atacgttcgg ggccggggtt cgccagttcg
     3481 cggcggattt ccgctttgtc ttcactgcgc ggattgaagc cgcacaagcc tgtttccaag
     3541 ccgcgcaggg ctttttggaa actttcttga atcgtgcggc ccatcgccat cacttcgccc
     3601 accgatttca tctgcgtggt caggcggtcg tctgcggcag ggaatttttc aaacgcgaaa
     3661 cgcgggattt tggtaaccac atagtcgatg gaaggctcga acgacgcggg ggttttgccg
     3721 ccggtgatgt cgttgcgcaa ctcgtccagc gtaaagccga ccgccagctt cgccgccacc
     3781 ttcgcaatcg ggaaacccgt tgctttggaa gccaacgcgg aagaacggct cacgcgcggg
     3841 ttcatctcaa tcacaatcat ctcgccgttt gcagggttca ccgcaaactg cacgttcgag
     3901 ccgcccgtgt ccacgccgat ttcgcgcaat accgccagcg aagcattacg cataatttga
     3961 tattccttgt ccgtcagcgt ttgcgccggc gcaaccgtaa tcgagtcgcc tgtatgcacg
     4021 cccatcgggt cgaagttttc aatcgagcag atgatgatgc agttgtcgtt cttatcgcgc
     4081 accacttcca tctcgtactc tttccagccg agaacggact gctcaatcaa cagctcgtgc
     4141 gtgggcgacg catcgaaacc gcgttcgcaa atcgccaaaa actcgtcttt attgtaggca
     4201 atgccgccgc ccgaaccgcc catggtgaaa gaaggacgaa tcagggtagg gaagccgacc
     4261 tgttcttgcg ccgccaaagc ttcgttcatc gtgtggcaga caaaagattt cgggcaggag
     4321 aggccgattt tctccatcgc ctccttaaag cggccacggt cttctgcttt gtcgatggcg
     4381 tcttcggtcg cgccgatcag ctcgacattg tatttcgcca gcacgccgtt gcgcgccaaa
     4441 tccagcgcac agttcagcgc agtctgacca cccatggtag gcagaatcgc gtcaggacgc
     4501 tctttggcaa taattttttc caccgtctgc cacataatcg gctcgatgta ggtaacatcc
     4561 gccatttcgg ggtcggtcat aatcgtggcg gggttggaat tcaccaaaat gactttatag
     4621 ccttcttcac gcaaggcttt gcaggcctgt gcgcccgaat agtcaaattc gcaggcctga
     4681 ccgataacga tagggccggc gccgatgata aggatggatt ttaggtcggt acgtttgggc
     4741 atggtgggtc tttcttttga agaatttgga ttattgacga ttggtaactt ctctaattat
     4801 ttctgcatct gttttttcat cgcttggatt atctgaaaag aaaaaactgt aattactatc
     4861 ggctttaaat ttttcgtata gctctgggcg caaaattttt gctttttcac tattttccac
     4921 gctcctgcga ttaggcttgc cagccttatc tttcccattt ctacggacat catagcaata
     4981 aatagtgtta ggagaattag gttctaaacg agtagctaca atctttttat aaattatgtc
     5041 taaatctttt ttagtttgtg ttggaatgaa atctgcaaaa agattactaa aactctgaaa
     5101 aatttctctg attttcgcag caccagcctg aacattaatt ttaaaaaaat taataaaatc
     5161 agtagcactt ttaaaatgaa cttcataaaa atcagcatac gcaaatattg aattttcact
     5221 attcaaatta aaaaactcta attttgcttg tgcataatca tctggtctag gttcatcagg
     5281 tttataccta gtcaaaataa cggcgtattg cctttgatta taattaaaag gaaatgcttc
     5341 aattattcta tttttgtttc tcatatcatt aagcaaatac tttaaattat tgaaattaat
     5401 aggtattgac atatttaaac tcctgcgaat atgttgcaat aaacgtagca agcgtaggtt
     5461 gggttgaaaa cccaacacaa accgcttaat tgtctaaaat gttgggtttg ctaaaaccta
     5521 acttgtatat cgaaaccccc gtatcgtagc aagccgtagc ccgtatgtag ggtatgtgcg
     5581 gtacgtatgc acgcgttctc cattttccct gcaaccccaa cccaccgcgt gcgtgccttg
     5641 cggcacactc tctgccgatg agttcaaagg tcgtctgaaa aaacgttcgc ggcgtttgtt
     5701 ccacctgcct tgcgataaag gctgcctgaa aaattcccgc tgttgatatt tcgggcattc
     5761 tttctcaaac cgcctgcaac cgcaaaatcc gctgcaacct gccccctccc ccgtggggga
     5821 gggctgggga gatggaattc tccgaatttc ggcaaccttc cccaatgcct tcaccgccta
     5881 aatacaagcc ttgcggcttg ttgccctctc tctaacgctt tcccacaggg agagaggacg
     5941 gggcggctgt tggaattaag ggttctgcag gaaaaacagg ttgtgcgggc tgttttttgt
     6001 ttttcagacg gtcttctaaa cgctcaattc aagcagcctg taccctacct gtatcctata
     6061 attttcaaaa ctctaatcaa aattaaccgt ttgttattgc gcatactgct tttccaattc
     6121 ctgcaatacg cgcaggattt ccgccagtac atcgtttgtc tgctgcaaaa tttcgtgatt
     6181 ccaaaaacgc agcacggtaa agcccaggct gttgagatat gccgtccgcg cgtggtcgta
     6241 tgcggcttgt tctgtgtgct gccctccgtc tgcttcgaca atcaacttgg gcgttacgca
     6301 cataaaatca acaatatagt ttcccatcgg ctgctggcgg cggaatttat agccgttcag
     6361 acgacctgcc cgcaggtgct gccacaattt tgcttccgcc ccgctcattt cttggcgcat
     6421 ggctttggcg cgttggcgca agatggggtt gtcggtggtc aagagtttct cgtgcgggtt
     6481 cattttgttg tcgtgctgtt tgctgccctc tctctaactc tctcccacgg ggagagagga
     6541 cggggcggtt gttggggtta aaggggctgt aaggaaaaca agtcgttcag gccacttttt
     6601 acttttcaga cgacctgcaa tcaacggttg taaatccaag ttgcttttca aaccgcctgc
     6661 aaccgcaaaa tctgctgcaa ccttctccct cccccgtggg ggagggctgg ggagagggca
     6721 ttcttcgaat ttcggcaacc ttccccaaca ccctaaccgc ccaaacacaa gccttgctgt
     6781 ttgttgccct ctctctaacg ctttcccaca gggagacagg acggggcggc tgtagtgatt
     6841 aagggttttg caaggaaaac aaggaaaaca agttgtacag gctgtttttt atttttcaag
     6901 cgacctgaaa tcaacgcttg taaatccaag tcacttttca aactgcctgc aaccgcaaaa
     6961 tctgctgcaa cctaccccct cccccgtggg ggagggctgg ggagagggca ttttccaagt
     7021 tgcggcaatc tttcccaaat cccttagtct tctaaaatac aagccttacg gttgttgcca
     7081 aatgaaactt cttccatacc caaaacttgc gtgtaaaacg cgatggttcg gtcaatgtcg
     7141 gcaacggtca gtaccagatg gtcgagtgcg ctaatcttca tttcccgctt tctttttcag
     7201 gcggcggcag tgccgtctga aagccattat gcccgttttg ccgctttcat attgccaatg
     7261 aatttgtcaa acaaatagcc gacatcttgc ggaccggggc tggcttcggg gtggccttgg
     7321 aagcagaata cgggtttgtc ggtcagctcg atgccttgca aagtgttgtc aaacaaggat
     7381 ttgtgggtaa tccttgcgtt ggcgggcagg gtgtcggcat cgacggcgaa accgtggttt
     7441 tggctggtga tgacgacttt gccgctgtcc aaatcttgca cggggtggtt cgcgccgtgg
     7501 tggctgaagc gcatttttaa ggttttcgcg ccgatagcga ggctgatgag ctggtgtccc
     7561 aagcagatgc cgaaaatcgg tttgccgctt tccatcagtt tttgtacggc tttgatggcg
     7621 taggtgcaag gctcggggtc gccggggccg ttggacagga acacgccgtc gggattgagt
     7681 gccaacacgt cttgtgcgct cgtttgggcg gggacgacgg tcaggcggca gccgcgcgag
     7741 gcgagcatac gcaggatgtt ggttttcacg ccgaaatcgt aggcgacgac gtggtaaggc
     7801 tgttcgtcag gggtaacgaa acccttgccc aattcccatt cgccttccgt ccattcgtaa
     7861 gtttccgtgc aggaaacttc ttttgccaag tctttgccga ccatactgcc gaacgcggcg
     7921 atgagttctt gcgctttttc gattgtggca tccgcaccgg tcagaatcgc gccgccttgc
     7981 gcgccttttt cgcgcaacag cgtggtcagg cggcgggtgt cgatgtcggc gatggcgacg
     8041 gtttggttgc gtaccaaata gtcgtgcagg ctttcggagg cgcggaagtt gctgtgcaag
     8101 agcggcaggt cgcggataat caggccggag gcataaacgc tgcggctttc ttcatcttcg
     8161 gcgttggtgc cggtgttgcc gatgtggggg taggtgaggg taacgatttg tttgcagtag
     8221 gacgggtcgg tcaggatttc ctgatagccg gtcatcgaag tattgaacac gacttcgccg
     8281 gaagtcgaac cttcgtaacc gattgatgtg ccgtggaata cgctgccgtc agcgaggacg
     8341 aggagggcgg gggtgctcat gatgggaatc ctgtttttaa taaaattctt gacaatgccg
     8401 tctgaaggga cttcggcagg gggcatccgg tcggaaaaat ccggtcaaaa aaaaacacgc
     8461 cgcgcaagat agacgcgtaa cgtgcttttc gggcaagtgc ctataccctg aaagcagcat
     8521 attttaagac gaaacatacc cgatttcaag cgcaaacgcg gcaggcgggc tgagatgttc
     8581 gcgcgaaaac ggaaatccgt ggcgtaaaac cgcccgctcc gcgcccacta tgcccgttac
     8641 gcatttcccc acgtccgtcc ggcgaaacta tgggaatacc cgaatcgtca ggaatacctg
     8701 aatcgtcagg aatacccgaa ccgtcattcc cgcgcaggcg ggaatc
//
LOCUS       DAWIVB010000075         8738 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWIVB010000075 DAWIVB010000000
VERSION     DAWIVB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.075"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            244..1617
                     /locus_tag="WIX60_001188"
     CDS             244..1617
                     /locus_tag="WIX60_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ5305457.1"
                     /translation="MKLIYIDPPYNTGNDGFKYNDKFNHSTWLTFMKNRLEIAKELLM
                     KDGSIFVSIDDNEQAYLKILMDEVFGNENFICNFIWEKKTGASDAKQIATITEFVLCY
                     SKNFKTVKLNKNTFSYDTERYKLSDKFEQERGKYYIDNLDRGGLQYSDSLNFAIQCPD
                     GTFTYPNGRTEFVNDGWIWKWSKNKIDWAITNGFLEFRKSKSKKSGWSVCYKNYMLVD
                     NENKPIERSAPYKNLIQDILNTHATDELKKLFGSKVFTTPKPESLLQYLIQIATSESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQE
                     PEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFADCLNDDDKALSRAFYQSVKNQ
                     AEKKDGE"
     gene            1607..4384
                     /locus_tag="WIX60_001189"
                     /pseudo
     CDS             1607..4384
                     /locus_tag="WIX60_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(4533..5705)
                     /locus_tag="WIX60_001190"
     CDS             complement(4533..5705)
                     /locus_tag="WIX60_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ5305458.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            5996..6442
                     /gene="iscR"
                     /locus_tag="WIX60_001191"
     CDS             5996..6442
                     /gene="iscR"
                     /locus_tag="WIX60_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ5305459.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6471..7685
                     /locus_tag="WIX60_001192"
     CDS             6471..7685
                     /locus_tag="WIX60_001192"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ5305460.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRVPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7949..8335
                     /gene="iscU"
                     /locus_tag="WIX60_001193"
     CDS             7949..8335
                     /gene="iscU"
                     /locus_tag="WIX60_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ5305461.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKGDDSVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            8422..>8738
                     /gene="iscA"
                     /locus_tag="WIX60_001194"
     CDS             8422..>8738
                     /gene="iscA"
                     /locus_tag="WIX60_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ5305462.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
BASE COUNT         2753 a         2003 c         1872 g         2110 t
ORIGIN      
        1 ttatacacta aattaacccg aaaaagacaa gaaatctttt ttaatcaaac ccttgctttt
       61 gatgaaattg atcggctttt tgacgcaaaa gcattctcaa aattctctcg ctataccgca
      121 gacggcaaac aagccgttgg cgaaatcaaa cgacattcag acggcacacc cgccgaaaat
      181 ctcattatca aaggcaataa tctgattgcc ctgcattcgc ttgccaagca gtttaaaggc
      241 aaagtgaagc tgatttatat tgacccgcca tataacacgg gtaatgacgg ttttaaatac
      301 aacgacaaat ttaatcattc cacttggctg acttttatga aaaaccgtct agaaatcgcc
      361 aaagagctgc ttatgaaaga cggttcgatt tttgtgtcaa ttgacgacaa cgaacaggca
      421 tatttgaaaa ttttaatgga tgaagttttc ggaaatgaaa atttcatctg caattttatt
      481 tgggaaaaaa agacaggtgc gtccgatgcc aaacagatag cgactattac agagtttgtc
      541 ttatgttact caaagaactt taaaacagtt aaattaaata aaaacacgtt ttcttatgat
      601 acagagagat acaaattaag tgataagttt gaacaggaaa gaggcaaata ttatatcgac
      661 aatttagata gagggggatt gcagtatagt gacagtttga attttgcaat ccaatgtcca
      721 gatggcactt ttacgtatcc gaatggcagg actgaatttg tcaatgatgg ctggatatgg
      781 aaatggagta aaaataaaat tgattgggca ataacaaacg gttttttgga gtttagaaaa
      841 tcaaagtcta aaaaatctgg atggagtgta tgttataaaa actatatgtt ggttgataac
      901 gaaaacaagc cgatagaacg ttctgctccc tataagaact taatacagga tatcttaaat
      961 acacatgcga cagatgaatt gaaaaaactg ttcggcagca aagtttttac tactccaaaa
     1021 cctgagagct tattgcagta tcttattcaa attgccacat ccgaatccga catcgtctta
     1081 gactaccatc ttggtagtgg cacaaccgcc gccgttgccc acaaaatgaa ccgccaatat
     1141 atcggtattg aacaaatgga ttatattgaa acgcttgctg ttgaacgctt gaaaaaagtg
     1201 attgatggcg agcaaggcgg tatttccaaa gccgtgaatt ggcaaggtgg tggcgagttt
     1261 gtttatgccg aacttgcccc atttaacgaa accgcaaaac aacaaatttt ggcttgcgaa
     1321 gattcagacg gcatcaaaac gctgtttgaa ggtttatgcg aacgctattt cttgaaatac
     1381 aacgtcagcg taaatgaatt tagtcaaatc attcaagagc ctgaatttca atctttgcca
     1441 ttagacgaac aaaaacaaat ggtgcttgaa atgttggatt taaatcaaat gtatgtttca
     1501 ttatccgaaa tggatgacga acaatttgca gattgcctga acgatgatga taaagcctta
     1561 agccgtgcat tctatcaatc agtaaaaaat caagcggaga aaaaagatgg cgaataataa
     1621 aacgttgttt gaagtgattg aaaatgaacg taaagcggtt aaaaaataca agcctgaatt
     1681 acttgaaatg ccagaattta cgtccaaaaa cttaaaatat gatttttttg aatggcaaaa
     1741 atctgccctt gaaaactttt tgatttttga ccgcacttca aagctagacg atttccctga
     1801 tttaaaaaat aagccaacgc atttgctgtt caatatggca acaggtgctg gcaaaacgat
     1861 gatgatggcg gcgttgattt tgtattattt tgaaaaaggt tatcggcatt ttctgttttt
     1921 tgtgaatcaa aacaatatcg tggataaaac ggaaaataat tttaccgatc cgacgcacgc
     1981 aaaattttta tttaccgaga agattttgca gggcgatacg gtaattccta ttcgcaaagt
     2041 ggagacattt agcccacatt cagacggcat tgaaattaaa tttaccagca ttcaaaagct
     2101 gtataacgat attcgcaccc ggcgggaaaa tcaaaccaca ttggcggatt tgcacaaatt
     2161 gaaccttgtg atgctgggtg atgaagcgca ccatttaaac gcgcaaacca aaggcaaaaa
     2221 acaaggcgaa ttagatttag aaaaggaaat gaacgaccgc accagcaatg ccgaaattga
     2281 acgtaaaggc tgggagcata tggttttgga attgttactc aataaaaatg gcaatcatag
     2341 ccaaaatgtg ctgttggaat ttaccgccac gctgcctgaa aatgccgatg tacaacaaaa
     2401 atacgctgat aaaatcatca caaaatttgg cttaaaagaa tttttgcaaa aaggttatac
     2461 caaagaaatc aatttggtat ccagtacgct gggtaagaaa gagcgagtgt tacacgcttt
     2521 attgtttgct tggtatcgac atcgaattgc gttgaaatat ggcattgcca atttcaagcc
     2581 tgtgatgttg tttagaagta agacgattga tgaatcaaaa gcggattatc tggcattttt
     2641 aaattgggca gaaaatgtgc aggcggttga tttttcgttt ttaactacat tttcaacaag
     2701 cttgaacgat agcgatagcg ataacgccaa cgaacaaggc aaaacccgca ctgaacaagc
     2761 cctaaaattt atgcaggaaa aaggcgttga gtttgcacat ttggcagatt gggtaaaaca
     2821 aaattatcaa aaacacaatg tgattattac caactccgaa accaacaaaa ccaaaaccga
     2881 aaaaaccgac agcgaaacag aaaaattgct gaataatttg gaagcggctg ataatccgat
     2941 tcgtgccatt tttacggtgg acagattaac cgaaggttgg gacgttctga atttgtttga
     3001 tattgtgcgt ttgtatgaag ggcaaaacgg cggcggttca aataaaaaat caggcaaaac
     3061 ggctgccgct actgtatcgg aaaagcagtt gattggtcgc ggcgtgcgtt attttccatt
     3121 tgcgtttgaa ggtaaacagc cgaataaacg caaatttgac aacgatatgc aacacgaatt
     3181 gcgtattttg gaagaattgt tttattacac gcacgatgag caatctcgct atattacaga
     3241 actgaaaaac gagttacgaa aagacggtta tttgcctgaa aaagacgatg ataaggtatt
     3301 ggcaacattt aaactcaaat ctgaatttgc cgataatcag gattttagag agttgttaat
     3361 ttgggcaaat aaaaaaatcc ccaatcccaa tgccagagcc aataatgcag acagcctgaa
     3421 agccaatccg caaacgcttc cattccaagt tcacggcaat caactgttgc aggaaacgca
     3481 atttacagcc gatgaaaatg atgaaatagc ccgacaaatc gacacacaaa ataattttac
     3541 tcaaatcata aaaatgagtg aaatggaacg gcacattttc aataaatccc tgcatatcaa
     3601 aggaaaaaat ggtcaatctt tattccattt tgaccgcttg caaagcaaac tcaacattta
     3661 caatcgcaat gaattgcaaa ataacttgtt aaaagattga caaattgaat ttttgggatt
     3721 agggcaagac aaacagatca gcccagatga caaacttgca ggctgcctaa aaatcttgga
     3781 aatggttgaa aaacatttga atgaaagtga tatgccattt atcggtacaa aagaatttac
     3841 gcctaaaaaa ttgtgggaaa tttttggcac accaaaacaa aaatgggtca aaaaagatga
     3901 tataaaaact gccattgcca cgcaaaatga ttggtatgtg atggataatt ttgctggaac
     3961 gagtttggaa gaagcgttaa ttcaatttat ttcagagcat ttgggcgatt tgaagtctaa
     4021 atatgatgtt catttaatcc gtaatgaaga agtgtttaaa ttgaataact tttccgatgg
     4081 tgaaggattt atgccggact ttattttatt gctgaaaaat aaacaaaaat cttcttccaa
     4141 tggtgtggat gactttttgc attaccaaat tttcattgaa ccaaaaggtg agcatttggt
     4201 ggaaaatgat tcgtggaaag acgctttttt aaaggcaatt acagcggaat acgggacgga
     4261 taagattctg caaaaagata caccgcatta tcgtttgatc ggtttgccgt tttttactga
     4321 caatcaggaa aatgaacaat ttacaaagtc attcccttta ggggcggcat cgcttgaaaa
     4381 atagagtggt gcattgcagg caaccccgtt tgacaaaact tcctttacaa aagggcgttt
     4441 tgtcagatat ttaatcaaca cattattaaa atacagccaa attttaatgc cgtccgaacc
     4501 ctgtgttcag acggcatcgt atttttcagt atctaaaccg tttccctgcc ccaatctttg
     4561 cctctcaaaa tcgaagcatc gacatcttga atatcgcggt gtcccgtaaa cgccatagat
     4621 atatccattt ctttatacag gatttccagc gcacgggtta cgccttcttc tccatacgcg
     4681 cccaagccat acaggaacgc ccgacctatc attgtacctt tcgcgcccaa agcccacgcc
     4741 ttcaaaatat cctgaccgct gcggatgccg ctgtccatcc aaacttcgat gtcgctgccc
     4801 actgcgctga cgatgtcggg caaggctttg atggcagaca cggtatcgtc gagctgtcga
     4861 ccgccgtggt tggaaacaat caatgcgtcc gcgccgcttt tcgctgcttt ttccgcgtct
     4921 tcaggttcca taatgccttt gataatcagc ttgccgcccc acaaatcttt aatgcgcgcc
     4981 acatcgtccc agctcaggcg cgggtcgaat tgttcggaag tccatgaaga cagcgaagac
     5041 aaatcgccga cgttcttcgc gtgtccgacg atattgcgga acgtgcggcg ttccgtgttc
     5101 agcattttca tacaccattc gggcttggtc gccagattga ttaaattggc gatggtcggt
     5161 ttcggcggcg cggacaggcc gtttttgatg tctttgtggc gttgccccaa aacctgcaaa
     5221 tcagcggtca ataccaatgc cgaacatttg gcatccttcg cgcgcttaat caggttttcc
     5281 ataaactcgc ggtcgcgcat cacataaagc tgaaaccaaa atggtgcgga agtgttctcg
     5341 gcaacgtctt caatcgagca gatagacatc gtggacagcg taaacggaat gccgaacttc
     5401 tccgccgccc gcgccgccaa aatttcaccg tcggcgtgtg ccatacccgt gaaacccgtc
     5461 ggcgcaatcg ccaccggcat tttcacatcc tgcccgatca ttttggtttc caggcttcgg
     5521 ccttccatat tgaccaatac tttttgacgg aagcggatgt ctttgaaatc cgaagtgttt
     5581 tcacggtagg tagtttctgt ccacgaaccc gaatcgatgt aatcgtaaaa catacgcggc
     5641 attttgcgct tggcaacgcg gcgcaagtct tcgatgcagg tcattttgct caaatcacgt
     5701 ttcatttgtc gccccctgaa tacctgaata actttatatg aaatcgataa tgtatatcaa
     5761 tattgattat aaggcaaatc atttcaacat ttgccgcatc cgccgcagct ccctacttta
     5821 agcgacataa ggtttaaaat tcaaaaataa caaattaaaa tcaaaatatt aaaaatcaat
     5881 caatctatcg atttaaacag ccaatcacac aatccgccct catacttgac tgaaacactc
     5941 agatattgga caattccacc cactaataaa aaaaccgaca tgggcaacca ccaccatgag
     6001 actgaccacc aaagggcgtt tcgccgttac cgctatgctg gatttggcga tgaacgcgca
     6061 aaccggcgcc gtcaaactca gtgccatcag cgaacgccaa aacatatccc tctcctatct
     6121 cgagcaattg ttcggcaaac tccgccgcgc cggacttgtt gaaagcctgc gcgggcccgg
     6181 cggcggctac atcctcgccg caccggcggc acgcatcaac atcgcccaaa tcatcgccgc
     6241 cgccgaagac cggctggacg caacccaatg cggcagcaaa gccaactgcc accacggcgc
     6301 gccctgcctg acgcacgatc tttgggagaa tttaaacaaa accatcaacg actacctcgg
     6361 cagcgttacc ctgcaaagca tcatcgaaca gaaaaacaac ggcgacggca gccgcgtcgt
     6421 ccaatttaca cacatccatt aaataacacc cgaaaaagaa agagcaaacc atgaccgtca
     6481 aaacccccgt ttacctcgac tacgccgcca ccacccccgt tgacaaacgc gttgccgaaa
     6541 aaatgattcc ctatctgacc gaaaccttcg gcaacccagc ctccaacagc cacagcttcg
     6601 gctgggaagc agaagaagct gtagaaaaag cacgtgcaga cattgccgcc ctgattaacg
     6661 ccgactctaa agaaatcgtt ttcaccagcg gcgcaaccga gtccaacaac ctcgctatca
     6721 aaggcgcggc gcacttctac aaatctaaag gtaatcacct catcactgta aaaaccgaac
     6781 acaaagccgt actcgacacc atgcgcgaac tcgaacgcca aggttacgaa gtaacttatc
     6841 tggacgtaca agaaaacggt ttggttgatt tagacgtact gaaagccgcc atccgcgaag
     6901 acaccatcct cgtttccgta atgtgggtaa acaacgaaat cggcgtggtt caagatattc
     6961 ctgccatcgg cgaaatctgc cgcgaacgca aaatcatttt ccacgttgac gcagcacaag
     7021 catgcggcaa agtgcctgtt gatgttgaag ccgcaaaagt tgatttgctg tctatgtccg
     7081 gccacaaagt atacggccct aaaggcatcg gcgccctgta tgtacgccgt aaaccacgcg
     7141 tccgcctcga agcccaaatg cacggcggcg gtcacgaacg cggtttccgt tccggcacat
     7201 tgccgaccca tcaaatcgtc ggcatgggtg aagccttccg cattgccaaa gaagaattgg
     7261 cacaagacac tgcacactac ctgaaactgc gcgatatttt cctcaaaggt atcgaaggca
     7321 tcgaagaagt ctatatcaac ggcgacctcg aacatcgcgt cccgaacaac ctaaacgtca
     7381 gcttcaactt cgtcgaaggc gaaagcctga ttatggcagt gaaagaactc gccgtatcca
     7441 gcggctccgc ctgcacctcc gcctcgctcg aacccagcta cgtcctgcgc gcgctcggcc
     7501 gcaacgatga actggcgcac tcatccctgc gcatcacctt cggtcgcatg accaccgaag
     7561 aagaagtgca attcgccgcc gaactgatta aatccaaaat cggcaaactg cgcgaactgt
     7621 cgccgctgtg ggaaatgttc aaagacggga ttgatttgaa ctcgattgaa tgggcagcgc
     7681 attaaagcgt accaacatgc cgtccgaacc tttagacggc attccaaaaa caaagcaatc
     7741 aagagaaaat atgaacgaac aagatttaga tttggacaat ctcgacaacc tgcttgaaga
     7801 ttttgacggc gttaccgtgg aaggcggcgt cgattcggaa aacgacgacg gctgcgaagg
     7861 cggggcgtgc aaaatctaaa cttcaggccg tctgaaaatc ggcaaacaaa ccacttaaac
     7921 catcaaaaaa cattaaggaa accacatcat ggcatacagc gataaagtaa tcgaccacta
     7981 tgaaaatccg cgcaacgtcg gcacattcga caagggagac gattccgtcg gcaccggcat
     8041 ggtcggcgcg cccgcctgcg gcgacgtcat gcgcctgcaa atcaaagtga acgacgaggg
     8101 catcatcgaa gatgcgaaat ttaaaactta cggctgcggc tcggccatcg cttcgtccag
     8161 cctgattacc gagtgggtta aaggcaaaag cctggatgac gcgctggcaa tcaaaaacag
     8221 cgaaatcgcc gaggagttgg aattgccgcc ggtaaaaatc cactgctcca tcttggctga
     8281 agatgcggta aaagcggccg ttgccgacta ccgcaaacgt caggaaaaca gataaagccc
     8341 ttcagacggc atcatcccgc aatgccgtcc gaaccacccg ccgcttcagg tccgtccggg
     8401 gcggtacaac aaggaagaaa tatgattacc cttaccgaga atgccgcaaa acacatcaat
     8461 gactatctcg ccaaacgcgg caaaggcttg ggcgtacgct tgggtgtgaa aaccagcggc
     8521 tgctcgggga tggcgtacaa ccttgaattt gtcgacgaag ccgatggcga cgacctgatt
     8581 ttcgaaggac acggcgcgcg catttatatc gatccgaaaa gcctggttta tctggatggc
     8641 acgcaagtcg attacaccaa agaaggtttg caggaaggtt tcaaatttga aaaccccaat
     8701 gtcaaagact cctgcggctg cggcgaaagc ttccacgt
//
LOCUS       DAWIVB010000076         8720 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWIVB010000076 DAWIVB010000000
VERSION     DAWIVB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.076"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            191..1726
                     /locus_tag="WIX60_001195"
     CDS             191..1726
                     /locus_tag="WIX60_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ5305463.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="WIX60_001196"
     CDS             1723..1815
                     /locus_tag="WIX60_001196"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ5305464.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WIX60_001197"
     CDS             complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WIX60_001197"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ5305465.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRINPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYHYAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3106..3276)
                     /locus_tag="WIX60_001198"
     CDS             complement(3106..3276)
                     /locus_tag="WIX60_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ5305466.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="WIX60_001199"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="WIX60_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ5305467.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GAQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            3697..4713
                     /locus_tag="WIX60_001200"
     CDS             3697..4713
                     /locus_tag="WIX60_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ5305468.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4739..5158
                     /locus_tag="WIX60_001201"
     CDS             4739..5158
                     /locus_tag="WIX60_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ5305469.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKST"
     gene            5180..5686
                     /gene="luxS"
                     /locus_tag="WIX60_001202"
     CDS             5180..5686
                     /gene="luxS"
                     /locus_tag="WIX60_001202"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ5305470.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPGKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5832..8648
                     /gene="polA"
                     /locus_tag="WIX60_001203"
     CDS             5832..8648
                     /gene="polA"
                     /locus_tag="WIX60_001203"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ5305471.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTEEGRKVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAK
                     VDGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         2039 a         2257 c         2300 g         2124 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttgcag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta ttcgttgccg tccaccaaca cctctgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggccccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tccccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcgcaaatc
     2101 ggaccgacga tgttggcggt cagcgtcgcg atgtctttgg tttcgaccgc ctcgatgtga
     2161 tgataggcat cataaagcgc cgggcgcatc aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgta tttgacaaac tcgacgcgtg tcagcagcga acctgcgttg
     2281 ccgaccaggc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaacggctt gggcatacgc gcccaaatca ggcacatttt cgtcttggta aacaatgccg
     2401 acgccgccgc ctaagtctaa atgttccaaa acaatgcctt cggcggcaag cgcgtcaacc
     2461 aaaatcaaaa tgcgctcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcata gtggtaggct
     2581 tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagacctgt ggagatgtag
     2641 ggatgggttt ttgcatcgac atcggggttg atgcgcaggg agacgggcgc ggttttaccc
     2701 aaacgtgcgg caactttctg aatacggtcg atttcgggga tgctttccat attgaagcat
     2761 ttcacgcctg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac gcctgaaaat
     2821 atggtttttg ccgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
     2881 atgtcaaaac cgctgcccag cgaggcgaag tgtttgataa tgctcagatt gccgtttgcc
     2941 ttgacggcgt aacagacgag cgggttcaaa gcggcaaacg cggtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcagcaagg
     3061 cgggggtagg ggacttgttc gcaaaatagg gtcatgtttt cgttttcatt tttgggtttg
     3121 tggagcggat tgcggtttgc tttgaagttg caaaccggtt tggattacgc cgaaacgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg agagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggcat cttaaacaaa aaacacgcaa aaagctatga tgaccgaaag
     3361 cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa
     3421 cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag ccggagacgg
     3481 cgcccaaatc atcgtcaacc gccacacgcc caatcaggaa ttgtggattg ccgcaaaaag
     3541 cggcggctac catttcgccg agcaaaacgg caaatggctg gcaacgcgcg acggacgcga
     3601 tttttatgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg tggaaattgc
     3661 ggaattgtga tttgggtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
     3721 ggactgatat ttatcgcgat tatcgctata cttgctaact atttaggaaa cactgatttt
     3781 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
     3841 aataccattt atccgcaatt ttcgacacaa gtggaaaaag gcgttttgtt tgccaaaggc
     3901 gcgcttcttc gcactggcat tgtgttgtat ggttttcgcc tcacttttgg cgatattgcc
     3961 gatgtaggat taaatgcggt tgtcactgat gcaatcatgc taatttcaac cttctttttt
     4021 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
     4081 gcaggttgca gcatttgcgg tgcggcagca gtgatggcgg cagagcctgt tactaaagca
     4141 gaatcccata aagtttcagt ggcgattgcc gtagtggtca ttttcgggac gcttgctatt
     4201 tttacttacc ccttgttcta cacgtggtca caacatttaa ttaacgccca tcaattcggt
     4261 atttatgttg gttctagtgt acacgaagtg gctcaagtgt atgcgattgg ggaaaatatt
     4321 gatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
     4381 ccctttttat taatgctttc ttggttatta acacgtagta atggagtatc agaaaataca
     4441 tcacacaaaa ttacaattcc ttggtttgct gtacttttta ttggtgttgc catttttaat
     4501 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
     4561 ttaatttcat caatggctgc gcttggctta acgacgcaag caagcgcaat caaaaaggca
     4621 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
     4681 ttagtgaact atggaatatc aaaattaata taaaattcac taaagagagc gttacccaat
     4741 ggcacaatta ccgctatatc tgacttctga aatcaaagac tttactgtcg gcacgcctaa
     4801 agttttagaa tcattttcca aacatatccc ttatggtgtc gtctttgaag acgacggcga
     4861 cacaggctac ttctatgccg cttcgcaaga cgggatttta gatgccttgc acatctataa
     4921 tgtcgaagat gtatccgaca aacatatccc caatcatgtc ttgattttat gggatgatgc
     4981 ctgcaccata gccgcattgt gtatcaacga ctacattcat gccgtctatg attttgtcga
     5041 acaggcagga tattgccgca acggcttccc tgaagcaggc ggcgaatggg tgaaagtcga
     5101 aaaccgcgtc ttggatgatg aattgctgga caaaatccta tcccgaaaat ctacataacc
     5161 ctcacaaaag gatacccaaa tgcccctact agacagtttc aaagtcgatc acacccgtat
     5221 gcatgccccc gccgtacgcg tggcgaaaac catgactacg cccaaaggcg acaccattac
     5281 cgtgtttgac ctgcgctttt gcgttcccaa caaagaaatc ctgcctggaa aaggcataca
     5341 cacgctggag catttgttcg caggttttat gcgcgaccac ttgaacggca acggcgtgga
     5401 aatcatcgac atttccccga tgggctgccg caccggtttt tatatgagtc ttatcggcac
     5461 gccttccgaa cagcaggtcg ccgatgcgtg gctggcttcg atgcaggatg ttttgaatgt
     5521 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
     5581 ctcgctcgcc gaagcgcagc aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa
     5641 caaaaatgaa gagctgacgc tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc
     5701 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatccgg ggttgtccgg
     5761 gggcgggttt taagccggca tcgtccttcc ctattttttt ctgtccctta tcggttttaa
     5821 gcgggttttt tatgtccaac agacctacac tcctcctcgt tgacggatcg tcctacctct
     5881 accgtgcgta tcacgcgatg gggcaaaacc tgaccgcccc cgacggcgcg ccgacgggtg
     5941 cgctgtatgg tgtattgaat atgttgcgcc gtttgcggtc ggaatatccg cacgattatt
     6001 gcgcggtggt ttttgatgcg aaaggcaaaa atttccgcca tcaaatgttt gaagaataca
     6061 aggcgacgcg cccgccgatg cccgacgatt tgcgcccgca ggcggaagca ctgccggatt
     6121 tagtgcgcct gacaggctgg ccggtattgg tgattgggca ggtggaggcg gacgatgtga
     6181 tcggcacgct ggcgaaacag ggggcggaac atggtttgcg agtcattgtt tcgaccggcg
     6241 ataaggacat ggcgcagttg gtggatgagc gcgttacgct ggtgaacacg atgagcagcg
     6301 aaacgctgga cattgaaggc gtgaaggcaa aattcggcgt gcgccccgac caaatccgcg
     6361 attatctcgc gctgatgggc gacaaggtgg acaacgtgcc gggcgtggaa aaatgcggcc
     6421 cgaaaacggc ggtgaaatgg ctggaagcct acggttcgct ggctggtgtg atggaacacg
     6481 cttcggaaat caagggcaaa gtgggcgaaa acctgcaagc cgcgctgccc caactgccgc
     6541 tgtcgtatga tttggtcacg attaaaaccg atgtggactt gcacgccgag ctttcagacg
     6601 gcatcgaaag cctgcgccgt actacgccga aatgggcgca gctggttgtc gatttcaaac
     6661 gctggggctt ccgcacctgg ctgaaagaag cggaatcaaa catgaatacc ggctcgaccg
     6721 atgatttgtt cggcagcgac agcatcggcg agcaggcggc tttgaatgcg gaaatgccgt
     6781 ttgaaaaaca agccgaaaaa gccaccgccc ccgaaaaact ggattatcaa gccgttacca
     6841 ccgaagcgca gtttgccgct ttgttggaca aactgtcgcg ggcggacaca atcggcatcg
     6901 atacggaaac cacgtcatta gacgcgatga acgcctcgct ggtcggcatc agcatcgctt
     6961 tccaagcagg cgaagcggtt tacatccccg taggacacag cctgaccgcc gcgcctgaac
     7021 agcttgattt acaagacgta ttaggccgtc tgaaaccgca tttgggaaac cccgccctaa
     7081 aaaaaatcgg gcaaaacctc aaatacgacc aacacgtttt cgccaactac ggcatcgccc
     7141 tgaacggcat tgccggcgac gccatgctcg cttcctacat catcgagagc catctcggac
     7201 acggcttgga cgaattgtcc gaacgctggc tcggcttgga aaccattacc tacgaatcgc
     7261 tgtgcggcaa aggcgcgaag caaatcggtt ttgccgatgt cgccatcggg caggcgaccg
     7321 aatacgccgc ccaagacgcc gatttcgccc tgcgcctcga agcgcacctg cgcgcgcaaa
     7381 tggacgaaaa acagcttgaa atgtatgaaa aaatggagct gcccgtcgcg caggtattgt
     7441 ttgaaatgga acgcaacggc gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg
     7501 aactcggcgc cgagctgatg aagctcgaac aggaagccta tgccgccgca ggccagccgt
     7561 tcaacctcaa ttcgcccaaa cagctgcaag aaatcctgtt cgacaaaatg ggcatcccca
     7621 ccaaaggcct gaaaaaaacc gccaaaggcg gcatttccac caacgaagcc gtgctcgaac
     7681 agctcgcgcc cgactacccc ctgcctaaaa tcatcctgca aaaccgcagc ctggcgaagc
     7741 tcaaatccac ctacaccgac aaactacccg aaatgatttc ccccaaggac ggccgcgtgc
     7801 ataccaccta cgcccaagcc gtcgccatta ccggccgcct cgccagcaac aaccccaacc
     7861 tgcaaaatat ccccatccgt accgaagaag ggcgtaaagt ccgccgcgcc tttaccgcac
     7921 cgcaaggcag cgtcatcgtt tccgccgact attcccaaat cgagctgcgc attatggcgc
     7981 acctctccgg cgacaaaacc ctgattgccg cgttccaaaa cggcgaagac gtacaccgcc
     8041 gcaccgccgc cgaagtgttc ggcactgcgc ccgaaaacgt ctcgtccgag caacgccgct
     8101 atgccaaaac catcaacttc ggcttaattt acggtatggg gcaatacggt ttggcaaaat
     8161 cattgggcat cgacaacctt tccgccaaaa actttatcga ccgctacttc gcccgctacc
     8221 ccggcgtcgc cgaatacatg cagcgcacca aagaacaagc cgccgcccaa ggctacgtcg
     8281 aaaccctgtt cggcagaagg ctctacctgc ccgacatccg caacaaaaac gccaacgccc
     8341 gcgccggagc cgaacgcgct gccatcaacg cccccatgca gggcaccgcc tccgacctca
     8401 tcaaacgcgc catgatagac gtgtcccgct ggctttcaga gtgcgaagcc tccccgtggg
     8461 acgaactctt acaaagcaaa ctgattatgc aggtgcatga cgaactggtg ctggaagtcg
     8521 ttgaaaccga actggatttt gtcaaagaaa aactgccgca gattatggcg aaagtggacg
     8581 gcggattatt ggatgtaccg ctggtggctg aggttggcgt aggggagaat tgggaagagg
     8641 cacattgatt gaaaggtgtt atatgctatc tttatttaaa taaaatttaa tttttggtat
     8701 attttttcta aatgttccta
//
LOCUS       DAWIVB010000077         8598 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWIVB010000077 DAWIVB010000000
VERSION     DAWIVB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.077"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(391..825)
                     /locus_tag="WIX60_001204"
     CDS             complement(391..825)
                     /locus_tag="WIX60_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ5305472.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WIX60_001205"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WIX60_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ5305473.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WIX60_001206"
     CDS             complement(1556..2398)
                     /locus_tag="WIX60_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ5305474.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WIX60_001207"
     CDS             2653..3291
                     /locus_tag="WIX60_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ5305475.1"
                     /translation="MFQNFDLGVFLLAVLPVLLSITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WIX60_001208"
     CDS             complement(3743..4543)
                     /locus_tag="WIX60_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ5305476.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4679..5182)
                     /locus_tag="WIX60_001209"
     CDS             complement(4679..5182)
                     /locus_tag="WIX60_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305477.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5519..6160)
                     /locus_tag="WIX60_001210"
     CDS             complement(5519..6160)
                     /locus_tag="WIX60_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ5305478.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
     gene            6303..8516
                     /locus_tag="WIX60_001211"
     CDS             6303..8516
                     /locus_tag="WIX60_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ5305479.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWLLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQKPE
                     KYREIALLLDEKRTERLEYIENFLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIIGFVTR
                     ERGISVHRKTCPSFQHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT         2264 a         2339 c         1870 g         2125 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggtgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccaatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 cgtcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt ggtaccagtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgca gcaggcgcgc
     2221 ctgcaccttc gggggcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctct ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttca cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatcta gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg cggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctactg ctgatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaggtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt gaccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcaggcaaaa taaggcatcc accccccttc ttcattaagg atatatattg
     4801 agaaacaaat cgcaactaaa cagaaaaaac ttgggagata aagccatttc attcccctat
     4861 tcaagaatct agccaagata ggtattttgt attctacaaa aaagaaaggc atttccaagg
     4921 gaaacatgtc agataaaaac ttttgtttat tttttactat agatagaacc ttgcttctca
     4981 agagaaagcc attaataata ccgatgacag ctattaatat atagagaata gtataagtat
     5041 gaataatctt cattagacaa aaagaagaaa tggcagataa attacatacg atatattgga
     5101 atataaaata tttacggtct aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca
     5161 taaaaaaatc aaaggcgtcc attaaactat ctttcacatt agaaatttaa aagctaaata
     5221 atacgacaaa caatgtgaag tactattcat ggtttattta aaaaataata ctattctgaa
     5281 cattatttag atacagaaat taacaaatta gaactaaaca agcttttaaa tactttaatt
     5341 ttattggaaa gctataaaag gaactataac tttacacact agtcacttct ttttaagagg
     5401 caaaagggat tgggaaggtc gtcttggaga taagcactgg tatttcggcc aatggtaaat
     5461 agagtttacc tcaaataggg tagaacctcc ttcatctgtc agttaataac agccactttt
     5521 acaatgccct gtcaaaataa agcggcacgc ccgatttttc actcatcgtc atcaaataac
     5581 ccatcacctt ttggggccat tcgatgccgc gcaccacggt cagattcctc aaaacgggga
     5641 aaaccaaaat atcctccata ccgattccgc cgttgatgcc gtctgaagca ccgtccatca
     5701 aattttccaa ctcttgcaaa tctgcgttta tccgttcgag gtattgggcg gttttattca
     5761 aattggcgga aaagctgccg atgcttttct cttttttgtc tgtaaaatat ttcaccgctt
     5821 ccggcgttgc aaattcaggc agcccgattt tgatcacgcg cggctgcacc agtttgtcgt
     5881 tgtatccgcc caccttgtcc agccacgccc gtatctcggg gcggacttcg tctttcagac
     5941 ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc gcccataaac gaaccgtctt
     6001 ctttttgcag gacgggcact tgtttcgcac cgatcatacc gatcggcgtt gcctcgtcgt
     6061 cgtttgccag cacggcttct tcaacgtccg cgccaaacag cccggcagcc atccgcgcac
     6121 gcacgcaaaa cgggcaatgg tcgtaaatat acagtttcat caaaatattc ctcgtcaacc
     6181 tgtcggtacc gactacctta acaccccgcg ccgcccgaaa caagtttatc ttcccgccta
     6241 tgcaccgtaa ataaataagc tgttacaata aactcgtttt tatcggaacg gaagacccca
     6301 tcatgaccgc catcagcccg attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg
     6361 aatggttcga cagctactgc gccgctctgc cggacaacga taaaaacctc atcggtaccg
     6421 catggttgct ggcgcaggaa cattaccccg ccgatgccgc cacgccgtat ggcgagccgc
     6481 tgcccgacca cttcctcggc gcggcgcaaa tggttcatga actcgacctg ctccccgatg
     6541 ccgtcgccgc caccctgctt gccgacatcg gacgctacgt ccccgactgg aacctattgg
     6601 tttccgaacg ctgcaacagt accgtcgccg agctggtcaa aggtgtggac gaagtgcaga
     6661 aactcaccca cttcgcccgg gtggacagcc tcgccacgcc ggaagaacgc gcccagcagg
     6721 cagaaactat gcggaaaatg ctgctggcga tggttaccga catccgcgtc gtgttaatca
     6781 aactggcgat gcgtacgcgc accctgcaat ttttaagcaa cgcccccgac agccccgaaa
     6841 aacgcgccgt cgccaaagaa accctcgaca tcttcgcccc gctcgccaac cgtttgggcg
     6901 tgtggcagct caaatggcag ctcgaagatt tgggcttccg ccatcaaaag cccgaaaaat
     6961 accgcgaaat cgcgctgctt ttggacgaaa aacgcaccga acgcctcgaa tacatcgaaa
     7021 acttcctcaa catcctgcgc ggtgaactca agaaatacaa tgtccatttc gaagtcgccg
     7081 gccgcccgaa acacatctac tccatttaca aaaaaatggt gaagaaaaaa ctcagcttcg
     7141 acggcctctt tgacatccgc gccgtgcgaa ttctggttga taccgtcccc gagtgttaca
     7201 ccacgctggg tatcgtccac agcctctggc agcccattcc cggcgagttc gacgactaca
     7261 tcgccaatcc caaaggcaac ggctataaaa gtttgcacac cgtcatcgtc ggcccggaag
     7321 acaaaggcgt ggaagtacaa atccgcacct tcgatatgca ccaattcaac gaattcggtg
     7381 tcgccgccca ctggcgttac aaagagggcg gcaagggcga ttccgcctac gaacagaaaa
     7441 tcgcctggtt gcgccaactc ttggactggc gcgaaaacat ggcggaaagc ggcaaggaag
     7501 acctcgccgc cgccttcaaa accgagcttt tcaacgacac gatttatgtt ttgaccccgc
     7561 acggcaaagt cctctccctg cccacgggcg cgacccccat cgacttcgcc tacgccctgc
     7621 acagcagcat cggcgaccgt tgccgcggtg cgaaagtcga agggcagatt gtgccgctgt
     7681 ccaccccgct cgaaaacgga cagcgcgtcg aaatcattac cgccaaagaa gggcatcctt
     7741 ccgtcaactg gctttacgaa ggctgggtca aatccaacaa ggcaatcggc aaaatccgcg
     7801 cctacatccg ccagcaaaac gccgacaccg tgcgcgaaga aggccgcgtc caactcgaca
     7861 aacagcttgc caaactcacg cccaaaccca acctgcaaga gcttgccgaa aatctcggct
     7921 acaaaaagcc agaagacctc tacaccgccg tcggacaagg cgaaatttcc aaccgcgcca
     7981 tccaaaaagc ctgcggcacg ctgaacgaac cgccgcccgt acccgtcagc gaaaccacca
     8041 tcgtcaaaca gtccaaaatc aaaaaaggcg gcaaaaacgg cgtgctcatc gacggcgaag
     8101 acggtctgat gaccacgctt gccaaatgct gcaaacccgc gccgcccgac gatattatcg
     8161 gcttcgttac ccgcgagcgc ggcatttcag tgcaccgcaa aacctgcccg tctttccaac
     8221 acctcgccga acacgcgccc gaaaaagtgc tggacgcaag ctgggcggca ttgcaggaag
     8281 gacaagtatt cgccgtcgat atcgaaatcc gcgcccaaga ccgctccggg cttttgcgcg
     8341 acgtatccga cgcgctcgcc cgccacaaac tcaacgttac cgccgtgcaa acccagtccc
     8401 gcgacttgga agccagcatg aggttcacgc tcgaagtcaa acaagtcaac gacctcccgc
     8461 gcgtcctcgc cagcctcggc gacgtcaaag gcgtattgag cgttacccgg ctttaaatac
     8521 aaaaatgccg tctgaaagcc gaataacgct tcagacggca ttttgattgc cggggtttgc
     8581 tattttttgt tgcatagt
//
LOCUS       DAWIVB010000078         8592 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWIVB010000078 DAWIVB010000000
VERSION     DAWIVB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.078"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(170..382)
                     /locus_tag="WIX60_001212"
     CDS             complement(170..382)
                     /locus_tag="WIX60_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5305480.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(394..675)
                     /locus_tag="WIX60_001213"
     CDS             complement(394..675)
                     /locus_tag="WIX60_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305481.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGRHYRHSENGITLYDFIFFDGKPADEARFAAILTETCRRAVKKICETK"
     gene            complement(713..976)
                     /locus_tag="WIX60_001214"
     CDS             complement(713..976)
                     /locus_tag="WIX60_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305482.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMIAATGLVTAIAAVLALIFKFA"
     gene            complement(996..1568)
                     /locus_tag="WIX60_001215"
     CDS             complement(996..1568)
                     /locus_tag="WIX60_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEZ5305483.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVL
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1547..1858
                     /locus_tag="WIX60_001216"
     CDS             1547..1858
                     /locus_tag="WIX60_001216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305484.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1880..2755)
                     /locus_tag="WIX60_001217"
     CDS             complement(1880..2755)
                     /locus_tag="WIX60_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ5305485.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2800..3090)
                     /locus_tag="WIX60_001218"
     CDS             complement(2800..3090)
                     /locus_tag="WIX60_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ5305486.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3102..3347)
                     /locus_tag="WIX60_001219"
     CDS             complement(3102..3347)
                     /locus_tag="WIX60_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ5305487.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAKNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGHEIAKKLGLK"
     gene            complement(3340..3564)
                     /locus_tag="WIX60_001220"
     CDS             complement(3340..3564)
                     /locus_tag="WIX60_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ5305488.1"
                     /translation="MEKPLNFKPISYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            3836..4207
                     /locus_tag="WIX60_001221"
     CDS             3836..4207
                     /locus_tag="WIX60_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5305489.1"
                     /translation="MNNSSFFGNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGIS
                     QPKAELFFQFEKVGIDVQYVMHGRRGETAVMPSETLNAEEQELLVLFREAAAADREMI
                     LMVARRAEKKAQTALGKVSNG"
     gene            4209..4832
                     /locus_tag="WIX60_001222"
     CDS             4209..4832
                     /locus_tag="WIX60_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305490.1"
                     /translation="MDQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLWARS
                     AKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSIYDL
                     LGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKKFVM
                     PCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            4825..5019
                     /locus_tag="WIX60_001223"
     CDS             4825..5019
                     /locus_tag="WIX60_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5305491.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            5043..5606
                     /locus_tag="WIX60_001224"
     CDS             5043..5606
                     /locus_tag="WIX60_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305492.1"
                     /translation="MKILALLIAATCALSACGSQSEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSAANKGLNDQKTGLTLPEHVVPIDNAEGKNLLHDFSDGLTILTVDTDKADKIT
                     AVRVVWNTDAMPQKAEKLSKAAAALIAATAPEDRTMLRDTGDQIEMAIDSHNAQKEPT
                     REWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            5637..6584
                     /locus_tag="WIX60_001225"
     CDS             5637..6584
                     /locus_tag="WIX60_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305493.1"
                     /translation="MMIEKSISIVDGKEYSVFAVSHEFRYTFDEPILVADLISSLKAY
                     ETLTSSYLPAILNQLFDVKIQKIKVAVSEIERGSFLEKLIFNLFFKDEDAYNEFCLKI
                     RKFLGTENQDGSINMSKIIMFAMTTLLGVGAGYLLFKNPPQEKQAITNNIVTVINADS
                     SVALDGEHLVSVVKEVTGSSKQKTAENVAKVYAPASKNNGSITLGTDDVRIEPVAQQT
                     VATLPKDVDLRDTPLTEDYTDIDVQIRATDRDKNSGWYAVIDQIVPSRVRLELPEDID
                     LNRLANNATIRANVTVEFDLKQNGSRKPKKIILTSLSTD"
     gene            6669..7172
                     /locus_tag="WIX60_001226"
     CDS             6669..7172
                     /locus_tag="WIX60_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ5305494.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGVIGAVSLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            7284..7616
                     /locus_tag="WIX60_001227"
                     /pseudo
     CDS             7284..7616
                     /locus_tag="WIX60_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7613..8023
                     /locus_tag="WIX60_001228"
     CDS             7613..8023
                     /locus_tag="WIX60_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ5305495.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            8056..8589
                     /locus_tag="WIX60_001229"
     CDS             8056..8589
                     /locus_tag="WIX60_001229"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ5305496.1"
                     /translation="MSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRRIAAAIR
                     HAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEKPNEKWV
                     TDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPGSSEKPI
                     LHSDQGWQYQMFFIKSS"
BASE COUNT         2134 a         2052 c         2160 g         2246 t
ORIGIN      
        1 gaaaacagct gaaaacctgt gtttgggttt cggctgtcgg gagggaaagg aattttgcaa
       61 aggtctcaga gtgagtttat tttggggcgg cggcaggtcg ggggcaagcg gcgtggggct
      121 tggttgtggt ttttaggttt ttgggggtaa aaaatgccgt ctgaactttt cagacggcgt
      181 ttgttttttc tatccaatcg aggaactgcc gccatttttc cagcggcata tcggcccgac
      241 gggtttcggt atcgggttcg gcttcccagc ggcctacctg tatgtagtgc ttcaccccga
      301 cgatttgcgc caactcggcc tgtgtcagtt tgcagcggtt gcgaagggtg cggaggttgt
      361 aaggcgtgta gccgagttcc atgtcgttgc ggtttatttt gtttcgcata tttttttgac
      421 tgcccggcgg caggtttcgg taaggatggc ggcaaatcgg gcttcgtctg ccggtttgcc
      481 gtcgaaaaaa ataaaatcgt aaagggtgat gccgttttcg gaatggcggt aatgcctgcc
      541 cgggcattcg ccgtctgtct gcctgtggat tttggcaatc aggcggggat agcggcaatg
      601 ggtaatgaaa cggcttgcgc cgtcttgccc gataatccat tcggggtagc ggttgaatag
      661 tgcggctctg ttcattttgt tcgtgggata aagcccctcg cggggcttgt ggtcaggcaa
      721 atttgaatat cagtgccaac acggcggcga tggcggtaac cagcccggtg gccgctatca
      781 tgggatacca gcgggactct tgggctattt ttacggattc ggcgttgatt ttgtgcgcgt
      841 cggcaatgat tttggcgatt tcggcatcta tttttctcag actggagtgt ttcatttctt
      901 gttcgattat gttcatcgta cttcctttcg tttttggcgg ttgccgccgc ttgtcggatg
      961 gtaggatgtc tgccatgtgt atatattgat accttttagg ttttattgca agtgttttgg
     1021 gcggcggctt cgtatgcttg gcggtggcgg cggctgtacc attcggcgag ttcgcgccgc
     1081 tcttggaggc ggtagctgtc ggcggtcagg tagtggtgca ggcttgagaa gccggcgcgt
     1141 tggagggcgg cgcggctgat gtggccgagg gcgaggtctg ttgtgagggt gtcttttccg
     1201 gcggtcaggc tgatgttggt gaagaggtgg cggaatccgt gcattgtgtg tttggatttg
     1261 ccgggggtgc tgccgtcata gcccagtcgt cggatggcgt tgtgggcgaa tttgatgctg
     1321 atgtggtcgg gatggggggc gggtttgcgc cgtgggcgga tgccggggaa aaggtggatg
     1381 ttgtcgccgg tctgtgtgtg cagctctcgg agtatttcta ccgcccagtc cgacagtagg
     1441 acggtaaagg ggtgtttggt cttcatgtcg gcggcgggga tgtgccataa ccgggcggtg
     1501 aggtcgatgt cttgccagcg ggcggaaagc agtgcggacg gacgggatga cttccccggt
     1561 ttccccatca atccgcgccg cctcgatttt tccgttggcg cgcagcattt ccaccgcctg
     1621 ccgcaccgac atcaaccgct tgccgttgcg cctcatcgcc tccaccaaaa ccgccgaaat
     1681 caatttggct tcttccggtt taagctccgt cttgcccgca tcgctgcgcc gtttgcgcgt
     1741 cggcttgacg ctgaccgcct ccagcttgcg gtatagcgtg gcaaggctga tgcccaattc
     1801 ctgcgcctgc tgcttaagat atgcagagcg tgcgccgcgt tccattgctt ccgcctgatt
     1861 ctcgactgcc ttaagacgct caatcattgc cggattcatc gccttctccc gtttcaccgc
     1921 ccaaccattc cggcacattg tctgtcggtg cttcagtcgg cagggcatag ctttcgcgca
     1981 gttgctcgca gtccaaaata atttgattga gcgtgccgac catctttgcc tgatggctga
     2041 tcccgtgtgc ctcactgtgc gcattaagtt ggtcgaacaa atctttcaga cggctcactt
     2101 gactgcggat accgacctca aggcttgtta actgcatcgc caactcgctg cctacgtctt
     2161 ccgccttcgg ctctctgaca acggtttgct tcttagccag cttctcggcc agctcatcaa
     2221 ttttggctgt tttggttttc atcacttcgt ctttggcggc gaggttttcg cggctttcgc
     2281 gcagggcgac gcgcagctcg cgcaccgtca ttcggtccac atcgtcaaag gtcatgccgt
     2341 tgacttcttc cccttcggcc aaacccacca gcgtaacgtc ttcttcgacc agcagctcaa
     2401 gcagcttcga cttgcccaaa tccatcagct tcggcgcggc tttctgcatt tgcggggtcg
     2461 caaagcggcg agtggctgac atcagacgtg atgtttctgc gatacccagc ccaaattggc
     2521 ttttcacaat ttccataaac cgtccgtgtt ccgtatgctc ttttaaaata atcagcgcac
     2581 gtcccagttc gaacatgcct tccatcgtct gccgtactgc ttgacgaccg cgttcaaccc
     2641 aacgctcttc gctataagtc tcgccgttac cccactgctc catcaccagc acactgcgca
     2701 tcgctgcctg attgcttact ttgtcggttg cgataatttc aatttctgta ttcatttttt
     2761 atctccaaag tttccgacgt cggaaacttt caaaatccgt taatccacat cgacccgctt
     2821 gccgatttcg gcaatcttgc tttgcagccg ttcatgctgc tgcctgaacc gctctgcgat
     2881 ttgcagggtt ttgatgccgt aggcgtagtt gccgttttca agtttgatga ccaatcccga
     2941 ggcaaccaaa tcatcaatat ccctgctgac ttgcgatggc gtcagcccca gtccgaccga
     3001 taaatcctta ttgctcagac cgataatcgg atgctcgtca agcgcgataa agaccctcaa
     3061 tagccgttgt acccttttac tttctgccat ccgcatcctc cttatttcag tcccagcttc
     3121 ttggcaattt cgtgcccctt gccgtaattg cctttgcgct gtccgccgat caccagatac
     3181 acatcgcgcg gcttaaagcc gttctctctt gcccactgcg ccagcgtctg gccgtttttg
     3241 gcaaaatttt ctttcaattc gtctgccgta agcatttctt tggcctgtac gcgggaaaat
     3301 ttcagtatcc gtccgtacgg ttggttaata cactcatgat taatcatcgc ttttctcctt
     3361 catccccagc aaaaccgccg cttcgtggct tttgccaaaa ttgcctttca gcttgccgcg
     3421 caagaggtgc tccaccgtgg tgcgctccag attgaaatat ttcgcccaat gcgccttgca
     3481 caccccgttg cgcttaaacc acccggccgc gctctcgcgc gtttgcggat aggaaatagg
     3541 cttgaaattc aaaggttttt ccatattatt tatgcctttc gtgtgatata atgtgtttgt
     3601 ttttaggtct ctttgcacga taaacatccg gtctgttgtg cagcagcagg gtcttggctt
     3661 gttttaaagt aaacaagctt tttctcgtag ctgggtgtat gtgatccatc atgagctgtc
     3721 gcgcatgagc cttcaaagtg ttcatttttt cgtcctttct cgtgatgatt tagggtgttt
     3781 gtgtttcgat gtggaaatta taggaagaat tcttcctatt ttgcaaggaa tatttatgaa
     3841 taactcttcc ttttttggca accgattgaa agaagaaaga aaaaaattaa aaatgactca
     3901 agctgaaatc gctgaaaaat gtggggtttc aggaagaatg tggggggatt atgaacgtgg
     3961 catcagccag ccaaaagcgg aacttttctt ccaatttgaa aaggtgggta tagacgttca
     4021 atacgtcatg cacggcagac gcggcgaaac agcggtcatg ccgtctgaaa ccctgaacgc
     4081 cgaagaacaa gaactgctgg tcttgttccg cgaggcggca gctgccgacc gtgaaatgat
     4141 tctgatggtt gcgcgcaggg cagagaaaaa agcccaaact gcgcttggta aagtgagtaa
     4201 tggataaaat ggatcaattc gaattgtgcc aaaaagaaca tgtcaatcca tttgccttgt
     4261 caaagcaata tctattggtt gtaacattcg tcaaatccag cagtaaaaat tttcaggcag
     4321 cattactttg ggcaagaagt gccaaattat ttgagaatct tgagattgga aaagaaacca
     4381 tctattgctg cgctttcgat aaaacagcag aacaggctgg gatggccggg gtatttttga
     4441 attatattga aaattggaat ggcaaacaga tttacatcaa tggccgaatc catagtggca
     4501 gtatttatga tttgttaggg gttttagact gctatcaaaa atcacagtcc tgtcccaacc
     4561 ctaaaagcca ctgttgcttt gtttcagacg acatttttct atggcatgga tcaagaccaa
     4621 cgtttgaaat cagtcttgat ctaactggaa agaaaaaaga aacatcctct gcaaagaaat
     4681 ttgtgatgcc ttgtattaat ttccgtcacc ataggattga aaaagaaacc tacttaggaa
     4741 attggaatga acaaattgcc gcattggcag taaaacaaaa tatagattgg tgtccaagtt
     4801 ttgatattga gaattttaga cagtatgaat aattactatc tatataggaa ttgcagcagc
     4861 gatgtgttat gggtcaaacg tatccaacgc caaatcgacg gcagcctact cttgatttct
     4921 gacaattcaa cctatccacc catgcccttg gcactggcgg aacaccccga tattcaaatc
     4981 atcgggcagg tagtgcaggt atcaaaagac ttgaactaga cacaatcaaa aagggaaata
     5041 gaatgaaaat actcgcttta ttaattgccg ctacctgtgc tttatctgcg tgtggcagcc
     5101 aatctgaaga acaaccggca tctgcacaac cccaagagca ggcacaatcc gaattaaaaa
     5161 ccatgccggt aagctatacc gactatcaat cagcagccaa taaagggctg aatgaccaaa
     5221 aaaccggtct gacccttcct gaacatgttg tccctatcga caatgcggaa ggaaagaatc
     5281 tgctgcatga cttttcagac ggcctcacaa tcttaaccgt tgataccgat aaagccgaca
     5341 aaattactgc tgtccgagta gtctggaata cagatgcaat gcctcaaaaa gcggaaaaac
     5401 tgtccaaagc tgccgcagcc ttgattgcgg caaccgctcc ggaagaccgc acaatgctgc
     5461 gtgataccgg cgaccaaatc gaaatggcga ttgacagcca taatgcgcaa aaagagccaa
     5521 cccgagaatg ggcgcgtggt gggattgctt ataaagtcac tgttaccaat ttaccgagcg
     5581 tggttttgac ggcaaaagct gagtaaatct attaagtaga aaaaatagaa agggaaatga
     5641 tgattgagaa aagtatttct attgtagatg gaaaggaata ctccgttttt gctgtatcac
     5701 acgagtttcg ttataccttt gatgagccta ttttagtcgc tgacttgatt agttctctaa
     5761 aagcttatga aacactgaca agtagttatc ttccagcaat tttgaatcag ctgtttgatg
     5821 tcaaaatcca aaaaatcaaa gtagctgtat ctgaaattga aagaggatct ttccttgaaa
     5881 aactgatttt caatttattc ttcaaagatg aagatgctta taatgaattt tgtcttaaaa
     5941 tacgaaaatt tctaggaaca gaaaatcagg acggaagtat taatatgtcc aaaatcatta
     6001 tgtttgcaat gactacactt ttaggggtag gtgctggtta tctcttgttt aaaaacccgc
     6061 cacaagagaa gcaggcaata accaacaaca tcgttaccgt cattaatgct gatagttctg
     6121 tcgcactgga tggtgaacat ttggtttcag tggtaaaaga agtaacagga agcagcaagc
     6181 aaaaaactgc agaaaatgtg gcaaaagtat atgctccagc aagtaaaaat aatggcagta
     6241 ttacccttgg gacagatgat gttcggattg aacctgttgc acaacaaact gtagcaactt
     6301 tgcctaaaga tgtggactta cgtgatacgc cattgactga agattacacc gatattgatg
     6361 tgcaaattcg tgctactgac cgtgataaaa attcagggtg gtatgcagtc atagaccaaa
     6421 ttgttccatc acgtgttcga ttagaactgc ctgaagatat tgatttgaat aggctggcta
     6481 acaatgctac tatccgtgca aatgtaacag ttgagtttga cttaaagcaa aatggctctc
     6541 gtaagcctaa aaaaatcatc ctcacatctc tctctactga ttaagtttta acccgtatta
     6601 aaggcttagt cagacggcct ttcctacaat ccctgtattg atttttaatt caatacaggg
     6661 atttttccat gtcagacaag ttcaaccaat tcatcaaccg cgtcctctct cacgagggtg
     6721 gttacgccaa ccatcccaaa gaccccggcg gcgaaaccaa ttggggcatc actaagcgca
     6781 ccgcacaggc aaacggctac aacggctcca tgcgtgccat gacgcgtgaa caggcaatca
     6841 gcatttaccg taaagcgttt tgggagcgtt accgcgccga ccaaatgccg gaagcggtcg
     6901 cgttccaatt ttttgatgcc tgcgtcaacc acggttacgg caatgccgcc cgtatgctgc
     6961 aacgcgccgc aggcgtaccg gacgacggcg ttatcggagc agtcagcctc aaagccatca
     7021 attcccttcc cgaaaacgac cttttattgc ggttcaacgc cgagcgtctg gtcttttata
     7081 ccaagctcgg tacgttcacc tctttcggca agggctgggt acgccgtgtg gcgcaaaacc
     7141 tgattcacgc gtctgcagat aacactgatt aaagggagat aaaccatgtc aaaaaagtca
     7201 ctcatcgccc taatgaccgc agccatgcag cccgatttca gccacagcga cctaggcatt
     7261 cgctacgcca tgccgactca gggatgttgg acgcaagccc accgcaagag cggggtagcc
     7321 gccgcgaaac gcgcagccaa aaaaaagcgt cacaaataac cgcctttttc cgatggctgg
     7381 gcggcttggt ctctaatccg gccacaggaa aaatcagcca taccaaacta tgggcaaacg
     7441 tcgccgcagc cgccatgact tggaagttcg tgcaggcggc ggacgcgccc gaatggctct
     7501 agtgggctta tggcgcattg gtcggcgggt atgcattaat caaacgcggc atcgcggcga
     7561 ttccgcagtt ggcagaaatc aaaaaatccg caaatcagga aggggggcgg caatgattga
     7621 atttgtccga gccaaaaaac ggctgctttg ggcatttgtg cttttgcttg tgtggacgtg
     7681 cggttaccga tacgccgccg acaaggccga agcgaaacaa accgccctga ttgccaccta
     7741 tcggcattct tctatggttg cggcggaaca atatgccttg cagcttaaaa aagcgcagga
     7801 cgaaaggcag cggtggtacg acttttccca aaaacaagga agaaagcccg tgaaaaaaca
     7861 gtatccgccg caaacgaaaa aagccggcta tctgaaaacc aaggaagaac tgcttgcgga
     7921 attggcttgc cttaaagcgg aaatggttgc cctaaaaaag cccgatgcct taatccatgg
     7981 gaaagaagtg cggcagaaag aacgcaactc gtcgcagggt taaggcaatg ccatccgttg
     8041 aactgctgtt ggagattgtc cttctattac caattggccg tccaatcggc agaagacaaa
     8101 tatgccgatt tgaaacggca tatccatgat atttatcgac gacataaggg aagatacggc
     8161 taccggagga ttgcggcagc catccgtcac gcaggaacac cggtcaatca caagaaagtc
     8221 agccgtctga tggcgaagac ggggctgaag gcagtgatac ggcggcgcaa ataccgctcg
     8281 ttcaaaggag aagtcggcaa aattgcgccg aatatcctgc gacgctgttt ccatgcagaa
     8341 aagccgaatg agaaatgggt aacggacgtt accgagttca atgtaggcgg agaaaagata
     8401 tacctttctc cgattatgga tttgtttaac ggggaaatcg tcagttaccg tattcaaatc
     8461 cgcccgactt tcgatttggc cggcgagata ctgaaaggtg cgccggagaa accgggatcg
     8521 tctgaaaagc cgatactgca ttcggatcaa ggttggcaat atcagatgtt ttttatcaaa
     8581 agcagttgaa ag
//
LOCUS       DAWIVB010000079         8568 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWIVB010000079 DAWIVB010000000
VERSION     DAWIVB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.079"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WIX60_001230"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WIX60_001230"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ5305497.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="WIX60_001231"
     CDS             1160..2290
                     /locus_tag="WIX60_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ5305498.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFIEGLLGRLLDPQDPTARALLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTVEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRIAALEAQFKNALLNASPDFQDEYGYEKDAPGEANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2361..3212
                     /locus_tag="WIX60_001232"
     CDS             2361..3212
                     /locus_tag="WIX60_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ5305499.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIRLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFTVIWLFSKKQ
                     RSTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="WIX60_001233"
     CDS             complement(3295..3957)
                     /locus_tag="WIX60_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ5305500.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4052..5845)
                     /locus_tag="WIX60_001234"
     CDS             complement(4052..5845)
                     /locus_tag="WIX60_001234"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ5305501.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6090..7199
                     /gene="proB"
                     /locus_tag="WIX60_001235"
     CDS             6090..7199
                     /gene="proB"
                     /locus_tag="WIX60_001235"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ5305502.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7212..8474
                     /locus_tag="WIX60_001236"
     CDS             7212..8474
                     /locus_tag="WIX60_001236"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ5305503.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAATADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPNPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPIRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVW
                     HIETHSTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1919 a         2430 c         2248 g         1971 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccag acaaacaggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaacctgcc
      181 gtcgatgata tgcgtggctt tcacaccgtt gacggcggct tcgaccgcag aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttcg gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctt acgccgaccg cgtgtccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacatgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaactcac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtatt tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgccttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacggctccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca gactttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgatc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaat catactccgc tgtccaacag cgtgtattac gcctatttcg aaccttactc
     1501 cgaagaacag catttaaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aagggcgcga catcaatctg ctgaccatag gcaatcaggt
     1621 cgaaagcgat ttgaaaatct ggattaccgc ccgccagcat ccgggagaaa ccatggcgga
     1681 atggtttatc gaggggctgc tcggcagatt actcgacccg caagacccta ccgcccgcgc
     1741 cttgctcgac cgcgccacgt tctatatcgt ccccaatatg aaccccgacg gttcggcact
     1801 gggcaacctt cgtaccaacg ccgccggcgc gaacctcaac cgcgagtggg aaaacccgac
     1861 ggtagaaaaa agcccggaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggagtgga
     1921 tttgtttttg gacatccacg gcgacgaagg gctgcccttt gtctttgtcg caggtacgga
     1981 aggcgtgccg aactacaatc cgcgcatcgc cgcgttggaa gcgcagttta aaaatgccct
     2041 tttaaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgccgggcga
     2101 agcgaatatg accttggcga ccaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gctggaaatg ccgttcaaag acaatgccaa tctgcccgac gacgacttcg gctggaacgg
     2221 tcagcgttct ttacggctgg gcgaagccgc gttgtccgct atcctcaacg tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcccgtt atgattaccc atccccaatt cgatcccgtc cttatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgctcg
     2521 acgacttcct gacatggggc attttgggcg taattttggg cgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatacgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggatacggt cgcaccgctc gttccgctgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagat gccgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcaccgtc atttggctgt
     3001 tctctaaaaa acagcggtcg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa ttcgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gtatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggtttgttg atgactgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagcgc cgttttcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac
     3601 aatatccaaa ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccctat tatcgccgca atgccgtctg aagccttttt
     4021 atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgccc tgcgctttcg
     4081 gtttggcggc actaaattcc agcttgctca aagcggaaag gtaggctttg gcggtggcga
     4141 ccaaaacgtc ggtatccgcg ccctgaccgt tgacgacgcg gttgccgcgc gccagacgga
     4201 cgctggtttc gccctggctt tccgtacctt gcgtgacggc gttgacggaa taaatctgca
     4261 aagccgcgcc gctttgcgcc acgctttcaa tcgctttgaa aatcgcatcc acggggccgg
     4321 aaccggttgc ggaagcgcgt ttttcttcac ctttgatgct gaacacgatg tcggcgcgcg
     4381 gttcttctcc ggtttcggtg ctgatttttt gggagatgaa tttgtagctc tcggcattca
     4441 tgctgcccat ttcgtcggat accagtgcgt gcaggtcttc atcgaagatt tcgcgttttt
     4501 tgtcggcgag ttctttgaag cgtgcaaatg ccgcgttcag tgcctcttcg ctttccaact
     4561 cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat ttgcccaagc
     4621 tcaaacggtt tgttgcccag ccgaccgatt cggcggacat aatctcgtaa gtttcgcggt
     4681 gtttcagcac cccgtcctga tggatgcccg attcatgcga aaaggcattg gcaccgacaa
     4741 tggctttgtt gggctgcacg ggatagcccg taatggtgga caccagtttg gacgaaggca
     4801 cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc actttcaacg
     4861 ccatcacgat ttcttcaacc gaagcattgc ctgcacgttc gcccaagccg ttgacagtac
     4921 attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc gccaagccca
     4981 aatcgttgtg gcagtgtgcc gaccaaacga ctttgccgcc gttgggcgtt ttggcaatca
     5041 gttcgcggaa aaattcttcg gttttatacg ggatggaata gccgacggta tcgggaatat
     5101 tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcggcaagg aaatcgattt
     5161 ccgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattca cgagcgattt
     5221 tcaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcaat ttgtactcca
     5281 tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg ggcgcaacgg
     5341 cctcacccgc ctgacggatg tcccgctcga tggcgcggga caatgaacag accgttgatt
     5401 tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg gcagcggcaa
     5461 aacccgcttc gatgatgtcc acacccaatt tttccagctg gcgggcgacg cggatttttt
     5521 cctctttggt catagcggcg ccgggcgatt gttcgccgtc gcgcagggtg gtgtcgaaaa
     5581 tgataacgcg gttggtctgt gtcatttctg ttctctccag agattcgttt ttttgtaaaa
     5641 aagcattcaa atccagcggc ctgccttgcg cttccgccaa cgcggtcagc ttttgcagtt
     5701 gcgccagagg cagcgagccg cgcgtgcgcc atttatagat ggcggcggtc gtcgcggcat
     5761 tttcgggatc gtgctgtttc aacgcttcgg caagcgcgtt cacgccgccg aaataagcaa
     5821 ccaagcggtc tatatctaat tgcatggtag tccttgtcta aaataaatca gcctacagca
     5881 gaaatatgga caggattata cgcattttgg acaaaacggc aatataaatt tgaaaaatct
     5941 caataagccg ccatagattt tattaatttt atacattttg tccattttat tgcagacgcg
     6001 gcgtgattgt tgacaattgc ggcagaacag ggcaaattcg gcggcacaag gtggcaacga
     6061 gacaggacaa accaacaagg acggaaacaa tgaaatacaa aagaatcgta tttaaagtcg
     6121 gcacatcttc gattacccat tcggacggca gtctctcgcg cggcaaaatc caaaccatca
     6181 cctgccagct tgccgcattg catcatgcgg gacacgagct ggtcttggtg tcttccggcg
     6241 cggttgcggc agggttcggt gcgctgggtt tcaaaaaacg tccggtcaaa atcgccgaca
     6301 aacaggcttc cgccgccgtc gggcaggggc tgctgatgga agaatatacg gcaaacctgt
     6361 cttcagacgg catcgtgtcc gcgcaaatcc tgctcagccg cgccgacttt gccgacaaac
     6421 gccgctacca aaatgccggc ggcgcacttt ccgtgctgct gcaacgccgc gccgtcccca
     6481 tcatcaatga aaacgatacg gtttcggttg aggaattgaa aatcggcgac aacgacacat
     6541 tgagtgcgca agtggcggcg atgatacagg cagacctctt ggtgctgctg accgacatag
     6601 acggtcttta cacgggcaac ccgaacagca atcccgatgc cgtacggctg gacaaaatcg
     6661 aacacatcaa ccatgaaatc atcgaaatgg cgggcggctc gggttcggca aacggcacgg
     6721 gcggtatgct gaccaaaatc aaagcggcaa ccatcgccgc cgaatccggc gtaccggtgt
     6781 atatctgttc ctcgctcaaa cccgatgcac ttgccgaagc tgccgaacat caggcggacg
     6841 gctcgttttt cgtcccccgt gccaaaggtt tgcggacgca gaagcaatgg ctggcgttct
     6901 attccgaaag ccggggcagc gtttatgtgg acgaaggtgc ggaacacgct ttgtccgaac
     6961 aggggaaaag cctgctgatg tcgggcattg ccggaatcga agggcatttt tcccgtatgg
     7021 acaccgtaac cgtgtacagc aaggcaacca aacagcccct gggcaaaggg cgcgtcctgt
     7081 tcggctctgc cgccgccgaa gacctgctca aatcgcgtaa ggcgaaaggc gtgttcatcc
     7141 atcgggacga ctggatttcc atcacgcccg aaatacgcct gcttctgacc gaattttaga
     7201 aaaggaaacc catgtcaaac acacaaaaac agcttgccct tgccaaagcg gcaaaaaaat
     7261 ccgtcaacac ggcggatacg gaagaaaaaa accgcgcgct gcttgcgatg gcggacagct
     7321 tggaagcggc gacggcagat attttggcgg caaaccgtca agacttggaa gccgcggcag
     7381 gcaacattcc cgaaagcatg accgaccgcc ttttgttgga cggcaaacgc atttgcgcga
     7441 tggcggacgg catcagggcg gttgccgcgc tgcccaaccc cgtgggcgaa atactggaaa
     7501 cctcgacttt gcccaacggc ttggaaatcg tcaaaaagcg cgtggcgatg ggcgttatcg
     7561 gcattattta cgaaagccgc ccgaacgtta cttccgatgc ggcggctttg gcactgaaaa
     7621 gcggcagcgc ggtcgtactc cgcagcggca aagatgcatt ccaatccgca cgcgccatcg
     7681 ttgccgccct gaaaacgggg ttggcgcaaa cccgcatcga ccccgacgcg ctccaactga
     7741 ttgaagacac cgggcgcgaa agcagttacg aaatgatgag ggcgaaagat tatctagacc
     7801 tgctgattcc gcgcggcggg gcggggctga tacgggcggt ggttgaaaat gccgtcgtgc
     7861 cggtcattga aacgggaacg ggcattgtcc acatttatat cgacaaggac gcggattggg
     7921 acaaggcgct ccgtatcgtg tacaacgcca aaaccagccg tccgtccgtg tgcaactcga
     7981 tggaagtgct gctggtacat gaagacattg ctgccgactt cctgcccaag ctcgaacggc
     8041 tgttggttcg cgaccgcata gaagccggac tgccgcccat ccgcttccgt ttggatccgc
     8101 aggcggcgcg gcatatcggc ggcgaagcgg cgggtgcaga cgatttcgat accgagtttt
     8161 tagactacat cctcgctgtg aaaaccgtcg cttcggtcga agaggcggtc tggcacatcg
     8221 aaacccacag cacacaccat tcagacggca tcgttaccga aaaccgccac gctgcggact
     8281 atttcacgac tcatatcgat tctgccgccg tgtatgtcaa cgcgtccacg cgctttaccg
     8341 acggcggcga attcggcttg ggttgcgaaa tgggcatctc cacgcaaaaa ctccacgccc
     8401 ggggcccgat gggtttgaaa gagctgacga gttacaaata catcgtacaa ggcacgggac
     8461 aggtcaggga ataatccgaa ttcaaatgtc tgatatagtg gattaacttt aaaccggtac
     8521 ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctga
//
LOCUS       DAWIVB010000080         8431 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWIVB010000080 DAWIVB010000000
VERSION     DAWIVB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.080"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            31..168
                     /locus_tag="WIX60_001237"
     CDS             31..168
                     /locus_tag="WIX60_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-like fold family protein"
                     /protein_id="HEZ5305504.1"
                     /translation="MHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVF
                     G"
     gene            complement(376..1761)
                     /gene="pntB"
                     /locus_tag="WIX60_001238"
     CDS             complement(376..1761)
                     /gene="pntB"
                     /locus_tag="WIX60_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ5305505.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1841..2224)
                     /locus_tag="WIX60_001239"
     CDS             complement(1841..2224)
                     /locus_tag="WIX60_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ5305506.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2228..3769)
                     /locus_tag="WIX60_001240"
     CDS             complement(2228..3769)
                     /locus_tag="WIX60_001240"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ5305507.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAVPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            4059..4892
                     /gene="serB"
                     /locus_tag="WIX60_001241"
     CDS             4059..4892
                     /gene="serB"
                     /locus_tag="WIX60_001241"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ5305508.1"
                     /translation="MPHALVLQFPSAAALPSDFPLRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKNKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDERVLADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAVGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4956..6179
                     /locus_tag="WIX60_001242"
     CDS             4956..6179
                     /locus_tag="WIX60_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ5305509.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGVY
                     TSFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGKRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLTV
                     NTSLLWFSAVIGPILGVAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVISVWFP
                     EILGNGKAGNQLTFGGLTDWQHSLGLTAVKWLVVLMALAVGAYGGLITPSMMLGSTIA
                     FAAATAWNSVFPEMSSESAAIVGAAVFLGVSLKMPLTAIAFILELTYAPVALLMPLCT
                     GMAGAVWVAKKMGFK"
     gene            6343..7923
                     /gene="purH"
                     /locus_tag="WIX60_001243"
     CDS             6343..7923
                     /gene="purH"
                     /locus_tag="WIX60_001243"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ5305510.1"
                     /translation="MSSIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGTPEIAGFPGRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DVPACVIVKHANPCGVAIASNTLDAYKLAYATDTTSAFGGIIAFNREVDGATVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1904 a         2285 c         2346 g         1896 t
ORIGIN      
        1 tttgaaggca aaggcgttga tgttttgtat atgcacttcc acaaagccaa cgagtttttg
       61 ggtatgaccc gcctgccgac attcttatgt aacgatgtgg ttaaaaatcc gcaagtggaa
      121 aaatacttgg cagattatca ggcacacttg gaaaaagtgt tcggctaatt aaaaatccat
      181 cttcaacacg gagatggatt ttgtttgttt cgttgatttt gtgtcagttt cagatgtagg
      241 tgcttattcg gacgggccgt ctgaaaatgt ttgccccaat gcaaaaaaat cactgcaaac
      301 cttcataaac ggggtttgca gtgatttttt caaatcaaac agattgaaaa cctgcgccga
      361 attgttcaga cggcattatt ttttcagttc ggacagaatg tcatctacgg ttttcttcgc
      421 atctccgaaa cacatcacgc tgttttcgtt gaagaacagt gggttttgta cacctgcgta
      481 gccggtattc atcgagcgtt tgaagacgac gacttctttt gccttccaca cttccaacac
      541 gggcataccc gcaatcgggc tgttcgggtc ggtttgggcg gcggggttga cggtgtcgtt
      601 cgcaccgatg accaagacca catcggtttc ggggaagtcg tcgttgattt cgtccatttc
      661 caaaacgatg tcgtagggga ctttggcttc ggcgagcagt acgttcatat gaccgggcag
      721 gcggccggcg acggggtgga tgccgaagcg tacttcggtg ccgtttttac gtaaaagctc
      781 ggtgatttcg gcaacggggt attgcgcttg tgcgactgcc ataccgtagc ccggggtaat
      841 gatgacattg tttgcgcctt tcagcatttc ggcaatatcg gcagctttga cttctcggta
      901 ttcccctatc tcttggctgc cggaagataa tgtgccgctg tcgctgccga aaccaccggc
      961 aattaccgag acaaacgagc ggttcatggc tttgcacata atgtaggaca gaatcgcgcc
     1021 gcttgagccg accagcgcgc cggtaacgat gagcaggtcg ttggagagca tgaagcctgc
     1081 cgctgcggcc gcccagccgg agtaggagtt gagcatggac acgaccacgg gcatatctgc
     1141 gccgccgatg gaggcaacca agtgccagcc gaatgcgagg gcaatcaggg tcataatcag
     1201 caggatgaag ccgctgccgt caatgccgac aaatacgagc agcaacacaa acgatacggc
     1261 aagtgccagt gcgttgagct tgtgtttggc gggcagttgc agcgggctgc tgctgatttt
     1321 gccgttgagt ttgccgaatg cgaccagcga gccggtaaag gttaccgcgc cgatgaagat
     1381 gcctaaatac acttcgacca gatggatggt gtgcatatcg tgcgaaacgt tgcccggcgc
     1441 gatatagctg ttgaagccga ccaaaaccgc cgctaggccg acgaagctgt gcagcagggc
     1501 aatcagttcg ggcatttcgg tcatttccac ctttttggct ttgtagatgc cgattgccgc
     1561 gccgatgagc atggcgatga tgatccagcc cagtccgtgg gtattgtcgg aaaaaacagt
     1621 tacaaaaagg gcgaccgcca taccggcgat accggaatag cagccctgtt tggcggtttc
     1681 ctgtttggac agccccgcca gtgagaagat gaataaaatt gcggcaacga tatacgccgc
     1741 tgttacgagt cctgaagaca tagaaattct ccgattttcg atgatttgtt ttcaatgccg
     1801 tctgaaaaat tgacgttcgt gttttcagac ggcatctgtt tcaagcagcc gcgacaaaca
     1861 gcccgacggt gcaggcaaat ccgcctgccc acatcagtga gcgcatagcg gctttgtcgg
     1921 cgatatagca ccagataaag gcgaggcgga acaggatgaa caggcaggca agcgtgttga
     1981 tggtcgattg cgccgcattg ccggttgcgt gtgccgtcaa aacggcggcg gcaaacggtg
     2041 caaaggcttc aaaaccgttt tgctgtgcgg cgtgggcacg ggcggctgcg ccttgcgtgt
     2101 gcgccagaaa accgcgcgga ttgtggttgt ctttaaaccg gaatccgccc gcttttttgg
     2161 catacgccgc acaaaaaagc ggcaataggc aggcaatcag aatacaccaa taggcgaaag
     2221 tcatggctta ccctttctta aacatattca gcatacgccg tgttaccgca aagccgccga
     2281 agatgttgat gccggcaatc aggatggcaa caaacgacag cagcgaaacg aagccgttgc
     2341 cctgaccgat ttgcagcagc gcgccgacga cgatgatgcc ggagatggcg ttggttaccg
     2401 acatcagcgg tgtgtgcagc gagtggctga cgttccagac gacgtagtag ccgatgacgc
     2461 aggcgagaac gaacacgata aagtggttca ggaatgctgc gggtgcgacc gcgccgaccc
     2521 acagtaccaa gacggcggcg atgacggcgg gcgcgagttt tttccacagg ggaacgggtt
     2581 ttggctcggg cttggcggca ggcacggctt tttcagacgg cgtttgctgc ggctgggcgg
     2641 aaacttgaat cggcggaggc gggaaggtga tttcgccgtc gtgggtaacg gtcatgttgc
     2701 ggataatcac gtcttcgaag tccaacgtga tttcgccgtc tttgttcggg cttaacagct
     2761 tggtcaggtt gaccaagttg gtggcgtaaa gctgggaaga ctgtccggca aggcggtttg
     2821 ccatgtcggt gtagccgatg attttcacgc cgttgccggt tacggacaat tcgcccgggc
     2881 gggtgagttc gcagttgccg cccgtcgccg ccgccaaatc gacgatgacg gagccggatt
     2941 tcatgctttc caccatttct ttggtaatca gcttgggcgc gggtttgccc ggaatggcgg
     3001 cggtggtgat gatgatgtcc acttctttcg cctgctcggc aaagagcttc atctcggctg
     3061 cgataaattc gtcgctcatc actttggcgt agccgtctcc gctgccgccc gattcttgtg
     3121 ggaagtcgag tttcaggaac ttgccgccca tcgattcgat ttgttccgcc acttccaagc
     3181 gggtatcgaa cgcgcgtacc actgcgccga gcgagtttgc cgtaccgatc gccgccaaac
     3241 ctgccacacc tgcaccaatc accaaaacct gcgcgggcgg cactttgccg gcggcggtaa
     3301 tttgaccggt gaagaaacgg ccgaaggcgt tggcggcttc aattacggcg cggtagccgc
     3361 tgatgtttgc catcgaagac aaagcgtcca aagcctgcgc gcgcgaaatg cggggcacca
     3421 tatccatcgc cagcgcgttc actttcttgg cgcgcaaggc ttcgaccaaa gcctcgtttt
     3481 ggcgcggcca caggaagctg acgatggttt gaccttcgtt caaaagcggc agttcctgtt
     3541 cggacggcgc gttgacctta taaatcaaag ggcagaccca aaccgccgct ttgtcggcaa
     3601 cggttgcgcc tgctgtttgg taagcggcat cgtccaaact tgccgccaaa cctgcaccgc
     3661 tttcgacaac ggtttcaaag cccagtttgc ccagcagggc gacggtggcg ggcgtacagg
     3721 cgacgcgggt ttcgccggat aatgactcgc gtgggatacc gattttcatc tctgaatcct
     3781 ttttcgggtt gtttatatgt atcgtgggtt aaatttaaat cggggcgggg cggagcaacg
     3841 ccgtaccggt ttaaagccga ctcacttcaa atgttaatat attttagata atccccttat
     3901 aacgaatttt catcaggctg gcaatagttg cggcattttc ccgtgttgtc cgacacatac
     3961 tgccgtttgt gtttgaaatc gttttccgat tgcagtcccg cccgccgtaa cattctttgc
     4021 cgtttcacta tataatccgc attttttgag ccgccgttat gccgcacgcc ctcgtcctcc
     4081 aatttccctc cgccgcagcc ctgccttccg acttcccctt acgcctgccc gaacctgatt
     4141 gcgccgatga aaagcgtatg cgttttatcg ttgaagaagg gttttcttta agcgaaaaag
     4201 acgcggcgtt gcttggcagc cgtcaaatcg accacgccgt gttgccggat atggatttcg
     4261 acgaactcgg tttgattgtc agcgatatgg attcgacgct gattaccatc gaatgcgtcg
     4321 atgaaattgc ggcaggcgtg ggtttaaaaa acaaagtagc ggaaattacc gagcgttcga
     4381 tgcgcggcga actcgatttc gaacagtctt tacgcagccg cgtcgcgctg ttggcgggat
     4441 tggacgaacg ggttttggcg gacgtttatg aaaacgtttt gaagctctcg cccggtgcgg
     4501 aatttttgtt ggacgaatgc aaaaggcacg atgtgaaatt cctgctggtg tcgggcggct
     4561 tcacgttttt taccgaaagg ctgcaacaac gcctcggctt cgaataccaa cacgccaatg
     4621 ttttggaaat tgaaaacggc aggctgaccg gccgtctgaa aggcagaatc atcgacgcgc
     4681 aggcaaaggc agatttgttg cgcgaatacc gcagccgcct cggattgcag ccgcatcagg
     4741 tgttggcggt gggcgacggt gcgaacgata ttccgatgct caaagaagcg ggcataggcg
     4801 tggcttaccg tgccaaaccg aaagcgcggg ccgccgccga tgcctgtatc aacttcggcg
     4861 gtttggagcg tgtacgcggc ctgttcggat aggcggatag gaaacggatg tcgtccgaaa
     4921 ggttttcaga cggcatttga acggcaggaa cgacagtggg acgcagaaaa ctttggtttg
     4981 ccctggcagc agcgggcgtt atcggcggtt tggtcggcat tgtgctgacg gaactgatgc
     5041 acttcataca gcatacggca tacggttatg gcgcggacgg cgtgtacact tcgttccgcg
     5101 aaggcgtggc acaggcttcc ggtatgcggc gcgttgccgt gctgacgctg tgcggcgcgg
     5161 tcgcaggcag cggctggtgg ttgctgaaac gtttcggcaa gccgcaaatc gaaatcaaag
     5221 ccgccttgaa acagccgttg caggggctgc cgtttctgac gacggttttc catgttctgc
     5281 tgcaaatcat aacggtcgga ctcggttcgc cgctcggacg cgaagtcgcc ccgcgcgaaa
     5341 tgaccgccgc gtttgctttt gccggcggca aacgcttggg tttggatgaa ggcgaaatgc
     5401 ggctactgat tgcttgcgct tcgggtgcgg gtttggcggc cgtgtataac gtgccgctcg
     5461 cctccacact tttcattctc gaagccatgc tgggcgtgtg gacgcagcaa gccgtcgccg
     5521 ctgcattgtt aacttcagtc atcgccaccg ccgtcgcgcg catcggcttg ggcgacgtgc
     5581 agcaatatca tccggccaac cttaccgtca atacttcatt actttggttt tccgccgtca
     5641 tcggcccgat actgggcgta gccgccgtct ttttccagcg taccgcccaa aagttcccct
     5701 ttatcaagcg cgacaatatc aaaattattc ccttggccgt ctgtatgttt gcactcatcg
     5761 gcgtgatttc cgtttggttt cccgaaattt tgggcaatgg caaagcaggc aatcaactga
     5821 cctttggcgg attgaccgat tggcaacaca gccttgggct gaccgccgtc aaatggctgg
     5881 tcgtcttaat ggcgcttgcc gtcggcgcat acggcggtct gattaccccg tccatgatgc
     5941 tcggcagtac catcgccttt gctgctgcca ccgcgtggaa cagtgttttt cctgaaatgt
     6001 cctctgaaag cgcagccatt gtcggcgccg cagttttcct cggtgtttcc cttaaaatgc
     6061 ccctgaccgc catagccttt attttggagc tcacctacgc ccctgttgcc ttgctcatgc
     6121 cattatgtac aggcatggca ggtgcagtat gggtggcaaa gaaaatggga tttaaatagg
     6181 caaaagcaaa aggccgtctg aaaccaagtt tcagacggcc ttttacaata aaattgttaa
     6241 caatatttgc aaaaacctac tgccaaaaat gcgaaactgg gggataatac cgccctgaaa
     6301 attcatccca tactgattaa accttcaaca aaggaaatcc aaatgtcttc catcaaacgc
     6361 gccctgatca gcctatccga caagacaggc gcagtcgaat ttgcccaaac cctgcacaaa
     6421 ctcggtgtcg aaattctttc taccggcggt acagcaaaac tcttggctga tgcaggcgtt
     6481 cccgttatcg aagttgccga ctataccggt tttcccgaaa tgctcgacgg ccgcgtgaaa
     6541 accctgcatc cgaaaatcca cggcggtatt ctcggtcgtc gcgatttgga cgaacacgtc
     6601 gccaagatgg aagaacacgg catcggcaat atcgacctcg tgtgcgtcaa cctctacccc
     6661 ttcgctgcca ccatcgccaa accaaactgc acgctggaag acgcgattga aaacatcgac
     6721 atcggcggcc cgaccatggt gcgctctgcc gcgaaaaact ggaaacacgt cgccatcgtt
     6781 accgacaccg ccgatttccc ggccatagct gccgaactcg aagccaacaa cggcgcattg
     6841 agcgacaaaa cccgtttcaa cctctcgcgc aaagcattca gccataccgc ccaatacgac
     6901 ggtatgattt ccaattacct gacctcgctt tcagacgacg tcttgagcgg cacgcccgaa
     6961 atcgccggat tccccggccg gttcaatcaa agctggatta aagtgcaaga catgcgctac
     7021 ggcgaaaacc cgcatcagcg cgccgcgttc taccgcgata ttgaccccgc cgcaggcagc
     7081 ctttccgcct acaaacaact gcaaggcaaa gaattgtctt acaacaacat cgccgatgcc
     7141 gatgccgcat gggaagccgt caaatccttc gacgtgcccg cctgcgtgat tgtgaaacac
     7201 gccaatccgt gcggcgtagc catcgcctcc aataccttgg atgcctacaa actcgcctac
     7261 gccaccgaca ccaccagcgc gttcggcggc atcatcgctt tcaaccgcga agttgacggc
     7321 gcaaccgtca aacaaattac cgacaaccag tttatggaag tcctcatggc gcctaagttc
     7381 accgccgaag ccctcgaaat cgccgccgcc aagaaaaacg tgcgcgtatt ggaagtgccg
     7441 cttgaggcag gcgcaaaccg cttcgaactc aaacgcgtcg gcggcggact gttggtgcaa
     7501 acgcccgaca tccaccgcat cagccgcgcc gatttgaaag tcgtctccaa acgccaaccg
     7561 accgagcagg aatggaacga tttgctgttc gtctggaacg tcgccaaata cgtcaaatcc
     7621 aacgccatcg tattcggcaa aggcggtcaa acctacggca tcggcgcagg ccaaatgagc
     7681 cgcgtggaca gcacccgcat cgccgcccgc aaagcgcaag atgccggtct cgacctcaac
     7741 ggcgcgtgtg ccgcatccga tgccttcttc cccttccgcg acggcgtgga cgtgattgcc
     7801 gaacagggca tcaaagccat catccatccg gcaggctcga tgcgcgatca ggaagttttc
     7861 gacgcagccg acgaacacgg catcgccatg gtcgtaaccg gcatccgcca tttccgccat
     7921 tgatgcagat aaacaaggta atgccgtctg aagggctttc agacggtatt ttgcgctatt
     7981 ttgcgaaggt agggatgacg gttcgggtat tcctgacagg gtggattttc aaggtgttgt
     8041 atagggtgta ggaggattcg taaaaggtgg gatgcagggt gtgcttcagc ccgctgcatc
     8101 aaaaattttt ggagaaccgg cgggagtcgg cggttttggt ttcggcgggg acggtggaaa
     8161 tgggtaacat tgacggaatc gacggaagcg gtggactgaa gcccaccctt gtatattgga
     8221 atcaccgtat catagcaaca aaccgcccag ccgccacccg cgcccaccca aggcacacaa
     8281 ccgttgcgta gctcagggag cggcagggca acccatcgac acaaccggac agttgccgga
     8341 caacacaacc gaatgcaagg caggtttatg atgagtaccc aataccatta cgcaggtata
     8401 gtgaattaaa tctaaaccag tacagcgttg g
//
LOCUS       DAWIVB010000081         8364 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWIVB010000081 DAWIVB010000000
VERSION     DAWIVB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.081"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(457..732)
                     /locus_tag="WIX60_001244"
     CDS             complement(457..732)
                     /locus_tag="WIX60_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ5305511.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(729..2012)
                     /locus_tag="WIX60_001245"
     CDS             complement(729..2012)
                     /locus_tag="WIX60_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEZ5305512.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAVYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWLEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2168..3586)
                     /gene="glnA"
                     /locus_tag="WIX60_001246"
     CDS             complement(2168..3586)
                     /gene="glnA"
                     /locus_tag="WIX60_001246"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ5305513.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGETAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3670..3855
                     /locus_tag="WIX60_001247"
     CDS             3670..3855
                     /locus_tag="WIX60_001247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305514.1"
                     /translation="MPSETGFASNFKPENIVSDGKIVNNADKPAHRIRIFPDRYRKRC
                     RLKAVSDGIVSICTPPL"
     gene            3886..4695
                     /gene="aroE"
                     /locus_tag="WIX60_001248"
     CDS             3886..4695
                     /gene="aroE"
                     /locus_tag="WIX60_001248"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ5305515.1"
                     /translation="MTALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAL"
     gene            4698..5399
                     /gene="mtgA"
                     /locus_tag="WIX60_001249"
     CDS             4698..5399
                     /gene="mtgA"
                     /locus_tag="WIX60_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ5305516.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LKRMGSAELPESDTD"
     gene            complement(5602..5677)
                     /locus_tag="WIX60_001250"
     tRNA            complement(5602..5677)
                     /locus_tag="WIX60_001250"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5642..5644),aa:Phe,seq:gaa)
     gene            complement(5868..6602)
                     /gene="lptB"
                     /locus_tag="WIX60_001251"
     CDS             complement(5868..6602)
                     /gene="lptB"
                     /locus_tag="WIX60_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ5305517.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(6676..7206)
                     /gene="lptA"
                     /locus_tag="WIX60_001252"
     CDS             complement(6676..7206)
                     /gene="lptA"
                     /locus_tag="WIX60_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ5305518.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7187..7768)
                     /gene="lptC"
                     /locus_tag="WIX60_001253"
     CDS             complement(7187..7768)
                     /gene="lptC"
                     /locus_tag="WIX60_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ5305519.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7765..8301)
                     /locus_tag="WIX60_001254"
     CDS             complement(7765..8301)
                     /locus_tag="WIX60_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ5305520.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPGAHWFTRQHAAYITEHAGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         1952 a         2276 c         2144 g         1992 t
ORIGIN      
        1 cttacaatat aaaacaaaca aatatagtgg attaacaaaa accagtacgg cgttgtctcg
       61 ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
      121 cactataagt tgacatattg tgcgatgtcg agggctgtac cgtattttcc catcgtattt
      181 tcccgttctt tatatgagcg gcaggcatcc tcataaaaga agggataccg ataaggaaaa
      241 ataccccggc ataaaaaata tactgattaa tcaatacaaa tccaacaaac agttcatcgt
      301 attcttttct gttggcgtat aaaaaatact gatatatgcc ttcactttca tagggcgacg
      361 ctttatgtac cgtcctgtgt gttgaaacgt cggttttcct taccgcctgc cccgcttttc
      421 cctgattgct gccaactgcc gtccgaacct gaaccgtcag gtttcagacg gcattttttc
      481 tgcctgctcc cacaattgcc gtttttgtgc ggcggtcagt tttttgacgg cgatttcctg
      541 cctgagtgcc gttcctttat ccatcccgcc tgcaacgata cgcatcgcca ccggtttgaa
      601 tacgcgggta tatttcgcgc ctttaccggt tgtgtgggcg gcaagccgct gttgcggatt
      661 cgggctgatg ccgcaataga acgcgctgtt ttcacacaat atcaggtaaa cgctccagtt
      721 tgacgcgttc atctgcctgc atcctgagtt ttctccccgt tccaaggcgc aactttcagc
      781 agcagcagca tacccggtag ggcgagtgcg aaacacagtt ggaaaaacgg tacataaccg
      841 agccattcaa tcagataacc ggcaaaggaa ttgatgaccg tgcgcgggac ggcggacagg
      901 ctggtaaaca gcgcaagctg cgttgcggta aatgcgggat tggtttcacg cgccatatac
      961 gatacgaacg ccgccgtccc caaccccacg ccgaccgctt ccgcgccgat aactgccgcc
     1021 agcatcagcc tctcgcctgt gccgaccgtg tcgaaaggtc cgaaccctgc cagccataca
     1081 aaccccaaaa cggttacagc ctgcaccgcg ccgaatagcc acaaggcttt gtttacgccg
     1141 attttcagca tccacacacc gcccaagata cctgccgcca ctgccggcca cagtcctgca
     1201 tttttcgcaa tcaaaccgat gtcggtcttg ctgaaaccca tatccagata aaacggcgtt
     1261 gccaacgcgg ttgccatact gtcgccgagt ttgtaaagga agataaacag cagcacgcac
     1321 accgccgaag cgatgccctt gcgcgtaaaa aattctttaa acggctctac cacggtctgc
     1381 ttcaacgttt taggaacggc aggaggcaac acgggttcgc gcgcaagaaa cagcgtcatc
     1441 agaaggccgg gcagcataaa taatgaagtg ataacaaata cttctaacca cggcatcctg
     1501 tctgccaaca ccaaactcaa tgaaccggga atcagggcgg caatccggta ggcgttcaca
     1561 tgaaccgagt tgcccaaacc caattcttcg tctgacaaaa tctcgcgcct gaacgcatcc
     1621 aatacaatat cctgactggc ggaaaaaaaa gcgacaagca ccgacaagcc ggcaatcagc
     1681 ggcagatgat tacgggggtt taaaaaggca tagaccgcca aagccgccag caaccctgcc
     1741 tgcgtcagca gcatccaccc gcgccgccgt cccaaaacgg gcagcctgac cgcgtccatc
     1801 agcggcgacc acaaaaattt ccaagtaaac ggcagaccga ttaacgccat cagcccgatg
     1861 ctcttcaaat ccacctgctc gctgcgcaac cacgccggaa tcaggttaat cagaaagtac
     1921 agcggcagcc ccgaggtaaa ccccgtaaaa atacagacaa gcatattgcg ggaaaagatt
     1981 tgcccgataa aacctgattt cgatgcagtc atcgttcgtg cagcccaaaa aaaacaaaag
     2041 cctattgtag caaatatcgc ccgcctgctg aaaaatgccg ccccgcgccg ccggtttgcc
     2101 gttcaaatgc aaaatgccgt ctgaaactgt gttcagacgg cattgctttt ctgaaaacca
     2161 gacgtgctta caggctgtaa tacatttcaa attccagcgg gtgcggcgcc atgcggatgc
     2221 ggcgtacgtc ttcttctttg aaagcgatgt agctgtcgat ccagtctttg ctgaacacgc
     2281 cgccgcgcag gaggaattcg tggtcggctt tgagggcggc cagtgcttct tccaaagaag
     2341 cgcaaacggt cggcaccaat gcatcttctt ccggcggcag atcgtacagg tttttatcgg
     2401 cagggtcgcc cggatggatt ttgttttgaa tgccgtccaa acccgccatc aacagggcgg
     2461 caaatgccaa atacgggttg gcggtcggat cggggaaacg cgcttcgatg cggcgcgcct
     2521 tgctgctgtt cacggacgga atgcggatgg aagcggaacg gtttttggcg gagtatgcca
     2581 gtttggtcgg cgcttcaaag tgcggcacga ggcgtttgta ggagttggtg gacggattgg
     2641 taatcgcgtt caaggctttg gcgtgtttga tgatgccgcc gatgtagtag agcgcggtgt
     2701 cgctcaagcc ggcatagccg tcgcctgcga acaggttttg accgtctttc caaatggatt
     2761 ggtgaacgtg cataccgctg ccgttgtcgc ccataatggg tttgggcatg aaagtggcgg
     2821 ttttgccgaa gttgtgggca acgttttgaa tcacatattt catgtcttgg gtttggtcgg
     2881 cgcgtttgac caaagtagca aagcgcgtgc cgatttccat ttggctgccg gtgccgactt
     2941 cgctgtggtg cacttccact tcaataccga gttcttccaa aatgtttacc atcgccgaac
     3001 gcaaatcctg accgcagtca atcggtgcaa caggtgcgta accgcctttg acggtcgggc
     3061 ggtggccggt gttttgaccg tccatatgca gaccgcttgc ccacgcgccg ctttcggacg
     3121 tgatttcgta acgggttttg tgcatatcgg tttcaaattc tatgccgtcg aatacgaaaa
     3181 actcgggttc gggaccgaaa taggcggtct cgccgatgcc ggaagatttc aaataggctt
     3241 cggctcggcg ggcgatggag cgcgggtcgc ggtcgtaacc ctgaccgtcg gcgggatcga
     3301 taacgtcgca agtcaacaca acagtcgcat catcataaaa aggatcgacg aaggctgtag
     3361 acgcatcggg gcgcaactgc atatcggaag cctgaatgcc tttccagccg ccgatagacg
     3421 aaccgtcaaa cgcctgaccg ttttcgaacc actcttcggg gtcttccaac acgatgcgcg
     3481 caggcacggt aaagtggtgc tgcttgcctt tggtatcggt aaagcgcaaa tcgacaaagc
     3541 gggcttcgct ttcttcaatc aattttacgg cgtttttaat agacattttt cagctcctgg
     3601 aaaagggaaa tagcaaaata tgcggaacgc ggcggcttga cgcaaagtcg gcacgatttt
     3661 accacgccga tgccgtctga aaccggtttt gcgtcaaatt tcaagccgga aaatatagtt
     3721 tcagacggca agattgtcaa caatgcggac aagccggcgc accgcatccg gatttttccc
     3781 gaccgataca ggaaacgatg ccgtctgaag gcggtttcag acggcatcgt ttccatctgt
     3841 acgccgccgt tataaaatag ccttcccgtc ttttggaaca cgaccatgac cgccctcccc
     3901 cgctacgccg ttttcggcaa ccccgtcgcc cacagcaaat cgccgcaaat tcatcaacaa
     3961 tttgcccttc aggaaggcgt tgacattgaa tacgaacgca tttgcgccga catcggcggt
     4021 ttcgcgcagg cggtttcgac attttttgaa acaggcggtt gcggggcaaa cgttaccgta
     4081 ccgttcaaac aggaagcgtt tcatctggcg gacgagcatt ccgaacgcgc attggcggca
     4141 ggtgcggtca atacgctgat tccgttgaaa aacggcaagc tgcgtggcga caacaccgac
     4201 ggtatcggtt tgaccaacga catcacgcag gtcaaaaata ttgccatcga gggcaaaacc
     4261 attttgcttt tgggcgcagg cggcgcggtg cgcggcgtga ttcctgtttt gaaagaacac
     4321 cgtcctgccc gtatcgtcat tgccaaccgt acccgcgcca aagccgagga attggcgcag
     4381 cttttcggca ttgaagccgt cccgatggcg gacgtgaacg gcggttttga tatcatcatc
     4441 aacggcacgt cgggcggtct aaacggtcag attcccgata ttccgcccga tatttttcaa
     4501 aactgcgcgc ttgcctacga tatggtgtac ggctgcgcgg caaaaccgtt tttagatttt
     4561 gcacgacaat cgggtgcgaa aaaaactgcc gacggactgg gtatgctagt cggtcaagcg
     4621 gcggcttcct acgccctctg gcgcggattt acgcccgata tccgccccgt tatcgaatac
     4681 atgaaagccc tataaacatg ttccgcatca tcaaatggct gattgccctg cccgtcggca
     4741 tctttatctt tttcaatgcc tatgtgtacg gcaacatcat tacctaccgc gccgtcgcgc
     4801 cccatcggac tgcctttatg tcgatgcgga tgaagcagtt tgaacaggaa ggtcgcgatg
     4861 tcgcactgga ttaccgctgg atgccctaca aacgcatttc caccaacctg aaaaaagccc
     4921 tgattgcttc cgaagatgcc cgtttcgccg ggcacggcgg cttcgattgg ggcggcattc
     4981 aaaacgccat caggcgcaac cggaacagcg gcaaagtgaa ggcgggcggc tcgaccatca
     5041 gccagcagct tgccaaaaac ctgtttttaa acgaaagccg cagctatatc cgcaaaggcg
     5101 aagaagcggc gattaccgcg atgatggaag ccgttaccga caaagacagg atttttgaac
     5161 tgtatttaaa ctcaatcgaa tggcactacg gcgttttcgg cgcggaagcc gcgtcccggt
     5221 atttttatca aatacccgcc gccaagctga ccaaacagca ggcggcaaaa ctgacggcgc
     5281 gcgtccccgc cccgctctac tacgccgacc atccgaaaag caaacggctc cgcaacaaaa
     5341 ccaatatcgt gctcaaacgc atgggttcgg cagagttgcc tgaaagcgac acggactgag
     5401 tgccgccgca atataaaatg ccgtctgaaa tatcattcag acggcatatg ctttctgcct
     5461 ttaccctctt ctccaaaacg caacggggcg gtcattatca tttttgccct tctacataac
     5521 ggtcagacac aaaacgtgtc cgacacggac aacttcccaa aaacggcagg caaaaaaaat
     5581 aaccgcttca aggcggtttt ttggtggcca gaggcggaat cgaaccgccg acacacggat
     5641 tttcaatccg ttgctctacc aactgagcta tctggccttt gtctgtctcg ttaagagatg
     5701 cgtattaaac caaattacgg gacgacatgc aagcatatcg ggcaatattc tttgaaaaca
     5761 ggtttaagtc aatgttttaa atttgaatta atttatgtaa aaacaacata aatccgtatt
     5821 tttgccgcct gcccgacgat attaggtcgc ccgtctgaaa aatattttca atatttgaag
     5881 ttcttaccca gataaacaga acgaacctgt tcgtttccga ccaaatcatc aggttttccc
     5941 gatgccaaca ccgtgccgtc tgaaataatg taggcccgat cgcagatgct gagggtttcg
     6001 cgtacgttgt ggtcggtaat cagtacgccg ataccgcgcg atttgaggaa accgatgatt
     6061 ttctggatgt cgatgacggc aatcggatcg acgccggcaa aaggttcgtc caacaaaata
     6121 aaatgcggtt tcatggcgag tacgcgggcg atttcgacgc gccgccgttc gccgccggac
     6181 agcgacggcg cggggctgcg gcgtaagtgt ccgatattga ggtcggcgag cagtttttcg
     6241 atttccctgt cgatttgatt tttatctttg gttctgattt ccaagatggc gcggatgttt
     6301 tgttcgacgg tcattttgcg gaatatcgag gcttcctgcg gcaggtagcc gacaccgagg
     6361 cgggcgcgtt cgtgtatggg caggtggcgc aattcttgtc cgtctagggt tacgctgcct
     6421 gcgtcggcgg cgatgagtcc gacaatcatg tagaagctgg tggttttacc cgcaccgttg
     6481 ggcccgagca gtccgatgac ttcgccgctt tcgatttcga gggagaagct tttaacgact
     6541 tggcgttttt tgaaactttt ttgcaggttt tgaacaacaa ggcggctgac gtttgcactc
     6601 ataaatatct cttcaatatc ttcggtcggc atatgccgtc cgaaccgggt ttatatttca
     6661 gacggcattt tgggattatt cggatttttg cgtactcgaa ggctggataa cgacgctgac
     6721 cctgccggat ttggaagcgg attttgcgcc ggattttgtg ctgccgctga tggtatagac
     6781 ttcggttttg gtgttgtatg taatcaccgc accttcggcg acatcgccgc cgcgctgtac
     6841 tttggcatta ccggttaaga ctacggtgct gcctgcagat gaataagcaa cgttgtttgc
     6901 ctgtccgcgc accgtgcctt tgccgccgtc caatgtctgg ctgaagcgga ctggcgaacc
     6961 ttccgccctc acggattcgc cgcctttgcc gccgcgtgta acattgacgc gggcggcgga
     7021 aatattgagc gtaccctgtc tgatgacgac gtttccgctg aatgtggtgc tttggttggc
     7081 ttgatcgagc gaaccttggt cggcctcaat ctgaataggc tgcctgctgt cgctttgaag
     7141 ggcaaaagcg ggggacgccg aaaaaaatgc tattaaaaca aatagcttac atatcttttg
     7201 tatcataaat cgtggctttc actttagatg agaagttcaa catgcctgtt ttgtggtcgt
     7261 aagtcatgcc gcccgcctga ccgtgcgatg caccatattg gaaactgaca ggcgtatcgg
     7321 tttgggcata ttgagattcg gtatcgacgt gcagcttttc ggcttcaact ttacccgcct
     7381 gccgtttgcc gtcggcggtt ttggtcagca caacgttgtt tttaaaaaga acctgtttgt
     7441 tttcggtatg gtaaacggct tcgtcgctgc cgacttcgta caacaacctg ccttcttgga
     7501 agaacacgag atgcggcgaa tcaaaatgga tgtcgctgct ttccggaaac tgtttcgcgc
     7561 ccttcgcgct caaatgttct ttcaagtatc cctgttcgtc aaaccgcctg ccgtccaagc
     7621 cgtccattgt gtattgcggt tcgtcgggat tgagcctgac ttcttcaatc tcgacttcgc
     7681 tgatacgacc caaccatgcc gacaggctgc ccaaggcaac cgccaatatc aatgggaacg
     7741 caattccgta ccgccatctt actttcattt gatgtactcg ttcaaagccg cgcccaaagt
     7801 cccttgcgcc tgcataatca ggtcgcacac ttcgcgcacc gcgcctgcgc cgcccgcgtg
     7861 ttccgtgata taggcggcgt gttgccgcgt aaaccaatgc gcgccgggga cggcaaccgg
     7921 caatccgcag cgcaccatta ccggcaaatc gaccacgtcg tcgccgacaa aggcgcactc
     7981 ggcttcttcc acgcccgcct gcgcgcgcaa ttcttcatag gcggcacgtt tgtcgctgat
     8041 acctttgaaa tagtaattta tgcccaactg tttgacgcgg atgccgacgg agggcgcgtc
     8101 ccggcccgtg ataatcgcag tctgcacgcc gcttgcctga agcattttca gaccgtgtcc
     8161 gtccagtgtg tgaaacgatt tgatttcttc gccgttatcg cggataaaga tgcgcccgtc
     8221 ggtcaaaacg ccgtccacat ccaggatcaa cagtttgatt ttggcggcgc gcgcctgtaa
     8281 ttcgggagaa attgcctgca tgggatttcc ttgttttcag acggcatatc ttaccacaaa
     8341 tgccgtctga aacggctgca acgc
//
LOCUS       DAWIVB010000082         8359 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWIVB010000082 DAWIVB010000000
VERSION     DAWIVB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.082"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..71
                     /locus_tag="WIX60_001255"
     CDS             <1..71
                     /locus_tag="WIX60_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ5305521.1"
                     /translation="QGEPNLSPKDLAGKVLSEAVTF"
     gene            96..2210
                     /locus_tag="WIX60_001256"
     CDS             96..2210
                     /locus_tag="WIX60_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ5305522.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HICKDGKEAEAVVGWGLRPTTHKARAFAAEHQLPFIALEDGFLRSLGLGVAGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQAMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGCFAKKLGKIKQLYRSFK"
     gene            2347..3606
                     /locus_tag="WIX60_001257"
     CDS             2347..3606
                     /locus_tag="WIX60_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ5305523.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            3973..5379
                     /locus_tag="WIX60_001258"
     CDS             3973..5379
                     /locus_tag="WIX60_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ5305524.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5597..6811
                     /gene="gltS"
                     /locus_tag="WIX60_001259"
     CDS             5597..6811
                     /gene="gltS"
                     /locus_tag="WIX60_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ5305525.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6910..7926
                     /locus_tag="WIX60_001260"
     CDS             6910..7926
                     /locus_tag="WIX60_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ5305526.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7937..8134
                     /locus_tag="WIX60_001261"
     CDS             7937..8134
                     /locus_tag="WIX60_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305527.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            8152..8301
                     /locus_tag="WIX60_001262"
     CDS             8152..8301
                     /locus_tag="WIX60_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305528.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
BASE COUNT         2147 a         2430 c         1935 g         1847 t
ORIGIN      
        1 tgcaaggcga gccgaacctg tcgcctaaag acttggcagg caaagtattg tctgaagcgg
       61 taacgtttta aaaataattc aggccgtctg aaagaatgtt cctcttttca gacggcctac
      121 aatccattaa taacaataat cgacgaaaac gcattgtgaa aaacgcctac atcccctctc
      181 gcggcatccg caaaatcccc catctctcca ccctattgcc tgaatttcat atctgcaaag
      241 acgggaaaga agcagaggct gttgtcggct ggggtttgcg cccgacgaca cacaaagcgc
      301 gtgcttttgc cgctgaacac cagcttccct ttattgcttt ggaagacggc tttttacgat
      361 cgctcggact gggtgtcgcc ggttatccgc cctactctat cgtctatgac gacatcggca
      421 tctactacga caccacacgt ccttcgcgtt tggaacaact gattcttgcc gccgatacca
      481 tgccgtctga aaccttggct caggcgcagc aggcgatgga tttcatcctg caacaccacc
      541 tgtccaaata caaccacgcg cccgaacttt cagacgacca tcctttacgt tccccatcca
      601 aacccgaaac cgtcctcatc atcgaccaaa ccttcggcga tatggccatc caatatggcg
      661 gcgcagacgc ctctacgttt gaactgatgt ttcagacggc cttaaatgaa aacccgcaag
      721 ccgatatctg ggtaaaaacc catcccgatg ttttgtgcgg caaaaaacaa ggctatctga
      781 cccaactggc gcagcaacac cgcgtccatc ttttggcaga agacatcaat ccgatttctt
      841 tgttgcaaaa cgttgataaa gtttattgcg ttacctcgca aatgggtttt gaggcgcttt
      901 tgtgcggcaa accgctgacc actttcggcc tgccgtggta tgccggatgg ggtgtaagcg
      961 acgaccgcca tcctgaaatc aaccgccttg ttcaaaccca acgccgcgcc acccgcaact
     1021 tgctgcagct cttcgccgca gcctatctgc aatacagccg ctacctcaac cccaataccg
     1081 gcgaagcagg cagcctcttt gatgtcatcg actatctggc gacggtcaaa cgtaaaaacg
     1141 acaaattgcg tggcgagtta tattgcgtcg gtatgtcttt gtggaaacgc gcggttgcca
     1201 aaccgttctt taacgtaccc tcttgccgtc tgaaatttat ctcttccacc caaaaactgg
     1261 caagggtcaa actgtccgac gatgcacgca tcctggcttg gggcaacggc aaagaggcca
     1321 tcgtccgctt tgccgaacaa caccacatcc ccctgctgcg catggaagac ggctttatcc
     1381 gctcggtcgg actcggctcc aacttagtgc cgccgctgtc gctcgttacc gacgatatga
     1441 gcatttattt caatgccgaa accccgtccc gtcttgaata catcctacaa aaccaaaact
     1501 tcgacgatca agactttcag acggccttga agctgcaaaa aatgctgacc gaaaaccaca
     1561 tcagtaaata caacgtcggc agctcagact tcaccgcccc gtcaaccgac aaaaccgtga
     1621 tcctcgttcc cggccaggtt gaagatgatg cgtctatccg ctacggttcg ccccaaatct
     1681 accgcaatct ggatttgctc cgtaccgtac gcgaacgaaa ccccaatgcc tatatcatct
     1741 acaaaccgca tcccgatgta gtcagcggta accgcatcgg ccatatttcc cctgaagatg
     1801 ctgcacgata tgccgaccaa accgccgaac aagccgacat cctgacctgt ctccaatacg
     1861 cagacgaaat acataccatg acttcgctga ccggttttga agccttgttg cgcggcaaaa
     1921 aagtcagctg ctacggcctg cctttttacg caggctgggg gcttacccaa gatctgctcc
     1981 ccatcccgcg ccgtagccgc agacttgagc tttggcagct gattgccggc acgctcatcc
     2041 actatcccga ctacatccac cccgaaaccc atcaggccat aaatgcagaa accgcagccc
     2101 aaatcctgat acgacaaaaa aatatgcaaa aaaacaacaa cggattacat cgcgggtgct
     2161 ttgccaaaaa attaggtaaa atcaaacaac tatatcgatc tttcaaataa ataccatcaa
     2221 agttaacgat gcgtcataaa cttgcctcta ttgcggcatc attgcctttg catcgttaat
     2281 tctcttggcg tatgcttgaa agttcaacct aaaactatta cataaaaaac aaaaccacat
     2341 tgcaacatga aacagaccgt cctcaaaaat aacctgcaaa acctgcttga aagcgcagaa
     2401 aatatcctgc tgcttcaagg ccctgtcggc gatttttttc tgcgccttgc cgactggctg
     2461 actgcaaacg gcaaaaccgt acataaattc aactttaatg caggcgacga ctatttttat
     2521 ccgcccactc aagcgcatac cgttgttttt aacgacaact acgatgcctt tcctgagttt
     2581 ttgcaagaat acatcactca acatcacatc caggccgttg tctgctttgg cgacacacgc
     2641 ccttatcacg tcattgcaaa acgcattgca aacgaaaacc aagccagttt ctgggcgttt
     2701 gaagaaggct atttccgccc ctactacatc accttagaaa aagacggcgt caacgcattt
     2761 tccccgttgc cgcgccgtgc cgactttttt cttgaacaat tccctaagct tgcccagcaa
     2821 gaatataaag cgccaacgcc ggtacacggc ggttttacgc ccatggcaaa aaacgctatc
     2881 cgttactata tcgagttgtt ccgcaatcca cgcaaatacc ccgactacat ccaccaccgc
     2941 gcacccaatg ccggccatta cctcaaaccg tggtcgctct ccatcctcaa gcgtttgaac
     3001 tactatattg aagacatcca aatcgccaaa cgtgtggaag caggcaaata cggcaagttt
     3061 tttattgttc ccttacaggt attcaacgac agccaagtcc gtatccattg cgactttccc
     3121 agcgtccgca gcttcctgct ccatgttttg agttcatttg ccgagcacgc gcctgccgat
     3181 accaacatca tcatcaagca tcatccgatg gaccgcggtt ttatcgacta ctggcgcgac
     3241 attaaacgct ttatcaaaga acaccccgaa ctcaaaggcc gtgtgattta tgtccatgat
     3301 gtccccctgc ccgttttcct gcgccacggt ctcggcatgg tcaccatcaa cagcaccagc
     3361 ggcctgtccg gactgattca caatatgcca gttaaggttc tcggccgtgc ctattatgat
     3421 attcccggca ttactgacca aaataccttg gcagaatttt ggaatcatcc gacaccgcct
     3481 gacaaagagc tgttccatgc ctaccgaatg taccacctca acgtgaccca aattaacggc
     3541 aacttctaca gtcaggtgtt tttccccaac aaaaaaacct ccaactcttc cacaccagta
     3601 atctgactta gcgaaggaag ttcaggccgt ctgaaaacat ttcagacggc ctgaaacaat
     3661 caatacctta gctactgcca tgtaaataaa acacaaaaat ctgcatttat cattaacaat
     3721 aaattacaaa aacagtataa tgaccgagct gccatgagcg cataccgact caacctgagc
     3781 cctttgtaac acacaaaata tggatatatc cctaggcaaa acaatataac aagccaaaca
     3841 tcctaaagat aagccggcaa ggcaatacac tctataaaac tatgccgagc aaaattttta
     3901 caaagccctc aaccggtatc gccgcccata tgccgcagca tccgtcttcc actttatatc
     3961 cgcccgcaaa ccatgaccgc cgctcctgat atcctctacc ggcaagccgc cgcccttttg
     4021 gaacaatcca ataccgccca agccctgccc ctgttgcaac aggcggcaga gcaaggttat
     4081 gcggaagctg ctttcgtatt gggcaaccat ctgctgcaaa acggccaacc ggagcaggca
     4141 ctttcatggt tggaagccgc cgcggcccaa cgccatccca aagcactctt ctccctgctg
     4201 caacaacgcg aacacaacgg caccccgacc ggacagcttc tcaacgacta tgcctggctg
     4261 ggtgagcagg ggcactcaga agcccaatta atcctcatgc gttaccacgc gcaacgcaac
     4321 gatccacaat cgctctactg ggcggaactt gctgccgccc gatatgccgc acctgcgtat
     4381 taccatctgg cacgccatca tcaacgccaa ggcgacgttg aaacagccat cgaacaatac
     4441 gaaaaagcgg cagcactcgg cgtaactgcc gcctgctggc aacttggtca aatctacttc
     4501 tacggtacag gtgtcagccc caaccacgca caagccgaac actatctcga accagccgca
     4561 caagccggcc acatcgccgc acaaacgctg ctggctgacc ttcttgccgc ccaacgcaaa
     4621 cctgaagcct tggaatggta tcgtcgtgcc gccgataagg aacaagcgga agcacagtct
     4681 aagctggccc aatacgccct gaccggcgaa ctttccgaac gcgatccgtt ccaagcggca
     4741 cgatatgcca aagccgctgc cgagaaaaac catcctgaag ccctgaaaat catgggcgac
     4801 ctctaccgct acggtctcgg tatcaaagcc gacaaccata tcgcgcaaga ttactaccac
     4861 cgtgccgccg cgctgggttc tgccgccgca gcacaaaaac tcatcagcga cgccgcgctg
     4921 taccatccgc aacaatacga acaaatcaaa actgccgccc tgcaacaaca acaaaccgaa
     4981 accatctacc gtttggcgga agcacaagcc tgcgccatcg gccgtcccgc cgactacaat
     5041 gccgcgcgaa aaaattacat ggaagctgcc gggttccacc ataaaaacgc agcggcagcc
     5101 ttaggccgca tctaccatta cggcctcggt acggcgcaag atcctcgggc ggctgcacac
     5161 tggtacgcca ttgctgccga acaaaaccac ccttccgccc aataccacct cgcctgtttt
     5221 tactatcacg ggcaaggtgt cggctgtcat gttccgaccg cctgctactg gctgcaggcc
     5281 gccatcggca acggccacac ttcggccgaa tcattaatat ccctattaga acaatggcga
     5341 cgcgaagcac accatgccat cggacaaaag gccgtctgaa aagatttaca ctcgcatttt
     5401 ttgacaatct ttaactattc ccctaatatt tgccagttat ttttcacgga cacgccattg
     5461 ttttcatttc tttctgaaaa caccttgtcc gcgcatcaat accatgacac tcggcggata
     5521 acgccaagcg ttgaaacaca ctacatccgg aacaaaaacg gatgctcgga aaaatatttc
     5581 taggaggtga aacaacatgg aatgggaatt caacagttat tacacactga ttgccgccac
     5641 gctcgtgttg ctggttggta aatttctggt tcaaaaaatc aaattcttac gagacttcaa
     5701 tattcccgag ccggtagccg gcggtttgat tgccgctatc gtcctgttcg ccctgcacga
     5761 ggcgtacggc gtgagcttca aatttgagaa accgctgcaa aatgcgttta tgctgatttt
     5821 tttcacgtcc atcggcttga gcgcggattt ttcccgtttg aaggcgggcg gtttgccgct
     5881 ggtggttttt accgcgattg tgggcggatt tatcttggtg caaaactttg tcggggtcgg
     5941 actggctacg gctttgggtt tggatccgct catcggtctg attaccggtt cggtgtcgct
     6001 gacgggcgga cacggtacgt caggtgcgtg gggacctaat tttgaaacgc aatacggctt
     6061 ggtcggcgca accggtttgg gtattgcatc ggctactttc gggctggtgt tcggcggcct
     6121 gatcggcggg ccggttgcgc gccgcctgat caacaaaatg ggccgcaaac cggttgaaaa
     6181 caaaaaacag gatcaggacg acaacgcgga cgacgtgttc gagcaggcaa aacgcacccg
     6241 cctgattacg gcggaatctg ccgttgaaac gcttgccatg tttgccgcgt gtttggcgtt
     6301 tgccgagatt atggacggct tcgacaaaga atatctgttc gacctgccca aattcgtgtg
     6361 gtgtctgttt ggcggcgtgg tcatccgcaa catcctcact gccgcattca aggtcaatat
     6421 gttcgaccgc gccatcgatg tgttcggcaa tgcttcgctt tcgcttttct tggcaatggc
     6481 gttgctgaat ttgaaactgt gggagctgac cggtttggcg gggcctgtaa ccgtgattct
     6541 tgccgtacaa accgtggtga tggttttgta cgcgactttt gttacctatg tctttatggg
     6601 gcgcgactat gatgcggcag tattggctgc cggccattgc ggtttcggct tgggtgcaac
     6661 gccgacggcg gtggcaaata tgcagtccgt cacgcatact ttcggcgcgt cgcataaggc
     6721 gtttttgatt gtgcctatgg tcggcgcgtt cttcgtcgat ttgattaatg ccgcgattct
     6781 caccggtttt gtgaatttct ttaaaggctg attttccgcc tttccgacaa agcacctgca
     6841 aggtttaccg cctgcaggtg cttttgctat gatagccgct atcggtctgc accgtttgga
     6901 aggaacatca tgtatcggaa actcattgcg ctgccgtttg ccctgctgct tgccgcttgc
     6961 ggcagggaag aaccgcccaa ggcattggaa tgcgccaacc ccgccgtgtt gcaaggcata
     7021 cgcggcaata ttcaggaaac gctcacgcag gaagcgcgtt ctttcgcgcg cgaagacggc
     7081 aggcagtttg tcgatgccga caaaattatc gccgccgcct acggtttggc gttttctttg
     7141 gaacacgctt cggaaacgca ggaaggcggg cgcacgttct gtatcgccga tttgaacatt
     7201 accgtgccgt ctgaaacgct tgccgatgcc aaggcaaaca gccccctgtt gtacggggaa
     7261 actgctttgt cggatattgt gcggcagaag acgggcggca atgtcgagtt taaagacggc
     7321 gtattgacgg cagccgtccg cttcctgccc gtcaaagacg gtcagacggc atttgtcgac
     7381 aacacggtcg gtatggcggc gcaaacgctg tctgccgcgc tgctgcctta cggcgtgaag
     7441 agcatcgtga tgatagacgg caaggcggtg aaaaaagaag acgcggtcag gattttgagc
     7501 ggaaaagccc gtgaagaaga accgtccaaa cccacgcccg aagacatttt ggaacacaat
     7561 gccgccggcg gcgatgcggg cgtaccccaa gccgcagaag gcgcgcccga accggaaatc
     7621 ctgcatcctg acgacggcga gcgtgccgat accgttaccg tatcacgggg cgaagtggaa
     7681 gaggcgcgcg tacaaaacca gcgtgcggaa tccgaaatta ccaaactttg gggaggactc
     7741 gataccgacg tgcaaaaaga gttggtcggc gaacaacgca agtgggcgca ggaaaaaatc
     7801 agcaactgcc gacaagccgc cgcgcaggca gaccggcagg aatacgccga atacctcaag
     7861 ctgcaatgcg acacgcggat gacgcgcgaa cggatacagt atcttcgcgg ctattccatc
     7921 gattaggggc aaaccgatga ataccgtccc aaaaagcagg attcccgtca aaccgctgcc
     7981 cgaaaaaacc acagacgaag ccaaagtcga aaaatggcgg cagctcggtg cggaacacgg
     8041 tttgtcgggc gaatgggcag ttgccgtcag attgggcgaa aacggtttta ccgaagaaca
     8101 gatggaaaat atcgccaacc tgttcggcag ataaagagaa aattgacgga aatgccgtct
     8161 gaaaccctgt tatcggtttc agacggcatt ttgaccaata cggtacgcag gcgcaaaaca
     8221 gccggctttt cctgtgttgc ctatgctgat gtttcaacac acaggacgat acaaaaaacg
     8281 tcgccctatg tgccgtcctg attcggaagg gttacgctcc ttccaaatat agtggattaa
     8341 caaaaaccgg tacggcgtt
//
LOCUS       DAWIVB010000083         8246 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWIVB010000083 DAWIVB010000000
VERSION     DAWIVB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.083"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(147..1079)
                     /gene="cysK"
                     /locus_tag="WIX60_001263"
     CDS             complement(147..1079)
                     /gene="cysK"
                     /locus_tag="WIX60_001263"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ5305529.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1273..1878)
                     /locus_tag="WIX60_001264"
     CDS             complement(1273..1878)
                     /locus_tag="WIX60_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305530.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1875..2726)
                     /gene="dapF"
                     /locus_tag="WIX60_001265"
     CDS             complement(1875..2726)
                     /gene="dapF"
                     /locus_tag="WIX60_001265"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ5305531.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAEWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2863..3159
                     /locus_tag="WIX60_001266"
     CDS             2863..3159
                     /locus_tag="WIX60_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305532.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3173..3841
                     /locus_tag="WIX60_001267"
     CDS             3173..3841
                     /locus_tag="WIX60_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ5305533.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3914..6037)
                     /gene="pnp"
                     /locus_tag="WIX60_001268"
     CDS             complement(3914..6037)
                     /gene="pnp"
                     /locus_tag="WIX60_001268"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ5305534.1"
                     /translation="MMFDKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVA
                     VTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNG
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWS
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTS
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKI
                     REVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6271..7134)
                     /locus_tag="WIX60_001269"
     CDS             complement(6271..7134)
                     /locus_tag="WIX60_001269"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ5305535.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7333..8196
                     /gene="rfbD"
                     /locus_tag="WIX60_001270"
     CDS             7333..8196
                     /gene="rfbD"
                     /locus_tag="WIX60_001270"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ5305536.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALSAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1986 a         2379 c         1904 g         1977 t
ORIGIN      
        1 atttccctca agcgatgcgg cgacaccaac acctgattaa aacacaaccg gcgttttcgg
       61 catatatgca aaaacgcatt ttccggcacg gcgttacacc gataccgaaa aatgcgtctg
      121 aatgtttcga caatccgatt aaagcattat gccaaatctg caaaaagtgg cgtagagaga
      181 tagcgttcgc cataagaagg cagcagcacg actatcagct tgccttcgtt ttcaggctgt
      241 ttggcaagct gcaacgcact ccaaaccgcc gcaccggaag aaatacccac caaaatgcct
      301 tctttttccg ctattgcgcg ggcggtttca aaagccgctt cgttcgacac tttggtaatg
      361 ctgtcgtaaa ttttggtatt caaaacggtc ggaataaagc ctgcgccgat gccttgaatc
      421 gggtgcgggc ctttttcgcc gccgctcaat acgggtgaag cttcaggctc gacggcaacc
      481 actttaactt cgggtttgta ttttttcaac acttcgccca cgccggtaat cgtaccgccc
      541 gtgccgacgc cggcaacgaa gacatcgact ttgccgtccg tatcccgcca aatttcctcg
      601 gcggttgttt tgcggtggac ttcggggttt gcctcattgt cgaactggcg cggcataaaa
      661 taagtgtcgg gatgcgcgtc caccaaggat ttcgctttgg caatcgcgcc cgccatacct
      721 tcggcggcag gggtcagaat cagctccgca ccaaacgcgc gcaacagcat tttgcgctcc
      781 ttactcatgc tttccggcat ggtaatcgcc agcttgtagc cgcgtgcggc acataccatt
      841 gccaaaccga cacccgtatt gccgctggtt gcttcgacaa tgacggtgtt tttgttgatt
      901 ttgcccgctt tttcggcacc ctcaatcatt gcttcggcaa tgcggtcttt gacgctgctg
      961 cccggattga aaaattccag tttcacggca acctctgcct tcaaaccttc ggtcagacgg
     1021 ttcagtttga ccaaaggcgt gttgccgatt agttcggtaa tgctgtttgc aattttcatc
     1081 ttctgctcct gaataataag gtttcgacag taaggcgcaa tccccatagg catcctgtcc
     1141 gattgcaggg gatttgcgcc gaaacataaa caaatttcgg atatggcttt atcctaaacc
     1201 tatgccgcct aaaaaaccaa tatccaaaat ttctttcttt ataacttttt gttccgatac
     1261 ggccgcaccg tattatcttc caatttctga aagcagcagc accagtaccg agacctgcat
     1321 caacgctgcc gtcaacccgc cgagtcgcag ctgcgagccg ccgaacgttg acggcggctg
     1381 ccggtctata ccgcagcggt ggacataggc aaccgccatc agcacaaact gcaacacgaa
     1441 ccaataaacc ggcttcagcg cataaagttt gagcagtgcc gcaaacacaa acacttgaac
     1501 cgttcccgaa aagcaataat gcaccaacgc acatattccg acaaaagcaa gcgtcatcga
     1561 tacattactg acggcaaaaa gcccgagcag cggatgttcg gggtctgcct gccatccgtt
     1621 tccatctgtt ttccggttcc aatgcccgat gaaaaaaaat atgctgccca aagtcaggta
     1681 aaaatggggg atgaacaagg gcgcggcgcg ggtcattccc catatgccgg gcatcagctt
     1741 tgcccccaaa accgatatgc ccagccacag cataatactc gcccaaaacc attgcaacat
     1801 tcctgcgcgg aatatcagaa acagaaaaac cgccgcatac agtaaggcag acaaaaaatc
     1861 caaatgcagc aaaatcatga atacgccaac tcaccttcaa acaccgcttc cgcagggccg
     1921 gtcatcatca catcgccgcc gcaggcccat tcgatatata aagtcccgcc cggcaaaacc
     1981 acctctaccg ttttcccttc atccaacagc cccagacgga tacccgccac cacagccgca
     2041 cacgcgcccg taccgcaagc ttgggtttcg cccacgccgc gctcgaacac gcgcaaacga
     2101 atcgcggttc ggccgacaac ctgcataaag ccgacattga cgcgttcggg aaactgcctg
     2161 tgcggttcga taagcgaacc ggtttcgcgc accggcgcgc attccacgtc atcgaccaca
     2221 atcaccgcat gggggttgcc catattgacg cagctgacag gctgaatgcc ggattcgaga
     2281 tgcaccccgt aaatacaggc atcatccccc tcgcccgatt cggggacaaa cggtatttca
     2341 gacggcataa acttcggttt gcccatattg accgtaacca taccgttatc ggacaatttc
     2401 ggaaaaataa cgccatttgc cgtttcaaca caaatttctt tcttatcggt caaacccttg
     2461 tctgcaacaa aacgggcaaa acaacgcgct ccgttgccgc attgcccgac ctcgctgccg
     2521 tcggcattga aaatacggta acggaaatcc acgccttcgg tttccgaacg cccgaccacc
     2581 aaaagctggt cgaagcccac gccccggaag cggtccgccc attccgcaat cggcgcgtcc
     2641 tcgggggtaa aatcctgact gaccgcgtca atcaccataa aatcgttgcc caaaccgtgc
     2701 attttggtaa atttcaaagt gttcatattg ttccgaaatc ccgtatcgtc tgttgcttcc
     2761 gaatccgaaa tcccccgcac cgaagggaaa tgcttccatc tcccgccgcc tgaggattaa
     2821 aatcagccat tatactgttt gttacccctt acaagaaaca ccatgttccg catattactg
     2881 ttgcttttcc cttgtgcaat ccttgccgcc tgcacccgaa acataccggc aaacccggcc
     2941 gtttcaaatt gcgcgcatct tcaaaaccgc accatcctgt attttgacgc acacggcaaa
     3001 cccgccgacg gcgcagtaaa gggcggattt tacaaaatta tcgaaataca gggtgcaaac
     3061 tgcatcctta ttcaagactt taacagcacg ggcaatgccc tcggcggatc ctatgtgccg
     3121 gactgccgca aatcatccct tttcccgaaa aatatttgac ttcaaactgc ctatgaacca
     3181 cgacatcact ttcctcaccc tgttcctact cggtttcttc ggcggaacgc actgcatcgg
     3241 tatgtgcggc ggattaagca gcgcgtttgc gctccaactc cccccgcata tcaaccgctt
     3301 ttggctgatc ctgctgctta acacaggacg ggtaagcagc tatacggcaa tcggcctgat
     3361 actcggatta atcggacagg tcggcgtttc actcgaccaa acccgcgtcc tgcagaatat
     3421 tttatacacg gccgccaacc tcctgctgct ctttttaggc ttatacttga gcggtatttc
     3481 ttccttggcg gcaaaaatcg agaaaatcgg caaaccgata tggcggaacc tgaacccgat
     3541 actcaaccgg ctgttaccca taaaatccat acccgcctgc cttgcggtcg gaatattatg
     3601 gggctggctg ccgtgcggac tggtttacag cgcgtcgctt tacgcgctgg gaagcggtag
     3661 tgcggcaacg ggcgggttat atatgcttgc ctttgcactg ggtacgctgc ccaatctttt
     3721 agcaatcggc attttttccc tgcaactgaa aaaaatcatg caaaaccgat atatccgcct
     3781 gtgtacggga ttatccgtat cattatgggc attatggaaa cttgccgtcc tgtggctgta
     3841 agccccattc attcccgata cccgtaaaaa ataccgtctg aaacctgttc agacggcact
     3901 ttcaccctaa gcgttactcg gcggcatttt cctcacgggc aggcgcgtcc agcagggctt
     3961 tgatggacag acggacacgg cctctgtcgt ccacttccaa tgctttcacg ttcaccacct
     4021 gaccgacttg caggtagtcg ccgacattgc gtacgcgctc gtgggcgatt tggctgatgt
     4081 gtaccaaacc gtctttgccc ggcatcacgc tgacaatcgc gccgacattg ttatcgagga
     4141 ttttcaccac agtgccttcg tacactttgc ccacttccac ttcggcagta atctgctcga
     4201 tgcgtttttt cgccgcatcg ccggcttctt gagtggttgc ggcaatggta atcgtaccgt
     4261 cttcggcaat attgatttcc gtaccggttt cagcggtaat cgaacggatg gtttcaccgc
     4321 ccttaccgat aacttcgcgg attttgtctt ggttgatttt catcgtgaac aagcgtggcg
     4381 cgtgtgcgga cagctcttgc gggcccgcaa cggcggcttt catctgatcc aagatgtgca
     4441 gacgcgcttc tttggcctgt gccaaagcga tttgcataat ttctttggta atgccttgga
     4501 ttttgatgtc catttgcagc gcggtaacgc cttcggtcgt accggccact ttaaagtcca
     4561 tatcgcccaa gtggtcttcg tcgcccaaaa tgtcggtcag gacggcaaat ttgttgcctt
     4621 ccagaatcag acccatcgcg ataccggcaa cgtgtgcttt caaaggcacg ccggcagaca
     4681 gcaggctcag gcagccgccg cagacggaag ccatagagga agagccgttg gattcggtaa
     4741 tttcggagac cacgcgcatg gtgtagctga aatcttcagg tttcggcaat acggccaaca
     4801 atgcacgttt agccaaacgg ccgtgaccga tttcacggcg tttcggtgcg cccatgcggc
     4861 ccacttcgcc ggtagagtac ggcggaaagt tgtagtgcag cataaagcgg tcggtgtatt
     4921 cgccggacag cgcgtcgatg atttgctcgt cgcgcgaagt acccaaagtt gcaacggcca
     4981 aagcttgggt ttcgccacgg gtaaacaatg cagaaccgtg cgtgcgcggc aatacgctgg
     5041 tttggatgtt cagcggacgg acggtgcggg tgtcgcggcc gtcgatgcgc ggttggccat
     5101 ccaaaatttg gctgcggacg acatcggctt ccaagtgttt gaaaatgcct ttgatttcgt
     5161 tggctgccaa agtgtcggtt tcttcggtaa tcaaggcttc ttttaccgca ctccaagctt
     5221 cgtccaattt ggcagaacgc gcttgttttt gacggatttt gaacgcttct ttaatggttt
     5281 cgccggcaat cccgcggact ttggcaacca gttcctcatt ggtttcaggt gctttccaat
     5341 cccaaagttc cggattgact tcgtcggcaa attcattgat tgcattgatg gcaacctgca
     5401 tttgatcgtg gccgtaaacc accgcgccca gcatcacgtc ttcgggcagg attttggctt
     5461 cggattccac catcaacacg gcttttgaag taccggcgac caccaagtcc aattgcgatt
     5521 tcgccaattc ggctttagtc ggattcaaaa cgtacacgcc gtttacataa ccgacgcgtg
     5581 ccgcgccgat cgggccggca aacggtacgc cgctcaacac cagcgcggca gatgcaccca
     5641 acattgcagg aatatcagaa tcgatttcag gatcgacgga cacgaccatc gctacgattt
     5701 ggatgtcgtg gtagaaacct tcagggaaca gcggacgaat cggacggtcg atcagacggc
     5761 tggtcaggat ttctttttcg ctttgtttgc cttcgcgttt gaagaaaccg ccgggaattt
     5821 tgcctgcggc gtaagtgcgt tccaaataat cgacggtcag ggggaagaag tcttgacctt
     5881 ctttcacttc tttgttggtg gtaacggcaa ccaaaacaac ggtgtcgccc atagagactt
     5941 taacggcagc ggcggcttgg cgggcaattt cgccggtttc caaagtaacg gtctgattac
     6001 cgtattggaa ggtcttaacg tgtttgtcga acatcattgt tcctttcaaa ataccgcact
     6061 gctaaaacac taataatgca cactaaaatc cgaatgtgca tagttagggt ttcagaccgt
     6121 gcggcaggtt ataaacaagc tttcagacgg catttcggac gctgaaagca attgcggatt
     6181 ataaaggcaa ccatccttaa aatccagtat tgatacaata aaaaggccgt ctgaaccata
     6241 tttctttcag acggccttca aacttaaaaa tcaatcctga gtcagcaaag tcaatgcttc
     6301 ccgatacttc tcgacagttt tctgaatcac atcggcaggc actttcggcg caggggcttt
     6361 tttgttccaa ccgctttgtt ccagccagtc gcggacgaat tgtttgtcaa aagacggcgg
     6421 attggtgccg actttgtatt ggtcggcagg ccaaaaacgg ctcgaatcgg gagtcaatac
     6481 ctcatccatc agcgtcagcg taccgtttgt atccaaaccg aattcaaatt tggtatcgca
     6541 aatgatgatg ccgcgcgatt tggcatattc cgccgcttct gtataaagct gaatcgcttt
     6601 ggcgcgcact tccgccgcca attcttttcc gataatgcgt tcgcattctt caaagctgat
     6661 gttttcatcg tgatcgccga ctgcggcttt ggttgagggc gtaaaaatca cttcaggcag
     6721 ttgttgcgct tcctgcatac cttcaggcag ttgaataccg caaaccgagc cggttttttg
     6781 ataatctttc caaccgctgc ctgccagata accacgcaca atcgcctcta ctttcaccgg
     6841 agtgagcttt ttagccacga cggcgcgttt ctctaaagct ttggcttcgt tttcaggcaa
     6901 aacatcgtaa accgtttgac cggtaaagtg gttgggcata atatgcacca gttttttaaa
     6961 ccaaaaattg gaaatctgcg tcagaatctc ccctttgctc ggaatcgggt cgtccaaaat
     7021 cacatcgaat gcagacaggc ggtcggaagc gaccatcagc atacgtttat cgtcgatttc
     7081 atataaatcg cgcacttttc caaaatagat ctttaccaaa ccaatctcac tcatttcgcc
     7141 ccccctgaaa atatcttgaa aataccgacc cgacacccga caggtttgaa tcacaaacca
     7201 atattctagc cgaagtcggc gcaaaacaat acccatggca caaaaaacca acccgtcaac
     7261 cgtcggcaaa gttttggcac tataataccg acagcaagtc ctacaataca cttttaccaa
     7321 aggaaatacc tcatgagaat cctattgaca ggctcgaaaa gccaactggc acgctgcctg
     7381 cgcgaccgtc ttccggaaga ctgggaaacc attgcgacgg attccgcatc cctagacatt
     7441 accgatgccg atgccgtctg caacatggtc aaaagtttcc aacccgacgc cattgtcaac
     7501 acggctgcct atactgccgt cgacaaagcg gaaggcgatg cggcagcggc atttgccgtc
     7561 aatgcttccg ccgtttacaa ccttgccttg tcagcacatc gcgcccatgc ccgattcatc
     7621 cacatctcaa ccgactatat ctttgacggt aaagggaaaa gaccctatca ggaaagcgac
     7681 tttaccaatc cttccaatgt atacggacaa tccaaaaccg caggcgagct gctcgcactg
     7741 tctgccaatc ccgacagcct tatcctgcgg acttcttggc tgtttagcga atacggggac
     7801 aactttgtcc gcacgatgct gaaccttgca cgggaacgtt ccccgctgtc cgccgtccac
     7861 aaccaaatcg gctgcccgac ctatgccggc gatttagctg ccgccatcat ccgccttttg
     7921 cagcaaacgt cgcccgtgcg cggcatttac cactacgccg gcggcaaatc cgtatcctgg
     7981 tacgaatttg cccaacatat tttccaagcg gcatcgcaac agcagacatc cttccccgtt
     8041 cccgaattga ctgccgtttc agacaaggaa tatccgaccg ccgcccccag gcccgcatac
     8101 agcattttgg actgccgcaa aatcgaaaac gacttcagca tcaaaccgtc agactggcaa
     8161 aaagcccttg cacaggtcgt ttccaagctg ctctgatgcc gcccgcctct ccgtttccgc
     8221 cgtcaagcac cgccttggcg gttttc
//
LOCUS       DAWIVB010000084         8222 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWIVB010000084 DAWIVB010000000
VERSION     DAWIVB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.084"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(332..748)
                     /gene="dksA"
                     /locus_tag="WIX60_001271"
     CDS             complement(332..748)
                     /gene="dksA"
                     /locus_tag="WIX60_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ5305537.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(895..1686)
                     /locus_tag="WIX60_001272"
     CDS             complement(895..1686)
                     /locus_tag="WIX60_001272"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ5305538.1"
                     /translation="MNVYFLGGGNMAAAIAGGLVKQGGYRIYIANRGAEKRERLEKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTLSRYLGG
                     NRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1735..2142)
                     /locus_tag="WIX60_001273"
     CDS             complement(1735..2142)
                     /locus_tag="WIX60_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ5305539.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCQLAGWYECSSLSGWCKLRKPAA
                     IDFWDIGGESPPSLEDYEIPLLDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(2142..2828)
                     /locus_tag="WIX60_001274"
     CDS             complement(2142..2828)
                     /locus_tag="WIX60_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ5305540.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRDPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTIC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVVLAVEVAKLPNIVVRG
                     LMCVAKAGGSEAELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2948..3991
                     /locus_tag="WIX60_001275"
     CDS             2948..3991
                     /locus_tag="WIX60_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ5305541.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            4154..5380
                     /locus_tag="WIX60_001276"
     CDS             4154..5380
                     /locus_tag="WIX60_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ5305542.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5666..7816
                     /gene="yccS"
                     /locus_tag="WIX60_001277"
     CDS             5666..7816
                     /gene="yccS"
                     /locus_tag="WIX60_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ5305543.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         2049 a         2678 c         1761 g         1734 t
ORIGIN      
        1 acaggacaat gttcaaaaca ttatcttgtc tgtctttcaa acagtgtgag actcaaggca
       61 ctcacactta tcggtaatct gttttgttaa agagcgtttg aactgcttca tcaccgccgc
      121 ttcgtcagca gcgaagaaac gaactatacg cttgaagacg tatacggtca atacttcatc
      181 ttgctttttt taattatttt ctccatctcg ttgtctgata aggtttttta tttcgccaca
      241 aaccacatcc gactcgaaat ttacctgccg ccatcttaat ctgcttccct ttttttctgt
      301 catatggttt gaaaataacc aaaaggcaaa tttagtcggc aaactgtttt ttcatcctct
      361 ctcggcgttc ttgggactca acagataaag tggctgtcgg gcgtgccagc agccgcttca
      421 gaccgatagg ctctcccgta tcggcacaga atccataatc cccttcatca atattgcgga
      481 tggtcgcctg tattttactg agaagttttc gttcccgatc gcgggtacgg agttccaatg
      541 cgtactcttc ttcctgtgtg gcacggtcgg caggatcggg ggctgattcg tgttcttgga
      601 gatgccctgt cgtagcggaa gcattttcga tgagttcgtc ttgcattttt accagcaatt
      661 cgcggaaaaa agccaaatgc tcgtcattca tataatcgtc ttccggcccg ttccaattca
      721 aaatatcttg ttctgtcagc tttgccataa tgtatatcct ctcggttcac cgaaaaaaac
      781 aaagcaccac aaagtggctt tattcctttg atttttataa ggcttattgc ctaaggcgtt
      841 tttgaaaacg cgcatcataa cacgttttgg attggttttt ttattttctt tacattattg
      901 atattgccgt tccatttcct gcgaacggcg cacacaggca caaacgccct cacttatggc
      961 ttcggcgaca cgatgccgcc tgaaggcttc cacggcttcg tgggttgtcc cgccttttga
     1021 cgttacattt ttttgaagct tctcgaaatc ttcacccgtc tgctcggcaa gggcaaccgc
     1081 tcctttaaac gttgccagac tgagcgcgcg tgcttctgcc atatcaaacc cttgtcggat
     1141 ggcggcattt tgcaatgcgt ccagcagata aaacacatat gccggcccgc tgccgctgat
     1201 accggtaatg ctgtgcattt gcgcttcatc ttccaaccaa acggtcaaac cgactgattt
     1261 catgattcga tcggcaatcc tgcggtctgt ttccgatact tccgcttcgg caaacatacc
     1321 ggatacgccc agcccgattt ttccgggtgt attcggcata acccggacaa tgcggcggtt
     1381 tcccccgagg taacggctga gcgtatcaac cgacaatcca gctgcaacag aaagcaccaa
     1441 tgcgccgttg gtgcggatat ttttgcacgc ggcttccata tcctgcggtt tgacggcaag
     1501 gattaaaaca tcgtcggaat gaagctccgg cagggttgcc gaagtttcga cccccaactc
     1561 tttttccaaa cgttcgcgtt tttccgcacc ccgattggct atatagatgc ggtaaccgcc
     1621 ttgtttgacc aatccgcctg cgatggcagc cgccatattg ccgccgccga gaaaataaac
     1681 attcattatt tctaccctat tcaaaattac cttactaaat tcattcccaa ttttttacca
     1741 tcggacacgc tcggacagac cgttgcgccg caacccctgc tttttcaaac aatcaaaacc
     1801 ttcctgtttg aacgtctcaa tcaaaggttt gccgtcacgc gtacgccaat ccaacccgca
     1861 ggcttcaaac ttccctattt tcctgtaaaa gtaagattgt tgtgcggatt catattcgtt
     1921 tgccctgacg gaacgattgc cgtctaaaag cggtatctcg tagtcctcta aagacggcgg
     1981 actctcgccg ccaatgtccc aaaaatcgat ggcagccggt tttctcagct tacaccagcc
     2041 ggacagggac gaacactcat accaacccgc caactggcag cctgcaaccg taaatgcaag
     2101 caacgcagcc attgccgtat attttattgc ccgaatttcc atcagcccct tttcccgaaa
     2161 atcgcgctgc cgatacggac gtgtgtcgca ccgcactcaa tggcggcagg catatcgtcc
     2221 gacatcccca tagacagcac gtccgcctta acgccagccg tattgaggtc ggcaagcaac
     2281 ttttgcatgg tctgaaactg ggttttcaat tctgcctcgc tgccgccggc tttggcaaca
     2341 cacatcagtc cgcgtacgac gatattcggc aacttcgcca cttcgaccgc aagcacgacc
     2401 gcttcttcgg gcgcgacacc gtgtttcgcc gcctcgcccg caatgttcac ctcgatacac
     2461 acctgcaaag gcggcattga ggaaggacgt tgcccgctca gccggacggc ggtctttaga
     2521 cggcatatgg tatgcaccca atgcgcgcgt tcggcgacaa acttggtttt gttggactgc
     2581 acatcgccga tgacgtgcca cacgatgtcg gtcaaatccg ccaactcttc cgttttgccg
     2641 taccactcct gaatatagtt ctcgccgaaa tcacgctgtc cggcggcgta aacttcgcgg
     2701 atgccgtctg aagggaaagt cttaccgacg gcaatcaggc tgacggaatg cggatccctg
     2761 cccgcctgca gaaccaattt tccgatacgg tcggacacct cacaataacg ttcttgcaac
     2821 accgtcatag attatcccct aattaaaaat gattaaacag ttgaaacccc tccagtcagg
     2881 gcggtacaat caaggttgtt agaaccattc caaccaatcg aaacattata ctaaacagag
     2941 ccgcattatg cagattaccg acttactcgc cttcggcgct aaaaacaaag catccgacct
     3001 tcacctgagt tcgggcatat cccctatgat tcgggttcac ggcgatatgc ggcgcatcaa
     3061 ccttcccgaa atgagcgcgg aagaggtcgg taatatggta acttcggtga tgaacgacca
     3121 ccagcggaaa atctaccagc aaaacttgga agtcgacttc tcgttcgaac tgcccaacgt
     3181 cgcccgattc cgcgtcaacg ccttcaacat cggccgcggt cccgccgccg tattccgcac
     3241 cattcccagc accgtcttat cgctggaaga attgaaagcc ccgagcattt tccaaaaaat
     3301 cgcagaatcg ccgcgcggca tggttttggt taccggccct accggttcgg gcaaatcgac
     3361 cacgcttgcc gcaatgatca actacatcaa cgaaacccag ccggcacaca tcctgaccat
     3421 cgaagacccg atcgaattcg tccaccaaag caaaaaatcc ctgattaacc agcgcgagct
     3481 gcaccagcac accctcagct tcgccaacgc gctgcgttcc gcattgcgcg aagaccccga
     3541 cgttatcctt gtcggcgaga tgcgcgaccc agaaaccatc ggcttggcgc tgaccgccgc
     3601 cgaaaccgga cacttggttt tcggcacgct gcacacgacc ggcgcagcaa aaaccgtcga
     3661 ccgtatcgtg gacgtattcc cggcgggaga aaaagaaatg gtgcgctcta tgctgtccga
     3721 atcgctgacc gccgtcatct cccaaaacct gctgaaaacg cacgacggca acggccgtgt
     3781 cgcctcgcac gaaatcctga ttgccaaccc cgccgtccgc aacctcatcc gcgaaaacaa
     3841 aatcacgcag attaactccg tcctgcaaac cgggcaggcg agcggtatgc agacaatgga
     3901 ccaatcgctg caatcgctgg tgcgccaagg gctgatcgca ccggaagtcg cacgcagacg
     3961 cgcgcaaaac agcgaaagta tgagtttctg acacacaacc gccttccggc cataccggcg
     4021 ggaaaacaag gcgcaaacac gcgaggcggg acgcagcatc ccgcccggct acctttccga
     4081 acaagaagcg tccgccttcc tgttgaaacc tgccgccgca aactgcaagg cttaaaccga
     4141 aaagaagcta acgatgaata ccgataacct gcacgacatc ttggacgaaa tggttcaagt
     4201 gtattctcaa aaaaaacaaa gccgatccga aaccccggcc gaaatcggcg cacacttcca
     4261 cccgctgctc gaccgcctgt gcgaaaccgc agaagcacaa aacgcgtccg acatccttat
     4321 cagcaaagga ttcccgccct cgttgaaaat caacagcgca ttaaccccgc agccgcaaaa
     4381 ggcgctgaca ggcgaggaaa ccgccgccat cgccgcatcg acgatgaacg ccgaacaatc
     4441 ggaaatattc cggcgcgacg gcgaaatcaa ctactccgtc cagtcgcgca gcggcacgcg
     4501 ctaccgcgcc aacgcctacc acagccaagg cagcgcaggt ttggttttgc ggcgcatcaa
     4561 ccacgtcatc ccgcaaatgc aggaattggg cctgcccgaa aaactcaaag acctcgccgt
     4621 cgcaccgcgc gggctgctga ttatcgtcgg gcctaccggt tcgggcaaat ccaccacgat
     4681 ggcgactatg ctcgaacacc gcaacaaaac cctgcccagc catatcgtta ccatcgaaga
     4741 cccgattgag tttatctaca aaccgcgccg ctgcatcttt acccagcgcg aaatcggcgt
     4801 cgacaccata aactggcaga cggcggtaca aaacgctatg cgccaatccc ccgacgtggt
     4861 ctgcatcggc gaagtccgca gcagggaaag tatggaatac gcgatgcagc tcgcccaaac
     4921 cggccacctg tgcattttta cgctccacgc caacaccgcg ccgcagtcgc tcgaacgcat
     4981 actcaacttc taccccaaag aacagcacaa ccaaatactg atcgacatcg ccctcaacct
     5041 gaccggcatc atctgccaac gcctcgccct caaacaagac aaaacgggca ggacggcggt
     5101 tgtcgacttg ctcatcaaca cgcccgccat ccaagacttc atcctgaagg gcgacctgat
     5161 gaacatcagt aaaatcatgg aaaccgccaa aaccgacgga atgcagacga tggatcaaaa
     5221 ccttttcgaa ctgtaccgtc acggcatcat cagttacgaa gaagccctgc gccagtccgt
     5281 ttccgccaac aacctgcgat tgcacatcca actgcacaaa gaaggcaaaa cgcccgaact
     5341 cctttacgac agggtcaacg gtctcaacct catttcctga tccgcaaaac ccaatgccgt
     5401 ctgaaaaccg catccccgtt ttcagacggc atgattttat ccgccccatt catgtgctac
     5461 actttatagt ggattaaatt tatagtggat taacaaaaat caggacaaga cgacgaagcc
     5521 gcagacagca caaatagtac ggaaccgatt cacttggtgc ttcagcacct tagagaatcg
     5581 ttctctttca gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctatattcac
     5641 ttcataacaa taaaccggta aaaccatgaa aaccccactc ctcaagcctc tgctcattac
     5701 ctcgcttccc gttttcgcca gtgtttttac cgccgcctcc atcgtctggc agctaggcga
     5761 acccaagctc gccatgccct tcgtactcgg catcatcgcc ggcggccttg tcgatttgga
     5821 caaccgcctg accggacggc tgaaaaacat catcgccacc gtcgccctgt ttaccctctc
     5881 ctcgctcacg gcacaaagta ccctcggcac agggctgccc ttcatcctcg ccatgaccct
     5941 gatgaccttc ggctttacca ttttaggcgc ggtcgggctg aaataccgca ccttcgcctt
     6001 cggcgcactc gccgtcgcca cctacaccac acttacctac acccccgaaa cctactggct
     6061 gaccaacccc ttcatgattt tgtgcggcac cgtactgtac agcaccgcca tcatcctgtt
     6121 ccaaatcatc ctgccccacc gccccgtcca agaaagcgtc gccaacgcct acgacgcact
     6181 cggtggctac ctcgaagcca aagccgactt cttcgacccc gatgaggcag cctggatagg
     6241 caaccgccac atcgacctcg ccatgagcaa caccggcgtc atcaccgcct tcaaccaatg
     6301 ccgttccgcc ctgttttacc gtttgcgcgg caaacaccgc cacccgcgca ccgccaaaat
     6361 gctgcgctac tacttcgccg cccaagacat acacgaacgt atcagctccg cccacgtcga
     6421 ctaccaagag atgtccgaaa aattcaaaaa caccgacatc atcttccgca tccaccgcct
     6481 gctcgaaatg cagggacaag cctgccgcaa caccgcccaa gccctgcgcg caagcaaaga
     6541 ctacgtttac agcaaacgcc tcggccgcgc catcgaaggc tgccgccagt cgctgcgcct
     6601 cctttcagac agcaacgaca atcccgacat ccgccacctg cgccgccttc tcgacaacct
     6661 cggcagcgtc gaccagcagt tccgccaact ccgacacagc gactcccccg ccgaaaacga
     6721 ccgcatgggc gacacccgca tcgccgccct cgaaaccggc agcctcaaaa acacctggca
     6781 ggcaatccgt ccgcagctaa acctcgaatc aggcgtattc cgccatgccg tccgcctgtc
     6841 ccttgtcgtt gccgccgcct gcaccatcgt cgaagccctc aacctcaacc tcggctactg
     6901 gatactgctg accgcccttt tcgtctgcca gcctaactac accgccacca aaagccgcgt
     6961 ccgccagcgc attgccggta ccgtactcgg cgtaatcgtc ggctcgctcg tcccctactt
     7021 caccccgtct gtcgaaacca aactctggat tgtcatcgcc agtaccaccc tctttttcat
     7081 gacccgcacc tacaaataca gtttctccac cttcttcatt accattcaag ccctgaccag
     7141 cctctccctc gcaggtttgg acgtatacgc cgccatgccc gtacgcatca tcgacaccat
     7201 tatcggcgca tcccttgcct gggcggcggt cagctacctg tggcccgact ggaaatacct
     7261 cacgctcgaa cgcaccgccg cccttgccgt atgcagcaac ggtgcctatc tcgaaaaaat
     7321 caccgaacgc ctcaaaagcg gcgaaacagg tgacgacgtc gaataccgcg ccacccgccg
     7381 ccgcgcccac gaacacaccg ccgccctcag cagcaccctt tccgacatga gcagcgaacc
     7441 cgcaaaattc gccgacagcc tgcaacccgg ctttaccctg ctcaaaaccg gctacgccct
     7501 gaccggctac atctccgccc tcggcgcata ccgcagcgaa atgcacgaag aatgcagccc
     7561 cgactttacc gcacagttcc acctcgccgc cgaacacacc gcccacatct tccaacacct
     7621 gcccgaaacc gaacccgacg actttcagac agcactggat acactgcgcg gcgaactcga
     7681 caccctccgc acccacagca gcggaacaca aagccacatc ctcctccaac agctccaact
     7741 catcgctcgg cagctcgaac cctactaccg cgcctaccga caaattccgc acaggcagcc
     7801 ccaaaacgca gcctgaaaaa gtttcggcat tttgtaagag agagcagatt gtcagacagg
     7861 ttgcaagata gtggataaag ctttgcgcag ggtaaatgcg tagcaactga accgtcattc
     7921 ccacgaacct acatcccgtc attcccacga aaacagaaaa ccaaaaacag aaacctaaaa
     7981 tcccgtcatt cccacgaaag tgggaatcca gaatcctgga ctttcagata atctttgaat
     8041 attactgttg ttctaaggtc tagattcccg cctgcgcggg aatgacgaca gaacggtttc
     8101 tgttttttcc ggtaaattcc taaaactcaa aatttcatca ttcccacaaa aacagaaaac
     8161 caaaatcaga aacctaaaat tcgtcattcc cacgaaagtg ggaatccggt tcgttcggtt
     8221 tc
//
LOCUS       DAWIVB010000085         8217 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWIVB010000085 DAWIVB010000000
VERSION     DAWIVB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.085"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            284..1453
                     /gene="prpF"
                     /locus_tag="WIX60_001278"
     CDS             284..1453
                     /gene="prpF"
                     /locus_tag="WIX60_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ5305544.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1867..3066
                     /locus_tag="WIX60_001279"
     CDS             1867..3066
                     /locus_tag="WIX60_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ5305545.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            3425..4045
                     /locus_tag="WIX60_001280"
     CDS             3425..4045
                     /locus_tag="WIX60_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ5305546.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(4172..4615)
                     /locus_tag="WIX60_001281"
     CDS             complement(4172..4615)
                     /locus_tag="WIX60_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ5305547.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4789..5157
                     /locus_tag="WIX60_001282"
     CDS             4789..5157
                     /locus_tag="WIX60_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ5305548.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            5202..6356
                     /locus_tag="WIX60_001283"
     CDS             5202..6356
                     /locus_tag="WIX60_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ5305549.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVLAWLSAVFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6413..7285)
                     /locus_tag="WIX60_001284"
     CDS             complement(6413..7285)
                     /locus_tag="WIX60_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ5305550.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDQASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7370..8182
                     /locus_tag="WIX60_001285"
     CDS             7370..8182
                     /locus_tag="WIX60_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ5305551.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSCEAGKVMNTLL
                     VYGRDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1792 a         2232 c         2386 g         1807 t
ORIGIN      
        1 atttaatcca ctatactgta aatcgtcatt cccgcgcagg cgggaatcca gaaagtggaa
       61 ttgaggaaac ctttttatcc gatgagtttc tgtgcggata aatctggatt cccgcctgcg
      121 cgggaatgac ggggtttaat aatctgccgt atcacaacac agtagccgta gattggggcg
      181 aaccccgaca gtttgcggaa tcaaacggct ttggtcggag tggcagccta atccactata
      241 aaaatcgtgg gcagagccca cgctacataa ggagaatcta gaaatgccgc aaattaaaat
      301 tcccgccgtt tactaccgtg gcggtacatc aaaaggcgtg tttttcaaac gttccgacct
      361 gcccgaggcg gcgcgggaag cgggaagcgc acgcgacaaa atcctcttgc gcgtactcgg
      421 cagcccggat ccctacggca agcagataga cggtttgggc aacgccagct cgtccaccag
      481 caaggcggtg attttggaca agtccgaacg cgccgatcac gatgtcgatt accttttcgg
      541 gcaagtttcc atcgacaaac cttttgtcga ttggagcggc aactgcggca acctcaccgc
      601 tgccgtgggc gcattctcca tcgaacaggg cttggtcgat aaaggcaaga ttccttcaga
      661 cggcatctgc acggtcaaaa tctggcagaa aaacatcggc aaaaccatta ttgcccatgt
      721 accgatgcaa aacggcgcag ttttggaaac aggcgatttt gagctcgacg gcgtaacgtt
      781 cccggcagcc gaagtacaaa tcgaatttct tgatccagcc gacggcgaag gcagtatgtt
      841 cccaaccggc aatttggtcg atgaaattga tgtgccgaat ataggccgtt tgaaagccac
      901 gctcatcaac gcgggcattc cgaccgtttt cttgaatgcc gccgacttgg gctacacagg
      961 caaagagttg caagacgaca tcaacaacga tgccgcggct ttggaaaaat tcgagaaaat
     1021 ccgcgcttac ggtgcgctga aaatgggtct gatcagcgac gtatccgaag ctgccgctcg
     1081 cgcgcacacg ccgaaagtcg ccttcgtcgc gcccgccgcc gattacaccg cctccagtgg
     1141 caaaaccgtg aacgccgccg acatcgattt gctggtacgc gccctgagca tgggcaaact
     1201 gcaccacgcg atgatgggta ccgcctctgt tgccattgcg accgccgccg ccgtacccgg
     1261 tacgctggtc aaccttgccg caggcggcgg aacgcgtaaa gaagtgcgct tcgggcatcc
     1321 ttccggcaca ttgcgcgtcg gtgcagccgc cgaatgtcag gacggacaat ggacggccac
     1381 caaagcggtc atgagccgta gcgcacgcgt gatgatggaa ggttgggtca gggtgcctga
     1441 ggattgtttt taaattgacg tagcatgggt ttgcccgcga gccataaaaa ggtcgtctga
     1501 aaaacaagta aacatcaaat cactgaccat tcctttccct tgccctgtgg cggaaggcgg
     1561 caaatcacaa ggaagaacac ggaaaccccg ataaaagaca gcttcccgta ttaccgtcat
     1621 tcccgcgcag gcgggaatcc agacctgtca atatggagga ttggcagggg aaaacaggtt
     1681 tcgtgagttc tacattctgg attcccgcca cagcctgtcc tcgcgtaggc ggggacggaa
     1741 taacgataga aaatgcggca tacgctttgc ccaaagaggc cgtctgaaac accttgcgcc
     1801 tgatgtctgc ctttttcaga cgaccccaca ccaaaaaaac aaccacaaac tacaaggaga
     1861 aacatcatgt ccgaccaact catcctcgtt ctgaactgcg gcagttcatc gctcaaaggc
     1921 gccgttatcg accgaaaaag cggcagcgtc gtcctaagct gcctcggcga acgcctgacc
     1981 acgcccgaag ccgtcattac gttcaacaaa gacggcaaca aacgccaagt tcccctgagc
     2041 ggccgaaatt gccacgccgg cgcggtgggt atgcttttga acgaactgga aaaacacggt
     2101 ctgcacgacc gcatcaaagc catcggccac cgcatcgccc acggcggcga aaaatacagc
     2161 gagtctgttt tgatcgacca ggccgtaatg gacgaactca atgcctgcat tccgcttgcg
     2221 ccgctgcaca accccgccaa catcagcggc atccttgccg cacaggaaca tttccccggt
     2281 ctgcccaatg tcggcgtgat ggatacttcg ttccaccaaa ccatgccgga gcgtgcctac
     2341 acttatgccg tgccgcgcga gttgcgtaaa aaatacgctt tccgccgcta cggtttccac
     2401 ggcaccagta tgcgttacgt tgcccctgaa gccgcacgca tcttgggcaa acctctggaa
     2461 gacatccgca tgattattgc ccacttaggc aacggcgcat ccattaccgc catcaaaaac
     2521 ggcaaatccg tcgataccag tatgggtttc acgccgatcg aaggtttggt aatgggtaca
     2581 cgttgcggcg acatcgatcc gggcgtatac agctatctga cttcccacgc cgggatggat
     2641 gttgcccaag tggatgaaat gctgaacaaa aaatcaggtt tgctcggtat ttccgaactt
     2701 tccaacgact gccgcaccct cgaaatcgcc gccgacgaag gccacgaagg cgcgcgcctc
     2761 gccctcgaag tcatgaccta ccgcctcgcc aaatacatcg cttcgatggc tgtgggctgc
     2821 ggcggcgttg acgcactcgt gttcaccggc ggtatcggcg aaaactcgcg taatatccgt
     2881 gccaaaaccg tttcctatct tgatttcttg ggtctgcaca tcgacaccaa agccaatatg
     2941 gaaaaacgct acggcaattc gggcattatc agcccgaccg attcttctcc ggctgttttg
     3001 gttgtcccga ccaatgaaga actgatgatt gcctgcgaca ctgccgaact tgccggcatc
     3061 ttgtagccaa aaaagggacg agtccgcaaa aatgccgtct gaaaccccaa acgcccgatt
     3121 aggctgatga ggattttaga cggcattgtt catttttttg ttatcttgca tttttgtgcg
     3181 gacggtggaa tttcatcctg taaacataaa tatttgtcgg aaaacagaaa ccctccgccg
     3241 ccatttctac gaaagcagga aaccagcaac gcaaagcgac agggatttgt tggaaatgac
     3301 cgaaaccgaa cgaaccggat tcccgcctgc gcgggaatga cgggattttc tgtttttgtg
     3361 gaaatgacgg gattttgaat ttcgggcgta caatacggaa aacatgacga taaggaaaca
     3421 aaccatggca cagtttttcg ctattcatcc cgacaatccc caagaacgcc tcatcaagca
     3481 ggcggttgaa atcgtcaata aaggcggcgt ggtcgtttat ccgaccgatt cctgttatgc
     3541 cttgggctgc aaactcggcg ataaggcggc gatggaacgc atactctcca tccgcaaaat
     3601 cgatttgaaa caccacctga ccctgatgtg cgcagatttg agcgagttgg gcacatacgc
     3661 caaagtcgac aacgtacagt ttcgtcagct taaagccgcc acacccgggc cttatacttt
     3721 tattttacag gcgacgaagg atgtgccggc gcgcacgctg cacccgaaac gcaaaaccat
     3781 cgggctgcgt attcccgata atgccattgc acaagccctg ctgggggaat tgggcgagcc
     3841 gcttttaagc tgcaccctga tgctgcccga agacggcgaa ccattgaccg atccttatga
     3901 aatccgcgag cgtttggaac acgccgtcga tttggtgatt gacggcggct ggtgcggaac
     3961 cgagccgacc accgtcgtcg atatgaccga cggcacggaa ttggtgcgcc aaggttgcgg
     4021 cgatacggcg gtgttcggtt tgtagggaaa ccgatgccgt ctgaagcatc ggctgttcag
     4081 acggcattgc gcgccttgcc ggcggcagtc cgaaatgccg gcgcgtatcg cgctcggtcg
     4141 gaatatccgt ttgaaacggc attttgatgc attactgcac cgcaatcgga attctcggtt
     4201 cgtagagcag gtcgtaggtc ggcttgttga gcaggtcttg gagcgtgaaa ccgtccagat
     4261 acgtgaaaaa cgacttcatc gcgccgccga gtatgcccgt cagccggcag gacggtgtaa
     4321 tcaggcattc gttgttctcg cccatgcact cgaccagctg catcggttcg aggtggcgga
     4381 caaccgagcc gatgttgatg cggtcgggcg gtgcggcaag ccgcagaccg ccgccttttc
     4441 cgcgcacact gtggaggaag ccgcctttga ccagcgcggt aacgaccttc atcagatggc
     4501 ttttggaaat gccgtaggtt acggcgatgg tactgatgtt gaccagcgca tcgtcgttga
     4561 tggcagtgta gataaggacg cgcagcccgt agtccgtatg ttgtgtcaaa tacatgattt
     4621 tctcggtatg gattgttatt cttatcggta cggtttaagg ttcacggaca ataccttaat
     4681 ggttgaaacc ctgtccgtcg gggcggtaga atgcagcctg tctgcggcgg tatgccgtct
     4741 gaaacatccg cgctaccgtt tgagaatttg ttattgtaac tcaaaatcat gaaaccgttg
     4801 aaacgacatc ccgcccttat cgggctttcg cgtgaccacc accattcgct ttccctgtgc
     4861 gtgcgtctgt tgcggacgcc ggaagaaagg catcgggacg aactcgaacc gcatttttcc
     4921 gaattggaaa cccattttcg cgaagaagaa accaagtttg ccccaatttg gcagaatgtc
     4981 gcccccgaat tgaaacaacg tttcgagaaa gaccacgccc gactgcggca gatgatggca
     5041 agccccgaat acggtaacgc ggcgtggaat accgcttttg ccacaaccct gcgcgaccac
     5101 gcgcgctttg aagaacgcga gctgtttccc gccgccgaac cgtttttgcc ggcatgattc
     5161 cgttttgcgg taaatatatt aatgataaac aaggaacaca catgaaattt accaagcacc
     5221 ccgtctgggc aatggcgttc cgcccatttt attcgctggc ggctctgtac ggcgcattgt
     5281 ccgtattgct gtggggtttc ggctacacgg gaacgcacga gctgtccggt ttctattggc
     5341 acgcgcatga gatgatttgg ggttatgccg gactggtcgt catcgccttc ctgctgaccg
     5401 ccgtcgccac ttggacgggg cagccgccca cgcggggcgg cgttctggtc ggcttgacta
     5461 tcttttggct ggctgcgcgg attgccgcct ttatcccggg ttggggtgcg tcggcaagcg
     5521 gcatactcgg tacgctgttt ttctggtacg gcgcggtgtg catggctttg cccgttatcc
     5581 gttcgcagaa tcaacgcaac tatgttgccg tgttcgcgct gttcgtcttg ggcggcacgc
     5641 atgcggcgtt ccacgtccag ctgcacaacg gcaacctagg cggactcttg agcggattgc
     5701 agtcgggctt ggtgatggtg tcgggtttta tcggtctgat tggtacgcgg attatttcgt
     5761 tttttacgtc caaacgcttg aatgtgccgc agattcccag tccgaaatgg gtggcgcagg
     5821 cttcgctgtg gctgcccatg ctgactgcca tgctgatggc gcacggtgtg ttggcttggc
     5881 tgtctgccgt ttttgccttt gcggcaggtg tgatttttac cgtgcaggtg taccgctggt
     5941 ggtataaacc cgtgttgaaa gagccgatgc tgtggattct gtttgccggc tatctgttta
     6001 ccggattggg gctgattgcg gtcggcgcgt cttatttcaa acccgctttc ctcaatctgg
     6061 gtgtgcatct gatcggggtc ggcggtatcg gcgtgctgac tttgggcatg atggcgcgta
     6121 ccgcgcttgg tcatacgggc aatccgattt atccgccgcc caaagccgtt cccgttgcgt
     6181 tttggctgat gatggcggca accgccgtcc gtatggttgc cgtattttct tccggcactg
     6241 cctacacgca cagcatccgc acctcttcgg ttttgtttgc actcgcgctt ttggtgtatg
     6301 cgtggaagta tattccttgg ctgattcgtc cgcgttcgga cggcaggccc ggttgagaca
     6361 aaccgccgca gatttcgggt ctgggcggtt tgcttttcag acggcagggc ggtcagttgc
     6421 cgtccagcca gcggtcgcgt gtggttttgg cttcttcaaa atagcggtac agggcttcgc
     6481 ggtcgtcggt ggtcaggatg tttgccaaaa cgtccaactg tttgcccaag ccttgaacca
     6541 gttgcagcag gctgtctttg ttggcaaggc agatgtccgc ccacacggcg ggatgaccgg
     6601 aggcgatgcg ggtgaagtcc cgaaagcccg tggcggcgaa tttcagatat tcctgtccgt
     6661 cggggtggtc gagaatctgg tggacatagg cgaaggcggt caggtggggc atatgggaga
     6721 cggcggcgaa aaccgcgtcg tggcgttgcg cgtccatcgt ataaatttcc gcaccgaccg
     6781 cgtgccacag gttttctacc aaggcaatgc cgtctgaatg ttcgccgccg tgtggcgtga
     6841 tgatgagttt tctgtggcgg aacagcccga actgcgcggc ttgcgcaccg cttctgtccg
     6901 aaccggcaat tgggtgggcg gcgatgcagt ggtgcaggcg gtcgggcaga cagcggcgga
     6961 aggcttcgat gaccgaagat ttggtgctgc cgacatcgga aatccaagtg tgttccggca
     7021 aaacggggcg cagcgcggtc aaaatggcgg gaacggtggc gacgggcgtg gcaatcagta
     7081 ccaagtccgc accgccgatg ctgtccgcgt cgatggcaac ggaagcctgg tcaatcacgc
     7141 cgcgttccaa tgcacgttcg aggttgtcgc ggtcggtgtc gataccggta acggtgcgga
     7201 cgagtccctg ccttttgagg tcgagaacga acgaaccgcc gatcagccct acaccgatga
     7261 gggcaatatg gttcaaaatg ggcatttgtg taaacggttt tcgcaaagta ccgtcatggt
     7321 agcctatcgg cggaatatgc cgcaaggtcg gcaggaaaaa ggagaagaaa tggacaaaat
     7381 cagagttgcc gccgtgcaga tggtgtcggg cgtgtcgccg gaaaccaacg tcgccgccat
     7441 gaaacgcctg gtcgcacggg cggcggagca gggtgcggat tgggtgctgc tgcccgaata
     7501 ttgggtgctg atgggcgcaa acgataccga caaactcgcg cttgccgagc ctttgggcgg
     7561 cggacgcttt cagacggcat tgagcgaaac ggcgaaagaa tgcggcgtgg tgctgttcgg
     7621 cgggactgtg ccgctgcaaa gctgcgaggc gggtaaagtg atgaatacgc tgttggtgta
     7681 cggacgggac ggcgtaagga cggggctgta ccacaaaatg cacctcttcg gtttttccgg
     7741 tttgggcgaa cgctatgccg aagccgatac catccgcgcg ggcggggatg tgccgcactt
     7801 gtcggcagaa ggcgtgccgg tggcggcggg catttgttac gatgtccgct ttcccgaatt
     7861 tttccgacgc cagttgccgt ttgacgtatt gatgctgccc gctgcgttta cgcacacgac
     7921 gggcaaggcg cattgggagc tgctgctgcg cgcgcgtgcc gtcgaaaacc aatgttacgt
     7981 cgtggcggcg gcacagggcg gtttgcacga aaacggacgg cgcacgttcg gacacagcat
     8041 gattgtcgat ccgtggggcg acgtgttgga cgtattgccc gagggcgaag gcgttgttac
     8101 ggcagacatc gatgccaacc gcctgaacag cgtccgcaac cgcctgcccg ccttgaaata
     8161 ccgggttttg gatgccgtct gaaggttcag acggcatcgg tgccggggaa tcagaag
//
LOCUS       DAWIVB010000086         8146 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWIVB010000086 DAWIVB010000000
VERSION     DAWIVB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.086"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(276..1004)
                     /gene="rmpM"
                     /locus_tag="WIX60_001286"
     CDS             complement(276..1004)
                     /gene="rmpM"
                     /locus_tag="WIX60_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ5305552.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1368..2318
                     /locus_tag="WIX60_001287"
     CDS             1368..2318
                     /locus_tag="WIX60_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ5305553.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2431..3165)
                     /gene="fnr"
                     /locus_tag="WIX60_001288"
     CDS             complement(2431..3165)
                     /gene="fnr"
                     /locus_tag="WIX60_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ5305554.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3370..4791
                     /gene="hemN"
                     /locus_tag="WIX60_001289"
     CDS             3370..4791
                     /gene="hemN"
                     /locus_tag="WIX60_001289"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ5305555.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5144..6718)
                     /locus_tag="WIX60_001290"
     CDS             complement(5144..6718)
                     /locus_tag="WIX60_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ5305556.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            7043..8143
                     /locus_tag="WIX60_001291"
     CDS             7043..8143
                     /locus_tag="WIX60_001291"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ5305557.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTADLNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
BASE COUNT         2057 a         2279 c         2002 g         1808 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tctgaatctg aatctgaatc tgaatctgaa
       61 tctgaatctg aatctgaatc tgaatctgaa tccgaatccg aacctgcacc ccgattctga
      121 cgatagccgt ttcagaaaac cacccaaaaa aacaaacccc gcaaaagcgg gggtttgttt
      181 cacatcatcg atgccgaccg acgtttcacg aatattgcaa cagtacctgg tacaaaatcc
      241 actaacctca tgcatcggca agatattgcc tagccttagt gttggtgatg attgtgtgcc
      301 ggcacaacct gacgggttac gatgctgcgg attttcacat ccacacggcg gtcaggttcg
      361 atacatgcaa tcagagcctc acgttttttg gctttagaga ctttcgcacc cagtttggca
      421 acttcggctt cacaaacttg agtcatttgc gcttgagatt cgcccaagcc gacagcagaa
      481 attctagaaa caggtacgcc gttgctgacc aggttgtttg ccactacgta tgcgcggcgt
      541 tcggacaggg cctgattgta tttgtcagaa cccataaagt cggtatggcc ttcgacgcgg
      601 acagattgga cattggttcg actcaggcgt tgcgccaata ctttcaggtt gtcttgagct
      661 tcggcgcgca atgaatcctt atcgaaaccg aacagggttt tggcagacag ggaaatggtt
      721 tcatcaacat attgcggagc ctgctccaca acgacgacag gcgcgggtgc gggttcgggt
      781 tctggctcgg gttcgggggc agcaaccgca tcgccgcatt ctacgcgacc ttggcttgct
      841 ttatcaaagt aggcgttttt ccagcattcg ccatagttgt tgcgtacgat ttcgttcgac
      901 tggccgctta cggtgtaacc ctgaacggac gcctcgcccg caacagcagt gccggaagcg
      961 agcaatgcaa cgaataatgc gcttaatttc agctgtttgg tcattttatt ccctcattaa
     1021 atttgtacag cagatacaga agcaactgct gaattgccaa catacaggcc gctgtagaaa
     1081 catggcggca aatcggatat ttcagtctcc actataaaga agcagggggc ggactgtcaa
     1141 tactcaacgc ctgaaaacaa ccgtaaaaca gcctgtttcc tgatagtcta tcatgccgaa
     1201 aaatcaattc cgtgtcatta ctttaggggg attttttcca tatcgcacga ttgccgttgc
     1261 acaaaaacaa caaacacaac ggcaaagccc cataccgtac cggcaagaaa atatgataaa
     1321 ttataaacaa tgttcagcca cccgacacag acccacaccg acccgccatg aaattacaac
     1381 aattgaaata cgccttagaa gtttaccagc acaacctgaa cgtttccgaa gcggccgaag
     1441 ccttgtttac ttcgcaaccc ggcatctcca aacaaatcaa attgctggaa gaagaaatcg
     1501 gcattcagat ttttatccgc agcggcaagc gcgtggtttc ggtctcgcag ccgggcaagg
     1561 tggttttgga tattgcggaa cgtattttgc gcgatgttca gaacattaaa aatatcggca
     1621 gcgagtttac cggacaggac agcggttcgc tgacggttgc cacgacgcat actcaggcac
     1681 gctatgccct acccttgatt gttgccgatt ttgtgaaacg ctatccgaaa gtcaacctga
     1741 ccatcaaaca ggggagccct gccgccatcg cacgtatggt tacctcaggc gaggcggact
     1801 tggcgattgt tacggaacgg atagacgacc atcccgaact gggaaaactt ccctgctatg
     1861 actggactca tgcggttatc gtaccgaacg accacccctt gctcgaatgc agaaaccccc
     1921 tccgtattga agatttggcg aggtttccgc tgattactta tgaatttgca ttcaatgcgg
     1981 gcagcagcat cgcgcgggca ttttccaaag cccgtttgga acaacccgat gtcgcattgg
     2041 ctgcggcaga tacggacgta ttgaagactt atgtgcgctt gggtttgggc gtgggactga
     2101 tggcgaaaat ggcgtacaac ccggatacgg acggcgattt gcagcttgtg gatgcggcac
     2161 acctgttcga gccgtcgccg acgtggattg ctttgcgcag cgatacttat ttgcgcggat
     2221 atgcctacga ctttatccaa gcgttcgcgc cgcacctgac acgcgagaag gtggatagga
     2281 ttctgtacac gcccatcagc gaggattttt cgatttaggc ggctgccggt tttcagacgg
     2341 cactttgcgg cagatacaac aaacaggaca gatgttttcg tctgccctgt gtttattgag
     2401 aatgctgtct gaaatgttcg tacgggttaa tcaaatggcg tgcgagcagc cggacaccat
     2461 ttttttcaac acctgcagat tgaggatttt gatgtgctta tgctcgacgg aaatcaatcc
     2521 ttcctgatga aatttagata atgtgcggct gacggtttca agtttcagcc cgagataact
     2581 gccgatttct tcgcgggaca ttcttaagat gaagtcgttg gcagcaaaac ctcgggaata
     2641 aaggcgttgg gaaaggttca gcaggaaggc ggcaatccgc tcttcggcgc gcatattgcc
     2701 caacagcagc ataacacctt ggtcgcgcac gatttcacgg ctcatcatgc ggaagaagtg
     2761 cgtacgcagg ctggggatgt tttgccccag ttcttcgatg tgggtaaacg gcagttcgca
     2821 cacttcgctg tcttccaagg cgaccgcgtc gcaactgtgc acatgggaac agatgccgtc
     2881 catgccgatg agttcgcccg acataaagaa acccgttacc tgatcgcggc cgtcctgact
     2941 ggcgacggtt gttttgaaga agcccgaacg gatggcaaag agcgaggtaa aggcttcgcc
     3001 gacacagaac aggtattcgc cctttttcag gcggcggctt tgacggatga cggcatcgag
     3061 ttggctgagc tcgttgggca gcagcccgac aggcaggcag agttcccgca aagaacagga
     3121 agaacacagc gttttcatct gatgtgtagt attatgcgaa gccataccgt acctttttgt
     3181 gcgctttgcc ccatcatgat tatagtggat taaatttaaa ccagtacggc gttgcctcgc
     3241 cttgtcctta tttaaattta atccactata tgtgcttatt gacacatatc aagacaggtt
     3301 tatcatactg tggcattcta ccaaactatc caaaatttga ctaacatcat aaaaccgcac
     3361 acacacacca tgaaaatcat tcagatacag aacaatcaca atgtaaacga tgaccgcccc
     3421 gagtttgacc gcgcgctgat tgccagcctg cccgccagcg gcccgcgcta cacttcctac
     3481 cctaccgccg accgtttcca tgacggtttc cgcgaaggcg aatatatcaa agctttacat
     3541 ttgcgcggta tgggcgcgtt aaacaaaccg ctttcccttt acattcacat tccgttctgc
     3601 aacaccatct gctactactg cggctgcaac aaaatcatca ccaaagacaa aagccgcgcc
     3661 gatgcctaca tcgaatatct tgaaaaagaa atggaactgc tcgctccaca tctgaacgga
     3721 cggcaccagc ttgcccaact gcacttcggc ggcggcacgc cgaccttttt gagcgacgaa
     3781 cagatcgaac gtgtcttccg catgatacgc aaacatttcg agttaatccc caccggcgaa
     3841 tactccatcg aaatcgaccc gcgcaaagtc agccgcgaca ccgtcctcat gctcggcaga
     3901 ctcggcttca accgcatgag catcggcatt caggatttcg accccaaagt gcaggcggcg
     3961 gtcaaccgca tccaaagtta cgaagaaacc aaagaagtca tcgatgcggc gcgcgaagcg
     4021 gggttcaaat ccgtcagcgt cgatttgatt tacggcctgc cgcaccagac ttcggaaagc
     4081 atcaaaacca ccatcgatac cgttttgtcg ctcgatcccg accgcctcgc cctttatcac
     4141 tacgcccacc tgccgcacgt gttcaaaccg caacgccgca tcgataccgc cgccgttccc
     4201 gacagcgaag agaagctcga tatgctgcaa tactgcgtcc aaaccctaac cgaacgcggc
     4261 tacgtcttca tcggcatgga tcatttcgcc aaacctgacg acgaactctc catcgccctc
     4321 aaagaaggct tcctccagcg caacttccaa ggctattcga cctacgcgga ttgcgatttg
     4381 gtcgccatcg gcgtgtcgtc catcggcaaa atcggcagca cctattccca aaacgaacgc
     4441 gacatcgatg cctactatgc cgccatcgac gaaggcagac tgcccatcat gcgcggctac
     4501 cagctcaatc aggacgacat cctgcgccgc aacatcattc aggatttgat gtgccgtttc
     4561 gcgctcgact atcggattta cgaaagtatg ttcggcatcc cgttcgaccg ctacttcaaa
     4621 gacgaactgg cggatttgga aaaactcgcc ggtttgggat tggtgcgcct gaacagccac
     4681 gggctgaccg ttaccccgaa aggacgcttc ctcatccgca acatcgccat ggtcttcgat
     4741 taccacctgc gccataaaga aaccaaggcg aaatattcgc aaacagtgtg attgtggcta
     4801 acgtacaaat gccgtctgaa aggctttttc agacggcatt ttgctgccgg caggataagt
     4861 gttttcaaga acaggcggcg gcatatcata acgttccgca cctttgtgtc cgaccgttcc
     4921 gaaaccaaga tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttgccgtact
     4981 agctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcac tataccaaac
     5041 ggcatatccc gacagaacag attgtgcata aggcacaagc ccgcacattc catcaacaaa
     5101 atgccgtctg aacacgggtt cagacggcat cagtatttta caatcagaat actgcctgta
     5161 aaaccaagta gcagacaacc gacaatacgg cggcggcagg cagggtaatg acccacgcca
     5221 aaccgatggg cttcatcagt ttccagttgg cattgcggtt gaccagaccg ataccgagta
     5281 ccgcgccgac caagatatgc gtactggaca cgggcagccc catcagcgac gcgcccatca
     5341 cgacggaggc ggcggacagt tcggcggtaa aacccgaagc aggatgcatt tccgccaaac
     5401 tcgtaccgac ggttttaatc acctctttac cgacaaacca caaaccgaca atcagcgcga
     5461 tgccgaaagt cagcatcgca atcgggggga cgacattttg cgcggcaacg ctgttggtac
     5521 gcaaaacatc cataatcgcg gcaaacggac cgatggcgtt ggcgatatcg ttcgcaccgt
     5581 ggctgaatgc gaagccgcag gcggtaaaga cctgcatcca tgaaaacatc tgaaaggtcg
     5641 atttgcccaa gtctttacgc ttgaggcttt tggcaaaaac aaacgtcccc atccacaccg
     5701 ccgcgcctat cataaagatg gtcaggaagc tgttgacgtt gctcatcccc aaatgcaggt
     5761 ttttcaagcc cttgaaaatc agcatagcgg aaatcatcat cgcgccgaac gaagcgataa
     5821 agggaatcca agaatgcagt gccttgtagg aatcgacatt gtttttacgg ttgtcgaacg
     5881 cataaagacc gcggtaatac tccgattgca gctcttgcgg atcgaattcg ggttcgtcgt
     5941 aaatttgcgc gtcgtgcgcc attttggtgg cgtactcgac tttttcggct tcggacaaac
     6001 cctcgaaaaa caggcggtgc cgttctttat aggccttttt ttcctgcttg atgcccttga
     6061 gcgtgccttc cgcccaagcg ttgtaatcta agacgttttt cttgacgcgc gaaaacagaa
     6121 aataggacac cgcgccgccc aacacgggcg acaataccca agaaacacca ataccgccca
     6181 gcttgcccca acgtatcaaa tcgcccgatg cggcatcgtt cattaccgcc atacataccg
     6241 cgctgccgac aatgccgccg ataatggaat gggtggtaga taccggaagc ccttttttcg
     6301 aggcaaacaa cagccacaac gccgccgcca aaagcgcgga catcataata aacacaaact
     6361 gtatgggttc gaaatcaaca cccttcaaat cgacgatgcc tttgcgtatg gtattggtta
     6421 cctcgccgcc cgcgatgacc gcgccgctga cctcaaatac cgccgcaatc agcaaagcct
     6481 gcgggatggt cagcgtaccc gcaccgacgc tggtgccgaa agaattggca acatcgttgc
     6541 cgccgatgtt gaacgccata aacacgccga acatcgtggc aataatgaat aaggcggcgt
     6601 aattgtaatg ggtataaccc aagccccaat aaataaaata gccgactgta cctaccagca
     6661 tggcggcaaa gacggcgttg atggttttaa catttgcgct catttggatt tgagccatgg
     6721 caatcttcct ttaatgtggt ggaaatcatg agtgttgccg acactgtact gctgcctccg
     6781 gtcgaactgc cgtttcggga tatggcagac gcatccttcc ttctgaaccg catccgaata
     6841 cccccaaacc tgaccgaatg caaaactgct gccggaaacg gtttgtatga cacttctact
     6901 actggatgtt gcgggcgcat tataatattt ttccatccgt cttgaaacat ttatttacac
     6961 tttatttaca ctgcggcggc aaatcggtat acgagcgtca atacacgtta aaatggcgtt
     7021 ttgcaccagt ttgggagtga tgatggaaac acagctttac atcggcatca tgtcgggaac
     7081 cagcatggac ggggcggatg ccgtactgat acggatggac ggcggcaaat ggctgggcgc
     7141 ggaagggcac gcctttaccc cctaccccgg caggttacgc cgccaattgc tggatttgca
     7201 ggacacaggc gcagacgaac tgcaccgcag caggattttg tcgcaagaac tcagccgcct
     7261 atatgcgcaa accgccgccg aactgctgtg cagtcaaaac ctcgcaccgt ccgacattac
     7321 cgccctcggc tgccacgggc aaaccgtccg acacgcgccg gaacacggtt acagcataca
     7381 gcttgccgat ttgccgctgc tggcggaacg gacgcggatt tttaccgtcg gcgacttccg
     7441 cagccgcgac cttgcggccg gcggacaagg cgcgccactc gtccccgcct ttcacgaagc
     7501 cctgttccgc gacaacaggg aaacacgcgc ggtactgaac atcggcggga ttgccaacat
     7561 cagcgtactc ccccccgacg cacccgcctt cggcttcgac acagggccgg gcaatatgct
     7621 gatggacgcg tggacgcagg cacactggca gcttccttac gacaaaaacg gtgcaaaggc
     7681 ggcacaaggc aacatattgc cgcaactgct cgacaggctg ctcgcccacc cgtatttcgc
     7741 acaaccccac cctaaaagca cggggcgcga actgtttgcc ctaaattggc tcgaaaccta
     7801 ccttgacggc ggcgaaaacc gatacgacgt attgcggacg ctttcccgtt ttaccgcgca
     7861 aaccgtttgc gacgccgtct cacacgcagc ggcagatgcc cgtcaaatgt acatttgcgg
     7921 cggcggcatc cgcaatcctg ttttaatggc ggatttggca gaatgtttcg gcacacgcgt
     7981 ttccctgcac agcaccgccg acctgaacct cgatccgcaa tgggtggaag ccgccgcatt
     8041 tgcgtggttg gcggcgtgtt ggattaatcg cattcccggt agtccgcaca aagcaaccgg
     8101 cgcatccaaa ccgtgtattc tgggcgcggg atattattat tgagcg
//
LOCUS       DAWIVB010000087         8128 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWIVB010000087 DAWIVB010000000
VERSION     DAWIVB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.087"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..163
                     /locus_tag="WIX60_001292"
     CDS             <1..163
                     /locus_tag="WIX60_001292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305558.1"
                     /translation="APIAKAMMLNQGFCEAFIVVVLMGIAAIMRKRPYFFKPPHRFRV
                     CPSSRPDLQ"
     gene            201..413
                     /locus_tag="WIX60_001293"
     CDS             201..413
                     /locus_tag="WIX60_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ5305559.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            561..2144
                     /locus_tag="WIX60_001294"
     CDS             561..2144
                     /locus_tag="WIX60_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ5305560.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICWNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2226..3323
                     /locus_tag="WIX60_001295"
     CDS             2226..3323
                     /locus_tag="WIX60_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ5305561.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3437..4552
                     /locus_tag="WIX60_001296"
     CDS             3437..4552
                     /locus_tag="WIX60_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ5305562.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4677..5132
                     /locus_tag="WIX60_001297"
     CDS             4677..5132
                     /locus_tag="WIX60_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ5305563.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5203..5679
                     /locus_tag="WIX60_001298"
     CDS             5203..5679
                     /locus_tag="WIX60_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ5305564.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            5724..6017
                     /locus_tag="WIX60_001299"
     CDS             5724..6017
                     /locus_tag="WIX60_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEZ5305565.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            6014..6286
                     /locus_tag="WIX60_001300"
     CDS             6014..6286
                     /locus_tag="WIX60_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEZ5305566.1"
                     /translation="MKMQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVV
                     FNLFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            6469..7386
                     /locus_tag="WIX60_001301"
     CDS             6469..7386
                     /locus_tag="WIX60_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ5305567.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTDLCT
                     PEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPAGRDFDGEKLTNFFNDLPNMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            7451..8008
                     /locus_tag="WIX60_001302"
     CDS             7451..8008
                     /locus_tag="WIX60_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5305568.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TAGTIAMARTTAPDSATSQFFINLADNASLDYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         2162 a         1946 c         2103 g         1917 t
ORIGIN      
        1 tgcgccgatt gccaaggcaa tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 tgtcctgatg ggaattgccg ccattatgcg caagcgtccg tattttttca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagaatgcag
      181 taccaacgaa aggaaaaatc atggaaaccc ttatcctcga catcggcggc atgagctgcg
      241 gcggctgcgt caaaagcgtt acccggatat tggaaggcgt aaaaggcgtg gcaagcgtcg
      301 aagtcagcct tgaaaacaaa agtgcgaccg tcggatacga ccccgcgcaa accgatgcag
      361 gggcgttgat tgaagccgtt gaagacggcg gctatgatgc cgcgttgaaa taaagcggca
      421 aaaatgccgt ctgaagcctt cgcgcctccc agatgccgtc tgaagtacgc tccgcgcctt
      481 cagatggcat ttgatttagt tgaacgggta ggatgaattg gttaaacttg cgggcaatag
      541 tgccttcaga caaacaaaat atgccgaaca agaagaaaaa agccgccttt gcgcgtgctg
      601 caacggagaa cggtgtgccg gacgtgtcca acatcaaaaa ttcatatgat gatttgatgt
      661 acgaatccgg cgcattctct caaaccgcca tcaataattt ggaggcgcgc gcccgcctga
      721 tgggtttgca gcccgcgccc gccgccaatg ccaaggtctt ggagctgggc tgttcgatgg
      781 gcggaaacat catcacgcag gcactttatt acccggatgc ggaatttgtc ggtatcgacc
      841 tgtccggcag gcaggttgca cagggcaacg ccatcattga aaaaatgggc ttgaaaaatg
      901 tgcgtctgga agaaaaagat attttgacca tcgatgagtc attcgggaag tttgactata
      961 tcatcgtcca cggcatttgg tcgtgggtgc ctgacgcagt taaagacaaa attttttcga
     1021 tttgctggaa caacctgacc aaacacggca ttgcctatat ttcatacaat gtttaccccg
     1081 gctggaaacg gcaggagcag ttgcgcgaaa ttatgtactt tgccggcagg gatgtgcttg
     1141 aagaaccctt ggaagcgcgg acgcggaaag gcttggacgc gctcaaggcg ctggcggaaa
     1201 ttttggaaaa cgacaagggc ttggacggcg gcggcaaact tccggcgatt caaaaaatat
     1261 tgaatcataa tttttattac atcgcacacg aatatatgga ggcgtttaac gacccgattt
     1321 acgtcaacgg cttcatcgaa tgggctaacc gccacaggct cgcatatatc ggggatacca
     1381 atttgcacgt gtcttttgtt tcttggatgg cggagcatac gcgggagcgg attctggcat
     1441 tggcagggga cgattatatt gccaaagaat tttacagtga tattttatcc gaccgccaat
     1501 tccgccgttc gcttttatgc cgtgaggaag tcggggatac tgtcaggcgt gatgagtcgg
     1561 ttgccgtcga agtgatagaa agtttgaatt tccgcccggc aagaggggaa acaatcaact
     1621 ttgatgaaaa cgatatcctg ctttcgggca tacgcgatgt gatgaaaacc ggagaggcgt
     1681 ttaaaacgga agatgttgcg gaaaatctcg cccgccgatt tcccggtttg gaatttgacc
     1741 gcatgaaaat caattcccag cttttattgc aaaccattct cgggcgtttt tctgtttcat
     1801 cagacaatgc gggcaaacca ttttttgaag accataaaac ctatgtgccg gcgcgcttta
     1861 caaactatgc cgccgccttt gtggaacacg gcgcggaagc gtttgtccgg cctgccaacc
     1921 gttacaacga aagcaccccc tcattcggat acgggcattt gtacattatg cgccaattgt
     1981 cgcggccgac gagcaaacag gcattgattg aaacggttgc cgaaaacttg aacatcgtca
     2041 gcaccacgcc cgacggtttg acattccatc cgcctgccga agtgtatgtg gaagaaatat
     2101 tggcagactt ggcagacagg cattttctcg tttcggcgga ttgacgggca agtcccggcg
     2161 gcaatccgtc cgatatattt tcagacagga aagacacaca ctgcgaaaag cggtgcacga
     2221 cacatatgaa ccagacattt accttgcccg atacgcgccc gtatccccaa aatccgatta
     2281 aaaaccacct gctgctcaat gcctaccagt tggcgcataa ttcttcccag gcttcgcgca
     2341 aactctcgtc cggccaactt caaaccgaaa tcaggggaat gcttgagcaa aaccactata
     2401 tcaacctttc cctcgcgctg acgatgtcgc ccgatgccgg aacttatgcc gcgctgcttt
     2461 ccagtgtgaa cgcggtactc gattgcgaga aagaaggcga agtgcagtgg ttcgccctgc
     2521 cggtcgtgct ggtgtccggc tgcaaaaaag aacgtgcaat cgagatgaag ctgccgacgg
     2581 aggcattgtt tgcctgcctg caaaactatc cgcacctgcg cgcgttgacg caagagacgc
     2641 aatggctgcc ttatcttgtg cattcttccg atttgagcgc ggtcgcgccg gatgagtggt
     2701 ggcgtgccaa acaaaatacc gaagcggcgg cgcaacactt gcgccgtttc gccccgcgcc
     2761 ctttgctgtt gcccgaaggg cagtccgtcc acgtcgttta cgcgctgggt ttcggcagcg
     2821 gcaaagttca gacggcattg ggtcagaacc ttcttcaggc aggtctgccc ctgatgcagg
     2881 tatggcagga aaatcttgca tcggaaggcg ttacgctgtt tgccaatccg ctttccccgg
     2941 attctccggt acgcgcgctt tcagacggca gccacacgcg ccaacgtatg gcgatggatg
     3001 tgtttgcggc aaacgcgata cgcgccgtcc gtatgcagag tccgcgcgtc ggcgtggtcg
     3061 ctgcggcaaa ggcgggcgga cagattttat tcgggtttaa tgcgaccgac ggcgcgtttg
     3121 aagtcgtgcc gcaggtgttt tcgtggcagc tttcctttac cgacaacatc gccgtcatcc
     3181 agcaaaattt cctcgacctg atggcggagt gccgcgtgga acacgtttac ctgttgcaca
     3241 atcccttggg cgaacaggaa agcatcccga gctatgcgga agcattgaaa cgggaagggc
     3301 acaatccgtt tttcagcgca tagtgatttg tagtagtgat aggctttctc atttatataa
     3361 taaatcaaaa taaagagcac aacacttttt cattctgtgt tgtgccttga gtgaaacgaa
     3421 aggatgaatt ataaatatga agattgtaat tgcaccggat tcgtttaagg aaagcttgac
     3481 agctcaacag gtagctgaag caataaaaag aggcttccaa caatcgatag cagatgtgga
     3541 atgtctcctc tgtcctgtcg gtgatggggg agaaggcact gtagatgcta tccgacattc
     3601 tcttgaccta gaagaaaaat gtctccaagt gacaggacct tttggacaaa aagaagtcat
     3661 gcgctatttt caaaaagaac aactagccct attcgaagtt gctgatttgg ttggtcttgg
     3721 aaaaatcccg ctagagaaac gaaatccatt acaaatccaa actcgtggta ttggagaatt
     3781 gattcgccac ctcattagtc aagaaattaa agaaatctat attggcgttg gcggtacggc
     3841 cagtaatgat ggaggtattg ggattgctgc tggtttaggt tatcaatttt atgatgagga
     3901 tggaaatgcc ttacccgttt gcggtcaatc cttactaaac ctagcttctg tttcaacaga
     3961 aaatcgctat gaaattcctg aagatgttca cattcgtatt ttagcagatg ttgtgagtcc
     4021 cttatgtgga caccaaggtg cgacctatac gtttggcaag caaaaagggt tggattctac
     4081 tatgtttgag gccgtagatc aggcaataca agatttttat gaaaaagtct cccctgcaac
     4141 attaaaactt aaaggagcag gagctggtgg aggcatcgct ggtggtttgt gcgcctttgc
     4201 tcaggcaagt atcgtatctg gaattgacac ctgcctggac ttgattgact ttgataagaa
     4261 agtgtcagat gttgacttgg ttatcgttgg tgaaggaaga ctagatcgtc aaagtttagc
     4321 agggaaagcg cctattggtg tagcaaaaag aacccctgtc ggagttcctg ttgtcgctat
     4381 ttgtggcagt ctcgttgaag atttgccttc cctgccattt gaaaatatcc aagctgcctt
     4441 ttctattttg gagaaaagtg aacctttaga agatagtttg aaaaatgcca gcctctattt
     4501 ggagcatacg gcttctaata ttggacactt attaaatatg cctaagattt agccaaacca
     4561 tttcttccag atggatgttt tggctggttc tgccttatgc ggttacgatt aaccgaccga
     4621 tgccgtctga aagggtttcc gcttcagacg gcatcgcgcc ggaacaggga ggggatatga
     4681 aaaaaccgaa aatccttttt gtctgcctcg gcaacatctg ccgttcgccg atggcggaat
     4741 acattttgcg ccgccgcgcc gccgaagcgg gcattcccct tgaagcggac agcgcgggga
     4801 catcgggctg gcacgacggc gaagatatgc accgcgagac ggcaaagata ttgaaaaaac
     4861 acggtatcga tgcttcaggc tttaccagcc gcaaaatccg ccaaagcgat gcggcggcgt
     4921 ttgactgcat catcgcgatg gacggcaaga atttgtccga attggaaaga accttcggca
     4981 ggcggccgga aaaaatattc aagctgaccg acctgatacc cgaaagcggt tacgaccatg
     5041 tccccgatcc gtggtatacg ggcgattttg aagaaacctt caggcttgcg gatgcgggct
     5101 gtcgggcatt gttggaaaag atttccaaat aaagcaattg aatacaaata taaaaccgcc
     5161 tcctgccgta tcggttgttc agacggcata aacagagaat ttatgaaaac aaatttcaaa
     5221 cagaaaatta tcgaacaggc acgcagcgaa ggcttgcagg taaccgcttt gcgcgagcag
     5281 gtattagata ttgtcttgca gcaaagcggc gtgattaaag cctacaacgt cttgtcgcag
     5341 atgcagcagc aaagcgaggg cgtgcttgca ccgcctaccg cctaccgcgc ccttgatttt
     5401 tgggcggagc agggcgtttt gcacaaagtg gcggcggtca acggctatat tttgtgcagc
     5461 cacgcgcagc acgagtgcga cgaccattgc cacgaccacg aagaagccga agcgcaccac
     5521 agcgcgttta ttttggtctg caccgaatgt ggcacggcgg acgagcaaac cctgagccac
     5581 gagtgggcgg cactgcgcgc aggcgttgcc gaaagcggct ttgcgctgaa agaagaacac
     5641 gttgttttaa ccgggatttg taaaaaatgt cagcagtaat cggtggtttg cattgataat
     5701 aagcctttcg aaaggagcag ataatgaaac cgtctctttt gttgcaaagc agaaagaaag
     5761 aaatattggc agttttcggg aaatatccat tgatttgcaa tccaagggta ttcggttcgg
     5821 tttctcgcgg agatgatacg gaaaacagcg acattgattt gttggtggat gcaaaaacag
     5881 ggacaacatt attggatttg ggcgggctgc aagaggaatt gcagaaactc ttgggcataa
     5941 aggtcgattt gctgacaccc gatgacatct cggcccactt tagagataag gtgttgactg
     6001 aggctgtcgc attatgaaaa tgcagaaaga attgtcggtc tatttgaaac agatattgca
     6061 ggcagcgcaa tatatccggc tatataccga caaaatggat tatgggcaat tttctgccga
     6121 cacaaagact gtgcaggcag tcgtttttaa tttgttcctt atcggtgaaa acgcaaccca
     6181 tatccttaaa tcgtatccgg aatttgccga ggaaaccaaa tatttgaatt ggatagggat
     6241 gagggggtat tgcgcaatag gattgcccat ggctactttg aaatgaacct gtctgtcgtt
     6301 tgggagacgg ttttgaacgt cataccggcg atgtcagccg atatatcgaa tttgctggaa
     6361 caattgtcaa ttgatgaaga acatgataac agctgccatc acttttaaca ttatttaatc
     6421 atttaaaaca ccctatcttc aaattttaaa gtgggacatc acatagaaat gaaaaaaacc
     6481 aaagtccacc tgatttcagg ttttctggga acaggcaaaa ccaccgcgct caaaagcctg
     6541 atggcgcaaa aagacccgaa cgaaaaatgg gtcatcatcg tcaacgagtt cggcgaaatc
     6601 ggcattgacg gcgcggtatt gagcgacaac ggcatccctg tggcagaaat cgccggcggc
     6661 tgtttgtgtt gcaccgccgg cccgcaaatg ggcgtaaccg tgcagaaaat gctgcgcgac
     6721 gccaagcccg accgcctgat gattgaagca agcggactgg cgcacgccgc cagtgtcatt
     6781 gatgaactga aaaccaaacc gctggacagc cttttggaaa tcggcgcagt ctttaccgtc
     6841 gtcgatccgc gccagttcat caaccccgat tacgcgcagc aggcattgta taaagaccaa
     6901 atcggcatct gcgacgtatt ggttgccagc aaaaccgatt tatgcacccc cgaacagctt
     6961 gccgaatttc acgacaaagc cgcaaaactg ttcccgccca aggctaaagt ggtcgaagtt
     7021 caaaacgcac aactcgatat ccaatggctt gacatccccg tcatcgaaaa atcacgctac
     7081 cgcctcaaag ccctgccgga caacacgatg ggcttccagt cgcaaggttt cacattcccc
     7141 gccggacgcg atttcgacgg tgaaaaattg accaacttct tcaatgattt gcccaatatg
     7201 accgaaggac tcgtccgtgc caaaggcgtg ttccaagtat tgggaacgtg ggtgtggctc
     7261 aactgggtgg acgggcaatg gggtgcgaac caagtgtctt ggcggcgcga ctcgcgtttt
     7321 gaattgattg ccaaatcgtt tgatgcggat ttaatcgaac aaaaactcaa agacgcattg
     7381 gaatagcgtt aacgagcctt cagacggcat aggaaatgaa aatgctgtct gaaggtctga
     7441 ggaggacggg atgaaaccca aattcaaaac cgttttaacc gcgctgcttt tggcggtttc
     7501 cctgccgtct atggcggcaa cccatgtttt gatggaaacc gatatgggca atatccgttt
     7561 ggttttggac gaatccaaag cccccaaaac cgttgctaat ttcgtgcgct atgcccgaaa
     7621 aggcttttac gacgacaccg tttttcaccg cgttatcgac ggttttgtta tccagggcgg
     7681 tggattgacc gaggacttgg cacaaaaggc aagcgataag gccgttgcca acgaatccgg
     7741 caacggcttg aaaaacaccg ccggcaccat cgccatggcg cggacgacag cccccgattc
     7801 cgccaccagc caattcttta tcaatctggc ggacaacgct tcgctcgact acaaaaacgg
     7861 acaatacggc tataccgttt tcggcagggt cgaaagcggc atgaacaccg tttccaaaat
     7921 cgcccgcgtc aaaaccgcca cgcgcggctt ttatcaaaac gtacccgtac agcccgtcaa
     7981 aatccgtcgc gttgttgtcg ggcagtaaca cgcagacaga cgttcagacg gcgtcgcccg
     8041 tttcccaaaa aacgccgttt aaagtaaaaa aatattttaa aacagacagt tgatattgac
     8101 aaattcaaac cgaggatttt aaaatgct
//
LOCUS       DAWIVB010000088         8042 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWIVB010000088 DAWIVB010000000
VERSION     DAWIVB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.088"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(68..814)
                     /locus_tag="WIX60_001303"
     CDS             complement(68..814)
                     /locus_tag="WIX60_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ5305569.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WIX60_001304"
     CDS             complement(993..1664)
                     /locus_tag="WIX60_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ5305570.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WIX60_001305"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WIX60_001305"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ5305571.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WIX60_001306"
     CDS             complement(2481..2963)
                     /locus_tag="WIX60_001306"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ5305572.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WIX60_001307"
     CDS             complement(2995..3684)
                     /locus_tag="WIX60_001307"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ5305573.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPIADTLKCADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WIX60_001308"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WIX60_001308"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ5305574.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="WIX60_001309"
     CDS             complement(4571..5881)
                     /locus_tag="WIX60_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ5305575.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WIX60_001310"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WIX60_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ5305576.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WIX60_001311"
     CDS             complement(6056..6355)
                     /locus_tag="WIX60_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305577.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="WIX60_001312"
     CDS             6706..7902
                     /locus_tag="WIX60_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ5305578.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1900 a         2405 c         1965 g         1772 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgttgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaaga aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccataaaccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg ctcggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtatcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag atgaattttt cggacgcgct ggcgacgatt ttttcgttga
     2101 ggtgcgcgcc gccaccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 cgatatagac cgccaacccc gatacttcgt tcaaagaaac gacgggaata tcgtactggg
     2221 caagcagttc gccggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atatattcat
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatgcgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatcc gcccgagata gccgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat gtgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cacgtcgccc tgtatcagta gcggacgcac acccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3241 gtccgcgcac ttgagcgtat cggcaatggg aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcaac gcgcggcatc
     3361 gtgtaccaga atattgtcgg tttccgccgc caaaccggtt tccaacagtt ttgccacacc
     3421 gttgcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctgtatc gtcaagccgt ggacgcgtgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaagcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacagag caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcacgca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacggacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcctgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc cattaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaacaaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 ggcgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac caacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aaggttttat ccgcacacga
     7921 actgcctccg gaaatggagg cagttttttt atccggcttt ccatgcttaa acagcactgc
     7981 ctcttttcag acattgacgg ttgcagccgc ttacctgaac cttatagtgg attaaattta
     8041 aa
//
LOCUS       DAWIVB010000089         8016 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWIVB010000089 DAWIVB010000000
VERSION     DAWIVB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.089"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            52..1005
                     /locus_tag="WIX60_001313"
     CDS             52..1005
                     /locus_tag="WIX60_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ5305579.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WIX60_001314"
     CDS             complement(1116..1439)
                     /locus_tag="WIX60_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305580.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WIX60_001315"
     CDS             complement(1612..2973)
                     /locus_tag="WIX60_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ5305581.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGTGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAETIEVTTFRGGAKVHQNARGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAAHRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHLLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WIX60_001316"
     CDS             complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WIX60_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ5305582.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIAQLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVGRKEKILIVADYDADGATACAVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNDFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTTFEEAVRE
                     MVCEDDVSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5039..5755)
                     /locus_tag="WIX60_001317"
     CDS             complement(5039..5755)
                     /locus_tag="WIX60_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305583.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTLMLWGMNNPVSETITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEEHTDKSKLFANILYTPPFLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            complement(5802..6314)
                     /locus_tag="WIX60_001318"
     CDS             complement(5802..6314)
                     /locus_tag="WIX60_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ5305584.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            6461..7792
                     /gene="pmbA"
                     /locus_tag="WIX60_001319"
     CDS             6461..7792
                     /gene="pmbA"
                     /locus_tag="WIX60_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ5305585.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVKGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            7804..>8016
                     /locus_tag="WIX60_001320"
     CDS             7804..>8016
                     /locus_tag="WIX60_001320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305586.1"
                     /translation="MPSEDEGSTFCPVPARLPFYAAPPFADNIAHFFISTDFPLSVKI
                     LSHLSTTGYTQVQDKACGDWGFSAAGK"
BASE COUNT         1824 a         2341 c         2049 g         1802 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgccaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaaa acggctatat ccgcgccctg
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gttgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgc
      541 ccagccgtcg aagccggcga gaaactgggc ttcctgcccg gagacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgta
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcaggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gccgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgttgcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatgcata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccatg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggcctt gcgcctttgc ggttttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaacg cgcaaggcat tgagcagcag atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttt agacactcgc gagcgtgccc tgaaaacagc aatttcataa tttcgtcgaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggcggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc gccaatcata accagcctgt gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccatagg tattgtcttt
     2581 cataatcctg ccgcgtgcat tctgatgtac tttcgcaccg ccgcgaaacg tcgttacttc
     2641 gatagtctct gcaccgttca tcacatggac aatctgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgcgacat cgaaatcttt
     2761 gggttcgatg ccgagcagca ggtccctgac cgcgccgccg accacataag cctgaaaccc
     2821 cgtccctttc aggcggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcaggaa tgaccgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacaggca accgtgttca gacggcatca ggcgcggttt gcaagatgcc gtaaccataa
     3061 acaaaaggcg tagaaactac gccgtccgaa tcacgccgcc ctcgcggcgg caacgcgtca
     3121 ttataacaga ttgccctcca aaaaaatcag accctgcttt tgtggcagag tctgaaattt
     3181 gatgcgcctt ctccgattgg gaacattccc gtcgtacaaa caggcaaatg aaggaacaga
     3241 aatcacattc aaacagtgtt ccgccgcctc tacgcggctt cccagtaatc gatatacagc
     3301 tgcaattcga gattgttgcg ccattcgttg gcaacggggc ggtaaaccgt gcggatgtat
     3361 tcgggaatgt cttcgctgca acgccaaaac atcgcttcaa attcgcagcc gtctttttgc
     3421 agccagactt ttttgtgttt gccctccgcg cccaaaggtt gctggcggac gacgtggaac
     3481 tcgtcggtaa agctcggcgg cgcgaagccc tgcccccaaa cgtgacgggc aaggttttgc
     3541 gcctgttcca acgtgatgtc gcaggcgggc aggctgccgt cggtgatgaa ggtttgcgac
     3601 acatcgtctt cgcacaccat ttcgcgcacg gcttcttcaa aggtcgtctg aaacgcggga
     3661 atgttgtgtt caagtatgct caaacccgcc gccatcgcgt gtccgccgaa tttcaaaatc
     3721 aaatcgggat ggcgtttgga caccaagtcc aaagcatcgc gcaggtgcaa attgggaatg
     3781 gaacgtcccg aaccgcgtac ttcgccgttg tcggcaggcg caaacacgat ggtcggacga
     3841 taaaaacggt ctttgaggcg gctggcgaca atgccgacca caccttgatg gaagtcgtcg
     3901 cgatacgcca ccaaagtcat ctgacctgaa ggcagggttt cggggaaatc attcagtgcg
     3961 tcttgcagca tagactgctc gatttcgcgg cgctcgatat tgaggttgtt taactgagcc
     4021 gccagttcct gagcttcgga atcatctcgc gccaacaggc aggcgatgcc gaccgacata
     4081 tcgtccagcc gtccggcggc gttgatgcgc gggcccaacg caaaacccat atcaaacggc
     4141 tgcgccttgc gccaatcgcg ccgcgccact tcaaacaagg cgcggatacc ggggcgcatt
     4201 ttgccggagc gcatccgttt caaaccttgc gacacgagga tgcggttgtt gtggtcgaga
     4261 gggacgacat cggcaaccgt gccgagtgcg accaaatcca aaagttcgcc caaattcggc
     4321 tctttgatgc cgtctgaaaa ataattgcgg cggcgcaatt cggcacgcaa cgccatcaat
     4381 acataaaaaa tcacgcccac gcccgccaag cttttgcttg gaaaaccgca gcctttttgg
     4441 ttcggattga cgatgatgca gtcgggcacg gtctcggccg gcaagtggtg gtcagtaacg
     4501 atgacatcca aacccagagc ctgcgccctc gccacgcctg cgatgctggc gataccgtta
     4561 tccaccgtaa tcagcaaatc cacgccctgc gcggcagcga tttcggcaag ttcgggcgtt
     4621 aagccgtagc cgtgttcaaa gcggttgggc acaaggaaat ccactttcgc ccccatcgcc
     4681 gccaaaccgc tcataccgac ggcacacgcc gtcgcaccgt cggcatcata gtcggcaaca
     4741 atcaggattt tttccttgcg cccaaccgca tccgccaaac ggcgggcggc ggcttcgcaa
     4801 ttcgtcaacg attgataagg caggagggaa gcgagtttgt cgtccaattc ggcaggactt
     4861 tgcacaccgc gcgaagcaca aagctgggcg attaaaggat cggcaccggc ggtgagcaaa
     4921 tgattaaaaa cgtcggtatt gacggatcgg gtttggattt tgactgacat aacgtggggt
     4981 tttactgtgc gtgtgtgctt aaaatgccgt ctgaaagctt ttcagacggc attgctgttc
     5041 atttgcgggc ggcatccacg accgcaccca gagccgccgc aggcaaggcc agtaccgcgc
     5101 ccgccgcatc cagtatcaaa aaggggggcg tatataagat atttgcaaac agcttggatt
     5161 tgtcggtatg ttcttcagta accgtgtaat aaatatcggc aggcacactt tgctcaaaat
     5221 ggtaatcggc gttcagtttt tgcggtgtgg cgtagtattt gcctttggcg gatacgcagc
     5281 gcgtgtaaat ggtccgattg tcgagtttga ccgcttcaaa cccgagctgt ttcagcttgg
     5341 cgatgtcggc aggcttgtcg gtatcgtagc gcaggcaaag gccttcggta ctgaaattct
     5401 ggctgccagg cgattcgagt ttgaccggca gggcttggtg gcgagcatag ctcggggtat
     5461 cctcaactat ttggaagggt ttgtccagcc ctgccttcaa aatgcccgtc agcttcgccg
     5521 aatcttcggg attgacgacg aaccagtatt ttccgcccat catcaccagg ctgccctttt
     5581 ccaattgggc attgtcttcg gcaaccacac cgaaggcgcg gatttggtct ttgtcaacgt
     5641 gtttgcgggt gattgtttcg ctgaccgggt tgttcattcc ccacaacatc aacgtacagc
     5701 cgttcagcat caaggttgcc gccaaaacgg cggcggtcgt tttacggaac aacattttat
     5761 cctccaatct aaataacggc ggatgtcgtc tgaaaatatg cctaaatttc cgcgtccccg
     5821 ttttccatca cggttttcaa ctcttgaaac agggcgcgga aattttttgg tggtttgttt
     5881 tgctcctgct cttttttggt attgcggatg agtgtcctca gcttgcccgc gtccgcatgc
     5941 ggaaaatccg acataaactg cgtcaacgcg ccgtcgtctg ccaacagccg tacgcgcgcc
     6001 tgttccacgc gttgcaaaaa ggcgttgtgc gccgcatcgt cgccgcgcag cttggcaaga
     6061 aacgcctcga tgggcgcggg atcggtatcg cgcatcagcc tgccgataaa ttgtgcctgg
     6121 cgtttgagcg cgccgttgga tgtgattttt ttataggcgg ttaccgcctc gtacaaatcc
     6181 gcatccaaac cgattttttt cagcgtatcg tttgagagct tggtcaacgc catacccaaa
     6241 tcctgcaaat cgttcatctg ctttttcatt tgggttttgc tgatccattc gtcttcttgt
     6301 tcaaacatct tgattctcga atttttccaa tagcgcattt taacagaatg ccgccgcgtt
     6361 cagacggcat tttgcaccgt ccgccctgca aacggcgcgc ttttgcccct gccgcccgaa
     6421 acggttaaaa tgccgcaaag catcatcaag aaagatttct atgctgttca accacaccgc
     6481 ttccgaactc ctcgacctct gccgccgcac gctcgacttg gcaaaagcga cgggcgcaac
     6541 cgccgccgaa gccgatttca gcgaatcatt gggacaaagc gtcagcgtgc ggctgggcga
     6601 aatcgaacaa atcgagttcc agcaggacaa gtcgctggac attaccgttt acgtcggcaa
     6661 acgcaaaggc cgcgccagta ctgccgactt ctccgaaaaa gccctgcaag acaccgtcaa
     6721 agccgccatc gacatcgccc gccacaccgc cgaagacggt tgcgccggac ttgccgatgc
     6781 ctgtctgatg gcaaaacaca tcggcgaccc cgacctttac cacgaatggg atttggatac
     6841 ggaagccgcc gtcggcttgg caaaacaatg cgaacaagcc gccctgaacg aggatgagcg
     6901 catcgaaaac tccgaaggcg cggcggtgca aaccggccat taccaatacg tttacggcaa
     6961 cacccacggt tttgccgcac accggcaagg cacgcaccac agcatttcct gcagcgtcgt
     7021 tgccgccgac gaaaacggca tgcagcgcga ctactggtac gattccgcct gccgccatcc
     7081 ggatatggac agccccgaaa ccatcggtca aaccgccgcc cgccgcactt tgcgccgact
     7141 cggcagccgc agcatcccga caggcagcta ccccgtcctc ttcgatacca ccgtttcggg
     7201 cggtcttatc ggacacctcg tcggcgcact ctccggcggc gcgctctacc gccaaagcag
     7261 tttcctgatc gacagcatcg gcaaaaaagt cctgcccgat ttcctcaacc tgcgcgaaga
     7321 accgcacatc ccccgctctt tccgcagcag ctattttgat gcggaaggcg ttgccaccgc
     7381 accgcgcttc gtgattcaaa acggcattgt caaaggctat ttcctcagca gttacagcgc
     7441 gcgcaaactc ggtatgcaga ccacgggcaa cgccggcggc gcgcataacc tgtatttgaa
     7501 ccatacgcac gaaacgcaat ccgacctgct gaaagaaatg gggacgggat tgttggtaac
     7561 cgaactgatg ggacagggcg cgaacaccat taccggtgac tactcgcgcg gcgcggcggg
     7621 tttttgggtg gaaaacggcg tgattgccta ccccgtccac gagattaccg tagccggacg
     7681 cttgcaggat atgtaccgcg acattgtcgg cgtggcggat gacgctttgc ggcgttcgtc
     7741 caacaaaatc ggctcgattc tgattgcggg gatgacggtt gccggaagct gaacgccctg
     7801 ccgatgccgt ctgaagacga aggcagcacg ttttgccccg tgcctgcccg tttgccgttt
     7861 tatgccgcac cgccttttgc cgacaacatt gcacattttt tcatcagcac tgattttccc
     7921 ttatccgtca aaatactaag ccacttgtcc acaacagggt acacacaagt tcaggataag
     7981 gcgtgcgggg attggggttt ttccgccgcc ggcaaa
//
LOCUS       DAWIVB010000090         8005 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWIVB010000090 DAWIVB010000000
VERSION     DAWIVB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.090"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            447..1655
                     /locus_tag="WIX60_001321"
     CDS             447..1655
                     /locus_tag="WIX60_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ5305587.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
     gene            1676..1987
                     /locus_tag="WIX60_001322"
     CDS             1676..1987
                     /locus_tag="WIX60_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305588.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            1991..2191
                     /locus_tag="WIX60_001323"
     CDS             1991..2191
                     /locus_tag="WIX60_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305589.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            2429..2659
                     /locus_tag="WIX60_001324"
     CDS             2429..2659
                     /locus_tag="WIX60_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305590.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            2688..2987
                     /locus_tag="WIX60_001325"
     CDS             2688..2987
                     /locus_tag="WIX60_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305591.1"
                     /translation="MGFRVGMNCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGEKW
                     VLNGKPVMLSYPKCSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGA"
     gene            3106..3426
                     /locus_tag="WIX60_001326"
     CDS             3106..3426
                     /locus_tag="WIX60_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ5305592.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            3410..4915
                     /locus_tag="WIX60_001327"
     CDS             3410..4915
                     /locus_tag="WIX60_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ5305593.1"
                     /translation="MCCSLSFAEPVRIDDRIIKFRPSKSKFFESTGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAAVLSGVGKLVRQGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFVKGYEYSNCLWYEDERRINRNYGCYGVDSSIMRL
                     MSDYSRFPEVKELMESQMERLARPYWEKLRNRPDMSYFKNYNFKRCYFGLNGGDCLVA
                     KGDDGRTFINFSLQGNSKYKEEMDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEV
                     APGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPL
                     PEVSPAENPANNPNPNENPGTSPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPGRTNG
                     RDGKDGGLLCKFFPDILACDRLPESNPAEDLNLPSETVNVEFQKSGIFQNSAQCPAPV
                     TFTVTVLDSSRQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            5219..5503
                     /locus_tag="WIX60_001328"
     CDS             5219..5503
                     /locus_tag="WIX60_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ5305594.1"
                     /translation="MKLLAALIPLLMSVVGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            5504..6691
                     /locus_tag="WIX60_001329"
     CDS             5504..6691
                     /locus_tag="WIX60_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ5305595.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGSKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLSE
                     KPESKPIYNGVRQVRTFEYIAGCVEGGRTGCTCYSHQGTALKEITKEMCKDYARNGLP
                     FNPYKEESQGRDVQQSEQHHSDRPQVATLGGKPWQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            6906..7862
                     /locus_tag="WIX60_001330"
     CDS             6906..7862
                     /locus_tag="WIX60_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ5305596.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         2539 a         1595 c         1835 g         2036 t
ORIGIN      
        1 atatgatgcg ctctatcaaa ggggcgcatt acttttctta acattcccct ttgacagcca
       61 agtgaaaggg gcttttttat gtcagtagca aatgtaatat tttcttgttc ctattggaga
      121 atatttaaaa aatcagatta ttgcgtttta tgtttctatc agttcaggca tggtgaaccg
      181 cataaactcg ctgaacaaga aaatttttca aagctttatc aggcgttcga ttatatagat
      241 tcggttggct cgaattttcc ggtaattatc acaacagacg gttgtggtct ttcttcttga
      301 tctttaacag tttgtcagga ttgggctttc ggtcgttgac cgttggacgc gctttagcgc
      361 ggcagacggg aaacggctga aagccccccc ccctgactaa caggggggga gcgaaataaa
      421 acaaatccct aaaggtactg aacaaaatga gtgaagcaga atatttttcc catttcatat
      481 ctaacggcaa tgggaagtta ttagaaattc cgcaacgtag gggcaggcag gacggcgttt
      541 ttatagattg gttgtcattc acactgcacg aagattcctt gctgaaagtt tccggatgcc
      601 ccttagtttc cgatgccgaa tatatgtttg ttttaagcaa aaaattggag gaaatattag
      661 ggtttggcat cacgagcaga tgcaaatcga agggcaataa attttacgat tcgatgttta
      721 ggttgggatc ggaagaagtt gactacggcg aagtccatta cggaggtcag cgaaatacgg
      781 ttttaatcga attgaaaggt gtaggttgca acattgcaaa tccaggttgg gaattgaggc
      841 ttaagcagtt tttggaagat tcattgaggc cgaggataac gcgggtagat ttggcacttg
      901 atttttttga tggggagtac acgccggaac aggcactttt ggatcacgat aacggttttt
      961 tcgataacag taacatgagg ccgaaatctg aaatggttgg aacggcttgg cggagagagg
     1021 acgggagcgg caagacattt tatgtaggtc gcaagaaaaa ttctcgtttt gtgcgtgttt
     1081 atgagaaagg caggcagcta ggcgataaag aaagcaagtg ggtaaggttc gaaattcagt
     1141 ttaatcatgg agatatggaa atacctttgg atattctgat aaatcaaggt tcttactttt
     1201 caggcgcttt cccgatttgt cagaaattta aaaatatgcc gaatccggaa aggttcgatt
     1261 accgtaaaaa agtggctaat ttaacttttc agcataaatt gagatacgca aaaaacgcgg
     1321 tcggcaaact gattaatttc atgtttgata tgggttttga tagtgatgaa attgtcagat
     1381 atctgaaggc agatttgggg tatcccaaag ggctagaacc tgaaaaatat tcgttggccg
     1441 gattgaagga atctttgaaa ttcggcttta tccacgaaca accggatgta gatttagagg
     1501 ttgaattgga agaactcgga attatcaaat ttaagcaatc agataaattc gatccggata
     1561 aaaggctttt cgatccacat cacgatgtag aaagtgagag gcaatatcag ctttatctcg
     1621 acagaatgta tgatcttcat gcaaatcaaa attaacctaa aaaggaaaaa ttaatatgtt
     1681 taatcaaact caaactgtaa cttatcccgc aactttttta ggagctaaaa aattcaaagg
     1741 cgaaattgat ggctctaata tcgacacttg ttccgtattg gttgcaacac ctttgccggc
     1801 acagtcggga aatgctgttg gattcacggc agcacaaatg aagttcgggg acagtaagaa
     1861 tttctcaaaa ttagagaatc tcaaataccc gtgcgaagtt atggtaacgg ttgaaatgac
     1921 ttcgacaggt aagggcatgg ttccttcatt aattgatttt caggtggcag aaaagccgaa
     1981 aggttgattt atgaaatttg aagaacgttt catagttcaa gacttggaaa cgcatgactt
     2041 tatttatccc gatcctttcg gtgatgtggg gtttactcaa aatattaaat cagcaggtca
     2101 atttgaaagc tacgaagatg cgttgaattc aggcataaat gaaataggcg gaggattcca
     2161 gatatttcag ttcttcgtaa aatcggaata aaagaaaaac aggctcggcg ggcggtctgt
     2221 caacctttca caaagcccgc aacaaaggaa aaatatcatg aaaatgaacc ttgcaacact
     2281 aattatcggc tgggtggtct gtatgtttct ttttcttttc gccatcctct attttatcgg
     2341 ctaaaaacga gattcgggaa agacttcgcc cggatgaagc aagtcaagaa gtcgtcttac
     2401 tttaaacatc aaaaaaagga aaaaaacgat gaacatcgtt aaaaaatatg ctgtaaaagc
     2461 attgttggca gccggtatct tcacaccggc tattgttatg gcagatggct ttgatgcatc
     2521 cgcgattggt acgcaagtgg cgaatgtaat catgggtttt gtggcaatgg tttccgccgt
     2581 gggtatggcg gcaattaccg tgattcttgc aatccaaggc ttcaaaatgg cttggagcat
     2641 gatcaaatct gtcaaataac ggcagtgaag aaagaggggg cgcgtaaatg ggctttcgtg
     2701 tcggtatgaa ttgttttgat acaagattgc aggcagacga ctatttattg tcgtcccttc
     2761 cccctactgt tacccaggac ggaaaaatca tcaggccgga aagggtgggc gaaaaatggg
     2821 tcttgaacgg aaagccggta atgttgtcct atccgaaatg ttccaatttt gagcagatca
     2881 aacagggttc ttatgtcggt tcgacggttt taattctgtt tgtcgttatt tacggcttca
     2941 ggctgctgat taatttctta aaagacatag gcaaagtagg ggcgtgatga tgtttgtcga
     3001 tttttggttt ttgctcggat ttttcctggc tttgtctgtc gctttgatat ttatatgacg
     3061 tgttttaaaa tcaggctttc aaaacaacct ttgaaagaca gaaacatgaa caagccgttt
     3121 atcacgcagg cgcagttggc actttataaa tatcagccgt ccagcaagta ttttgggcag
     3181 tcgatggcat taattgcgtc taaggaattt gaagagtttg taagaaatgt aaaagaatac
     3241 gacgtaatag aatgtttctc ttatttttta aataagaggg taacgcataa tatttggaaa
     3301 atttattttt ctgatgagtc taatattttt attaggaagt cagaagaaaa tggaaaaatt
     3361 tcgcatgaat ttatttactc ggaattttct gatagcaaca ccgattttaa tgtgttgttc
     3421 tcttagtttt gcagaaccag taagaataga tgatcgaata ataaaattta ggccatctaa
     3481 atcaaagttt tttgaatcta caggatatag aaaaatcaat aatgaatttt ctaaattcac
     3541 agaagcggca aatgtcgaac atatccccac gggcgcaaaa gcccgaatca acgcaaagat
     3601 aaccgccagc gtatcccgcg ccgccgtctt gtcaggagtc ggcaaacttg tccgccaagg
     3661 cgcgaaattc agcacaaggg cagtccctta tgtagggaca gcccttttag cccacgacgt
     3721 atacgaaact ttcaaagaag acatacaggc acgaggctac caatacgaca ccgaaaccga
     3781 caaatttgta aaaggctacg aatatagtaa ttgcctttgg tacgaagacg aaagacgtat
     3841 taatagaaac tatggctgct acggcgttga cagttccatt atgcgcctta tgtccgatta
     3901 cagcagattc cccgaagtca aagaattgat ggaaagccaa atggaaaggc ttgcccgtcc
     3961 gtattgggaa aagttaagga atcgtcctga tatgtcttat tttaaaaact acaattttaa
     4021 acgttgttat ttcggattga acggcggaga ttgtttagtt gctaaaggtg atgatggtag
     4081 aacttttatc aatttctcac ttcaaggaaa ttcaaaatac aaagaagaaa tggatgccaa
     4141 aaagctggaa gagattttat cgttgaaagt cgatgccaat cccgacaaat acataaaggc
     4201 aaccggatat cccggttatt ccgaaaaagt agaagtcgca cccggaacaa aagtgaatat
     4261 gggacccgtc acggacagga acgggaatcc cgttcaggtt gtcgcaacat tcggcaggga
     4321 ttcgcaaggc aacaccacag tggatgttca agtaattccg cgtcccgact taacccccgg
     4381 aagcgcggaa gcgccgaacg cacagccgct gcccgaagta tcgcccgccg aaaaccccgc
     4441 aaacaacccg aaccccaatg agaaccccgg cacgagcccc aatcccgaac ccgaccccga
     4501 tttgaatccc gatgcaaatc ccgatacgga cggacagccc ggcacaagac ccgattcccc
     4561 cgcagttccg ggccgcacaa acggcaggga cggcaaagat ggcggccttt tgtgcaaatt
     4621 cttccccgac attctcgctt gcgacaggct gcccgagtcc aatccggcag aagatttaaa
     4681 tctgccgtct gaaaccgtca atgtagagtt tcagaaatca ggaatctttc aaaattccgc
     4741 acagtgtccc gcacctgtca ctttcacagt gactgtgctt gattcaagca ggcagttcgc
     4801 gttcagcttt gagaacgcat gtaccatagc cgaacggcta aggtacatgc ttctcgccct
     4861 tgcttgggcg gttgccgcct ttttttgtat ccgcacagta tctcgtgaag tctagcaggc
     4921 gcagcaccgc cgggcttcag taacttgtac caaggcaggg ggaggacgtc cagaaagatt
     4981 tgtaaagacg gctttatcgt ctttataaat ctttttggat accccttgcc gccccgccaa
     5041 aagaatacac tctgccgcaa gggcaggtgg taaggcgcgc gctttttgcg ccgttccccc
     5101 tgcccccgcg gcgtcgcaag tgagactggg ggtgtggggg ctagtccccg caaaatcttt
     5161 cagattaaga aacatttgtt taatgaggca accgtgcctt ttaagaaagg gatagcaaat
     5221 gaaattgttg gccgcattga ttccgctctt gatgagcgtc gtaggccgta tattgactgc
     5281 attgggattg atggccgtga cctattcggg ggtggataga ttggtagccc attttcaaca
     5341 ggcgataacc catagcataa cgggcgcacc tcaagcaatg ttacagcttt tctatataag
     5401 cggcggtggt actgttctaa acattctttt cggcgcgatc gcctttattc tgtcattcaa
     5461 acaaatgaca aaactagcaa cctcaatcgg gaagaaaaaa taaatggcag agatctgttt
     5521 gataaccggc acgcccggtt cagggaaaac attaaaaatg gtttccatga tggcgaatga
     5581 tgaaatgttt aagcctgatg aaaacggcat acgccgtaaa gtatttacga acataaaagg
     5641 cttgaaaata ccgcacacct acatagaaac ggacgcaaaa aagctgccga aatcgacaga
     5701 tgagcagcta tcggcgcatg atatgtacga atggataaag aagcccgaaa atatcgggtc
     5761 tattgtcatt gtagatgaag ctcaagacgt atggccggca cgctcggcag gttcaaaaat
     5821 ccctgaaaat gtccaatggc tgaatacgca cagacatcag ggcattgata tatttgtttt
     5881 gactcaaggc tctaagcttc tagatcaaaa tcttagaacg cttgtacgga aacattacca
     5941 catcgcttca aacaagatgg gtatgcgtac gcttttagaa tggaaaatat gcgcggacga
     6001 tcccgtaaaa atggcatcaa gcgcattctc cagtatctat acactggata aaaaagttta
     6061 tgacttgtac gaatcagcgg aagttcatac cgtaaataag gtcaagcggt caaagtggtt
     6121 ttacactctg ccagtaatag tattgctgat tcccgtgttt gtcggcctgt cctataaaat
     6181 gttgagcagt tacggaaaaa aacaggaaga acccgcagca caagaatcgg cggcaacaga
     6241 acagcaggca gtacttccgg ataaaacaga aggcgagccg gtaaataacg gcaaccttac
     6301 cgcagatatg tttgttccga cattgtccga aaaacccgaa agcaagccga tttataacgg
     6361 tgtaaggcag gtaagaacct ttgaatatat agcaggctgt gtagaaggcg gaagaaccgg
     6421 atgcacatgc tattcgcatc aagggacggc attgaaagaa attacaaagg aaatgtgcaa
     6481 ggattacgca agaaacggat tgccgtttaa cccatataaa gaagaaagcc aagggcggga
     6541 tgtccagcaa agtgagcagc accattcgga cagaccgcaa gttgccacgt tgggcggaaa
     6601 gccgtggcaa aatcttatgt atgataattg gcaggagcgc ggaaaaccgt ttgaaggaat
     6661 cggcgggggc gtggtcggat cggcaaactg aagaaaacgg caagagagaa aaaagacccg
     6721 taaaccgttt gaatatagac ggcttacggg tctttgtttc gcgcaaagca agggctaagg
     6781 cagtcaggca gcaaatcccg caatgtatta aaacagacgc gtagaaatgc cggctgcctt
     6841 tatccatcct caaaattgaa tatcatccta gccgtatcaa ggctgtataa ataaggaaaa
     6901 taccaatgaa tataatcggg ctggacatct caaaggatac catagacgca acattgcata
     6961 aaacaaacgg aagtatccat tacattaaat ttaagaataa tgatgatgga ttaaaacagt
     7021 ttagattgtg gataaaggga aacagaatca gaaaagccta tatcggcatg gaggcaacag
     7081 gcatctatta cgaaaaggca gcagatatgc tttcttccta ctataccgtt tacgttatca
     7141 atcccttaaa aatcaaggac tacgggaaaa gcaggtttaa ccgtaccaaa accgacaaag
     7201 cagattcaaa cctgatagca gattacataa aaaggcatca agatacattg ataccgtatc
     7261 agatacccaa aaacaaagca ctgcaaaaac tgattaacct taaaaatcaa ttacagcaac
     7321 atcagaagca aattaaaaac cgtcttcata gcactgaaga agacttcata aggaacatac
     7381 atcaagactt gatagatacc atacaggaca agatggaaca ggtaaaaata gccatatccg
     7441 aacaaatcaa aaaacaaacg gacaataacc attaccgcaa tcttcaaacc atcccgagca
     7501 taggcaaaga caccgcatca gttctttatg cgcaactgac agaaaaacat tttaaaaccg
     7561 caaaccagtt tgtatcctat gccggattaa gtcccgccat catacaatca gggacaagcg
     7621 taagaggtcg gggcagattg agccgatacg gaaacagacg attaaaaagt acgctgtata
     7681 tgcccgccct ttgtgcttac cgttttaacg catttccgaa attaataaat aatctgaaaa
     7741 aagcgggtaa gccaaagatg gtaatcatcg ttgccatcat gcgcaaactg gcgaagctcg
     7801 cctattacat tgttaaaacc ggccagcctt acgatgcgga aagacaccga ttgaatcaat
     7861 aagattcaac aaaattaaac ggttacgcga atatatttgt gtaaccgtgc atttgcatat
     7921 cgtaaataaa cgtaaataaa aataacaata taaatcagta tattgcaact ttgtttttta
     7981 ttttgtgttg acgggcaaca tatca
//
LOCUS       DAWIVB010000091         7935 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWIVB010000091 DAWIVB010000000
VERSION     DAWIVB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.091"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(104..1027)
                     /gene="era"
                     /locus_tag="WIX60_001331"
     CDS             complement(104..1027)
                     /gene="era"
                     /locus_tag="WIX60_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ5305597.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1075..1794)
                     /gene="rnc"
                     /locus_tag="WIX60_001332"
     CDS             complement(1075..1794)
                     /gene="rnc"
                     /locus_tag="WIX60_001332"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ5305598.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGYANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKRKKK"
     gene            1961..2284
                     /locus_tag="WIX60_001333"
     CDS             1961..2284
                     /locus_tag="WIX60_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ5305599.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2332..2808
                     /locus_tag="WIX60_001334"
     CDS             2332..2808
                     /locus_tag="WIX60_001334"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ5305600.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2886..3311
                     /gene="nusB"
                     /locus_tag="WIX60_001335"
     CDS             2886..3311
                     /gene="nusB"
                     /locus_tag="WIX60_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ5305601.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3679..4713)
                     /gene="pyrC"
                     /locus_tag="WIX60_001336"
     CDS             complement(3679..4713)
                     /gene="pyrC"
                     /locus_tag="WIX60_001336"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ5305602.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4929..5075)
                     /locus_tag="WIX60_001337"
     CDS             complement(4929..5075)
                     /locus_tag="WIX60_001337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305603.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            complement(5076..5300)
                     /locus_tag="WIX60_001338"
     CDS             complement(5076..5300)
                     /locus_tag="WIX60_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ5305604.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5409..6020
                     /locus_tag="WIX60_001339"
     CDS             5409..6020
                     /locus_tag="WIX60_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ5305605.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            complement(6080..6952)
                     /locus_tag="WIX60_001340"
     CDS             complement(6080..6952)
                     /locus_tag="WIX60_001340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ5305606.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6990..7775)
                     /locus_tag="WIX60_001341"
     CDS             complement(6990..7775)
                     /locus_tag="WIX60_001341"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ5305607.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNTGNEAAAVGALVKELKDAVR"
BASE COUNT         1895 a         2395 c         1904 g         1741 t
ORIGIN      
        1 acagataata tcaacccgtt tttaacaaag atattcccga ttgaaacaaa atgccgtctg
       61 aaacctgttc ggacggcatt tgcgtaaagt tcagcaagaa aaactacaaa cccagctcgc
      121 gcaggaagcg gatgtcgtcc gcccaaccgg atttgacttt gacccagacc ttcaaaaata
      181 ctttggtatc aaacagtttt tccatatcca accgcgcttc ggtggaaatt ttcttcaaac
      241 gttctccgcc tttaccgatt aaaattgcct tttggctttc cttatcgacc aaaacggcga
      301 tatagatgcg gttcaaaccg tcttcctctt caaactgctc cacttcgacg ttcatcgcat
      361 aaggcaattc ctcgcccaaa tagcggaaca atttttcacg cacgatttcc atcgccaaaa
      421 aacgcgccga tttgtccgta accatatctt cgggatacat cggcacgctt tcgggcagat
      481 acggcttaat cagctccaac aggttggcaa tccgcaatcc gtgtttcgcg ctgaccgcct
      541 ccgccgccgc aaattcaaat tcggcgcgca cttgggcaac aaacgcctcc agcgcgtaac
      601 ggtctttcgc cttgtccttg tcgattttgt tgaccactaa aatgaccggc gtgtgcttgg
      661 gcagttgttt caacacgacg cggtcggcat cggtaaagcg catcgcctcc acgacgaaaa
      721 ccaccacatc cacgccgccg agcgcctcgg taacattttg attcagcctg tcgttgagcg
      781 cgttgcggtg gtcggtttga aagccgggcg tatcgacaaa cacgaactgc gcggtatcgt
      841 cggtataaat ccccgttacg cggttgcgcg tcgtctgcgc ctttttgctg gtaatactga
      901 ttttctgacc gatgagatgg ttcatcagcg ttgatttgcc cacgttcgga cggccgacaa
      961 tcgctacgaa gccgcaacgg tatccgccgg cggcgcgttc ccctgcaagg aaggtttcaa
     1021 tatccatatt tgctttcgta tccatatgtt cagacggcat attcacgcgc cgcctcattt
     1081 ctttttcctc ttcagcggca gcttctcttc cagccatttc aaagcctctt tcgccgcttc
     1141 ctgctccgcc gccttgcggc tcgtcccttt ggcacggcac acgaaaccca gttcgcccaa
     1201 atcgcaggaa atgacaaaca tactgtcgtt ggcataaccg atttgctctt cgatacggta
     1261 tttcggcaag gcgaaacggc gcgcctgcaa cgcctcctgc aaagcagttt tgccgtcttt
     1321 tgcctgattt tgaaaatcgg cgcgccggac gcgatcggca aacaaatggc gcaccacctt
     1381 ttccgccgtg ttgaaatcgg catcgaagct gacggcagca aacatcgcct ccatcgcgtc
     1441 tgccagtatc gaaggccgtc tgaagccgcc gctcttcaac tcgcccgccc ccaagtacag
     1501 gccgtcgccg acattcattt ccgccgccat ttccgccagc acgccctcat tgaccagact
     1561 tgcccgcaac cgcgacaact cgccctcggt caacttcgga aacgcgtcaa acagcatccg
     1621 cgccaccgta taattcaaaa tcgaatcacc gacaaactcg aaccgctcgt tgtgcttcgc
     1681 atgatggctc ctgtgcgtca aagcctgccg caaaagcgaa atatcgcgga acgcgtagcc
     1741 cagtttcttc tgtatcgccg cgtgtgcctg ctgtttcaaa acatcgtctt tcattaccgt
     1801 cttccgcaaa aaacagcccg aatgcgtttc catactccaa aaatacagaa acaccttaag
     1861 gctgcaaacg gcgcacattg taccaccaaa ccgccgccgt ctgccgacaa cgcacgaatc
     1921 gtggtaaaat ccgtaagttt tcccccaacg tattcccgta atgaacgcat ttgcacaagc
     1981 actttcctcg gcactcgacc gctgtatcgc caccaacacc gtcgccaaac aaaaccggcc
     2041 cgtcggcttc ctctaccgcg aagcccccgt ttttgaaaac gacagcggct ggcgtttctt
     2101 cagcggcgac gagaccgacg aatacaccga cgatccggac aacttcagca tcgtcagcct
     2161 cgccgacatc gccaaaacca accctgaaac cgccgcactg ctctcccaac ccgaaggcag
     2221 cgcatgggag cttgccgaag acggcacatt ccaaaccgtc gccgactggc agccgcaaga
     2281 ctgaaacctt cagacggcat tccgaaacct ccgaatacca aaggacaccc aatgaacacc
     2341 atcgccccca acctcgacgg caaacacctc cgcatcggca tcgtacaggc acgcttcacc
     2401 aacgaaatcg gcagcgaaat gctcaaagtc tgctgccgca ccctccaaga attgggcgtg
     2461 gcagacgaaa acattaccgt cgccaccgta cccggcgcgc ttgaaatccc catcgcgctg
     2521 atgaactttg cctcttccga aaagtttgac gcactgattg ccatcggcgt cgtcatccgt
     2581 ggcgaaacct accatttcga gctggtttcc aacgaatccg gagcaggcgt cagccgcgtc
     2641 gcactcgact acaatatccc gattgccaat gccgtcctaa ccaccgaaaa cgacgcgcag
     2701 gcaatcgaac ggattgaaga aaaagcctcg gatgccgcca aagtcgccgt cgaatgcgcc
     2761 aacctcgtca acctgctgct cgaagaacag tttgaagacg aagaataaca aacagggcgt
     2821 acaccaatac accctttccg tttcagacgg cataccgtgc cgcccaaccg tttcaaggaa
     2881 aaatcatgaa aacagcccgc cgccgttccc gcgagcttgc cgtacaagcc gtttaccaat
     2941 cccttatcaa ccgcaccgcc gcgcccgaaa ttgctaaaaa catccgcgaa atgtccgact
     3001 ttgccaaggc agacgaagaa ttgttcaaca aacttttctt cggcacgcaa accaatgcgg
     3061 cagagtatat ccgacaaatc cgcccgctac ttgacaggga cgaaaaagac ctcaacccca
     3121 tcgaacgcgc cgttttgctg accgcctgcc acgagctgtc cgccatgccc gaaacgccct
     3181 accccgtcat tatcaacgaa gccatcgaag ttaccaaaac cttcggcggc acggacgggc
     3241 acaaattcgt caacggcatc ctcgacaaac tcgccgccca aatccgcccc gacgagccca
     3301 aacgccgttg atgcatcaca aatttaaaga aagttctatg tccttatttc tgctttcttt
     3361 gtttcgtcat tcccacgcag gtgggaattt ggttttttga attttgggta tttcaaataa
     3421 attctgaaac ttcaatctcg tcattcccgc gcaggcggga atccggtttt ttgagttgct
     3481 gccgtttttg ataaactctg gtcgcatcga atttctggat tcctgccttc gtgggaatgg
     3541 cgaaatttga aaattgtggg aatttatcgg aaataaccga aacgtaacaa actcaaacgc
     3601 cgttgacgta tcaatcagtc agcaacacaa atgccgtctg aaagccctcc cttcagacgg
     3661 cattttgttt ccagcccatc aatactgcac cgtccagccg atttcgccgc ccgcgcgcat
     3721 cgggacaagt tcgccgtcgc cgtagggaac acttgcggga acggtttggc tttgtttgac
     3781 gagggtgatc gtgtcggtat tttcaggaat gccgtagaac cttgcgccgt tttttgaggc
     3841 gaaggcttcg agtttgtcca acgcgcctgc tttttcaaat acttcggcgt aaagctcgat
     3901 agcggtcatc gcactgaaca taccggcgca gccgcaggcg ttttctttgg cggatttggc
     3961 gtgcggcgcg gagtcggtgc cgaggaagaa tttatgcgcc ttctcgccgg taacggcggc
     4021 gaccaatgcc tgacggtggg tttcgcgttt gagtacgggc aggcagaaat gatgggggcg
     4081 cacgccgccg accaagaggt cgttgcggtt gagcaggagg tgttgcgggg taacggtggc
     4141 ggctacgttg tcgcctgctt ccaaaaccag gcgggcggct tcggcggtgg tgatgtgttc
     4201 gaacacgact ttaagattcg gcacttgcgc caaaacgggt ttcatcacgc gcccgataaa
     4261 ggcggcttcg cggtcgaaga tgtcgatttc ggggtcggtt acttcgccgt gaacgaggaa
     4321 caaaatgccc tgtttcgcca tttcttccaa cacggggatg agcttgaaca ggtcggttac
     4381 gccggaatcg gaattggtgg tcgcgccggc agggtagagt ttgaaggcga cgatgccggc
     4441 ggctttggct tcgcgtacaa gttcgggcgt ggcgttatca gtcaaataaa gcgtcatcaa
     4501 cggctcaaac gcgctacctt cgggcaacgc cgccataatg cgcgctttgt aggcaagcgc
     4561 gtcggctaca ctgacgacag gcggtttgag gttgggcata atgacggcgc gccccatttg
     4621 gcgggcggta taaggggcaa cggctttgag cgcgtcgccg tcgcgcaggt gcaggtgcat
     4681 atcgtcgggg cggataatgg tcagggtttg catggttgtt cctttttagg ttttggctgc
     4741 gtttgaatac ccggtcgatt ccatccgttt gccgggggaa agccggttat tccgtccggt
     4801 cggaaaccgg ttccattgga tgaaaggcat tttgtccgac tgattgggtt gcatcaacgg
     4861 aatcttcaaa cacagccttt agattttgat tgtgcttggg ttttgcttct ttgccgacgg
     4921 caaggcggtt aacagccatt ccggaacaaa tcggcggcga tacgcccgaa atgccgacac
     4981 tgcacgccgg acggtttgtt tttgaaacgg cacgcggcac atacgatatt caaaaacaac
     5041 gccgtcgggc gggacatccg catttcagac ggcatttatt tgtgttggac gaccagcgtg
     5101 aacacgccgt cctgttcgga agcatccaac agcacatgac cggtttggcg gcaaaaagcc
     5161 tcaaaatccc ccggtgcgcc gccgtcggtt gccagaacgg tcaacacgtc gccctgctgc
     5221 atttgcgcca aagccttttt cgcccgcaaa atcggcaggg gacatttcaa tccggttacg
     5281 tctaaagttt cgctgttcat cgtttgtacc gtttaaaaaa tgggaatcgg ggggagatta
     5341 taccccagtt gcttgcagcc ccctttgcca acccataaaa taacgccgtt ttcaatttgg
     5401 agaaacttat gcgccgcacc gccctcctgc tgctcgcact cgccgccggc acgtccctcg
     5461 ctgccggctt cagccaaaaa gacacgccga tcaacacccg ctaccgcgaa acgcccgaag
     5521 aggccgccgc ccgcgaattt aaagaacata ccgccaaact gccgccgctg cccgatacgc
     5581 actcggacgg ctggttcgac atttatgtgg atgaaaatta cggcaaacag ccgaaaatcc
     5641 tgctcgacag cctgcaaatt atgcccgcgc ccgacggcag catccgctac attctcaata
     5701 tccgctccga caaaggctac gacaacctga ctgccgaagg catattctgc gcccgcttct
     5761 ccatcaggtt cggcaacggc aaaccttcct cctataaagt gttcggctac ggcgacacgg
     5821 tcaaccgccg ctggatacag ccccgaaacg ccgaatggaa accgataggc ggtacattaa
     5881 gccgcaacga cgcactgcgc accgtcctgt atcaggcatt ctgcgagggc ggtgtacccg
     5941 ccgacacgaa aggtttggta caacgcctga aagaacgtgc cggacgctat gccccatcga
     6001 taaagccgca caacaaataa ccagacacaa aaataccgtc tgaaacatcg gtttcagacg
     6061 gtactcgatt catcagccat caggcggcgg aaactttgtc ctgacggcac aacagcgtaa
     6121 tcaaatcact gatgcgccgc ttcatatcgc ggcggtcgac aatctggtcg attgcgcctt
     6181 tttccagcag gaactcggcg cgttggaagc cttccggcag cgtttcgcgc accgtctgct
     6241 caatcacgcg cggaccggca aaaccgatca gcgcgttcgg ttcggcaagc acgacatcgc
     6301 ccaaaaatgc gaagctggcg gatacgccgc ccatagtcgg atcggtcaac accgatataa
     6361 atggcaggcg tttttccgtc agcaaatgca gcgcggcact ggttttcgtc atctgcatca
     6421 gcgagtttac accctcctgc atacgcgcgc cgccggaagc cgccacacag acgaacggac
     6481 aattgtcggc aaccgcccga cggatacctt gtacgaatcg ttcgcccaca accgaaccca
     6541 tcgaaccgcc gataaagcgg aactcaaacg cagcaacgac gacgggcagg ccgttcatct
     6601 tgcctttcat caccaccaaa gcatcatctt ccccggtcag cttgcgtgcc gcactcaaac
     6661 gatccggata ttttttgccg tctttaaact tcaaaggatc tgtcggtttg acatttccgg
     6721 caacttcctc cctgccatcc tcatccaaaa gcaggttcag gcgttgtcgt gccgacaacg
     6781 gattgtggtg gttgcatttc ggacaaacct gattgttctg ctgcaactcg gtagaataaa
     6841 cggttgccga acaagacggg catttgtgcc atagaccctc gggaacattg gaagaaccgt
     6901 cttttccgcg attcttgatt ttgggtggca ggattttatc taaccagctc atggacaact
     6961 cctaaaatat tcaggatgag gaaccgccgt cagcgcacgg catccttcaa ctcttttacc
     7021 aaagcaccga cggcggcagc ctcgttgcct gtattgtttt cgatttcttt cacaatccgg
     7081 ctgccgacaa taactgcgtc ggcaacccgg ccgattttgc gtgcactttc cgcattgctg
     7141 atgccgaaac cgacaccgat gggaatatcg atatactgat gcaaatactc tattttacgc
     7201 gaaacctcat ccgtatccaa acttgccgcg cccgttacgc ccttgagcga aacataatag
     7261 acaaatccgc ctgccagctc ggcaatggtt ttaatacggt cttccgtcgt cgtcggcgca
     7321 atcaggaaaa tacagtcgac cccgttatcc ttcagctcgc gatagagcgg atcgatggtt
     7381 tcgacagggg aatccaccgt caacacgccg tccacacccg cctttgcggc ttcctgagca
     7441 aactcccgat aacccatctt atgtacaggg ttcaaatatc ccatcaaaac aaccggcgtt
     7501 tgcgtgtcgg tttcacggaa ttttctgacg acatccaaga catcgcgcag cgaaatcccg
     7561 tttgccaacg cccgctccgc cgcacgctga ataaccggcc catccgccat cggatcggaa
     7621 aacggcacac ccaactccaa aatatccgca ccgtttgcaa ccatgccgtg catcaatgcc
     7681 aaagttgtcc gaatatcggg gtcgcccacc gtaatatagg gaatcaatgc ctttccgcca
     7741 tccaaagcgg caaaagcctg ccggattctg ctcatttgtc tcttccatcc tatcggcaaa
     7801 tatgccgtaa aacacaatca gtaaattttt cccagtataa catttgcggt acggataggc
     7861 gaatacggca aacagaaacc gcgcaaatca gagccgccgt ttgaacgagg cggaaataaa
     7921 cggcaagcat ctcgg
//
LOCUS       DAWIVB010000092         7906 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWIVB010000092 DAWIVB010000000
VERSION     DAWIVB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.092"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(51..2093)
                     /gene="recG"
                     /locus_tag="WIX60_001342"
     CDS             complement(51..2093)
                     /gene="recG"
                     /locus_tag="WIX60_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ5305608.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2179..2622)
                     /gene="secB"
                     /locus_tag="WIX60_001343"
     CDS             complement(2179..2622)
                     /gene="secB"
                     /locus_tag="WIX60_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ5305609.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVEMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="WIX60_001344"
     CDS             complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="WIX60_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ5305610.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3113..4606
                     /gene="rng"
                     /locus_tag="WIX60_001345"
     CDS             3113..4606
                     /gene="rng"
                     /locus_tag="WIX60_001345"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ5305611.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4641..5891)
                     /locus_tag="WIX60_001346"
     CDS             complement(4641..5891)
                     /locus_tag="WIX60_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ5305612.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADDDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALPLQAER
                     LNNMPLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDEIPLTKTTFGLQSRFRQV
                     LQELMPLALEKCQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGKGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELSDSRRFGRGLLIRALLDKETLK"
     gene            complement(5887..6062)
                     /locus_tag="WIX60_001347"
                     /pseudo
     CDS             complement(5887..6062)
                     /locus_tag="WIX60_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6285..7598)
                     /locus_tag="WIX60_001348"
     CDS             complement(6285..7598)
                     /locus_tag="WIX60_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ5305613.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1858 a         2341 c         2058 g         1649 t
ORIGIN      
        1 aaatcagatt tccgttacac ttttttccaa tatttcagac ggcattttgc tcacacgccc
       61 aaataccctt ccctgccgga aagccacctt gccaaatgcg cttcgacgat ttcggggttt
      121 tgttcaatca gcatcggggc ggtttcgcgc gcttgttcca agaggtgcag gtcttcttcg
      181 agcttggcga aacgcagcat aggcacgccg ctttggcgcg cgccgagaaa ttcgccgggg
      241 ccgcggatgt tgaggtcttg gcgggcgatt tcaaagccgt cggtgtgttc gtagatgact
      301 ttcagccgcg ctttggcgag ttcgcccaag ggttcggcaa acaggaggac gcacacgctt
      361 tctgccgcgc cgcgcccgac ccgaccgcgt aattggtgca gctgcgccaa gcccatgcgc
      421 tcggcgtgtt cgatgaccat cagggcggca ttgggcacat ctacgccgac ttcgatgacg
      481 gtggtggcga ccaagacgtt cagccccccc gaagaaaacc gcgccatcac ttcggccttt
      541 tcggcggcct tcatgcgccc gtgtaccagt ccgatattga gttcgggcaa tgccgtctga
      601 agccgggcga gggtttcggc ggcggtttgc agttgcaggg tttcgctttc ttcaatcaat
      661 gggcagaccc aatacgcctg ccgccctttt cggcaagtgc cgaggacgaa gccttcgact
      721 tcggcgcggc ggacgttgtt gacgaggcgc gttttaatcg gtgtgcgccc gggcggcaat
      781 tcgtcgatga cggacacgtc caaatcggcg aaaaaactca tcgcaagcgt gcgcgggatg
      841 ggcgtggcgg acatcatcag ctgatggact tcgcgccctt tgtttttgag ggcgaggcgt
      901 tgggcaacgc cgaaacggtg ctgttcgtcc acaatggtca agcccaaatt gtgaaacgcc
      961 acgccgtctg aaaacagggc gtgcgtgccg acggcgattt tgacgctgcc gtcggcgagt
     1021 ttggctttgg cttcgtcttt ggctttttta cgcaaactgc caaaaaggcg gacaacttca
     1081 atgcccaaag gttcgagcca ttgtttaaat ttaataaaat gttgttcggc aaggatttca
     1141 gtgggcgcca ttacagccac ctgcgcaccg gattcgatag ccgtcaaagc agacaaagca
     1201 gccacaatgg ttttgccgct gccgacatcg ccctgcagca ggcggtgcat cgggtaggtt
     1261 tgcgccatat cgcggcagat ttcggaaaca actttttctt gcgcatcggt cagggcaaac
     1321 ggcagggctt ggcgcagggc ttgggtcaat gtgccgtcgc cgcccaatgc cgccgccgtg
     1381 ccgccgatac gcttctgtcg cgccaagcgc atcgaaagct gttgcgccaa aagttcatcg
     1441 aatttgagcc gttgccatgc aggcagcgtg ccgtctgaaa gctgatgaat cgtgaaactc
     1501 ggcggcggcg aatgcaaaag acgcaggctt tcggcgaggt gtggcagctt cagacggcac
     1561 agcagggcat cgggcagcgt gtcgtgcagc ggcgtaacgt ccaacgccgt ctgaataata
     1621 cggcgcaaag tgggctggtt caaaccgttt acggtcgggt aaaccggcgt gaggctttcc
     1681 gccaaaccgc cgccctcggc atcgcggatt ttgggatgaa tcatctcgtc gccgtaaaag
     1741 ccgtgtttga tttcgcccac ggcgcggatg cgtttgccga ccgccgtctg tttctgatgg
     1801 ctggcgtaaa agtggatgaa gcgcagaaaa aggacgctgc cggagccgtc ggcgatttgg
     1861 acaatcagct gcttgcgcgg tttgaacgtt acttcctgat ggataacctc cccctcgacc
     1921 tgacacggca cgccaatcgg cgcgtcctta atcggcataa tgtgcgtctc gtcctcgtaa
     1981 cgcagcggca ggtgcaacac caaatcccac gcggtatgga ggttgagttt gtcgagcttc
     2041 ttggcggaaa catcggtgat tttgagctgt tttcgggttt cgggcgacat cataggcaga
     2101 ttcctttgga cgcgcctatt ttatccgaaa acaaaaatgc cgtctgaaac ggattcagac
     2161 ggcatcgaca ggcaggaatc aagccccggc ggcttcggct tcctgctgtt gttggtaaat
     2221 cgcctcaaaa ttaatcggcg cgagcaggac gggcgggaag ccggcgcgcg ttaccgtacc
     2281 ggaaacggct tcgcgcgcgt aagggaagag gatgttcgga cacgccacgc cgagcagcag
     2341 gtcggcatct tcttcgggga tgttttccaa acggaaaata ccgctttggg ttacttcgtt
     2401 caaaaacatc gtgcgctcgt tatccaattt ggcggttacg gtaacggtta catccacgtt
     2461 gtagtagccg tcttccagct tttggctgcc ggtggaaacg cgcatctcca cttcgggctc
     2521 gccctgttcc aaaaagattt gcggcgcgtg cggcacttcc aaagacaagt ctttgacata
     2581 cagtcgctcg atgctgaata cgggttgcag ttcttcgctc attttgtttt cctagttggg
     2641 ggttaagggt tcagcagtcc gtccagcccg ccttcctgct ggaggcggta gaggtcggta
     2701 aatccgccga cgtgcgtttc gccgatgaaa atctgcggca cgctgcgctg tcccgaaagc
     2761 tgctgcattt cggcaaaggc ttcggggctt gcatcgacac ggatttcgtc gatatgtccg
     2821 acacctgccg cgtgcagcag ccttttcgcc atcgcgcagt aggggcaaaa cggacctgtg
     2881 tacatggtaa cggtctgcat attgggtttc cgaaagtttt gcaatgataa tcaatatagg
     2941 ggcatttccc ctgtttggca agtgcggaac agatgcacgt tcaaacggca tgtgcggaat
     3001 gtgtcaaagt ttctttttta aagtatgata gacattgtga aaaatatttt tgcacccgcg
     3061 ctgcgcggcg gaacggatgc aaaatatttt tattacattt tcaggaaaaa ccatgttgtc
     3121 aggactcccc atccccaaag acatcgcgcg cccgcccgaa acgatattgg tcaacatcac
     3181 gccgcaggaa acgcgcgtag cggtgttgga ggaaaacaat atctgcgagc tgcacatcga
     3241 gcgcaacagc gaacacagcc tagtcggcaa tatctatctg ggcgtggtgc gccgcgtgct
     3301 gcctgggatg cagagcgcgt ttatcgacat cggcttggaa cgcgcggcgt ttttacacat
     3361 cgtcgatgtc ctcgaacaac gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct
     3421 gtttgaaggg cagtctgttt tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc
     3481 gcggctttcc acccaaatct cgctggcggg gcgtttcctc gtccatcttc cgcaagaaga
     3541 ccacatcggc gtgtcccaac gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg
     3601 cctcgacaag ctcctgccgg aaaatgcctg ccggggctac atcatccgca ccaacgccga
     3661 aaacgccacc gacgaacagc tccagtccga catcgactac ctgaccaaag tgtgggaaca
     3721 catccaagaa caggcgaaaa tccggccgcc cgaaaccctg ctttatcagg atttgccttt
     3781 aagcctgcgc gtgttgcgcg atatggtcgg ctgcgacacg caaaaaatcc tcgtcgattc
     3841 caccgtaaac cacgggcgca tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg
     3901 caggatagag ctgttcaaag gcgaacgccc gctgtttgaa acccacaacg tcgaacagga
     3961 aatcagccgc gccctgcaac cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga
     4021 atccaccgaa gccatgacca cgatagacgt gaacaccggc ggcttcgtcg gcgcacgcaa
     4081 cttcgacgaa accatcttcc gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt
     4141 gaggctacgc aacctcggcg gcatcatcat catcgacttc atcgatatgg cacaggaaag
     4201 ccaccgcgaa gccgtgttgc aggagcttgc caaagccctc gccttcgacc gtacccgcgt
     4261 taccctgcac ggttttacca gcctagggct ggtcgagctg acgcgcaaac gctcgcgcga
     4321 aaacttaaac caagtcctct gcgaaccctg cccttcctgc caaggcagag gccgtctgaa
     4381 aacgccgcaa accgtatgct acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta
     4441 cgatgccgaa agtttccgca tcctcgccgc ccccaacgtc atcgatttgt ttttggacga
     4501 agaatcgcaa tccttggcaa tgctgataga tttcatcggc aaaccgattt ctctggcggt
     4561 cgaaaccgct tacacgcagg aacaatacga catcgttttg atgtaaaaaa tgccgtctga
     4621 agccttcaga cggcatctgt ctatttcagg gtttccttgt ccaacaacgc gcgtatcagc
     4681 agaccgcgtc cgaaacgtcg gctgtcggac aattccaaat atccgccgta ttttttggca
     4741 agcgtgtcgg cgatggacag acccagcccc gtcccctgct gctccgttcc caaaatacgg
     4801 taaaacggat cgaggacacg ggcgcgttcg gattcgggaa tgcctttccc gttatcttcc
     4861 acccacacgg caagatattt cccttcgtcc gtgaaaccca aatctatcct gccttcgggc
     4921 ggcgtataac gtaccgcgtt gtcggcaaag gttttaatca gcgtatagat ttccgtttcg
     4981 tcggcagaca cttcgacatc gcctccgacc gccacgccga tgtcctgaca tttttccaaa
     5041 gccagcggca tcagttcctg caacacttgg cggaaacggc tttgcagacc gaatgtcgtt
     5101 ttcgtcagag ggatttcatc cgactgcgaa cgcgccaatg ccaaaagctg ttcgagcagg
     5161 tgtttgttac gccgtatgct ttgctgcaaa acggcaggct gccgcgccgc atcgggtggg
     5221 agcggcatat tgttgagccg ttccgcctga aggggaaggg cggtcatcgg cgtacgcaat
     5281 tcgtgtgccg cgtcggcgac aaaccgctga cggtggcgga tgtcttcatc cgcacgtttc
     5341 aaaagcaggt tgatggcggt tacgaaacct ctgatttcac tgggaatatt gtccacactc
     5401 aaagcagaca ggtcattgat tcggcgttgt tcgagacttt gcgacaattt gcggacgggg
     5461 cgcatggctt tgtgcgtaat ccacacggtc agcaaaatca tcagcggcag tgccgccaac
     5521 aggggcaaca cgctttgccg tgccgcatcc gccgccaaat cttcacggta ttcgttttcc
     5581 tgcataacgg caatccgtcc ctgctcggtc gtgcggatat agacgcggta ataatcgtcg
     5641 tcatcgtccg cctgaagcgt gtgcagaccg tccgccagat gcgcaggcag gctgacaaca
     5701 gggtcttcct gctgcggcat ctgtaccaaa atacgcgtat cgccgtcgcc ctcgggcaaa
     5761 gtttcgggtt tggaatcggg ggcgacgtac aatgccgcct gacggagcag gtcgtcctgc
     5821 aacgcttccg tttcgtggaa ggtttcgtag taggaaaaca tacctgcaag cattgccagc
     5881 ggaacaaaca tccaaaccgt cccgccccgg caaacccaaa tccagcagca tcaagtcata
     5941 aggctgggca gcggcagccg cgcgccgttt ttgacccaat ccaccgcata gccgccgtct
     6001 ttcaaacttg ccgacaccgc ctccgcaatc atcgcatcgt cttccaccag caaaacacgc
     6061 atcaactttc ccttcaaaat aaaccgtgcc tattctaaca ccccaaaatt agccgcaatt
     6121 tagcggtctt tacgcttgcc ggtatttttc aaaactgcag cacaaaaaaa ccgcgccggc
     6181 aactgccttc agacggcatt ggggcgcgat tgcaacacac gggcaggcag gcagagcctg
     6241 cgacagacca caggaacgat tcaggcttca gacggcttcg ccgtttacgg cagaggcacg
     6301 attcctgccg ctatcgaact ggccaatatc gccagcgaca aaccccacgc ccagaaaaac
     6361 gaataacgga tgtgtttgcc catcgacaat ttcgccaaac ccaagcccat ccacaaagcc
     6421 ggcgaaagcg gcgtaacaaa agtgccgacg atactgccga tcaacatcgc ataacctgct
     6481 gcttcgggcg ccacgcccgc ctgcgaggta atctgctcca caatcggaaa cagtccgaaa
     6541 taataagcgt ccgtactcaa aaccaactca agcggaatgc ccaacacacc gatggcaata
     6601 tgcagataag gcagcagcgc gtccggcagg atatgcacaa tgtctttgga aatcgcgtcc
     6661 aacatccccg cacccttcaa aatccccaaa aacgtacctg ccgccaaaat aatggacgcc
     6721 atcatcaccg cgccgccggc gtgggcataa atccgctcca tctgttcctg cgggctgcgg
     6781 taattcaaaa gcaacgccgc cgttgcagcc agcataaata cataacccgg tgggaagatg
     6841 cccgaaaaaa gcaggctcat cgccgccaaa aacagcagga cattccacca aaacagtttc
     6901 ggacgcgcca atttttgttc ttcttccgac aaaggcaccg gctttatcaa atccgccacg
     6961 gcgggcaacg cgcccaactc ccggacaatc cgcctttttt cacgcacacc caaaagcagg
     7021 gacagcgcaa ggataaacac cacaccgata atttgcaccg tcaacaaagg tttatacaat
     7081 tcgcccacat ctgcgcccaa cacgcttgca acccgcccgg tcggcccgcc ccacggcaga
     7141 aggttaatca atcccgcact ggaagtcagc agcaaaaaca gcaggtaagg attcatatgc
     7201 agacgcttgt aaagcggcaa aagggcgggg acgaccaata aaaacgtcgt cgcacccgcc
     7261 ccgtccaact gcgccaccac cgacaccaag accgtcccca cactcactgc cacgatatta
     7321 ccccgagtca gcttaatcaa accgcctatc atcggacgga acagccccac atcgttcatg
     7381 attccaaaaa acaaaatgga aaacataaac ataatcacaa tctgcatcac cgatttggtg
     7441 ccgcccgaat aaaattcttt taattgggat acatcaaacc ccgccagcaa cgccccaaac
     7501 agcggcacca agattaatgc gatgatgggc gacacttttt ccgtcagcag cagccatacg
     7561 atgaccccga taatcagcag tccgataaac gtcagcatca tttctccttt attttatttt
     7621 aaacagaaaa ccgaccgtgc aggcaaaacc gcccacagac gcgggataag ccctgcattc
     7681 tactttttta ttttgaaaca agtcaatcgg tcatttcctc ccatttacgc ctgccgccat
     7741 tcctgcatcc gtccgtcatt tcacagcggc aaccgatacg gaacaaccgg taaatcggta
     7801 tcgggacggc gcgggggcat tcatcccggt gcgccgattc aaacgaaacc gcccctatca
     7861 ttgcggagcg cggggcgtgc cgtacacgcg ggattttata gtggat
//
LOCUS       DAWIVB010000093         7874 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWIVB010000093 DAWIVB010000000
VERSION     DAWIVB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.093"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(60..135)
                     /locus_tag="WIX60_001349"
     tRNA            complement(60..135)
                     /locus_tag="WIX60_001349"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Glu,seq:ttc)
     gene            complement(855..2267)
                     /gene="trkA"
                     /locus_tag="WIX60_001350"
     CDS             complement(855..2267)
                     /gene="trkA"
                     /locus_tag="WIX60_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ5305614.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(2365..3888)
                     /locus_tag="WIX60_001351"
     CDS             complement(2365..3888)
                     /locus_tag="WIX60_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ5305615.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMNVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(4195..5001)
                     /locus_tag="WIX60_001352"
     CDS             complement(4195..5001)
                     /locus_tag="WIX60_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ5305616.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDTAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(5053..5304)
                     /locus_tag="WIX60_001353"
     CDS             complement(5053..5304)
                     /locus_tag="WIX60_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305617.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            5404..6165
                     /locus_tag="WIX60_001354"
     CDS             5404..6165
                     /locus_tag="WIX60_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305618.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIESGENADEAESGTNEPAVSENGENDAEETLMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            6310..7479
                     /gene="metZ"
                     /locus_tag="WIX60_001355"
     CDS             6310..7479
                     /gene="metZ"
                     /locus_tag="WIX60_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ5305619.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSAGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVAKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELLAQVAMYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLSDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            7566..7772
                     /locus_tag="WIX60_001356"
     CDS             7566..7772
                     /locus_tag="WIX60_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ5305620.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         1712 a         1928 c         2292 g         1942 t
ORIGIN      
        1 aattgcggga atgacgactc ataataaaaa acctgcttga aacaagcagg tttttgaatt
       61 ggcacgccca cggggattcg aaccccggtt gccgccgtga aagggcagtg tcctaggcct
      121 ctagacgatg ggcgcgtgga aacggacatt atacatactt atagaaattt agcaatacga
      181 tgccgtctaa acaaatgtca ggggggcgtt tgtctgatcg catcacaata taaaaacata
      241 taaaaaacat tggaatatca agcaattcat ttttttgcgt ggtaatgccg acaacggaag
      301 gcagctttat tttgaggcgc aaaggtcttc gatgcacacg atgcgctaga cttggcaggt
      361 caatatgggc gtgcgcagta tgttctgatg cggcggattc gcctgaaaac aagccggaac
      421 ggtcagatgc gttccggatt gtttttttgt cgggggggtg ccgctatatc aaaacaggcg
      481 ggcgatgccg tctgaaactt cgtcattccc gcgaaagcgg gaatctagaa cttttaaatt
      541 ttcagacggc ttttgaatat tgccgttgcc cgacgttctg gattcccgca acttttcgtc
      601 attcccgcga aagcgggaat ctagaacctc taaattttca gataaccttt gaatattgcc
      661 gctatcccaa ggtctggatt cccgcctgcg cgggaatgac gggtctttta taacctttga
      721 atattgccgc tatcccaagg tctggatgcc cgcttacgcg ggaatgacgg ggagggcgga
      781 tgccgtctga agcccaccta ccattatttc cgcttccata ccgcttggcg gcaatatgtc
      841 cggaatgggg cggtttatcc gaaaaagccc attttgacct ggatgagttt ttccagttcg
      901 ttcaggatgc gccggcgcga gacgaaaaag atgatgtggt cgccgtcttg gatgacggtt
      961 tcggtatggt gtcccataat ggtttcgccg gttccggcgc ggacgacggc ggcaatgtgg
     1021 cagccttcgg gccatttgat gccgctgatg cgcctgccga tgatggcgga agtttttttg
     1081 tcgccgtgtg cgacgacttc gatggcttcc gccgtgccgc gccggatggg gtggacggca
     1141 acgatgtcgc cgcgccggat gtgggcgagt atcgagccga tggtgatgag gtggggggag
     1201 acgacgatgt cgattttgtt gccttcgagc aaatcgacgt agcttgagcg gttgacgatg
     1261 ccgatgacgc gcttcgcgcc gaggtttttc gccaaaaggg cggacataat gttgctttcg
     1321 tcgtcgttgg tcagggcgca gaatacgtcg atttcgtcga tgtattcgtt gtcgagcagg
     1381 gtttcgtcgg ttgccgaacc ttgcaggacg agggtgttgt cgaggttttc ggctatccat
     1441 tcggcacggc gcggccggca ttcgatgatt ttgacgttgt atgcgtgttc gagctgcttg
     1501 gcgagacggt agccgatgtt gccgccgccg gcaatcatga tgcggcgggt gctggtttct
     1561 ttggggcgca attcgggtat gaccgcgccg atgttttcgg cggcggcggc aaataggatt
     1621 tcgtcgcctt cgatgatgac ggtttgcggc gcggggacga tgaggcggtt gttgcggtaa
     1681 acggcgcaga tttggcagtc ggccccgtcg ggcaaatctt gggcgatgtc ggcaatgctg
     1741 cgtccgacaa gcagtccgcc gcgccgcgcc tgtatgatga ccatccgcac gcggtcgtct
     1801 gcaaaacgta aaacctgcaa tgcgcccggg cagtctatca ggccggcaag ctgttcggta
     1861 acgagctgtt cggggctgat ggtttcggtt atgccgaata tggaaaggct gccgttttcg
     1921 ttgttttcga gcttggggct gaggtattcg aggtattcgc tggaacggac gcgcgcgatg
     1981 cggccgggga tgttgaacag gtcggcggca actttgcagg cgacgatgtt ggtttcgtcg
     2041 ctgcgggaga gcgcgagcag caagtcggca tcttccgcgc cggcgcgttc taatgtgaag
     2101 ggggatgcgc cgttgccgaa aacggtttgg acatcgaggc ggctgcctgt ttcctgcaat
     2161 gctttttcgt cgatgtcgat aacggttacg tcgttgttgg gtatggcggc aaggttttgt
     2221 gcgacggtag aacctacctg tccgttgcct aaaatgagga ttttcacggt gtgggtcgcc
     2281 gagtgatgaa atggtggatt gtaccgcaac cgggggcgga aatgccgtct gaaggcagat
     2341 tttgtgttca gacggcatgg cgcctcaaga ttcgacgggg atgatgccga ttttcgcctg
     2401 ccatttgcgc ggggcggtgg cgtggatgga ttcgcctttg ctatctacgg cgacggttac
     2461 gggcatgtct ttgacttcaa attcgtaaat ggcttccatg cccaattcgg ggaacgccaa
     2521 gactttggaa gatttgatgg cttttgccac gagatacgcc gcgccgccga ctgccatgag
     2581 gtacacggct ttgttgtcgg cgatggcttc gcaggtggcc acgccgcgct cggatttgcc
     2641 gatcatgccc aagaggtcgg tttgttcgag catttggcgg gtgaatttgt ccatgcgggt
     2701 ggctgtggtc ggacctgccg gaccgacgac ttcatcgccg accggatcga cggggccgac
     2761 gtagtaaatc aggcggttgg tgaaatcgac gggcaattct tcgcctttgt tgagcatatc
     2821 gacgaggcgt ttgtgtgcgg catcgcggcc ggtgaggatt ttgccgttca acagcaatac
     2881 gtcgccggtt ttccagcttg ccacttcttc tttggtcagc ttgtcgacat cgacgcgttt
     2941 gccgttgtcg gggctgtaag tcaaatcggg ccagtcttcg acgcgcggcg gcgtgagttc
     3001 gacagggcct gagccgtcca attcaaattc gacgtggcgg gtggcggcgc agttcggaat
     3061 catggcaatc ggtttggagg cggcgtgggt cggataatcg aggattttca cgtccaacac
     3121 ggtggtcagt ccgcccaagc cttgtgcgcc gatgcccagc gcgttgactt tttcaaagag
     3181 ttcgaggcgc agggcttcgg tggtggacaa ttccgcgccg gacgcggcct tttcctgcaa
     3241 ttcttgaatg tcgatgtggc tcatcaggga ctcttttgcc atcagcacgg ctttttcggg
     3301 cgtgccgccg atgccgatac ccaagatgcc gggaggacac cagcccgcgc ccatggtcgg
     3361 gatggttttc aatacccaat cgacgatgtt gtcggaagga ttgagcatgg cgagtttgga
     3421 tttgttttca gagccgccgc cttttgccgc gcaggttact tcgactttac cgcccggcac
     3481 gatgctcata tggatgacgg cgggggtgtt gtctttggtg ttttggcgtt tgccggccgg
     3541 atcggcgagg acggaagcgc gcagcgtatt gccttcccaa gtgtaggcgc ggcgtacgcc
     3601 ttcgttaacc atctcttcca cgctcatgtc cgcatcccat tggacgttca taccgacttt
     3661 gaggaagacg gttgcgatac ctgtgtcttg gcagatgggg cggttgtttt ccgcacacat
     3721 acggctgttg accaaaatct gcgtcatcgc gtctttggcg gcaggatttt cttccttctg
     3781 ccacgcctta taaagcgcgt cgatgtagtc tttgggatga tagtagctga tgaattggaa
     3841 ggcatcgcaa atgctttgga taaagtcttc ctgtttgatg acggtcatga ttgtgttcct
     3901 tttcaaggtt gtggcggtaa gtgtctgctg ctgttttaaa caaccttttt gaaggctgtt
     3961 ttttatcagg ttgaaatagt gccagcgttt gtacggtaaa gacaatacat aattctaatc
     4021 tcttgattat caatttgttt taataaaata ctgttttact cggattttga tatttgtaat
     4081 ctttgcctgt tgctttgcgg tagggagaaa tcagcaggca gttcattgta ggccgattga
     4141 tgaacaatgc cgtctgaaag ggcttcagac ggcattgtgt tcggacgggc gggcttattt
     4201 tgcggcctgt ccgtcttctt ttgcaaaata tttggcgtaa atcttgtcgt attcgccgct
     4261 ttcgcgtact ttttccaacg catcgttcag cattttgacg gttgcttcgt cgcctttgcg
     4321 taccgcgatg ccgtagtgtt cggtggtgaa gtcgggcagg gtaacgaagt ccatcccttt
     4381 ggccggattg tttttcacat aattggcgat gaccgcgctg tcgctgacca cggaatccaa
     4441 gccgccgttt tccagttctt tgataatcag gggaacgttt tcaaagcgcg cgattttcgg
     4501 attgtcgttg cccaagagtt tggatacgga gaaatcgccc gtgtagccgg ttaccacgcc
     4561 gactttgttc atgtttttca aatcttcgga agaagatact tttttgcctt tcggaacgag
     4621 gacgacttgg gtgatttcaa aatacgggtc gctgaagtcc atagactgtt tgcggtcgtc
     4681 ggtaatggtt acgcccgaca tcacaacgtc cgcatcgccg ttgtttaagg cggggaaaag
     4741 gctgtcccac ggctggtgtt tgaattcgat tttaaaattg cccgccttcg ccatcgcgtt
     4801 catcaaatcc acatcgaaac cttcgacatt gcctttcgag tctaaagatt caaagggggc
     4861 aaactcggcg ttggaagcca cgcggtacac tttgtcgggg ttggcggcag gcgcggcggt
     4921 atctttgccc tgaccgccgc aggcagacag cgcgagcgcg gaacaggcaa gggcggcggc
     4981 aatccatttt ttcatattca tcaaaattcc tttcggtaat caaaaagcct aaaaaatcag
     5041 tttaacataa aattaacgtt ctttttcacg gaaaaacgca taagtaacgt cgtggaacaa
     5101 atccgtatgc gggctgtcct catagctcaa ttctgccaag gcgtcctcaa aagcaagctg
     5161 aatcgactcg accgcgattt cggcggtttc ggcgggaagg gcgcggatta aggctttgaa
     5221 tgcggtggac aggacgtggt tctgcatacg cagcacgtcg ttcgcctctt ccagatattc
     5281 caaacgctgt tcgatgctgc ccatgatgta tcctcgcgaa tctgttaaaa tgcgccatta
     5341 tatcaaaccg cgctttgggc gcgggtttgt ctgcttgcaa agattaagaa ccggaagccg
     5401 aaaatggatg cgaataaaga gccgaattgg gaaaaacgtt tggaaaacct gcgtcagacg
     5461 cgccgtatga aggcagtgcc gacttacgcg ccgctgtccc gcccctctga aaccggaacg
     5521 gacgcgcctg tgccgaaact ggacgatgag agccgagccc gtgtgttgaa ggcgtatctg
     5581 aaaaaatggc aggaggaaca caatgccgcc gttgccgatt gcggcgagga agtcgaaacc
     5641 gacccgatga tcgtccttca ggaaaactgg ctcaacgcgc aggcttccct gcaaatgtcg
     5701 gtttcggaaa aacacatcga atcgcggcgg agaatccggt ttgataccaa aaaagacagt
     5761 gtcgaatttg aaaccggaca gattgaaagc ggggaaaacg ccgacgaggc agaatccggc
     5821 acgaatgagc cggcggtttc ggaaaacggg gaaaacgatg cggaagaaac gttgatgccc
     5881 gtccaaatca atatcctgaa cccgaaagcc gtcaaccggc gcgaagtgtt ctgcctgtcc
     5941 gaacaggagc tgaccgaacg gctgatcaaa cgcctgcgcc cgcatctgac cgataccgtc
     6001 aacggcatca tccgaacggc ggtgcaaaag cagatggcgg ttttcaccta tcagctccag
     6061 cagatgctgc acgagcaggc gggggcggta gtcgaagacg tgttggaaca cgatgtccgc
     6121 aaaatcctca acgacatcaa gtacgagtta aaatacaaac gctgattgcc atgccgtctg
     6181 aaagccgttt cagacggcat ttgcattctc atccgacttg aaagcatcat agaacaggcg
     6241 caaaatatta acaaattaaa caacgggcgc acgctccgtt gccgaacgga caaaaccgag
     6301 gaatcaccga tgagcaaaaa actccatccg caaacgctcg ccatacgcgg cggcaaagaa
     6361 caaaccgaat accgcgaaca caatcaggca ttgtttctga ccagcagctt catgtgggac
     6421 aacgcccaac acgctgccga tttgttttca aaaaaaatca aagggttcac ttatacccgt
     6481 accgccaacc cgaccacagc cgcctttgaa aaacgcatcg ccgctttgga aggtgcggaa
     6541 cgcgcggtcg ccacttcggc gggtatgtct gcgattcagg cggcgttttt cacctttttg
     6601 caggcgggcg accatgtgat ttccagccgc agcctgttcg gcacgaccgt cggctttatc
     6661 aataacatcg ttgccaaatt cggcatcgaa gtgagccacg tgtcgccgac tgatataaac
     6721 gaatggaaag ccgccgtcaa agccaatacc aaactgctgt ttttggaaac gccgtccaac
     6781 cccttaggcg aagtggccga cttggaagcc ttggcggaat tggcgcacgg catcggcgcg
     6841 ctcttggtgg tggacaacag cctgctgtcg cccgtcggct cgcagccttt gaaacacggt
     6901 gcggatattt ccgtttcctc cgcgaccaaa gccatagacg gacacgggcg tgtgatgggc
     6961 ggcgtgttgg cgggttcgga agagttgttg gcgcaggtcg ccatgtattg caactcttgc
     7021 gggctggcga tgtcgccgtt taatgcgtgg cagttgttga gcggcgtgga aaccctgtcg
     7081 ctgcgtatgg aaaaacagtt cgacaacgcc ctgaaaatcg cacaatggct gcaagcgcag
     7141 ccgcaggttc aagccgtgta ctacaccgga ttgtccgacc atcctcaggc ggcgcttatc
     7201 cgcaaacagc aaaacggcgg cggcatcgtc atcggctttg aagttgccga tcaagaagcg
     7261 gcgtggaaag ttgtggacgg cgtggaactc ttttcccgca ccgccaacct cggtgacgtg
     7321 cgctcgacca ttacccaccc gtggacaacc acgcacggca ggatgcagcc cgaagaaaaa
     7381 ctcgccgcca acatccgccc cggattggtg cgcctgtcgg tcggtttgga atatgttggc
     7441 gatttgattg acgatttgaa acaggcactc gcacgctgat tgcgtgtttc agacggcatt
     7501 ggacagcaga tgccgtctga aacccttatc ttatatggca tacacatcaa caataggaaa
     7561 aaaatatgga tgaagcggtt tttgccgatt gggcgttgaa aatctgcctg accggcttga
     7621 ttattttttt gggttttatc gtttggaatt tgggcaagga gtccaaagca ggcaaattcg
     7681 gcatcgccat tttgtttttg gttttgggct tgggcgtgtt cggttttgta ttcaaagaac
     7741 tgctgattaa gtttttggta ttgccgaaat aggctgccgc ctgtcaaaat gctgtctgaa
     7801 atcataaaaa gtaatctgca ccctaaaagt ttgatttaac cgccaactga ttaaggtgcg
     7861 gatttttatg acaa
//
LOCUS       DAWIVB010000094         7866 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWIVB010000094 DAWIVB010000000
VERSION     DAWIVB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.094"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            88..1317
                     /gene="sstT"
                     /locus_tag="WIX60_001357"
     CDS             88..1317
                     /gene="sstT"
                     /locus_tag="WIX60_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ5305621.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPFPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATINMAGAAITITVLAMAAAHTQGITVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGIDNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            1572..3038
                     /locus_tag="WIX60_001358"
     CDS             1572..3038
                     /locus_tag="WIX60_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ5305622.1"
                     /translation="MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVSE
                     KETEAKEDAPQAGSQGQGAPSAQGSQDMAAVSEENTGNGGAVTADNPKNEDEVAQNDM
                     PQNAAGTDSSTPNHTPDPNMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSA
                     GGQNAGNTAAQGANQAGNNQAAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQ
                     NITLTHCKGDSCSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQ
                     MKGINQYIIFYKPKPTSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGH
                     SGNIFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENG
                     RPYPTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGSGDV
                     SGKFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(3114..3329)
                     /locus_tag="WIX60_001359"
     CDS             complement(3114..3329)
                     /locus_tag="WIX60_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305623.1"
                     /translation="MFKLGVYTCLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(3322..3537)
                     /locus_tag="WIX60_001360"
     CDS             complement(3322..3537)
                     /locus_tag="WIX60_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ5305624.1"
                     /translation="MKEYKVIIYQESLLSSLFFGAAKVNPIKFSEFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(3612..4955)
                     /gene="argG"
                     /locus_tag="WIX60_001361"
     CDS             complement(3612..4955)
                     /gene="argG"
                     /locus_tag="WIX60_001361"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ5305625.1"
                     /translation="MSQNHTILQSLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            5268..6068
                     /locus_tag="WIX60_001362"
     CDS             5268..6068
                     /locus_tag="WIX60_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ5305626.1"
                     /translation="MNAFNLIIIGDEILHGSSQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHSEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            6108..7094
                     /locus_tag="WIX60_001363"
     CDS             6108..7094
                     /locus_tag="WIX60_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ5305627.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            7751..7866
                     /locus_tag="WIX60_001364"
                     /pseudo
     CDS             7751..7866
                     /locus_tag="WIX60_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1890 a         1981 c         2200 g         1795 t
ORIGIN      
        1 acagccggaa aataaaaaaa aacacagaga atgagttttc ttttaaaata caacattttt
       61 taacatatca caacataagg aagaattatg gcgttcggca aatccttatt tcatgcgatc
      121 ggccgcgtca gcctggtcag gcagattgcc gccggtttgg cgttgggcat cgtaatcggt
      181 tcggtttccc cgcaactggg cttggcggca ggcttgttcg gcagcctgtt tgtcggtgcg
      241 ctcaaagcgg tcgcgccggt tttggtattt attttggtgg cggccacaat cgcgcagcac
      301 caaaaaggca acaaggcgca tatcaggccg attatcgtcc tttacctcat cggtacgttt
      361 tccgcagccc tgaccgccgt catcgccggt atggttttcc cgacgcacat tgttttggcg
      421 ggcgcgggcg atgtgtccgc cgcgccgcct tccggcattg tggaagtatt gaaatcgctg
      481 ctgatgaacc tggtcgccaa cccgattaac gcgattgcca atgccaacta tatcggcatt
      541 ttggcttggg ctttggtttt gggcgcggcg ttgcggaatc acggttcgga cgttacgcgg
      601 caggtcgttg ccgatttggc ggaagcggtt tccaccgtcg tgaaatggat tatccgtttt
      661 gcccctttgg gcattttcgg gctggtgtcg tccacaatcg cggagacggg tttcggcgcg
      721 ctggcggggt acgcgaagct gcttgcagtg ctgttgggct gtatggcgtt tatcgcgctg
      781 gcggtcaatc ccgccatcgt gtggtggaaa atccgccgca accctttccc gctggtgttt
      841 acctgcctgc gtgaaagtgg cgtttatgcc ttctttaccc gttcttccgc cgccaatatc
      901 cctgtgaaca tggcattggc gaaaaagctg ggtttgcacg aagacactta ctccatctcc
      961 attccattgg gtgcaaccat caatatggcc ggtgcggcaa tcaccattac cgttttggcg
     1021 atggctgcgg cgcacacgca aggcattacg gttgacttcg ccactgccct gctgctgagc
     1081 ctggtggcta ccgttagcgc gtgcggtgca tccggtgtgg ccggtggctc gttgctgctg
     1141 attcctttgg cgtgcagcct gttcggtatc gataacgatg tggccatgca ggtggttgcc
     1201 gtcggcttca ttatcggcgt gattcaggat tcggcggaaa ctgcgctgaa ctcttcgacc
     1261 gacgttttgt ttaccgctgc cgccgatttg ggccgacaga ggaaccgggc ggaataaacc
     1321 aagctatgcc gtctgaagcc cgtttggcgt tcagacggca tatttttaca attttatagg
     1381 catacggcgg aaacaatatt tattgaatat gtttcaacac acagggcggc acataaagca
     1441 cagccctatg tgtcgccctg atttggaaag gttgcgctct tcccaaataa agctgattcc
     1501 accgccccga aggacggatg tccaagtggc ggggtttaaa ccaaaaagga aatacgatga
     1561 aggagatgat gatgtttaaa cgcagcgtaa tcgcaatggc ttgtattttt gccctttcag
     1621 cctgcggggg cggcggtggc ggatcgcccg atgtcaagtc ggcggacacg ctgtcaaaac
     1681 ctgccgcccc tgttgtttct gaaaaagaga cagaggcaaa ggaagatgcg ccacaggcag
     1741 gttctcaagg acagggcgcg ccatccgcac aaggcagtca agatatggcg gcggtttcgg
     1801 aagaaaatac aggcaatggc ggtgcggtaa cagcggataa tcccaaaaat gaagacgagg
     1861 tggcacaaaa tgatatgccg caaaatgccg ccggtacaga tagttcgaca ccgaatcaca
     1921 ccccggatcc gaatatgctt gccggaaata tggaaaatca agcaacggat gccggggaat
     1981 cgtctcagcc ggcaaaccaa ccggatatgg caaatgcggc ggacggaatg cagggggacg
     2041 atccgtcggc aggcgggcaa aatgccggca atacggctgc ccaaggtgca aatcaagccg
     2101 gaaacaatca agccgccggt tcttcagatc ccatccccgc gtcaaaccct gcacctgcga
     2161 atggcggtag caattttgga agggttgatt tggctaatgg cgttttgatt gacgggccgt
     2221 cgcaaaatat aacgttgacc cactgtaaag gcgattcttg tagtggcaat aatttcttgg
     2281 atgaagaagt acagctaaaa tcagaatttg aaaaattaag tgatgcagac aaaataagta
     2341 attacaagaa agatgggaag aatgataaat ttgtcggttt ggttgccgat agtgtgcaga
     2401 tgaagggaat caatcaatat attatctttt ataaacctaa acccacttca tttgcgcgat
     2461 ttaggcgttc tgcacggtcg aggcggtcgc ttccggccga gatgccgctg attcccgtca
     2521 atcaggcgga tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca
     2581 atatcttcgc gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgcccg
     2641 gcggatcgta tgcccttcgt gttcaaggcg aaccggcaaa aggcgaaatg cttgcgggcg
     2701 cggccgtgta caacggcgaa gtactgcatt tccatacgga aaacggccgt ccgtacccga
     2761 ccaggggcag gtttgccgca aaagtcgatt tcggcagcaa atctgtggac ggcattatcg
     2821 acagcggcga tgatttgcat atgggtacgc aaaaattcaa agccgccatc gatggaaacg
     2881 gctttaaggg gacttggacg gaaaatggca gcggggatgt ttccggaaag ttttacggcc
     2941 cggccggcga ggaagtggcg ggaaaataca gctatcgccc gacagatgcg gaaaagggcg
     3001 gattcggcgt gtttgccggc aaaaaagagc aggattgatg ttgatgccgt ctgaagcgag
     3061 ggcgttcaga cggcattgtg atgtttgggt cgcgggtccg gttcaagtcc gcatcagttg
     3121 ataaacccgt cgtctttcat tttcttttcg gaaaaatact gaccgcatac cagtaaggcg
     3181 gcgaggatga tgacaaacag ccccgccata gtcagtgtga ttttgaagaa caattccgct
     3241 tcgagaaaag aaaaccagag ttgcacgatc agcagcagca cccagccggc aaacagtccg
     3301 agacaggtat aaacgccgag cttaaacacg atcccgctcc aaaatgacga cgtaggcctc
     3361 gcgtttgaaa aacagcagca tacggcgcaa atctttttcc atcgttacaa cccgccagcc
     3421 ttcgggggtt tgtttattga ggaactcgct gaatttgatg gggttgacct ttgccgcgcc
     3481 gaaaaacagg ctggacaaca ggctttcctg ataaatgatg actttgtatt ctttcatagg
     3541 attcctttta acataaggga tcatcttttc agacgacccc ttaagttgct aatgatgtaa
     3601 aacagcaaac cttattgctt attgcccaac tgcggcaata ccgaaccttc gcccagcgag
     3661 agcaaaccgc tttgcgagta gatacccagt ttgacgcggg tgtcggtgat gtcgaggttg
     3721 cgcatcgtga gctgtccgat gcggtcgagc ggagtgaacg cagcgtcttc gactttttcc
     3781 atactcaggc gttcaggttg gtaggtcagg ttgggcgatt cggtgttcag aattgagtag
     3841 tcgttgccgc gccgcagttc gagggtaact tcgccggtaa cggctttggc aacccagcgt
     3901 tgtgcggttt cgcgcaacat cagggcttgg ctgtcgaacc agcggccttg gtagagcagg
     3961 cggccgaggc gcaggccgtt gatgcggtat tgttcgatgg tgtcttcgtt gtggatgccg
     4021 gtgaccaaac gctcgtaggc gatgtggaac aacgccatac ccggggcttc gtagatgccg
     4081 cgcgatttgg cttcgatgat gcggttttcg atttggtcgc tcatacccaa gccgtggcgg
     4141 ccgccgatgc ggttggcttc gaggaagagt tcgacgggat cggcgtattc tttgccgttt
     4201 agtgcaaccg gcacgccttc ttcaaagcgt acgctgactt cttccgggct gacttcgacg
     4261 ttttcgtccc aaaaggcaac gcccataatg ggtttgacga ttttgatgcc cgagttcaaa
     4321 aattccaagt ctttggcttc gtgggtggca cccaacatat tggaatcggt ggagtaggct
     4381 ttttccaccg acattttgta gttgaagccg ttggcaatca gaaattcgct catttcgtga
     4441 cggccgccga gttcgtcgat aaattgctga tcgagccagg gtttgtagat tttcagcgcg
     4501 ggattggtga gcaaaccgta gcggtagaaa cgctcgatgt cgttgccttt gtaggtgctg
     4561 ccgtcgcccc aaatattcac atcgtcttct ttcattgcgg aaacaagcat agtgccggtt
     4621 acggcgcggc ccagaggcgt ggtgttgaaa taggcgatgc cgccggtgga aacgtgaaac
     4681 gcgccgcatt ggatggcggc gatgccttcg tgtgccaact gcgcgcggca gtcgattaag
     4741 cgggcgtttt ccgcaccgta ttccatcgct tttttgggaa tggcgttgta gtcgtcttcg
     4801 tcgggctggc cgaggttggc agtgtaggca taaggcagcg cgcctttgag tttcatccac
     4861 aacagcgcgg cagaggtatc aagaccgccg gagaaggcga tgccgacttt ctgaccgacg
     4921 gggagggatt gcagaatggt atggttttgg ctcataaggg tatccttcgg acttgaaaaa
     4981 aggagttcat tatcttataa atataaccca ttgtcagccg ttaaggctgt ctgaaaagaa
     5041 gctttgcccg tttcgctgaa aatcttttca gacggtcttt acgccatcct caacctgact
     5101 ccctcccctg cgggggaggg ttggggagag ggtacagaca gcaggcctta agcaaacgct
     5161 gcctgaaaaa cgcaagctcg accctctccc taaccctctc ccacgggaga gggaacaaat
     5221 cacagcacca tcgacatttt cagacggcat cggtaaagaa accatctatg aacgcgttca
     5281 acctcatcat catcggcgac gaaatcttac acggcagcag ccaagacaag catttcgcct
     5341 ttttcaaatc cctgctcgaa tccaaagggc tgaagctcaa tcaggtgcaa tacctgcccg
     5401 acgaacccga tttgctggtc aggcaactgc gccgcagctt ttcagacggc atgccaactt
     5461 tcgttaccgg cggtatcggc gccacgcccg acgaccacac ccgccaagcc gcagccgctg
     5521 ctttggattt gcccgtcgtc cgccattctg aagccgccaa gtttatcgaa ggcataaccc
     5581 agaaacgcgg cgagccgctt gattcgccgg aacacgccca acgcctgaaa atggcagatt
     5641 ttcccgaagg cgcggaattg gtgcccaatc cgtttaacaa catcgccgga ttttctatcc
     5701 gcgagcatta tttcttcccc ggctttcctg tgatggcgca cccgatggcg gaatgggtat
     5761 tggaaactta ttacgccgac cgcttcaacc aaaccgaacg cggcagccgc agcgtgtatg
     5821 tgtttgagca gcccgaatcg cgcattacgc cgcttatgga gcatatcgag caaacttatc
     5881 ccggcgtgcg ttcgtacagc ctgcccagcg tcggttggac gcattcagac ggcacgcagg
     5941 tcaaaccgca cattgagttc ggcatcaaag ccgaaggaga agccgtcaac cttttggacg
     6001 cggcgtgggc ggaagtgttg cacagcttgg acggattggg agcagagctg aaaaatcggg
     6061 taaactgaaa accgtttgcc caaatcggac aatatttaag gaaaaccatg caataccgaa
     6121 tcagaagaga aaacgaagca cccgaagcaa aaaatgcggg ggaaacctta tgggagcgcg
     6181 acatcatgcg cgaagtgctg ttgtccgcct atcaggacag gcgcagggag cggatgtgga
     6241 aaaacatctg gcgcgccgtc agcaccctga ttctggttgc cctgattgca ggcattttcc
     6301 gaaaagacga agccgcattg cagttggcgg gcaacacgcc gcataccgca gtcgtcaatc
     6361 tgtacggcga aatcggcaac ggtgtagaag accaggtcaa aaaactcaaa gacggtatgg
     6421 aggccgccta caaaaacccg caggcaaaag ccatcgtcat ccgcgccaac agccccggcg
     6481 gttcgcccgt cgtgtccaac accgcttttg aagaaatacg ccgtctgaag gcgcagcacc
     6541 ccggcattcc cgtttatctc gtggcggaag atatgtgcgc gtccggctgc tactacatcg
     6601 cggcggcggc ggacaaaatc tatgccgacc cgtccagcat cgtcggcagc atcggcgtta
     6661 tcggcagcag tttcgacgcg accggcctga tggaaaaaat cggcgtgaaa cgcagggtta
     6721 aaattgcagg cagcaacaaa ggcatgggcg atccgttttc gcccgaaacg cccgaacagt
     6781 caaaaatctg ggaagaaatg ctgaccggca tacacggcga gttcatcaaa gccgtcaaaa
     6841 ccggacgcgg cggccgcctc aaattccgac aatatcccga cgtgttcagc ggtcgcgtgt
     6901 acacaggcgc ggatgcgctg aaagtcgggc tggtggacgg actcggcaat atttacagcg
     6961 tggcgcgaga cgtggtcaaa gcccccgatg tggtggatta cactccgaag gacgatttcg
     7021 gcagaatctt gggcagacgc ttcggcgcag aactcaaagc aagcgtgcgc gaagccctgc
     7081 aggccgtccg gtagcgttgt ttacagtatt tctaagggaa taccaaaata aaaatataca
     7141 caaaaacact cacctcccca gtatgctgcc actaagtcag ccgcgtcact atttaccgtg
     7201 cacttaaagc cgtaaaaggc aggttgtccc cgggaggttt tgtgcatcct gataccgatc
     7261 ggttgttgtt gctcaaagga cagaaggccg ctgataaacg agattacctg tttgtcgcta
     7321 ttgacgattt ttatactctg ccattttgcc ggacaaaacc gcagacagtg ctgccaagtt
     7381 tctgaccgaa catctgtccg acccctgctt gtacctgatt gagtacgttt actctgacaa
     7441 tgataggtaa tataaagagc cgtccaacat gctttcggtg cagcttgtta tgataatggg
     7501 attggttgga ggcttgcccg atttgcttgt ccgcagacca acggtaaggc ggagcgggtt
     7561 atccgtacct tgatggagat gtggcatgag gaacagtcgt ttgacagtcc ggaacatcgg
     7621 caaaaggagt tgtgctgctt tattaattta ggggctgccc tagatcacta gggaaattca
     7681 aattaagtta gaattatccc tatgagagaa agtcgtctaa gacggtataa acaaaataaa
     7741 ctcattttgt tatcgtggct atgatgtatt ggatgcgggc gaatttagcc atttccgtat
     7801 caatcacagc acacattttg ccgaacgaca aaaccatatt aatggaactg agaacttttg
     7861 gaatca
//
LOCUS       DAWIVB010000095         7793 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWIVB010000095 DAWIVB010000000
VERSION     DAWIVB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.095"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            41..505
                     /locus_tag="WIX60_001365"
     CDS             41..505
                     /locus_tag="WIX60_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ5305628.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            1196..1684
                     /locus_tag="WIX60_001366"
     CDS             1196..1684
                     /locus_tag="WIX60_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ5305629.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAIVLMCRPRRKAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(1743..2471)
                     /locus_tag="WIX60_001367"
     CDS             complement(1743..2471)
                     /locus_tag="WIX60_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ5305630.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            2798..4216
                     /locus_tag="WIX60_001368"
     CDS             2798..4216
                     /locus_tag="WIX60_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ5305631.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAVRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            complement(4280..4906)
                     /locus_tag="WIX60_001369"
     CDS             complement(4280..4906)
                     /locus_tag="WIX60_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ5305632.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            5049..6398
                     /locus_tag="WIX60_001370"
     CDS             5049..6398
                     /locus_tag="WIX60_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ5305633.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRFLAWAVCKILLKTSK
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKTNDRT"
     gene            6460..>7793
                     /locus_tag="WIX60_001371"
     CDS             6460..>7793
                     /locus_tag="WIX60_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HEZ5305634.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDATRLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
BASE COUNT         1719 a         1973 c         2100 g         2001 t
ORIGIN      
        1 tctatttgtt aaaatcaata ataaatacaa ggcaatctaa atgaaagaac tagataaaat
       61 cgatttccgc atccttaaga tccttcaaca gaatgcccgc atcccgatga cggagcttgc
      121 cgagaaggta ggcttgtcca ccacgcccgt tacagagagg gtgcgccgtt tggagcggga
      181 acattatatt tccggttatc acgcccatct caatccccat ctgttgggca aacctttatt
      241 ggtttttgtc gagctgaagc tgcaatccaa atcgggcaat attttcgaag atttcaaaaa
      301 agaagtgctg aaaattccgc aaattatgga atgccacttg gtgtcgggcg aatacgacta
      361 tctgattaaa gtacgtttgc ccgatatgtc tgcctatcgg gatatgctag gcaatatcct
      421 attgcaactg cctgccgctt cggagagtag gagttatgtt gtgatggagg aggtcaaaga
      481 aaatccggtt ttggatttgg attgaatatt tcagaccgga tatgcggttt gacacctaaa
      541 tgaaacataa caacatcacg cacagaggct tgttgcttca gataaaaaaa tgcacacatc
      601 cagttgaatg tgtgccaagt ctacaaagga gaaatagagg agaaactatt aactgactac
      661 tcgaaccagc taacggaatg aattatgcag cttttccggt caaaaagcaa gcgtttttct
      721 tgttaaattt cttaaagaat ggaatttcaa acaataaaag taattaaatt tcaaaaaaag
      781 cggagataaa aaaatgcaca catccagttg aatgtgtgcc aagtctacaa aggagaaata
      841 gaggagaaaa atcaagctgc atcaagcaac tcgacggcgg gaattatccg ccgtttctta
      901 tccgctgtca attatttttt gaaaatttac ccgaccgatt caaattttgc aaaaaacttg
      961 cccgatggct atccggtaac aaattccttc aaaaacccat caatccgctt attcaaaaaa
     1021 atcatatgat ttcattgaaa taaacttttg attcgctaaa tttgctgaaa ttcaaatcct
     1081 ccggatgtat ttttttgtgg gcattgtgcg ggcaaatcgc cgttttgtcc cttgccatct
     1141 cgggatatat ccagcaaaat cggcatatcg gtattttgat tggtgtgttg agaaaatgac
     1201 cccgttattt agaaaagccg tttggctgct gtttgccgtt tcggtctgtg catttgccgg
     1261 ctctttggcg gcacagtatg ttttgggtat ggagccttgc gttttgtgta tcagccagcg
     1321 gttgtgcgtc ttggcaaccg cattgtgtgc ggcaattgtc ctgatgtgca gaccaagaag
     1381 aaaggcaggc ggtttgttcg gtgctgtctt tatcagcatt cccgccgtta cgggtatttc
     1441 tgttgcggca tatcagttgt ggttgcagtc gctgccgccg ggaaccgctc cttcgtgcgg
     1501 cgcgccgtgg acgttccgat tgaagggctg gcctttgttt gattggttcg agcctgtcgt
     1561 gcgcgggttc ggaaattgtg ccgaaccgga ttatctgctg ggggttgctt tgcctgtttg
     1621 gagtgtggcg tattttctgg cggttgtcct gacggtttgg tgggcgtggg caagggctaa
     1681 ataaatcaat gccgtctgaa aggttcagac ggcattttat tgtatgtctg ctgtgctgcg
     1741 tatcagtcca gattcaatac ggcggaagtg taaacgtctt gcacgtcgtc caaatcttcc
     1801 agcgcgtcaa tcagtttttg cattttgacg gcatcgtcgc cggagagttc ggtttcgttt
     1861 tgggcgcgca tcgtaacgtc gccgtcaacg gatttgtaac ctgccgcctc caaagcggat
     1921 tttacgcccg cccaatcgtt tggcgcggta atgacttcga tggaaccgtc gtcgttggta
     1981 accacgtctt ccgcaccggc ttccaaagcc gcttccatca gcgcgtcttc gtcaacgccc
     2041 ggttcaaaca ccaaatagcc ttgatgaacg aagttgaacg ccacgcagcc gtcggtaccc
     2101 aagttgccgc cgtttttggt aaacgcgtgg cgtacgtccg caacggtgcg ggttttgttg
     2161 tcggtcaggc agtccaccat caaagccgcg ccgccgatgc cgtagccttc gtagcgcaac
     2221 tcgatgtatt ccacgccttc caagttgccc gtacctttgt cgatggcgcg ttgcacattg
     2281 tctttgggca tattgttttc ggctgctttt tccaaagcca ggcgcaggcg cggatttgaa
     2341 ccggggtcgc cgccgcccat acgcgccgca acggtgattt ctttgattaa acgggtgaag
     2401 attttgccgc gtttggcatc ctgacgggct tttttatgct ggatattcgc ccacttgcta
     2461 tggcctgcca taaatttcct ttcttatttc aaataattaa tctgtttttg tcagattttt
     2521 tacggtcggc attttaacat aagttgcaag tacccaaaat atcctgtttt atgccgtgcc
     2581 gttgtcgatg ccgtctgaaa gcgatttcct ccgctttccg cttaaaaata aagaaatgtt
     2641 ttaattaacg atattttacg gtatgatggc gcagcttctc cccgattgtt gacaatttaa
     2701 ggcatctgtt gcggcaaata cggtctgctc gccgtgtttc cttattggaa tgtcttttgg
     2761 aaggcagtca ttttaattgt aagataaggt ttattttatg gaaaatatat tgtctgttct
     2821 ggtgggtaca gtcaatcggt tcctttggga ctacctgctg atttacgcgc ttttgggtat
     2881 cggcctgttt tttacgctgt atctcggtgc gccgcagatt accaagctgg gcgcgggatt
     2941 caaatccgta ttcggcggct tgtttgccaa aggcgataaa gacgacaagt ctttatcgca
     3001 gtttcaggcg ttggcggttg ccatatctgc gcaaatcggt acgggcaacg tcgccggcgt
     3061 ggcgaccgcc atcaccgcag gcgggccggg cgcgattttt tggatgtggc tttctgccgt
     3121 tttggggatg tccacgattt ttgccgaagc gctgctggcg cagaaatacc gcgtcgtcag
     3181 ccacggcaaa tacatcggcg ggccggcgtt ttatattacg cacggtctga ctccgaaaat
     3241 cggcaggggc gcggcgcgtt ttctgtcggg ctttttctct atcgcgctga ttgtcgcatt
     3301 gggctttatc ggcaatgcga ctcaggcaaa ctccatcgct tctgccgtta ccattgcatt
     3361 tgatgtacct tctttggcag tcggtattgt gcttgccgtc cttgcgggca tggttgtgat
     3421 tggcggcgtg aaccgtattg ccaatattgc ccaatttgtc gtgccgttta tggcggttgt
     3481 ttatattttg tgcgccgtcg ttatcctgtt tgaattttcc gaccatattg tgccgatgtt
     3541 caaccacatc tttaccgccg ccttcaatcc cgaagccgtt ttgggcggtg cggcgggtat
     3601 cggtatgcgc gaagccgtcc gtttcggcgt ggcgcgcggt ctgttttcca acgaagcggg
     3661 tatgggttct accccgcacg cccacgcgac tgcggatgtg aagcatccgg tgcagcaagg
     3721 tatggcggca tttgtcggtg tgtttatcga tacgattttg gtatgtacgg caactgcgct
     3781 gattatcctg ctgaccgatg ccaacctttc gggcgaacag ggcgcggcgg ttactcaatt
     3841 tgcctttaac aaggcatttc cgggcttcgg ttcgcaattg cttgccgtgt gtctgacctt
     3901 tttcgccttg acgaccatta tcggctggta ttatttcggc gagtccaaca tccgttttct
     3961 tttcagggga agacacttgg gcatctatcg cgcattggtt ctgcttgcca tcgttttggg
     4021 tacgctcggc aaagtcgatt tggtttggag cctgtccgat atgttcaacg gctttatggt
     4081 tatcccgaac ttgatagccc tgtttttgtt gcgtaaggaa atccgtgcca tttatgatga
     4141 ttatttggca caaaaaaaag cgggtcggga tttgtcttat ccgtatgaat tccacgagtt
     4201 ccacgacaag ggctgatgtc cggcaaacag aaaacctccc aatcttcagg aacgggaggt
     4261 tttgcttttc agacggcatt tagattacgt aaccgtacac gctgacaaat tgggtgatgc
     4321 tgccgcccaa tacgaacaga tgccagattc cgtgcccgtg tcggattttt tcatcgttta
     4381 caaaccagta aatgccgacg ctgtacagca taccgcctgc cgccagccaa gccagtcctg
     4441 ccggcgggag tgaggctgtc agggatttca ttaccgccaa gaccatccag cccattacga
     4501 tataaatcgc aatagacagc agtcgttttt cgcttttccg tccaatggtg agttcttgtg
     4561 cgattcctgc agccgccagc agccaggaca gtgaaaatac cgtccagccc ggcccgtttc
     4621 tcaaagaaac cagtgcaaac ggtgtgtagc ttccggcaat cagcacataa atcatgcagt
     4681 ggtcggtttt tttcaaaatg cttttcagtt ttccggctgc aattccgtgg tacagcgagg
     4741 aactcaaata gagcagaaga aggctgatgc cgtaaaccga tacgctgaag atacggtagc
     4801 cgtccccgtg tcctatggtt ttcagcagca tcagcgccaa acctgccgcc gccagaatca
     4861 aaccgctcaa atggctgtaa gtattgaagc gttcgcctgt atacatcgca caatctttcg
     4921 ggaaagtaga ggcagtcagt ttacgccgat acttttgcct ctgtgtgaga tttactcttt
     4981 gcctacactg tgaatattct tgaaaatgcg tgtgttaaaa tctgtttttt attttcagac
     5041 ggcattttat gttgaatcca tcccgaaaac tggttgagct ggtccgtatt ttggacgaag
     5101 gcggttttat tttcagcggc gatcccgtac aggcgacgga ggctttgcgc cgcgtggacg
     5161 gcagtacgga ggaaaaaatc atccgtcggg cggagatgat tgacaggaac cgtatgctgc
     5221 gggagacgtt ggaacgtgtg cgtgcggggt cgttctggtt gtgggtggcg gcggcgacgt
     5281 ttgcgttttt taccggtttt tcaggaactt atcttctaat ggacaatcag ggtctgaatt
     5341 tctttttggt tttggcgggc gtgttgggca tgaatacgct gatgctggca gtatggttgg
     5401 caatgttgtt cctgcgcgtg aaagtggggc gttttttcag cagtccggcg acgtggtttc
     5461 ggggcaaaga ccctgtaaat caggcggtgt tgcggctgta tgcggacgag tggcggcaac
     5521 cttcggtacg ttggaaaata ggcgcaacgt cgcacagcct gtggctctgc acgctgctcg
     5581 gaatgctggt gtcggtattg ctgctgcttt tggtgcggca atatacgttc aactgggaaa
     5641 gcacgctgtt gggcgattcg tcttcggtac ggctggtgga aatgttggca tggctgcctg
     5701 cgaaactggg ttttcccgtg cctgatgcgc gggcggtcat cgaaggtcgt ctgaacggca
     5761 atattgccga tgcgcgggct tggtcggggc tgctggtcgg cagtatcgcc tgctacggca
     5821 tcctgccgcg cttcttggct tgggcggtat gcaaaatcct tttgaaaaca agcaaaaacg
     5881 gcttggattt ggaaaaaccc tattatcagg cggtcatccg ccgttggcag aacaaaatca
     5941 ccgatgcgga tacgcgtcgg gaaaccgtgt ccgccgtttc gccgaaaatc gtcttgaacg
     6001 atgcgccgaa atgggcggtc atgctggaga ccgaatggca ggacggcgaa tggttcgagg
     6061 gcaggctggc gcaggaatgg ctggataagg gcgttgccac caatcgggaa caggttgccg
     6121 cgctggagac agagctgaag cagaaaccgg cgcaactgct tatcggcgtg cgcgcccaaa
     6181 ctgtgcccga ccgcggcgtg ttgcggcaga tcgtccgact ttcggaagcg gcgcagggcg
     6241 gcgcggtggt gcagcttttg gcggaacagg ggctttcaga cgacctttcg gaaaagctgg
     6301 aacattggcg taacgcgctg gccgaatgcg gcgcggcgtg gctggaaccc gacagagcgg
     6361 cgcaggaagg ccgtctgaaa accaacgacc gcacttgaaa cgccccataa agccgccatc
     6421 cttaaaaggt cgtctgaaaa tccattcttc aaaaacatca tgaacaaaca acccctttcc
     6481 ctcgccgtcg tcggacacac caacaccggc aaaacctcgc tcctgcgtac ccttttgcgc
     6541 gacagcggtt tcggcgaagt cagaaacgcc ccatcgacca cgcgtcatgt cgaagaagcc
     6601 gccatcagcg acggcgcaga cacgctggtt ttcctgtacg acacgcccgg actcgaagac
     6661 gcgggcggcg tgctggaatg gctggaaaac catacggaca atcgttcaga cggcatcgaa
     6721 cggctgcaac agtttctcgg cagccacggc gcgcaccatg atttcaatca ggaagccaaa
     6781 gtcttacggc aagtcttgca aagcgatatg gcaatgtacg tcatcgacgc gcgcgaaccc
     6841 gttctcgaca aatatcggga tgaattgatt atcctttcat ggtgcgccaa accggttatg
     6901 cccgtgttca actttaccgg cggacagcct cccgaatcgt ggacaaccat gctggcgagg
     6961 agaaacctgc acgttttcgc agggttcgac accgtcgcct ttgattttga aggcgaactg
     7021 cgcctgtggg aaaacctcgc caccatgttg cccgaacgca gcacactcga ccgcctgaca
     7081 gccatgcgcc ggcgcgaatg gcagcggctg gacggcgaag cgcgccgcga aatcgccgat
     7141 ttcctgctcg atgccgccgc cttcaggcag gaagtggacg aaaacgagga taccgccacc
     7201 gtgctgcaaa ccatgcaggc ggaaatacgc caactcgaac ggcagatgca gcagcggctg
     7261 tttgccctct accgtttcta ccacagcgaa atcgacggcg gcgactggat gccgcaagcc
     7321 ttccgccaag acccgttcga cagcgaattg ctcaaacaat acggcatccg caccggcacg
     7381 ggcgcggcaa ccggcgcgct catcggcttg gggctggaca tcgccacact cggcggctcg
     7441 ctcggattgg gtacggcaat cggcggcttt ttgggcggca tcctgcccaa tacccgcacc
     7501 atttccgaca aactctccgg ccgccaaacc ttgcacaccg accctgaaac cttgaccctg
     7561 ctcgccgccc gcgccctcga tttgctccac gtcctgcaaa cgcgcggaca cgcggcacag
     7621 tcggatatcg agctgcacag ccgcaaagcc ccgtgggacg ccaccagact cccgcccgaa
     7681 ctcaacaaag cccgcagtca ttggaaatgg tcgtcgctca atacgcaccg ccccgaaacc
     7741 agccgcgccg aacgcgccgg atatgtggag aaacttcaaa tccgcctgtc ggg
//
LOCUS       DAWIVB010000096         7660 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWIVB010000096 DAWIVB010000000
VERSION     DAWIVB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.096"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            318..452
                     /locus_tag="WIX60_001372"
                     /pseudo
     CDS             318..452
                     /locus_tag="WIX60_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            407..622
                     /locus_tag="WIX60_001373"
     CDS             407..622
                     /locus_tag="WIX60_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ5305635.1"
                     /translation="MFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPIA
                     KIWKLVGWMGCSARSASWLITWYSAAG"
     gene            825..1633
                     /locus_tag="WIX60_001374"
                     /pseudo
     CDS             825..1633
                     /locus_tag="WIX60_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            1877..2533
                     /locus_tag="WIX60_001375"
     CDS             1877..2533
                     /locus_tag="WIX60_001375"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ5305636.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            2689..4374
                     /gene="rpsA"
                     /locus_tag="WIX60_001376"
     CDS             2689..4374
                     /gene="rpsA"
                     /locus_tag="WIX60_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ5305637.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            4385..4699
                     /locus_tag="WIX60_001377"
     CDS             4385..4699
                     /locus_tag="WIX60_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ5305638.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(5050..5457)
                     /locus_tag="WIX60_001378"
     CDS             complement(5050..5457)
                     /locus_tag="WIX60_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ5305639.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            5605..6714
                     /locus_tag="WIX60_001379"
     CDS             5605..6714
                     /locus_tag="WIX60_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ5305640.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            6723..7550
                     /gene="fghA"
                     /locus_tag="WIX60_001380"
     CDS             6723..7550
                     /gene="fghA"
                     /locus_tag="WIX60_001380"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ5305641.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         2043 a         1866 c         1837 g         1914 t
ORIGIN      
        1 aattccctct ctttcatcat tatttaaatg ctatcattaa tataattaac aaaatttcat
       61 ttccatacac cgatgtagaa gaataacaaa ttaatctatt tataaactcc gattcttcct
      121 catcgggtta tactcatcag aaatactcac tgaaatatat gccttttaca tggaaaatca
      181 ctagacagtt gaaaagcaat cagtcagttc ctgttaaaaa acgcgtgtag aatttccgct
      241 ttatctcaat cggacacggt ttcaatatgt cttccccttc aaataccaat cgtcaaacct
      301 ggtccagccg attaacctat atcctgaccg ttgccggcgc gactgtcggt ttcggcgcga
      361 cgtggcgttt cccgtatttg gtcggtgaaa acggtggtgc gcgtatgtgt ttttattctg
      421 tatcgcgatg ctggttatcg gcatcccgat gattttggtg gaaaatgtca tcggacggcg
      481 caaaggcgtg aacgcgctgg atgcgttcgg cggcccgatg aacggcaaac ccattgccaa
      541 aatttggaaa ctggtcggct ggatgggctg ctcggcgcgt tcggcatcat ggcttattac
      601 atggtactcg gcggctgggt aatcagctat atcgttaata ttattggagg aaatttgaat
      661 atttccagcc ccgtcgacgg tgtggttaca aaaggcttct ttgccgaaca cattgaaaca
      721 gcccttggga aattgcgttt tatacgctgc tttttgtcgc cgtgaaccaa tggattttgg
      781 tcaaaggcgt tatcggcggc attgaaaaag cggcaaaata gctgatgccg ctgctgtttt
      841 tgttcctaat cgcgatggtc gtccgcaacg ttacccttcc gggcgcaatg gaaggggttg
      901 ctttctatct gaaacctgat ttcagcaaga ttaccgccga actgttcgtc ttcgttttgg
      961 ggcaggtatt ttttgccctg agcttgggtt tcggcgtgat gattaccttg tccagctatt
     1021 tggataaaaa cgaaaatctg gttcagacgg cagttatcac ggcaattacc aataccatca
     1081 tcgccatact tgcgggcttt atgattttcc cgtcgctctt cagcttcggc gttgcccccg
     1141 attccggccc gactttggtg ttccaaagct tgccgattgt gttctcacat atgtgggcgg
     1201 gatctgtgtt cgccgtgatt ttcttctcgc tgctcctgat tgccgcgttg acaacttcgc
     1261 tgaccattta tgaagtgttg attacgacca ttcaggaaaa aaccaaaatc cgccgtaccg
     1321 ccgcgattac gattgtattg gctgccatct tcattttcgg caacatcccg tccattctga
     1381 gctatggtcc gtggaaagac gttccgtgtt cggcaaaaat attttcgatg ccttcgacta
     1441 catcagcggc aacatcttgt ttatgctgac cgcgctcggt tccgcgctgt ttgccggttt
     1501 tgtgatgaag gacgaagcga aggacgaatt gctttataaa ggcaaccata cgacggtcaa
     1561 tatttggttt gcttatgtga aatatcttgt gccgctggtg attctgctga ttttcgtcag
     1621 caacctgttc taatccgcag caatcgatgc cgtctgaagg tcatacctct ttcagacagc
     1681 attgccttga tgcccgccac aatacccgca acgccgaaat ccgaaccggc ttgccggctt
     1741 ctgtcggatg tttccgggca ggcggcgttc cgtctgctta gacaatcgtc ctttaaaaca
     1801 ggtagaatcc gccccaacgg gaaacacatc cttcagacgg caaaacccat accccaaacc
     1861 atcaggaatc ccccttatga acagacaaaa agtcatcgcc atcgacggcc cgggcgcatc
     1921 gggcaaaggc acggttgccg cccgcgttgc cgccgcattg ggatacgatt atctcgatac
     1981 cggcgcactc taccgcctga ctgccctata tgcacaaaaa caaggcgtgg gatggcacga
     2041 tgaagaaaac gtttccgaac tggcaaaaaa actgcccgcc gtattttcag gcagccgcat
     2101 cctgctcggc ggcgaagacg tttcagacgg catccggaca gaagccatcg gcatgggcgc
     2161 atccgcagtc gcacagttgc ctaaagtccg cgccgccctg ctgcaacgcc aacgcgattt
     2221 tctgaccgaa aaaggactgg ttgccgacgg acgggacacc ggatcggtcg tcttccccca
     2281 agccgaactt aaaatcttcc tgacggcaga atccaaaatc cgtgccgaac gccgcgccaa
     2341 acaaatcggc atcccctgcg aaggtttggc attcgagcgc atcctgtccg acatcgaagc
     2401 cagagacgag gcagaccgaa accgcaaagt tgcccccctg aaacaacagc ccgatgccct
     2461 gcttttggac acaagccgcc tgactataga agaaactgta aaaaaagtgc ttgattggta
     2521 tcgtgaagtt taaattttca ggtataatcg cacaaattac gtttcagacg gcataaaaat
     2581 cccccatatg ccgtctgaaa ccttatgtac ccgtctgccc tgccaaggac ggcagatatc
     2641 caccaaccca ccccgcaccc cttggcggtg taccgaaaag agttatatat gtctatggaa
     2701 aattttgctc agctgttgga agaaagcttt accctgcaag aaatgaaccc gggtgaggtg
     2761 attaccgctg aagtagtggc aatcgaccaa aacttcgtta ccgtaaacgc aggtctgaaa
     2821 tcagaatccc tgattgatgt agctgaattc aaaaacgctc aaggcgaaat tgaagttaaa
     2881 gtcggcgact tcgttaccgt taccatcgaa tccgtcgaaa acggcttcgg cgaaaccaaa
     2941 ctgtcccgcg aaaaagccaa acgtgcagcc gattggattg ccctggaaga agccatggaa
     3001 aacggcgaca tcctgtccgg catcatcaac ggaaaagtca aaggcggcct gaccgttatg
     3061 attagcagca tccgcgcatt cctgccgggt tctttggtcg acgtacgtcc tgtaaaagac
     3121 acttctcact tcgaaggcaa agagatcgaa ttcaaagtga tcaaactgga caaaaaacgc
     3181 aacaacgtcg ttgtttcccg ccgcgccgtt ctggaagcca ctttgggtga agaacgcaaa
     3241 gccctgctgg aaaacctgca agaaggctcc gtcatcaaag gcatcgttaa aaacattacc
     3301 gattacggtg cattcgttga cttgggcggc atcgacggtc tgttgcacat caccgatttg
     3361 gcatggcggc gcgtgaaaca cccgagtgaa gtcttggaag tcggtcagga agttgaagcc
     3421 aaagtattga aattcgacca agaaaaacaa cgcgtttcct tgggtatgaa acaactgggc
     3481 gaagatcctt ggagcggtct gacccgccgt tatcctcaag gcacccgcct gttcggcaaa
     3541 gtatccaacc tgaccgacta cggcgcattc gtcgaaatcg aacaaggcat cgaaggtttg
     3601 gtacacgtct ccgaaatgga ctggaccaac aaaaacgtac acccgagcaa agtcgtacaa
     3661 ctgggcgacg aagtcgaagt catgattttg gaaatcgacg aaggccgccg ccgtatctct
     3721 ttgggtatga aacaatgcca agccaatcct tgggaagaat ttgccgccaa ccacaacaaa
     3781 ggcgacaaaa tctccggcgc ggttaaatcc attaccgatt tcggcgtatt cgtcggcctg
     3841 cccggcggca tcgacggttt ggttcacctg tccgacctgt cctggaccga atccggcgaa
     3901 gaagccgtac gcaaatacaa aaaaggcgaa gaagtcgaag ccgtcgtatt ggcaatcgac
     3961 gtggaaaaag aacgcatctc cttgggtatc aaacaactgg aaggcgatcc gttcggcaac
     4021 ttcatcagcg tgaacgacaa aggttctttg gttaaaggtt ccgtgaaatc tgttgacgcc
     4081 aaaggtgctg ttatcgccct gtctgacgaa gtagaaggct acctgcctgc ttccgaattt
     4141 gcagccgacc gcgttgaaga tttgaccacc aaactgaaag aaggcgacga agttgaagcc
     4201 gtcatcgtta ccgttgaccg caaaaaccgc agcatcaaac tttccgttaa agccaaagat
     4261 gccaaagaaa gccgcgaagc actgaactcc gtcaatgccg ccgccaatgc gaatgccggc
     4321 accaccagct tgggcgacct gctgaaagcc aaactctccg gcgaacaaga ataaggttgc
     4381 agacatgaca aagtctgagt taatggttcg tttggcagaa gtgtttgccg ccaaaaacgg
     4441 cacgcatctt ctggcaaaag acgtagagta cagcgtaaaa gtcttggttg acaccatgac
     4501 tagatcgctt gcccgaggtc aacgcatcga aatccgcggt ttcggcagct tcgatttgaa
     4561 ccatcgtcct gcccgcatcg gtcgcaatcc caaaaccggc gagcgtgtgg aagtacctga
     4621 aaaacatgta ccccacttca agcccggtaa agaattgcgc gagcgggtcg acttggcttt
     4681 aaaagaaaat gccaattaaa ccttagcatc aaaacgccgc tgttacgcgg cgttttttct
     4741 gtggtttaac ttcatccgtt gcttcaatac cttgagccaa gcaagcaacg gattagagcg
     4801 tggatttttt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa
     4861 gagaacaatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atttacactg
     4921 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatcatt gcttacaatc
     4981 cgctttttaa acaacaaatt tttgatttct attacgaaca ggacaaaaat cctgcttatt
     5041 gcactaaaac taagccgttt caggaatttg cggcaaattt acagctttta ccgagcctaa
     5101 tgctttcgct ttttggtaaa acgccaattt gtattcaagc aaatctaaat agcgttttaa
     5161 ttcggcaatt tgacacttca cattttctat ttgattttca aacaaggaaa ggcgttcttc
     5221 aatggtatcg tcgccaatga cggtacattc cgcaaagcgt ttgatgtctt ttaagctcat
     5281 tcccgtattt ttcaagcatt gcaataagcc caaccattgc aaatcgttat cggtaaaaca
     5341 gcggttaccg tattcatcac gtccgatatt gggcaacaaa ccttctttgt cgtaaaaacg
     5401 tagggtgtgg gcggagatgc ctattttttc ggcggctttg gcagtagtat aagtcatttt
     5461 ccctccttct aaacaaaaac agtaaaaaac acttgcttta gagttaactc taaagtgtaa
     5521 actgttgcta tgttgctcag gcaaggcaac tttgtcaatg aattaagagg aaagacaatg
     5581 gaaatgaaac aagccgattc aaccatcaaa tctcgtgcgg cggtggcatt cgcccctaac
     5641 caacccttac aaattgtgga aatcgacgta gaaatgccgc gtaaaggcga ggtgttaatc
     5701 cgcaataccc acactggcgt gtgccatact gatgcgttta cgttatcagg aagcgatcct
     5761 gaaggcgtat tccctgtggt gcttggacac gaaggtgcgg gtgtggtcgt tgctgtgggc
     5821 gaaggtgtgt caagcgtaaa accgggtgat cacgtgattc cgctttacac cgccgaatgt
     5881 ggcgaatgtg agttttgttg ttcaggtaaa accaacttgt gcgtctcagt gcgtgataca
     5941 caaggtaaag gcttaatgcc ggacggcacg acgcgttttt cttatcaagg tcagccaatc
     6001 tatcactata tgggctgttc gactttcagt gaatactccg ttgttgccga agtttcactg
     6061 gcgaaaatca accctgaagc caaccatgaa caagtatgtt tgctcggctg cggcgttacc
     6121 acaggtattg gtgcggtaca taatacggca aaagtgcaag aaggcgactc tgttgccgtg
     6181 tttggtttgg gggcgattgg tttggcggtg gtgcaaggtg cgcgtcaagc caaagccggc
     6241 cgcattatcg ccattgatac caatccatca aaatttgagt tggcaaaaca gttcggtgca
     6301 acggattgtt tgaacccgaa cgattacgat aaaccgatca aagatgtgtt gttagacatt
     6361 aataaatggg gcattgacca tacctttgaa tgtatcggca atgtaaacgt aatgcgtcag
     6421 gcattagaaa gtgcacatcg tggttggggt caatccatta tcatcggcgt agcaggtgca
     6481 ggacaagaaa tttcaacgcg tccgttccag ttggtaacag gtcgtgtttg gaaaggttca
     6541 gcatttggcg gtgtgaaagg tcgctctgaa ctgccgaaaa tggtggaaga ttcaatgaaa
     6601 ggcgacattg agttagaacc gtttgtaacc cacacaatga cactcgatca aatcaataaa
     6661 gcctttgact taatgcacga aggtaaatcg atccgcgccg ttattcacta ctaaggtatg
     6721 cgatgaaact gattgaacaa catcaaattt ttggtggttc gcaacaagtt tgggcgcatc
     6781 atgcccaaac gctgcaatgc gaaatgaaat ttgccgtcta tttgccaaat aatccagaaa
     6841 atcgaccgct tggtgtgatt tattggcttt ccggcttgac gtgtacggaa caaaatttca
     6901 ttaccaaatc aggctttcag cgttatgcgg cagaacatca agtaattgtg gtggcccccg
     6961 ataccagccc tcgcggagag caagtgccga acgatgatgc ttacgattta ggacagagtg
     7021 caggctttta tttgaatgcg accgaacagc cttgggcggc gaattatcaa atgtatgatt
     7081 acattttgaa cgagctgccc cgtctgattg agaaacactt tcctaccaac ggcaaacgtt
     7141 ccattatggg acattcaatg ggcggacacg gcgcattggt attggcgctg cggaatcagg
     7201 aacgttatca aagtgtttct gccttttcgc ctattttatc gccaagcctc gtgccgtggg
     7261 gagaaaaagc ctttactgct tatttaggga aagaccgtga aaaatggcag caatatgatg
     7321 ctaactcact cattcaacaa ggctataaag tgcaaggtat gcgcatcgat caaggcttgg
     7381 aagatgagtt tttgccgaca caattgcgta ccgaagattt tatcgaaacc tgccgtgcgg
     7441 caaaccagcc ggtcgatgtg cgtttccata aaggctacga tcacagctat tacttcatcg
     7501 ccagttttat tggcgagcat attgcttatc acgccgcgtt tttgaagtaa accaaagagc
     7561 gttcagtgtt caaagcagtt ttgggatagc cggcacgagg gcggtaagaa gtgccggcat
     7621 aaacgtatgc cgtctgagcc gaaaggagcc gactctacgg
//
LOCUS       DAWIVB010000097         7648 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWIVB010000097 DAWIVB010000000
VERSION     DAWIVB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.097"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(30..1232)
                     /gene="trpB"
                     /locus_tag="WIX60_001381"
     CDS             complement(30..1232)
                     /gene="trpB"
                     /locus_tag="WIX60_001381"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ5305642.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTLDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1315..2004)
                     /locus_tag="WIX60_001382"
     CDS             complement(1315..2004)
                     /locus_tag="WIX60_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ5305643.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDPVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            2201..2980
                     /gene="rsmA"
                     /locus_tag="WIX60_001383"
     CDS             2201..2980
                     /gene="rsmA"
                     /locus_tag="WIX60_001383"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ5305644.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKVV"
     gene            3153..3881
                     /locus_tag="WIX60_001384"
     CDS             3153..3881
                     /locus_tag="WIX60_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ5305645.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQGFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            3954..4313
                     /locus_tag="WIX60_001385"
     CDS             3954..4313
                     /locus_tag="WIX60_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305646.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     NMEEAELNITPVFDNMM"
     gene            4318..4764
                     /locus_tag="WIX60_001386"
     CDS             4318..4764
                     /locus_tag="WIX60_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ5305647.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGRMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRQCIMPDMTDKESYRKLKVWVLWRQPKKTAETDTSD"
     gene            4795..6069
                     /gene="folC"
                     /locus_tag="WIX60_001387"
     CDS             4795..6069
                     /gene="folC"
                     /locus_tag="WIX60_001387"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ5305648.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERTSEVKKRMKLEPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKIGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPKSLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLETVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6082..7080
                     /gene="ftsN"
                     /locus_tag="WIX60_001388"
     CDS             6082..7080
                     /gene="ftsN"
                     /locus_tag="WIX60_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ5305649.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINERLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7073..7570
                     /locus_tag="WIX60_001389"
     CDS             7073..7570
                     /locus_tag="WIX60_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ5305650.1"
                     /translation="MNSLPIADLLVSAVIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAASFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTSAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
BASE COUNT         1933 a         2165 c         1972 g         1578 t
ORIGIN      
        1 tgttaatcca ctatatcaga cggacgaggt tacagtttaa tccctttgag cttcgcgacg
       61 gtattgatgt ctttgtcgcc acgacctgag aggttgacca aaatcacttg gtctttaccc
      121 attttcggcg catttttcac cgcccaagca acggcgtggc tggattcgag cgcggggatg
      181 atgccctcga agcggcagag caagtcaaag gcttcgagtg cttcgtcgtc tttggcaaca
      241 gtgtattcga cgcgcttgat gtcgtgcaga tggctgtgtt ccgggccgat gccggggtaa
      301 tccaagcctg cggaaacaga gtgcgtaccc aaaacctgac cgttttcgtc ctgcatcaga
      361 tagctgcgga aaccgtgcaa tacgccaatc ggtgcgcccg aagtaatcgg cgcggcgtga
      421 tcgagggtgt tcacgcccaa accgccagcc tccacgccga cgaggcgcac gttttcttcg
      481 ccgatatacg ggtggaacaa accgatggcg ttcgatccgc cgcccacgca ggcaacggca
      541 acgtcgggct gtctgccgat ggcttcctgc atctgcgctt tagcttcgtt gccaatcacg
      601 cattggaaat cgcgcaccat ttcgggatac ggcgcggggc cggcggcggt accgatgatg
      661 tagaacgtgt cgtccacgcg ggcgacccat tcgcgcatgg cttcgttcat cgcgtctttc
      721 agcgtgcggc tgccgctttc tacaccgacc acgttcgcac ccaataattt catacggaac
      781 acgttgggca tttggcgttg gatgtcgtcc gcgcccatat acacgtcgca agtcataccg
      841 aagcgtgcgg caacggtggc actcgccacg ccgtgctgac ccgcgccggt ttcggcgatg
      901 acgcgttttt tacccatgcg ttttgccaac agtgcctgac cgatggtgtt gttgactttg
      961 tgcgcgccgg tgtggttcaa gtcttcgcgc ttcaaccaga tttgcgcgcc gcccagatgt
     1021 tcggacaacc gcgcggcgtg gtaaacgggg ctgggcctgc cgacataatg tttcaaatca
     1081 tggcggaacg cttcccaaaa ttcaggatcg tttttcgctg ccttataggc atccgccagc
     1141 tcttgcaagg cgggaatcag ggtttcggag acataaagcc cgccgtgttc gccgaaaaag
     1201 cccttctcgt caggcgcgtg gtagttcttc atcggattct tccttgtgct tcaaattttc
     1261 agacggcatt tccgtttttg caaacatgcc gtctgaacgg gaaattgtcg gcaattataa
     1321 ctgtttttca cacaagcccg ccatttcaaa aaacgcccga tacgccgccg cttttttcgc
     1381 caaactcaaa ggataggcgc gcgaagtcga aggcaggcgc gtcagcgtca aatcccgtcc
     1441 ggcaaacgga aacggcacgg tttcgcccgt tttcggcatt ttgataccgc cgccctgaat
     1501 atcgagcagg atttccgtcg ccttgccgcc tgtcgtacaa atatggcggc actcgggtat
     1561 ttttgccaac accgccgcca aatcgacggt ttcaactacc tttaaaaact tgtcggacgc
     1621 attgccgtgc tgacgtaccg ccttcaaaac ggtcggacag gacgcaatcc cccgttcgtg
     1681 caaaaacgcc ttgattttct cggcatcaaa cgctttttca gacaaccttt ggaaatgcgc
     1741 cgcatcatta aaaaacacca gcccataaac gcgccacata tcgttttgga aattcggata
     1801 atgaaactgc atcgcgcgtt tgtcttcctt gggcggaaac gtccccatca tcatgacagc
     1861 agccttcggc ggcaacacgg gatcgaaggg atgggtttcg atttcaagca gattttcaga
     1921 cacgttcaat cctccaacag gcaaaacggc agacagccgg tttttgcacc aaaccgccaa
     1981 acacggcaag ccttcaaaca gcattatcac gccctgccgt tttcggttac aatgccgaca
     2041 tcaaacggat actgtaaaca catttatgtt tcaacacaca ggacgacaca taaagcaccg
     2101 ccctatgtgc catcctgatt tggaaagagt tacgcccctc ccaaatagag tctgattcta
     2161 ccgccctaaa gggcggggtt tcaaccgaaa aggaaacacg atgaaagaac acaaagcccg
     2221 caagcgtttc gggcagaatt ttttgcagga cacgcggatt atcagcgata ttgtcaacgc
     2281 cgtgcgcccg caggcggatg atgtcgtgat tgaaatcggc ccgggtttgg cggcgattac
     2341 cgaacctttg gcgaaaaagc tgaaccgcct gcacgttgtc gaaatcgacc gcgacatcgt
     2401 atgccgtctg aaaacgctgc cgtttgcgga taaactggtg attcacgaag gcgatgtatt
     2461 gcagtttgat ttcaacggca tcgcaggcaa aaagaaaatc gtcggcaacc tcccgtacaa
     2521 catttccaca ccgcttttgt tcaagctggc ggaggtggcg gacgatgtcg tcgatatgca
     2581 ctttatgctg caaaaagaag tggtcgagcg tatggttgcc gcgccgaaaa gcaacgacta
     2641 cggccgcttg ggcgtgatgc tgcaatattt tttcgatatg gaaatgctga ttgacgtacc
     2701 gcccgaatcg ttcgaccctg cgccgaaagt ggattccgcc gtcgtccgta tgattccggt
     2761 gaaacaccgc atcggcaagg cggacgattt cgagcatttt gccaaactcg tgaaactcgc
     2821 cttccaccaa cgccgcaaaa ccatacgcaa caatctgaaa gagcttgcag gcgacgatga
     2881 tttgcaggca gtcggcatca atccgcaaga ccgcgccgaa catatcgcgc cagagaaata
     2941 cgtggcgttg agcaattatc tggcgggcaa ggtcgtctga aaagggagta agccgatgac
     3001 gaaatacctg cttttacccc tgatactgcc gcttgcagcc tgcctgacca atccgaacga
     3061 ccgttttttt gacggcagac gctacacgct tcatcatatg gagctcccgt aacgacaacc
     3121 tgcaacggca aatgccgtct gaaagaaaag acatgatcaa attcaaaaac gtacacaaac
     3181 atttcaaaga cctgcacgtc atcaacggcg taaacttgga aatcaaaaaa ggcgaagtgg
     3241 tcgtcgtctg cgggccttcg ggcagcggca aatccacact catccgcact gtcaaccagc
     3301 ttgaaagcat cgaaagcggc gagatttggg tggacggtgt caacgttgcc gaccccaaaa
     3361 ccgatttgaa caaaatccgc gaagaagtcg gcttcgtatt ccaaggcttc aacctctacc
     3421 cgcacctgac cgtgttggaa aacattaccc tcgcaccgat gaaggttaag gggcaaaatg
     3481 cggaacaggc ggagaaaaag gcaatggagc ttttggaacg cgtcggactg gcacacaaaa
     3541 aagacgcctt cccttcccaa ctttccggcg gccagcagca acgcgtggcg attgcacgcg
     3601 gtttggcgat ggaaccgcgc gtaatgttgt ttgacgaacc gacctccgca ctcgaccccg
     3661 aaatggtcgg cgaagtgttg aaagtgatga aagacttggc agaaagcggc atgacgatga
     3721 tgtgcgtaac ccacgaaatg gggtttgccc gcgaagttgc cgaccgcgtg attttcgtcg
     3781 ataaagggca aatcctcgaa gacgaaacgc ccgaagcgtt ttttaccaac ccgaaacacg
     3841 aacgcgccaa gcagttcctg caacaggtca tgacccactg atgctatacc gtctgaaaac
     3901 cgaacgttca gacagcatat cccgacaccg caagatgatt gagaaagaaa accatgtatt
     3961 ttgttgaccg caccgccgtc gtcctcaagc cgaccgcgcg ctttttggaa tggctcaaaa
     4021 gcaccgacga aaatatgccc gacctgacca tagagcagct tcgtgccaac tgttccgtct
     4081 tcctcgttcc gcagttcgac gaaccggaag ccgtcgtttc ctacttcgac gaacgctacc
     4141 ggcagatttt cgaggcggag ctggcaggtt gggacatcga taaggataaa tggccgcaag
     4201 atatggggct gaaggcgttt tgggaatttt tcgacatcga aatccacgat atggtgttga
     4261 acatggaaga agcggaattg aacattaccc ccgtgttcga caacatgatg taaggctgtg
     4321 cgcgcctttc agacggcatt gaggccgtcc cgcatcctga acatcctgac tgtatcgctt
     4381 cacgccgcct cccttgccgt ctgcctgact tggttttacg gccggatgat gtggttcggt
     4441 ttggcggctt tggtggcaag ttacgcatac tcgctcagga taacgaatct gaaacaccgc
     4501 catgccataa ccgccattac catagaccgc gacggacagg cggaaatcgt atccggcaaa
     4561 gacaaaacgg caaaggcggc ggcactgtcg ggcagcagta tggttacgcc ttatgcctta
     4621 ttcttacaat gggacaccgg cggcaaaacc gtccgccaat gcatcatgcc cgacatgacg
     4681 gacaaagaat cctaccgcaa gctgaaagtt tgggtacttt ggcggcagcc gaaaaaaacc
     4741 gccgaaaccg atacatcaga ctgatttcag acggcatttg aaaacgaaac actcatgaaa
     4801 acactacaag actggctctc gcatttggaa accgcccaca gcggcggttt gatcgacatg
     4861 ggtttggagc gcacgagcga agtgaaaaaa cgcatgaagc tcgagccgca atgccccgtc
     4921 gtcgttgtcg cgggaaccaa cggcaaaggt tcggtctgtg cctacctgac acagatttac
     4981 aaacaggcgg gctacaaaat cggtacgctg accagcccgc atttattgcg ttacaacgaa
     5041 cgcatcgcca tcaacgccga acccgtttcg gacgatacga ttattgcctc tttcgagcgc
     5101 atcgaagccg cgcgcggcga aatatcactg acttattttg aattcaatgc cttggcggct
     5161 gtcgacatct tcatgcgcga acaggttgac gtgatgatat tggaagtcgg cttgggcggc
     5221 cgcttggacg cggtcaacgc gtttgacggc gattgcgcgg tggttaccag cgtggatttg
     5281 gaccatcagg catttttggg cgatacggtc gagcaggtcg gctttgaaaa agcaggcgtg
     5341 ttccgtagcg gcaaacccgc catctgcggt caaaaccccg cgcccaaatc gttgctttca
     5401 cacgccgaag ccataggcgc gaaactgctg atggtgcagc gcgatttcga gtttcacgcg
     5461 atggaaaaca tccaatggaa ctaccgcttc cggccgcaac attcagacga ccccgcgcgc
     5521 aaccgcaatg ccctgccctt ccccgcattg cgcggcacat accagctttc caatgccgcc
     5581 tgcgcgctga ccgtattgga atgcttgaac gacaaattgc cggtggacat cggtgccatc
     5641 aagcgcggtt tgttgctggt tgaaaatccc ggacgcttcc aagtcctgcc cggccggccg
     5701 ctgaccgttt tggatgtcgg acacaatccc cacgccgccc gcgccctgcg ccgcagcctg
     5761 attaatttgg cgtttgcgca aaaacgcacc gccgtgttca gcatgttgtc cgacaaagac
     5821 atagacggcg tgttggaaac cgttaaagac cagtttgacg agtggtatat cgcaccgttg
     5881 gatgtaccgc gcggcatgac gctcgagagc ctgcaaaaca aactgcacga acacagcatc
     5941 gaaaacatac aaacattcgc cgccgtccgc gaagcatacc gggcggcagc ctcaaaagcg
     6001 ggcgagaacg acagaatcgt cgttttcggc tcatttcata ccgttgccga tgtcatgtcg
     6061 gtactttaaa aggaaacttc tatgtccgaa aacaaacaaa acgaagtcct gagcggttac
     6121 gaacaactca aacggcgcaa ccgccgccgc ctcgtaacgg caagttgcct ggttgccgcc
     6181 tcctgcatcc tgctggcagc cgccctcagt tccggccctg ccgaacagac tgccggcgaa
     6241 acaagcggcg tagaaaacaa agcggcaggt gcggcacaaa cccctgcctt gaaatccgcc
     6301 gccgacaaac cgcaggactt ggcaggcgaa gacaagcctt ctgccgccga cagcgaaatc
     6361 agcgagcctg aaaacgtagg cgcgccgctg gtgctgatta acgagcgcct cgaagacagc
     6421 aacatcaaag gtttggaagc atccgagaaa ctgcaacagg cagaaaccgc caaaaccgca
     6481 ccgaagcagg caaaacaacg cgctgccgaa aaagtgccgg caactgccga cagtacggat
     6541 acggtagcgg ttgaaaaacc gaaacgcact gccgaaacaa aaccgcaaaa agcggaacgc
     6601 actgccaaag ccaagcccaa agccaaagaa accaaaaccg ccgaaaaagt tgccgacaaa
     6661 ccgaaaactg ccgccgaaaa aaccaaaccg gatacggcaa aatccgacag cgcggtaaaa
     6721 gaagcgaaaa aagccgacaa ggctgaaagc aaaaaaacag ccgaaaaaga ccgttcggac
     6781 ggcaaaaaac acgaaacggc acaaaaaacc gacaaagcgg acaagaccaa aaccgccgag
     6841 aaggaaaaat ccggtaaaaa agccgccatt caggcaggtt atgccgaaaa agaacgcgcc
     6901 ttaagcctcc agcgcaaaat gaaggcggcg ggtatcgatt cgaccatcac cgaaattatg
     6961 accgacaacg gcaaagttta ccgcgtcaaa tcaagcaact ataaaaacgc aagggatgcc
     7021 gaacgcgatt tgaacaaatt gcgcgtacac ggtatcgccg gtcaggtaac gaatgaatag
     7081 cctccccatt gccgacctcc tcgtctccgc cgtcatcgcc gcctgcatcg tgctatccgc
     7141 gatgcgcggc gtgattgcgg aggcaggctc aatggcggca tgggtggttt ccttcttttt
     7201 cgccaaactc tttgccgcct ccttcgccga cctcgccttt gcctcgttcc aaccccgcct
     7261 gtttgcattg gctctgtcgt tcatttccct gttcgtcatt gcctgtctga tccagaaaat
     7321 gctccgttcg ctgctgacca gcgcagtttc ggcggtcggt ttgggctttg ccaaccgcat
     7381 tttgggcggc gtattcggtg cattgaaagg cgttttgatt gttaccctgc tggtcatgct
     7441 tgcttcaaaa accgacctgc ccgataccga agaatggcgg caatcttaca cactgccgtt
     7501 tttcgtatcg ctttccgaag ccgtgttgaa ccatagcggc ggcacggcgg aaactccgga
     7561 agacgattga ttgccatacg gatatttcag acggcatcct cttttatgcc gtctgaaatg
     7621 ttttttcctt tcctcagcca tatcacag
//
LOCUS       DAWIVB010000098         7619 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWIVB010000098 DAWIVB010000000
VERSION     DAWIVB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.098"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(100..1017)
                     /locus_tag="WIX60_001390"
     CDS             complement(100..1017)
                     /locus_tag="WIX60_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5305651.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            1190..1990
                     /locus_tag="WIX60_001391"
     CDS             1190..1990
                     /locus_tag="WIX60_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5305652.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            2038..2814
                     /gene="mlaE"
                     /locus_tag="WIX60_001392"
     CDS             2038..2814
                     /gene="mlaE"
                     /locus_tag="WIX60_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ5305653.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            2865..3359
                     /gene="mlaD"
                     /locus_tag="WIX60_001393"
     CDS             2865..3359
                     /gene="mlaD"
                     /locus_tag="WIX60_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ5305654.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            3396..3986
                     /locus_tag="WIX60_001394"
     CDS             3396..3986
                     /locus_tag="WIX60_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ5305655.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            4037..4315
                     /locus_tag="WIX60_001395"
     CDS             4037..4315
                     /locus_tag="WIX60_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ5305656.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            4312..5139
                     /locus_tag="WIX60_001396"
     CDS             4312..5139
                     /locus_tag="WIX60_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ5305657.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            5264..5626
                     /locus_tag="WIX60_001397"
     CDS             5264..5626
                     /locus_tag="WIX60_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ5305658.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            5623..6009
                     /locus_tag="WIX60_001398"
     CDS             5623..6009
                     /locus_tag="WIX60_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ5305659.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            6006..6494
                     /locus_tag="WIX60_001399"
     CDS             6006..6494
                     /locus_tag="WIX60_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ5305660.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(6575..6742)
                     /locus_tag="WIX60_001400"
     CDS             complement(6575..6742)
                     /locus_tag="WIX60_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ5305661.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(6709..7089)
                     /locus_tag="WIX60_001401"
     CDS             complement(6709..7089)
                     /locus_tag="WIX60_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ5305662.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            7099..7225
                     /locus_tag="WIX60_001402"
                     /pseudo
     CDS             7099..7225
                     /locus_tag="WIX60_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7327..7542
                     /gene="rpmE"
                     /locus_tag="WIX60_001403"
     CDS             7327..7542
                     /gene="rpmE"
                     /locus_tag="WIX60_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ5305663.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         1785 a         2205 c         1930 g         1699 t
ORIGIN      
        1 ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc
       61 actatacacg cggatttcgg gtttgaaatc aaatgcggct taccggtttt cttttttggt
      121 ttccgcaggc gtgctgccga aatattgcct gaatgcctga gtaaagctgg aaacgtggcg
      181 gtagccgcat aaatatgcgg tttcgcctat gctttttccc ccgttttgca acagatagag
      241 cgcgtgctgc atttgtttgt ggtgcagcca ttcgcttgcc gtaatgccga aatggtcgcg
      301 catacggcgt tgcagcgtcc tttcactgat gttcagcgcg tctgtcagcc ggttgacttg
      361 gtgtgcgccg tcggcaaacg cggcatttag ggtgcggctg aagtcttcag acggcatagc
      421 gtctgcttcc gccgtttgcc ccgccgccgg cccgatgccg tctgaaaccg tgtcccacaa
      481 gtccgacagc agccgcaaca cgtccgcctc gcggcgcaat gtttcgccca aatgcccctt
      541 tgggacggct ttcaggcagg atgccgccaa gccgcgcagg ttcgggggca aatcccatat
      601 cctgaccggt tcgcggtaaa gcaggggtgc gaaacgcgcg tattccggac gcagcagcca
      661 ttgttccata cctttaatgg tcattttgac cgttttgccg cctcggtaaa gatagcggct
      721 gaacaggact tcttccccga cagcaattag gacaatcttg ccgccgtccg catcgatttg
      781 gaagcggcag cggttgatgc cgaagtccaa actgccttcc agcaagagca cgaacgacac
      841 ataaggttcg gcgaggcggc tgctgcaaaa atcacaccgt gccgttactg tgccgccgtg
      901 gatggaaatg ccgttggaca aagtatcgaa acggtagctg ccctccacat agctgccgcc
      961 gccgcttttg actagattga cgaaccggct gctgttcaaa tgttctgctt tttccatagt
     1021 tttctgataa aggttatcat ttgaaagata acatttttcg ccacagcaaa caatccgccg
     1081 ccgacaacgg cattttcaca acatttaccg tccgtatgcc gtctgaaaat tgcccgtctt
     1141 cccgataagc cttaaaatac acgccgtcaa acctaccgtc cgtccgtaca tgagcccatc
     1201 cccctttatc gaaatgaaag acgtcgcctt cgcgtatggc gaccgcccga ttctgaagaa
     1261 catcaatttc agcattccgc aaggcaattt tgctgccgta atgggcggtt cgggcagcgg
     1321 caaaaccacg ctgatgcgac tgattacagg acagattcgt ccgcagtccg ggcaggtttt
     1381 gattgaagga cgggatttgg cgggcttttc ggctgacgag ttgtatgaac accgccgccg
     1441 tatgggcgta ttgttccaac acggcgcgct gtttactgat ttgtcggtat tcgacaatat
     1501 cgcttttccg atgcgcgaac tgacgcgcct gccggaagcg gtgattcggg atttggtttt
     1561 gttgaaattg aacgcggtcg gtctgcgcgg tgtggaaaac ctgatgccgt ccgagttgtc
     1621 cggcgggatg tcgcgccgtg tcgcgcttgc ccgcacgatt gcgctcgacc ctgaaattat
     1681 gttgtacgac gagccgttta ccggcctcga tccgatttcc ttgggcgtga ttgcccactt
     1741 gatcagccgc gtcaacaagg ctttgcgttc gaccagtatt atggtaacgc acgacattga
     1801 aaaatctttg gaaatcgtcg atcaggtgat tttcttggcg cacggcgaaa ttatgttctc
     1861 cggctcgccg caggaaatgc gcgaactgga ttcgccttgg gtgcgccagt ttgtcggcgg
     1921 gctggcagac ggccccgtag cataccgtta tccggcgcaa acgtcgttgc agcaggacct
     1981 gttgggataa atgagttgat gccgtctgaa accgttgaac tttacacata gaaatttatg
     2041 aactttatcc gttccgtcgg ggcgaaaacc ctcggcctta ttcaatccct cggcagtatc
     2101 acgctgtttc tgctgaacat tctggcgaag tccggtacgg ctttcgtccg tccgcgcctg
     2161 agcgtgcgcc aagtgtattt cgccggtgtg ctgtcggtgt tgattgttgc cgtttcgggg
     2221 ctgtttgtcg gcatggtctt gggtttacag ggctatacgc agttgtcgaa attcaaatcc
     2281 gccgatattt tgggctatat ggtcgcggct tcgctattgc gcgagctggg tccggtattg
     2341 gcagcgattt tgtttgccag cagcgcgggc ggtgcgatga ccagcgaaat cggtctgatg
     2401 aaaacgaccg aacagctcga agcgatgaat gtgatggcgg taaaccctgt cgcccgcgtg
     2461 gttgcgccgc gcttttgggc gggcgtgttt tccatgccgc ttttggcttc gattttcaac
     2521 gtggcgggca ttttcggtgc gtatttggtt ggtgtaacct ggctgggctt ggacagcggt
     2581 attttctggt cgcaaatgca gaacaacatc acgatacatt acgatgtaat caacggtctg
     2641 atcaaatccg ccgcgttcgg cgtggcggta acgctgattg ccgtgcatca gggcttccat
     2701 tgcgtcccga cttcggaagg cattttgcgc gccagcacgc gcacggtggt ttcgtccgcc
     2761 ctgacgattt tggcggtcga ttttatattg accgcgtgga tgtttacaga ttgacaggcc
     2821 gtctgaagac gaaacgcgga acatcggata ttcaaggaac tttaatgaaa aagaacatat
     2881 tggaattttg ggtcggactg ttcgtcctga ttggcgcggc ggcggttgcc tttctcgctt
     2941 tccgcgtggc cggcggtgcg gcgttcggcg gttcggacaa aacttacgcc gtttatgccg
     3001 atttcggcga catcggcggt ttgaaggtca atgcccccgt caaatccgca ggcgtattgg
     3061 tcgggcgcgt cggcgctatc ggacttgacc cgaaatccta tcaggcgagg gtgcgcctcg
     3121 atttggacgg caagtatcag ttcagcagcg acgtttccgc gcaaatcctg acttcgggac
     3181 ttttgggcga gcagtacatc gggctgcagc agggcggcga cacggaaaac cttgctgccg
     3241 gcgacaccat ctccgtaacc agttctgcaa tggttctgga aaaccttatc ggcaaattca
     3301 tgacgagttt tgccgagaaa aatgccgacg gcggcaatgc ggaaaaagcc gccgaataaa
     3361 cgtatgccat catattcaaa ctaggacact taatcatgaa aaaatcctcc ctcatcagcg
     3421 cattgggcat cggtattttg agcatcggca tggcatttgc cgcccctgcc gacgcggtaa
     3481 gccaaatccg tcaaaacgcc actcaagtat tgagcatctt aaaaaacggc gatgccaaca
     3541 ccgctcgcca aaaagccgaa gcctatgcga ttccctattt cgatttccaa cgtatgaccg
     3601 cattggcggt cggcaaccct tggcgcaccg cgtccgacgc gcaaaaacaa gcgttggcca
     3661 aagaatttca aaccctgctg atccgcacct attccggcac gatgctgaaa ttaaaaaacg
     3721 ccaacgtcaa cgtcaaagac aatcccatcg tcaataaagg cggcaaagaa atcatcgtcc
     3781 gcgccgaagt cggcgtaccc gggcaaaaac ccgtcaacat ggacttcacc acctaccaaa
     3841 gcggcggtaa ataccgtacc tacaacgtcg ccatcgaagg cgcgagcctg gttaccgtgt
     3901 accgcaacca attcggcgaa attatcaaag cgaaaggcgt ggacggactg attgccgagt
     3961 tgaaagccaa aaacggcggc aaataaccga caaaccatac cgtccgaggg cggtatgggt
     4021 tttccgagca aacgctatgc atacagaact caaaaacgga atattgcaca tcggcggcga
     4081 catcaccgtc aaaaccctga ccgccgccgc ttttacccgc ttcagacagc aatgtcgtct
     4141 gaaagacacc tgcgcggttg atttgagcgg cgtcggacgc gccgattccg cctgcgtgtc
     4201 gctgctgctc gaagtgctgc gcggttgcaa aggcagcgtc aggctgaccg gcattcccga
     4261 atccgtgcgc gcgctgtccg aactgtacga aatcaaagac tggctgaaat catgaaaaaa
     4321 accgcctatg ccttcctcct gctgatcggg ttcgcttccg cccctgcatt tgccgaaacc
     4381 cgccccgccg acccttatga aggctacaac cgcgccgttt tcaaattcaa cgaccaagcc
     4441 gaccgctaca ttttcgcccc tgccgcgcgc ggctaccgca aagttgcgcc gaaacccgtc
     4501 cgcgccggcg tgtccaattt ttttaacaac ctgtgcgacg tggtcagctt cggcagcaat
     4561 atcttgcgct tggacatcaa acgcgcaagc gaagaccttg tccgcgtcgg catcaacacc
     4621 actttcggtt tgggcgggct tatcgacatc gccggcgcgg gcggcattcc cgacaataaa
     4681 aacaccttgg gcgacacgtt tgcctcgtgg ggatggaaaa acagcaatta tttcgtgttg
     4741 cccgtcttag ggccgtccac cgtccgcgac gcgctcggca cgggtattac ctccgtttat
     4801 tcgcccaaga atatcgtctt ccgcacccct gtcggacgct ggggcacgac tgccgtatcc
     4861 gccgtcagta cgcgcgaagg cctgctcgat ttgaccgaca gtctggacga agccgccatc
     4921 gacaaataca gctacacgcg cgacctctat atgaaagtcc gtgcgcggca gaccggtgca
     4981 acacctgccg aaggtacgga agataacatc gacatcgacg aattggtcga aagtgccgaa
     5041 accggcgcgg cggaaactgc cgttcaagaa gattccgtat ccgaaacaca ggcagaagca
     5101 gcaggggaag ccgaaacgca acctggaaca caaccttaaa gccatgccgt ctgaaaatcc
     5161 gtgttcggac ggcattccct tctgtcggaa cggacagtcc gaaaccacag attgggctat
     5221 aatctctttt ttgcgcatac cgcgcgacag caaaggaaaa accatgtacg aagtcaaccg
     5281 cagcgtattc gtcctcatcc ctttagaacc tttttggaac tggctgcaaa ccctgccggg
     5341 caaccacctc gacggactga ctcttgaaga catccaagcc gacgccaatt cctaccttgt
     5401 ccgcccgtgc gaaaccgccg acgaagtatg ggacgaaatc gaagcccgtt ttgaagacat
     5461 tttcgccgcc gaacttgccg actggtgcga agacgaacgc gagtggcctg cgctcgatgc
     5521 cgacattttc aacgaatggt tcgacatcca gctttctacc gtcatcaccg acctcgaaca
     5581 cgaaccgctt gcccgcgaag ccttccaacc catcaacctg aactgatgaa gctgaccgtc
     5641 cgcaactacc atctcgacgg ctacggccat gtcaacaatg cgcgctacct cgaatttttc
     5701 gaagaagcgc gctgggcgtt ttttgaagaa cgcggactgc tgcacgagtt ggcaggcctc
     5761 atactgattg tcgcccgcat cgacatccga tacagccgcc ccgccgtcga aggcgacgta
     5821 ttgcagtttt catgccgcct gaaaaccccc ggcacgcgcc gtattgtgct gacccagacg
     5881 attacactgc caaacggcaa aaccgccgca gaagccgaca tcaccctgat gcccgtccac
     5941 gccgccacac aacgcaccgt cagcctgccc gccacccttg cccgcgcatt ggaagccttg
     6001 tccgaatgaa aaaaatactc accgccgccg tcgtcgcact gatcggcatc ctccttgcca
     6061 tcgtcctcat ccccgacagc aaaaccgcgc ccgccttctc cctgcccgac ctgcacggaa
     6121 aaaccgtttc caacgccgac ctgcaaggca aagtaaccct gattaatttt tggtttccct
     6181 cctgtccggg ttgtgtgagc gaaatgccca aaatcattaa aacggcaaat gactataaaa
     6241 acaaaaactt ccaagtactt gccgtcgccc agcccatcga tccgatagaa agcgtccgcc
     6301 aatatgtcaa agactacggt ttgccgttta ccgtcatgta tgatgcggac aaagctgtcg
     6361 gacaggcgtt cggcacacag gtttatccga cttccgtcct tatcggcaaa aaaggcgaaa
     6421 tcctcaaaac ctacgtcggc gaacccgatt tcggcaaact ctaccaagaa atcgataccg
     6481 cgctggcgca atagcgatgc cgtctgaagc gcaaaaaaca gaaacgatgt tcctgttttt
     6541 tcagatcatc cgccccgaca cgttcagacg gcattcaacc ttccggcaga ttctttttca
     6601 ccactgccgg attgcctgcc gccaaagagt agggcggaat gtcttttgta accaccgcgc
     6661 ctgcgcccac gaccgaaccg cgtccgacgg ttacgcccgc cattacaatc accctgtgcc
     6721 ccggccagac atcgtcctcc aacgtaatcg gacggatttc cgtgtagccc tcaaagcgtt
     6781 ttttgaacgg tcaaacttgt ggttatttga ataaaacaga cattccggct ccatcatcac
     6841 atttttgccg accaccagcc cacggcagat ttcacagttt gccccgatgc ccgagccgtc
     6901 gcccaaaacc gtatccggaa acacatacgc cccgcgttcg atattgaccc cgcgtccgat
     6961 atgcggagaa acccgccgcg ccaaaaaacc gcgcacccgt ctgccgacga tgccgatgcc
     7021 gcgcatatgt gacggcggca accccgaacc ggcaaaacgc agcagcagga aaaagaattt
     7081 tgatcccatc aaacctccct caaaagaact ttttatagtg gattaacaaa aattaggaca
     7141 aggcaacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg ctgtactggt
     7201 ttttgttaat ccactataca ttttatcgaa tatactcttg acgtaacaac agaaaatagc
     7261 gagaatgtcg gacttgtctg tagcataggt acatccccta tgcccgcttt tttgaaagga
     7321 aatcaaatga aacaaggtat tcacccgaac taccacgaag tcaacgttac ctgctcttgc
     7381 ggcaacaaat tcgcaaccaa atccgcgatg gaaaaagaaa acttcaatat cgaggtttgc
     7441 tccctgtgcc acccgttcta taccggcacc caaaaaatcg ttgataccac cggccgcgtg
     7501 gacaaattca acaacaaatt cggcaacctg ttcaaacgct gattgccgct ttgcaagaat
     7561 taagccctgc catcatttcg atggcagggc tttttaatat ttggcacact gacttctta
//
LOCUS       DAWIVB010000099         7592 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWIVB010000099 DAWIVB010000000
VERSION     DAWIVB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.099"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(53..361)
                     /locus_tag="WIX60_001404"
     CDS             complement(53..361)
                     /locus_tag="WIX60_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305664.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDALKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WIX60_001405"
     CDS             complement(345..647)
                     /locus_tag="WIX60_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ5305665.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WIX60_001406"
     CDS             complement(721..3084)
                     /locus_tag="WIX60_001406"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ5305666.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGEVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     CVFEIARVFSKGSDGQFVQNERIGGLWYGAVMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRVVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3109..3759)
                     /locus_tag="WIX60_001407"
     CDS             complement(3109..3759)
                     /locus_tag="WIX60_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ5305667.1"
                     /translation="MITISNEDNMILMSRYPDKYFDLAIVDPPYGILNKTKRGGDYKF
                     NMNEYSQWDIKPDQTYFNELFRVSKNQIIWGGNYFGELWLRSEYNKGFIIWDKNQPET
                     LNNFSMAEMAWSSFDRPSKIFRFSVRKNRNKTHPTQKPVELYQWLLKMYAKQGDKILD
                     THLGSGTLAIACCIAQFDLTACEINSDYYQQSIEKIKNNLPEARISFGHPGYCIIE"
     gene            complement(3756..5225)
                     /locus_tag="WIX60_001408"
     CDS             complement(3756..5225)
                     /locus_tag="WIX60_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ5305668.1"
                     /translation="MTTARWVIDKHLQNFHILCKFAGILKTNSFISVEDKAKLSEKLE
                     KLDLYHRRNTGKSLDATTHKIKELSFYMFGYRDVCGQVTQKFLFSPLGNLFLKHLDNN
                     EYIQKIFLTMLWAIPFPHPYIKTDESIQLYPMRLIFKLLSDERLDCKLFSYEYIYLIS
                     FVKSADQNSYEKLVQDILVLRTCAEVKIKHQLTAENSRSHAYVNAAHEWESYFSKTLT
                     DAGVLQKTDGKIICRLKHGKTETYRKVTSSEFSIPKQLQEFVKKLQSAYSFSEMPLNL
                     NDSDRLKIDVIKEIYSFYPKELLEEIGELKDEAAYELLHLPRLIEQYADNNNGTEAYL
                     FEDVLEMGFNMFYNVEAKKIGGPGNTDLECLYITQKRKFAVEAKSTKNKLSGINSGRL
                     EDHKNKIKAIYTIVVTPRYVPAVLSDIRNCPIVIIRANTFAEFLYNCLINRSSIPEID
                     YRYFDEIIIKNLGKDISSEISNLTMQQFASNTTMEAYST"
     gene            complement(5228..6286)
                     /locus_tag="WIX60_001409"
     CDS             complement(5228..6286)
                     /locus_tag="WIX60_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ5305669.1"
                     /translation="MMIGASLFSSAGIAETYLHNAGIKIIAANELVPERANLYKALYP
                     ESKMIIGDILHEEVFQNLIQSVPNRLDFLIASPPCQGMSVAGKNRNIQEMANDKRNHL
                     IFRVIEFILLKRPTFVLIENVPFFLKIKLPYKGTLQTVEVILQDLFGCEYYIQTHIFD
                     SADYGVAQHRKRAIIRMNKHSTIWGMPEKVTKTISVRDAISFLPSIESGQKSNVKWHF
                     ARTHAPEHIIWLKNTPTGRSAFDNIEHYPKKKNGEKIKSYNTTYRRMEWDAPAPTITI
                     RNDAISSQLNVHPGRSMPDGTYSDARVLTPLELMLLTSLPIDWNIPDDTSELLIRQCI
                     GESIPPLLIKKIVERIGK"
     gene            complement(6469..7461)
                     /gene="pheS"
                     /locus_tag="WIX60_001410"
     CDS             complement(6469..7461)
                     /gene="pheS"
                     /locus_tag="WIX60_001410"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ5305670.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKREALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFAK"
BASE COUNT         2069 a         1808 c         1498 g         2217 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagagcgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg atcatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgactgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacga cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cacgtccggg atgcagggcg ggatgtccgg ttttaacgaa
     1201 ctcgactgct ttgtttttca acagattttc cacgtccgcc ttgatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttccgg catgaccgcg ccgtaccaca atccgccgat
     1321 gcgttcgttt tggacaaact ggccgtctga acctttgctg aacacgcggg cgatttcaaa
     1381 cacgcacacg cggttttgtt tgcggttcag attgttttgc agaatttcca ccaagccgcc
     1441 gatgagcgtg gaacgcatca cggcatactg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtaa ccgcgagccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggtccaa
     1861 acgcaatcca acctgcttcg cttcaggcaa ttcgccttgc gcttccacca tctcgcctgc
     1921 cgcaccaccg caaatctgca acaccaattc ggtagcacgt tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatccg gcgtagtagc
     2401 gcgcgcgttc acgttttcaa tcacacggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacacctcgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcagcgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttca gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatggttaa tttctctata tttttaagtt attcaataat
     3121 acaataacct ggatgcccaa aactgattct agcttcaggt aaattatttt ttattttctc
     3181 aatcgattgt tggtaataat cggaattgat ttcacaagct gtcaaatcaa actgtgcaat
     3241 gcagcatgca atagcaagag ttccacttcc taaatgtgta tctaaaatct tatcaccctg
     3301 ctttgcatac atttttaaca accactgata taattcgact ggtttttgtg ttgggtgagt
     3361 tttattacga tttttccgca cactaaaccg gaaaatttta gatggcctat cgaatgacga
     3421 ccaagccatt tccgccatag aaaaattatt taatgtctct ggttgattct tatcccaaat
     3481 aataaatcct ttattatatt cacttctcaa ccataactcg ccaaaataat tcccacccca
     3541 aataatttga ttttttgaca cgcgaaataa ttcattaaag taagtttggt ctggcttaat
     3601 atcccattgt gagtattcat tcatattgaa tttataatca ccaccacgtt tagttttatt
     3661 caaaatccca taaggaggat ctacaattgc caaatcaaaa tacttgtcag gataccgaga
     3721 cattaagatc atgttatctt catttgaaat agttatcatg tactatacgc ttccattgtg
     3781 gtgttacttg caaactgttg catagtcaaa ttggaaattt ctgaactaat atcttttcca
     3841 agatttttaa taataatttc atcaaaatac cgataatcaa tctctggaat actggagcga
     3901 ttaatcaaac aattatataa aaattcagca aatgtattgg cacgaataat tacaattggg
     3961 caattacgaa tatcggataa tacggcaggg acataacgtg gtgtgacaac aattgtgtaa
     4021 atggccttaa ttttattttt atgatcttcc aatcttcctg aattaatacc tgataactta
     4081 tttttagttg attttgcctc cactgcaaat tttctctttt gcgtaatata taagcactct
     4141 aaatccgtat tacctggtcc accaattttt ttagcttcta cgttataaaa catattgaac
     4201 cccatttcta gaacatcttc aaatagatat gcctctgttc cattattatt atctgcatat
     4261 tgttcaatca acctaggtaa gtgcaataat tcatatgctg cttcatcctt aagctcacca
     4321 atttcctcca ataactcttt tggatagaag ctataaattt ccttaatgac atcaattttc
     4381 aaacgatcac tatcgttcag atttaatggc atttctgaaa acgaataagc actttgcaat
     4441 tttttcacaa attcctgaag ttgcttagga atcgaaaact cacttgatgt tactttacga
     4501 tatgtttcgg tcttaccatg ctttagacgg caaataattt taccatctgt tttttgcaaa
     4561 acacctgcat cagtcaatgt ttttgaaaaa taagattccc actcatgtgc tgcatttaca
     4621 taagcatgac tacgactatt ttccgcagtt aattgatgtt taatttttac ttcagcacat
     4681 gttcgtaaca ccaaaatgtc ttgtactaat ttttcatagc tattctgatc agcagatttc
     4741 acaaatgaaa ttaaatagat atattcataa gaaaatagtt tacaatccaa tctttcatca
     4801 gataacaact taaatattag tctcatggga tataattgaa tactttcatc agtcttaatg
     4861 tacggatgag gaaatggtat cgcccacaac atagtaagaa aaattttttg aatatattca
     4921 ttattatcca agtgtttcaa aaataaatta cccaatggac tgaacaggaa tttctgtgta
     4981 acttgcccac acacatcacg ataaccaaac atatagaatg ataattcttt tattttatga
     5041 gtagtagcat ccaatgattt acctgtattt cgtctatggt ataaatctag tttttccaat
     5101 ttttcagata acttagcttt atcctctaca gatataaaag aatttgtttt caaaatacct
     5161 gcaaatttac ataaaatatg aaaattctgt aaatgtttat ctattaccca gcgcgcagtt
     5221 gtcatatcta ttttcctatt ctctctacaa tttttttaat taacaatgga ggaatagatt
     5281 caccaataca ttgccgaatt aataattctg atgtatcgtc gggaatattc caatcaatag
     5341 gtaatgatgt taaaagcatt aactctaatg gtgttaaaac tcttgcatct gaatatgttc
     5401 catcaggcat agaccgtcca ggatgaacat ttaattgtga actgatagcg tcattacgaa
     5461 tagtaatagt tggggcagga gcatcccact ccatacggcg ataagttgta ttataacttt
     5521 taattttttc accatttttt ttctttggat aatgttctat attatcaaaa gcagatcgtc
     5581 ctgttggcgt attttttagc catataatgt gctccggagc atgtgtacgt gcaaaatgcc
     5641 atttcacatt agacttttgt ccagactcaa tactaggcaa aaaactaata gcatcacgaa
     5701 cagaaatggt ttttgtaact ttttccggca ttccccaaat agttgaatgt ttattcatac
     5761 gaataatagc tcgtttacga tgttgtgcaa caccataatc ggcagaatca aaaatatgag
     5821 tttgaatata atattcgcaa ccaaataaat cttgcaaaat tacttctact gtttgtaatg
     5881 tccccttata aggtaactta atttttaaaa aaaatggaac attttcaatc aaaacaaaag
     5941 ttgggcgttt taataaaata aattcgatta ctctgaatat taaatgatta cgtttatcat
     6001 tagccatctc ttgaatgtta cgatttttcc ctgcaacact catgccttga caaggaggag
     6061 aagcaattaa aaaatctaat cgattcggca cgctctgtat taaattttga aacacttcct
     6121 catgaagtat atcacctata atcattttac tttcgggata tagagcttta tataaattag
     6181 cacgttctgg caccaattca tttgcagcta taatcttaat acccgcatta tgcaagtagg
     6241 tttctgcaat ccctgcactc gaaaacaaag aagcccctat catcatagaa ccaaacccaa
     6301 attaactata taatctctac aaagcaataa attatgcctc tttaaacaaa attaggaatg
     6361 taatttcaca acaaaatatc aaatgtttta tatcttacat ttgaattttc ctaagcagcc
     6421 tgaaaaccaa gagtaggctg cttttccata ttcaggcagt ctgcacgatc atttcgcaaa
     6481 ctgcttcaaa aagttcaaat cattatcgaa gaacaggcgc aagtcgttca cgttgtaacg
     6541 cagcatagcg aagcggtcga gaccaatacc aaaggcgaaa ccggtatatt tttcagggtc
     6601 gatattgacg tttttcaaca cgttaggatg taccataccg caaccgccta cttccagcca
     6661 tttgccgttt tcgcccataa tgtcgatttc ggcagacggt tcggtgaacg ggaagaaaga
     6721 cgggcggaaa cgtacttgca aatcatcgcg ttcaaagaag cgacggataa aatccgtgaa
     6781 cactgctttt aagtcggcaa aagttacgcc ctcttctacc cacaaacctt ccgcctgatg
     6841 gaacataggc gagtgcgtgg catcgctgtc cacacggtaa acgcggccgg gggcgataat
     6901 gcggatgggc ggctcttttt tatcgagcat atagcggatt tgaatcgggg aagtgtgcgt
     6961 acgcaaaaca tcgccgtttt caacgtaaaa cgtatcctgc atcgcacggg caggatggtt
     7021 tgcagggatg ttcagggctt ggaaattgtg aaaatcgtct tcgatttcag gcccgtccgc
     7081 cacttcgaaa cccattccgt gaaagagttc gaccacacgt tgcaaggtca gggttacggg
     7141 atgcaggctg ccgccttcct gagcgcgtcc gggcagggta atatcgaggg cttcggcggc
     7201 aagtcgggct tgcagcttga cttcgttgag ggcttcgcgt ttggcattaa aagccgtctg
     7261 aaaccggttt ttgcattcat tgatatgcgc acctatggtt ttgcgctctt caggcgacat
     7321 ttgccccaaa gttttcagaa gtccggtcaa ctcgccggtt ttaccaagat aacgggcttt
     7381 gatttgttct agagcgttga agtcttgcgc agcttctact gcggcaatgc cttctgcaac
     7441 gatgcggttt acattttcca taatatcaag tctgtcaatt aatgatatac cacctgaaac
     7501 aacgaggccg cctgtatagc ctgacatatc agactcttca gacgacctta ttttcttcac
     7561 ggtcaatcca ttggataaat tttctgtata gt
//
LOCUS       DAWIVB010000100         7527 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWIVB010000100 DAWIVB010000000
VERSION     DAWIVB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.100"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            15..420
                     /locus_tag="WIX60_001411"
                     /pseudo
     CDS             15..420
                     /locus_tag="WIX60_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WIX60_001412"
     CDS             complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WIX60_001412"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ5305671.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            1376..1723
                     /locus_tag="WIX60_001413"
     CDS             1376..1723
                     /locus_tag="WIX60_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ5305672.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="WIX60_001414"
     CDS             1728..2321
                     /locus_tag="WIX60_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ5305673.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="WIX60_001415"
     CDS             2385..2993
                     /locus_tag="WIX60_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ5305674.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3633
                     /locus_tag="WIX60_001416"
     CDS             3001..3633
                     /locus_tag="WIX60_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305675.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     AAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3673..4452
                     /gene="map"
                     /locus_tag="WIX60_001417"
     CDS             3673..4452
                     /gene="map"
                     /locus_tag="WIX60_001417"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ5305676.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4607..4906
                     /locus_tag="WIX60_001418"
     CDS             4607..4906
                     /locus_tag="WIX60_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ5305677.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5144..5518
                     /locus_tag="WIX60_001419"
     CDS             5144..5518
                     /locus_tag="WIX60_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ5305678.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTDNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5706..7172)
                     /locus_tag="WIX60_001420"
     CDS             complement(5706..7172)
                     /locus_tag="WIX60_001420"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ5305679.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1927 a         2264 c         1851 g         1485 t
ORIGIN      
        1 aagaggcgga atgggtgggc agcctgttcg gcctgcacaa atctttcggt ttccttacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa
      121 gcgactgcaa ggctgcggcg gcaggccacg gcattctgta tctgctcatg cttgctgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaagtgat gcaagggttc gccggaaaaa atcgagtgga tggcaaactt gggcaacacg
      301 ttccacggca atttgggctt gctgctgttt gccgccgtcg ccggacacgt cgccatggtc
      361 gtcgcccacc gtgttcaggg tagagatgtt ctgtgccgca tgacgggtcg tgtccgctga
      421 ttccgttcac actatggtgc cggctcgtcc ggcactattt gtttttccaa gacagagcca
      481 gatcgtacaa agctttcttt ccctcgcccg tgattttggc agcaagctcc gccgcctgtt
      541 tggtcggcag ctcggctgtg aggattttca tgatgttttg cgcggactcg gacaagcctt
      601 cgtgtttttc atcctgcgcc ggataaagca ccaacaccat ctcgccgcgc gattggttgc
      661 cgtcggcaga caatgccgtc tgaatttccc caaccgtgcc gcttaagaac gtttcaaacg
      721 ttttcgtaat ttcgcgcgcc agcattaatc ggcgttcggg gaacagttcc gccatatcgg
      781 caagcgtcgc accgatgcgg tgcggcgttt caaacatgac gataggaaac gccgcccgca
      841 cccatttggc aaacagtttc ctgcgttctc ccgatttcgg cggtacaaaa ccgttgaaat
      901 aaaaatcgga tccttccaca ccggccacgc tcaaagccgc catcaccgcg cttgcgccca
      961 cgacgggaac gactttaaac ccggcctcac gcacgcggcg ggcgagtttc gcgcccgggt
     1021 cgcacacggc cggcgtaccc gcatcggaaa cctgtgccac aaccatgccg tctgaaagat
     1081 agccgacaat cttgtccgcc atctgccgtt cgttgtgttc gcgcacactg acgagtttgc
     1141 cctgaatgcc gtacgcgctc aaaagctgtg cggtaacgcg cgtgtcttcg gcacagatga
     1201 tgtccgcctt ttgcaatacc gccaaagcgc gcagggtaat gtccgccaaa ttgccgatgg
     1261 gcgtggcaac cacgtataat gtccctccga cgacgctgtc ggaggctttc tgcaaatgtt
     1321 tctgaaacat aagaatgccg tctgaaaaac aaacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcacg ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgtcgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgatgcgg tacttatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaatgacg
     1861 gcaaaatcct ggcctgcggc aacggcggtt cggctgccga cgcgcaacac ttcgccgccg
     1921 aaatgaccgg ccgttttgaa aaagaacgca tggaactcgc cgctgtcgcg ctgacaacag
     1981 acacttccgc gctgacagcc atcggcaacg actacggttt cgaccacgta ttcagcaaac
     2041 aggtgcgcgc gctcggacgt gcaggcgatg tattggtcgg catttccacc tccggcaatt
     2101 ccgccaacgt catcgaagcc gtcaaagccg cacacgaacg cgatatgcac gtcatcgcct
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttttgctca
     2221 acgttcccca tccgcgcacc gcccgtattc aagaaaacca catcctgctg atacacgcca
     2281 tgtgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagca cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg aagcgccgcc gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgt tcctatctgc
     2581 gccaaaacaa ccaaaccaaa ggctacacgc cccaaatctc cgtcgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgaaaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtgt acaactatat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgccgtattg
     3121 ctcatctgta tcgccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcgaca aaacccttca agccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacgc ccaaagaaaa ccataccaaa ccggacaccc cgaaaaacac gccgcccaaa
     3601 ccccataaag aaattctcga caacctcttc tgacccggca cggcaggcac acccgcaatc
     3661 caaggaagca ttatgaacgg catcatcatc aaaacccccg aagaaatcga aaaaatgcgc
     3721 gagctgggca aactcgtcgc cgaagccctc gactacatcg gacaattcgt caaacccggc
     3781 gtaaccaccg acgaaatcga caaactcgtt tacgactacc acgtcaacgt ccaaggcggc
     3841 tatcccgccc ccctgcacta cggcaacccg ccctacccca aatcctgctg cacctccgtc
     3901 aaccacgtca tctgccacgg cattcccgac gacaagccgc tcaaagaagg cgacattatc
     3961 aacatcgacc tcaccatcaa aaaagacggc ttccacggcg actccagccg tatgtttacc
     4021 gtcggcaaag tctcccccat cgcccaacgc ctgatcgacg taacccacgc ctccatgatg
     4081 gcgggcatag aagccgtcaa acccggcgcg acactgggcg acgtaggtta cgcctgccaa
     4141 caggttgccg aaaacgccgg ctattccgtc gtacaggaat tctgcggaca cggcatcggg
     4201 cgcggtttcc acgaagcccc gcaagtgttg cactacggaa aaaaaggaca gggccccgtt
     4261 ctaaaaccgg gtatgatttt taccgtcgaa ccgatgatca accaaggcaa acgccacctg
     4321 cgtatcctca acgacggctg gacggtggtt accaaagacc gctccctctc cgcccaatgg
     4381 gaacacgaag tcttggtgac cgaaaccggc tacgaaatcc tcaccgtcag ccccgcctcc
     4441 ggcaaaccct gaaaccggac gtatccgccc cataaaaaca aacaatgccg tctgaaagaa
     4501 acggcagata tgatatataa tataaaaaca ggcttgaccc ggcacattac gaaaacaaag
     4561 caaatcggaa tttgccccgc aaccagacaa acttaaagga agttttatga aaatatttga
     4621 aaatatagaa gatgttaaag ccatccgtaa aaagaccggg ctgaaccaga tagacttctg
     4681 gggcaaggtc ggcgttaccc agtccggagg atcgcgctac gaaaccggcc gcaaaatgcc
     4741 caaacccgta cgcgaactgc tccgcctcgt ccatatcgaa tgcatcgatt tggcgaaagt
     4801 caacaaaaaa gatatggaaa tcgccgccct gttgaaaaaa caccatcccg acctgtatgc
     4861 cgagttgtcc aaacagacca agtccgaaag aaaaaaacaa agttaaaccg caacctccgg
     4921 atgcccgaca gtttttcatt tccgaaaaac gcaaacaatg ccgtctgaaa caccggacag
     4981 gtcgccgtat cccgcctgcc gcccctgcct caaaccgccg aaccgcccga acccgccttt
     5041 ttacaaactt tatccaattt cctgtttatt tcgggatacg ccgacattag aatgtcaaac
     5101 agctcgaaac gggcaaactc cacatccatc caaaggaata aaaatgaaac ttctgaccac
     5161 cgcaatcctg tcttccgcaa tcgcgctcag cagtatggct gccgccgctg gcacggacaa
     5221 ccccactgtt gcaaaaaaaa ccgtcagcta cgtctgccag caaggtaaaa aagtcaaagt
     5281 aacctacggc ttcaacaaac agggtctgac cacatacgct tccgccgtca tcaacggcaa
     5341 acgcgtgcaa atgcctgtca atttggacaa atccgacaat gtggaaacat tctacggcaa
     5401 agaaggcggt tatgttttgg gtaccggcgt gatggatggc aaatcctacc gcaaacagcc
     5461 cattatgatt accgcacctg acaaccaaat cgtcttcaaa gactgttccc cacgttaatc
     5521 aggcaacaaa aaacagcgtt ttcagaaatg aaaacgctgt ttttttgacc gttccattat
     5581 tcacaaaagg gaaaaaacga ttacctgccc cgtgtatcaa aacctgccct gccggatgaa
     5641 gggcataacc ggcagggacg gcgtcaacac catatggggg tacggctttt cttgaaagat
     5701 tcggcttaaa tatccaatac tttcgcggta taggcgataa tttcatccgc cctttcaggg
     5761 ttttcgttca acttgatgcc gtaacccggt accagctctt tcagacggtc ttcccaagac
     5821 ggggcgcgct cggggaagca ttggtgcatc agccggatca tcagcggcac agcggtcgat
     5881 gcgcccggcg acgcgcccag caatgcggcg agtgagccgt cggcgtgggc gacaatctcc
     5941 gtaccaaact ggagcacgcc gcctttttcg gagtcttttt taatgatttg gacgcgttgc
     6001 cctgcggtga tgagttccca gtcgtcgggg tttgcctcgg ggtagtattc cagcagggag
     6061 gcgaagcgtt cttctttggt tttacgcaat tcgcccagca ggtatttggt cagcggcata
     6121 ttcgcccagc cggcgcacag cataggatag aggttgtcca tatggatgga cagcggcaaa
     6181 tccataagcg agccttgctt gaggaagttg gaacggaagc ctgcgtaagg gccgaacata
     6241 aggtggcgtt tgccgtccac gttgcgtgtg tcgaggtgcg ggacggacat cggcggcgcg
     6301 ccgacggaag cctgcccgta cactttggcg ttgtgttgtt cggcggtttc ggggttgctg
     6361 ttgcggaaga acaggccgga cacggggaag ccgccgtagc ctttgccttc ggggatgccg
     6421 gatttttgca gcagggtcag cgcgccgccg cccgcgccga ggaagaggaa gcgggtacgg
     6481 agggtgagct gcccgtcggg gttgcgggta tcggcggttt tgagcaccca cgcgccgtcg
     6541 gattcgcgtt tgatgtcttc gacgtggcgg ttgaactcgg tttttacgcc cttgccctgc
     6601 aaatatttca ccatttggcg cgtcagccgt ccgaaatcga catccgtacc ttcggcggag
     6661 tagttggcgg cgacgggttg gttttcgtcc cggccgcgca tcatcagcgg agcccaatcg
     6721 gaaattttgt tccgatcggt ggaaaattcc atattttcaa aaagtttttg ggttttaaac
     6781 gcgtcataac gtttttgaag ataagaacaa tggtcttcat tcatcaccaa agacatatgc
     6841 ggcacggcat tgatgaagga attgtcttcc aacttgcctt ccgcgaccag cgtcgcccaa
     6901 aactggcggc tgacatgaaa ctgttcggca atattgaggg cgcgcgccgg atcgataatc
     6961 ccatttgcac ccaacggcgc atagttcaat tcgcacagcg cggaatgccc cgtgccggcg
     7021 ttgttccacg cgtttgacga ttccaacgcc acatcttcca agcgttcaat cagggtgatt
     7081 tcccaagacg gttcgagttc tttgagcaaa acgcccaaag tcgcgctcat aatgccgccg
     7141 cccaccaaga caacgtctgt cgcttcagcc atggtttact cctaaaaaac aggcatcttc
     7201 tgcccttatg gttatttgcc gtactacaaa cgcctgaatc gcaaaagcag ggaaaaccgg
     7261 caatggtgtg tgtccgagta tgctgtttcg gggttggaat gcgttgcaag catggcttcc
     7321 gacaccgctt caggggcttg taatatgtta tcgtgaatgt agtggatttt actgggaaat
     7381 gcaaagtttt tctgtcgccc gccaagtcgg gaaactgcga aatgaaaaat aaaaatagtt
     7441 atttatctat atatatcaaa tttttaatag ataaaaaatc aaaattgttt atatattaat
     7501 ttttaaaaga ttgtcagcat attgcgt
//
LOCUS       DAWIVB010000101         7501 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWIVB010000101 DAWIVB010000000
VERSION     DAWIVB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.101"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            125..1069
                     /gene="tehA"
                     /locus_tag="WIX60_001421"
     CDS             125..1069
                     /gene="tehA"
                     /locus_tag="WIX60_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEZ5305680.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRS"
     gene            complement(1142..1825)
                     /locus_tag="WIX60_001422"
     CDS             complement(1142..1825)
                     /locus_tag="WIX60_001422"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ5305681.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1973..4276
                     /gene="parC"
                     /locus_tag="WIX60_001423"
     CDS             1973..4276
                     /gene="parC"
                     /locus_tag="WIX60_001423"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ5305682.1"
                     /translation="MNTQAHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPPHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLSDENEKRKLIVKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDCLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SSGYGFITKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSELEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4296..5813
                     /locus_tag="WIX60_001424"
     CDS             4296..5813
                     /locus_tag="WIX60_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ5305683.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYSSYAAILLIFNAIADGDIGKYPLISDAR
                     TASATFILVAASYLSAIFTSLSVKYIDRAGKLAYDSHIAYHRIKGLSQTVLERVQEAV
                     VVINAEGLAVLFNRKAKDLFPALEIGRRAGLSDSAAELWDQASPHTFEYVLGTPGLNA
                     GIRAVPVNKGSDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNICRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAADCIRPDIQGGSPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            5865..6332
                     /locus_tag="WIX60_001425"
     CDS             5865..6332
                     /locus_tag="WIX60_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ5305684.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            6436..7188
                     /locus_tag="WIX60_001426"
     CDS             6436..7188
                     /locus_tag="WIX60_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ5305685.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQNTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQDPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1674 a         2260 c         1978 g         1589 t
ORIGIN      
        1 tcacttggtg cttcagcacc ttagagaatc gttctcttcg agctaaggcg aggcaacgtc
       61 gtactggttt ttgttaattc actatatctg tgcgttacga cggcatgccg tctgaagggt
      121 gtttatgtct gcatctaaga aatttccgat tcctttgagc tatttcagca tcgcgctggg
      181 cttgtttgcc ttggggctgt cgtggcgtta cggcgcgtct gtcgggctgc tgcccgcctt
      241 ggccgccgaa tcgctgcttg cggcggcttc ggtcgtctgg ctcttgctgg tggcggcata
      301 cctgatcaaa atgtttgcgt accgaaacga ttttttgtct gatttacgcg acttggtgca
      361 atgctgcttc atcagcgcga ttccgattac cgctatgctg gagggactcg cgctgaagcc
      421 ctatcaggca ggcgcggcgg cagtcctgat ttatgtcggc gttgccggac agttggcttt
      481 ttcgatgtat cgggcggccg gtctgtggcg cggcctgcat tccttggagg cgacgacgcc
      541 gattatttat ctgcctacgg ttgcgacaaa ctttgtcagc gcgtcatctc tggcggcgtt
      601 ggggcatcat gattatgcag ctttgttttt cggcgcgggt atgttttcct ggctgagctt
      661 ggaagcctcc atcttgggca ggctgcgcac ggcggcaccg gtcggcacgg cggcgcgcgg
      721 cgtggtcggc atccagcttg cgcccgcctt tgtcggctgc ggcgcgtatt ttgccgtcgg
      781 cggtaaagtc gacggttttg cgttggcatt aatcggctac ggctgcctgc agcttttgtt
      841 cttgctgcgc ctgacccgct ggttttggga aggtggtttt acgatgagct tttggggatt
      901 ttcattcggt ttcgcggcaa tggcaggatg cggtctgcat ctggcggctt ccggcgtatt
      961 gtcgggcttg gggctgacgc ttgccaccgc cggatcggca ggcgtggcgc tgctgcttgt
     1021 cggtacgctg caccggatag cgacggggcg tttcttggta cgcagctgat gcgttttgcc
     1081 gccttgtcaa aaatgccgtc tgaaacgctg ggattcagac ggcatttttt atttcacacc
     1141 cttacaggta gaatttttcg atgactttca aattgtcgtc caatttgtac accaacggct
     1201 gaccggtcgg gatttccaag cccataatgt cttcgtcgga aatgccctcg atgtgttttg
     1261 ccagcgcgcg cagggagttg ccgtgcgccg ccaccaagac gcgtttgccg ctcaaaatcg
     1321 cgggggcgat ttggtcttcc caaaacggca atacgcgctc cagcgttact ttcaggtttt
     1381 cgccgtcggg tacgacatcg gcaggcagat gggcatagcg gcggtctttg tgtgcggaaa
     1441 actcatcgtc tttgtccaaa agcggcggca gggtgtcgta gctgcgccgc cagatgcgga
     1501 cttgctcgtc gccgtattgt tcggcggttt gttttttgtc caggccttgc agttggccgt
     1561 agtggcgttc gttcagccgc cacgttttga tttgcggtac gaacagttgg tcggattctt
     1621 ccaaaacgat gttgcaggtc ttaatcgcgc gggtcaggac ggatgtgaag gcgatgtcga
     1681 actcatagcc gttttctttc agtttcttgc cggcggcggc agcctcggca agcccctgct
     1741 cgctcagctt cacgtcgcgc cagcctgtaa acaggttttt cgcgttccat tcgctttgtc
     1801 cgtggcggat aaataccagt tccatatcgt ctccaatgtg tgaaagtggg aaagccttat
     1861 ttataacata ttttcacatt tcccgtattt gattcagatt cagacacgcg cccactatgg
     1921 tttgccgttt tgatttacaa taatgtcctt tgctttacat tccgcataca caatgaatac
     1981 gcaagcgcac gccccacata ccgattccaa tacgctgatg ctcggccgat acgccgaacg
     2041 cgcctatctc gaatacgcca tgagcgtggt caaaggccgc gcgctgcctg aagtttcaga
     2101 cggccagaag cccgtgcagc ggcgcatttt gtttgccatg cgcgatatgg gtttgacggc
     2161 gggggcgaag ccggtgaaat cggcgcgcgt ggtcggcgag attttgggta aataccaccc
     2221 gcacggcgac agttccgcct atgaggcgat ggtgcggatg gcgcaggatt ttaccttgcg
     2281 ctatccctta atcgacggca tcggcaactt cggctcgcgc gacggcgacg gggcggcggc
     2341 gatgcgttac accgaagcgc ggctgacgcc gattgcggaa ttgctgttgt ccgaaatcaa
     2401 tcaggggacg gtggattttg tgccgaacta cgacggcgcg tttgacgaac cgctgcacct
     2461 gcccgcccgc ctgcctatgg tgttgctcaa cggcgcgtca ggcattgcgg tgggcatggc
     2521 gaccgagatt ccgccgcaca atttgaacga agtgacgcag gcggcgattg cgttgttgaa
     2581 aaagccgacg ctggaaaccg ccgacctgat gcaatatatt cctgcccccg attttgccgg
     2641 cggcggtcaa atcatcacgc cggcggacga attgcgccgg atttatgaaa ccggcaaggg
     2701 cagcgtgcgc gtgcgtgcgc gttatgaaat cgaaaaattg gcgcgcggac agtggcgcgt
     2761 catcgtaacc gagctgccgc cgaacgccaa ttccgccaaa atccttgccg aaatcgaaga
     2821 gcaaaccaac ccgaaaccga aagcgggtaa gaaacagctc aaccaagacc agctcaatac
     2881 caaaaagctg atgctggatt taatcgaccg cgtgcgcgac gagtccgacg gcgaacatcc
     2941 cgtgcgactg gtattcgagc cgaaatccag ccgcatcgat accgatacct tcatcaacac
     3001 gctgatggcg caaacttcgc tggaaggcaa tgtgtcgatg aacttggtga tgatgggttt
     3061 ggacaaccgc cccgcgcaga aaaacctgaa aacgattttg caggaatggc tggatttccg
     3121 caccgtaacc gtaacacgcc gtctgaaatt ccgtttgaac caagtggaaa aacggctgca
     3181 catcctcgaa ggccgtctga aagtctttct gcacatcgac gaagtgatta aagtcatccg
     3241 cgaatcagac gacccgaaag ccgatttgat ggcggcgttc gggctgaccg aaatccaagc
     3301 cgaagacatt ttggaaatcc gcctgcgcca gttggcgcgt ttggagggtt tcaaactcga
     3361 aaaagaattg aacgagttgc gcgaggaaca aggccgtctg aacatccttt tgagcgacga
     3421 aaacgaaaaa cgcaagctga ttgtcaaaga gatgcaggcg gatatgaaac aatacggcga
     3481 cgcgcgacgc acgctggtgg aagaggccgg acgcgccgtg ctgacgcaga ccaccgccga
     3541 cgaacccatc acgctgatcc tgtcggaaaa aggctggata cgcagccgcg ccggacacaa
     3601 tctcgatttg agccaaaccg cgttcaaaga aggcgactgc ctcaaacaaa ccctcgaagg
     3661 cagaacggtt ttacccgtcg tcatcctcga ttcatcgggc agaacctaca cgctcgatgc
     3721 cgccgaaatc cccggagggc gcggcgacgg cgtaccggtt tcctccttaa tcgagctgca
     3781 aaacggcgcg aaacccgttg cgatgttgac aggattgccg gaacaacatt atttattatc
     3841 aagcagcagc ggctatggct tcatcaccaa gctgggcgat atggtcgggc gcgtgaaagc
     3901 gggcaaagtg gtgatgaccg cagacagcgg cgaaaccgtt ttgccgccgg ttgccgtcta
     3961 tgcctcctcg ttcatcaacc ccgactgcaa aatcattgcc gccaccagtc aaaaccgcgc
     4021 cctcgccttc cccatcggcg aattgaaaat tatggcgaaa ggcaaagggc tgcaaatcat
     4081 cggattaaac gccggcgaat cgatgacgca taccgccgtt tcttccgagc tggaaatcct
     4141 gattgaaagc gaaggcaggc gcggcgcggc gcacaaagac cgcatcccca tctccctgct
     4201 tgaggcaaaa cgcggcaaaa aaggcagact attgcccata tcgggcagcc tgaaacagct
     4261 ttcttcccct aaataaaccc ggttccgcac atattatggt gatttccaac ccccgcgaac
     4321 ttgaaaaact caaagaccgg attcccaatc tgatcaacat catccgcgtc gccatcgttt
     4381 ttccgctgat gattatgcac atcctcgggc tggaaaccgg cagccgtgcg aacctgcacg
     4441 cttcgtggac ggcgtgggcg ttttatgttt ggctcgccat tgcctgctgg ctgattttct
     4501 tttccattat ccatccgcat tggcaatggc agtcgctgaa aatgccgcgt ttcagcgcgg
     4561 tagcggacat cacgatgatc ggcgtgctga cctacctgtt cggcggcatc gattccggct
     4621 tcggcatcct gatcctgccc ttcgtcgtct gctcctgcct gctcagctac gggcgctacc
     4681 ccctgctcta ttccagctac gccgccatcc tgctgatatt caacgccatt gccgacggcg
     4741 atatcggcaa atacccgctc atatcggatg cccgaaccgc ctcggcaacc ttcatccttg
     4801 tcgccgcctc ctatctttcc gccatcttca cctcactgtc ggtcaaatac atcgaccgtg
     4861 ccggaaaact cgcctacgac agccatatcg cctaccaccg catcaaaggc ttgagccaaa
     4921 ccgtactcga acgcgttcag gaagctgtcg tcgtcatcaa tgccgaaggg ctggcggtgc
     4981 tgttcaaccg gaaggcgaaa gaccttttcc ccgcgctcga aatcggacgg cgcgccggtc
     5041 tgtccgattc tgccgccgaa ctgtgggatc aagcctctcc gcacactttc gaatacgtcc
     5101 tcggcacacc cggcctgaac gccggcatcc gcgccgttcc ggtcaacaaa gggtcggaca
     5161 agctgctcat cctctacatc cgcccgcaaa gcgaaattca ggcagaagcc ctgtccgtca
     5221 aacttgccgc gctcggacaa ctgaccgcca acctcgccca cgaaatccgc aacccgatgt
     5281 ccgccatccg ccacgccaac gacctgctgc gcgaaaatat ggaagcgggg gcggcagatc
     5341 cgttcaacgc caaattgtgc aaaatcatcg acggcaacat ctgccgcatc gacaaaatgc
     5401 tcgaagacat ttcctcgctc aacaagcgca acaaaaccga acgcgaaacc atcggcctga
     5461 taccgttttg ggaagaattc aaacaagagt tcctgctcgg ccatcccgat gccgccgact
     5521 gcatccgtcc ggacattcaa ggcggcagcc cgaccgccta tttcgatccc gcccacctgc
     5581 ggcaaattat gtggaacctc gccaacaacg cgtggcggca cagccgcaaa cagcccggct
     5641 cgatttccgt caccatccgc cccgcgcaaa aaaacaccgt ctgtatcctc tttgccgacc
     5701 gcccgaagtg caggaacacc tgttcgaacc cttttacacc acggcggaaa acggcaccgg
     5761 cctcgggctg tatgtcgccc gcgaactggc gcacgccaat ttcggcgatt tgacctacct
     5821 accggaagcc aaatgtttcg aactcacatt accggaaaaa accaatgact gaactgcaac
     5881 accccgtcct cgtcgtcgat gacgaaaccg acattctcga cctgatggaa atgaccctga
     5941 tgaaaatggg cttgcgcgtc cataccgcgt caggcgttgc cgaagccaaa aacaagctcg
     6001 acagccaacg ctattcgctc gtcctgaccg atatgcgtat gccggacggc tcggggctgg
     6061 aagtcgtcca acacatcaac agccgcctgc tcgatacgcc ggttgccgtc atcaccgcct
     6121 tcggcaacgc cgatcaggca caggaagcgt tgcgttgcgg cgcgttcgac cccgatacca
     6181 tgcagataca ggactatctc gaccaaatcg aacgcgacat catcgaacaa accctcaaac
     6241 aaaccgaagg caaccgcacg caggccgcca aacgcttggg catcagcttc cgttccatgc
     6301 gctaccgtat ggaacgcctc aacatcggct gacgacaaaa cggcatccgc accatctccg
     6361 cccacccgaa aaaatgccgt ctgaaacggc acgggaaagc gggttcgccc cacgcccgaa
     6421 cggacacaaa acaccatgac cgacatcctt attgacaaca ccgccaccga aaccgtccgc
     6481 accctgatac gggcattccc ccttgtgccc gtttcccaac cgcccgaaca aggcagttac
     6541 ctccttgccg aacacgatac cgtcagcctc aggcttgtcg gggaaaaaag cagcgtcatc
     6601 gtcgattttg cctccggcgc ggcacaatac cggcgcacaa aaggcggggg cgaactcatc
     6661 gccaaagccg tcaaccacac cgcgcacccc accgtttggg acgcaaccgc aggattgggg
     6721 cgcgacagct tcgtcctcgc ctcgctcggg ctggccgtta ccgccttcga gcaacatccc
     6781 gccgtcgcct gcctgctttc agacggcatc cgccgcgccc tcctcaatcc cgaaacgcaa
     6841 aacaccgccg cgcacatcaa cctccatttc ggcaacgccg ccgaacaaat gcccgcactt
     6901 gtccaaacac aaggcaaacc cgacatcgtc tatctcgacc ccatgtatcc cgaacgccgc
     6961 aaaagtgccg ccgttaaaaa agaaatgacc tacttccacc ggctcgtcgg cgaagcgcaa
     7021 gatgaagcgg cactcctgca taccgcacgc caaacagcaa aaaaacgcgt cgtcgtcaaa
     7081 cgcccccgcc tcggcgaaca ccttgccgga caagaccctg cctaccaata cacaggcaaa
     7141 agcacccgct tcgacgttta cctgccctac gggacggaca agggataacg cccataaaac
     7201 aagacaccga aaaatttgcc gttcttatgc aaacgagaaa ccggtttttg cgtttcgact
     7261 gttttggata agtcatcaca ccttaaagtt tgtcattccc acagaagtgg gaatccgatt
     7321 cattcagttt tatagtggtt taaatttaaa ccactatagt tgttttcgag tttcaggcaa
     7381 cttccaaacc gtcattccca cggaagtggg aatctagaaa tgaaaggcaa caggaattta
     7441 tcgtaaatga ctgaaaccga acggactaga ttcccgccta cgcgggaatg acggggcggg
     7501 c
//
LOCUS       DAWIVB010000102         7469 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWIVB010000102 DAWIVB010000000
VERSION     DAWIVB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.102"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(16..954)
                     /locus_tag="WIX60_001427"
     CDS             complement(16..954)
                     /locus_tag="WIX60_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305686.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGMLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            1156..2268
                     /gene="nadA"
                     /locus_tag="WIX60_001428"
     CDS             1156..2268
                     /gene="nadA"
                     /locus_tag="WIX60_001428"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ5305687.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            2426..2755
                     /locus_tag="WIX60_001429"
     CDS             2426..2755
                     /locus_tag="WIX60_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEZ5305688.1"
                     /translation="MHWLFLTVAILSEVCGSSMLKLSGGFSKLWPSIGVVVSFSVCFW
                     ALSMTLKTMPLATAYAIWAGVGLVLTALVSVVFFGEKADFIGIVSIGLILLGVVLLNT
                     MSHMSGH"
     gene            2804..4312
                     /gene="nadB"
                     /locus_tag="WIX60_001430"
     CDS             2804..4312
                     /gene="nadB"
                     /locus_tag="WIX60_001430"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ5305689.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRAILSQGKPAIEWLLAQGVAFDR
                     NHNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VGNLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLCAQA
                     IADGQAFQAEPFQRPSESLSAEAGIFSDDLQNTFSRPVLQTFNQRHLGILRNDTGLRR
                     AIAQLQLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(4490..5155)
                     /gene="pgmB"
                     /locus_tag="WIX60_001431"
     CDS             complement(4490..5155)
                     /gene="pgmB"
                     /locus_tag="WIX60_001431"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ5305690.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGISIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVSEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LRANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVDA
                     DIRQCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSGTAELTYAYLQSVWEQ
                     SGR"
     gene            complement(5168..7426)
                     /locus_tag="WIX60_001432"
     CDS             complement(5168..7426)
                     /locus_tag="WIX60_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ5305691.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINAFNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFESEVGSTPETFEKRVIVTTSRDYQSLEAVKAAGRAL
                     SEKIAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRADELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFSDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGESLDLQVYGKDI
                     TLDGSHTVALEK"
BASE COUNT         1532 a         2039 c         2196 g         1702 t
ORIGIN      
        1 tcgtctgaaa acgggctaac ggctgcccag cggcagtccg atgtccgaaa tcagcctgtc
       61 gggcaggacg tcgtcgcgga agcggcaaag ctgcgcggga cggcctttgc tgcccgatac
      121 gccggtatcc gacggctcga tgaggttttg ctgctgaatc tggcggcgga agttttgctt
      181 gtgcagcaat ctgccgctga tggcttcgac gctgttttgc agttgcagca gcgtgaattc
      241 gggcggcatc agttcaaaaa tcacggggcg gtatttgatt ttggcgcgca ggcgagacag
      301 ggcggtcgcc agtacgcggc ggtggtcgtg gcgcatgggc tgccccgtaa gcgcgaagtc
      361 gaagtttgcc tgcggctcgg cggcttccgc tatcaggccg ctttcataca gcatttcata
      421 gcgttgcaaa acgtattctt ccgaccagtt ttccggttcg accccccagc acaaatgaat
      481 gcgcttgagc cgcttttggc gcacttcctc cgtgtccgcc gagtttgccc aaatgcgcag
      541 gcggccgacg acggcgtcgc gctgcccgcc gtcggtgcgc aagtcttccc acgggaaata
      601 gccgtagcag tcctgccatt tcgcatccgg gtgcaggatg ctgtcggctg cctcgcgcac
      661 cagccccaaa tagctgacgt acagcacggg catgccgtgt tcgttgcggc ggtgggtatc
      721 gacaaaggtg taaagctgtt ccacatagcc cataggctgc gaagtctgct tggcgaccca
      781 cagttttacg cctgcctgca aggaattgcg caggggggag agcgggccgt tgggcaggag
      841 catgccttgg gcgacggtca atacccgcag gccgccgtcg gtaacggcaa tcaatacggg
      901 aaccagctcg acgatgcttt gcggcggggc ttcggcttcg gggtaggcat ccatgctcgg
      961 ctttctgcgg gtgggaaagg ctttattgta aaccaaagcg gtttgtcgga acgaagcggg
     1021 tcggttttac ggtattgcaa acctgttttc agacgacctt gcatattata ctcaagttga
     1081 gcataatgta aaaacagttt gaaaaacaga cggcaaacct tcatgccgtc tgaaaaacag
     1141 ctacaaggaa acatcatgca aaccgccgcc cgccgctcgt tcgactacga tatgccactc
     1201 atccaaacgc cgacttccgc ctgccaaatc cgtcaggcgt gggcgaaggt tgccgacacg
     1261 cccgaccgcg agacggcaga tcgtctgaaa gacgaaatca aggctttgct gaaggagaaa
     1321 aacgcggtct tggtggcgca ttattacgtt gatccgctga ttcaggattt ggctttggag
     1381 acgggcggat gcgtgggcga ttcgctggaa atggcgcgct tcggtgcgga acacgaagcc
     1441 ggtacgctgg tggtggctgg tgtgcgcttc atgggcgaga gcgcgaaaat cctctgccct
     1501 gaaaaaacgg tgctgatgcc tgatttggag gcggaatgtt ctttggattt gggttgcccg
     1561 gaagaagcgt tttcagcgtt ttgcgaccaa caccccgacc gcacggtggt ggtgtacgcc
     1621 aacacttccg ccgccgtgaa agcgcgtgcc gattgggtgg taacgtcttc ggtggcgtta
     1681 gaaatcgtat cgtatttgaa atcacgcggc gagaagctga tttggggacc cgaccgccac
     1741 ctcggcgact acatctgccg cgaaacgggc gcggatatgc tgttgtggca gggttcgtgc
     1801 atcgttcaca acgaattcaa agggcaagag ctggcggcgt tgaaggcgga acaccccgaa
     1861 gcggtggtgc tggttcatcc tgaatcgccg caaagcgtca tcgaactggg cgacgtggtc
     1921 ggctcgacca gcaaactgct caaagccgcc gtatcgcgtc ctgaaaaaaa attcatcgtg
     1981 gcgaccgatt tgggcatcct gcacgaaatg caaaagcagg cgcccgacaa acaatttatc
     2041 gccgcgccga cggcgggcaa cggtggaagc tgtaaaagct gcgcgttctg cccgtggatg
     2101 gcgatgaatt cgctgggcgg catcaaatac gccctgacaa gcggacgtaa cgaaatcctg
     2161 ttggacagga agctgggcga agccgccaaa ctgcctttgc agcgtatgct cgactttgcg
     2221 gcagggctga agaaaaaaga tgtgttcaac ggcatgggcc ccgcctgatt tgtcgttcgt
     2281 aatgggtgca gcctgaaaat accgcccgcc tgctttccca agacgggatt tgcacacaaa
     2341 aaatctttaa caacaagact gtttttttat tttattgcat tgatataatt atactttctt
     2401 tcatttatca ggtaaaaaca tgcaaatgca ctggctcttt ctgactgtag caattttaag
     2461 cgaagtctgc ggttcttcca tgctcaaact gagtggcggg tttagcaaac tgtggccttc
     2521 tattggcgtg gtagtcagct tttcggtgtg tttttgggcc ttgtctatga cactgaaaac
     2581 catgccgctg gctacagcat acgccatttg ggcaggcgtg ggactggttt taacggcttt
     2641 agtcagcgtg gtgtttttcg gtgagaaagc tgatttcatt gggattgtca gcatcggttt
     2701 gattttgctg ggcgtggtgc ttctcaatac catgtcgcat atgtcggggc attgattaat
     2761 ccactatacc gtttcagacg gcctctcaaa caaggacaac accatgcaaa ccgattgcga
     2821 cgtattgatt gccggaaacg ggctggcggc actgacgctc gccctgtcgc tgcctgaatc
     2881 gttccgcatc gttattttgt gcaaaaaccg gctggacgac accgccagcc gtcatgcgca
     2941 aggcgggatt gcggcggcgt ggtcgggaga ggacgacatc gaaaaacacg ttgccgatac
     3001 tttggaagcg ggcgcgggtt tgtgtgatga agccgccgtc cgcgccatcc tgtcgcaggg
     3061 caaaccggca atcgaatggc tgctggcgca gggcgtggcg ttcgaccgga atcataacgg
     3121 cctgcacctg acgcgtgaag gcgggcatac ctgccgacga atcgcccacg tcgccgacta
     3181 cacgggcgaa gccgtcatgc agagcctgat tgcccaaata cgccgccgcc cgaacatccg
     3241 cgtttgcgag cggcagatgg cgttggacat tcaaaccgaa tcaggcgcgg cgtgcggact
     3301 gaccgtcctc gactgccgaa cacaagaaac ctaccgcatc cgcgcccgcc ataccgtact
     3361 cgcaggcggc ggcttgggac agatttacgc cgccaccacc acgccgcccg aatgcacggg
     3421 cgacgccatc gccatggcga tacgcgcagg ctgcgcagtt ggaaacctcg aatttatcca
     3481 attccacccc acaggcttgg caagaccgtc tgaaaacgga cgcaccttcc tcatttccga
     3541 agccgtgcgc ggcgaaggcg gcatcctgac caaccaagcg ggcgaacgat ttatgccgca
     3601 ttacgaccgc cgcgccgaac tcgcaccgcg cgacatcgtt gcccgcgcca tcgcagccga
     3661 aatcgccaaa caaacgcaag acttcgtctc gctcgacatc agccatcaac ccgcagcgtt
     3721 cgtccgccgg catttcccat ccatccatcg acactgttta tcccaatgcg gcctagacat
     3781 cacgcgccaa gccatccccg tccgccccgt gcaacactac acctgcggcg gcatccaaac
     3841 cgacccctgc ggcagaacct ccctgccgca gctctacgcc ttgggcgaaa ccgcctgcac
     3901 tgggctgcac ggagccaacc gccttgccag caactccctg ctcgaatgcg tcgtgaccgc
     3961 aaggctctgc gcccaagcca tcgcagacgg acaagcattc caagccgaac cattccaaag
     4021 gccgtctgaa agcctctccg ccgaagcagg catcttttca gacgaccttc aaaacacatt
     4081 cagccgcccc gtcctgcaaa cgttcaacca acgccatcta ggcatcctac gcaacgatac
     4141 cggcctgcgc cgcgccatcg cccaactgca gctttggaag caaaaccaag ccgaaccgca
     4201 caccgcgtcc gaatacgaaa accgcaacct gctcgaatgc agcctcgccg tcgcccaagc
     4261 cgcatacagg cggagacaga acatcggcgc gcattttaat agtgattgtt aaggttggct
     4321 ggttagaaaa aaaatcgttc aaataaattt gaatagcatg gttaagagat tggcatttag
     4381 tttttagggt tgcgtaagtt gggttatcat tctgttctaa aagcaaaaag gccgtctgaa
     4441 acactgttca gacggtcttt tgcttccttg acactttatc tgacgcgttt tacctgcccg
     4501 actgttccca cacgctttgc aggtaggcgt aggtcagctc ggcggtgccg gagaccagcg
     4561 cgatgtcgct gcccaagtct tcggctttgc ccacgccgat gggcaaggcg ccggcggctt
     4621 tgatggcggc gacgccggcg gcggcgtctt caatgccgat gcattggcgg atgtccgcat
     4681 ctacgccctc ggctgctgcg aggaagatgt cgggggcggg tttggaatgt gcgacggcgg
     4741 cagggtcggc aatggcgtcg aagaagtggg tcagccccat gcgttccagc aggaacgggc
     4801 cgtttttact ggcggacgca agggcgattt ttttgccgtt tgccctcaat gcttccagca
     4861 ggggcaaaat gccgggatac acgtcttcgg gtttgactgc ctgaatcatc tcgacgtagt
     4921 tgtcgttttt acggcgggtc agttcggcga actcggcttc gctgacggtt ttgccgccgt
     4981 gcgcgaggat gcgtttgagc gaatcgtcgc gcgacacgcc tttgagctgc tcgttaaact
     5041 tgcggtcaat gctgatgccc agttcttcgg cgagcttttt ccatgcgcgg tagtggtatt
     5101 cggcggtgtc ggtgatgacg ccgtcgaggt caaataggac tgcagtgaaa gtcattttgc
     5161 gccctcctta tttttccaac gcaacggtgt ggctgccgtc gagcgtgatg tctttgccgt
     5221 acacctgcaa atcgagcgac tcgcctttga gcagagtgaa gacgacgttt tctttgccga
     5281 cggcgacttt aatcagacgg ccgcggtagt tgatgtggaa ggcgtagcct gtccacgcac
     5341 tcggcaggaa cggtgcgaag ctgagtttgc cgccccaggt tttcatttgg gcgaaacctt
     5401 ggacgatggc gagccacgag ccggtcatgg aggtgatgtg caggccgtct tcggtgtcgt
     5461 tgttgtagtt gtccaagtcc aggcgggcgg tgcgctggta catttccacg gctttttctt
     5521 ctttgcccag ttcggcggcg agaatagagt gaatacaggg cgacagcgag ctttcatgca
     5581 cggtcatcgg ttcgtagaag tcgaagttgc ggcgtttttc gtcgatattg aaacggtcgc
     5641 tgaagaagta gatgccttgc aatacgtccg cctgtttgat aaagggcgaa cgcaggattt
     5701 tgtcccacga ccatttttgg ttgagcggca aatcgtcggg cgaaagcgcg gacacggggc
     5761 ggatgtcttt gtcgaggaag ccgtcgtgct gcacgaatac gccgagttct tcgtcatgcg
     5821 gacggtacat attcgcgctg atgtccgccc atttttccaa ctcgtcggca cgcacgttca
     5881 aatccggacg cgggtatttc gccaaggctt cgcgggtgta gtccaatacc catgcggcga
     5941 gggtgttggt gtaccagttg ttgttgatgt tgttttcgta ttcgttcgga ccggttacgc
     6001 cgtgaatcat gtatttgccg ttgcgtttgg agaagtggac gcggtccgcc cagaagcggg
     6061 acacttcgac caaaacttcc aagccttctt tggcaagata gccctcgtcg ccggtgtagt
     6121 tggtgtagtt gtagatggcg taaggaatcg cgccgttgcg gtggatttcc tcgaaggtga
     6181 tttcccattc gttgtggcac tcgatgcccg taaacgttac catcggatag agtgcgcccg
     6241 ccaagccctg ttcgcgcgcg ttgtgctgcg cctgcggcag ttggttgcgg cggtattgca
     6301 gcaggttgcg ggtaacttcg ggttcggcca gtgcgaggta gagcggtacg gcgtaggctt
     6361 cggtgtccca ataggtcgcg ccgccgtatt tttcgccggt aaagcctttc gggccgatgt
     6421 tcagtcgcgc gtcttcgccg tagtaggtgg agaacagttg gaacaggttg aagcggatgc
     6481 cctgctgcgc ttcgtcgctg ccttcgatga ccacgtcggc gatttcccaa cggtgcagcc
     6541 agcctgcttt gtgcgcgtcc agcaaggttt caaacgcaac gcctgcaatt ttttccgaca
     6601 aggcgcggcc tgcggctttc actgcttcca agctctgata atcgcggctg gtggtaacaa
     6661 tcacgcgttt ttcaaaggtt tcgggtgtgc tgccgacttc ggattcaaaa gaattggaga
     6721 cctgccagtc ggtttggctg ccgccgaggg ctttgaagct gccggcaaag gtttgctcgg
     6781 cgttgacgat gaattgttcc acgccgaagg gattggcgac ggtttgggcg gcaatgtagg
     6841 agagactgtc tgaaacgcct ttgtccaata cctgccagaa tttttcttcg tagttggagt
     6901 cttcgttttt cacgtcggca tcgatgatgg aatcgatgcg gacttggtgg gttttaccgt
     6961 caacggatac ggcttcccag cggatgaccg ccagctcttt ttgtgcgaca gacaggaatt
     7021 tgcacacatt gaaacgcaca ccgaatacgg tgaacgagcg gcgcaacacg ccgtgctgca
     7081 tatcgagttc gacggagaag ccagcaacgt cgtttttcgc caagtccact tcctgcccgt
     7141 cgacaaagat tttgactttg ctgaaattga acgcgttgat ggctttgccg aaatatttgg
     7201 gatagccgtt tttccaccag ccgacgcggg ttttgtcggg gaaccacacg ccggcgatgt
     7261 aggtgcctaa gtggctgtcg gcggaatagg tttcctcaaa gctgccgcgc atacccatat
     7321 agccgttgcc caagctggtc aggctctctt gcagccgttt gtgttctttt tccagttttg
     7381 ccgaacgcag cgtccaaggg ctgatttcca tgattcttgt gtacatttat gaagctcctg
     7441 tttggattga tttgagggaa tggtgaaat
//
LOCUS       DAWIVB010000103         7456 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWIVB010000103 DAWIVB010000000
VERSION     DAWIVB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.103"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            37..2052
                     /locus_tag="WIX60_001433"
     CDS             37..2052
                     /locus_tag="WIX60_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ5305692.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPNETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNATPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     VGSSWTDEYGNPQKYEVCKRWLGELSPYHNLSDGINYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYAPDGGGHTGNGTQRESADEMACVLLFLKEFLG"
     gene            complement(2166..3122)
                     /locus_tag="WIX60_001434"
                     /pseudo
     CDS             complement(2166..3122)
                     /locus_tag="WIX60_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3358..4323
                     /locus_tag="WIX60_001435"
     CDS             3358..4323
                     /locus_tag="WIX60_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5305693.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKAAFEKAEPVAAGKE"
     gene            4813..5136
                     /locus_tag="WIX60_001436"
     CDS             4813..5136
                     /locus_tag="WIX60_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ5305694.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            5250..7427
                     /locus_tag="WIX60_001437"
     CDS             5250..7427
                     /locus_tag="WIX60_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ5305695.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYTRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         1675 a         2256 c         2149 g         1376 t
ORIGIN      
        1 ccactataaa gacctgccca accctcaagg accccgatga aatcctaccc cgacccctac
       61 cgccattttg aaaacctcga ttccgccgaa acgcaaaact tcgctgctga agcgaatgcc
      121 gaaacgcgcg cgcgtttttt agaaaacgac aaggcgcgag cattgtccga cggcattttg
      181 gcgcaattgc aggacacgcg gcagattccg ttttgtcagg aacaccgcgc gcggatgtac
      241 catttccatc aggacgcaga atatccgaag ggcgtgtacc gcgtgtgtac cgcggcgacg
      301 taccgttccg gctatcccga gtggaaaatc ctgttttcag tggcggattt cgacgaattg
      361 cttggcgacg atgtgtattt gggcggcgtg tcgcacttgg tggaacagcc caaccgcgcg
      421 ttgttaacac tgagcaaatc gggcggcgat acggcttata cgctggaagt ggatttggaa
      481 gcaggggaat tggtagaagg cggttttcat tttccggcgg ggaaaaacca tgtgtcgtgg
      541 cgcgatgaaa acagcgtgtg ggtgtgtcct gcttgggacg aacggcagtt gaccgaatcg
      601 ggctatccgc gcgaggtgtg gctggtggag cgcggcaaga gtttcgagga aagcctgccg
      661 gtgtatcaaa tcggcgaaga cgggatgatg gtgaacgcgt ggcgttacct cgatccgcag
      721 ggttcgccga ttgatttgat tgaagcgtcg gacggttttt acaccaaaac ctatttgcag
      781 gtatcggccg aaggcgaggc gaaaccgtta aacctgccca acgattgcga tgtggtcggc
      841 tatctggcgg ggcatctttt gctgacgctg cgcaaggact ggaaccgcgc gaaccaaagc
      901 tatccgagcg gcgcgctggt ggcggtgaag ctgaatcggg gcgaactcgg ggcggcgcag
      961 cttttgtttg cgcccaatga aacgcaggca ttggaaagcg tggaaacgac caagcgtttt
     1021 gtggtggcga gcctgctgga aaacgtacaa ggccgtctga aagcgtggcg ttttaccgac
     1081 ggcaaatggc aggaagccga actgccgcgc ctgccctcgg gcgcgctgga aatgaccgac
     1141 cagccttggg gcggcgacgt ggtttacctt gccgccagcg atttcactac gccgctgacg
     1201 ctgtttgcgc tggatttgaa cgtgatggaa ctgaccgtca tgcgccgcca gccgcagcag
     1261 ttcgattcag acggcatcaa cgtgcagcag ttttggacga cttcagccga cggcgagcgt
     1321 attccttatt tccacgtcgg caaaaacgcc acgcccgaca cgccgacctt ggtttatgcc
     1381 tacggcggtt tcggtgtacc cgaactgccg cattatctgg gcagcatcgg caaatactgg
     1441 ttggaagagg gcaatgcctt tgtattggcg aacatacgcg gcggcggcga gttcggcccg
     1501 cgctggcatc aggcggcgca gggaatcagc aaacacaaaa gcgtcgatga tttgcttgcc
     1561 gtcgtgcgcg atttgtccga acgcggtatc agttcgcccc aacacatcgg cttgcagggc
     1621 ggcagcaacg gcggcctgat taccgccgcc gccttcgtgc gcgaaccgca aagcataggc
     1681 gcgctggtgt gcgaagtgcc gctgaccgat atgatccgtt atccgctgct ctccgtcggt
     1741 tcaagctgga cggacgaata cggcaacccg caaaaatacg aagtctgcaa acgctggctg
     1801 ggcgaattgt cgccgtatca caatctttca gacggcatca attatccgcc tgcgctcatt
     1861 accaccagtc tgagcgacga ccgcgtccat cctgctcacg cgctcaagtt ctacgctaaa
     1921 ctgcgcgaaa cctccgcgca atcgtggctt tacgctccgg acggcggggg acacaccggc
     1981 aacggcacgc aacgcgaatc cgccgacgaa atggcgtgcg tcttattgtt cctcaaagaa
     2041 tttttggggt aagggcgggt agcggcactg ctgccgcgaa tgaaaaaagg tcgtctgaaa
     2101 ctgctttttc agacgacctt ttttaatggt tgtttcaaat caaaatatct atgccgccgg
     2161 ccccatcagc acttcttcgc atccgaaggc aaaaatccgt aatgccgcct gaacgcttcg
     2221 ttgaaccgtc ccgcgtggcg gtagccgcaa aagtgcatgg cggcttggac ggtgctgccg
     2281 gattcgatga gggcgagcgc gtgttccagc cgcaggcggc gcaggcatcc ggcgacggtt
     2341 tcgccggttt gcgctttgaa atagcgtttc aggtagcatt cgttcagtcc gacgcggcgg
     2401 gcgatttcgg cgatggtcag cggacgggcg aattcgtgtt gcaggatgtc ggcggcttcg
     2461 tctatgcgcc gacggcggta accgttgtcg tggcggcgga aggtgaagcg caataatcgg
     2521 gcggagagtt ccagcgcggc ggcttcgtcg gcaagcaggc cgaagccgtc cgattcgaac
     2581 gggcgttgca gcagtgggca ggccgccgcc gtcagtgccg ctgcgttttg cgccagccgt
     2641 tgcagggcga atcggcctat tgtttgcggc gaaaacaggc gttcgtccag caagccttcg
     2701 tcgtgccagc ggcgcagttt ttccagcgaa aaatccaaat gcagcgcgca catgccgctg
     2761 ttgtcgggca gcagggtttc ggatacgtcc gccaaatcgc cgcgtaccag tcagatttcg
     2821 ccggcagatg ggcggtattc cctgccgccc atttgtaacc ggttctgccc cgacaccatg
     2881 acgaacaagg cgcagttgtg gctgaaattg tggatttcgg tgggaaacgc gcccgttccg
     2941 ccgccgcgca tccgcgacaa ggtgatgccc gaatcgaagc ggttgatgca catttccaga
     3001 tgcaaaccgg gctgttttgc ctgcgcaatg agcgcgctgt cggaacagcc gtccaacgcc
     3061 cagccggatt tatcggagcg gacataggtt tggtactggc ggtagatggc ggcggtgttc
     3121 atgattggat aggaacgagt tgtctaacaa atgaattaaa taggaattat taccaataat
     3181 caagcgcagg gattggttga aacggaaaag gtcgtctgaa agggtgtttc agacgacctt
     3241 ttccgtatcg ggaatttgtt ttgccgtatc gggaattttg cgttttgcgg cgtggtttct
     3301 gcaggttgtt tgcttaataa taaacattct tattcgtatg caaaggaacc gcacaccgtg
     3361 aaaccgcgtt tttattgggc agcctgcgcc gtcctgctga ccgcctgttc gcccgaacct
     3421 gccgccgaaa aaactgtatc cgccgcatcc gcatctgccg ccacgctgac cgtgccgacc
     3481 gcgcggggcg atgccgttgt gccgaagaat cccgaacgcg tcgccgtgta cgactgggcg
     3541 gcgttggata cgctgaccga attgggcgtg aatgtgggcg caaccaccgc gccggtgcgc
     3601 gtggattatt tgcagcctgc atttgacaag gcggcaacgg tggggacgct gttcgagccc
     3661 gattacgaag ccctgcaccg ctacaatcct cagcttgtca ttaccggcgg gccgggcgcg
     3721 gaagcgtatg aacagttagc gaaaaacgcg accaccatag atctgacggt ggacaacggc
     3781 aatatccgca ccagcggcga aaagcagatg gagaccttgg cgcggatttt cggcaaggaa
     3841 gcgcgcgcgg cggaattgaa ggcgcagatt gacgcgctgt tcgcccaaac gcgcgaagcc
     3901 gccaaaggca aaggacgcgg gctggtgctg tcggttacgg gcaacaaggt gtccgccttc
     3961 ggcacgcagt cgcggttggc aagttggata cacggcgaca tcggcctacc gcctgtagac
     4021 gaatctttac gcaacgaggg gcacgggcag cctgtttcct tcgaatacat caaagagaaa
     4081 aaccccgatt ggattttcat catcgaccgt accgccgcca tcgggcagga agggccggcg
     4141 gctgtcgaag tattggataa cgcgctggta cgcggcacga acgcttggaa gcgcaagcaa
     4201 atcatcgtca tgcctgccgc gaactacatt gtcgcgggcg gctcgcggca gttgattcag
     4261 gcggcggagc agttgaaggc ggcgtttgaa aaggcagaac ccgttgcggc ggggaaagag
     4321 tagggagtcg tctgaaaacg gagcttccga aggaagcggg gggtttctgc gaagctaaag
     4381 tgcggtttca acgaattgaa aagcagcctg tattttgaaa ataccgctca agcaaaccta
     4441 cggtttgccg ccctctccct agccctctcc cacagggaga ggggattggg ttgcaggctg
     4501 cctttaaggt ttaggcaaat ttttaacttc gttgaagctc cgttttcaga cgaccttttg
     4561 gaacaccaca ggcacacgca tcgaacggct gaatcaaaga ttcagaccga tggcagtccg
     4621 cacccgagtt tatgcggcaa acagcgaggc tacggcaacc cgccccctct ccctgtggga
     4681 gagggttagg gagagggcag taagccgcag gcttacatca aagtcgataa cgctttcgtt
     4741 acaactccgc ccactgaacg aagccaaaac gacaaaccgc atcgtaaacc acccaaccca
     4801 taggagaacc ccatgcaaaa cgaaaccatc aacctgaaac agcaccttgc cgccatcaaa
     4861 gaatactggc agcccgaaat catcaaccgc cacgggttcc aattccgttt ggtcaaactt
     4921 ttgggcgatt acggctggca tacgcacgga tacagcgaca aagtgctgtt tgccgtggag
     4981 ggcgacatgg cggtggactt cgccgacggc ggcagcatga cgatacgcga gggcgagatg
     5041 gcggtcgtgc cgaagtcggt gtcgcaccgc ccgcgttcgg aaaacggctg ctcggtggtg
     5101 ctgattgagt tgtccgaccc gtccgaggcc gtctgaaaac gaagtttccg aaggaagctg
     5161 agtttctgcg aagctaaaag cagcctgcac cttcaatcaa tatgccgaaa atacaacccc
     5221 accgcacacc aacacacaaa ggaaatccca tgacacgctt caaatattcc ctgctgtttg
     5281 ccgccctgtt gcccgtgtac gcgcaggccg atgtttctgt ttcagacgac cccaaaccgc
     5341 aggaaagcac tgaattgccg accatcaccg ttaccgccga ccgcaccgcg agttccaacg
     5401 acggctacac tgtttccggc acgcacaccc cgctcgggct gcccatgacc ctgcgcgaaa
     5461 tcccgcagag cgtcagcgtc atcacatcgc aacaaatgcg cgaccaaaac atcaaagcgc
     5521 tcgaccgcgc cctgttgcag gcgaccggca ccagccgcca gatttacggc tccgaccgcg
     5581 cgggctacaa ctacctgttc gcgcgcggca gccgcatcgc caactaccaa atcaacggca
     5641 tccccgttgc cgacgcgctg gccgatacgg gcaatgccaa caccgccgcc tatgagcgcg
     5701 tagaagtcgt gcgcggcgtg gcggggctgc tggacggcac gggcgagcct tccgccaccg
     5761 tcaatctggt gcgcaaacgc ccgacccgca agccattgtt tgaagtccgc gccgaagcgg
     5821 gcaaccgcaa acatttcggg ctgggcgcgg acgtatcggg cagcctgaat gccgaaggca
     5881 cgctgcgcgg ccgcctggtt tccaccttcg gacgcggcga ctcgtggcgg cagcgcgaac
     5941 gcagccgcga tgccgaactc tacggcattt tggaatacga catcgcaccg caaacccgcg
     6001 tccacgcagg catggactac cagcaggcga aagaaaccgc cgacgcgccg ctcagctacg
     6061 ccgtgtacga cagccaaggt tatgccaccg ccttcggccc gaaagacaac cccgccacaa
     6121 attgggcgaa cagccgccac cgtgcgctca acctgttcgc cggcatcgaa caccgcttca
     6181 accaagactg gaaactcaaa gccgaatacg actacacccg cagccgcttc cgccagccct
     6241 acggcgtagc aggcgtgctt tccatcgacc acaacaccgc cgccaccgac ctgattcccg
     6301 gttattggca cgccgacccg cgcacccaca gcgccagcgt gtcattgatc ggcaaatacc
     6361 gcctgttcgg ccgcgaacac gatttaatcg cgggtatcaa cggttacaaa tacgccagca
     6421 acaaatacgg cgaacgcagc atcatcccca acgccattcc caacgcctac gaattttccc
     6481 gcacgggtgc ctacccgcag cctgcatcgt ttgcccaaac catcccgcaa tacggcacca
     6541 ggcggcaaat cggcggctat ctcgccaccc gtttccgcgc cgccgacaac ctttcgctga
     6601 ttttgggcgg acgatacacc cgttaccgca ccggcagcta cgacagccgc acacaaggca
     6661 tgacctatgt gtccgccaac cgtttcaccc cctacacagg catcgtgttc gacctgaccg
     6721 gcaacctgtc tctttacggc tcgtacagca gcctgttcgt cccgcaatcg caaaaagacg
     6781 aacacggcag ctacctgaaa cccgtaaccg gcaacaatct ggaagccggc atcaaaggcg
     6841 aatggcttga aggccgtctg aacgcatccg ccgccgtgta ccgcgcccgt aaaaacaacc
     6901 tcgccaccgc agcaggacgc gacccgagcg gcaacaccta ctaccgcgcc gccaaccaag
     6961 ccaaaaccca cggctgggaa atcgaagtcg gcggccgcat cacgcccgaa tggcagatac
     7021 aggcaggtta cagccaaagc aaaacccgcg accaagacgg cagccgcctg aaccccgaca
     7081 gcgtacccga acgcagcttc aaactcttca ctgcctacca ctttgccccc gaagccccca
     7141 gcggctggac catcggcgca ggcgtgcgct ggcagagcga aacccacacc gaccctgcca
     7201 cgctccgcat ccccaacccc gccgccaaag cccgcgccgc cgacaacagc cgccaaaaag
     7261 cctacgccgt cgccgacatc atggcgcgtt accgcttcaa tccgcgcgcc gaactgtcgc
     7321 tgaacgtgga caatctgttc aacaaacact accgcaccca gcccgaccgc cacagctacg
     7381 gcgcactgcg gacagtgaac gcggcgttta cctatcggtt taaataaggt cgtctgaaaa
     7441 cggagtttct gcgaag
//
LOCUS       DAWIVB010000104         7453 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWIVB010000104 DAWIVB010000000
VERSION     DAWIVB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.104"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(291..1103)
                     /locus_tag="WIX60_001438"
     CDS             complement(291..1103)
                     /locus_tag="WIX60_001438"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ5305696.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1305..1766
                     /gene="tsaE"
                     /locus_tag="WIX60_001439"
     CDS             1305..1766
                     /gene="tsaE"
                     /locus_tag="WIX60_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ5305697.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            1763..3013
                     /locus_tag="WIX60_001440"
     CDS             1763..3013
                     /locus_tag="WIX60_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ5305698.1"
                     /translation="MIKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKNRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADLAQNTTPQPGRGRNGRRPVIMLDPGHGGEDPGAISPGGLQEKHVVL
                     SIARETKNQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(3071..4213)
                     /locus_tag="WIX60_001441"
     CDS             complement(3071..4213)
                     /locus_tag="WIX60_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ5305699.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQTRPVRAPIAGSDNDRRIVQTALDNARRA
                     GVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WLAAMFTGDREMPKFMCLSPKRKIPLYNGNIDCRLFLIDMVEGSNR"
     gene            complement(4262..4684)
                     /locus_tag="WIX60_001442"
     CDS             complement(4262..4684)
                     /locus_tag="WIX60_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305700.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(4677..5486)
                     /locus_tag="WIX60_001443"
     CDS             complement(4677..5486)
                     /locus_tag="WIX60_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ5305701.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(5568..5945)
                     /locus_tag="WIX60_001444"
     CDS             complement(5568..5945)
                     /locus_tag="WIX60_001444"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ5305702.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(6145..6912)
                     /locus_tag="WIX60_001445"
     CDS             complement(6145..6912)
                     /locus_tag="WIX60_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ5305703.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(6922..>7453)
                     /locus_tag="WIX60_001446"
     CDS             complement(6922..>7453)
                     /locus_tag="WIX60_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ5305704.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLINVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1802 a         2043 c         1828 g         1780 t
ORIGIN      
        1 cccacaagct gaaatcccgt tattttcaca aaaacagaaa accaaaaaca gaaacctgaa
       61 attcgtcatt cccgcgcagg cggaaatcca gtgcgttgag cttcagctat ttagaataaa
      121 ttttgaaact ctaatcccgt cattcccacg aaagtgggaa tctagaacgt aaaatctaaa
      181 gaaaccgttt tatccgataa gtttccgcac cgacaggtct agattcccac tttcgtggga
      241 atgacgggat tttaggtttc tgtaatctcg ggcagctttc cgtcatcgtt ttaacccaaa
      301 gacaccattt caatctgctc catcgtcctg cccagaaaac gctcgccgat ggttctgaat
      361 ttcaaaggaa tatcgctgac gtaaaaacgg tagtcgggat tgttgttgtc ggtattgagc
      421 aatccttcct gagcaaggac gcgtgcggtt tcttcggccg ttgtaattgc agaatcaacc
      481 aacgcgacat tgcccgcctc cctgccgatt aagggcttga gcaagggaaa gtgcgtgcag
      541 cccaacacca gcgtatcgat gccgtctgca agcaatggtt tgaggtattc gcatacggtc
      601 aggcgggtaa cttcgtgttc cagccagccc tcttccacca aagggacgag cagcggcgcg
      661 gcctgcgtgc ggacgagcgt gtcggggttg ttcctatgga tggcgcgcgc ataagcattg
      721 ctgttgactg tcgtattggt ggcgataatg ccgattttat tgttgcgcgt cgttgccagc
      781 gcggctttcg cgccggcgga aatcacgtcc aaaacgggca tattgccggt tttttgacgg
      841 attttctgcc ccgccaccgc cgcaatcgta ttgcacgcga taaccatcgc cttgacatcg
      901 tgttccaata aaaaatcgac aatctgcatc gagaaatttt cgatggtcgc cttagatttc
      961 gtcccgtaag gcacgcgcgc cgtgtcgccg aaataaatga tgttctccat cggcagccgt
     1021 tccatcagcg ctcgcacatt ggtcaaaccg ccgattcccg agtcaaaaac gccgatgggt
     1081 cgctgcctgc cgatattttc cattcttttt ccaatccgtc ctaaacatac aatccgcgta
     1141 ttgtacacgc gccgcttttt cttgacagcc gttgccgtct gaaagcagaa cgcggatttg
     1201 gctgttataa tgctcggaca agaagcatcc gcccctcggg tgcaaagttt gcaaaacctg
     1261 caaaactgcc cataataccc tagaccttac aacaaaccgt ttccatgtct gatttaccct
     1321 ctatctcccg attccttgcc gacgaagccg ccacactcga tttgggcgcg gcgtggtctt
     1381 cccgtttaaa cgcaccgctg gtcatttatc tcgaagggga tttgggtgcg ggcaaaacca
     1441 cgctgacacg cggcatcctg cgcggattgg gtcatcaggg cgcagtcaaa agtccgacct
     1501 acgccatcgt cgaatcttat ccgctggaac gcttcaccct gcaccatttc gacctctacc
     1561 gcttctcgtt ccccgaagaa tgggaagacg cggggcttga cgaactgttt gccgcaaaca
     1621 gcgtctgcct gatcgaatgg ccgcaacagg gcggggaatt tacgccgccc gccgacatca
     1681 ccgcaacatt gacacacgac ggcgacggca gaaaatgcct gctgaccgcc cataccgaac
     1741 gaggacgcga aagcctgccg ctatgatcaa actgacacga agacaaatca tccgccgcac
     1801 cgccggcaca ctgttcgccc taagccccat cgcatccgcc gttgccaaaa cggtacgcgc
     1861 cccgcaattc accgccgcac ggatatggcc gtcgcacacc tacacccgcc tgacgctgga
     1921 aagcaccgcc gcgctcaaat accagcactt cacgctcgac aacccgggca ggctggtcgt
     1981 cgacatacaa aacgcgaaca tcaataccgt attgcacgga ctgtctcaga aagtcatggc
     2041 agacgacccc tttatccgca gcatacgcgc gggtcagaac acgccgacca ccgtccgcct
     2101 cgtcatcgat ttgaaacagc ccacccacgc acaagtcttc gcgcttccgc ccgtcggcgg
     2161 ctttaagaac cgcctcgtcg tcgacctcta tccgcacggg atggatgccg acgatccgat
     2221 gatggcactg ctcaacggta gcctgaataa aaccctgcgc ggctctcccg aagccgacct
     2281 cgcccaaaac accacgcccc aacccgggcg cggcagaaac gggcgcagac ccgtcatcat
     2341 gctcgatccg ggacacggcg gtgaagaccc cggcgccatc agcccgggcg gtctacagga
     2401 aaaacatgtc gtcctatcca ttgccaggga aaccaaaaat cagttggaag cattaggtta
     2461 caatgtattt atgacgcgca acgaagacgt gttcatccca ttgggcgtgc gtgtcgccaa
     2521 agggcgagca cggcgggcgg acgtatttgt ctccatccac gccgatgcct tcaccagccc
     2581 ctccgcgcgc ggcacggggg tttatatgtt aaacaccaaa ggcgcaacca gctctgccgc
     2641 caaattcttg gaacagacgc aaaacaatgc cgacgcggtc ggcggcgtac cgaccagcgg
     2701 caaccgcaat gtcgataccg ccctgctcga catgacccaa accgccacgc tgcgcgacag
     2761 ccgcaaactc ggcaaactgg tgcttgaaga attgggcagg ctcaaccatc tgcacaaagg
     2821 cagggtggac gaagccaatt tcgccgtttt gcgcgcaccc gatatgccgt ctatcctggt
     2881 cgaaaccgcc ttcctgtcca atcctgccga agagaagctg ctgggcagcg aatccttccg
     2941 ccggcagtgc gcccaatcca ttgcctcggg tgtccaacgc tacatcaata catccgtatt
     3001 gaagcggggt tgattccgaa aaataaatgc cgtctgaaca tttttcagac ggcatttttg
     3061 tacactttcc tcaacggttc gatccttcca ccatatcaat caggaacagg cggcagtcga
     3121 tgttgccgtt ataaagcggg attttccgct tgggcgacag gcacatgaat ttgggcattt
     3181 ccctatcgcc ggtaaacatt gccgccaacc agcctgcgta atgttttttc aaccacgtcc
     3241 ccaactgcgg atacagtgcc tgcaaggcgc ggacttcctc aaggcgcacg ccgtagggcg
     3301 gattggacac cataatgccg ttttcgccgt tcggtcggac ggactgcgcg tcggcaacgc
     3361 tgaaggaaac gatgtcgtcc accccggcgc ggcgtgcgtt gtccaatgcc gtctgaacga
     3421 tgcggcggtc gttgtcgctg cctgcaatcg gggcgcggac ggggcgggtt tgcgcttcgg
     3481 cgcggcgccg caaatccgac cacagcgttt tatcgaaatt ttgcagtttt tcaaaaccga
     3541 aacggcgcat catacccggc gcgcggcggg cggcaatcca agcggcttcg atagcaatcg
     3601 tgccgctgcc gcaaaacggg tcttgaaacg gctgcgtgcc gtcgtagcct gccgagagca
     3661 gcagtccggc ggcaaggttt tcgcgcagcg gggcttcgcc ggtatccagg cggtagccgc
     3721 gtttgaacag ggcttcgccc gaagtgtcaa taaagatttc gacattgcgt tcgttcaaaa
     3781 aggcgtggat gcggacatcg ggcgcggctt tgtccacgct cggacgtgcg tcgtaaatgt
     3841 cgcggaaagc gtcgcagacg gcatctttga cggtcagtcc gacaaattgg atgctcttaa
     3901 cgttggcacg ctttgcctcg actttgactt tgaacgtctg ctgtaaagta aaccaattaa
     3961 accagttgat atttttggcg agtttgtaga tgtcgcgctc attgcggtat gtccctttgg
     4021 tcaggcgcag caggatacgg ctggcagtac gcgaatgcag gttggcggcg taaacctgtt
     4081 ccaatccgcc ccggcaggaa acgccgccgt caaacacttg tacatcggta cagccgaggc
     4141 tttcgagttc ttgagataaa acggtctcca agccgcgcgg gcaggtggcg aaaagtgtat
     4201 ataaagtatt cataacgtgt cctttccgga cggatgttcc ggcaatggcg gattgaaagg
     4261 tttattcggc ggtaaatgcc gtctgaaatg tccgactgcc gattgtaatg tccgccgtaa
     4321 aatcgcgcct gccttcgaca cagatgggca ggcggatgcg tactgcctgc caagtccccg
     4381 acggttgccg ctcgaacata tagcggttga aacccatatc catatttttc atactgaagc
     4441 tgatgctgac ctgttccgtg ccggcgggcg cgtgttcgat ataaatatca aacggttttt
     4501 tggttgaaac ggcggcggcg cggacgcggc ttccgtccgg cagcgtgcaa ccctcggtca
     4561 aatcgcattg cgccgccaca gcttgcggct gctgcgcctg ccaccattgc aacaaaacga
     4621 gcttgacggc ggcaaaggca atcagcagca gtgcggcaag cagtaattta cggtttttat
     4681 tcatgattct tccctattgt ttcatccctt accaccacgt gcgcccccaa tccgagccat
     4741 agttttccat aacgtgcaac cgtctgtccg tttgctgcaa gtacgagcgg cacggatacg
     4801 ccctgccgca gggcttctgc cgcctcttgt gcctgaacat catcgcacac gcgccaaacg
     4861 accgcatccg cgcccttttc ctgcgcccgc cgccattgtt cgcgggtctg caccaccatc
     4921 cacacgctgt cggcgtgttc gggtctgtcc tgccattgcg ccgcctccat caaaacgttc
     4981 gcaccgcttc cctctgccga acccaaaggc aggacgcggt acgcgcccat actgttttct
     5041 ccgtgcaaac ccgttttcag gctgccgtac aaacggcgcg ggacggacag cgaacgcaaa
     5101 agcgcgccgt tggcgggcag catgggggca acggtaaaat cacccgcctt ctgccaagac
     5161 cattcctgcc cttcgcggga ttgcggtttg cccgtccatt ggtcggggtt gacccatagg
     5221 aatttcaggc agacgcgggc gtgttcgtag gaatggattt tggtcaacca aggcgtggcg
     5281 gcgaggatgc ggatgccgag ttcttcttca aactcgcgtt gcagggcttg gaagtcggtt
     5341 tcgcccgctt cgaccttgcc gccggcaaat tcccaatatc cggcataggg tttgccttcg
     5401 gggcgcgagc tgagcaggta gttgccgtct gaatcgagca ggatgccggc aacgacgcgg
     5461 ataaggggtc gggtgtcttg aatcatggcg ggtacgcggt tggtcggtcg gagtgcaaat
     5521 gagggacggg caatgccgtc aacgggtttg ccgcatttca gacggcatta tttttcggca
     5581 acgacaaacg ccaatacggt gtcttcttcg tcgctcatgc tgaggctgac gcggctgatg
     5641 ccttgttcct ccagccattt ggacagggcg gggccgtaga aaaattcggg cttgcccaat
     5701 gcgtcatgcc cgatgccgat gttgcggaag gaaaccgcgc cgcgtatgcc cgtgccgacg
     5761 gctttggcaa aggcttcttt ggcggcaaag cgtttggcga ggtagttgac gggtttgccc
     5821 gcttgcggaa attcaagcag ctcttccgga gtgaggatgc gcccggcaaa cgcctgtccg
     5881 aattttttgt ttaagcggat gatgcgcttg agggaaacaa tgtctgtgcc gatgccgtaa
     5941 atcatatttg cgctccttcg cccttggttg cgggtagtga tgaatgtgat gatgaatgcc
     6001 gtcaatttct tggggcgtta aaatggcgtg aatatccact tttggggttt tgtcggtaac
     6061 aattttcact gtaacctttt gcatttgaaa ttctaaaaca tcgtgccaat tatttaataa
     6121 ggagttaagg catttaataa tgaattaaag tttgattaaa cccgaatgcc cgattttcag
     6181 agccaatacc aataaaacaa taaaaattaa cattttaaaa aaatttgggc taatcttagt
     6241 ccttaaccga attccaacat acaatccaat aacagacaat acggacagta aaaatattaa
     6301 accgtattca ctcttattta ataaccaata ctggtctctt agcatatata tttgaacaat
     6361 tttcgccaaa agatagcata gattgcttga ttttacgata cgatttttat tttctgtttc
     6421 gctaagcaaa aatattaaca atatgggaga catggcattg gttgaaccgc cgatgatgcc
     6481 tgccaaaaac ccaaaaagaa ccatattctt attattggca actacttgaa tattttttgc
     6541 ttttgcacat acatttaaaa taccattgac agaataatac aatgtaatga ttgccatcag
     6601 taaaagcagc caagacactg gaagtatcaa aagcaacttc acccccaaaa tgctgccaac
     6661 gacgctgccg atagcaagca atttataggt ttttaaataa taaacaatct cttgccaaaa
     6721 accctttttg ttattgctgc atagaaccaa caagctcatt aacaggcttg gtaatgccac
     6781 caaggcaaca accttagaca atggcatgat aaaagccaat gcggttgtac cgagcatcgg
     6841 aaatcccatg cctgtaattc cgtgcagtat tgcggcagca acaaaaacga tagattgcat
     6901 tatttcttgc ataaccgtcc tttatacagt aattcttgct ctaatataat gcctgttttt
     6961 atccgtataa tcagttctgg catgcaagcc attgacattg tcaataataa tcaaaccatc
     7021 atcttgtgca gaaaattctt gaatatcact cgcatttttt ataacattta taagataatc
     7081 aagtgcaagt accatttcct ttgatacttt atttctattt tcttcaatac ctttatagat
     7141 acaatcactt ctaaatctaa tcatttgagt attgaccgat aagatattac cccatctcac
     7201 accttgtttt ttatcaaatg atgctggtgt tttaaatgga tataaattgc ccgttaatgt
     7261 ttttaagtgt tttttaccgg tttctgtttt agataactga ttgacaatat ctgatgaact
     7321 taaaaatagg gaattacctc catcattggc tgattttact acatacataa ccaaataact
     7381 ttccgggttt tcactaaaag atgaatcact atgaagtgga cattctccaa catcttccga
     7441 gaaagttata tca
//
LOCUS       DAWIVB010000105         7445 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWIVB010000105 DAWIVB010000000
VERSION     DAWIVB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.105"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(205..2808)
                     /gene="pepN"
                     /locus_tag="WIX60_001447"
     CDS             complement(205..2808)
                     /gene="pepN"
                     /locus_tag="WIX60_001447"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ5305705.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYMLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYAEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(3019..3813)
                     /locus_tag="WIX60_001448"
     CDS             complement(3019..3813)
                     /locus_tag="WIX60_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ5305706.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4563..5432)
                     /locus_tag="WIX60_001449"
     CDS             complement(4563..5432)
                     /locus_tag="WIX60_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ5305707.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPGEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5483..6019)
                     /gene="ruvC"
                     /locus_tag="WIX60_001450"
     CDS             complement(5483..6019)
                     /gene="ruvC"
                     /locus_tag="WIX60_001450"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ5305708.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAS
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(6022..6261)
                     /locus_tag="WIX60_001451"
     CDS             complement(6022..6261)
                     /locus_tag="WIX60_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ5305709.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6292..7302)
                     /gene="dusB"
                     /locus_tag="WIX60_001452"
     CDS             complement(6292..7302)
                     /gene="dusB"
                     /locus_tag="WIX60_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ5305710.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRQN
                     AV"
BASE COUNT         1670 a         1976 c         1947 g         1852 t
ORIGIN      
        1 acaaaaacca gtacagcgtt gcctcgcctt accgtactat ctgtactgtc tgcggctttg
       61 tcgccttgtc ctgatttttg ttaatccact ataattaata tgactttgcg gccgttttgc
      121 cattgcgtaa taaaacgatg gggaagtgat gataaaacgt gtgtgtaact atatcagacg
      181 gcattgtttt tctgtttgac ggcctcaatc caaaattttg ccgacgattt cgcccacgtc
      241 tttcgacaat ccttcctgcg cccgaatgcg ctgcaatgct tgtttcacca agtttttgcg
      301 gtgcggttcg agcttattgc agagattgaa cgcctgcact aggcgggcgg cgacctgcgg
      361 gttaaagcgg tcgatttcga tgactttgtc ggcgatgaag cggtagccgc tgccgtcttc
      421 tgcgtgaaaa tgcggtacgt tgcggctgaa gctgccgatg agcgagcggg ctttgttggg
      481 gttttcgagg ctgaacttcg gatgctgcaa ggcggtttga acctgttgca gggtgtcgct
      541 gcggcggctt gatccaacga gggcgaaata tttgtccatc accagcgcgt cgtctgaaaa
      601 cttgtcggca aactgcgcca gcaggcggtt gcgcgtatcg ctttcgttgc cgttgacggc
      661 ggacaggatg ccccattcgt gggtcatgtt ttgcgccatt tcggcgtatt tctcggcaac
      721 ggtttcgatg tgcgcgggat cggcgcgcag gacgaaggcg cggcagacat tgcgcagcgt
      781 gcgccaaccg gcggcttcgg ggctgtattc gtagctttgg ttttcctgct tcgccgcctg
      841 acggttcaat tcgtgccatt tcggcaggaa gcggacggca agcgtatcca acaaggcttc
      901 gcgcgcctga tggtagcgca gcgggtcgat gttttccgcg ccgtcccaca gctcggcttc
      961 agacggcacg cccaaaagca gggctttgaa ggcgttgtcc aagaggtcgt ctgaaatgac
     1021 tttttcgacg gcggcaagca gtttttcgtg tttcggcaac ccgatgccgt ctgaaagcgc
     1081 ggcaaggttg gcggcgacgg cgcggcggta gagcgtttgg gcggcttccc agcgcgtgaa
     1141 ggcgtcgctg tcgtgggcga gcaggagcag caggtcgtcg tcgctgtacg gatagttcag
     1201 atgcaccggc gcgctgaacc cgcgcagcag cgagggaacg acggcttcgg ttacgccttc
     1261 gagcaggaag atctgttcgg cttcggtcag cagcaatacg gcttcggttg cgcgtttgcc
     1321 ctgataatcg aatgccgccg cttcgccgtt gcggttcagc agcccgattt tgacgggaat
     1381 catcatcggc tgcttgtccg ccatatcggg cgtgggcggc acggtttgtt tgatggttaa
     1441 ctcgaaacta ttgtttttca gacgaccttc cgcttccaag acgggcgtac ccgcctggct
     1501 gtaccacaag gcgaactggt cgagattgat gccgttcgcg tccgccatcg ccgcgcggaa
     1561 atcgtcgcag gtcacggcct gcccgtcgtg tcgttggaaa tagagcttca tgcctttttg
     1621 gaagccctct tcgccgagca aggtgtgata catccgcacc acttccgcgc ctttttcata
     1681 aacggtcatg gtgtagaaat tgttcatctc ctcatagctg gcggggcgca ccggatgggc
     1741 ggtcgggcct gcgtcttcgg ggaactggtg ctggcgcagc aggcggatgt tctcgatgcg
     1801 gcgcacggcg cggctggcgc ggtcgccgga aaattcttgg tcgcggaaca cggtcaaccc
     1861 ttctttcagc gaaagctgga accaatcgcg gcaggtaacg cggttgcctg tccagttgtg
     1921 gaaatactcg tgtccgacca cggattcgat gccttcaaaa tcggtatcgg tggcggtacg
     1981 gctgtcggca aggacgaact tggtattaaa aatgttcagc cccttgtttt ccatcgcacc
     2041 catattgaaa tcgcccacgg cgacgaccat gaaaatatcc aagtcgtatt ccaaaccgaa
     2101 gcgcgtttcg tcccacttca tcgcgttttt caaggattcc acggcaaagc cgaccttagg
     2161 cttgtccgcc tcggcagtgt aaaactcgat tttgacgttt ctgccgccca tcgtggtgaa
     2221 acagtcttcc gtgaccgcca aatcgcccgc gaccaaagca aacagataac tcggtttggc
     2281 aaacgggtct tcccatttca cccaatggcg gccgtctgaa tactcgccgc cgtcgatttt
     2341 gttgccgttg gagagcaaaa cgggatagcg ttttttgtcc gcgacgatgg tggtcgtgaa
     2401 cttggacatg acatccggac ggtcgatata gaacgtgatt ttgcggaagc cctccggctc
     2461 gcactgggta aacaggttac cggcggacgc atacagcccc atcagcgatt tgttttccgc
     2521 cggcaggatt tcggtttcca cttcgacggt gaagcgttcg gacggcacgt ctgcaatcgt
     2581 cagcgtctcg ccttccaaca tataatccgc cgccgcgccg ttgattttga cggacaagag
     2641 tttcgccgaa ccgtccaaca ccagcggctc ccccaccctc tgcggctcaa ccgtcaaacg
     2701 cgacttcacg acggtttgcg gttcggcaat atcgaaatgc aggtcggttt cgagaatgcg
     2761 gtaggcaggc gtttggtaat ctttcagata acggacggtt ttgctcattt ttttctttca
     2821 atattgtttg gtttgactgg aaaaggcttc agacggcacg gatacagctt ttaaagcgga
     2881 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt actgatagta cgggctgtac
     2941 cggtttgaat ttaatccacc atatgccgtc tgaaccgaaa cggcggcaca agcgtaccgc
     3001 cttcggacag cagattttct atttccacgc ttggacaaat tctgcccaat gcggtttgtc
     3061 ttctgtttgg gcggaaagat agtttctgat ccgcttgatt tccatttcgt attcgtccgc
     3121 gtcgaatctg ccgctgacca gacaaaaacg cagatacatc aaatacgtat tcacggcatc
     3181 ggtttcgcaa taattgcgga tttctttcag cctgcccgcg tggaacgcat cccacacctt
     3241 gctgccgtcc atacccagct tgcccggaaa accgcacagt ttcgccatat cgtccagcgg
     3301 cacgtttgcc ctcggctgat aaagtgcgag caaatccatc aaatcgcagt ggcgttggtg
     3361 gtaacggctg atgtagttgt tccacttgaa atcgcggctg tcgccgaaat cgccgtcgcc
     3421 catatcccaa tagcgcgcgg cgttgatgcc gtgtagcagc gaacggtaat gcagaaccgg
     3481 caggtcgaaa ccgccgccgt tccaactgac cagttgcggc gtatgttttt caatcaattc
     3541 gaaaaattta gcaatgacca cttcctcgcc gtcatccatc tcgccgatgg tgccgacatg
     3601 tactttatcc tgcccccaac gcatgcagca cgaaatcgcc acaacctgat gaagatgatg
     3661 ctgcataaaa tcgccgcccg tctgagcacg gcgtttttgc tgggcaaaca gcaccacttc
     3721 atcgtcgggc agcgaggacg gcagctcgta caatgttcgg ataccctgga catcaggtac
     3781 ggtttcaata tcgaaagcca aaatcgtggt catgacagca ccttgtattt aaaacagact
     3841 tgcgcctatt gtgtcattaa aaggcggata aaaaaagagg caacccccca caggattgcc
     3901 ccaatacctc aaatcagaga tttacgcttc acaaacaata caggctttcg cctgcggctt
     3961 tgcccgcgta gctcaactct acgccggcaa acttttgttt caccgtttcc gatgaaaccc
     4021 cgaccaatcg caagactgac cggaaaatcc tttcagacgg catttcctgc ctgtcgtgta
     4081 attccatgta gcgaaatgta cgccattttc tacgctttgc caagcatttt ttacaatata
     4141 aatgtcaaaa cattaatttt ataaaattgc tgaaaatatt aaatatatgg atttttattt
     4201 tttatatttc aataaatata aatttaattt tgatttatat ttaaatttaa gcataaaatg
     4261 tcaaatatta aaggaaatat gaaaagcata tattaaatat ttatttataa cactatgttt
     4321 ttaaagaaaa ttaattttaa tatattaact agattgtctg catatattca tgggtttgcg
     4381 gtatttcttc caaaacctgc ttcaaatttc ccgaccaagt cttaaaaata ttgttttttg
     4441 agatacttaa atagcagcga ttatcaaatg aaatctgttc atataatctg ccatttcgca
     4501 tttaaaaaaa caaacaggag tttcgactcg aaacgcctga tatgttttgt aattttacgt
     4561 agtcagtaaa aatcggggct gccttccgga cgggttttaa aacgcttgtg cagccaaaaa
     4621 tattgttccg gatgttcgcg cacccgctct tcgataaagc ggttcatacg ctgcgcatcg
     4681 gcttgcgcgt cttcacccgg aaaggatttc caagccgggt agaattgcaa tgtaaccgta
     4741 ttgtccgcct cgcggacagg gatggcgggt atcacttttg catttgcaag cgcggcaatg
     4801 cggctcaatc cggtaatcgt tgccgtctga ataccgaaga aatccacaaa aaccgaatcg
     4861 ttgcgtccga aatcctgatc gggcagatac agaaacggcg cgctgctttt gcggaactgt
     4921 ttgacgaggg cgcgcagccc ttcggtgcgc ccgataagga agacgttgtc gtagcggttg
     4981 cggcctttca aaatctgtgc gtccaatatc ttgttttttt ggtgggaata catactaatt
     5041 agcgggacat cctgattaag cgcgtacacc gccatctcga acgcggtaaa gtgcggatac
     5101 aggatgatga ctttttcccc cgccgccagc gcgtcgtcca aataatgctt attgcggtag
     5161 cgcaccagcg atttcaaacg cccggcgggc gcgtaccaat ataagccgta ttcgagcatc
     5221 agtttcgcca tatgtttgaa atgctgtttc aacacggttt tacgcttttc ctcactccat
     5281 tcgggaaaac atttcgccaa attgatttcg ccgatacggc ggcggggttt gaccaaaagg
     5341 taggcaagca aacccgtcag atcggcaagt ttgtgcagca gcgcaaacgg cagaaactgc
     5401 aaaacataca gtacaaaaaa tataaatttc atctcgatac acattttctt ttcagacggc
     5461 aaaatacaaa tgccgtctga aactattgaa acctgccgcg cttgacctgc atccccgaag
     5521 gattgagttt ggcggcaagc ccgtggttgc gtaaggcgtg ggtcagcgcg acggcaagac
     5581 cgtccgccgc atccgcctgc ggcgttcccg aaagccccag catctgcacc accatatgct
     5641 gcacctgttc tttcgccgcc ttgcctttgc cgactaccgc ctgtttgacc tgcaaggccg
     5701 tgtattccga aacgggcagc ttatggctga ccaatgccgc caatgccgcg cccctagcct
     5761 gacccagcat cagcgtcgat gcaggattga cgttgacgaa cacctgttcc actgccgcct
     5821 gttgaggctt gtaaacggca acgacttcgc cgatatgccg cacaatcacg gcaatcctgt
     5881 ctgccagaga cgcatcggca ggcgttttga tgcagccgga ggcaacataa aaatgatccc
     5941 gccccctgac atcgatgatg ccgaaacccg ttacgcggct gcccgggtca atgcccaaga
     6001 tacggacggt tgccgacata ttcacaacaa accgtgttga atcagttttt tacgcaaggt
     6061 attgcggttc agccccagca tcacggaggc tttggactgg ttgccgccgc attgctccat
     6121 cacgcacacc agcagcggtt tttccacctg atgcaagacc atatcgtaca cgccgcaagg
     6181 ttcggtaccg ttcaggtctt tgaaatattg ttccaaattt tgtctgatac attgggaaat
     6241 atcgggaagg gtatggggca taatcgcgct ttcaaaagga taatcgggtg ttcagacggc
     6301 attttggcgg taggcgcacg cccaacggtc ggtttttccg gcaagtcttt caagataacc
     6361 tgcaagcatg tcgtattgcg ccgccgcact gtccaagcgg ttgatttcac gacgtgtctg
     6421 ttcgccgtcg ggcatttcgt cgatgtacca gcctatgtgt ttgcgtgcga tgcgcacacc
     6481 ggcggtctca ccataaaacg cgtgcatggc gcggacgtgg ttcaaaatgg cggcactgca
     6541 ttctgccaaa ctcaaggcag gcggtaaaac accgtgttcg gcgtaatgtt tcaaatcgcg
     6601 gaagaaccac ggtctgcctt gcgcgccgcg ccctatcatg atgccgtctg cggcggtttg
     6661 tttaaggacg gcttgggctt tttgcggcga agtaatgtcg ccgttgaccc aaaccgggat
     6721 gttcagacgg catttggttt cggcgatgag ttcgtaacgc gcttcgcctt tgtacatttg
     6781 cgtacgcgtg cgtccgtgga cggcaagggc ggcgatgccg caatcttcgg cgattttggc
     6841 gatgacgggc aggttttgat ggtcgtcgtg ccaacccaaa cgggttttga gggtaacggg
     6901 tacgcctgcc gcacggacga cggcttccaa aatggcggca accagcggct cgttctgcat
     6961 cagcgcgcta ccggcttgga cattgcagac ttttttagcg ggacagccca tgttgatgtc
     7021 gataagctgc gccccaaggc tgacgttgta acgcgcggca tccgccatct gctgcggatc
     7081 gcttccggca atctgcacgg caacaatgcc gccttcatcg gcaaaatcgc tgcggtgcaa
     7141 ggtttttcta gtatttctga gcgtcgggtc gctggtcagc atttcgcaca ccgcccaacc
     7201 tgcgccaaaa tctcggcaaa gtcggcggaa cggtttgtcg gtaatgcccg ccatcggcgc
     7261 aagtgcgatg gggttgtcga taaaatagcc gccgatgtgc ataatggatc cgcgtttcaa
     7321 aaaagtacgc cattgtacat tttttaagca ggatttccaa tctccggacg cgcccgcgat
     7381 tgggtcggac accgttttat ggcataatcc gcacacagat tccctgcccc gccactcaca
     7441 ggcgg
//
LOCUS       DAWIVB010000106         7443 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWIVB010000106 DAWIVB010000000
VERSION     DAWIVB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.106"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(283..1038)
                     /locus_tag="WIX60_001453"
     CDS             complement(283..1038)
                     /locus_tag="WIX60_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ5305711.1"
                     /translation="MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(1163..2335)
                     /gene="nirK"
                     /locus_tag="WIX60_001454"
     CDS             complement(1163..2335)
                     /gene="nirK"
                     /locus_tag="WIX60_001454"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ5305712.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAETPAAAAEAASSAAQ
                     TAAETPSGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMTMEDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASASEKSVY"
     gene            2706..4961
                     /locus_tag="WIX60_001455"
     CDS             2706..4961
                     /locus_tag="WIX60_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ5305713.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELVAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     SDDPKFQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            5140..5673
                     /locus_tag="WIX60_001456"
     CDS             5140..5673
                     /locus_tag="WIX60_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ5305714.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5736..6920)
                     /locus_tag="WIX60_001457"
     CDS             complement(5736..6920)
                     /locus_tag="WIX60_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ5305715.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDTQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6960..7364)
                     /locus_tag="WIX60_001458"
     CDS             complement(6960..7364)
                     /locus_tag="WIX60_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ5305716.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPNLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1676 a         1798 c         2101 g         1868 t
ORIGIN      
        1 ggattcccgc tttcgtggga atgacgggat gtaggttcgt aggaatgacg aaaagttgcg
       61 ggaatgacgg aaagtggcgg gaatgacgga aagtggcggg aatgacggaa agtggcggga
      121 atgacggaaa gtggcgggaa tgacggttcg ggcattcctt aaattacccg tgtatcgctg
      181 taaatcttag agatggcgga atatagtgga ttaacaaaaa ccagtacggc aaggcgaggc
      241 aacgccgtac tggtttttgt taatccacta taattgaagg ggttatcggc ttgtgcaacg
      301 gaagcccaag ttgtgcaaga catatttgga ttgcaggctg gtacggatgc cgtagcggag
      361 gaaggcggca tagttggacg agtcgctcga cccgatagac gcgccgctgc aaaacatttg
      421 cgcgttggca ttgccggaag aaagcaggct gctgttgaaa tcttccgtcc attcccaaat
      481 cagcccgtgc atatcataaa cgccccagta gttcgggcgg cctttgccga catcgtgcag
      541 gcctttccgt ccgccgtcgg cataccaatc gagaatagtg cggttgtagc cgggttcgtt
      601 tgagccgttt ttctgcgtgg cggaagcaag tccggcaaat tcccattcgt caatggtcgg
      661 caggcgtttg ccttgtgcgg cgcaataggc gttggcggca aaccaggaaa cattggttac
      721 cggttgtttt aattcgcccg ccttcggcgc atagctgcgg ctgccgtttt tcatccaatg
      781 cttcaggtaa gcgggttctg cctgtttgga accgatcctg cctttttgcc attgggggtg
      841 gctgttgaca aattcggcaa actcggcatt ggtaacggga tatttatcca gtttgaacgg
      901 tttgacttta atcaggccgg tatctttttt cagataaagc gggcggtagc tgccgccttc
      961 gatttgaacc atttcggcag ccgccgcttg agtgccggcg agtgccgcgc cgaggaaaaa
     1021 taaccggaca tacttcataa agcctcctga caggcggtta aaatcaatct tccgaaagga
     1081 aagattggtt gttaaaaaac caccgccgtg cgtaatgaag tacagcggca gtggttcgtc
     1141 ccgctaatga cgggtatcca atttaataaa cgcttttttc ggatgcagag gctgccgggg
     1201 cagaagctgc gggagcggaa gcagcaggag ctgcaccgtt accggcgtaa gcggtatcac
     1261 tcaatttttg agtcatgatt tcagggtttt ctgcaccttc tactttcaat tgacccagtg
     1321 cgcctttgtt gaatgcgcgg aagatagagt ggtcaaccaa agtgtagctg cccgggatgt
     1381 cgactttgaa ttcgacgatg gcagagccgc cggcaggaac gatggtgctt tgtacgtttt
     1441 cgttaatcag tttgccgcct tcaacataaa ctttgtcgaa gatttcgccg atgacgtgga
     1501 aggaagatac caagttcgga ccgccgttac caacgtacat acgtacagtt tcgcctgctt
     1561 tggctttcag cgcgttatcg ccggcgatag cacctacgtg accgttgaat acgacgtatt
     1621 caggctgttc ggcaacggct ttgtccatat cgaacggttg cagaccttgc gcgccttttt
     1681 tgcctttggt gtagaagtcg ccttggacga tgtagaactc tttatccact ttcggcaggc
     1741 cttctttagg ctcgaccaaa atcagaccgt acataccgtt ggcgatgtgc ataccgaccg
     1801 gtgcgacggc gcagtggtag atgtacagac ccggttgcag ggctttgaag ctgaatgtgg
     1861 aagtacggcc cggagcggta aaggttgcgg ccgcgccgcc gccctggccg gtagccgcgt
     1921 ggaagtcgac gttgtgcgga acggtagaag aaggattgtt ggaaaattcc acttcaaccg
     1981 tatcgccttc gcgtacgcgg atcatacggc ccggaacgtc gccgtcaaat gtccagtagc
     2041 ggtattccac accgtcttcc atggtcatgg ttttttcgac ggtttccatt tttacgcgga
     2101 ctttggcggg gtagtcgcgg tcgattgcag gaggcacttc gggagcgtgg gtggtaaccg
     2161 catcgataac gggcagttcg ccggacggtg tttcggcggc ggtttgtgcg gcggagcttg
     2221 cggcttcggc ggcagcggca ggggtttcgg caggggcttg cgcggcaggt tcgccgccgc
     2281 aggcggctaa tgcgaataag gaagcaatca ttgcagctaa ggcttggcgt ttcataatat
     2341 tttccttttg taagaaaagt agagggattg gttaagaact tggtgtgatt atgcgtttgc
     2401 cccattgggg gcattttgat ttaagtcaaa taattcataa aatatgaact taaaatgata
     2461 cttctaatac tttatagcta ctaaaaatta caataaatag gctgatttgc ttaagggctt
     2521 cggcaaatca ggcaaaggca gggtagttgc cgactaaaga ataatgatag ttattatcat
     2581 atatgtattt gttttatatg tgagtttctt tgaacaaatg tttatttgcg cggtaaaccg
     2641 tgctacaatc tttaacattc atattttgtg aattttaatc cactatatta aaaggagctc
     2701 tcaaaatggg acagtacaag aagctttggt acttgctgtt tgccgttctg gcggtatgct
     2761 ttaccattct tggctatatg ggcagcgagg tttacaaaaa agccccgcct taccccgaac
     2821 aggtcgtttc cgcatccggc aaggtcttga tgacgaaaga cgatattttg gcaggtcagt
     2881 ctgcatggca gactaccggc ggtatggaag tcggttcgat tttgggtcac ggtgcatatc
     2941 aggctccgga ctggacggcg gactggctgc atcgtgagtt ggttgcctgg ttggatttga
     3001 ccgcgcaaca gacttacggc aaaaaattcg acgaggtttc ccccgaggaa caagccgtct
     3061 tgaaaacacg cctggccgac gaataccgca atcaaagccg cgtgaaggaa gacggcagcg
     3121 tcgtcatcag cgatacgcgc gtgaaagcca tcgaaagcat cctgccttac taccacggtg
     3181 tgtatagcga cgatcctaag ttccaaacca ctcgcgaaca ctttgcaatg aaaaacaata
     3241 cattgccaag ccaagaagcg cgtgaaaaac tgttcaactt cttcttctgg acttcttggt
     3301 ctgcttcgac caaccgtcct gacgagaatt ttacttacac caacaactgg ccgcacgaac
     3361 ctttgatcaa caacgtaccg accactgaaa actatatgtg gtcattcacc agcgttgttc
     3421 ttctgctgat gggcatcggt ttgctgatgt ggggttattc cttcctgacc aaacacgagg
     3481 aagtggaagt gccgtctgaa gacccgattt ccaaaatcca actgacccct tcgcaaaaag
     3541 cattgggcaa atacgtcttc ctgacggtcg ccctgtttgt ggtacaagta ttgctgggcg
     3601 gactgaccgc gcactacacc gtcgaaggcc agggtttcta cggcattgac gaggcgttgg
     3661 gctttgaaat gtccgactgg ttcccctacg ccctgacccg tacttggcac atccaatccg
     3721 ccatcttctg gattgcaacc ggctttttga cggcaggttt gttcctcgcg ccgattgtca
     3781 acggcggtaa agatcccaag ttccaacgtg ccggcgtaaa cttcctctat atcgccctct
     3841 tcatcgtggt cggcggttct tacgcgggca acttcttcgc gttgacgcac atccttcccc
     3901 cggaatttaa cttctggttc ggacaccaag gttacgaata cctcgatttg ggacgtttct
     3961 ggcaactcct tttgatggtc ggtctgctgt tatggctgtt cctgatgttg cgttgcacgg
     4021 tttccgcctt taaagaaaaa ggcgtggaca aaaacctgtt ggctatcttc gtagcttcta
     4081 tggtcggtgt gggcgtattc tacgcaccag gcctgttcta tggcgaaaaa tccccgattg
     4141 ccgttatgga atactggcgt tggtgggtgg ttcacctgtg ggtagaaggc ttcttcgaag
     4201 tatttgccac tgccgccttt gcctttgtct tctacaatat gggctttgtc cgccgcagta
     4261 ccgccactgc ctccactttg gccgctgccg ccatctttat gttgggcggc gttccgggca
     4321 cgctgcacca cctgtacttc tccggctcta cctccgcctc tatggcaatc ggtgcctgct
     4381 tctccgcttt ggaagtcgtg ccgctggtgt tgctgggccg tgaagcatac gaacactggt
     4441 cttaccaaca cctgtccgac tgggcgaaac gcctgcgttg gccgctgatg tgcttcgtgg
     4501 cagtcgcctt ttggaatatg atcggtgccg gcgtattcgg cttcctgatc aatccgccga
     4561 tttccctgtt ctatatccaa ggtttgaaca cttctgccgt tcacgcgcac gccgccttgt
     4621 ttggtgtgta cggtttcttg gcattgggct tcgtattgct ggttgcccgt tatttgaaac
     4681 cgaacgcgca gtttgacgac aaactgatga cttggggctt ctggctgctc aacggcggct
     4741 tggtcggtat gattgccatc agcctgctgc ctgtcggcgc gattcaggca tacgcctcca
     4801 tcacgcacgg tctgtggtat gcccgcagcg aagagttcct gcaaatggaa atcctcgata
     4861 ccttgcgttg ggtacgtacg gcagctgact tggtctttat cggcggtgca atctgtgtcg
     4921 ccatccaagc cactaaaatc gtattcggcc gcgacaaata agttttgcgg tatgaaatga
     4981 gcaactgcac ccgttttcgg gtgcagtttt ttattgcgga ttgggacggg aatggaaaag
     5041 gatgtggatt ttttatttta atcggctata caattgctgc tttcatgccg tctgaagcac
     5101 gtttcagatg gcatcggttt acatcagata aggataaaaa tgggtattta cgattttcaa
     5161 atgaaagatg cagaaggcaa tgcggttgat ttgtcaggct atcgcggcaa ggttctgctg
     5221 attgtcaaca cggcaacgcg ttgcggtctg accccgcaat acgaggcttt gcagaagctg
     5281 tatgcacaat ataccgcaga aggcttggag attttggatt ttccgtgcaa ccagttccgc
     5341 gaacaggctc ccgaaagcag cggagaaatt gcccaagtgt gtatgatgaa gtttggcacg
     5401 aagtttaaga ttttcgacaa aatcgaagtc aatggagcaa ataccgcgcc cttgtatgcc
     5461 tacctgaaat ccgtcaaacc gcaagataaa ggcaatcatc tgtttaaaga tttcgtgttg
     5521 aagctcgcag ctttgggaga gaagcgcgac gaaggcgaca tcaaatggaa ctttaccaaa
     5581 ttcctcgtca accgcgacgg cgaagtggtc gagcgttttg cgcctagtgt tacgcccgaa
     5641 gaaatcgaag ccgatattcg ggcattgctg taatctgccg ccgtcttggg aaatgccgtc
     5701 tgaaggcttc agacggcatt tgtccgtttg cgggtttatt ccgaaatatc ggcaacaccg
     5761 ctttcgggga agatgggcaa atccggcggt gggagcaggg tatccaaagt cagggcggac
     5821 gcatcgagtg tggggtagcc ttcaaacgcg accgaataac cgctgtttcc gttcatacgg
     5881 attcgggcgt gcgcgcctag agggaaagtg attttgtcgg caatgtgtcc gaacgggaag
     5941 cccgtcagca cggggatttt cgccgtgcgc gaaacatggt tggcaacggc agaaaaatca
     6001 taagacggat catagacatc tcgaattttt tccatacgga aattgccgaa cacgatggcg
     6061 cgctgtttct tcaaaatacc cgaaagatac agcgtattga gcatacgttc gatgcggtag
     6121 ggctgttcgc cgacatcttc gaggaacaaa atgccgccgt cgatgtcggg catataaggc
     6181 gtgccggcga gcgaggcgag gacgcttaag ttgccgcccc acaatatgcc ttcggtttcg
     6241 acatcggcgc gttggatata aggaacatca acggtcaggc ggttttgggt tgcacccttg
     6301 ataaaggcat ccatcgtaaa cgcaccgggg gcgggtttgc caaaatcgct ataagccatc
     6361 gggccggcaa aactcatcat attgcctttt gccaacaatg ccagctggac ggcgcatacg
     6421 tcgctgaatc cgaaaaagag cgtgccgtgt tcgcgcatcc ttgcgccgag cgaagcaaaa
     6481 tcgatatgcg gcagaatccg cgccgcaccg taaccgccgc gcaaacccat cagcacttta
     6541 ggcgtggcga cgcggccgga agcgacctct tggaaatcgg cggcacgttg cgtgtccgtg
     6601 ccggcaaacc gttggaaacg gcggctgccc gcctgttggt tggttacggt aaaaccgaca
     6661 ttgtaaaggc gggttaaggc tgtgttgacg cggttggtgt cttcggcaaa tcctgacgaa
     6721 gcgacaacgc gcagaaggtt gccgtccgaa ctttgacggc gtggcgtttg gagaggcacg
     6781 gttcgggctt tcacaacgga atgggaagag ggaaggggcg gaacggatgt ggcggatgtg
     6841 ccgcaagcct gaagcagccc cgcgccggca gcggcggtgc aggttttcag aaaacggcgg
     6901 cgggaggtcg gttcggtcat aatttccctt tcatacggtt tcagacggca tcggcgggct
     6961 taaagcagct ttttaagctg ttccgtccaa tcttgcggca gggtcatacc gtgtttccgg
     7021 acggggtttg cccaaatcgg cgcggggaaa gacgcatcgt tttcaaagcg ggcgataata
     7081 tgccaatgca ggtgcggcac gacattgccc aagctggcga ggttgatttt tgccggccgg
     7141 aacacttggc gcatagcggc ttcgactttg tacaccattt ccatcaattc gccgcgttcc
     7201 gctgccgaaa ggtcggtcat ttcggcaata tgcttacgcc aaatgacgcg gcagaatgca
     7261 ggcgaaccgc tgtcgttatg gacggcgatg acgcggaggt tgggggtttg cagcaaaacg
     7321 tcttcatttt gcgccgtgca gatggggcag ggttgggcgg tcatttttcc ttattcctga
     7381 aagtgtttaa aaaacggtag ggtaatttct gatatagcgg attaacaaaa atcaggacaa
     7441 ggc
//
LOCUS       DAWIVB010000107         7383 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWIVB010000107 DAWIVB010000000
VERSION     DAWIVB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.107"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            21..1661
                     /locus_tag="WIX60_001459"
     CDS             21..1661
                     /locus_tag="WIX60_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ5305717.1"
                     /translation="MLPAFFAVLAATLAGGFFLFTKDDFFLLWKSALTALGFASNLYF
                     ARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVARKSLRVQFGFLAALCALS
                     LAASFIPSALDKYYLPHLRACEMLVGSLTAVWMRCRQPAVGRRCAAAGALFAVCILST
                     CLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSLYL
                     WHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYHCIEKPFKKWKGSFAQSVLWI
                     YALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVL
                     GDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASRVFQNWAACRAVYRYAEEH
                     LPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQAVYLMADNLASS
                     YNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPADFQ
                     IGGLPVYSDTDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            1723..3018
                     /gene="tyrS"
                     /locus_tag="WIX60_001460"
     CDS             1723..3018
                     /gene="tyrS"
                     /locus_tag="WIX60_001460"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ5305718.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGTKPEAQRILAEEMTRLIHGEEALVAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEISDGINAVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            3318..4238
                     /gene="ribF"
                     /locus_tag="WIX60_001461"
     CDS             3318..4238
                     /gene="ribF"
                     /locus_tag="WIX60_001461"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ5305719.1"
                     /translation="MKIRLGRHNAPDFPQGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQGLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAARRW"
     gene            4380..7169
                     /gene="ileS"
                     /locus_tag="WIX60_001462"
     CDS             4380..7169
                     /gene="ileS"
                     /locus_tag="WIX60_001462"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ5305720.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYLFKDTAA
                     LAAAFGLAGFEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGRYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQECLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1756 a         2113 c         1888 g         1626 t
ORIGIN      
        1 atatccgccg catcaagcgg attctgcccg catttttcgc cgtattggcg gcaacgctgg
       61 caggcggctt ctttttattc accaaagatg atttctttct tttgtggaaa tccgcgctga
      121 ccgccttggg tttcgcctcc aacctgtatt ttgcaagggg gaaggattat ttcgatcccg
      181 cgcaggaaga aaagcccttg ctgcacattt ggtctttgtc ggtcgaagaa caattttact
      241 ttgtctttcc gatactgctg ctgcttgtcg cccgcaaaag cctgcgcgta cagttcggct
      301 tccttgccgc actgtgtgcc ttaagccttg ccgcctcctt tataccttcc gcgctcgata
      361 aatattacct gccccacctg cgcgcctgcg aaatgctggt cggatcgctg accgccgtgt
      421 ggatgcgctg ccggcaacct gccgtcggca gacgctgtgc cgccgccggc gcattgtttg
      481 ccgtgtgcat attgtcaacc tgcctgtttt cctattcgga acaaaccgcc tatttcccgg
      541 gccccgccgc tttgattccc tgtctggctg ttgccgcgct gatttatttc aatcattacg
      601 aacacccgct taaaaaattt ttccaatgga aaatcaccgt tgccgccggt ttgatttcct
      661 attcgcttta tctgtggcat tggccgatat tggcctttat gcgctatatc ggcccggaca
      721 acctgccgcc ttattcgccg gcggcagcaa tcgtcctgac tctggcgttt tccctgattt
      781 cttatcactg catcgaaaag ccgtttaaaa aatggaaagg ttcgttcgca caatccgttt
      841 tatggattta tgccttgcct atgctcattt tgggggcggg ctcgtttttt gcgatgagac
      901 tgccgtttat ggcgcaatac gaccgcttgg ggctgacgcg ttccaacacc tcctgccaca
      961 acaataccgg caaacaatgc ctgtgggggg atacggaaaa acagccggag ctgctggttt
     1021 tgggcgactc ccacgccgac cattacaaaa cattcttcga tgccgtgggc aaaaaagaaa
     1081 aatggtccgc cactatggtt tccgccgacg cttgcgccta tgtggaaggc tacgcgtccc
     1141 gcgtgttcca aaactgggcc gcctgccgcg ccgtttaccg ctatgccgaa gaacacctgc
     1201 cccggtattc aaaagtggtt ttggcgatgc gctggggcag ccaaatgccc gaaaacagcc
     1261 gctcccttgc ctacgatgcc ggttttttcc aaaaattcga ccgtatgctg cataaactct
     1321 cgtccgaaaa acaagccgtt tacctgatgg cggacaacct tgcctcgtct tacaacgtcc
     1381 agcgcgccta tatcttgtct tcacgcatac cgggttaccg ccaagccctg cgcccggacg
     1441 acgaaagcac cctgaaagcc aatgcacgca tcagggaatt ggcagccaaa taccccaacg
     1501 tctatattat tgatgccgcc gcctatatcc ccgcagattt tcaaatcggc ggattgccgg
     1561 tttactcgga cacagaccac atcaaccctt acggcggcac ggaattggca aagcgttttt
     1621 ccgaaaaaca acggtttctc gatacgcgcc ataaccattg attcgcttaa atttgttaca
     1681 atcggcggtt tgcaaaaacg ctaatttttt tgaaagagac cgatgagcgt catccaagac
     1741 ctgcaatcgc gcggcctaat cgcgcaaacg accgacatcg aagccttaga cgctttgttg
     1801 aacgaacaaa aaatcgccct ttactgcggt ttcgatccga ccgccgacag cctgcacatc
     1861 ggacacctgt tgcccgtatt ggcattgcgc cgcttccaac aggcggggca tacgccgatt
     1921 gcactggtgg gcggcgcgac cggtatgatc ggcgacccca gcttcaaagc cgccgaacgc
     1981 agcttgaatt ccgccgaaac tgttgccggc tgggtggaaa gtatccgcaa ccaattaacc
     2041 cctttcttga gctttgaagg cggaaatgcc gccattatgg cgaacaatgc cgactggttc
     2101 ggcagcatga actgccttga cttcctgcgc gacatcggca agcatttctc cgtcaacgcc
     2161 atgctgaaca aagaatccgt caaacagcgc atcgaccgcg acggcgcagg catttccttc
     2221 accgagttcg cctactccct gctgcaaggc tacgactttg ccgagttgaa caaacgccac
     2281 ggcgcggttt tggaaatcgg cggctccgac caatggggca acatcaccgc cggtatcgac
     2341 ctgacccgcc gcctgaatca aaaacaagtg ttcggcctga ccctgccttt ggttaccaaa
     2401 tcagacggca ccaaattcgg caaaaccgaa ggcggcgcgg tgtggctgaa cgcaaaaaaa
     2461 acctcgccgt accagttcta ccaattctgg ctgaaagtcg ccgatgccga tgtgtataaa
     2521 ttcctgaaat actttacctt cctgtccatc gaagaaatcg atgccatcga agccaaagac
     2581 aaggcaagcg gcaccaagcc cgaagcgcaa cgcatcctcg ccgaagaaat gacccgcctg
     2641 attcacggcg aagaagccct tgtcgccgcg cagcgcattt ccgaaagcct gtttgccgaa
     2701 gaccaaagca gcctgaccga aagcgacttc gagcagctcg ccctcgacgg cctgcccgca
     2761 tttgaaattt cagacggcat caacgccgtc gaagccttgg taaaaaccgg tttggcatcc
     2821 tccaataaag aagcgcgcgg ctttgtgaac agcaaagcgg ttttgctcaa cggcaaacct
     2881 gccgaagcca acaaccccaa ccacgccgcc gaacgccccg acgatgcctg cctgctgaac
     2941 ggcgaacaca aacgtttcgg caaatacacc atccttcggc gcggcaaacg caaccacgcg
     3001 cttttggttt ggaaataatc cgattgccgc agaaatgccg tcattcccgc ctgcgcggga
     3061 atgacggcgg agggttgctt gtctcggttt acctggttaa aaaagaacga ttttcactga
     3121 tgttgcatca ggtttggggc gatgtttcaa cacatagcac cgcgcctgct gcgcgttttt
     3181 gtgcgtttgg cgcgttcggc ggcgggaaat ttgcctactt ttcccgcgtc gggcgggcgt
     3241 aacgggcggc acactgtcta taaaccgcaa taccgtttac aatgaccgcc tgtttcacca
     3301 catacccgaa cgcaacaatg aaaatcaggc tggggcggca caacgcgccc gactttccac
     3361 agggtgccgc cgtaaccata ggcaatttcg acggcgtaca cctcggacac aaacacatcc
     3421 tccaaaaact ccgcctcgaa gccgacgcgc gcggactgcc cgtcgtgacc gtcgttttcg
     3481 aaccccaacc caaagaattt ttcgcactcc gcaccggcag gatgccaccg tgtcggatca
     3541 gccccctgcg caccaagctc gaattattgg aaggcacagg ctgtgtcgat gccgtctggg
     3601 ttttgcgttt cgatcaaaat ttttccgaaa tatccgcgca agggtttatc gaccgcctgc
     3661 tgcgtcaaac cttgaatacg cgttatttgc tcgtcggcga tgatttccgt ttcggtgcgg
     3721 ggcgggaagg ctgttttgaa cttttggcac aacagcccga tatgcagacc gagcgtacgc
     3781 cttccgtcat cgtcgaagac atccgcacca gcagtaccgc cgtgcgacaa gccctttcag
     3841 acggcaacct tgcctatgcg aaaaaacttt tgggacacga ctacgtcttg agcggcaggg
     3901 tggtgcacgg cagaaaactc ggacgcacct taaacgcccc gactgccaac atccgcctgc
     3961 cccgccaccg ttatgcactc ggcggcgtgt ttgtcgtcga agcagacggc gcattcggca
     4021 cgcggcgcgg cgtggcgagt ttcggcttca atcccaccgt tgatagcggc tgttctcaaa
     4081 agcttgaagt ccacctgttc gactttcaag gcgacctgta cggacaaggg ctgaacgtcc
     4141 gcttcctgca caaactgcgc gatgaggaaa agtttgacgg tatggaagaa ctgaaaaggc
     4201 agattgaagc cgatatggaa gccgcaaggc gttggtagaa aaaccttata caaaccatcc
     4261 gattgggcta caatcaatct tttaactgtt cagacggcac agggttttcc cgttgtgaaa
     4321 tgctgtttgg ggcgcaatgc cgtctgagac cgaaatattg taacaataga gattaaaaaa
     4381 tgaccgatta cagtaaaacc gtaaacctgc tcgagagccc gtttccgatg cgcggcaatc
     4441 ttgccaagcg cgagcctgca tggctgaaaa gctggtacga gcaaaaacgc taccaaaaac
     4501 tgcgcgaaat cgccaaaggc cgtccgaaat ttattctgca cgacggcccg ccgtatgcca
     4561 acggcgacat ccacatcggt catgccgtca ataaaattct caaagacatc attatccgca
     4621 gcaaaaccca agccggtttt gacgcgcctt atgtgccggg ttgggactgc cacggcctgc
     4681 ccatcgaagt gatggtagaa aaactgcacg gcaaagatat gcccaaagca cgtttccgcg
     4741 aattgtgccg cgaatacgcc gccgaacaga ttgcccgtca gaaaaaagac tttatccgct
     4801 tgggcgtgtt gggcgactgg gaccatcctt acctgactat ggatttcaaa accgaagccg
     4861 ataccgtgcg tatgctcggc gaaatctaca aatccggcta tctctaccgg ggtgcgaaac
     4921 cggttcaatt ctgcttggac tgcggttctt cgctggccga agcggaagtg gaatacaaag
     4981 acaaaatctc gcccgcgatt gacgttgcct atctgtttaa agacactgcc gcgcttgccg
     5041 ccgcattcgg tttggctggt ttcgaaggca aagcgtttgc cgtcatttgg acgactacgc
     5101 cttggacgct accggcgagc caagccgtgt ctgcgggtgc agacgtggtg tatcaactga
     5161 ttgatacgcc gaaaggcaaa ttggtattgg cgaaagattt ggcagaagac gcgctcaaac
     5221 gttacggttt ttcagacggc attgctattc tcgccgaaac caccggcgac aagctggaaa
     5281 atctgcacat gaaccatccg ttcctcgaac gcgatattcc catgctcaac ggcgaacacg
     5341 ttaccaccga tgccggtacc ggcttggtac acaccgcccc cgcgcacggt ttggaagact
     5401 acgccgtctg caataaatac ggcatcgagc tttacaaccc cgtcaacgcc gaaggccgat
     5461 acatcggcga aacgccgcgt gtcgccggta tgcgcgtttg ggaggcgaac cccgtcatcc
     5521 tgcaatggtt ggaagaaacc ggcaaccttt tggcaagcag taaaatcgaa cacagctacg
     5581 cccactgctg gcggcacaaa acgccgctga tttaccgcgc gacaggtcaa tggtttgtcg
     5641 gtatggacaa agccggtgcc gacggcaaaa ccctgcgcga caaagccatc aaggccgtgg
     5701 acgacaccga attcttcccg tcttggggtc gcgcgcgttt ggaagccatg attgaaggtc
     5761 gtcctgactg ggtggtttca cgccaacgct attggggcac gccgatgact ttctttgttc
     5821 acaaagaaac gggcgagctg catccgaact ctgccgaact tttggaaaaa gttgccctga
     5881 aaatcgaaga aaaaggcatc gaagcgtggt tctccctcga taagagcgaa ctcttgagcg
     5941 cggaagattg cgaaaattac gataaacttt ctgacacaat ggacgtatgg ttcgactccg
     6001 gctcgaccca ttattccgtt gtgaaacaac gcgaagaatt ggaatggccg gctgatttgt
     6061 atctcgaagg cagcgaccaa caccgcggct ggtttcaatc gtccatgctg accggctgcg
     6121 cctcatcaat gggtcgcgcg ccgtataaac agctgctgac ccatggtttc gttgtcgacg
     6181 gcgaaggcaa aaaaatgtcg aaatccatcg gcaacgtcgt tgcaccgcaa gaggtttata
     6241 acgaattcgg cgcagacatc ctgcgcctgt gggcggcatc taccgattac agcggcgaat
     6301 tggcgatttc caaagaaatc ctcaaacgcg taaccgaaag ctaccgccgt atccgcaata
     6361 ccttgagctt tttgtttgcc aacttgagcg actttaatcc gattgaagat gccgtgcaac
     6421 aggcggatat ggtggaaatc gaccgctacg ccgtggtatt ggcacgtcag ctgcaagagt
     6481 gtctggcagg cgattactat ccgcgttatg ccttccactt tgccgtaaaa gacattgttt
     6541 ctttctgctc ggaagacttg ggtgcgttct acctcgacat cctgaaagac cgcctctaca
     6601 ccaccaaagc agacagccat gcacgccgca gcgcacaaac tgccctgtat cacatcacac
     6661 gcagtttggt tctcttgatt gcaccgattt tgtgcttcac cggcgaagaa gcgtgggaca
     6721 tcatcggcgg cggcgaagaa gacagcgtcc tcttccatac ttggcacgag ttcccgacca
     6781 tcaacgaaaa aaccgaagcc gaactggtga aaaaatggac ggcaatccgc gaagcccgcg
     6841 aagcggtaac cgccgccatc gagcctttgc gcgccgacaa aaccgtcggt tcgtccttgc
     6901 aagccgaagc cgaaattacc gcgccggaag aaatggccgg ctatctgaat gctttgggcg
     6961 aagaattgcg ctttgctttg ctggtgtcta aagcagaagt gaaagtaggc agcgaacttg
     7021 ccgttgccgc taaagccagt gatggtgaaa aatgcgaacg ctgctggcac tacacccgcg
     7081 atgtgggcgc ggttgcaggc tatgaaaccg tctgcaaacg ctgtgcagag aatgtcggcg
     7141 gagaaggcga aacgcgccat tacgcctgat aaagtttgag caaatgccgt ctgaaaccgc
     7201 cgaacagcat ttcagacggc attttttgtg ccgcgatttg tctttataac ggcggagggg
     7261 tttcaagatt gcggtgttgt cggaatgcaa ctgaaccgtc attcccacga aagtgggaat
     7321 ctagaatctc gaggtttcag tcatttccga tagattcccg cctgtgcggg aatgacggat
     7381 ttc
//
LOCUS       DAWIVB010000108         7339 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWIVB010000108 DAWIVB010000000
VERSION     DAWIVB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.108"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            66..260
                     /locus_tag="WIX60_001463"
     CDS             66..260
                     /locus_tag="WIX60_001463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305721.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            431..955
                     /gene="nspA"
                     /locus_tag="WIX60_001464"
     CDS             431..955
                     /gene="nspA"
                     /locus_tag="WIX60_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ5305722.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1091..1480
                     /locus_tag="WIX60_001465"
     CDS             1091..1480
                     /locus_tag="WIX60_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ5305723.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1558..2388
                     /locus_tag="WIX60_001466"
     CDS             1558..2388
                     /locus_tag="WIX60_001466"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ5305724.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2403..3860
                     /locus_tag="WIX60_001467"
     CDS             2403..3860
                     /locus_tag="WIX60_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ5305725.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3901..4290)
                     /locus_tag="WIX60_001468"
     CDS             complement(3901..4290)
                     /locus_tag="WIX60_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305726.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDIPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(4472..4873)
                     /locus_tag="WIX60_001469"
     CDS             complement(4472..4873)
                     /locus_tag="WIX60_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305727.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(5290..5619)
                     /locus_tag="WIX60_001470"
     CDS             complement(5290..5619)
                     /locus_tag="WIX60_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305728.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVIPLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(5645..6352)
                     /locus_tag="WIX60_001471"
     CDS             complement(5645..6352)
                     /locus_tag="WIX60_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ5305729.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKSYNLTVADWHTYFVKGSQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSV
                     KSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLNKTQTFNNGSAEYHGHVRSW
                     QDLHTDQKNALKKAGLD"
     gene            complement(6487..6804)
                     /locus_tag="WIX60_001472"
     CDS             complement(6487..6804)
                     /locus_tag="WIX60_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305730.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(6807..>7339)
                     /gene="mafB"
                     /locus_tag="WIX60_001473"
     CDS             complement(6807..>7339)
                     /gene="mafB"
                     /locus_tag="WIX60_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ5305731.1"
                     /translation="DQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDF
                     SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKST
                     LNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKN
                     GVHIVPANPKTIKKVQ"
BASE COUNT         1820 a         2024 c         1513 g         1982 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaacc gtatgcctgc acctgcaaca
      181 atccgacaga taccgctgtt ttttccaaac cgtttgcaag tttcacccat ccgccgcgtg
      241 atgccgccgc caccatttaa aggcaacgcg cgggttaacg gctttgccgt cggcaaagca
      301 gccggatacc gctacgtatc ttgaagtatt gaaaatatta cgatgcaaaa agaaaattta
      361 agtataatac agcaggattc tttaacggat tcttaacaat ttttctaact gaccataaag
      421 gaaccaaaat atgaaaaaag cacttgccac actgattgcc ctcgctctcc cggccgccgc
      481 actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg caaaagcctc
      541 aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct accgcatcaa
      601 cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc catccaccga
      661 tttcaaactt tacagcatcg gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt
      721 caaaccgtat ctcggcgcgc gcttgagcct caaccgcgcc tccgtcgact tgggcggcag
      781 cgacagcttc agccaaacct ccatcggcct cggcgtattg acgggcgtaa gctatgccgt
      841 taccccgaat gtcgatttgg atgccggcta ccgctacaac tacatcggca aagtcaacac
      901 tgtcaaaaac gtccgttccg gcgaactgtc cgccggtgtg cgcgtcaaat tctgatatgc
      961 gccttattct gcaaaccgcc gagccttcgg cggttttgtt ttctgccacc gcaactacac
     1021 aagccggcgg ttttgtacga taatcccgaa tgctgcggct tctgccgccc tattttttga
     1081 ggaatccgaa atgtccaaaa ccatcatcca caccgacaaa gcccccgccg ccatcggcgc
     1141 gtacagccaa gccgtccgag caggcgacac cgtttacatg agcggtcaaa tccccctcga
     1201 tcccgccacg atgaccgtcg tcggcaacgg cgatttccgc gccgaagcgc gccaagtgtt
     1261 ccaaaacctg caagccgtcg ccgaagcggc aggcggcacg ctggccgaca tcgtcaaact
     1321 caacgcctac ctgaccgact tgggcaattt tgccgtcttc aacgaagtca tggcggaatt
     1381 tatcgccgag cccttccccg cccgcgccgc cgtcggcgtt gcctcgctgc ccaaaggcgt
     1441 gcaggtcgaa gccgaagccg tcctcgtttt gaacgcataa ttccacgttc ggactgcatg
     1501 atatttcaaa accatgccgt ccgaaccgca acactttccc acttcacaac atctgcaatg
     1561 gcacattacg caatcggcga catacaaggc tgtttcgacg aactgaccgc gctgctcggc
     1621 aaaatcggtt tcaaccacgg cacggacacc ctctggctga cgggcgacat cgtcaaccgc
     1681 ggcccgaaat ccctcgaaac gctgcaattc tgcatccggc acgaaaacag cgtgcaaatc
     1741 gtcctcggca accacgacct gcacctgctc gctgtcggct gcggcgaagg tgcgcccaaa
     1801 cgcagcgaca caatcgaacc catactcaaa caccccgacg gaaaaaaaat gctcgactgg
     1861 ctgcgtgcgc aaccgctgtt gatacgcgag ggcagccgcg tgatggtaca cgccggcatc
     1921 ctgccgcaat ggcgcataac caaagccgaa tcgctcgcta gagaagccga agccgaactg
     1981 cgcggcaaaa aatacgtcaa attcttctcc aaaatgtacg gcaacaaacc ggccgcgtgg
     2041 gacgaaggtt tgaaaggcta tgcccgcctg cgcttcatcg tcaacgcctt cacgcggatg
     2101 cgcgccctga cctttaaaaa cgaactggat ttcgactaca aatccacagt gaaaaaaatg
     2161 ccgccttacc tgcgcccgtg gttcaaagcc cccgaccggc aaaacctcga ccgcaccatc
     2221 atcttcggac actggtcctc gctgggctac acgaatgccg acaacgtcat ctcgctggac
     2281 accggcgcgc tgtggggcgg acagctgacc gccgtcaacc tcgaaacgga agaaattacc
     2341 caagtccaag ccgccggcgg catagactgg aaaagcttcg caaaataacg gaccgcccca
     2401 ctatgcacaa aatcctgccc atcgcccacg tcctctcccg actgggtatg ctgttttcct
     2461 ttatcctgct gatacccgcc gccctctcct acgccttttc ggacggcgcg tacaccgcct
     2521 tcgccaccac cgcgaccgtt accctttccg gctcgtgcat cgtccggctc gccaccctcc
     2581 ggttcaggcg cgaactgcgc ccgcgcgacg gctttaccct cgtcctgatg ttgtggctgg
     2641 cgtttgccgc tatggctgcg atgccgatgt acctgtattt cccgaatatg ggctttaccg
     2701 acgcattttt tgaatcgatg tcgggactga ccaccaccgg cgcgaccgtc atcccccacg
     2761 tcgacgggct cgccccctcc gtcaactttt ggcggcatat gctcaactgg ctgggcggga
     2821 tgggcatcat cgtccttgcc gtcgccatcc tgcctatgct cggcgtaggc ggcacgcagt
     2881 tattcaaagc cgaaatcccc ggcattgaca aagaaagcaa aatgtcgccg cgcatttccc
     2941 aagtggcgaa aaaactttgg ttcggctaca ccctgatcac catcctcgcg gcagcctgcc
     3001 tgcattttgc cgggatgggc tggttcgatg ccgtctgtca cgcgatggct accctctcgc
     3061 tgggcgggtt ttccacccac gatgccagca tagcttatta caactccccc ctcatcgaag
     3121 cggtcatcat cgttttcacc attgtcggcg gcatcaattt tgccaaccat ttcgccgccc
     3181 tcaacagccg cagcctcaaa acctattgga aagacgaaga atgccggacg atgctgctgc
     3241 tgctctccgg cagcatcctt gccgccgccc tgtacctgtg gcacaccggc tattacgccg
     3301 gttttaccga atccctgcgc tacaccgcct tcaacttcgt ctccatcgga ctggcaaacg
     3361 ggctggccaa caccgacttc gcacaatggc cgctcctgat ttccctgtgg atgtttttcc
     3421 tcgccaacat cctcgccaac tccggctcga ccggcggcgg catcaaaacc atacgcgccc
     3481 tcgtcctgtt caaattcagc ctgcgcgaaa tgatggtgct gctgcacccc aaagccgtcc
     3541 gcaccgtcaa aatcagcggc aaggccatac ccgaccgcct cgcgctgacc gttatgtcct
     3601 tcatcttcat ctactttatg accgtcgtcc tcttcagctt cctgctgatg gcgagcggta
     3661 tggaatttac caccgccttc accgccgtca tcgcctgcat caccaacgcc ggccccggcc
     3721 tgggcgaagt cgggcccgcc ggcaattacg ccggtttgga cgtgatgcag aaatggatct
     3781 gcgttaccgc catgctcttg ggcaggctgg aaatcttcac cgtcttcatc ctcttcaccc
     3841 ccgcctactg gaagaaataa aaaatgccgt ctgaaaactt ttcagacggc atattgtctt
     3901 ttaaaaaagc ggaagctcta caaaaggaaa gaaatgctga aacctctgat aaatttcagg
     3961 atatttctct aaggctttta atgctttttc ttttgaaata ggcggatcat agacatctat
     4021 cccccttaag aaggcaatgc ccgttaatgc atttttttga tcatttgaaa tgtaaccata
     4081 attaacgact ccaattacat cataaatttc ccctatactc ttatcaaaat caatcaaatc
     4141 aaaaatatac aatggaattg tatcaatagg aatatctctg acaactatat ccaaccataa
     4201 tttaaattca gaaagattaa tagcatcata gaacatacag actattgcaa atcctaaatc
     4261 ttcacaattt tctttactaa ttttccacat aattatttac ctttatccat tgcttcccaa
     4321 ccgatacatc cggtatttta gcttctcctt ttccgtcaaa cactcctttg gttacctgct
     4381 ttgtgtcaaa aataccgtct gaaagccgaa tatcgtttca gacggtattt tgattgttgg
     4441 gtttttaatc agagctttgt ttgataattt gttattttga ataaaataca ggttctgtcg
     4501 agctgataac ttctattaca gaatcgctaa aaaagaaaat ataagcctta taatatttct
     4561 tttcaaaaaa atcaaaactt tcttctttca accagtccat ataactatca tctattatct
     4621 cgaaaaaata atttatagca ggatttctat cccaatcatt tttttcttta ttttcaaata
     4681 aatattctga aaattttact ttataagatt catccgttat ttgaaaatga attatattgt
     4741 caataaatat ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt
     4801 gttcggtaac acaaattttg tccacataaa atctgtgatt attgttagaa aattgtaatg
     4861 gtttaatctt cacaatcgtt ttccatatct aatttttgtt ttcttattgc cctgccggat
     4921 aaagcaggtc tgcctccgta ctgtctatcc tcacatttac tttcctgcca tcggctttta
     4981 tctccccggt tttaccgttt ttgccaactt tgcggctttg gcggcggcaa tgttgaaggc
     5041 ggcttcgacg gtttcggcgg cattggggtt gacccgtact catccgcaaa cggcaacacg
     5101 acggacggtg tttgcttttc agccgtctgc cgttttgacc aaggtgctgc cgtggaagga
     5161 gcaagtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaat gaaagaaatg
     5221 ccgtctgaaa attaaacacg gtttcagacg gcattttgat tgttgggttt tcaacccgac
     5281 ctacggttcc taatgatttt cagataaaaa taaagaaata tcttctaacg catcttcagc
     5341 cctaccttga agctctggat catcttgctt tgaatgtagt aaaggaataa ctttatcgta
     5401 atcaccgata tttgaatgta gcctggcgag atgtccgaaa caggttagcg ataatccggc
     5461 aatatccaag tctttatttt tcgataattc catcaatttc tcagaaatcc acggtacttc
     5521 atatgctttg gaattaaata ccatatcaag aatgccatct ataacacttt gtttatcatc
     5581 agaagaaatc aacttttctg ttttactttt atcagtcatt ttttaatttt tccttttgaa
     5641 ttaactaatc caatcctgct ttttttaaag catttttctg atcggtatgc aaatcttgcc
     5701 aacttctgac atgcccgtga tattccgcag aaccgttatt aaaagtttga gttttgttta
     5761 atacaacgat ttcattattg gctttatcaa ccccaactct tcgaggagaa gttgatttaa
     5821 cttgaacgga attatcaaga gctgcttgtc cgtttgttgg tgctctactt ttcacagaat
     5881 tatcattttt gccatgataa gaagcgtctt tatatcgttg gttgccttta tcatacggac
     5941 aatcattatg aacccaaacc ccttccgttt ccgcctgact gcccttgacg aagtaggtat
     6001 gccaatcggc gacggtcaga ttgtaggatt tgagcggctg ctgtttgaag gtaatgtttt
     6061 gaaccgtctg ttttgcgccg ctttcggaaa gcagggtgtc gccttttttc agacgacctg
     6121 cctgtatcca ttttccttga ctgtaaaacg ggtggatttt attggaaatc agggtttggt
     6181 tgttgccgat gccgtctgaa atttcaatgt aaacggtttc ttgatacgga ttgccgtatc
     6241 gggcggtaac gggtttgtat cccgtttttc cgcttgcctc gtccttggcg aagacgcggt
     6301 cgccggttcg gatacgggca atggctttgt agccgtctgc cgttttgacc aaggtgctgc
     6361 cgtggaagga gcaggtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaat
     6421 gaaggaaatg ccgtctgaaa attaaacacg gtttcagaag acattgtgat tgttggtttt
     6481 tttaacctac gcttgcgaaa ttaattcctt tctcctgaat actgcaccat ttatttggtt
     6541 taactccata gaagaatgat tagaataaat acattcaaag tcagcttcat caaaatattc
     6601 tccacacata gaccataact ctgtaatcaa atattccata tcttcatggt cttctgtgat
     6661 ggcttctctt gataaataat ttttgatttt taaaaacctt cttccttcct cgtaatctac
     6721 ggtaattaac ctgatatctg agtgtatcat gcccagcatt gctatattca taccgactcg
     6781 cagccagctt ggtaatatat tcataactat tgcacctttt taatggtttt agggttagcc
     6841 ggaacaatgt gaactccatt tttacctgaa tgaatcgtac caaaattggt acttaatcct
     6901 gatttcccat cgctgcctat aggataccca aaatcaacaa caggatttcc tcttgctcct
     6961 ttagaaataa ctggataagc acctgaatgt attccgttca acaattcttg aggattaatg
     7021 ttttgattta aagtactctt accttcaatg tagtttctat gtcctgaaat atgtttccct
     7081 tgagctccat catgaatttt agtcccaata gattttgctt taaaatcaac attgcgggtt
     7141 tgaaacattt ctccatctac agactgagat aatcttgaag cagtgttatg cttgtaggag
     7201 tctgagaaat ccccactaac cgcagccttc cccggttttg ccgcctttgt caggtttttg
     7261 actttggcaa acggcaggac gttgaccagg gcttcgacgg tttcggcggc attggggttt
     7321 tcctgcatcc actggtcaa
//
LOCUS       DAWIVB010000109         7225 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWIVB010000109 DAWIVB010000000
VERSION     DAWIVB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.109"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(125..452)
                     /locus_tag="WIX60_001474"
                     /pseudo
     CDS             complement(125..452)
                     /locus_tag="WIX60_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(481..810)
                     /locus_tag="WIX60_001475"
     CDS             complement(481..810)
                     /locus_tag="WIX60_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ5305732.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(888..1106)
                     /locus_tag="WIX60_001476"
     CDS             complement(888..1106)
                     /locus_tag="WIX60_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ5305733.1"
                     /translation="MLKTLTFAALHFSVAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVKQTQPFQLHRCS"
     gene            1228..2181
                     /locus_tag="WIX60_001477"
     CDS             1228..2181
                     /locus_tag="WIX60_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ5305734.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2256..4313)
                     /gene="metG"
                     /locus_tag="WIX60_001478"
     CDS             complement(2256..4313)
                     /gene="metG"
                     /locus_tag="WIX60_001478"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ5305735.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHACH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGTKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRDTLGKHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(4429..6267)
                     /gene="glmS"
                     /locus_tag="WIX60_001479"
     CDS             complement(4429..6267)
                     /gene="glmS"
                     /locus_tag="WIX60_001479"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ5305736.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGLYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKDAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPSDVEIASEYR
                     YRSVIADSDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            6549..7070
                     /locus_tag="WIX60_001480"
     CDS             6549..7070
                     /locus_tag="WIX60_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305737.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKIHVVTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYRDARNALPSNQTYQQHLAAIEQLKRRFEAEFDELEK
                     EIKCNGRSPALLL"
BASE COUNT         1521 a         1728 c         2151 g         1825 t
ORIGIN      
        1 cgactgtaac ttccttaaca tattttcctt tgattttaga ggaggatgcc acgccggcgg
       61 catcattaaa tcccgtcatt cccacgaaag tgggaatcta gaactcagga ccggagaaac
      121 ctttttaccc gataagtttc cgtgccgaca gacctggatt cccgcctgcg cgggaatgac
      181 gaagtttttc ggccattcgc cgtgattcgg gcaatactcg ggtgttttgt gcaaacggca
      241 gatgctgcgt cgcggcaggt tgacggcagg tgcttggtgt caatcttctt accgttggcg
      301 gcggcggcgg cggtaacgtc gtcgttggcg gctttggcgt tgtcgcgctc aaccggctgt
      361 ccgcagaacc attttaccga accggcttga cgcttggccc acagggagag ttttctgcct
      421 ttgatttctt tgtttacgcc gcttgaagcc attatgtcag acggtattgc ccgggcagct
      481 ttattcgtac actttcagca gctcgacttc aaatatcaaa gtggcgtgcg ggggaatcac
      541 gccgcccgcg ccgtgtgcgc cgtagcccat ttccgaaggg atggtcagct tgcgtttgcc
      601 gccttccttc atgccgccga agccttcgtc ccagcctttg atgacttgtc cgacaccgag
      661 cgtgatggtc agcggctggc ggcggtcgag gctggagtcg aatttggttc cgttttccag
      721 ccaacctgtg taatgcacgg taatctcttt gcctttaact gcttcttttc cgaagccttc
      781 ttgcaagtct tcaataatca ggccgcccat atttgtcctt tcgttgcttg ttggtcaaaa
      841 cggcaagggt aacataccgt ccgtcgaagt caaatgccgc tcaaacgtca gctgcatcgg
      901 tgcagctgaa acggctgtgt ttgtttgact gttttatttt tttcgtaaag gttccatgct
      961 ttttcatgga aatagaaaac gacggtgttg attaggggtt cgaccagcgc aactgctccc
     1021 gatacgccta tactgcccgt cagtacatag gttacactga aggcgacgct gaaatgcagt
     1081 gcggcaaaag tcagggtttt aagcatcatc ctctcccgga ttggacattg acggagagat
     1141 gataaagatt atcataaggc tgcgcggttt aaatttgcta tttgttgtta gtgtagataa
     1201 atcgtttttt aaataaggat aggaattatg aatcataaaa agatcgttgt tttggatgcg
     1261 gatactttgc ccggccgggt ttttcatttt gattttccgc acgagcttgc ggtttacggt
     1321 acgacaggtg cggatgaaac ggcagaacgg gtgcgcgatg cacatattgt cattactaac
     1381 aaagtgatga tttctgccga tattattgcg gctaatccgc agttggagct gattgccgtc
     1441 agtgcgaccg gcgtgaacaa tgtcgatatt ggggcggcga aggcggccgg tgttgcggta
     1501 tgcaatgtcc gcgcatacgg aaacgaatcg gttgcggaac acgcctttat gctgatgatt
     1561 gcgttaatgc ggaatttgcc tgcctatcag cgtgatgttg cggcaggatt gtgggaaaag
     1621 tcgccgtttt tctgccatta cggcgcgccg attcgggatt tgaacggcaa aacgctggcg
     1681 gttttcggac gcggcaatat cggacggacg cttgccggat acgcgcaggc attcggtatg
     1741 ggggtggtgt ttgccgaaca caaacacgcg tccgctgtgc gtgaaggcta tgtttccttt
     1801 gaagatgcgg tacgggctgc tgatgtgttg tcgctgcact gtccgctaaa cgcccaaact
     1861 gaaaatatga taggcgaaaa cgaattgcgg cagatgaagc ctggcgcggt tttaatcaat
     1921 tgtgggcgcg gcgggctggt ggatgaaaac gcgctgcttg ccgcactcaa atacgggcag
     1981 atcggtgggg caggtgtcga tgttttgacg aatgagccgc ccaaaaacgg caatcccttg
     2041 ctgaatgcac gattacccaa tctgattgtt acgccgcata ccgcgtgggc aagtcgtgag
     2101 gctttggaca ggctgtttga tatattgttg gcgaacattc acgcctttgt gaaaggagag
     2161 gcgcaaaacc gcgtggtttg aacctgtcgg gattgcggaa aaaaatgccg tctgaacgcc
     2221 tcaagggttc agacggcatt tcttgagatt cccgtttaac cgactttgtc gcccggctgc
     2281 gcgcctgtat ccacatccaa gagcttcagt ttcccgtctg ccgtggcggc actcaaaatc
     2341 atgccttcag atacaccgaa ttttgccatt ttgcgcgggg cgaagttggc gacggcgatg
     2401 accatgcggc cgttcaattc ggcagggttc gggtaagacg cggcgatgcc ggagaagatg
     2461 atgcgttttt caaaaccgaa atcgaggtcg aatttcaaaa gtttggtgct gccttcgaca
     2521 gcttcgcagt tcaatacttt ggcaacgcgc atgtcgattt tcataaagtc gtcgaaactc
     2581 gcctgttcgg cgactttttc gtatttgccc tcttcggcgg caggtgcggc tgcggcggcg
     2641 atgctttgtt tgttggcttc gattaaatcg tccacttgtt tttgctccac tcgttgcatt
     2701 aaatgttcgt atttgttgat ggcgtgtttg cccaaggtat cgcgtgtatt tgcccaagtg
     2761 atggcttcca aattcaggaa tttggcggcg tttgcggcgg tttgcggcaa gacgggggcg
     2821 aggtaggcgg tcaacatggt gaaggcgttg atgagttcgc tgcatacttc gtgcaggcgt
     2881 tcgtcttggc cttcttgttt ggcgagttcc cacggcttgt tggcatcaac gtattcgttg
     2941 acaatgtctg ccaaggccat gatgtcgcgc agggctttgg cgtattcgcg gctttcgtag
     3001 cattcggcaa tggcttcgct ttgcgcagtc agttttgcca gcaatgcgct gtcggcaaca
     3061 tctttcagac ggccttcaaa gcgtttggcg atgaaacctg aggcgcgggc ggcgatgttg
     3121 acgtatttgc cgacgaggtc gctgtttacg cggctgataa agtcttgcag gttcaaatcg
     3181 atgtcttcga ttttgctgtt gagtttggcg gcgatgtagt agcgcatcca ctcggggttc
     3241 aggccttgtt ccagatagga tttggcggta ataaacgtgc cgcgcgattt ggacattttt
     3301 tgtccgtcga cggtcaaaaa gccgtgtgcg tacacgccgg tcggggcgcg gtggccggag
     3361 aaatgcagca tagcgggcca gaacagggcg tggaaataga gaatatcttt gccgatgaag
     3421 tggtacatct cggtttggct gtcggctttg aagtattcgt caaaatcgac gccgatgcgg
     3481 tcgcacaggt ttttaaacga cgccatgtag ccgacgggcg cgtccagcca gacgtagaag
     3541 tatttgcccg gcgcgtcggg gatttcaaaa ccgaaatacg gcgcgtcgcg ggaaatatcc
     3601 cagtcggaca gggtggtttc ttcaccttcg cccagccatt ctttcatttt gttgagggct
     3661 tcggcttgca gatggggctt gccgtcgtgc gggttgttgc cggaagtcca tgctttgagg
     3721 aagtcggcgc attcgcccag tttgaagaag aagtgttcgg attcgcgcaa ttcgggtttc
     3781 gtaccggaaa cggcggaata cgggttaatc agttcggtcg gggaataggt cgtgccgcag
     3841 acttcgcagt tgtcgccgta ttggtcttgg gcgtggcatt tcgggcattc gcctttgacg
     3901 aagcggtcgg gcaggaacat ttgtttttcg gggtcgaaaa gctgctcgat gacgcggctc
     3961 tcaatcttgc cgttggcttt cagcgcgcgg taaatgtctt gggaaaactg tttgttttca
     4021 ggggaatggg tgctgtaata attgtcgtaa ccgatgaaaa agccagtaaa gtcggcgagg
     4081 tgctcttcgc gcactttggc aatcatgtct tcgggcgcga taccttgttt ttgcgcggca
     4141 agcattacgg gcgtgccgtg ggtgtcgtcg gcgcagcagt agtggcacgc gtggccgcgc
     4201 agtttttgaa agcgcaccca aacgtcggtt tggatgtgtt cgaccatgtg gccgaggtgg
     4261 atgctgccgt tggcataggg cagggcggag gtaactaaga ttttgcgtgt catattgtgc
     4321 tttgcaaaca atgggtaaag gcggattata ccgcaaatca aacggggaaa tgccgtctga
     4381 agcctgaaaa atcgggcttc agacggcatt tttgccaacc ggcgggagtt attcgacggt
     4441 tacggatttc gccaggttgc gcggcttgtc cacatcggta ccgcgtgcga gggcggtgtg
     4501 gtaggcgagg agctgcacgg ggatagtatg cacgacgggg gacagtttgc cgacgtggcg
     4561 cggtgcgcgg ataacgtgca caccttcggt ggcattaaaa ttgctgtcga ggtcggcaaa
     4621 gacgaaaagt tcgccgccgc gcgcgccgac ttcctgcata ttggctttga ctttgtccaa
     4681 caggctgtcg ttgggtgcga tgacgacgac gggcatattt tcgtccacca gggcaagcgg
     4741 cccgtgcttc agttcgccgg caggataggc ttcggcgtgg atgtaggtga tttccttcag
     4801 cttcaacgca ccttcgaggg caatcgggta atggatgccg cgccctaaaa acagcgcgct
     4861 ggttttcttg gcaaactgtt gcgcccatgc ggcaatttga ggttcgaggt tcagagcgtg
     4921 ctgcacgctg ccgggaagct ggcggagttc ttcggtgtaa cgcgcttcgt cttcttcgga
     4981 aaccaaaccg cgcactttcg ccagcgttac cgccaaaccg aacagcgcaa ccagttgcgt
     5041 ggtaaacgct ttggtcgagg cgacgccgat ttccgcaccg gcacgggtat aaagcacgag
     5101 gctgctttcg cgcggcaggg cggattccat cacgttgcaa atggagaggc tgtggcggtg
     5161 tcccaaggat ttggcgtatt tcaacgcctc catcgtgtcc agcgtttcgc cggattggga
     5221 aatggtaatg accagttggt cggaatcagc aatcacgctg cggtatcggt attcgctggc
     5281 gatttcgacg tcggacggga tttttgcgat ggattccaac caatatttgg cggtcagcgc
     5341 ggcgtaatag gacgtgccgc aggcaaggat tttgacgctg cggatgcttt caaacacgct
     5401 tttggcatct ttgccgaagt tttcggggat gaagccgccg tcgaggaaaa cctccgccgt
     5461 gtctgcaatc gcgcggggct gctcgtggat ttctttttgc ataaagtggc tgtacagtcc
     5521 cagttccaaa gaggcgagcg agagttcgga taccttgact ttgcgttcgg caggcaggcc
     5581 gtttttatcg gtcagccttt tgatgccgtc tgaagccagc agcgcgatgt cgccgtcttc
     5641 gaggtacgcc acgcggcgcg taaaggcgat gacggcggat acgtccgaag cgataaaggt
     5701 ttcatcgtcg cccaaagcga ccaaaagcgg gcagcccata cgcgccacaa ctaattcatc
     5761 aggcttgtct tgggcaataa ccgcgatggc gtatgcgccg tggaaacgtt tgaccgcttc
     5821 ttgtaccgct tcaaacagcc tgccgccgtt ttgcgcgtat tcgtgattga tgctgtgtgc
     5881 gatgacttcg gtatccgttt gcgattcaaa acggtatccc aaaccttcca aacgtttgcg
     5941 ttcgctttca aagttttcga tgatgccgtt gtgtacgacc gcaatcatac cgccgctgat
     6001 gtgcgggtgg gcgttcggct cagtaacgcc gccgtgtgtc gcccaacgcg tatgtccgat
     6061 gccgatgccg ccgctgatgc ctttttcgcg tgccgcgtcc tccataagct gcacgcgtcc
     6121 gacgcggcgc acacgtttga ttttgccgtc ggtgttgacg gcaatgcctg atgagtcata
     6181 accccggtat tcgaggcgtt tgagaccgtc ggtcagaaaa tcgacgacgt tgtgatgggc
     6241 gcggatggcg ccgacgatac cgcacataac tgttccttag tatccggttg aaaaaaaaca
     6301 ggcgcggacg gcttccgtgc cgcaccttcc tcttcggatt ataaaccgcc tcccgcgccg
     6361 gaaaacagca aaatgccgtc tgaaggcttg ggcttgctca aaaaaaggag ggatttccct
     6421 gtttatccag gatgggcgtt cagacggcat tacctgctgc tggtttatag tttttgcaaa
     6481 tcaacattga caagctgaaa aaaaaaaaca atatactcgc tcggtcttaa tgttaacgga
     6541 gtatggaaat gaaacaaatg cttttagccg tcggcgtggt ggcggtgttg gcgggctgcg
     6601 gcaaggatgc cggcggttac gagggttatt ggcgcgaaaa gtcggacaaa aaagagggta
     6661 tgattgccgt caaaaaagaa aaaggcaatt acttccttaa taaaatccac gtggttacag
     6721 gcaaggaaga gtccttgctt ttgtctgaaa aagacggcgc gctttcgata aacacaggga
     6781 taggggaaat cccgatcaaa ctttccgacg acgggaaaga gctgtatgtc gaacgtaggc
     6841 agtatgtcaa aaccgatgcg gcgatgaagg acaaaatcat cgcccatcag aaaaagtgcg
     6901 gacaaacagc acaggcatac cgcgacgcgc gaaatgcgtt gccgtcaaac cagacgtatc
     6961 agcagcatct ggcggcgatc gagcaattga aacggcggtt tgaagccgag tttgacgaat
     7021 tggaaaaaga aatcaaatgc aacggcagaa gcccggcatt gttgctttag taggggacaa
     7081 ccgggaggat gccgccgtcc gaatcggatg tgcggtttct gtaccggtac gggcgggcag
     7141 gaatgtccgc cttttttgtt cggatgcgtt tgaatacccg tttgattccg accgtttgca
     7201 aggggtattt ccgttcgggc ggaaa
//
LOCUS       DAWIVB010000110         7204 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWIVB010000110 DAWIVB010000000
VERSION     DAWIVB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.110"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(64..148)
                     /locus_tag="WIX60_001481"
     tRNA            complement(64..148)
                     /locus_tag="WIX60_001481"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Leu,seq:caa)
     gene            425..1078
                     /locus_tag="WIX60_001482"
     CDS             425..1078
                     /locus_tag="WIX60_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ5305738.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1157..1810
                     /locus_tag="WIX60_001483"
     CDS             1157..1810
                     /locus_tag="WIX60_001483"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ5305739.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1914..2813
                     /locus_tag="WIX60_001484"
     CDS             1914..2813
                     /locus_tag="WIX60_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ5305740.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2810..4099
                     /gene="hisD"
                     /locus_tag="WIX60_001485"
     CDS             2810..4099
                     /gene="hisD"
                     /locus_tag="WIX60_001485"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ5305741.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4145..5242
                     /locus_tag="WIX60_001486"
     CDS             4145..5242
                     /locus_tag="WIX60_001486"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ5305742.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5239..6156
                     /gene="hisB"
                     /locus_tag="WIX60_001487"
     CDS             5239..6156
                     /gene="hisB"
                     /locus_tag="WIX60_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ5305743.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6251..7120
                     /locus_tag="WIX60_001488"
     CDS             6251..7120
                     /locus_tag="WIX60_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ5305744.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1879 a         2122 c         1723 g         1480 t
ORIGIN      
        1 ttagttttat ctatttcatt aaacagcaat agacaaaaaa aataaccgct ctaaaagcgg
       61 ttgtggtgcc cagggtcgga ctcgaaccga cacaccttgc ggcgggggat tttgagtccc
      121 ctgcgtctac caatttcgcc acctgggctg gtgaagaagt cgtcattata atggcttttg
      181 aaattctgta aacctttttt ttgaaattat tttatctgtt tttattttat ttttgatttt
      241 aaatagaatt tttattattt taatcttact gttctttccg ctccaaagat tctgtatgat
      301 tcggcaattc ctgccgtgca gacaacgtaa aaaaatacta cattaaatct gccaaacgcg
      361 ttaagatgga aatattcaaa ttccgtacga atcaggtttt gctatttatt cttgggagat
      421 tgtcatgttt tccgtaccgc gttccttttt gccgggcgtt ttcgtacttg ccgcgcttgc
      481 cgcctgcaaa cctcaagaca acagtgcggc gcaagtcgct tcttcaagtg catccgcgtc
      541 ggctgcggaa aatgcggcaa agccgcaaac gcgcggtacg gatatgcgta aggaagacat
      601 cggcggcgat ttcacgctga ccgacggcga aggcaagcct ttcagcctga gcgatttgaa
      661 aggcaaggtc gtgattctgt ctttcggctt tacgcactgt cccgatgtct gcccgacaga
      721 gcttttgacg tacagcgaca cgttgaagca gttgggcggg caggctaagg acgtgaaagt
      781 ggtgttcgtc agcatcgatc cggaacgcga cacgcctgaa atcatcggca agtatgccaa
      841 acagttcaat ccggacttta tcggtctgac ggcaacgggc gaccaaaacc tgccggtcat
      901 caagcagcaa taccgcgtgg tttctgccaa agtcaatcaa aaagacgaca gcgaaaacta
      961 tttggtcgac cactcttccg gtgcgtatct catcgacaaa aacggtgagg ttgccatttt
     1021 ctcgccttac ggaagcgagc cggaaacgat tgctgccgat gtaaggactc tgctctgata
     1081 aaaccgtatg ccgtctgcac cgtcggcgcc tattcagacg gcattattgt ttcaaccgac
     1141 aaaggacatc cacaccatgc aggataatgc tttgacaatc gccttatcca aggggcgcat
     1201 ttttgaggag acgctgccgc tgcttgccgc tgccggcatt gttccgactg aagagcctga
     1261 aaaatcgcgc aagctgatta tcgggacgaa ccatgaaaac atccgccttg tcattgtccg
     1321 cgcaaccgat gtgccgactt atgtccgcta cggcgcggcg gacttcggca ttgcgggcaa
     1381 agacgtgctg atcgaacacg gcggcacggg gctttaccgg cctttggatt tggagattgc
     1441 caagtgccgc atgatggttg ctgtgcgtaa agggtttgat tacgaagcag cttcgcaacc
     1501 cggatgccgt ctgaagattg ccacgaagta tcctgaaatc gcggcatctc attttgccgg
     1561 caagggtgtc catgtggaca ttatcaaact gtacggctcg atggaacttg cgccgctggt
     1621 cggcttgagc gatgcgattg tggacttggt ttcgacgggc aacaccttga aggcaaacgg
     1681 cttggaagca gtcgaacaca tcgtcgacat ttccagccgc ctggtggtca acaaggctgc
     1741 tttgaaaacg aaatacgcgc tgctggagcc gattattcag gcgttcggcg gcgcagtgaa
     1801 ggcgaagtaa gcatccattt gaataaagat gcgttttcag acgaccctat ccgttcccgc
     1861 cgacaggtcg tctgaaaata tcaccggcag taaactgtat aggagaagtt aaaatggttg
     1921 caaaaataaa aaaattctca gattcaaccc tttccgtttt gaataacggc gagcgtcggt
     1981 tttatgtcta ttgtctgacc gacctgaaaa aagacaaaat cctctacatc ggcaaaggct
     2041 gcggtaatcg tatcttcgag catgaatggg ttgctagtcg ttcacaagac ccagtctccg
     2101 gcgagattat cgatcggaaa ctcaaagcca tctccaaatg caagaaactc ggtcgctata
     2161 tcatcagcta tcatctgact gaagtcgaag cactcgccgc cgaatctgcc ttaattcatt
     2221 ttgttaaatc tgtcttgggt aaaaaactca aaaataaaat tgccgggcat ggtccgggtg
     2281 gtattagcgt agaagaacta gatcgccgct ttggattctc ttctctccca cttaacgaga
     2341 ttaaccccga cgggctgatt ctcgccatca aaatccacaa tgctttcgat ttagatactg
     2401 acgaagaatt agactacctt ttcgacaacc aagacgatgc caacctcaaa tcgcgtacgt
     2461 tgggcaactg ggttatcggt aaagatgttg cttcaaaagt gaaatacgtt atcggcgttc
     2521 acaccggtct gcaaaacgct gttgtcagtg catacgaagt ggacggtttt gaaacaatgg
     2581 ttgaggaaac caaaaacggt agaaaacaat cccgttaccg tttccgcact acctctcgta
     2641 gcgaagaggt attagccaaa ctcggtctgc aacaaaaatg cctgcccgaa ttgaagtttg
     2701 gtagcggggg agaaaaagcg tatatcagac ccaaaacaga gacagaaact gaacaagaga
     2761 atattcagac gacccccaat ccaaaaataa aaaaggaaaa aaccaaatca tgaaaaaact
     2821 caacacccaa tcgcccgatt tccaagccgg actcaaagcc ctgctggctt ttgaaaccgc
     2881 gcaaaacccc gaaaccgaac gcatcgtcgc cgacatttgc gccgacgtgc aaaagcgcgg
     2941 cgatgcggct ttgattgaat acaccaacaa attcgatcag acaaacgcta aaagcatcga
     3001 tgatttaata ctcacgcaag ccgatttgaa cgcggcgttc gagcgcattc cgaacgacgt
     3061 tcagacggca ttgcagaccg ccgcccgccg tgtcgaaagc taccaccaac gccaaaaaat
     3121 ggaatcgtgg agctacaccg atgaagacgg cacgctgttg ggacaacaga ttaccccgct
     3181 tgaccgcgtc ggcatttacg tccccggcgg caaggcggca tatccgagtt ccgtcatcat
     3241 gaacgccatg cccgcccacg tcgcaggcgt gaaagaaatc atcatggtcg tgccgacacc
     3301 gaaaggcgaa cgcaacgaca tcgtgcttgc cgccgcatac gtcgccggcg taaccaaagt
     3361 cttcaccgtc ggcggcgcgc aggcggttgc cgccctcgcc tacggcacgg aaaccatccc
     3421 ccaagtcgat aaaatcaccg gtccgggcaa cgccttcgtc gccgccgcca aacgccgcgt
     3481 gttcggcgtg gtcggcatcg acatggtggc ggggccgtct gaaatcctgg tcatcgccga
     3541 cggcacgaca cctgccgatt gggtggcgat ggatttgttc agccaggccg aacacgacga
     3601 aattgcccaa gccatcctca tcggcacgtc gcaagcgtat ctcgacgaag tagaagccgc
     3661 tatggaccgc ctgatcgaaa ctatgccgcg ccgcgacatc atcgaagcct cgctcggcaa
     3721 caggggcgcg atgatactcg ccaaagactt ggacgaagcc tgcgaaatcg ccaactacat
     3781 ttcccccgaa cacttggaac tgtcagtcga aaatccgcag gaatgggcga aaaaaatccg
     3841 ccacgccggt gcgattttca tgggacgcta caccggcgaa agcctcggcg actactgcgc
     3901 cggtccaaac catgtattgc ccaccagccg aaccgcccgc ttttcctcgc ctttggggac
     3961 atatgatttc caaaaacgct ccagcctgat tcaggtttcg gaacagggcg cgcaaaaatt
     4021 aggcgaaacc gccagcgtgc tggcacacgg cgaaagcctg accgcccacg cccgcgcggc
     4081 agagttccgt atgaaataat gccgaaacgg cgtacaggca tattccaacc attaaggaaa
     4141 cacgatgaaa tccgtccgct ccttcatccg cgacgacata caagctatgt cggcatatca
     4201 gattgccgac gttccgcccg gctttgccaa actcgattcg atggaaagtc ccgtccaccc
     4261 ttttgccgga catgaaacgc tgttgcagga atggcaggca cggcttgccg ccgcgcccat
     4321 ccatctttac cccaatccct ccggcagcgg tttacaggaa gcattacgtt cggcgttcga
     4381 cattcccgac tgcgccgaca tcgcgctggg caacggctcg gacgagctga tacagttcat
     4441 cacgatgctg accgccaaac cgggcgcggc aatgttggca gccgaaccca gtttcgtcat
     4501 gtaccgccac aacgccgcgc tgtacggcat ggattatgtc ggcgttccac tgaacggaga
     4561 tttcaccctc aacctgcccg ccgtcctcga agccgtcagg aagcaccgcc ctgccctgac
     4621 ctttatcgcc taccccaaca accccaccgg cgtatgtttc acgcgtgccg aaatcgaagc
     4681 cgtcatcgaa gcttcagacg gcatcgtcgt cgtcgacgaa gcctacggcg cattcaacgg
     4741 cgacagcttc ctgccgcagg cgggcagcat tcccaacctc atcgtcatgc gtaccgtcag
     4801 caaaatcggt tttgccggac tgcgtatcgg ctatgcggca ggctgtcccg aagtcatcgg
     4861 cgaactgcaa aaaatcctgc cgccctacaa tatgaaccaa ctgagcctga ccaccgccaa
     4921 actcgccctg caacactacg gcatcatctc tgccaacatc gacagcctga aaaacgaacg
     4981 cgaacggatg ttcgccgaat tgggcaaaat atgccgtctg aacacctttc caagtcaggc
     5041 aaacttcatt accatacgcg tacccgatgc cgatttgttg tttgacacgc tcaaacaaaa
     5101 ccgcatcttg gttaaaaaac tgcatggcgc gcacccgctt ttggaacact gcctgcgcat
     5161 taccgtaggc agtcccgcac aaaacgatgc cgttctcaac atcattcgcc aactttactg
     5221 ccaaccaacg gatttcctat gaatttgact aaaacacaac gccaactgca caactttctg
     5281 accctcgccc aagaagcagg ttcactgtcc aagctcgcca aactctgcgg ctaccgtacc
     5341 cctgtcgcac tctacaaact caaacaacgc cttgaaaagc aggcagaaga cccagatgca
     5401 cgcggcatcc gtcccagcct gatggcaaaa ctcgaaaaac acaccggcaa acccaaaggc
     5461 tggctcgaca gaaaacaccg cgaacgcact gtccccgaaa ccgccgcaga aagcaccgga
     5521 actgccgaaa cccaaattgc cgaaaccgca tctgctgccg gctgccgcag cgttaccgtc
     5581 aaccgcaata cctgcgaaac ccaaatcacc gtctccatca acctcgacgg cagcggcaaa
     5641 agcaggctgg ataccggcgt acccttcctc gaacacatga tcgatcaaat cgcccgccac
     5701 ggcatgattg acatcgacat cagctgcaaa ggcgacctgc acatcgacga ccaccacacc
     5761 gccgaagaca tcggcatcac actcggacaa gcaatccggc aggcactcgg cgacaaaaaa
     5821 ggcatccgcc gttacggaca ttcctacgtc ccgctcgacg aagccctcag ccgcgtcgtc
     5881 atcgaccttt ccggccgccc cggactcgtg tacaacatcg aatttacccg cgcactaatc
     5941 ggacgtttcg atgtcgattt gtttgaagaa tttttccacg gcatcgtcaa ccacagtatg
     6001 atgaccctgc acatcgacaa cctcagcggc aaaaacgccc accatcaggc ggaaaccgta
     6061 ttcaaagcct tcgggcgcgc cctgcgtatg gcagtcgaac acgacccgcg catggcagga
     6121 cagaccccct cgaccaaagg cacgctgacc gcataaaaaa ccataccgtc tgaaacaccc
     6181 gcaggctttt cagacggtat cggaacagat aagattacac tacactacaa acagaaaagg
     6241 agtaaacatc atgtccgcaa acgaatacgc acaaatcggc tggataggct tagggcaaat
     6301 gggtctgcct atggtaacgc ggctcttgga cggcggcatc gaagtcggcg tatacaaccg
     6361 ctctcccgac aaaactgccc ccatctccgc caaaggcgca aaagtttacg gcaacaccgc
     6421 cgaactcgtc cgcgactatc ccgtcatttt cctgatggtt tccgactatg ccgccgtgtg
     6481 cgacatcctg aacggagtcc gcgacggatt ggccggcaaa atcatcgtca acatgagcac
     6541 catctccccg accgaaaacc tcgccgtcaa agcacttgtc gaagccgcag gcggacagtt
     6601 tgccgaagca cccgtttccg gatcggtcgg gcccgccacc aacggcacgc tgctgattct
     6661 gttcggcggc agcgaagccg ttttaaaccc gctgcaaaaa atattttccc tcgtcggcaa
     6721 aaaaaccttc catttcggcg atgtcggcaa aggttcgggc gcgaaactcg tcttgaactc
     6781 gctcttgggc attttcggcg aagcgtacag cgaagcgatg ctgatggcgc ggcagttcgg
     6841 catcgatacc gacaccatcg tcgaagccat cggcggctcg gcaatggact cgcccatgtt
     6901 ccaaaccaaa aaatccctgt gggcaaaccg cgaattcccg cccgccttcg ccctcaaaca
     6961 cgcctccaaa gacctcaacc tcgccgtcaa agagcttgaa caggcaggca acaccctgcc
     7021 cgccgtcgaa accgttgctg ccagctaccg caaagcagtc gaagccggct acggcgaaca
     7081 ggacgtttcc ggcgtttacc tgaaactggc agaacactga ttgccttttc caaacacaat
     7141 gccgtctgaa catatttcag acggcatttt tatcacccca cgcttaaaat cagtcccgat
     7201 tatg
//
LOCUS       DAWIVB010000111         7164 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWIVB010000111 DAWIVB010000000
VERSION     DAWIVB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.111"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            267..488
                     /locus_tag="WIX60_001489"
     CDS             267..488
                     /locus_tag="WIX60_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305745.1"
                     /translation="MAVWRDYITLESHDLAEGYTYFFNENTDDCYVLKEDFINNERYE
                     KTELYSQKDKVILFPKFGEYDLVLNPDII"
     gene            675..947
                     /locus_tag="WIX60_001490"
     CDS             675..947
                     /locus_tag="WIX60_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305746.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            1043..1909
                     /locus_tag="WIX60_001491"
                     /pseudo
     CDS             1043..1909
                     /locus_tag="WIX60_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            1928..2191
                     /locus_tag="WIX60_001492"
     CDS             1928..2191
                     /locus_tag="WIX60_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305747.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            2298..4220
                     /locus_tag="WIX60_001493"
     CDS             2298..4220
                     /locus_tag="WIX60_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ5305748.1"
                     /translation="MQLLAAEGIHQHQLNVQKSTRFIGIKVGKSNYSKNELNETKLPV
                     RVIAQTAKTRSGWDTVLEGTEFKTTLSGADIQAGVGEKARADAKIILKGIVNRIQTEE
                     KLESNSTVWQKQAGSGSTVETLKLPSFEGPALPKLTAPGGYIADIPKGNLKTEIEKLA
                     KQPEYAYLKQLQTVKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTSGAGTG
                     AVLGLNGAAAAATDAAFASLASQASVSFINNKGNIGNTLKELGRSSTVKNLMVAVATA
                     GVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAALV
                     NTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEILGETLL
                     DGRDPGSLNVKDRAKIIAKAKLAAGAVAALSKGDVSTAANAAAVAVENNSLNDIQDRL
                     LSGNYALCMSAGGAESFCESYRPLGLPHFVSVSGEMKLPNKFGNRMVNGKLIINTRNG
                     NVYFSVGKIWSTVKSTKSNISGVSVGWVLNVSPNDYLKEASMNDFRNSNQNKAYAEMI
                     SQTLVGESVGGSLCLTRACFSVSSTISKSKSPFKDSKIIGEIGLGSGVAAGVEKTIYI
                     GNIKDIDKFISANIKK"
     gene            4229..4651
                     /locus_tag="WIX60_001494"
     CDS             4229..4651
                     /locus_tag="WIX60_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305749.1"
                     /translation="MKYMISFLKKTFELMSWVLVILIIGTFYDYYQIRQYAELEKKSI
                     SNILLYAQKEKFRLESKDKYMRGGYTKYKFIFSEYSNTTFLNFINDLKKDNYLPLDGY
                     GHGFLCRKGESISINIYPEINKFILVWGYPENLCADSN"
     gene            4688..5089
                     /locus_tag="WIX60_001495"
     CDS             4688..5089
                     /locus_tag="WIX60_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ5305750.1"
                     /translation="MRKLNNHDVHKRYQDRLEEDVEFTINYELPLSCLWSTIKDFSSD
                     FEEKTEAFFILFKELLRRGHLKLQRDGQIIGHTPEEWEQIFREVWPEYEIEPNPLPGY
                     APFDIGMWLTVEAPAYAVWIDPEDGSEYWAG"
     gene            5212..5832
                     /locus_tag="WIX60_001496"
     CDS             5212..5832
                     /locus_tag="WIX60_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305751.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKTISEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GANNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIKQVITNLPD
                     NHPTKNILREAVRSGKIKTAVTGVDRQTGKAVILPVKVPSKTNIRR"
     gene            5835..6773
                     /locus_tag="WIX60_001497"
     CDS             5835..6773
                     /locus_tag="WIX60_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ5305752.1"
                     /translation="MGHNMMTTQKWYEHITDVIIDTTQRFNREKSADSIEYINERKGS
                     PIGAICVVSIDVYAACSHAYLFEHNLKKFKQYAYAYSKLEILASMGWSDPTPFFFPCD
                     MLNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGK
                     QLDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARM
                     AAKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPADEYQDIFD
                     FMKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT         2284 a         1339 c         1655 g         1886 t
ORIGIN      
        1 tgaagaaaga tattttttat tgtgagcagt ggtcttatgg ttataagaaa cttcataagc
       61 ctttttctga gaaacaagct gaggaaaaac atcttaaagg ggagttatat actgccgtaa
      121 taggttcggc gacacaacct gaatatgtaa ttaccttgcg agaggaagta ggtttttttt
      181 cggtacattt tttcgataaa tttggaaggg attatttaac ccatcaattt caaaaatatt
      241 ccaattcgaa ttattatttt ctttctatgg ctgtatggag agattatata actttggaat
      301 ctcatgactt agcagaagga tatacttatt tcttcaatga aaatacggat gattgctatg
      361 ttttgaaaga ggattttatt aataatgagc gatatgaaaa aacagaatta tattcccaaa
      421 aagataaggt aattctattt ccaaagtttg gcgaatatga tttggtgtta aatccggaca
      481 ttatttaatt gagttttaag gccgtctgaa aaaatttcag acggctttta ttattgggtt
      541 tggaatctga ggataaagct gataaaaacc aggaaattat caggttgcta tatacgtatt
      601 gttgtacaga ctaaaggcag caatcaaatc actactgctt acccacaaaa ataaatcgat
      661 tatatggagt aatcatgaat aagagaatga aaatgtgtcc tgcttgtcaa caaggctatc
      721 tctaccattc gaaacctaaa tatcttcatg atgaaattat tctgtgtgat gaatgcgatg
      781 cagtatggct caaaggtatg aatatatttt atggagaata tgaaaaagat ttttattctt
      841 atgttccttt catggaatcc caaggtataa cgagtgaatg tatttgggaa ggagatttgt
      901 ttgatcatcc atattatgaa gatgaaaact caaatgatat ggattgatgg aaattttaag
      961 cctgcgtagg tacgattagc catcaaacgg cgtaatcata cgcaagatta tcaacagaga
     1021 gggctggcag cgatatacca cccacaagat tgcccatgcc atagcgggct gtgcggcagc
     1081 ggcggcgaat aagggcaagt gtcaggacgg cgcgattggt gcggtcgtgg gggagattgt
     1141 cggggaggct ttggttaaga ataccgattt cagcggtatg actgcttctg aaattgaaaa
     1201 agctaaagcg aatattactg cgtatgcaaa attggtagcc ggagcgactg taggtgttac
     1261 aggaggcaat gttgatgtgg cggcaaatgc ttccgaaaca gctgttaaaa ataatgcatt
     1321 agatattatt tgggatattg gcaacctcgt atgggacggc ggtaaatgga tttacgccaa
     1381 atctattggc gataagcaga tggctcgaga agcggcgatt gattttggtg tggatgccgc
     1441 cgcagctgcc gttccctttg ttccggcagg tgcgactaaa atcagccgag gcggggctta
     1501 tgttctgaag gcgggagacg aagcagttga tacggctaaa gccatacagg aaattcagaa
     1561 gcagaccgga atcaagctta cttatgataa ggttaataag gtttggacaa caccggcggg
     1621 gttagattat gggttagatg ctaagcatgg taataggatt aaacatgttt tagcccatac
     1681 aattccaaat ccaaacaaac ctgttcattc tgtttttaat gtgtcccgta aagaagtttt
     1741 gcctttggtt gatgaagctt ggagaatgaa aggaaatcct ttgccaaatg attcatccgt
     1801 atatcttgta gatatgaaga aacctattgg aacaaaagga gaaacaaaag tgcggattgt
     1861 tgtgcaaaaa ggaacaaata aaatcatttc tgcatatcct cagaaataat taagaaagga
     1921 atctcttatg gataaagaaa ttaaaatttg cccaagatgt gagcaaggct acctttatca
     1981 tgcaaagcct aaatatttct ctggggaggt cattttatgc gatgaatgtt atgctatgtg
     2041 gcttggggat atgaaaattt tttacggaca atatggaaaa gatttttatg attatcatga
     2101 gtttatgaaa gataaaggca tagaagaaat aaatatgtgg gaaggagagc tttttgatca
     2161 cccatattat gaggatgaaa aatttaaata attgattttc tgttccccga atttgggaaa
     2221 tacgatgata ttttaaaccc aaatattatt taaagtagca ataggccgtc tgaatatccg
     2281 tttttcagac ggcctcaatg caactgctgg cagccgaagg cattcaccaa caccaattga
     2341 atgttcagaa aagtacccgt ttcatcggca tcaaagtggg taaaagcaat tacagcaaaa
     2401 acgagctgaa cgaaaccaaa ctgcccgtac gcgttatcgc ccaaacagcc aaaacccgtt
     2461 ccggctggga taccgtactc gaaggcaccg aattcaaaac caccctttcc ggagccgaca
     2521 tacaggcagg ggtgggtgaa aaagcccgag ccgatgcgaa aattatccta aaaggcatcg
     2581 ttaaccgcat ccaaaccgaa gaaaagctgg aatccaactc gaccgtatgg caaaagcagg
     2641 ccggaagcgg cagcacggtt gaaacgctga agctaccgag ctttgaaggg ccggcactgc
     2701 ctaagctgac cgctcccggc ggctatatcg ccgacatccc caaaggcaac ctcaaaaccg
     2761 aaatcgaaaa gctggccaaa cagcccgaat atgcctatct gaaacagctt cagacggtca
     2821 aggacgtgaa ctggaaccaa gtacagctcg cttacgacaa atgggactat aaacaggaag
     2881 gcctaaccgg agccggagcc gcaattatcg cactggccgt taccgtggtc acctcaggcg
     2941 caggaaccgg agccgtattg ggattaaacg gtgcggccgc cgccgcaacc gatgcagcat
     3001 ttgcctcttt ggccagccag gcttccgtat cgttcatcaa caacaaaggc aatatcggta
     3061 acaccctgaa agagctgggc agaagcagca cggtgaaaaa tctgatggtt gccgtcgcta
     3121 ccgcaggcgt agccgacaaa atcggtgctt cggcactgaa caatgtcagc gataagcagt
     3181 ggatcaacaa cctgaccgtc aacctggcca atgcgggcag tgccgcactg attaataccg
     3241 ctgtcaacgg cggcagcctg aaagacaatc tggaagcgaa tatccttgcg gctttggtga
     3301 atactgcgca tggagaggca gcaagtaaaa tcaaacagtt ggatcagcac tacattgccc
     3361 ataagattgc ccatgccata gcgggctgtg cggcagcggc ggcgaataag ggcaagtgtc
     3421 aagatggtgc gatcggtgcg gcggtcggtg aaatccttgg cgaaacccta ctggacggca
     3481 gagaccctgg cagcctgaat gtgaaggaca gggcaaaaat cattgctaag gcgaagctgg
     3541 cagcaggggc ggttgcggcg ttgagtaagg gggatgtgag tacggcggcg aatgcggctg
     3601 ctgtggcggt agagaataat tctttaaatg atatacagga tcgtttgttg agtggaaatt
     3661 atgctttatg tatgagtgca ggaggagcag aaagcttttg tgagtcttat cgaccactgg
     3721 gcttgccaca ctttgtaagt gtttcaggag aaatgaaatt acctaataaa ttcgggaatc
     3781 gtatggttaa tggaaaatta attattaaca ctagaaatgg caatgtatat ttctctgtag
     3841 gtaaaatatg gagtactgta aaatcaacaa aatcaaatat aagtggggta tctgtcggtt
     3901 gggttttaaa tgtttcccct aatgattatt taaaagaagc atctatgaat gatttcagaa
     3961 atagtaatca aaataaagcc tatgcagaaa tgatttccca gactttggta ggtgagagtg
     4021 ttggtggtag tctttgtctg acaagagcct gcttttcggt aagttcaaca atatctaaat
     4081 ctaaatctcc ttttaaagat tcaaaaatta ttggggaaat cggtttggga agtggtgttg
     4141 ctgcaggagt agaaaaaaca atatacatag gtaacataaa agatattgat aaatttatta
     4201 gtgcaaacat aaaaaaatag gagttagtat gaaatatatg attagttttc taaaaaaaac
     4261 atttgaatta atgagttggg tgttagtcat actaataatt gggacatttt atgactatta
     4321 tcaaataagg caatatgctg aattagaaaa gaaatctata tcaaatatct tgctatatgc
     4381 ccaaaaagaa aaatttcgct tagagagtaa agataaatac atgcgaggag gatatacaaa
     4441 atataaattt attttttcag aatatagtaa tactactttt ttaaatttca taaatgacct
     4501 gaaaaaagat aattatttac cacttgacgg ctatggacat ggttttctat gtagaaaagg
     4561 agagtctata tcaatcaata tataccctga aattaataaa tttattttag tatggggata
     4621 ccctgaaaat ctttgcgctg attctaatta agtaagcaag aataggttat agggaaataa
     4681 aattcaaatg agaaaattga ataatcacga tgttcataaa cggtatcaag atcgtttaga
     4741 agaggatgta gagttcacta tcaactatga gcttcctttg agttgtttgt ggtcaaccat
     4801 caaagacttt tccagcgatt ttgaggaaaa aactgaagcg ttctttattc ttttcaaaga
     4861 gctgctgcgc agaggtcatc tgaaactgca acgcgacggg caaattatcg ggcatacgcc
     4921 cgaagaatgg gaacaaatat ttagggaagt atggcctgaa tatgaaatcg aacccaatcc
     4981 acttcccggc tatgccccat ttgatattgg aatgtggctt acggtcgagg ctcctgccta
     5041 cgccgtatgg atagatcccg aagacggtag cgaatactgg gcgggataaa ataccaatgt
     5101 ttggaataaa tcccgtctga aaaacagctt tttcagacag gatttattcc aattatcggt
     5161 gatatacaga gttttgtaca agcacagacc gctgccgatc acctgtttgc tttgctgggt
     5221 gtggttccgg gtatcggtga atcgatacag gcctataaag tagcgaaagc ggcaaaaaat
     5281 ttacaaggca tgaaaaaagc cttggacaag gcagcaaccg ttgccactgc acagggctat
     5341 gtcagtaaaa ccaaaatcaa aatcggtcaa actgaattaa gggttactgc agcaactgac
     5401 aaacaattgc tgaaaaccat tagcgaagga agggacacga caggtaaaat gaccgagcag
     5461 ttatttgact ctttagctaa acaaaatggc ttcagagtgc tttcgggcgg caaatacggc
     5521 gcaaataacg gttttgatca tgtatggcag gctgccgatg gtagtgtcgt tttgattgta
     5581 gaaagtaagc agattaggaa cggtacggta cagctgaatc cgaatggtgc gggtggatat
     5641 acgcaaatga gtgaggattg gattaaacaa gttattacta acttaccaga taatcatccc
     5701 actaaaaaca tccttcgaga agcagtacgt tcgggtaaaa tcaaaactgc ggttactgga
     5761 gtagatcgtc aaacaggtaa agcagttatt cttcctgtta aagttccttc taaaaccaat
     5821 ataaggagat aacaatgggg cacaatatga tgaccaccca aaaatggtat gaacatatta
     5881 cagatgtgat tatagacacc actcaaagat ttaatagaga gaaaagcgct gatagcatag
     5941 agtatattaa tgaaagaaag ggatctccaa ttggagcgat ctgtgtcgtt tctatagatg
     6001 tttatgcggc atgctcacat gcttatctgt ttgaacacaa ccttaaaaag ttcaaacagt
     6061 atgcttatgc ttacagtaaa cttgagatac tggcaagtat gggatggtct gaccccacgc
     6121 ctttcttctt tccctgtgac atgctcaaca ttcaaaatcc gatgtttctg atgctgatga
     6181 gcgacagccc gcagctgcgc gagtttctgg tgcgcaatat cgacaacatc gccaacgata
     6241 cagaagcctt cgtaaaccga tacgacctca accgtcatat gatttacaat actctgctga
     6301 tggtagaggg taagcagctt gatcggttga aacaacgtag cgagaaagtc ttggcgcatc
     6361 ccacccctag caaatggctg caaaagcggt tgtacgatta ccgcttcttc ctcgctttcg
     6421 ccgaacagga tgccgaggcg atgaaggccg ccttagagcc gcttttcgat aaaaaaaccg
     6481 cgcgtatggc tgccaaagaa acattgtcct atttcgattt ctacctgcag ccgcaaatcg
     6541 ttacctacgc caaaatcgca tccatgcacg gtttcgattt gggcatagat caagaaatcg
     6601 caccgaggga tttgattgtt tacgatccgc tgccggcaga cgaatatcaa gacatcttcg
     6661 attttatgaa acagtatgac ttgtcttacc cgtatgaata tctgcaggat tggatagatt
     6721 actatacgtt caaaaccgat aagctggtat ttggtaacgc gaagcgagag tgagccgtaa
     6781 aactctgagc tcctgtttta tagattacaa ctttaggccg tcttaaagct gaaagatttt
     6841 cgaaagctat aaattgaagc ccttccatag tacatagatc tgtgttgtgg cggggcttta
     6901 ccacgctgat tgccggagaa gaactcaacc tgctggcaaa acaaggcatg agatctttgc
     6961 aataacatga gttgagacct ttgcaaaaaa gcccttcccc gacatccgaa acccaaacac
     7021 aggatttcgg ctgttttcgt atcaaatacc tcctaatttt acccaaatat ccccttaatc
     7081 ctccccggat accccataat agagcatccg ctccgccttt taggcggtag cgagcgtact
     7141 tagcctgttg gcggctttca acag
//
LOCUS       DAWIVB010000112         7113 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWIVB010000112 DAWIVB010000000
VERSION     DAWIVB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.112"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(150..590)
                     /locus_tag="WIX60_001498"
     CDS             complement(150..590)
                     /locus_tag="WIX60_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305753.1"
                     /translation="MIKETLMRPIFLSFVLFPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(831..1010)
                     /locus_tag="WIX60_001499"
     CDS             complement(831..1010)
                     /locus_tag="WIX60_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ5305754.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            complement(1016..1603)
                     /locus_tag="WIX60_001500"
     CDS             complement(1016..1603)
                     /locus_tag="WIX60_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ5305755.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTQEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(1625..2371)
                     /locus_tag="WIX60_001501"
     CDS             complement(1625..2371)
                     /locus_tag="WIX60_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ5305756.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIYS
                     FIDRCYTETLQYFDREEWGRLKEGFVRDACAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGFSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEDNSPGQNHLSA"
     gene            complement(2361..3203)
                     /locus_tag="WIX60_001502"
     CDS             complement(2361..3203)
                     /locus_tag="WIX60_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ5305757.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAERVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFSELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            complement(3319..3627)
                     /locus_tag="WIX60_001503"
     CDS             complement(3319..3627)
                     /locus_tag="WIX60_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305758.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            complement(3700..4149)
                     /locus_tag="WIX60_001504"
     CDS             complement(3700..4149)
                     /locus_tag="WIX60_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ5305759.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPRCRLKQD"
     gene            4494..5387
                     /locus_tag="WIX60_001505"
     CDS             4494..5387
                     /locus_tag="WIX60_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ5305760.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAVRKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            5490..6593
                     /gene="prfB"
                     /locus_tag="WIX60_001506"
     CDS             5490..6593
                     /gene="prfB"
                     /locus_tag="WIX60_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ5305761.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFRIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            6672..7031
                     /gene="rnpB"
                     /locus_tag="WIX60_001507"
     ncRNA           6672..7031
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WIX60_001507"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1798 a         1624 c         1885 g         1806 t
ORIGIN      
        1 gatggaagcc ggttttaaaa aataatgtgg ataactttgt ggataatttt cagggaatgg
       61 caaaatgccg tctgaaatgc tgatatttca gtatttcaga cggcattttt atgataaggt
      121 ttgtgggtaa gtttgtgtat aagttgcaat cagagttttt ttccgcatac ggtttcatat
      181 tgtttttcgg ttaatgtccc ggacaggatg ctcatcgggc ggagggaaga ggctgtgtag
      241 ttttgtgtgg aaatttccgt gttttttgta tcaaacaact gtagcgaact taagcggtat
      301 gttttgttgt tgcagtggat ttcccactcg gcaatggcag tcttgtatgc gggggtgttg
      361 gcaaaacgtt cttgttttag attggtaaca acttttttat cttggaaaat catcagattt
      421 ccgttttttc tcacgctgtc tttattgata tatgtatgaa tattgccgtt tgagattgtg
      481 ccgatatttt cccatcgggc agacttgtcc ggtgtgctgc aggcggttat caaaataggg
      541 aataaaacga aagataggaa gattgggcgc attagggttt ccttaatcat tgaaatctgc
      601 tcattcagaa agaacgggtt gggcgggagg ggtagcgccg tctgtgaaat aaggcaaata
      661 aagcaaatat ggcaaattga tgcagccttc gtattttgcg tatttataca ttccgtatgg
      721 ggaaatatgc tgcttttgaa gtgtgtatta tggatgaact gcagataaca gtaaatcggt
      781 ttgatttgtt tccgtcagct tatgccgtct gaagcctttt cagacggcat ttattttgaa
      841 gttctggttg tttttgccag tgatcttttg atggtttgaa gttgtctttt atattcacgg
      901 caatacggac agaacagcag gtgtgtgtat atggaaatct tctcgcccgg ggtggtttcc
      961 cggtcctgat gtttggaaag aagcagggcg atatcgcggc attttttcat aacgtttact
     1021 tcgggttttc ttggttgaac catttgattt gcaggcattg gcgcaatgat tctcgggcgc
     1081 ggtgcataat ggtgtggtag ttggacgtgc tgataccgca catttgttgt atttcgtcgg
     1141 atgaaaaacc gagtatttcc ttcagggtaa atacccgtgc ggtgttttca ggcaggttgt
     1201 ataggcagct ttgcagaatt ttttggaatt cgttgttgtt taatgatttt tccggagtgt
     1261 tccaatgttg cggctgccct tcctgcgtcc aatgcccgtt ttgggaaaaa tggctttcaa
     1321 atgcttcatc cagtagctcg tcatccagtg tggtaaagac tttcctctgc cttccgattt
     1381 gacgtaatgc gtcaataatt ttgtttttca atatggcaaa aagccagctg ttgacaagtg
     1441 ccctgccttg aaaactgtcg cctgcgctgt atgcggacag caatgtttcc tgcactaaat
     1501 cttcagccaa atcagggtgg tcgggcaact gaagccgcgc aaaatgcaga agcagtttac
     1561 gcgactctat taattcggca tcggtcaggt cgggtagcgg catagtatcg ggaacaaggt
     1621 aattttatgc ggatagatgg ttttggcccg gggaattgtc ttccatattt tcggatatgg
     1681 cggacaagga ggggatgata atcctttgtt cagtccagcc tgaccatttc ccaagcaaaa
     1741 tatttttcca attatcgtcc tcaggcataa attcgacaag ggtttgggcg agggtgtcaa
     1801 acgaaagcgc ggagaacccc attatttcta gcagcatcat atcgaagccg tccaatgttt
     1861 ggtacatcac atcatcttcg gcgtttcgcc atattaacaa ggctgtttcc gcttcatgca
     1921 aatcatcggt aacatcatat cgatattgcc ggataaaggc cgcaggggaa ggtgtgtatt
     1981 tgctgtcatt tgaataatga atgtcgggaa tatccggaat ttgagcaact tctgccagca
     2041 attgggtata ttcaaaatcc atcagtgcca aaatgccgtc taaaagcggc aggctttggc
     2101 aatattggag gaactcgccg gggatttctt gaaaataggg cgtttgggcg cacgcgtcgc
     2161 ggacgaaacc ttctttcaga cggccccatt cttcgcggtc aaagtattgc agcgtttcgg
     2221 tataacaacg gtcgataaag ctgtagatat tgttgcgtat caggcggata tagacgttca
     2281 gtcggtcttg cggcagaccg tctgcggctt cgcccccgcg tatggcttgg gcgaaacggt
     2341 gctggtattg ggcggaggtt tcaggctgca cggcggcatt cctttccggc acgcgtttga
     2401 taatcggcga ttttggcgac ttcggcttcg agttcggaaa aaggcgggaa attaaaatcg
     2461 cgttccaaca gggtgggcgg aatcgtcggc agcttggcat aggcaagttc gagcaagtcc
     2521 caaacagtcg gcaaaactgc cgcgccatgt gtatcaatca acaattccgg cgtttccacg
     2581 tcatgtccgg caatatggat atagcacacg cgctctgcat ccacattttc caaaaaagcc
     2641 tccggcgaca gcagaccgtg attgacggcg ttgacgtaga tattgttcac atccagatga
     2701 atgccgcaat cggcctcacg tgcgacggcg ttgaggaact cgacctcgtt catctcggca
     2761 agcggggaat gcagatagta ggacgtgttt tccacggcga tgcggcagcc caaacggtct
     2821 tgcacttcgc ggatacgccg cgccgtatga tgcaccattt cctcggtaaa gggcagcggc
     2881 aacaaatcgt aaagatgacc gccgtcgtgg cagtagctca aatggtcgga gaaaaacgtg
     2941 caatcgtaac ggcgcatcat ttctttgatg ccgtctatca aatcagtatc cagcggtgct
     3001 tgcccgccca gcgacataga caatccgtgc aacgccagcg gcagccgttc cgccacacgg
     3061 tcaaactgtt tgcgcgccca gccgcccatt ttcagccagt tttccggtgc ggcttcgata
     3121 aagcatatcg ggctgttttc ggaaagcgag agaaagtctt ccgccaagtc gcggcggtag
     3181 cccaagcctg cgtgttgaat catattttta cccggttcgg atggcacaaa agaatgatgt
     3241 gcttgttaaa aaatctttgt ttgccgttaa aaaagccgca ttttactgaa aaacgcggct
     3301 tggtttgaag gggtgggatt atttagaacc gcatttgcct tcgccgcatt tgccttcggc
     3361 agatttggcc ttggctttag atgctttggt gtgtttgtgg gtttttttta cggtcgcacc
     3421 gcatttgccc tcgccgcatt tgccttcgcc tgctttagaa ccagccgcgc cgcacgaacc
     3481 ttcggcagat ttggacgcgc cgcaagagcc atgggcggat ttatgaacgc ctgttgcgtt
     3541 gcttgccggt ttgttggcag caactgcacc ggcggccaaa gacagggata aagcaccggc
     3601 gagagcggca gcaatgtttt tgttcatgtc atttcctttt caaaatatca agttaacgag
     3661 ataaggttgg aatgtttaaa gtcagattcc acgactgaat caatcttgtt tcagacggca
     3721 tcgggggcaa aaccgttttt tcagcagcgt atccagcgac catccgcccg caccctggaa
     3781 aagcagcggg attaatacca cgatataaat taaagccatt ttataaccgt tgtcgcagac
     3841 attgtaaccc gaaccggcgt gaaccgcagc ccaagcgacg gcggtaacga ccatcagccc
     3901 caatgccgac agacgcgttg ccaaacccaa aagcaacaat acgggcagca aaagctccgc
     3961 atacatggcg agattccagt ttaacgcgtc cggcagcaag ttgaacggga atggaaactg
     4021 atcgttgatt tcggaaaacc aattctcccc gttccatttt tgcaaacccg attccaaaaa
     4081 ttcatatgcg gcaaacagac gcaaaaccag catagcggat gccgtctgcc aagttgttcc
     4141 gattttcata agtgttcctg ttttggttct gttgataccc cattagacgg attttgtcgg
     4201 aaaaactgac acccggcagc caatgattta acaagttaat gaatataaaa agaaagtatt
     4261 ttgatggaaa gaaagggaaa aaggcgggat atttggataa cggcggtgtt gtgagataat
     4321 tcccgccttg gatagtatgc cttcaaatat aatttccgta aaggaaaaaa aaaagaaata
     4381 ttaattttga tgatgactta acaaagcccc catttgcttg gctgtcaaag gggggtgtta
     4441 agaaaagcaa tccctctttt tgacggggtg cgatatataa aggaatggaa aatatggtaa
     4501 cgttttcaaa aatcagaccg cttttggcaa tcgccgccgc cgcgttgctt gccgcctgcg
     4561 gcacggcggg aaataatgct gtccgcaagc cggtgcaaac cgccaaaccc gccgcagtgg
     4621 tcggtttggc actcggtggc ggcgcatcta aaggatttgc ccatgtaggt attattaagg
     4681 ttttgaaaga aaacggtatt cctgtgaagg tggttaccgg cacatcggca ggttcgattg
     4741 tcggcagcct ttttgcatcg ggtatgtcgc ccgaccgcct cgaattggaa gccgaaattt
     4801 taggcaaaac cgatttggtc gatttaacct tgtccaccag tggttttatc aaaggcgaaa
     4861 agctgcaaaa ttacatcaac cgaaaagtcg gcggcaggca gattcagcag tttcccatca
     4921 aatttgccgc cgttgctact gattttgaaa ccggcaaggc cgtcgctttc aatcagggga
     4981 atgccgggca ggctgtgcgc gcttccgccg ccattcccaa tgtgttccaa cccgttatca
     5041 tcggcaggca tacatatgtt gacggcggtc tgtcgcagcc cgtgcccgtc agtgccgccc
     5101 ggcggcaggg ggcgaatttc gtgattgccg tcgatatttc cgcccgtccg ggcaaaaaca
     5161 tcagccaagg tttcttctct tatctcgatc agacgctgaa cgtaatgagc gtttctgcgt
     5221 tgcaaaatga gttggggcag gcggatgtgg ttatcaaacc gcaggttttg gatttgggtg
     5281 cagtcggcgg attcgatcag aaaaaacgcg ccatccggtt gggtgaggag gcagcacgtg
     5341 ccgcattgcc tgaaatcaaa cgcaaactgg cggcataccg ttattgacgc tatgccgtct
     5401 gaacgtgttt ccagaaggct ttatccatag aaaaaaatac ccgaatcccg tacaatacgc
     5461 ccctaaaatt ttgaaagaac aaaagaatca tggaagccga agtaatcaac cagctcaaca
     5521 ataccctgaa cgatttggaa aagcgcagcg aagacatccg agtttatatg gattatcagg
     5581 gtaagaaaga ccgattggaa gaagttatcg gtctttccga agaccccgaa ctttggaacg
     5641 acccgaaacg cgcccaagaa atcggcaaag agcgcaaaat cctcgaaggc atcgtgttga
     5701 cgctcgacaa catcgcttcg ggcatcgaag acaaccgcat gctgattgaa atgaccgtcg
     5761 aagaaaacga cgaagaaggt tttgccgccg tgcaggaaga cgtagcgggg ctggaaaaac
     5821 agatggcgga tttggagttc aaacggatgt tcaaccagcc cgccgacccg aacaactgct
     5881 ttatcgacat caccgcaggc gcaggcggta cggaagcgga agactgggcg ggtatgctgt
     5941 tccgtatgta cagccgctac gccgagcgca aaggcttcag aatcgaaatc cttgaagaag
     6001 acgacggcga aatcgcaggc atcaaccgcg ccaccatccg tgtggaaggc gaatacgctt
     6061 atggtttgct gcgtaccgaa accggcgttc accgcttggt gcgttactcg ccgtttgact
     6121 cgaacaacaa acgccatacc tcgtttgcct ccgtgttcgt ttaccctgaa atcgacgatt
     6181 ccatcgaaat cgaaatcaac cccgccgatt tgcgcatcga cacctatcgc gcatcgggtg
     6241 cgggcggtca gcacatcaat aaaaccgact ccgccgtgcg cattacccac gagccgacgg
     6301 ggattgtggt gcaatgtcaa aacgaccgtt cgcaacacgc caacaaagcc gctgcgatgg
     6361 aaatgttgaa gtccaaactg tatgaattgg aaatgcgcaa acgcaatgaa gagaaacagg
     6421 cgttggaaga aggtaaatcc gatgtaggtt ggggcagcca aatccgttcg tatgttttgg
     6481 attcctcacg tatcaaagac ttgcgtacag gctacgaagt cggcaacacc aaagccgtat
     6541 tggatggcga cttggacggc ttcatcgaag ccagcctgaa acagggcgtg tagtttaggc
     6601 tgtatgccgt ctgaaacctg taccgtttca gacggcattt ttgtgtttcg tgttataatc
     6661 cgcgccgcac acgggacggg cagacagtcg ccgcgtatcg cgtaaggcat acggggagga
     6721 aagtccgggc tccgcagggt agaatgccgg ttaacggccg ggcgcggtaa cgcgacggaa
     6781 agtggaacag aaagcaaaac cgccgatggc tgctttggca gcacaggtaa gggtgaaaag
     6841 gtgcggtaag ggcgcaccgc gcatttggta acaatatgcg gcaggccaaa ccccattcgg
     6901 agcaagacca aacagaacgc aatgacgctg cccgccgagc gttcgggtag gttgcttgag
     6961 cataccggta acggtatgcc tagaggaatg actgtccgag acagaacccg gcttaccgcc
     7021 tgtccggtgt gcatccgaca cgcaaatgtc gtctgaaacc tgaaatatgg ttcagacggc
     7081 atttttattt aggttgtgtt tttatagtgg att
//
LOCUS       DAWIVB010000113         7079 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWIVB010000113 DAWIVB010000000
VERSION     DAWIVB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.113"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(268..2958)
                     /gene="glnE"
                     /locus_tag="WIX60_001508"
     CDS             complement(268..2958)
                     /gene="glnE"
                     /locus_tag="WIX60_001508"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ5305762.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWGEIRAEENEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAELGIML
                     SEHVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETIAAR
                     LDQIRHGHKYRRLSAHAQPRFDALVPLLVRAAAAQTNPTDMLMRLFDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMSQSSWAAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     SCAWADMPKKHRDTPQFAIVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGSPEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNISADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATV"
     gene            complement(3156..3368)
                     /locus_tag="WIX60_001509"
     CDS             complement(3156..3368)
                     /locus_tag="WIX60_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305763.1"
                     /translation="MGFWNGVAKAAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIV
                     KSDGFFKNSTREKSAAYAILKERGEV"
     gene            complement(3501..3974)
                     /locus_tag="WIX60_001510"
     CDS             complement(3501..3974)
                     /locus_tag="WIX60_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ5305764.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4137..5144)
                     /locus_tag="WIX60_001511"
     CDS             complement(4137..5144)
                     /locus_tag="WIX60_001511"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ5305765.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTDNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGEDSADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            5376..5612
                     /gene="acpP"
                     /locus_tag="WIX60_001512"
     CDS             5376..5612
                     /gene="acpP"
                     /locus_tag="WIX60_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ5305766.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            5768..7015
                     /gene="fabF"
                     /locus_tag="WIX60_001513"
     CDS             5768..7015
                     /gene="fabF"
                     /locus_tag="WIX60_001513"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ5305767.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLTGKSGIGAITRFDTS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPGIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSLRMIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1602 a         1890 c         1909 g         1678 t
ORIGIN      
        1 aaaagtagaa tgcgctattt taatggaaac ggcggggttt taaacggttc ttatttgttt
       61 gttttgattt ttgcttggat aaaaaatccc atcattccca caaaaacaga agcctgaaat
      121 cccgtcattc ccgcgaaaga ggaatccggt ttttgggttt cagccattcc cgataaatcg
      181 ctttagctct gccgcaacct taaaacggca cggtacgaaa ataccgtctg aaacccagat
      241 tgtcaggctt cagacggcat tttttgttca gacggttgcc gcttcttcgc cgaacacttc
      301 ccgccacaat ttcctgacat tgccgtaatg tgccaacagt tcgccggtta cttcggtttt
      361 tgccgcgtcg cgcagtttgg tgttgtgctg ctgccggcgg tagaagcgat aggcggtgcg
      421 gctttgtccg gcgagggttt tgtcaatcaa accgcagtcg gcggagatgt ttaagagggc
      481 gatgttgccg tagttgtcca agagttgcgg atactggcgg gcatgggcaa gtatcagata
      541 ttggacgata aattcgacat cgaccacgcc accgcgcgcg tatttgacgt tgctgtcggc
      601 aggcgggtgg gtggggaaca ttttttcgcg catttcgatg atttcgcctg ccaaagcggt
      661 ttggtcgcgt tcggcggtga ggatttcggt gcggatgcgg tcgaatgccg tctgaatctc
      721 gggcgagccg cagataaaac gggcgcgggt gagggactgg tgttcccacg tccacgcgtt
      781 ttcgcgctgg tatttttcaa aggcggcaat actgtgcgca aggaaacctg cgtcgccgtt
      841 ggggcgcagg cgcaggtcgg tttcgtagag gctgcccgcg ccggtggcgg cggaaagcca
      901 gttggttagg cggcgggcga ggcggctgta cacgtcgcct gcatcgggat gcgggtcgtc
      961 ataaagatag accaagtcga ggtcggaggc gtagccgagt tctttgccgc cgagtttgcc
     1021 gtagccgacg atggcgaatt gcggtgtgtc gcggtgtttt ttgggcatat ccgcccacgc
     1081 gcacgaaagg gcggcggcga gtatggtgtc ggcaagggcg gagagctggt cggagaggga
     1141 ttctaccgtc cacagtccgg cgaggtcttg gacggcgaga cggaagactt gggcgtgctg
     1201 gaaatggcgc agggtgtcca tttgcgcttc ggtgtcgccg ccgcaggctt tgatgccgtc
     1261 tgaaagttcg gcggcgagtt tgtcccaatc aaacgccgta tctaataatt gcgcgctgat
     1321 gagttcgtcc aacagaatcg ggtatttgtt cagatacgcc gccgcccaag aactttggct
     1381 cataatctct gccagttgcg ccagggtttg cggatgttcg ttgaggaagg cgagataggc
     1441 ggagcggcgg ctgatgtttt cgaggaagtc aaacaaccgc atcagcatat cggtcgggtt
     1501 ggtttgcgct gccgccgccc gtaccagcaa cggcacaagc gcatcgaaac gcggctgggc
     1561 gtgggcggaa aggcggcggt atttatggcc gtggcggatt tggtcgagcc ttgcggcgat
     1621 ggtttcggca tcgaacccgt gcgccttcag acggcatcgc cgcccttctt cgtcgggttt
     1681 gtcctgccat gcccattgcc attcgctgtt gtcttgcgtt tgctcttcgg gttcgctcaa
     1741 aatttcgttg aacaactgat tgactttgtt ccgatgaaca ttgagaccgt ctgaaaaagc
     1801 ggaataactg tcgaaaccca tgctttcggc gagcagttgc cgctgttcgg gcgaggtcgg
     1861 cagggtttgg gtttgctggt catcccagta ttgcaggcgg tgttcaacat cgcgcaggaa
     1921 gcggtaggcg gcaagcaggg tttcgacgtg ttcagacagc atgatgccca gctcggcaag
     1981 cttcttcagc gtttcctgcg tgcctttcag ttgcagcgcg cgcatttgtc cgccgcgtat
     2041 catctggaaa atctgggcga taaattcgac ttcgcggatg ccgcccgcgc cgagtttgat
     2101 gttgtccgcc atgccttttt tgctgacttc gctgctgatt tggcggtgca gcttacgcat
     2161 cgcctcatac gcgccgtaat ccagatattt gcggaacaca aaggggcgca ccagtgcttt
     2221 gatgtcgttc ggatacggcg taaccacgcg acctttgcac cacgcgtagc gttcccattc
     2281 tcgcccctgt gtaatcaaat attgctccag cgcggtttcg ctcaatacca acgcgcccga
     2341 atcgccgtcc ggccgcagcc gcatatcgac gcggaacacc tgcccatcgg cggtaatgtc
     2401 gttcagcagc gcaatcagtt tctgcccgac tttggtgaaa aattcctgat tgccccgttc
     2461 gcgcctgccg tcggtgtcgc ctgattcggg atagacgaaa atcaaatcga tgtcggaaga
     2521 cacgttcaac tcatagccgc ccgccttgcc catcgccacc acgctcaaat gctgcggcga
     2581 tttggtataa cgcccgatcg gcgtgccgta catgtcccga taataggcgt aggcaaaatc
     2641 cagcgcggta ttgacggcaa aatcggcaaa cagcgtaatc gtgcgggtta cttcgttcaa
     2701 atcgctgata cggttgatat cgcgcacgat aatctgcgac accacataac ggcgcaactc
     2761 gcgcaactgc cgcgccaatt cttcctcgtt ttcttccgcg cggatttcgc cccagtcggc
     2821 aaaggcttgg aaatccgctt cggtcaaaac cttgtcgagc ataggcagga atatttcggg
     2881 cttgagtttg ccgttgtcga gctggcgggc gaggaagagg gaatggcggc gggcggtgtc
     2941 gaggcggttg tcggacattt cggattccgt ttggaaggat gacgggaatg agatagtgga
     3001 ttaactttaa atcaggacaa ggcgagggga tgccgtaccg gtttaaagtc aagccactat
     3061 atcatagcaa ccttcgatgt cgtctgaaac cttgccccga tattcggacg gatgcctacc
     3121 aataaaaaac agcggcaaat gccgccgttt cctgttcaca cctcgccacg ctcttttaaa
     3181 atagcataag ccgcactttt ctcccgtgtg gaatttttaa aaaaaccatc actcttgacg
     3241 atttcatgca gctcctcact tgatttctcc gcatattcca ttttcaacgc cttatgctca
     3301 ttgccggctt caatcattcc ctctcccact gcttttgctg cttttgccac accattccaa
     3361 aaacccataa tcatgtcctc caatcgtttc aaataaggtc gttgtttctg tcaagaaaca
     3421 acgacagttt ataaactgtc ggaaaacaaa atgccgtctg aaggccggat aatgcttcag
     3481 acggcatcgg attgccggtt ttaaccctgc cctccgccgt tgttcaaata gtgttcccag
     3541 tgagaatata accgggcaat attttcttca taggtgtggg cagaaggaat ataaccgtct
     3601 ttgggcggca tatacctgac aaatgattct atttcttccg gataatcaaa atctgcataa
     3661 attttttcaa cttcccccaa aggatcctca aattcttctt ttctatggaa tacatcgctt
     3721 aagaggacgt acagccaatt tttaggggaa taatccctca tttccgaagc caaatcttcc
     3781 agaacaggct ttaattcaag aatattggat ttgttgatta aagatagttt gaacacacgt
     3841 tcatcatgat ctgaaagcgt catttttcgg gcataagctg ccacatcagc ccaacccaag
     3901 tatttattcc cataccccca cagaatatcc ttccaagaaa gacggacttt ttgcgcggtt
     3961 aaatcaaggt tcatttttta ctccgattgc ctgctccgtg ttcacgcatc ggtttgcctt
     4021 atttcaaaca gggcggcgcg taagggattt tcaaggggcg tcaggcggaa aaaggaaatg
     4081 aaaatgccgt ctgaacggca aaacgtgcgt tcagacggca ttttgggcgg atcgcgtcat
     4141 cgcgccaaag ccttcaaaca ttctttgacc aatgccggac ctttgtatat caatccgctg
     4201 tacacttgga cggcggtcgc gcccaagcgg attttatctg ccgagtcctc gccttccata
     4261 atgccgccta cgccgataat cggcagcttg ccgtctatgt ggtctgccaa cagcttcaac
     4321 acccgattac ttttttcatg aacgggcagc ccgctcaaac cgccctgctc gcctgcgagc
     4381 ggatggctgc cgagacttga tttgtcgatg gtggtattgg tagcgatgat gccgtccatt
     4441 tcgacggatt tgacaacgtg ggcgatgtct tcgatttgtg cttcatccaa atcgggggcg
     4501 attttgacgg cgagcgggac gtatttcccg tgtacagagg caagctgtgc ctgtttgttt
     4561 ttcaaagcct caagcaatgc gctcaactcg tcgccacctt gcagcgcgcg gaggttttta
     4621 gtgttgggcg aggaaatatt gacggtaatg taacttgcgt gtgcgtaggc tttttcaagg
     4681 cagattaaat aatcatcggc agcgttttcg atgggtgtaa ccgcgttttt accgatgttg
     4741 atgcccaata cgccactgaa tttacttttt tcgatgttgc gtatcatggt gtcgataccg
     4801 tggttgttga aacccatgcg gttgatgatg ccttggtgtt cgggaacgcg aaagaggcgc
     4861 ggctgcgggt tgccgggctg cgggttgggc gttaccgtgc cgatttcgat gaaaccaaag
     4921 ccgagcgcgc ccaatgcgtc gatgtattcg ccgtttttgt cgagtccggc ggcaagtccg
     4981 acagggttgg gcaaatccat acccatcaat tttacaggtt tggtacggtt gtcggttaca
     5041 ggaatcaaac ccaatttata aaccgtgtag agcgcgtcga gcgtgaagtg gtgggctttt
     5101 tcggcatcga gtgcaaacag gatgcgacgg gcaagtggat acatgacagg gctttctgct
     5161 tcagatggaa atgccgccat tttaaccgaa atcccgccaa acatacggca cacaggtcaa
     5221 acttgaacga caaaacccgc cgcacccaaa aatgtacaaa tcggcaaata ttgatataat
     5281 agtcattaga tagaaaactc tattaaaagt cggtacaata ccaccgattt atttccctta
     5341 aacaacctgc cgcaaggcat aaaggaacga ctgatatgtc aaacatcgaa caacaagtta
     5401 aaaaaattgt tgctgaacaa ctgggcgtaa acgaagccga cgtgaaaaac gaatcttcct
     5461 tccaagacga cttgggcgcg gattctttgg acaccgtgga gctggttatg gctttggaag
     5521 aagcattcgg ctgcgaaatc cccgacgaag atgccgaaaa aatcaccacc gtccaactgg
     5581 ctatcgacta catcaatgcc cacaacggct aaccggtcgt cgcccgacac aacagcctct
     5641 gctgcaccgc gcaatagagg cttttccctt atatggcaaa ctgtttgaac ccctgccgca
     5701 aattacagac ggcatctgca cgacagccgc atatgccccc attccgacaa ccaacagcga
     5761 ggttatcatg agtcagagaa gagtagtcat tacaggctta ggtcaggttt cccctgtcgg
     5821 caacactgtc gcagaggctt gggacaccct gctcaccggc aaaagcggca tcggcgcgat
     5881 tacccgcttt gacacatccg acatcaacag ccgtgtcgcc ggcgaggtgc gcggtttcga
     5941 catcggacaa tacatcagcg cgaaagaagc gcgccggatg gacgtattca tccactacgg
     6001 cattgccgcc gcattgcagg caatcgccga ttcgggtttg gacgatgtgg aaaacctcga
     6061 caaagaccgc atcggcgtga acatcggttc cggcatcggc ggactgcccg gcatcgaggt
     6121 caccggcaaa gccgtaatcg aaggcggcgc gcgcaaaatc aaccctttct ttatccccgg
     6181 ttctctgatt aatctgattt ccggacacgt taccatcctc aaaggctacc gcggcccgag
     6241 ctacgggatg gtttccgcct gcaccaccgg cgcgcacgcc atcggcgatt ccctccgtat
     6301 gattaaatac ggcgacgcgg acataatggt tgccggcggc gcggaaggcg caatcagcac
     6361 tttgggcgtg ggcggttttg ccgcgatgaa agccctctcc acccgcaacg acgaccccgc
     6421 caccgcttcc cgtccgtggg acaaaggccg cgacggcttc gttatcggcg aaggcgcggg
     6481 catattggtg ttggaagaat tggaacacgc caaaaaacgc ggcgcgaaaa tctacgccga
     6541 aatcgtcggc ttcggcatga gttccgatgc ttaccatatc accgcgccga acgaagaagg
     6601 ccccgccctt gccgttaccc gcgcgctgaa agatgccggc atcaatcccg aagacgtgga
     6661 ttacgtcaac gcgcacggca cgtccacccc cttgggcgat gccaacgaaa ccaaagccct
     6721 caaacgcgcg ttcggcgaac acgcctacaa aaccgtcgtc agctcgacca aatccatgac
     6781 cggccacctg ctcggcgcgg cgggcggcgt ggaggccgtg tacagcattt tggcgataca
     6841 cgacggcaaa atcccgccga ccatcaacat ttttgaacaa gacgttgaag ccggctgcga
     6901 tttggactac tgcgccaacg aagcgcgcga cgcggaaatc gacgttgcca tttccaactc
     6961 cttcggcttc ggcggcacca acggcacgct ggtcttcaaa cgcttcaaag gctgattccg
     7021 caaagccgcc gccgacatcg aaatgccgtc tgaaaccgtt tcagacggca tttttatag
//
LOCUS       DAWIVB010000114         7073 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWIVB010000114 DAWIVB010000000
VERSION     DAWIVB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.114"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(428..1120)
                     /locus_tag="WIX60_001514"
     CDS             complement(428..1120)
                     /locus_tag="WIX60_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ5305768.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
     gene            complement(1117..1860)
                     /locus_tag="WIX60_001515"
     CDS             complement(1117..1860)
                     /locus_tag="WIX60_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ5305769.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLVRKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            complement(1889..2452)
                     /gene="gmhB"
                     /locus_tag="WIX60_001516"
     CDS             complement(1889..2452)
                     /gene="gmhB"
                     /locus_tag="WIX60_001516"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ5305770.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            complement(2700..3751)
                     /locus_tag="WIX60_001517"
                     /pseudo
     CDS             complement(2700..3751)
                     /locus_tag="WIX60_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(3901..5142)
                     /locus_tag="WIX60_001518"
     CDS             complement(3901..5142)
                     /locus_tag="WIX60_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ5305771.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGDVSLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWAAGGLIADAKPSVLFDTQAPAGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSISGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            5294..6022
                     /gene="ubiG"
                     /locus_tag="WIX60_001519"
     CDS             5294..6022
                     /gene="ubiG"
                     /locus_tag="WIX60_001519"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ5305772.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            6059..6976
                     /gene="thrB"
                     /locus_tag="WIX60_001520"
     CDS             6059..6976
                     /gene="thrB"
                     /locus_tag="WIX60_001520"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ5305773.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGKFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLG"
BASE COUNT         1406 a         1671 c         2328 g         1668 t
ORIGIN      
        1 gatgcaggtt ttcttaaccc cgcgttctag attcccactt tcgtaggaat gacggcggta
       61 gatttggcag atgcggcgga ttcggcaggt ctcaacccat cctacaatcc accctgatcg
      121 ccttttacga atccgccgcc atcctgggaa acgcaaaaaa aatgccgtcc gaaaaccttt
      181 cagacggcat tttcgcgggc aaatcagtaa aagacttcgc gtcagcttaa gcgtttcata
      241 ccgcacgtcg gaccgggcgg gtttcgggtt tcggaagagc ggtgtgaaat gaaacgcggc
      301 agacggcgtg tgtgccgcca tcctgccccg aaccggcggc aaagcgtcat cgcggtttga
      361 atcgggcggg cgcgtgcctg ttccggcacg gatgccgtct gaaagcggcg ggtcggtccg
      421 gtccgcgtca gcctaaggcg aaaagttccc tgccgtggat tttgagccac tgtttcgctt
      481 tgggcgtgta gggggcgaag cggcgggtca gttcccaaaa ggcggggctg tggtcgggat
      541 gggcgaggtg gcagagttcg tgtatgcaga catagtcggc aacgtattcc ggtgcgccga
      601 ccagccgcca gttgaagcgt atgcctgtgg ttttgcggca cacgccccag aaggttttgg
      661 cagaggtcag cgaggaggag gcggggaaca gttgtgtggt gcgggcgtgg cgttcgaggc
      721 ggggaatcag gtaactgtgc gcctgccgtt ccaaaaagtc ccgcagcagc gcaagctgtt
      781 tttcgggtgc gccttcggga acacggattt cagacggcat cagcaggatt tgcgtgtctt
      841 gatgggcggt gagggcaagc tgtctgccgt ggaagaggat ggattcgggc agccggtttt
      901 cggcagtttg cggcggcggt gttttcgcca gtgtttgccg caggacggct tcgttttcat
      961 acagccagcg gtttagagcg gagacggaga agcagggtgg gacgctgatg cggacggtat
     1021 gtgtgccggc ggggcggata atcaggtttt tcttggcacg gcgcttgatt tcgacggtca
     1081 gttccatgcc gtctgaaagg gtgtggacaa aggcggtcat gcggtttcag acggcatttt
     1141 ggcggcaaac gggcctgcgc cggaaataag cggttgttgc gtttcgatga gatgttcgca
     1201 tttttccatc aattcggctt cgctgccgct tgcgtgcggg atggtcggac agatgacgac
     1261 ggtgatttcc cccggatatt tcagaaagga gtttttcggc caaaattcgc cgctgttgag
     1321 ggcgacgggg acgatgtcca tctcaaacat tttcgccatg cgcgcgccgc cgagtttgta
     1381 tttgccgcgt tttccgggcg caaggcgcgt gccttcgggg aaaatggtaa tccaatagcc
     1441 ttcgtttttg cgcaccaacc cctgttttat gagctgctcg ttggcttcgc ggcggttgtt
     1501 gcggtctatg cctatggttt tgaccagttt caagccccag ccgaaaaagg ggattttgaa
     1561 caactcgcgt ttggcaacgt aaacctgcgg cggaaaaatg tcctgaaggg cgagcgtttc
     1621 ccagccgctt tggtgtttgg cgcagatgac ggcggggcgg tcggggatgt tttccgcgcc
     1681 gatgatgcgg tatttgagcc cgacgatgtg tttgagcgac cagttgagaa tgccgaccca
     1741 gacccgcgcc atcttgtgcg ccccgtcccg gaaaggcgag gcgagcagca taaagggaaa
     1801 gaggaaaatc agggtggaac agagtatcag ccagtaaatc aggttgcgga tgatgagcat
     1861 ggttttgcct tgtcggaatg cggtatgttc agtcggcttg cggtgcggtg ttttcctgca
     1921 tgatgtattg tgagaaatcg agcagggtat cgaaaacctg tgtgtgttcg ggcaattcgt
     1981 gtccgtgttg ggagagcgtt tttttgcctt ttccggtcag aaccagcgcg ggtttgccgc
     2041 cgacggcatc gattgcctgc aaatcgcgca ggctgtcgcc gaccagccag gtttccgagg
     2101 cttgggcgtt gaagcgtccg atgatgtctt caatcatacc cggtttgggc ttgcggcagt
     2161 tgcagttgtc ggcatcggtg tgcgggcaga accagatgcc gttgatttcg ccgcctgcct
     2221 gacggacgag gcggtgcatt ttggcgtgca tttcggtaag gttttgaacg gtaaaatatt
     2281 tgcgcccgat gccggattgg ttggtggcaa cggcgacggt gtagcctgcc tgcgtcagaa
     2341 atgccaccgc atccatgctg ccttcgacag gtatccactc gtcaacggat ttgacgaagt
     2401 cgtcgcggtc ctgattgatg acgccgtcgc ggtcgagaat gatgagtttc atcgcgtttc
     2461 ctttggattg gggcaggtcg ggggtggcat tatactgaaa tatcggtgga aatgcgcctg
     2521 tgccgcgcga taacgcgcct gttccggcaa ccgcttgcaa tgcgcgggta cggcgtttcg
     2581 ggggcggttg ccgatgtttt cctgcctgct cctgcaacgg cggattttct gccggacgta
     2641 ttttccgctg caaaagtctc ggcgcggaag ttgcggggca atgccgtttg gggacgcggt
     2701 caggcggcaa gcatttcaag aatcagcccg atgtgtttgt ctaaagccgg gttgtagttc
     2761 agtgcccgca tcgggtcgga caggcggtgt ccgccctgtt ttttaaggct gacggcttgg
     2821 gcaacggctt gcgccagggt ttccgggttt tggcgtgaga aaaagaagcc ggcttcttcg
     2881 ttcatgacct ctgtacatgc catgttttcg gagaggacga cgcgtgtgcc gcataggacg
     2941 gattcgacgc cgaccagccc gaagggttcg tacagggaag ccataatggt aaagtcggcg
     3001 gcgcggtaga gttcgggcat atcggtgcag aagcccagtc cgacgacgtt tttcataggg
     3061 cggggaagcg gggagccgac aacggcgagc ttgacgggca ggctggtatg ttcgaaaaag
     3121 tcggcaagca gttccagacc tttgcgcgtg tggccggtcg atgggaacag gaaaacggtt
     3181 tcatggtcgg caaagccgta tttggcgcgc aggtcggcag tttctccggg ttgtggaaag
     3241 aagcgttccg tatctgcggg gggggggcga cttggattct ttcaggggga acgccgtaca
     3301 gtccgaccag ttcgcgccgc atcatatggg aatgcgccat aatcagtttg gcggtggcgt
     3361 agttgctgcg gttgcggcgt atggcgaggc ggtcgagcag gttcggtttt tgcgccatat
     3421 ggtgcaggta gcccaagtgt gtgccgccgc agatgaggag gtcggcgtaa tcggcgtggt
     3481 ggcaggcaat cagtttggcg gcactgtttt ttctggtttg agcgagccgg cttgaaaaga
     3541 ggaatgagcg tagttttttc agcgtccggt gttgatcgac aagatggggt tcgattaggg
     3601 cgtattcagg aatgctgtga tcaaatttcg tcgcataaac ggccggtgtg atgttttgtc
     3661 tgttcagacc ctttaccaaa tccaatgtgt agcgttccgt gccgccgccg tgtttgaagt
     3721 tgttggttgc aatgtctatt ttgagcttca tcattgttcc tttatggttg cgtcccggtt
     3781 tgtcggggcg ggatttgtgc gtgaggggca gggtaatgcg ctgtgtgccg gaatacggtt
     3841 gccgtttgtt gcggcaatgc cgtctgaagc cgccggcggg cttcagacgg cattttgcct
     3901 ttatccttta aatacgggga cgagttccat ttggctcaat acctgtgcgg cgatgttgac
     3961 gatgccgaaa aggaagaccc aaaccatcag ccacaagccg ccgtaaactt tataggtttt
     4021 gcctgcgccg aattttttgc gcgaacggta gagcagcatg gcggggatga tgcctgtcca
     4081 gacggttgcc gccaggccga cgtagccgat ggcggtaacg aagccggtgg ggaagagcag
     4141 gcaggaaatc aggggcggca ggaaggtcag cgcggcggtt ttggtgcggc cggagatgct
     4201 gtcgttccat ttgaagatgt cggcgatgta gtcgaagagt ccgagcgtta cgcctaaaaa
     4261 cgaggtggcg atcgccatat aggaaaacag ggacaatatt ttgtccatat tgccggtttg
     4321 ggcgaatttg gacagggttt cgatgaggac ggagacttgc ccttcggcgg cgatgacggg
     4381 ggcgaactcg ttgcgcggca ggttgccttg gatggcggtt tgccagagga cgtaaattac
     4441 cagcgcaatc agtgtgcccg tccagatgga tttagccact ttgggcgcgt cgcctttaaa
     4501 gtatttgagc aggctggaga cgttgccgtg gaagccgaag gaagcgaggc agacgggcag
     4561 ggcggtggcg gcgtaaatcc agtagtttgt gccggcgggg gcttgggtat cgaagaggac
     4621 ggacggcttg gcatcggcaa tcagcccgcc ggccgcccaa ataaaggtca ataccatgcc
     4681 gccgataagg acgccggtga agcggtcgac caagcgtgcg gatgcccata cgcaaaaggc
     4741 gaggatgccg aagaagacga gttgtccgac ggtgagtgaa acgtcgccgc ctgccgcgct
     4801 gcctaagcct ttggcggtca ggtcgccgcc gacgaagata taagcgtaag taagcaggta
     4861 taaaacgaag gcgacggcga tgccgttgat gatgttccag ccgcgtccga gcaggtcttt
     4921 gaccatcgtg tcgaaacttg cgccgtgcgg ataatgggtg ttgacttcca aaatcatcag
     4981 gccgctggaa agcatagaaa accaggtgta cagcaacacg gccagcgagc cggtaaacca
     5041 tacgccggat gtggcggtcg ggttggcgag catgcctgcg ccgatgaccg tgccggcgat
     5101 aatcatcgcg ccgccgaaca gtgaaggggt tttgttgggc atattttgtc tttctgccag
     5161 aaaaagcgag ccgccattat gccgtaaagt gtaaggattt gtaaggtatt tgcgccgcgc
     5221 cgcccgaaaa ggctttcaga cggcattgtg ttccatagta taatcttggg ttttggagtg
     5281 ggcggttcgt cagatgggag ggaaaatgtc cgacaaaaaa tataatgtcg atgaggggga
     5341 aatcgccaaa ttcagccgga ttgccgacaa atggtgggac aagtcgggcg agttcaaaac
     5401 cttgcacgac atcaatccgc tgcggctgga ttatatcgac ggacacgcgg atttgtgcgg
     5461 caaacgggtt ttggacgtgg gctgcggcgg cggcatcttg gcggaaagta tggcgcggcg
     5521 cggcgcggcg tttgtaaagg gcatcgacat ggcggagcag tcgttggaaa ccgcccgcct
     5581 gcacgcggct ttgaacaatg tcgccgatat cgaatacgaa tgtatccgcg tggaagacct
     5641 tgccgaggcg gaaccgcact cgttcgatgt ggtaacgtgc atggaaatga tggaacacgt
     5701 ccccgatccc gccgccatcg tgcgtgcttg tgccaatctg gtcaaaccgg acggcatggt
     5761 gtttttttcc accatcaata aaaacccgaa atcgtacctg catctgattg tggcggcgga
     5821 atatctgttg aagtttgtcc ccaaaggcac gcacgactgg aaaaaattca tcgcacctgc
     5881 cgagctggcg cgaatgtgcc gtcaggcagg cttggatgtg gcggatacga agggtatgac
     5941 ttaccatgtg ttgtcgcaaa cttatgccct gtgcgattcg accgatgtga attatatgtt
     6001 tgcctgccgt ccggcgttct gacggcgggt ttgcccgttt ttgagcaagt gagttgatat
     6061 gtctgtctat accagtgttt ccgatgatga aatgcgcggc ttcctgagcg gttacgattt
     6121 ggggaaattt gtttccctgc agggcatcgc gcaggggatt accaacagca attattttct
     6181 gacgacgact tcgggacgtt atgtgctgac cgtgtttgaa gtgttgaaac aggaagagct
     6241 gccgtttttt ctggagctta accggcattt gagtatgaag ggcgtggcgg ttgccgcgcc
     6301 ggttgcgcgc aaagacggcc ggcttgattc cgttttggcg ggtaagcctg cctgcctggt
     6361 tgcctgcctg aaaggttcgg ataccgcgct gccgacggct gagcagtgtt ttcataccgg
     6421 tgcgatgttg gcgaaaatgc accttgccgc cgccgatttc cctttggaaa tggaaaaccc
     6481 gcgttacaat gcgtggtgga cggaggcgtg cgcccggctg ctgcccgtcc tgtcgcaaga
     6541 cgatgccgca ctgctgtgtt ccgaaatcga tgcgttgaag gacaatctcg gcaatcatct
     6601 gccttcgggc atcatccatg ccgatctgtt taaagacaat gtgttgcttg acggcggtca
     6661 ggtatcgggc ttcatcgatt tctattacgc ctgccggggc aattttatgt atgacttggc
     6721 gattgcggtc aacgattggg caaggacggc ggacaataag ttggatgagg cgttgaaaaa
     6781 ggcgtttatc ggcggttatg agggcgtgcg ccccttgagt gccgaagaaa aggcgtattt
     6841 cccgaccgcc caacgtgccg gctgcatccg tttttgggtg tcgcgcctgt tggattttca
     6901 ttttccgcag gcgggcgaga tgacgtttat caaagacccg aacgcgttcc gcaacctgct
     6961 gttgagtttg ggttgagtgc gtccggcgtt tgacagaaat gccgtctgaa agggtttcag
     7021 acggcatttt tatggctgat taaaacgaaa atgagacgat agccgggtat ttt
//
LOCUS       DAWIVB010000115         6882 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWIVB010000115 DAWIVB010000000
VERSION     DAWIVB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.115"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(166..477)
                     /locus_tag="WIX60_001521"
     CDS             complement(166..477)
                     /locus_tag="WIX60_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ5305774.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            1006..3285
                     /gene="nrdA"
                     /locus_tag="WIX60_001522"
     CDS             1006..3285
                     /gene="nrdA"
                     /locus_tag="WIX60_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ5305775.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            3343..4356
                     /gene="dcm"
                     /locus_tag="WIX60_001523"
     CDS             3343..4356
                     /gene="dcm"
                     /locus_tag="WIX60_001523"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ5305776.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            4349..5536
                     /locus_tag="WIX60_001524"
     CDS             4349..5536
                     /locus_tag="WIX60_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ5305777.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            5597..6730
                     /gene="nrdB"
                     /locus_tag="WIX60_001525"
     CDS             5597..6730
                     /gene="nrdB"
                     /locus_tag="WIX60_001525"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ5305778.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
BASE COUNT         2035 a         1451 c         1535 g         1861 t
ORIGIN      
        1 tctgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatattaagg
       61 ctgcggcaac gggaggacct caaacgaaat ggcgtaagga tagtttgttg tatctatgga
      121 aaatgccgtc tgaacctgtg ttcagacggc atattttata tgggattaac ggcggaaact
      181 gccgccgtcg aaatcatcga aagcctcata agccgcccgg cattggattt tctgttccgc
      241 ataaggcagg gagcggaatg ctttgcgcgc ctttttgttt tcgcgcaatt cctgcacgtt
      301 gccttgcgat cggtaccacg cggcacggcg ttccaaatat ttcctgcatt cggggtgcag
      361 catctgcttg tttgccttgc ggatatgttt ggtatcgcgg tgggcgggtt ctgacgtacc
      421 ggcagcggca gttgtcgata ccagtcccaa tattgcagac aacagtatgg caagcatctt
      481 tttgacagac atgacgatcc tttcagtttg tcattctttc cagtatagga tggaaggatg
      541 gaacgggcaa attgtcggca gtttcagact cattcagccg acaaaacttg ccgtttgata
      601 tgcagattca aataacacat taatctcaat aataaattcc aatcggtaag aaaatggaat
      661 ttgtcggcgg cggcgcggca aaatcatact ttgcaaaatt taacaatttg caggggcaga
      721 aaacaggaag ctttcctttt tcgtcggaaa atccttattt caccgccttg tagccggagc
      781 cggtcaaaag gcaaaaaatt tacccgtttt ttatcggtaa agaattatca gataaaacaa
      841 atattatagg aaaaatacga caggcgggtt ttatcgcgca ttgcctgaaa ctgaaaaata
      901 caaccgttgt caagactgga gaaaatgcca aaaatccact atattgtctg ccttaattta
      961 tttgaaaaga ctgtgtcttg aatatcaaga gtggaagagg aagcgatgaa tacaccgact
     1021 gatttgaaag taaccaaacg agacggaaga ttagaagcca ttgatttgga taagattcac
     1081 cgtgtcgtca cttgggcggc ggacggattg gaaaatgttt ccgtgtcgca ggtcgagttg
     1141 aaatcgcaca tccagttcta caacggcatc cgcaccgacg acatccacga aaccatcatc
     1201 aaagccgctg ccgatttaat ttcggaagat accccggact accaatacct tgccgcgcgt
     1261 ttggcgattt tccatcttcg taaaatagcc tacggcgagt acgagccgcc gcacctttac
     1321 gaccacgtta aaaaacttac cgatgccgga aaatacgaca ggcatatcct tgaggattac
     1381 agccgcgaag aatttgacga actgaacgcc tatatcgacc acgaacgcga tatgtccttt
     1441 tcctatgccg ctgtcaaaca gctcgaaggc aaatatctgg tacagaaccg cgttacccgc
     1501 caaatttacg aaacgccgca gtttttatat gttttggtgg cgatgtgcct tttcagcaaa
     1561 tacccgaaag aggcgcgctt gggttacgtc aaacggtttt acgatgccgt ttctacattt
     1621 aaagtatcgc tgccgactcc gattatgagc ggcgtgcgta cgcctacgcg ccagttctca
     1681 agctgtgtgc tgattgaatg cgacgatagt ttggattcca tcaatgccac taccagcgcg
     1741 attgtgaaat acgtttccca gcgtgcgggc atcggcatca atgccggacg tatccgcggt
     1801 ttggacagcg aaatccgggg cggcgaagcg cggcataccg gctgcattcc cttctttaaa
     1861 atgtttcagg cggcggtcaa atcctgttcg caaggcggcg tgcgcggcgg cgcggcaacc
     1921 ttgttctacc ccttgtggca tatcgaagcc gaaagcctgc tggtgttgaa aaacaaccgc
     1981 ggtgtggaag acaaccgtat ccgtcagctt gattacggcg tgcaaatcaa ccgcctgctg
     2041 tacacccgcc tgattaaggg cggcaacatt acgctgtttt cgcccaacga ggttccggga
     2101 ttgtacgaag cgttttttgc cgaccaagac gaatttgagc ggctctatac gaaatacgag
     2161 caagaccctg atatccgcaa gcgcatcatt ccggctgccg acctgttttc cacgctgatg
     2221 caggagcgtg ccggaaccgg gcgcatctac attcaaaacg tcgatcactg caatacgcac
     2281 agcccgttcg atccgcgcgt cgcgcctgtt catcagtcca acttgtgtat ggaaatcgcc
     2341 ctgccgacca aaccgctgga caatatcaac gatccgaacg gcgaaatcgc cctgtgtaca
     2401 ctgtctgcct ttaacttggg cgcattaaac agcttggacg aattggaagg gcttgccgac
     2461 ttgaccgttc gtgcgctcga tgcactttta gattatcagg gatatccggt agaagccgcg
     2521 cgtacctcta ctatgggccg ccgttcgctc ggcatcggcg tgattaacta tgcttattat
     2581 ctggcgaaaa acggtgtccg ctacagcgac ggttccgcgc tcggtctgac ccaccgtacc
     2641 tttgaagcca tacagtatta cctgctcaaa gcatcggcaa accttgccaa agaatacggt
     2701 gcgtgcacgc tctttaacca aaccgtttat tcgcaaggca aactgcccat cgacacttac
     2761 aaaaaagatt tggatgccgt ctgcggcgag cctttgcatt acgactggga aagcctgcgt
     2821 gccgaaatcg tcaaatacgg cctgcgcaac tctactctga ccgcgctcat gccgtctgaa
     2881 accagctctc aaatcgccaa cgccaccaac ggcatcgagc cgccgcgcgg attggtaacg
     2941 gtcaaagcat cgaaagacgg cattttgaaa caagtcgtgc cggagtttga aaccctgaaa
     3001 aatgcctatg aaaccctgtg gcagcttccc ggcaacgaag gctacctgaa acttgtcggc
     3061 gtgatgcaaa aattcgtcga tcaatcgatt tccgccaata ccgcctacga cccgggcaaa
     3121 ttcgaaggcg gcaaagtttc tatgaaacaa atgctcaaag acctgctgac cgcctacaaa
     3181 tacggcgtca aaaccctgta ctaccataac acccgcgacg gtgcggacga tacgcagacc
     3241 gatattcagg atgacggctg cgcgggcggg gcttgtaaga tttgatgaaa gggggagttt
     3301 tcagatggcc tttagattaa taaatcatct gaaatataaa atatgaaaaa taaaatacaa
     3361 aatcaattag ataatatagt taccttaaat aatgagaaat ttacttttat tgatttattt
     3421 gcgggaatag gtggttttcg cattgcaatg gagaatgttg gtgggcgatg tgtattttct
     3481 agtgaatggg atgataaagc ccgtcaaacc taccaagtaa attttaatga tattccttat
     3541 ggagatatta cattaaagga aaccaaagca gctattccaa gtaagtttga tgtattaaca
     3601 gcaggattcc cgtgtcagcc attctctata gctggtgttt caaagaaaaa aagcctagga
     3661 cgagaaacag gcttcttaga taaggcgcag ggaactctat tttttgatgt tgctgaaatt
     3721 attggaaaac atcgacctaa aatttttctt ttagagaatg tgaaaaacct tgtttcgcat
     3781 gacaaaggaa atacatttaa agtaattaaa gggactttag aagagcttga ctatcagata
     3841 ttttatcaag ttatgaatgc aaaatattat gttcctcaaa atagggagcg tatttttatt
     3901 gtaggttttg atagacaata ttttaataag gaaataaatt tcaattttcc ttccccacca
     3961 gaatcacaac caaaattaaa gcaaattttg gaagatgatg tagataattc ttttactctt
     4021 tctgataact tatggcttta ccttcaaaat tacgctaaaa aacataaggc aaagggtaat
     4081 ggatttggtt ttggattagt tgatttagat ggaatatcac gaactctatc tgcacgatat
     4141 tacaaagatg gttccgaaat actcatccct cagaaaggaa aaaatcctag gaagctgaca
     4201 cctagggagt gctcgcgttt aatgggattt cctaaagatt ttgttattga tgcagtatcc
     4261 aagacagctg catacaagca gtttggcaat tcaattgctg taccgttggt tcaagctatt
     4321 gctaaacaaa ttataaatga gttaaaaaat gaatgacttg gcagctcaga caatacaagt
     4381 agtaaataaa tccgatgact tagtagcttc agcaatacag acagcaaata aatcaattgc
     4441 agtatactgt cgttatattc gtcccaatga tgttggtact actggtagtc accaatcagg
     4501 attttatatt caaaaacatt tttcagacga cctctttgat gtagtttgcc aaaagggaac
     4561 aaataaaact atttcaatca aaattaattg gcaagatgga agcgtcacta atagcaattt
     4621 taaatattac ggccaaggca cgagaaatga ggcgagaatt acgggttttg gcaagaattt
     4681 tgaattttta agtgataaat atagtggttc cttattggta ttgtgcagag cttgttataa
     4741 agatttatta ttccatgctt ttgttttatc ttcagatgaa gatattgaaa tatttatcgc
     4801 agaaactaac attttaccag gaagtttata tttacctaaa aaacaagaag tagaggataa
     4861 cctaacaaaa ttattttcac tatttcctaa cttccccaaa actgaagaaa tggcagtttt
     4921 ggcaagggaa aatattgcat taaataaaac taacgttagt aatgtgttaa aacaatgggt
     4981 ttcaaaggaa tatgaattat tttccatttt tgaaaaacgt gaatttgaaa ttattaaatc
     5041 aaaaattact gatttggata gcttcattaa ttttgcacat tcttttacaa atcgcagaaa
     5101 agcaagagca ggaaaatctt tagagttaca tttatcaaga atatttgacg aattttcctt
     5161 aaaatttgag acgcaagcga agacagaagg gaaaaagaaa ccagattttt tatttcctgg
     5221 aagtgaagag taccatgcga tggatgaatc agggaatttc atatttcaaa ctgaaaagct
     5281 gacaatgcta ggttcaaaga ctacttgtaa ggatcgctgg cgccaagttt taaatgaagc
     5341 agatcgaatt cctcataagc atttatttac tctacaagaa ggtatatctg atactcaaat
     5401 tcaggaaatg agtgatgaga atttaacctt agttgtccct aaagaatccg taaaaacatt
     5461 tggaactttt gggaaaaccc atgttttaac tttagaaaat tttattaaat atataaagtc
     5521 tcagcaggtt agttaaaact tacagatcat ttttaattaa tatagagaat agattatgtc
     5581 ctgcgaacac ttaactatgt catacagcac ctttcccaaa accaaaaacg acgcgctgaa
     5641 tgagccgatg ttttttggtc agccggttaa tgttgcccgt tatgaccagc agaaatacga
     5701 agtatttgaa aaactgattg aaaaacaatt gtctttcttc tggcggcctg aagaaatcga
     5761 tgtctcgcgc gaccgtatcg actacgccaa tctgcccgaa cacgaaaaac atattttcat
     5821 cagcaatctg aaataccaaa cactgctcga ttccatccaa gggcgcagtc cgaatgttgc
     5881 cttgctgcct ttggtttcga ttcccgagtt ggaaacgtgg attgaaacgt ggagcttcag
     5941 cgaaaccata cactcgcgca gctataccca catcatccgc aatattgtga atgatccgtc
     6001 ggtcgtgttt gatgatattg tcgaaaacga atacattacc gcccgcgccg aagacattgc
     6061 ctgctattac gatgacttaa tcgaatacac ccagtattac aacctgttgg gcgaaggggt
     6121 gcacaatgtc ggcggcaaac ccgttaccgt gtctttgcgc gggttgaaga aaaaactcta
     6181 tctctgcctg atgtgcgtca acgtgttgga agccatccgt ttctacgttt cattcgcctg
     6241 ctcgtttgct tttgccgagc gcgagttgat ggaaggcaac gccaaaatca tcaaactgat
     6301 tgcccgcgac gaagccctgc acctgaccgg cacgcagcat atgcttaatc tgatgcgttc
     6361 tggtgttgat gattctgaaa tggcagaaat tgccgccgag ttgcaggacg aatgtttcca
     6421 actcttcaaa aaagcggcgg aacaggaaaa agaatgggcg gcatatttgt ttaaagacgg
     6481 ttcgatgatt ggtttgaaca aagaaatctt atcccaatac gtcgaatata ttaccaatct
     6541 gcgtatgcag gcggtggggt tgccggccgg atttgaaggc gcaaatcaaa acccgattcc
     6601 gtggattaat gcgtggctgt cgtccgataa cgtacaggtc gcgccgcagg aagtggaaat
     6661 atcctcttat ttgatcggtc agatcgattc tgaagtgaat acggatgatt tgggcgattt
     6721 tgagttgtaa atgttttttg aaaaaatgcc gtctgaagcc ggatgtttca gacggcattt
     6781 gtatgttgta tgatgggaaa aacagcgggt aggatcggtt attgttcctt ttatagtgga
     6841 ttatagtgga ttatagtgga ttatagtgga ttaacaaaaa tc
//
LOCUS       DAWIVB010000116         6840 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWIVB010000116 DAWIVB010000000
VERSION     DAWIVB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.116"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WIX60_001526"
     CDS             complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WIX60_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ5305779.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RTATYADFTDTDGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1554..1709
                     /locus_tag="WIX60_001527"
     CDS             1554..1709
                     /locus_tag="WIX60_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305780.1"
                     /translation="MKKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1706..2137
                     /locus_tag="WIX60_001528"
     CDS             1706..2137
                     /locus_tag="WIX60_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ5305781.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARCFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISAILIL
                     LFAVFLVWSGYPATAASLAGGTVFALAGAFVIGRSRDQGKN"
     gene            2271..6644
                     /gene="app"
                     /locus_tag="WIX60_001529"
     CDS             2271..6644
                     /gene="app"
                     /locus_tag="WIX60_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ5305782.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFTYKIVKRNNYKAGTKGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFND
                     DNNGTGKINAKHEHNSLPNRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTKTDISGNVDLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     NLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFR
                     FMSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFT
                     LQNEHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALI
                     AAGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATT
                     AFPRARRARRDLPQLQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFA
                     EDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENT
                     FDDGIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGGKIRRRVLHYGIQ
                     ARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPA
                     QHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAA
                     AAKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1832 a         1965 c         1668 g         1375 t
ORIGIN      
        1 aaacggatcg gattcccgtt tttatgggat aacggaatgt tcagccggac accaccacga
       61 tgctttcgat ccgtttattt tccgatacaa aacctcgaaa aaatcacgcc gagcaggtcg
      121 tccgccgtaa actcgcccgt gatttcgccg catgcgacct gcgccaagcg caagtgttcg
      181 gcaaacagct cgatttgatg gttgccgcac aatgccgcca gcgacaattc ttcctgcgct
      241 gctttgagtg cgttgacgtg ccgcgtccgc gccaaaaaca acccttcgct ttcgccctgc
      301 caaccggcct cgcgcaacaa cgtccgtttc agcgcgtcca agccgtcgcc ggttttcgcc
      361 gacaacgcga tgacggtttc cgcgcccgta ccgaacccgc ctgccgcgtg tgcgtgcaaa
      421 tcggatttgc tgtggatttc gatgcgtttc aactccggcg gcaacgcgtc caaaatcgcc
      481 cgtgtctttt cattcaaacc ctcgcgcgga tcgaccaaca ccagcgcgac atcggcttcg
      541 gatacggctt tgcggctgcg ttcgatgccg atacgctcga ccacgtcgtc cgtctcgcgc
      601 aaacctgccg tatcgacaat atgcaccggc acgccgtcaa tcaggatacg ttccctgacc
      661 gcgtcgcgcg tcgttccggc aatatcggta acaatcgcca cttcgtcgcc cgccaacgcg
      721 ttcagcaggc tggacttgcc cacattcggc gcgccgacca atacgacatt cagaccttcg
      781 cgcaaaatcg cgccctgctg cgcgttggca agcacatcat ccacggcgcg gcgcaagccg
      841 tccagtttgc cgcgtgcgtc tgccgcttcg agaaaatcaa tgtcttcctc gggaaaatct
      901 aacgtcgctt cgaccagcat ccgcaaggta atcaagtctt cgaccagacc gtgtatccgc
      961 cgcgaaaaat cgcccttgag cgagcgcaaa gccagacgcg ccgccgaacg gctggatgcg
     1021 tcaatcaaat ccgccacgcc ttccgcctgt gccaagtcca gtttgtcgtt caaaaacgca
     1081 cgcttcgtaa actcgcccgg ttcggcaagg cgcgcgccca attccaaaca gcggttcagc
     1141 agcatatcca tcaccaccgg cccgccgtgt ccctgaagct cgatgacatc ttcacccgta
     1201 aaacttgccg gtgcggcaaa aaacagcaaa agcccgctgt cgattgcctg tccgtccgtg
     1261 tccgtaaaat cagcataggt tgcggtacgc ggcttgggcg ttttcccgca caaagcctgc
     1321 gccatcggca gcaggttttt ccccgatatg cgtatcacgc ccacgccgcc gcgccctggt
     1381 gcggtagcga ctgccgcaat cgttggaacg ttatccgaca taaaaccccc gaaaattcaa
     1441 aacagccgcg attatagcaa atgccgtctg aagtccgacg gtttggcttt cagacggtat
     1501 aaaaccgcaa aaatgcttga taaatccgtc cgcctgacct aatatataac catatgaaaa
     1561 aacgaaacac atacgccctc ctgctcggta taggctcgct gctgggtctg ttccatcccg
     1621 caaaaaccgc catccgcccc aatcccgccg acgatctcaa aaacatcggc ggcgattttc
     1681 aacgcgccat agagaaagcg cgaaaatgac cgaaaacgca caggacaagg cgcggcaggc
     1741 tgtcgaaacc gtcgtcaaat ccccggagct tgtcgagcaa atcctgtccg acgagtacgt
     1801 gcaaataatg atagcccggt gtttccattc gggaccgttg ccgccgccgt ccgacttggc
     1861 gcaatacaac gacattatca gcaacggggc agaccgcatt atggcaatgg cggaaaaaga
     1921 acaagccgtc cggcacgaaa ccatacggca agaccaaacc ttcaacaggc gcgggcaact
     1981 gtacggcttc atcagcgcca tcctgatact gctttttgcc gtcttcctcg tatggagcgg
     2041 ctaccccgca accgccgcct cccttgccgg cggcacagtg tttgccttgg cgggtgcttt
     2101 cgtgattgga agaagccgag accaaggcaa aaattaatga caaatcctag ggcgtgcttc
     2161 atatccgccc gaacgccgaa ccgcacatat aggcacatcc cgcgcgccgc ccggaagcgg
     2221 aagccgcgcc ctcccaaaca aacccgaatc ccgtcagata aggaaaaata atgaaaacaa
     2281 ccgacaaacg gacaaccgaa acacaccgca aagccccgaa aaccggccgc atccgcttct
     2341 cgcctgctta cttagccata tgcctgtcgt tcggcattct tccccaagcc tgggcgggac
     2401 acacttattt cggcatcaac taccaatact atcgcgactt tgccgaaaat aaaggcaagt
     2461 ttgcagtcgg ggcgaaagat attgaggttt acaacaaaaa aggggagttg gtcggcaaat
     2521 caatgacaaa agccccgatg attgattttt ctgtggtgtc gcgtaacggc gtggcggcat
     2581 tggtgggcga tcaatatatt gtgagcgtgg cacataacgg cggctataac aacgttgatt
     2641 ttggtgcgga aggaagaaat cccgatcaac atcgttttac ttataaaatt gtgaaacgga
     2701 ataattataa agcagggact aaaggccatc cttatggcgg cgattatcat atgccgcgtt
     2761 tgcataaatt tgtcacagat gcagaacctg ttgaaatgac cagttatatg gatgggcgga
     2821 aatatatcga tcaaaataat taccctgacc gtgttcgtat tggggcaggc aggcaatatt
     2881 ggcgatctga tgaagatgag cccaataacc gcgaaagttc atatcatatt gcaagtgcgt
     2941 attcttggct cgttggtggc aatacctttg cacaaaatgg atcaggtggt ggcacagtca
     3001 acttaggtag tgaaaaaatt aaacatagcc catatggttt tttaccaaca ggaggctcat
     3061 ttggcgacag tggctcacca atgtttatct atgatgccca aaagcaaaag tggttaatta
     3121 atggggtatt gcaaacgggc aacccctata taggaaaaag caatggcttc cagctggttc
     3181 gtaaagattg gttctatgat gaaatctttg ctggagatac ccattcagta ttctacgaac
     3241 cacgtcaaaa tgggaaatac tcttttaacg acgataataa tggcacagga aaaatcaatg
     3301 ccaaacatga acacaattct ctgcctaata gattaaaaac acgaaccgtt caattgttta
     3361 atgtttcttt atccgagaca gcaagagaac ctgtttatca tgctgcaggt ggtgtcaaca
     3421 gttatcgacc cagactgaat aatggagaaa atatttcctt tattgacgaa ggaaaaggcg
     3481 aattgatact taccagcaac atcaatcaag gtgctggagg attatatttc caaggagatt
     3541 ttacggtctc gcctgaaaat aacgaaactt ggcaaggcgc gggcgttcat atcagtgaag
     3601 acagtaccgt tacttggaaa gtaaacggcg tggcaaacga ccgcctgtcc aaaatcggca
     3661 aaggcacgct gcacgttcaa gccaaagggg aaaaccaagg ctcgatcagc gtgggcgacg
     3721 gtacagtcat tttggatcag caggcagacg ataaaggcaa aaaacaagcc tttagtgaaa
     3781 tcggcttggt cagcggcagg ggtacggtgc aactgaatgc cgataatcag ttcaaccccg
     3841 acaaactcta tttcggcttt cgcggcggac gtttggattt aaacgggcat tcgctttcgt
     3901 tccaccgtat tcaaaatacc gatgaagggg cgatgattgt caaccacaat caagacaaag
     3961 aatccaccgt taccattaca ggcaataaag atattgctac aaccggcaat aacaacagct
     4021 tggatagcaa aaaagaaatt gcctacaacg gttggtttgg cgagaaagat acgaccaaaa
     4081 cgaacgggcg gctcaacctt gtttaccagc ccgccgcaga agaccgcacc ctgctgcttt
     4141 ccggcggaac aaatttaaac ggcaacatca cgcaaacaaa cggcaaactg tttttcagcg
     4201 gcagaccaac accgcacgcc tacaatcatt taaacgacca ttggtcgcaa aaagagggca
     4261 ttcctcgcgg ggaaatcgtg tgggacaacg actggatcaa ccgcacattt aaagcggaaa
     4321 acttccaaat taaaggcgga caggcggtgg tttcccgcaa tgttgccaaa gtgaaaggcg
     4381 attggcattt gagcaatcac gcccaagcag tttttggtgt cgcaccgcat caaagccaca
     4441 caatctgtac acgttcggac tggacgggtc tgacaaattg tgtcgaaaaa accattaccg
     4501 acgataaagt gattgcttca ttgactaaga ccgacatcag cggcaatgtc gatcttgccg
     4561 atcacgctca tttaaatctc acagggcttg ccacactcaa cggcaatctt agtgcaaatg
     4621 gcgatacacg ttatacagtc agccacaacg ccacccaaaa cggcaacctt agcctcgtgg
     4681 gcaatgccca agcaacattt aatcaagcca cattaaacgg caacacatcg gcttcgggca
     4741 atgcttcatt taatctaagc gaccacgccg tacaaaacgg cagtctgacg ctttccggca
     4801 acgctaaggc aaacgtaagc cattccgcac tcaacggtaa tgtctcccta gccgataagg
     4861 cagtattcca ttttgaaagc agccgcttta ccggacaaat cagcggcggc aaggatacgg
     4921 cattacactt aaaagacagc gaatggacgc tgccgtcagg cacggaatta ggcaatttaa
     4981 accttgacaa cgccaccatt acactcaatt ccgcctatcg ccacgatgcg gcaggggcgc
     5041 aaaccggcag tgcgacagat gcgccgcgcc gccgttcgcg ccgttcgcgc cgttccctat
     5101 tatccgttac accgccaact tcggtagaat cccgtttcaa cacgctgacg gtaaacggca
     5161 aattgaacgg tcagggaaca ttccgcttta tgtcggaact cttcggctac cgcagcgaca
     5221 aattgaagct ggcggaaagt tccgaaggca cttacacctt ggcggtcaac aataccggca
     5281 acgaacctgc aagcctcgaa caattgacgg tagtggaagg aaaagacaac aaaccgctgt
     5341 ccgaaaacct taatttcacc ctgcaaaacg aacacgtcga tgccggcgcg tggcgttacc
     5401 aactcatccg caaagacggc gagttccgcc tgcataatcc ggtcaaagaa caagagcttt
     5461 ccgacaaact cggcaaggca gaagccaaaa aacaggcgga aaaagacaac gcgcaaagcc
     5521 ttgacgcgct gattgcggcc gggcgcgatg ccgtcgaaaa gacagaaagc gttgccgaac
     5581 cggcccggca ggcaggcggg gaaaatgtcg gcattatgca ggcggaggaa gagaaaaaac
     5641 gggtgcaggc ggataaagac accgccttgg cgaaacagcg cgaagcggaa acccggccgg
     5701 ctaccaccgc cttcccccgc gcccgccgcg cccgccggga tttgccgcaa ctgcaacccc
     5761 aaccgcagcc ccaaccgcag cgcgacctga tcagccgtta tgccaatagc ggtttgagtg
     5821 aattttccgc cacgctcaac agcgttttcg ccgtacagga cgaattagac cgcgtatttg
     5881 ccgaagaccg ccgcaacgcc gtttggacaa gcggcatccg ggacaccaaa cactaccgtt
     5941 cgcaagattt ccgcgcctac cgccaacaaa ccgacctgcg ccaaatcggt atgcagaaaa
     6001 acctcggcag cgggcgcgtc ggcatcctgt tttcgcacaa ccggaccgaa aacaccttcg
     6061 acgacggcat cggcaactcg gcacggcttg cccacggcgc cgttttcggg caatacggca
     6121 tcgacaggtt ctacatcggc atcagcgcgg gcgcgggttt tagcagcggc agcctttcag
     6181 acggcatcgg aggcaaaatc cgccgccgcg tgctgcatta cggcattcag gcacgatacc
     6241 gcgccggttt cggcggattc ggcatcgaac cgcacatcgg cgcaacgcgc tatttcgtcc
     6301 aaaaagcgga ttaccgctac gaaaacgtca atatcgccac ccccggcctt gcattcaacc
     6361 gctaccgcgc gggcattaag gcagattatt cattcaaacc ggcgcaacac atttccatca
     6421 cgccttattt gagcctgtcc tataccgatg ccgcttcggg caaagtccga acacgcgtca
     6481 ataccgccgt attggctcag gatttcggca aaacccgcag tgcggaatgg ggcgtaaacg
     6541 ccgaaatcaa aggtttcacg ctgtccctcc acgctgccgc cgccaaaggc ccgcaactgg
     6601 aagcgcaaca cagcgcgggc atcaaattag gctaccgctg gtaaccgccg gatatgccga
     6661 aaggggtctg acgatgccac cgtgcgctgt caaacccttt ttctgccgcc gccttgtgcc
     6721 tgctccattg tctgataacc gtcaaattga tttttagccc atgtttggtg ggtcggggaa
     6781 atctatatct tcgtccgtgc cgaaatagtc tgagaccttt gcaaaattcc tttccctccc
//
LOCUS       DAWIVB010000117         6791 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWIVB010000117 DAWIVB010000000
VERSION     DAWIVB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.117"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(84..1376)
                     /locus_tag="WIX60_001530"
     CDS             complement(84..1376)
                     /locus_tag="WIX60_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ5305783.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGKVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAEGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1454..2422
                     /locus_tag="WIX60_001531"
     CDS             1454..2422
                     /locus_tag="WIX60_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305784.1"
                     /translation="MKLTLAIPSLNLDEDEIRIPLCLHAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLVERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     ICRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDGTNGGAQGGTLFADTVWSRFH
                     SDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(2623..4089)
                     /locus_tag="WIX60_001532"
     CDS             complement(2623..4089)
                     /locus_tag="WIX60_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ5305785.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFIVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4331..6466)
                     /locus_tag="WIX60_001533"
                     /pseudo
     CDS             complement(4331..6466)
                     /locus_tag="WIX60_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1449 a         2016 c         1724 g         1602 t
ORIGIN      
        1 tttttgttaa tccactataa atgccgtctg aaaacctgtt tgcaggtttt cagacggcat
       61 tgttttcgtc gccggttcaa acttcaatcc gattgcgaca cggtaaccgg tatgccgcgc
      121 aagcgcgcaa gcagcgcgtc cgccttctgt ttctgcgcgg caaacgccgc cttgtccgcc
      181 tgcgtcaatt ccggtgtcgg caatgcgacc gaaacaggat tgacgggctg cccgttgatg
      241 cgcgcctcgt aatgcaggtg cggcccggtc gaacgcccgg tcgaaccgac aaaaccgatg
      301 acctcgccgc cgcgcacatt gccttccgcc tgcgaaaacg cgctcaagtg cgcgtacagc
      361 gtttccacac cgttggcgtg gcgtatcatc accgcgttgc cgtatccgcc cttccggcct
      421 ttaaaggtaa tcacgccgtc ggcggaagcc ctgaccggcg ttccctgcgg tgcggcataa
      481 tcgatgcccg tgtgcagccg ccatgtgtgc aggatggggt gcatacggta gccgaacggc
      541 gaagaaatgc gcgtatagac cagcggctcg atgttgaagc cgcctttttc ctgcaacacc
      601 ttgccgtctt catcataata attgccgccc cctccgcctt ccttgtccga acggtaatag
      661 aacgcctgat gccttgtgcc gcccttaacg acttcagccg ccaaaatatc gcccgccgcc
      721 acctgctgcc cgtggaaata caggctgtcg tacatcaggc gcacggcatc gccttccttc
      781 aaaccgtcaa ggctgaagcg gccggcgaaa atcccgctta aggattcgcg gatttcgacg
      841 ggcacttccg cccgcgccag cgaaccgcgc gccgacgttt tgaccacgac cgaacgcagc
      901 gtcggcaaaa ccttcatatc cgcctcagaa gccgaccgcc gccatatgcc gcctttcttt
      961 tccaaagcga ccagattgcg ctcgccgtct tcgtcggtaa aaaactgcac ttcgcgcgcg
     1021 ccgccgtcgc cgccgaccaa aacatgaacc gactggtcgg cacgcaaatg ccgcaaatcg
     1081 gcttcgccgc catatttttc cgtgattcgg gcaatctcgt cccgcgccat acccgaacgc
     1141 gccagcacgt ccgccagcga gtcgcccggc tgcaccgcct cctgcaccca atatgccgtc
     1201 tgaacgccgc tgccgcccca agacagcggc ggcagatttt gttccacgcg ctgcggtctg
     1261 acgcgctccg tcctctctgt cgaagcaatg tatgccgccg acaccaaaat aatcgaaacg
     1321 gcaagcgcac gcagcgcgta tttccgatgt tttgccgaaa gtgggaagac agccatattg
     1381 ttcctttttg acggcgtttg cgccgaatcc tctataatgt tgcgcctgac ctgcatttct
     1441 gaaacgtcaa acgatgaaac tcaccctcgc cattccctcg ctcaatttgg atgaagacga
     1501 aatccgaata cctctatgcc tgcacgcttt caacaaaatc ctgcaatacg gctcgctgca
     1561 ccgccaaagc tgcacggctt cggcatttta cgcgcgttat ttgtggtgcg gcagactggt
     1621 ggaacgcgcg gcgcaaagcc tgaatatgcc gtctgaagcc gtcgcgctcg ccacgcccgt
     1681 ctggcaaaag atggggatgc atcaggcaaa tgtgctaacg gcggaatatc tgaacgtcgg
     1741 tacggatgaa gcggaacgga tctgccgcga cctgtcggcg ttttacggcg acattccatg
     1801 gcgtttcgtt cccgtcctgc ccgaattatg gctggtttcc cttccttgcg cgtaccgctg
     1861 gggcgcgaag cccgttttgg acttgggcgg attgttgggc gctgacgatc agccggacgg
     1921 agaagatgcg ttggaatggc tgcgcgtcca aaccgaaatc caaatgtggc tggcggcgca
     1981 ccccgtcaac cataaccgca agaaacgcgg gctgcccgag ttgaacggct tatggctttg
     2041 ggacggcaca aacggcggcg cgcaaggcgg cacgcttttc gccgatacgg tttggagccg
     2101 tttccactcc gaccgccgcg cattacccga caacttccgc gcctatgaag aaacggcggc
     2161 acacctgccc gacacgcacc atatcctgtt tatggacgat ttgcgcctga ccgccctgac
     2221 aggggacagg gagcggtatg cggcaatatt gcagcagtgg gaagaacgct ggttcgcacc
     2281 aatctacgaa gccgtccgca ccggcaaaat caaacggctg gacatcacca cggacggaca
     2341 acacggcggt acgctgacct tcaaacccgc cgacaggtgg aaattttggc gcggcgcgaa
     2401 aacctttgac ggaatctggt gaacccgccc gccgcaaccg cgacaatgcc gtctgaagcc
     2461 cgaatcgggt ttcagacggc attttacctc gcatcgccgt tatgccggaa aaaccggaat
     2521 gcgtttccgc acacgcggcg gcacgcaaag caccgcccga aagccgccga tgcccgccgt
     2581 gataccgccg ccggcagggt ttcctccgaa aaggaacagc aatcagaacg ttttattaaa
     2641 ctcgacaaac gcccgatttt tctcgtattc gttgaacaca tcgttactcc gcgtttcgcg
     2701 gtgcgacaac gtcaggcgcg gcgtgatgcc tttgaaatgc aatgcccggt gccaaaggct
     2761 caaggatgtg ttcaattctt tatccctgcg cctttcccct ttaaaaccgc tgaaaaagcc
     2821 gggtttttca taatgccgtt tcgccgcgcc gaggcgcaac agcgaagaca ggccgctgcc
     2881 gccccattcc tgcccccagg caaagcgcag gccgtaacgg ttgaaattgt cgccccggtc
     2941 ggcggggttg cgctcgcggt aaaaatccaa accgcccatc caatattggc gcgcattccg
     3001 gtaaaacacc agcgaattgg aaatttgcaa atgggtattg tcggaacgcg cccggcgcgt
     3061 attcttcaaa cgcccccact ccgccgaaga caacgtttgc catttcgggg tttgccaacg
     3121 gttgaaataa aggcgtgcgc cgttggtgta agaataagcg tcgttgccgt aggtgcggcg
     3181 ttcgtggaac actgccagcc cggcatcttt gcgccggtcg gcaaaaccga tgccgccgga
     3241 aacgcctgcc gtcatatcgt tgaatttctt attccccgga taaaccctgc cggacacgtc
     3301 gccgcccgcc gtcgtgtacc agccgttttt cagcgaccat tttttctccg cgccgagccg
     3361 gtaattgacc gccgtgccgt ccacctgttt cgggaaagtc catttgccgt actgctgccg
     3421 tttcggggct tggttgatat tgtgttcgcg ggtgacgatg aagccgccat ttaccttcca
     3481 cgcatcgcgt tcgcgcaatg ccttgcggta cagctcgacc tgctccatca gctgcggcgg
     3541 caggttttcc gccttcaggc ggtcgaactg gtctgccgcc gcctcgttct gcctgttttc
     3601 aaacaatgct gccgccaaac gcatacggac ggcgggcgcg tcgggttggg cggcaatcaa
     3661 ttcccggtaa tgggaaatcg cctccttcac cctaccgtct gcctgcgcca aaatcccttg
     3721 tgcataaagt gccaacatct tatcctgctg cgcctgttgt aggtaaatcg gcaaaataac
     3781 gcggataccg gcaatattgt ttgagaccac tgcggaatac atcgcgcggg acaacaattc
     3841 gggatttttc agcagggttt cgccgtcaat ctgcaaaact tttccttttt cccgcacctg
     3901 ccccggcacc ttctccctgt cgatcggttt gacctccgct tcatgaagcc tgaactcggg
     3961 acggcttctc aaatccggtt cgcgcggtgt ttcttcggca tatgccgcgc ttgccaataa
     4021 aggcagcagc agcatccatc tgtttcgtgc aggcataaat gtcttcccac aaaaataaaa
     4081 aataaccatt atcaatcggc ggattgtatc agttttttat gccgctgttc caaaccctgc
     4141 cgcccgatgc cgcgacaatg ccgtctgaag ccagaatcgg gtttcaaacg gcggcaaaaa
     4201 accggcggtt ttccgccggt ttcatcatta tgccccgccc ttcggggcgg cagggttcgg
     4261 attggtttgc ggggcggcgc gcggtgccgt gccggtgtac cgcctgtgcg tcaaaacatt
     4321 tcccgacgca tcaaaacagc catttgagcg acaatgcggc ttctttgtcg ccgtccgtcc
     4381 ttttgccata tccgatgcgt gcggacatat ggcctttcca accggcttca ataccgaacc
     4441 gcccttcgag tgccgtcctg cctgccagcg tctgtttttc gccgtccatt tccacgccga
     4501 aagattttga cctgtgcaaa acattaaaag cggcaaaagg ctgaagattg acaccgttac
     4561 gcaaagcaaa acgggttttt gcccgaatgc cggcgcggct ttgccactga ccgctgccga
     4621 gcagtccgac cgccgtcccc tcgctgtcgg taaagccgcc gtttacgccc aagtaggtaa
     4681 actgcgcctg cggttgtagg taaaaccgca cattattgcc ttttccgaca atgccttccg
     4741 ccacaagcgc gttgtagccg ccttcgacag aagccgtcca acctttggtt ttgtagcgtt
     4801 ccgcacggtt ttcatcattg atgcggtgtt tgaaacgttg gtattgcaac cagccgtcca
     4861 aatacgcacc cgtttgttta tcgcgcaact gatgccacgc agcataaaca cccccgccat
     4921 aaccatacaa atcactgcct gccgcaccgc ctttgccgtt gactgatgcg tgctggccgg
     4981 ccctgccgcc catcacgccg attgccagtc ggctgccttc attttgccgt acaaacacct
     5041 cgccgccgat ttgcacgcct ttgcgccacc cgtccgcagc cgcgccgccc cgtatatttt
     5101 gatgcgaccg gccgccgatg aagcgcagcc acaatttttg acgggacgga tcggcggcat
     5161 acacgtcgtc gcccctgtcg gcggcacgca gcccaaacaa agtatttgcc gcataagcct
     5221 gttgggcata taaaaccgct tccgcctcgg ggatagccaa acgctgcacg cccaagcaaa
     5281 atccgccttc gcattgcttg agcaataatt cgtacagtcc ctgccggtaa ttttgtttaa
     5341 acgcaaacga accggaatcc gccgtttttg ccgcaatcag tttgcgcccg tcgagcgttt
     5401 tcagattttc cggccggcgt tccaaaacca cgccggattg tccgctgact ttgtcttcaa
     5461 aggtcagggc gggttcgtcc gactcccgca cgtcgtagcc gaacaggaaa tccgcgccgc
     5521 ccgcataatc cttatggacg gtcagttttt catctttcgt gttcaggcgg atgcgggcat
     5581 tatcggaagt ttccagcgta tggatgtcgg aatggcggcg cggttcccaa agggaatctt
     5641 tcagcgtcat cttggtcagt tccaacacct gtccggcgac gtgggatttg ttttcgattt
     5701 tgacgtgtaa ttttttattg aagatgttgt tgagactagt ctgtgatgta ttactatgag
     5761 gagtgagtac ggaatcttct accaatttat taattaaatc gggcatcgtc tgtttgagcg
     5821 tactaaattt tgttccaaac tgtgctatat acgcctcctc agtccgtttt ttattttctg
     5881 cccaagcttc cggtcttgtt ttgtataaat cctgtaattg acttttaacc gcctgcttta
     5941 aatcagcccc atacttctct gtaatatatt ttttgacaac gggcatatta tcgacggcga
     6001 ggatgccgct attgtaattt ttaccttgga tattgatttc gtcataccca tattcggatg
     6061 aattttgatc ggttttaccg ctggaaaaat aatgggaagt gccgggtttc ggcttgtctg
     6121 aaaatgaaaa atcggcatcg tcgccgattt tggtagaacg agtatttgaa ccaagctgag
     6181 ctctgaacat accgaagcaa tcaaaagata tgctgcacgg gccgcgccaa acgtgggaat
     6241 ttctgtcttc cgccgcttct aaaataaatt tgtttttaat ctcggcttct ttgacgggat
     6301 cgtacgcatc cgaacctcaa acatatggcg tgcaaaacag cgataaaaca gataatgtta
     6361 atagattaac ccgaccgctt gcttgcttgc ttgcttgctt gcttgcttgc ttgcttgctt
     6421 gcttgcttgc ttgcttgctt gcttgcttgc ttgctttcag tgtcatgaga aattttcctt
     6481 tgtcaagtgt taaaattata atgattatat actacattat actacatcgc aatgaaaaat
     6541 caggaaaagc aaaaaaccct ccgccgtcat tcccgcgaaa gcgggaatct agaattttaa
     6601 tgccgcaaga atttatcaag gatggtcggg gtttaaaggt ctggattccc gcctgcgcgg
     6661 gaatgacggt ttagaaatca cccgaaacaa gaaaaagaac cgaaaccgaa caagccggat
     6721 tcccgcctgc gcgggaatga cgggatttca ggttactgtt tttgattttc tgttttgtgg
     6781 aaataacggg a
//
LOCUS       DAWIVB010000118         6628 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWIVB010000118 DAWIVB010000000
VERSION     DAWIVB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.118"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="WIX60_001534"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="WIX60_001534"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ5305786.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1510
                     /locus_tag="WIX60_001535"
                     /pseudo
     CDS             1102..1510
                     /locus_tag="WIX60_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
     gene            1531..3594
                     /locus_tag="WIX60_001536"
     CDS             1531..3594
                     /locus_tag="WIX60_001536"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ5305787.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQIGKPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLVVLHGGDIVNVSVLGLQSGNKTLGHRFLSDGEITIENADSYAAQMREQGKVVA
                     SFAERKAAIQTVLEGQARRLNATAAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLTNVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEITEAVEQHYQPRFAGDNLPEGKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFN
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3611..4657
                     /locus_tag="WIX60_001537"
     CDS             3611..4657
                     /locus_tag="WIX60_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ5305788.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIAQRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     GGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHEHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4699..5526
                     /locus_tag="WIX60_001538"
     CDS             4699..5526
                     /locus_tag="WIX60_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ5305789.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDTAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5538..5633
                     /locus_tag="WIX60_001539"
     CDS             5538..5633
                     /locus_tag="WIX60_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ5305790.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            5675..6517
                     /locus_tag="WIX60_001540"
     CDS             5675..6517
                     /locus_tag="WIX60_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ5305791.1"
                     /translation="MQNHVISLASAAERRAHIAATFGVRGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1698 a         1779 c         1735 g         1416 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacatt ctgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgccggcac atcccatccc gccacctgcc
      241 tgcgcgcact cggccccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 ccctcaagcc cgctcccgcc aatatccaag acctctatct cgactctctg cgcgaattgg
      421 gcatcgatcc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggcgcgtg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcatcgact gcacgcccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacttgcaag gtgtagaaaa cgtctacgac ctcgtttggg
      661 caaaaacgct cgacggcaac accgtcacct acggcgacgt gtaccaccaa aacgaagtcg
      721 agcaatccac ctacaacttc gaatacagcg atgccgactg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccctgcctg
      841 cctacgagct tgtcctcaaa gcggggcata cgttcaacct tttagacgca cgcggcgcga
      901 tttccgtaac tgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaacgccg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccggaagcc
     1081 attatatttt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gaggacgatg ccgaagcgtt cgaccgcttt
     1261 atggacgagc tgattgcgta tcttgcggca aacggctttg aaatggcggg ggggcgattg
     1321 cggcgatttt tcgtgcctgt ttcaaaaatc cggtgcccga agcttgggag aaaccgagaa
     1381 aaaagccatt atctcttggc tggcagaccg cgaagatgtt gccactttgt acgccacccc
     1441 gctggttgac ggtttttatg gggatgacgg ctgggaaaat tacagcgaaa cctataaaca
     1501 ggcagattaa ccaaattcaa agaaagccga atgatgaccc aaaccctttt aatcgaactc
     1561 cttaccgaag aactcccgcc caaagccctg aataatctgg gcaaccattt cgccgcttcc
     1621 gttgccgaag gcttggaaaa agcgcaactg gttgacggcg cagccgaatt tacggcttat
     1681 gcctcgccgc gccgtttggc ggttcaagtc aaaaacgtca aagccgttca ggctgatcaa
     1741 aaaattgtga aaaaaggccc tgccgtagcg aatgccatga aagacggtgc gccaaccaag
     1801 gctttggaag gtttcgcacg cggtgcgggc gcgaaaatcg aagacttgac catcgtccac
     1861 gacggcaagc aggacgtgta cgcctacgaa tacgtccaaa tcggcaaacc gttgggcgga
     1921 cttttggaag acattatcaa tcaagcggtt aagaagctgc cgattcccaa agtaatgcgt
     1981 tggggcagca gcacgtttac ctttgtgcgc cccgttcacg ggctagttgt actgcacggc
     2041 ggcgacattg tgaacgtcag cgttttgggt ctgcaaagcg gcaacaaaac cttgggacac
     2101 cgcttcctct ccgacggcga aattaccatt gaaaacgccg acagctacgc cgcacaaatg
     2161 cgcgagcaag gcaaagtcgt cgcttcgttt gccgagcgca aagccgcgat tcagacggta
     2221 ttggaagggc aggcacgccg tctgaacgcg accgccgccg ccgatgaagc cttgttggac
     2281 gaagtaaccg cgctggtcga atggcctgtg gtattggaag ccggttttga agaacacttc
     2341 ctcgccgtac cgcaagaatg cctgattctg acgatgcagc aaaaccaaaa atacttcccg
     2401 ctgctcgacc aaaacggcaa gctgatgaac cgcttcctgc tggtgtccaa tctgcaaacc
     2461 gaagatccgt cacacatcat ccaaggcaat gaacgtgtct tgcgcgcgcg cctgtctgat
     2521 gccgagttct tctacaaaca agaccaaaaa gcgactttgg aaagccgcct gcccaaactg
     2581 acgaacgtgg tttatcacaa caaaatcggt tcgcaagccg aacgcatcga acgcctgcaa
     2641 agcatcgccg cccatatcgc caaggctttg ggcgcggatg ccgccgcagc cgaacgcgcc
     2701 gcgcgtttgg caaaagccga cttagtaacc gaaatggtcg gcgagttccc cgaactgcaa
     2761 ggcacgatgg gcaaatacta cgcccgcttg gacggcgaaa ccgaagaaat taccgaagcc
     2821 gtcgagcaac actaccaacc gcgttttgcc ggcgacaacc tgcctgaggg caaaattgcc
     2881 gccgccgttg cgcttgccga caaactggaa accttggtcg gcatttgggg tatcggcctg
     2941 attccgaccg gcgacaaaga cccatacgcc ctgcgccgcg ccgcattggg tattttgcgt
     3001 atgctgatgc agtacggttt ggacgtaaac gaactgattc agacggcctt caacagcttc
     3061 ccgcaaggtt tgctcaacga aaaaacgccg tctgaaaccg ccgacttcat gcaggcgcgc
     3121 cttgccgtat tgctgcaaaa cgattatccg caagacatcg ttgccgccgt acttgccaag
     3181 cagccgcgcc gtttggacga tttgaccgcc aaactgcagg ctgtcgccgc gttcaaacaa
     3241 ctgcccgaag ccgccgcgct cgccgctgcc aacaaacgcg tgcaaaacct gctgaaaaaa
     3301 gccgatgccg agttgggcgc ggttaacgaa agcttgctgc aacaggacga agaaaaagcc
     3361 ctctttgccg ccgcacaagg cttgcagccg aaaattgccg ccgccgttgc cgaaggcaat
     3421 ttccagaccg ccttgtccga actggcttcc gtcaaaccgc aagtcgatgc cttcttcgac
     3481 ggcgtgatgg tgatggcgga agatgccgcc gtgaaacaaa accgcctgaa cctgctgaac
     3541 cgcttggcag aacaaatgaa cgcggtagcg gatatcgcgc ttttgggcga gtaaccgttg
     3601 tacagtccaa atgccgtctg aagccttcag acggcatcgt gcctatcggg agaataaatt
     3661 gcagccttta gtcagcgtat tgatttgcgc ctacaacgta gaaaaatatt tcgcccaatc
     3721 attagccgcc gtcgtgaatc aaacttggcg caacttggat attttgattg tcgatgacgg
     3781 ctcgacggac ggtacgcttg ccattgccca gcgtttccaa gaacaggacg gccgcatccg
     3841 catcctcgcg cagccgcgca attccggtct gattccctct ttaaacatcg ggctggatga
     3901 attggcaaag tcgggggggg gggaatatat tgcgcgcacc gatgcagacg atattgccgc
     3961 ccccgactgg attgagaaaa tcgtgggtga gatggaaaaa gaccgcagca tcatcgcgat
     4021 gggtgcgtgg ctggaagttt tgtcggaaga aaaggacggc aaccggctgg cgcggcacca
     4081 cgaacacggc aaaatttgga aaaaaccgac ccgacacgaa gacattgccg actttttccc
     4141 tttcggcaac cccatacaca acaacacgat gattatgagg cgcagcgtca ttgacggcgg
     4201 tttgcgttac aacaccgagc gggattgggc ggaagattac caattttggt acgatgtcag
     4261 caaattgggc aggctggctt attatcccga agccttggtc aaataccgcc ttcacgccaa
     4321 tcaggtttca tccaaataca gcatccgcca acacgaaatc gcgcaaggca tccaaaaaac
     4381 cgccagaaac gattttttgc agtctatggg ttttaaaacc cggttcgaca gccttgaata
     4441 ccgccaaata aaagcagtag cgtatgaatt gctggagaaa catttgccgg aagaagattt
     4501 tgaacgcgcc cgccggtttt tgtaccaatg cttcaaacgg acggacacgc tgcccgccgg
     4561 cgcgtggctg gattttgcgg cagacggcag gatgcggcgg ctgtttacct tgaggcaata
     4621 cttcggcatt ttgcaccgat tgctgaaaaa ccgttgaaaa acgccgcttt atccaacaga
     4681 caaaaaacag gataaattat gcaaaaccac gttatcagct tagcttccgc cgcagaacgc
     4741 agggcgcaca ttgccgatac cttcggcagg cacggcatcc cgtttcagtt tttcgacgca
     4801 ctgatgccgt ctgaaaggct ggaacaggca atggcggaac tcgtccccgg cttgtcggcg
     4861 cacccctatt tgagcggagt ggaaaaagcc tgctttatga gccacgccgt attgtggaag
     4921 caggcattgg acgaaggtct gccgtatatc accgtatttg aggacgacgt tttactcggc
     4981 gaaggtgcgg aaaaattcct tgccgaagac gcttggctgc aagaacgctt tgacccggat
     5041 accgccttta tcgtccgctt ggaaacgatg tttatgcacg tcctgacctc gccctccggc
     5101 gtggcggatt actgcgggcg cgcctttccg ctgttggaaa gcgaacactg ggggacggcg
     5161 ggctatatca tttcccgaaa agcgatgcgg tttttcctgg acaggtttgc cgccctgccg
     5221 cccgaagggc tgcaccccgt cgatctgatg atgttcagcg attttttcga cagggaagga
     5281 atgccggttt gccagctcaa tcccgccttg tgcgcccaag agctgcatta tgccaagttt
     5341 cacgaccaaa acagcgcatt gggcagcctg atcgaacacg accgcctcct gaaccgcaaa
     5401 cagcaaaggc gcgattcccc cgccaacaca ttcaaacacc gcctgatccg cgccttgacc
     5461 aaaatcagca gggaaaggga aaaacgccgg caaaggcgcg aacagttcat tgtgcctttc
     5521 caataaaagg aggagagatg gacatcgtat ttgcagcaga cggcaggatg cggcggctgt
     5581 ttaccttgag gcaatacttc ggcattttgc gccgattgct gaaaaaccgt tgaaaaacgc
     5641 cgctttatcc aacagacaaa aaacaggata aattatgcaa aaccatgtta tcagcttagc
     5701 ttccgccgca gaacgcaggg cgcacattgc cgcaaccttc ggcgtgcgcg gcatcccgtt
     5761 tcagtttttc gacgcactga tgccgtctga agaactgaat cggatgatgg cggaattggt
     5821 gccgggattg gcaaaacagc acctgttgag cgaagtggaa aaagcctgct ttatgagcca
     5881 cgccgtattg tggaaacagg cattggatga aggtctgccc tatgttgccg tatttgagga
     5941 tgatgtcctg cttggcaaag acgcagaaaa gttccttgcc gaagatactt ggttggaaga
     6001 gcgttttgat aaggattctg cctttatcgt tcgtttggaa acgatgtttg cgaaagttat
     6061 tgtcagaccg gataaagtcc tgaattatga gaatcggtca tttcctttgc tggagagcga
     6121 acattggggg acggctggct atatcatttc gcgtgaggcg atgcggtttt tcttggaaag
     6181 gtttgccgtt ttgccggcag agtggattaa agcggtagat tggatgatgt ttacttattt
     6241 ctttgataag gaggggatgc ctgtttatca ggttaatccc gccttatgta cccaagaatt
     6301 gcattatgcc aagttcctca gtaaaaacag tatgttgggt agcgatttgg aaaaagatag
     6361 ggaacaagaa agaagacacc gccgttcgtt gaaggtgatg tttgacttga agcgtgcttt
     6421 gggtaaattc ggtagggaaa agaagaaaag aatggagcgt caaaggcagg cggagcttga
     6481 gaaagcttac ggcaggcggg tcatatcgtt caaatagttt gtgtaaaata tagcggatta
     6541 aaattagaaa tggatacact gtcattcccg cgcaggcggg aatctagtcc gttcggtttc
     6601 gcttgtttta agtttcgggt aacttcca
//
LOCUS       DAWIVB010000119         6465 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWIVB010000119 DAWIVB010000000
VERSION     DAWIVB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.119"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(90..1886)
                     /locus_tag="WIX60_001541"
     CDS             complement(90..1886)
                     /locus_tag="WIX60_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ5305792.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIVLQKSGQVPPYNEWLAAS
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIEHPDNLLNQVWTNRPALPAETVFIH
                     DPDYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGIAVEPYAQVADKLAQIGGVLLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPSAEQKSDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            1942..2283
                     /locus_tag="WIX60_001542"
     CDS             1942..2283
                     /locus_tag="WIX60_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305793.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFMPFFGSNMENIVKFEHEVLIVCVGRYLDYVTIRVFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2344..3597
                     /locus_tag="WIX60_001543"
     CDS             2344..3597
                     /locus_tag="WIX60_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ5305794.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3752..5263
                     /gene="lysS"
                     /locus_tag="WIX60_001544"
     CDS             3752..5263
                     /gene="lysS"
                     /locus_tag="WIX60_001544"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ5305795.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVVQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5340..5528)
                     /locus_tag="WIX60_001545"
     CDS             complement(5340..5528)
                     /locus_tag="WIX60_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ5305796.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLETE"
     gene            complement(5532..6409)
                     /locus_tag="WIX60_001546"
                     /pseudo
     CDS             complement(5532..6409)
                     /locus_tag="WIX60_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
BASE COUNT         1403 a         1671 c         1853 g         1538 t
ORIGIN      
        1 cttgcggcaa gatgaaaacg gtgcgggatg ttttgggaaa ccaatgccgt ctgaagggct
       61 ttcagacggc attttttgcg ccgtgctgtt taacgcgcca gcggttcggt gcgtttgatc
      121 agccacgctt ttgccgcgcc ttcggtcagc ggctcgaggc ggcggcggac ttcggcgtgg
      181 tagcggttga cccagtcgat ttcgccgtcg gtcatgaggg cggtgtccat caggcgggtg
      241 tcgatggggc agagggtcag ggtttcaaaa cagaggaagc tgccgaattc ggtttcttga
      301 ggggcggcga cggcttggtt ggcggcaagg ttttcaatgc ggatgcccca ttttcccggg
      361 cggtagagtc cgggttcgat ggaggtaacc atgccttttt tcatggcggt ttcgggcgtg
      421 gcgggggcgg cgaaggcgat gcgctgcggg ccttcgtgga cgttgaggaa atagcctacg
      481 ccgtgtccgg tgccgtggcc gtagtcgcat tgcgcctgcc acaggggttt gcggcaaatc
      541 gcatcaatca gcggcgaggg gatgttttcg gggaacacgg cttcggcaag cgcgatatgg
      601 gctttgagaa cgagggtgtt gtcgcttttt tgttcggcac tcggcgtgcc gacggggacg
      661 acgcgggtga tgtcggtcgt gccgcctttg tattgcgcgc cggagtcgat gagcaaaagc
      721 ccgttgccgc tgatggtgct gtggctttcg ggtgtcgcgc tgtaatgcgg cagtgcgccg
      781 ttggcgttga agcctgcgat ggtgtcgaaa ctcaatgaaa tgaagcctgg gcgcacgctg
      841 cggtggcgat aaagcatggt gtccacgtcg atttcggtca ggctgccgcc gttgccgatg
      901 atgtcttcaa actcggcgaa gaaaccgcac aacgccgcgc cgtcgtgttc catcgcttcg
      961 cggatgcggg cgatgtcggc ttcggatttg caggatttga acagcgtgga tgggttgatt
     1021 ccctcgataa ggcgcacgct ttcgggcagg cgcacaagcg tgctgacggc ggttttgttc
     1081 ggctcgatga gcagcacgcc gccgatttgc gcgagtttgt cggcaacttg ggcgtaaggt
     1141 tcgaccgcga tgccggcggt ttgcagcgcg gcggcggctt cggcgttcag acggcatcgg
     1201 tcggtaaaca ggacggcgtt gtctttgcca atcagcagga aggacacgaa aacgggattg
     1261 aaaggcacgt cgctgccgcg caggttggtc agccaggcga tgtcgtcaag cgaggaaacc
     1321 aagtggtaat ccgcgccttt ttccgccatc acggcgcgca cgcgggcgag tttttcggcg
     1381 gcggtttcag aaacatagtc ggggtcgtgg atgaacaccg tttcggcggg gagggcgggg
     1441 cggtttgtcc acacttgatt cagtaaatta tccgggtgtt cgatgcggat gtttttggcg
     1501 gcgagtgatt gcgccaaagt gcgtttgccg gtgagcgaga ccatatcgga agggatgccg
     1561 acggcggcgt tttcgggcag gcttgccgcg agccattcgt tgtacggcgg cacttgcccg
     1621 cttttttgca gcacaatgcc gctgcccgca agctgtttgg cggcttgttc ccaatagcgg
     1681 ctgtccaccc acacgcccgc ttcatcggtg gtcaggacaa acgtgccgac cgagccggta
     1741 aagcccgata attcgcggcg cgcctgccaa tgctcgggca ggtattcgga caggtggggg
     1801 tcggcggaag ggatgacgag tgcatccaag ccttgcgcct tcatggcttc gcgtaatgcg
     1861 gacagataat tcgatacggt attcataaca gttcctccaa tcgggttttg cggcttcaga
     1921 tggcatggac ggaaatctga aatgccgtct gaaaaacata accatcttat caaatcgcca
     1981 gcctgctgca aaacggacgg gctgatcgga tggtgcgtgt ttgcgcgaaa aaagcgatgt
     2041 aaggttgttt gttgtaaaat tttaggcata aaatgtcgtt ttatgccttt ttttggaagt
     2101 aatatggaaa atatagtcaa gtttgagcat gaagttctaa tagtgtgtgt tggcaggtat
     2161 ttggattacg ttacaatacg tgttttcatc aaaatttccc taccgggcgg tatcctgttt
     2221 ttgaagacgg taggatttgg gtttgtttgg gggcaatccc gcccaaattg gagcaataga
     2281 tagggctgtg tccgaatatg cggctctgtg tgttgaaaca cagttaaaaa tacggaagac
     2341 tttatgtccg aacaacatat ttcgacttgg aaaagtaaaa tcaacgcatt gggtccgggg
     2401 atcatgatgg cttcggcggc ggtcggcggt tcgcacctga ttgcctcgac gcaggcgggc
     2461 gcgctttacg gctggcagat cgcgctcatc atcatcctga ccaacctctt caaatacccg
     2521 tttttccgct tcagcgcgca ttacacgctg gacacgggca agagcctgat tgaaggttat
     2581 gccgagaaaa gccgcgttta tttgtgggta ttcctgattt tgtgcatcct ctccgccacg
     2641 attaacgcgg gcgcggtcgc cattgtaacc gccgccatcg tcaaaatggc gattccctcg
     2701 ctgatgtttg atgccggcac ggttgccgcc ttgattatgg catcctgcct gattattttg
     2761 gtgagcggac gttaccgcgc tttggatcgc gtttccaaaa tcatcatcgt tactttgagt
     2821 atcgccacgc ttgccgccgc cggcatcgct atgtcgcgcg gtatgcagat gcagtccgat
     2881 tttatcgagc cgacaccgtg gacgcttgcc ggtttgggct tcctgatcgc gctgatgggc
     2941 tggatgcccg cgccgattga aatttccgcc atcaattctt tgtgggtaac cgaaaaacaa
     3001 cgcatcaatc cttccgaata ccgcgacggg atttttgatt tcaacgtcgg ttatatcgcc
     3061 agtgcggttt tggctttggt tttccttgca ctgggcgcgt ttgtgcaata cggcaacggc
     3121 gaagcagtgc agatggcggg cggcaaatat atcgggcaat tgatcaatat gtacgccgtt
     3181 accatcggcg gctggtcgcg cccgctggtg gcgtttatcg cgtttgcctg tatgtacggc
     3241 acgacgatta ccgtcgtgga cggctatgcc cgtgccattg ccgaacccgt gcgcctgctg
     3301 cgcggaaaag acaaaacggg caacgccgaa ttctttgcct ggaatatttg ggtggcgggc
     3361 agcggtttgg cggtgatttt ctggtttgac ggcgtaatgg cgaatctgct caaatttgcg
     3421 atgattgccg cttttgtgtc cgcccctgtg tttgcctggc tgaattaccg tttggttaaa
     3481 ggtgatgaaa aacacaaact cacatcaggt atgaatgccc ttgcattggc aggcttgatt
     3541 tatctgaccg gttttaccgt tttgttctta ttgaatttgg cgggaatgtt caaatgataa
     3601 cgatgccgtc tgaaaccgca aaccgctttc agacggcatt gtcgcgttta tggaaaaaaa
     3661 tgccgacatc gcgtaaaata tgcgcaaatt ttgtgattcg gtcaggtcgt ctgaaacaga
     3721 ttgcgcctga tttatttttt cggaaaacct tatgagcgaa caaaaccatc cgcaaaccga
     3781 gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactgc acaacatccg
     3841 ccaacagcgc aacgcctatc ccaacgactt caaacgcgac agcttcgccg ccgatttgca
     3901 cgcccaatac ggcgaaatcg gcaaagaaga actcgatccg caaggcattc ccgtcaaagt
     3961 ggccggccgc atgatgctga agcgtcaaat gggcaaggcg agttttgcca ccattcaaga
     4021 cgtgtccggg caaatccagc tttatctgaa caacaaaggc gtgagccaag aagttttgga
     4081 cgacttcaac cattgggatt tgggcgacat cgtcggcgcg gaaggcactt tgttcaaaac
     4141 caaccacggc gaactgaccg tacgcgtgtc cggcatccgc ctgctgtcca aatccctacg
     4201 cccgctgccc gacaaacaca aaggtttgag cgatcaggaa accaaatacc gccaacgcta
     4261 tgttgatttg attgccaatg aagaatcgcg caataccttt atcaaacgca gccaaatcat
     4321 ccaatccgtg cgtaatttta tggtgggcga gcattatctc gaagtcgaaa ccccgatgat
     4381 gcacccgatt cccggcggcg cgacggcaaa acccttcgtt acccatcaca atgccttaga
     4441 tattccgctt tacctgcgta tcgcgcctga gctgtatttg aaacgcctgg ttgtcggtgg
     4501 tttggaacgc gtgtttgaaa tcaaccgcag cttccgcaac gaaggcatgt ccgtgcgcca
     4561 caaccccgaa ttcaccatga tcgaattcta cgaagccttc tccgactacg aacgcatgat
     4621 gcagatggcg gaagacatca tccgcaacgc atcgcgcacg gtaaacggca cggcaaacat
     4681 cacttacaac ggcaaagaag tcgatttgga aagcccgttt gaacgcctga ccattctcga
     4741 agccatcaaa aaatacaatc cgcactacac cgacgagcag ttgaacgatg cggaatggct
     4801 gaaaaaagaa atcgtcaaac acggcgaaag cctgccgccg tccccgggca tcggcagcct
     4861 gcaactcgcg ctgtttgaag gttgcgccga gggcaagctg tggaatccga ccttcatcgt
     4921 cgattacccg gtcgaagttt caccgttggc gcgcgcttcg gataccaaac aaggtctgac
     4981 cgaacgtttc gaattgttcg ttgtcggccg cgaactggca aacggctatt ccgagttgaa
     5041 cgaccccgaa gaccaagccg aacgcttcaa agcgcaagtg gtgcaaaaag acgcgggcga
     5101 cgacgaagcc atgcactacg atgccgacta catccgcgcg atggaattcg gtttgccgcc
     5161 gaccggcggt tgcggcatcg gtatcgaccg cttggtaatg ctgctgaccg attcgcaaac
     5221 catccgcgat gtgattctgt ttccgcagat gcgccccgag taatcataaa aacagttgaa
     5281 atggcaatgc cgtctgaacc cgattggatt cagacggcat tttgtatggc ggtacggatt
     5341 tattcggttt ccaactgacc gacccattcg gacaaggcag tcaggcgttg ttcggatttc
     5401 gccacgaacg ggttttcggt atcccacgcg tagcctgcca aaatcgaaga aagcatacgg
     5461 ctgatttccg ggctgcggtc gggcgcgtac acgacgtttt ggaagcggaa atcataccaa
     5521 ccgtccacac atcaggggcg gtaatccgaa tcaacacgcc ttcttgcctg tgcggcggac
     5581 ataaaccgtt ttttgactcc agcaccacac ctaaaatcgt gctgccttcg ggcaggtcaa
     5641 aggcttcaga cggcctgccc gtctgataaa tcagcatcct gcccgcaata tcgataacaa
     5701 ggtgcgcgac gaccagccgc ttgtcggaca gttcaatcag cgcgatgtct tcgtccgcag
     5761 ggcacatacg cggctcgacc accagcaagt ctcccttttc gataaccggc gacatggacg
     5821 tgtcgggcat ggcaacggca aacgtgtact gcgagggggc gaccaccgaa gcaaacaggt
     5881 cgaaactgcc cgtgagtttg cctgtacggg cgaaagccgc cgcctgacgg tctgacaaca
     5941 aaggacgggg tgtgagcctc atcgacagtg ctttgccatc ggcatcgctg tggttgtgcg
     6001 tatgcgtatg atggttgttg atggtcgtat cgccttggac atcgttaagg actatgctgt
     6061 ttgaacctgc cacggatttg gtcatctcat tgtaggcttg cgtcgtgatg cgctcttgct
     6121 tggtcatacc tgttacagcc tgtttcagca tttctaacgc tatgtgattc ttcctaaggg
     6181 tcgctatacc ggcttttgca gcttgctcat taccacaaga ggggcaaccc cagctacttc
     6241 ctatgaacat attggagtac ctcgaacagg tttggtttcc atgtttaggg cagttgatta
     6301 cacacggctt ggctgtaccc tcaaactcga ccaacttaat gccacgatga ccgaaacgtt
     6361 cctcaacctc agcagcagct tcttcaaatg tttttgacct tcttgccata ttgacctccg
     6421 gattattgat tattttggct atgctttaac gagggattgg aatga
//
LOCUS       DAWIVB010000120         6457 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWIVB010000120 DAWIVB010000000
VERSION     DAWIVB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.120"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            284..2131
                     /locus_tag="WIX60_001547"
     CDS             284..2131
                     /locus_tag="WIX60_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ5305797.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVLIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTQAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGMPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGVWYVR
                     DRAGIDARLGAEFLAEGRKIPGSAVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            2186..6355
                     /locus_tag="WIX60_001548"
     CDS             2186..6355
                     /locus_tag="WIX60_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ5305798.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
BASE COUNT         1509 a         2074 c         1704 g         1170 t
ORIGIN      
        1 ttgcgttgat ttgccctatg cagtagtgcc ggacaggctt tgctttatca ttcggcgcga
       61 cggtttaatt tattgaacga aaataaattt atttaatcct gcctattttc cgacactatt
      121 ccgaaacgca gcctgttttc catatgcgga ttagaaacaa aataccttaa aacaagcaga
      181 tacatttccg gcgggccgca acctccgaaa taccggcggc agtatgccgt ctgaagcgtc
      241 ccgccccgtc cgaacagtgt taaaatcgaa agccgccaca ccgatgcacg acacccgtac
      301 catgatgatc aaaccgaccg ccctgctcct gccggcttta tttttctttc cgcacgcata
      361 cgcgcctgcc gccgaccttt ccgaaaacaa ggcggcgggt ttcgcattgt tcaaaaacaa
      421 aagccccgac accgaatcag tcaaattaaa acccaaattc cccgtcctca tcgacacgca
      481 ggacagtgaa atcaaagata tggtcgaaga acacctgccg ctcatcacgc agcagcagga
      541 agaagtattg gacaaggaac agacgggctt cctcgccgaa gaagcgccgg acaacgttaa
      601 aacgatgctc cgcagcaaag gctatttcag cagcaaagtc agcctgacgg aaaaagacgg
      661 agcttatacg gtacacatca caccgggccc gcgcaccaaa atcgccaacg tcggcgtcgc
      721 catcctcggc gacatccttt cagacggcaa cctcgccgaa tactaccgca acgcgctgga
      781 aaactggcag cagccggtag gcagcgattt cgatcaggac agttgggaaa acagcaaaac
      841 ttccgtcctc ggcgcggtaa cgcgcaaagc ctacccgctt gccaagctcg gcaatacgca
      901 ggcggccgtc aaccccgata ccgccaccgc cgatttgaac gtcgtcgtgg acagcggccg
      961 ccccatcgcc ttcggcgact ttgaaatcac cggcacacag cgttaccccg aacaaatcgt
     1021 ctccggcctt gcgcgtttcc agcccggtat gccgtacgac ctcgacctgc tgctcgactt
     1081 ccaacaggcg ctcgaacaaa acgggcatta ttccggcgcg tccgtacaag ccgacttcga
     1141 ccgcctccaa ggcgaccgcg tccccgtcaa agtcagcgta accgaggtca aacgccacaa
     1201 actcgaaacc ggcatccgcc tcgattcgga atacggtttg ggcggcaaaa tcgcctacga
     1261 ctattacaac ctcttcaaca aaggctatat cggttcggtc gtctgggata tggacaaata
     1321 cgaaaccacg cttgccgccg gcatcagcca gccgcgcaac tatcggggca actactggac
     1381 aagcaacgtt tcctacaacc gttcgaccac ccaaaacctc gaaaaacgcg ccttctccgg
     1441 cggcgtctgg tatgtgcgcg accgcgcggg catcgatgcc aggctggggg cggaatttct
     1501 cgcagaaggc cggaaaatcc ccggctcggc tgtcgatttg ggcaacagcc acgccacgat
     1561 gctgaccgcc tcttggaaac gccagctgct caacaacgtg ctgcatcccg aaaacggcca
     1621 ttacctcgac ggcaaaatcg gtacgacttt gggcacattc ctgtcctcca ccgcgctgat
     1681 ccgcacctct gcccgtgcag gttatttctt cacgcccgaa aacaaaaaac tcggcacgtt
     1741 catcatacgc ggacaagcgg gttacaccgt tgcccgcgac aatgccgacg ttccttcagg
     1801 gctgatgttc cgcagcggcg gcgcgtcttc cgtgcgcggt tacgaactcg acagcatcgg
     1861 acttgccggc ccgaacggat cggtcctgcc cgaacgcgcc ctcctggtgg gcagcctgga
     1921 ataccaactg ccgtttacgc gcaccctttc cggcgcggtg ttccacgata tgggcgatgc
     1981 cgccgccaat ttcaaacgta tgaagctgaa acacggttcg ggactgggcg tgcgctggtt
     2041 cagcccgctt gcgccgtttt ccttcgacat cgcctacggg cacagcgata agaaaatccg
     2101 ctggcacatc agcttgggaa cgcgcttcta aaccgatatg gccacttcag acggcattgc
     2161 agcaaaccat tttgaaacag acattatgac cgataccgca ccgacagata ccgatccgac
     2221 cgaaaacggc acgcgcaaaa tgccgtctga acaccgccct accccgccgg caaaaaaacg
     2281 ccgcccgttg ctgaagctgt cggcggcact gctgtctgtc ctgattttgg cagtatgttt
     2341 cctcggctgg ctcgccggta cggaagcagg tttgcgcttc gggctgtacc aaatcccgtc
     2401 ttggttcggc gtaaacattt cctcccaaaa cctcaaaggc acgctgctcg acggcttcga
     2461 cggcgacaac tggtcgatag aaaccgaggg ggcagacctt aaaatcagcc gcttccgctt
     2521 cgcgtggaaa ccgtccgaac tgatgcgccg cagcctgcac attaccgaaa tttccgccgg
     2581 cgacatcgcc atcgttacca aaccgactcc gcctaaagaa gaacgcccgc cgctcagcct
     2641 tcccgacagc atagacctgc ctgccgccgt ctatctcgac cgcttcgaga cgggcaaaat
     2701 cagcatgggc aaagcctttg acaaacaaac cgtctatctc gaacggctgg atgcttcata
     2761 ccgttacgac cgcaaaggac accgccttga cctgaaggcc gccgacacgc cgtggagcag
     2821 ttcgtcgggg gcggcctcgg tcggcttgaa aaaaccgttt gccctcgata ccgccattta
     2881 caccaaaggc ggactcgaag gcaaaaccat acacagtacg gctcggctga gcggcagcct
     2941 gaaggatgtg cgcgccgaac tggcgatcga cggcggcaat atccgcctct cgggaaaatc
     3001 cgtcatccac ccgtttgccg aatcattgga taaaacattg gaagaagtac tggtcaaagg
     3061 gttcaacatc aatccggccg ccttcgtgcc ttccctgccc gatgccggac tgaatttcga
     3121 cctgaccgcc atcccgtcgt tttcagacgg catcgcgctg gaaggttcgc tcgatttgga
     3181 aaacaccaaa gccggctttg ccgaccgcaa cggcatcccc gtccgtcagg ttttaggcgg
     3241 ctttgtcatc cggcaggacg gcacggtgca tatcggcaat acgtccgccg ccctgctcgg
     3301 acggggcggc atcaggctgt cgggcaaaat cgacaccgaa aaagacatcc tcgatttaaa
     3361 tataggcatc aactccgtcg gcgcggaaga cgtactgcaa accgcgttca aaggcaggtt
     3421 ggacggcagc atcggcatcg gtggcacgac cgcctcgccc aaaatctctt ggcaactcgg
     3481 catcggcacg gcgcgcacgg acggcagcct cgccattgca agcgacccag caaacggaca
     3541 gcggaaactg gtgctcgaca ccgtcaacat cgccgccggg caaggcagcc tgaccgcgca
     3601 aggctatctc gagctgttta aagaccgcct gctcaagctg gacatccgtt cccgcgcatt
     3661 cgacccttcg cgcatcgatc cgcaacttcc ggcaggcaat atcaacggct caataaacct
     3721 tgccggcgaa ctggcaaaag agaaattcac aggcaaaatg cggtttttac ccggcacgtt
     3781 caacggcgta ccgattgccg gcagtgccga cattgtttac gagtcccgcc accttccgcg
     3841 tgccgccgtc gatttgcggc tggggcggaa cattattaaa acagacggcg gcttcggcaa
     3901 aaaaggcgac cggcttaacc tcaatatcac cgcacccgat ttatcccgtt tcggtttcgg
     3961 actcgcgggg tctttaaatg tacgcggaca cctttccggt gatttggacg gcggcatccg
     4021 aacctttgaa accgaccttt ccggcgcggc gcgcaacctg cacatcggca aggcggcaga
     4081 catccgttcg ctcgatttca cgctcaaagg ttcgcccgac acaagccgcc cgatacgcgc
     4141 cgacatcaaa ggcagccgcc tttcgctgtc gggcggagcg gcggttgtcg ataccgccga
     4201 cctgatgctg gacggcacgg gcgtgcagca ccgcatccgc acacacgccg ccatgacgct
     4261 ggatggcaaa ccgttcaaat tcgatttgga cgcttcaggc ggcatcaaca gggaacttac
     4321 ccgatggaaa ggcagcatcg gcatcctcga catcggcggc gcattcaacc tcaagctgca
     4381 aaaccgtatg acgctcgaag ccggtgcgga acgcgtggcg gcaagtgcgg caaattggca
     4441 ggcaatgggc ggcagcctca acctgcaaca cttttcttgg gataaaaaaa ccggcatatc
     4501 ggcaaaaggc ggcgcacacg gtctgcatat cgccgagttg cacaatttct tcaaaccgcc
     4561 cttcgaacac aatctggttt taaacggcga ctgggatgtc gcctacgggc gcaacgcgcg
     4621 cggctacctc aatatcagcc ggcaaagcgg cgatgccgta ttgcccggcg ggcaggcttt
     4681 gggtttgaac gcattttccc tgaaaacgcg ctttcaaaac gaccgcatcg gaatcctgct
     4741 tgacggcggc gcgcgtttcg ggcggattaa cgccgatttg ggcatcgcca acgccttcgg
     4801 cggcaatatg gcaaatgcac cgctcggcgg caggattacc gcctcccttc ccgacttggg
     4861 cgcattgaag ccctttctgc ccgccgccgc gcaaaacatt accggcagcc tgaatgccgc
     4921 cgcgcaaatc ggcggacggg taggctctcc gtccgtcaat gccgccgtca acggcagcag
     4981 caactacggg aaaatcaacg gcaacatcac cgtcgggcaa agccgctctt tcgataccgc
     5041 gcctttgggc ggcaggctca acctgaccgt tgccgatgcc gaagtattcc gcaacttcct
     5101 accggtcgga caaaccgtca aaggcagcct gaatgccgcc gtaaccctcg gcggcagcat
     5161 cgccgatccg cacttgggcg gcagcatcaa cggcgacaaa ctctattacc gcaaccaaac
     5221 ccaaggcatc atcttggaca acggctcgct gcgttcgcat atcgcgggca ggaaatgggt
     5281 aatcgacagc ctgaaattcc ggcacgaagg gacggcggaa ctctccggta cggtcggtat
     5341 ggaaaacagc ggacccgatg tcgatatcgg cgcggtgttc gacaaatacc gcatcctgtc
     5401 ccgccccaac cgccgcctga cggtttccgg caacacccgc ctgcgctatt cgccgcaaaa
     5461 aggcatatcc gttaccggga tgattaaaac ggatcagggg ctgttcggtt cgcaaaaatc
     5521 ctcgatgccg tccgtcggcg acgatgtcgt cgtattaggc gaagtcaaaa aagaggcggc
     5581 ggcaccgctc cccgtcaata tgaacctgac tttagacctc aatgacggca tccgcttcgc
     5641 cggctacggc gcggacgtta ccataggcgg caaactgacc ctgaccgccc aatcgggcgg
     5701 aagcgtacgg ggcgtgggca cggtccgcgt catcaaaggg cgttataagg catacgggca
     5761 ggatttggac attaccaaag gcacggtctc ctttgtcggc ccgctcaacg atcccaacct
     5821 caacatccgc gccgaacgcc gcctttcccc cgtcggtgcg ggcgtggaaa tattgggcag
     5881 cctcaacagc ccgcgcatta cgctgacggc aaacgaaccg atgagtgaaa aagacaagct
     5941 ctcttggctc atcctcaacc gcgccggcag cggcagcagc ggcgacaatg ccgccctgtc
     6001 tgcagccgca ggtgcgctgc ttgccgggca aatcaacgac cgcatcgggc tggtggatga
     6061 tttgggcttt accagcaagc gcagccgcaa cgcgcaaacc ggcgaactca accccgccga
     6121 acaggtgctg accgtcggca aacaactgac cggcaaactc tacatcggct acgaatacag
     6181 catctccagc gcggaacagt ccgtcaaact gatttaccgg ctgacccgcg ccatacaggc
     6241 ggttgcccgt atcggcagcc gttcgtcggg cggcgagctg acatacacca tacgtttcga
     6301 ccgcttctcc ggttcggaca aaaaagactc cgccggaaac ggcaaaggaa aataagcggt
     6361 tttcagacgg cgcgccgcca aaccggacat ttgaaaacct gcttttccac cgtccgccgc
     6421 cgccgtccgc ctgcaaggga acagaatcga tatagtg
//
LOCUS       DAWIVB010000121         6447 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWIVB010000121 DAWIVB010000000
VERSION     DAWIVB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.121"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            150..1691
                     /locus_tag="WIX60_001549"
     CDS             150..1691
                     /locus_tag="WIX60_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ5305799.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTAYRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     QTETL"
     gene            1720..2319
                     /locus_tag="WIX60_001550"
     CDS             1720..2319
                     /locus_tag="WIX60_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ5305800.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2316..2921
                     /gene="nadD"
                     /locus_tag="WIX60_001551"
     CDS             2316..2921
                     /gene="nadD"
                     /locus_tag="WIX60_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ5305801.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            2980..3366
                     /gene="rsfS"
                     /locus_tag="WIX60_001552"
     CDS             2980..3366
                     /gene="rsfS"
                     /locus_tag="WIX60_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ5305802.1"
                     /translation="MNEQELQDLQKMVGVAVNALGDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3420..3890
                     /gene="rlmH"
                     /locus_tag="WIX60_001553"
     CDS             3420..3890
                     /gene="rlmH"
                     /locus_tag="WIX60_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ5305803.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            3963..4229
                     /locus_tag="WIX60_001554"
     CDS             3963..4229
                     /locus_tag="WIX60_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ5305804.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4386..5069
                     /locus_tag="WIX60_001555"
     CDS             4386..5069
                     /locus_tag="WIX60_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ5305805.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPASAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5193..5705
                     /gene="coaD"
                     /locus_tag="WIX60_001556"
     CDS             5193..5705
                     /gene="coaD"
                     /locus_tag="WIX60_001556"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ5305806.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5737..6393
                     /locus_tag="WIX60_001557"
     CDS             5737..6393
                     /locus_tag="WIX60_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ5305807.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFDYQNRQIEIVAPLKNEWQTENIHRALEEFCMENKID
                     "
BASE COUNT         1400 a         1697 c         1860 g         1490 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttcagacggc gggaggaagg
       61 cggatacggt atgtagtcgg attgaaatat acggtatgtg ggggcttttg ggtacaatcg
      121 caacatcagt gtttcaagtg gggaaggcga tggatggacg gcgttgggtg gtatggggtg
      181 cttttgccct gctgccttcg gcttttttgg cggtaatggt cgttgcgcct ttgtgggcgg
      241 tggcggcgta tgacggtttg gcgtggcgcg cggtgctgtc ggatgcctat atgctcaaac
      301 gtttggcgtg gacggtattt caggcagcgg caacctgtgt gctggtgctg cctttgggcg
      361 tgcctgtcgc gtgggtgctg gcgcggctgg cgtttccggg gcgggctttg gtgctgcgcc
      421 tgctgatgct gccttttgtg atgcccacgt tggtggcggg cgtgggcgtg ctggccctgt
      481 tcggggcgga cgggctgttg tggcgcggca ggcaggatac gccgtatctg ttgttgtacg
      541 gcaatgtgtt tttcaacctt cctgtgttgg tcagggcggc gtatcagggg tttgtgcaag
      601 tgcctgcggc acggcttcag acggcacgga cgttgggcgc gggggcgtgg cggcggtttt
      661 gggacattga aatgcccgtt ttgcgcccgt ggcttgccgg cggcgtgtgc cttgtctttc
      721 tgtattgttt ttccgggttc gggctggcgc tgctgctggg cggcagccgt tatgccacgg
      781 tcgaagtgga aatttaccag ttggtcatgt tcgaactcga tatggcggtt gcttcggtgc
      841 tggtgtggct ggtgttgggg gtaacggcgg cggcagggtt gctgtatgcg tggttcggca
      901 ggcgcgcggt ttcggataag gcggtttccc ctgtgatgcc gtcgccgccg cagtcggtcg
      961 gggaatatgt gctgctggcg tttgcggcgg cggtgttgtc tgtgtgctgc ctgtttcctt
     1021 tgttggcaat tgttgtgaaa gcgtggtcgg ccggcgaatc gtggcgtgtg ttaatggaaa
     1081 gtgaaacgtg gcaggcggtg tggaatactt tgcgcttctc ggcggcggcg gtgtttgcgg
     1141 cggcggtttt gggtgtggtg tatgcggcgg cggcgcggcg gtcggcgtgg atgcgcgggc
     1201 tgatgttttt gccgtttatg gtgtcgccgg tttgtgtttc ggcgggcgtg ctgctgcttt
     1261 atccgcagtg gacggcttcg ttgccgttgc tgctggcgat gtatgcgctg ctggcgtatc
     1321 cgtttgtggc aaaagatgtt ttgtcggcct gggacgcact gccgccggat tacggcaggg
     1381 cggcggcggg tttgggcgca aacggctttc agacggcata ccgcatcacg ttcccccttt
     1441 tgaaaccggc gttgcggcgc ggtctgacct tggcagcggc aacctgcgtg ggcgagtttg
     1501 cggcgacatt gttcctgtcg cgtccggaat ggcagacgtt gacgactttg atttatgcct
     1561 atttggggcg tgcgggtgag gataattatg cgcgggcaat ggtgttgaca ttgctgttgt
     1621 cggcatttgc ggtgtgcatt ttcctgctgt tggacaacgg cgaaggcgga aaacagacgg
     1681 aaacgttata atgtgaaccc tttttcagag gacggcaaaa tgagcgagtt ggataacatc
     1741 cttgcccata accggcagtt tgtcgagtcg ggcgaatatg aaaaatactt taccgacaaa
     1801 taccccgaac gcgggctggc agttttgtcc tgtatggatg cgcggattat cgggctgctg
     1861 cccgacgcgt tgggtttgaa aaacggcgat gccaagctga ttaaaaatgc cggcgcgctg
     1921 gttacgcacc cgtggggttc ggtgatgcgg agccttttgg ttgccgtgtt tgaactgaag
     1981 gtcagagaga ttatggtcat cgcccatcac gattgcggta tgcaggggct gaatgccgaa
     2041 gaattcctcg ggcgcgtccg ggaaagccgg attcccgaag accgtatcga aaccctgcgt
     2101 tatgccggta tcgacctcga cggctggctg accggtttcg acaacgtcga agacagcgtg
     2161 cgccacacgg tggaccttat ccgtaaccat ccgctgatgc cgcgccatat cgccgttcac
     2221 ggactggtca tccatcccgt taccggcaaa ctgacgctgg ttgtggacgg cagtgtttca
     2281 gacggcatgg acttatcgga aggaatggaa acatcatgaa gaaaatcgga ttgttcggcg
     2341 gtactttcga cccgatacac aacggacatc ttcatatcgc ccgtgccttt gccgacgaaa
     2401 tcggcttgga cgcggttgtt ttcctgccga caggcggccc gtatcacaaa gacgcagcct
     2461 ccgcttccgc cgccgaccgc cttgccatgg tcgaattggc gacggcagaa gacgcgcgtt
     2521 ttgccgtcag cgattgcgac atcgtccgag aaggtgcaac ctatactttt gataccgtcc
     2581 aaatcttccg ccagcagttc ccatccgcgc aactctggtg gctgatgggc agcgacagcc
     2641 tgatgaagct gcacacatgg aaaaaatggc agatgctcgt gcgcgaaacc aatatcgccg
     2701 tcgccatgag gcagggcgac agcctgcacc aaaccccgcg cgaactgcac gcgtggctgg
     2761 gcaagtccct tcaggacggc agcgtccgca tcttgtccgc cccgatgcat aatgtgtcgt
     2821 caacggaaat ccgccgcaac cttgccggcc aaggcgtttc agacggcatc ccgcctgccg
     2881 ccgcacgcta catccgcgaa cacggtttgt atgaaaaata aagtcaaatc agtaagaaac
     2941 ggctataatg ccgtctgaaa acatcccgtt aggaaaataa tgaacgaaca agaactgcaa
     3001 gacctgcaaa aaatggtcgg ggtcgccgtc aacgccctcg gagacatcaa agccaaagac
     3061 atttccgttc tcgaaaccca agacaaaact tcgctgtttg ccagaatgat tatcgccagc
     3121 ggcgacagta cgcgccaagt caaagcactg gccaacaacg ttgccgtcga tttgaaagaa
     3181 gccggttttg aaatcctcag taccgaaggc gacagcggcg aatggacgct ggttgatgca
     3241 ggagacctcg tcgtccacgt catgctccct gccgtgcgcg acttctacga catcgacacc
     3301 ctctggggcg gcgagaaacc gagtttccac gccggaatgc agaagccgtg gcacgcggca
     3361 gactgattcc cgatgccgtc tgaaccttca gacggcattt tcctgtaagg gagaaagcat
     3421 tgaacatcac cgttttggca gtcggcacca aaatgccgcg ctgggttgat gaggccgtcg
     3481 ccgaatacgc caaacgcttc ggacgcgacg tcgcctacgc actcaaagaa atcaaacccg
     3541 aaaaacgcgg cgcgggcgtg aatgccgccc aaggtatggc ggcggaagaa aaacgcatcc
     3601 ttgaagccat tccgcaaggc gcgttcctcg tcgttcttga cgaacgcggc aaagcaccga
     3661 cctccgtcga gctggcggaa cacctcaaaa gctggcggca aaacggcgaa cacgtctgct
     3721 tcgtcatcgg cggcgcggac ggcatgaccg accgcctcaa acagcaggca cgcatgatga
     3781 tgcgcctgtc cagcctgacc ctgccgcacg gcatggtgcg cgtctttctg accgagcagc
     3841 tctaccgcgc cgtttccatc ctgcacaacc atccctatca tcgggaataa gaaggctttt
     3901 gtccgtatcc ctaatcggtt aaaatcaccc cgttatttct gaacacagca aaggaatccg
     3961 ctatggcacg catggtattc tgcgtcaagc tcaacaaaga agccgaaggc atgaaatttc
     4021 cgccgcttcc caacgaattg ggcaagcgca tttttgaaaa cgtatcgcaa gaagcgtggg
     4081 cggcgtggac gcgccaccaa acgatgctga ttaacgaaaa ccgtttaagc ctcgccgatc
     4141 cgcgcgcgcg cgaatacctg gctcagcaga tggagcagta tttcttcggc gacggcgcgg
     4201 atgccgttca gggatacgtt ccgcaataac ggttttccgt tttgaacaca ggctgtccga
     4261 aactgcttca gacggccttt aaaatacgcc ggacaacttt atcttcacgc aaccggacaa
     4321 attttgacat tgggcaatgt taaaatcccc cattattccc aaccgcgctt tcaggagcag
     4381 atgatatgca acacgacgtt tacgactaca ccgcgcatac ggtttctaaa aacaccgtcc
     4441 tgcagaaaac ctaccgcctg ctcggatttt catttattcc ggcttccgca ggcgcggcac
     4501 ttgccgccaa tgccggtttc aatttttacg ccgccttcgg ttcgcgctgg atagggtttg
     4561 ccgtcgtgtt ggcgtttttc tacggtatga tccacttcat cgagaaaaac cgttacagca
     4621 ataccggcgt taccctgctg atggtattca cattcggtat gggcgtattg atcggccccg
     4681 tgctgcaata cgcgctccat attgccgacg gtgcgaaaat cgtcggcatt gccgccgcga
     4741 tgaccgccgc cgtcttttta acgatgtccg ccttggcgcg ccgaacccgg ctcgatatga
     4801 acgcgctcgg acgcttcctg accgtaggtg cggtcattct gatggtcgcc gtggttgcca
     4861 atctgttttt gggtattccc gcactcgccc tgaccatttc cgccggtttt gtcttgttca
     4921 gttccttaat gattatgtgg caggtacgca ccgtcatcga cggcggcgaa gacagccaca
     4981 tcagcgcggc actgacactg tttatctcgc tttacaacat cttcagcagc ctgctgaaca
     5041 tcctgttgtc tttaaacggc gaggattgat gccggtaaaa cgccgtccga ctatgccgtc
     5101 tgaaaatgct tcagacggca ttttactttg ggcatacaat acggatgcac acgcaccaac
     5161 acacagccga ctatgaagcc gggaggaatc aaatgttacc gaatacaccg cgccgcgccg
     5221 tttatgccgg cagtttcgat ccgcccacat tggggcatct gtggatgata cggcaggcgc
     5281 aatctatgtt tgacgaactc atcgtcgcca tcggcattaa ccccgacaaa cgcagcacct
     5341 ataccgtcgc tgaaaggcag gatatgttgt gcgatattac taaaatgttt cccaacgtca
     5401 gaaccgatgt atttgaaaac cgatttctgg tgcattacgc ccgtgaggta gatgcaggat
     5461 tcatcgtgcg cggcatccgt tctgcttcgg attacgaata cgaacgttcc atgcgccata
     5521 tcaacagcga cctcgccccc gaaatatcca ccgtattcct catgccgccg cgcgaaatcg
     5581 ccgaagtgtc gtccactatg gtcaaaggac tggtcgggcc ggaaggctgg acggaaacga
     5641 tccaccgcta cgtgccgcaa gctgtgtacg aaaaaatcct tgccgaacat caacacgaaa
     5701 attgaaccct tctatttttg caaggcatat atggatatgt tggaaatttt gttccgccat
     5761 caagacttcg tcgccatcaa caaaccgggc ggcatatccg tccaccaaga cagcggcgag
     5821 accggactgg cccgaacact cgccatacag ttgggcgtag agcgtgtgtg gctgctgcac
     5881 cgattggaca aacagaccag cggcatttta ctgtttgccc tcaacaaaga aagcgcgtcc
     5941 gcccttagcg gacagtttgc cggcaaaagc atcaaaaaaa cctatttggc actgtccgac
     6001 cgcaagccgt ccaaaaagca gggttggatt aaaggcggaa tggaaaaatc cagatgtgga
     6061 atgtggaagt tgacgcgcaa tacagaaaat atcgccgtta cccgattcca cagcatcagt
     6121 atcgccgaaa aactgcggct gttcatccta gaaccgcata cgggcaaaac gcaccaattg
     6181 agagtggcga tgaaaagttt gggcagtccg attctgggcg acagcttata cggcggaaca
     6241 gaatccgaaa ccatgtttct gtatgcatgg aaaatacaat ttgactacca aaaccgacaa
     6301 atcgaaattg ttgcaccctt aaaaaacgaa tggcagactg aaaatatcca tcgggcactg
     6361 gaagaattct gtatggaaaa taaaattgac tgatataaat taaatttata aacaaataga
     6421 taaatatttt taaaaaaatt tcttcag
//
LOCUS       DAWIVB010000122         6266 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWIVB010000122 DAWIVB010000000
VERSION     DAWIVB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.122"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..1184)
                     /locus_tag="WIX60_001558"
     CDS             complement(<1..1184)
                     /locus_tag="WIX60_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ5305808.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WR"
     gene            complement(1189..1842)
                     /locus_tag="WIX60_001559"
     CDS             complement(1189..1842)
                     /locus_tag="WIX60_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ5305809.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            1993..2283
                     /gene="gatC"
                     /locus_tag="WIX60_001560"
     CDS             1993..2283
                     /gene="gatC"
                     /locus_tag="WIX60_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ5305810.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            2346..3791
                     /gene="gatA"
                     /locus_tag="WIX60_001561"
     CDS             2346..3791
                     /gene="gatA"
                     /locus_tag="WIX60_001561"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ5305811.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            3788..4720
                     /locus_tag="WIX60_001562"
     CDS             3788..4720
                     /locus_tag="WIX60_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ5305812.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDSLKTEARGISDGIQTEKPPRRTA"
     gene            4763..6193
                     /gene="gatB"
                     /locus_tag="WIX60_001563"
     CDS             4763..6193
                     /gene="gatB"
                     /locus_tag="WIX60_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ5305813.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKLTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGEIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
BASE COUNT         1606 a         1899 c         1576 g         1185 t
ORIGIN      
        1 aaccgccaag cgcgttcggt atcggcggta taaatgcagg caccgagccc gaacggggaa
       61 tcattggcaa gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc
      121 ccgaatactt cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg
      181 ggataaaacc agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt
      241 gaaacggcat cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt
      301 gcaagcgtag tatcgggatg tttggggtcg cccattttca atttagcgca ttcggcaaga
      361 aacagcgtga taaaacgatc ggctgcggct tcggttacga tgatgcgctt ggcggcgtta
      421 cacgattgcc ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc acgctccaaa
      481 tcagcatcgg gcatcacgat aaaggcgttg ctaccgccga gctccaacac ggttttctta
      541 aggtttgcgc ccgcgtgtgc cgcaaggatg cgccccgtat gcgttgaacc ggtaaacgcc
      601 attgcatcgg tatcttcaac cgccttgagc gtgcccgcct catccagcca cacgcctgcc
      661 agaggaatgc cgtctgaagc caaatcgaac agtgcctgac tgacgcgtgc cacgctgggc
      721 gcgggtttga cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc
      781 tgccagacgg gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcgaagcgc
      841 acctgactca aactcgcctg cgtcgcgatg gttttgtggg caagcagttc gggggcaagg
      901 cgggcgtaat agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcaag
      961 cagcgtccga cttcctcaca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg
     1021 tcggcaaatt tttgcaggcg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg
     1081 ccgcgcattt tcaaatccgc cagccgccgt tcaaactccg cataatcttg agcggggcgg
     1141 cggtaaagcg tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag
     1201 ttgcgggaat atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat
     1261 catacccata atgcggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt
     1321 cccggcaacg attaaaatgg cataacaaat cgcactgacc accaaaccgg ccgcgataat
     1381 caacgcgatg tcgccgtatg ttttagccgc cgaagcgtaa ataatcacgg tcgaaatacc
     1441 gcccgggccg atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg
     1501 ggcggggcgc gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc
     1561 gttgccgttc atcatcgaaa tggcgatcag cagcaccaaa atcccgccgc cgacctgaaa
     1621 cgaaccgacg ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc
     1681 aatcacggca aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct
     1741 gtgcccgttg gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa
     1801 aaaagccaca atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa
     1861 acccgtgcgg caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa
     1921 ttaaaaattc aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca
     1981 aggatgcttt tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga
     2041 ctgcacctga ctgcggaaga aaaagaaaaa tcgcttcaag aattaaacga cattttcact
     2101 atggtcgaac agatgcaaac cattaacaca gacggcatcg aaccgatggc gcacccgcac
     2161 gaggccgccc tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat
     2221 caggcgggtg ctccggaagt acgcaaccgt ctgtacatcg taccgcaagt tatcgaagaa
     2281 taatccgaat atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa
     2341 gatttatgac ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt
     2401 ccgccgtcga actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca
     2461 acggctatat caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac
     2521 gtatcgcgca gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt
     2581 tctgccaaac cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc
     2641 cctacaccgc caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca
     2701 ccaatatgga tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggtgcagcca
     2761 aaaacccatg gaatcttgag cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg
     2821 ttgccgcgcg cctcgcccct gccgcgctcg gttcggacac cggcggctct atccgccaac
     2881 ccgcatcgca ctgcggcatt accggcatca aacccacata cggcacggtt tcccgcttcg
     2941 gtatggtcgc ctacgcctcc agcttcgatc aaaccggccc gatggcgcaa actgccgaag
     3001 actgcgcgat tctgttaaac gcgatggcag gtttcgaccc caaagactcc accagcctcg
     3061 agcgcgaaaa agaagactac acccgcgatt tgaaccaacc gctcaaaggt ttgaaaatcg
     3121 gcctgcccaa agaatatttc ggcgaaggca acagcgccga tgttctgacg gcattgcaaa
     3181 acaccattga tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa
     3241 ccaagctgtc catccccgcc tactacgtcc tcgcctccgc agaagccagc accaaccttt
     3301 cacgttacga cggcgtacgt tacggacacc gtgccgccca attcgccgat ttggaagaaa
     3361 tgtacggcaa aacccgcgcc gaaggtttcg gcagcgaagt caaacgccgc atcatgatcg
     3421 gcacttatgt actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc
     3481 gccgcctcgt tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc
     3541 cgaccgcacc cactgcagcc ccaaaaatcg gagcggatgc ttcgccggtt gaaacctact
     3601 tgagcgatat ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg
     3661 caggcttcag cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg
     3721 aagccaaaat cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac
     3781 gacccgaatg aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta
     3841 tgcgccttta tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa
     3901 agaaatttac gccttcgagc agtttgtcaa cgcatcccct atccgtcagg ggctgttcct
     3961 ccactgcccg caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg
     4021 caaacgccgt ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac
     4081 agacatcctg caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga
     4141 cggcatcagc ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt
     4201 atctttgcgc gacgaagcag gcaaccggct gtatatggcg actttcgcct ttgtcggcac
     4261 acacctgctg acagcctccg tacaagggcc ggcgggtgaa gaagccaaag acaccgtccg
     4321 ccgcataacc aaacaactcc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca
     4381 atatttcgcc gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt
     4441 caaactgcgc tggaaactta aaaagcgcgt caaaatgaat tacgacgcat tctggcagga
     4501 atacggcgca agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa
     4561 agaccttgcc gacatcgaaa gcaaaaagcg ttcgatgtac cgcaagcgtt atgaaatgct
     4621 ggacaatatg gttgcagaga tgaaagacag tctgaaaaca gaagcacgcg gcatttcaga
     4681 cggcatccaa acggaaaaac cgccccgccg gacagcctga cgcgaagact atcgaattga
     4741 tattttagag aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt
     4801 ccaattgaac accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc
     4861 caacgcgcac gccagcgtag tggaatgcgc gctgccgggc gttttgcctg tgatgaaccg
     4921 tgaagtcgtt gaaaaagcca tcaaattggg tttggcttta gatgcgaaaa tcaatcagaa
     4981 aaacgtgttc gaccgcaaaa actacttcta tcccgactta ccaaaaggtt atcaaatcag
     5041 ccagttggac ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt
     5101 gaaaaccatc aacgtaaccc gtgcgcacat ggaagaagac gcaggcaagt ccgtgcatga
     5161 aggcttgaac ggcgcaaccg gtatcgacct gaaccgcgcc ggcacgccgc tgttggaagt
     5221 ggtatccgaa cctgaaatgc gttccgccgc cgaagccgtt gcctacgcca aggccttgca
     5281 cagcttggta acctggctgg acatttgcga cggcaatatg gcggaaggct cgttccgcgt
     5341 cgatgccaac gtatccgtgc gcccgaaagg tcaagaagag ttcggcacgc gccgcgagat
     5401 taaaaacctc aattccttcc gtttcttgga gcaggcgatt aattacgaag cggaagcgca
     5461 aatcgagatt ttggaagacg gcggcaaagt acagcaggca accatgctgt ttgatcccga
     5521 aaaaggcgaa acccgcgtaa tgcgcctgaa agaagatgcg cacgactacc gctacttccc
     5581 cgaccctgat ttgctgcccg ttatcatttc agacgcccaa atgcaaaaag ccaaagcaga
     5641 aatgcccgag ctgccgaaag aaatggcagc gcgtttcgtg gcggattacg gcgtgtccga
     5701 atacgacgcg cgcctgctga ccgcaagccg tgcgcaggct gcctattttg aagaagccgc
     5761 caaagaaagc ggacaaggca agctgactgc caactggatg aacggcgaac ttgccgccgc
     5821 gctgaacaaa gaaggcatgg aacttgccga cagcccgatt accgccccgc gcctcgccgc
     5881 gctggttggc aaaatcgccg acggcacatt aagcagcaag ttagcgaaaa aagcctttga
     5941 agccatgtgg gcagaacccg aagccaccat tgccgaaatc attgaaaaac acggtttgca
     6001 acagatgacc gacaccggcg agattgaagc catggtggac gaagtgctgg caaacaacgc
     6061 caaagccgtg gaacagttta aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca
     6121 agtgatgaag gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc
     6181 caaactggct taatccgtta tcacacaggt cgtctgaaag caaagttcca acgaaggtaa
     6241 aacaggaaat aagctttcag acggcc
//
LOCUS       DAWIVB010000123         6258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWIVB010000123 DAWIVB010000000
VERSION     DAWIVB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.123"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            138..1973
                     /gene="edd"
                     /locus_tag="WIX60_001564"
     CDS             138..1973
                     /gene="edd"
                     /locus_tag="WIX60_001564"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ5305814.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGRGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLSAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLIDEAE
                     WNAREVEHIDLGANQQGCGRELFAGFRSLTGGAETGAMSFGGEFA"
     gene            2081..2719
                     /locus_tag="WIX60_001565"
     CDS             2081..2719
                     /locus_tag="WIX60_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ5305815.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(2975..4016)
                     /locus_tag="WIX60_001566"
                     /pseudo
     CDS             complement(2975..4016)
                     /locus_tag="WIX60_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            4179..5219
                     /gene="mutY"
                     /locus_tag="WIX60_001567"
     CDS             4179..5219
                     /gene="mutY"
                     /locus_tag="WIX60_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ5305816.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            5636..6196
                     /locus_tag="WIX60_001568"
     CDS             5636..6196
                     /locus_tag="WIX60_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ5305817.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         1604 a         1768 c         1515 g         1371 t
ORIGIN      
        1 ctatagtgag gttttcagac gaccccttgt aaggtttgaa aggtcgtctg aaacgccaaa
       61 tgccccacac ccaatacaaa aaatcagaaa cgcgcaaaca aaatccccat accccccgtt
      121 ccgacaggag accgaccgtg aacactactc ctatccactc caaactcgcc gaaatcaccg
      181 ggcgcattat cgaacgcagc cgtccgacgc gtgaaaaata tctggcgaaa atccgcagcg
      241 ccaaacaaat gggacgcttg gagcgcaacc aactcggctg cagcaatttg gcgcacggct
      301 atgccgccat gcctaaaagc atcaaaatcg aaatgcttca ggaaactgtc cccaacttag
      361 gcatcatcac cgcctacaac gacatggttt ccgcacacca gccgtttaaa gacttccctg
      421 accaaatcaa agacgaagcg cagaaaaacg gcgcgaccgc ccaagtcgcc ggcggcacac
      481 ccgccatgtg cgacggcatc actcaaggct acgccggtat ggaactgtcg ctgttctccc
      541 gcgacgtgat tgccatgagt accgccgtcg ggctgtcgca tcaaatgttt gacggcagcc
      601 tgtttatggg cgtatgcgac aaaatcgttc caggtttgat gataggcgcg ctttcgttcg
      661 gtcatattcc gggtatcttc gtccccgcag gcccgatgtc cagcggtatc ggcaacaaag
      721 aaaaagcccg cacccgccag cttttcgccg aaggcaaggt cggacgcaac gaacttttga
      781 aaagcgaaat gggttcttac cacagcccgg gcacctgcac tttctacggc acggcaaact
      841 ccaaccaaat gatgatggaa atgatgggcg tgcacctgcc tgccgccgcc ttcgtccacc
      901 cttacaccga cctgcgcgaa gcgctgaccc gctacgccgc cggacacctc gcgcgcggca
      961 tcaaaaacgg cacgattaaa cctttgggcg aaatgttgac cgaaaaatcc tttatcaacg
     1021 ccttgattgg cctgatggca accggcggtt cgaccaacca caccatgcac ctcgtcgcca
     1081 tggcacgcgc cgccggcgtg attttgaact gggacgactt cgacgaaatt tcctccatca
     1141 tcccgctgct cattcgcgtt tatccgaacg gcaaggccga cgtgaaccac tttaccgcag
     1201 cgggcggact gcctttcgtt atccgcgaat tgctgaatgc aggcctgttg cacgacgatg
     1261 tcgataccgt cgtcggacgc ggtatgcgcc actacaccaa agagcctttc ctcatcgacg
     1321 gcaaactcga atggcgcgaa gcccccgaaa ccagcggcaa cgacgacatc ctgcgcaaag
     1381 ccgataaccc gttctcccca gacggcggtc tgcgcctgat gaaaggcaac atcggtcgcg
     1441 gcgtgattaa agtgtccgcc gtgcgcgaag gctgccgcat catcgaagca cctgccatcg
     1501 tgttcaacga ccaacgcgaa gtattgtccg catttgaacg cggcgagttg gaacgcgatt
     1561 tcgtctgcgt cgtccgctac caaggcccgc gcgccaacgg tatgcctgaa ttgcacaaac
     1621 tgaccccgcc tctgggcatc ctgcaagacc gcggcttcaa agtggcgttg ctgaccgacg
     1681 gccgtatgtc cggcgcgtcc ggcaaagtgc ccgcatccat ccatatgacc cccgaagccc
     1741 tgatgggcgg caacatcgcc aaaatccgta ccggcgacct gatccgcttc gattccgtga
     1801 ccggcgaact caacgtcctg attgacgaag ccgaatggaa tgcccgcgaa gtcgaacaca
     1861 tcgacttggg cgcgaaccaa caaggctgcg gccgcgaact cttcgccggc ttccgcagtt
     1921 tgaccggcgg cgcggaaacc ggcgcgatga gcttcggcgg cgaatttgcc tgatgcgcgt
     1981 ttcagacggc cttcgtgaat agaaggtcgt ctgaaaaatt aaatccccat cccgtttgac
     2041 cgcagcaaaa acaacacccc attccacccg gagaaccgaa atgtccaaac tgaccccccg
     2101 cgaaattttg accgccggcg cagttgtgcc ggtaatggcg attgacgact taagcaccgc
     2161 cgtcgatttg tcccacgccc ttgtcgaagg cggcatcccc accctcgaaa tcaccctgcg
     2221 cacccctgtc ggcctcgatg ccatccgcct gattgccaaa gaagtgccca acgccatcgt
     2281 cggcgctggt acggttacca accctgaaca gctcaaagcc gtcgaagacg caggcgcggt
     2341 tttcgccatc agcccgggtt tgcacgaatc cctcgccaaa gccggccaca acagcggcat
     2401 ccccctgatt cccggtgttg ccaccccggg cgaaatccaa ctggctttgg aacacggcat
     2461 cgacaccctc aaactcttcc ccgccgaagt cgtcggcggc aaagccatgc tcaaagccct
     2521 ttacggccct tacgccgatg ttcgcttctg cccaaccggc ggcatcagcc tggcaactgc
     2581 ccccgaatac ctggcactgc ccaacgtcct gtgcgtcggc ggctcttggc tgacaccgaa
     2641 agaagccgtg aaaaacaaag actgggacac catcacccgc ctcgccaaag aagcggcggc
     2701 attgaaaccc aaagcctgaa ccgcatcgta aaaatgccgt ctgaaaaacc tttcccgttt
     2761 cagacggcat tttgtcgatt gagggcacag tcggcataca cggcagcact gatcagacat
     2821 accgccccta aaatgcccat ccgccttccg cataataaaa ataacggtca gttcattcga
     2881 cagcagccgg acagcccact acacggctga aaaaatgccg tctgaaacgc attcagacgg
     2941 catccgctta aaaaaacaac tgattcaacg ccgattaatc cgcttccaaa accactttca
     3001 tcgcttggtt ttcggcggcg tgtttgaaca cgtcgtaggc tttttccaat tcgctgaatt
     3061 tgaaacggtg ggtcaacatt ttggtgtaat cgacggagct gctggaaatt gccttcatca
     3121 gcatttcggt ggtattggcg tttaccaggc cggtagtgat ggcaagcttt ttaatccaca
     3181 gtttttccag tttgaaatca acggattgac cgtgtacgcc gacaacggcg atatggccgc
     3241 cgggtttcac gatgtcttgg cacatattcc aagttgcagg gataccgacc gcttcgatgg
     3301 cacaatctac gccgtcttca ccgacgatgg cgaaaacttg tttggagacg tcgccggaag
     3361 cggggctgat ggtatgggtc gcgcccaact ctttcgccag tttcaaacgg ttttcgtcca
     3421 tatcgcacac gataatggcg gcggggctat acagttgggc ggtcaacaag gcggacatac
     3481 cgacaggagc ggcacctgcg atgaatacgg tgtcgccggg tttgacatcg ccgtattgca
     3541 cgccgatttc gtgtgcggtc ggcaaagcgt cgctcaacaa cagggcgact tcttcgttga
     3601 cgttgtcggg cagcggcacg aggctgttgt cggcataagg cgtgcggacg tattctgcct
     3661 gcgtgccgtc aatcatgtag cccaaaatcc agccgccgtt gcggcaatgc gaataaagtt
     3721 ggattttgca gttgtcgcaa gtacagcatt tgctgacgca tgaaataatg actttatcgc
     3781 cgactttgat gttttttaca gcctcgccga cttcttctac aataccgatg ccttcatgac
     3841 cgagaatacg accgtcggcg acttcgggtt tttttgcctt tccaaatacc caaatcggta
     3901 ccgcaaatcg tggttttgac gattttcacc accgcatcgg tcggatcgat aatctgcgga
     3961 cggggttttt cttcaaaacg gatgtcgttt gcgccgtgat aaaccattgc tttcatgctg
     4021 atactccttg cttgttgata aataatttca ataccgcaat aaagtttctt tatatgagtt
     4081 atatgcccct acaaaaaata agtcaataag aattattttc acaatgttat acaataacat
     4141 actgttttaa atataaataa aaccaccgat tgatattaat gaacacaccc atccccttct
     4201 ccgaacggct catccgctgg caaaaacaac acggtcgcca ccacctccct tggcaggtca
     4261 aaaaccctta ttgcgtctgg ctttccgaaa tcatgctcca gcaaacgcag gtcgccaccg
     4321 tgttggacta ctatccgcgt ttcttagaaa aattcccgac cgttcagacg cttgccgccg
     4381 cgccgcaaga cgaagtgttg tcgttgtggg cgggcttggg ctattacagc cgcgcgcgca
     4441 acctgcacaa agccgcgcaa caagtcgtca ggcaattcgg cggcacgttt ccgtcggagc
     4501 gcaaagactt ggaaaccctc tgcggcgtag gcagaagcac cgccgccgcc atttctgcct
     4561 tctccttcaa ccgccgcgaa accattttgg acggcaacgt caaacgcgta ctctgccgcg
     4621 tgttcgcccg cgacggcaat ccgcaggaca aaaaatttga aaactcgctc tggacacttg
     4681 ccgaaagcct gctgccgtct gaaaacgccg atatgcctgc ctatacacaa ggtttgatgg
     4741 atttgggcgc gaccgtgtgc aaacggacga aacccttgtg ccaccaatgc ccgatggcgg
     4801 acatctgcga agcaaaaaag caaaaccgca ccgccgagct gccgcgcaaa aaaaccgccg
     4861 ccgaagtacc gaccctgccg ctttactggc tgattgtccg caaccgggac ggcgcgattt
     4921 tgctggaaaa acgccccgcc aaaggcattt ggggcgggct gtattgcgtg ccgtgttttg
     4981 aaagtttgaa cgggctttcc gactttgccg ccaaattctc cctgaccatg gcagatatgg
     5041 acgaacaaac cgccctgacc caccgtctga cgcaccggct gctgatgatt acgccgtttg
     5101 aaggacaaat gccgtctgaa agcccttcag acggcatttg gataaagccg gcgcatttga
     5161 aagattacgg cctgcccaag cctttggaaa tttatttaaa cggtaatagg ttagaataaa
     5221 caaaataaac ccattgaact gttgtttgca ggtatcgcag caagaacaac cgatgaattt
     5281 gggtcgtatt ttaggcggcg ggataatgtt caaatgggac atttggaacg gaagaagtcg
     5341 gcaatttaaa aaggatttaa aaagcaaaga aggtcaaaaa catgaacaca aacttaaatg
     5401 acaaagacaa agccatggat accgcaatca ggtttcagaa aaggatgagg attccgaaat
     5461 ttttcttttt aattctctga atcacaatgg ttttggcatt tatccaagac gtgataacgg
     5521 gttctaattt tctgcaaata acaattaatg taaaattttc gtaaaaattt atcggctttt
     5581 aaaacaaaat tgactaaaat agtcgcgagt ttttactgca ataaaggaga ttgcaatgaa
     5641 tatgaaaacc ttattagcac tagcggttag tgcagtatgt tcagttggtg ttgcgcaagc
     5701 acacgagcat aatacgatac ctaaaggtgc ttctattgaa gtgaaagtgc aacaacttga
     5761 tccagtaaac ggtaacaaag atgtgggtac agtgactatt actgaatcta actatggtct
     5821 tgtgtttacc cctgatttac aaggattaag cgaaggctta catggtttcc acatccatga
     5881 aaacccaagc tgtgagccaa aagaaaaaga aggtaaattg acagctggtt taggcgcagg
     5941 cggtcactgg gatcctaaag gtgcaaaaca acatggttac ccatggcaag atgatgcaca
     6001 cttaggtgat ttacctgcat taactgtatt gcatgatggc acagcaacaa atcctgtttt
     6061 agcaccacgt cttaaacatt tagatgatgt tcgcggtcac tctattatga tccacacggg
     6121 tggtgataat cactccgatc atccagctcc acttggcggt ggcggcccac gtatggcatg
     6181 tggcgtgatt aaataattcg attgttcgaa acgaaaagtg cggtgaattt tgaccgcact
     6241 tttttgctag atatttag
//
LOCUS       DAWIVB010000124         6237 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWIVB010000124 DAWIVB010000000
VERSION     DAWIVB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.124"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WIX60_001569"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WIX60_001569"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ5305818.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKTVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGGDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1297..2652)
                     /locus_tag="WIX60_001570"
     CDS             complement(1297..2652)
                     /locus_tag="WIX60_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ5305819.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(2667..2939)
                     /locus_tag="WIX60_001571"
     CDS             complement(2667..2939)
                     /locus_tag="WIX60_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ5305820.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3065..3802)
                     /locus_tag="WIX60_001572"
     CDS             complement(3065..3802)
                     /locus_tag="WIX60_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ5305821.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVVAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WIX60_001573"
     CDS             complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WIX60_001573"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ5305822.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5127..5720
                     /locus_tag="WIX60_001574"
     CDS             5127..5720
                     /locus_tag="WIX60_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305823.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5832..>6237
                     /locus_tag="WIX60_001575"
     CDS             5832..>6237
                     /locus_tag="WIX60_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Caudovirus prohead protease"
                     /protein_id="HEZ5305824.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGRTFFVAHIVDPVAVTKVKTGVYKGFS
                     IGGSVTAHDELNKSQITGLKLTEISLVDRPANP"
BASE COUNT         1685 a         1573 c         1459 g         1520 t
ORIGIN      
        1 tttgagaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaaggtt ctttaatatt ttaaaagcct tgccgttgga ttataatccc
      121 ccaaccgatt tcttaatttt gctaataaac acttgcttgg taaggaatga atttatgcgc
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcggat tttgaaggaa
      241 atgcacggag gcaaactgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcgg aatacgaagc ggtcgaacaa tactcgcttt ggccggcagt cggaaaccaa
      481 tggcagcttg aggctttgct gatccgccat tggaaaaaaa ccgtcaaagt ctggttgaaa
      541 atggattcgg ggatgcaccg taccggtttt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaagc agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat gacggaaata cagatggaag cattcgattt gggtacggaa
      721 gggctggaag gcgaagaaag ccttgccaac tccgccgcta ttttgaatgt tcccgaagca
      781 cgcagggact gggggcgcgc cggtctggcg ttatacggca tttccccgtt cggaggaggc
      841 gatgacaggc tgaagcccgt gatgaggctt tcaacccgta ttttcggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgcgtc
      961 ggcctgattg cctgcggtta tgcggacggt tatccgcgcc gcgccccaag caattccccc
     1021 gtcgctgtcg acggcaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg atgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca ataccgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taatcaagtc caaacaaaaa tgccgtctga
     1261 agcctttcag acggcatttc cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg
     1321 tgcgggaatc ctgccgcccc ggttgacgaa cacttcgcat gagccttctt tgaccggcat
     1381 tacaggcgcg tagcccaaca ggccgccgaa ctcgacgctg tcgccgacgg ttttaccggt
     1441 taccggaata atgcgcacgg cagtggtttt gctgttgatc atgccgatgg cggcttcgtc
     1501 ggcaatgatg ccggaaatgg tgtgcgcggg tgtgtcgccg ggaacggcaa tcatatccaa
     1561 gccgaccgaa caaacggcgg tcatcgcttc gagtttatcc aacgtcagca cgcctgcttc
     1621 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccac tcaaaccgcc
     1681 aaccgcgctc gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     1741 tgctgttgtg ccgtgcgtac cgcagacgct caaacccatt tcttcaagaa tgcgcgccac
     1801 cgagtcgccg acggcagggg tcggtgccag cgacaagtcg agaataccaa acgggatatt
     1861 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtaattt tgaaggcggt
     1921 tttcttcaca acttcggcaa cttcggtcaa tgtcgttgca tccgaatttt ccaacgcggc
     1981 ttttacgaca cctgggccgg atacgccgac attaatcaca gcatccgctt cgcccgatcc
     2041 gtgaaacgca cccgccataa acggattgtc ttccaccgca ttgcagaaca cgacgatttt
     2101 cgcgcagccg aaaccttcgg gcgtaatttc agccgtgcgt ttgatggttt cgcctgccag
     2161 cttgaccgca tccatattga taccggcgcg cgtactgccg acattgatgg agctgcacac
     2221 gatgtcggta gtcttcatcg cttcgggaat ggaacggatt aacacctcgt ctgaaggcga
     2281 catgcctttt tgcaccaacg cggaaaagcc gccgataaag gacacgccga tggctttggc
     2341 agccttatcc aaagtttgcg ccacgctgac ataggaatcg gcatgggtag ccgccgcgat
     2401 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcaga
     2461 cagatatttt gccgtcgcca ccaagtcttt gccgaccgtg gtaattttgt tgtaaatgtt
     2521 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     2581 ggtgcggaca tcgaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     2641 ttggatactc atcacattcc tccgactcaa atgcggtgca tcgcttggaa gatttcttca
     2701 ttttgcatac ggatatcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaacc
     2761 tcttgacgcg atttgctgca ttttgaagtg tccaccaaga taatcatagt aaaaaaatcg
     2821 tccatcagct gttggctgat gttgagaata ttgatttggt tttccgccaa aattttggaa
     2881 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     2941 ggcttactcc ttgcagatat ccgttaaagt ccgaaattat accaccgttg gattttgaag
     3001 aaatattgtc aacaatatat acatacaaaa tgccgtctga aactatttca gacagcatca
     3061 agattcaggg ttcgattaaa taaccatcct tatcccactg ggttttcctg accaacttgt
     3121 catcctgata aacagcttcg ctctttttag aaccatcttc ataccactcc aaaaccaccc
     3181 cgttgcgttg atggtggcgg atagacagtt ccgagagtaa tcggccgctt tcatcccaag
     3241 tcagaatttt ggcaggctca tcgttgacca taaccatttc cgtcttgata ctgccgtcgg
     3301 cataccattg cttccatacg ccgtttgcct tattttgctt aaactggatt tcgctttcct
     3361 tgccgccgtt acggtaatag cggtatcccg taccctcact caagccattt ttataaggca
     3421 taacggcaga ttttttaccg ttcggatacc agttgaccca ctccccgtcc ggcttaccct
     3481 tgctgaagcc ccccgccatt tttttctgac cattaaaatg ccacaaaatc aacataccgt
     3541 tttgcagggt aggcacaaaa gatttgattt gcgttgaagc aacgatataa ggttcagaat
     3601 atttcttcat cgacggataa taaaaatcct gcgcgtgcgc aatacccgcc accacactat
     3661 attgcctgat ataagcggca gaagacatcg tcgccgtcag ctttccgttc tgattaaaat
     3721 aaacagaata ggtctgcgcc ggcaaagcgg ccgaaaaacc caacaggaca gttgaaaata
     3781 caatccgaga taattttttc attgcaatag cgatataaaa acaaggctgt gttttagtaa
     3841 tctgttgatt tcaattattt gcaagggaaa agacaattat tttccggtta ggaataaacc
     3901 tattctattg aatatattga agccaagtac gcctatcaac actatattaa aacactgcca
     3961 aaaacaatta acttataaac aatatggtaa ggatttctct gccaagcatc aaacccgaga
     4021 caacgtatcg taaaaatgcc gtctgaaaac aaatcgtctt cagacggcat ttccccttca
     4081 actcactctt cacccaataa ctgctcgcgc gtcaagagga aaacaaaacc gtcgcccccg
     4141 ctggtttcca accaagtaaa aggcaactcc ggatacgctg cttccaatac atccctgtta
     4201 tgcccgattt ccaccagcaa tacacctttg ggattcagaa actttgccgc attcagaaga
     4261 atctgcctgg tggcatccaa cccgtccgcc ccgctgccca atgccaattc cggttcgtgc
     4321 aaatactctt caggcaataa ctcaaccgat tccgcatcca cataaggagg attggaaaca
     4381 atcaaatcat aagtgccttc caatccttca aacaaatccg tatgaataag ccggatgcgt
     4441 tcttccaaac cataatcttc gacattaatc cctgccactt ccaaagcatc caagctcaca
     4501 tcaaccgcat caatttgggc atcaggataa tgatgcgcca tctgaatggc aaggcaaccg
     4561 cttccggtgc aaagatccaa agcattatgc accaactcat cgtattctat ccaaggacga
     4621 agtccgtcac ccaacaattc ataaataaaa gaacgaggta tgattacgcg ctcatccaca
     4681 tagaaatcaa actctccctg ccatgcctgg tgtgtcaaat aagcggctgg aatgtgttcg
     4741 acagcacgac gctcaataac cgccagcact tcctcttttt cagcttccaa gagttttgca
     4801 tcaagatatg gggcaagcat atccaaaggc aaattcaaag tatgcagaat caaataagct
     4861 gcttcatcat gcgcattatc tgttccatga ccaaaaaaga gccctgcctc attaaaacgg
     4921 ctgactgcaa aacgtaaaat atcgcggata gtcgtcaatt cttgtgctgc ctgattaaac
     4981 ataatatgaa ccattctgcg tatagatact tttaattata acagaaacaa caagcaaacc
     5041 ttttcatatc gccaaataac cacccaatct acccatacaa ctacataaat gcccgcgcga
     5101 aaaccatcgc ccgaacggaa acgacaatgg ccgacggtat gggcaatctg attggctggg
     5161 aaaaaacggg gcttgttgtc ggtaagcagt ggataaccgc aaaagacgac aaggtgtccg
     5221 atgtctgcaa tgccaacggc gagatgggcg taatcgggct ttacgagcct ttctcacacg
     5281 gcgcattgac gatacccggt catccgaact gccgatgcga ggttgtttcc gtatcgggtg
     5341 gcgaattggg ggaatttgcc gaaaaaaagg agcttcgtaa agcggctatg cagtatgcgc
     5401 gggataactt tatcggcaaa agctatgtca ataaaaacag cgggcatgaa ctgaaggtaa
     5461 cttggcaagg tgtgaaacac gctgcgtcaa aggcaaatca ggcggaatta tccatcatga
     5521 caaaacttga tgacttattg cgctacgcaa aatatgaggg ttcttattcg gataggaaag
     5581 gtcatcctaa tattattgca gcacataagt atcgtgccgt tgccaaggtt gggaatgagt
     5641 ctttaaatat cggtgtgatt gtaagggaat ttccagacga ccataaacat tacgaccatt
     5701 tcatcttgaa ggatgaataa agcccttttg cagtgtcgtt ctggagcgga tagcgttaag
     5761 gcaagtacac ttccagcctt gaaaaagggc tttaaattca gcatgccatt tatacaggca
     5821 ggagtaaacc catgacaaag ttatacgcag aaatcgccaa gatggagacg caggacgacg
     5881 acacggtcaa ggtttggggt tacgcttcaa gcgaggaaat cgattcggac ggcgaagtca
     5941 tcgcggcggc agctatgaag gcggcgattc ccgattatat gaagtttggc gcggggcgcg
     6001 agatgcacgg ctcaaacgct gcgggaacgg caattgaaat caacgtggaa gatgacggca
     6061 gaaccttttt cgtggcgcat atcgtcgatc ccgttgccgt gacgaaggtc aaaacaggcg
     6121 tttacaaggg cttttccatc ggcggcagcg ttaccgccca cgatgagttg aacaagtcgc
     6181 aaatcacggg tttgaagctg acggaaatca gcttggttga ccgacccgcc aatcccg
//
LOCUS       DAWIVB010000125         6187 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWIVB010000125 DAWIVB010000000
VERSION     DAWIVB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.125"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(265..1308)
                     /locus_tag="WIX60_001576"
     CDS             complement(265..1308)
                     /locus_tag="WIX60_001576"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ5305825.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFVDI
                     LPTGSAPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLDAIPLLP"
     gene            complement(1305..1586)
                     /locus_tag="WIX60_001577"
                     /pseudo
     CDS             complement(1305..1586)
                     /locus_tag="WIX60_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1841..2995
                     /locus_tag="WIX60_001578"
     CDS             1841..2995
                     /locus_tag="WIX60_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ5305826.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3224..3577
                     /locus_tag="WIX60_001579"
     CDS             3224..3577
                     /locus_tag="WIX60_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305827.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3638..4354
                     /locus_tag="WIX60_001580"
     CDS             3638..4354
                     /locus_tag="WIX60_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ5305828.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            4419..4862
                     /locus_tag="WIX60_001581"
     CDS             4419..4862
                     /locus_tag="WIX60_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ5305829.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKSTITAYGEEKPLQIGMTLEADILHEKR"
     gene            complement(5111..5218)
                     /locus_tag="WIX60_001582"
                     /pseudo
     CDS             complement(5111..5218)
                     /locus_tag="WIX60_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            5228..5530
                     /locus_tag="WIX60_001583"
     CDS             5228..5530
                     /locus_tag="WIX60_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305830.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5814..6053
                     /locus_tag="WIX60_001584"
     CDS             5814..6053
                     /locus_tag="WIX60_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ5305831.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
BASE COUNT         1696 a         1359 c         1525 g         1607 t
ORIGIN      
        1 aggcgggaat ctagaaatga aaaaccacgg tgttatcggc aatgactgaa accggagaaa
       61 tgacggaatc gaagcgtgtg gaaatgtgtc ggaaattgct gaagctggat aaactagatt
      121 cccgcttttg cgggaatgac ggtattttag atttttgttt tcgtgtgaat ggatgggtca
      181 aaagccgtcc gaacccaaaa aaacgtcgct ttcagacggc atcaaaaacc gcaggcacga
      241 tgcctgcggt ttgctgtttg cgcttcaggg gagcaggggg attgcgtcca agccgtgtgt
      301 ttcctccagt ccgaacataa tgttcatatt ttggactgcc tgacccgccg cgcctttgac
      361 gaggttgtcg atgacggaaa ggacgaccca cacatcggat tgcgccgcct gttggatgct
      421 gatgcggcag aggtttgcgc cgcgcacgct gcgggtttcg ggggcggaac cggtcggcag
      481 gatgtccacg aacgggctgt cgcggtagta gtcgcgcagg acggtttcgg ggtcgctgcc
      541 gtctgaaagg tggaggtaaa cggtggcgtg cataccgcgt atcattggcg cgaggtgcgg
      601 cgtgaacacg aatccttcgg cgatgccgtc ctgaagcccg gcgatggtct gcctgatttc
      661 gggcaggtgg cggtgtccgg ctatgccgta ggctttgaag ttgtcgccgg cttcgcacaa
      721 cagcgaaccg acattgcctt tcctgcccgc gccggacaca ccggatttgc agtcggcaat
      781 cagcggcata ccgggcttca gacggcattg ccgcaacagc ggcacgagcg gtagggatac
      841 gcaggtcggg tagcagccgg ggttggcgac gaggcgcgcc tgtgcgacgg cttcgcggtt
      901 gagttcgctc aatccgtaca cggcttggga aacgaggtcg ggggcggcgt gggtcatgcc
      961 gtaccagtgt tcccaggtcg gaatgtcccg tatgcggaag tcggcggaaa ggtcgatgac
     1021 gcgcacgccc tgttcaatca ggcgcggcgc gtctttcatg gcgatgccgt tgggcgtggc
     1081 gaagaagacg atgtcgcatt gttccaaacc tgcctcgtcg ggcgtttgga aggcgaggcc
     1141 gtacacgccg cgcaaactcg gaaagtaatc ggcaactgcg gttcccgctt cgctgcggct
     1201 ggttacggcg gcgacttcga catcgggatg ggcggcaagc aggcgcagca gttccacgcc
     1261 ggtgtagccc gtcgcgccga caatgccgac tttgattttt ttactcatgg tttttccttt
     1321 gtgtggttgg cgggtatgcc gtccgaacgc tgtccgacgg ggttttgatt ggcggcggaa
     1381 ggacggtaca caaagcgttt gaaaagcgtg tctaatttaa tggcatccgc ctctgttttt
     1441 aaaacctagc cctgccgtcc ggaataaaca taaccgtagg cgcgaaagcc ggcaggatat
     1501 ttcggtgcct gccccaaacc caaaccgtat gcggcgaaag cggtggtcgt ggagaaggaa
     1561 agcaggagga ggacgagggt tttcataagg ttccaaaccg aacggggcga tgaagcccga
     1621 ttatagcaaa aggtcgtctg aaaaccgttt tcagacgacc ttttatttaa ttacaataaa
     1681 aatgttaaca acaaaaaaca aaccgctttt ttccgtttgg agacaaattt ttaactaaaa
     1741 ttgtcaacaa ttccttgacg cacacacaaa aaaaaacttt aatatttgta aatatatgta
     1801 acattataaa ttttaaatat tttctgagta aggggaagta atgagcatcc atctcgactt
     1861 cggcattagt cctaaaacgt tccgacagac ttatctgtat caaaagccca agctctttaa
     1921 aggagcggtt cggaatctcg aatccgcatc ttggaaagat atcaacgaga tataccaacg
     1981 agcagaccca accgcaccgc tgtttcatct gcgtaaaaaa ggcgcaatcg ttcctaaaga
     2041 agaatacgtc gaaagtttcg acgatttggg caaaactcgc taccgtttta ttaaatccgt
     2101 tatctacgaa catatgaaga atggtgcgtc gttagtctat aaccatatta acaacgagcc
     2161 gttttcagac catatcgccc gtcaagtcgc ccgctttgcc ggcgcacata ctattgttag
     2221 tggatatctt gcttttggca gcgacgaatc ttataaaaac cattgggacc cccgcgatgt
     2281 gtatgccatc cagcttttcg gcaagaaacg ttggcaactt actgcccctg atttccctat
     2341 gccattgtat atgcaacaga ctaaagatac tgatatttcc attcctgaac atatcgatat
     2401 ggatattatc cttgaagcag gtgatgtcct ctacatccca cgcggttggt ggcacagacc
     2461 tatcccgctc ggctgtgaaa ccttccactt cgctgtcggt acctttccac ccaacggcta
     2521 taattacctc gagtggctaa tgaagaaatt tcccaccata gaaagtctgc gccacagttt
     2581 ctcagactgg gagcaagata ggacgcgtat caacgatact gccgcacaaa ttgctgccat
     2641 gattgccgac cccgtcaatt atgaagcctt cagtgaagac tttctcggca aagaacgtac
     2701 cgataccgct tttcatctcg aacagttcgc gaatcccaac gctactccgc tttcagacga
     2761 cgtcaggttg agattaaatg ccaataattt ggatacgttg gaaaagggat atttgattgg
     2821 gaatgggatg aagataagcg tagatgagtt ggggaaaaaa gtgttagaac acatcggtaa
     2881 gaatgaaccg ttattgttga aaaatctact ggttaacttc aatcagggaa aatatgaaga
     2941 agttaggaag ttgatttatc agttgataga gttagatttt ctggaaattt tgtgagggat
     3001 tctatgaaaa actggaagca atttacattt ttcgtaatat tagtaatagc ttgttatcaa
     3061 gtgctatatt ttttatcaga tatgtttctt ctcgactaca ttaacaaata tagttggaat
     3121 ttgaatttta ttcagggtac tttgaatttt ttttcaatat atctgccata tgtttttgtt
     3181 agccgaattt ttaggaatac caaccaagaa aaggagtata aaaatgattg aacttcaact
     3241 tcatgaattg aagctggttt cagggggagg tcctgtaaca gacaatatag ctggaaatgt
     3301 agctaatgct gccacaacca aaggaggtcc cacatggggg gatttggttg caatacctgc
     3361 tgccgcagcg gcagtttatt atctgccgaa aaatgcttac ggtgctgctg gtgcaaatgg
     3421 tgtatacaat gtgactcgta attgggtaaa tgatgccgtt aatgcacctc cttataacgg
     3481 aagaccaatc tttgagattg aacatggatt aactgctccc gcaacaaaag cagataaatc
     3541 aggaaacggc tacactgacg gtacagatta ctgctgatat ttctcatcgt ccagacaatc
     3601 tctaggggtc gtctgaaact ttcttaactt caattttatg aatagaccca agcaaccctt
     3661 cttccgtccc gaagtcgccg ttgcccgcca aaccagcctg acgggtaaag tgattctgac
     3721 acgaccgttg tcattttccc tatggacgac atttgcatcg atatctgcgt tattgattat
     3781 cctgtttttg atatttggta actatacgcg aaagacaaca gtggagggac aaattttacc
     3841 tgcatcgggc gtaatcaggg tgtatgcacc ggatacgggg acaattacag cgaaattcgt
     3901 ggaagatgga gaaaaggtta aggctggcga caagctattt gcgctttcga cctcacgttt
     3961 cggcgcagga ggtagcgtgc agcagcagtt gaaaacggag gcagttttga agaaaacgtt
     4021 ggcagaacag gaactgggtc gtctgaagct gatacacggg aatgaaacgc gcagccttaa
     4081 agcaactgtc gaacgtttgg aaaaccagga actccatatt tcgcaacaga tagacggtca
     4141 gaaaaggcgc attagacttg cggaagaaat gttgcagaaa tatcgtttcc tatccgccaa
     4201 tgatgcagtg ccaaaacaag aaatgatgaa tgtcaaggca gagcttttag agcagaaagc
     4261 caaacttgat gcctaccgcc gagaagaagt cgggctgctt caggaaatcc gcacgcagaa
     4321 tctgacattg gccagcctcc cccaagcggc atgagacaga acaaagctgg cttgaacgca
     4381 ccattgccga tatttctcaa gaagttttgg attttgaaat gcgctctgaa caaatcatcc
     4441 gtgcaggacg gtcgggttat atagcaatac cgaacgtcga agtcggacag caggttgatc
     4501 cttccaaact gctcttgagc attgttcccg aacgtaccga gctatatgcc catctatata
     4561 tccccagcag tgcagcaggc tttatcaagc cgaaagacaa ggttgtccta cgttatcagg
     4621 catatcccta tcaaaaattc gggcttgctt ccggcagtgt cgtatcagta gcaaaaacgg
     4681 cactgggcag acaggaattg tcgggattgg gcatggtatc ctccgatttg gcgaagagca
     4741 acgaacctgt ttatcttgtg aaaataaaac ccgacaaatc aaccatcact gcatacggtg
     4801 aggaaaaacc gctgcaaatc ggcatgacgt tggaagcaga catcctacac gagaaacggt
     4861 gattgtacga atgggtattg gagctgattt atagtatgtc gggcaaactg taagacttga
     4921 ggctgtaaag ataaagaagt tgtctgaaac cttttcagac gaccagaaga caatatataa
     4981 aaacacaaac ttcaaaactg cctgaaacca tcccctttta caggcactcc gtttgtaaaa
     5041 gcaaggatag ggcatctgcc cttttgattg tttgattaac gatacaagga gtttcaaaat
     5101 gagagtttta tagtggatta aatttaaacc agtacagcgt tgcctcgcct tgccgtacca
     5161 tttgtactgt ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctatatgtgt
     5221 tcatgaaatg acttgggtcg gaggctcagg taatgcgcaa caaagctcat attattgcga
     5281 aatttgcgaa tctgcagggc ttaacgatac gggaaatcct gataaatctt taggattgcc
     5341 aaacaatacg ttcagtaatc cgcctggttg gggagctaca atcggagctt tagcaggtag
     5401 ccgcataggg atgcctgaat ttggtacgtt tgcgagccat gccattgaaa atttcgactg
     5461 gtcatggtat cgacgttata gggaaattgc cgaaacgatt gaacgagaat attcaggcgg
     5521 tttgccttaa tagttgagga ggtcatgatg tttgccaaac attatcaatt catcgcactc
     5581 ggcatcatgc tgcttcttta tatgttgatt ctctatacga ccgatttttc caatctgacg
     5641 tattggatgc tgttttttat ctgttttatt acaggaaaaa tattagctcg tttgttagag
     5701 aaaagcttta aataaaatag cagctagtcg caaaaggtcg tctgaaacct tttcaggcgg
     5761 cctttctaaa atacatccaa cttcctaatc cctatttttc aaaaaggaaa tctatgcccc
     5821 atctgcaaaa cctgtctttg ggcttaaaga aaaagctgcc tgttatcctg caaacagaaa
     5881 tatcagaatg cggcttggca tgtctggcgg ctgtggcggg atttcatggt ttccatacga
     5941 atttacgcgc actgcgttca aaatactgtc cgagaccttt gcaaaattcc ccaaaatccc
     6001 ctaaatgtct tggtgggaat tttggggaat tttgcaaagg tctcattcta taactgtaaa
     6061 tacttttaaa tttatgacaa aatagtaaat attgctaaaa taatattgat gtcatgaaat
     6121 tttttcctgc tccatgtctg ttggttatcc tggctgtcat accccttaaa accttagctg
     6181 ccgatga
//
LOCUS       DAWIVB010000126         6134 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWIVB010000126 DAWIVB010000000
VERSION     DAWIVB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.126"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            106..2247
                     /locus_tag="WIX60_001585"
     CDS             106..2247
                     /locus_tag="WIX60_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ5305832.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNWYPRAEESEVKLNESDWEATGLPTDPKELPK
                     RQKSVIEQVKTDDNSNIYSSPYLTPSNHQNGNAGNATNQPKNEVKDYKEFKYVYSGWF
                     YKHAKQDIDSKNKIVRQGDDGYIFYHGKEPSRQLPASGKVTYKGVWHFVTDTKKGQEF
                     REIIQPSKKQGDRYSGFSGDDNEEYSNKKETILQNGHEGYGFTSNLEVDFGNKKLTGK
                     LIRNNASLNNNTNNDKHTTQYYSLEAQVTGNRFSGTATATDKKENEAKQHPFVSDSSS
                     LSGGFFGPQGEELVFRFLSNDQKVAVVGSAKTKDNTANGNTAAASGGTDAAASNSAAG
                     TSSKNSKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPKDSESGGNQAD
                     KGKNGGTDFTYKTTYTPESDKKDTQAQTVTNSTQTASNTAGDTNGKTKTYEVEVCCSN
                     LNYLKYGMLTRKTAGNTGESGNSSPTAAQTDAQSMFLQGERTDEKEIPKEQQDIVYRG
                     SWYGHIANDTSTSWSGNASNATSGNRADFTVNFADKKITGKLTAENRQEATFTIDGKI
                     EGNGFSGTAKTDNLGFDLDQSNTTGTPKAYITDAKVQGGFYGPKAEELGGWFAYSDDK
                     QTKNATDASGNGNSASSATVVFGAKRQQPVR"
     gene            2334..5087
                     /gene="tbpA"
                     /locus_tag="WIX60_001586"
     CDS             2334..5087
                     /gene="tbpA"
                     /locus_tag="WIX60_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ5305833.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGVSQIQSYTAQAALGGTKTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDGHQYAHFVVEEECESRNYETCKSKP
                     KKDIVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDANKKQAGSLSGNGKYAGNHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWVDYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYHQSANRAYSSNTPPQSGNGGSNANPNGSKDKPYWVSIGGGNVVTGQICRL
                     GNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHR
                     TLSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEA
                     GIVFKGDFGNLEASWFNNAYRDLIVRGYEAQIKDGKEEAKGDPAYLNAQSARITGINI
                     LGKIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLG
                     YDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYY
                     TVKKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLE
                     MKF"
BASE COUNT         1775 a         1540 c         1532 g         1287 t
ORIGIN      
        1 acgggatttt ggtgtattcc ctaaaaaaat aaaaaagtat ttgcaaattt gttaaaaata
       61 aataaaataa taatccttat cattctttaa ttgaattggg tttatatgaa caatccattg
      121 gtaaatcagg ctgctatggt gctgcctgtg tttttgttga gtgcttgttt gggcggaggc
      181 ggcagtttcg atcttgattc tgtcgatacc gaagccccgc gtcccgcgcc aaagtatcaa
      241 gatgtttctt ccgaaaaacc gcaagcccaa aaagaccaag gcggatacgg ttttgcgatg
      301 aggttgaaac ggaggaattg gtatccgagg gcagaagaaa gcgaggttaa actgaacgag
      361 agtgattggg aggcgacagg attgccgaca gaccccaagg aactccctaa acggcaaaaa
      421 tcggttattg aacaagtaaa aacagacgac aacagcaata tttattcttc cccttatctc
      481 acaccatcaa accatcaaaa cggcaacgct ggcaacgcta caaaccaacc aaaaaatgaa
      541 gtaaaagatt ataaagagtt caagtatgtt tattccggtt ggttttacaa acacgctaaa
      601 caagatattg attcgaaaaa taaaattgta cgacaaggcg acgacggtta tatcttttat
      661 cacggcaaag aaccttcccg acaacttcct gcttctggaa aagttaccta taaaggtgtg
      721 tggcattttg taaccgatac aaaaaagggt caagagtttc gtgaaattat ccagccttca
      781 aaaaaacaag gcgacaggta tagcggattt tcgggtgatg acaacgaaga atattccaac
      841 aaaaaggaaa cgattttaca aaacggtcat gaaggttatg gttttacctc gaatttagaa
      901 gtggatttcg gcaataagaa attgacgggt aaattaatac gcaataatgc gagcctaaat
      961 aataatacta ataatgacaa gcacaccacc caatactaca gccttgaggc tcaagtaaca
     1021 ggcaaccgct tcagcggcac ggcaacggca actgacaaaa aagagaatga agccaaacaa
     1081 catccttttg tttccgactc gtcttcttta agcggcggct ttttcggccc gcagggtgag
     1141 gaattggttt tccgcttttt gagcaacgat caaaaagttg ccgttgtcgg cagcgcgaaa
     1201 accaaagaca acaccgcaaa tggcaatact gcggcggctt caggcggcac agatgcggca
     1261 gcatcaaaca gtgcggcagg cacgtcgtct aaaaacagta agctgaccac ggttttggat
     1321 gcggtcgagc tgacgcacgg cggcacagca atcaaaaatc tcgacaactt cagcaacgcc
     1381 gcccaactgg ttgtggacgg cattatgatt ccgctcctgc ccaaggattc cgaaagcggg
     1441 ggcaatcagg cagataaagg taaaaacggc ggaacagact ttacctacaa aacaacctac
     1501 acgccggaaa gtgataaaaa agacacccaa gcccaaacag tcacgaacag cacgcaaacc
     1561 gcttcaaata cggcaggtga taccaatggc aaaacaaaaa cctatgaagt cgaagtctgc
     1621 tgttccaacc tcaattatct gaaatacgga atgttgacgc gcaaaactgc cggcaatacg
     1681 ggggaaagcg gcaacagcag cccaaccgcc gcccaaacgg acgcgcagag tatgttctta
     1741 caaggcgagc gcaccgatga aaaagagatt ccaaaagagc aacaagacat cgtttatcgg
     1801 gggtcttggt acgggcatat tgccaacgac acaagcacaa gctggagcgg caatgcttcc
     1861 aatgcaacga gtggcaacag ggcggacttt accgtgaatt ttgccgataa aaaaattacc
     1921 ggcaagttaa ccgctgaaaa caggcaggag gcaaccttta ccattgatgg taagattgag
     1981 ggcaacggtt tttccggtac ggcgaaaact gataacttag gttttgatct cgatcaaagc
     2041 aataccaccg gcacacctaa ggcatatatc acagatgcca aggtgcaggg cggtttttac
     2101 gggcccaaag ccgaagagtt gggcggatgg tttgcctatt cggacgataa acaaacgaaa
     2161 aatgcaacag atgcatccgg caatggaaat tcagcaagca gtgcaactgt cgtattcggt
     2221 gcgaaacgcc aacagcctgt gcgataagca cggttgccga acaatcaaga ataaggcttc
     2281 agacggcacc gctccttccg ataccgtctg aaagcgaaga ttagggaaac attatgcaac
     2341 agcaacattt gttccgatta aatattttat gcctgtcgct gatgaccgcg ctgcccgttt
     2401 atgcagaaaa tgtgcaagcc ggacaagcac aggaaaaaca gttggatacc atacaggtaa
     2461 aagccaaaaa acagaaaacc cgccgcgata acgaagtaac cggtttgggc aaattggtca
     2521 aaaccgccga caccctcagc aaggaacagg tactcgatat ccgcgacctg acgcgttacg
     2581 accccggcat cgccgtggtc gaacaggggc gcggcgcaag ctcgggctat tcgatacgcg
     2641 gtatggacaa aaaccgcgtt tccttaacgg tagacggcgt ttcgcaaata cagtcctaca
     2701 ccgcgcaggc ggcattgggc ggaacgaaga cggcgggcag cagcggcgca atcaatgaaa
     2761 ttgagtatga aaacgtcaaa gctgtcgaaa tcagcaaagg ctcaaactcg gtcgaacaag
     2821 gcagcggcgc attggcgggc tcggtcgcat ttcaaaccaa aaccgccgac gatgttatcg
     2881 gggaaggcag gcagtggggg attcagagta aaaccgccta ttccggcaaa aaccgggggc
     2941 ttacccaatc catcgcgctg gcggggcgca tcggcggtgc ggaggctttg ctgatccaca
     3001 cgggtcggcg cgcgggggaa atccgcgccc acgaagatgc aggacgcggc gttcagagct
     3061 ttaacaggct ggtgccggtt gaagacggcc accaatacgc tcatttcgtg gtcgaagaag
     3121 aatgcgaaag cagaaattac gaaacgtgta aaagcaaacc gaaaaaagat attgtcggca
     3181 aagacgaacg tcaaacggtt tccacccgag actacacggg tcccaaccgc tttctcgccg
     3241 atccgctttc atacgaaagc cgatcgtggc tgttccgccc gggttttcgt tttgaaaaca
     3301 aacggcacta catcggcggc atactcgaac acacgcaaca aactttcgac acgcgcgata
     3361 tgacggttcc ggcattcctg accaaggcgg tttttgatgc aaataaaaaa caggcgggtt
     3421 ctttgtccgg caacggcaaa tacgcgggca accacaaata cggcggactg tttaccaacg
     3481 gcgaaaacgg tgcgctggtg ggcgcggaat acggtacggg cgtgttttac gacgagacgc
     3541 acaccaaaag ccgctacggt ttggaatatg tctataccaa tgccgataaa gacacttggg
     3601 tggattatgc ccgcctctct tacgaccggc agggcatcgg tttggacaat cattttcagc
     3661 agacgcactg ttctgccgac ggttcggaca aatattgccg cccgagtgcc gacaagccgt
     3721 tttcctacta caaatccgac cgcgtgattt acggggaaag ccacaggctc ttgcaggcgg
     3781 cattcaaaaa atccttcgat accgccaaaa tccgccacaa cctgagcgtg aatctcgggt
     3841 ttgaccgctt tggctctaat ctccgccatc aggattatta tcatcaaagt gccaaccgcg
     3901 cctattcgtc gaacacgccc cctcaaagcg gcaacggcgg ctcaaacgcc aaccccaacg
     3961 gcagcaagga caaaccctat tgggtcagca taggcggggg aaatgtggtt acggggcaaa
     4021 tctgccgctt gggcaacaat acttatacgg actgcacgcc gcgcagcatc aacggcaaaa
     4081 gctattacgc ggcagttcgg gacaatgtcc gtttgggcag gtgggcggat gtcggcgcgg
     4141 ggttgcgcta cgactaccgc agcacgcatt cggacgacgg cagcgtttcc accggcacgc
     4201 accgcaccct gtcctggaac gcaggcatcg tcctcaaacc tgccgactgg ctggatttga
     4261 cttaccgcac ttcaaccggc ttccgcctgc cctcgtttgc ggaaatgtac ggctggcggg
     4321 cgggtgttca aagcaaggcg gtcaaaatcg atccggaaaa atcgttcaac aaagaagccg
     4381 gcatcgtgtt taaaggcgat ttcggcaact tggaggcaag ttggttcaac aatgcctacc
     4441 gcgatttgat tgtccggggt tatgaagcgc aaattaaaga cggcaaagaa gaagccaaag
     4501 gcgacccggc ttacctcaat gcccaaagcg cgcggattac cggcatcaat attttgggca
     4561 aaatcgattg gaacggcgta tgggataaat tgcccgaagg ttggtattct acatttgcct
     4621 ataatcgtgt ccgtgtccgc gacatcaaaa aacgcgcaga ccgcaccgat attcaatcac
     4681 atctgtttga tgccatccaa ccctcgcgct atgtcgtcgg cttgggctat gaccaaccgg
     4741 aaggcaaatg gggtgtgaac ggtatgctga cttattccaa agccaaggaa atcacagagt
     4801 tgttgggcag ccgggctttg ctcaacggca acagccgcaa tacaaaagcc accgcgcgcc
     4861 gtacccgccc ttggtatatt gtggacgtgt ccggttatta cacggttaaa aaacacttta
     4921 ccctccgtgc gggcgtgtac aacctcctca actaccgcta tgttacttgg gaaaatgtgc
     4981 ggcaaactgc cggcggcgca gtcaaccaac acaaaaatgt cggcgtttac aaccgatatg
     5041 ccgcccccgg tcgcaactac acatttagct tggaaatgaa gttctaaacg tccaaacgcc
     5101 gcaaatgccg tctgaaaggc ttcagacggc attttttaca caatccccgc cattttccat
     5161 catccccgat acaccgtaat ctcgaaaccc gtcattcccg cgcaggcggg aatccagtcc
     5221 gttcggtttc ggtttttttg aggtttcggg taacttctaa accgttattc ccgcgaaaac
     5281 agaaaatcaa aaacagaaac ctcaaatccc gttattcccg agcagacggg atctagggcg
     5341 taaaatctaa agaaaccgtt ttatccgata agtttccgca ccgacagact agattcccgc
     5401 ctgcgcggga atgacgttat atttttcgca tttgataaaa aagaccgttt gaaatttttt
     5461 cagcggacgc aaagtattgc gtaaaatgct gcttataaga aacgcaaggc gggcgtagga
     5521 tgtgcagcgt ggacataggg ctggctgtag ggtgggcttc agcccaccaa tcccaccgtt
     5581 tccacctatt cccccaactc cgtcaatgtt atccattccg cccattccca ccgaaaaccg
     5641 aaaccgccgt attcccaaaa acctttgatg cggtgaaatt ggtgggctga agcccaccct
     5701 acagcccacc ctacggctcg ccgaaatttc gtcattcccg cgcaggcggg aatccaggtc
     5761 tgtcggtgcg gaaacttatc ggataaaacg gtttcttgag attttacgtc ctagattccc
     5821 acttccgtgg gaatgacggg atgcaggttt tcgtgcggac gcgttcggat tcacgactgc
     5881 gcgggaatga cgggatttca gtttgcggat tgatttgaag ttgcaaaatc ccaacggatc
     5941 ggattaccgc tttcgcgttt caaagttacg gcgttatcgg aaaaacagaa aatcaaagct
     6001 gcaagaattt atttaaaaca accgaatttc aacggatcgg attctcgcct gtagggaatg
     6061 acggcggaag gttttttgtc ttttctgaca gatgtccgca atctgaaatc ctgaccgtgg
     6121 gaacgacggt atag
//
LOCUS       DAWIVB010000127         6128 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWIVB010000127 DAWIVB010000000
VERSION     DAWIVB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.127"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(55..1566)
                     /gene="ubiB"
                     /locus_tag="WIX60_001587"
     CDS             complement(55..1566)
                     /gene="ubiB"
                     /locus_tag="WIX60_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ5305834.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGEQVAVKVLRPNLLPVIEQ
                     DLSLMRFGAGWVERLFADGKRLKPREVVAEFDKYLHDELDLMREAANASQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(1601..2065)
                     /locus_tag="WIX60_001588"
     CDS             complement(1601..2065)
                     /locus_tag="WIX60_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ5305835.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPSRNIKLLVFGLLIAFLGYLLGKIF"
     gene            complement(2062..2208)
                     /locus_tag="WIX60_001589"
     CDS             complement(2062..2208)
                     /locus_tag="WIX60_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305836.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(2225..2563)
                     /gene="erpA"
                     /locus_tag="WIX60_001590"
     CDS             complement(2225..2563)
                     /gene="erpA"
                     /locus_tag="WIX60_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ5305837.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(2695..3381)
                     /locus_tag="WIX60_001591"
     CDS             complement(2695..3381)
                     /locus_tag="WIX60_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ5305838.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3569..3841
                     /locus_tag="WIX60_001592"
     CDS             3569..3841
                     /locus_tag="WIX60_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305839.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            4049..5977
                     /gene="dnaK"
                     /locus_tag="WIX60_001593"
     CDS             4049..5977
                     /gene="dnaK"
                     /locus_tag="WIX60_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ5305840.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLITRSIEPCKIALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1757 a         1648 c         1344 g         1379 t
ORIGIN      
        1 ctataaccga tttccttata aacgggcgga gtcgggcgat gattcggaca aagcctattt
       61 caaaagcaaa atcagcagca aaacaaccgc aatcacagcc agccacaggc tttgccgctg
      121 ctgcactttg accaaatgga tataggcatc acgcatttcc tgctggcggt tttcatcaat
      181 caacgcactg attttgcgcg gcaatgaagg gatgatttgc gcccagtcgg gggcttcgtt
      241 tttgaggttg cgccaaaggg ctttagggcc gacctgcccg ttcatccatt tcaccaaaaa
      301 cggtttggcg gttttccaca agtccaaatc gggatcaagc tgccgtccca agccttcgat
      361 gttgagcagc gttttttgca gcaataccag ctgcggctgg atttcgacat tgaagcggcg
      421 gctgacttca aacaggcgca tcagcaccaa gccgaaggaa atctgcgaaa tcggtttgtt
      481 gaacactggt tcgcacacgg cgcggacagc cgcttccaac tcttccgcgc gcgtgtcggc
      541 gggcacccag cccgattcga tgtgggcggt ggcgacgcgc cggtaatcgc ggttgaagaa
      601 ggcgaggaag ttgatggcga gataacgttt gtcgtaatcg gtcagcgtgc cgacgatgcc
      661 gaaatcgagg gcgatgtagc ggttgtcggc ggcaaccaaa atattgccgg ggtgcatatc
      721 cgcgtggaaa aagccgtcgc ggaagacttg cgtgaagaag atttccacgc cgtaatcggc
      781 gagtttgtgc aaatcgatgc cgtctgcttt gagtttggcg atgtcggaaa ccggcgtgcc
      841 gtccatccat tcgatggtca gcacgtcgct ggtgcagtag tcgtaaaaca ccttcggcac
      901 aatcagcata tcgctgtttt ggaaattgcg tccgagctgg ctggcattgg cggcttcgcg
      961 catcaagtcc aactcgtcgt gcagatattt gtcgaactcc gccaccactt cgcgcggctt
     1021 cagacgcttg ccgtcggcaa acagacgctc gacccagcct gcaccaaagc gcatcagcga
     1081 caaatcctgt tcgatcacgg gcaaaaggtt ggggcgcaaa actttaaccg ccacttgttc
     1141 gcccgaatgc aggcgggctt tgtgtacttg ggcgatggac gcgctggcga tgggctcggt
     1201 ttcaaattcc gcatacagct tttcgatgga ctgacccaac gatttttcga tttgttcacg
     1261 cgaaagccgc gcgtcaaaag gcggcacttt gtcttgcagc tttgccagtt cgacggcgta
     1321 atcgtgcgga atcaaatcgg ggcgtgtgga caaaacctgc ccgaacttga tgaaaatcgg
     1381 ccccaggctt tccaaggcaa ggcgcagacg gacagcaggc ggttcgtttt tcaatttgga
     1441 cgactgcggc atcattttca gcagagcgca tatccaaccg ctacccatca gcgaagcaca
     1501 cagacctgcc agccgatagc ggtaaaaagt cccgacaata accgtcaggc gtttcaacca
     1561 tttcatatat aaaatcccga atacagcatt tatcggacaa tcaaaaaatc ttaccgagca
     1621 gatagcccaa aaaggcaatc agcagcccga aaaccagcag tttgatattg cgggacggcg
     1681 gttcgggttg cgaaggtgcg gaaatatcat cttcccgccc gccgtgaagt tcaagatatt
     1741 cgggcatttc cccatccgcg ccttctcctt cgccctcccc ttcctccggc ggcacgaccg
     1801 tttccaactc catatatctc cgggtaataa tatccaaacc caaatagctg aaataatcat
     1861 gcaactcttc ccgtttcgcc caagtgcatt tttcctcaac cagcacttga ggtttggaat
     1921 cgagcgattc gatcaatcct tccgcctttt cctcgccgag attctcctta aggcactccc
     1981 ggataagccc gcgatccact tggggcgggt aagaaacata tacatcgtac aaatcgtttc
     2041 ggtaatcttt actcatctca atcataaggg aacaaatacg cctgataccg ttccgcccgt
     2101 tcctttcggg aaacccgatt catcttcaaa taatcggatt atatgtgaat tcatcccgat
     2161 aatatatttt ctaaaagtaa aaatgccgtc tgaacgttca gacggcatca cacaaaccaa
     2221 gcggtcatac ggaaaacgac gatccgcaac cgcaggttgt ttccgcattc ggattgcgga
     2281 tgacgaattg cgaaccctgc aaactttccg tatagtcgat ttccgcaccg accagatatt
     2341 gatagctcat cggatcgacc aaaaagacca aaccgttttt ctcaatttca aaatcgtcgt
     2401 cgtttttgat ttcgtcaaaa gtaaatccgt actggaaacc cgaacagccg ccgccattga
     2461 caaaaacccg caatttcaaa tcgggattgt tttcttcggc aatcaaatcg gcaactttgg
     2521 cacagcagct gtcagtaaaa ataatagggc tttcatccga catggtgtta ttccttaata
     2581 taatattttc tgcattctct ctcaaacccc cttcaaaggc aagcaggctt cgcctttgaa
     2641 aaaggaattg ttccaataga taagtttgac cgcctgcaat ttcaagcccc aaactcaaat
     2701 cgtcctgccg aaccactcga cacgcccgat aatctccaca tcatcggtca aatcgttcaa
     2761 attgatttca aaagcaggat aagcctcgtt tgcagaaatc acattgataa tcccgcccgg
     2821 tacaatctgt aaacgtttaa ccagcagatt ttcattaatc cgcaacacat acagaccgtc
     2881 cctcggcgta ttttcaccat gattgaccaa aatcgaatcg ccgtcattca aaaccccctc
     2941 catcgaatcc cccttgacgg aaattacaga caggtttttc gtatcgcggg taacataatt
     3001 ctcaatccaa tgccgtctga acgccattgt aaataccggt tcctcatgat cgacaaactg
     3061 cccgtatccc gcagccgccc gaatatcata tctcggcacg aagacaaact cgtccgtatc
     3121 gacttcattg cccaaagtat cgtaagcaag ggattttttt ggggcttcat ccggaaacgg
     3181 attaccctcc ccggtcagca gccaatcgat actacacccc ttcaactgct tgattttttt
     3241 caatgtttca gactttggca gaccgccttc attccatatc ctgctgaagc ccgcaatcgt
     3301 catttcaata tcggatgcga tttttgcctg cctcgcttcg cttttccata aaaaaaccag
     3361 tctgtcttta aaggtttcca taaatatcct tccactcccc ttcattcaat catctaaagt
     3421 aaacatgaaa tatattttac cttagctatc caaaaaacgg aacaattatg ataaactaaa
     3481 tttaaattat gatttttctt acttaataca taatttttca gatttttcca taggtttaat
     3541 cctgaattga cagaaaaagg atagaaaaat gaagaaaacc ggcaaatatc ttatctatac
     3601 tgcggcattt accgcattct gctttgcctt ccaagaaaac cgttctgaag ccaaacagcc
     3661 cgacatcact ttatccgcat ccctgtgcga acaattcaac atactgaacg ccaaagatat
     3721 ggatgcagaa caagtctccc tttccaaaga atgcgacatc atcgagtctt cacacgactg
     3781 ggaaaaagag tacggcaact tgaacgaaca ggaaatgctc gccggcatcg tctatgaata
     3841 aacctgcctg ccatttgaaa cattatgctt gaatgcattg gagccaaatg tattaaatca
     3901 aatataaaac caatatattc ataaagttat atacttatag ccatgctgta gcttgaaaca
     3961 gcccgccgcc cgccctattt acagcccatc gggacaaaat gttccaacat atttctcaaa
     4021 ataagcaaaa tcaaatagga ttatccacat ggcaaaagta atcggtatcg acttaggtac
     4081 aaccaactct tgtttggcca tttccgaaaa cggtcaaacc aaagtgatcg aaaacgcaga
     4141 aggcgcacgc accacgccgt ccgttatcgc ttatttggac ggcggcgaaa tcctcgtcgg
     4201 tgcgcctgcc aaacgccaag cggtaaccaa cgccaaaaac actatttacg ccgccaaacg
     4261 tttgatcggc cacaaatttg aagacaaaga agtccaacgc gacatcgaat ctatgccttt
     4321 cgaaatcatc aaagccaaca acggcgacgc atgggtaaaa gcacaaggca aagagctgtc
     4381 tcctcctcaa atttccgcag aagtcctgcg taaaatgaaa gaagccgccg aagcttactt
     4441 gggcgaaaaa gtaaccgaag ccgtgattac cgtccctgcc tacttcaacg acagccaacg
     4501 tcaagccacc aaagacgcag gccgtatcgc cggtttggac gtgaaacgca tcatcaacga
     4561 gccgaccgca gccgctttgg cattcggtat ggacaaaggc gacaacaaag accgcaaagt
     4621 agccgtatat gacttgggcg gcggtacttt cgatatttcc atcatcgaaa tcgccaacct
     4681 cgacggcgac aaacaattcg aagtattggc aaccaacggc gataccttct tgggcggtga
     4741 agacttcgac caacgcctca tcgaccacat catcgccgag ttcaaaaaag aacaaggcat
     4801 tgatttgaaa caagacgtga tggctctaca acgcctgaaa gaagctgccg aaaaagccaa
     4861 aatcgaattg tccagcggcc agcaaaccga aattaacctg ccgtacatca ccatggacgc
     4921 aaccggcccg aaacacttgg cgatgaaaat tacccgcgcc aaattcgaaa gcctggttga
     4981 agacctgatt acccgctcta tcgaaccttg caaaattgca ttgaaagatg ccggcttgag
     5041 caccggcgac atcgacgacg taatcttggt cggcgggcag tcccgtatgc cgaaagtaca
     5101 agaagccgtt aaagccttct tcggcaaaga accgcgcaaa gacgtgaacc ctgacgaagc
     5161 cgttgccgta ggcgcagcga tccaaggcga agtattgagc ggcggccgca gcgacgtatt
     5221 gctactggac gtaactcctc tgtctttggg tatcgaaacc atgggcggcg tgatgaccaa
     5281 actgattcag aagaacacca ccatcccgac caaagcgtcg caagtgttct ctaccgccga
     5341 agacaaccaa agcgcagtaa ccatccacgt actgcaaggc gaacgcgaac gcgcttctgc
     5401 caacaaatct ttgggtcagt tcaacttggg cgacatcgca cctgcaccgc gcggtatgcc
     5461 gcaaatcgaa gtaaccttcg acatcgacgc caacggcatc ctgcacgttt ccgccaaaga
     5521 caaaggcacc ggtaaagcag ccaacatcac catccaaggt tcttcaggtt tgagcgaaga
     5581 agaaatcgaa cgcatggtga aagatgccga agccaatgcc gaggaagata aaaaactgac
     5641 tgaattggtc gcttcccgca accaagccga agccctgatt cactctgtga aaaaatcttt
     5701 ggccgactac ggcgacaaac tcgacgcagc cgagaaagaa aaaatcgaag ccgcgctgaa
     5761 agaagccgaa gaagcagtta aaggcgacga caaagccgct atcgatgcca aaaccgaggc
     5821 gctgggcgca gccagccaaa aactggggga aatggtttac gctcaagcac aagctgaagc
     5881 ccaagcaggc gaaagcgaac aagccaatgc ttctgcaaag aaagacgatg atgtcgtaga
     5941 tgccgacttt gaagaagtaa aagacgacaa aaaataatta ataccgtctg aaaaaagcgc
     6001 gaaccgtttg attcgcgctt ttttcaattg agataaaaga ccatagcata acagaggttc
     6061 caagttcatc taccgtaata ttcccaaacc caagttccaa ctgttgtatc ggttctctgg
     6121 aaattttt
//
LOCUS       DAWIVB010000128         6119 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWIVB010000128 DAWIVB010000000
VERSION     DAWIVB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.128"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            143..1540
                     /gene="aspA"
                     /locus_tag="WIX60_001594"
     CDS             143..1540
                     /gene="aspA"
                     /locus_tag="WIX60_001594"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ5305841.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLVVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1612..2487)
                     /locus_tag="WIX60_001595"
     CDS             complement(1612..2487)
                     /locus_tag="WIX60_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ5305842.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2489..3181)
                     /locus_tag="WIX60_001596"
     CDS             complement(2489..3181)
                     /locus_tag="WIX60_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ5305843.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYFYRIIAGFIRF
                     NGGRAVAPEKWI"
     gene            complement(3204..3683)
                     /gene="ybaK"
                     /locus_tag="WIX60_001597"
     CDS             complement(3204..3683)
                     /gene="ybaK"
                     /locus_tag="WIX60_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ5305844.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3758..4120
                     /locus_tag="WIX60_001598"
     CDS             3758..4120
                     /locus_tag="WIX60_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ5305845.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            4155..5018
                     /locus_tag="WIX60_001599"
     CDS             4155..5018
                     /locus_tag="WIX60_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ5305846.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKTLTVPNGGIFKA
                     VHLFFKTENAE"
     gene            5111..6070
                     /gene="ttcA"
                     /locus_tag="WIX60_001600"
     CDS             5111..6070
                     /gene="ttcA"
                     /locus_tag="WIX60_001600"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ5305847.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGS"
BASE COUNT         1557 a         1775 c         1477 g         1310 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      181 cgagattccc gccgaagtat attggggcat ccacaccctg cgcgccattg aaaactttaa
      241 aatctccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      301 aaaggcgacg gcacaggcaa acggtaagtt gggcgcggtc aagcccgaaa tcgcagccgc
      361 cattgaaaaa gcctgcgacg aagttctgct gaacaaccgc tgcctcgacc aattcccctc
      421 cgacgtgtat cagggcggtg cgggtacttc ggtcaatatg aacaccaacg aagtcatcgc
      481 caacctcgca ttggaagcct tgggctatga gaaaggccgc tacgacatcg tcaatccgat
      541 ggaccatgtc aacgccagcc aatcgaccaa cgatgcctat cccacgggct tccgccttgc
      601 cgtgtattac agtatcggcg aattgctcga caaactggtc gttctgaaaa acgcctttgc
      661 cgccaaagcc gaagaattta aagacgtttt gaaaatgggc cgcacccagc ttcaagatgc
      721 cgtgccaatg actacaggac aagaattcca atctttccaa gtgttgttgg aagaagaaat
      781 cctcaacctc gaccgcacgc gccaactgct tttagaagtc aatttgggcg caacggcaat
      841 cggcacgggc gtgaacacgc ccaaaggcta cgccgaactg gtggtcaaaa aactctccga
      901 agtcagcggc ttgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      961 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
     1021 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1081 cgaattacag gccggttctt ccattatgcc tgccaaagtc aatcccgtga ttcccgaagt
     1141 ggtcaaccaa gtctgcttca aagtcatcgg caacgatacg acgattacct tcgccgccga
     1201 agccgggcaa ctgcaattaa acgtgatgga gccggtcatc gcccaatgta tgtttgaaac
     1261 catttccctc ttgggcaatg cagcaatcaa cctgtccgac aaatgcgtca aaggcattac
     1321 ggtcaaccgc gaaatatgcg agcgttacgt tttcaactcc atcgggctgg ttacttatct
     1381 gaatccgtat atcggacacc acaacggcga tttggtcggc aaaatctgcg cccaaaccgg
     1441 caaaggcgtg cgcgaggttg tcttggaacg cgggctgttg agcgaagaag aactcaaccg
     1501 catcctctct cctgaaaacc tgatgaaccc ccatctgtaa cgttgcagac aaatgccgtc
     1561 tgaagcaggg atgctgcttc agacggcatt ttgttatatc aatcagccgg attatatgcc
     1621 gaacagtacc ttgacttccc cccaataaaa caggcaggcg atagaacaca gtgccaacac
     1681 gattcccgcc gtattgattg tgttgatttt ttccttaaag aacaatgcgc ccgaaagcgt
     1741 acccaaaaca atcacgccga tattcatacc ggcaaaaacc aaggtcggat tatccttcat
     1801 catttggtgc gcggtgatgt aggttacgat attcataaaa ttcaaaccgc ccaagaatat
     1861 gccgccaacc acactctcaa cacgccatct gaccgatttg gcaaacaggc aggcaaacat
     1921 cagcacaccc gccagcacaa atgcaaccag caggttgccc gcaaatgccg ttccgctttt
     1981 ggcaagctgt ttgaacagga tatcgataat gccgtaacct gcccacacgc ccagcagcaa
     2041 tgccgcctgc cgccacgcgc ttcctgattt tttgccaccg ctgtgtttcc aaagcaggca
     2101 gaacagtgcg gcaaatgcga ggcacagccc gattagtctg ccttcgctga gtttttcgcc
     2161 aaacagcgtc aaggcggcaa caatcggcaa aaacagcgac aaacgctgcg ccgcgtcgga
     2221 tttgacgata ccggcggatt ctacggattt gcccataatg acaaatacgg acggcagcag
     2281 cacgcccaat gcggcaaaca gccaccatgt cggcaaaaat gcgccgatat tgccgacatc
     2341 cggcttcaat accagcaggg tcagtatgac cgcgaccaca taattgacgg cgaccgcctg
     2401 cgcgatgtcg atttttttct tccttgccat tttcagcaaa atggaaaccg acacgctgaa
     2461 cacgatgctg attaacaggt aagccatact atatccattt ctcgggtgca accgccctgc
     2521 cgccgttgaa gcggataaag ccggcgatga tgcggtagaa ataccagacg ttcgttgcaa
     2581 taataatcag cacgccgata cccaaaaggg cagtcagcgc acccaaaata taaagccaaa
     2641 atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc ggcatagaca atgctgtcca
     2701 aactgttccg tttcatatag gcgagaatca cgccgactat gggcatgaac agcatgattg
     2761 cacccaaacc gaacaggatg taggcggcga ggacatagtt gcgcccgctt ccgccgccgg
     2821 acggttcgta agtatggcac gaacgtgatg cctgccgttc aaacgcctgt ctgaacgcct
     2881 gttcgcggcg catctgttcg cggtaaaact gctccctgcg cgcctgttcg cggcggaatg
     2941 cctcttcctg ccgcccgcgt tcctcatgcc gtctgaagga cgcgtcgtat tgcatccgtt
     3001 tcgacaggtc ggaaagcgta tcgtaagccc gttggatttc tttgaacttt tcttccgcct
     3061 tcggattgcc cggattgcgg tcgggatgat atttcatcgc cagcttgcgg taggcgcgtt
     3121 tgatttcgtc cgctcccgcc tgcggcgaca cgcccaatac ggcatacaaa tctttgtcca
     3181 tattgttcct ttatactttc ccgtcaaacc gccgcctgta ttgttttcgg gttcaaaata
     3241 tttaaatctc cgggacggat gccgataatg aacccgcgtt ttccgccgtt gatatagatg
     3301 gtttccaaat ccatcaccga ctgttcgacg taaatatcca actttgtccg gatgccgaac
     3361 ggcgttgtgc cgccgaccag atagcccgtc cacttgtttg cctgtacggg cgtggcgggt
     3421 tcaatgtgtt tcgcacccaa atgccgcgcc agattgcggg ttgaaatctg cttgtcgccg
     3481 tgcatcaaga caatcagccc cttaccgttt tcatcttgca aaacaatggt tttaatgacc
     3541 aagtgttcgt ctttgccgaa tagtcgggca aactgcgccg tgccgccgtg ttcctcatag
     3601 gcataggtaa aaggttcaaa ttcgatgccg ttttcacgca aaacgcgcac ggcaggtgtt
     3661 atcggataag tgtgtttact catttttcgt catgcaaaac atggaaatcg gtcaagcgca
     3721 atgttaccat atatcccctt tatcgaagga aaccgatatg gatatccgat atttcggcac
     3781 aacgccccgc tattccgaag ccgtcggcgc aaacggcttg attttcctct ccggcatggt
     3841 tcccgaaaac ggtgaaacgg ctgccgaaca gactgccgac gtgcttgccc aaatcgaccg
     3901 ctggctggcg gaatgcggct cggacaaagc gcacgttttg gatgcggtca tctatctgcg
     3961 cgatatgggc gactatgcgg aaatgaacgg cgtgtgggac gcttgggttg ccgccggcag
     4021 aacgcccgcc cgcgcctgcg tggaagcacg gctcgcccgt ccggagtggc gcgtcgaaat
     4081 caaaataacc gccgtgaaga gagacgcggc aactgcataa acagatttcc tttatatttc
     4141 agagtacgca aatgatgaaa accttactgc cctttttcgg cgtggtgcgc gtcagcggcg
     4201 aagaccgcca aaccttcctg cacggacagc tttccaacga catcaaccac cttcagaccg
     4261 ggcaggcgtg ttatgcgact tacaacacgc ccaaaggccg cgtcatcgcc aatatgattg
     4321 tcgtcaaccg aggcggcgat ttgctgctga ttatggcgca agacctgctt gaagcaacgg
     4381 tcaaacgttt gcggatgttt gtgttacgcg ccaaagccgt ttttgaaatc cttgaagatt
     4441 acgccgtcgg cgcggaatta gaagcatccg ccgaaccgct tgccgcgcaa gaacccagcc
     4501 tcgcctttac cgccgaatgc ggttcagacg gcatctgttc cgtcgtcctg ccacatcggg
     4561 gcattttgca catcgccccc aaaaacgcct tgccgcctta tgatgccgcc gccgaaaacg
     4621 cgtggcggct gcacgaaatc cgtagcggtt atccgtggat atgcgcggct accaaagaaa
     4681 ccgccgtcgc ccaaatgctc aaccagcaca tcatcggcgg cgtacacttc aaaaaaggct
     4741 gctatccggg acaggaaatc attgcccgtg cccagtatcg ggggcaggtc aaacgcggct
     4801 tggcggtatt gtcgggaaat tcggccgtcg aagcaggaac cctgctgacg gcggacggcg
     4861 aagaggccgg catcgtactc gacagcgttc aagattcgga aaactttacc gcccttaccg
     4921 tgattaaatt ctccgccgca caaaaaacac tgaccgtccc aaacggcggt atcttcaaag
     4981 cggttcatct cttctttaaa accgaaaatg cagaataagc cgccgatgcc gtctgaagcc
     5041 cttcagacgg cattgccgcc ccgccaatcg gttacaatgc cgcctttcta attttcaaac
     5101 ccaaaccgcc atgtccaaaa aaaccaaaca agaactcgaa aacaacaaac tcagcaaacg
     5161 cctgcgccac gccgtcggcg acgcgattaa cgatttcaat atgatcgagc cggacgacaa
     5221 aatcatggtc tgcctctccg gcggcaaaga cagctatgcc ctgttagaca tcctgcgcca
     5281 gctccaagcc agcgcgccga ttgatttcca actggttgcc gtcaatctcg accaaaagca
     5341 gccgggcttc cccgaagaag tattgccgac ctatctcgaa agcatcggcg ttccctacaa
     5401 aatcgttgaa gaagacacct actccaccgt caaacgcgta ttagacgaag gcaaaacgac
     5461 ttgttcgctg tgcagccgcc tgcgccgcgg catcctctac cgcactgcaa aagaattggg
     5521 ctgcaccaaa atcgccttgg gacaccaccg cgacgacatc ctcgccacct tgtttttaaa
     5581 tatgttttac ggcggcaaac tcaaagccat gccgcccaag ctggtgagcg acaacggcga
     5641 acacatcgtc atccgcccgc tggcgtatgt gaaagaaaaa gacttaatca aatacgccga
     5701 attaaaacaa ttccccatca tcccgtgcaa cctctgcggc tcgcaaccca acctgcaacg
     5761 ccaagtcatc ggcgatatgc tgcgcgattg ggacaaacgc ttccccggcc gcatcgaatc
     5821 gatgttctcc gccctgcaaa acgtcgtccc ttcccacctt gccgataccg aactcttcga
     5881 ctttgtcgga ttggaacgcg gtcaaagcct gaaacacggc ggcgatttgg cgtttgacag
     5941 cgaaaaaatg cccgaacgtt tttcagacgg cagcgaagaa gacgagagcg aaatcaaaat
     6001 cgagccgcaa aaagccgaac gcaaagtcat caatattctg gcgaacaagc cgaaaacctg
     6061 cggctcatag acctccgccg tcaaaaatgc cgtctgaagc ccgtttggcg tttcagacg
//
LOCUS       DAWIVB010000129         6048 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWIVB010000129 DAWIVB010000000
VERSION     DAWIVB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.129"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            20..403
                     /locus_tag="WIX60_001601"
     CDS             20..403
                     /locus_tag="WIX60_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ5305848.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            696..1169
                     /locus_tag="WIX60_001602"
     CDS             696..1169
                     /locus_tag="WIX60_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ5305849.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            1295..2398
                     /gene="mnmA"
                     /locus_tag="WIX60_001603"
     CDS             1295..2398
                     /gene="mnmA"
                     /locus_tag="WIX60_001603"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ5305850.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYEGDVCLGGGIIQSTDKPVIITR"
     gene            2469..4139
                     /locus_tag="WIX60_001604"
     CDS             2469..4139
                     /locus_tag="WIX60_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ5305851.1"
                     /translation="MKKIWLDSYEKGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVRGPQVMKGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4251..5885
                     /locus_tag="WIX60_001605"
     CDS             4251..5885
                     /locus_tag="WIX60_001605"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ5305852.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRSNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDREMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHNQGIDSIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1582 a         1619 c         1536 g         1311 t
ORIGIN      
        1 acattttgaa aggataccga tggaaccttc ttcttacgcg gcagaaaaaa aaggcaaagg
       61 cggcatcagg cgcgtcatta acgcattcgg ctattcgata gacggcatcg ccgccgccta
      121 ccgttacgaa gcggcattcc gtcaggtttt gtggctgaac gtactgctgg tgtgcgcggc
      181 atttttttgg gtttccgaaa ccgccgtccg cctgccgttg attatcgcgt cttttgtgtc
      241 ggtcattgtc gaactgttca acaccgccgt cgaagctgcc gtcgatcata cttcgaccga
      301 aaaacacgag ctggccaaac gcgccaaaga cgcaggttct gccgcacaat tgttcgcgat
      361 gctgatgttg gcggcggttt ggctgtctgc cctgttcggg taaaacgctt gcagcaggat
      421 ttgtaatcct ttaggattag tatgtggtat ttattattca cttaatctac atcaaatttc
      481 caggcagtat ttgtatgtat agtggattaa caaaaatcag gacaaggcga cgaagccgca
      541 gacagtacaa atagtacgga accgatccac ttggtgcttc agcaccttag agaatcgttc
      601 tctttgagct aaggcgaggc aacgctgtac tggtttttgt taatccgcta taagattaag
      661 cacattcccc gtctgatatt ataaggagca gaaagatgaa aaaattattg gcggccgtga
      721 tgatggcagg tttggcaggc gcggtttccg ccgccggagt ccacgttgag gacggctggg
      781 cgcgcaccac cgtcgaaggt atgaaaatag gcggcgcgtt catgaaaatc cacaacgacg
      841 aagccaaaca agactttttg ctcggcggaa gcagccccgt tgccgaccgc gtcgaagtgc
      901 atacccacat caacgacaac ggcgtgatgc ggatgcgcga agtcgaaggc ggcgtgcctt
      961 tggaagcgaa atccgttacc gaactcaaac ccggcagcta tcatgtgatg tttatgggtt
     1021 tgaaaaaaca attaaaagag ggcgataaaa ttcccgttac cctgaaattt aaaaacgcca
     1081 aagcgcaaac cgtccaactg gaagtcaaaa ccgcgccgat gccggcaatg aaccacggtc
     1141 atcaccacgg cgaagcgcat cagcactaat ctgctgaaaa tatttgaaat gccgtctgaa
     1201 aaagcccggg ctttcagacg gcatttttat gactgccttt aaaatgtgtt aaaatccgcc
     1261 tttaaaaacc gccgtttcca accatattct acgcatgaat acgacagcaa acccttccaa
     1321 tatcatcgtc gggctctccg gcggtgtcga ttcttccgta accgccgccc tgctcaagca
     1381 gcagggttat caagtgcgcg gtgtgttcat gcagaactgg gaagacgacg acaacgacga
     1441 atattgcagc atcaaacagg attcgttcga tgccatcgcc gttgccgata ttatcggcat
     1501 cgatatcgac atcgttaatt tcgccgcgca atataaagac aaagtctttg cttattttct
     1561 tcaggaatac agtgcggggc gcacgccgaa tcccgatgtg ttgtgcaacg ccgaaatcaa
     1621 attcaaatgc tttttggatt acgccgtagg gcagggcgcg gataccatcg ccaccggaca
     1681 ctatgcgcgc aaagaagtcc gcaacggcgt gcattacctg ctcaaaggtt tggatcgaaa
     1741 caaagaccaa agctatttcc tctaccgcct caagcctttc caactcgagc gcgcgatttt
     1801 tccgttgggc ggtttggaaa aacccgaagt ccgccgcctt gccgccgagt tcaaactgcc
     1861 aaccgctgcc aaaaaagaca gcacgggcat ctgcttcatc ggcgaacgtc cgtttcgcga
     1921 gttcctgcag aaatacctgc cgaccgacaa cggtaaaatg gttacgcccg aagggaaaac
     1981 cgtcggcgaa cacgtcgggc tgatgttcta cacgctcgga cagcgcaaag gtttgggcat
     2041 tggcggtgcg ggcgaaccgt ggtttgttgc ggctaaagat ttgacgaaaa acgaactgat
     2101 tgtcgtacaa ggacacgacc atccgctgct ctatacccgc agccttgtga tgaacgattt
     2161 gagtttcacg ctgcccgaac gcccgaaagc agggcgttat acctgtaaaa cgcgttaccg
     2221 tatggcggac gcgccttgtg agttgcgcta tttggatgaa gaaaccgccg agctggtgtt
     2281 tgacgaaccg caatgggcgg taacgcccgg tcaatccgcc gtgctgtatg agggcgatgt
     2341 ctgcttgggc ggcggcatta tccaatcaac cgacaaaccc gtcatcatca cgcgataaag
     2401 gtaatgccgt ctgaaacggt tttcagacgg cattgttccg ctcaattcca ctttaaagac
     2461 cgatacctat gaaaaaaatc tggttagaca gctacgagaa gggcgtgaat gccgaaatcg
     2521 acatcacgca atacggttcc gtcagcgatg tattttgcca aagtgtcaag aaatttgccg
     2581 gccaacctgc ttttcaaaac atgggcaaga cgctgactta tgccgaaacc ggcaaactgg
     2641 caacagattt tgcttcttat ctgcaaaacg tcctgaagct gccgcgcggc gaacgtgttg
     2701 ccattatgct gcccaacgta ttgcaatacc cgattgccct tttcggcatt ttgcaggcag
     2761 gtttggtagc ggtgaacacc aatccgctct ataccccgcg cgagctggag catcagttga
     2821 aagacagcgg cgcgaccgcc atcatcgttt tggaaaattt cgccaacacg ctggagctgg
     2881 tgctgccgcg cacgcagatc aaacacgtca tcgtagcctc cgtcggcgaa atgttcgggc
     2941 tgcttaaagg ttcgctgatc aatttcgtca tccggaaaat caagaaaatg gtgcccgaat
     3001 accgtattcg ggaaaccatt tcctttcaga cggcattgaa agagggggcg aagcacgttt
     3061 tccaacctgt cgcattaaac cgcgaagata ccgcgctgtt gcaatacacg ggcggcacga
     3121 caggtgttgc caaaggggcg gtgctgagcc acggcaacat ctgcgccaat atgcttcagg
     3181 caaaagaatg gattaaaaac cagttgcgtg agggaaaaga aaccgttatc gccgccttgc
     3241 cgctgtacca tatttttgcc ttaaccgtga atctgatgat ttttgccaat gccggctcga
     3301 aaattgtcct gattaccaac ccgcgcgata tgaaaggctt tatcggagaa ctgaaaaaac
     3361 agcgggttaa tgtatttatc ggcgtgaaca cgctgtttaa cgcgatggtt aaccgtcctg
     3421 attttgccga agtcgatttt tcaggattgc ggctgacttt gggcggcggt atggcgaccc
     3481 aaaaagccgt tgccgaaaaa tggaaaaaaa tcaccgacac gcccatcgtc gaagcctacg
     3541 gtctgaccga agccagcccc ggcgtgtgct gcaacccctt aaacatcgaa tcatacagcg
     3601 gcagcatcgg tttgcccgtc tcgtccaccg aagtcgaact gcgcgacgca aacggcaaag
     3661 aagtccccgt cgggcagccg ggcgaattgt gggtgcgcgg cccgcaagtc atgaaaggct
     3721 attggaaccg tcccgaagaa accgctaaag ccatagacgc gcgcggcttc ttggaaaccg
     3781 gcgatattgc cgtcatggac gaaaaaggct ggttgaagct ggtcgatcgt aagaaagacc
     3841 tcgtcgttgt ttccggattc aatgtttatc cgaacgaaat cgaggaagtc atcgcgcatc
     3901 acggcaaagt catggaagtc gcctgtatcg gcgttcccga cgaaaaaacc ggcgaggcac
     3961 tcaaagtatt cgtcgtcaaa aaagacccgt cattgaccaa agaagaactt atcgcctttt
     4021 gccgcaccga attaaccgca tataaagtac cgaaaaatat cgaattccgc gacgagttgc
     4081 ccaagtccaa cgtcggcaaa atcctgcgcc gcgagttgcg gcaaagtacc gggaaataaa
     4141 gaaaagatgc cgtctgaaaa cagccgtcca ccgttcagac ggcatccgtc cgtttgccag
     4201 aaccgcgcgc ttcacgttaa aatcacgcat tccaacatgg gtattccatc atgaccaaat
     4261 tcattttcgt aaccggcggc gttgtctcct cactgggtaa aggtatcgcc gccgcttcta
     4321 ttgccgccat cctcgaatcg cgcggcttga acgttaccat gctcaagctc gatccttata
     4381 tcaacgtcga tcccggcacg atgagcccgt tccaacacgg cgaagtgttc gtaaccgacg
     4441 acggcgcgga aaccgacctc gacttgggac actacgaacg tttcatcgat tccacgatga
     4501 cccgccgcaa cagcttcagc acggggcagg tgtacgaaaa cgtcatcgcc aaagaacgac
     4561 ggggcgacta cctcggcggc acggttcaag tcattccgca cattaccgac gaaatcaaac
     4621 gccgcatcca cgaaggcgcg gcaggttatg atgtggcgat tgtcgaaatc ggcggcacgg
     4681 tcggcgacat cgaatcgctg ccgtttttgg aagccatccg ccaaatgcga agccagttgg
     4741 gacgcagcaa caccctgttc gcccacttga gctacgtccc ctacatcgcc gccgccggcg
     4801 aaatcaaaac caaaccgacc cagcatactg ttaaagaaat gttgagcatc ggtttgcaac
     4861 cggacatcct gatctgccgt atggacagag aaatgcctgc ggacgagcgc cgtaagattg
     4921 cattgttctg taacgttgaa gaacgtgcca tcgtgggcag ttacgacgtg gacagcattt
     4981 acgaatgccc tgaaatgctg cacaaccaag gcatcgacag cattattacc gagcagttgc
     5041 agcttaatgt gcagcaggcg gatttgaccg cgtggaaaaa aatcgtccac gccatccaaa
     5101 acccgaaaca caccgtcaaa atcgccatgg tcggcaaata cgtcgatttg accgagtcct
     5161 acaaatcgct gatcgaagcc ttgaaacacg caggcattca caccgaaacc gacgtacaaa
     5221 ttacctttgt tgacagcgaa agcatcgaga aaaacaacgg cgacgtttct atgcttaaag
     5281 atatggacgc catcctcgtt cccggcggtt ttggttcgcg cggcgtggaa ggcaaaatcg
     5341 ccgctgtgcg ctatgcccgc gaaaacaacg tgccatactt gggcatctgc ctcggtatgc
     5401 agattgcgct gatcgaatac gcccgcgacg tggcaggttt gaaaggtgcg aattccaccg
     5461 agttcgacct caaatgcgcc gcccccgtcg ttgccctgat tgacgaatgg caaactgccg
     5521 acggcagcgt cgaaacccgc gacgaatccg ccgatttggg cggcaccatg cgtttgggcg
     5581 cgcaagaggt tgagttgaaa gcaggcagcc tcgccgccaa aatctacggc agcgaacaca
     5641 tccgcgaacg ccaccgccac cgctacgaag tcaacaacaa ctacgttcct acgctggaac
     5701 aggcaggttt ggtcatcggc ggcgtatccg ccggacgcga acgcttggtc gaaaccatcg
     5761 aactgccgaa ccatccttgg ttcttcgcct gccagttcca ccccgagttt acctccaatc
     5821 cgcgcaaagg gcatcctttg ttcaccgcgt ttgtcaaagc tgcgttgaac aataaaaaag
     5881 cctgataaag cgttacttga tgatcaaaat gccgtctgaa agcctgaatg aagactttca
     5941 gacggcattt tcgcaaatcg gggattataa aagccatcaa tttaattttt atggttttat
     6001 ctaatattga ccaactgaat attgttaagc ggggcgtgaa acttaagt
//
LOCUS       DAWIVB010000130         5985 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWIVB010000130 DAWIVB010000000
VERSION     DAWIVB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.130"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(20..1423)
                     /gene="mtrE"
                     /locus_tag="WIX60_001606"
     CDS             complement(20..1423)
                     /gene="mtrE"
                     /locus_tag="WIX60_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ5305853.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQVQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1478..4681)
                     /gene="mtrD"
                     /locus_tag="WIX60_001607"
     CDS             complement(1478..4681)
                     /gene="mtrD"
                     /locus_tag="WIX60_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ5305854.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4693..5931)
                     /gene="mtrC"
                     /locus_tag="WIX60_001608"
     CDS             complement(4693..5931)
                     /gene="mtrC"
                     /locus_tag="WIX60_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ5305855.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGISLNRSRITAPISGFIGQSKVSEGTLLNAGDATVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWASSENQA
                     AAPQSGVQTASEAKPASEAK"
BASE COUNT         1336 a         1707 c         1617 g         1325 t
ORIGIN      
        1 cattttattg cccgaccggt tatttgtcgg tttgggtatc ccgtttcaat ccgccgccga
       61 gtgccttgta caaatcggca aggttttcgg cgcgggtcag ttgtgccgac aaagccgcac
      121 cctccgccgc atagctgctg cgttccgcat cgagcaagtc gagcgcgccg gatacgccgt
      181 gcttgtaacg caggccgacc aagcgcaacg cttctttaga ggcgcggctt tgtttgctta
      241 aagcgtcata ggctttatcc agctgctcgc gcgccgccaa tgcgtttgcc acgtcttgaa
      301 atgcggattg gacggcggat tcataggcaa cgatttgtac ctgttggcgc agcttggcta
      361 catcaaggtt cgccttgttc gtaccccagg taaaaatcgg cagggtaata gacggcgcga
      421 acgaccaaac gcccgtgccg cttttgaaca acccacccaa ttcggcagaa cccgtaccga
      481 cggttccggt caggcggatg gatgggaaaa aggcggcgcg tgccgcaccg atattggcgt
      541 ttgcctgttt gagcgcgtgt tcggcagcac ggatatcggg acggtcgagc aatacttcgg
      601 aactcaaacc ggccggcagt ttttcaacaa aaaactgctt gtccagcggc aaaccggcag
      661 gcaggtcttc gggtatcggt tggttaatca aggttgccaa ggcattgcgc gcctgttcgc
      721 ggctgcgcgc ggcatgggca taatcggctt tggcagattc gatcagggct tcctgctgac
      781 gtagggcgac ggcggaaatc acgcctgcct tgtaacgtaa ttcggacagc ttgtaggttt
      841 cctcgcgcgt tttcaaaaca cgttgcgcca aagacatcgc ttcttcggcg taacgttcgt
      901 tgaaataggc tttggcaacg gtggcaatca ggctcaaatg tgccgcatcg cggttggcgg
      961 tgctggcgaa atagccttgc agtgccgcct cgctgctgct gcgtacacgc ccgaacagat
     1021 cgagttcgta agatgccgca cccagtccga ctttgtagct gctgcttaca ttgccgccgc
     1081 tcaagctgcc ttggcgcgag tcgttcgcat tggcggcaag cgtgggcagg aggttgttgc
     1141 gctcaatcat gtattgtttg cggtagattt cgctgttcaa tacggcggta cgcaaactgg
     1201 tattgcgctc gagtgcgatg tcgatcagct tttgcaggcg cgggtcggca aaatagtcat
     1261 gccaacctaa atcgacggcg cggatgccgc tgtcggcggt atcgtttttg aacgtttcgg
     1321 caacttcgac tttgggctgc tcgtattggg gaatcatggt gcaggcagac aatgcaaagg
     1381 ctgctgcaac agaagtcaag gtggttttca atgtagtatc cataaaaaag tcctgatgcc
     1441 gtctgaaaac ccgtgggcgt tcagacggca tggttgctta atgttggctg tcgtccgaac
     1501 cggtgatgcc cgcttcggcg gcgtgtttta ctgccatttc gtgttcgtgc gcggtttctt
     1561 tgaagaattt gcgcaccacc acatagaaaa gcggaacaag gaacacggac aagagcgtgc
     1621 cgatgagcat cccccagaat acggttgtac cgatggcgcg ctggctggca gaacttgcac
     1681 cgccggcaat atacagggga accacgccca aaataaaggc gaacgaggtc atgataatcg
     1741 gacggaaacg caggcgggcg gcttccaaag cggcttcaac cgcgcttttc ccttgcgctt
     1801 gaaggtcttt ggcaaattcg ataatcaaaa tcgcattttt cgcactcaaa cccatcacgg
     1861 taacgaaacc gacttgaaag tagatgtcgt tggcgaacga gggaacgctg cccaacagtc
     1921 cttcaaacag gttgcgcccg gttacgcccg cagccgcacc gatcaaaccc aacggaatca
     1981 caaggatgac cgccagcgga atcgaccagc tttcataaag cgcggcaagt accaaaaata
     2041 cggctgcaac cgccaaaccg tacaaaatca gggtttgcga gccgcctttt gcctcttcgc
     2101 gcgactgtcc gccccactcc aggctgtaac cgccgcccaa ttcgtcaacc attttttgaa
     2161 ccgccgccat agcctgcccg gtggaaacgc cggttgcagg cgaagcggac agcttcatcg
     2221 aaggataacc gttgaagcgt acgctctgtt ccgtaccgtt ttcccaagaa acagtagcaa
     2281 tggtggaaag cggtacggcg acgccggatt tgttcggcac ggtcaggttc aaaatatcgg
     2341 caggctgcat acgggcatcc tcgtcggcct gcaccatcac gcgttgcaga cggccttggt
     2401 tcgggaagtc gctgacataa gacgaactca gcgcgcttgc caatgcggtg cggatgtcgg
     2461 caaacgaaat gccttgcgcc gccgccgcgg cacggttgat gtcgattttc aactgcggcg
     2521 agtcttccaa accgccagca cggacggtgc tggggtcaaa caaaccgctg gcacgcattt
     2581 tctgaatcaa ctcgttgcgc ttcgccagca atgcggtatg gccggtattg ttgcggtctt
     2641 gcaggttgat gctcagaccc gaaccgttgc ccaactccag aatcggaggc gggacgacgg
     2701 cgatgccaaa accgtcttta agcgtcccca tcatcatacc cgtcagcttg ccggcaatcg
     2761 caacggcatc gctgccgggc gcggtacgct cgttccaatc tttcaatatg gcaaaaccca
     2821 tcgccatatt ctgaccgctg cccgaaaagc tgaagccgga aacggtaatg atgttttcta
     2881 tttcaggaat gcttttcgcc agttgggtaa cttgcgccaa agtcgcattg gtgcgctctt
     2941 gggtcgctcc tgcaggcagt tgcacgctga ccatgacgaa gccttggtct tcggtcggca
     3001 ggaatgaagt cggcaggcgc ataaacagga acacgcccac aaccgccaag ccgatataga
     3061 caaccatcat gcggaaagtc ttacgcagca ctttggcaac ccggccttcg taaccgtgcg
     3121 tccaactgtt gaatttcttg ttaaaccagc cgaagaaacc ttttttctct tcgtgatgcc
     3181 ctttcgggat tgtcttcaac attgtggcac acaaagcagg ggtaagggtc agcgcaagga
     3241 aggcggagaa tgcgattgat gacgccatcg tcagggcaaa ctgtttgtaa atattgcccg
     3301 tcgccccgct gaacatcgcc aacggtacga acacggaaat cagaacggcg gtaataccga
     3361 tgaccgcgcc cgaaatctga cccatcgctt ttttggtcgc ttctttgggc ggcaagcctt
     3421 cacccgccat aatgcgctcg acgttttcaa ccaccacaat cgcgtcatcg accacgatgc
     3481 cgatgaccaa aaccatcgca aacatggtca gtacgttaat cgacatgccc atataagaga
     3541 tgaaggcgaa accgcccaac agcgaaatcg gtacgacgat ggtcggaatc agcgtataac
     3601 ggatgttttg caggaagaga tacattacga caaacaccag caccatcgct tcgattaaag
     3661 tgtgaatcac tttttcaatc gaaatttcga cgaatttgga agtatcgtaa ggggttttcc
     3721 agctcatacc ctgaggaaag tatttttcca acgtcgccat gcgttcttta accgcctttg
     3781 ccgtcgccat cgcattgccg ctgttggaca gcatcaccgc cataccggtg gtatttacac
     3841 cgttcagacg ggttgaggaa gaatagtctt ccatacccag tccgaccctt gccacatcct
     3901 tcaggtaaac attagaaccg tcggtattgg cgcggaggat gacgttgccg aattcttctg
     3961 ccgtacccaa ctgcccttgc gccgttacgg tagccgtaac cgtctgtccg cgaacggcgg
     4021 gaagcgaacc gatagaaccc gctgaaatct ggacgttctg ggcggacagc gcgctgccaa
     4081 catcggcaaa cgacaaattg tagttttgca gtttcttagg atcaacccaa atccgcatcg
     4141 cgcgttgcgc gccgaacagg cgtacctgcc ccacgccttc gatacgctgc aactcgggaa
     4201 cgatattacg ctgcgcgtag tcgttcatct cttcggttga ctgcacatcc gacgaaagca
     4261 tcacaatcat caggaaattg gaacgcgcct tggatacggt tacgccgtat tgctggacag
     4321 ttgccggcag cgtgctcaat acttcggaaa gcttgttctg cacttccacc tgcgccagat
     4381 tctcgtcggt atcgggcgta aaggtcaggc tcacactgcc gctgccgctc gaatcggcgg
     4441 aagtggacat ataatccaaa ccttccacgc cgttcatatt ccgctcgatc acggaaagca
     4501 cgctgtcttc cattacctgc gcggacgcgc ccggataagt ggccctcagg gtgatggtcg
     4561 gggcggcgac ggacggatat tgcgaaaccg gcaggctttt gatgccgaaa atacccgccg
     4621 caataatgaa aatcgaaata acccacgcaa aaatggggcg gtcgataaaa aatttagcca
     4681 tcgatgcctt ccttatttcg cttcagaagc aggtttggct tcagatgccg tctgaacgcc
     4741 ggattgaggc gcggcggctt ggttttcaga cgacgcccat tctttgggcg ttaccttttt
     4801 cgcacccgtt ataccggcga tactgatgcc ttccacaacc accttgtccc cgtccttcag
     4861 acccgacgta acaatccaat tcgtaccctg ctgttgcgca accgttacct cgcggggttc
     4921 cataccgcct tgggcattca caatcatcac ggtatctttc gcaccgcgcg ttaccgcctg
     4981 ctgcggcaca acaaatgcgt tatccaccgc cacttggtcc atcagcacgc gcacatacag
     5041 accgggcatc aagatattct gatcgttcgg tacggcggcg cgcagggtaa tctgaccggt
     5101 cgattcgttg acggccggat cggcaaacag caggcggcct ttttcagggt aaactgtgcc
     5161 gtcgtcaaat ttgatgccga ccgcaatcac accatccgcc gccagcagtt tgccttcggc
     5221 tatctgacgg cgcaatttca tcacttcgga tgcagactgg gtaacgttca catacatcgg
     5281 attggtttgg cggatggtcg ccagtacggt cgcatcgcca gcgttcagca acgtaccttc
     5341 ggaaactttg gactgaccga taaagccgga aatcggcgcg gtaatgcgcg aacggttcag
     5401 gctgatgccg gcggatttga ttgccgcctg cgccgcttta acgcctgcct cggcagaacg
     5461 tttcgccgtt accgcagcat cgtattcctg ccggctgacg gcttcggcgg caaccaaagg
     5521 cttgtatcgc gccaaatccg catccgcttt ggcaagcgtt gcctgagccg ttgccagttg
     5581 cgcgcgcgcg ctttccagac ctgcttcata agtggaactg tcgatctgat acagcggctg
     5641 tccggcacgg acataactgc cttcttggaa caggcgtttt tggatgatgc cgccgacttg
     5701 ggcgcggaca tcggcggtac gcagcgattc caaacgcccc ggcaactcga cggtcaatgc
     5761 gacggtttgc ggatggacgg ttacgacacc gacgacgggc gcaggggctt cccgaccagc
     5821 aggctgcccg ccctgcgccg cgtctccgcc tttaccgcaa gacgacagta ccaatgcaac
     5881 ggcggcagcc aacgcggccg cacgcatcgc cttaaaagca taaaaagcca ttatttatcc
     5941 tatctgtctg gtttgatgta aagggttttg ccaatcaaca ggcat
//
LOCUS       DAWIVB010000131         5904 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWIVB010000131 DAWIVB010000000
VERSION     DAWIVB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.131"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(110..469)
                     /gene="rplT"
                     /locus_tag="WIX60_001609"
     CDS             complement(110..469)
                     /gene="rplT"
                     /locus_tag="WIX60_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ5305856.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGTRENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WIX60_001610"
     CDS             complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WIX60_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ5305857.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(826..1347)
                     /gene="infC"
                     /locus_tag="WIX60_001611"
     CDS             complement(826..1347)
                     /gene="infC"
                     /locus_tag="WIX60_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ5305858.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WIX60_001612"
     CDS             complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WIX60_001612"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ5305859.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKATVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLIRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3331..3407)
                     /locus_tag="WIX60_001613"
     tRNA            complement(3331..3407)
                     /locus_tag="WIX60_001613"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3371..3373),aa:Val,seq:gac)
     gene            3586..4701
                     /gene="tgt"
                     /locus_tag="WIX60_001614"
     CDS             3586..4701
                     /gene="tgt"
                     /locus_tag="WIX60_001614"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ5305860.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            4711..5793
                     /locus_tag="WIX60_001615"
     CDS             4711..5793
                     /locus_tag="WIX60_001615"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ5305861.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGSWR"
BASE COUNT         1465 a         1558 c         1397 g         1484 t
ORIGIN      
        1 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
       61 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
      121 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
      181 cagccaatac tttgcggtcg atttcaatag aggcgcgttt cagaccgttc ataaatttgc
      241 tgtaagacaa cccgttttca cgcgtacctg cattgatacg gacaatccac aattgacgga
      301 attggcgttt gcgttggcgg cggtcacggt acgcgtattg accggctttc attaccgcct
      361 gcttggcaac gcggtaaacg tttttacgac ggccgcggta gcctttggct aacgcgaaga
      421 ttttttggtg acgggcacga gcggtaacac cgcgttttac gcgtggcata ttctaaactc
      481 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcattt accatagagg
      541 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
      601 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
      661 tggttttcat tttaggcatg ggaaaactcc attcgttatc ggataaggca ttagggggtt
      721 ttaaaacatc ggtttcaaac acttgaaccc acaatgccac ggttttttgc cgcaattgaa
      781 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
      841 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
      901 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
      961 ttcacggccg cggaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     1021 aatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttgatttc
     1081 cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgtttcttgg cctgctggta
     1141 tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg gggaaatctc
     1201 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     1261 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     1321 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     1381 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     1441 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     1501 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     1561 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcccgacaa
     1621 taatctgcct gattttcggt aatattcata attaccaatt gaaccggagc caaccataac
     1681 gggaatgagc ctgcatggtt ctcaatcaga atgccgataa accgctccaa agaacctaaa
     1741 atggcgcgat gcaacataac aggacgcgca cggtcgttgt tttcagttac atattcggca
     1801 ttcaaacgct ccggcaatac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     1861 aaggcatctc tgacatgata ttcgatttta ggcccgtaaa acgcaccctc gcccggcaat
     1921 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     1981 cacacgtcat ctgaacctgc gcgtttttca gggcgaagag aaagcttgac ggatacatca
     2041 tggaaaccga actgtttgta gatgcgaatc aacaattcat tgaacgcacg agcctcgctg
     2101 acgatttgat cttcggtaca gaaaatatgc gcatcatcct gcacaaaacc gcgaacccgc
     2161 atcagaccgt gcagcgcacc gctcggctca ttgcggtggc aagaaccgaa ttccgccaaa
     2221 cgcatcggca aatctcgata cgaacgcaaa ccgttgttaa aaatttgaac atgacccgga
     2281 cagttcatcg gtttaaccgc atattcgcgt ttttccgaac tggttacgaa catattatct
     2341 ttgtagttgt cccaatggcc ggatttttcc caaaaggttt tatccatgat ttgaggcgtt
     2401 ttgacctctt tataaccggc ggcgttcagc tctttacgca tatgctgttc aatcacttgc
     2461 cacaaagccc agcctttagg atgccaaaac accatgcccg gcgcttcgtc ttgcaggtgg
     2521 aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     2581 tgaatatagg cttttaattc gtcttttgtc gcccaagccg taccgtatat gcgttgcagc
     2641 atttcattat tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     2701 ttcaggaaac gggtattcgg aacgtgcggg ccgcggcaca tatcgacata ttcctggtga
     2761 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     2821 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     2881 tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     2941 gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct cttcaatgac ggggccgata
     3001 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg cgcgcaggaa
     3061 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaatct
     3121 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc caccgtcgcc
     3181 ttcgccaaac cggcaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     3241 tggcggactg agccgtccgg caaggtaata ttcaacatca aacgctccga aagattaaaa
     3301 aataacggca taaatgccgt tatgggaatt tggtaggcac gattggattc gaaccaacga
     3361 cccccaccat gtcaaggtgg tgctctaacc aactgagcta cgtgccttca aagaattttt
     3421 gcattttatc gggtcgtttt aatttttgca agaggtatgt gcgtttcttt atgcaacatt
     3481 gcggcaagat ttggggatgt cgtctgaaaa accgtgggaa atcatttata atgccgtctt
     3541 ttaaaatata tttcgacccc tgtcgcccaa ctgacattca atcttatgct taaatttacc
     3601 ttacacaaaa aagacggtct cgcccgacgc ggcacgctgg agttgaacca cggcaaaatt
     3661 gaaacgccgg tatttatgcc tgtcggtact tacggttcgg ttaaggcgat gaacccgcaa
     3721 aacctgcacg atatcaaggc acaaattatt ttgggcaaca cttatcattt gtggctgcgt
     3781 ccgggtttgg aagttatcgg gcaattcggc ggcctgcacg gttttatcgg ctgggacaaa
     3841 ccgattctga ccgactcggg cggttttcag gttttttctt tgtcggatat gcgcaagctg
     3901 acggaagaag gctgtacgtt caaaagcccg attaacggcg acaagctgtt tttatcgcct
     3961 gaaatttcca tgaaaatcca aacggttttg aactcggata tcgcgatgca gttggacgaa
     4021 tgcacgccgg gcgaggcgac gcgcgaacag gcgcgaaaat cgctgcaaat gagcctgcgc
     4081 tgggcggaac ggagcaaaaa agcctttgaa gatttgaaaa acccgaacgc gctgttcggc
     4141 atcgtgcaag gcgcgatgta tgaggatttg cgcgaagaat cgctgcgcgg tttggaacag
     4201 tttgattttc ccggccttgc cgtcggcggt ttgtccgtcg gcgaacccaa gcccgaaatg
     4261 taccgtatgc tgcgcgccgt cggtccgatt ctgccggaac acaagccgca ttacctgatg
     4321 ggcgtaggca cgccggaaga cctcgtgtac ggcgtggcac acggcatcga tatgttcgac
     4381 tgcgtgatgc ccacccgcaa tgcacgcaac ggctggctgt ttacccgttt cggcgatttg
     4441 aaaatcaaaa acgccaaaca caagctcgac aagcgtccga tagacgaaag ctgcacctgc
     4501 tacgcctgcc aaaacttcag ccgcgcctac ctgcaccatc tgcaccgcac cggcgaaatc
     4561 ttgggcgcac agttgaacac catccacaat ctgcatttct accaagtcat tatggcggaa
     4621 atgcgcgaag ccgtcgaaca aggcaaattt gccgactggc aggcgcgatt ccacgaaaac
     4681 cgcgcacgcg gcacagactg attccctgca atgccgtctg aagccgcttc agacggcatt
     4741 ttgccgcttt accctacccg cactattttt cagaaaccga tcatgcttcc attctttcca
     4801 gaaccctccc tttcctatac gcaacaaaac cgtaccgccg ttttgctgtt gaacctcggc
     4861 acgcccgacg cgccgaccgc gcaggcggta cgaccttatc tgaagtcctt tctgaccgac
     4921 cggcgcgtcg tcgaactgcc caaatggctg tggtatccca ttctgcatgg tctggtactg
     4981 acgctccgcc ccaaaaaaag cgcgcacgcc tatgaaaaaa tctggtttaa ggaaggttcg
     5041 cccttagaag tttacacagc acgccaagct gccgcgcttg ccaaacgtat gcccgacctc
     5101 atcgtccgcc acgccatgac ctacggcaat ccttcggtgg cagacgtatt gtcggaactg
     5161 aaggcgcaag gcgcgggcag actgctggtc atcccgatgt acccgcaata tgccgcttcc
     5221 agcagcggag cggcagtaga taaggtgtgc gaacaattac tgctgcaacg gaaccaaatg
     5281 agcgtccgca ccgtttcgcg cttttatgac gataccggct acattgatgc gatgaaaaac
     5341 cacatcctcc gatattgggc ggaacacggg cgcgggaaaa aactgatgtt gagttttcac
     5401 ggcgtaccgc agaagcacca cgacctcggc gacccctatc ccgacgagtg ccgccacacc
     5461 gccaaactgc ttgccgaagc actggaactg accgaagacc aatatgtcgt atcgttccaa
     5521 agccaattcg gcagggcaaa atgggttaca ccgagcacgc aggatttgtt cggcaaactg
     5581 cccaaacagg gggtaaccga gctggacgta ttttgcccgg gctttttggc agactgtttg
     5641 gaaaccatgg aagaaatcgc cctgatgggg cgggaacagt tttatgaagc aggcggcaaa
     5701 agctaccgct acatcccctg cctcaacgac aaccccgact ggatagatgc actcgtcgca
     5761 cttgccgaag aaaaccttgg cagctggcgt tgaaactgtt tgacccatgt ctaaaatgcc
     5821 gtctgaacgc cgtgttcaga cggcattttc ccgctacttt agcgtatgat ggcatctatg
     5881 ctggacaaca acatcaagtg caac
//
LOCUS       DAWIVB010000132         5883 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWIVB010000132 DAWIVB010000000
VERSION     DAWIVB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.132"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            131..565
                     /locus_tag="WIX60_001616"
     CDS             131..565
                     /locus_tag="WIX60_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ5305862.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1441)
                     /locus_tag="WIX60_001617"
     CDS             complement(578..1441)
                     /locus_tag="WIX60_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ5305863.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1449..2066)
                     /locus_tag="WIX60_001618"
     CDS             complement(1449..2066)
                     /locus_tag="WIX60_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ5305864.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2229..2842)
                     /locus_tag="WIX60_001619"
                     /pseudo
     CDS             complement(2229..2842)
                     /locus_tag="WIX60_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
     gene            3048..4820
                     /locus_tag="WIX60_001620"
     CDS             3048..4820
                     /locus_tag="WIX60_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ5305865.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            4817..5473
                     /locus_tag="WIX60_001621"
     CDS             4817..5473
                     /locus_tag="WIX60_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ5305866.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
BASE COUNT         1444 a         1655 c         1364 g         1420 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gtcagctttc ccacgccgcc acggcttata tccccccgaa
      241 cgattttcaa ccgaactgcg acatacgccg actcgggctg acccaaagtc agcacaatga
      301 gctgcgtaaa atccgcaccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gccggtctgt cgtcgaaatc atttcctcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accggttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca caacgcccgt tgtttcggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggattt
      841 ccccccgcat ccccgtcgta ccggcattct cgtcaaaata ttcgggcatc aggtgccgtc
      901 tgttggaagt cgcgtaaacc aaaacgttgg cgcaacgttg agacagaccg ccgtctaacg
      961 cggttttcaa tgccttatag gtttcatcgc cgctttcaaa cgacaaatcg tcgcaaaata
     1021 cgataaattt ttccggacgt tccttcaaaa gcgtcaacag gtaaggcagg ccgattaaat
     1081 cgcttttatc gacttcgatc aggcgcaatc ccttatccgc atattcgtgt agcagggctt
     1141 tgaccagcga ggatttgcct gttccgcgcg cgccgctcat caatacattg ttcgcgggtc
     1201 tgccgacaat gaactgttcg gtattacgca ccagcaattc ggtttgcctg ccgactcccg
     1261 ccagccttac caagggaaag gtgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc
     1321 ccgcactctg ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa
     1381 gcacggcatc caaacgccgc aaaacggcat aggctttatc gagaaattcg ttcaattcca
     1441 tctctgcctc actttgcata tctttgcgcc atcagccgtt cgacggtatc gacgattgcc
     1501 tgcgtattcg gatcgatttc gatgttgatc ctgtcgccga cctttctgct gccgaacagc
     1561 gtccgttcca aagtttcggg aatcagatgg acattgaaac ggccgtcttc gactttgcct
     1621 atggtcaggc tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt
     1681 tcatgcggga gcgaaaacca aaccgtgcgg ttgaacccgt cccgttcgat ttcgacaata
     1741 ggcacggttg ccataatgtg tccgctcatg acgtgtccgc cgatttcgtc tccgaaacgc
     1801 gccgcccgtt cgatgttgac gcaatcgcct tccttcagca gccccaaatt ggtttttgcc
     1861 aaagtttccg ccattaaatc gaaactgacg cggtttcctt cgatttcggt aatcgtcagg
     1921 cagcagccgt tattggcgac cgatgcgccg cgttgcagat tgtccgccgc ctcttgcgga
     1981 agctcgacga cataagtgtg aaatgcctcc gacgggcggt ggattgccgt cagttttccc
     2041 aatccttgaa caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca
     2101 aattgtagca tctccccgcg aaaaatgccg tctgaaatgc cttcagacag cattatgcct
     2161 ccgattcggg caaaaaccgc ccggtatggc ttgacctttc ctttccacgc cggtcggcgg
     2221 tcttgccctt atccctcctg caaatcgatt tgcgtgttca agtcggcaaa atgcccgtca
     2281 aactcgaatc tgaccggccg cgccctttgc tgctgcaacc agcttcttaa tgttttcata
     2341 cccgaaaaca gatagggaat cgcgctttgc agaatctgcg ggcggatata cataatgttg
     2401 tagtgcatcg ttatcggcgt ttccacataa tacgcattgc acaacggtgt gcgtttcgac
     2461 acggtttcaa acctcgccac caaatcgtcc ggcagatacg gcatatcgca cggcacaacc
     2521 aaaagccagt cagcagccgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc
     2581 gggccgaaat gctgccactg ccgcgcatcg ggaaaaatat gcggacttct tcgagcatat
     2641 tcttccaaat tccggttggt gctgatggcg atatggctga cctgcggcct gaccctgtcg
     2701 atgacatggt ctatcagtgc cttaccccca aaagagcaag ccctttgtcc tcgcctccca
     2761 tacggctcgc ctgaccgccg gccagtatca gggcaaaagt tttcattgcg gatgttctct
     2821 tggaaaagtt cgaggttttc atgattgcag tctgccgttc ccaatgactc aaaatgccgt
     2881 ctgaagcaga cggcaaataa attcatatta tctgaatttt atcataacat gatttaacac
     2941 tgaagcggtg cggattaagt tttccttaac caattctttc tgagcagttg tatctaattc
     3001 caaagaatga tattgtttgc attatttgga acaatttttc gccgagcatg atactgccag
     3061 cccgtttttc agacggcatc agcctttccc tgcgcctgaa actcctgacc ggactgtggg
     3121 tcgggttggc ggcattgtct gtcgttttga cactgctgct ctctttgcgt ctggaaaacg
     3181 cggcctccgt catcgaagag gcgggcaact tgagaatgca ggcataccgt ctggcataca
     3241 tggcgggtga aggctcgccc cgtgcgcaaa ttgacaatca ggttgccgaa tttgaaaaaa
     3301 gtttaaaacg cattgcccaa agcgatgcca tccatccgct gattccttcg gacacccctc
     3361 ttgcttatga tttgatacaa tccatgctga ttatagattg gcaggcacac atcctccccc
     3421 cgctccagtc ctaccggcga ccgactcagg tcgatctcta ccgctttgcc ggaaacatcg
     3481 aactgttttt gcaggcattg gaaaatgcca acgaaaaaaa cacatggtgg ctcaggcgtt
     3541 ttcaatgggc aattatgttg atgacgctgg tgtcgtctgt actgatgctg ttttggcacc
     3601 agatttgggt tatccggccg ctgcaggcgt taagggaagg tgcggaacgc atcggacgga
     3661 ggtgtttcga tattccggtt cccgaaggcg gtacgccgga attcaaacag gtcgggcgtt
     3721 gtttcaatca aatgggcggc aggttgaaaa ttttatatga tgatttggaa ggacaagtcg
     3781 ccgagcagac acgcagtctc gaaaaacaaa atcaaaacct gaccctgctg taccaaacta
     3841 cacgggacct gcaccaatcc tacataccgc aacaggctgc agaacatttt ctaaaccgta
     3901 tcctgcccgc cgtaggagca gattccggca gagtttgttt ggacggcgga tccgatgttt
     3961 atgtttccat tcatcatgcg gattgcggca cagcagcttc ggatttgggg aagtaccatg
     4021 aggaaatctt ccccattgag taccagaacg aaacattggg caggctgttg ctcagctttc
     4081 caaacggcat ttctcttgat gaagacgacc gcatcctgct tcaaacacta ggcaggcaat
     4141 tgggcgtatc gcttgccggc gcaaaacagg aggaagaaaa acgcctgctt gcagtattgc
     4201 aggaacgcaa cctgattgcg caaggattac atgacagcat cgcacaagca ttaacgttcc
     4261 taaacctaca ggtacagatg ctggaaaccg cctttgccga aaacaaacgg gaggaagccg
     4321 cagaaaacat cagctttatc aaaacaggcg tgcaggaatg ttatgaagat gtccgcgaac
     4381 tgctgctcaa cttccgtacc aaaatcagca ataaagaatt tcccgaagcc gttgccgacc
     4441 tattcgcccg ctttacgcaa caaaccggga taacggtcga aaccgcctgg gaaaacggtt
     4501 cgttcctgcc gcctcaggaa gcgcagctcc aaatgatttt tatcctgcag gaaagcctgt
     4561 ccaacatccg caaacacgcc cgcgccaccc atgtaaaatt caccctttcc gaacacggcg
     4621 gacgctttac catgaccatc caagacaacg gacaaggttt cgacacggag aaaataggag
     4681 aacccacggg cagccatgtc ggactgcaca tcatgcagga gcgtgccaaa cgcatccatg
     4741 ccgttttaga aatccgttcc caagctcaac agggaaccac cgtctcattg acggttgcat
     4801 ctgaagaaag cttgaaatga ctattaaaat tattctgata gacgaccata ccctcttccg
     4861 cagcggcatt aaagcccttt tgtcgcgcca acacggtttt gaagtcatcg gcgaagccgc
     4921 agacggcctc tcgggtatca aaatgatcag tcggctgcaa cccgatgtcg tcctgcttga
     4981 ccttgatatg cccggtatga acggacgcga agcactctcc caaatcatca gcatcaatcc
     5041 gcagcaggca gtgattatgc tgaccgtttc cgaagacagc gacgatttga ccgaatgtat
     5101 gcgcatcggc gcgcgcggct acctgctgaa aaacatcaac gccgactttc tgctcgaaag
     5161 catacgcaaa gccgctgaag gcgataatgt attctcgccc gagatgaccg ccaaactcgt
     5221 caaaagcctg atttcccccc aacctgccca agggacgcag gcactctcct cacttacccc
     5281 tcgtgaactg gaaatcttgg gctatctcgc cgcaggacac agcaacaaaa tcatcgcccg
     5341 ccacctcgat cttgccgaat ccaccgtcaa agtccacgtt caaaacctgc tccgcaaact
     5401 caacctcagc agccgggtgc aggccgccgt ttacgccatc cggcacaacg tcccccaacc
     5461 tgtgccggaa taggcgttca gacggcatat taggggtttt aatccccgta cggtcattcg
     5521 gataacagac caagcatgta agtttatgcc cccataagta cgcttggcat agcagtaata
     5581 ttgttcggtt tagtgttttc cgtttgcccc tatctgatac tgcaatatca gctatgccgt
     5641 ctgaaaacgc atcatcatga tattttcaga cggcataata aaaagcggaa atactaatgc
     5701 agggtaaaat gttccattcc gaatcccata aatatacaat ggcttatatc gtttaagcat
     5761 gtgtaaccca ccctcatatc aatcaatata tagtggatta acaaaaaccg gtacggcgtt
     5821 gcctcgcctt gccgtactat ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     5881 tta
//
LOCUS       DAWIVB010000133         5848 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWIVB010000133 DAWIVB010000000
VERSION     DAWIVB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.133"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            125..847
                     /locus_tag="WIX60_001622"
                     /pseudo
     CDS             125..847
                     /locus_tag="WIX60_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
     gene            complement(1054..1536)
                     /locus_tag="WIX60_001623"
     CDS             complement(1054..1536)
                     /locus_tag="WIX60_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ5305867.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHVGDLVTFYASVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(1689..1850)
                     /locus_tag="WIX60_001624"
     CDS             complement(1689..1850)
                     /locus_tag="WIX60_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305868.1"
                     /translation="MSEQSEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(1924..2385)
                     /gene="recX"
                     /locus_tag="WIX60_001625"
     CDS             complement(1924..2385)
                     /gene="recX"
                     /locus_tag="WIX60_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ5305869.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKLAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDDGWEEDC"
     gene            complement(2453..3160)
                     /locus_tag="WIX60_001626"
     CDS             complement(2453..3160)
                     /locus_tag="WIX60_001626"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ5305870.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVVTNKNEILASELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            3562..4827
                     /locus_tag="WIX60_001627"
     CDS             3562..4827
                     /locus_tag="WIX60_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ5305871.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPSVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYAEAIAPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(4885..5691)
                     /locus_tag="WIX60_001628"
     CDS             complement(4885..5691)
                     /locus_tag="WIX60_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ5305872.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKTDFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1285 a         1380 c         1693 g         1490 t
ORIGIN      
        1 cgacaatacg ttgagcatcg gtcagattgt taaagtcaaa ccggcaggat atgccgcacc
       61 gaaaaccgca gccgtaaaaa gcaggcccgc cgtaccggct gccgcgcaac cgcccgtaca
      121 gtccgcaccc gtcgacatta acgcggcgac gcatactatt gtgcgcggcg acacggtgta
      181 caacatttcc aaacgctacc atatctctca agacgatttc cgtgcgtgga acggcatgac
      241 cgacaatatg ttgagcatcg gtcagattgt taaagtcaaa ccggcaggat atgccgcacc
      301 gaaaaccgca gccgtagaaa gcaggcccgc cgtaccggct gccgtgcaaa cccctgtgaa
      361 acccgccgcg caaccgcctg tgcagtccgc gccgcaacct gccgcgcccg ctgcggaaaa
      421 taaagcggtt cccgcgcccg ccccgcaatc tcctgccgct tcgccttccg gaacgcgttc
      481 ggtcggcggc attgtttggc agcgtccgac gcaaggtaaa gtggttgccg atttcggcgg
      541 caacaacaag ggtgtcgata ttgccggtaa tgcgggacag cccgttttgg cggcggctga
      601 cggcaaagtg gtttatgcag gttccggttt gaggggatac ggcaatctgg tcatcatcca
      661 gcataattct tccttcctga ccgcatacgg gcacaaccaa aaattgctgg tcggcgaagg
      721 ccagcaggtc aaacgcgggc agcaggtcgc tttgatgggc aataccgacg cttccagaac
      781 gcagcttcat ttcgaggtgc ggcaaaacgg caaaccggtt aacccgaaca gctatatcgc
      841 gttctgactt tctgcctgaa atcaagttgg aaatgccgtc tgaaaggttt tcagacggca
      901 tttttgtttt tggcggctgt gttttcaaat cattcggaag ggacggggca tataaatttg
      961 tttgactgtg tttgggtatt tgcttgattt catccgtttg cagtagaaac ggatggcgcg
     1021 atgtgcctgt ctttcagacg gcatagtccg ccgtcagcag ccgcaggaca tgtcttcaga
     1081 ggcttgcagg ctgatgtctc tgcgtttttt ggctttttcg tagcggcagc gttggcggtc
     1141 ggtcaggatt tccagcggag ggacagggac gggtttgccg tctttgactg caaccatggt
     1201 gaagtagcag ctgttggtat ggcggatttc tcccgtacgg atgttttgtg cttcgacacg
     1261 gatgccgatt tccatagagg tacgccccgt gtagtttacg ctggcgtaga aagtaaccag
     1321 gtcgccgaca tggatgggtt ctttaaacag gactttgtca accgacaggg taacgcaata
     1381 attgccgctg taacggctgg cgcaggaata ggcgacttgg tcgagcagga gcaggagttc
     1441 gccgccgtgt acgttgccgc tgaaattggc ggtgtccggc atcatcagtt cggacataat
     1501 gagttcgtgc gaaggcagtt ggcgttgttg tgtcatagtg tacctgtttt aagaaagggt
     1561 attttgaaat gggtgtggaa aaattttagg gaatgggaaa gtggaattca agctaaaaaa
     1621 agctatgatg ggatagcctg ccgaaatggg ctgccgatgc cgtctgaagg tttcagacgg
     1681 cattttgttt aggaaagcca aaaacggaag actaggaaaa acacgagtgc caatacgcct
     1741 gcccaaacga cgagcatgaa tccggcaact gcctgaccca tacggtaaac cgggtttttg
     1801 cgttcatctt caagaagtgg gttgtgattt ttctcggatt gttcggacat tttattctca
     1861 atcggaaaag ttggaaatgg aggtaaggtt acgccgtcat gcaaaaagat tcaaggattc
     1921 agttcagcag tcttcctccc agccgtcatc ccaggcatgt ttcaatgccg tctgaacggt
     1981 atcggcatca aaaccgcgat aggcgaggaa gcgtgcctgt ttttgttttt ctttaaggtc
     2041 ggcggccgga tgtttgaatt ttttacgcaa cacggctatg gcggccagtt tttcgcttga
     2101 gcggtcggga agcaggttgc ggctggtttc ttcatctatg ccctgttgcg ccaaagcctg
     2161 tttcagcctc aatgaaccgt gtttgcggct tttgctgcgg atataggctt cggcatagcg
     2221 caaatccgac tgccagttgc gttcggcaaa ttcgtttaac acgttttcca actcctcttc
     2281 gctttcggcg tgcggtgcaa gtttgcgttt cagaccgatg cggctgagtt cttggcgcga
     2341 gaggatgtcc atcgcgcggg cgcgtaggga tttttgcggt ttcatggcgg cggtttcggc
     2401 gtggggcagg tttcagacgg catagcggcg caaaccggag ccgtccgaat gcctactctt
     2461 ctttgttttt ctgaggttgc aggttttcgt aaatttcggg cagcgagccg ataatccagt
     2521 cggggcgggt cgcatcgtct tgcgagagca gcgtcatatc gccgtaaccg aaggtaacgc
     2581 cgacgctcag gcagccggcg gctttggcgg cgatgatgtc gttgcgcgag tcgccgacca
     2641 taaccatgtt tgcaacatcg atacccaaaa cttcggcggc gtgccgcagc ggcagggggc
     2701 tgggtttttt ctcgggcagg ctgtcgccgc cgagtatcag gctaaaatag tcggcgagtc
     2761 ccagttgttt tagaagctcg gaggcaagga tttcgttttt gttggtaacg acggcgagcg
     2821 ggatgcccaa agatttaagc aatgccagcc cggcttcggt ttcgggatag gggcgggtga
     2881 agacgctcaa atggtcgcgg tagtatttca tataggatac gaaacctttt tcccacagtt
     2941 cggaatcggc ttcgcggtcg cggtcgttgg tgaggacgcg gtgaaccagt ttgccgatgc
     3001 cgtcgcccac atagctttcg accactttgg caggcagcgg tttcataccg agttgttcca
     3061 acatcgcttc tgcggcggcg gcaaggtcgg ggacggaatc gcacagtgtg ccgtccaaat
     3121 cgaaggcgac ggcttggacg tgttcgatgg ctgcattcat aatccggttt cctgtcgggt
     3181 tgaaaaggtt tattttaaca tcgtttgcac agggcttcta atgtatggat tgttaacaat
     3241 cgaaacaggt tttgccggcg gtgtttccgg atttcggaac ggagtcggga agggtgtgca
     3301 aatcccggcg ccgttccaat taaattttta aaaaataaaa ggattcttat atctttattt
     3361 aattggacat ttgctttttg gatatgtgtt ccgctttttt cagacggatt ccgtgcggct
     3421 tgggaaataa aatcatatat agattaatca aatcgggccg attccaacta cacgctatta
     3481 cattttgctt gaaaattggt tatagttgcg aacctgatgg cttgttccat tccgaacctc
     3541 tctttgtgaa aaggaagcaa aatgtctgaa gcccttgcaa aagaaaccct caatccgttt
     3601 gaaatcgcgc gcaaacaggt gaaaaccgcc tgtgaccggc tgaaaaccga tccggccgtt
     3661 tatgaaatcc tgaagagtcc gacccgcgtg ttggaagtca attttcccgt caaactcgac
     3721 gacgacacgg tcaaaacctt cacgggctac cgttcgcaac acaacaacgc cgtcggcccc
     3781 tataaaggcg gcgtgcgctt ccatcccagt gtgaatttgg acgaagtcaa agccctgtca
     3841 atttggatga ccatcaaatg ctgcgttgcg ggtattcctt acggcggcgg caaaggcggc
     3901 attaccttgg atccgcgcga ttattccgag gcggaactgg aacgcatcgc ccgcgcttat
     3961 gctgaagcga ttgccccgct gattggcgag aaaatcgata ttcccgcccc cgatgtgaat
     4021 accaacggca aaattatgtc gtggatggtg gatgcttatg aaaacgtggt gaaacattcc
     4081 gcgccgggcg tatttaccgg aaaaccggtt gagttcggcg gctctttggc gcgtaccgag
     4141 gctaccggtt acggcgtgaa cctcgccgcc gtccaagctt tggaaaaact gggcaaggac
     4201 gtgaagggtg ccacttacgc cattcaaggt ttcggtaatg tgggttacca cacaggttat
     4261 tacgcgcatc aatccggcgc gaaagttgtt gcggtttcta ctgttgacgt tgccatttac
     4321 aacgaaaacg gtttggatat ggaagctttg tttaaagagt tccaagaaaa aggcttcatc
     4381 accaacgaag ccggttacgg caaagaaatt accaacgccg aacttttggc tttggatgtg
     4441 gacgtactcg ccccttgcgc gctggaaaac caactgactt ccgaaaacgc cggtaaagtc
     4501 cgcgcgaaaa tcgtagtcga aggcgcgaac ggcccgacta cgcccgaagc cgacgtgatc
     4561 ctgcgtcaaa acggcgtatt ggtcgtgcct gatattctgg cgaactgcgg cggcgtggtc
     4621 gtttcctatt tcgagtgggt gcaaaacctg caaggctatt attgggagtt tgacgaagtt
     4681 caagagaaag aaaccgtcgt cctgcgacgc gcgttccgcg atatatggaa cttggcgcaa
     4741 gagtatgatg tcgatttgcg aaccgcgtct tacatgatga gtatccgccg tgttgaaaaa
     4801 gcgatgaaac tgcgcggttg gtattgatgt ttgaacaaaa aatgccgtct gaagccttca
     4861 gacggcattt ttgtgcgggc gggattaatg gtgcgaatga ccgatttgga aggttaaagt
     4921 cgagtagttc gcctgttttt ggcacacgct ttgatcgggg aagtcggttt tgtgttcgac
     4981 attggctttc cagaagcctt ggcgcaaggg gatgatgtcc acttcgcctt tgtcgtctgt
     5041 gctgtcggag aaagcctgtg cttcggtttt gtgcgttttg ctgcggtcgc tggtgtcgaa
     5101 gccgtcaaag gtggcggtaa cggtggcatt gggcagcggt tcgccacgga acagaacgcg
     5161 gactttgaag cgttcgccta cgtgaatgtt ggcgggattg tccagcggga cgatttccaa
     5221 gttttgtccg accggtttgg tgatgatggc ggtgtccgcg ctttcgtgtc cgacgttgac
     5281 gatgtttttg ccgaacattc gggtttgttc gcaatagctt gcgtcaggca tttctttgat
     5341 gcccgcctgt ttccagcctg ctttgttttt tgaccagaaa gtaggctgat attcggcgat
     5401 gacgaggtaa ctgccgtcct taacgggacg gttgcttcgg tactggtagt tgtatgtgcc
     5461 gcgttgaatc atgttttcct tgcctttttc ggtaaccagc tgcatcggtt tgctgaagat
     5521 gtgcaggcgg tctttggcga tgggttcgag ttcgggaaat tcgccgtagc ccaagtcggc
     5581 tttaaggtat tcgccgccgt gcgtgtgggc ggtttcgacc cagacgcggt gggcgtgggc
     5641 ggatgtggca aatagggcgg aaacggcgag cagtgtcaat gttttcttca tggtgcagcc
     5701 tttgtctcgt gtgggttaaa gattttgtta tgttataaca aaataggtat cgtgtgaaga
     5761 cggatttgct gtctgaaaac tgtctttttg tatagtggat taacaaaaac cagtacggcg
     5821 ttgcctcgcc ttagctcaaa gagaacga
//
LOCUS       DAWIVB010000134         5838 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWIVB010000134 DAWIVB010000000
VERSION     DAWIVB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.134"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..2751
                     /locus_tag="WIX60_001629"
     CDS             <1..2751
                     /locus_tag="WIX60_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ5305873.1"
                     /translation="RLKNIYLLDKKNLARNHVQVINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKISSSAQAKNWSKPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKG
                     EDNLPVYHQRVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENHKALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLISSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(2790..5069)
                     /gene="clpA"
                     /locus_tag="WIX60_001630"
     CDS             complement(2790..5069)
                     /gene="clpA"
                     /locus_tag="WIX60_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ5305874.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNSDIPDALKDTEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPQAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTEVAKQ
                     LAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPVITKVVDKFLLQLEHRLLDKKVEAE
                     FTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDWDAAK
                     EEAVLKFKKSKVKIKTASA"
     gene            complement(5071..5373)
                     /gene="clpS"
                     /locus_tag="WIX60_001631"
     CDS             complement(5071..5373)
                     /gene="clpS"
                     /locus_tag="WIX60_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ5305875.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            5616..5819
                     /locus_tag="WIX60_001632"
     CDS             5616..5819
                     /locus_tag="WIX60_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ5305876.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
BASE COUNT         1412 a         1427 c         1616 g         1383 t
ORIGIN      
        1 cgtctgaaaa acatttatct gctggacaag aaaaaccttg cccgcaacca tgtgcaggtt
       61 atcaaccagt ttgagcagac gggcacgcat gcaaaccgct atgacgttac cgtgttggta
      121 aacggcctgc cgctggtgca gattgaattg aaaaagcgcg gtgtggcggt gcgcgaggca
      181 ttcaatcagg tgcaccgtta cagcaaagag agcttcaaca gcgaaaattc gctgttcaaa
      241 ttcctgcaaa tcttcgtgat ttccaacggc acggacacgc gctatttcgc caacaccacc
      301 aagcgcgaca aaaacagctt cgatttcacg atgaattggg cgcggtcgga caatcatccg
      361 attaaggatt tgaaagactt taccgccacg ttcctgcaga aaagcgtatt gctgggcgtt
      421 ttgctgcatt acagcgtgtt cgatgcgaat gatacgctgc tgattatgcg gccgtatcag
      481 attgccgccg ccgaacgcat tttgtggaaa atcagcagct cggcgcaggc gaagaattgg
      541 agcaaaccgg aaagcggcgg ctatgtctgg cacaccacgg gcagcggcaa aacgctgacc
      601 agctttaagg cggcgcgtct ggcgacggaa tcggcattta tcgacaaggt tttcttcgtg
      661 gtggacagga aggatttgga ctatcagacg atgaaggaat accaacgttt ttcgcccgac
      721 agcgtgaacg gttcggaaag cacggcaggc ttgaaacgca atttggaaaa agacgacaac
      781 aaaatcatcg ttaccaccat ccaaaagctg aacaacctga tgaagggcga agataatctg
      841 ccggtttacc atcagcgagt tgtctttatt ttcgacgaat gccaccgctc gcaattcggc
      901 gaagcgcaaa aaaacctgaa aaagaaattt aaaaaattct gccagttcgg ctttaccggc
      961 acgccgattt ttcccgaaaa cgctttgggc gcggaaacca cggcgggcgt gttcgggcgg
     1021 gagctgcatt cttatgtgat taccgatgcc atccgcgatg aaaaagtatt gaaattcaaa
     1081 gtggattaca acgacgtgcg cccgcagttc aaagccgtgg aagcggaaca ggacgagaag
     1141 aaactgagtg ccgccgaaaa ccacaaagcc ctgctgcacc ctgaacgcat ccgcgaaatc
     1201 acgcaatata tcctgaatca gttcaggcag aaaacgcacc ggctgaatgc gggtggcaaa
     1261 ggctttaacg cgatgtttgc cgtcagcagc gtggatgcgg cgaagtgcta ttacgaagcg
     1321 ttcaaaacac aacaggcagg cagcttgcac ccgctgaaag tggccaccat tttttccttt
     1381 gcggccaacg aagagcaaaa cgccgtcggt gaaattgtcg atgagacttt tgaaccggaa
     1441 gcgatggaca gcagcgcaaa agaatttttg caggctgcca tcaacgatta caacgcctgt
     1501 ttcaaaacca atttcggcac ggacagcaaa gcctttcaaa actactaccg agatttggca
     1561 aaacgggtga aaaatcagga aatagatttg ctgattgtgg tcggcatgtt tttgacgggt
     1621 tttgacgcgc cgacgctgaa cacgttgttc gtcgataaaa acctgcgcta tcacggcctg
     1681 atgcaggcgt tttcgcgcac caaccgcatt tacgatgcca ccaaaacctt cggcaatatt
     1741 gtctgcttcc gcgatttgga gcaggcaacc attgatgcga ttaccttgtt tggcgacaaa
     1801 aacaccaaaa acgtggtgct ggaaaaaagt tacgaagaat acatgaacgg ctataccgac
     1861 agccagaccg gcgaagcacg gcgcggttat ctggatgtgg caaaagaatt gcgcgagcgt
     1921 ttccccgatc ccgacaaaat cgaaacggaa aaagacaaaa aagattttgc caaactcttc
     1981 ggcgaatacc tgcgggcgga aaacgtattg cagaactacg atgaatttgc cgcgctgcgc
     2041 gagttgcaga gtgtggacgc ggcggacgaa gatgcgatga aggcgtttca agaaaaatac
     2101 tacctgagcg atgaagacgt gcaggaaatg cggcaagtgc cgatgccgtc tgaaagggcg
     2161 gtgcaggact accgttccgc ctacaatgac atccgcgact ggctgcgccg ccaaaaagca
     2221 ggcgaacaga aagagcaatc aaaaatcgac tgggacgatg tggtttttga ggtggatttg
     2281 ctcaaatcac aggaaatcaa tctggattac atcctgcaac tggttttcga acaccacaaa
     2341 aaaatcaaag gcaaagcgga gctggtggaa gaaatccgcc gcatcatccg cgccagcatc
     2401 ggccaccgcg ccaaagaggg tctgattgtg gatttcatca acgatacgga tttggacaaa
     2461 gtacccgacg ttcccgccat actggaaacc ttctacacct acgcgcaaga ggtgatgcgg
     2521 cacgaagcgg caggattgat tgccgccgaa ggcctgaacg aaaccgccgc caaacgctat
     2581 ttaatcagct cgctcaaacg cggctatgcc agcgaaaacg gcacggaact gaccgaaacc
     2641 ctgccgaaaa tgagtccgct caacccgcaa tatctgacga agaaacaaag tgtttttcaa
     2701 aagattgcgg cgtttgtgga gaagtttgcc ggaataggga ccgatatttg acaaaatgcc
     2761 gtctgaaatt tcagacggca tttttgattt tatgcggagg cggtttttat tttgaccttg
     2821 cttttcttaa acttcaacac ggcttcttct tttgccgcat cccagtctat ccgtacgaag
     2881 ccgccgtcgg atagtttgcc gaacaggagt tcgtcggcga gcggtttgcg gattttttcc
     2941 tgaatcaggc ggtgcatcgg gcgcgcgccc atttgcgggt caaaaccttt ttccgccaga
     3001 tatttgtgca atgccgacgt gaattcggct tcgacttttt tgtcgaggag ccggtgttcg
     3061 agctggagca ggaatttgtc cacgactttg gtgatgacgg gttcggataa gggcgcaaac
     3121 gggataatcg catccaagcg gttgcggaac tcgggcgtga agagcttgtt gatagcctgc
     3181 atttcgtcgc cgcgctcgcg tttggcggta aagccgaggc tgggtcggct gagactctcc
     3241 gcacctgcgt tagtggtcat aattaggatg acgttgcgga aatcggcact cttgccgttg
     3301 ttgtcggtca gtttgcctgc gtccatgact tgcaggagga cgttgaaaat gtcggggtgg
     3361 gctttttcga tttcgtccaa gagcaatacg cagtgcggct gtttgttgac ggcttcggtt
     3421 aaaaggccgc cttgttcaaa gccgacatag cccggaggcg cgccgatgag gcgcgatacg
     3481 gcatggcgtt ccatgtattc ggacatatca aagcgttgca gcggtacgcc catcgagtag
     3541 gcaagttgtt tggcgacttc ggttttaccg acgccggtcg gaccggagaa gaggaagctg
     3601 cctatcggtt tgtcgggcag tcccaagccc gaacgcgaca ttttgacggc ggcaaccagc
     3661 gcgtcgatgg cgttttcttg accgtaaacc atgtttttca aatcgcggcc gaggaattgc
     3721 agcacctgtt tgtcgtcgtg cgacacggtt ttttctggaa tccgcgcgac tttggcgatg
     3781 acggtttcga tttgcgcttt gccgatgact tttttctgtt tggatttggg cagaatccgt
     3841 tgcgccgcgc ctgcttcgtc catcacgtcg atggctttgt cgggcaggaa acgctcgttg
     3901 atgtagcgtg cggagagttc ggcggcggct tcgagtgcgc cttgcgtata gcggacttgg
     3961 tggaaggctt caaacatcgg cttcaagccg cgcaggattt gtacggtttc ggaaacggtg
     4021 ggttcgacca cgtcgatttt ttggaagcgg cggcttaagg catggtcttt gtcgaaaatg
     4081 gtgcggtatt cgtcgtaggt ggttgcgccg atgcagcgca gcgaaccttt tgccagcgcg
     4141 ggtttgagca ggttggacgc gtccatggtg ccgccgctgg tgctgcccgc gccgatgatg
     4201 gtgtggattt cgtcgataaa caaaatggct tgcgggattt tttcgagctg tttcaagacg
     4261 gatttgaccc gcgcttcaaa gtcgccgcgg tatttcgtgc ccgccaacag cgaacccata
     4321 tccagcgcgt acacttcggt atctttgagc gcgtcgggaa tgtcgctgtt gacgatttga
     4381 tgtgccaaac cttccgccag cgcggttttg cccacacctg cttcgccgac caaaagcgga
     4441 ttgtttttgc ggcggcggca caggatttgc accagccgtt ccatttcgtg tttgcgacca
     4501 atcaaagggt cgatacggcc ggctttgact tcggcgttga ggttgacggt gtagtcggaa
     4561 agggagccgg ttttttgttc cgcttcttcg ccctcgcgtc ccaggccgtc tgaatcattg
     4621 ccatcctcgt catcgggcga gccgtgggca atacagcgca aaacttcaaa acgcgtaacc
     4681 gattgcagct tgaggaaata gacggtgtgg ctgtcggttt cgctcatcag cgcgaccaaa
     4741 acgtccaacg gttcgactgc ggcttttccg gcagactggg tatgcaccat cgcccgttgc
     4801 atcacgcgtt ggaagccgag cgtgggccgt gtttcgaccg tgtctaaaag gtgttcggga
     4861 atcagggggg tgttttcggc aacgctggcg gcgagctgtt cggacaccac tttcaaatcc
     4921 gcgccgcata gttttaagac gttcggcacg gaggcatctt cttcgatgag taccaaaagc
     4981 agatgctcga ggctgataaa ttcataatga gccttacgcg cctcgcggta aagctgctgc
     5041 aaaatctgtt ccaattcggg tgcaagcata ttaaatctcc tcgacaatac attgcagcgg
     5101 atgcccttcg gcttttgccc gctgcatgac ttgttgctgt ttggtttggg caatatcgcg
     5161 cgtgtaagtg ccgcacaggc ctttgccttc gtgatgaacc aagagcatta ccgctaccgc
     5221 ctgttcttgt ccgagcatga agatttcggt caggatttca acgacaaatt ccatcgtggt
     5281 gtaatcgtcg ttcaatagga aaacgccgta acgtttcggc ggcagggtgt tcagacggtg
     5341 caagagcgtg tcggattggt gttgcgcggt catagtgtgt cccatttgaa agccgcgttc
     5401 agacggcatt tttgttgtat tttcggtact tttgcctatt ttcccacttt tttgaaaaca
     5461 tagcttgacg ttttgtctta acaaatgtaa aaagacggtt aagcaggatt gggcatccgc
     5521 cgagtatatc aagctggaag gaacgccaga cggctcggtt tgccgtatgc cttgttttgc
     5581 tgattttgtt aatttttgag tataggaagt ttctaatggc aaccggtatc gtaaaatggt
     5641 ttaacgacgc taaaggtttt ggtttcatca ctcctgatga aggcggcgaa gatttgttcg
     5701 ctcacttctc agcaatcaat atggaaggtt tcaaaaccct gaaagaaggc caacgcgtct
     5761 ctttcgacgt aaccaccggc cctaaaggca aacaggccgc caacattcag gctgcttaat
     5821 tcctgatgta cggtcaaa
//
LOCUS       DAWIVB010000135         5837 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWIVB010000135 DAWIVB010000000
VERSION     DAWIVB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.135"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            284..1231
                     /locus_tag="WIX60_001633"
     CDS             284..1231
                     /locus_tag="WIX60_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ5305877.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1384..2508)
                     /gene="rluD"
                     /locus_tag="WIX60_001634"
     CDS             complement(1384..2508)
                     /gene="rluD"
                     /locus_tag="WIX60_001634"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ5305878.1"
                     /translation="MQNTSFDNESDYSDDSDFVSAPETENCICLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2507..3310
                     /locus_tag="WIX60_001635"
     CDS             2507..3310
                     /locus_tag="WIX60_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ5305879.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLKQPWRSDDMPWWRYWH"
     gene            complement(3538..5757)
                     /locus_tag="WIX60_001636"
     CDS             complement(3538..5757)
                     /locus_tag="WIX60_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ5305880.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTAVRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTASAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFRAVRNIPA
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1402 a         1859 c         1400 g         1176 t
ORIGIN      
        1 ttctccgagc tttatatgtt tcaacagagg acggcacatc aagcaccgca ctgcgtgttg
       61 cccgaacgag actaccctcc gattagattc tatcgccata aacagacggg tttcaaccga
      121 aaaggaatgg gaataaagtc catttccgac acctctcggg cgatgccgtc tgaaaaccaa
      181 tctccacttt cagacggcat tgtttaggca cggtcatttt gcaaattaag gttaaggcgt
      241 gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt attatgaata tcctcagtaa
      301 aatcagcagc tttatcggaa aaacattttc cctctgggcc gcgctctttg ccgccgccgc
      361 ttttttcgcg cccgacacct tcaaatgggc ggggccttat attccttggc tgttgggcat
      421 tattatgttc ggtatgggtt tgacgctcaa accttccgac ttcgatattt tgttcaaaca
      481 tcccaaagcc gtcatcatcg gcgtaatcgc acaattcgcc attatgccgg caaccgcctg
      541 gctgctgtcc aaactgttga acctgcctgc cgaaatcgcg gtcggcgtga ttttggtcgg
      601 ctgctgcccg ggcggtacgg cttccaatgt gatggcctat ctggcgcgcg gcaatgtggc
      661 tttgtcggtt gccgttacgt ctgtttccac cctgatttcc ccattgctga ctcccgccat
      721 cttcctgatg cttgccggtg aaatgctgga aatccaagcg gccggtatgt tgatgtccat
      781 cgtcaaaatg gttttgctcc ccattgtttt gggtttgatt gtccataagg ttttgggcag
      841 taaaaccgaa aagctgaccg atgcgctgcc gctggtttcc gttgccgcca tcgtgctgat
      901 tatcggcgcg gttgttgggg caagcaaagg caagattatg gaaagcggcc tgctgatttt
      961 tgcggttgtc gtactccaca acggcatcgg ctacctgctc ggcttctttg ccgccaaatg
     1021 gaccggcctg ccttatgatg cacaaaaaac gctggccatc gaagtcggta tgcaaaactc
     1081 gggcctggcc gccgcgcttg ccgccgcaca ctttgccgct gcgccggtcg ttgccgttcc
     1141 gggcgcattg ttcagcgtgt ggcacaatat ctccggctcg ctgctggcaa cttattgggc
     1201 ggccaaagcc ggtaaacata aaaaacccta aacgcctttc atcataatgc cgtctgaacg
     1261 gggcaaacga aaccggcggt ttctgcccga ccccttcaga cggcctttgt ttgaaatcgg
     1321 cagggacgat tgtccggctg ctccagttcg gctgcctgcg ttcgccccgc ctttcaagcc
     1381 gcctcactcc cttacataaa ccacttcgac atcgtagtcg tcttcgttcc aatcgtcatc
     1441 gtcgtccgcg ccgaatttgt cctgccattc ctcttcattg ctcaaagacg aatccaaacc
     1501 ggcttcaaga cggaggacag acaacaaatg atatatgtcg tccggaatcg gtgcttcaaa
     1561 cgaaacggtt tcgccggttt tcggatgagt gaaacttaag cggtaggcgt gcaatgcctg
     1621 acgcgcaccc aaacttttaa cggcttcttt caccgtgtcg ccgcacggat ggcgcgggtt
     1681 gccgtaaacc gggtcggcgg caagcggatg gttggcctca cgcatatgga cgcggatttg
     1741 gtgcgtcctg cctgtttcga gcgagcattc gatatagctg tgcgccagat agcgttccaa
     1801 cactttgacg tgggtaacgg ctggtttgcc gccgaatttg acgactgcca ttttcaggcg
     1861 gttgtgcgga tcgcgtccga tttgggtttc gattttaccg tcaaagggaa cgatgccgtt
     1921 ggcgacggcg cggtaaatgc gtttgaccgt gcgctcttga agctgttgca cgagggaatt
     1981 ttgcgccggc agggttttgg caaccaccat cagcccgctg gtttctttgt ccaaacggtg
     2041 tacgatgcct gcacgaggta cttggctcaa ttcgggacaa tgcgccaaga ggccgttgag
     2101 cagcgttccc gtccagttgc ccgccgccgg atgcaccacc agtccggccg gtttgttgac
     2161 gacgatgacg gtatcgtctt cgtaaacaat atccaaagcc ataggctctg gaacaaacgc
     2221 cagattttcc tcactcggac ggacggttac acaaatttgc tcgccgccta tcattttgtc
     2281 tttgggttgc gaaggtttat cgtttacaat aaccgcgcct tctttaatcc atgatgtcag
     2341 gcggctgcgc gagtagtcgg gcagaagttt cgccaacacc gcatccagcc gcccgccggc
     2401 aagctcgagc ggaacagtca gacaaataca gttttcggtt tccggggctg acacaaagtc
     2461 tgaatcatcg ctataatcgg attcattatc aaaggaagta ttctgcatga aaaaaattct
     2521 tttaacggtt tcattaggtt tggcgctgag tgcctgtgcc actcaaggta cggtagataa
     2581 agatgcccag attacccaag actggagtgt ggaaaaactc tatgccgaag cccaggatga
     2641 gctgaacagc agcaattata ctcgagctgt caaattatac gaaatcttgg aatcgcgctt
     2701 ccccaccagc cgccatgccc ggcagtcgca actggatacc gcatacgcct attataaaga
     2761 cgatgaaaaa gacaaagctc tggcggcaat cgaacgcttc cgccgccttc accctcaaca
     2821 cccgaatatg gattacgcgc tgtatctgcg cggcttggtg ctgttcaacg aagatcagtc
     2881 tttcttgaac aaactggcct cgcaagactg gtccgaccgc gacccgaaag ccaaccgcga
     2941 agcgtaccag gcgtttgcgg aactcgtcca acgcttcccg aacagcaaat acgccgccga
     3001 tgcaaccgca cgcatggtca aactggtcga tgcactgggc ggcaatgaaa tgtcggtggc
     3061 gcgctactac atgaaacgcg gtgcatacat cgccgccgcc aaccgcgccc aaaaaatcat
     3121 cggcagctac cagaatacgc gttatgtcga agaatcgctc gccatattgg aacttgccta
     3181 ccaaaaactc ggcaaaccac agcttgccgc cgatacgcgc cgcgtgttgg aaaccaactt
     3241 cccgaaaagc ccgttcttaa agcagccgtg gcgatctgac gatatgccct ggtggcgtta
     3301 ctggcactga ttccgcaccg atgtccggat gccgtctgaa gccctttcag acggcatttc
     3361 cccttctggc gcaaaccttt ccgttcattc aaacggttct ccctgccgtt aaccgcaacg
     3421 ccctttctgt ttttgacgca cactgaaaat caaacgatat gccttgccag cacgaaccgg
     3481 cgttaacgcc aaaatgccgt ctgaatccgt ttcagacggc attttatgtt tcaaacctca
     3541 ctcaaacggt tttttctgcc aatagaattt ataccctctc aaacgttgag ccttcacgcc
     3601 gccgcgtccc aagccgaatt gcagcgcacc cgacaaatcg gtacgcagca gtttaatgcc
     3661 gtgtgcgcgg acacggttct gcaccgcttc ggtcggatgt ttgtaggcgt tggcataacc
     3721 gcttgaagca acggcatatt cgggcgaaac ggcattgagg aacacgcccg acgaggacgt
     3781 attgctgccg tgatgcccca acaccaacac ctggctgtac aggttgcctc catacttgcc
     3841 gaccaggctt tcctcgccct tcgtatccaa atcgcccgtt accagcagtg ccgcaccgcc
     3901 cgccacaaca cgcaaaacac aacttttccc attatcatcg atgtttttgc gttcagacgg
     3961 cctcaaaaac tcgaaatcta cgccgtccca ttgccaacgc tgttccgcac aatgccgcgc
     4021 cccctcataa aattccggct gcccggcata aatcccgccg gcgggaatat tcctcaccgc
     4081 ccgaaaaccg ccgtcgtggt cgctgtcgtg atgcgacaga accagcttgt ccaaacggcg
     4141 gacacccgcc gcattcaaac tcggcacaat ccccgtctgt gccgccgatg cagttccagt
     4201 gtcaaacaaa agatgatgat ttgccgtctg aaccgacacc gacaaaccct gccccgcatc
     4261 ccaaaccgta accgcaacct cattttccgg cacgccgggt gaacggtaaa acacaaaccc
     4321 tgccaacagc aacaccgccc acggacgcaa acccaagccg cgcggcagca gcaacagcaa
     4381 agcggcacac accgccaaca ccaacagcgg caaaggtgcg gcggcaacgg caaactcggg
     4441 cgacacatcg gcaagccaca ccaaaaaccg caaagtatat tccgccaaaa atgcccccaa
     4501 ctgttgcaaa ggcgcaaacg gcacgaccga acccagcaac gccagcggcg tcaataccca
     4561 agaaaaccac ggaatcgcca ccgcattgac caaagggctg attaaaggca gcgaagcaaa
     4621 cagataaccg agcaaaacca gcgacaacac cgaagccgcc cactgcccgc gcacggcggt
     4681 ttgccgtttc ccttcgtgca aacgccccga acacgcccat atcagggccg ccaccaaacc
     4741 gaaagacagc caagtcccca cacccaagac cgccaaaggg tcgaacagca gcactgccgc
     4801 caacgcctgc caccacgtcg cccacgccga caatcttccc ctgcgccaag cccacgcaaa
     4861 cgccgccaac atcaaaacgc tgcgctgcgt cggcacggaa aaaccggcaa gcagcgcgta
     4921 aaacagcgcg cctgcacacc ccgccgccaa aacccacacg cgcggccggg caggaaggcg
     4981 cggggaacag gcaagcagcc gcttcgccag ccacgcaaac atcaccgcca ccatcgtaac
     5041 gtgcaaaccc gaaatactga ccaaatgcgt cagccccaac ggtcggaacg cctgccacaa
     5101 ttcggggcgc aatgccgact gttcgcccac gctcaacgcg cgcatcagcc cgatgccgtc
     5161 tgaaagcccg ccgtccgcat ccgcctgctg ccaattacgg ctgatgcggc tgcgccaaac
     5221 cgcaatcccc caaccgcttc cgccatgcag caaaaccctg tccgcaccga ccgtccccgc
     5281 gccgcctatc ccgttggata atgcccacgc ctcacggttc aaaccgcgca ggttcaattc
     5341 gccgacgacg gggtgcacac gtgccgttat ccgccatctg ctcccgaccg cccattcgcg
     5401 ccgtttgtag tccgacagca gcaaatcaaa cgtccgacca ccgctgtcca cagcctttgc
     5461 cgcaaactgc acgcgccgcc cgtccgacct cggcatatcc gccacttcca ccgtcaacgg
     5521 cacacctgaa accgcctccg cccgccattg cgaagacagt gccgcttccg ttctgaatac
     5581 gccgtaagcc gcgcccgcca acacgcacag catcagaccg gcaaacgcaa aacgccttgc
     5641 aaacacagca aaaaccgcaa aagccgccag ccaaaacagc caatgcggca cgaccggcag
     5701 cgcaaacgaa gccgccgcgc cgacaaccca acacggcaaa aaccaacgac ggaacacgtc
     5761 tccccacccc agatatttca aaaataaacg gataataaag caaatccgaa aaaccgtcat
     5821 aacaagattt gaaaatg
//
LOCUS       DAWIVB010000136         5749 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWIVB010000136 DAWIVB010000000
VERSION     DAWIVB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.136"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            244..5694
                     /gene="iga"
                     /locus_tag="WIX60_001637"
     CDS             244..5694
                     /gene="iga"
                     /locus_tag="WIX60_001637"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ5305881.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGNGEHHWNITFG
                     TNSKIGSTAVRLAGNEKDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGIN
                     NDITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVI
                     LNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFE
                     HIRNVDEGARIVNHNTGHASTITLTGKSLITDPKTISIHYIQNNDDDDAGYYYYRPRK
                     PIPQGKDLYFKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKN
                     NQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLS
                     GRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNV
                     SDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGN
                     VNLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNN
                     ASDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGE
                     PNQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRV
                     KPAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKR
                     QQAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQ
                     KVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQ
                     SQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAEL
                     LAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQA
                     LAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAE
                     LARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQD
                     YMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDE
                     NVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELH
                     EKVETVSLQPRAAQPRAQAATQLQAQAAAQADAVSTNTNSALSDAMASTQSILLDTGA
                     SLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQ
                     IGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQK
                     ANFNRASIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTL
                     TAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALL
                     YRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1939 a         1372 c         1287 g         1151 t
ORIGIN      
        1 caaaagcggg aatccaaaaa caaaaagccg cagaaattta acggaagaac cgaaccccga
       61 aaatcggctt cccgccttcg cttgaataac agaacaaagg aagcggaaat aaaaacatcg
      121 ggattctttt tttaaattgg caataaaaca ccaaaatgaa tgatttacac aacaaaaaat
      181 agtcaacacc accaaaccgg cgtaaaatac aaaacattat cacccttaaa acggtaaaac
      241 cttatgaaaa ccaaacgttt taaaattaac gccatatcct tatccatctt tcttgcctat
      301 gcccttacgc catactcaga agcggcattg gtcagagacg atgtcgatta tcaaatattc
      361 cgtgactttg cagaaaacaa aggcaaattt tttgtcggcg caaccgattt atcagtgaaa
      421 aacaaacaag gtcaaaacat cggcaacgca ttatccaacg taccgatgat tgattttagc
      481 gttgcagatg tcaacagacg tacattaacc gtcatcgatc cccagtatgc cgtcagcgtc
      541 aaacatgtaa aaggagacga aatctcttat tacgggcatc acaatgggca cttagatgtt
      601 agcaatgatg aaaacgaata tcgttcagtt gcacaaaatg actacgaacc aaataaaaat
      661 tggcatcacg gtaatcaagg tcgtcttgaa gactacaaca tggcacgcct taataaattc
      721 gtaacagaag tcgcacctat tgcaccaacc agtgcaggcg gcggcgttga aacctataaa
      781 gataaaaacc gtttttctga gtttgtgcga gttggagcag gtacgcaatt tgaatataac
      841 agccgctaca atatgacaga gctttcacga gcttatcgtt atgccattgc aggtacgcct
      901 tatcaagacg tgaatgtcac atcgaatcta aatcaagaag gcttgattgg cttcggtgat
      961 aattcaaaac atcattcccc agaaaaactc aaagaagttt tatctcaaaa cgcattgact
     1021 aactatgctg tgttaggcga tagcggttca ccattatttg cttatgataa acaagaaaaa
     1081 cgctgggtct ttttaggtgc ttacgattat tgggcaggct atcaaaaaaa ctcttggcaa
     1141 gaatggaata tctataaaaa agaatttgca gataaaatca aacaacgcga taacgccggc
     1201 accatcaaag gtaatggaga acatcattgg aacattactt ttggcacaaa cagcaagatt
     1261 ggttctacag cggtaaggct tgccggcaat gaaaaggatg caaacaacgg acagaatgtt
     1321 acctttgaag acaacggcac tttggtattg gatcaaaaca tcaaccaagg cgcgggcggt
     1381 ctgtttttca aaggcgatta cacagtcaaa ggtataaata atgacatcac ttggctaggt
     1441 gcggggattg atgttaccga cggcaaaaaa gtcgtttggc aagtcaaaaa tccgaatggc
     1501 gacagattgg caaaaatcgg caaaggcaca ttagaaataa acggcacagg cgttaaccaa
     1561 gggcaattaa aagtcggcga cggtacggtt attctgaatc aacaagccga tgccgacaaa
     1621 aaagtccagg ctttctccca agtcggcatt gtcagcggac gcggtacatt ggtattaaat
     1681 agttcaaatc agattaatcc cgataaccta tatttcggtt tccgtggcgg tcgtttggat
     1741 gccaatggca atgacttgac ttttgaacac atccgcaacg tggatgaagg cgcgcgcatt
     1801 gtcaaccaca acacaggcca cgcctccaca atcacgctaa cgggtaaatc tttgattacc
     1861 gatcccaaaa ccatctctat tcattatatt caaaataatg atgatgacga tgctggttat
     1921 tactattacc gccctaggaa accgattcca caaggcaaag atctttattt caaaaactac
     1981 cgttattacg ccctaaaatc cggcggcagc gtgaacgcac cgatgcccga aaacggacaa
     2041 acggaaaata acgactggat tttaatgggc agcacacaag aagaagccaa gaaaaacgcg
     2101 atgaaccaca aaaacaatca gcgtattagc ggttttagcg gtttctttgg cgaagaaaac
     2161 ggaaaaggac ataacggtgc attaaacctt aatttcaacg gcaaaagcgc gcaaaaccgt
     2221 ttcttgttaa caggcggcac taatttaaac ggaaaaataa gcgtaactca aggcaatgtc
     2281 ttgttatcag gtcgtccaac accacacgca agagattttg tgaacaaatc ttcagcccga
     2341 aaagacgcac atttctccaa aaacaatgaa gtcgtatttg aagacgactg gataaaccgc
     2401 acattcaaag ccacagaaat tgcggttaat caatccgcat cattctcttc cggcagaaat
     2461 gtatccgaca tcaccgccaa catcaccgca acagacaacg ccaaagtaaa tttgggttac
     2521 aaaaacggcg atgaggtttg cgtgcgctcg gactataccg gttacgttac ctgcaacaca
     2581 ggcaacttat ccgataaggc tttaaacagc tttggagcga cacagattaa cggcaatgtg
     2641 aatctgaacc agaatgcggc attggtcttg ggcaaggctg cattatgggg gcaaattcaa
     2701 gggcaaggaa acagccgtgt cagcctaaac caacatagca aatggcattt gaccggcgac
     2761 agccaagtac acaatctgtc attggcggat agccatattc atttgaacaa tgcctccgat
     2821 gcgcaaagtg caaataaata ccacacgctc aaaatcaatc atttatccgg taacgggcat
     2881 tttcattatc tgacgcacct ggcgaaaaat cttggggata aagtgcttgt gaaggaatcc
     2941 gcatccggtc attatcagct ccatgttcaa gataaaacag gcgaacccaa tcaggaaggg
     3001 ctgaatctct ttgatgcatc atccgtacaa gaccgctccc gcctttctgt ttccttggcg
     3061 aataaccatg tagatttagg ggcattgcgt tatacaatca aaacagaaaa cggtattacc
     3121 cgattgtaca atccttatgc cgagaaccgc cgccgagtca aaccggcccc gtctcctgcc
     3181 acaaacacgg cttctcaagc acaaaaggca acacaaacgg acggtgcaca aattgccaag
     3241 cctcaaaata tcgtcgtcgc accgcctagc ccgcaggcaa atcaagccga agaagcaaaa
     3301 cgccagcaag caaaagcgga gcaagtgaag cgtcagcaag cagaagcaga gcgtaaatcg
     3361 gcagagttag caaaacaaaa agcagaagcc gaacgcgaag caagagagct ggcgacccgc
     3421 caaaaagcag aacaagaacg cagcagcgcc gaacttgcac gtcgacatga aaaagaacgc
     3481 gaagcggcag agttgtcggc aaaacaaaaa gtcgaagccg aacgtgaggc tcaagcattg
     3541 gcggtacgcc gaaaagcaga agccgaagag gcaaaacgcc aagctgcaga gcttgcacgc
     3601 cggcatgaaa aagaacgcga agcggcagag ttgtcggcaa aacaaagggt cggggaagag
     3661 gaacgccgtc aaacagccca atcccagccg caacgccgca aacgccgtgc cgcaccgcag
     3721 gattatatgg cagcttccca agaccgtcct aaacgccgcg gacaccgttc ggtacagcaa
     3781 aacaatgtgg aaattgccca agcccaagca gaacttgcgc gccgacagca ggaagaacgc
     3841 aaagcggcag agttgttggc aaaacaaagg gcagaagccg aacgtgaagc tcaagcattg
     3901 gctgcgcgcc gaaaagcaga agccgaagag gcaaaacgcc aagctgccga acttgcacat
     3961 cgccaggaag cagaacgcaa agcggcagaa ttgtcggcaa accaaaaagc cgcagctgaa
     4021 gcccaagcat tggcggcacg ccaacaaaaa gcccttgcgc gtcaacagga agaagcacga
     4081 aaagctgcag agttagccgt caaacaaaaa gcggaaaccg aacgcaaaac cgccgaactt
     4141 gccaagcaaa gggcagcagc cgaagcagca aaacgccagc aagaagctcg ccagaccgcc
     4201 gaacttgcac gtcgtcaaga agcggaacgc caagcagcag agttgtcggc aaaacaaaaa
     4261 gccgaaacag atcgagaagc ggcagaatcg gcgaaacgaa aagctgagga agaagagcac
     4321 cgtcaagcag cccaatccca gccgcaacgc cgaaaacgcc gtgccgcacc tcaggattat
     4381 atggcagctt cccaaaatcg tccgaaacgt cgcggacgca gatctactct gccggcaccg
     4441 ccctcgccat catttgattc gtcagcttac gcagcaccca gggccttgca taatccggac
     4501 tggtatgaaa atgactatga agaaatcccc ttggatgcgt tggaagatga aaatgtatct
     4561 gaatcagttg acacatcaga caaacagcct caagacaata cggaacttca tgaaaaatat
     4621 gaaaatgatt atgaagaaat ccccttggat gcgttggaag atgaagatgt atccgaatcg
     4681 gttgacacat cagacaaaca gcctcaagac aatacggaac ttcatgaaaa agttgagaca
     4741 gtgagtttgc aaccaagagc cgcgcagccg cgagcccaag ccgccactca gctgcaagcc
     4801 caagccgccg cgcaagccga tgcagtcagc accaatacca actcggcttt atctgacgca
     4861 atggcaagca cgcaatctat cttgttggat acaggtgctt cattaacacg gcacattgca
     4921 caaaaatcac gcgctgatgc cgaaaaaaac agtgtttgga tgtcgaacac cggttatggt
     4981 cgtgattatg cttccgcaca atatcgccgg tttagttcga aacgcacgca aacacaaatc
     5041 ggcattgacc gcagcttgtc cgaaaatatg cagataggcg gcgtattgac ttactctgac
     5101 agtcagcata cttttgatca ggcgagcggc aaaaatactt ttgtgcaagc caacctttat
     5161 ggtaagtatt atttaaatga tgcttggtat gtggccggcg atattggtgc aggcagcttg
     5221 agaagccggt tacaaacgca gcaaaaagca aactttaacc gagcaagcat ccaaaccggc
     5281 cttactttgg gcaatacgct gaaaatcaat caattcgaga ttgtccccag tgcggggatc
     5341 cgttacagcc gcctgtcatc tgcagattac aagttgggta acgacagtgt taaagtaagt
     5401 tctatgtcag tgaaaacact aacggccgga ctggattttg cttatcggtt taaagtcggc
     5461 aaccttaccg taaaaccctt gttatctgca gcttactttg ccaattatgg caaaggcggc
     5521 gtgaatgtgg gcggtaattc cttcgtctat aaagcagata atcaacagca atattcagca
     5581 ggtgccgcgt tactgtaccg taatgttaca ttaaacgtaa atggcagtat tacaaaagga
     5641 aaacaattgg aaaaacaaaa atccggacaa attaaaatac agattcgttt ctaaaatact
     5701 aaattcatag caaaataaaa tgccgtctga actcaggctt cagacggca
//
LOCUS       DAWIVB010000137         5708 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWIVB010000137 DAWIVB010000000
VERSION     DAWIVB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.137"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            72..998
                     /gene="fabD"
                     /locus_tag="WIX60_001638"
     CDS             72..998
                     /gene="fabD"
                     /locus_tag="WIX60_001638"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ5305882.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKAICAEAAQSEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            1136..3001
                     /gene="msbA"
                     /locus_tag="WIX60_001639"
     CDS             1136..3001
                     /gene="msbA"
                     /locus_tag="WIX60_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ5305883.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLFALQTANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3101..4666
                     /gene="guaA"
                     /locus_tag="WIX60_001640"
     CDS             3101..4666
                     /gene="guaA"
                     /locus_tag="WIX60_001640"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ5305884.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNESKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVIALRAVITSDFMTAHWAELPYSLLGKVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4728..5474
                     /gene="fabG"
                     /locus_tag="WIX60_001641"
     CDS             4728..5474
                     /gene="fabG"
                     /locus_tag="WIX60_001641"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ5305885.1"
                     /translation="MSTQDLNGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETIESLIADIEKAFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1387 a         1818 c         1376 g         1127 t
ORIGIN      
        1 ttttgtcccg cctgaatttt tcgtaaaact cgggcagaat acctgattat ccaaccaaac
       61 aaaggaatac tatgtctttt gccttctttt ttcccggaca aggttcccaa agcctcggta
      121 tgatgaacgg ctttgccgaa cacgccatcg tcaaaaacac ctttgccgaa gcctccgcca
      181 tattggggca ggacttgtgg gcgatgataa acggcagcga tgccgaaatc atcggtcaaa
      241 ccgtcaacac ccagcccatt atgctcgccg ccggcgttgc cgtttaccgc gcctatttag
      301 aagcgggcgg caaaacgcct gccgccgttg ccggacacag cctcggcgaa tacaccgcac
      361 tcgttgccgc cggcgcattg aattttgccg acgcggtcaa actcgtgcgc ctgcgcgccg
      421 aactgatgca gtccgccgta ccgcaaggcg tgggcgcaat ggcggcgatt ctcggcttgg
      481 aagatgagca ggttaaagcc atttgtgccg aagccgccca aagcgaagtg gtcgaagccg
      541 tcaacttcaa ctcacccgga caaatcgtga ttgcaggcaa cgccgccgcc gtcggacgcg
      601 ccatggctgc cgccaaagaa gccggtgcca aacgcgccct gccgctgccc gtgtccgtac
      661 cttcccattg cagcctgatg aaacccgccg ccgacaaact tgccgaagcc ctgaaaaccg
      721 ttgaaatcaa gcagccgcaa atccgcgtta tccacaacgc cgacgttgcc gcctacgatg
      781 atgccgacaa aatcaaagac gcgctcgtcc gccagcttta cagccccgta cgctggacgg
      841 aaaccgtcaa cgccctcgtt tcagacggca ttgccgaatc cgccgaatgc ggcccgggca
      901 aagtgttggc gggcttggca aaacgcatca acaaagccgc cgcgtgcagc gcactgaccg
      961 atgccggaca ggttgccgcc tttatcgaag cgcactgact tcgttctgca aaaagcagcc
     1021 tgccctcttc aggctgcttt tcatgtccga acgacggcag ccccatattt acgctataat
     1081 ccatcccgac caaaccaccg acagcggctg ccgttgcagt tcccgcccta ccgatatgat
     1141 agaaaaactg actttcggac tgtttaaaaa agaagacgcg cgcagcttta tgcgcctgat
     1201 ggcgtacgtc cgcccctaca aaatccgcat cgttgccgcc ctgattgcca ttttcggcgt
     1261 tgccgccacc gaaagctacc ttgccgcctt catcgccccc ctgattaacc acggcttttc
     1321 cgcacctgcc gcgccgcccg agctgtctgc cgccgccggc atcatttcca ccctgcaaaa
     1381 ctggcgcgaa cagtttacct atatggtttg ggggacggaa aacaaaatct ggaccgtccc
     1441 gctcttcctc atcatcctcg tcgtcatccg tggcatctgc cgctttacca gcacctatct
     1501 gatgacttgg gtctccgtga tgaccatcag caaaatccgc aaagatatgt ttgccaaaat
     1561 gctgaccctt tcctcccgct accatcagga aacgccgtcc ggcaccgtac tgatgaatat
     1621 gctcaacctg accgaacagt cggtcagcaa cgccagcgac atcttcaccg tcctcacgcg
     1681 cgacacgatg atcgttaccg gcctgaccat cgtcctgctt tacctcaact ggcagctcag
     1741 cctcatcgtc gtcctgatgt tccccctgct ctccctgctc tcgcgctact accgcgaccg
     1801 tctgaaacac gtcatttccg actcgcaaaa aagcataggc acgatgaaca acgtgattgc
     1861 cgaaacccat cagggacacc gcgtcgtcaa gctgttcaac gggcaggcgc aggcggcaaa
     1921 ccggttcgac gcggtcaacc gcaccatcgt ccgcctcagc aaaaaaatca cgcaggcaac
     1981 ggcggcacat tccccgttca gcgaactgat cgcctcgatc gccctcgccg tcgtcatctt
     2041 catcgccctg tggcaaagcc aaaacggcta caccaccatc ggcgaattta tggcattcat
     2101 cgtcgcgatg ctgcaaatgt acgcccccat caaaagcctt gccaacatca gcatccctat
     2161 gcagacgatg ttcctcgccg ccgacggtgt atgtgcattt ctcgacaccc cgcccgaaca
     2221 ggacaagggc acgctcgcac cgcagcgtgt cgaagggcgc atcagcttcc gcaacgtcga
     2281 tgtcgaatac cgttcagacg gcatcaaagc cctcgacaac ttcaacctcg acatcagaca
     2341 aggcgaacgc gtcgccctgg tcggacgttc cggcagcggc aaatccaccg tcgtcaacct
     2401 gctgccccgc tttgtcgaac cgtctgccgg caacatctgc atagacggta tcgacatcgc
     2461 cgacatcaaa ctcgactgcc tgcgcgccca attcgccctc gtctcccaag acgtattcct
     2521 gtttgacgac accctgtttg aaaacgtccg atacagccgt cccgacgcgg gcgaagccga
     2581 agtcctgttc gccctccaaa ccgccaacct gcaaagcctg attgacagct ccccgctcgg
     2641 actgcaccag cccatcggat cgaacggcag caacttatcc ggcggacagc ggcaacgcgt
     2701 cgccattgcc cgcgccattt tgaaagacgc gccgatatta ttattggacg aagccaccag
     2761 cgcattagac aacgaatccg aacgcctcgt ccaacaggcg ctcgaacgcc tgatggaaaa
     2821 ccgcaccggc atcatcgtcg cccaccgcct gaccaccatc gaaggggccg accgcatcat
     2881 cgtgatggac gacggcaaaa tcatcgaaca aggcacacac gaacaactga tgtcccaaaa
     2941 cggttactac acgatgttac gcaatatctc aaacaaagat gccgccgtcc ggacggcata
     3001 aacaaaatgc cgtccgaaat ggtacaatcg ccccgaccct ttcagacggc atcatatccg
     3061 ccgacccatc cgattatctt caatcactgt aaaacccatt atgacccaag acaaaatcct
     3121 catccttgac ttcggttcgc aagttaccca gctcatcgcc cgccgcgtgc gcgaagccca
     3181 cgtttactgc gagctgcatt ctttcgatat gcctttggac gaaatcaaag ccttcaaccc
     3241 caaaggcatc attctctccg gcggccccaa ttccgtttac gaatccgact atcaagccga
     3301 taccggtatt tttgatttgg gcattccggt tttgggcatc tgttacggca tgcagtttat
     3361 ggcgcaccac ttgggcggcg aagtgcagcc cggcaaccag cgcgaattcg gttatgcgca
     3421 agttaaaacc atagacagcg agctgacacg cggcattcaa gatggtgagc caaacacact
     3481 cgacgtatgg atgagccacg gcgacaaagt gtccaaactg cccgacggtt tcgccgtcat
     3541 cggcaacacc ccgtcctgcc cgattgccat gatggaaaac gccgaaaaac aattctacgg
     3601 catccagttc caccccgaag ttacccacac caaacaaggc cgcgccctgt tgaaccgctt
     3661 tgtcttggat atttgcggcg cacaaccggg ctggacgatg ccgaactaca tcgaagaagc
     3721 cgttgccaaa atccgcgaac aggtcggcag cgacgaagtg attttaggtc tgtccggcgg
     3781 cgtggactct tccgtagccg ccgcgctgat tcaccgcgcc atcggcgacc aactgacctg
     3841 cgtgttcgtc gatcacggtt tgttgcgcct gaacgaaagc aaaatggtga tggatatgtt
     3901 cgcccgcaac ttgggtgtga aagtgataca cgtcgatgcc gaagggcagt ttatggcgaa
     3961 actcgccggc gtaaccgacc ccgagaaaaa acgcaaaatc atcggtgcgg aatttatcga
     4021 agtatttgat gccgaagaaa aaaaacttac caacgccaaa tggttggcac aaggcacgat
     4081 ttaccctgac gtaatcgaat ccgcaggtgc aaaaaccaaa aaagcccacg ccatcaaatc
     4141 gcaccacaac gtcggcggcc tgcctgaaaa catgaagctc aaattgcttg agcctttgcg
     4201 cgatttgttc aaagacgaag tacgcgaatt gggtgtggct ttgggcctgc cgcgcgaaat
     4261 ggtgtaccgt catccgttcc cgggtccggg tttgggcgtg cgtattttgg gcgaagtgaa
     4321 aaaagaatat gccgacctgc ttcgtcaggc agacgatatt ttcattcaag aattgcgcaa
     4381 tactaccgat gaaaacggta catcttggta cgacctgacc agccaggcat tcgccgtgtt
     4441 cctgcccgtc aaatctgtcg gcgtaatggg cgacggccgc acatacgatt acgtcattgc
     4501 cttgcgtgcc gtgattacca gcgactttat gaccgcgcat tgggcggaac tgccgtattc
     4561 cttgttgggc aaagtgtcca accgcatcat caacgaagtc aaaggcatca accgcgtggt
     4621 ttatgatgtg agcggcaaac cgcctgccac catcgagtgg gaataaacag caaacatggc
     4681 tgccccgtcc ggcgcagtcc ttcgattatc ggaaaaaagg aaaaaatatg agcacacaag
     4741 atttaaacgg caaaatcgct ttggtaacag gcgcatcgcg cggtatcggt gcagcaattg
     4801 ccgacacgct ggcggcagcc ggtgccaaag tcatcggtac ggcgaccagt gagagcggtg
     4861 cggcggcgat tagcgagcgg ttggcgcaat ggggcggcga aggccgcgta ttaaattccg
     4921 ccgaacctga aaccatcgaa agcctgattg ccgacatcga aaaagcgttc ggcaaactcg
     4981 atattctggt caacaacgcc ggcatcaccc gcgacaacct cctgatgcgc atgaaagaag
     5041 aagagtggga cgacatcatg caggtcaacc tcaaatccgt gttccgcgct tctaaagccg
     5101 ttttgcgcgg tatgatgaaa caacgttccg gccgcatcat caacatcaca tccgtcgtcg
     5161 gcgtgatggg caatgccggt caaaccaact atgccgcggc aaaagcaggc ttaatcggtt
     5221 tctccaaatc catggcgcgc gaagtcggca gccggggcat taccgtcaac tgcgtcgccc
     5281 ctggctttat cgataccgac atgacacgcg ccctgccgga agaaacccgc caaaccttta
     5341 ccgcccaaac cgccttgggc agattcggcg acgcgcaaga catcgccgat gcggttctgt
     5401 tcctcgcttc cgaccaagca aaatacatca ccggccaaac gctgcacgtc aacggcggta
     5461 tgctgatgcc ttaacagaca actttttcaa ccatgccgtc tgaagccctt tcagacggca
     5521 tttgcattct caggcaaaat gaacacacac cacaccccgc cctgcccatg cggctcaggc
     5581 acaagctgag acctttgcaa aattcctttc cctcccgaca gccgaaaccc caacacaggt
     5641 tttcagctgt tttcagctgt tttcgcccca aataccgcct aattctaccc aaataccccc
     5701 ttaatcct
//
LOCUS       DAWIVB010000138         5623 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWIVB010000138 DAWIVB010000000
VERSION     DAWIVB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.138"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(241..2373)
                     /locus_tag="WIX60_001642"
     CDS             complement(241..2373)
                     /locus_tag="WIX60_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ5305886.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(3155..3808)
                     /locus_tag="WIX60_001643"
     CDS             complement(3155..3808)
                     /locus_tag="WIX60_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ5305887.1"
                     /translation="MKTTHCKLKKKVQKELLRFFVLEVTARSAADILGIQPDSAILFY
                     RTIRMVISHYPALAADEVFEGSVELDESYFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNARSETLLPVIKRKIMPDSIVYTDSLSSYDKLHVSGFIHHRINHSKEFADRQ
                     NHINGIENFWNQAKRALRKYNEIDRKSFPLFLKECKFRFNFGTPSRQLKILRDWCGI"
     gene            4145..4579
                     /locus_tag="WIX60_001644"
     CDS             4145..4579
                     /locus_tag="WIX60_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305888.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQPYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4620..>5623
                     /locus_tag="WIX60_001645"
     CDS             4620..>5623
                     /locus_tag="WIX60_001645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ5305889.1"
                     /translation="MIGSGDTKQCKKFSADEFAKTIQSNFQSGIKYDGPTLATAGVSN
                     IIDEYLKDAYGGNGLKEAQRNLVAKEIADRIEAELHRQRANYQAQEAAKYSPAHGGVE
                     KSMYKHHADLQKTNTNIANRYSASANEYAAQAKSLGSLPKSVGYAALKNLGNALAALQ
                     MGEALYSNKANNIAKTGSSILGGMLAVAAAGAIASLAGVTLPFWASIAVGASIGLGIS
                     AAWDAFGVGEKLGLDPNVPTFYPFFGWLYDKTGGWEGGIPDWAKEWLGLNRDGKYHQY
                     DPLTLDLDGDGIETVATKGFAGALFDHRNQGIRTATGWVAADDGLLVRDLNGNGIIDN
                     G"
BASE COUNT         1662 a         1280 c         1250 g         1431 t
ORIGIN      
        1 tggaagaagg tgctcttgag aaatccccta aatgtcttgg tgggaattta ggggattttg
       61 gggaattttg caaaggtctc tagatgagtg aaaaagaagt gcaggctgcc taaaaagaca
      121 gaaaaagtct ttccggcagc ctgcactttg gtttcatttc agtcagtaaa cccagtaaac
      181 gacggtctaa aaacgcagaa cgttacgaaa aaagcagcct acacgcccat cccccgcctt
      241 ctacccgttc tgtaaatcat acagatagcg gtaatatccg ttcggcttcg ccagcaattc
      301 ctgctgtgtt cccgcttcca caatcctgcc tttatccatg gcaatgatcc ggtgtgccgt
      361 tttaacggtg gacagacggt gggcgataat cagcaccgtc cggttggcgc aaatggcctg
      421 catgttctgc ataatcgctc gttcactttc ataatccagc gcgctggtgg cttcgtcaaa
      481 aatcagaatg cgcggattgg tgattaacgc gcgggcaatc gcaatacgct gccgctgtcc
      541 gcccgacaag ccggcccctt gttcgcccac cacggtgccg tagccttccg gcagctccat
      601 aataaactcg tgtgcgcccg ccagtttggc tgcttcgata atgcgttcca gcggcatacc
      661 cgtatccgtc agcgcgatat tgtcgcgtat gctgcggttg agcagcacat tctcctgcaa
      721 gaccacgccg acctgccgcc gcagccaggc aggagcggcc aaagccaaat cgttgccgtc
      781 caccaacacc cgtccctgct ccggtacata cagacgctgc accaatttgg tgagtgtgga
      841 tttgcccgac cccgaacgtc ccacaatccc cagcacttcc cccgcccgaa tccgcaggtt
      901 caaatcctgc aaaatcagcc tgccgtccgc cttatagcgg aaatcgacat gttcgaacgt
      961 aatctccccc cggatatcgg gcaaagccaa atgcgaagac gcattctcgg tcggcgcatt
     1021 cagaatatcc cccaaacgcg ccaccgaaat ccccacctgc tggaaatcct gccacaactg
     1081 cgccaaacgg ataacaggcg ccgccacctg tcccgagagc atattaaacg caatcagctg
     1141 ccccaccgtc agcttgccct caattaccag ccgtgcgcca atccacaacg tcgccaccgt
     1201 caccagcttc tgaatcagct gcaccccctg ctggccgacc accgccaact tcgttacccg
     1261 aaatcccgaa gccacataag ccgccaactg attgtcccaa cgctgcgtca tctgcggctc
     1321 caccgccatc gcctttaccg tacccaccgc agtgatgctt tctactaaaa acgactggtt
     1381 gtctgcattg cgcgcgaact tatcgttcag acgcgtccgc agtatcggac tgataaatgc
     1441 cgaccaaaac gcataggcag gcaacgaagc caataccacc caagtcagag tggagctgta
     1501 ataccacatc accgccagaa agataaacga aaacgccaaa tccaacaccg aagtcagcgc
     1561 ctgaccggtc aagaaattgc gaatctgctc caattcccgc acccgagcca ccgtatcacc
     1621 cactcgtctg tgctcgaaat aggataaagg cagggaaagc agatgccgga acaaacgcgc
     1681 gcccaattcc acatcaatac gtgaagtcgt atgtgcaaac agatacgtcc gcaaaccgcc
     1741 caacacaatc tcaaacagcg acaccaccaa caaagccacc gacaccacat ccaaagtaga
     1801 gaatccccga tgtaccagca ccttgtccat caccacttgg aaaaacagag gcgtaatcag
     1861 cgcaaacagc tgcaacacca ccgacaccac caatacttca aaaaacaacc ggcggtattt
     1921 gattaccgcc ggaataaacc aggtaaagtc aaactttgcc aaactgccca ataccgaagc
     1981 gcgggaagca accaatatca gtttgcccga atatctgtta gaaaattcgg caaaagacaa
     2041 taccgcagac ttattcgtag ttaaatcctg tattagatat tgggcatgct cacccccacc
     2101 gtctgtttta gccaaaataa aatggttgcc gtcatcacac cataccaatg caggtaaggt
     2161 cgccatagcc aaacgtttga caggctggcg gactaccttt gccttcaatc ccaaagattt
     2221 ggcggctaac agccattgcg tttcatttaa atcgctctgt gcggaagtac aaaattcatg
     2281 ctgtatatcg gcaggattgg cggcaatgcc gtggtaatgg gcgaggatga tgagggcgga
     2341 aagggcgggg agcggtgcgg atacgataga cataaataaa atatagttag attggatgtg
     2401 gataacggct ggctggaaaa ggaatatatt aagtagaaag aaatatataa ataaaacagc
     2461 agaacgcatt gtaaggatat atatgggaat tgtaaagaga aagtatggaa aagttctcgt
     2521 ttcaggaagg taaaacggct taggaatcga gttagatgag gatgcctcgc acctctcgtg
     2581 cctcctgcat accgttaagg aacagggtta aggtgcaggc tgctccgaac tctgttgcgg
     2641 tcggataatg ttattttttg tgtttcaggc agcctgaaat atctgtatat ttttgtttta
     2701 aatagatttt aaagattgat aactgttctt gacgattttt caagaaagga gtaaagtgac
     2761 ttattatcaa tgacaagcaa cgcgcgaagt gacaaggaaa actatctact taaattctaa
     2821 ggaggcttcg aatatcataa accaatcaga aacatagaga taaaaattat gtacaaatat
     2881 aatcctctta tacaatttat tgcacagttg attatgtctt atggagcaag catagggtgg
     2941 gcacttgctg cccacgcgtt tcatatttca aggcagcctg aaactgtgtg ggcataatgc
     3001 ctaccctaca tcccaaaaaa acaagcgcag cctgcgtgtg tagggtgcga actttcggca
     3061 ggcagacacg cggttttata gtttcaagct gagttgggat gcttaagata aagtacaaaa
     3121 ccttaaaaaa taagggggct gtactagatt agccctaaat tccacaccaa tcccgcaaaa
     3181 tttttagctg tcgggacggt gtgccgaagt taaaccgaaa tttgcattct ttcaagaaca
     3241 gcgggaaaga tttacgatca atttcgttgt atttgcgcaa ggcgcgtttc gcctgattcc
     3301 aaaagttttc aatgccgtta atgtggttct gacggtctgc aaactcctta gaatggttga
     3361 tgcggtgatg gataaaaccg cttacgtgca gcttgtcata gctgctcaga ctatcggtat
     3421 aaacaatact gtccggcatg attttccttt tgatgacagg gagtaacgtt tcagacctgg
     3481 cattatctac aacaacggta tagacccgtc cgttgcgttt cagaatgccg aagacaacca
     3541 cttttcctgc cgcaccgcga ccgcgtctgc ctttacgccg tccgccgaaa tagctttcgt
     3601 ccaactcgac agagccctca aaaacctcat cggcagccaa ggccggataa tggctgatga
     3661 ccatacggat tgtgcggtag aacagaatag cggagtcggg ctggatgccc aaaatatcgg
     3721 cagctgaacg ggcggtaact tcaagtacaa aaaagcggag cagttctttc tgtacttttt
     3781 tctttaattt gcagtgtgtt gtcttcatat ttcgagggta acatatctgc taatctagta
     3841 cagcctcaaa caaaaaagag aaattttaat ttcgctaaat cacataaaga ttaatcaaga
     3901 gtatcattaa atgatatgag tgagcatcta taatgccaag aagagttgtt aagacataat
     3961 gattattgaa atagattgta aaatagatac ttagatagtc tgaaaaacgg atttgtgaaa
     4021 ctttttatta cgcgccatca tttgaaaatg aaacttaaaa aacacttatc ataataaata
     4081 ttttctttac gttgtttgct aataaactca gtgcaatatc atcgcaatat tttatggaaa
     4141 ttttatggat aacaaaaaag aatttattaa taatttaaca aataggtata tgtggatcta
     4201 tccattggtc ttaaatattc tatttctacc tttttaccag ccctaccaat ctttttttat
     4261 tgcgcttggt tgtttgtttg cactggttag aaaaatgcaa agcttagatt ttaaattaca
     4321 aaatcatatt gtattgttaa atataaaaag tgcttgggca gataaaaaag tatttttgat
     4381 taggatagta gtgtcatggt tggcggtaat ggaaatatgg atgtgtttta tttcggaatc
     4441 atcaacgtgg gtatgcggtg ctttttgttt aaatagtgaa atattggaaa aaatttttcg
     4501 tggctttggt tattctggta gtttatattt tttatttata ttgatgattg atctcaataa
     4561 gttaagagag agtatttgaa tttcattttt tgtttgactt aaactcaagg agaataacaa
     4621 tgattggtag tggtgatact aaacaatgca aaaaattttc tgcggatgag tttgcaaaaa
     4681 caatccaaag caactttcaa tcaggtatta aatatgatgg gccgactttg gcaacggcag
     4741 gcgtatctaa tataatagat gaatacttaa aggatgctta tggcggaaat gggttaaaag
     4801 aagcgcaacg caatcttgta gctaaagaga ttgcggatag aatagaggca gagttgcata
     4861 ggcagcgtgc taattatcaa gcacaagaag ctgcaaaata ttctcctgcg catggcggag
     4921 tggagaaaag tatgtacaag catcatgctg atcttcaaaa aacaaatact aatattgcta
     4981 atcgttattc tgcaagtgct aatgaatatg cggctcaagc aaaatcatta ggaagtttgc
     5041 caaaatcagt tggctatgct gcactaaaaa atttgggaaa tgctttggcg gcgttgcaaa
     5101 tgggagaagc tctatactca aataaagcta ataatatagc caaaacaggc tcttctattt
     5161 taggtggtat gttagcggtt gcagcagcag gtgctatagc tagtttagca ggagttacat
     5221 tgcctttttg ggcttctatt gcagttggtg catctattgg tttaggtatt agtgcagcat
     5281 gggatgcttt tggtgttggg gaaaaattag gattagaccc taatgtgcca actttttatc
     5341 cattcttcgg ttggctatac gataaaacag gcggctggga aggaggtatt cctgattggg
     5401 ctaaagaatg gttgggatta aaccgcgatg gcaaatacca ccaatacgac ccactaaccc
     5461 tagacttaga cggagacggc atagaaaccg ttgccaccaa aggctttgca ggtgcattgt
     5521 tcgaccaccg caatcaaggc atccgcaccg ccaccggttg ggttgctgcc gatgacggtc
     5581 tgcttgtgcg cgatttgaac ggcaacggca tcatcgacaa cgg
//
LOCUS       DAWIVB010000139         5561 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWIVB010000139 DAWIVB010000000
VERSION     DAWIVB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.139"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            11..577
                     /locus_tag="WIX60_001646"
     CDS             11..577
                     /locus_tag="WIX60_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ5305890.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRLILDGHENLKQYIPEPLDAAIFNFGWLP
                     GGDKNLTTDKETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLVFEKLRQK"
     gene            631..1005
                     /locus_tag="WIX60_001647"
     CDS             631..1005
                     /locus_tag="WIX60_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ5305891.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            1015..1509
                     /gene="folK"
                     /locus_tag="WIX60_001648"
     CDS             1015..1509
                     /gene="folK"
                     /locus_tag="WIX60_001648"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ5305892.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERNFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(1636..2115)
                     /locus_tag="WIX60_001649"
     CDS             complement(1636..2115)
                     /locus_tag="WIX60_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ5305893.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(2335..3072)
                     /gene="ubiE"
                     /locus_tag="WIX60_001650"
     CDS             complement(2335..3072)
                     /gene="ubiE"
                     /locus_tag="WIX60_001650"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ5305894.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(3115..3483)
                     /locus_tag="WIX60_001651"
     CDS             complement(3115..3483)
                     /locus_tag="WIX60_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ5305895.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            3603..5222
                     /gene="mnmC"
                     /locus_tag="WIX60_001652"
     CDS             3603..5222
                     /gene="mnmC"
                     /locus_tag="WIX60_001652"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ5305896.1"
                     /translation="MDNLVWDGIPDIRTLDQAIRKHAPPLNLIICLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQAMQCLQFDSINLIEHILPDVRFWLVPPSRTHHLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         1440 a         1752 c         1128 g         1241 t
ORIGIN      
        1 gcatttaaaa atgttattgc aaaacatcct tccattcgcc cattgccttt tgcggaaggc
       61 acttcccgaa ggcggcaatg cgctggacgg caccgccggc aacggacacg acaccctttt
      121 cctcgcacaa accgcaggca tccgggggaa agtgtgggca ttcgacatcc agccgcaagc
      181 cctgaacaac acccgatgcc gtctgcagga agcaggttac agcaatgtac ggctcatctt
      241 ggacggacat gaaaacctga agcaatatat tccggagccg ctggatgcag ccattttcaa
      301 tttcggctgg ctgcccggag gcgacaagaa tctgactacc gacaaggaaa ccagcattgc
      361 cgccctttct gccaccttat ccctactgaa agaaaacggt atgcttattg ccgtcctcta
      421 tccgggacac gaaaacggca aacaggaggc agaagcaatc gaacaatggg caaaaaacct
      481 gcctcaagaa cagtttgccg ttctgcgtta cggctttacc aaccggaaaa acagcccacc
      541 ctatcttttg gtatttgaaa aactgcgtca aaaataactg tttgcggtaa aataagccga
      601 ttctaaccct tattttgata cgagccgacc atgcagtatc tgattgtcaa atacagccac
      661 caaatcttcg ttaccatcac cattttggta ttcaacatcc gttttttcct actttggaaa
      721 aatccagaaa agcccttggc gggcttttgg aaggcactgc cccaccttaa cgacaccatg
      781 ctgctgttta cgggattgtg gctgatgaag attacccatt tctccccgtt caacgcacca
      841 tggctcggca caaaaatcct gcttctgctc gcctatatcg cattgggtat gatgatgatg
      901 cgcgcccgtc cgcgttcgac caagttctac accgtttacc tgctcgccat gtgttgcgtc
      961 gcctgcatcg tttaccttgc caaaaccaaa gtcctgcctt tctgaaacac cgttatgaac
     1021 aacagacatt ttgccgtcat cgccctgggc agtaatcttg aaaaccctgc ccaacaggta
     1081 cgcgccgcat tggacacgct gtcgtcccat cctgacatcc gtcttaaaca ggcttcctca
     1141 ctgtatatga ccgcacccgt cggttacgac aatcagcccg atttcatcaa tgccgtctgc
     1201 accgtttcca ccaccttgga cggcattgcc ctgcttgccg aactcaaccg tatcgaagcc
     1261 gatttcggac gcgaacgcag cttccgcaac gcgccgcgca cattggattt ggacattatc
     1321 gactttgacg gcatctccag cgacgacccc cgactcaccc tgccgcatcc gcgcgcgcac
     1381 gaacgcaatt tcgtcatacg ccctttggca gaaatcctcc ctgattttat tttgggaaaa
     1441 cacggaaagg ttgccgaatt gtcaaaacgg ctgggcaatc aaggcatccg tcttttaccg
     1501 gataagtaat tccgcacgcg gatgccgtct gaatgccggt ttcagacggc atttttcctt
     1561 tgccgccaac acgcgtgcaa aaaaatcgcc ccttgggaaa ggggcgcaaa aggaacacaa
     1621 accactacca aaactttaaa tctgcaacat tgcctgccgc atactgtatc cgacaggata
     1681 taaagccctc actaaatcgt ttcgagaaat ccaaacttct tcatcgccga cagaaaatct
     1741 gcctttctcc ggcaccagct ccaacaaaaa cggttgaacc gccgtatgca gcctgtcctt
     1801 acaccgcccc agctttctgg acaaggcgga cagggcgcgt ttgtaggcat tatccttgca
     1861 gtcaagttcc cgcgcactgc cgacccagct caaacgaagg ttgcggcttt catcctcaat
     1921 cagcaaaatg cctgttttga cttcccccac ctcgggctcg catgaaagcg caatataata
     1981 tttgccgtcg atgttgacat acgccgcttc atgcacctga cagttgcagc cggaagtggc
     2041 aagcagcgaa taaacagaat cccgatgcag tttcaaaata tcaagggaaa tctgtttgaa
     2101 aatactttcc ggcatttttc cctcctttcg tctgctgcgt tgtttactgg attgactgca
     2161 ttatacagac atctcccaat cttgcaaatg ataattgttc ctatttttta aaaaatttat
     2221 aaattattga tggatatgtg aattaatttt aagatttaaa atctctttct caaaaaaaca
     2281 caaaaatgcc gtctgaaacg tgttcagacg gcattggctg cacagccagt ccgtttaaaa
     2341 tttcacgccc ttatgcagcg cgacgatgcc cgcactcata ttgtgataat ccacgctgtc
     2401 gaagcccgca tccagcatca tctgtttcaa agtttcctga tcggggtgca tacggatgga
     2461 ttcggcaaga tactggtaac tctccgcatc tttcgcaatc agcctgccca tgaccggcag
     2521 cagcttgaac gaatagaaat catacgcgcc ttccaaaggt ttgtagattt tggaaaactc
     2581 caataccagc aatgtcccgc cgggtttcaa aacacggtac atctctttca gcgcggcatc
     2641 tttatgcgtc atattccgca aaccgaacgc cacggaaacc aagttgaaat aattgtcagg
     2701 gaaaggcagt ttttccgcat cggcaagcga tacgggcaaa atcatgcctt cgttcaacag
     2761 acggtcgcgc ccgacggtca gcatagagga attaatatcg gtcagccaaa cctcgccttc
     2821 cttgccgacc cgtttcgccc aaccgcgcga caaatcgccc gtaccgcccg caatgtccaa
     2881 cactttatcg ccttttttca ggtgcgccgt gttgatggtg aaatgcttcc acaccctgtg
     2941 cagccctgcc gacatcacat cgttcataat gtcgtagttt ttggcgacgg agtggaacac
     3001 ttccgccact tttccggctt tctcatcttc gttgaccgtg ctgaacccga aatgcgtttt
     3061 ttgtccgccc atattctaac cacctctctg ataccgccct acggcatccc aaacttaaca
     3121 atctgattgc ttttcaaaac gggaagcacc cgccgccgcc aatttgtcca aatattcctg
     3181 ccacatcgca tcgtatccgt atgccagtct gtgcagatac gcccaatcgt aaagaccgct
     3241 gtcgtgcccg tccgaaaaac tgattttcag cgcgtactgt ccgacaggct gcaaatccgt
     3301 gatttggaca tccgccttgc cggtctgcaa aacatcctgt cccacgccgt gtccgcgcac
     3361 ttccgcactc ggcgaataga cgcgtaaaaa ttcggcaggc agatttttag gttcgtcccc
     3421 ataagtcaaa accaacacgg tacggttttt caacaaacgg atttcaacag gaatattatt
     3481 caaaaaacac ctcggttcgg catacaatat ccggcaggcg caggcgtgcc ttcaaacggc
     3541 gcgtatttta acagctttat cccgataccg atattcaaac ccacacttta aacggcattg
     3601 atatggacaa tctcgtatgg gacggcattc ccgacatccg cacactcgac caagccatcc
     3661 gcaaacacgc acccccgctc aacctgatta tctgcctccc cgataatcag attcccgatt
     3721 ttcaaaccgc acaagatgct tcggacgcgg aatgccgtct gaagcaccgt ttggatcagg
     3781 caatgcagtg cctccagttc gacagcatca acctcatcga acacatcctg cccgatgtcc
     3841 gcttctggct ggttccccct tcacgcaccc accacctgca cgaacatttc caccacattt
     3901 cctggcagac cgaagccatc ccgcaaaccg aaagcaagcc cgacaaaccc tggtttgcac
     3961 ttccacaaac atccgaacgg caaaaaccgg aacacatcct cgttatcggc gcgggcatat
     4021 ccggcgcggc aaccgcccac gccttagcat cacacggcat ttccgttacc gtattggaag
     4081 cccgaaaagc cgcccaagcc gccagcggca accgccaagg gctgctctac gccaaaatct
     4141 cgccgcacga caccgaacag accgaacttt tgcttgccgg ctacggctac accaaacgcc
     4201 tgctcggaca catcctgccc gaatccgaaa cctggggcgg caacggcatc atccacctca
     4261 attacagccg caccgaacaa caacgcaatc acgaattggg tttgcaaaaa caccataacc
     4321 acctctaccg cagcatcaca tctgcagaag ccgaaaaaat cgccggtatc ccactgtccg
     4381 tcccatacga ccacccttca tgcggactct actggcaaca cggcgtatgg ctcaatccac
     4441 ccgcattcgt ccgcaccctc ctcaaccatc cgctcattgg actacacgaa gacacaccct
     4501 tgaccgacat ttcccacgac ggggaaaagt ggattgcaag cacgccaaac ggcacattta
     4561 ccgccacaca catcatctac tgcaccggtg cgaacagccc ctacctaccc gaaaccaacc
     4621 tcgccgccct gcctctcagg caaatacgcg gacaaaccgg cctcacaccg tccaccccgt
     4681 tttccgaaca actgcgttgc gccgtttcag gcgaaagcta catcagcccg tcgtggcacg
     4741 gactgcactg ctacggcgcg agttttattc ccaacagcag ccataccgga tggaacgaag
     4801 ccgaagaagc ctcaaaccgc caagcattgg cacaccttaa ccccgccctt tccgaatcat
     4861 tgtttgccgc caacccaaac ccccaaaaac accaagggca cgccgccata cgctgcgaca
     4921 gccccgacca ccttccccta gtcggcgcac tcggcgacat tgccgccatg cggcagacct
     4981 acaccaaact cgcgctggac aaaaactacc gcatcgacac cccatgccca tacctgccta
     5041 atgcctacgt caacaccgcg cacggcaccc gcggactcgc caccgccccc atctgcgccg
     5101 ccgccattgc agcccaaatc ctaggcttgc cccatccctt ttcacaacgc ctgcgccacg
     5161 ccctacaccc caaccgcacc atcatccgcg ccatcgtcag aaggaaggat ctaacccctt
     5221 aagtctttgc cgcatgccgt cccgaccaca gaaaatcata ttcaaataaa cataccaatc
     5281 ctgacggtaa aatatcgaaa acacccataa cgttttcttc ccaaaataag gagtaaggaa
     5341 tgcctaacaa attgcctttg ctgtccaaga tgaatgttcc gtaacattcg aagacttcct
     5401 taaccctgcc agccgtttcg gtttctgtac cccctgttag ataaggaagg aatgaaaaag
     5461 ccctgttgat tgtatgaagt aaagggcgtt aatttttgtt aatcttccct tctcaggaac
     5521 tcaatatata aggatgttaa tttttatttc ccttctcgag g
//
LOCUS       DAWIVB010000140         5551 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWIVB010000140 DAWIVB010000000
VERSION     DAWIVB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.140"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(43..810)
                     /locus_tag="WIX60_001653"
     CDS             complement(43..810)
                     /locus_tag="WIX60_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ5305897.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(898..1725)
                     /gene="mutM"
                     /locus_tag="WIX60_001654"
     CDS             complement(898..1725)
                     /gene="mutM"
                     /locus_tag="WIX60_001654"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ5305898.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1777..2442)
                     /locus_tag="WIX60_001655"
     CDS             complement(1777..2442)
                     /locus_tag="WIX60_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ5305899.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            2621..4597
                     /locus_tag="WIX60_001656"
     CDS             2621..4597
                     /locus_tag="WIX60_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ5305900.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWGELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHKISASPQHTAAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4665..5357
                     /locus_tag="WIX60_001657"
     CDS             4665..5357
                     /locus_tag="WIX60_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ5305901.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHSLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
BASE COUNT         1450 a         1785 c         1201 g         1115 t
ORIGIN      
        1 tccacaaaag cgaatcatac tgccacattc cgacaaccgg tttcagacag cgatatccgc
       61 atcgtcggcg acaacggcac ggatgctttc ttcgatttta tcttttaaag cataacggtc
      121 ttcgctttcc gccgcatccg ccacgcaaac gaaatcgact cttatcgtca attttttcat
      181 agacacgatg cgccacaggc aggtcggcaa accgacatcg gcatatgagg gacgagccgt
      241 cctttttccc gtttcgtcat aataacgcag cgcgaccgcc aaaacctttg cccccgcatc
      301 gatggcggat tggaacagcg cggctttgaa cggcaaaagc cccaatccgg aggaagtccg
      361 cgcttcgggg aaaaaactga cgttttgacc gcgttgcaag gtttcgcaga cggcgcggtt
      421 aatcggttcg atgtcgcgcc gcgaattgcg gttgatgaac accgttcccg cgttctgccc
      481 catcttgccc aataccggcc agcttttgat ttcctgcttg gcgataaagc tgctcggata
      541 aaccgcgctc atcgcgaaaa tatccagcca ggacacgtgg ttggcggcaa ccaagacacc
      601 gttcggatgt tcgggtgcgg gtctgcccac ctccaatccg atatccaaag ccgccaaaac
      661 ccccctgccc aactcgatta ccgcccgatt gcgcgactcg gggcaaccgc cgtcaatacc
      721 gcgcaggttt ttcccggttt tgaacagcca gaccgccaaa cggcacaagc ggcgcagacg
      781 tgtaaaaaat gaagctttat ttgaagacat gagcaattct ttcccgataa tcggtgtttg
      841 ttgttgaaac gttcggacgg cataccgata aaatgccgtc tgaaaccgtt ttcagtccta
      901 tttctgacag ttcgggcaat aaaacgtgcc gcgctgcccc aaagtttctt tcacaaccaa
      961 accgccgcac cgggggcacg gctgattgtg ccgcccgtac actgtatatt cctgttggaa
     1021 gtagccgctt ttgccgtcgc tgtccacaaa atccctcaag gtactgccgc ccgtttcaat
     1081 ggcgcgctgc aacaccgctt tgacggtttc aaccaaaagc gcgcactctt tctttttcag
     1141 gcggttggca ggacggtggg gcgaaatgcc cgctctgaac aggctctcgt tggcataaat
     1201 gttgcccaca ccgaccacga ccgcattgtc catcagggca agtttgaccg cgcgcttctg
     1261 cgccttcagc cttgcataca gataatccgc acaaaatgcc tccgacaaag gctccggccc
     1321 cagtttttcc aacagcggat gatgttcttc gattccctca taccaaagta tcgcgccgaa
     1381 ctttctcgga tcgcggtaac gcatgaccgt gccgtctgaa aacacaatat cgacgtgatc
     1441 gtgtctgtcc ggcctgccga tacgtccgtc cgacggcgta aaaatccgca agctgcccga
     1501 catccccaag tgaatcagca gcacgcccgt ttgaaagcgg ataagcaggt atttcgccct
     1561 cctgccgcag gacaacacct gccggccgga caaaatctcc cccaaatcgg gattaatctg
     1621 ccagcgcagc ttcaattggc gcaataccac ggcttccacc gttttccctt caatatgcgg
     1681 cgcgatgccg cgcaacgtcg tttccacttc cggcaattca ggcataaccc ctcccgacat
     1741 ttcttctgac agatgccgtc tgaaagacgg ctgtccctaa tccgcaaccc ttgccgcacc
     1801 cgccgcaagg gctttgccgc ccaaataccc gtcccacgcg ggcaggggcg ttacgcccgc
     1861 tgcccttttg accaaaacca aaccgtacac tgcggcacac tgcggccacc aacggtcgcc
     1921 cgccttttcc ataaaccgcc aaaagcgtat ttgcccgagc gacgaaaccg gcggcagata
     1981 caccataaat ttcccaaatt caatatcgaa accgacatcc gcaagccgtc ttttcaactc
     2041 gggcagcggc agacaaaacc gtttttccgg caggcgttcg ccgtcaaacc aacggctgaa
     2101 tccccagagc gaatacggat tgaaacccgt cagcatcaag cgtccgcacg gtttcaatat
     2161 ccggtgcgct tccgacagga tttgcgaagg aacaccgcct tcaagcgtat gcggaaaaag
     2221 cagcatatcc gcagaaacat ccgccaaagc catattctcc gccgacatcg acatatctcg
     2281 cggcacacag acaacatctt cagacaggct cagccacgga ccgcccacct gaaccgcaca
     2341 cattcccgaa aaacggtatg aatccagata ccgcccgaag aaatcctgtt ccaattttgc
     2401 aacataccgc cccatcgccg tatcttcaaa ccatgcatcc atattgcgtc cgtttcaaac
     2461 agattgcctg ccgatattct attccaaaca ggatttctgt caaaaaacac atcggccgcc
     2521 catttccgaa tccgcataaa gttcctgtaa aacttgacgc tttttcagtc aaacagtacc
     2581 atcggacgat aaaatatgtt tattccgccg aatataaatc atgtccaaac tcaaaaccat
     2641 cgctctgacc gcatcaggtc tgtccgtttg tccgggtttc ctatacgccc aaaacacctc
     2701 atcacaccaa atcggtttgg cgattatgcg cttaaactct tcaatactcg acctgccccc
     2761 gacaaaacaa tatttccaat ccggcagcct gtggggcgag ctgcgccaag gcttccggat
     2821 gggcgaagtc aatcccgaac tggtacgccg ccacgaaagc aaattcatcg caagccacag
     2881 ctatttcaac agggtcatca accggagtag accctatatg taccatatcg ccaacgaagt
     2941 caaaaaacgc aatatgcccg ccgaagccgc cctgcttccc ttcatcgaaa gcgcgttcgt
     3001 caccaaagcc aaatcacacg tcggcgcatc aggattatgg cagtttatgc ccgctaccgg
     3061 caggcattac ggcctggaaa aaacaccggt ttacgacggc aggcacgacg tttacgccgc
     3121 caccgatgcc gcactcaact atctgcaata cctctatgga ctgttcggcg actggccgct
     3181 tgcctttgcc gcctacaact ggggtgaagg caacgtcgga cgcgccatca accgcgcccg
     3241 cgcccaaggg ctcgaaccga cctacgaaaa cctgcgtatg cccaacgaaa cgcgcaacta
     3301 tgtccccaag ctgctcgccg tgcgcaacat tattgccact ccccaatctt tcggcatgaa
     3361 tatcagcgac atagacaaca aaccctattt tcaggcagtc gaaccggatc gtccgctcga
     3421 caacgaagcc atcgcccggc ttgccggcat cacgcaaagc gagctgctcg ccctaaaccc
     3481 cgcattcaac gtccccgcgt ttatccccaa aagcaaacgc aaactgctgc ttcctgtcgc
     3541 gtccgtacaa accttccaaa gcaactacct caacgccgca cccgacagcc tgttttcatg
     3601 ggaagtctat acgcctgccg ccaaaaccag cctgtccgac atctcgacgg caaccggcat
     3661 gagcattgcc gacatcaaac gcctcaacaa cctgaacggc aaccttgtca acgcaggacg
     3721 cagcatcctt gtcgccaaga acggcaaaac ccttcagacg gcatcggaat ccgtcgtttc
     3781 catcgacatc gacaatacgc ccgacaccta ccgttccaat atgccggcag gcacggtgaa
     3841 cgtcggcatt gcccgaatcc gacccgccgc cgcacagaca gcggacatta ccgtcgcacc
     3901 tttgccgcag aaaaccgtcc gtacggaacc cgatcccctt gtccgtattg ccgaacctgc
     3961 ccttgcgaca gccgcagcgc aacctcaaac cgaaaaacag accgccatgc cgtctgaaac
     4021 ccaaaccgca acactcgcgc agatcatccc ccaaaacgac atgcaggcgg cagacgaact
     4081 catgcagctt gttgcccgaa acaacctgcg ccgacaggct gaagaaacca tctccgccgt
     4141 catcggcacg cctgacacag ttgccgaaca caaaatttcc gcatctccgc aacataccgc
     4201 tgccgccgac ggcaaacgcc gggtacgttt ggaaacgcgc gtagccaaag ctgccgacgg
     4261 cgaagccgaa atctccccgc tccatgccag catccaccgc gttgtagaag gcgacaccct
     4321 gttcaacatt gccaaacgct acaacgtcag tgtagccgac ctgattgtcg ccaacaacat
     4381 caaaggcaac accatccaaa aaggacaggt actccgcctg gcgcaggcag cccctgccca
     4441 aacccgtatt gaaaaagtat cctacaccgc gcgcaaaggc gacaccttca aaagtatcgc
     4501 cgcgcgcttc aatatccata tcgacgacat ccgccggctc aatcccaacc tgaacaccat
     4561 caatccggga cagagggtca aactgattgg aagctgattc ggatacggca catgacagga
     4621 ctttctcagt cctgtctttt tcatcccaca tttcccatac catcatgaaa cttatcaaat
     4681 acctgcaata tcaaggcata ggaagccgca agcagtgcca atggctgatt gccggcggtt
     4741 atgttttcat caacggaacc tgcatggacg acaccgatgc agacatcgat tcctcatccg
     4801 tcgaaacgtt ggatattgac ggggaagcag taaccgtcgt tcccgaaccc tatttctaca
     4861 tcatgctcaa caagcctgaa gattacgaaa cttcgcacaa acccaagcac taccgcagcg
     4921 tattcagcct gttccccgac aatatgcgga acatcgatat gcaggcggtc ggcaggctgg
     4981 atgcagatac gaccggcgta ttgctgatta ccaacgacgg caaactgaac cacagcctga
     5041 cttcgccgag cagaaaaatt cccaagctgt acgaagtaac gctcaaacac cccacaggag
     5101 aaacgctctg cgaaaccttg aaaaacggcg tgctgctcca cgacgaaaac gaaaccgttt
     5161 gtgccgccga tgccgttttg aaaaacccga ccaccctgct gctgaccatt accgaaggaa
     5221 aataccacca agtcaaacgc atgatcgccg ccgccggcaa ccgcgtgcaa caccttcatc
     5281 gccggcgatt cgcacatctg gaaacagaaa acctcaaacc cggggaatgg aaatttatcg
     5341 aatgtccaaa attctgaaat aacatccaag aattccattt atattcatcc acatactcat
     5401 taaatatatg gtttaaccca atttaacgca aaaaatcaat ttacgatata atccatttgt
     5461 ctgagtaacg cctgttcaag caggcttatg agtaagacgt tttccccgta atgtgttttg
     5521 ccatctatac ttctcccctt gtatagatgg t
//
LOCUS       DAWIVB010000141         5545 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWIVB010000141 DAWIVB010000000
VERSION     DAWIVB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.141"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            52..1455
                     /locus_tag="WIX60_001658"
     CDS             52..1455
                     /locus_tag="WIX60_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ5305902.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALEWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKSAAQGLPEAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVEPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            1577..2293
                     /gene="trmB"
                     /locus_tag="WIX60_001659"
     CDS             1577..2293
                     /gene="trmB"
                     /locus_tag="WIX60_001659"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ5305903.1"
                     /translation="MTDTAENQTQNNWQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(2921..3283)
                     /locus_tag="WIX60_001660"
     CDS             complement(2921..3283)
                     /locus_tag="WIX60_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305904.1"
                     /translation="MSQLDFNLELFIKNSNFDDYKIFDIVKEAIETQDEIGSILRTHL
                     FFERILEAWIYAKCNKEDFFNDTQIGFRTKLKIARNLGLFSEVYDIGERLNKIRNTIA
                     HHVLREVNQENLTLYMKV"
     gene            complement(3396..5423)
                     /gene="uvrB"
                     /locus_tag="WIX60_001661"
     CDS             complement(3396..5423)
                     /gene="uvrB"
                     /locus_tag="WIX60_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ5305905.1"
                     /translation="MEVIQYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRNDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTIFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIEK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEQGIVPQQIKKQVKDIIDGVYHEEDGSKGRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRDIKENLLFGAE"
BASE COUNT         1397 a         1412 c         1380 g         1356 t
ORIGIN      
        1 ctaacttcat aacgaataac gatgattcga caaaacggaa aacgatctga catgaacaat
       61 cccgacttac cctatcggca ggccttagaa tgcctgtctc aaaaacaata taactttacc
      121 gaagtccgcc gactgctgac agaagcgttc tcggcaggtc atcccgccgc cgcattcgag
      181 ttggcaaaac acctgatgga cgcggacagc ccctaccaag accgcgaaca aggtatggaa
      241 atgctccgca tcgccgctga acagggacat ccctacgcgc gttacaatct ggcatatatc
      301 caagaattgg aaggcgcacc cccggaaacc ctgataccgc tttacagacc gttggcagaa
      361 gaaggactgc ccgaagcgca agtccgcctg atgtaccttc tgtacgcgtc ccgacatttt
      421 gaagaagcct tggaatgggc aaaaacaagc gcaaaaaaca acaaccccca cgggcaatac
      481 ctgcttgccc aatactgccg gtacggcaca ccgccggatt ttgaaacggc gcacctgctc
      541 taccgaaaat cggcggcaca aggcttgccg gaggcacatt ggcagctcgg gctgcaatat
      601 cgtttcgggc aagggacgaa agtcgacacg gcacaggccg tcaatcattt gcgcgccgcc
      661 gcacaacaag gatacattcc tgcctacacc ccacttgccg agctcatcct acctacggct
      721 cctgatgaag ccgttcactg gtttcaacag gccgcacagg aaaatgaccc cgatgcccat
      781 gccgcacttg ccgacatcta cctgcaaggc aagcatctgg aaagaaacca caaacttgcc
      841 ctgcatcatg ccgaagcagc cgccgccgaa cgccatcccg aaggtttgcg gatactgggc
      901 gacatctgcc gctacggttt gggcatagcc cccgatacgg aaaaagcccg gcattattat
      961 cggcaggcag ccgaagccgg cagcctttcc gcctatcaga aactcatatc cgacagcgcg
     1021 ttaaaccatc ctgaccaata cggcggcatt aaagattccg ccatcaggcg gcaaagggca
     1081 gaacggcttt atcaaaaagc ccaagccctg cattacggat tacaatgcgc gcccgaatac
     1141 gcagccgcgc tcaaactcta cacagaagcc gcagaactcg gacacagcaa agcccaaacc
     1201 aatctgggca gcatgtatta cttcggacag ggtatgaccg ccgactacaa tgaagcacgc
     1261 aaatggtttg aaaaagccgc cgcgaaaaaa gacagtatgg cgttctacaa cctcgcctgc
     1321 atccattaca gcggacacgg cgtcgagccg gacaaagaaa aagcctgccg ctacctgcaa
     1381 gaagccataa acaacggata cgggcaaaaa agcgtcctgc aagaactgct gcaacaatgg
     1441 caaaatgccg tctgaacagc gttacaccta ccctgccgaa acgaaacagg tataatcgcc
     1501 cctttccctt cccgccgtcc gaacaggcat ttcacattca gacggcatcc tgattgcaca
     1561 agcgtacgaa agcattatga cagacaccgc cgagaaccaa acacaaaaca actggcaagc
     1621 cggacacccc cgcagcatcc gcagcttcgt cctccgccaa agccatatga ccgccgcgca
     1681 gcaacgcgcc atcgatacct tatgggacag cttcggcatc gactaccaag caacaccggc
     1741 cgatcttgat gcccgtttcg gaagcagccg acccaaaatc ctcgaaatag gcttcggtat
     1801 ggggacggca accgcagaaa tcgcccgccg cctgcccgaa accgacttcc tcgccatcga
     1861 cgtacacggt cccggcgtag gcaacctgct caaactcata gacgaaaacc atttagaaaa
     1921 catccgcgtg atgcggcacg atgccgtaga agttgtcgaa aatatgctgc aagacggctc
     1981 gctcgacggc atccacatat tcttccccga cccgtggcac aaaaaacgcc accacaaacg
     2041 ccgtctgata caagccccct tcatcgccaa actactgccc aaactcaaaa ccggcggcta
     2101 tatccacctg gcgacagact gggaagaata tgcacagcag atgcttgaag tcctcagtag
     2161 cttcgacagc ctgcaaaata cggcggcaga ctacgccccc accccggact accgccccga
     2221 aaccaaattc gaagcgcgcg gcaaacgcct cggacacggc gtttgggact tggtattcaa
     2281 acggatcgga taacaaacca ctgtttgaaa atgccgtctg aaacatgttt gcttacagac
     2341 ggcatttttt caagataaag cagcaagtga tgtttcgata taaagtttaa aacaatagtt
     2401 tgaacggcaa aacgcgtgtg taccgcacgc atccttatag gttttatgca catcggtttt
     2461 aaagtttgtg ccgcccgcag gtagtatgtg atagctacgc acgcggttgg tgtgatgtag
     2521 gctacggctt gctggttaca accgtaaaaa agtaagtgcc gccattgcgg taaaaacgaa
     2581 gggatttcat agtgttatgc tcgtaatgat tttgtagatt ggattctcga atccgacctt
     2641 ttgggcattg ctgcaatgga ttgcaacgac gggaatgttg aaggttttgt cggatacaag
     2701 tatccgacct acgcttgttg ctatatatct tttgatttaa tgactaaata tgacaaagtt
     2761 aaagtgcaga ttacagcaag catgatatgc ttctttaggc ttttatcatt ccatgatata
     2821 gatatttctt ccttttcatt ttctttataa aattttaaac ctatatcacc atttttccat
     2881 tcctagtggt ttactatgat tttattttta aaagaatctc ttaaactttc atgtaaagag
     2941 ttaaattttc ttgatttact tcccttagta catggtgagc aattgtattt ctaattttat
     3001 ttaatctctc ccctatatca tatacttcgc taaataagcc aagattacgc gcaattttta
     3061 gttttgtgcg aaatccaatt tgtgtatcat tgaaaaaatc ttctttatta cattttgcat
     3121 atatccatgc ctctaaaatt ctttcaaaaa ataaatgtgt tcgtaagatt gaacctattt
     3181 catcctgtgt ttcaatagct tctttcacga tatcaaaaat cttataatca tcaaaatttg
     3241 aatttttaat aaataattct aaattaaaat ctaattgaga cataataagt gcccatttca
     3301 aaaataaatc tatattctag ttaatataat agttattcta atatctaaat taaataataa
     3361 actactattt ttatatccac gacaaagtct aagtctcact ccgccccaaa caacaaattc
     3421 tctttaatat ccctaatcct atcccgcaac acagccgcct cttcaaactg caaatcccta
     3481 gccgcctgct gcatggcttt ttcgagtttg gcgatttctt taatcgcatc ttcttcgttg
     3541 tgaatctcgc ccactttaac cttgttttta cctttcagac ggcctttact gccgtcttct
     3601 tcgtggtaca cgccgtcgat gatgtctttg acctgttttt taatctgctg cggcacgatg
     3661 ccctgttctt cgttgaattt aatctgtttt tcacggcggc gttcggtttc gtcgatagcg
     3721 gctttcatgg agtcggtaat tttgtcggcg tacaggatgg cgacgccgtt cacgttgcgc
     3781 gcggcgcggc ctatggtttg aatcaggctg cggtgggagc gcaggaagcc ttctttatct
     3841 gcgtcgagga tggcgacgag agagacttcg gggatgtcga ggccttcgcg taagaggttg
     3901 atgccgacga gtacgtcaaa caggccgagc cgtaaatctc taatgatttc aacgcgctcg
     3961 acggtgtcga tgtcgctgtg caggtagcgc actttgatac cgagttcgct gtaatagtcg
     4021 gtgagttgct ccgccatgcg tttggtgagg gtagtaacga gtacgcgttc gcctttttca
     4081 atgcggtcgt tgatttcgct cattaagtcg tcgacttggg tggcaacggg gcggatgatg
     4141 atttggggat caaccagccc tgtggggcgg acgacttgtt cgaccacttg tccggcgtgt
     4201 tcttcttcgt atttggcggg ggtagcggaa acgaagatgg tttgcggcat gactttttca
     4261 aattcgtgga atttgagcgg gcggttgtcg cgggctgaag gcaggcggaa gccgtagtcg
     4321 acgaggtttt gcttgcgcga tgcgtcgcct ttgtacatgc cgccgatttg ggttacggta
     4381 acgtggcttt cgtcgatgaa catgatggcg ttgtcgggca ggtagtccat cagcgtaggc
     4441 ggcggttcgc cttctttttt gccggaaaag tggcgggagt agttttcgat tcctttgcag
     4501 aagcccattt cgtagagcat ttcgaggtcg aaacgggtgc gctgttcgat gcgttgttgt
     4561 tcgacggggc gttgttcgcg ggcgaaaaat tcgatgcgtt cgcgtaattc ttctttgatg
     4621 gactcgcagg cgcgcaagac ggtgtcgcgc ggggtaacgt agtggctgga cgggaagacg
     4681 gtgtagcggc cgacgcgctg gataaggctg cctgaaagcg ggtcgaacat atcgaggcgg
     4741 tcgatttcgt catcaaacag gctgatgcgt aaggcgtttt cggagctttc ggcggggtac
     4801 acgtcaatca cgtcgccgcg cacgcggaag ctgccgcgtt tgaagtccaa atcgccgcgt
     4861 tcgtattgca tggaaacgag cgtggcgatg atgtcgcgct gctcgatggt atcgccttct
     4921 ttgacggaca acaccatttg ttgatactcg gtcgggtcgc cgataccgta aatggcggac
     4981 acggtggcga cgataatcac gtcgttgcgc gtcattaggt ttttggtggc ggaaaggcgc
     5041 atctgctcga tgtgttcgtt gatcgcgctg tctttttcga tgaacaaatc gcggctgggc
     5101 acataggctt cgggctggta atagtcgtag taggagacga aatattccac tgcgttttcg
     5161 gggaaaaatt cgcgcatttc ggcgtaaagc tgggcggcaa gggttttgtt gtgcgccatg
     5221 atgatggcgg ggcggccgct ttgggcgatg acgttcgcca tggtgtaggt tttgcccgaa
     5281 ccggttacgc cgagcagggt ttgataggca aggccgtctg aaagcccttc gagcaggcct
     5341 gcaatggcgg tgggctggtc gcctgcgggc gggaagggtt ggtggagttt gaagggggaa
     5401 tttgggtatt ggataacttc cataatcttg cctgtgatgc gtttgcggac aaagcgtgca
     5461 gtagggatgg gtcggaaacg tctttcagac ggcataaggc ggtgaaatcc tgaatgtatg
     5521 ccgtctgaaa cccaatcgct accca
//
LOCUS       DAWIVB010000142         5511 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWIVB010000142 DAWIVB010000000
VERSION     DAWIVB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.142"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..260)
                     /locus_tag="WIX60_001662"
     CDS             complement(<1..260)
                     /locus_tag="WIX60_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ5305906.1"
                     /translation="MLCPEKMSVMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQEL"
     gene            complement(318..431)
                     /gene="rrf"
                     /locus_tag="WIX60_001663"
     rRNA            complement(318..431)
                     /gene="rrf"
                     /locus_tag="WIX60_001663"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(528..3419)
                     /locus_tag="WIX60_001664"
     rRNA            complement(528..3419)
                     /locus_tag="WIX60_001664"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3825..3900)
                     /locus_tag="WIX60_001665"
     tRNA            complement(3825..3900)
                     /locus_tag="WIX60_001665"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3865..3867),aa:Ala,seq:tgc)
     gene            complement(3906..3982)
                     /locus_tag="WIX60_001666"
     tRNA            complement(3906..3982)
                     /locus_tag="WIX60_001666"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3946..3948),aa:Ile,seq:gat)
     gene            complement(4084..>5511)
                     /locus_tag="WIX60_001667"
     rRNA            complement(4084..>5511)
                     /locus_tag="WIX60_001667"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1194 a         1611 c         1189 g         1517 t
ORIGIN      
        1 tccaactcct gctgcgaagc cgcattgatg tttaccgccg caagggagaa ggcgcaggag
       61 aacagcatac agaacagcac gaacattttc ttcatggttt ttcctttaag ggttgcaaac
      121 aataaaccgc atcttgcgac gataaaacga gtcattctaa aatgaatatc ccaaagtttc
      181 aagccgttcc tccgcaaacc cgaccggaca ccgtacggat gccgtcccgc catcaccgac
      241 attttttccg ggcaaagcaa acattttttc cgggcaaagc aaaaaccccc gaataatcgg
      301 gggttttctg aatgggtgtt tggcagtgac ctactttcgc atggaagaac cacactatca
      361 tcggcgctga gtcgtttcac ggtcctgttc gggatgggaa ggcgtgggac caactcgcta
      421 tggccgccaa acttaaactg ttacaaatcg gtaaagcctt aatcaatata ttcggtaatg
      481 actgaatcag tcagtaagct tttatctctt gaagttcttc aaatgataga gtcaagcctc
      541 acgagcaatt agtatgggtt agcttcacgc gttaccgcgc ttccacaccc cacctatcaa
      601 cgtcctggtc tcgaacgact ctttagtgcg gttaaaccgc aagggaagtc tcatcttcag
      661 gcgagtttcg cgcttagatg ctttcagcgc ttatctcttc cgaacttagc tacccggcta
      721 tgcaactggc gttacaaccg gtacaccaga ggttcgtcca ctccggtcct ctcgtactag
      781 gagcagcccc cgtcaaactt ccaacgccca ctgcagatag ggaccaaact gtctcacgac
      841 gttttaaacc cagctcacgt accactttaa atggcgaaca gccataccct tgggaccgac
      901 tacagcccca ggatgtgatg agccgacatc gaggtgccaa actccgccgt cgatatgaac
      961 tcttgggcgg aatcagcctg ttatccccgg agtacctttt atccgttgag cgatggccct
     1021 tccatacaga accaccggat cactatgtcc tgctttcgca cctgctcgac ttgtcggtct
     1081 cgcagttaag ctaccttttg ccattgcact atcagtccga tttccgaccg gacctaggta
     1141 accttcgaac tcctccgtta cgctttggga ggagaccgcc ccagtcaaac tgcctaccat
     1201 gcacggtccc cgacccggat gacgggtctg ggttagaacc tcaaagacac cagggtggta
     1261 tttcaaggac ggctccacag agactggcgt ctctgcttct aagcctccca cctatcctac
     1321 acaagtgact tcaaagtcca atgcaaagct acagtaaagg ttcacggggt ctttccgtct
     1381 agcagcgggt agattgcatc ttcacaacca cttcaacttc gctgagtctc aggaggagac
     1441 agtgtggcca tcgttacgcc attcgtgcgg gtcggaactt acccgacaag gaatttcgct
     1501 accttaggac cgttatagtt acggccgccg tttactgggg cttcgatccg atgctctcac
     1561 atcttcaatt aaccttccag caccgggcag gcgtcacacc ctatacgtcc actttcgtgt
     1621 tagcagagtg ctgtgttttt aataaacagt cgcagccacc tattctctgc gaccctccgg
     1681 ggcttacgga gcaagtcctt aaccttagag ggcatacctt ctcccgaagt tacggtatca
     1741 atttgccgag ttccttctcc tgagttctct caagcgcctt agaattctca tcctgcccac
     1801 ctgtgtcggt ttgcggtacg gttcgattca aactgaagct tagtggcttt tcctggaagc
     1861 gtggtatcgg ttgcttcgtg tccgtagaca ctcgtcgtca cttctcggtg ttaagaagac
     1921 ccggatttgc ctaagtcttc cacctaccgg cttaaacaag ctattccaac agcttgccaa
     1981 cctaaccttc tccgtcccca catcgcattt gaatcaagta caggaatatt aacctgtttc
     2041 ccatcgacta cgcatttctg cctcgcctta ggggccgact caccctacgc cgatgaacgt
     2101 tgcgcaggaa accttgggct ttcggcgagc gggcttttca cccgctttat cgctactcat
     2161 gtcaacattc gcacttctga tacctccagc acactttaca atgcaccttc atcagcctac
     2221 agaacgctcc cctaccatgc cggtaaaccg gcatccgcag cttcggttat agatttgagc
     2281 cccgttacat cttccgcgca ggacgactcg accagtgagc tattacgctt tctttaaatg
     2341 atggctgctt ctaagccaac atcctggctg tctgggcctt cccacttcgt ttaccactta
     2401 atctatcatt tgggacctta gctggcggtc tgggttgttt ccctcttgac aacggacgtt
     2461 agcacccgct gtctgtctcc cgaggaacca cttgatggta ttcttagttt gccatgggtt
     2521 ggtaagttgc aataaccccc tagccataac agtgctttac ccccatcagt gtcttgctcg
     2581 aggcactacc taaatagttt tcggggagaa ccagctatct ccgagtttgt ttagcctttc
     2641 acccctatcc acagctcatc cccgcatttt gcaacatgcg tgggttcggt cctccagtac
     2701 ctgttacggc accttcaacc tggccatgga tagatcactc ggtttcgggt ctacacccag
     2761 caactcatcg ccctattaag actcggtttc cctacgcctc ccctattcgg ttaagctcgc
     2821 tactgaatgt aagtcgttga cccattatac aaaaggtacg cagtcacacc actagggcgc
     2881 tcccactgtt tgtatgcatc aggtttcagg ttctgtttca ctcccctccc ggggttcttt
     2941 tcgcctttcc ctcacggtac tggttcacta tcggtcgatg atgagtattt agccttggag
     3001 gatggtcccc ccatattcag acaggatttc acgtgccccg ccctactttt cgtacgctta
     3061 gtaccgctgt tgagatttcg aatacgggac tgtcacccac tatggtcaag cttcccagct
     3121 tgttcttcta tctcgacagt tattacgtac aggctcctcc gcgttcgctc gccactactt
     3181 gcggaatctc ggttgatttc ttttcctccg ggtacttaga tggttcagtt ctccgggttc
     3241 gcttctctaa gtctatgtat tcaacttagg atactgcaca gaatgcagtg ggtttcccca
     3301 ttcggacatc gcgggatcat tgctttattg ccagctcccc cgcgcttttc gcaggcttac
     3361 acgtccttcg tcgcctatca tcgccaaggc atccacctga tgcacttatt cacttgactc
     3421 tatcatttca agaacttctt tgactttgcc taacattccg ttgactagaa catcagactt
     3481 gaatttccta ctttgataaa gcttactgct ttgttgtgtc ttaatcctgc cttttgtgtt
     3541 tcaggattaa gtcgatacaa tcatcaccca aatactgtgt ttgttttctt ttctcttgcg
     3601 agagattttt atcctttgca aagaataaaa aatcaaaaca aacgctttgt ctttgtttgt
     3661 tgatttcggc tttccaattt gttaaagatc gatgcgttcg atattgctat ctactgtgca
     3721 aatcaaaacg agctgattat tatatcagca ttttgttctt ggtcaagtgt gacgtcgccc
     3781 tgaatggatt ctgttccatt cttccgtttt gatttgtaca gtattggtgg aggcaaacgg
     3841 gatcgaaccg atgaccccct gcttgcaaag caggtgctct accaactgag ctatgccccc
     3901 gttcttggtg ggtctgggag gacttgaacc tccgacccca cgcttatcaa gcgtgtgctc
     3961 taaccagctg agctacaaac ccggattctc ttcttaagcg aatcttgcct tcactcaagc
     4021 ttcttccgca tctttttcag tttaccgata agtgtgaatg cctaaagcct cttctttctc
     4081 tagaaaggag gtgatccagc cgcaggttcc cctacggcta ccttgttacg acttcacccc
     4141 agtcatgaag cataccgtgg taagcggact ccttgtggtt atcctaccta cttctggtat
     4201 cccccactcc catggtgtga cgggcggtgt gtacaagacc cgggaacgta ttcaccgcag
     4261 tatgctgacc tgcgattact agcgattccg acttcatgca ctcgagttgc agagtgcaat
     4321 ccggactacg atcggttttg tgagattggc tccgcctcgc ggcttggcta ccctctgtac
     4381 cgaccattgt atgacgtgtg aagccctggt cataagggcc atgaggactt gacgtcatcc
     4441 ccaccttcct ccggcttgtc accggcagtc tcattagagt gcccaactga atgatggcaa
     4501 ctaatgacaa gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga
     4561 cgacagccat gcagcacctg tgttacggct cccgaaggca ctcctccgtc tccggaggat
     4621 tccgtacatg tcaagaccag gtaaggttct tcgcgttgca tcgaattaat ccacatcatc
     4681 caccgcttgt gcgggtcccc gtcaattcct ttgagtttta atcttgcgac cgtactcccc
     4741 aggcggtcaa tttcacgcgt tagctacgct accaagcaat caggttgccc aacagctaat
     4801 tgacatcgtt tagggcgtgg actaccaggg tatctaatcc tgtttgctac ccacgctttc
     4861 gggcatgaac gtcagtgttg tcccaggagg ctgccttcgc catcggtatt cctccacatc
     4921 tctacgcatt tcactgctac acgtggaatt ctacctccct ctgacacact cgagtcaccc
     4981 agttcagaac gcagttcccg ggttgagccc ggggatttca catcctgctt aagtaaccgt
     5041 ctgcgcccgc tttacgccca gtaattccga ttaacgctcg caccctacgt attaccgcgg
     5101 ctgctggcac gtagttagcc ggtgcttatt cttcaggtac cgtcatcagc cgctgatatt
     5161 agcaacagcc ttttcttccc tgacaaaagt cctttacaac ccgaaggcct tcttcagaca
     5221 cgcggcatgg ctggatcagg cttgcgccca ttgtccaaaa ttccccactg ctgcctcccg
     5281 taggagtctg ggccgtgtct cagtcccagt gtggcggatc atcctctcag acccgctact
     5341 gatcgtcgcc ttggtaggcc tttaccccac caactagcta atcagatatc ggccgctcga
     5401 atagcgcaag gcccgaaggt cccctgcttt ctctctcaag acgtatgcgg tattagctga
     5461 tctttcgatc agttatcccc cactactcgg tacgttccga tatgttactc a
//
LOCUS       DAWIVB010000143         5481 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWIVB010000143 DAWIVB010000000
VERSION     DAWIVB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.143"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            65..490
                     /locus_tag="WIX60_001668"
                     /pseudo
     CDS             65..490
                     /locus_tag="WIX60_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            496..1725
                     /locus_tag="WIX60_001669"
                     /pseudo
     CDS             496..1725
                     /locus_tag="WIX60_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            1783..2403
                     /locus_tag="WIX60_001670"
     CDS             1783..2403
                     /locus_tag="WIX60_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5305907.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLIGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            2414..2545
                     /locus_tag="WIX60_001671"
                     /pseudo
     CDS             2414..2545
                     /locus_tag="WIX60_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2542..3036
                     /locus_tag="WIX60_001672"
     CDS             2542..3036
                     /locus_tag="WIX60_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ5305908.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3124..4785)
                     /locus_tag="WIX60_001673"
     CDS             complement(3124..4785)
                     /locus_tag="WIX60_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ5305909.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4915..5457)
                     /locus_tag="WIX60_001674"
     CDS             complement(4915..5457)
                     /locus_tag="WIX60_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ5305910.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVVTLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
BASE COUNT         1307 a         1486 c         1398 g         1290 t
ORIGIN      
        1 tatcccgtat gcggattggt tttaagattt gtaaatttga tttgcatcaa aaaatcgccg
       61 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
      121 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacggcgt tttttgccgc
      181 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
      241 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacggcaagc cggaaggtaa
      301 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
      361 cgatgcggtc aatacgcgcg gttattcgag caaaccgatt gatacgctga tggtgttggc
      421 aaacgacggc acgatagccg gggcgaaact ggtcgaccat cacgaaccga ttatgctgat
      481 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc tcaaactggc ttccggcgta
      541 tataaaacca aacttcacat tgacaaaccg attacgattg aagggcctgc cgaccgttcc
      601 gcaaccatcg aaggcgacag gagcgggcgt accatagccg tacacgcgcc ggacgtaacg
      661 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc ccgcaatgga tgccggtatt
      721 tatctcgaag aaactgcccc gcgcgccctg attgaacaca acaatatttt ggataattcg
      781 gtcggcgtat atctgcatgg ttctgccgat gcgatggtgc gcgagaataa aatcgtcggc
      841 gacgcgactt tgcgcgtgaa cgagcgcggc aacggcgtta ccgtttggaa cgcacccggt
      901 gcgcaggtcg tcggcaacga catttccaaa gggcgggacg gcattttttc caataccagc
      961 acgcacaaca cctacaaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
     1021 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat gggctatgtg
     1081 ctgatgtttt ccgagcggct caaagtattc gacaatatcg ccgtcggcag ccgcgatcag
     1141 ggcattatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaggca
     1201 ggcaagtgcg tatttgccta taatgccaac tacgataaac ttttcgccaa tcattttgaa
     1261 aactgtcaaa tcggcataca ctttaccgcc gccatcgaag gcacgtcctt gcatgacaat
     1321 tcctttatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgattggagc
     1381 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
     1441 ttcggagaca gcgcgtaccg ccccaacggc atcatcgacc aaatcatctg gcgcgcgccc
     1501 gtatcgcgcc ttttgatgaa cagtcccgca atcagcatcg tcaaatgggc gcaggcgcag
     1561 tttcccgccg ttctgcctgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
     1621 cccaaaattc aaacccgtta tcaggcgatg aaggacgagc tactcaaaga agtcgaaacg
     1681 cggcagtcgg aatggggcag ggcggaaaac ggttctttga actagtctgc ttcagacggc
     1741 atccggattc aaatgccgtc tgaaaacaca aaaggaacaa ccatgaccac acatcatgtc
     1801 gaattgagga aggtaaccaa acggttcggg gcgcaaaaag ccgtcaacca agtcgatttg
     1861 gttttgaagg caggagaaag cgtcgggctt gccggacaca acggcgcggg caagtccacc
     1921 attatgaagc tgatactcgg gctgattacc ccgaccgaag gcgaagtgat gcttttgggc
     1981 gaacgtaccg gtagcaaagc gggggcgcgg cttcgcagcc aaatcggcta cctgcccgaa
     2041 accgttgcgc tgcacccttc gctgatcggc atcgaaacgc tggattttta tgccaaactt
     2101 aaaaaacagc cgctcacgca gaaccggggg ctgcttgagc gcgtcggcat ttcacaggcg
     2161 gcacaccgcc gcgtcggcac ttattctaaa gggatgcgcc aacgccttgc cttggcacaa
     2221 gccctgctgg gcgagcccaa agtcctgctg tttgacgaac cgacaaccgg tcttgaccct
     2281 gcatcacgac aaatgtttta cgaagtcgtg cgcgaactca acgggcgcgg cgcgaccgta
     2341 ttgctcagca cccacgccct tgccgagttg gacgggcacg ccgaccgcat tatcgtggat
     2401 taaatttaat ccactatatg cgggtatggc gggtttgagc ggacaaatca gcctgaccgt
     2461 ccccgttttg ctgaccgctc aggttttatg ggttatcatt ccgcttgttt tggcagccgg
     2521 aatttttaga aagcgacaaa tatgaaaaaa accctgttgg caattgttgc cgtttccgcc
     2581 ttaagtgcct gccggcaggc ggaagaggga ccgccgcctt taccccggca gattagcgac
     2641 cgttcggtcg gacactattg cagtatgaac ctgaccgaac acaacggccc caaagcccag
     2701 attttcttga acggcaaacc cgatcagccc gtttggttct ccaccatcaa gcagatgttc
     2761 ggctatacca agctgcccga agagcctaaa ggcatccgcg tgatttacgt taccgatatg
     2821 ggcaatgtta ccgattggac gaatcccaat gccgacacgg agtggatgga tgcgaaaaaa
     2881 gccttttacg tcatcgacag cggctttatc ggcggtatgg gtgcggaaga cgcgctgccg
     2941 ttcggcaaca aagagcaggc tgagaaattt gcaaaggata aaggcggtaa ggttgtcggt
     3001 ttcgacgata tgcctgatac ctatattttc aaataatatt gaaaaaaccg gccgaaactt
     3061 taaaaacgag tttcggtcgg tttttctttt cttgttcggt atagtgtcgg caggaaagaa
     3121 ccttcacatc ccgccgtaat tcggcccgct cgcgccttcg gggcaaatcc aagtgatgtt
     3181 ttgcgtcggg tctttgatgt cgcaggtttt gcagtgcacg cagtttgccg cgttgatttg
     3241 caggcgcgga ttgccgtttt cttcaacaat ttcgtacacg ccggccggac aatagcgcgt
     3301 ttcgggcgag gcgtattctt tgtagttcac gtctatcatc gtttgcggat tgttcagcac
     3361 caaatggtcg ggctggtttt cttcgtgcgc gagattggca aggaagacgc tgctcaagcg
     3421 gtcgaaggtc aacacgccgt cgggtttcgg ataatcaatc ggcttacacg cggcggcttt
     3481 tttaagctgc tcgttgtctt tgccgtgatg tttcaaggtc cacggggctt tgcctctgaa
     3541 aatcatctga tcgatgccgg tgtagattga gccgaggtaa acgccccatt tgaatgacgg
     3601 acggacattg cgcgcggcgt aaagctcttg atacagccag ctttgttcaa aacgttgctg
     3661 ataatccgcc gcctctttgc cgctgtcgaa accctccact tcttcaaggt tttccaacaa
     3721 ggggaacacg gcttcggcgg cgagcatggc ggatttcatc gcggtatgaa tgcctttgat
     3781 gcgcggcata ttgaggaaac ccgccgcatc gccgaccaaa atgccgcctt tgaacgagag
     3841 cttcggcaaa ctttgcaaac cgccttcaat cagcgaacgc gcgccgtaag caatgcggcg
     3901 gccgccttca aaggttttgc ggatttcggg atgggttttg aaacgttgga actcttcaaa
     3961 cggcgacaga taaggatttt gatagtccaa accgaccacg aagccgacgg cgactttgtt
     4021 gtcgtcgaaa tggtaaacaa acgcgccgcc gtaggttttg ctgtccagcg gccagcctgc
     4081 gctgtgcacc accaaaccgg gctgatgctg ttcggacggc acttcccaaa cttctttaat
     4141 gcccaagccg taagtttgcg gctggctgtt ttggtcgagt tggaaacgtt cgatgatttg
     4201 tttggaaagc gaaccgcgac aaccttcggc aaacagggtt tgctgcgccc aaagctccat
     4261 gccgggttgg aatgaatcgg tcggctcgcc gtctttgcca atgcccatat tgccggttgc
     4321 aatgcctttg accgaaccgt cttcgtgata cagcacttcg gcggcggcaa agcccggata
     4381 gatttccacg cccatatttt ccgcctgctc cgccaaccag cgcacgactt cgcccaagct
     4441 gacgatgtag ttgccgtgat tgtcgaaatt cggggtaatc ggcaggttga acgctttttt
     4501 ctcggtcagg aacaacactt tgtcctgcgt tactgtgcgt gtcagcggtg cgcctttttc
     4561 tttccagtcg gaaatcaact cattcagcgc aatcggatcg ataactgcgc cagccagcga
     4621 atgcgccccc acctccgaac ctttctccac cacgcaaacg ctgatttcgc gcccgttttg
     4681 ttcggcaagc tgcttgagtt tgatggcggc agacaaaccc gacgggcctg cgccgacaat
     4741 cacgacatcg tattgcatac tgtcgcgggt gatggattct gtcatggcgg ttcctgtgta
     4801 tttattattg aattgcaaat ccgtaattat acaacgggaa catatagtta ccaaatacaa
     4861 caaaggtcgt ctgaaaacca tattttcggt tttcagacga cctttgtcga aatttcaata
     4921 agcacgccac cattttacct gtccgaccgc aaactccgtc tgacgtttcg gactgcgtgt
     4981 gaaaaacgcc ttatccccgc cggcatccct ccctttcggc acaaccgcca aaatcttacc
     5041 tgccaaattt ccctcacggg tttgccaagc atccaaaaac tgcgccctgc tcattgaaac
     5101 atgacccagc gacgggtcgg caagcaaaac cgtattgccg tctataccgc gcaataccga
     5161 gaaatgatca tccttgcggt atttcagata cacgatgacg gggatttgca actgtgcaag
     5221 ctgctcgaaa gacagggcat agcctttcgc ttcaaaaccc aaatcaggca taatgcgccg
     5281 catatcctca aacgacgcgg gcatctgctc cttatccagt ttttttaaca cgtcctcttc
     5341 cgtcagcttt tgcccgtaaa aattgttcaa aagcgtcacc accgaagccg ccccgcagga
     5401 aaaatccaaa tcctgcttta caatattgaa atcgcgcctt tctttccaac tctgcacttt
     5461 gatttttcca taagcaacag g
//
LOCUS       DAWIVB010000144         5460 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWIVB010000144 DAWIVB010000000
VERSION     DAWIVB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.144"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..447
                     /locus_tag="WIX60_001675"
     CDS             <1..447
                     /locus_tag="WIX60_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ5305911.1"
                     /translation="AGRLKKGDTLLSESGAKQTVQNITLKQQPLKAYNLTVADWHTYF
                     VKGNRAETEGVWVHNDCPPRKTPSTPIYGNDSEAYAAAKELGYRKIKERTRNDAAIFK
                     KGKSYISRDVDSHNGGAWKEASSPEKLNRKETRNGTFDKNLNRIGD"
     gene            453..860
                     /locus_tag="WIX60_001676"
     CDS             453..860
                     /locus_tag="WIX60_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ5305912.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASTWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLRRKV"
     gene            882..1259
                     /locus_tag="WIX60_001677"
     CDS             882..1259
                     /locus_tag="WIX60_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ5305913.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            1301..1780
                     /locus_tag="WIX60_001678"
                     /pseudo
     CDS             1301..1780
                     /locus_tag="WIX60_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
     gene            1761..2219
                     /locus_tag="WIX60_001679"
     CDS             1761..2219
                     /locus_tag="WIX60_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305914.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     RTEYYESPDSNLLISVKKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHYPKSYGV
                     DKYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            2226..2282
                     /locus_tag="WIX60_001680"
                     /pseudo
     CDS             2226..2282
                     /locus_tag="WIX60_001680"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2297..2674
                     /locus_tag="WIX60_001681"
     CDS             2297..2674
                     /locus_tag="WIX60_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ5305915.1"
                     /translation="MCELKNFRRNITCFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            2738..3871
                     /locus_tag="WIX60_001682"
     CDS             2738..3871
                     /locus_tag="WIX60_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305916.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESIRTEENLAGTVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTVHGENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFRKESNRIASDSRNSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLENLEKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(3958..4233)
                     /locus_tag="WIX60_001683"
     CDS             complement(3958..4233)
                     /locus_tag="WIX60_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305917.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLMCISVFWIVVFNL"
     gene            complement(4250..5023)
                     /locus_tag="WIX60_001684"
     CDS             complement(4250..5023)
                     /locus_tag="WIX60_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ5305918.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQNNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDNYSTSVGSCINGFT
                     VRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
BASE COUNT         1752 a          972 c         1181 g         1555 t
ORIGIN      
        1 gcaggtcgtc tgaaaaaagg cgacaccctg ctttccgaaa gcggtgcaaa acagacggtt
       61 caaaacatta ccctcaaaca gcagccgctc aaagcctaca atctgaccgt tgccgactgg
      121 catacttact tcgtcaaggg caatcgggcg gaaacggaag gggtttgggt tcataatgat
      181 tgtccgccaa gaaaaacacc atctaccccc atatatggga atgattcaga agcatatgca
      241 gcagcaaagg aattgggata tagaaaaata aaagaaagaa cgagaaatga tgctgctatt
      301 tttaaaaaag gaaagagtta tatatcacgc gatgtagatt cacataatgg aggtgcttgg
      361 aaagaggcat cttcacccga aaaactgaac agaaaagaaa caagaaacgg tacatttgat
      421 aaaaacctaa atcgtattgg agattaatta ttatgtgcga gttcaaggat tttagaagaa
      481 acatcccttg ttttaaggag tatgacgaaa attcatttat tggcaaatgg tatgatgacg
      541 gggtgtggga tgatgaagaa tattggaaat tggagaatga tttaattgag gttagaaaaa
      601 aatatcctta tccgatggat ataccaaggg acatcgtgat tggaatcggt accattattg
      661 attttttaat ggttcaaaat tggaaacttt ttgaaattaa agcttctact tggttgccta
      721 aaagtgtcaa aattaatgag cgttatgaaa ggttcagggt aatgctccgt tatattttta
      781 ctgatctaga tgtagaggac tggaaatttt tttactttcc aatacaacat agtaaaggta
      841 gattgagacg aaaagtttaa ttatattgga gatacattat tatgtgtgag ttcaaggata
      901 ttataagaaa cgttccttat tttgaggggt atgacgaaaa ttcatttatt ggcaaatggt
      961 atgatgacgg ggtgtgggat gatgaagaat attggaagtt ggagaatgat ttaatcgagg
     1021 ttagaaaaaa atatccttat ccgatggaca taccaagata tgttgtcatt ggaatcggta
     1081 ccattattga tttcttaatg gttccaaatt ggaaactttt tgaaattaaa gcttcccctt
     1141 ggttgcctga tagtgtggga attcatgaac gttatgaaag attcacaacg atgctccgtt
     1201 atatttttac cgagaaagac atagtcaacg tgcgatttga ttattacaac aaaaaatagc
     1261 aaaacccggc aatcaaaatg ccgtctgaag cgatatccgg ctttcagacg gcatcggcaa
     1321 cagccaaatc ctgatttcca accgcatcca tccgttttat tcggacggca aatggattaa
     1381 agcggaagat ttgaaagcgg gaagcaggct gttatccgaa agcggcagaa cccaaaccgt
     1441 ccgcaaaacc gttgtcaaac caaaaccgct caaagcctac aatctgaccg ttgccgattg
     1501 gcatacttac ttcgtcaagg gtaatcaggc ggaaacggaa ggggtttggg ttcataattc
     1561 ttgtccgcct aaaagaacag gaagctccaa gaatgaaaaa catggagatg gcggtcgaag
     1621 tcaaatatca gcggaatcaa aaattgctga attaacaaat aaaattattc ccggaatgtc
     1681 caaaaatgaa cgcttaaaga ttaagcaaaa aatcagaaat attgccaaaa atgccaatcg
     1741 aaaaacaaaa ggagaagagc atggtcgacg cggtcgttaa aactcccgaa tttttacctt
     1801 ttacttctgt aggaatattc cgttttggtg cggatataac acagtacaaa aacatactgg
     1861 aaacttttat gtatgaacct cctgatgagt ttagaacaga atattatgaa tctcccgact
     1921 caaatttact catttccgtc aaaaaaaaaa acaaaattat ttcgatattt tgttatcagg
     1981 agctatattt tatgggcgtt aatataatag gtctgaattt tgaagatttt aaacagcttt
     2041 tccattaccc taaatcatac ggtgttgata aatattattt atcaaatgaa tcttatccga
     2101 catatgtcta tgagtttgat gaaataggag tacaagcttg ggaagcaaaa gggaaaattg
     2161 ttactatcat tgccgggggc aaagacaatt attcaacaga accttactac gatgaataac
     2221 attacagaaa agaaacaaga aacggtacat ttgataaaaa cctaaatcgt attggagatt
     2281 aattattatg tgtattatgt gtgagttaaa aaacttcaga agaaatatta cttgttttga
     2341 ggggtatgac gaaaattcat ttattggcaa atggtatgat gacggggtgt gggatgatga
     2401 agaatattgg aagttggaga atgatttaat cgaggttaga aaaaaatatc cttatccgat
     2461 ggacatacca agatatgttg tcattggaat cggtaccatt attgatttct taatggttcc
     2521 aaattggaaa ctttttgaaa ttaaagcttc cccttggcta cctgatagcg taggaattaa
     2581 tgaacgttat gaaaggctta aaacaatgct ccgttatatt tttaccgaga aagacatagt
     2641 caacgtgcaa tttgattatt acaacaaaaa atagcaaaac ccggcaatca aagctcgtct
     2701 gatataatcg atatattctg ttaaacgaaa aataaatatg tttaaaaaat tcaaaccggt
     2761 actgttgtca ttttttgcac ttgtatttgc cttttggctg ggaacgggta ttgcctatga
     2821 gattaatccg cgttggtttt tgagcgatac ggcaactgaa gtacctgaaa atccgaatgc
     2881 ttttgtggcg aaacttgccc gcctgttccg aaatgccgac agggcggttg tcatcgtgaa
     2941 ggaatcgata aggacggagg aaaatcttgc cggaactgtg gatgacggtc cgttgcagtc
     3001 ggagaaggat tatctcgcgc tcgctatccg gctcagtcgt ttgaaagaaa aggcgaaatg
     3061 gtttcacgta acggagcagg aacatgggaa agaggtttgg ctggattacc atatcggcga
     3121 gggcggtttg gttgcggttt cgctttcgca acgctcgccg gaagcatttg ttaatgccga
     3181 atatctgtat cggaacgatc gtccgttttc tgtaaatgtg tacggcggaa cggttcacgg
     3241 ggaaaattat gaaacgacag gagaatatcg ggttgtttgg caaccagacg gttcggtatt
     3301 tgatgcggcg gggcgcggga aaatcgggga agatgtttat gagcattgcc tcgggtgtta
     3361 tcagatggcc caggtatatt tggcgaaata ccgggatgtc gcgaatgacg agcagaaggt
     3421 ttgggacttc cgcaaagaga gcaaccgaat tgcgtcggac tcgcgcaatt ctgtgtttta
     3481 tcagaatatg cgggaattga tgccccgagg gatgaaggcg aacagtcttg tggtcggcta
     3541 tgatgcggac ggtctgccgc aaaaagtcta ttggagtttc gacaatggaa aaaaacgcca
     3601 gagtttcgaa tattatttga aaaacggaaa tctttttatt gcacaatctt cgacggtagc
     3661 attgaaagcg gatggcgtaa cggcggatat gcagacctat catgcgcaac agacgtggta
     3721 tttggatggc gggcggattg tccgcgaaga gaaacaggga gacagactgc ctgattttcc
     3781 tttgaacttg gaaaatttgg aaaaagaggt gcgccgttat gcagaggctg cggcgagacg
     3841 ttcgggcggc aggcgcgacc tttctcactg aacgcaaatg ccgtctgaaa ccggttttca
     3901 gacggcattg gagagtcaaa cgctttttgc aatgccgtat atgccgaatc cgattcgcta
     3961 caaattgaaa accacaatcc aaaaaacgga aatacacatt aaacgggtct ttttaccgac
     4021 cgtatcatcc cataggtttt ccaccccttt tttcatccat aaattcaaac catttaagtt
     4081 tgcatcctta ccgacaattg cagcagcatc atccgcataa cggtcaccca aattagccaa
     4141 ataacgccaa ccgccatagg tatcgccacg ggaagcataa ccacgcaagg tttggatttg
     4201 ttctggtgtt aaaacaactt caccctcatt cataataaga tcctttaaat tattttttac
     4261 ttgaattgtt tacaaaactc tgtaccaatt gctctacttg ttggtgataa cctaccaact
     4321 cctgctgtgt gagctgctgc ttttcccgaa taaacgggta ataccgtacc gtgaaaccgt
     4381 taatacagga acctacgcta gtgctataat tatcttcttc agcctggttt actcgattat
     4441 tccaatgagt aataatcgcc ggattttctc cttttttaat atagtaaaat tcatgaaaat
     4501 atggattttc aggaatatgt cttttatctt cataagcctc gtattcagga taggcagatg
     4561 gcattttttt aaccccgtaa gtaagcaatc tttctcccat agtcgtgctt aaactacacg
     4621 catcttttcg gataacaata tcgtccacgc tatccaaacc atcgcgtaga aattgaataa
     4681 agtgtgcttt gttctgctgg ttgtatggtt cgagcaccca gccttggtcg gtttttttgt
     4741 aatagacgct aatctctatc aaattaatgg agtaagcatc ctgcccgggc gtgtcagaaa
     4801 gccattttgt ggcattgttt tgcgtatacc gtgcaagata gccatagggg ataaccattt
     4861 tggtgccctg aaaatcaaat gtaaccgtat gttcaaatcc tgtcattggc tcgggattgt
     4921 tgaaactggt ttgttcatta acaggttcac atgaggtcat agtaaaaaca ctcccaataa
     4981 acaaaataaa aagttgataa atggtagtag catatggtct cataatttca agcttagaaa
     5041 ttagttaaag aatatattca tggtagatac taactcttta gttaaaaata atttactaac
     5101 ttttgctact taggtataaa ttccaatact taactaaact gagggtaaat atatttatcc
     5161 aagatagaag agaaactttt ttctctgttt ccgcaagaat agttacgggt tgtgaaatat
     5221 gctctatacc caagcagtag atattttcat tgttacttac cgtattgctt catagaattg
     5281 gcactctacg ctttccttta aaaatcttca ataacgatta tcattttttt aaatttattc
     5341 aaaacaatat tcttatacac atctcaagcc cgctgaaata aaacaaagtg gtggttcata
     5401 gatggcaata ccgatttcaa agcagcctgc actttgacaa cacacaagat atacgggaag
//
LOCUS       DAWIVB010000145         5458 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWIVB010000145 DAWIVB010000000
VERSION     DAWIVB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.145"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..>5458
                     /locus_tag="WIX60_001685"
     CDS             <1..>5458
                     /locus_tag="WIX60_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ5305919.1"
                     /translation="ANKITLISTVEQAGIRNQGQWFASAGNVAVNAEGKLVNTGMIAA
                     TGENHAVSLHARNVHNSGTVASQDDANIHSQTLDNSGTVLSSGRLTVRNLGRLKNQNN
                     GTIQAARLDMSTGGLDNTGNITQTGSQALDLVSAGKFDNSGKIGVSDVPQTGLNPNPS
                     VIPQIPSTATGSGSSTVSVSKPGSNNPVSPTAPAKNYAVGRIQTTGAFDNAGSINAGG
                     QIDIAAQNGLGNSGSLNAAKLRVSGDSFNNTVKGKLQAHDLAVNTQTAKNSGHLLTQT
                     GKIDNRELHNAGEIAANNLTLIHSGRLSNDKKGNIRAAHLQLDTAGLHNAGNILADSG
                     TVTTKNNLRNTGKVSVARLNTEGQTLDNTRGRIEAETVNIQSQQLTNQSGHITATEQL
                     TINSRNVDNQNGKLLSANQAQLAVSDGLYNQHGEIATNRQLSIHDKNQNTLALNNADG
                     TIQSAGNVSLQAKSLANNGTLTAGNKLDIALTDDFVVERDLTAGKQLNLSIKGRLKNT
                     HTLQAGHTLKLNAGNIDNQVTGKIIGGEQTDITSEQHVDNRGLINSDGLTHIGAGQTL
                     TNTGTGKIYGNHIALDAQILLNREETTEGSTKAGAIAARKRLDIGAKEIHNQEGALLS
                     SEGIFAVGNRLDEQHHAAGMADTFVNGSAGLEVQGDALMSVRNMQNINNHFKTETYLA
                     KAEKQVRDYTVLGQNTYYQAGKDGLFDNSQGQKDQTTATFHLKNGSRIEANQWHVRDY
                     HIETYKERIIENRPAHITVGGDLTASGQNWLNKDSRIVVGGRIITDDLNQKEITNQST
                     TGKGRTDAVGTQWDSVTKKGWYSGRKRQRRTERNHTPYHDTQLFTHDFDTPVSVIQQN
                     AASPSFQPAASAIKLIDGVSTAAVNGQRIHTGNVVSLNNATVTLPNSSLYTTHPDNKG
                     WLVETDPQFADYRRWLGSDYMLQQLQLDTNHLHKRLGDGYYEQKLVNEQIHQLTGYRR
                     LDGYRSDEEQFKALMDNGLTAAKTFGLTPGIALSAEQVARLTSDIVWMENQTVTLSDG
                     STQTVLVPKVYALARKGDLNTSGGLISAEQVLLKLQNGNLTNSGTIAGRQAVLIQARN
                     INSNGNIQADQIGLKAEKSINIDGGQVQAGRLLTAQAQNINLNGTTQTSGNERNGNTA
                     IDRMAGINVVGSHTEQVDNRTSDGILSLHASNDINLNAATVSNQVKDGTTQITAGNNL
                     NLGTIRTEHREAYGTLDDENHRHVRQSTEVGSSIRTQNGALLRAGNDLKIRQGELEAE
                     EGKTVLAAGRDVTISEGRQITELDTSVSGKSKGILSSTKTHDRYRFSHDEAVGSNIGG
                     GKMIVAAGQDINVRGSNLISDKGIVLKAGHDIDISTAHNRYTGNEYHESKKSGVMGTG
                     GLGFTIGNRKTTDDTDRTNIVHTGSIIGSLNGDTVTVAGNRYRQTGSTVSSPEGRNTV
                     TAKSIDVEFANNRYATDYAHTQEQKGLTVALNVPVVQAAQNFIQAAQNVGKSKNKRVN
                     AMAAANAAWQSYQATQQMQQFAPSSSAGQGQNNNQSPSISVSITYGEQKSRNEQKRHY
                     TEAAASQIIGKGQTTLAATGSGEQSNINITGSDVIGHAGTALIADNHIRLQSAKQDGS
                     EQSKNKSSGWNAGVAVKIGNGIRFGITAGGNIGKGKEQGGSTTHRHTHVGSTTGKTTI
                     RSGGDTTLKGVQLIGKGIQADTRNLHIESVQDTETYQSKQQNGNVQVTVGYGFSASGS
                     YRQSKVKADHASVTGQSGIYAGEDGYQIKVRDNTDLKGGIITSSQSAEDKGKNLFQTA
                     TLTASDIQNHSRYEGRSFGIGGSFDLNGGWDGTVT"
BASE COUNT         1744 a         1403 c         1243 g         1068 t
ORIGIN      
        1 gccaacaaaa tcaccttgat cagtacggtc gagcaagcag gcattcgtaa tcaagggcaa
       61 tggtttgcct cagccggcaa tgtggcagtg aatgctgagg gtaaactggt caacacgggc
      121 atgattgcag cgacgggaga aaatcatgcg gtttcacttc atgcccgcaa tgttcataat
      181 agcggtacgg ttgcctcaca ggatgatgcc aatattcaca gccagacgct ggacaattca
      241 ggtacggtct tatcctcagg tcgattgact gttcgtaatt taggccgtct gaaaaaccaa
      301 aacaacggta cgatccaggc tgcccgctta gatatgtcaa caggtggttt ggataacaca
      361 ggtaatatta ctcaaacagg ttcacaagca ttggatttgg tatctgccgg caaattcgat
      421 aacagtggca agattggtgt aagtgacgtt ccacagaccg gtttgaatcc caatccatca
      481 gtcataccac agattccgag tactgcaaca ggttcaggca gcagcactgt ctcggtatct
      541 aagcctggtt caaacaatcc cgtttcacct acagcacctg caaaaaacta cgccgtagga
      601 cgcattcaaa caacaggagc atttgacaat gcaggatcaa ttaatgcggg tgggcaaatt
      661 gacattgccg cccaaaacgg tttgggaaat tcgggtagtc tgaatgcggc taaactacga
      721 gtatcaggcg attcatttaa caatacggta aaaggcaaac tccaggcaca cgatctggct
      781 gttaacactc aaactgctaa aaacagcggt cacttattaa ctcaaaccgg caagattgat
      841 aaccgtgaac tgcataatgc cggagaaatt gccgccaaca atctgacact cattcattcg
      901 ggccgcttga gcaatgataa aaaaggcaat attcgagctg cacatttaca gcttgatacc
      961 gccggtttac ataatgcagg taacattctt gccgatagtg gaaccgttac caccaagaat
     1021 aatcttcgca atacaggaaa agtttctgtt gcacgactga ataccgaagg tcagactcta
     1081 gataatacgc gcggacgtat agaggctgaa acggttaaca tccaaagtca gcaactgact
     1141 aaccaaagcg gccatattac tgctaccgaa caactgacta tcaatagtcg aaatgtagac
     1201 aaccaaaacg gcaaactcct atctgcaaac caagcacaat tagctgtttc agacggccta
     1261 tacaaccaac atggtgaaat tgccaccaac cggcagttgt ctattcacga taaaaatcaa
     1321 aacactttgg cgttaaacaa tgcggatggc acgattcaat ctgccggtaa tgtatcgcta
     1381 caagccaaat cactcgccaa caatggcaca ttaacagccg gtaacaaact ggatattgct
     1441 ttgacggacg atttcgtcgt agagcgcgac ctcactgcag gcaaacaatt aaatctaagc
     1501 ataaaaggcc gtctgaaaaa tacccatacc ctacaagcag gccatacgct caaactcaat
     1561 gccggcaata tagataacca agttacaggc aaaattattg gtggagaaca aacggacatc
     1621 acatccgaac agcatgttga caacaggggc ttgatcaaca gcgacggttt gacccacatc
     1681 ggtgcaggtc aaaccctgac caacaccggg acaggcaaaa tctatggcaa ccatattgcc
     1741 ctggacgcgc aaatactgct taaccgggaa gaaacgacgg aaggcagtac caaagcgggg
     1801 gcaatagctg caaggaaacg tttggatatt ggagcgaaag agattcataa ccaagaaggt
     1861 gccctactat ccagcgaagg tatttttgcc gtaggtaatc gactggatga acaacatcat
     1921 gcggcaggca tggccgatac ctttgttaat ggcagtgccg gtttggaagt acaaggtgat
     1981 gcattgatgt ccgttcggaa tatgcagaat atcaataatc actttaaaac agagacatac
     2041 ttagccaaag cggaaaagca agtccgcgac tacaccgtac tggggcaaaa tacctactat
     2101 caggcgggaa aagacggttt attcgacaac tcgcaaggac aaaaagacca aactactgct
     2161 acgttccatt taaaaaatgg ttctcgtatt gaggccaacc aatggcatgt ccgagactac
     2221 cacatcgaga cttataaaga acgcatcatc gaaaaccggc cggcacacat tactgtgggc
     2281 ggtgatttga ctgcctcagg tcaaaattgg ctgaacaaag acagccggat tgtagtaggc
     2341 gggcgtatta tcactgatga tttaaaccag aaagaaatta ccaatcaaag tacaacaggc
     2401 aaaggtcgca cagatgctgt cggcacacag tgggattcag ttacaaaaaa aggatggtac
     2461 agcggtagaa aaagacaacg ccgtactgaa agaaaccata ctccttacca tgatacccaa
     2521 ctatttaccc acgacttcga cacgcctgta tccgtcatcc aacagaatgc cgcctcccct
     2581 tcctttcaac ccgccgcatc tgcaatcaaa ctgattgacg gagtatccac ggcagccgtc
     2641 aatggtcagc gcatccatac cggtaatgtg gtctcgttaa ataacgctac tgttactctg
     2701 cctaacagca gcctctatac cacccatcct gacaataaag gctggttggt tgaaaccgat
     2761 cctcaatttg cagactaccg ccgctggttg ggcagcgact acatgttgca acaactgcaa
     2821 ttggacacca atcatctaca caaacggctt ggcgacggct actacgaaca aaaacttgtt
     2881 aatgaacaaa tccatcagtt aacaggctac cgccgactcg acggctacag gagtgatgaa
     2941 gaacaattca aagctctgat ggacaacggc cttactgctg ccaaaacatt cggtctcacc
     3001 ccaggtatcg ccttgagtgc agagcaagtt gcccgcttaa cttcagatat cgtttggatg
     3061 gaaaatcaaa ccgtcaccct gtctgacggt tcgactcaaa ccgtactggt tcctaaagtc
     3121 tatgccctgg cgcgcaaagg tgatctcaat acctccggtg gcctgattag tgccgaacaa
     3181 gtcttactta aactgcaaaa cggcaacctg actaacagcg gtaccattgc ggggcgacag
     3241 gccgtactca tccaggcacg gaatattaac agcaacggta acattcaagc cgaccaaatc
     3301 ggcttaaaag ctgaaaaaag tatcaatatc gacggcgggc aggtacaagc aggcagactg
     3361 ctgactgccc aagcgcaaaa tatcaacctt aacggtacaa cccaaacttc cggtaatgaa
     3421 cgtaacggca ataccgccat cgatcgtatg gccggcatta acgtggtcgg aagccatact
     3481 gaacaagtag ataacagaac ttcagacggc atcctatccc tgcatgccag caacgatatc
     3541 aacctcaatg cggccaccgt ctctaaccaa gttaaagacg gcactaccca aattaccgcc
     3601 ggcaataatc tcaacctcgg caccatccgt accgaacatc gcgaagccta tggtacatta
     3661 gatgacgaga accatcgcca tgtccgccaa agtaccgaag tcggcagcag tatccgcacg
     3721 caaaacggcg cactgcttag agccggtaac gacttaaaaa tccgccaagg cgaactggag
     3781 gccgaagaag gcaaaaccgt ccttgccgca ggacgtgatg tcactatcag cgaaggacgc
     3841 caaataaccg aactggatac ctcggtaagc ggaaaaagca aaggcatcct ttccagtacc
     3901 aaaacacacg accgctaccg cttcagtcat gatgaagcag tcggcagcaa catcggcggc
     3961 ggcaaaatga ttgttgcagc cgggcaggat atcaatgtac gcggcagcaa ccttatttct
     4021 gataagggca ttgttttaaa agcaggacac gacatcgata tttctactgc ccataatcgc
     4081 tataccggca atgaatacca cgagagcaaa aaatcaggcg tcatgggtac tggcggattg
     4141 ggctttacta tcggtaaccg gaaaactacc gatgacactg atcgtaccaa tattgtccat
     4201 acaggcagca ttataggcag cctgaatgga gacaccgtta cagttgcagg aaaccgctac
     4261 cgacaaaccg gcagtaccgt ctccagcccc gaggggcgca ataccgtcac agccaaaagc
     4321 atagatgtag agttcgcaaa caaccggtat gccactgact acgcccatac ccaggaacaa
     4381 aaaggcctta ccgtcgccct caatgtcccg gttgtccaag ctgcacaaaa cttcatacaa
     4441 gcagcccaaa atgtgggcaa aagtaaaaat aaacgcgtta atgccatggc tgcagccaat
     4501 gctgcatggc agagttatca agcaacccaa caaatgcaac aatttgctcc aagcagcagt
     4561 gcgggacaag gtcaaaacaa caatcaaagc cccagtatca gtgtgtccat tacctacggc
     4621 gaacagaaaa gtcgtaacga gcaaaaaaga cattacaccg aagcggcagc aagtcaaatt
     4681 atcggcaaag ggcaaaccac acttgcggca acaggaagtg gggagcagtc caatatcaat
     4741 attacaggtt ccgatgtcat cggccatgca ggtactgccc tcattgccga caaccatatc
     4801 agactccaat ctgccaaaca ggacggcagc gagcaaagca aaaacaaaag cagtggttgg
     4861 aatgcaggcg tagccgtcaa aataggcaac ggcatcaggt ttggaattac cgccggagga
     4921 aatatcggta aaggtaaaga gcaaggggga agtactaccc accgccacac ccatgtcggc
     4981 agcacaaccg gcaaaactac catccgaagc ggcggggata ccaccctcaa aggtgtgcag
     5041 ctcatcggca aaggcataca ggcagatacg cgcaacctgc atatagaaag tgttcaagat
     5101 actgaaacct atcagagcaa acagcaaaac ggcaatgtcc aagttactgt cggttacgga
     5161 ttcagtgcaa gcggcagtta ccgccaaagc aaagtcaaag cagaccatgc ctccgtaacc
     5221 gggcaaagcg gtatttatgc cggagaagac ggctatcaaa tcaaagtcag agacaacaca
     5281 gacctcaagg gcggtatcat cacgtctagc caaagcgcag aagataaggg caaaaacctt
     5341 tttcagacgg ccacccttac tgccagcgac attcaaaacc acagccgcta cgaaggcaga
     5401 agcttcggca taggcggcag tttcgacctg aacggcggct gggacggcac ggttaccg
//
LOCUS       DAWIVB010000146         5429 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWIVB010000146 DAWIVB010000000
VERSION     DAWIVB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.146"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(78..278)
                     /locus_tag="WIX60_001686"
     CDS             complement(78..278)
                     /locus_tag="WIX60_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305920.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            428..1567
                     /locus_tag="WIX60_001687"
     CDS             428..1567
                     /locus_tag="WIX60_001687"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ5305921.1"
                     /translation="MSQNASVGIVMPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIEQWAAIVGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGFGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGDSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            1564..2145
                     /gene="metW"
                     /locus_tag="WIX60_001688"
     CDS             1564..2145
                     /gene="metW"
                     /locus_tag="WIX60_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ5305922.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTNSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRILERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            2262..3098
                     /locus_tag="WIX60_001689"
     CDS             2262..3098
                     /locus_tag="WIX60_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ5305923.1"
                     /translation="MKNKTSSLLLWLTAIMLTACSPSKDDKTKEVGASAASSSASSAP
                     SQTDLQPTASAPDNVKQAESAPPSNCTSLHPATGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQNALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYISQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNTG
                     RI"
     gene            complement(3149..3709)
                     /gene="efp"
                     /locus_tag="WIX60_001690"
     CDS             complement(3149..3709)
                     /gene="efp"
                     /locus_tag="WIX60_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ5305924.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(3752..4900)
                     /gene="earP"
                     /locus_tag="WIX60_001691"
     CDS             complement(3752..4900)
                     /gene="earP"
                     /locus_tag="WIX60_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ5305925.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDTEALRERLMLPEKNASEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDQLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSAPEKLAAFVSKHQKIR"
     gene            complement(4919..5197)
                     /locus_tag="WIX60_001692"
     CDS             complement(4919..5197)
                     /locus_tag="WIX60_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305926.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         1376 a         1400 c         1419 g         1234 t
ORIGIN      
        1 cacctcctac cgcaagccct tttcatcaaa tatgccgtct gaaacagaaa aatgcgtttc
       61 agacggcatt ttttccgtca atacgaatcc ggaatgtgtt tgaaatacag cataaacaga
      121 taccaaaaca gcatcgcggc ggctgcaccg agcagtaccc atgcgctgat ttcggcgact
      181 tcgcgcaggg cgcgtttttg cctgcggctg aaaaggcggt tgataatcaa cgcggcaagt
      241 gcgaaaaaga aaaaaatacg gaaaagtctg cctatcatgt cgggaagcgg tggaggtcgt
      301 ctgaaaaagt atgattcgtg tgcggcggat gagggtcaaa cgcgtccggc tgcttgtccg
      361 accgtgtcaa aaagtcatgc cgtttgcgat ataataacgc ttttcgccgc atttgttgga
      421 acaggatatg agtcaaaatg cctcggtggg cattgtaatg ccccaaaaaa ttccgtttga
      481 gatgccgctg gttttggaaa acggtaaaac tttgccgcgt ttcgatctga tgattgaaac
      541 ctacggcgag ctgaatgctg aaaaaaacaa tgcggtttta atctgccacg cgctgtcggg
      601 caaccatcat gttgcgggca ggcattcggc ggaagataaa tatacgggct ggtgggacaa
      661 tatggtcgga cctggcaaac cgattgatac ggaacgtttt ttcgtggtcg gtttgaataa
      721 tctgggcggc tgcgacggca gcagcgggcc tttgtcgatt aatcctgaaa cgggcaggga
      781 atacggcgcg gattttccgg tggttacggt gaaggactgg gtaaaatccc aagccgcgct
      841 tgccgactat ctcggtatcg aacaatgggc ggcgattgtc ggtggcagct tgggcggtat
      901 gcaggccttg cagtggacga tttcctatcc cgagcgcgtg cgccatgctt tagtgattgc
      961 gtctgcgccg aaactgtcca cgcaaaatat cgcgtttaac gatgtggcgc gtcaggcaat
     1021 tttgaccgac cctgatttca atgaaggaca ttaccgcagc cacaataccg ttcctgcacg
     1081 gggcttgcgg attgcccgca tgatgggaca catcacttat cttgccgaag acggtttagg
     1141 caaaaaattc ggacgcgatt tgcgttccaa cggttatcaa tacggctttg gtgttgaatt
     1201 tgaagtggaa tcctatcttc gctatcaggg cgacaaattc gtcggacggt tcgacgccaa
     1261 cacttacctg ctcatgacca aggctttgga ctatttcgat ccggcggcag atttcggcga
     1321 cagcctgacc cgcgccctgc aaaatgtgaa agcgaaattc tttgtcgcca gcttcagcac
     1381 cgactggcgt ttcgcacccg aacgttcgca cgaactggtc aaggcactga ttgccgccca
     1441 aaaatccgtg cagtatatcg aagtcaaatc cgcacacggg cacgatgcct ttttaatgga
     1501 agacgaagcc tatatgcgtg cggtcgccgc ctatatgaac aacgtttata aggaatgtca
     1561 gcaatgaacc tgcgcgatga tttgcaactg atttacgacc ggatacccga aggcagccgc
     1621 gtcttggact tgggctgcgg cgacggcgaa ctgcttgccg cattggtcga acacaaaaaa
     1681 tgcagcggct acggcatcga aatcgacaca aatagcgtca tcgccgcgat gtcgcgcggc
     1741 gtaaatgtta tccaagccga tttggaagaa ggtttaaccg cattcaacga tcaaagtttt
     1801 gatgtgattg tgttgagcca aaccatccaa gcgatgcaga ataccgaaaa aatcctgcgc
     1861 tgcctgatgc gcgttgccaa acaggcgatt gtcagcttcc cgaatttcgg ctactggcgc
     1921 aaccgcgtcc aaatcgcact cggcggacat atgccggttt ccgaacgtat gccctaccat
     1981 tggtacgaca cgcccaatat ccattggtgc acgctcaaag actttgattt attgtgcgcc
     2041 aaaaacaaca tccgcatcct cgagcgcgcc gtgatgacgg gcaacagaca ggtcaaacat
     2101 ttccccaacc ttttgggcag cctggcgttt taccgcgtcg ggtaagacag gttgagaacc
     2161 ttgtcggcat taacaggttc ttcggttcta ttaacaacag gccgtctgaa aaatgaaacg
     2221 gtaggttttc agacggcctc tttaatactg gaaacaatag aatgaaaaac aaaacctcat
     2281 cacttctctt atggcttacc gcaatcatgc tgaccgcgtg ttctccgagc aaagacgata
     2341 aaaccaaaga agtcggtgca tccgctgctt cgtcctccgc gtcatcagct ccttcccaaa
     2401 ccgatttgca accgaccgca tccgcccctg ataacgtcaa gcaggcagaa agcgcgccgc
     2461 cgtcaaattg caccagcctg caccccgcca ccggcattga cgatctcatg cagcaaatcg
     2521 ccgaacacat tgactcggac tgtctgtttg ccctttccca tcacgaactg gaaacccgtt
     2581 tcggcttacc cggcggtggc tatgacaaca tacagcggct gctgtttccc gacatccgcc
     2641 ctgaagatcc cgactaccat cagaaaatca tactggcaat tgaagacttg cgttacggaa
     2701 agcgcacgat cagccggcag gcacaaaatg ccttgatgga acaggaacgc cgcctccgag
     2761 aagcgacgct gttgctgata cagggcagtc aagaaacccg cggacaaggc gaggagccga
     2821 aacgcacgcg ttattttgaa gtttcggcaa cccctgccta ttcgagccgg cacaacaacg
     2881 gacttggcgg caatttccaa tacatcagcc aattgcccgg ctatctgaaa atacacggag
     2941 aaatgcttga aaaccaatca ctcttccggc tgtccaaccg tgaacgcaat cccgacaaac
     3001 cgtttttaga catccatttt gacgaaaatg gcaaaatcac gcgtattgtc gtttacgaaa
     3061 aaaacatcta cttcaatcca aacacggggc gaatataaaa aaaatgccgt ctgaaacgtt
     3121 ttcagacggc atttttcaat gcggcaaatc aggcgcgttt gcggaattcg ccggtgcggg
     3181 tgtcgatttc gactttatcg ccgttttcga tgtaagacat cacttggatt tcggtgccac
     3241 cgaccaggcg ggcggttttc atcactttgc cggaagtatc gcctttgacg gcaggctcgg
     3301 tgtactcgac ttcgcgcacg atgatggtgg gcagttctac ggagataggg ttgccttcgt
     3361 agaaggttac ttcgcattgg tcttccatac cgtcaacgat gaatttcaac gcgtcgccga
     3421 tgttgtcagc ttcgatttcg tattggttga attcttcgtc cataaagacg tacatcgggt
     3481 cggcaaagta gctgtacgta cagtttttgc gggacaggat gaccacgtcg aatttgtcgt
     3541 cggctttgta aatggtttcg gaagccgcgc cggtcagcag gtttttcagt ttcatgctga
     3601 ctttggcgga agagcggccg cctttgatgt attcggtttt ttgaacgacc ataggatcgt
     3661 tgccgaccat aaatacattg ccggcgcgca gttcttgtgc tgttttcatt gtgattttcc
     3721 aatcaaatcg gttgtcgtaa aacgcgctat tctagcgtat tttttgatgc tttgaaacaa
     3781 aggcagcgag tttttcagga gctgacggct gcccgaaaag ataacggctc caatcctccg
     3841 cgccttgccg ccagccgttt tgatgttgtt gcagggtttg ccaacattcg aggcgttgtg
     3901 ttgcggataa agcctctccg ccgttgaggt cgtccgaaag acggcggtgt gccgacacgg
     3961 tttcgggcgt gtagaaaccg tgtgccttat cccaaaaggc gtggagtttg tcgagatgga
     4021 cattctcgtc ttgcgggtag atgtgccaaa agaagggttt gcccgcaagc tgggcgcgca
     4081 cgaaactgtc ttcgccgcgg atgacggcgc agtcggcaag gtgcagcagt tggtcgaagt
     4141 cctgttgcgg cacgaaaggg attttgacga ggcggacgga tgccgtctga aaaacatcgc
     4201 cgtcgttttg cagggcatct tgcggaataa cgccgctttg tttgaggctg tcgatgattt
     4261 gcgtccccgc cagcaacagt gtcatcgggc tgcctgcctg tcgccacatt tccagccact
     4321 ttgcccaaac atcgctccga tagccgaaaa gcagccattc ggaggcgttt ttttcgggca
     4381 gcatcagccg ctctcgcagg gcttcagtat cgaaacggac ggcttcgcag taatcacgtt
     4441 cgcgtatcaa cccgccgctt ttttcgctga aacccataaa ccaaaaatat ttttgaacac
     4501 cctcctgcgg cgaaggcatc agatgcagcc tttcattgct ttcctccgcg ctcaaatatt
     4561 cccaattcag ccaaagcggc ttgtgtcggc ggataatgtg cagcacattt tcgggcaggt
     4621 cgcaggcaaa agtttcgatg acgacatcgg gaacaggcgc ggtatcaata tctgccgcat
     4681 cggaatgcca agtgcggaca tgaatatcct gatgaacgca gggaacatcg ggcaaatcag
     4741 ggcaaagcgc acgcaaggcg gacacatcgt ccgtccacaa atgcacctgc caaccgagtt
     4801 cgcggtgcaa aacacgggcg agccgccacg aaacgccgat gtcgccgaaa ttgtcgatga
     4861 ccttgcaaaa aatccaacag acaaaaggag gagtattcat agaaataacg gaaacgggct
     4921 aaaacgcgcc aaaatagtgg aacaaataca caatcagcaa aataatcgga ataaacagca
     4981 acatagtttg acgtttcaaa tcaaactctt gtttcaaatg atgcttgatg gtgtcgaaaa
     5041 ggacgaattt gaacaaaaac gtcgcttcgc aggcaaggat gatgccgtta atcaaatgct
     5101 ggggtttgtc ttcgggcgga ttggcgacga aaacggcaag cgtcgccaag gggatgaggc
     5161 agacgacgag ccagcggaca agcaggtttc gggacattgc aaggctttca aaaaagcaaa
     5221 acgccattat cgccccaagc gcacgccttg tctaacggac gcaccagaga tgccgtctga
     5281 agccttgccg caggaaaacg ccgcaccgcc gaacctttaa atacgcccgg gacgggcatt
     5341 ccccaaaaaa tcggacactg ccgctgatgc ctgcggacaa ggcggcatcg ggcgcgggca
     5401 tattgaaaac ccggcgcgtc ggtttgaaa
//
LOCUS       DAWIVB010000147         5403 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWIVB010000147 DAWIVB010000000
VERSION     DAWIVB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.147"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(247..1506)
                     /gene="rho"
                     /locus_tag="WIX60_001693"
     CDS             complement(247..1506)
                     /gene="rho"
                     /locus_tag="WIX60_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ5305927.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1726..4110)
                     /gene="ppsA"
                     /locus_tag="WIX60_001694"
     CDS             complement(1726..4110)
                     /gene="ppsA"
                     /locus_tag="WIX60_001694"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ5305928.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4522..5343
                     /locus_tag="WIX60_001695"
     CDS             4522..5343
                     /locus_tag="WIX60_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ5305929.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1141 a         1266 c         1588 g         1408 t
ORIGIN      
        1 acaaaaatga gtgcggttcg gcgcaacctt gaatcaagtt cccgcatcgg ttttcattgc
       61 cggtacggat gcgttcaagc cggctttgca aaggccgcgc tttcggcaag cggacacgga
      121 cactgccgac ggttgcgccg ttaacggggg gagggaggag ctgcgccgac cgtgtgaatg
      181 aaagtgccgt ctgaaacccg attttcaggc ttcagacggc atttcgcatt aatgcgggcg
      241 gcgcgtttat ttgccgcgca tcagttcaaa gaaatcgtcg ttgtttttag aggctttgat
      301 tttcccgatt aaaaattcgg ctgcctcgat ttcgtccatc gggtgcagga acttgcgtaa
      361 gagccacata cgttgtaact ggtcgtttgg gacaagcagc tcttcgcggc gcgtgccgga
      421 tttgttgatg ttgatggcgg ggaagaggcg tttttccgcc atacggcggt caaggtgcaa
      481 ttccatattg ccggtgcctt tgaattcttc gtaaatcaca tcgtccatac ggctgccggt
      541 ttcaaccaat gcggtggcga tgatggtcag cgaaccgcct tcttccacgt tgcgcgccgc
      601 gccgaagaaa cgtttgggac gatgcagcgc gttggcatcg acaccgccgg tcaggatttt
      661 gcccgaggta ggcacgacgg tattgtaggc gcgggcaagg cgggtaatcg aatccagcag
      721 gatgaccacg tcttttttgt gttccaccat acgcttggct ttttcaagca ccatttcggc
      781 aacttggacg tggcgttgag ccggctcgtc aaaggtggag gagactactt cgccacggac
      841 ggagcggctc atttcggtta cttcttcggg acgttcgtca atcaagagga cgatgagttc
      901 gacttcggga tagtttgcgg taacggcgtg ggcaatgttt tgcagcatca cggttttacc
      961 gcttttgggc ggggcaacca agagggcgcg ctgacctttg ccgatagggg aaatcaggtc
     1021 gatggcacgt ccggtcaggt tttcttcgga ctttaagtcg cgttccagct tcaactgttc
     1081 ggtcggaaac agcggggtca ggttttcaaa caggatttta tggcggcata cttccgggtg
     1141 gtcgccgttg atggtatcaa gcctgaccag ggcaaaatag cgttcgttgt cttttgggac
     1201 gcgcacgctt ccttcgatgg tgtcgcccgt atgcaggttg aagcggcgga tttgggtggg
     1261 cgagacatag atgtcgtcgg ggccggcaag ataggacgtg tccgcgctgc ggaggaagcc
     1321 gaagccgtcg ggcaggattt caagcgtgcc ggagcaggtg aaaccctcgc cttttttcat
     1381 catctggcgg acgatggcaa atacgaggtc ttgtttgcgg aatcggttgg cgttttcgat
     1441 gccgtgttct tccgccaatt ctaagagttt ggaaatgtgc agggtttgta attcggagac
     1501 gtgcataata atgatgtatt ttgaagagga aaaagacagg cagatgccgt ctgaaagaag
     1561 aagctgaccg ttgccggttg ctcggggaag ggggaattgt aggcagtcgg cgcgtgggtg
     1621 tcaaatatta tcgcggacgg ggcatcggca ggaaatgccg tctgagcgga gctgcttgga
     1681 aaaaaatacc cccgcgcttt tcaggctcgg gggtatgggc attgattatt tgttcaattc
     1741 attcgccaaa tatagccaag tttcgatgac ggtatccggg ttcagggaaa cgctttcaat
     1801 gccttcctca accagccatt tggcgaagtc cggatggtcg gacgggcctt gaccgcagat
     1861 gccgacatat ttgttctgct tgcggcaggc ggagatggca aggtgcagca tcactttgac
     1921 ggcagggttg cgttcgtcaa acgattcgga taccaagccg ctgtcgcggt cgagaccgag
     1981 ggtcagttgg gtcatgtcgt tcgagccgat ggagaagccg tcgaagtatt gcaggaattg
     2041 ttccgccaat accgcgttgc tcggcagctc gcacatcata atcaggcgca ggccgttttt
     2101 gccgcgttcc aagccgtttt ctttcagggc tttgacaacg gcttcggctt cgcccaaagt
     2161 gcggacgaac ggaatcatga tttcaacgtt ggtcaacccc atttcatcgc ggacgcgttt
     2221 caaggctttg cattccaagg cgaaacagtc tttgaagttg tcggcgacat aacgcgccgc
     2281 accacggaag cccaacatcg ggttttcttc atgcggttcg tatacgttgc cgccgaccag
     2341 gttggcgtat tcgttggatt tgaagtcgga catacggacg atggttttac gcggataaac
     2401 cgatgcggcc aatgtcgcca cgccttcggc gattttatcg acgtagaagt cgacagggga
     2461 cgcgtaaccg gcgatacggc gggtaatttc cgcttttaat tcgtcgtctt gtttgtcaaa
     2521 ttccaacaag gctttggggt ggataccgat ttggcggttg atgataaatt ccatacgcgc
     2581 caagccgatg ccttcgctgg gcaggttggc gaagctgaat gcgagttcgg gattgccgac
     2641 gttcatcatg acttttacag gtgctttagg catattgtct aaggcgacat cggtaatctg
     2701 tacgtccaac agaccggcat agataaagcc ggtatcgcct tcggcacagg atacggtaac
     2761 ttcttgaccg tttttcagca attcggttgc attgccgcag ccgacaacgg caggaatgcc
     2821 caattcacgc gcgatgatgg cggcgtggca ggtacggccg ccgcggttgg taacgatggc
     2881 agaagcacgt ttcatcacgg gttcccaatc cggatcggtc atgtcggtaa cgagtacgtc
     2941 gccggcttcg acggaatcca tctcggaagc atctttaatc aggcgcacct tgccctgacc
     3001 gactttctga ccgatggcgc ggccttcgca taatacggtt ttgtcgccgt tgatggcgaa
     3061 gcggcgcagg ttgcggttgc cctcttcttg ggattttacg gtttcgggac gggcttgcag
     3121 gatgtagagt ttgccgtcca agccgtcgcg tccccattcg atatccatcg ggcggccgta
     3181 gtgtttttcg atggtcagtg cgtaatgcgc caactcagta atttcttcgt cggtaatgga
     3241 gaagcggttg cggtcttcct cggggacatc gacgttggtt acggatttac cggcttctgc
     3301 tttgtcggta aaaatcattt tgatgtgttt tgaacccatg gttttacgca ggatggcggg
     3361 cttgcccgct ttgagcgtgg gtttgaacac ataaaattcg tccgggttga ccgcaccttg
     3421 tacgacgttt tcgcccagac cgtaagagga ggtaacaaag acgacttgat cgtagccgga
     3481 ttcggtgtcg agggtgaaca tcacacctga tgcgccgctg tcggaacgca ccatgcgttg
     3541 aacgccggcg gaaagggcga cgatgtcgtg ttcgaagcct ttgtggacac ggtaagaaat
     3601 ggcacggtcg ttatacaggg aagcgaatac atggtgcatc gcttctttaa cgttatccaa
     3661 gccgttgatg ttcaagaagg tttcctgttg tccagcgaat gatgcgtccg gcaggtcttc
     3721 ggcagttgcg gaagaacgta cggcaacgga aatgtccgca ccgccggcat cggcaaccat
     3781 tttgttccat gccgcttcga tttcggcatc gagctgttcg gggaaaggcg tatccaaaat
     3841 ccattggcgg atttctttgc cgacgcgtgc cagttcggca acgtcttcga catccaattt
     3901 tgccagtgcg gcggaaatgc gttcgctcag accgttgtgt gcgaggaatg cgcggtaggc
     3961 ttcggccgtg gtggcaaagc cgccggggac gcgaacgcct ttttcggtca gctgactgat
     4021 catttcgccc agcgaggcgt ttttaccgcc cacgcgttca acatctgtca tacgcaggtt
     4081 ttcaaaccag attacgtagt tgtcggccat ttgtgtgtcc aatccaaaat atgttaaaaa
     4141 agaaacaaat ccgcgtgctt attttaagcg attcgttcct ctgctgtcat gtgttttatc
     4201 cgttttaaaa tcatgatgcc gtctgaaaaa ttgcggtttc ggcgtgtgta gcggtttgaa
     4261 acttacagcc ggtatacttc tttttttggg tattttcttt gtaaaacagg tggtttgaat
     4321 aggttaatgt tttttctgtt tgattttttt gtttattttt taaaattttc tgccaaaaaa
     4381 tactttatat aaataatttt atttcaaaat tatattgtgt ctgtttgggt gtaatccgag
     4441 gtaggtgtgc tgcgggtgct ttccttgtgt ctgctgctgc tgttatgatg ggattttaaa
     4501 cctgtgtttt aaggatggaa gatgagcagt ccgcgccatg tgttttacat ttccgaccgt
     4561 accggtctga ctgctgagaa tatcggcgag gcgttgctga accagtttgg caatctgtcg
     4621 ttcaaacgcc atacgcatcc gtttgtcgat acgccggaaa aggcgcgcgc ggtggtggag
     4681 aaggtcaatc ggagccggca ggaaaacggt cagcgtccga ttgcgtttgt cagtgtggtt
     4741 gatgacgaaa tccgtcggat tatcaaaggg gcggatgctt ttcagattaa tttctttgag
     4801 acttttttgg gactgttgga gaaggaactc aataccgaag ccacggcatc cgggcagggg
     4861 catcacagta tcggtaatac gaagcgttat gatgcgcgta tggaagcggt caatttttct
     4921 ttgaaccacg acgacggggt cagcgataag aaccttcagg aagcggatgt aatcttgatg
     4981 ggtgtatcgc gttcgggcaa aacgccgacc tgcctttacc tcgccctgca atacggcatc
     5041 cgtgcggcaa actatccgct gattcccgac gatttggaat cggccgatct gccgcgtatg
     5101 gtcaagcctt atagggataa gctgttcggg ttgaccatcc agccggaacg tttgcaggcc
     5161 atccgccaag agcgccgccc gaattcaact tatgccaaaa tcgatacatg ccgcagcgag
     5221 gtggcggacg cgcagagtat gttcagacgg catgggattc cgtttgcgaa tacgacggat
     5281 aagtcggttg aggaattggc ggtacacatc cttcaggcgt gcaagctcaa acgcaggttt
     5341 tgacgggctt tgattcggtt tgaaggcgga actgccgtct gaaatcaggt ttcagacggc
     5401 agt
//
LOCUS       DAWIVB010000148         5330 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWIVB010000148 DAWIVB010000000
VERSION     DAWIVB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.148"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            304..1737
                     /locus_tag="WIX60_001696"
     CDS             304..1737
                     /locus_tag="WIX60_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ5305930.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFQIKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALASLAYEAGIPQDLLPVVSGSASEIGHEFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSAIYDEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGASCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1846..2634
                     /locus_tag="WIX60_001697"
     CDS             1846..2634
                     /locus_tag="WIX60_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ5305931.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3002..4558
                     /locus_tag="WIX60_001698"
     CDS             3002..4558
                     /locus_tag="WIX60_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ5305932.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4575..5321
                     /gene="surE"
                     /locus_tag="WIX60_001699"
     CDS             4575..5321
                     /gene="surE"
                     /locus_tag="WIX60_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ5305933.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAVFWHAD"
BASE COUNT         1247 a         1353 c         1519 g         1211 t
ORIGIN      
        1 gttcggattt ttgccatact ctaccacacg ttgcaactgc aatctttgct ccttatatat
       61 tgcaccccat caaagggctg cattactttt ctttctaaag attactatcg tataaaaaat
      121 atttttctta aacgacaggg attgcgcccg cgccatattc agacaccgcc cggatgttgc
      181 gctgcccgat cggatgcttc agacggcatc ggaagggttt gcagttttgg aatatgaaga
      241 tgataatgtc cgcgagattg acggcatttg aaagcggtta ttccgcatac cggaggatac
      301 gaaatgaacg aatattccca attaatcaag catcccgata tttcccttcc cccggtttca
      361 gacggcatca aagtcgataa tccggcaacg ggcgagactt tggcgtttgt ccgcaagacg
      421 gattcggaca agctgaaaaa cctgattcaa aaagcagctg cagcacaaaa attatgggcg
      481 gcaaaaactg cgttggaacg cgccgatgtg ttgtggcgtt ggtattttca gattaaagaa
      541 aacaaagaag cattagcgcg cctgatgacg atggagcagg gcaaaagcct gaccgaggcg
      601 cgtggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgccgaaga ggcgcggcgg
      661 attgacggcg atgtgctgac gagtgtgaaa gcgtcgcaaa aactggtcgt gttgaaacag
      721 cccgtcggcg ttaccgctgc gattacgccg tggaactttc cgtccgcgat gattgcgcgc
      781 aaggccgcgc ctgctttggc ggtgggttgc gcgatgatcg tcaaacccgc atcgctcacg
      841 cctttgagtg cgtatgcctt ggcttcgttg gcttacgaag cgggcatacc gcaggatttg
      901 ttgcctgttg tcagcggcag tgcttcggaa atcggccatg aatttgctac gaacccgatt
      961 atccgcaaaa tcagcttcac cggctcgacc gaagtcggcg caaaaatttt tgccgacagc
     1021 gcggcggaca ttaaaaaact cagtttggag ctgggcggca acgcgccgtt tatcgtgttt
     1081 gacgatgccg atttggacaa agccgtcgaa ggcgcgctcg ccagcaagtt ccgtaacagc
     1141 ggtcagacct gcgtctgcac caaccgcgtt tacgctcaat ccgccattta cgacgaattt
     1201 tgccgcaaat tgagtgaaaa agcagccgcg ctcaaattgg gcaacggctt ggaggatggt
     1261 gtgaaccaag ggccgctgat tgaggaaaaa gcggtggaga aagtcgagca gcacatcgcc
     1321 gacgcgcttg ctaaaggtgc aagctgcctg accggcggca aacgcagcgc gttgggcgga
     1381 acgtttttcg aaccgactgt tttaagcggc gtaacggcgc aaatggcggt ggcacgcgaa
     1441 gaaaccttcg ggccgttgtg tccggtattc cgttttgaaa ccgaagccga ggtcatcgag
     1501 gctgcgaaca atacggaata cggtttggca gcttaccttt tcaccgccga caccgcccgc
     1561 caatggcgcg tcggcgaagc cttggaatac ggtatggtcg gcatcaatac gggcttaatc
     1621 agcaatgaag cggcaccgtt cggcggcgtg aaacgtagcg gtttgggacg tgaaggcagc
     1681 aaatacggtg cggacgaata tctagaattg aaatatctgt gtatggatgt cgggtgacgg
     1741 atgccgtctg aacggcagga tttcagacgg cgccgcattt taagcagtct ctatctgttg
     1801 tacaatgcgc cctgttttta cggttatttt tgatttgaac aagatatgat ggaaaacgga
     1861 aaaacagtcc cgagatggcg ttttgccttg aaaagtgcgg gctggcacct cttaatcagc
     1921 ctgtcggttg cagggctggc ggcattgctg gtttttaagg tttggtatcc ttatccttat
     1981 gccgagctga cgggagggct gtcgctttat cagctggtgg tggctgtcga tattgtatgt
     2041 ggtccgctgc tgactttaat tttggcaagc ccgaagaaaa agacaaaggc acgcatggtc
     2101 gatttttcca tggtcggcat catccagctg gcggctttgg tgtacggtct gcacagcgtt
     2161 tcgctggcgc gtcctgtggt ggaagcgttt gaacaggatc gtatgaccat tgttacggcg
     2221 gcggaagtcg tggtcgaaga tttgcacaaa gcccccgaag ggctgcaaag cctgtcgtgg
     2281 ttcggcatcc gccgcattgc attgaaagaa cctgaggatg cggatgagaa gaacaagacg
     2341 ctggatttgt ccctgaaagg tatcgagccg agtatgcgtc ccgaccagtg gctgccgtat
     2401 tccgacaagg aagcagaaga aatccgcaaa catctgaaac cgctgaaagt cttggcggat
     2461 gcgagaaaaa cgacggttgc ggacattctg aaacaggcag gtctcgccga aggggaggag
     2521 ctgtattacc tgccgtttac cagcagcagg cagaaagagt ggatagtcat taccgataaa
     2581 gagggcaaca ccaaaggcta cgcgccgata gacggcttca tcatcacccc ttaagcgttg
     2641 ggactccgtc cgcactcgaa catccgttct tcgcggcggt agaatcagac tgtatttgag
     2701 aggggcgcaa cccctcaatc agggcaacac ataggggaag gctttatgtg ccgtccgtgt
     2761 gttgaaacat ttcaaaataa gctcatgccg tccgaacatc ctttcagacg gtatggcgtt
     2821 ttgccattgc cccgataagc tgttaaacta tttaaattat ttcgaccgaa ggtacacgca
     2881 catgcaagac cccgagcaga gcagcaagcc cgcccgccgt tttttgtgcg taccgtctgt
     2941 tcaggcagct tgagcgcttc aggctgcctt ttcgcgtttg aacttgagga atacgaaaat
     3001 catggatttc agttggttgg cagaaccgca tacctggata ggttttgcca cgcttttggt
     3061 gttggaagtc gtattgggga tagacaatct tgtctttgtg gcgattttgg caaacaaggt
     3121 ccagcccgca cggcgcgacc gcgcacggat tatcgggctg gggctggcag tcgtcatccg
     3181 catcattatg cttgctttta tggcgcacat catcacgctg accgagccgc tgttccaaat
     3241 cggcggcctc gccgtttccg gcaaggacat gattatgctc gcgggcggta ttttcctgct
     3301 ttacaaagcc accaccgaac tgcatgaacg cctcgaaggg cacaaccgtt ttaccgttgc
     3361 cgacagccaa aaaaaacacg cgccgttttg gggcgtggtc gcgcaaatcc tgatactgga
     3421 tgccgtgttt tccatcgatt cggtcattac tgcggtggcg atggtcgatc atatcgtcgt
     3481 ggcgatgggt gcggtcgtcg tcgcgatggc tgtaatgatt tctgccagca aactcttgac
     3541 cgaatttgtc gacagacacc ctaccgtcgt gatgctctgc cttggttttt tgttgatgat
     3601 cggtttcagc ctgattgccg aagccttcca tttccacatt cccaaaggct acctctacgc
     3661 cgccatcggc ttctcgattt taatcgaatt gtttaaccag atttcgcagc gcaacagccg
     3721 caaaaacgac tacatcggca gctcgtggcg caagcgcacc gccgaaaacg tcttgggtat
     3781 gatgggtata cgcgaaagcg tgcttgccga cgcgggcggc gaatccgggg acgacgcgca
     3841 ttttgaagaa aacgaaaaat cgatgatacg cagcgtgctg acgcttgccg aacgcccgat
     3901 tatgggggtg atgatcccac gccgcgacat cgaacggctg gacatttccc aaagccgcga
     3961 agaacagtgt gcccaactgc aaaacacgcc ttacagccgc ctgctcgttg tcggtaaggc
     4021 gggcgtggac gaacctttgg gctacatcaa caaaaaagac ctgctgtccc aactgctgga
     4081 aacaggcggt ctcgacattc agacggcatt gcgccagccg ctcgtcctgc ccgacagcac
     4141 caccgcgctg ggcgcaatcg aactcttccg ccaaagcagc gcggattatg ctttggtggt
     4201 ggacgagttc ggcgcggtat tgggcatggt aaccatgaaa gacctgctcg aaaccatcgc
     4261 aggcgagttc cccgaagaat ttgagcgcga agaagaacca gccgttcagg ggaatcccga
     4321 tgaaagcctg acggtggaag gcgcgttgga atatgtggaa ctcgcaccgc aactcaacct
     4381 gccgcagcag gaggaagatg ccgatttcca tacggttgcc gggctgatta tggaagaatt
     4441 gcaaaccatc cccgatgtcg gcgattttgc cgatttccac ggctggcggt ttgaagtggt
     4501 cgaaaaagaa gggcagcgca tcgagcgggt caaaatcacc aaattgcccg aagaataagc
     4561 attcaggata gaaaatgaac gttttgattt ccaacgacga cggctacctc tccgaaggta
     4621 ttgccgtttt ggcgcgcgtt acggcggaat ttgccaacgt cagggtggtt gcgcccgaac
     4681 gcgacaggag cggggtcagc aattcgctga cgctggaacg ccctttgcag ttgaaacagg
     4741 cgcaaaacgg gttctactat gtcaacggca cgccgaccga ctgtatccac atcgggcagt
     4801 ctgtattttc ggattttcag gccgattttg tcttttcggg catcaaccgg ggcgcgaata
     4861 tgggggacga cacgctttat tcggggacgg ttgcggcggc aaccgaagcc taccttatgg
     4921 gcatacccgc tgtggcgttt tccttaaacg acgcttccgg acgctattgg gcgaccgcag
     4981 aacaggcact gtggacattg ttggcgcatt ttttcaaaaa ccccccgcag tcccctattt
     5041 tgtggaacat caatatcccc gccgtcgagc cggaagatgt gcggggcatt aaaatcgccc
     5101 gtttgggcag gaggcatcac ggtcagaacg tcatccccgc gcgcaatccg cgcggcgaac
     5161 agatttattg gataggcccg gtaggcaggg tttccgacaa agaagaggga acggatttcg
     5221 gtgaatgcgg cgcaggtttc attaccgtaa cgccgctgca aatcgacctg accgcctatc
     5281 cggacatggc ggaaacagcg gtgttctggc atgcggactg accgtttcat
//
LOCUS       DAWIVB010000149         5255 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWIVB010000149 DAWIVB010000000
VERSION     DAWIVB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.149"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            109..804
                     /gene="pgl"
                     /locus_tag="WIX60_001700"
     CDS             109..804
                     /gene="pgl"
                     /locus_tag="WIX60_001700"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ5305934.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            785..1771
                     /locus_tag="WIX60_001701"
     CDS             785..1771
                     /locus_tag="WIX60_001701"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ5305935.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1821..2669
                     /gene="hexR"
                     /locus_tag="WIX60_001702"
     CDS             1821..2669
                     /gene="hexR"
                     /locus_tag="WIX60_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ5305936.1"
                     /translation="MLSKISESLVNLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2790..4436
                     /gene="pgi"
                     /locus_tag="WIX60_001703"
     CDS             2790..4436
                     /gene="pgi"
                     /locus_tag="WIX60_001703"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ5305937.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEV
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4450..5001
                     /locus_tag="WIX60_001704"
     CDS             4450..5001
                     /locus_tag="WIX60_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ5305938.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKVKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         1334 a         1459 c         1390 g         1072 t
ORIGIN      
        1 cagtcccgat atttggtatc aaaagcataa acaactgttc ggccgacata ttgctcaacc
       61 cgtttccata acggaatatg ccgtctgaaa taaaaaaagg acacaaatat gtttgtttgg
      121 cacgaatacg aaaatgcggc agaagcggcg cagtctttgg ctgacgcagt ggcggatgct
      181 ttgcagggcg cactggacga gaagggcggt gcggtgttgg cagtttccgg cggacgttcg
      241 ccgattgcat ttttcaacgc cctgtcgcaa aaagatttgg attggaaaaa cgtcggcatc
      301 accttggcag atgaacgcat cgtgccgacc gtccacgccg acagcaatac cggtttggtg
      361 cgcgaatacc tgttgaagaa caaagcggaa gcggcaatgt ggattcctat ggtggaagac
      421 ggaaaaactg aaaccgaatt acatcccgat gctgttgtcg attatgcact gaaacattac
      481 aaacagcccg atgttttggt tttgggtatg ggaaacgacg ggcatacggc ttcgattttc
      541 ccgaaagctc cgcagtttca gacggcaatc gacggttcgg caggtgtcgc gttggtgcat
      601 accacgcccg ttaccgcgcc gcacgagcgc gtcagtatga ccttggatgc gattgcccat
      661 acggggcatg tgtttttggc gatacggggc gaagagaaaa aagccgtgtt cgaccaagcc
      721 gcacaaggcg aaaaccgcga atatccgatc aacctcgttt tgaaccatca aggagtgaac
      781 tgccatgtct tctacgccga ataaacaagc cggatatccc cgactggtcg ccgatatcgg
      841 cgggacgaat gcacgctttg cgctggaaac cgcgccgcgc gtcattgaaa aagccgccgt
      901 gcttccgtgt aaagactacg atacggttac cgatgcggtg cgtgcctatc tgaatcaaag
      961 cggtgcaaca gccgtacggc acgcggcatt tgccatcgcc aacccgattt tgggcgactg
     1021 ggtgcagatg accaaccacc attgggcgtt ttccatcgaa accacccgtc agactttggg
     1081 gctggacacc ctcatccttt tgaacgactt taccgcgcag gcattggcgg taacgcagac
     1141 ttcaagcaaa gacctgatgc aggtaggcgg gcaaaagcct gtcgaatttg cccccaaagc
     1201 cgttatcggc cccggtaccg gcctgggcgt gagcggattg gtgcacagcc acgcaggctg
     1261 ggtggctttg gcgggcgagg gcgggcatac cagtttcccg ccgtttgacg atatggaagt
     1321 gctgatttgg cagtacgcca aaaacaaata cggccatgtt tccgccgaac gctttttgag
     1381 cggcgcgggc ttgagcttgg tttacgaggc tttggctgca aaacagaaag ccaaacccgc
     1441 caaactgatg ccgtctgaaa tcacggaaaa ggctttgagc ggcgcgtcgc ctttgtgccg
     1501 tcagactttg gacatcttct gcgccatgct cggcacggtt gcttccaacc tcgccctgac
     1561 gctgggcgcg cgcggcggcg tgtacctgtg tggcggcatt attccccgcg tgttggaata
     1621 tttcaaaact tccccgttcc gcagccgttt cgagaacaag ggcaggtttg aagcatatct
     1681 tgccgcgatt cccgtgtatg tcgtcttgag cgagtttccc ggaatttccg gtgcggctgc
     1741 ggctctcgac aaccatttga gaaacgttta accacagcgg ctccttgcag cggggctgca
     1801 ttatcgaagg gcatatcatt atgttaagca aaatcagcga atcactggta aacctttccg
     1861 gtgcggaacg caaagtcgcc gaatgtgcat tggcggaacc caaatggttc gtccatgccg
     1921 ccgttgccga aattgccgaa cgtgcgtccg tcagccagcc gaccgtcatc cgattctgcc
     1981 gcagcttggg ttataaaggg ctgcccgagt tcaagctcgc cttgtccgcc agcatcggtc
     2041 atgagggtat gccctatgtc cacgaagaac tcaacgccga cgacgatatg gcaagcgtgg
     2101 tcgagaaagt gttgggcaat gccgccgcct cgctgttggg cgaacgccgc ttcctgaaag
     2161 agtcggagct ggaaaacgcc attgccacgc tgatgcacgc ccgtcgcgtc gagttttacg
     2221 gtgtcggcaa ttccggcatt gtggcacagg acgcgcagca taaatttttc cgtttcggca
     2281 tgtccaccgt cgcctatgtc gatacgcaca cgcagctgat ggcggcatct gttttgagcg
     2341 atcaggatgt tttggttgcc atttccaaca cgggttcgtc tatcgaactt ttggatgcgg
     2401 tcagcatcgc caaagaaaac ggcgcgtctg tcatcgcact gacccgcaac gattcgcctc
     2461 tggcgcaact tgccgactgc gtgttgagcg ttgccacaca ggaaaatgcc gaactctaca
     2521 cgcccatggt ttcccgcctc ttgcagcttg ccgtcatcga cattctcgcc atcggacttg
     2581 ccctgcgctt gggcgatgct gccagcctgc aattgcagaa aagcaaaaaa agcatacaca
     2641 acaagcacat cgattacgac aaagattgac cttcagacgg catcccacaa atgccgtctg
     2701 aaatgccgaa caacggtcgt cggcggcttg cggcagtttc cggcagcctt ttcatcccac
     2761 aacaaaaaac ctcattcagg agcatataga tgaaacacct tcacgactta cccgcatggt
     2821 cgaaattgtg gaatcacttt gacgacagca aaacattgca tatgcgcgaa atgttcgagc
     2881 aagacccgca gcgtgcggaa cgctactggc tgcaagtcgg cggactgacg ctggactact
     2941 ccaaaaaccg catcaacgac gaaaccatgt cgcttttgtt cgagcttgcc cgagaagcag
     3001 gcgtgccgga gcggatgcgg cagatgttcc acggcgaaaa aatcaatacc accgaaaacc
     3061 gcgccgtcct gcatgtcgcc cttcgcaacc gcaccaattc gccgattgtg gttgacggtg
     3121 aagatgtgat gcccaaagtc aaccgcgttt tgcaacgtat gggcgaattt gcacacgaag
     3181 tccgcagcgg aagctggctg ggctatacca accaagtcat taccgacgtt gtcaacatcg
     3241 gcatcggcgg atcggatttg ggtccgctga tgatgtgtac cgcgctcaaa cctttcggtc
     3301 atccgcgcct caatatgcac ttcgtctcca acgtggacgg ctcgcaactg cgcgacgtat
     3361 tgtccaaagt ccaccccgaa accacgttgt tcatcatcgc ctccaaaaca tttaccacgc
     3421 aggaaacgct gaccaacgcg ctgaccgcgc gcgaatggtt tttgaatcat gcgggcgacg
     3481 aagaagccgt tgccaaacac ttcgccgccg tttccaccaa tcaaaaagcc gtcgccgaat
     3541 tcggcatcga caccgccaat atgtttgaat tttgggattg ggtcggcggt cggtacagcc
     3601 tgtggtccgc catcggattg ccgattatgc tgtatctcgg cgaagaaaac ttcattgaaa
     3661 tgctcaacgg cgcgcacctg atggaccaac acttcatcaa cacaccgctc gagcgcaacc
     3721 tgcccgtcat tctcgccctc atcggcatct ggtatatcaa ctactacggc ggcggcagcc
     3781 acgtcatcgc gccttacgac caacatttgc accgcctgcc caaattcatc cagcagctcg
     3841 atatggaaag taacggcaaa caggttacgt tggacggcaa agcagtcgga cacgaaacct
     3901 cgccgattat ctggggcgaa acgggcatta acggccagca cgcctttttc caactgctgc
     3961 accaaggcac gcacattacc cccatcgacc tgattgcctc gcttgaaaaa cgcagcaacc
     4021 tgcccggaca ccacgaaatc ctgcttgcca acgtcttcgc ccaagcagaa gcctttatgc
     4081 gcggcaaaac ccccgacgaa gtccgcgccg aactcaaagc gcagggtatg gatgaggtgc
     4141 gcatcgaaga gctggtcccg cacaaaacct tctccggcaa ccgcccgacc aacctcattc
     4201 tcatggacaa ggtcaacccg cgcaatatgg gcagcctgat tgccatgtac gaacacaaaa
     4261 ccttcgtaca aggcatcatt tggggcatca acagcttcga ccagtggggc gtggaactcg
     4321 gcaaacaact ggctaaaacc attttgggcg aactgaccgg cgaaaccggg ccgcaaaagc
     4381 acgacagttc gaccgaacgc ctgatcaacc tctacctgca gaccaaccgc aaataaaacc
     4441 tgcggaaaaa tgccgtctga acgccgaccg tttcaaacgg catttttatc gaacaggaaa
     4501 accgtcggta aacttgcaga gcgtgtgcaa tcccgatatg atggtttgca taaatttaaa
     4561 catatatgtt ccgcagctat ggcactgatt aaagagccgt tggacaaagt gaaacaaagg
     4621 aacgaagaac ttgaagcggc agaagaagcg gcggcgcagg aggcattggg tcgggagcag
     4681 gaagccgccc gcgtatccga atgggaagaa cgctacaagc tgtcgcgcag cgagttcgag
     4741 cagttctgga aaggattgcc tcaaaccgta cagaataagc tgcaagcctc acagaaaaca
     4801 tggaaaagcg ggatggataa aatctgtgcc aacaatgcga aagctgaagg taaaacgcca
     4861 aacggcataa aattcagcga actggcatgc aaaacggcga aaaccgaagc acgcttggaa
     4921 gagctgcaca accgtaaaaa agcccttatc gacgaaatgg ccagggaagc ggacaagaaa
     4981 gaactgtcaa agcggctctg aacagcgcgg ttcaggcact gcccgccgat attgccgaaa
     5041 ccgtcatgcc cgagtaccgc aactgacaaa acggtttgaa tgccaaatgc gccgacggcg
     5101 acgaatacgg cgtggcacaa tctgactgcc gtaccagaga aatcaatgcg aaaaccaaag
     5161 aaatccaagg ttatctgatt gactgaaact tggatgcggg aatgtcggcg gcttttgcgt
     5221 ttttgtcgtt tttatagtga attaaattta aaccg
//
LOCUS       DAWIVB010000150         5233 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWIVB010000150 DAWIVB010000000
VERSION     DAWIVB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.150"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            86..1561
                     /locus_tag="WIX60_001705"
     CDS             86..1561
                     /locus_tag="WIX60_001705"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ5305939.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            1558..2103
                     /locus_tag="WIX60_001706"
     CDS             1558..2103
                     /locus_tag="WIX60_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ5305940.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            2100..3236
                     /locus_tag="WIX60_001707"
     CDS             2100..3236
                     /locus_tag="WIX60_001707"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ5305941.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLSTDSDTAFQEAKKLHQSL"
     gene            3283..3765
                     /locus_tag="WIX60_001708"
     CDS             3283..3765
                     /locus_tag="WIX60_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ5305942.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            3871..4926
                     /locus_tag="WIX60_001709"
     CDS             3871..4926
                     /locus_tag="WIX60_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5305943.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEKKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            4923..5216
                     /locus_tag="WIX60_001710"
     CDS             4923..5216
                     /locus_tag="WIX60_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305944.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITKHNGKPDTKPNTAAKM
                     PSEAVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQNQYFAVRIGTFIVD"
BASE COUNT         1497 a         1607 c         1106 g         1023 t
ORIGIN      
        1 cggttagact gtcaacaatt ccgataaaat gctatactcg cgcccattgc cgcgccgtcc
       61 gaaaccgccc tttgggaaag cagaaatgat cagcaaacaa gaataccaag cccaagccgc
      121 ccaaggctac aaccgcatcc cgctcgtgca agaactcctt gccgatttgg atacgccgct
      181 ttccctctat ctcaaactcg ccaaccgccc ctatacctac ctgctcgaat ccgttgtcgg
      241 tggcgaacgt ttcggccgct attcttttat cggcctgcct tgcagccact atctcaaagc
      301 cagcggcaaa cacgtcgatg tttatcaaaa cggcgaaatt gtcgagcaac acgacggcaa
      361 ccccctgccc tttatcgaag ccttccacaa ccgcttcaaa acacccgaaa tcccaagcct
      421 gccgcgcttt accggcggac tggtcggcta cttcggttac gaaaccatct acaattttga
      481 acacttcgcc caccgcctga aaaacaccac caaagccgac ccgctcggca cgcccgacat
      541 cttgctgatg ctgtcgcaag aattggcggt gatcgacaat ttgagcggca aaatccatct
      601 cgtcgtttac gccgatccgt cgcagcccga cggctacgaa cgcgcccgcg aacgcctcga
      661 agacatccgc acccagttgc gccaaagctg cgccatcccg ctctcgctcg gcagcaaaca
      721 caccgaagcc gtcagcgaat tcggcgaaga gccgttcaaa gcctgcgtta acaaaatcaa
      781 agactacatc ttcgcaggcg actgcatgca ggttgtcccc agccagcgca tgagcatgga
      841 attcaccgac agcccgctcg ccctctaccg cgccctgcgt acgctcaacc cttcgcctta
      901 tctcttttac tacgatttcg gcgatttcca catcgtcggc tcctcgcccg aaatcctcgt
      961 ccgccgcgaa cgcgacgatg tcatcgtccg ccccatcgcc ggcacgcgcc tgcgcggcaa
     1021 aacccccgcc gaagaccttg ccaacgaaca agatttgtta agcgatgcca aagaaatcgc
     1081 cgaacacgtc atgttgattg acttaggacg caacgacgtc ggccgcatca gtaaaaccgg
     1141 cgaagtcaaa gttaccgaca aaatggtgat tgaaaaatac tcccatgtaa tgcatatcgt
     1201 ttccaacgtc gaaggccgtc tgaaagacgg catgaccaac atggacatcc tcgccgctac
     1261 cttccctgcc ggcacactct ccggcgcacc caaagtccgc gccatggaaa tcatcgaaga
     1321 agtcgaaccg agcaaacgcg gcatctacgg cggtgccgtc ggcgtatggg gtttcaacaa
     1381 cgacatggat ttggcaatcg ccatccgcac cgccgtagta aaaaacaaca cgctatacgt
     1441 ccaaagcggc gcgggcgtgg ttgccgactc cgatccggcc tccgaatggc aggaaacaca
     1501 aaacaaagcc cgagccgtta tacgcgccgc gcagatggtg caggaagggt tggataaatg
     1561 acttatgaaa aactttatga gcttcgccaa acactcagac cgactaccaa agacggtctc
     1621 tatacagata atgaaaaaag tcgagaaatc ctaacggtta gatggagcgg caacacagaa
     1681 aatcctaaaa atttctcagc tgttgctata ggaattaatc caagtaaagc aaatgatgaa
     1741 agaagcgaca aaacccttac ccaacttgca cgtttcttgg atatgtacgg ttttaccaac
     1801 ttcaaaatgt taaatatttt ttccagttac tctacacagc aaacaggaat tagggcaaac
     1861 acccaaaccg atttttccaa atttaaagga tgtttggaag atgctgatat gattattctt
     1921 gcatggggta cagaccgcag tgcttataag gatgaaaaaa acagaatact ggaatttcta
     1981 aaagcagaaa aatttatgga aaaagtattc tgtatttctg aaactggtaa ttccagcgat
     2041 acaagacatc cgtcaagaat tagttatagc tatcaacttg tacaatttga agagtcagca
     2101 tgaaaaacat atctctttct ccgcccgcca tgctcggcat tcttggcggc ggacaattag
     2161 gcagaatgtt tactgttgct gccaaaacca tgggctacaa agtaaccgta ctcgatccca
     2221 acccgaatgc gccggcagcg gaatttgccg accgccattt gtgtgcgccg tttgacaacc
     2281 aaaccgcttt ggaagaattg gcaaaatgtg cggctgttac gaccgagttc gaaaacgtca
     2341 atgccgatgc gatgcgtttt ctcgccaaac ataccaatgt ttcccccagc ggcgactgcg
     2401 ttgccatcgc gcaaaaccgc attcaggaaa aggcatggat acgcaaagca ggcctgcaaa
     2461 ccgcgccgta tcaagcaatt tgcaaagccg aagacatcac tgaagaaagc atacaatttc
     2521 tgcccggcat cctgaaaacc gctacattgg gctatgacgg caaaggccaa atccgcgtca
     2581 aaacggtgga tgaactcaaa gccgcgtttg ccgaacaccg cggcgtggat tgcgttttgg
     2641 aaaaaatggt ggacttgcgc ggcgaaattt ccgttatcgt atgccgtctg aacaatgaca
     2701 acgtgcaaac tttcgatcct gccgaaaaca ttcacgaaaa cggtatcctc gcctactcca
     2761 tcgtcccagc ccgactgagt gccgacattc agcaacaggc gcgacaaatg gcgcagcgtt
     2821 tggccgatga attgaactac gtcggcgtat tggcggtaga aatgtttgtt gtcggcgaca
     2881 cgcatgaatt ggtcgtcaac gaaatcgcgc cgcgtccgca caattccggc caccataccg
     2941 tcgacgcctg cgcggcagac caattccagc aacaggtccg cctgatgtgc aacctgccac
     3001 ctgctgacac caaattgctg agttcttgct gtatggcgaa tattttgggc gacgtttggc
     3061 aggaagacgg cggcgaaccg gattggtttc ccctgcaaag ccggccggac gcgcacctgc
     3121 acctttacgg caaaaaaacc gcgcacaaag ggcggaaaat gggacacttt accgttttaa
     3181 gcaccgattc ggacaccgca tttcaagaag caaaaaaact gcatcagtcc ctataatccg
     3241 accaacggca cagatgccgt ctgaagccgc cgacatactg ccatgaacct gctgacactg
     3301 ttacgccccg ccaccgtgca agactgcaaa gacatcttca aagtccacct gcactccgtc
     3361 caatacacct gcatcctcag ctacaacgaa cacgccctaa aagtatggga aggcttgtta
     3421 aacacagaaa gctacctgcc gaccatttcc gatcccgaca aagcactttg ggttgccgaa
     3481 tacaaaggca acatacaggg atttttccaa atcgactgcc aagaagcaca actcgatgca
     3541 ctttacgtcc accccctgtt ccacaacctc ggactgggca ctgccctgct tcatcaggcg
     3601 gaaaccatcg cccacaaatc cggtttgagc tttctgaaac tctacgcatc gctcaactcc
     3661 gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt cggcagtatt gcagcttgac
     3721 cccagcatca aaatcaaatg cgaactgatg cggaaacacc tctgatccgt ccttataacc
     3781 aaaactgata atcttcaaat caaacattat ttccacaagc gcagacagcc gtataaagtt
     3841 gccccatcta ccaaccgaca ggacaagcat atgaaaacct acgcaccggc actctatacc
     3901 gcagccctgc tcaccgcctg cagccccgca gccgattcaa accatccgtc cggacaaaat
     3961 gccccggcca ataccgaatc cgacggaaaa aacattaccc tgctcaacgc ctcatacgat
     4021 gtggcacggg atttttacaa agaatacaac cccttattta tcaaaacata ccaatccgaa
     4081 caccccggca catccgtcag catccaacag tcccacggcg gctccagcaa acaggcatta
     4141 tccgtagcca acggccttca agccgatgtc gtaaccatga accaatcctc cgacatcgac
     4201 ctgctcgaaa aaaaaggact ggtagaaaaa ggctggcaac aagccctccc cgaccacgcc
     4261 gcgccctaca ccagcactat ggttttcctt gtccgaaaaa acaaccccaa acagatccgc
     4321 gattggaacg accttgccaa agacggcgtt aacatcgtca tcgccaatcc caaaacctcg
     4381 ggcaacggac gctacgcctt cctcggcgca tacggttacg gtctgaaaac caccaacggc
     4441 aacgaacagg aagcccaaaa actcgtcgca tccatcctca aaaacacccc cgtttttgaa
     4501 aacggcggac gcgccgccac caccaccttc acacaacgca acatcggcga cgtactcatc
     4561 acttttgaaa acgaagccaa ctacgtcagc aaaaaactga cccaaggaca gtttgaaatc
     4621 gtctatccga gctacaccat ttccgccgaa agccccgtcg ccgtcgtcaa cagcgtcgtc
     4681 gcgaaaaaag gcacgcaaaa aaccgcccgc gcctatctcg aatacctttg gagcgaaccg
     4741 gcgcaagaac tcgccgcctc actctacctg cgcccccgca accccgaagt attggcaaga
     4801 cacaaagccg acttcccaga cttagacacc ttctctcccg aaaaaaaatt cggcggatgg
     4861 gacaacatca tgaaaaccta cttcgccgac ggcggcatat tcgaccgcct gaccgcacaa
     4921 aaatgaccgc tatgccgtct gaaccgcctt tcagacggca tttcacacat caaaacagaa
     4981 aaaccgcgcc caaaatcatc atcacaaaac acaacggcaa accggataca aaacccaaca
     5041 ccgccgccaa aatgccgtct gaagccgtcg gcgcaaccat ccttatctgg ggcccgcttc
     5101 aggggcgcat aactttcctt aaccatcctt ttgacagcca aataaacagt gcttttgcct
     5161 gtcagaaaca aaaccaatat tttgcagttc gtatcggaac atttatagtg gattaacaaa
     5221 aaccagtaca gcg
//
LOCUS       DAWIVB010000151         5224 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWIVB010000151 DAWIVB010000000
VERSION     DAWIVB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.151"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            133..2295
                     /locus_tag="WIX60_001711"
     CDS             133..2295
                     /locus_tag="WIX60_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ5305945.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGSSVETTPVNRPAVGAAMRLLRRNTAFHREDGTAIPDSKQAEEKLSFKEG
                     DVLFLYGSKENKLQQLKSEIHKRNPEASITTSENENKKYNYRFVSAGYVFTKNGKDEI
                     EKTSDEKQFSNRLGYDGFVYYSGEHPSQSLPSAGTVKYSGNWQYMTDAKRHRTGQAVS
                     SVDLGYTTYYGNEIGAASYEARDADSREKHPAEYTVDFDKKTLEGKLIKNQYVKPNEQ
                     KKPLTIYDITATLDGNRFTGSASVNPDLATNHANKEHLFFHANADQRLEGGFFGDNGE
                     ELAGRFISNDNSVFGVFAGKQKTDASGTNPAMPSEKHTKILDSLKISVDEASDKNPRP
                     FAISPMPDFGHPDKLLVEGREIPLVSQEKTIDLADGRKMTVRACCDFLTYVKFGRIKT
                     DRPASKPKAEDKGKDEEDTGVDNGEESEDEIGDEGGGAEDEAAENEGGEEDEAEEPEE
                     ESPAEGGSGSDGISPAPEAPKGRDIDLFLKGIRTAETNIPQTGKARYTGTWEARIGKP
                     IQWDNHADKAAKAEFDVDFGEKSISGTLTEKNGVEPAFHIEKGVIDGNGFHATARTRD
                     NGINLSGNGSTNPQSFKANNLLVTGGFYGPQAEELGGTIFNKDGKSLDITEDTEHEAE
                     AEVENEAEAGVGEQLKPEVKPQFGVVFGAKKDNKEVEK"
     gene            2292..5126
                     /gene="lbpA"
                     /locus_tag="WIX60_001712"
                     /gene_synonym="tbpA"
     CDS             2292..5126
                     /gene="lbpA"
                     /locus_tag="WIX60_001712"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ5305946.1"
                     /translation="MNKKHSFPLTLTALAIATAFPAYAAQAGGATPDAAQTQSLKEVT
                     VRAAKVGRRSKEVTGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIQNPNKKAEYFLAEGEREP
                     KPVAKLAGNGNYLKNQLNRWVKERIEQNQPLSAEEEAMVREAQARHENLSAQSYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAKKF
                     SDKSGVYDGDDFRDGLYFRPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISEHT
                     GFDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
BASE COUNT         1492 a         1219 c         1388 g         1125 t
ORIGIN      
        1 atgtatctgt actgtctgcg gctcgtcgcc ttgtccggat ttttgttaat tcactataaa
       61 aacgggttga tattatctgt acatattaat ataatgataa ttattattaa tcaaatagga
      121 ggaaaagtag ggatgtgtaa accgaattat ggcggcattg tcttgttgcc cttacttttg
      181 gcatcttgca tcggcggcaa tttcggcgtg cagcctgttg tcgaatcaac gccgaccgcg
      241 taccccgtca ctttcaagtc taaggacgtt cccacttcgc cccctgccgg gtcttcggta
      301 gaaaccacgc cggtcaaccg gcccgccgtc ggtgcggcaa tgcggctgtt gagacggaat
      361 actgcttttc atcgtgaaga tggcacggca attcccgata gcaaacaagc agaagaaaag
      421 ctgtcgttta aagaaggtga tgttctgttt ttatacggtt caaaagaaaa taaacttcaa
      481 caacttaaaa gcgaaattca taaacgtaat cctgaggcaa gcattaccac atcggaaaat
      541 gaaaataaaa aatataatta tcggtttgtc agtgccggtt atgtgtttac taaaaacgga
      601 aaagatgaaa ttgagaaaac atcggatgaa aagcagtttt ctaatcgttt aggctatgac
      661 ggttttgtat attattccgg agaacatcct tcccaatctt tgccgagtgc gggaacggta
      721 aaatattccg gtaactggca atatatgacc gatgccaaac gtcatcggac aggtcaggcg
      781 gtttccagtg tggatttggg ttataccaca tattatggta atgaaattgg ggcagcttct
      841 tatgaggcta gggatgccga tagccgggaa aagcatcctg ccgaatatac ggttgatttc
      901 gacaaaaaaa ccctagaggg caagttgatt aaaaaccaat atgtcaaacc caatgagcag
      961 aaaaaaccac tgaccattta cgacattacc gcaacattgg acggcaaccg ctttaccggc
     1021 agtgccagtg tcaatcctga tttagcgaca aaccatgcca ataaggagca tttgtttttc
     1081 catgctaatg ccgatcagcg gcttgagggc ggttttttcg gcgataacgg agaagagctt
     1141 gccggacggt ttatcagcaa cgacaacagc gtattcggcg tattcgcagg caaacaaaaa
     1201 acagacgcat caggtacaaa tcctgctatg ccgtctgaaa aacacaccaa aatcttggat
     1261 tctctaaaaa tttccgttga cgaggcaagt gataaaaatc cccgcccgtt tgccatttcc
     1321 cctatgcccg attttggtca tcccgacaaa cttcttgtcg aagggcgtga aattcctttg
     1381 gttagccaag agaaaaccat cgatcttgcc gacggcagga aaatgaccgt ccgtgcttgt
     1441 tgcgattttc tgacctatgt gaaattcgga cggataaaaa ccgaccgccc ggcaagtaaa
     1501 ccaaaggcgg aagataaagg gaaggatgaa gaggatacag gcgttgataa cggcgaagaa
     1561 agcgaagacg aaatcggcga tgaaggagga ggtgcggaag acgaagccgc agaaaacgaa
     1621 ggcggcgaag aagacgaagc tgaagaaccc gaagaagaat cgccggcaga aggcggcagc
     1681 ggttcagacg gcatctcgcc cgctccggaa gcccctaaag gcagggacat cgaccttttc
     1741 ctgaaaggta tccgcacggc agaaacgaat attccgcaaa ctggaaaagc acgctatacc
     1801 ggcacttggg aagcgcgtat cggcaaaccc attcaatggg acaatcatgc ggataaagcg
     1861 gcaaaagcag aatttgacgt tgatttcggc gagaaatcga tttccggaac gctgacggag
     1921 aaaaacggtg tagaacctgc tttccatatt gaaaaaggtg tgattgatgg caacggtttc
     1981 cacgcgacag cgcgcactcg ggataacggc atcaatcttt cgggaaatgg ttcgactaat
     2041 cctcaaagtt tcaaagccaa taatcttctt gtaacgggcg gcttttacgg cccgcaggcg
     2101 gaggaattgg gcggtactat tttcaataag gatgggaaat ctcttgatat aactgaagat
     2161 actgaacatg aagctgaagc tgaagttgaa aatgaagctg aagctggtgt tggcgaacag
     2221 ttaaaacctg aagttaaacc ccaattcggc gtggtattcg gtgcgaagaa agataataaa
     2281 gaggtggaaa aatgaataag aaacacagtt ttccgcttac cctgactgcc ttggccattg
     2341 caaccgcttt tccggcttat gctgctcaag cggggggggc gacacctgat gccgcgcaga
     2401 cccaatccct gaaagaggtt accgtccgtg ccgccaaagt gggacggcga tcgaaagaag
     2461 taaccggttt gggcaaaatc gtcaaaacgt cggaaacgtt gaacaaagaa caggtactcg
     2521 gtatccgcga cctgacgcgc tatgatccgg gcgtggcggt tgtcgaacag ggcaacggcg
     2581 cgagcggcgg ctactcgata cgcggcgtgg ataaaaaccg tgtggcggtt tcggttgacg
     2641 gcgttgccca aatacaggcg tttaccgtgc agggatcgtt gagcggatac ggcggacgcg
     2701 gtggcagcgg tgcaatcaac gaaatcgaat atgaaaacat cagcacggtg gaaatcgaca
     2761 aaggtgccgg ttcgtccgat cacggcagcg gcgcgctcgg cggcgcggtc gccttccgca
     2821 ccaaagaggc ggcagacctg atttcagacg gcaaaagttg ggggatacag gcaaaaactg
     2881 cctacggcag taaaaaccgc caatttatga agtcgctcgg cgcggggttc agcaaagacg
     2941 gttgggaagg gctgctaatc cgaaccgaac gccaagggcg ggaaacgcgc ccgcacggcg
     3001 atattgcaga cggggtggaa tacggcatag accgtttgga cgcgttccgc cagacatacg
     3061 atattcagaa tccgaataaa aaggcggaat atttcttagc ggagggcgag cgtgaaccca
     3121 agcctgtggc aaaattggcg ggcaacggaa actatttgaa aaaccagctc aaccgctggg
     3181 taaaagaacg tattgaacaa aatcagcctt taagtgccga agaagaagcg atggtgcggg
     3241 aggcgcaggc gcgccacgaa aacctgtccg cccaatcgta cacgggcggc ggcaggatat
     3301 tgcccgatcc gatggattac cgcagcggct cttggcttgc caagctgggc taccgcttcg
     3361 gcggcaggca ttatgtcggc ggcgtgtttg aggataccaa acagcgttac gacatccgcg
     3421 atatgacgga aaaacagtat tacggtacgg atgaggcgaa aaagtttagc gacaagagcg
     3481 gggtgtacga cggcgacgat ttccgcgacg gtttgtattt taggccgaat atagaagagt
     3541 ggaagggcga taaaaatttg gtcaagggca taggtttgaa atattcccgc accaaattta
     3601 ttgacgaaca tcaccgccgc cgccgtatgg gtttgctgta ccgttatgaa aacgaagcgt
     3661 attctgacaa ttgggcggat aaggcggtgt tgtcgtttga caaacagggc gtggcaaccg
     3721 acaacaacac gctgaagctg aattgcgccg tgtatcctgc cgtggacaaa tcttgccgcg
     3781 cgtcggcaga caagccgtat tcctacgaca gcagcgaccg tttccactac cgcgaacagc
     3841 acaatgtttt gaatgcctcg tttgagaagt cgctgaaaaa caaatggacg aaacaccatc
     3901 tgactttggg cttcggttac gatgcttcca aagcgatctc ccgccccgaa cagctttccc
     3961 acaatgcggc aaggatttcg gaacacacgg gattcgatga aaagaatcaa gataagtacc
     4021 gtttgggtaa gcccgaagtc gtcgaagggt cggtctgcgg ctacatcgaa accctgcgtt
     4081 cccgcaaatg cgtgccaaga aaaatcaacg gcagcaatat ccacatttct ttgaacgacc
     4141 gtttttcaat cggcaaatat tttgatttca gcttgggtgg caggtacgac cggaaaaact
     4201 tcaccacgtc ggaagaactc gtccgcagcg gacggtatgt tgaccgttcg tggaacagcg
     4261 gcatcgtgtt caaaccgaac cggcattttt ctttgtctta ccgcgcctcc agcggcttca
     4321 gaacgccctc attccaagaa cttttcggga tagacattta tcacgattat ccgaaaggct
     4381 ggcagcgtcc cgccctgaaa tcggaaaagg cagccaaccg cgaaatcggt ttgcagtgga
     4441 agggcgattt cggctttttg gaaatcagca gcttccgcaa ccgttatacc gatatgattg
     4501 ccgttgccga tcacaaaacc aaattgccga atcaggcagg acaattgaca gagattgata
     4561 tacgcgatta ttacaatgcc caaaatatgt cgctccaagg cgttaatata ttgggaaaaa
     4621 tcgactggaa cggtgtgtac ggcaaactgc ccgaaggttt gtacaccaca ttggcataca
     4681 accgcatcaa accgaaatcg gtatccaacc ggccggactt gtccctccgc agctatgctt
     4741 tggatgcggt acagccgtcg cgttatgttt tggggttcgg atacgaccag ccagagggga
     4801 aatggggcgc aaacattatg ctgacctatt ccaaagggaa aaaccctgac gagcttgctt
     4861 atctggcagg cgatcaaaaa cgatattcga caaaaagagc gtcgtcttct tggtcgacgg
     4921 cagacgtttc cgcctatctg aatctgaaaa aacggctgac cttgagggcg gctatctaca
     4981 atatcggcaa ctaccgctac gttacttggg aatccttgcg ccagactgcg gaaagcacgg
     5041 caaaccggca cggcggcgac agcaactatg gaaggtatgc cgcaccgggc aggaacttca
     5101 gcctcgcgct cgaaatgaag ttttaaagga aataccgtct gaaagcttga tctgcacccc
     5161 aaaagtcgga ctaaaccgcc aactgattaa ggtgcaggtt tttttgattc gatataaaca
     5221 agat
//
LOCUS       DAWIVB010000152         5163 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWIVB010000152 DAWIVB010000000
VERSION     DAWIVB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.152"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(72..1328)
                     /locus_tag="WIX60_001713"
     CDS             complement(72..1328)
                     /locus_tag="WIX60_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ5305947.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGSQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETAFLG
                     NLKARKTEFSDSLNTAAELPQWLVEQLKQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAIGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSVLPEENELQVERFLSEHPEFELVNCAELLQSLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(1676..2017)
                     /locus_tag="WIX60_001714"
     CDS             complement(1676..2017)
                     /locus_tag="WIX60_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305948.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2221..3078)
                     /locus_tag="WIX60_001715"
     CDS             complement(2221..3078)
                     /locus_tag="WIX60_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ5305949.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELSVRELLGE
                     HGFRYLLALDADPTRLTDYLAQRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVARLNGKPYHIELTCKYYGGDTDSPEGMRGFDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLNVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3139..3954)
                     /locus_tag="WIX60_001716"
     CDS             complement(3139..3954)
                     /locus_tag="WIX60_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ5305950.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4198..4983
                     /locus_tag="WIX60_001717"
     CDS             4198..4983
                     /locus_tag="WIX60_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ5305951.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT         1169 a         1194 c         1409 g         1391 t
ORIGIN      
        1 tttgcatttt gaaaatgaga ttgagcataa aattttagta acctatgtta ttgcaaaggt
       61 ctcaaaccgg tttatttgcg ttgcaatacg gcggcgaaga agccgtcggt ttggtgtcgg
      121 gcggaatcga ggcgcaggta ttttccggta tccaaatcaa ccttcaggct ttgcagcagt
      181 tcggcgcagt tgacgagttc aaattcggga tgttcggaca ggaaacgttc gacttgcagt
      241 tcgttttctt ccggcaacac gctgcaggtg gcgtacacca agcggccttg cggtttcacc
      301 agtttggatg cggcatcgag gatgctgtgt tgctgttcca aaaggttggc cacggtctcg
      361 gcggattggc ggtatttgag gtcgggattg cggcgcagcg tgcctaagcc ggagcagggc
      421 gcatccacta aaacgcggtc ggctttgcct gccagtcggg cgatacgggt atcgtgttcg
      481 ctgctgatgc gttcggggtg gatgttggtc agtccggcgc gggtcatacg cggtttgagg
      541 ttggcaaggc gtttttcggc gatgtcgaag gcgtagattc tgcctttgtt cgccatttgc
      601 gcgccgatgg cgagggtttt gccgcctgcg ccggcacaga aatcgacaat gatttcgccg
      661 cgttttgcgc ccaccaataa agcaagcagc tggctgcctt cgtcttggac ttccagtgtg
      721 ccgtccaaaa acagctcgtg tttgttaagc gcgattttgt ttttcaggcg gatgccccaa
      781 ggcgaataag gcgttgcctc tgcatcggca ctttcggctt gcaacagcgg cagcacttta
      841 tcgcgtttgc ctttcaaagt gttgacgcgg atgtcgagcg gggcaggctg gttgatgctg
      901 cggccgaaag cgaggatttc ttcttcgcgc caatgctgtt tcagttgttc caccagccat
      961 tgcggcaatt ctgcggcggt attcaggctg tctgaaaact cggttttacg ggctttcaaa
     1021 ttgccgagga acgctgtttc ttcttcatca agcaggtctt tgatttggct gatgttggtg
     1081 cttctgccga gaaccagtgc ggcgagagcg gctttgcgcg gctgcgcgtg cggacggcgt
     1141 agggcggtac tgattttttg atagtggcgc agcgcggcaa aggcggtttc ggcgatttcg
     1201 tggcgatctt gactgccgag ctttttgtgt tcgcggaaat aggcggagag gacggcatcg
     1261 gcaggctgtt tgaaagtcag catttcagcc aaaactttgg cggtatggtc gagttgtgcg
     1321 gcgttcataa tgtaatgggt tctcttactg tgggttattc ataatcaatc taacgtcctt
     1381 aaaataacaa tagcatcttt atcattactt agtattctat ccgcaataag aaagatgtgt
     1441 taaaagtgcg caatcgggat ttgtctaaat ttctccgtta actacaaagg cagcctgaaa
     1501 aaagttctat tttcaggtcg cttgaacagc atccgatata atcacatcag cgatttcatt
     1561 ttgtaatcac tccctaaaac atgaggaaaa aaacaaaatt tattttcttc gttcagtata
     1621 tctatcctaa atatgccatc actccattga gtttttaatt tgtatttcgc tcttgttaaa
     1681 tgtttttctg tttgccgatt tcttttgcta atgtaaaaaa tactctacca ctctcattgg
     1741 ttaaagtttt caagtattca tcaacatcag taacattaat aacaactgtt tcatttgtat
     1801 gtttattaaa ttctaaatca ataataatat tcccattaac caatatttca tatatatctt
     1861 cttcttcagg gagcatagac aaacaataag cacttgaagc tgaaaactct tttttcagat
     1921 actgaagaag aaaagtattt ttaattaaaa tattattaaa tttatctaat gattgcttga
     1981 cttctttagt tgtccaattt ttaacattat tcttcattgt attctacctg gggtcgtagt
     2041 tattaaatta ccatacttat ttgtttggat cgtcataata ttagttggca tgttgttgtt
     2101 atatttatca tatccaatat ccttattaac tcggatgact ctttcataaa ttgtatggga
     2161 aatgagattt ccatcactaa gcctctcaat agatatacgc gttaatgttg ggaaagggtt
     2221 tcagacggcc ttcataatgc gttcgatttc gtgccaaaag ccgtccggcc gacattccaa
     2281 aacggcaatc aagccttggt cgatacggcg gatttcggtt gcgttcaatg tttcggtttc
     2341 ggcgacacgc gcaggcgcga ggtaggccat acggtcgagc agatggtagc ggacttcttg
     2401 gcgtttaaac ccgtattccg cccaatcttg aatatagatg ccgcgccaac cgtatggatt
     2461 aagcggtggc tcaaaagcat ggaaaccgtg tggaaaaaat ccgatgccgc gcacgatgga
     2521 aacgggtttt acgttaagcg gcaaaccgtg ctgccgcaag gtccggatgc cgtctgaagt
     2581 gtgcggcaga ccgagttggg cggtcagttt ggcggctttt cccaacagcg tgtctttggg
     2641 gtcgaatccg cgcatccctt cgggactgtc cgtgtcgccg ccgtaatatt tgcaggtcag
     2701 ctcgatatgg tagggtttgc cgttaagcct tgccacaaaa tccgccgcgc cttgcgtatt
     2761 gccgtccgaa ccggaaaccg tgaggttgtg cgcgagcagt tcggcgtgcg gtgcattggc
     2821 aaaccaaaaa gccagcagct cttcggcata aatgccgaga cggtggccga acggggcgcg
     2881 ttgggcgagg taatccgtca gccgcgtggg atcggcatcc aatgccaaaa ggtaacggaa
     2941 accgtgttct cccagtagtt ctcgcacgct caattcgcag ccgctttgcc acaaaggcgg
     3001 cgcagtcagc agcgaggcaa ggtcgcggac gggttggctg gtaagtttcc accataatgc
     3061 gtctagggcg taattcatgc ttatccgaat atcagtcatg ccgtctgaaa agctggaaat
     3121 gttttcagac ggccttaatc agtctttttt atggatacgg tggctgacgg tattgaaaaa
     3181 accgcgcagg atatcgatgt cttcggtttg cgtattggcg cggccgaaca ggctctgcat
     3241 acggcgcatc agacgctcgc cgttgcggcg gttgaaaaag ccgatgtcgt tcatcacgct
     3301 ttccatgtgg gcgaccatgc ctttgatttg ctcgtgggtc gcagcgtggt cttcttgttg
     3361 aagatgggtc atgggcgaac cggtttggct gaagatttcg tagcacacga cctgcacggc
     3421 ttgggcgagg ttgagcgaga aatagtcggg attgccgttg atggtcatca gtcggttgca
     3481 ggcttggact tcttcgatgc tcaagccgaa agtctcgttg ccgaaaacca gtgccacttt
     3541 ctcgcctcgg tttgcggtct gcagtaattc ggataccaaa tcgcgcgggg tttgcagcgg
     3601 cgcagtaatt tcgcggcggc ggctggtcag ggcgcaggcg atggtggtgt cggcaagggc
     3661 ttcgtccaaa gaagcggcaa tggtggcatt ttccaaaacg tctgccgcgc cggaagcgag
     3721 gatgaagctt tcttccggta atttaaacga ttgaggatgc tccgggtcaa acacgggcgg
     3781 gttttccgtc atcggcgttg ccatcagatt tggggcgacg atggtcagtt tgtgcagacc
     3841 cattgttttc atcgcgcgcg cggcagagcc gatgttggcg ggatggctgg tgcgcgtgag
     3901 gatgatgcgg atgttgtcca gataagcggg cagggcgggt ttgagagtag tcatgttcgg
     3961 ttttcggtcg gaatggaata gggtataatg cgccgtcttt ttcgttcgga cggtcaaatg
     4021 ccgtctgaaa tgttctttaa caacgtcgga aatttaacgt tgcgcctgaa gcagacacgc
     4081 gtcaggcggt attttacccg atttggcagg atttacccca accaaataaa ctccgatttt
     4141 gccgctccga aggactgtac gtccgaaccg gcggtatgcc acccttaagg aaatctgatg
     4201 aatccgtttt tgaatacagc ctttaaagcc gcccgccgtg ccggtcagat gatgatacgc
     4261 gccgcgggca atctcgatgc cgtcaaaacc gacagcaaag ccttcaacga ttttgtttcc
     4321 gatgttgacc gcaattccga aatcatcctg gttgaggctt tgaaagaagc ctatccgcac
     4381 cacaaaatca cttgtgaaga aagcggctcc cacggcaaag ccgcagccga gtacgaatgg
     4441 attatcgatc cgctcgacgg cacgaccaat ttccttcacg gtcatcccca atacgccatc
     4501 tctatggcgc tgctgcacaa aggcgtgttg caagaagctt tggtttacgc ccccgaacgc
     4561 aacgacgtat atatggcttc gcgtggcaaa ggcgcgttgc tcaacgaccg ccgcatccgc
     4621 gtttccaacc gcatcgaact gaaccgctgc ctgattggta ccggcttccc tgttgtcgat
     4681 caaagcatga tggacaagta tctggcgatt ttgaaagatt tcttggcaaa aaccgccggc
     4741 ggccgtcgtg aaggtgcggc ttctttggat ttgtgcgccg tagcaacagg acgttttgac
     4801 ggctttttcg agtttaacct caaaccgtgg gacattgccg ccggtgcatt gattgttcag
     4861 gaagcgggcg gtatcgtaac cgatatgtcg ggtgaagacg gctggctgga aagcggcgat
     4921 attgtggctg ccaatcccaa agtgctggcg caaatgttga aaatcatttc cgcacacgtt
     4981 taaatttcag cccgctcccg ggaatgcggg aaaacgggcg ggtatgccgt ctgaagtatc
     5041 gcgccgatat ttcagacggc atttttactg gcagcggggg caggaaggtg gaaacggcta
     5101 aatgcccgaa actgccgaag atggaaaccc atatctgatt aaacggagaa ccctgtacga
     5161 ttt
//
LOCUS       DAWIVB010000153         5138 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWIVB010000153 DAWIVB010000000
VERSION     DAWIVB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.153"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(366..2642)
                     /locus_tag="WIX60_001718"
     CDS             complement(366..2642)
                     /locus_tag="WIX60_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ5305952.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            3064..3654
                     /locus_tag="WIX60_001719"
     CDS             3064..3654
                     /locus_tag="WIX60_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ5305953.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3718..4776
                     /gene="trpD"
                     /locus_tag="WIX60_001720"
     CDS             3718..4776
                     /gene="trpD"
                     /locus_tag="WIX60_001720"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ5305954.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4928..>5138)
                     /locus_tag="WIX60_001721"
     CDS             complement(4928..>5138)
                     /locus_tag="WIX60_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ5305955.1"
                     /translation="YTVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFS
                     HLRKFNGIPKEHLGLHLKKCQWHFK"
BASE COUNT         1185 a         1181 c         1451 g         1321 t
ORIGIN      
        1 cgcctgcgcg ggaatgacga cccattagtt acctaaaatt taaaaaacag aaacctttcc
       61 gcgtcattcc catgaaagtg ggaatctaga acccaaatgc taaggcgatt tatcggaaac
      121 ggctgaaacc gaacgaattg gattcccgcc tgcgcgggaa tgacgggatc ttgggtttct
      181 gcttttgatt tttctgcttt tgcgagaatg acggcgtgaa agtaagaatg atgaaacaaa
      241 aaaaatggga atgatggcat agtggtttgt tctttgtctt tgccatattt cctaacaaat
      301 tgattaaaaa gaaaaaaggt tttcagaatg ccgtctgaaa accttttttg tttgcctgtc
      361 cgattttaaa acttcacgtt cacgccgccg gtaaagctgc ggcccatttg cggcgtatca
      421 gagagaaagc tgctgtgggc gtaaacggat tggttgagca ggttgtcggc tttgacgtac
      481 caattccact cgccatagcg cgtattgcgg cggtagtttg cgccgaggtt gagcatatgg
      541 tgtccgggcg tgcgcgtttc gtagcgggcg agtttgtttt gggcgaacac gcggtagtag
      601 tccaaattgg catcgatacg gtcggtcagc gaggctttca ggtggaagcc gaggcgcgca
      661 gccggaacac ggggggcatt ttggtcgtcc tgtgcgatga aaggacggtt gccgtaggca
      721 tcttctctgc cgggtaggga aggcaggttt ttcagacggc ctcgtacata gtcgccggaa
      781 acgccgatgc ggtagcgcgg tgtcggtttg aagtagattt cgccttccgc gccgtagaag
      841 tcggcgccgg attggttgta gcgcacgagc ttcatttcgc tgtcgtcttc gatggatttg
      901 gggccgcgtc cgtcgtttaa ggtttgggcg taaatgtagt taccgaagcg gttgcggtag
      961 agtgccagat tgtattgcca gcggtcgcct tcgtagccca gcgcgagttc gatattgttg
     1021 gaacgctctt tgttgaggtg tttgttgccg acttcaaagg tgttggtggc gacgtgtttg
     1081 ccgtgtgcgt acagctcttg cgttgacggc aggcgttcct gatgggaggc ggtcaggctg
     1141 agtttgtgtt gtggcgtgaa ataccagttg cccgaaagtg cgaatgagcg ggcggtttgg
     1201 cggtgcgcgc cgaggtcggg cagggggtgg ttgtagtagt tttcccgatc aatcaatgct
     1261 ttgtcgtact gaatggaggc tttttgtttt tccacgcgta cgccgccttc aagcgtgaag
     1321 ttgtcccagt ttgcctgttc tacaccgaaa aagctgtaat gttgcacttt gttgtcaagc
     1381 agcatcggtt gtttaaccgc ttcggatatg gcagataaag cactggattt ttgttgtaaa
     1441 tattgcacgc cccagctgcc tttcagacga cctatgggtt ggtggcgcaa ctcgatgcgg
     1501 gcgttttgcg tttggttgtt aaaaaagttt tcgactgcat cgcctgcttt ttcgtcgtgg
     1561 cggtagtcgt tgcggttcag gtgtacgcgc agggcttcaa aaccggggaa cggttgcttc
     1621 cattcggcac ggagttcgta gcgtttgttg cgcaggtcta tccacggtct gccgctgtgg
     1681 gtgtgtgcgt gtgcattatc gtcgtcgtgg aagccgcagc tcaagcccgg attgtcgtaa
     1741 tcgatgtctt cttcggtcaa caggtgcgga taaagctgta aatagcgttt gttaatcaag
     1801 ctcttttgcc agatgatgtc ggcgtggcaa tcatcgtatt cgtggctgtg ggcaggcaga
     1861 ccatattggt cgcgacggtc gctgtacgct acgccgataa aacctttttc gccaacccaa
     1921 gacagcccga tgctgcccgt ttgcgaatcg gcgtggctgt cgggcaggcg tttcagattg
     1981 cggtaacgcg gtacggcgta atcccccgat ttgcggtaca gcccttccgt gtgcaataca
     2041 aagtttttgc ccaaaccgat attgatgccg ccggacgtga gtttttccag attgccgctg
     2101 ctcaaacgca atccgagttc gcccgatacg ccgttttcag gcattttttc ggggattttg
     2161 ccatcggcaa catcgaccag ccccgccaca ttgcccgagc tgtacaagag cgtaaccggc
     2221 ccgcgcagga tttcgacctg ttgcgacaag gcggtatcta ccataatggc gtgatcgggc
     2281 gaaaaatccg ccatatcgcc tgtttcgccg tgatggttca acactttaat ccgcctgcct
     2341 gtttgaccgc gaatgacggg agcagacgcg ccgccgccgt attgcgaagc gtggatgccc
     2401 ggtacgccgt ctaaagcgtc gcccaagttg acggcttttt ggcgcaaggt atcgccggag
     2461 atgattttgt cggaggcggt cgaagtgtgc aacagccccg acgtggcgcg cggacggctt
     2521 ttgccgacga cgctgaccgt ttccaaatcc accgattgct cagtttcatg cgcttgggcg
     2581 aggaggggtg tgttgattaa aagaattgat aaaacaatgg gtttgagtgt agtttgtgcc
     2641 attttggctt ctcgtcgcat ttcaaaagtt tgttattata taacattaca ttttttatat
     2701 cataagattt tgagaacact cagagggcat aggcaaaagt ttttcaaatg aaacggttgc
     2761 ggcatcgggc ggtgtccatt tgtatccgcc gtccttcggg ggcgcggttg atgttgacgc
     2821 agattccgct gtgtttgccc attatgttcg gctgcgggat aaccaaaatt tatgagtgca
     2881 taaaaacggc acgttcccga acggtcgaaa aggtggcaaa atggcgtact tgtcagaacg
     2941 cgaggctctg cgccaggttc acgagggcgc atcgggcacg ctaagatata agagggtatg
     3001 gatgggggta tcggaaatgc agttaataac aaacaaatta taaatcaata ggttaatcac
     3061 aaaatgcttt tgtttatcga caattacgac agttttactt acaacatcgt ccagtatttc
     3121 actgaattgg ggcaggaagt tgccgtgcgc cgcaacgatg atattacgtt ggaggaaatc
     3181 gaggcattga atccgcaata tctcgttatc ggccccggcc cgtgttcgcc caaagaagcg
     3241 gggatttccg tggcggcgat gcgccatttc gccggccggc tgccgattat gggcgtgtgc
     3301 ctcgggcatc agacgatagg cgaggcgttc ggcggcagga tagtccgcgc caaaacgctg
     3361 atgcacggta aggtgtcgcc cgtgtcccat tcgggcaagg gtatgtttaa gggtttgccc
     3421 aatccggtta cctgtacgcg ttatcacagc ctcgttatcg atcggaatac gatgcccgaa
     3481 tgtttggaag taacggcttg gactgaggac ggcgagatta tgggtgtgcg ccataaggaa
     3541 tatgccgtcg agggcgtgca gttccacccc gaagccctct tgaccgagca cggacatgat
     3601 atgttaaaca attttttaat cgaatttcaa aacttcaaac cgcaaaaaat ctgacgtgat
     3661 gccgtctgaa gcccttcaga cggcattttc gtccgaatat tgaacggagg acaaaaaatg
     3721 attacaccgc aacaggccat cgaacgatta atcagcaata acgagttgtt ttacgatgaa
     3781 atgaccgact tgatgcgtca gattatgaga ggacaggttc tgccggagca gatagcggcc
     3841 attttgacag gattgcgtat caaggttgaa accgtttccg aaattaccgc agctgcagcc
     3901 gtcatgcgcg agtttgcgac aaaagtgccg ctggagaatg cagaggggct ggtcgatatc
     3961 gtcggtacgg gcggggatgg cgcgaaaacc ttcaatattt cgacgacttc gatgtttgtt
     4021 gccgcagcgg caggcgcgaa ggttgccaaa cacggaggcc ggtcggtctc ttcctccagc
     4081 ggtgcggctg acgtggtgga gcagatgggc gcaaacctca acctgactcc cgaacaggtt
     4141 gcccaaagta tcaggcagac cggcatcggg tttatgttcg cgcccaatca ccacagtgcc
     4201 atgcgccatg tcgcccctgt acgccgttcg ctcggtttcc gaagtatttt caacatattg
     4261 ggtccgttaa cgaatcctgc gggcgcgccg aaccagcttt tgggcgtgtt ccacaccgat
     4321 ttgtgcggca ttttgtcgcg ggtcttgcaa caacttggtt caaaacacgt tttggttgtt
     4381 tgcggggagg gcggtttgga tgaaattaca ctgacgggca aaacacgcgt tgccgagctc
     4441 aaagacggaa aaatcagcga atacgacatc cgcccagaag atttcggtat cgaaacccgc
     4501 cgcaatttgg atgaaatcaa agttgccaat actcaggaat ctttgttgaa aatgaatgag
     4561 gtgctggaag gaagagaagg ggctgcgcgc gatatcgtat tgctcaacac cgccgccgcc
     4621 ctgtatgccg gaaatgtcgc tgcttcgctt tcagacggca tatctgccgc acgggaagcc
     4681 atcgattcag gcagggccaa atcgaaaaaa gaggagtttg tcggttttca accacaacaa
     4741 agatgccatt ttcttggaaa gatggagctt gggtgatgcc gccatgatta tggaactttt
     4801 gtggcaaaac ataagcactt cacgaagaga acttaccaaa ctgtttttat ataaaaactt
     4861 ggggctgtac tagataacca gaccaaattc ccattaacta attgtcttaa aatctgaatt
     4921 tgagattcta tttaaaatgc cattggcatt tctttaaatg cagccccaaa tgctctttgg
     4981 gaatgccgtt aaacttacgt aaatggctaa attcactaat atcaagcaca tcataactac
     5041 gaaaggtatc cgtgtataca atgccatcag gcttaacttt ctttcggata attggcaaca
     5101 atgtcgctga ttgcgcatta ggaacaacga cggtataa
//
LOCUS       DAWIVB010000154         5095 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWIVB010000154 DAWIVB010000000
VERSION     DAWIVB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.154"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            186..1370
                     /gene="ubiM"
                     /locus_tag="WIX60_001722"
     CDS             186..1370
                     /gene="ubiM"
                     /locus_tag="WIX60_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ5305956.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRASNNFPPLKKLITKQL
                     TG"
     gene            complement(1559..1714)
                     /gene="rpmG"
                     /locus_tag="WIX60_001723"
     CDS             complement(1559..1714)
                     /gene="rpmG"
                     /locus_tag="WIX60_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ5305957.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1746..1979)
                     /gene="rpmB"
                     /locus_tag="WIX60_001724"
     CDS             complement(1746..1979)
                     /gene="rpmB"
                     /locus_tag="WIX60_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ5305958.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2229..3755)
                     /gene="farB"
                     /locus_tag="WIX60_001725"
     CDS             complement(2229..3755)
                     /gene="farB"
                     /locus_tag="WIX60_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ5305959.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     FVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(3779..4933)
                     /gene="farA"
                     /locus_tag="WIX60_001726"
     CDS             complement(3779..4933)
                     /gene="farA"
                     /locus_tag="WIX60_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ5305960.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         1303 a         1431 c         1215 g         1146 t
ORIGIN      
        1 accccacacc cgatttgata cacattaagc tgaaagtaaa aatccgcata caccctcccc
       61 tgcatatttc ttcaacagtg ggttttgata taatcgccta tctgttacag atagttcaaa
      121 cggcattcaa acccttacaa ataccgtctg aaatcatcgg catccgtcta tgcaaaggat
      181 attttatgag tttacacagc gacatcctcg tcgtcggcgc aggcccggcg ggtttaagtt
      241 ttgcagccga acttgccggc agcggtttga aagttaccct gatcgaacgc agcccactga
      301 ctgttctgca aaatcctcca tatgacggac gcgaaatcgc cctgacccat ttttcccgcg
      361 aaatcatgca gcgtttgggt atgtgggaca aaattcccga aaacgaaatc taccctttgc
      421 gcgatgccaa agtgctgaac ggacgttccg actaccagct ccactttccc caaccgactg
      481 aagcgcgcgg cgaacctgcc gactgtttgg gctatctgat ttccaaccac aatatccgcc
      541 gcgccgccta tgaagtcgta tcacaactcg acaatgtcag cattctgacc gatacggttg
      601 tcaaagaagt caaaacatct gacaacgaag cacaagtcat tttggaaaac ggcaaaatac
      661 tgactgcacg cctgcttttg gcggcagaca gccgcttctc gcaaacccgt cgacaactcg
      721 gtatctcctc cgatatgcac gattacagcc gaaccatgtt cgtctgccgt atgaaacata
      781 ccctttccaa ccaacatacc gcttacgaat gcttccatta cggacgcacc atcgcactgc
      841 tgccgctgga agagcacctg accaataccg tcattaccgt cgataccgat aaaatcaaca
      901 gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt aaaagaacag ctcaagggca
      961 ggctcggcga tatggaattg gtcagcagca tccaccatta cccccttgtc ggcatgattg
     1021 ccaaacgttt ctacggcaaa cgcagcgcgc tgattggcga tgccgccgtc ggtatgcacc
     1081 ccgttacagc acacggtttc aacttggggc tttccagcgc agatattttg gcaaaactga
     1141 tactcgaagc cgaacagcgc ggtcaagaca tcggtgcatc ttcattattg gaaaaataca
     1201 gcagcaaaca tatgctgcac gcccatccgc tttatcacgg aaccaatatg atgctgaaac
     1261 tgtttaccaa tgagaccgca ccggcaaaac tgttgcgcgg cttggtattg cgtgccagca
     1321 acaatttccc accgctgaag aaactgatca ccaaacaact gaccggttga aaactgccga
     1381 aatgccgtct gaaacgtttc agacggcatt tttgtgttat gaaccgtttt gcggttttca
     1441 tatccttggc agacggtttg cagcagattc gcatacggca gatgtttcaa gcggaaagga
     1501 aacacgataa aaataacaaa gcctccgagc agtcggaggc tttgctttca aactgaaatt
     1561 atttcagttt agtttcttta tacactacgt gtttgcgggc aactgggtca aattttttga
     1621 tttccaattt gccgggcata gtgcgtttgt ttttggtagt ggtgtagaag tgaccagtac
     1681 ctgcactgga ttccagtttg attttatcgc gcattgcagt aatccttaat taaatagtgt
     1741 ttaaattaag cttcgccgcg agcacgcaaa tcagccaata cgacatcaat gcctactttg
     1801 tcgatggtac gcagtgcagc gttggaaacg cgcaggcgaa cccagcggtt ttcactttct
     1861 acccaaaaac gacgtgattg caagttgggc aaaaaacggc gtttggtttt gttgttggcg
     1921 tgcgatacgt tgttgccgga catcgggcgt ttgccggtca ctttgcaaac tcgtgccata
     1981 atcagtctcc aaacttctaa aatagtaaaa cgcgatttat accacgaaaa acagtgttag
     2041 ttcaagtgtt ttaaagaaaa acggctgttt tgcgcggata atccttccga ttgtcgcatc
     2101 tgtggaacgt ttgcaggtgc gaaacagatg gggcgcgatt atagcggatt tcgggaactg
     2161 ctgcattcaa aatgccgtcc gaacatattt ccagtatttt cagacggcat ttcaagtttt
     2221 caaatccctc aatgtccacc gccgccgccg ttgtggaacg gcggttttgc cagccatatg
     2281 acgggtatca tgataatgaa taacaagctg cccgccataa agatttcgtt cgagccgata
     2341 ataaaaccct gctgggtaat ggtattgttg atgatgccta gggtttgaat gtcggaaacg
     2401 ccgtgctggg acaaatgagc ggccgtttcg tgcaatgttg cggaataggg cgtgatgtgt
     2461 tcggcaaagc gtgtgtggtg caacgcttcg cgccgttccc acagggtgct gacgacggat
     2521 acgccgacac cgcccatcag cacgcgcaag aaattcgaca ggctgcctgc ggcggcaatc
     2581 tgcccgccct tcatatgcga cagtgtgatg gtggtcagcg gcagaaaaaa catggcgaca
     2641 ccgacaccct gccaaaactg cggccagatg acgttgccaa tatccatatc ggcataaaaa
     2701 tccgtacgcc aatagaaagt aaaggcaaag gtcaggaagc tggcagttac gaacaggcgc
     2761 atatcgattt tattgccgaa cctgccgatt aacggagaca ggaaaacagg caggatgccg
     2821 acaggtgcgg cggcaagccc tgcccacgtg gaggtatagc ccaggttggt ctgcaacact
     2881 aacggcagca gggtcagcgt ccccatatac accataaaac ccaatgacgt ggcaatgacg
     2941 ccgacggtaa aattccgatc tttaaacagc gataaatcga caatcggata tttttctccc
     3001 aattcccaaa caataaaata cgacaagcac accagtgcga ctacgcccaa ggtaatgatt
     3061 tctccagagg cgaaccagtc gagttcctta cccctgtcca gcatcatctg taacgcgccg
     3121 ataccgacta ccatcaatgt aagcccgaca tagtcggtcg gcattttaac ggtttccgtt
     3181 tcccgatatt tcaaatgttt ccatgtaatc catgccgata tgataccgat agggatatta
     3241 atgaagaaaa tccaacccca atgccagttt ccggaaatcc agccgccgag tatcggcccg
     3301 agaacagggg cgacaacgac ggtcattgcc cacaatgcca gtgccagcgt ccgttttgcg
     3361 ggcggatagg atgccattaa caggctttgc gacaagggaa tcagcggccc ggcgataaag
     3421 ccctgcaaga tgcggaaaac aaccagcgac tgaaggttgg gggcaatacc gcacagccac
     3481 gatgtgatga cgaaaccgac agcggcggcg gtaaacaatt tgacctcgcc gatgcgtttt
     3541 gccaaaaagc ccgtcagcgg cacggaaacg gcgtttgcca cagaaaagga agtgatgacc
     3601 cacgtcccct gagtggttgc cgcaccgagg ttgccggcga tgacgggaac ggcgacattg
     3661 gcgatagtcg tatctaaaac ttccataaat acggcaagcc ccaaagacag cgtaacccac
     3721 gccaatgccg cacccttaag cggtggataa tccattgtgt cctccgtttc agacggcatc
     3781 agggcgcgga ttgcccgagg atttcatcga ccgtccgatc gacttccgac cagtcggtac
     3841 tttccatttc cggcaatgcc gcacccggcg tttttgaaac aggcgcgcct gcggcggaag
     3901 tatccacttt aaccgtcatc gacaaaccga tacgcaacgg atgcctgtcc acatcttcgc
     3961 ggttcagcac gatacgaacg gggacgcgct gcaccacttt aatccagttg cccgttgcgt
     4021 tttgcgccgg aatcagcgaa aacgcgctgc ccgtacctgc cgaaaaacct gccaccctgc
     4081 cgcgataaac aatttgtttg ccgtacaaat cggacaccag ctcggcaggc tgtccgattt
     4141 tcatatgccg caactgcgtc tctttaaaat tagcatccac ccacacatcc gacagcggca
     4201 ccaccgccat cagcggcgcg cctgccgcca cctgctgccc gacctgcacc gaacgcttcg
     4261 ccacctgacc gtccgccggc gcgcggattt gcgtccgctg aaggttcaac cacgcatctt
     4321 tcaacctgcc gattgccgtc tgaaccgccg gctgttcgcg caaagaaacc tgaccgccca
     4381 aagccgcacg tgccgaagat tcttccgcca aagccgcttt gaccgccgcc tgcgcctgag
     4441 acactgccgc acgggcgtgt gccagctctt cggcggacac cgcgcccgat tccgccaaag
     4501 cagaccggcg gcgcaaatca tcctgtgcgc gtgccaaatc cgcccggcgc aaggcaacct
     4561 gcgcccccgc ctgcgaagtg gcggcatttt gccggcggtt ttgccgcacc gcctgaacca
     4621 gctcgttttt tgcccgctcg taagccagca catcattatc gtcgtccaat accgccagca
     4681 cgtcgccttt tttcacggca tccgtatcgt cgtgcaaaac cttccgcacc gtaccgccct
     4741 tttgcggcgt aacctgaacc acgcgtccgg caacataagc gtcttccgtt tcctcttcgt
     4801 gctgccacca taaaaaaaac gccgacccgg cggctgcggc ggcaagcgcg aacagcagcg
     4861 tcaatgccgt caggcggcgt ttgcgtttgg cttgcgtgtt ttgaagtttt gtttcgtccg
     4921 tgtgcgtatc cataagattg ggttccgaat ttcctgattt catataaaca atgttctttc
     4981 aataggaagc agattctata tccgcgcaat aacgcaatca agtcagtatt ttagatgcaa
     5041 tattcaaaaa acaacgcttc aggcggcaaa actgaaaata tatttcgttt atata
//
LOCUS       DAWIVB010000155         4987 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWIVB010000155 DAWIVB010000000
VERSION     DAWIVB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.155"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            197..1993
                     /locus_tag="WIX60_001727"
                     /pseudo
     CDS             197..1993
                     /locus_tag="WIX60_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575393.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            2006..4945
                     /locus_tag="WIX60_001728"
     CDS             2006..4945
                     /locus_tag="WIX60_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ5305961.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFLDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFADLFTAYLHENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYCIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGR"
BASE COUNT         1509 a         1168 c         1199 g         1111 t
ORIGIN      
        1 ccctgatcta agcgacacag ccggggcgaa cactgaggca atctacactt cagacggcat
       61 taccgccaac tctacccaac tcgaacagct caaaaaactg tttcccgcct gttttgacgc
      121 agacggaaat ttccttatcg acagattaca agccgaaatc gcgccgcaga ccgacatcgg
      181 acgcgaattt tacgaaatga actggctggg caaatcatat gctcgcctgc ttcgcaacct
      241 gccgcccgaa accctgataa gtgaagacaa gacgcacaac gccaagcccg aaaatgcagg
      301 cagccaaaac ctgctgattc gtggcgacaa tctagaagtg ttgaaacact taaaaaacgc
      361 ctacacaaac agcgtgaaga tgatttacat cgacccgccc taaaacaccg gatcagacgg
      421 ctttgtctat caggacgacc gcaaattcac acccgctgaa cttgcccgcc tagccaatat
      481 tgatgaagac gaagccgcgc ggattttaga tttcaccgac aaaggctcaa actcgcacag
      541 cgcatggctc acctttatgt atccgcgcct gtatgtcgcc cgcgaactgt taaaggacga
      601 cggtgtgatt tttatctcga ttgacgataa cgaagcggcg cagttgaaat tgttgtgtga
      661 tgaagtgttt ggggaaggga attttgttgc acaattgcct tggcgaaaaa gaacagctaa
      721 atcagatgtg ccttttggta tttcgcagga ttatgaatgg atatttgtat tcgcaaaatc
      781 ttgccaattt attgcagcaa ctaaaggcaa ggaacgacgc tattatgaga ctgatgattt
      841 ccccgatcgt ccttggcgta cccacgactt aacgaaacaa acaactgcgg cggaaaggcc
      901 aaatagtttt ttcacaatgg ttgatcccaa gacaggaaaa aaatatccag caaatccaaa
      961 tgcaacttgg cgtgtaacca aagatacatt tcaagattat tataataaag gaaaaatcgt
     1021 tttccctgat gattatgatt tcttaaatat cagcaatcca gttatgcgtt actttaaaga
     1081 tgacgacatg aaaaaagctg gcgaggattt tggcaaagta gctgtaagca gtagattacc
     1141 tgaaaatgtt ggtacattag ccgatgcagt agccgaatat ttggctattt ttagtaggac
     1201 gctacctgaa aatatcggaa tgactaaaga aggcacaaaa gaaatcacag atttatttgg
     1261 tagcaaaata tttaccttcc ccaagcctag tcaattgatt aaatttttag tttcaataag
     1321 ttcaaagagt aatgacctaa tcctagactt cttcgcaggc agcggcacaa ccgcccacgc
     1381 cgtgatgcag cttaacgccg aaggacaaaa cggtaaccgc cgctatatct gtgtacagct
     1441 tcccgaaaaa accgctgaaa aatccgaagc ccgtaaagca ggctacccga ccatcttcga
     1501 catcaccaaa gcccgcatag aaaaagccgc cgccaaaatc cgcgtcgaac atcccgatta
     1561 cacgggcgat tcgggcttca aaatctttca aacggcagac aatttcaggc agcatccgga
     1621 caaggatttt tcgcccgaac aaccggattt gccgcttaac gatgaattaa gcgaagaaca
     1681 gctgcaaacg cttctgacca cctggacgct gtatgacggg gcggcactga ccacgccggt
     1741 tgagcctgtg cggttagggg cttacacggc gtatctgtgc gaaaaacggc tgtatctgat
     1801 gaatgccggt tttacttccg ccgatttgtt ggcgtttatc cgcaagctgg acgacgatgc
     1861 ggatttcaat cccaaccgcg tgattgtatt cggcagcaat atggcaagcg ccatgcagca
     1921 cgaacttgac caggcggttc gcggttatgc caataaaaaa gagattgagt tgaatgtggt
     1981 tatcagggtt tgacggaggc aattcatgag cggttttaat tacgagaaaa accagccgca
     2041 ccaaatgcgg gcggtttcgg cggttttggg cgtgtttgac ggggcaacgc ccaaatatcg
     2101 gacggcagac gaaaatcccg aacttttgtt tgctgcaaaa caatacgcaa acaatatcct
     2161 gaaagtgcaa agccaaaacg gtatagacgg ccgattcccc gaccgttcgg acgaccaaaa
     2221 tatccttgat atttccatgg aaacgggcac gggcaaaacc tatacctaca cacaaaccat
     2281 gttcgagctg caccgttggc tgggcgtgtt caaatttatc gtggtcgtgc cgactttgtc
     2341 cattaaggcg ggaacacagc agtttttgca aagcaaggct ttggcagagc attttgaaca
     2401 ggatttcggc ggcgattatg aaggcgtacg cctgaaaacc tatgtggtgg aaagcgcgaa
     2461 aaagaataag ggcaaaaagt ccaatgcgcc cataacgatt gagcaatttg tcaaagcgga
     2521 aaacaaaaag gaaattcatg tgctgctgat taacgcgggc atggttaatt cgtcgtccat
     2581 gaacgatacg ggcgacaagg cattgaagga tttgtttgac aatcccgttg atgcattggc
     2641 tgccgtgcgc ccgtttatga ttgtggacga accgcataaa ttcccgaccc gagatagcgc
     2701 gaaaacgtgg ggcaatatca aacgcttaaa accgcaatat attttgcgct acggtgcaac
     2761 atttaacgat gaatattaca acttgcttta ccgtttgacg gcagtagacg cgtttaacga
     2821 cgggctggtc aaaggcgtgc gcgtgtttca ggaagaaatg cagggcggca tggatgcggc
     2881 ggtaaaactg gtgtcgtcgg acggcaaaga agcgaaattt gaattaaacg aaaaggacaa
     2941 aaagcagacg ttcaaactgg caaaaggcga agatttggcg caaatccatc cggctatttc
     3001 ggatttgaaa atcgacaaaa tgaataaaac cgtggtggtg ttaagcaacg gcttggagtt
     3061 gaaaacgggt gccgtcatca acccttattc ctattcgcaa acggtgcagg atgcgatgat
     3121 gcagcgggcg gttgccgaac atttcaagct ggaacgcgcg cttttggcag aacgcgcgcc
     3181 acagcccaaa atcaagccgc tgacgctgtt ttttattgac gatatcgcgg gctaccgcag
     3241 cggcaacgag ctttcaggca gcctgaaaga taaatttgaa agctggattc gcgcggaagc
     3301 ggcacgccgt ctgaaaacgg aaagcgaccc gttttaccgc gattacctgc aaaagacgtt
     3361 ggacgatgta tccgcctgcc acggcggcta tttttccaaa gacaatacag acagcgacga
     3421 tagaatcgag caggaaatca atgaaatcct gcacgataag gaaaaactgc tgtctttgga
     3481 caacccgcgc cgctttattt tttccaaatg gacgctgcgc gaaggctggg acaatcccaa
     3541 cgttttccag atttgcaaac tgcgttccag cggcagcacg acttccaagc tgcaagaagt
     3601 cggacgcggc ctgcgcctgc cggtaaacga gctgatggcg cgggtgcgcg atgtaccgta
     3661 caaactgaat tattttgtcg atagcagcga aaaagacttt gtgaagcagc ttgtcggcga
     3721 aatcaacgac aattcttttc aggaagaaat ctccaaaaag tttaccgaag agctgaaaca
     3781 aaaaatattg caaaaatacc ccgatatcaa accgctggta ttggtaaacc aactgttttt
     3841 agatggcatc attgacgaca atgaaaactt tgccgaagac ggctatgaca aattaaaagc
     3901 cgcctatccc gaagccttcc ccaaaggttt ggacaaaggc aaagtcagca acgccaaaga
     3961 cgaaggcaaa gacaccatca tcatgcgcga aggcaaatat gaagaactca aagccttgtg
     4021 ggagctgatt caccataaag ccgttttgca gtataaaatc aaggatgaag ccgaatttgc
     4081 cgatttattt accgcctatt tgcatgaaaa cgctgccaaa ttcccacagg caggcatacg
     4141 cacggcggta aacgaagctt atatcaacaa cgggcttatg ctttcccgcc gcatagacag
     4201 ttttgaagat gaagatttta tccgtttcaa cacaatgact taccgagagt ttctggaaaa
     4261 actggcacaa acggcaaaaa tccgtatgca gactttgcat caggcgtttt actgcatccg
     4321 caacgaactg aacattggcg attttttgaa tatgcaaacc atcgcccaaa tcaaaaacgg
     4381 cttcaaccgg tttttgcttc atcattcctt ccataaattc gaactggatt accggcttgt
     4441 cggcagcaaa atccatccga ccaaatttac caataaagac ggcaaaccgc gcgcggtgaa
     4501 aaaagcagat ttgggcagat ttgaagatac ggagcaccgg cctgccgccg gctatctctt
     4561 cggcgagatt ttctacgatt cggatataga acatgaaaat gtcgccaaca accaaattga
     4621 aggcgtaatc gtatttacca aaataccgag aaactccatc aaaatccctg ttgccggcgg
     4681 cggcacgtat tcgcccgatt ttgcctatat cgtgaaaacc aaaagcggcg agatcctgaa
     4741 ctttgtgatt gaagccaaag ggactgacgg ggcggaagat ttgcgaaaaa gcgaagagcg
     4801 gaaaatcaaa catgccgaaa agctgtttgc cgagatttcc aaagaaatca aggtggtgtt
     4861 caaaacgcag tttgacggcg agaggatagc cgaactgatc gggcaaaata tgcccgcagg
     4921 cgggcattct gaaaacgggc gttaaagaac gaatgttcgg ggcgcgtgcc gtctgcttcg
     4981 ggatttt
//
LOCUS       DAWIVB010000156         4726 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWIVB010000156 DAWIVB010000000
VERSION     DAWIVB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.156"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            164..943
                     /gene="pgeF"
                     /locus_tag="WIX60_001729"
     CDS             164..943
                     /gene="pgeF"
                     /locus_tag="WIX60_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ5305962.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAESLGGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            995..1474
                     /locus_tag="WIX60_001730"
     CDS             995..1474
                     /locus_tag="WIX60_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305963.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1476..2474
                     /locus_tag="WIX60_001731"
     CDS             1476..2474
                     /locus_tag="WIX60_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ5305964.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2595..3014
                     /locus_tag="WIX60_001732"
     CDS             2595..3014
                     /locus_tag="WIX60_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305965.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3090..3605
                     /locus_tag="WIX60_001733"
     CDS             3090..3605
                     /locus_tag="WIX60_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ5305966.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALIAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3689..4573)
                     /locus_tag="WIX60_001734"
     CDS             complement(3689..4573)
                     /locus_tag="WIX60_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ5305967.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGSQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARKREGKKLGE
                     HLLQRLEAMEEIIDALSELFPTLLETHKEKIRARLVEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1058 a         1245 c         1327 g         1096 t
ORIGIN      
        1 aagggcatca ttcctgtacc ggcaagaatc cgaacccgaa cgtttgaaaa caatcccgaa
       61 tctccgaatt cccgcctgtg cgggaatgac gaaaaaacaa gcatccattt gccccgaagg
      121 cagttaatca accctttccg ccacacacct attccaatat ccaatgaaaa ccatcacaga
      181 aaccctaaat ctcgccccga aaggcaaaaa cttcctgacc gccgattggc ccgcgcccgc
      241 caatgtgaaa accctgatta ccacgcgcaa cggcggcgtg agccaaggtg cgtatcagag
      301 tttgaacctc ggtacgcacg tcggcgacaa tcccgaagcc gtgcgccgca accgcgaaat
      361 cgtgcaacag caggtcggat tgcccgttgc ctacctcaat caaatccaca gcaccgtcgt
      421 cgtcaacgct gcagaatcgt tgggaggcac acccgatgcg gacgcttccg tggacgacac
      481 gggtaaagcc gtctgtgctg tgatgaccgc agactgtctg cccgttctat tttgcgacag
      541 ggcaggtacg gcggttgccg ccgcacacgc gggctggcgc ggtttggcgg gcggcgtatt
      601 gcaaaacacc attgccgcga tgaaggttcc gcctgtcgaa atgatggcgt atctcggtcc
      661 cgccatcagt gcggatgcgt ttgaagtcgg acaggatgtg tttgatgcgt tctgcacgcc
      721 catgcccgaa gccgccgccg catttgaagg cataggcggc ggcaaattcc ttgccgacct
      781 ttacgcgctc gcccgcctga ttctgaagcg cgaaggcgtg ggcggcgtgt acggcggcac
      841 gcattgtacg gttttggaac gggatacttt cttttcctac cgccgcgacg gagcgacagg
      901 gcgtatggcg agcctgattt ggctggacgg caatgccgtc tgaacacgcc gctgatataa
      961 tctaccgact ttgtgctttt gagaaaggca agccatgaac aaactgtttc ttactgccgc
     1021 agtgctgatg ctgggcgcgt gcggtttcca cctgaaaggt gcagacggca tttctccgcc
     1081 gctgacctac cggagctggc acatcgaagg cggacaggca ttgcagtttc ctttggaaac
     1141 cgcgctgtat caggcttcgg gcagggtgga cgatgctgcc ggcgcgcaga tgaccctgcg
     1201 tatagacagc gtttcccaaa acaaggaaac ctacaccgtt acccgtgcgg cagtcatcaa
     1261 cgaatatctt ttgatattga cggttgaagc gcaggtattg aaacgcggcg agccggtcgg
     1321 caaaccgatg accgtgtccg tccgccgcat tttggattat gccgacaacg agatcttggg
     1381 caaacaggaa gaggaagcgg cattgtgggc ggaaatgcgg caggatgccg ccgaacagat
     1441 tgtccgccgc ctgacctttc tgaaggcgga atgacgtggc ggcacatatc ggacgcattg
     1501 atacggacgc gcctttgaaa cctctgtacg tcatccacgg cgaggaagaa ctgttgcgta
     1561 tcgaggcatt ggacgcattg agggcggcgg cgaagaaaca aggttacctt aatcgggaag
     1621 tttatacggc agacaatgcc ttcgattgga acgaactgtt gcaaaccgca ggcagtgcgg
     1681 gtctgtttgc cgatttgaag ctgttggaac tgcatatccc taacggcaag cccggcaaaa
     1741 ccggcggcga ggcgttgcag gattttgccg cccgattgcc ggaagatacg gtaacgctgg
     1801 ttttgctgcc caaactggag aaaacccagc tccagtccaa atggtttgcc gcattggcgg
     1861 caaaggggga agtgtgggaa gccaaaccgg tcggcgcggc ggctttgccc caatggatac
     1921 gcggacggct ggacaaaatc ggtttgggta tcgaggcaga cgcattggca ctgtttgctg
     1981 agcgcgtgga aggcaatctg ttggcggcgc gtcaggaaat cgacaagctc gggctgctgt
     2041 atccgaaagg gcataccgtc aatatcgatg aggcgcaaac cgccgttgcc aacgtcgccc
     2101 gcttcgacgc gttccaactg gcaggcgcgt ggatgaaggg cgatgtcctg cgcgtatgca
     2161 ggcttttgga cggattgcgg gaagagggcg aagaaccggt gctgttgctg tgggcggttg
     2221 ccgaagacgt gcggacgctg atccggcttg ctgccgccct gaagcagggg cagagcatcc
     2281 aatccgtccg caacagcctc aggctttggg gcgacaagca gacgctcgca ccgcttgcgg
     2341 tcaagcggat ttccgtcgtc cgcctgcttg acgcgctcaa aacctgcgcc caaatcgacc
     2401 gaatcatcaa aggtgcggaa gacggcgacg catggacggt attcaaacgg cttgtcgtgt
     2461 cgctggcgga ataaagcggt aatccccaaa atccgaaaat actgtaaaat accgttaatc
     2521 ctgaaaagta ttcaccaatc cgtccgaaaa catttcagac ggcacgacca cctcaataaa
     2581 ggaacattaa ccctatggac aataagacca aactgcgctt gggcggcctg attttactga
     2641 ccaccgccgt tttaagcctc attatcgtat tgattgtcga ttcctggccg cttgccatcc
     2701 tgcttgcagc cgtcattgtc gctgccgctg cgggcggttt tgtttggaca tcccgccgac
     2761 agcaacgcca gtttatcgaa cgcctgaaaa aattcgacat cgatcccgaa aaaggcagaa
     2821 tcaacgaggc aaacctgcgc cgtatgtacc acagcggcgg acaacaccag aaagatgcga
     2881 ttaccctgat ctgcctgtcg caaaaatgtt cggtggacga ggcgcacgct atgttcaaaa
     2941 aacgcccgac acgtcaggaa atcaatcaaa tggcggcaaa acagtcgcgc ggtcagaaac
     3001 gtccgcaccg ttaaccgccg caaggcatct ttgcataaat gccgtctgaa gcctgttggc
     3061 gtttcagacg gcatattctg attgaaaaga tgatgacact gaaaaccgcc ccgctcaaac
     3121 gccgctttgc cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag
     3181 cattgattgc cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat
     3241 tggtaacaag tatcctgata atgggagcat ggtggcttta tttccgcgcc aactggcatg
     3301 gtcaggggca gaccttggcg atgaggacat ggaaaatcgg cttgtgcgac cttaacggca
     3361 tacagccgtc tttgcacctg ctgcgcctgc gctttatttg ggcgtgcata tttatcgtat
     3421 ttatccctat gttagcctat gccggattac gccacttcct cggcattccg cccaagggcg
     3481 cggccggcgc ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg
     3541 atcggcagtt tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc
     3601 cttaagcctt tataccgcaa aggtttcaac ctgaaaaaat gccgtctgaa agggctttca
     3661 gacggaattt gcttatcggg gaaaccgatt attcgatatt ctgcacttgt tcccgcatct
     3721 gctcgattaa gactttcagt tcgaccgagg cttgggtgca ttcggcggca atggatttgc
     3781 tgcccaaagt gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgtttgccgc
     3841 tgctgccttt gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgtaggcggc
     3901 tgaactcttc gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt
     3961 cgttgtcgat gctgccgacc gcttcgacga ggcgggcgcg gattttttct ttatgtgttt
     4021 ccaacagggt aggaaagagt tcgcttaatg catctatgat ttcttccata gcctcaaggc
     4081 gttgcagcag gtgctcgcct aatttcttac cttcccgctt gcgtgcggca gtaaagtctt
     4141 ttaacgcttt ttcggtcagt tcggtaatgc tttttgccaa ttcttccgta ttttcccttt
     4201 ggcttgccaa tacgccgggg aaacgcagga tgtcggcaac gcccagtttt gccaaatcgt
     4261 gatgcttgcg gaggtctttg ttgatttcgg caagctgtcc gaccaagtcg cgattcagtt
     4321 ccaaggactg actgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc
     4381 gtgcgatatg ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcgtcgg
     4441 gcatcctgat ttgaatatct agaaaacggt ggttgaccgc acgcaggtcg agattgatgc
     4501 gtttgctgcc gcactctgcc gccgcgttgg caaatccggt catgctgtgg atgtggatat
     4561 ttccgctgct catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt
     4621 atggcggcaa gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc
     4681 acgcgctttt gttgctgcaa ggaagggaaa tgtatagtgg attaac
//
LOCUS       DAWIVB010000157         4654 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWIVB010000157 DAWIVB010000000
VERSION     DAWIVB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.157"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            116..1507
                     /locus_tag="WIX60_001735"
     CDS             116..1507
                     /locus_tag="WIX60_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ5305968.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(1569..2321)
                     /gene="rlmB"
                     /locus_tag="WIX60_001736"
     CDS             complement(1569..2321)
                     /gene="rlmB"
                     /locus_tag="WIX60_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ5305969.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(2388..2876)
                     /locus_tag="WIX60_001737"
     CDS             complement(2388..2876)
                     /locus_tag="WIX60_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ5305970.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(2881..3600)
                     /locus_tag="WIX60_001738"
     CDS             complement(2881..3600)
                     /locus_tag="WIX60_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ5305971.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMSQAHKARLLAELKNHPPVAAFPPQEEMEHFMCEALRQ
                     AEQSSADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGREIQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(3604..4545)
                     /gene="miaA"
                     /locus_tag="WIX60_001739"
     CDS             complement(3604..4545)
                     /gene="miaA"
                     /locus_tag="WIX60_001739"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ5305972.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSLTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTAYSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDCTSCTRLIEAAKRFFGA"
BASE COUNT          865 a         1115 c         1506 g         1168 t
ORIGIN      
        1 aatgtggtaa acgtgtggac cagacggatg ccgtctgaaa tgcaaattga agccgtgcgg
       61 cagattcgct acaatccgcg cttggatttt tcaaccttta aaataaggaa atacaatgag
      121 cggtcagttg ggcaaaggtg cggatgcgcc tgatttggtg tacggtttgg aagacaggcc
      181 gccgttcggt aatgcgctct tgagcgcggt tacccatctt ttggcgattt ttgtgccgat
      241 gattacgccc gcgctgattg tgggcggcgc gctggaattg ccggtggaga tgacggcgta
      301 tctcgtgtcg atggcgatgg ttgcgtcggg tgtcggcact tatttgcagg tcaaccgctt
      361 cgggccggtc ggttcgggga tgctgtccat ccagtcggtg aatttttcgt tcgttaccgt
      421 gatgattgcg ctgggcgcgg ggatgaaaga gggcggtttg actaaggatg cgatgatttc
      481 gacgctcttg ggcgtatcgt ttgtcggcgc gtttttggtg tgtttctcgg cgtggcttct
      541 gccgtatttg aaaaaagtga ttacgccgac ggtcagcggc gtggtcgtga tgctcattgg
      601 tttgagtttg gtacacgtcg gcattaccga tttcggcggc ggcttcggcg cgaaggcgga
      661 cggcacgttc ggctcgatgg aaaacttggg gctggcatcg ctggtgttgc tgattgtgtt
      721 ggtgttcaac tgcatgaaaa acccgctgtt gcgcatgagc ggcattgcgg tcgggctgat
      781 tgccggctat atcgtcgcgc tgtttttggg caaggtggat ttttccgcgc tgcaaaacct
      841 gccgctggtt acgctgcccg taccgtttaa atacggtttt gctttcgact ggcacgcgtt
      901 tattgtggcg ggcgcgattt tcttgttgag cgtgtttgag gcggtcggcg atttaaccgc
      961 gacggcaatg gtgtccgacc agccgattga aggcgaggaa tacaccaaac gcctgcgcgg
     1021 cggcgtgttg gctgacggct tggtgtcggt gattgcgacg gctttgggtt cgctgccgct
     1081 gacgacgttt gcgcaaaaca acggcgtgat tcagatgacc ggcgtggctt cgcgccatgt
     1141 gggcaaatat attgccgtga ttttggtgct gttgggtctg ttccccgttg tcggtcgcgc
     1201 gtttacgacg attccgagtc cggtgttggg cggcgcgatg gttttgatgt tcggcttaat
     1261 tgcgattgcg ggcgtgcgga ttttggtcag tcacggcatc cgcaggcgcg aagcggtgat
     1321 tgcggcaacg tcggtcggtt tgggcttggg tgtcgcgttt gagccggaag tgtttaaaaa
     1381 cctgcccgtc ttgttccaaa actctatttc cgccggcggc attacggcag tcttgctgaa
     1441 tttggtcttg cccgaagata aaaccgaggc ggcggtcaag tttgataccg accacttgga
     1501 acactgattt tgaaaatgaa tgccgtctga aacagaatcc ctgtttcaga cggcattgtt
     1561 tttgaggctt atactttttc gttttttaat acgcgttgtc ggcgtgtttc acttaatacc
     1621 attccggcag acacggagac gttcatgctt tcgactgtgc cgaacatggg tatagacacc
     1681 agcatgtcgc aatgttcgcg cgtgaggcgg cgcataccgt cgccttcgtt gcccattacc
     1741 cacgccgcgc tgtcgggcag attgcaatgg taaaggtcgg actcgccgct catatcggtg
     1801 ccgataatcc aaatgccgta ttctttcaat tcgcgcaggg tgcgggcgag gttggttacg
     1861 gtgatatagg ggacggtttc cgccgcaccg caggcgactt tgctgacggt ggcgttcagc
     1921 cccgcgcttt tgtctttcgg tgcgatgacg gcgtgtacgc ccattgcgtc ggcggtacgc
     1981 aggcacgcgc cgaggttgtg cggatcggtg atgccgtcga gtatcagcag cagcggcggt
     2041 tcgctgaggt tttccaatac gtcttcgagg tggacgtggt ttttggaggc atcgataaat
     2101 ccgaccacgc cctgatggcg cgcgcctttg ctgatggcgt tgaggcggtc ggcatcggca
     2161 aaatatacgc ggatgttttc gtttgccgcc ttttccaaca cttcgcgcgt gcgtgcgtct
     2221 gatttgcctt cttggatgta gagttcgacg atggatttgg ggttttgcca caatcgggcg
     2281 ttgacggcgt ggaagccgta gatgggtctt tggtttgcca tgatggtgct ttgtaaaaag
     2341 ggttcagaca gcattatagc aatttgccgg tatgccgtct gaaagggtta aaacaggtag
     2401 gcgatgtatt tcaccaacag gataaacaag atggatacgg cgcagccgat tttgaacgcc
     2461 gtgccgacga caagccccaa cagcgtaccc aagcccgctt tacctgcctg aagcatattg
     2521 cgccgttcga tcagttcgcc tgccgccgcg ccgataaagg gaccgagtat tagtccggga
     2581 agggagaaaa atatgccgat gatgctgccg gccaatgcgc cgcgaacggc gagcttgccc
     2641 gctccggtat attttgtccc ccatatgcct gccacatagt ccgccagtat gccggcaagg
     2701 ctgatgagtc cgaccgtcca caaaacgccc gcgccgtaga tttggtagcc gccggcatag
     2761 gcaagcagcc atgttccggc aaacatcaat gccaatccgg gcagggcggg gtaaacgatg
     2821 cccgccgtgc cgacggctat cagggcgagg gcgaggatga cggtcagtac ggtcataggt
     2881 tcaacctttt cttttgtttt gaaaaaaacg gcttaacacg gcgcggcatt cttcttgcag
     2941 gattccgccc cgtatggcgg tgtgcgtatt gaggcgtttg tcggcaaaca ggttgacgat
     3001 gctgcctgcc gcgccggttt tgggttctgc cgccccgtag atcacacgcc tgattcgtgc
     3061 ctgtatcagt gcggacgcgc acatggcgca gggttcgagg gtgatatata tgtcgcatcc
     3121 gtcaaggcgg tagttttgta tttctctgcc tgcctgtgcc aaggcgttga tttcggcgtg
     3181 tcggctgaca ttgcagtcgg caatgcaggt gttgtgtgcc gatgcgatga ttttgccgtc
     3241 tgaaacgatg actgccccga cgggtatttc gccgtcggcg gaggattgtt ctgcttggcg
     3301 cagtgcttcg cacatgaagt gttccatttc ttcctgcggc ggaaaggcgg cgacgggcgg
     3361 atggtttttt aactcggcaa gcaggcgggc tttatgcgct tgggacattt cttgcggcgg
     3421 cgtgccgtcc agcagcgact cgagttgcca cagtgtgctt ttcgtgaggg tcaaacccga
     3481 tgctttgagc agcagaaagg ctttgaccga accgttttgc cgcagttctt cgagtgtacg
     3541 gataccgagc ctgtgcaggg cggcgacggt tttgggggcg agcggcggtg tggtcagcat
     3601 ggtttatgcg ccgaaaaacc gttttgccgc ctcaatcagg cgtgtgcatg aagtgcagtc
     3661 tgaaaacggg tcggcaacgc agtctaaagg tgttttgcgc aaccaagtca gttggcgttt
     3721 ggcaagttgg cgggtggcgg caatgccttt ctcgacaaat gccgggaaat cggtttttcc
     3781 atccagatat ttccatgcct gacggtagcc gacgcagcgg atggcggggg agtaggcggt
     3841 caggccggga tagcggcggc gcaggttttc tacttcgccg ataaagccct gttcaagcat
     3901 caggtggaaa cgcagggcga tgttttcatg caggcgggca cggttttcgg gaatcagggc
     3961 ggcggtatgc aaatcaaaag ggagcgtatg ggaggtcagg ctgccgagat gtgtgctcat
     4021 cggtttgccg gttaaataat aaacttccaa agcgcgtccg atacgctggc tgtcgttcgg
     4081 tttcagacgg catgcggttt cagggtcgac tttttgcagg gtgcggtaga ggaaatccaa
     4141 gccgtacatc tgtttttgtt cgtccaagtc ggcacgcagg caggcgtcgg cttcgggcaa
     4201 atcgttcaaa ccttgggtca gggcgcggaa atacatcatc gtgccgccga caataagggc
     4261 aaacctgccg cgtgaggaaa tttccccgac caagcgcgtg cagtcttcga caaagcgggc
     4321 ggcgctgtat gattcggtag gcgggatgat gtcgataagg tggtgcggga caaaggcgcg
     4381 ttcggaggcg gacggtttcg ccgtgccgat gtccatatcg cggtaaacca gcgcggaatc
     4441 gaggctgatg atttcgacag gcagggtttc ggcaattttg agggcgagcg cggttttgcc
     4501 tccggcggtc ggcccgagca gggcaaaggc tttcggggtc ggcataacgt ttcaggtttg
     4561 gaaaaatacg gattatagcg gaaagcgtgc cgacgttata ttttggtttg cggaagcacg
     4621 ccgacggcaa gggggcgtgt ttaccgtatg cctt
//
LOCUS       DAWIVB010000158         4611 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWIVB010000158 DAWIVB010000000
VERSION     DAWIVB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.158"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(67..291)
                     /locus_tag="WIX60_001740"
     CDS             complement(67..291)
                     /locus_tag="WIX60_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ5305973.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(292..1680)
                     /locus_tag="WIX60_001741"
     CDS             complement(292..1680)
                     /locus_tag="WIX60_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ5305974.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAVAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WIX60_001742"
     CDS             complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WIX60_001742"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ5305975.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2594..3037)
                     /locus_tag="WIX60_001743"
                     /pseudo
     CDS             complement(2594..3037)
                     /locus_tag="WIX60_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
     gene            complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="WIX60_001744"
     CDS             complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="WIX60_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ5305976.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT         1045 a         1403 c         1223 g          940 t
ORIGIN      
        1 ttaggtttgc atcggcggaa tattcaaaca cagccttttt taaggaaatc cggatacggc
       61 ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa tacggctctt gcgcgtcggg
      121 attcctgtcc tgcatttttt gatacagcag cctcaactga gcctgctgca aatccagcgt
      181 ctgccgcaat tccgccacca tcgcgttcag gccggcgatt acgtcctcct gaagcgcgga
      241 ttggatttcc agttcgacaa tacggcgttc caactcttga accgcgtcca tttacagcac
      301 catcgcggca atccagccgg caatcagcag cgggatgttg tagtggatga aggtcgggat
      361 aacggaatcg cggatgtggt cgtgctgccc gtcggcgttc agccccatcg tcgggcccag
      421 cgtggaatcg gacgcaggcg aaccggcatc gcccaacgcc cccgccgtgc cgacaatggc
      481 gacggtggca agcggcgaaa aacccaaacc gacacacaaa ggcacataaa tcgcggcaat
      541 aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt accaaaagcc ccaccaccag
      601 catcgccaat gccgccatac ctttgctgtt gccgaatatc gccatactgc tttccaccag
      661 cggctgaata tgcccggtcg cattcatcac ggcggcaaaa ccctgcgcgg caatcataat
      721 gaagccgacc atcgccatca tcttgatacc ttcgccgaat acgtcgtttg ccttgtcgcg
      781 gttaatgacc cccaacatca taaatacggc gaaaccgagc atcgcgccca acaccagcga
      841 gtcttcatac atcaactgga tggcaaagca tacggcaatg gcgacggcgg cggccaggct
      901 gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg ttgcccgccg tatcggcatt
      961 gttgctttgg tacaggcgcg gtttgcggta atggacaaac gccagcagga gtccggccag
     1021 cattcccaac gcgggaatcg ccattgccgc catcacgtta atgtttttca catcaagctg
     1081 cggcgcggcg gaatggatgt tgcccaacag gatttcgttc aaaaaaatcg cgccgaagcc
     1141 gtaaggcagg aacatataag tcgtaaccag cccgaaagtg atgacgcacg caatcaggcg
     1201 gcggtcgatt ttcaggcggt tgaacaccaa aagcagcggc ggaacaatca tcgggataaa
     1261 ggcaatgtgg atggggatga tgttctgact catcatgccc atcacaagga tgatggaaag
     1321 cagcagccat ttgaccgcgc cctcgcccga acgcacgctg tcgggcatac cgccccggtt
     1381 cagcttgcgg acgaccgcgc cggcaagctg ctgcggcagg ccggaatggg taatcgccat
     1441 tgcaaacgcg ccgagcatcg cataagaaag cgcaatcttc gcaccgcctt ccaaaccttt
     1501 gttgaacacg gggataatcc ccgcctgact gacctgtccc gccgcatcgg caatgttttg
     1561 cagcggcata cccgccaccg cgccgccgac aaacgcgccg accgtcaggc tcaataccac
     1621 gtgcacgcgc gacagcgaca gcaccagcat aacgattacg gcaactacga ctgcattcat
     1681 tgtttatcgc tccaaaccta taaatgttta catatcgaaa cacatcataa cccaataacg
     1741 ggaaacccgc caattttgca aacaattatt tcaaatgctt catatacttc cccagcgtaa
     1801 ccctgtccaa acccgccaaa tccggcaggg tttccactcc tgaaaaacca ttctccgcca
     1861 acacgccgcg caccgccgcg ccctgatcga aaccgtgttc cagcaataaa aaaccgcctt
     1921 ccgccaaacg gtcgggcgcg ccttgcgcca aggtgcggat gcagcttagg ccgtctgaaa
     1981 agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc ttgcaacaaa tgtttatcgc
     2041 cgttttcgat atagggcggg ttggacacga tgatgtccca tttcccttca gacggcatat
     2101 cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatcc gccgcatttt
     2161 tccgcgccgt ttcaagggcg ggcgggctga tgtcggatgc gcgcacaaac gcatcggggc
     2221 gttcgagcgc gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc
     2281 cgttttcggg caggcgcgcc aatacggctt cgaccaaatg ttcggtttcg gggcgcggaa
     2341 tcagcacgct cggattgact gtaaagcgtc tgccataaaa ttcgcgcaca cctaaaatat
     2401 aggcaaccgg ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt
     2461 cgtccggcat ttcttccccg ccccgcgtca acaactgcac gcgcgtatat tccgaaacat
     2521 attgcagcag cattcttgct tcatttttag gcagttttga caagcccaac catttatcaa
     2581 acgtcatttt tatcccgtct gccgctgatg cggcttttct ttccttattc tttccggcaa
     2641 acgtaccgat ggtggcaacc gcaaatgcgg cataccacaa ataaaatcct gcaccgtagc
     2701 gcacaatatc cgatgtattc cctgcttcat cgacgtatac ggctttcaca ctgaaagcca
     2761 ccaacgccaa gccccaaagt gccgcatgga caggcacgac cttcttccgc aacgccagca
     2821 aaacaatggc cgccaaccaa acataattcg catagaccgc acaatacctg atatccaaag
     2881 aagcaaatat cgaccccaaa atcaaaacgg tcaaaccctc catgcttcca tgattgccca
     2941 aataaaatgc aacattggat aaagacgcta tccacagggc aaccgacacc agcaacatca
     3001 ctatgggaaa acttggtttc cgattctgtt cctgcatggt tttatcctaa tgtaaaaggc
     3061 cgcctgaaaa cctttcagac ggcatcgtgc cggattccgc gtcagattgc gctgccgccg
     3121 acggtcagtc cggcatcaat ccgcaaagtc ggttgcccca cgccgacggg gacgctctgc
     3181 ccttctttgc cgcacacgcc gacaccgctg tcgagcgcag tatcgttgcc tatcatggaa
     3241 acgtgtttca gcacttcggg gccgttgccg atgatggtcg cgcctttgac gggatattgc
     3301 agcctgccgc cttccaccca ccacgcttcg gacgcactga acacgaactt gccgctggta
     3361 atgtccactt gtccgccgcc aaagttgacg gcgtaaatgc ccttgtcgat ggacgcgatg
     3421 atttcttccg gctcatagct gccgttttcc ataaaggtat tggtcatgcg cggcataggg
     3481 gcggaagcgt aactttcgcg gcggccgttg ccggtggact gcgtacccgt caggcgggca
     3541 ttggtttcgt cctgcatata gccgactaaa atgccgtctt caatcaatac ggtgcggcgg
     3601 gtttcgttgc cttcgtcgtc gatgttgagc gaaccgcgcc ggccggcaat atcaccctga
     3661 tcgacgacgg taacgccttt ggcggcgacg cgctcgccta ttctgccgga aaagacgctg
     3721 gttcccttgc ggttgaaatc gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg
     3781 cccggccagc cgttgcccaa aacgacggtc atttcgccgg cgggcgcggg gcgggattcg
     3841 aggttggtga gtgcctgttt gacggcggca tcgacaaacc gatgaaccaa gttttcatcg
     3901 aaataagcca agtcgtagcg tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc
     3961 tgtttggcga taacggtaac gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg
     4021 ccgtccagac gggcgaggta aaccatatcg tattcgcagg tcaaaccggc catcacttgc
     4081 acgatgcgcg gatcggcggc tttggcgatt gcttccactt tgttcaacag cgcgactttg
     4141 gcggcggaat cgaggccggc aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt
     4201 tcagacggca ttttggcgga caccttgccg cctgccgccc caatcgcgcg gacggcgcgg
     4261 gcggaacggt ttatcgaatc gatgcacagg ctgtcggcgt aggcaaaggc ggttttgtcg
     4321 cccgaaacgg cgcgcacgcc cacgccctga ttgatttgga agctgcccga tttgacgatg
     4381 ccctcttcca aatgccagct ttcataagcg gtgcgctggc agtagatgtc ggcgtaatcg
     4441 acgtggtgcg cgccgatgat gcacaggctt ttggcgagca gttcggggga aaggcggttg
     4501 gcttcgagca gccgcgcctg tacggcggaa taggtcggat gcatagtgtc ggcgcataaa
     4561 aaatcagggg cttgattata cggcatttgt tatatagtgg attaacaaaa a
//
LOCUS       DAWIVB010000159         4577 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWIVB010000159 DAWIVB010000000
VERSION     DAWIVB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.159"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(279..386)
                     /locus_tag="WIX60_001745"
                     /pseudo
     CDS             complement(279..386)
                     /locus_tag="WIX60_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(432..1160)
                     /gene="pdxJ"
                     /locus_tag="WIX60_001746"
     CDS             complement(432..1160)
                     /gene="pdxJ"
                     /locus_tag="WIX60_001746"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ5305977.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQIRQFERIQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1184..1975)
                     /gene="recO"
                     /locus_tag="WIX60_001747"
     CDS             complement(1184..1975)
                     /gene="recO"
                     /locus_tag="WIX60_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ5305978.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLWVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSVSWYGSQELKTLHRAEWVGGWRQPQGRALFGGLYVNELVLK
                     LTAREDPVPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTVESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(2012..3139)
                     /gene="pheA"
                     /locus_tag="WIX60_001748"
     CDS             complement(2012..3139)
                     /gene="pheA"
                     /locus_tag="WIX60_001748"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ5305979.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEIVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3178..4410)
                     /locus_tag="WIX60_001749"
     CDS             complement(3178..4410)
                     /locus_tag="WIX60_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ5305980.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFVSSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         1023 a         1313 c         1256 g          985 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat tacataatca tcgcctcaat
       61 atttaatatc ccaaaatata gatttaaaac catcgccttt gtggtttgtc agcattccta
      121 attgagatag aaagtcaaat aatttcttgt ttctccggct gccatcaaga tggtttagat
      181 taagacctcg aacaatacaa tacccaatac tctgtactgt attgtgaatt tcttctatca
      241 aactattatt gtttgcagat aaaatatact tttgattcat atagtgaatt aaatttaaac
      301 cagtacggcg ttggcttgcc ttgccgtact atttgtactg tctgcggctt cgccgccttg
      361 tcctgattta aatttaatcc actataacat atccctgtcg tgaaatgctg tctgaaaggg
      421 tttgcctgcc cttacggcag cagccttgcc ctgaacatcg cctccttcat ttggcgcacg
      481 gcttcgggca gtccgaggaa gagggcttgg gcaatcagcg aatgcccgat gttcagttcg
      541 cggatggcga ggatttgggc gatgggggta acgttgtgta tggtcagtcc gtgtccggcg
      601 ttgacgacca agcccaaatc gccggcgaaa tgcgcgccgt tttggatgcg ctcgaactgc
      661 ctgatttgtt cggcgtggct gcgcgcgtcg gcatacgcgc ctgtgtgcag ctcgacaacg
      721 ggcgcgccga catcacgggc ggcttggatt tgcctgtcgt cggcatcgat aaacaaagac
      781 acgcgtatgc ctgcgtcggt caggattttg gtgaacccgg cgattttttc ctgttgcgcc
      841 aatacgtcca aaccgccttc ggtcgtgatt tcctgacgtt tttcaggcac gatgcacacg
      901 tcttccggca tcactttcaa agcgttttcc aacatttctt ccgtcaacgc catttcaagg
      961 ttcaggcgcg tgcggatggc gtttttgacg gcaaacacgt ccgcgtcttt gatgtggcgg
     1021 cggtcttcgc gcaggtgcat ggtaatcaaa tccgcaccgt gcgtttcggc aaccagtgcc
     1081 gcctccacgg ggctgggata agtcgtaccg cgcgcattgc ggacggtggc gatgtggtcg
     1141 atgttgacac ctaaaagcat aatctttcct tttatttctg ccttcagacg gcatttgaag
     1201 ccgtgccgtc cgaagtcggg acggtttccc gggcggtttc tttgcggtca aactgccgta
     1261 tctgttccaa cacctgccgc gatttcagcc cctcgggcaa caggtggcgg ataaaaagcc
     1321 gtgtgatttt caatgcctgt tgcaggcttt cgacagtgcg gaaactgcct tcgcgcaaat
     1381 cgatcaggct ctgcccgggg gcgacaacgc cggcggcgtg cggcggtacg gcaaatcctt
     1441 ttccgacggg gaagacggct gtttccgggc ggacaaggta tgtgccgcct gccgcaatcg
     1501 tcccgccgtc cccgtcgcgg ttcaaatcgg gggcaacgcc caacaggttc agcagccgcc
     1561 actcgaaacg gcgcaagtcg tcgatataag cggctttgca gcacaccgcc tccatcactt
     1621 ccgccaacgc gtcgtataac tcgggcaccg ggtcttcgcg ggcggtcagt ttcaacacca
     1681 actcgttcac atacaatccg ccgaacaacg ccctgccctg aggctgccgc caaccgccga
     1741 cccattcggc gcggtgtagg gttttgagtt cctgactgcc gtaccacgac acgctgacgg
     1801 gcacgaacgg caccaatacg ccgcgcagct cgctctgcct tttgcgcgcg ctgcgcgcca
     1861 gcaaagccac acgcccgtaa cggcggctga atgcttcaac ccacaggctg ctttcgcgcc
     1921 agggcgaaga tgccagcata aaaacgggtt catggttgac tcggtattcg gacataatcg
     1981 ttggggaaat gccgtctgaa cgctgccgcc gctacaaaac ggcggtcggg tacgaaccga
     2041 tgactttgac gaacgaagcg cgttcgccca agcgttccaa tgccgtctga atctgcgcgt
     2101 cccggcggtg tccttcgatg tcgatgaaga acaggtattc ccacaaaacg gatttgctcg
     2161 gacggctctc aaacttggtc atggaaatac ccgattcggt cagcggttgc agcagcgagg
     2221 caaccgcgcc tgcccggttg ggcgcggaaa cggccagcga agtcttgtcg ctgccgcttg
     2281 caccggtttc gtgatgtccc atcaccaaga agcgcgtggt gttgttcggt tcgtcttcga
     2341 tgcactcggc aaccatatcg agtccgtaga tttccgccgc cgtgcgtccg gcgatggcgg
     2401 caaccgtacc gtcgtccgat tcggcaacca gccttgcggc ttcggcattg ctggacacgg
     2461 caatccgttc ggcgttgggc aggtgtctgc ccaaccagtc gttgcactgc gccaacgcct
     2521 gcgcgtggga aaagactttg gcaatgcctt cggtgctgcc gttgttttta cgcaaaaggt
     2581 tgtggtggat gcgcaaaacg atttcgccgc acgcctgcaa cgcggtaacg gcaagcaggt
     2641 ctaacgtgcg accgaccgag ccttcggtcg aattttccac gggggcgacc agataatccg
     2701 cctgacgcgt ttcaacctgc ttgaagcagt cgtctatggt cggacacgcc atggtgtgcg
     2761 cggcgtgtcc gaaatgtttg attgccgcct gctgggtaaa cgtgccctgc ggccccagat
     2821 aggcgatggt cagcgggcgt tcgacggcga ggcactcgct catcacttcc cgaaacaggc
     2881 gtgctaccga ttcgtcgggc agcgggcctt tgttcaaatc ctgaatgcgg cgcaacacgg
     2941 cgacttcgcg ttcggggcgg tacactgcgc ccgtgccttt cagctcgccg atggcgtggg
     3001 cgtgttgcgc acgttcgttg agcaggcgca ggatttcggc atcgatggtg tcgatggcgt
     3061 tgcggtgggg aaggaggagt tcgtcgatag tttgggacat aatttcgcct gaacagtaat
     3121 ttatcgtgca ttctaacatc aaatgccgtc tgaaacggtt tcagacggca ttttccgtta
     3181 aaggtattcg ctttgcccgt tttctttcca cgcacgatgc gagcagagcc acagaagcgc
     3241 aatgccgcaa gaggtggacg cggtcatcgt tgccgccatc acggttgccg aaccgtcgtg
     3301 caagaaggtc gccgccatac ccacccccgc gccgattaaa gattggaata cacccaatac
     3361 ggcgtttgcg ctgccgccct cttctttgaa ataggacata aaacacgcct gcgtgtttgc
     3421 accgaccaag ccctgcgtac cgacggaaaa catcacgcac gcgaccagca gccaaaacgg
     3481 gggcaacccg aaaaacagca cggcggcgag ttgggacagg ttggcggcaa actggacgac
     3541 aatcccccac agcaggatgc tttgcggatg cacgccggtt ttgagccgcc acgcggtaac
     3601 gcggttgaaa aacatcatcg tgatgatgtt gagtgcaaac gcccaagcgt attgatgagg
     3661 cgtaacacgg tagagctgct ggtacacgaa ggaagattcg gtcagaaagg cgaacatcga
     3721 accgaagctg aatgcctgaa aaaacagata acccatcgca gcacgggttt tcaatacgcg
     3781 cttgaaccgc cccgccacca gcccgaacac gtcccgtccg attttgccgc cgacggcggg
     3841 cttgggcagg aaatactgta ccaaaccgag cagcaccagc gaatacgccg ccagaaaaac
     3901 aaaaatcgcc tgccagccac ccaagccctg caacaatgcg ccgaccatgg gtgcgaccag
     3961 cggcacaacc atcaaaatga tgccgataag ggcaaacatc tgggcggctt tgcgtccgga
     4021 ataataatcg cgcaccattg cgccgacgat gaccacagtc atgcccgcac cgaatgcctg
     4081 cacgacgcgc aggttgagga gctgttcggc actcgaaaca aatacgatgg cggcaacggc
     4141 aaggcaatat acaatcaaac cggtcagggc gacgggtttg cgccctttga tgtcggacac
     4201 cgaaccgccg accacctgtc cgaacgccgt gccgaacata aacaaactca aactctgttc
     4261 gatgcggtga acatccgcgt tcagcgattg cgccatttcg ggaatcgcgg gcaggtaggc
     4321 atcgatggaa aacggcatca gcgtaaccag catcgccatc aaaaccgcca tcagtttttc
     4381 gctcatttca ggataatggg cagaaggcat aaattctcgt ccgattcgca aacaaaccgc
     4441 ccggcggcgg aaaacacaat acgcggaaac ggaaaatttt aacacaccgg cacaaacagg
     4501 cggcaacaaa agccccggca aaaccggacg aaccggaacg atatagtgga ttaacaaaaa
     4561 ccagtacagc gttgcct
//
LOCUS       DAWIVB010000160         4505 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWIVB010000160 DAWIVB010000000
VERSION     DAWIVB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.160"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(103..1041)
                     /locus_tag="WIX60_001750"
     CDS             complement(103..1041)
                     /locus_tag="WIX60_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ5305981.1"
                     /translation="MLELNGLCKRFGNKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NIIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHKGRILQYGTPET
                     LVKTPSCVQVARLMGLPNTDDNRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1050..1925)
                     /locus_tag="WIX60_001751"
     CDS             complement(1050..1925)
                     /locus_tag="WIX60_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ5305982.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWVERVKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLALGWHCAGLLDGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPNK
                     LDNTAAGGVSGGEMPSEAVCRESSEEAGLDKTLLPLIRPVSQLHSLRSVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2121..2197)
                     /locus_tag="WIX60_001752"
     tRNA            complement(2121..2197)
                     /locus_tag="WIX60_001752"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2161..2163),aa:Met,seq:cat)
     regulatory      2347..2446
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2539..4440
                     /gene="thiC"
                     /locus_tag="WIX60_001753"
     CDS             2539..4440
                     /gene="thiC"
                     /locus_tag="WIX60_001753"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ5305983.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGICFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLEQHAGQSFGANIPTHPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1090 a         1296 c         1186 g          933 t
ORIGIN      
        1 gtttcggata aaattttgca gccctgataa aaaaatatgg ctgctttggt aaaaaaatgc
       61 cgtctgaaag gttttcagac ggcattttgt ttttaagaag catcagcgga agcggacgat
      121 ttcccgttct tcgatatgga tgcgtaccgt atccttgccc gataccgccc cggcgtgccg
      181 catatcgagg ttcagccaca ggatgccgtg ttccggatgg aggacggaca ggctgaacga
      241 ttcgggcaaa caggtacggg ataatacgcg gcactccatg ccgtcttggt cgaaacgcac
      301 cgcatgttgc ggaatatggc ggttatcgtc ggtattgggc aaacccatca gtcgggcgac
      361 ctgcacgcag gatggtgttt tgaccaatgt ttcgggcgta ccgtattgta gaatcctccc
      421 tttatgcatc acggcgattt cgtctgccgt cgtacaggct tcttcgggcg aatgcgttac
      481 caaaacggca gggatgccgc cgtttcggat acgttcggca gtcatacggc gcagcgtgcc
      541 gcgcaaatgc gtgtccaaac tggaaaacga ttcgtccaac agcagcaggg aagggcggac
      601 aaccaaagcg cgcgccaacg ccagccgttg cttctcgcct ccggaaagtt tttcaggctt
      661 gcggtgcgcc tcgttttcca gtccgacttc ggcaagtgcc gccatggcga ggcgttcggc
      721 ttcggctttc ggcatttttt gcattttcaa accgaatgcc gcattttcca gcgcactcat
      781 atggggaaac agcgcgtaat cttgaaacat cagcgagata cggcgttttt cgggcggcat
      841 acgggtaatg ttttctccgt tcagccatat ttccccgccg tccggccgga caatccccgc
      901 aattatattc agcagggtgg attttccgca gcccgaccgc cccaaaacgg cgagtatttt
      961 gccgcgcccg acagtcaggc agatgttgtc ggcgacggtt ttattgccga agcgtttgca
     1021 gagtccgttc agttcaagca tggcgcatcc tataaacgta tgccgtccag ccactcggac
     1081 agcggatggg cggcatcaat cagaccgtaa cggcaaaacg cgtccagcgt aaccagttgc
     1141 gcgtcgtgca tcatgtttcc cgacaacatg gcatccaaca gaccgccgat gtccattttc
     1201 tcaaaacccg ccacttcgcc atcctgattt tcaggcagga aggtttcggg caggacggca
     1261 tcgaatacat acaggatttc attgtgtaca ccccggctga cggagcgcag gctgtgcagc
     1321 tgcgataccg ggcggatgag cggaagcagc gttttatcca aaccggcttc ttcgctgctt
     1381 tcgcgacaca cggcttcaga cggcatttcg ccgccggaaa caccgccggc ggcagtattg
     1441 tcgagtttgt tgggatcgac tgctttgtgc ggactgcgcc tgcctatcca gaaatgccat
     1501 cggccgtccg attcggtcag accgttgaga tggacggcgc ggctgagcag tccgaaagga
     1561 cggaaagcgg cgcgttcgag cgtgaacaag gggttgccgc cgccgtcggt caggtcgaaa
     1621 cactcgttgc gccagccgtc caacagcccc gcacagtgcc aaccgagggc gaggtgctgt
     1681 aagcgtccgc ccatatcagg ccagccgtcc gcattcagaa aaatgccgtc tgaagactcc
     1741 gagcagcctg cctcccagtc ttttttgacg cgttccaccc attccggcga caggttgccc
     1801 aaaggcagac cgttcagata cagcgttttc cagcaacttt ctgcaccgta acttgctttt
     1861 gcccactcga acagagcatc aaggtcttgt ttgctgacgg attcggtaaa acggacggtc
     1921 ggcatggttg gctttcttga agacaatgcg gaagattgta gccaaactgc ctgaatttgc
     1981 aaccgcaaac agacggcggt ctgccgttcc gatattgcgg ccggaaggaa aatccggcaa
     2041 aaaacggagg cggcaggaaa caggaaacag gaaacaggca aaacaaaacc cctcggcatt
     2101 gaaccgaggg gtttgatatt tggttgcggg agcaggattc gaacctacga ccttcgggtt
     2161 atgagcccga cgagctacca tgctgctcca tcccgcgtca gaagatgaaa ctatacggca
     2221 gattttttat gttgtcaaac attacttccg cgaaaaatca taaaattttg ccgtccggcg
     2281 gtttatgcct gatttgaaat attattttct ttacaaaagt tcatgttcgt gatttaattt
     2341 tggttaacat tgaaacaggg gtgctgcctg atgtttaggc ggctgagaaa taccctttac
     2401 acccgatcgg gataatacct gcgtggggag ttttcacgga ttctgctttt cagacggcat
     2461 tggttttcaa atgccgtctg aaaacgcaaa acgctcctgt ttctttaatt ctaaacgaga
     2521 aaacaggagc atttttttat gactacgcca aaaaaaaccg ccaaaacttc cggcaacgaa
     2581 gcgcgcgagc ttgccgactt gagcgaagac atcggcatct gctttaaata tccgaactcg
     2641 gaacgcgtgt atctgcaagg cagccgcgac gacatccgcg tgcctttgcg cgaaatccgt
     2701 caggacgaca cctacacggc gcaaggtacg gaagccaatc cgccgattcc cgtctacgac
     2761 accagcggcg tgtacggcga cccggcggcg catatcgacc tgaaacaagg tctgccgcac
     2821 atccgcaccg cgtggctgga cgaacgcggc gataccgaaa tcctgcccaa gctctccagc
     2881 gaatacggca tcgaacgcgc acacgatccg aaaaccgccc atctgcgttt caaccaaatc
     2941 acccgcccgc gccgcgcgaa aagcggcagc aacgtaaccc agcttcacta cgcgcgccaa
     3001 ggcattatca cgcccgaaat ggagtttgtc gccatacgcg aacgtttaaa attagacgaa
     3061 ttgtcccaaa aaccggaata cgccaaactc ttggaacagc acgcggggca aagtttcggt
     3121 gcgaacatcc cgacccatcc cgaccaaatc acgcccgaat tcgtgcgcca agaaatcgcc
     3181 gccggacgcg cgattattcc cgccaacatc aaccaccccg aactcgaacc gatgattatc
     3241 ggccgcaact ttcgtgtcaa aatcaacggc aacttgggca actccgccgt cacctccagc
     3301 ctgaccgaag aagtcgaaaa aatggtgtgg tcgctgcgtt ggggcgcgga cacgattatg
     3361 gatttgtcca ccggcgcgca catccatgaa acgcgcgaat ggattatccg caacgcgccc
     3421 gtccccatcg gcaccgtgcc gatttaccaa gcgttggaaa aaaccggcgg catcgccgaa
     3481 gatttgactt gggatttgtt ccgcgacacc ctcatcgaac aggcggagca aggcgtggac
     3541 tatttcacca tacacgcggg cgtgttgctg cgttatgtgc cgatgaccgc caaccgcctc
     3601 accggcatcg tctcgcgcgg cggttcgatt atggcgaaat ggtgtttggc acatcatcgg
     3661 gaaaatttcc tctacacgca tttcgacgaa atctgcgaaa ttatgaaagc gtatgacgta
     3721 tcgttcagcc tcggcgacgg cctgcgcccc ggctgcattg ccgatgccaa cgacgaatcc
     3781 caattcgccg aactgcacac cttgggcgaa ttgaccgata aagcgtggaa acatgacgta
     3841 caagtcatga tcgaaggccc cggccatgtg ccgctgcaac gcgtcaaaga aaacatgacc
     3901 gaagagctgc aacactgctt tgaagcacct ttttacacgc tcggcccgct cgttaccgac
     3961 atcgcacccg gctacgacca catcacctcg ggcataggcg cggccaatat cggctggtac
     4021 ggcacggcga tgctttgtta cgttaccccg aaagagcatt tggggctgcc cgacaaagaa
     4081 gacgtgcgca ccggcatcat cacctacaaa ctcgccgccc acgccgccga tctcgccaaa
     4141 ggctggccgg gcgcacaatt acgtgacaac gccctgagca aagcgcgttt cgagttccgc
     4201 tggcgcgacc aatttcgctt aagcctcgac cctgaacgtg ccgagagctt ccacgacgaa
     4261 accctgcctg ccgaaggcgc gaaaatcgcc cacttctgct cgatgtgcgg ccccaaattc
     4321 tgctcgatga aaatcacgca ggaagtgcgc gactacgccg acaagcaaaa agcccaacgg
     4381 cagggcatgg aggaaaaagc ggtcgagttc gtcaaaaaag gcgcgaagat ttacagttaa
     4441 acgtcaagca aaaaatgccg tctgaaaacc ggaaaaaagg cttcagacgg cattctttcg
     4501 cttgt
//
LOCUS       DAWIVB010000161         4364 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWIVB010000161 DAWIVB010000000
VERSION     DAWIVB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.161"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(69..2045)
                     /locus_tag="WIX60_001754"
     CDS             complement(69..2045)
                     /locus_tag="WIX60_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ5305984.1"
                     /translation="MNEFSLAPIVIVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGITMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WIX60_001755"
     CDS             2194..3033
                     /locus_tag="WIX60_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ5305985.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GETVIRDISALLGKPFVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARTRYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPTFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3232..4263
                     /gene="gap"
                     /locus_tag="WIX60_001756"
     CDS             3232..4263
                     /gene="gap"
                     /locus_tag="WIX60_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ5305986.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAAHDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1111 a         1457 c         1013 g          783 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca ttgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaat gccgacctgc aaaccgccca gaccgaacac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaatcacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgcattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga aaccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc tttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcac gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acgcccgaca caccgaaaaa accgacgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccagtcgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa ccctcaacaa aggaacgaac catgggcatc
     3241 aaagtcgcca tcaacggcta cggacgcatc ggccgccagg ttttgcgcgc catctacgat
     3301 tatcagattc aagaccaact ccaaatcgtc gccgtcaacg ccagcggcag ccttgaaacc
     3361 aacgcccatc tgaccaaatt cgacaccgta cacggacgct ttgaagccga cgtatcccac
     3421 gacggcggca acctcatcgt caacggcgac aaaatcccct tcttctcgac ccgcaaccct
     3481 gccgaactgc cgtggaaaga actcggtgtc gatttggtca tggaatgcac cggcgcgttc
     3541 accagcaaag aaaaagccaa agttcatctg gaaagcggcg caaaaaaagt cctcatttcc
     3601 gcaccgggcg gcgacgatgt cgatgcaacc gtcgtgtacg gcgtgaacga cagcgtcctg
     3661 accgccgaca tgaccgtcgt ttccaacgct tcctgcacca ccaactgcct ctcgccggtt
     3721 gccaaagtgt tgagcgaaag cgtcggcatc gtcaaaggcg cgatgaccac catccacgcg
     3781 ctgaccaacg accaaaccgt taccgacgtg cgccacaaag acctgcgccg cgcccgcagc
     3841 ggcgtggaaa acatgattcc gaccaaaacc ggcgcggcaa aagccgtcgg tttggtactg
     3901 cccgaattga aaggcaggct cgacgggctt gccatccgcg tgccgaccgt caacgtatca
     3961 ttggtagatt tgagcttcca agccgcgcac gacacgacag tcgaagaaat caacgcactg
     4021 atgaaagccg cctcggaagc aggcgcactc aaaggcgttt tgggctacaa caccctgccc
     4081 ttggtttcca tggacttcaa ccacaccacc gaagccagcc acttcgacgc aacgctgacc
     4141 aaagtcgttg acggcaacat gattaaagtc ttcgcatggt atgacaacga atggggattc
     4201 agctgtcaaa tgctgaacac tgcacgccgc atgttcggac ttgaagtgcg cccgctcaaa
     4261 taagcaataa accgtcaaac aaaatgccgt ctgaaacccg atgttttcaa gttccagacg
     4321 gcatttttca ccgtgatttt acccggctgt cgtcatttcc aatt
//
LOCUS       DAWIVB010000162         4357 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWIVB010000162 DAWIVB010000000
VERSION     DAWIVB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.162"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            123..1973
                     /locus_tag="WIX60_001757"
     CDS             123..1973
                     /locus_tag="WIX60_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ5305987.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPERKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVRRMAEDGAVKRALVLFQNSQ
                     SAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            2262..2474
                     /locus_tag="WIX60_001758"
     CDS             2262..2474
                     /locus_tag="WIX60_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ5305988.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2606..3052
                     /locus_tag="WIX60_001759"
     CDS             2606..3052
                     /locus_tag="WIX60_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ5305989.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VEAAVAETGAAGMADMGKVMGLLKTRLAGKADMGEVNKILKAVLTA"
     gene            3073..3222
                     /locus_tag="WIX60_001760"
     CDS             3073..3222
                     /locus_tag="WIX60_001760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305990.1"
                     /translation="MPSEARIAGSDGIFNIPISNRQGQHGFDTPKRVLPINRFRLRPI
                     GQNAV"
     gene            complement(3251..3643)
                     /locus_tag="WIX60_001761"
     CDS             complement(3251..3643)
                     /locus_tag="WIX60_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ5305991.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3715..4320)
                     /locus_tag="WIX60_001762"
     CDS             complement(3715..4320)
                     /locus_tag="WIX60_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ5305992.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         1059 a         1232 c         1205 g          861 t
ORIGIN      
        1 atgtcggaaa cattccaaag gcggtgcagt ttcggcatat aattcgggca aacgcctgtt
       61 cagacggcat tttgtctttt ccaaccctga ccgttcaggg ttccgattct taaggaaatc
      121 cgatgtacct accctctatg aagcattccc tgccgctgct ggcggccctg gtgcttgccg
      181 cgtgttcttc gacaaacaca ctgccagccg gcaagacccc ggcagacaat atagaaactg
      241 ccgacctttc ggcaagcgtt cccacccgcc ctgccgaacc cgaaagaaaa acgctggcag
      301 attacggcgg ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg
      361 ccgcctattt ggaaaacgcc ggcgacagcg cgatggcgga aaatgtccgc aacgagtggc
      421 tgaagtcttt gggcgcacgc agacagtgga cgctgtttgc acaggaatac gccaaactcg
      481 aaccggcagg gcgcgcccaa gaagtcgaat gctacgccga ttcgagccgc aacgactata
      541 cgcgtgccgc tgaactggtc aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt
      601 tggaacaggc agccgcatcc ggcttgttgg acggcaacga cgcctggagg cgcgtgcgcg
      661 gactgctggc cggccgccaa accacagacg cacgcaacct tgccgccgca ttgggcagcc
      721 cgtttgacgg cggtacacaa ggttcgcgcg aatatgccct gttgaacgtc atcggcaaag
      781 aagcacgcaa atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc
      841 tcgaacaacg cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa aacctcaatg
      901 tgcctgccgc cttggactat tacggcaagg ttgccgaccg ccgccaactg accgacgacc
      961 aaatcgagtg gtacgcccgc gccgccttgc gcgcccgacg ttgggacgag ctggcctccg
     1021 ttatctcgca tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac
     1081 gcagccgcgc cgcaacgggc aacacgcaag aggcggaaaa actttacaaa caggcggcag
     1141 cgacgggcag gaatttttat gcggtgctgg caggggaaga attgggtcgg aaaatcgata
     1201 cgcgcaacaa tgtgcccgat gccggcaaaa acagcgtccg ccgcatggcg gaagacggtg
     1261 cagtcaaacg cgcactggta ctgttccaaa acagccaatc tgccggtgat gcaaaaatgc
     1321 gccgtcaggc tcaggcggaa tggcgttttg ccacacgcgg ctttgacgaa gacaagctgc
     1381 tgaccgccgc gcaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg
     1441 aacgcaccga ccgcaaactc aactacacct tgcgctatat ttcgccgttt aaagacacgg
     1501 taatccgcca cgcgcaaaat gttaatgtcg atccggcttg ggtttatggg ctgattcgtc
     1561 aggaaagccg cttcgttata ggcgcgcaat cccgcgtagg cgcgcagggg ctgatgcagg
     1621 ttatgcctgc caccgcgcgc gaaatcgccg gcaaaatcgg tatggatgcc gcacaacttt
     1681 acaccgccga cggcaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc
     1741 tgcaaaacaa cgaagtcctc gccaccgcag gctataacgc cggtcccggc agggcgcgcc
     1801 gatggcaggc ggacacgccc ctcgaaggcg cggtatatgc cgaaaccatc ccgttttccg
     1861 aaacgcgcga ctatgtcaaa aaagtgatgg ccaatgccgc ctactacgcc gccctcttcg
     1921 gcgcgccgca catcccgctc aaacagcgta tgggcattgt tcctgcacgc tgacgtaccg
     1981 atgccgtctg aaacccgccc ggtctttcag acggcatttt tatcccgaac ggcattgacg
     2041 gcgaaccata aatataagac aatccgaaaa ttgtttttcc tgctttttca agcagcttga
     2101 cacggcacaa gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc
     2161 gcaaggcaca gcgatacggt aaactttcaa caccgtctgc cctacccttt ccaccgatat
     2221 gatgggcaga tgaaacaacc gaatttatta aaggaaataa aatgcctgca atccgcgtaa
     2281 aagagaatga accatttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg
     2341 gcctgctgac cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac
     2401 gcaaaaaagc ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa caactgccgc
     2461 ccaaaatgta ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca
     2521 ccgcaagacc tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtttc
     2581 catccgacat cccagcgagg acatcatgag cctgaaaatc cgccttaccg aagacatgaa
     2641 aaccgcgatg cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc
     2701 cgtcaaacag tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat
     2761 cctgaccaaa atggtcaaac agcgaaaaga cagcgcgaaa atctacactg aagccggccg
     2821 tcaggatttg gcagacaaag aaaacgccga aatcgaggta ctgcaccgct accttcccca
     2881 aatgctttcc gccggcgaaa tccgtaccga ggtcgaagct gccgttgccg aaaccggcgc
     2941 ggcaggtatg gcggatatgg gtaaagtcat ggggctgctg aaaacccgcc tcgcaggtaa
     3001 agccgacatg ggcgaagtca acaaaatcct gaaagccgtg ctgaccgcct gattgcccga
     3061 atatcggaca aaatgccgtc tgaagcccgt atcgcaggtt cagacggcat tttcaatatc
     3121 ccaatatcga atcggcaggg gcaacacggt tttgatacgc cgaaacgggt tttgccgata
     3181 aacagattcc gtttgcgccc catcggacaa aatgccgtct gaaacacgat tccgttcaga
     3241 cggcatagat ttatttgacc aatttcaagc cttttttggc gggtcggggc gcggtttcgg
     3301 cagaggtgtt ttcaggcggc gtatcggggc ggtacgcttc caactcaaac cccatacctt
     3361 ctccggtctc ccgtgcgaaa aggctgagga cgtgtccgac aggtatccat atatcgtgcg
     3421 cctgtccgcc gaagcgggcg gaaaagctga tccaatcgtt gtcgatttga aggttttgcg
     3481 tggcggtcgc gccgatgttg agcataattt cgttgtcgcg gacgtactgc atggggacgc
     3541 gcgtgtgttc gttgacccag acaaggatgt gcggtgtgag gctgttgtcg ctgcaccatt
     3601 cgcagagggc gcggaggatg tagggtttgg tggaagtggg cataatgggt tccgtgttgt
     3661 acgccaaaat aggaaaatgc ctgcaaaacg gtgggttttg caagcatttc ggacttattt
     3721 gcgcatggct ttttcggcgg gtgtcagtgc ttcgataaag gcttcgcgct ggaagatgcg
     3781 ctcggcgtat ttgagcagcg gcgcggcact tttgcccagt ttgacatcgt agtggtcgag
     3841 ccgccacagc agcggagcaa gggcgacatc aatcatagaa aaatcttcgc cgaggatgta
     3901 tttgcttttg ctgaacgaag gggcaagcat ggtcagaccg ttgccgatgg cttcgcgcgc
     3961 ttttgcctgt tccttgttgg tggcggcggg gttttctaac acttggacgt ggttgaacaa
     4021 ttctttttcc atacggtaca gcaccagccg gccccgaccg cgcataacgg gatcgccggg
     4081 catcagctgc ggatggggga agcgttcgtc aatgtattcg ttgatgatat tggactcgtg
     4141 cagcaccaaa tcgcgctcga ccagcacggg aacttggtta tacggattca tgacggcgag
     4201 gtcttcgggt ttgttgtaaa tatcgacgtc tttgatttca aaatccatac ctttttcgta
     4261 caaaacgaag cggcagcggt ggctgaaggg gcaggtaatg ccggaataga gggtcatcat
     4321 aataattgtc gctcctgtgt gatgcctgca aaacggc
//
LOCUS       DAWIVB010000163         4301 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWIVB010000163 DAWIVB010000000
VERSION     DAWIVB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.163"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            579..2306
                     /gene="ilvB"
                     /locus_tag="WIX60_001763"
     CDS             579..2306
                     /gene="ilvB"
                     /locus_tag="WIX60_001763"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ5305993.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            2317..2808
                     /gene="ilvN"
                     /locus_tag="WIX60_001764"
     CDS             2317..2808
                     /gene="ilvN"
                     /locus_tag="WIX60_001764"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ5305994.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2875..3168
                     /locus_tag="WIX60_001765"
     CDS             2875..3168
                     /locus_tag="WIX60_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ5305995.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3248..4261
                     /gene="ilvC"
                     /locus_tag="WIX60_001766"
     CDS             3248..4261
                     /gene="ilvC"
                     /locus_tag="WIX60_001766"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ5305996.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1119 a         1031 c         1081 g         1070 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatac aatcaatata ttaagtcgtt
       61 gttagccctc ttggttctgt caatgccgtc caatacagtc cggtgcaatg agccattttc
      121 aggaagtatg gttgggaata tttttgatat atcaaaaata aaatcaagtt caatcaatat
      181 cccgagtctt tattgggatg aaaccctgta aattttacaa ttcttgatat cgaagaaggg
      241 tttttcacat ctgcccctcc tacacaaact gtcaacaatt tccatctttc aaaaaaggta
      301 ctcctgattt tattctttta aaatcaatca ataatttctc tgctttcaaa aaatattaaa
      361 agatgaaaga tttttcttga ttgaactgtc ggtttgcgtt atcgtttcta ctctttttca
      421 caaactctgt gttcctttcc aattgattgg ataatccgtc tgttgccgtg ttcccccaat
      481 gcgcgtaccc taaatcgctg cttggtggaa ttgcattaca ggtgctgtgg caaagcggtt
      541 tgtccctatt ggtttgaaac cgtataaaag aggtcgttat gcagttatca ggcgcgcaaa
      601 tcatagtgca gagtctcaaa gccgaaggtg tcgagtacgt tttcggttat cccggcggtg
      661 cggttatcga aatctacgat gcccttttcc aactcaataa attcaaacac attcttaccc
      721 gccatgagca ggcggcagta cacgcggcag atgcgtatgc gcgcgtcagc ggtaaggtgg
      781 gcgtggcatt ggttacatcc ggcccgggcg ttaccaatgc actgaccggt attgctactg
      841 cctatacgga ttcgattccg atggtggtca tcagcgggca ggtaggcaat tccctgattg
      901 gtacggatgc gttccaagaa gttgatacgg tgggtattac ccgtccgtgc gtcaaacaca
      961 atttcctggt tacggacatc aatgagttgg cggaaaccat taaaaaggcg ttccaaattg
     1021 ccgcaagcgg ccgaccgggg cccgtggtgg ttgatgtccc gaaagatgtt acgcaggcga
     1081 tggcgaaatt cagctatcct caggaagaca tttttatccg ttcgtatcaa ccggttgttc
     1141 aaggacatat cggtcagatt aaaaaggccg tgcagatgtt ggcatctgcc aaacgtccgg
     1201 tcgtctattt tggcggcggc gtggtgttgg gtaatgcttc tgaagagctg acccgatttg
     1261 tccgaatgac gggtgctccg tgcacgggta cgctgatggg cttgggcgct tatccttccg
     1321 gcgaccgcca attcctcggt atgctcggta tgcacggtac ttacgaggca aaccttgcca
     1381 tgcagaatgc ggatgtcgtt cttgccgtag gtgcgcgttt tgacgaccgt gtggtttccg
     1441 taccgtccaa atttttcgag aaggcgaaga aggttatcca tatcgatgtc gatccttcca
     1501 gcatcgccaa acgcgtgaag gtggacattc cgattgtcgg cgacgtgaaa aacgttttgt
     1561 ccgagatggt tgcgctgtgg caaaaacaag agtccgtgcc gtctgaagat gctttgggca
     1621 aatggtggaa aaccatagag gaatggcgtt cccgagattg cttgtggttt gacaacggca
     1681 gcgaaattat caagccacaa tatgtgattc agaagcttgc cgagattacc ggcaattcgg
     1741 caatcatcac atcggatgta gggcagcatc aaatgtttgc ggctcaatat tatcccttcg
     1801 aacgtccgcg ccaatggctc aattccggcg gtttgggtac gatgggcgtg ggtctgcctt
     1861 atgcgattgg tgcaaaactt gccgccccgg atcaagacgt attctgtatc accggcgacg
     1921 gctcgatcca gatgaacatc caagagctgt ccacctgttt ccaatatcgg attccggtta
     1981 acgtcattac gctgaacaac ggttatctcg gtatggtacg ccagtggcag gaaatatatt
     2041 acggcggacg agagtcggaa acctatttcg attctttgcc cgatttcgtc aaacttgccg
     2101 aggcatacgg ccatatcggt atccgcgtgg acaagaagtc tgatgtggaa ggtgcgttgt
     2161 tggaagcatt gaaccaaaaa gacaggctgg tgtttatcga cttcctgacc gaccagaaac
     2221 agaatgtgat gcccatggtc ggcaacggca aaggtttgga cgaaatggta cttccgccgc
     2281 atatgcgtgc ggacggaaag gcgtaaggag agacaaatgc gacatatctt atctgttctg
     2341 attgaaaacg aatcaggtgc gatgagccgc gtggtcggtt tgttctctgc acgcgattac
     2401 aatatcgatt ctttggcggt tgcgccgacc gaagacaaaa ccctgtcacg gatgaccatc
     2461 gttacccacg gcgacgagca ggtcatcgaa caaattacca agcaactcaa taaattgatt
     2521 gaagtgatta aagtggtcga tttgaatgaa agccgttttg tcgaacgcga actgatgttg
     2581 gtaaaagtcc gtgccgccgg caaagaccgc gacgaatttt tacgcttgac cgaaatctac
     2641 cggggcagca tcatcgacgt aaccgaccgc agctatacga ttgaaattac cggctcgaca
     2701 gacaagctgg attccttttt ggaaacggtc ggacgcgccc aaattttgga aaccgtacgc
     2761 acaggcgcgg ccggcatcgg tcgcggtgag cgtattttga aaatttaacg ccgtaacctt
     2821 tcagacggca cggtatttga atgtcgtctg aaaaacgaac ggcaggagag atttatgtcg
     2881 aacattaaaa ttgtcgcact ggttaccgtc aaaccggaat acacagaaac actggcagca
     2941 cagtttaaag aactggtcaa agccagccgt gcagaagagg gcaacatcag ctacgatctc
     3001 catcaggaaa tcggcaaacc gaaccgtttt gttttcgtgg aaaattggaa atctcaagca
     3061 gctattgacg aacataacgc cagcgcgcat ttccaagcct tcgtccagtc cgtcgacggc
     3121 aaaaccgaag cgttggaaat cgtattgatg aatgaagttg ccgattaagc ggcacactct
     3181 gtttaacccg tccgaagccg ctcaacattt taaggcttcg acaaccattt accttaaagg
     3241 aaatcaaatg caagtctatt acgataaaga tgccgatctg tccctaatca aaggcaaaac
     3301 cgttgccatc atcggttacg gttcgcaagg tcatgcccat gccgccaacc tgaaagattc
     3361 gggtgtaaac gtggtgattg gtctgcgcca aggttcttct tggaaaaaag ccgaagcagc
     3421 cggtcatgtc gtcaaaaccg ttgctgaagc gaccaaagaa gccgatgtcg ttatgctgct
     3481 gctgcctgac gaaaccatgc ctgccgtcta tcacgccgaa gttacagcca atttgaaaga
     3541 aggcgcaacg ctggcatttg cacacggctt caacgtgcac tacaaccaaa tcgttccgcg
     3601 tgccgacttg gacgtgatta tggttgcccc caaaggtccg ggccataccg tacgcagtga
     3661 atacaaacgc ggcggcggcg tgccttctct gattgccgtt taccaagaca attccggcaa
     3721 agccaaagac atcgccctgt cttatgcggc tgccaacggc ggcaccaaag gcggtgtgat
     3781 tgaaaccact ttccgcgaag aaaccgaaac cgatctgttc ggcgaacaag ccgtattgtg
     3841 cggcggcgtg gtcgagttga tcaaggcggg ttttgaaacc ctgaccgaag ccggttacgc
     3901 gcctgaaatg gcttacttcg aatgtctgca cgaaatgaaa ctgatcgttg acctgatttt
     3961 cgaaggcggt attgccaata tgaactactc catttccaac aatgcggagt acggcgaata
     4021 cgttaccggc cctgaagtgg tcaatgcttc cagcaaagaa gccatgcgca atgccctgaa
     4081 acgcattcaa accggcgaat acgcaaaaat gtttatccaa gagggtaatg tcaactatgc
     4141 gtctatgact gcccgccgcc gtctgaatgc cgaccaccaa gttgaaaaag tcggcgcaca
     4201 actgcgtgcc atgatgcctt ggattactgc caacaaattg gttgaccaag acaaaaactg
     4261 attgttttca aacgggacta cctacacatc gtgtaggcag t
//
LOCUS       DAWIVB010000164         4243 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWIVB010000164 DAWIVB010000000
VERSION     DAWIVB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.164"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(257..1660)
                     /locus_tag="WIX60_001767"
     CDS             complement(257..1660)
                     /locus_tag="WIX60_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ5305997.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(1729..3156)
                     /locus_tag="WIX60_001768"
     CDS             complement(1729..3156)
                     /locus_tag="WIX60_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ5305998.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3392..3745
                     /locus_tag="WIX60_001769"
     CDS             3392..3745
                     /locus_tag="WIX60_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5305999.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            3742..3963
                     /locus_tag="WIX60_001770"
     CDS             3742..3963
                     /locus_tag="WIX60_001770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306000.1"
                     /translation="MKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFSDG
                     EYRYVDVLQPNHSDIIRYSGKDFSAKSNI"
     gene            4034..>4243
                     /locus_tag="WIX60_001771"
     CDS             4034..>4243
                     /locus_tag="WIX60_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306001.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILR"
BASE COUNT          998 a         1119 c         1039 g         1087 t
ORIGIN      
        1 gtttgggcga atattaagcg gtatctgcga accgtattgt ctgattacgc ccgatttaac
       61 gatgcactac tgtcctattt tgattttaat tgaatataca tttgaattaa ttcgcactta
      121 atttaaatgt gtttttaact gtgctttatt taaaggcaat gagaatgtga aaatatcgga
      181 tcaatcccaa agcagcctgc actttcgaaa cggggtgcag gctgctttgg gaatttcata
      241 accgtttcag cctgctttat tccgcaaata ccgtttccaa ccctaacccg ctctctttca
      301 ccaagcgcaa ataagccagc atgaatttat accgtgcttg agccagtttc tgttctgctt
      361 gggcgacttc ctgccgcgcc cgtattactt ccagccggtt gcggatgccg tattgttggc
      421 cggtttcggt cgatttcagt ttcaaacggc tgctttccaa aacccgttct tgcgccatga
      481 tttggtaacg cgccgcaccg ctttcggtat aagcctggcg tacggcgagt ttgatgtgcc
      541 gctcggttgc ggtcagctgt gcttcggcgg ccccgtattg cgcttcggct tcatggattt
      601 tgcccgacaa ttctccgccg gtataaagcg gcaaattcaa ctgtacgccg acgctcatcc
      661 ctttgccccg atagtggtag tcattattct gcgcagatga agtgtagagg ttattctgat
      721 agccgacatg ggcagaaacg gtgggatagc ggctgttctg tgctgcccga agcgcctgtc
      781 cgctgctttg cagggcaagc tgctgcatcc ggtattcatg attgttggat aaggcaatgc
      841 gctgccattc atccagactg taacgttcca gcttgggcag atagcgtgcc aacaggttgg
      901 cggtatctat ggcctcgatt tgtttgctat ccaggtcggt gtagtcgttc aactggtttt
      961 cataggtttg tttctcagcc aatacggcga tttcttgggc cagggcattg tcgtaaccgg
     1021 ctttggcttc gtgaatatcc agcgcggtgg cagcaccttt attgaataaa gcctgcgcct
     1081 gccttacctg ctgggcataa gcctcttttt ccgccgcatg ggcggcaacg gtgtctcggc
     1141 tgagtaaaac gttgaaataa ctttcggcaa ctttcaacag caattcttcg cgtgccgcat
     1201 cgaaacgctg ttctgcagcc tgcgtatcga acctgctttg gcggtattgt gcaaatttgg
     1261 cagcgtcaaa taaggtttgt cccacctgca cgctccatcc ctgtgtttcg cgggtggaag
     1321 aaatcgatgg cggctggcgc tggtagctgg cattggcgga tacatgggga aggaatgcgg
     1381 ccttggcttg ttgttgccgt gcgcgcactg catcacgctg gtaatgggac gcttgaaaat
     1441 cagccgaatg ttgctgcgcc gcccgccatg cttcaggcag cgtaaaagcc gaaacggatg
     1501 gggaaaggga tagtggcaag gtaaaaagtg aaacgggtag gatatatttg gaaaaatagg
     1561 atttcatagc cgaaaatagt tcatgttgca aatagggcgt cagtgtcagg caaacggaaa
     1621 taccgtaatc ttgcattatc attagattga gcaatgtcat ccgggcaatg gtttcaggca
     1681 gtctgcatgt ccgaaccggc ggataacaaa tgcccagtac ggatccgcct atcgctccct
     1741 aaagctttcg tccaatttgg tttgcagcgg gcttaacaga taatccagca cccgccgttt
     1801 acccgtttta atctccgccg tgacattcat gcccgccgtc agattcactg ctttgccgtc
     1861 aatattcaag gtatgtttgt ccagcgacac caccgccgta taaaccaagc ccaactgttc
     1921 gtggcttacc gcatcatggc tgacactttt caccttgccc gtcagataac cgtagcgcgt
     1981 atagggaaag ctctcaatct tcaccaccgc atcctgtccc tgttccacaa aaccgatgtc
     2041 tttgttcaat accaaaactt ccacgtccat tttgtcgtca tcgggcgcaa tcaccatcat
     2101 tttttgggca gcctgcacca caccgcccac cgtataggta gccaattcct gcaccgtgcc
     2161 gtccgcaggc gactgtattg tcatcagctg ctgccgctgc tttgccttat ccgtttggcc
     2221 gcggtattgg tcaatctgtt cgtttgcctg gcgcagcgca tccagcgtat cgcgtttcag
     2281 gttctgcgta ttcagcaccc gattctgctc cgcctgtgca atggccgcct gaatctgcct
     2341 catctgaccg cgcgtacttt ccaaatcgtt ccaattgctg accgatttgc tctgctgctc
     2401 caaaaacgca tgttccgaaa taaaattgtc ggcccgcaaa cggcggtagt ctgctgtttt
     2461 ctgctgctcg atcgccccca ccgaaaccag cttctgctcc tgcgccttgg ccgactgcaa
     2521 ttccgcctga tggccgcgca aagccgactg caattgcgca tcctgcgccg cccatgcctg
     2581 atactggtgc tgcgccaaca cctgcgccga ttgcacatcg gcatcggaga gacctaaaga
     2641 ccgtgcttgc gccatatcga tatgcggcac ggtacggctt tccaatgccg ccaataccgc
     2701 ttcataacgc agtttggaca attgggcagc ctgcaaagcc tgctccgact gcaccacatc
     2761 gctgtctgtt cccacagcct ccagttccgc cagcgtttct ccctgtttca catgctgccc
     2821 gtcgcgcaca tgtaccgcct taaccaccgc cgtttccagc ggctggatgg ttttgctgcg
     2881 cccgcccgac accgttttgc ccgaagccgc cgccacaata tcgattttgc cgaaccagga
     2941 ccacaacaaa gccaaaagcg caaacgccat aataaaacgc gccgcccatt tcggagcggc
     3001 agagaccggc gtatcggtca gttccaaatg cgcgggcaaa aacgcctgtt cttccgccgt
     3061 gcgtttgggc ggtttcaact ggtcgcgcac cgcccaaaca ttgcgccata cagtaatgta
     3121 tcgagaaaga aaggatttca gggcggagaa aaacataacg ggtataacct tggcaatata
     3181 gaaacaggaa acaatataaa tatgtaaagg aattttaacg gaaagcgcgg cagctgttaa
     3241 gggaaaggcg ggaatattga caaaaaatac ccaagtcgtt acaaatattc attattttac
     3301 tgcgtaacgc aacgctgaag cgcaggctgc ttttgagatg cggcaaggtt cggcaaaaag
     3361 cagcctgcac atttaaccac aggaacaacc catgtttacc acaaacgatt tacgccattt
     3421 cctagaaggt ttggccatcc tattctcaat cggctattgg ggcaccatgc tgctgttgct
     3481 ttggtttctc gtccgctttg cctataaaaa gcccaaacgg aaccccggca aaatcctttc
     3541 aggcagcctg accgccgccg ctatcctgat gctgtttgtt tggattattc ccaaacaatt
     3601 cggcccgatc aaagaagaaa tacaggcaca agaagagtgg gacagaaaat acaaagaagc
     3661 cgaagccgtg tttaacgaac aatgcaaaac ggcgggggaa agatttacca gacggcggac
     3721 aatgtggaag ggattatgct gttgaaggta gtacctgagc gtaccgtttc ggcagatgca
     3781 aaaaccagag acccgatgtg ggacaatgcg gctttacaga ccagcgaagg cgtaaatttt
     3841 attgctcgtt tcctaggatt ttttagcgat ggggaatacc gctatgtgga tgtcctgcaa
     3901 cccaaccatt ccgatattat tcggtattca ggtaaagatt tttccgctaa atcaaatatt
     3961 taatcatata caccccgccc gttatgcggt aacgttcgaa aacaatgtcg attccaagct
     4021 gcgcaggcac tgggtggcag gtgcgaccat acggattatc gaccgccaaa ctgacgaagt
     4081 gattgccaag aaaaccatct atgtctttga aaaaggcttg gacggcacgg gtggggcgag
     4141 aatgccgtgg aagtttgcta tcttgtgcaa taaagaaaga cttacttctt cagagccgtt
     4201 atcggatttt gttcttagcg ttttaaaacc ttatatattg cgt
//
LOCUS       DAWIVB010000165         4214 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWIVB010000165 DAWIVB010000000
VERSION     DAWIVB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.165"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(77..313)
                     /locus_tag="WIX60_001772"
                     /pseudo
     CDS             complement(77..313)
                     /locus_tag="WIX60_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            509..967
                     /locus_tag="WIX60_001773"
                     /pseudo
     CDS             509..967
                     /locus_tag="WIX60_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            867..1235
                     /locus_tag="WIX60_001774"
     CDS             867..1235
                     /locus_tag="WIX60_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ5306002.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1304..2842
                     /gene="lnt"
                     /locus_tag="WIX60_001775"
     CDS             1304..2842
                     /gene="lnt"
                     /locus_tag="WIX60_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ5306003.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASNNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMDMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILALAALILFIFR
                     NKEH"
     gene            3107..3970
                     /gene="rpoH"
                     /locus_tag="WIX60_001776"
     CDS             3107..3970
                     /gene="rpoH"
                     /locus_tag="WIX60_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ5306004.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNRIPMLTQEEETRLAERQHK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
BASE COUNT         1038 a         1300 c          966 g          910 t
ORIGIN      
        1 accttgccgt cgggcaggat gtaccacgcc aaatccccgc ctgaagcacg gtgtcggact
       61 gcatttctat tcggctatac gccttttcac gcaggctgta attgaatttc cacaaaccgc
      121 cgaacactgc cgacggacgc gcgaccagca tcaggaacgc cgcaatcgcc aaggcttccg
      181 ccgccctgtc caacacgttg gcaggagaaa ccagcagcaa aggcattccc aaacgtgcgg
      241 acaaagtggt cgaaaccacg ctcagaaaca acagtgcgcc acccggcagg aacagactgc
      301 ccatccaatc cacgctaacg attctccctt aaaattcatg aaattaaaat cgaaagactg
      361 taacacggta ttttcttttc acgaaacttt acttcacgtc cgccacagcg ttcagacggc
      421 attttcctgt ttcatgccgc ctgaaaccgc ccaaatccgc gttctcctat ataatgtcgg
      481 actttgcgcc gcatttcagg attctttaat ggcatttgcc tcactattta ccctgccgga
      541 cgacatcacc gccgttttgg acgatgttgc cctgatgacc aaagccgccg caaagaaaac
      601 cgccggcgtg gtcggcgacg atttggcacc caatgccaac cgggttacgg gcgtatcggc
      661 agaacgcgag ttgccgattg tttggtcgat ggcgaaaggt tctttttcca acaagccggt
      721 tttggtgcct gcggccctgc tgctttccgc cttcctgccg caactcatta cgccgctttt
      781 gacggcaggc ggcatttatc tgtgctttga aggcgtagaa aagctgctgc acaagtttct
      841 gcaccgacat gacgcgcatg ctcgtaatgg cggcaatcgg catcggcatg actgttttgg
      901 tgtacggaat cgttgccgtc atcgtcaaac tcgacgactt aggcatgctg ctgatgcgcc
      961 gcccacaaac cttttcgcgc agtttggggc aaatgctgac tgcctttatg ccttgcttta
     1021 tgcgcggttt gagcgtggtc ggcacgcttg ccatgtttct tatcggcggc gtactggtcg
     1081 cccacaacct cggccttctg cacgatttcc tgcacgcgca acactgggac gctggctggg
     1141 cggagtactt cgccaacctc gttgtcgggc tgctttccgg ttcgattgcc tgcgcccccg
     1201 ccttgccgct gatgaatcgt ttcggcaggc attgattcct ttttcaaata ccgatgccgt
     1261 ttgaaagatg ttcagacggt atcttccgaa cagacagatg aatatggttt ccaaactgga
     1321 caaatactgg cagcaccccg ccctctactg gcctttgctc atcctttttg ccgccgccac
     1381 cccctttacc ttcgcaccct actaccactt ttggctgatg cccttgattt tcggtgcctt
     1441 cgtccgcctc atcgaactgc gtccgcgttt tgctgtctct tccgcctacc tgttcggcct
     1501 gaccgcatac acgacacagt tctactggat acacaccgcc ctgcacgacg tttccggcct
     1561 gcccgacctc tatgccgtac cgctgacctt cctactcccc gcctaccttg ccctttatcc
     1621 ggcactgtgt ttctggctgt ggaaaaaatt taccctgcct cggggcataa aaatcggttt
     1681 ggtactgccc atcctgtgga cgctgaccga gtttgcccgc gaacgtttcc tgaccggatt
     1741 cggctggggc gcaatcggct actcccaaat caccccggac agcccgctcg ccggctttgc
     1801 cccattgggc ggcatccaca tggttacact ggcaaccgcc tttctcggtg tctggctggt
     1861 tttggcgagt aataacaccg cacgttcggg caaacgcctg cttccgatta tcctgattgc
     1921 cgccctgctt gccgcaggct acaccgcccg acaaaccgac ttcacccgcc ccgacggcag
     1981 ccgcagcacc gtcgccctgc ttcaaggcaa catcgaccaa accctcaaat ggcgtgaaga
     2041 ccaagttatc ccgaccatac agaaatatta cgaacaagtc ggcaaaacca ccgccgacat
     2101 cgtcatcctg cccgaaaccg ccatccccgt tatgcgccaa aacctgccgg aaaacatact
     2161 ggcgaaattt gccgaacagg cgcaaaacaa cggcagcgcg ctcgccgtcg gcatcagcca
     2221 atacacttcg gacggcaacg gttacgaaaa cgccgtcatc aacctgaccg gttatcagga
     2281 aaacaatcag gacggtatcc cctactacgc caaaaaccac ctcgtcccct tcggcgaata
     2341 caaaccgctg cctttcctga ccacgccgct ttacaaaatg atggatatgc ccctttccga
     2401 cttccgcaaa ggcggcggca agcaatccgc cctgctgatg aaaaaccaaa aaatcgcctt
     2461 caacatctgt tacgaagacg gattcggcga cgaactgatt gccgccgcaa aagatgccac
     2521 cctgcttgcc aatgccagca atatggcgtg gtacggaaaa tccaacgcca tgtaccagca
     2581 cctccaacaa tcgcaggcgc gggctatgga actcggacgc tatatggtcc gcgccaccaa
     2641 caccggcgca accgccatca tctcccccaa aggcaacatc atcgcccaag cccaacccga
     2701 tacggaaacc gtattggaag gacacatcaa aggctatgtc ggcgaaacgc cctatatgaa
     2761 aaccggcagt tcatggtggt tgatgggcat attggcccta gccgcactga ttcttttcat
     2821 cttccgaaac aaagaacact gatacctgca cagcccgtcc ggacgggctg tcttttttca
     2881 aaacggctat ttacatacaa ccacgtatgt aatataattc caaaatcgaa ttgagccatc
     2941 aggtttacaa aatcaatcaa acttgcctta caaaattttt cctgtctttc aaaccttagc
     3001 gcatatctcc tgccaaatat acaaaataca agccaagcag tgatatactg catcccaaca
     3061 cagcaaaccg ccgatacgca gtttcagccg aaaggaaatg tcccaaatga ataacgcttt
     3121 cgcattaccc gccatccaaa gcggcaacgg cagcctcgaa caatacatcc acaccgtcaa
     3181 cagaattccc atgctgaccc aagaggaaga aacacgcctt gccgaacgcc aacacaaagg
     3241 cgacctcaac gccgccaaac aactcatcct gtcccacctg cgcgtcgtcg tttccatcgc
     3301 gcgcggctat gacggctacg ggctgaatca ggcagacctg attcaggaag gcaatatcgg
     3361 actgatgaag gcggtcaaac gctacgagcc cggtagaggc gcgcgtctgt tttcatttgc
     3421 cgtacactgg attaaagccg aaatccacga gtttattttg agaaactggc gtttggttcg
     3481 cgttgccacc accaaaccgc aacgcaagct gtttttcaac ctgcgcagca tgcgtaaaaa
     3541 cctcaacgcc ctgtctccca aagaggcaca agacatcgcc gacgatttgg gtgtcaaact
     3601 ttccgaagtt ctggaaatgg aacagcgaat gacgggacac gacatcgcca tcatggcaga
     3661 caacagcgac gacgaggata gtttcgcccc catcgactgg cttgccgacc acgattccga
     3721 accgagccga caactatcga aacaggcgca ttacgccctg caaacagaag gtttacaaaa
     3781 cgctttggcg caactggatg acaggagccg ccgtattgtg gaaagccgct ggcttcaaga
     3841 cgacggcggg ctgacgctgc accagcttgc cgccgaatac ggcgtatctg ccgaacgcat
     3901 ccgccaaatc gaagcaaaag ccatgcagaa actgcgtggt ttcctgaccg aagaagccga
     3961 agcggtttga aatatacgca ataaaatacc cgacaaattg ttttgtcggg tattttattt
     4021 ggtaaacatc aatgcggttt gtgcgtcgtg ctgtacacga ttttatccgt ccacgccgtt
     4081 gcaattgccg aaatcaaaaa gcagacatgc accagacact gccgcatcag ctgcttttcc
     4141 gacagattgg cggaattgat atagtggatt aacaaaaatc aggacaaggc ggcgaagccg
     4201 cagacagtac aaat
//
LOCUS       DAWIVB010000166         4205 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWIVB010000166 DAWIVB010000000
VERSION     DAWIVB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.166"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(1..143)
                     /locus_tag="WIX60_001777"
                     /pseudo
     CDS             complement(1..143)
                     /locus_tag="WIX60_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(271..1521)
                     /locus_tag="WIX60_001778"
     CDS             complement(271..1521)
                     /locus_tag="WIX60_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ5306005.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     DAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVRKQVTALCNKYPVYGA"
     gene            complement(1588..1782)
                     /locus_tag="WIX60_001779"
     CDS             complement(1588..1782)
                     /locus_tag="WIX60_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306006.1"
                     /translation="MADKDVVGAGYLIDFAQTAENLPFNVLLLISLVLNKGNETLKTS
                     MLNKLPDNAKENLRIMGYLP"
     gene            complement(2003..3778)
                     /gene="ggt"
                     /locus_tag="WIX60_001780"
     CDS             complement(2003..3778)
                     /gene="ggt"
                     /locus_tag="WIX60_001780"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ5306007.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQNAISAPNLLNRFGSYELETGTTAVQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3946..4182)
                     /locus_tag="WIX60_001781"
     CDS             complement(3946..4182)
                     /locus_tag="WIX60_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ5306008.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIASRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
BASE COUNT         1050 a          986 c          998 g         1171 t
ORIGIN      
        1 atctctgagg taacgggttt ttgacggttc aggtatggtt cgatcggctg ccaatcaatc
       61 acccggtcca acttcaatag cgggaaacgg tcgatgtgtt tggcaattat ggcttgtgcg
      121 gtttgccgga agaaggtgct catgagaaat cccctaaatg tcttggtggg aatttagggg
      181 attttgggga tttttgcaaa ggtttccgcc tgaaacatta tgagatttca ggcggcattg
      241 gattgcttgg cggaatattt ttaaaaaggc ttacgcgccg taaacggggt atttattgca
      301 caaagcagtt acttgtttgc ggactttggc gaggttggct tcgtcttcgg ggttagacaa
      361 tacgtcggca accaagttcg ccaatacgcg cgcgtcggct tcgttaaaac cgcgtgtggt
      421 catggcagcg gagccgatgc ggatgccgga ggtaacgaag ggtttttccg gatcgttcgg
      481 aatggcgttt ttgttgacgg tgatgtgcgc tttgcccaaa gcggcttcgg cggctttgcc
      541 ggtaattttc atcggttgca ggtcaacgag gaaaacgtgg ctttcggtgc ggccggaaac
      601 gatgcgcaaa ccgcgtttaa ccaactcttc cgccatggcg gctgcattga ttttcacttg
      661 ttttgcgtat tgtttgaact cgggttgcaa tgcttcttta aacgccacgg ctttggcggc
      721 gataacgtgc atcagcggac cgccttgcag gcttgggaag atggaagagt tcaacgcttt
      781 ttcgtgggta ttgtcgcggc acaaaattac gccgccgcga ggaccgcgca gggttttgtg
      841 ggtggtggtg gtcacgaagt cgcagaacgg caccgggttg ggatattcgc cgccggcaac
      901 cagaccggca tagtgcgcca tatcgacaaa gaggtatgcg ccgactttat cggcgatttc
      961 gcggaatttt gcccagtcga tttgtaacgc gtaggcagac gcacccgcca caatcatttt
     1021 gggtttgtgt tcgagcgcga ggcgttcgac ttcggcataa tcgagcactt cgttttcatc
     1081 caaaccataa gtaacggcgt tgtagagttt gcctgagata ttaacgctcg cgccgtgggt
     1141 caggtggccg ccgtgcgcta gagacatacc caaaatggtg tcgcctggtt ttaaaacgga
     1201 agcgtacacg gcttggttgg cttgcgagcc ggagtgcggt tggacgttgg cataggctgc
     1261 gccaaacagt tcttttacgc ggtcaatcgc caattgttcg acaatatcga cgtattcgca
     1321 gccgccgtag tagcgtttgc cggggtagcc ttcggcgtat ttgttggtca gctgggaacc
     1381 ttgcgcgtcc attacggcgc agctgacgta gttttcggaa gcaatcagct cgacgtggtc
     1441 ttgctggcgt tggtcttctt gggcaatggc tgctgccaaa tcggggtcgt attgtgcgag
     1501 ggtaacgctt tttgaaaaca tgttctcggc tcctttgtgt aatcagggta tcatgagtgt
     1561 tttttgtata aaaaaatatt tcaaaaccta aggcagatag cccataatgc gtaaattttc
     1621 tttggcatta tcaggtaatt tatttaacat gctggttttt agcgtctcat tacctttatt
     1681 tagtacaaga ctaatcaaaa gcaatacatt aaatggtaaa ttttcggcag tttgtgcaaa
     1741 atcaatcaaa taaccagcac caaccacatc tttatcagcc ataacccgct gatacaccac
     1801 ttgctaagac aactctcctg ccccaccatg tcttaaaatc agatgaatgg cttgtaatgc
     1861 tgtgtttggt gtctcacaaa ttgccaagat ctgctcagaa gcttgcttga tggcttgtat
     1921 ttttgtttga ttactcacaa tttcccccta ttttaataat taacttaaat gcggtcaaat
     1981 tcacaaaata caagctttac ctctaatcac ccatcactcg accttctcgg cgtggatcgg
     2041 caccaccaac cagcctgctt ggctcgataa taatggcttg aacacctgaa tttagctcac
     2101 gcacatcagt cttatagccc aaatcattta atgcttgttg ccactggacg gcggttgtac
     2161 ccgtttctag ttcatagcta ccaaagcgat ttaataaatt gggtgcactg atggcatttt
     2221 ggatatccat attccagtca ctatgtgcca caatcgtctt agcgacatag ccaatgatac
     2281 ggctaccacc tggggagccg attgccatat aaggcttgcc tgctttaaat acgatggttg
     2341 gtgccattga ggagcgtggt ctcttgccgg gctcgacacg attggcgacc tgtttgccct
     2401 gctttattgg ctcaaaacta aagtctgtca gctcattatt cagcaggtag ccatttgcca
     2461 tcaaagttga gccaaacgca ttttcaatgg aagtcgtcat tgatagcaca ttgcccgcct
     2521 tatccacaat tgatatatga ctggtagaag gtaactcaat cgcttgtgag gacacccact
     2581 catgaataaa atcgcctgca gatacgctag gcaatgcctt atccgactgt tcaagcagct
     2641 ggctgcgatg ttttaggtag tctttagaaa tcaactggcg aatgggtact ggtacaaaat
     2701 cagggtcgcc caaatataca tcacgatccg caaacgcaag cctagaagcg tcgcccaaga
     2761 gacgtaaacc ttcagcatca taccccacct gattgggtga aaattcattt aaaatcccca
     2821 aaatctgacc cacagcaatc ccacctgagc ttggtgcacc cataccgcat acttcataaa
     2881 tacgataagt cacacaaaca ggcgggcgtt ccaccacttg ataatcagat aaatcttgta
     2941 aggataattg accggggtta tccttagcat tttggacaac tgaaacgata ttttgggcat
     3001 atttaccagt atgcagagct tttgcacctt gagctgctaa cgcctgaaca ctgtcagcaa
     3061 attctaaatt tttcagcaag ctgcctgctt gtagcggcac accattcggc aaaaaataag
     3121 cggctgtttt tggatagcgt gccaaatgct gctgattttg ctcaaccgag atggcaagcc
     3181 ttggcgacac ctcaaagcct tgttttgcca agcggatcgg tgtatcaaat aattttcccc
     3241 aaggcaatac accgtatcgc tgatgtattg tctccatcag tttagggata gcaggcgtac
     3301 ccaccgagcg accaccgacc accgcttcca taaatttcaa tggttgacca tctttatcca
     3361 aaaataattc cggcgtcgca cgcatcggtg ccgtctcacg cccatcaaat gtggtcaatg
     3421 ttttggcggt attatcccaa tacaacacaa atgcaccacc gcccaagcct gacgactgtg
     3481 gctctaccaa gcttagtgtc gtctgcaccg ccaccatcgc atctgcagcg ctaccgcctt
     3541 gctttaagat atcatagcca gcttgtgttg ctaatggatt ggctgacgct accataaaat
     3601 cacttgcaat cacctgcttt tgttcggtca gtcccgttgc atgttcaggc gtgtgagcgt
     3661 ctgcacctgt gatgacagca gaatgagtat taaccttacc ttgattggca tggatgactt
     3721 gacatccaga gattgtcata gacattatca atgcagtcaa taaatatgtt ttagccacaa
     3781 gcactccttc gcctgagttt gattgatgat tcatacaagg catgctgatt attgtattta
     3841 atatggctaa ataattcaat ccaaactatc aatcttgacc atcaaaaaaa gaccgctaat
     3901 gtcatcagca gtcttttttg atatttattt taagatatta agtaatcaga cctttgggct
     3961 atgctcttca atgagtggtt ttagctcacc tgattggtac atttgtagga taatatcact
     4021 accaccgatt aactcaccat taacccaaag ctgtggaaag gttggccgac tggcgatgag
     4081 tggtagagta ctgcgaattt ctgggttttc taggatatta acaaaagcaa agggtctgcc
     4141 aattgggtca gcacctctac tgcacgcgct gaaaatccac attggggaaa ctggggcgtg
     4201 ccttt
//
LOCUS       DAWIVB010000167         4143 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWIVB010000167 DAWIVB010000000
VERSION     DAWIVB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.167"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..2397)
                     /locus_tag="WIX60_001782"
     CDS             complement(<1..2397)
                     /locus_tag="WIX60_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ5306009.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVTLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPAAAA
                     VVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAAGTS
                     AAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAGVLQ
                     GISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAAVGEMVGE
                     TLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVENNSLLARR
                     RVNIRWTSRQELEHEYAILEIQAITNQIRRLDPKFNGIAIMRNPREPWTRHDVQTYRQ
                     YYNQLRESRGFAVDPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGYFRVDSYN
                     PATEEIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNVPSTPTTIRGRKLEGKL
                     I"
     gene            complement(2542..2973)
                     /locus_tag="WIX60_001783"
     CDS             complement(2542..2973)
                     /locus_tag="WIX60_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306010.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(2989..>4143)
                     /locus_tag="WIX60_001784"
     CDS             complement(2989..>4143)
                     /locus_tag="WIX60_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ5306011.1"
                     /translation="AIGAAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSG
                     VVGGDVNAAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVA
                     DKRLAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYT
                     QADLALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNT
                     PITNVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQ
                     TDIEGITRIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGY
                     SKASKIAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          916 a          917 c          931 g         1379 t
ORIGIN      
        1 aataagtttt ccttcaagct ttcttcctct tatcgtagta ggtgtacttg gaacattgga
       61 aatgacagca ccagggctat attttctaac agcctccttg atataaccaa tccccgtact
      121 ttcttggatt tgagaaaatt gggtaaattt tcttgaaata atttcctctg tcgcaggatt
      181 atacgaatct accctaaaat accccgtcgc cgatttagga ttggctacat aaagctcatt
      241 ataagggtat tttgatgaca tgatacggtt aaattcattg ccgttgttta tcctgattct
      301 ataaattggg tcaacagcaa agcctctgga ttcccttaat tgattataat attgcctgta
      361 tgtttgtaca tcatgtcttg tccacggctc tctaggattc ctcataatag caatcccgtt
      421 aaatttcgga tccagccttc ggatttgatt ggtaatggcc tggatttcaa gaatggcata
      481 ttcatgttcc aattcttgtc gcgaagtcca acgtatattt accctcctgc gagctaaaag
      541 actattattc tccactgcca cagtagccgc attcgccgcc gtattcacat cccctttaac
      601 caatgccact gcgctgcctg cgataatctg cgagtaggct atgacttttt ggcgttcttg
      661 gggtgacagt ttgcctacat cgcgtccgtc caacagggtt tctcccacca tctcgccgac
      721 tgccgcgccg attgcgccgt cccgacattt gcctttattt gctaccgccg atgcacagcc
      781 tgctacggca tgggctatct tgtgggcaat gtagtcttcg ctgagattaa atttgatttt
      841 gctcgctact tctccgtgta cggtactgac tatcgcaccc agtgcggcat cgcccaagtt
      901 gtctttcagg ctgccgccgt tgatggcggt atggacactt gcggcagcgg tgctgttgat
      961 caggttagcg gtcagtttgc ctgctgcggg actgtgaaaa tgtttgctga cggcttcggc
     1021 tgcttgggtg ttcagcccgc ttatgccctg cagtacgcct gcggttacgg cggcggtggc
     1081 ggcctgtctg acggtgctgc ttttgcccag ttctttcagg gtatggttta tgtctccttt
     1141 gttgttgatg agggaaactg cggcttggct ggcgagactg gccagtgcgg ctttgcctgc
     1201 ggctgtggtg atagctgcag ctgatgtgcc tgctgctact ccggttgtgg ctgccgttcc
     1261 tgctgctact ccagttccgg ctgccgttcc tgctgcggct cctcccgctc ctgcgcctgc
     1321 cgcgcccgcc gttccggcgg ctgccggggc ggacagtgcg ccgtaggtca atacggttac
     1381 gacgataacg acgacagctg ctgctgcggg tgtcatgccc tcctgtttgt agtcccattt
     1441 atcgtaagca agctgcacct gattccagtt gatgtttttc gcaacttgaa gttgtttcaa
     1501 ataagcatac tcgggctgct tggtgagggt ttcgatttgg gttttcagat tgcctttcgg
     1561 aatatcgaca atgtaaccgc cgggtgcgga cagtacgggc gcaacgggac cggtgaaact
     1621 cggcaattgc aaggtttcga tgttactgcc ccgtcctgcc tgtttttgcc atagagtaga
     1681 tttgctgctg ctcacggttt ctgtgtggat gctgcttttg atcccttcga ggataatctt
     1741 ggcatcggcc cgtgcctgct cgcctacgcc tgcgcgtatg gttgcgccac ccagtgtggt
     1801 ttcaaactgt gtgccttgca gtttggtatc ccaacctgat tgcagattgg cagattctgc
     1861 aactaccctt gagggcagcg cggttttcat gacttgggtg gtggtgtcgt gtgctttgct
     1921 gtagctgatg ccgagaaatc tgcgcctttt ttggctgtca agtttgtcgt agttgagctc
     1981 ttctacggca tagagtgtca atttccgccc ggcttcgatg ttaatgctgc ctttgggggc
     2041 atcgaatgcg gtggcgtagg cgtcgatgct gccaccagat ttgatgtcga tgccttggga
     2101 tgcgctgagg gttactgcgt cgggcttggc gtttttgtgt tctttgactt cggtaatgtg
     2161 tttgcggttg taccatttgc cagttttgta gctgcggcgt tcaaaggtat agagttcgct
     2221 ctgtctcgca tagtaatatt ggtcaccgta agattgaatt ttgattttgc cgttttccga
     2281 actgatatcc gtggtgctga gcaggatgcg actgttctca ttggcatacg gtgcgctaat
     2341 gctcacaccg gttttacccg aaagttctgc agcaagcgta gggggtgtct tcagcacgca
     2401 cgcgtctggg cagtatatag ggaatctgaa tatttacttg cataacaaat gccgtctgaa
     2461 aaattgtgag cttttcagac ggcattgagc cgtaaatcat ggaacgcgtg cgtgctgaag
     2521 cacacacctt acgcatggat tttaggtttc atgcaggcta cagtttgttt gagaatatgt
     2581 tttttgtatt tctttacact ttttttgata ttcagggtga gctattaaga aatcagatat
     2641 tgcaatatta aagtaatgat atgctaaatt aataggtata attaattctt caggattgaa
     2701 atcatctcca actgaaaaat aaatagaagg agctttatca atatactcaa atccatttac
     2761 ctcttcataa tctccaatcc atctggtagc atctattttt tcatattgag cacctattac
     2821 tccaaaatgt ccggtagttg taatattttt aaaacactct aaaagcgtat ctttagtttc
     2881 aaattggtaa attgtttcaa aaaaaatcga aataacaaaa tggttgttat ttatagattt
     2941 tttatttaag tttagaaaaa tattattttt cataattttt cctttaaatt attctgggtg
     3001 aatgtttgta attcttcctg tatttacatc aaaatatgat ctaaatttga ttccgtcaaa
     3061 ggtttctgag aattgaatga cattttttct ttccgatatt gatttagttc tttcattttg
     3121 agcaatttta gaggcttttg aatatccttg tgaagcagca ttttgagcca tttgaagtat
     3181 tttatcatca gaaaattttt taggattata aacagttttt atacttgaaa tttccttaaa
     3241 tccaccatca ggtttacctg tcctgtctag tgtaggaatc tcatatttaa ttcgggtaat
     3301 gccttcaata tcagtttggg tttcagattt tacgcgtcct cctcgtgaat ttagttctgc
     3361 cataaaattg gtgcggttat gggctccttt aatgccctgt ttttgactaa aaccatcagc
     3421 atttgccaga tgtcttttta ggtttgtatc gaaactgatg ccttccgggt atttgacatt
     3481 agtaataggt gtattttgtt ttacaaaccc tcttggtatt ggaatgaatc taggattaac
     3541 tcctgaaata agtgtataac cttggtcgga catccattcg gataaaggct ttgtattgcc
     3601 cgattgaagc caagatttag cagcagtatt caaatgataa gactgtaaag ccaaatctgc
     3661 ttgggtgtaa gatttgctga ataatttata ccattcatca tctttaccaa ttagacctgc
     3721 ttcccaagat gaatgaaggc ttctactatc gatcaaatgt tgttttctga ttgttctaca
     3781 ttcagtacta cgggatatat ccgtacatat tgcaatcgaa gcagcaagtc ttttatcagc
     3841 aacattttga tactttttta cagtattttt tctgcacagt tgaggattat tctgtttggc
     3901 gcatgcagtc atttcgttat caaattctct accctctttg tcgctaagct gattattttt
     3961 caccgctacc tcagccgcat tcgccgccgc atttacatcg ccgccgacca caccgcttac
     4021 cgtaccggca accagtttgc tgtatgccaa aatctgttcg cgttctttag ctgtcaaagt
     4081 gtcaggattt ttgccgtttg tcaaagcctc cccgactatc tcgcccacag ccgcacctat
     4141 cgc
//
LOCUS       DAWIVB010000168         4128 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWIVB010000168 DAWIVB010000000
VERSION     DAWIVB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.168"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            43..675
                     /locus_tag="WIX60_001785"
     CDS             43..675
                     /locus_tag="WIX60_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ5306012.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WIX60_001786"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WIX60_001786"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ5306013.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIESLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2198..4066
                     /locus_tag="WIX60_001787"
     CDS             2198..4066
                     /locus_tag="WIX60_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ5306014.1"
                     /translation="MQTVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCYKK
                     TKSLRVLRHISIILFLILTASSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRGGALQ"
BASE COUNT          943 a         1117 c         1062 g         1006 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 atggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttccgaa tacggcctga
      841 tgaaggcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgccgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccaccacc aatcacgatg
     1021 tcaaagccat cgaatattgg ctgaaaaaac gttttgccga agtgccggaa gtcgccgccg
     1081 tgagtgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccatatggtc gcctatcctg atgtagattg ggaaacccac tgccgcaact
     1441 tcgtcgaaat cagcctcggt ctgaccttca acccctacac catccaaatc gaaccgcacg
     1501 actatatggc ggaattcttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcaggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaaccccat cgactttgaa aactccgagg
     1681 gcaacctcgg tatggcaaac gccgtattgg gctttttgtc cgaaaaactg ccgatttccc
     1741 gctggcagcg cgacctgacc gacagcaccg tattgcgcaa tatgggcgta ggcgtgggct
     1801 atgccgtatt gggtttcgcc gcccacctgc gcggtctgaa caagctcgaa cccaaccccg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggagctgct cgccgagccg attcaaaccg
     1921 taatgcgccg ttacggtgtc gccaatcctt acgaaaaact gaaagacctg acgcgcggca
     1981 aaggcggcat cacgcccgaa gtgctgaaag gctttatcga atcgttggaa attccggtag
     2041 aagccaaagc aaaattgctt gagctgactc ctgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacgggggc gatattgatg caaactgtcc gatacaggcc
     2221 tgaaattgac ggattacggg ctgtcgccgt cctctccgtc attattttcc acctgaataa
     2281 ccgttggctg cccggaggat tcctgggggt ggacattttc tttgtcattt cgggattcct
     2341 cattaccggc atcattcttt ctgaaataca gaacggttct ttttctttcc gggattttta
     2401 tacccgcagg attaagcgga tttatcctgc ttttattgcg gccgtgtcgc tggcttcggt
     2461 aattgcctct caaatcttcc tttacgaaga tttcaaccaa atgcggaaaa ccgtggagct
     2521 ttctgcggtt ttcttgtcca atatttatct ggggtttcag caggggtatt tcgatttgag
     2581 tgccgacgag aaccccgtac tgcatatctg gtctttggcg gtagaggaac agtattacct
     2641 cctgtatcct cttttgctga tattttgtta caaaaaaaca aaatcgctac gggtgctgcg
     2701 ccatatcagc atcatcctgt ttttgatttt gactgcctca tcgtttttgc caagcgggtt
     2761 ttataccgac atcctcaacc aacccaatac ttattacctt tcgacactga ggtttcccga
     2821 gctgttggca ggttcgctgc tggcggttta cgggcaaacg caaaacggca gacggcaaac
     2881 agcaaatgga aaacggcagt tgctttcatc actctgcttc ggcgcattgc ttgcctgcct
     2941 gttcgtgatt gacaaacaca atccgtttat cccgggaatg accctgctcc ttccctgcct
     3001 gctgacggca ctgcttatcc ggagtatgca atacgggaca cttccgaccc gcatcctgtc
     3061 ggcaagcccc atcgtatttg tcggcaaaat ctcttattcc ctatacctgt accattggat
     3121 ttttattgct ttcgcccatt acattacagg cgacaaacag ctcggactgc ctgccgtatc
     3181 ggcggttgcc gcgttgacgg ccggattttc cctgttgagt tattatttga ttgaacagcc
     3241 gcttagaaaa cggaagatga ccttcaaaaa ggcatttttc tgcctctatc tcgccccgtc
     3301 cctgatactt gtcggttaca acctgtacgc aagggggata ttgaaacagg aacacctccg
     3361 cccgttgccc ggcgcgcccc ttgctgcgga aaatcatttt ccggaaaccg tcctgaccct
     3421 cggcgactcg cacgccggac acctgcgggg gtttttggat tatgtcggca gccgggaagg
     3481 gtggaaagcc aaaatcctgt ccctcgattc ggagtgtttg gtttgggtag atgagaagct
     3541 ggcagacaac ccgttatgtc gaaaataccg ggatgaagtt gaaaaagccg aagccgtttt
     3601 cattgcccaa ttctatgatt tgaggatggg cggccagccc gtgccgagat ttgaagcgca
     3661 atccttccta atacccgggt tcccagcccg attcagggaa accgtcaaaa ggatagccgc
     3721 cgtcaaaccc gtctatgttt ttgcaaacaa cacatcaatc agccgttcgc ccctgaggga
     3781 ggaaaaattg aaaagatttg ccgcaaacca atatctccgc cccattcagg ctatgggcga
     3841 catcggcaag agcaatcagg cggtctttga tttggttaaa gatatcccca atgtgcattg
     3901 ggtggacgca cagaaatacc tgcctaaaaa cacggtcgaa atacacggac gctatcttta
     3961 cggcgaccaa gaccacctga cctatttcgg ttcttattat atggggcggg aatttcacaa
     4021 acacgaacgc ctgcttaaat cttcccgcgg cggcgcattg cagtagcctg ccttcttgtc
     4081 ggatattgcc tttggcagcc tatgccgctg tttgcccttc ggggcggc
//
LOCUS       DAWIVB010000169         4102 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWIVB010000169 DAWIVB010000000
VERSION     DAWIVB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.169"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..332)
                     /locus_tag="WIX60_001788"
     CDS             complement(<1..332)
                     /locus_tag="WIX60_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ5306015.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVDEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQG"
     gene            complement(313..594)
                     /locus_tag="WIX60_001789"
     CDS             complement(313..594)
                     /locus_tag="WIX60_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ5306016.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(603..1397)
                     /locus_tag="WIX60_001790"
     CDS             complement(603..1397)
                     /locus_tag="WIX60_001790"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ5306017.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1781..3115
                     /gene="gdhA"
                     /locus_tag="WIX60_001791"
     CDS             1781..3115
                     /gene="gdhA"
                     /locus_tag="WIX60_001791"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ5306018.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            complement(3250..4029)
                     /locus_tag="WIX60_001792"
     CDS             complement(3250..4029)
                     /locus_tag="WIX60_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ5306019.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWAV"
BASE COUNT         1062 a         1197 c          946 g          897 t
ORIGIN      
        1 ccttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggcaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgtttcaggg cttcgtccac gctgttttga
      121 ggaggcggcg tttccgcatc gggacgggcg gcaaaataat cggcatacag tttccacaac
      181 gcctgcactg tcggatcgaa cgcgccgtcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cgacggcgaa gtcagataac cgtccctgtt caggcggctg
      301 atcaggctgt cttcacggta aaggctgaac aattttttcc aaacgcgcca cttccgccaa
      361 aaccttgttg accaaatccg ccgcacgcct gtcgtccaca ccgaacagac ggagctccgc
      421 accggaaccc agtgcgacac ctttccagaa aaacacattt tcattgcgtt tttcatcccc
      481 gttgcgtttt tcatcatcgg cggcaaaagg attcggcagg aaagaaaccg ccgcgcccgc
      541 cgccgcaacg gcggcaaccg tcagaaaacg cctgcgcccg aaatgcctgc ccataccgcc
      601 tcctaaaccg acactgccgc cttgatatgc ggatgagggt cgtaaccttc caactcgaaa
      661 tcttcaaact tgaaggaaaa caaatctttg acttcaggat tgattttcat cactggcaag
      721 gcgcgcggtt cgcgttccaa ctgcaatgcg gcctgctcga aatggttgcg gtacaaatgc
      781 gcgtcgccaa acgtatggac aaactcgccc gcctccaatc cgcacacttg cgccatcatc
      841 atggtcaaca atgcgtagct ggcaatatta aacggcacac caaggaaaat atctgcacta
      901 cgctggtaaa gctggcagga cagtttgccg tcggcaacgt aaaactgaaa cagcgcgtgg
      961 cagggcggca aggccatttc atcgaccaaa gccggattcc acgccgatac aatcaggcgg
     1021 cgcgagtcgg gattcttctt gatttgttcc agcacattgg cgatttggtc gatatgcctg
     1081 ccgtcgggcg cgggccagtt acgccactgg tagccgtaaa ccgggcctaa gtcgccgttt
     1141 tcgtccgccc actcgtccca aatggaaaca ttgttgtcct ttaggtattt gatattggta
     1201 tcgcctttga gaaaccaaag cagctcgtgg ataatcgaac gcagatgcag ctttttggtc
     1261 gtcagcagcg gaaaaccttt gcccaagtca aaacgcatct gataaccgaa tacggagcgc
     1321 gtacccgtac cggtgcggtc tgatttgtcc gtaccgttgt cgaggacgtg gcgcatcaag
     1381 tccaaatagg ctttcatagc agtctttcat caaattaaac ggcgcatatt gtaacatttc
     1441 cggataatgc ccaaaacacg gatacaggca ggcaggattg ttggcaattt cagtcctttt
     1501 ccacagtaaa acccggtggg aaaacaaaat taccttgatt ggaatcaaaa aatctagttt
     1561 aattacttag aataaaattt caataatatt tttatttacg aaattaattt atgattattg
     1621 attaattatc ggcaacaaac atcaaacatc gaaaatatgg aaaaaataat gtcaacaatt
     1681 tttgccaaat cgggcttggc atcagaaaaa aataggttta tattcccacc tacaaatttg
     1741 ttttcccatt agtacactat caaccaaaag gagtatccga atgactgacc tgaacaccct
     1801 gtttgccaac ctcaaacaac gcaatcccaa tcaggagccg ttccatcagg cggttgaaga
     1861 agtcttcatg agtctcgatc cgtttttggc aaaaaatccg aaatacaccc agcaaagcct
     1921 gctggaacgc atcgtcgaac ccgaacgcgt cgtgatgttc cgcgtaacct ggcaggacga
     1981 taaagggcaa gtccaagtca accggggcta ccgcgtgcaa atgagttccg ccatcggtcc
     2041 ttacaaaggc ggcctgcgct tccatccgac cgtcgatttg ggcgtattga aattcctcgc
     2101 ttttgaacaa gtgttcaaaa acgccttgac caccctgcct atgggcggcg gcaaaggcgg
     2161 ttccgacttc gaccccaaag gcaaatccga tgccgaagta atgcgcttct gccaagcctt
     2221 tatgaccgaa ctctaccgcc acatcggcgc ggacaccgat gttccggccg gcgacatcgg
     2281 cgtaggcggg cgcgaaatcg gctacctgtt cggacaatac aaaaaaatcc gcaacgagtt
     2341 ttcttccgtc ctgaccggca aaggtttgga atggggcggc agcctcatcc gtcccgaagc
     2401 gaccggctac ggctgcgtct atttcgccca agcgatgctg caaacccgca acgatagttt
     2461 tgaaggcaaa cgcgtcctga tttccggctc cggcaatgtg gcgcaatacg ccgccgaaaa
     2521 agccatccaa ctgggtgcga aagtactgac cgtttccgac tccaacggct tcgtcctctt
     2581 ccccgacagc ggtatgaccg aagcgcaact cgccgccttg atcgaattga aagaagtccg
     2641 ccgcgaacgc gttgccacct acgccaaaga gcaaggtctg caatactttg aaaaacaaaa
     2701 accgtggggc gtcgccgccg aaatcgccct gccctgcgcg acccagaacg aattggacga
     2761 agaagccgcc aaaaccctgt tggcaaacgg ctgctacgtc gttgccgaag gtgcgaatat
     2821 gccgtcgact ttgggcgcgg tcgagcaatt tatcaaagcc ggcatcctct acgccccggg
     2881 aaaagcctcc aatgccggcg gcgtggcaac ttcaggtttg gaaatgagcc aaaacgccat
     2941 ccgcctgtct tggactcgtg aagaagtcga ccaacgcctg ttcggcatca tgcaaagcat
     3001 ccacgaatcc tgtctgaaat acggcaaagt cggcgacaca gtaaactacg tcaatggtgc
     3061 gaacattgcc ggtttcgtca aagttgccga tgcgatgctg gcgcaaggct tctaagcaaa
     3121 cgccgccgct ccgcaaacaa aatgccgtcc gaaccgcaaa tgctgttcag acggcatttc
     3181 cttatccgcc cgttcaaatc gggtgagact accgatacat ctgaatatgc tatgccgtct
     3241 gaacggcatt cacaccgccc aatcctgcac gcgcttcaaa tcattttgcg ccaaagtatc
     3301 tgcgtggcgg ttacggctct gatattccct gtctttcaag atgctgctcg ccacataatt
     3361 caaatgtgcc tttgccgcct ccgaagcctc gcccggccgg cggtttgata ttgcctcata
     3421 caatacacgg tgctgcgcca tcagcttcgg acgcggatct tcttcctgat tcagataaat
     3481 aaggctgctg cgcgtctgcc ggtacagcat tttcaacaaa ccgcccgaca aatggctgaa
     3541 caacaaattg tgcgccgcat cggcaatcgt ctgatgaaag ctgacatcag cttcgctctg
     3601 atgttccaaa ttgccgcttt cgcacgcctc ctcaaacttt tcaagccaaa acccaatccg
     3661 cttcaaatcg gcatccgtgc ggcgttctgc cgccaatgcc gccatacagc cctcgatgtg
     3721 gcaactgaaa tcaaaaacat cctgttccca attggaatgc ttgcccaaaa gctcctgcca
     3781 actttgcaaa aaatcctgct gcggcttgac cgaaacataa taaccgtctc cctgcctcgc
     3841 ttccaaaacc tgacgggcga ccaaaacatt caatgccgac ctgaccgacg ggcgcgaaac
     3901 gccgaactct tccgccaaaa cgcgttcggg cggaatcttg cccccttccg cgtaaacccc
     3961 ttccgcaatg cgctcctcca ataccgacaa tacctgatcg ctgattttct gaggccttac
     4021 cagtttcatc actcctcctt tataaagatt ccctgcagaa cccttccgaa atatagtgga
     4081 ttaacaaaaa tcaggacaag gt
//
LOCUS       DAWIVB010000170         3971 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWIVB010000170 DAWIVB010000000
VERSION     DAWIVB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.170"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            262..1779
                     /locus_tag="WIX60_001793"
     CDS             262..1779
                     /locus_tag="WIX60_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ5306020.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTPLADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1838..3619)
                     /locus_tag="WIX60_001794"
     CDS             complement(1838..3619)
                     /locus_tag="WIX60_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ5306021.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFANCADIDAESWLARHSDGLPAGIST
                     PQPSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RAENGRCTLLDRHLCRLKTSAQALNLPLPDGCENQIKQYIADLPDGAFRVKALLASDG
                     ISLSRAVLNRLTDKQRVIISPAVLPAQNYLRRFKTTCRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFIKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          836 a          890 c         1302 g          943 t
ORIGIN      
        1 agctcaaaga gaacgattct ctaaggtgct taagcacgag tgaatcggtt ccgtactatt
       61 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta taaatgtgaa
      121 atccgccctt tgaaaatcgg gcggcggttt ttgtttgcct gctttcaggc ggcaaagccg
      181 gttttcacgg gtttctgcct gtttttcgga tggtttgacg tgcttcggcg gcgtgtttgc
      241 cagaaaggta aatgacaggg tatgttgtat ttcagatacg gctttttggt tgtttggtgt
      301 gcggcaggtg tttctgccgc ctatggggcg gatgcgcccg cgattttgga tgacaaggca
      361 ttgttgcagg tgcagcggtc ggtgtcggat aagtgggcgg aatcagattg gaaagttgaa
      421 aatgatgccc cgcgcgtggt tgacggggat tttttgttgg cgcatccgaa aatgttggaa
      481 catagtttgc gcgacgcgct caacggcaat caggcggatt taatcgcttc gttggcggat
      541 ttgtatgcca agctgccgga ttatgacgcg gttttgtacg gcagggcgcg ggctttgctg
      601 gcgaaattgg cgggaaggcc ggcggaggcg gtggcgcggt atcgggaact gcacggggaa
      661 aatgcggcag acgagcggat tttgctggat ttggcggcgg cggagtttga cgatttccgg
      721 ctgaagtcgg cagaaaggca ttttgcggag gcggcaaaat tggatttgcc ggcaccggtt
      781 ttggaaaatg tggggcgttt tcggaaaaaa acggaggggc tgacgggctg gcgtttttcg
      841 ggcggcatca gtccggcggt caatagaaat gccaataatg ccgcgccgca atattgccgg
      901 caaaacggag gccggcagat atgcagtgtc agccgggcgg agcgggcggc agggttgaat
      961 tatgaaatcg aggcggaaaa gctgacgccg ttggcagata atcattattt gttgttccgt
     1021 tccaatatcg gcggcacgag ctattatttc agtaaaaaat cagcttatga tgacgggttc
     1081 ggcagggcgt atttgggttg gcagtataaa aatgcacggc agacggcggg gattttgccg
     1141 ttttatcagg tgcagttgtc gggcagcgac ggctttgatg cgaaaacaaa acgggtaaac
     1201 aaccgccgcc tgccgccgta tatgctggcg cacggagtcg gcgtgcagct gtcccatact
     1261 taccgcccaa acccgggatg gcaattttcg gtcgcgctgg aacattaccg ccaacgctac
     1321 cgcgaacagg atagggcgga atacaataac ggcaggcagg acgggtttta tgtttcgtcg
     1381 gcaaaacgtt tgggcgaatc ggcaactgtg ttcggcggct ggcagtttgt gcggtttgtg
     1441 ccgaaacgcg aaacggtggg cggcgcggtc aataatgccg cctaccggcg caacggtgtt
     1501 tatgccggtt gggcgcagga gtggcggcag ttgggcggtt tgaacagtcg ggtttccgcg
     1561 tcttatgccc gccgcaacta taagggcatt gcggctttct cgacagaggc gcaacgcaac
     1621 cgcgaatgga atgtctcgct ggctttgagc cacgacaagt tgtcgtacaa aggtatcgtg
     1681 ccggcgttga attatcgttt cggcaggacg gaaagtaatg tgccgtatgc gaaacgccgc
     1741 aacagcgagg tgtttgtgtc ggcggattgg cggttttgaa tggtgggata atgccgtccg
     1801 aactttggaa acaggttcgg acggcatttt tgcgcgttca ggcaagggcg gcggcaaata
     1861 cgccgcgcaa ggcgttggag aggcggattt cttcggcttc ttgcagtgtt ttttgtgtga
     1921 tgtgtgtttc gattacttga tttgtttgca aatatttttg cggttcgtcc aatacggctt
     1981 ggcgcattat gccgtttaaa atgtctaaat ctaaagaggg tgtgagccat tgtccgcgat
     2041 gtttgatgaa gacgttgctt ctgccgcctt cgagcaggat gccgtctgaa ttgaaaaaca
     2101 ggctgtcgaa cgcgccttgt gtttcggcgg tttgccacgc ttggtcgaag agggcgcggc
     2161 aggtggtttt gaagcggcgc aggtagtttt gtgcgggcag gacggcgggc gaaatgatga
     2221 cgcgttgttt gtcggtcaga cggtttaaaa cggcgcggga caggctgatg ccgtctgaag
     2281 cgagcagggc tttgacgcgg aacgcgccat cgggcaagtc ggcaatgtat tgtttgattt
     2341 gattttcgca gccgtcgggc aggggcaggt tgagggcttg ggcggaggtt ttcagacggc
     2401 ataggtggcg gtcgagcagg gtgcagcgtc cgttttccgc gcgcagggtt tcaaaaatgc
     2461 cgaagtcggg gcgcaattcg ttgaggaaac gggctttcca gccgcattcg cgatattcgg
     2521 cggcggggtc gctgtcgatg acgatgccgg aaccgacacc gtacacgcct tgataaatgc
     2581 cgtctgaaag cggcgtgagc gacaaggtgc ggataacgac gttgaacgtg ccttcaaacc
     2641 ccaagccgcc ggaacacggg ttcaaatagc cgatgctgcc cgtataaagt ccgcgcgctt
     2701 cggcttcgag cgattcgata atctgcatac tcattttttt gggcgcgccg gtgatgctgc
     2761 cgcaggggaa ggcggcgcgg aggatgtcgg cgaacgaggt gtgcggcaag gcttgggctt
     2821 ggatggtgct ggtcatctgc caaacgctgc cgaaacgcga tactttaaac ggttcgggta
     2881 cgcatactgt gccggtttgg gcgattttgc cgagatcgtt acgcagcaaa tcgacaatca
     2941 tcacgttttc ggcgcggttt ttcgggtctg cttgcaactc ggcggcgcgg cgttcgtctt
     3001 gtccgtcgcc caaaatcggc gcggtgcctt tcatcggttc ggtgctgatg gtgccgtccg
     3061 aaccgatttt gaggaagagt tcgggcgaga aacacagcgt ccacgcggat tgcccctccg
     3121 catcgggcag gtgggacaag acggcatagg ggacgggctg gcgcaggcgg cggtagaggc
     3181 tgacgggatt gccgtaggct tgcaggtgca ggcgggtggt gtagttgatt tgataggtgt
     3241 cgccgcgccg gatggcttcg tggatttggc ggatgcggtc gaggtaatcg gtttcggata
     3301 cggagggttg cggcgtggaa atgccggcgg ggaggccgtc tgagtgtcgg gcaagccagc
     3361 tttcggcatc gatgtcggcg cagttggcaa accagtgcag ggcaagattg ccgccgcgtt
     3421 cggactcaac ccccgtcagc ggcaaaccga atccgtagtc tgcaaacaac accgaatgca
     3481 gccctttttg ccagcccgat tgcagcgcgc cgtccaaagc atcgagttct tcgggacgga
     3541 aaaaacggct ttccacatga ttttgatagc gttttgcgcg gccgcttacg gcatcgtcaa
     3601 acagggcgaa ataaggcatg gcaatccggg gcaaatgttt tgattatacg ccccttatta
     3661 cacatatttt cagacaattc ggcaaatatc ggcaaaatgt aattttatgt agagaaagcg
     3721 ggggcgaagg tgtaaaattg tgaaaacaaa ttccgatttc caacctgaaa tacaataagg
     3781 agacctttat ggcagaccac cagctgcaac cgtttgaaaa cgtagaatta ggcgaaaagc
     3841 aagaccagct ccaagtatag tggattaaca aaaaccagta cagcggtgcc tcgccttagc
     3901 tcgaagagaa cgattctcta aggtgctgaa gcaccgagtg aatcggtttc gtactatctg
     3961 tactgtctgc g
//
LOCUS       DAWIVB010000171         3964 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWIVB010000171 DAWIVB010000000
VERSION     DAWIVB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.171"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WIX60_001795"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WIX60_001795"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ5306022.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFDLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WIX60_001796"
     CDS             1439..1924
                     /locus_tag="WIX60_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ5306023.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILAGVDGWLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1938..2045)
                     /locus_tag="WIX60_001797"
                     /pseudo
     CDS             complement(1938..2045)
                     /locus_tag="WIX60_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2089..3765)
                     /gene="ettA"
                     /locus_tag="WIX60_001798"
     CDS             complement(2089..3765)
                     /gene="ettA"
                     /locus_tag="WIX60_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ5306024.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNTKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMISGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          796 a          974 c         1108 g         1086 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggc
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgcga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgctgct gagcgcggcg ttgcctgaat tggatgaggt atggctggaa
      781 cggttttgcg gcagcttttt tgatttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacagaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccat ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacgg ccgttgtgtt ttttggcttt
     1501 cggtttcggt agcgggctgg ctccgttcgc gccgggcaca ttcggcactt tggcggcact
     1561 gcctttggca tttgtgttga tattggcggg tgtggacgga tggctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccac ggtgggattg tttgggacga gattgttgcc atgctgtttg tgttggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtt ctgttccgtc tgtttgacgc
     1801 gctcaaaccg ccccccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac cttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta tagtggatta aattcaaacc agtacggcgt tacctcgcct
     1981 tgccgtacta tttgtactgt ctgcggcttc gttgccttgt cctgatttaa atttaatcca
     2041 ctataaaaat gccgtctgaa agcctttcag acggcattgt ttcggaggtt aacgcgttac
     2101 cggtttgtat ttgatgcgtt tcggtttcgc gccttcttcg ccgagtcggc gtttcttatc
     2161 ggcttcgtat tcctgatagt taccgtcgaa gaacacccat ttggagtcgc cttcacacgc
     2221 caagatatgc gtggcgatgc ggtcgaggaa ccagcggtcg tgcgaaatca ccatcacgct
     2281 accggcaaat tccagcaatg catcttccag cgcgcgcagg gtttccacgt cgaggtcgtt
     2341 ggacggttcg tccagcagca acacattgcc gccgctcaac aaggttttcg ccaagtgcag
     2401 acggccgcgt tcgccgccag acaattgacc tgcgattttg ctttggtcgc tgcctttaaa
     2461 gttgaaacga cccaaatatt ggcgggcggg gatttcaaac tgcccgacct gtaaaatatc
     2521 gcggccttcg gcaatgttgt cgaatacggt tttgtcgttt tgcaaacctt cgcggctttg
     2581 gtcaatcagg ctcattttca cggtttgccc gattttcact tcgccggaat caggctgctc
     2641 tttgcccgaa atcattttga acagcgtcga tttacccgcg ccgttcgggc cgatgatgcc
     2701 gacaatcgcg cccgcaggca ctttgaagct caaatcgtca atcagcactt tatcgccgaa
     2761 cgatttggaa acatttacaa attcaatcac ttcgttaccc aaacgctcgg caacgggaat
     2821 aaagatttcc tgcgtttcat tgcgtttttg gtattcgtag ttgctcattt cctcaaaacg
     2881 cgccaaacgc gctttggact tggcttggcg gcctttggta ttttggcgca cccattccaa
     2941 ttcctgcttc atcgctttca cgcgcgcagc ttcggatttc gcctcgtttt ccaagcgttt
     3001 ttctttctgc tccagccaag acgagtaatt gcctttccac ggaataccgt gcccgcggtc
     3061 aagttccaaa atccattcgg cggcgttgtc gaggaagtag cggtcgtgtg ttaccgcaac
     3121 gactgtaccg gggaagcgca cgaggaattg ctccagccat tccaccgatt ccgcatccaa
     3181 gtggttggtt ggctcgtcca gcagcagcat atcgggcttg ctcaacaaga gtttgcacaa
     3241 agcgacgcgg cgtttttcac cgccggacag attgccgatt ttggcatccc attccggcag
     3301 gcgcagcgcg tcggcagcga tttccaattc gtgttccgca ccgccgcccg tggacgaacc
     3361 cgccgcaata atcgcttcca aacgcccctg ctcttctgcc aacgcgtcaa aatccgcatc
     3421 gggattggcg tactcggcat acacttcttc caaacgtttc tgcgcggcag ccacttcgcc
     3481 caaaccgctt tccacttcct cgcgcacggt tttttccgga tcaagctcag gctcttgcgg
     3541 caggtagccg attttgatgc cgcccatcgg cacggcttcg ccctcaaatt ccttatccac
     3601 gcccgccata atccgcagca cggtggactt gcccgcgccg ttcaaaccga gcaggccgat
     3661 tttcgcgccg gggaagaaag aaagggaaat atctttaatg atggttttct gcggcggcac
     3721 aaccttgctc acgcgcagca tagaatagac gtattgttgg gacatggttt tctcgttttc
     3781 atcaaacaaa tttcagacgg ccattttaac cgataatttg atttaagcca gtttatccgc
     3841 gaaccggtat tgccaaaatc gggcaggatt cataaaatcc gcttatccct ttgaaattat
     3901 atagacaaaa aaataataat gataggggat cgccgccccg gcaaccattt cggattttcc
     3961 aaag
//
LOCUS       DAWIVB010000172         3938 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWIVB010000172 DAWIVB010000000
VERSION     DAWIVB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.172"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(473..1513)
                     /gene="queA"
                     /locus_tag="WIX60_001799"
     CDS             complement(473..1513)
                     /gene="queA"
                     /locus_tag="WIX60_001799"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ5306025.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRHAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            1630..1887
                     /locus_tag="WIX60_001800"
     CDS             1630..1887
                     /locus_tag="WIX60_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ5306026.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            1869..2324
                     /locus_tag="WIX60_001801"
     CDS             1869..2324
                     /locus_tag="WIX60_001801"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ5306027.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2436..3797
                     /gene="accC"
                     /locus_tag="WIX60_001802"
     CDS             2436..3797
                     /gene="accC"
                     /locus_tag="WIX60_001802"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ5306028.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGLIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
BASE COUNT          949 a         1194 c          959 g          836 t
ORIGIN      
        1 aatttatctt ataaacaaac aaaggcagcc tgaaagattg aatggtctgc acccctaagg
       61 ttggactaac caaccgacta aggtgcagat tattttttgt tgctttttca gcttttcgtt
      121 gggttagata ttcttgccca ctgttttcag gcagccttga atacaaaaaa atggcgtatg
      181 taatatgttt atacgaccaa aacggaatga attttaacgt attgcgcgtc atcaacaatg
      241 actgagtttc tcgcctctcg cgcctgaatc tatagtggat taacaaaaac cagtacagcg
      301 ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgttgccttg tcctgattta
      361 aatttaatcc actatatgtc atgcagttcc tttcattcaa atcaacaaaa gaatgccgtc
      421 cgaacgtccg ttcagacggc acttgtcttc ccacaataga cttgaggctg ttctaacgta
      481 ccaccccttc gttccgcccc aaaaccatcg catcgccgta gctgaagaaa cggtattcgc
      541 gttcgaccgc atgacgatac gcggcgcgga tatgacccat acccgaaaac gcgctgacca
      601 acatcagcag cgtcgatttc ggcaaatgaa aattggtaac cagtctgtcg acaacattaa
      661 aacggtagcc cggcgtgatg aaaatatcgg tgtcgccctg ccccgctttc agacgacccg
      721 tcgcacgcgc ggcagattcg agggcgcgca tggaagtcgt gccgaccgcc cagactttgt
      781 tcccccgggc ttttgccgcc tcaacggcgg cggcggtttc agacggcact tcaaaccatt
      841 cgctgtgcat tttgtgctct tcgattttgt ccacacgcac gggttggaac gttccggcac
      901 cgacgtgcag ggttacttct gcggttaccg cgcctttgtc tttcagacgg tgcaaaagtt
      961 cttccgtaaa atgcaggccc gccgtcggcg cggcgaccgc gccctgatat ttggcataaa
     1021 cggtttgata acggctgtcg tcatccgcat cggcggcgcg ttcgatataa ggcggcaggg
     1081 gcaggtgtcc gttctgttcc aaaagttcgt aaacggtctc tccgccttca aaacgcaggc
     1141 agaacagttc gccctcacgc ccgaccgtca cggcgcggat gccgccttca aacaccagcc
     1201 ccataccggg cttgggcgat ttggacgaac ggatgtgcgc cagtgcggta tggttgtcca
     1261 acacgcgctc aatcagggct tcgatcctgc cgccgctgtc tttctgccca aacagccgcg
     1321 ccttcatgac tttggtgttg ttgaacacca aaacgtcgcc cgcctcgaca taatccggca
     1381 aatcgccgaa cacccggtct tgcagcggca tatcgggcaa cgcaaccaaa aggcggctgc
     1441 tgccgcgcac ttcgggcgga tgctgggcaa tcagcttttc gggcagggta aaatcaaaat
     1501 ctgaaatatc catttttaca ctctcgttcg ggcaagccgc cattatacgc actttagccc
     1561 tttttcagac ggcatctttg tccgaaaaac caacagatta gaataaacac tcttaacctg
     1621 gaacatcttg tgcgcaaaat caaacttcct gcacatttcc cccaaaaacc gccgtttttt
     1681 gatattttac tggacattta ccgacaactt cgggaaaata aacacattct cacggtcgtt
     1741 ttccaccaca ggaaaaccgt atccgaacac cattccgccc ggtttgcgcc gttgccgcaa
     1801 gccggctgtt ttctgaaaaa ccaacgcaac aacccgccgg aacaccggca gcctttaaag
     1861 gaacagaaat ggatttgcgc aaattaaaaa aactgattga tttggttgaa gaatcgggta
     1921 tcgccgaaat cgaagtaacc gaaggcgagg aaaaagtccg catcacccga accatcgccg
     1981 ccgcacccgt ttacgccgcg cccgtacctg ccgccgcgcc ggccgtaacg cctgccgccg
     2041 cacccgttgc ggcatccgcg cccgccgccg cacctgccgc ccgcgatttg tccgacgcgc
     2101 aaaaatcgcc tatggtcggc acgttctacc gcgcacccgg cccgaatgcc gcgccttttg
     2161 tcgaagtcgg ccaacaagtt aaagccggcg acacgctgtg catcatcgaa gcgatgaagc
     2221 tgatgaacga aatcgaagcc gaaaaatccg gcacggtcaa agaaattttg gtcgaaaacg
     2281 gtacgcccgt cgaattcggc gaaccgctct tcattatcgg ataatcctgt tttcagacgg
     2341 cataaacttc cgatgccgtc tgaaatgctt tcccccttca gcgttcccgc accctttttt
     2401 acggacgggt tgccggaacc gcaggaaagg tcatcatgct gaaaaaagtt ttaatcgcca
     2461 accgaggcga aatcgcatta cgcgtactcc gtgcctgccg cgaaatgggc attgccaccg
     2521 tcgccgtgca ttccgaggcc gacaaagaca gcctgcacgt caaactcgcc gacgaatccg
     2581 tgtgcatcgg ccctgccgct tccgcgcaaa gctaccttaa cgtccccgcc attatcgccg
     2641 ccgccgaagt aagctgcgcg gacgctgtcc atccgggtta cggtttcctt gccgaaaacg
     2701 ccgatttcgc cgaacaggtc gagcagtccg gctttacctt tatcggcccg aaacccgaca
     2761 ccatccgcct gatgggcgac aaagtctccg ccaaacacgc gatgatagcg gcaggcgtac
     2821 cctgcgtccc cggttctgac ggcgcattgc ccgacgacgg cgaagaaatc ctcaaaatcg
     2881 ccgataaagt cggttatccc gtcattatca aagcctctgg cggcggcggc ggccgcggta
     2941 tgcgcgtggt cgagaaaaaa gaagacctcc tccaatctgt cgaaatgacc aaagccgaag
     3001 caggcgcggc attcggcaac ccgatggttt acatggaacg ctatttgcaa cgtccgcgcc
     3061 acgtcgaaat ccaagtgatt gccgacgaac acggcaacgc catctacctt gccgagcgcg
     3121 actgttcgct gcaacgccgc caccaaaaag tcatcgagga agcaccggct ccgttcatca
     3181 ctgaaaaaga acgcgccaaa atcggcaacg cctgtgccga tgcctgcaaa cgcatcggct
     3241 accggggcgc gggtacgttt gagtttttat acgaagacgg cgaatttttc tttatcgaga
     3301 tgaacacgcg cgttcaggtc gagcatccgg ttaccgagct catcaccggc gtggacatcg
     3361 tgcaggagca actccgcatc gccgccggcc tgcctttgca atacaaacaa aaggatattc
     3421 aagtcgaagg ccacgcgttt gagtgccgta tcaacgccga agacccgtac aacttcattc
     3481 caagcccggg cctgattgaa agctgccacc tgcccggcgg cttcggtatc cgcgtggaca
     3541 gccacattta ccaaggctac cgcatcccac cgtactacga cagcctgatc ggcaaaatct
     3601 gcgtacacgg caaaacgcgt gaacaggcaa tggcgaaaat gcgcgtcgca ctcgccgagc
     3661 tggcggtaac cggcatcaaa accaatacgc cgcttcaccg cgacctgttc gccgatgcgg
     3721 gtttccaaaa aggcggcgtc agcatccact atttggaaca ctggctggaa gatcgcaaag
     3781 ccaaacagga caagtaaacc gccgccgata tgccgtctga agccgcccgt ccgcgttcag
     3841 acggcatttc ccttgccccg cgccgtctga aaccgatttc gatatagtgg attaacttta
     3901 aaccagtacg gcgttgcctc gccttagctc aaagagaa
//
LOCUS       DAWIVB010000173         3829 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWIVB010000173 DAWIVB010000000
VERSION     DAWIVB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.173"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            362..1483
                     /gene="dnaJ"
                     /locus_tag="WIX60_001803"
     CDS             362..1483
                     /gene="dnaJ"
                     /locus_tag="WIX60_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ5306029.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1679..3697
                     /locus_tag="WIX60_001804"
     CDS             1679..3697
                     /locus_tag="WIX60_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ5306030.1"
                     /translation="MNKSLSSSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIVIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
     gene            complement(3741..>3829)
                     /locus_tag="WIX60_001805"
     CDS             complement(3741..>3829)
                     /locus_tag="WIX60_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ5306031.1"
                     /translation="QTAPLLSPKDLAGKTWAQAEKLRLPLYR"
BASE COUNT          810 a          862 c         1149 g         1008 t
ORIGIN      
        1 gcggtgctgg cgaagctgaa ggatcaggct tccgtcggcg cgcccgcacc aaaaggccca
       61 gccaaaccag tgctgcccgc ggataaaaaa taaaataggg ggaagtctgc agccgcatca
      121 aatgccgtct gaacatgcgt tcgggcggcg tttttataac aaaaacactt catggcggtt
      181 ggttttatgc ctatctaagt ttttgtgtcg tgcatacctg aagatttcag acggcatcgg
      241 tttatgctgt ctgaaaagtg tattccgttt cagtttgtaa gctatggcag tctgtttgtc
      301 ttgtgttttg cgcaattgcc cttattttga gccgtgattt tattttgaat tagatgaaaa
      361 aatgagtaat caagattttt atgcgacgct gggtgtggca agaacagcta ccgatgatga
      421 gattaaaaaa gcctaccgga aattggcgat gaaataccat cccgaccgca atcctgacaa
      481 taaagaggcg gaagagaagt ttaaagaagt acaaaaggcg tatgaaactt tgtccgacaa
      541 ggaaaagcgc gctatgtacg accagtatgg tcatgcggcg tttgaaggcg gcggacaggg
      601 gggcttcgga gggtttggcg gatttggcgg tgcgcagggt tttgactttg gggatatttt
      661 cagccaaatg tttggaggcg gttcggggcg cgcccagcct gattatcagg gtgaggacgt
      721 tcaagtcggt atcgaaatca cgcttgaaga agccgcaaaa ggtgtgaaga aacgcatcaa
      781 tattccgact tatgaagcgt gtgatgtctg taacggcagt ggcgcgaaac cggggacatc
      841 cccggaaacc tgcccgactt gcaaaggttc gggtacggtg cacatccagc aggcgatttt
      901 ccgtatgcag cagacttgtc cgacctgcca cggtgcgggc aaacacatta aagaaccttg
      961 cgtcaaatgc cgtggcgcgg ggcggaataa ggcggtcaag acggtggaag tcaatattcc
     1021 cgccggtatc gatgacgggc agcgtatccg tttgagcggc gaaggcgggc cgggtatgca
     1081 cggtgcgcct gccggcgact tgtatgtaac cgtccgcatt cgggcgcata agattttcca
     1141 acgcgacggt ctggacttgc attgcgaact gccgatcagt tttgccacgg ctgctttggg
     1201 cggggagttg gaagtgccga ccttggacgg aaaggtcaag ctcaccgtcc ccaaagaaac
     1261 ccaaaccggc aggaggatgc gcgtgaaggg taagggtgtc aaatctttac gcagcagcgc
     1321 gaccggcgat ttgtactgcc atattgttgt cgaaacgcct gtcaatttga ccgaccgtca
     1381 aaaagagctt ttggaagaat ttgagcggat ttctaccggc ttggaaaacc aaacaccgcg
     1441 caagaaatcg tttttagaca agctgcgcga tttgtttgat tgattttaag gttcggaaac
     1501 aagcagccgt atcggggaat ctccttgata cggctgtttt tatttgttta aaaatagttt
     1561 ttattttcaa tggggtatga ggcagggtgg gataactgtt tttaactgtt ctttttaaaa
     1621 cttgacatca tggcgtgatg ccaacaatat gtgaacgtct gttgtcaaag gaagaataat
     1681 gaataaatct ttatccagtt cggtagaaga ataccgcgag ctgacgctcc gaggcatgat
     1741 actcggtgca ttgatcactg taatttttac tgcgtccaat gtttacctcg gtttgaaagt
     1801 cgggctgacc tttgcctcgt cgattccggc ggcggtgatt tcgatggcgg ttttaaagtt
     1861 tttcaaaggc agcaatattt tggaaaacaa catggtgcag acccaagcct cggctgcggg
     1921 tacgctttcg accatcatct tcgtcctgcc cggtttgctg atggcgggct actggagcgg
     1981 tttcccgttc tggcagacga cgcttttatg tattgccggc gggattttgg gggtgatttt
     2041 caccattcct ctgcgttacg caatggtggt gaaaagcgat ttgccttatc cggaaggtgt
     2101 ggcggctgct gaaattttga aagtgggcgg tcatgaagaa ggggataacc gtcagggcgg
     2161 cagcggcatc aaagagctgg cggccggcgg tgcgttggcg ggattgatga gcttttgcgc
     2221 cggaggtctg cgcgtgattg ccgacagcgc gagttattgg tttaaaagcg gtacggcgat
     2281 tttccagctg ccgatgggct tttcactggc attgttgggc gcgggctatt tggtcggact
     2341 gacgggcggt atcgccatcc tgttgggcat ttcgattgct tggggcattg ccgtgccgta
     2401 tttctcctca cacattccgc aaccttccga tatggaaatg gcggcgtttg cgatgaagct
     2461 gtggaaggag aaagtgcgtt ttatcggtgc ggggactatt ggcattgcgg cggtttggac
     2521 gctgttgatg ctgctcaagc cgatggtgga aggcatgaag atgtcgttca agagttttgg
     2581 cggcggtgcg cccgctgcgg aacgcgccga acaggatttg tcgcctaagg ctatgatttt
     2641 ttgggtgctg gcgatgatgt ttgttttagg cgtgtcgttt taccacttta tcggcgattc
     2701 gcacattacg ggcggcatgg cttggctttt ggtggtcgtt tgcacgcttt tggcttccgt
     2761 catcggcttt ttggtcgccg ccgcctgcgg ttatatggca ggtttggtcg gctcgtcttc
     2821 cagcccgatt tccggcgtgg gcatcgtgtc cgtcgtcgtt atttcactgg ttttgctgct
     2881 ggtaggcgaa tccggaggtt tgttggcgga tgaggctaac cgcaaatttt tgctggcact
     2941 gactttgttt tgcggctcgg cagtaatctg cgtggcttcg atttccaatg acaacctgca
     3001 agacttgaaa accggctacc tgctcaaagc cacgccttgg cggcagcaag tcgccctgat
     3061 tatcggctgt atcgttggtg cgctggttat ttcgcccgtg ttggaactgc tttacgaagc
     3121 ctacggcttt accggcgcaa tgccgcgcga aggcatggac gcggcgcagg ctttggcagc
     3181 ccctcaagcg actttgatga cgaccatcgc gtcgggcatt ttcgcccaca accttgaatg
     3241 ggtctatatc tttaccggta tcgtgattgg agcagtatta atcgtcgtcg atttggtgtt
     3301 gaaaaaatca tcaggcggca aacttgccct gcccgtcctt gcggtcggta tgggtattta
     3361 tctgccgccg tccgtcaata tgcccatcgt ggcaggcgcg gtgttggcgg cggtgttgaa
     3421 acacatcatc ggtaaaaaag cggaaaaccg cgaaggccgt ctgaaaaacg ccgagcgcat
     3481 cggaaccttg ttctccgccg gcctgattgt cggtgaaagc ctgatcggtg tgattatggc
     3541 gtttattatt gccttctccg tgaccaacgg cggctcggat gcgccgctcg cgttgaatct
     3601 gcaaaactgg gatgccgccg cttcttggct gggtttggcg ttcttcgtta ccgggatgtt
     3661 tttctttgca cagcgcgtac tgaaggcggg caagtaggct gtcggaaaaa atgccgtctg
     3721 aaacgttcag acggcatttt ttatcggtaa agcggaaggc ggagcttttc ggcttgcgcc
     3781 cacgttttgc cggcaaggtc tttgggcgac agcagcggcg cggtttgaa
//
LOCUS       DAWIVB010000174         3828 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWIVB010000174 DAWIVB010000000
VERSION     DAWIVB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.174"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            26..469
                     /locus_tag="WIX60_001806"
     CDS             26..469
                     /locus_tag="WIX60_001806"
                     /inference="COORDINATES: protein motif:HMM:NF016736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ5306032.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLSRRNPTTASGTLTVSGIAQSMLSDD
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARRN"
     gene            469..1080
                     /locus_tag="WIX60_001807"
     CDS             469..1080
                     /locus_tag="WIX60_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ5306033.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVSSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPSDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            1077..1637
                     /locus_tag="WIX60_001808"
     CDS             1077..1637
                     /locus_tag="WIX60_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ5306034.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQHRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            1648..3621
                     /locus_tag="WIX60_001809"
     CDS             1648..3621
                     /locus_tag="WIX60_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ5306035.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVITGDQTVNGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIQGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     ALGYEDRNERQRSALVSTWTDENLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSERVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
BASE COUNT          928 a         1178 c         1074 g          648 t
ORIGIN      
        1 cccgtgattt aaagaggttt taaacgtgtt tgaaacgccg acatttgagc aaatccgcga
       61 gcgtatcctg cgcgatacca aaagcctgtg gccggatgcc gatatcagcc ccgacagcga
      121 ccattatgtg cacgccagcc gtttggccag ctgcgccgaa gggcaatatg cgcatcaaag
      181 ctggattgtg cggcagattt tccctgatac cgccgaccgc gagtatttgg agcggcatgc
      241 ctccatgcgc ggcttgagcc gccgcaatcc taccacggcc agcggcacgc tgaccgtaag
      301 cggtattgcg caatccatgc tttcagacga cctgcaagtg cgtatcggcc agcgttttta
      361 ccgcactacc gcccgcgccg ttatcggcag cggcggcacg gcggaaatac cggcaatcgc
      421 cgacgagccg ggcgcggccg ccaatgtggc gacggcgagg cgcaactgat ggccgccccc
      481 gccggtgtgg ccaccgaatg ccgccttacc gtacaaggcg gcaccgaccg agaaagcgat
      541 gcctcactgc tggcgcgtct gttggaaatc atccgccgac cgcccgcagg cggcaaccgt
      601 tacgactata aaaactgggc gttgagtgtt gacggcgtaa ccagcgcata tgtttatccg
      661 ctgcgccgcg gcttgggtac ggtggatatt gccattacct ccgccgacgg tgtgtcgtcg
      721 gaagaaactg tgcgccgcgt acaggcttat atcgacgaga tgcgcccggt aacggcaaaa
      781 aatgcgctgg tactcaagcc aaccgtaacg gcggtgcctg ttaccgtgca agtcaagctc
      841 gacggtatcg acttggacga ggccaagcgc cgcatacgga cggccctaaa agaatatttc
      901 gacaccctga tccccagcga cggcctgact gtgtcgcaaa tcgaggctgc tatcagcaat
      961 gtggatggtg tgatcgaccg ccgtctgact gcgccgacgg ccaaccgtgc cgccgatacg
     1021 gttaaccgca tcgagtggtt taaagcgggc gcgattaatg taacggagat gccgtcatga
     1081 gctatcaaga catcttgcgg ggcctgttgc cccccgtgtc gtatgcccgc aatgccccgc
     1141 gtgtgcgggc gcaggcagaa atagacggcg cagcgctgga tgcggtggcg gaatcggctc
     1201 aaagcgttgc cgatgccgtc gacccgcgca gcgccggcca aatgctggcc gattgggagc
     1261 gcgtattagg tttggacggt acgggcaaaa accgccagca ccgtgtgttg gccgtcatgg
     1321 ccaagctaaa cgaaacaggc ggcttgagta ttccttattt tgtgcgtttg gccgaggcgg
     1381 cgggctatca aatccaaatc gacgaaccgc agccgttccg cgccggtgta aaccgcgccg
     1441 gcgaccgtct tgcgccgcag gaaatcatgt gggtgtggca cgttaacgtg cgcggcggca
     1501 acaaccgcat tacccgattc cgcgccggta tctcggcggc gggcgacagg ctgaccgatt
     1561 acagcgatgc cgtgatcgag agcctgttca accgcctcaa gcccgcccac accgctatcc
     1621 gatttaccta ccgctaaagg acgatttatg caccccatcg aaacccccga taagaccttc
     1681 cacgacggcg acggcgtgtc cgaattgggc accatcctgc ccgcgtggtg gctcaaccaa
     1741 gtgcaatccg agctgctggc cgtgctgact gcggccggta tccagccgga taagtcgcag
     1801 cctaatcaat tactggcagc actaaatagg ctggctgtag ttatcaccgg cgaccaaacc
     1861 gtcaacggcc aaaaaacctt taccgcccaa acccaattcc aaagcggcat ccatttatcc
     1921 gccaaccaga cgaactggaa cggcggccac aaagcctaca tcggcgcgga tgccgacaac
     1981 gcccacatcg tcttcggcga cgacaccctc cgcctgcacg gcgcaaacaa ccgcatttcc
     2041 tacaacaacc acgacatctt ccacaaagcc aacaaaccgc gttttgccga agacatccaa
     2101 ggcaaaccga acacactgtc cggatacggc atcggcaatt tcaaagtcga aacattccgg
     2161 ggcgatttga acaccctcaa aacagacggc atctattccc tgccgacggc ggtcggcagc
     2221 tccaacctgc ccgttgaaaa caccgcctgc catatccaag tcatcgccgg cacgaaacac
     2281 ggctggtgca ggcagttggg ttatcccgcc tacacgtccg acgtgtacga acgccaccaa
     2341 acgagcagcg caaacgacaa ctggtccgca tggaaaaaac tcaattcgga cggcatcccc
     2401 gtcggcgcga tcgtatcctt tcccaaagcc gtacaaaacc ccgcaggcta tctcaaagcc
     2461 aacggcacga cctttgcaca aaacaccttc cccgaccttt accgcgcctt gggcaacagc
     2521 aaccgcctgc ccgatttaag ccgtaccgac atcggcatca ccgcgtggtt tccgtccgac
     2581 caaatcccga ccggctggct ggcgtttgac gacatccgca cgcgcgtaac cgaaaccgct
     2641 tatcccgagc tgtaccgtct gctgaccgga aaatacggca gcatccaaaa cgtcccgcag
     2701 gcggaagacc gctttatccg caacgcgggc aacagcttgg cagtcggaac gaagcaggaa
     2761 gacgaaatca aacggcacgt ccacaaagta ttttcacact ggacaaacca cacagacgcg
     2821 gcagccctcg gttacgaaga ccgcaacgaa aggcagagaa gcgcgctcgt atcgacttgg
     2881 acggacgaaa atttaaacga caacggcttt ttaaccccgc gctcggacag caaaatggcg
     2941 acaggcggcg acgaaaaccg ccccaaagcc ctggttttaa aactgtgcat caaagccgcc
     3001 gacaccttgg gcgaagccgt gttttggata aagtcccacg gcgaaaccat caacgcaggc
     3061 gcgctggacg cgggcacgct ggcgcaaggt ttgcaagaca aagccgaccg agaccacacc
     3121 cacaccgccg cccaaatcca agggctggac gaaaaaatca gcaccgccgt tgccgcgcaa
     3181 ttcacacgcc aaaccatcgg cggcgtggat attgtcagat tccccgacgg cacaatgata
     3241 cagaccggca gctacaggtt cacacgaagc ggcggcccca tcgaaaacga agtcgtcttc
     3301 cccgtcgcct ttgccgacgg caacgtcaaa tgcttcgtat ccgaacgcca ttcggaacgc
     3361 gttaccggcg atcgaaggca acacaactgg ctgtttatcc gcgcaaaaaa ccacgccgcc
     3421 gccattatca ccaactggta cgaaggcagt tgcgactgga tggccatcgg caaagccgcc
     3481 tcgggaaacg ccgccagctc cccgataggc cccgaaatac ctgaaaccaa cgaagaaccg
     3541 caaagagaga gtggaagaac atcaaccgga ccccgaaacc gccgccgccg agacggcttg
     3601 ctcgaggcac tgcaagacta gcgggctgta gagatggctg tagagacggg ctgtagagat
     3661 ggctgtagag acgggttgta gagacgggtt gtagagatgg gttgtagaga tggctgtaga
     3721 gatggctgta gagatggctg tagagatggg ctgtagagat gggttgtaga gatgggttgt
     3781 agagatgggc tgtagagatg ggctgtagag atgggctgta gagatggg
//
LOCUS       DAWIVB010000175         3787 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWIVB010000175 DAWIVB010000000
VERSION     DAWIVB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.175"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            142..1285
                     /locus_tag="WIX60_001810"
                     /pseudo
     CDS             142..1285
                     /locus_tag="WIX60_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(1395..1604)
                     /locus_tag="WIX60_001811"
     CDS             complement(1395..1604)
                     /locus_tag="WIX60_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306036.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1601..1897)
                     /locus_tag="WIX60_001812"
     CDS             complement(1601..1897)
                     /locus_tag="WIX60_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ5306037.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRRARL
                     AQKPKTRWKILYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1876..2481)
                     /locus_tag="WIX60_001813"
     CDS             complement(1876..2481)
                     /locus_tag="WIX60_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306038.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSDYHEWDGKKWKISKAAAAARFAKQKTALAFRLAEKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            2561..2848
                     /locus_tag="WIX60_001814"
     CDS             2561..2848
                     /locus_tag="WIX60_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ5306039.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2820..3122
                     /locus_tag="WIX60_001815"
     CDS             2820..3122
                     /locus_tag="WIX60_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ5306040.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYIAQLK"
BASE COUNT          782 a         1000 c         1045 g          960 t
ORIGIN      
        1 gcaacgctgt actggtttaa atttaatcca ctataaatta acacgcaatg taccctgacc
       61 ggcaaaactt ccccaaactt catcaaaaaa tcaactgtac ccgtcttttt cagacggcat
      121 tgataccgta agaaatttcc catgcagaaa tggcaaaccg agctttattc cacaccgctt
      181 tggctttgtc tgacaccgaa aagcgcggtc aaagtcttgc tgctgattac ggcgatgatt
      241 acgctggtgt taaccgaaat gcggctgaat gtgttgagta cctttatgtc caaagggctt
      301 tacgactcga tgcaggattt gaaatgcttc ggcattttgg atgtttgctg caatgaacgc
      361 gggcgtggtg ctgatacggg cgtttaacaa cgtcgtcaac gacttcctcg atcaaggctt
      421 ggcgattaaa tggtcggagc ggctcaacga agtgctgaca gcacgctggc ttgccgacaa
      481 aaactactac cgcctgcaaa tgcgccgcca cgcgccggac aacatcgacc agcgtatcca
      541 acaggacgcg caggaattta tcgcttcgac catcgaattt gtgcgcggca tggtcaattc
      601 ggtcgttact tctctggaat ttgccgttgt tttgcggggc ttggctggca tcctgaccgt
      661 gttcggcttc gacattccgc acggcatcgt ttggtttgtc tatatatttg tgattttggc
      721 gacctttatc gccatgtgga taggcaaccc tttgattcgt tacaattatg aaaacgaaaa
      781 actcaacggc gactaccgtt attccctcat ccgcgtgcgc gaccacgccg aaagcgtggc
      841 gttttacagc ggcgaaaaac acgaacacga ccagctttct gaccgtttta aagccattat
      901 ccgcaaccgc tggcgtattg cgcgacaaag cgtctgcctg agcggcttta atgatatgtt
      961 caccaacggc atcaagctct tccccattat tttgcaagcg ccgcgcctgt ttgccggaca
     1021 aatcaaaatc ggcgacatcc agcagaccgt ccaagccttc gcgcgactgc aaaacgcgct
     1081 ttctttcttc cggatgttct acaacaaatt caccgcctac cgcgcccgat tggagcgtct
     1141 gtacggcttt ttgctgagta ccgaagaaca acacagcgcg cggcaacccg agattagcga
     1201 agtttcagac ggcattgcac tggaatgtaa ggcggcggca gcttttgcac gaattggcgg
     1261 cagcttttgc accgccgaag cgtaggaaat tgtattagct tttgcacaaa tttatccccg
     1321 tttaatggtc tgttaaacgg ggattatctt atgcctttaa aagtagtacg ctacttgcgg
     1381 tcagaactta taaatcaggt ggtcgtcgta gcttgaattg ctgttgatgt gctgctcaca
     1441 gtcaaggttt ggctgcctgc aaaacgctcc catccggtca ggcgcagttc tgccacgagg
     1501 tcaagataag caggcagttc gctgcgggtg ccggagaaga agacaaatgg cggccgcacc
     1561 attgccatca ggcgcagaaa ctctaccatg ccgaagtagt tcatcgcgtc cccttttccc
     1621 cggcggcgcg gctttggttg aaccgcgcgt cgtgctgcct gcctttcagt tcgctgatat
     1681 acgcctgccg gcagtggtgg cggtcgaaaa acacgccgtt gatcagggta tataaaatct
     1741 tccagcgggt tttgggcttt tgggcgagcc gcgcgcggcg ataggtgcgg cttgagaggg
     1801 tttcgtccgc cgcgccgccg gttagggcgt tgaacagttg gtcgatggct accaacaggt
     1861 ggtaaacgta ttttttcagc cgatgtttag cgtccattct tcgatttcct tttccagcgc
     1921 gtccaatccg ggcgcggttt cgatggtgtt caatttgtct tcgagctgct gacgctttcc
     1981 gataatcgcg ccggcggcaa cagccaggcg ggcggatttt tcgataactt tttcaatcaa
     2041 aacgtccaat tccacgcccc ttgcggcggc gatttgcgcc agcatcgggg tcggggcgtt
     2101 gttgtccgcc tgccgcgcga gggcttcttt ttcctgcctg taaaagctgt cgatttccac
     2161 ttggggatag cccgccaaga ggctgttttt gagttcgtcc gccttttccg cgaggcggaa
     2221 tgccaaggcg gttttttgtt tggcgaaacg ggcggcggcg gcggctttgc tgattttcca
     2281 ttttttgccg tcccattcgt ggtaatcgga cgggcgcggc ggggttaaaa cggggcggcc
     2341 gtcggaatct gcggcaatct gcccgccctg cgcctgtcct gccaaaaggg cggcgtattc
     2401 ttcggcgcgg acggcaaccg cgccttcggg gatgccgccc aatgtgtcgt cgtaaaagcc
     2461 gtttttgaaa tagatggtca ttttggggtt tccttttttg tatttacatt ttggtttttt
     2521 gccgcttata atgtaacgac aaaatacgct gcaagctgca atgaagattg aatttgactc
     2581 cgaaaaaaat caacgcaata tcgaagagcg gaatttgccc tttgaatcag tcgggcaaat
     2641 acgctggaca acggccgtta ttgtgcccga tgtccgcttt gattaccccg aaccgcgtta
     2701 tgttgccgct gcttatctag gcgatacgca acgcctgcac attgtctgtt ttacgccgat
     2761 aaaagacggc atccgtgtca tcagttttcg caaagcgaat aaaagagagg ttaaaaaata
     2821 tgcaacgtca atccttaaca aacgctgacg gcgaagtgcg ggaacttgcc gccgaagatt
     2881 ttgcgcttgc ccgccccatt gccgaagccc tgcccgaaga cttggcacag gtgctttttt
     2941 cccatcaaaa acaattggaa gaaaagggcg taatgcaaaa acgaaacaca ggcaaaaccc
     3001 cgaaacaact cgttaccata cgcctgtccg ccgatgtggt cgaaaaattc cgcgccggcg
     3061 gcaagggctg gcagacgcgc atcaacgagg ttttgcgcca atatatcgcc caattgaaat
     3121 aacgggggcg gccgctgctt atcgggcgcg cccgactatc ctttcccgcg cggtttcggc
     3181 cgtgcggttt tttgttgccg gtgccgtccg aagggggcag cggctttttg cgcggtcatt
     3241 cccgcctttc gttattcccg ccactttccg tcattcccgc gaaggcggga atcccgaaac
     3301 ccctgccgcc gtcattcccg cgcaggcggg aatccagtcc gttcggtttc agccgtttcc
     3361 gatagattcc tgccgcgttt ggggtctgga ttcccgcctg cgcgggaatg acggtggcgg
     3421 ggggtcggtt ttttccgata gattcctgcc gcgtttgggg tctggattcc cgcctgcgcg
     3481 ggaatgacgg cggcgggggt ttctgttttt tctgatagat tcctgccgcg tttgggggct
     3541 ggattcccgc tttcgcggga gtgacggtgg cgggggtttc ggtttttccg atagattcct
     3601 gttgcgtcgg ggggtctgga ttcccgcttt cgcgggaatg acgaaagcgg cgggaatgac
     3661 gaagagtggc aggattggcg gggcataggt ttcgggggat tggcggcgcg gccggtttcg
     3721 gtcggattgc ttggatcggc gggctgaagc ccatctctac aactcgtctc tacaacccat
     3781 ctctaca
//
LOCUS       DAWIVB010000176         3756 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWIVB010000176 DAWIVB010000000
VERSION     DAWIVB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.176"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(31..1941)
                     /locus_tag="WIX60_001816"
     CDS             complement(31..1941)
                     /locus_tag="WIX60_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ5306041.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSREDKIQTASAPKASDAEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINTQLSYPEIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKRNGNAV"
     gene            complement(1960..2604)
                     /locus_tag="WIX60_001817"
     CDS             complement(1960..2604)
                     /locus_tag="WIX60_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ5306042.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(2769..3587)
                     /gene="opc"
                     /locus_tag="WIX60_001818"
                     /gene_synonym="opcA"
     CDS             complement(2769..3587)
                     /gene="opc"
                     /locus_tag="WIX60_001818"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ5306043.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHISVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT          918 a          856 c          944 g         1038 t
ORIGIN      
        1 gtttgaacat ttttttcttc ctgtttgatt tcagacggca ttgccgttcc gtttggtttc
       61 cagcagctcc cagcgttcca gcttttccaa aagcagcatt tcgatttctt cggcgcggtt
      121 ttgcaatgca cctgcttttt cgtaatcttt gaaaatttca ggataggaaa gttgggtatt
      181 gatttcagcc tgctcggctt ccaaagcggc gatttcgtcg ggcagggcat cgagttcgcg
      241 ctgctctttg taggacagtt tgaccgtgcg gttggctttg ggtttttctt tggcgggttc
      301 ggcatcggat gctttgggtg cggatgccgt ctgaatttta tcttcccgcg attttgcgtc
      361 gatatagtcc tgatagccgc cgatgtattc tttcagacgg ccttgtcctt cgaaaacaat
      421 gctttgggta attacgttat caaggaacat acggtcgtgc gagacaagga atactgtgcc
      481 ttgataatcg cgcaacaggt cttcgagcag ctcttgggtg tcgatgtcta agtcgttggt
      541 cggttcgtcc aagaccagga tattggcagg acgggtaaag agttttgcca gcaaaaggcg
      601 gttgcgttct ccgccggaga gcgatgaaac agggctttgc gcacgggctg gatggaacag
      661 gaaatcttcc aaatagctca tgacgtgttt tttcttaccg ccgacttcaa cgtaatcgtt
      721 tccctgtccg agggtgtaaa acacggtgtc gttttcattc aacgcgctgc ggaactggtc
      781 gaaataggcg acttcctgct tactgccgat acggattctg ccgtaggtcg gctgcaattc
      841 gcccaaaatc agcttaagga aggtggtttt gccgatgccg ttggggccga ttaggccgat
      901 tttgtcgccg cgctgcaaga tagcggagaa tttgtccata atgactttgc cgccataggc
      961 aaacgaagcg tgttccaatt cggcgatgat tttgccactt ttctcaccgc tatcgagctt
     1021 gaagttgact tgtccctgta cgttgcggcg ttctgcacgc tggcggcgca gctcttccaa
     1081 acggcgcacg cggccttcgt tgcgggtacg gcgcgcttcg atgcctttgc gtatccatgc
     1141 ttcttcctgt gcgtggaatt tgtcaaagag gcggttgtgt tccgcttcga ctgccaactc
     1201 ttgcgctttt ttctcgctgt atttagagaa cgagccggga taggaacgca aaataccgcg
     1261 atcgagttcg acaatccgcg tggcgatatt gtccaaaaaa cggcggtcgt gggtaatcac
     1321 aaccaagctg ccttcaaacg ctttgagcag attttccagc caaataatcg cgtcgatatc
     1381 caaatggttg gtcggctcgt ccagcagcaa tacgtcgggc ttttgcaccc aagcctgagc
     1441 caaggcgacg cgctttttct gaccgccgga aaggttgccg attttttcat tttccggcaa
     1501 accgagttcc cccaaagtct gcttgactgc cgcatccagt ttccagccgt ccttcgcttc
     1561 gatttcaagt tgcaattcgt tgagttcttt caacaaagcc tcactcgaac cattttccaa
     1621 ctcatggctg acatgatgat aacggcgcaa taaatcacga atttcgccca aaccttcggc
     1681 aacggtatca aatacggttg cgtccttatc aaaaaaggat tcctgcggta cataaacgat
     1741 tttgaggttg ttttgaacaa taatctgccc gtcgtcgagc ttttgcaaac cggcgaggat
     1801 ttttaaaaac gaagacttgc ctgcgccgtt gcgtccgatt aagccgactt tttcgccgct
     1861 gtcgagttga aaagaagttt tgtcgagcaa ggcaacgtgg ccgatggcaa aagaagcgtt
     1921 ttctacagat aatatattca tgatacaaat tctcaacagt taccgtttgg attttaccgc
     1981 aagtttggcg cgggcaattt caaccgcacc cggcaggacg gaaacaataa tgatgccgcc
     2041 catcaccaag cccagattgt tttttacgac ggggaagttg gcaaagaaat agcccgcgta
     2101 agaaaacagg ataacccaca acaagccacc gatgatgttg tagcggataa atttggcata
     2161 gtgcattttc cccataccgg cgacgaaggg ggcgaaggtg cggacgatgg gcataaaacg
     2221 ggcaatgatg atggttttgc cgccgtgttt ttcgtaaaaa cggtgggttt tatcgagata
     2281 ttcacgtcgg aagattttag aatcggggtt ggcgaacagc ctgccgccga aatatttgcc
     2341 gacggtaaaa ttgagcgcgt cgccgagtat ggcggcaagg cttaataatg caaccatcaa
     2401 atgaatatcc ataccgccca gcgcggcaat cccgccggcg gcaaacagca gcgaatcgcc
     2461 gggcagtaag ggcgtaacaa tcaggccggt ttcgcaaaaa acaatcaaaa acagaatcgc
     2521 ataaatccac acaccgtatt gcgccgacag cgcgagcagg tgttggtcga tatggaggat
     2581 gaagtcgatg gcggaggcaa gcacggcgcg ttcctaaaaa aaacaaaccg cgtattttaa
     2641 ccgattggaa aaatgccgtc tgaaaagttt cagacggcat cggctattca aattcatttc
     2701 acgtaaaaac cgcaaaccaa aatagtttgc ggtttggcat ttaaagtgac aatgatgatt
     2761 tcaaatcatc agaattttat gccgacgcgc aagccgtatt cacgaatact ggttttcggg
     2821 atggtgagcg atacgtcgcc actcttggtt gttacactaa actcgccgga ttctttgtaa
     2881 gtgcgttgtt tgtagaacgg ccccgcctcg atgctggcgg attcgcccag ttttttacca
     2941 atatttgcac ccaatccaaa gccccagccg ttggtttttt gattgatgtc ttttttgaga
     3001 ttggtaacgc cggtgtttaa tttatagcgg gaattgaggt caaatttcac ttcagaccaa
     3061 gggttgatat accagccgtt acccagttgg gaaagcaaat ccgcgtgaac tttggctaac
     3121 cacgactgac ggctgctgtg aagcgtatgc ttggtggttt taatgctgtc ttttgaagat
     3181 tcaaaaccca agccggcacc cacacggaaa tttaaagaat cacttaacgt ttgggtgtag
     3241 gtgtagcctg tgtaaagatc gatacggttt tcaggaacgc cggtgggcag ttttacattt
     3301 ttagtcttac ccagcttgtt ctcatctgtt tccaaattaa taatattttt tttgctgcgc
     3361 ccgaaaccgg cttccaagcg gatgccttgg ttggcatcaa aaggaatatc agcacgcacg
     3421 ctgatgtgtt tggcagcttt gtgtttttct ttcaggaaag cacgagttga agaaatggaa
     3481 gagaggtcgg tgtggacggt aaactcatta gcggtttgaa gctcttgtgc agcggcggca
     3541 gtaccggtca gggcaatcat ggcacatgta aaaactgttt ttttcatagt taaaacctct
     3601 aaaatttgga ttgtagtcgg atatggtaac ataacgtaaa taatcgttac gcttacaatt
     3661 atattcttaa gctttcgggg ggggatttta catatattaa taaaaattaa caaatagtta
     3721 tttgtttaca acgaattgtt attctcactt ggtttt
//
LOCUS       DAWIVB010000177         3749 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWIVB010000177 DAWIVB010000000
VERSION     DAWIVB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.177"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            330..3578
                     /gene="nalP"
                     /locus_tag="WIX60_001819"
     CDS             330..3578
                     /gene="nalP"
                     /locus_tag="WIX60_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ5306044.1"
                     /translation="MRTTPTFPTKTFKPTAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLH
                     TGDFPNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYN
                     ENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDLVSHII
                     GGRSVDGRPAGGIAPDATLHIMNTNDGTKNEMMVAAIRNAWVKLGERGVRIVNNSFGT
                     TSRAGTADLFQIANSEEQYRQALLDYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLF
                     IFSTGNDAQAQPNTYALLPFYEKDAQKGIITVAGVDRSGEKFKREMYGEPGTEPLEYG
                     SNHCGITAMWCLSAPYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDN
                     LRTTLLTTAQDIGAVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFR
                     NDISGTGGLIKKGGSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNG
                     AASGGSLNSDGIVYLADTDQSGANETVHIKGSLQLDGKGTLYTRLGKLLKVDGTAIIG
                     GKLYMSARGKGAGYLNSTGRRVPFLSAAKIGQDYSFFTNIETDGGLLASLDSVEKTAG
                     SEGDTLSYYVRRGNAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPE
                     TVETAAADRTDMPGIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADM
                     QGRRLKAVSDGLDHNGTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENT
                     TAAATLGMGRSTWSENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRST
                     GADEHAEGSVNGTLMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGW
                     SGNSLTEGTLVGLAGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGK
                     TGARNMPHTRLVAGLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1016 a         1016 c         1003 g          714 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttcatcca
       61 ctataactag ggaaattcaa attaagttag aattatccct atgagaaaaa gccgtctaag
      121 ccggtataaa caaaataaac tcattgagct atttgtcgaa agttcaaatt tccattttaa
      181 aacaattagt aaaatcgagt ttatcctagt tgtccaagac aacccctata ataatataat
      241 tcaaaatata aaaatgggtt acatctaaac attacggaat ttttattccc tcgcctgaat
      301 tctattgtca gattcaagga gacctcatca tgcgaacgac cccaaccttc cctacaaaaa
      361 ctttcaaacc gactgccatg gcgttagctg ttgcaacaac actttctgcc tgcttaggcg
      421 gcggcggagg cggcacttct gcgcccgact tcaatgcagg cggtaccggt atcggcagca
      481 acagcagagc aacaacagcg aaatcagcag cagtatctta cgccggtatc aagaacgaaa
      541 tgtgcaaaga cagaagcatg ctctgtgccg gtcgggatga cgttgcggtt acagacaggg
      601 atgccaaaat caatgccccc cccccgaatc tgcataccgg agactttcca aacccaaatg
      661 acgcatacaa gaatttgatc aacctcaaac ctgcaattga agcaggctat acaggacgcg
      721 gggtagaggt aggtatcgtc gacacaggcg aatccgtcgg cagcatatcc tttcccgaac
      781 tgtatggcag aaaagaacac ggctataacg aaaattacaa aaactatacg gcgtatatgc
      841 ggaaggaagc gcctgaagac ggaggcggta aagacattga agcttctttc gacgatgagg
      901 ccgttataga gactgaagca aagccgacgg atatccgcca cgtaaaagaa atcggacaca
      961 tcgatttggt ctcccatatt attggcgggc gttccgtgga cggcagacct gcaggcggta
     1021 ttgcgcccga tgcgacgcta cacataatga atacgaatga tggaaccaag aacgaaatga
     1081 tggttgcagc catccgcaat gcatgggtca agctgggcga acgtggcgtg cgcatcgtca
     1141 ataacagttt tggaacaaca tcgagggcag gcactgccga ccttttccaa atagccaatt
     1201 cggaggagca gtaccgccaa gcgttgctcg actattccgg cggtgataaa acagacgagg
     1261 gtatccgcct gatgcaacag agcgattacg gcaacctgtc ctaccacatc cgtaataaaa
     1321 acatgctttt catcttttcg acaggcaatg acgcacaagc tcagcccaac acatatgccc
     1381 tattgccatt ttatgaaaaa gacgctcaaa aaggcattat cacagtcgca ggcgtagacc
     1441 gcagtggaga aaagttcaaa cgggaaatgt atggagaacc gggtacagaa ccgcttgagt
     1501 atggctccaa ccattgcgga attactgcca tgtggtgcct gtcggcaccc tatgaagcaa
     1561 gcgtccgttt cacccgtaca aacccgattc aaattgccgg aacatccttt tccgcaccca
     1621 tcgtaaccgg cacggcggct ctgctgctgc agaaataccc gtggatgagc aacgacaacc
     1681 tgcgtaccac gttgctgacg acggctcagg acatcggtgc agtcggcgtg gacagcaagt
     1741 tcggctgggg actgctggat gcgggtaagg ccatgaacgg acccgcgtcc tttccgttcg
     1801 gcgactttac cgccgatacg aaaggtacat ccgatattgc ctactccttc cgtaacgaca
     1861 tttcaggcac gggcggcctg atcaaaaaag gcggcagcca actgcaactg cacggcaaca
     1921 acacctatac gggcaaaacc attatcgaag gcggttcgct ggtgttgtac ggcaacaaca
     1981 aatcggatat gcgcgtcgaa accaaaggtg cgctgattta taacggggcg gcatccggcg
     2041 gcagcctgaa cagcgacggc attgtctatc tggcagatac cgaccaatcc ggcgcaaacg
     2101 aaaccgtaca catcaaaggc agtctgcagc tggacggcaa aggtacgctg tacacacgtt
     2161 tgggcaaact gctgaaagtg gacggtacgg cgattatcgg cggcaagctg tacatgtcgg
     2221 cacgcggcaa gggggcaggc tatctcaaca gtaccggacg acgtgttccc ttcctgagtg
     2281 ccgccaaaat cgggcaggat tattctttct tcacaaacat cgaaaccgac ggcggcctgc
     2341 tggcttccct cgacagcgtc gaaaaaacag cgggcagtga aggcgacacg ctgtcctatt
     2401 atgtccgtcg cggcaatgcg gcacggactg cttcggcagc ggcacattcc gcgcccgccg
     2461 gtctgaaaca cgccgtagaa cagggcggca gcaatctgga aaacctgatg gtcgaactgg
     2521 atgcctccga atcatccgca acacccgaga cggttgaaac tgcggcagcc gaccgcacag
     2581 atatgccggg catccgcccc tacggcgcaa ctttccgcgc agcggcagcc gtacagcatg
     2641 cgaatgccgc cgacggtgta cgcatcttca acagtctcgc cgctaccgtc tatgccgaca
     2701 gtaccgccgc ccatgccgat atgcagggac gccgcctgaa agccgtatcg gacgggttgg
     2761 accacaacgg cacgggtctg cgcgtcatcg cgcaaaccca acaggacggt ggaacgtggg
     2821 aacagggcgg tgttgaaggc aaaatgcgcg gcagtaccca aaccgtcggc attgccgcga
     2881 aaaccggcga aaatacgaca gcagccgcca cactgggcat gggacgcagc acatggagcg
     2941 aaaacagtgc aaatgcaaaa accgacagca ttagtctgtt tgcaggcata cggcacgatg
     3001 cgggcgatat cggctatctc aaaggcctgt tctcctacgg acgctacaaa aacagcatca
     3061 gccgcagcac cggtgcggac gaacatgcgg aaggcagcgt caacggcacg ctgatgcagc
     3121 tgggcgcact gggcggtgtc aacgttccgt ttgccgcaac gggagatttg acggtcgaag
     3181 gcggtctgcg ctacgacctg ctcaaacagg atgcattcgc cgaaaaaggc agtgctttgg
     3241 gctggagcgg caacagcctc actgaaggca cgctggtcgg actcgcgggt ctgaagctgt
     3301 cgcaaccctt gagcgataaa gccgtcctgt ttgcaacggc gggcgtggaa cgcgacctga
     3361 acggacgcga ctacacggta acgggcggct ttaccggcgc gactgcagca accggcaaga
     3421 cgggggcacg caatatgccg cacacccgtc tggttgccgg cctgggcgcg gatgtcgaat
     3481 tcggcaacgg ctggaacggc ttggcacgtt acagctacgc cggttccaaa cagtacggca
     3541 accacagcgg acgagtcggc gtaggctacc ggttctgacg gacaggaagc agacagccgc
     3601 aaagatcacg gtctttgcgg ctgtttctta tgaaaagaaa accctattcc aattgcctgc
     3661 ttctattgtt tcaagacttc ttccaaagat tcggcattaa tcagatgtat agcggattaa
     3721 caaaaatcag gacaaggcgg cgaagccgc
//
LOCUS       DAWIVB010000178         3717 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWIVB010000178 DAWIVB010000000
VERSION     DAWIVB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.178"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(225..548)
                     /locus_tag="WIX60_001820"
     CDS             complement(225..548)
                     /locus_tag="WIX60_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ5306045.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(628..1284)
                     /locus_tag="WIX60_001821"
     CDS             complement(628..1284)
                     /locus_tag="WIX60_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ5306046.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDVEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1387..1914)
                     /locus_tag="WIX60_001822"
     CDS             complement(1387..1914)
                     /locus_tag="WIX60_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ5306047.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAV"
     gene            2069..2842
                     /locus_tag="WIX60_001823"
     CDS             2069..2842
                     /locus_tag="WIX60_001823"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ5306048.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRIHPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPNNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2849..3199
                     /gene="secG"
                     /locus_tag="WIX60_001824"
     CDS             2849..3199
                     /gene="secG"
                     /locus_tag="WIX60_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ5306049.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3213..3298
                     /locus_tag="WIX60_001825"
     tRNA            3213..3298
                     /locus_tag="WIX60_001825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3247..3249,aa:Leu,seq:gag)
BASE COUNT         1012 a         1170 c          790 g          745 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa actttcagtt tgcccgcctc gtcctagccc
       61 tctcccacgg gagaggggat tggggtgcag gctgccttta aggttcaggc aaattttaac
      121 ttcgttgata ccgcgcttta gcttcgcaga agctgcactt tcagacgacc ttttggaaca
      181 ccacaggtac acgcatttaa ggaatgccgt ctgaaatgcc tgcctcaata acgcatcatg
      241 ttgccgtcaa tctcggccgc ccatgcatcg atgccgccct gaaggttgta caggttttca
      301 aaacccgcgt ccgccaaata catcgccgta tgcaggctgc ggataccgtg gtggcaatac
      361 accacaagcg gcacatcatc cggcagctcg ttctgccgca gcggaatcag attcatcggg
      421 atatgcagcg catttggcag cgaacaaacc gccgtttctt catccgtacg cacgtccaac
      481 aaacaaaaca tccgcccttc gtccatccac gctttcaatt ccgcgggccc aagttgcaca
      541 atatccatcg caccccccaa aaaaaaccaa gcaaaatgcc gtctgaagcc ccaaacccgc
      601 tttcagacgg catgacctgt caacatctta aaaatcgaaa ccgccaaacg gatcggcatc
      661 cttatcatcc aaatgcgcca ccaaggtatc gaacagcacc ttctcttcaa acacatcgcc
      721 cctgcgcgta atcaaaagcg cgcgttgaac aggcttgcga cctacgataa ccaccatgcg
      781 tccgccatct ttcaactgtt ctttcaacac ttcaggcaca aggtttaccg caccgccgac
      841 ataaaccgca tcaaacggcg cacctgcgga aagttcggtc aacccgttgt tttgcacata
      901 atcgatattg tccaaaccca agccgtccaa caccgctttg gcgcggtttt gctgttcgac
      961 atcgatgtcg tccgacacca cacgaccagc caattttgcc aacagcgcgg tcgcatagcc
     1021 cgaacccgtg ccgatttcca aaaccgtatc gtttttcgtc agcttcaagc cctgcgccag
     1081 ccgcgccacg actttcggct cgagcatctt atgaccgttg gcaagcggca gcgccatatc
     1141 cgcatacgcc aaaccctgca agtcctcatc gacaaaaagc tcgcgcggaa tctccgccaa
     1201 agcgtccaac acatcaaaat ccaatacatc ccacggacgg atttgctgtt cgaccatatt
     1261 gaaccgcgct ttttcaaaat ccatttagtg ctccgtcaaa taattgttcc aacaatggca
     1321 ggcattataa aaccacactc ccgccgcgcc aacttcggca cgcgccgaca gcccgttttg
     1381 tcagtctcaa accgcctgac gcgaagcctc aaaccgcttc tccaaaatct tcgccagttc
     1441 gcccaaatac aaagcatccg tctcatcaaa ctgcgccaaa tgttcgctgt ccgcgtccaa
     1501 cacgccgata cagcggccgt ctgaaaacag cggcacgaca atctccgaac gtgacaaaga
     1561 cgaacaggca atatggtcgg gatgcgcgtt gacatcctta acaaccaccg tttcaccctt
     1621 cgcccaagcc tgaccgcaca ccccgcgacc gaacggaatc cgcgtacacg ccaaaggccc
     1681 ctgaaacggt gccaaaacca attcgtccga acgcgtatcg accaaataaa aacccaccca
     1741 aaaccaaccg aacgcctcct tcaaaaccgc cgccgtgttc gccaaattcg ccacccaatc
     1801 cgcctcgtca gccaccacag actcaatctg cggcaacacc tcccgataaa gcgcggcctt
     1861 gtccgaagcc gaaaaatgaa gcgcgtgcat cacatctcct atagttgcat acatatcagg
     1921 cggccattat aaaacagcct gcccgaaaca acattccaaa ccgcccggcc ggccgcttca
     1981 agttgcgaac ccgccgcata tccactaaac ttcacgttgc accgcgccac acgcggcaga
     2041 caaaaaaaca cgacacggag caaaaaagat gtatcgccaa atcggaatgt gggatcaaaa
     2101 atgggtcatc ggcaactgga aaatgaacgg ccggctccaa aacaacaacg cactgatgca
     2161 ccgcttccgc atccacccca ccgccgaacg cgtcctcatc ggactcgccg ccccgaccgt
     2221 ttacctgctg caactgcaca acgccatgca aatcgtttta aacaaccgca tcctcacctg
     2281 cgcccaagac gtgagccgct tccccaataa cggcgcgtac accggcgaag tgtccgccga
     2341 aatgctcgcc gacaccggca cagacatcgt cctcatcgga cactccgaac gcagccttta
     2401 tttcggcgaa aaaaacgaaa tccaacgccg caaaatggaa aacgtcctca acgtcggact
     2461 catcccgtta ttgtgcgtcg gcgaaagcct cgaagagcgc gaagccggca aagaacacga
     2521 agtcatcgcc catcagcttt ccatcctgca agggctggat accaaaaaca tcgccgtcgc
     2581 ctacgaaccc gtctgggcga tcggcaccgg caaagtcgcc accgtcgaac agattgccga
     2641 tatgcacgca ttcatctaca aagaaatctt gtctttgtgc ggaagcgatg ttaaaatccg
     2701 tgtcctttac ggcggaagtg tgaaagcgga caacgcggcc gacatcttcg cagtacctta
     2761 tgtggacggc gcactcgtcg gcggcgcgtc attgtcgtac gactccttta ccgccatcat
     2821 cagtgccgca caaaatgcgt agaaaaatat ggaagccttc aaaaccctaa tttggattgt
     2881 taatataatt tccgctttgg ccgtcatcgt gttagtattg ctccaacacg gcaaaggcgc
     2941 ggatgccggc gcgactttcg gatcgggaag cggcagcgcg caaggcgtat tcggctctgc
     3001 cggcaacgct aacttcctca gccgctcgac cgccgttgca gcaacatttt tctttgcaac
     3061 ctgcatggct atggtgtata ttcacaccca cacgacaaaa cacggtttgg acttcagcaa
     3121 cgtacaacaa actcagcaag cacccaaacc cgtaagcaat accgaacctt ctgcccctgt
     3181 tcctcagcag cagaaataac agtttttcaa atgccgacat ggtgaaattg gtagacacgc
     3241 tatcttgagg gggtagtggc cgtaggctgt gcgagttcaa atctcgctgt cggcaccaac
     3301 acacaaaaac gcctgaaaat ttcaggcgtt ttttgttcaa tcccatccaa cgctgtcaac
     3361 caattccaat caatacaagg atttcaggct gattgttatc ctgccgtccc ccttcctgac
     3421 agtgcaatcc cgtccaatcc gccctaattg aagtaaccta aaatttacgg tatcttttgc
     3481 ggtatctgaa aaatacctcg aaaaaatacc gcaaaaataa agctgaacga ccgccaaatc
     3541 aggaatgcca agcggaaaag agcttgcggg gaatactgcc aagacgtagg gaacaagggg
     3601 gaaaccgtcc aagatgcagg gcggtttttt ttgggttttt ggaaaaaacc tatactagga
     3661 agcgataccc ttagttgtta ccttgttacc ggggaaaagt tagataaata agcatat
//
LOCUS       DAWIVB010000179         3708 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWIVB010000179 DAWIVB010000000
VERSION     DAWIVB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.179"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(153..398)
                     /locus_tag="WIX60_001826"
     CDS             complement(153..398)
                     /locus_tag="WIX60_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306050.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            510..938
                     /locus_tag="WIX60_001827"
     CDS             510..938
                     /locus_tag="WIX60_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ5306051.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            950..1426
                     /locus_tag="WIX60_001828"
     CDS             950..1426
                     /locus_tag="WIX60_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ5306052.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1776..2264)
                     /locus_tag="WIX60_001829"
     CDS             complement(1776..2264)
                     /locus_tag="WIX60_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ5306053.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WIX60_001830"
     CDS             complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WIX60_001830"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ5306054.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAEHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAS"
BASE COUNT          837 a          972 c          872 g         1027 t
ORIGIN      
        1 ccgcctgcgc gggaatgacg gctcaaaagt tacgaaacga aaaataaccg aaaccaaaca
       61 agtcggattc ccgcgcgggc gggaatgacg ataagcgtaa tttcaatctg ccatattgtc
      121 ttttcacttg cctgaaaaca gatttcagcc acctatccct gcatccggcg gctgatgtag
      181 tcggcaatca aatcccgctt ttcccaaaaa aacttctgca ttttttgttc ggaatacagt
      241 ttgcggacca gagtgggcaa accgctgtgg acgatggcgg cgatttcttt gttgcctttt
      301 tcttcctgca ttgccgtctg aaaatccata cccctgcctt tcagatattc tgcaaggcgg
      361 ttgcggatgt gggaattgag ttcgatggat ttgagcattt tgcctccgtt ttgcggtaag
      421 gatttattcg atttgtttat ccgcctgatt ttatcacgtt tcccaagcgg tttgcctgta
      481 caataccgct tttcggcagg gtgtgggcga tgatgttttc ttggttcaag ctgtttcact
      541 tgttttttgt catttcgtgg tttgcagggc tgttttacct gccgaggatt ttcgtcaata
      601 tggcgatgat tgatgtgccg cgcggcaatc ccgagtatgt gcgtctgtcg ggcatggcgg
      661 tgcggctgta ccgttttatg tcgccgttgg gcttcggcgc ggtcgtgttc ggcgcggcga
      721 taccgtttgc cgccggctgg tggggcagcg gctgggtaca cgtcaaactg tgtttgggct
      781 tgatgctctt ggcttaccag ttgtattgcg gcgtgctgct gcgccgtttt caggattaca
      841 gcaatgcttt ttcacaccgc tggtaccgcg tgttcaacga aatccccgtg ctgctgatgg
      901 ttgccgcgct gtatctggtc gtgttcaaac cgttttgacg gagcatctta tgccgataga
      961 aatcgaacgc cgttttttga ttggaaacga caactggcgg caatacgccg acgaaccgct
     1021 gctgttgagg cagggttatc tgtctgttga aaaagaacgc accgtccgtg tccgcattgc
     1081 cggaaaacag gcgtggctga cgctgaaagg ctatatttcg gaaatcagcc gcagtgagtt
     1141 tgaatacgaa atcccgcttg ccgatgcgga aaagatgatg gaaacgatgt gcccgtttaa
     1201 aatggaaaaa tggcgttatc cggtcagatg gggcggcagc ctgtttgaaa tcgatgtttt
     1261 tcttggcgat aatgcgcctt tggtcgtcgc ggaaatcgag ttgcccgacg agaatgccga
     1321 ttttgaccgt ccggactggc tgggacggga aatcactgcc gacggtatgt ttaccaatgc
     1381 ttatttgagc aggcatccgt tctcaagctg gaaaaatgcc gtctgaaacg gcttcagacg
     1441 gcattctccg acaaccatcg caaatccaaa gacagcctct atatccttat ttctgctttc
     1501 tttgttccat tattcccaat aaatccccat aacctaaaat ctcgtcattc ccgcgcaggc
     1561 gggaatccgg ttctttaaac ttcggttttt tccgataaat tgctttagct tttcgtttcc
     1621 ggattcccgc ttttgcggga atgacgatgg aagattgttg ttatttcgga taaattaccc
     1681 taactttaaa atcccggatt cccatcttcg ggggaataac aatattttct atttcagccc
     1741 atcatttgcg ggggatttta tagagagttt atatttcaat atctcaaata atgcacaaaa
     1801 gcatagcgcg tgcctttgct gctgacacgg cgttccgtcc gctctgcttc tttccaatgc
     1861 gtccggtcta ttgcggggaa aaatgcatct ccttccacag acaaatccac ttcggttatc
     1921 cgcaaatcgg tcgccaatgg catcgcttgt ccgtatatct gcgcgccgcc cataatgacg
     1981 gcttcttccg cgcctgcgca caatgccaat gccgcctcca aacttgccgc cgtttccgcg
     2041 cctgccgcgc aataatccgc ctgccggctg atgacgatgt tcctccgtcc gggcaggggt
     2101 ttgacgggca gggattccca cgttttccgc cccataatga cgggtttgcc caaggtatag
     2161 gctttgaaaa atgcgaaatc ttcggggatg tgccaaggca tagcattgcc cgccccgatg
     2221 cacaggtttt ccgcacacgc cgcaattagg gttattttga gcatatttcc tccacaaaaa
     2281 tgccgtctga acgcttcaga cgacatttta tgacattctg cgtcaaaaat ctcaactggc
     2341 gcgcgccata cgttttttgt ttgcacctgc caacaatgcc aacagttctt cagtcgcgcc
     2401 ccaaccgata cacgcatcgg taatgctctt gccgtacact tccggcttgt cctgtctgcc
     2461 ttcgaccaaa tggctttcca ccatcacgcc catgatattg ccgccgtcct gttccaattg
     2521 ggcggcaatg tcttgtgcca cttccatctg ccgagtgtaa tccttgcggc tgttggcgtg
     2581 gctgcaatct atcatcagct tgtcggttac ccctgccgca cgcagttgtt ccgccgcctc
     2641 gctgacgtgt tccgcatcat aattcggctc tttgccgccg cgcaaaatga catgacagtc
     2701 gggattgccg ccggtatgga caatggcgga atgcccggcc ttggttacag acaggaaatg
     2761 atgcgaatgg ctcgccgcac cgattgcgtc gatggcaatc ttcaaattgc cgtccgtacc
     2821 gtttttaaag ccgacggggc aggacagccc gcttgccaat tcgcggtgaa cttggctttc
     2881 ggtcgtccgc gcaccgattg ccccccaaga aatcaagtcc gcataatatt gcggcgtaat
     2941 catatccaaa aactcggtag aggcaggcat acccatattg ttcagcgaca acaacaggct
     3001 gcgcgcctga cgcaaaccga aattgatgtc aaacgtaccg tccaaatgcg ggtcgttaat
     3061 caaacctttc caacccaccg tcgtcctcgg cttctcgaaa taaacgcgca tcacaatcaa
     3121 aagctcgttt tcatactgct tgcggagttt caacaaacgc tccgcatatt ccaacgccgc
     3181 tttcggatcg tgaatcgaac acggcccgat aataaccaac agccgcttgt ccctgccgtg
     3241 aaccaaatcg gaaatttcct gacgggtgcg gtgaaccaag cccgaagcct ctttggaaat
     3301 cggcagctcg taaagatggg ctatcggcgg caacaactct ttaacttctt taatcttaat
     3361 atcgtctgtg gggtaatggt gtgtcatctt gcatctttcc tgatgggttt ttatacgcgg
     3421 cttaggttac atcgtttacc ttgcacaaac aagtgcaaca tattactaca aagctatttt
     3481 atttcaaaat aactgatata tcttttttta tagattttaa atttcatatt tttattattt
     3541 tagaaatttt atttcaaata aaaagaaaca gattgataaa aacaataccc gccattcatt
     3601 tggacaaacc tgcacggaac atccgttttc ccatttccac gacaatccgg tacagaacac
     3661 ccccgctgcc gattccgcct tcacatccgg caaaatttca tacacaag
//
LOCUS       DAWIVB010000180         3691 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWIVB010000180 DAWIVB010000000
VERSION     DAWIVB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.180"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            60..689
                     /locus_tag="WIX60_001831"
     CDS             60..689
                     /locus_tag="WIX60_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ5306055.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            864..3239
                     /gene="hmbR"
                     /locus_tag="WIX60_001832"
     CDS             864..3239
                     /gene="hmbR"
                     /locus_tag="WIX60_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HEZ5306056.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVLAADEAATETTPVKAEIKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIEIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLDDRQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGNRGYAVEGEGSGANIRGSARGIPDSSKHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLTASSWREADDVNRRRNANLFYEWMPDSNWLSSLKADFDYQKTKV
                     AAVNNKGSFPMDYSTWTRNYNQKDLDEIYNRSMDTRFKRFTLRLDSHPLQLGGGRHRL
                     SFKTFVSRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSSR
                     AGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWRVGYDITSG
                     YRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGMLDANLYQSNYRN
                     FLSEEQKLTTSGTPGCTEENAYYGICSDPYKEKLDWQMKNIDKARIRGIELTGRLNVD
                     KVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLTY
                     LGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAGV
                     YNLFNRKYTTWDSLRGLYSYSTTNAVDRDGKGLDRYRAPGRNYAVSLEWKF"
     gene            complement(3447..3569)
                     /locus_tag="WIX60_001833"
                     /pseudo
     CDS             complement(3447..3569)
                     /locus_tag="WIX60_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
BASE COUNT          997 a          931 c          934 g          829 t
ORIGIN      
        1 gggcaagtgc ggattttgcc cgcaccgcat ccttatccct taccgacaaa ggaaaaaata
       61 tgagtgaaac cgaaaatcaa gcattgacat ttgccaaacg cttgaaggcg gataccacgg
      121 cggttcacga cagcgtggat aacctcgtta tgtctgtcca accgtttgtc agcaaagaaa
      181 actacatcaa atttttgaaa ctccaatccg ttttccacaa ggctgtcgac cacatctata
      241 aagatgccga attaaacaaa gccattcccg agctggaata catggcgcga tacgatgccg
      301 taacgcaaga ccttgcagat ttgggtgaca aaccttacga atacggcaaa ccgctgccgc
      361 atgaaaccgg caacaaagca atcggctggc tttattgcgc cgaaggatcc aatttgggcg
      421 cggcattttt gttcaaacac gcccaaaaac tcgattacaa cggcgaacac ggcgcgcgcc
      481 acctcgcacc ccatcccgac gggcgcggca aacactggcg cgctttcgtc gagcatctga
      541 acgctttgaa cctgactccc gaagccgaag cggaagccat ccaaggcgcg cgcgaagcct
      601 ttgcattcta caaagtcgtg ttgcgcgaaa ccttcggctt ggcagccgat gccgaagcac
      661 cggaaggaat gatgccgcac aggcactaaa aaataatcga accaaataaa caaggtctcg
      721 gcatagctgt ttgcagggac ctttaattac acggcgcggc tttgtttaca tggattactg
      781 tcttattaaa tattaatgat tatcataaaa tctattattc gctaaccgat ggatgaacaa
      841 tccatacatc ttgagttgat aatatgaaac cattacaaat gctccctatc gccgcgctgg
      901 tcggcagtat tttcggcaat ccggtcttgg cagcagatga agctgcaact gaaaccacac
      961 ccgttaaggc agagataaaa gcagtgcgcg ttaaaggtca gcgcaatgcg cctgcggctg
     1021 tggaacgcgt caaccttaac cgtatcaaac aagaaatgat acgcgacaat aaagacttgg
     1081 tgcgctattc caccgatgtc ggcttgagcg acagcggccg ccatcaaaaa ggctttgctg
     1141 ttcgcggcgt ggaaggcaac cgtgtcggcg tgagcataga cggtgtaaac ctgcctgatt
     1201 ctgaagaaaa ctcgctgtac gcccgttatg gcaacttcaa cagctcgcgt ttgtctatcg
     1261 accccgaact cgtgcgcaac atcgaaatcg tgaagggcgc agactctttc aataccggca
     1321 gtggtgcatt gggcggcggt gtgaattacc aaacgctgca aggccgtgat ttgctgttgg
     1381 acgacaggca attcggcgtg atgatgaaaa acggttacag cacgcgtaac cgtgaatgga
     1441 caaatactct cggtttcggt gtgagtaacg accgcgtgga tgctgctttg ctgtattcgc
     1501 aacgtcgcgg tcatgaaacc gaaagtgcgg gaaaccgagg ctatgctgtg gaaggggaag
     1561 gcagtggcgc gaatatccgt ggttcggcac gcggtatccc tgattcgtcc aaacacaaat
     1621 accacagctt tttgggtaag attgcttacc aaattaacga taaccaccgc atcggcgcat
     1681 cgcttaacgg ccagcaggga cataattaca cggttgaaga gtcttataac ctgaccgctt
     1741 cttcctggcg cgaagccgat gacgtaaaca gacggcgcaa tgccaacctc ttttacgaat
     1801 ggatgcctga ttcaaattgg ttgtcgtctt tgaaggcgga cttcgattat cagaaaacca
     1861 aagtggcggc ggttaacaac aaaggctcgt tcccgatgga ttattccacc tggacgcgca
     1921 actataatca gaaggatttg gacgaaatat acaaccgcag catggacacc cgattcaaac
     1981 gttttacttt gcgtttggac agccatccgt tgcaactcgg gggggggcga caccgcctgt
     2041 cgtttaaaac tttcgtcagc cgccgtgatt ttgaaaacct aaaccgcgac gattattact
     2101 tcagcggccg tgttgttcga accaccagca gtatccagca tccggtgaaa accaccaact
     2161 acggtttctc actgtctgac caaattcaat ggaacgacgt gttcagtagc cgcgcaggta
     2221 tccgttacga ccacaccaaa atgacgcctc aggaattgaa tgccgagtgt catgcttgtg
     2281 acaaaacacc acctgcagcc aacacttata aaggctggag cggttttgtc ggcttggcgg
     2341 cgcaactgaa tcaggcttgg cgtgtcggtt acgacattac ttccggctac cgtgtcccca
     2401 atgcgtccga agtgtatttc acttacaacc acggttcggg taattggctg cccaatccca
     2461 acctgaaagc cgagcgcagc accacccaca ccctgtctct gcaaggccgc agcgaaaaag
     2521 gcatgctgga tgccaacctg tatcaaagca attaccgcaa tttcctgtct gaagagcaga
     2581 agctgaccac cagcggcact cccggctgta ctgaggaaaa tgcttactac ggtatatgca
     2641 gcgaccccta caaagaaaaa ctggattggc agatgaaaaa tatcgacaag gccagaatcc
     2701 gcggtatcga gctgacaggc cgtctgaatg tggacaaagt agcgtctttt gttcctgagg
     2761 gttggaaact gttcggctcg ctgggttatg cgaaaagcaa actgtcgggc gacaacagcc
     2821 tgctgtccac acagccgctg aaagtgattg ccggtatcga ctatgaaagt ccgagcgaaa
     2881 aatggggcgt attctcccgc ctgacctatc taggcgcgaa aaaggtcaaa gacgcgcaat
     2941 acaccgttta tgaaaacaag ggctggggta cgcctttgca gaaaaaggta aaagattacc
     3001 cgtggctgaa caagtcggct tatgtgtttg atatgtacgg cttctacaaa ccggctaaaa
     3061 acctgacttt gcgtgcaggc gtgtacaacc tgttcaaccg caaatacacc acttgggatt
     3121 ccctgcgcgg tttatatagc tacagcacca ccaatgcggt cgaccgcgat ggcaaaggct
     3181 tagaccgcta ccgcgcccca ggccgcaatt acgccgtatc gctggaatgg aagttttaat
     3241 ctggtattat tgaattaatc gccttgttga aaattaaagc cgtccgaatt gtgttcaaga
     3301 attcattcgg acggcttttt accgtaaatc tgtgtgatgg ggttttatat tgatacagta
     3361 tccgaactca ggcagggggt aatgttgcag gattaaaaat gccgtctgaa aaatgtttca
     3421 gacggcattt tttggttgcc gatatgttaa cggacgacgt tggcgcgcat gaccacgcgg
     3481 acgtggaacg agcctgcctg acagtcgtat tgtccgcctt gcagcggctg cttgtcgaag
     3541 tagctgtgga agccggtaac gccgcgtccg caagggtggc ggcagatgcc gcattgtgtt
     3601 tataatgccg tgtcagacgt ttgatttatg ggaaaataat gatgcttacc aatcaggaag
     3661 taaccggtgt caaactggga taccgcaaag c
//
LOCUS       DAWIVB010000181         3687 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWIVB010000181 DAWIVB010000000
VERSION     DAWIVB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.181"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..162
                     /locus_tag="WIX60_001834"
     CDS             <1..162
                     /locus_tag="WIX60_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ5306057.1"
                     /translation="DIWLHNGCLKISPSRHVKLEAWNAIDADDVILSLDNSPEEIGAG
                     LKLALSHCR"
     gene            175..362
                     /locus_tag="WIX60_001835"
                     /pseudo
     CDS             175..362
                     /locus_tag="WIX60_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(444..824)
                     /locus_tag="WIX60_001836"
     CDS             complement(444..824)
                     /locus_tag="WIX60_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ5306058.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1301..3382
                     /locus_tag="WIX60_001837"
     CDS             1301..3382
                     /locus_tag="WIX60_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ5306059.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPDRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3372..3566
                     /locus_tag="WIX60_001838"
     CDS             3372..3566
                     /locus_tag="WIX60_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ5306060.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          836 a         1064 c          944 g          843 t
ORIGIN      
        1 gatatttggc tgcacaacgg ctgcctgaaa atcagcccga gccgccatgt caagctggaa
       61 gcgtggaatg ccattgatgc agacgatgtc attttatcat tggataacag ccctgaagaa
      121 atcggagcag gtttaaagtt ggcattgagc cactgccgat aatatttgac aaaaggccgt
      181 ctgaaaaaca gctttgacaa agacgcggtt gccaaagaga tcgacctaca aagggaagta
      241 acgcaggcgt tcggcaaaac gccgcccaag ccgcagcggc cgttgccgac gaactcggca
      301 atacccgaag ttacggacgg tatcaggcag cccgaaccct gccggaggcc gaactgcaaa
      361 acaaatccga cacaaaaaac cgtccgtccg acacaaaaaa ccgcccgaaa aatctggacg
      421 gcggttcaaa caggctgccc cgtttaacgg gcgcggcagg aagtttcgac cgaattgccg
      481 taggcatcgg taaagccgaa aaaggcttcg ccgcctttct ggtgccactc ggttgcgttt
      541 ccgaacaaac cgtgttcggc ggtatagcgt tcgccggatg cggcaacgtc ggaagagagg
      601 acggcacgcc tgccgtccag ccgcaacgcg actttgccgc tgtccaaatg gcggacgcgc
      661 acagacaaac cgttctcgca ggaaaacgcc cgaaaatcgt ccgtgccggc ttggttttga
      721 acgggcggca tatgcccgcg tccgccgtca tcatacgcct ccggcacggc acaggccgcc
      781 aaagacaaaa ccggtacggt cagcgcgaaa aacctgatat tcataaaagc tccccaataa
      841 aaataagata tgaaacaacc gccctgattc caagctgcgg caacgccata ctataaacgg
      901 acgcgcaaac acacaagccc gataaccgga atttacctgc gatgaatcaa taatccggat
      961 tgcgcgcccc ttctttaccc ctcttccgat gccgcctttt gcctgacgat gccgtctgaa
     1021 cctgcctgcc cgcccggagg aatgtaaatt ttttccaaat tccaagtaaa aaccgctatc
     1081 ggtgtgctaa tttgcgttaa aatcctattc ggcgtttaac gttttgtgcg cccgcatccc
     1141 tgcactgttt gatgcgggca taaggcacaa atcccgacaa gcgcactgtt tcatacttcg
     1201 tcaatcattc agactccggt ttgtgcccgt gccggcagat ggttcggccg tttcccgccg
     1261 ttcaggcata ttccgacagt gtgagataag gatttattcg atgaaatcac tcaaaacctt
     1321 cctcatttgg ggcatagtgg tactggtcgg cttagcatcc tttaccactc tggccctcag
     1381 ccgaggcgaa caggtcagcg cggtatggat ggtcaccgcc gccatatccg tttactgcat
     1441 cgcctaccgt ttttacagcc tctacatcgc caaccgcgta atgcggctcg atcctgaccg
     1501 cctgactccg gcagaacgcc acaacgacgg cttggactac gttccgacgc acaaaggcgt
     1561 attgttcgga caccactttg ccgcaattgc cggcgcgggc cctttggttg gtccggtttt
     1621 ggcggcgcaa atgggttatc tgcccggtac tttgtggatt atcttcggcg tggtatttgc
     1681 cggcgcggta caggatatga tggtcttgtt cgtctctatg cgccgcgacg gtaagtcttt
     1741 gggcgatatt gtgaaacagg aactcggcac tgtccccggc gtgattgcct ccatcggtat
     1801 tttgatgatt atggtcatca ttatggcggt gttggcgttg attgtcgtaa aagcattggt
     1861 tcacagccct tggggtacgt tcaccattgc agcaactatg ccgattgcgc tgtttatggg
     1921 tatttacacg cgttatatcc gtccgggcaa aatcggcgag atttccatcg tcggctttat
     1981 tttgctgatg ctggcggtaa tttacggcga agatgtggct aaaagttcca tcgggcattg
     2041 gttcgacctt gacggcatcc agctcacttg ggcgattatg atttacggct ttgtcgcctc
     2101 cgtattgccc gtatggttgc tgctcactcc gcgcgactat ctctccacct tcctgaaaat
     2161 cggtacgatt gcggccttgg ctttgggtat cgtcatcgtc aatcccgctt tgcaaatgcc
     2221 tgccgtaacc cactttatcg acggttcggg tccggtattc tcaggcgcat tgttcccatt
     2281 cttgttcatt accatcgcct gcggtgcggt ttcgggcttc cacgcgctga tttcttccgg
     2341 cactacgccg aaaatgctgg aaaacgaaac ccacgtccgc atgatcggtt acggcggtat
     2401 gttgatggaa agtttcgtag ccattatggc acttgccgct gccgcatcgc ttgatcccgg
     2461 cgtgtacttc gccatgaaca gcccagccgc cctgatcggt acggatgcca ataccgccgc
     2521 cgaagtgatt accaccaagc tgcaattccc tgtcgatgcc gcaaccctgt tgcacactgc
     2581 taaagaagtc ggcgaaaaca ccatcctttc ccgtgccggc ggtgcgccca ccctcgcagt
     2641 cggtatggcg cacattatga gccgcctgat tccgggcgag gcgatgatgg cgttctggta
     2701 tcacttcgcc ctgttgtttg aagccttgtt catcctgacc gccgtcgatg ccggtacgcg
     2761 cgtcgcacgt tttatgattc aagacttggg cagcatcttc tacaaacctt tcggcaacac
     2821 cgactccatc cccgccaacc tgattgcgac cttcttcgcc gtggcattgt ggggctactt
     2881 cctctacacg ggcgtgaccg acccgttggg cggcatcaac tcgctctggc ctttgttcgg
     2941 catcgccaac caaatgctgg caggcgtagc cttgattatg tgcgccgtgg tgctgattaa
     3001 gatgaaacgc gaccgttatg tctgggtggt actcgttccc gccgtcggcg tactgttcgt
     3061 aacctgctac gccggcctgc aaaaactgtt ccacagcgac ccgcgcatca gcttccttgc
     3121 ccacgccggc aaatacagcg acgcattggc taaaaacgaa atccttgcgc ctgccaaaga
     3181 catcggcgaa atggcgcaaa tcatcttcaa cgacaagatt aatgccggtc tgaccatcct
     3241 cttcttgtcg gttgtcgtga ttgtcgccgc gtacggtttg cgtaccgccc tcaaagcacg
     3301 caaagtcggc tggccgaccg ccaaagaaat cccggcggtg taccgcgacg gcaaacagcc
     3361 ggaggcacaa agtgaagcat aagctcgcgt cttggtggaa aaccatcaag ctgacggcaa
     3421 acttgatggc aggcgtgccc gattatgaaa actacgttgc acagcagcgc aaacataatc
     3481 ccaacgcccc cgtgatgacc aagctgcagt ttcaagacta ttgccgcaaa cgccgctgcg
     3541 gcgcaaacgg cggacgctgc tgttaagcct gcttgaaaca aattccgtct gaacgccgct
     3601 tcagacggaa tttttataat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     3661 cagacagtac aaatagtacg aaaccga
//
LOCUS       DAWIVB010000182         3661 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWIVB010000182 DAWIVB010000000
VERSION     DAWIVB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.182"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..127)
                     /gene="arfA"
                     /locus_tag="WIX60_001839"
     CDS             complement(<1..127)
                     /gene="arfA"
                     /locus_tag="WIX60_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HEZ5306061.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNR"
     gene            209..709
                     /gene="hscB"
                     /locus_tag="WIX60_001840"
     CDS             209..709
                     /gene="hscB"
                     /locus_tag="WIX60_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ5306062.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            807..3557
                     /gene="gyrA"
                     /locus_tag="WIX60_001841"
     CDS             807..3557
                     /gene="gyrA"
                     /locus_tag="WIX60_001841"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ5306063.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
BASE COUNT          990 a         1075 c          875 g          721 t
ORIGIN      
        1 gcctgttgta gctgcctttg cctttccttt tccgttccac cttgtgccgg aacaaatcgg
       61 atttcactaa ggcttttaaa gcattgtcgc gtattttgcc tttattgtgc tgcactttgc
      121 cgcccatatt cagtcctttc gtttaagaag cggcagatta taaggcaaaa acagttttct
      181 gccaaaatct tacatttatc atcctactat gtcccaatat ttcaccctct tccggattga
      241 acccgctttc gatatcgaca ccgaaaactt ggaacaaacc taccgcgcct tggccgcccg
      301 tttccatccc gataaattcg cttcagcttc cgcctttgag caaaagcagg cagtgatgat
      361 gtcttccacc atcaacgatg cctaccgcac cttgaaaaac cccatcgacc gcgccgccta
      421 cctgctgaaa acatcgggca tcgatgccga cgcgccggag cataccgctt tcgcccccga
      481 attccttatg cagcaaatgg aatggcgcga aacgctgatg gaggcacggg caggcaacga
      541 ccttgaatcc ttgaaaaatc tcgacaacga aatccgcgac gaacaagaaa aactgttctg
      601 cggtctgaaa cagtcgtttg cccgacaaga ttacgacaca gccgcacaac aagtccgcca
      661 aggcaggttt ctcgacaaac tccgcaacga aatttcctcg gcattataat ccgcaccgtg
      721 tttcagacgg cgtaaccgcc gcaccgttcc gcgtcaaaat atgctaaaat aagcaacaat
      781 tttttgccat acgaaacatt gaaaccatga ccgacgcaac catccgccac gaccacaaat
      841 tcgccctcga aaccctgccg gtaagccttg aagacgaaat gcgcaaaagc tatctcgact
      901 acgccatgag cgtcattgtc gggcgcgcgc tgccggacgt tcgcgacggt ctcaagccgg
      961 tacaccgccg cgtactgtac gcgatgcacg agctgaaaaa caactggaat gccgcctaca
     1021 aaaaatcggc gcgcattgtc ggcgacgtca tcggtaaata ccacccccac ggcgataccg
     1081 ccgtatacga caccatcgtc cgtatggcgc aaaatttcgc tatgcgttat gtgctgatag
     1141 acggacaggg caacttcgga tcggtggacg ggcttgccgc cgcagccatg cgctacaccg
     1201 aaatccgcat ggcgaaaatt tcccacgaaa tgctggcaga cattgaggaa gaaaccgtca
     1261 atttcggccc gaactacgac ggtagcgaac acgagccgct tgtactgccg acccgtttcc
     1321 ccacactgct cgtcaacggc tcgtccggca tcgccgtcgg catggcgacc aatatcccgc
     1381 cgcacaacct ttctgatacc gtcaatgcct gcctgcgcct gctcgatgca cccgacaccg
     1441 aaatcgacga actgatcgac attatccaag cccccgactt cccgaccggg gcaaccatct
     1501 acggcttgag cggcgtgcgc gaaggctata aaacaggccg cggccgcgtc gttatgcgcg
     1561 gtaagaccca tatcgaaccc ataggcagaa acggcgaacg cgaagccatc gttatcgacg
     1621 aaatccccta tcaggtcaac aaagccaagc tggtcgagaa aatcggcgat ttggttcggg
     1681 aaaaaacact ggaaggcatt tccgagctcc gcgacgaatc cgacaaatcc ggtatgcgcg
     1741 tcgttatcga gctgaaacgc aacgaaaatg ccgaagtcgt cttaaaccaa ctctacaaac
     1801 tgactccgct gcaagacagt ttcggcatca atatggtggt tttggtcgac ggacaaccgc
     1861 gcctgttgaa cctgaaacag attctctccg aattcctgcg ccaccgccgc gaagtcgtta
     1921 cccgacgtac gcttttccgg ctgaagaagg cacgccatga agggcatatt gccgaaggca
     1981 aagccgtcgc actgtccaat atcgatgaaa tcatcaagct catcaaagaa tcgcccaacg
     2041 cagccgaggc caaagacaaa ctgcttgcgc gcccttggcg cagcagcctc gttgaagaaa
     2101 tgctgacgcg ttccggtctg gatttggaaa tgatgcgtcc ggaaggattg gctgcaaaca
     2161 tcggcttgaa agagcaaggt tattacctga gcgagattca ggcagatgct attttacgca
     2221 tgagcctgcg aaacctgacc ggcctcgatc aagaagaaat tgtcgaaagc tacaaaaacc
     2281 tgatgggtaa aatcatcgac tttgtggata tcctctccaa acccgaacgc attacccaaa
     2341 tcatccgcga cgaactggaa gaaatcaaaa ccaactatgg cgacgaacgc cgcagcgaaa
     2401 tcaacccgtt cggcggcgac attgccgatg aagacctgat tccgcaacgc gaaatggtcg
     2461 ttaccctgac acatggcggc tatatcaaaa cccagccgac caccgactat caggcgcagc
     2521 gtcgcggcgg gcgcggcaaa caggcggctg ccaccaaaga cgaagacttt atcgaaaccc
     2581 tgtttgttgc caacacgcat gattatttga tgtgctttac caatttgggc aagtgtcatt
     2641 ggattaaggt ttacaaactg cccgaaggcg gacgcaacag ccgcggccgt ccgattaaca
     2701 acgtcatcca gttggaagaa ggcgaaaaag tcagcgcgat tctggcagta cgcgagttcc
     2761 ccgaagacca atacgtcttc ttcgccaccg cgcagggaat ggtgaaaaaa gtccaacttt
     2821 ccgcctttaa aaacgtccgc gcccaaggca ttaaagccat cgcgctcaaa gaaggcgact
     2881 acctcgtcgg cgctgcgcaa acaggcggtg cggacgacat catgctgttc tccaacttag
     2941 gtaaagccat ccgcttcaac gaatactggg aaaaatccgg caacgacgaa gcggaagatg
     3001 ccgacatcga aaccgaaatt tcagacggca tcgaagatga aaccgccgac agcgaaaacg
     3061 cactgccgag cggcaaacac ggtgttcgcc cgtccggtcg cggcagcggc ggtttgcgcg
     3121 gtatgcgcct gcctgccgac ggcaaaatcg tcagcctgat taccttcgcc cctgaaaccg
     3181 aagaaagcgg tttgcaagtt ttaaccgcca ccgccaacgg atacggaaaa cgcaccccga
     3241 ttgccgatta cagccgcaaa aacaaaggcg ggcaaggcaa tattgccatt aacactggcg
     3301 agcgaaacgg cgatttggtc gccgcaacct tggtcggcga aaccgacgat ttgatgctga
     3361 ttaccagcgg cggcgtactt atccgcacca aagtcgaaca aatccgcgaa accggccgcg
     3421 ccgcagcagg cgtgaaactg attaacttgg acgaaggcga aaccttggta tcgctggaac
     3481 gtgttgccga agacgaatcc gaactctccg acgcttctgt aatttccaat gtaaccgaac
     3541 cggaagtcga gaactgaaaa tcatctcccg atgccgtctg aagattcaga cggcatttat
     3601 tttatccctc atccgtcatc cagcttctca caatatagcg gattatagtc aattaaaaac
     3661 a
//
LOCUS       DAWIVB010000183         3556 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWIVB010000183 DAWIVB010000000
VERSION     DAWIVB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.183"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            62..333
                     /locus_tag="WIX60_001842"
                     /pseudo
     CDS             62..333
                     /locus_tag="WIX60_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            373..1407
                     /locus_tag="WIX60_001843"
     CDS             373..1407
                     /locus_tag="WIX60_001843"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ5306064.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1613..2194
                     /locus_tag="WIX60_001844"
     CDS             1613..2194
                     /locus_tag="WIX60_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ5306065.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIAQHHL
                     PEFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2263..2445
                     /locus_tag="WIX60_001845"
     CDS             2263..2445
                     /locus_tag="WIX60_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ5306066.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            2442..3203
                     /gene="kdsB"
                     /locus_tag="WIX60_001846"
     CDS             2442..3203
                     /gene="kdsB"
                     /locus_tag="WIX60_001846"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ5306067.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEASVALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAIRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            3226..>3556
                     /locus_tag="WIX60_001847"
     CDS             3226..>3556
                     /locus_tag="WIX60_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ5306068.1"
                     /translation="MQQHIEKWQHLSREEQKILAEVWGLVQNDDQEVHYEMLKLNAPD
                     EASGEFWFRMAETLSTLPPNRSLGLRMNGGRLATAVSILSVMIEDNPDIPQLWAQKIT
                     ALNYSGLN"
BASE COUNT          890 a          878 c          984 g          804 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataaatg aaagatactg aaaaatgaaa gatactgaaa
       61 aatgaaagag atgaaacctg tccgttatca tattggcgat atgcccgaga cttcaaaaca
      121 aaccgcctcc cgtcatgacg acagggcagt gggtgttgac gatgattgtt ttcatgattc
      181 ctttggtcaa tttttgtttg ggtgttcggc agaggcaacc cgaaccgcgc caatttctgt
      241 aaagcgcagt tgcttattta cctgattggt tcgcttatcg gtttggtctt cgcgttgttt
      301 ataggtgggt ctgtatcagg tacgcatgat taatgccccg ggctgatttt gcttcgagga
      361 tttgtatcga atatgccgaa ttgtttcaaa tttcataccg ttatcgaacg gcattggcaa
      421 aaaccttatc cggttttgtc ttttctgctt aagccgctct ccgggctgtt tgccaaaatt
      481 gcggcaaaac ggcggacgga ttttttatcg ggaaaacggc aaagcgaaaa gctgcccgtg
      541 cctgtggtcg tggtcggcaa tattcacgcg ggtgggacgg ggaaaacgcc gattgttgcc
      601 gcgctggtgt cgggtttgca ggaaaagggc gtcaaggtcg gcatcatcag ccggggctac
      661 gggcgcaaga gcaaggcggt tcatgtattg aatgctgaga gccgagcgga agatgcgggc
      721 gatgagcctt tgctgctgtt ccgcaaaacc ggtgcgccga cggcggtggg cagcagccgt
      781 gcagaggcag gcagggcgtt gctggcggcg catcccgaca tcggactgat tgtggcggac
      841 gacggtttgc agcattacgc cctgcggcga gatgtggaaa tcgcggtgtt tccggcggcg
      901 gatacggggc gcacggattt ggatttactg cccaacggca gtttgcgcga acctttgttg
      961 cggctggatt cggtggatgc ggtcgtcgtc agcggcggca aggcggatgc gctgtttagg
     1021 ccgtctgaaa atatgtttca cagccgtatc gaagcgggac ggatttaccg tttgaacaat
     1081 ccgtccgaaa tactggacac aggccgtctg aaaaatcaaa ccgtcgtcgc cgtggcaggt
     1141 attgccaagc cggcgcggtt ttttgattcg ttgcggaata tgggcattac cgtgaagcga
     1201 accgtcgcgc tgcccgacca cgccgacatt tcggcggcag atttgcccga tgcggacgcg
     1261 gtcattatta cggagaaaga tgcggtcaaa ttttcagacg gcatttgcac cgataatgtt
     1321 tgggtgttgc ccgtttgtgc gataatcgaa cctgatttgg cggcgtttgt gttggagcgg
     1381 ttggaagatg taccgaaggc cgtctgaaag cacggtttgg gcggagtgat tacggatttg
     1441 aataagaacg cctcgcgcca tcattcccgc gcaggcggga atctaagtct cgaattttca
     1501 ggaatgccta ggaggctcca gaaatcccaa atctccggat ttccacttgg acaggaatga
     1561 gaaaaccggt cgtatttttt atctgcatta atcattcatt aaaggattga atattaaact
     1621 gaaaaccttg ttattgccct tcgccacgct ggcattgtgc accaatgctt ttgccgcccc
     1681 gcccagcgac gcgtcgttgg cgcgttggct ggatacgcag aattttgacc gggatataga
     1741 aaaaaatatg attgagggct ttaatgccgg atttaaaccg tatgcggaca aagcccttgc
     1801 cgaaatgccg gaagcgaaaa aagatcaggc ggcagaagcc tttaaccgtt atcgtgagaa
     1861 tgttttgaaa gatttgatta cgcccgaagt gaaacaggct gtccgcaata ctttattgaa
     1921 gaatgcccgt gagatataca cgcaagaaga aattgacggc atgattgcct tttacggttc
     1981 gcctgtcggt cagtccgtcg ttgccaaaaa tccgcgctta atcaagaaat cgatgagtga
     2041 aatagcggta tcttggactg cattgtcagg gaaaatcgcg caacatcatc tgcccgagtt
     2101 tacggaagag ttgcggcgca tcatctgcgg cggtaaaaat cccgatgcgg gctgtaaaca
     2161 agccggacag gttgggaaaa ggcatcagaa ataaatgata gccgtctgaa atattgaaga
     2221 gggcatccga ttgattgaac catcaaaccc gaaagcaacc ctatggaaaa aaaattctta
     2281 gacatcctcg tctgccccgt taccaaaggc aggctggaat atcatcagga caaacaggaa
     2341 ttgtggagcc gtcaggcgaa gcttgcctat ccgattaaag acggcattcc ctatatgctg
     2401 gaaaacgaag cgcgagcgtt gagcgaagag gaactcaaag catgaccgaa ttcgtcgtat
     2461 tgattccggc gcggctggat tcgtcgcgcc tgcccggaaa agccttggcg gacatccacg
     2521 gcaaaccgat ggtcgtgcgc gttgccgaac aggcggcaaa aagtaaagcc gcgcgcgtcg
     2581 tcgttgccac cgaccatccc gatattcaga cggcctgtca ggcgcacggt atcgaagtcg
     2641 tcatgacttc aaaccggcac gaaagcggca cgacgcgcct tgccgaagcc tctgtcgcgc
     2701 tgaagctgcc gccgcatttg attgttgtga acgtacaggg tgacgagccg ctgattgccc
     2761 ccgaactcat cgaccgcacc gccgaagtac tcgtcgaaaa caacgtccaa atggcgaccg
     2821 ccgcccacga attgcacgat ttcgacgaat tgatgaatcc caacgccgtc aaagtcgtcc
     2881 tcgacaaaaa ccgcaacgcc atctacttca gccgcgcccc gattccctat ccgcgtgatg
     2941 cgatacgtgc cggaaaacgc gaaatgccgt ctgaaaccgc cgtcctgcga catatcggca
     3001 tctacgctta ccgcgccggc ttcctgcaac gctatgccga aatgagcgtt tcgccgctgg
     3061 aaaccatcga atcgctggaa cagctgcgcg tcctgtggca cggttatccc attgccgtcg
     3121 aaaccgccaa agaagccccc gccgccggtg tggatacgca agaggacttg gacagggttc
     3181 gcgccgtatt tcagaccgta taaaacaggt tcaaagggaa aagatatgca gcaacatatt
     3241 gaaaagtggc aacacttgag ccgggaagaa cagaaaatcc ttgctgaagt atggggtctc
     3301 gtgcaaaacg acgatcagga ggttcactat gaaatgctca aattgaacgc acccgatgaa
     3361 gccagcggcg aattttggtt cagaatggca gaaacactca gcaccctgcc gcccaaccgt
     3421 tccctcggcc ttagaatgaa cggcggcagg ctggcgaccg ccgtatccat cctttccgtc
     3481 atgattgaag acaatcccga cataccgcag ctttgggcgc aaaaaattac cgcgctcaat
     3541 tatagtggat taaatt
//
LOCUS       DAWIVB010000184         3551 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWIVB010000184 DAWIVB010000000
VERSION     DAWIVB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.184"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(198..431)
                     /locus_tag="WIX60_001848"
     CDS             complement(198..431)
                     /locus_tag="WIX60_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306069.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFLSFSHFR"
     gene            complement(461..3226)
                     /locus_tag="WIX60_001849"
     CDS             complement(461..3226)
                     /locus_tag="WIX60_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ5306070.1"
                     /translation="MRSSFRLKPICFYLMGVTLYHYSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWER
                     DLQRQQWKYKPYKNYNNQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNLF
                     KLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQK
                     YPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKNR
                     FPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALKK
                     DIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYKKHCNRSCGIYEPVLKK
                     YGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPERA
                     NTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSSTG
                     LAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNAS
                     KEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYID
                     GTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRYI
                     DPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
BASE COUNT          838 a          865 c          871 g          977 t
ORIGIN      
        1 aaaagcagaa accgctccgc cgtcattccc acgaaagtgg gaatctagaa atttaatgtt
       61 gcggcactaa ccaaaaaaac cgaaaccgaa cggtctagat tcccgcctgc gcgggaatga
      121 cgaatccatc cgtacggaaa cctgcaccac gtcattctca cgaaagtggg aatccggaaa
      181 gctaaagcca caggaattta tcggaaatgg ctgaaactta aaaaaactga ttcccgcttt
      241 tgcgggaatg acgactcata aattacccga aacaaccaaa aataaccgaa atcaaaaacc
      301 ggcttccctg cgtgggaatg aaatggattt ttctttgagt tgccgatgcg ataaaacgga
      361 ttggcatagg cttgcggcag gatgatgcgt ttggggaaac aaatgccgtc tgaagggctt
      421 tcagacggca ttgcggtttt ctacaaaatg cgggctgcct ttaaaacttg tagctcatcg
      481 tcatcaaaaa ggtgcgtccg cgtgcaaaat tggtcaatac gcttttgctt gtgccgccgt
      541 atttgccgtt gcacaacgtt ttatcagcat tacacgttac gtcttcgtcc ttgtctttcg
      601 ggtcgaacga gctgtaataa cgctgcgttg ccgcatcatt gcccgcatcg agcggatcga
      661 tataacgcct gtcgaacaga tttttgactt cggcgcggaa aataaggttt ttcttcggct
      721 cgtaagcggc gtaaaaatca aaaatcaaag gctggcgggc aagagtttcg gtttgtttga
      781 tggaacgctt gcccagttgc cggaaattgc tggtatttcc cccgttggtg ccgtcgatat
      841 agcgttcttc agccgtcgcg cggatgctct tgccgaaata gcgcatcgcg ccgcccaaag
      901 tcagtttgtt gcccaaccag cgcgtaccga cttccaaacg tccgtaatct cgcggcaggg
      961 cggaaaccct gctcaaccca taaccttgtt tgagttggtc ttctttggac gcattgttgg
     1021 gcgattcgct cgcatcgctg aagttggtcg gttgcgtgct tttttgatag gcgtaagaaa
     1081 ggttggtgaa aaaacgccca taatcgtaat tcagctccaa ctcaaaaccg tgtttgtgca
     1141 ctttgtcttt gaaattgcga tgttggatgg tgtaggcaag cccggtgctg ctgacccagc
     1201 tcggaatatc cccgttcaaa tcccaccatt tcccgtaaac gttgtggatg tagttgtcga
     1261 tgcggctgcg gtagccgacc agttttaatc ctaatgtatc atcttgtttt aacaatcctt
     1321 ttttataggt attgaagcca aattgccaag tgtttgcgcg ctctggtttt aaggcggtgt
     1381 gaacgccgga gtcgccgatt tgggaaaaat acatttcttg gatgttgggc atacggtgtg
     1441 tgcgcgaata gctggcgaac ggcatgaaat aatcgccgaa gtccgcacta atgctgaccg
     1501 aatggttgtt ggcgcgcttt ttgccgtatt ttttcaatac gggttcataa atcccgcagc
     1561 tccggttgca atgtttcttg tatgtcggcg agttttctcc gaatgcccgc ttaaattcgt
     1621 catccgagcc gtaatagccc gtatattcgc cgccgaaacg gtagccgacg gtattggtgc
     1681 tgtagtttaa gcggtaaatg tcttttttga gcgcggcatc gaagtagaac gtgttgaaat
     1741 attggctgcc ggccggttgg acaatggttg atttttgggg cagcagccct ttatcgccct
     1801 taaaccgccc caaataggaa taaagcccgt tgtcctgatc aggaccgtcg aaaaacagcc
     1861 ccaattcttc aggaaagcgg tttttgccgt attcgttgtg gaaataattg aagcccaaag
     1921 tggtttgcaa ctcggtttcg cggggcagcc ggaaggtggc ggtgttgttg aggtcgagga
     1981 ttttcgcgtt gttgtaggtt tcaaaatcct ttaaaagccc ccagcctgta aacttcgacc
     2041 ctttcggata tttctgcctg cccgaattgt aggctgcggt cagattgagg ttggtatacg
     2101 ggttcaaaga caaaccgtaa ttgaactgat aattgcggtt gatgattttg cggctgccga
     2161 ttttggtgtt taaatcgcga aattgcgccg tgtatttatt gaatacgccg tcgtattcca
     2221 atttaaacag attgcctgcc gactgctgct tcaggctgga cggatcgatg ggggtaatgt
     2281 cgtattgcgg tgccaggttt tcccgccagc ttttgtcatg ctcttcgatg tatttttgta
     2341 gttcttggtt gttgtaattt ttatacggct tgtatttcca ctgttgcctt tgtaaatccc
     2401 gctcccattt tccgctgtcg gaattgaatt tcaaagcacc ctcttgtaca aaatatcgct
     2461 gcttgcgccg ttccaaatat tccgcgccaa aatttccgat gtgctgcccg ccgccgccca
     2521 cgcggtaatt ttgcgccacg ctgcgcctgc tgtgcccgta aagcacaccg acagatgctc
     2581 cgctttccag ccatttgcgc gcacctatcg ccgccatcgc attacctttg gttgaattgg
     2641 tgccggtcag accttttagc agcaggccgt aggtattatt gccctgaacg acgtcatcca
     2701 cgcctaaagt ccgcagattc gccgaaccgg caaggctgtt gatgcctgcc gagccgctga
     2761 agctgccttt gacgacatcc agtccggcaa taaaattgct gtcgacagat gcaccgaatt
     2821 gagatgaacc gcctgccctg cccgcatcgg tagaagtcga ataaaaggtc tgcgtgatgc
     2881 cgtccaccat cgtattgacc cgcccgaacc cgctgtcgcc gcgaatattc aaagacacaa
     2941 tgcccgagct tttatcttgc tgtgtaaacg caccggggat gctgcgtacg atgttgtcga
     3001 ggttttcgct ggatttgaat atatcctgac gggtcgatac ggcacgcgca tcggtaaaca
     3061 cttttttgtc tttcggtacg cgcttcgcct tgacgtgcac atcttccaaa acctgtatct
     3121 gcgcctcgct gcccgcgcgc cctgcatctt cggcataact ataatgatat agcgtaacac
     3181 ccataaggta aaaacaaatc ggcttcaacc ggaaagaaga tctcatattt tcctcaacaa
     3241 taaacagtca gacaattagg aaatatacta gcattttttt caggcggcat aaacattaaa
     3301 aaagtgtaaa tttgatatac cgtctgaaga tttcaattgg atatttatag tggattaaca
     3361 aaaaccagta cagcgttgtc tcgctttagc tcaaagagaa cgattctcta aggtgctgaa
     3421 gcaccaagtg aatcggttcc gtactgtttg tactgtctgt ggctcgccgc cttgtcctga
     3481 tttttattaa tccgctataa agaccgtcgg gcatctgcag ccgtcattcc cgcgcaggcg
     3541 ggaatctagt c
//
LOCUS       DAWIVB010000185         3547 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWIVB010000185 DAWIVB010000000
VERSION     DAWIVB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.185"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            1..1775
                     /locus_tag="WIX60_001850"
                     /pseudo
     CDS             1..1775
                     /locus_tag="WIX60_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            1899..3449
                     /locus_tag="WIX60_001851"
     CDS             1899..3449
                     /locus_tag="WIX60_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ5306071.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPASLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNGYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFINSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT          995 a          875 c          905 g          772 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aggcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gcgaaagcaa cacgctgacc ggattgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttgaca ggtatgtacg gcacgcaagg cacgaccagc
      241 gtgcaagtcg gctccgcaca actgattcaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgatggcg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc tgatgaaggc
      361 aaccgcaaaa tcggtttggc ccggttcggc aacaaccgcg cccaaggtac gttcgacttg
      421 ggccaacgtt tcggcgaaaa caaagcattc ggcgtgcgcg ccaacggcaa actgcgccac
      481 ggcgacaccc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtc ctgactttgg
      661 gcggacgttt ccaagcggtt gaatacacca ataagaaaaa atcacgcgga acggcagccg
      721 gagctagcaa ggtagaagtg aaggcaaacg gaaatattga gcatcaattg gcatcgttta
      781 cagccgacta tcccaattct tgggagaact ctgccaactt ggatgccaat atcaaagtca
      841 acagcctggc tttgtccgac accttgggct tcgtggataa caaataccgc ctgactttgg
      901 gcggacgttt ccaaacagtc gaatataccg acaaaaaatc acagagcggc gatgccaaac
      961 gtttcagccc gatgctgatg gcggcgtggg tgccgcaacc tgatttggtt gtgtacggca
     1021 actatatgga agacttggag cctgccgaca tcaaaaccga tgattccggc gaaacgacaa
     1081 tggcgaaacc gcgtgtcagc cgccagtttg aagtgggcgt gcgtaaaaac tggggcaatt
     1141 tcgtaaccac gttaaacgcg ttccaaatca aacgcccggg ctactggcgc ggtcatacaa
     1201 ctaccaagac aacaaaaggg gtaacaacaa cgctgaccta tggcaataat tctgatttcg
     1261 cccgctataa ggcgcaaggc ggcgcggccg gcgacgagca aggtatggaa cgcaatcgcg
     1321 gtattgagtt caacacctac gcgaacttgc tcaacaatac tttgcgtccg actttgggtc
     1381 tgatgtatct gcaatcgacc gtgaaagagt atccaaattc acgcgatatg ctggttaacg
     1441 gcgtacaagt cgccaatccg cgcgtaattg ccaaagcagg tttggaatgg gatacaccgt
     1501 ttgccaaagg cttgacttta aacggtaatg tttcgtattt cggcaagtct gaccaagaca
     1561 cgcaaaaaca atacgccttc ccgtcctata ccttgattga cgtaggcgcg cgctacaaaa
     1621 ccaagcttgg caaaaacact ctgaccgtca gcagctcggt ggaaaacctg ttcaacaaaa
     1681 actattggca ggtacagcgc ggccaatacg accgcagctt cgccgtcgtc ggtatgccgc
     1741 gcacttactg gctgaaggcg gaattggatt tctgatttcc ctatatccgg aaaatgccgt
     1801 ccgaacaggt ttcagacggc atttctttta aagagatatg gcgggatacg cttaaataat
     1861 gctattctag cgtctttgaa acctgcttgt ttgaagtgat gaaaaaacct ttgatttcgg
     1921 ttgcggcagc attgctcggc gttgctttgg gcacgcctta ttatttgggt gtcaaagccg
     1981 aagaaagctt gacgcagcag caaaaaatat tgcaggaaac gggcttcttg accgtcgaat
     2041 cgcaccaata tgagcgcggc tggtttacct ctatggaaac gacggtcatc cgtctgaaac
     2101 ccgagttgct gaataatgcc cgaaaatacc tgccggataa cctgaaaaca gtgttggaac
     2161 agccggttac gctggttaac catatcacgc acggcccttt cgccggcgga ttcggcacgc
     2221 aggcgtacat tgaaaccgag ttcaaatacg cgcctgaaac ggaaaaagtt ctggaacgct
     2281 tttttggaaa acaagtcccg gcttcccttg ccaataccgt ttattttaac ggcagcggta
     2341 aaatggaagt cagtgttccc gccttcgatt atgaagagct gtcgggcatc aggctgcact
     2401 gggaaggcct gacgggagaa acggtttatc aaaaaggttt caaaagctac cggaacggct
     2461 atgatgcccc cttgtttaaa atcaagctgg cagacaaagg cgatgccgcg tttgaaaaag
     2521 tgcatttcga ttcggaaact tcagacggca tcaatccgct tgctttgggc agcagcaatc
     2581 tgaccttgga aaaattctcc ctagaatgga aagagggtgt cgattacaac gtcaagttaa
     2641 acgaactggt caatcttgtt accgatttgc agattggcgc gtttatcaat cccaacggca
     2701 gcatcgcacc ttccaaaatc gaagtcggca aactggcttt ttcaaccaag accggggaat
     2761 caggcgcgtt tatcaacagt gaagggcagt tccgtttcga tacactggtg tacggcgatg
     2821 aaaaatacgg cccgctggac atccatatcg ctgccgaaca cctcgatgct tctgccttaa
     2881 ccgtattgaa acgcaagttt gcacaaattt ccgccaaaaa aatgaccgag gaacaaatcc
     2941 gcaatgattt gattgccgcc gtcaaaggag aggcttccgg actgttcacc aacaatcccg
     3001 tattggacat taaaactttc cgattcacgc tgccatcggg aaaaatcgat gtgggcggaa
     3061 aaatcatgtt taaagacatg aagaaggaag atttgaatca attgggtttg atgctgaaga
     3121 aaaccgaagc cgacatcaga atgagtattc cccaaaaaat gctggaagac ttggcggtca
     3181 gtcaagcagg caatattttc agcgtcaatg ccgaagatga ggcggaaggc agggcaagtc
     3241 ttgacgacat caacgagacc ttgcgcctga tggtggacag tacggttcag agtatggcaa
     3301 gggaaaaata tctgactttg aacggcgacc agattgatac tgccatttct ctgaaaaaca
     3361 atcagttgaa attgaacggt aaaacgttgc aaaacgaacc ggagccggat tttgatgaag
     3421 gcggtatggt ttcagagccg cagcagtaat gggtcaaatg ccgtctgaaa tgttcaggcg
     3481 gcattttgtt gtattggaaa ctgccgtttt caaccattcc tttagataag ccgtgtcgga
     3541 ttcgttt
//
LOCUS       DAWIVB010000186         3544 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWIVB010000186 DAWIVB010000000
VERSION     DAWIVB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.186"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            100..1215
                     /gene="lst"
                     /locus_tag="WIX60_001852"
     CDS             100..1215
                     /gene="lst"
                     /locus_tag="WIX60_001852"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ5306072.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKEKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKTGDETGGTVRILLGSPDKEMKEISEKAAKNFKIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKN"
     gene            complement(1380..1838)
                     /locus_tag="WIX60_001853"
     CDS             complement(1380..1838)
                     /locus_tag="WIX60_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ5306073.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            1957..2778
                     /locus_tag="WIX60_001854"
     CDS             1957..2778
                     /locus_tag="WIX60_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ5306074.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2829..3275)
                     /gene="yciA"
                     /locus_tag="WIX60_001855"
     CDS             complement(2829..3275)
                     /gene="yciA"
                     /locus_tag="WIX60_001855"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ5306075.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
BASE COUNT          835 a          767 c         1023 g          919 t
ORIGIN      
        1 tattcggtaa ctgtcggaat atctgctaaa attccgcatt tttccgtccc gggacactcg
       61 gggcgtatgt tcaatttgtc ggaatggagt tttagggata tgggcttgaa aaaggcttgt
      121 ttgaccgtgt tgtgtttgat tgttttttgt ttcgggatat tttatacatt tgaccgggta
      181 aatcaggggg aaaggaatgc ggtttccctg ctgaaggaga aacttttcaa tgaagagggg
      241 gaaccggtca atctgatttt ctgttatacc atattgcaga tgaaggtggc ggaaaggatt
      301 atggcgcagc atccgggcga gcggttttat gtggtgctga tgtctgaaaa caggaatgaa
      361 aaatacgatt attatttcaa tcagataaag gataaggcgg agcgggcgta ctttttccac
      421 ctgccctacg gtttgaacaa atcgtttaat ttcattccga cgatggcgga gctgaaggta
      481 aagtcgatgc tgctgccgaa agtcaagcgg atttatttgg caagtttgga aaaagtcagc
      541 attgccgcct ttttgagcac ttacccggat gcggaaatca aaacctttga cgacgggaca
      601 ggcaatttaa ttcaaagcag cagctatttg ggcgatgagt tttctgtaaa cgggacgatc
      661 aagcggaatt ttgcccggat gatgatcgga gattggagca tcgccaaaac ccgcaatgct
      721 tccgacgagc attacacgat attcaagggt ttgaaaaaca ttatggacga cggccgccgc
      781 aagatgactt acctgccgct gttcgatgcg tccgaactga agacggggga cgaaacgggc
      841 ggcacggtgc ggatactttt gggttcgccc gacaaagaga tgaaggaaat ttcggaaaag
      901 gcggcaaaaa acttcaaaat acaatatgtc gcgccgcatc cccgccaaac ctacgggctt
      961 tccggcgtaa ccacattaaa ttcgccctat gtcatcgaag actatatttt gcgcgagatt
     1021 aagaaaaacc cgcatacgag gtatgaaatt tatacctttt tcagcggcgc ggcgttgacg
     1081 atgaaggatt ttcccaatgt gcacgtttac gcattgaaac cggcttccct tccggaagat
     1141 tattggctca agccggtgta tgccctgttt acccaatccg gcatcccgat tttgacattt
     1201 gacgataaaa attaatcgca tagcaaatca aaatagaaaa tggcggagtg cgtggggtaa
     1261 aaataaggat agcgtttttt catttggatt gacgataatt tctgattgct ttgcgtgtgc
     1321 tgaaatggca aagaaaatgc cgtctgaagt cttcagacgg cattgttttg ttttggatgt
     1381 tattcgggcg cgcggaaact gtcgtggcag gatttgcagc ttgcgccggt ttcgccgtag
     1441 gcggctttga tttcgtccag tttgccggtt tgggcggcgg cgttgagttt ttcgacggcg
     1501 gcggcgaatt ttgttttttc ggcttcaaat tttgcaccat ccgaccaaac ggcgggcagt
     1561 gcgcggccgt tgccttgcgg atcggactca aaaagtgtga acggtttctt gctgctttcg
     1621 gcaaacgacg ctgccgcctg tttgaatttt tcgacatcgt aaggttcttc gtctttgacc
     1681 attttgccca tgcgtgtgaa ttcgggcatc atggatttga acgcggcggt gcggttttcg
     1741 gaaatttcgc ctttgggttg ggaaggtatt ccgctgcctc cgcaggcgga aaggagcagt
     1801 gtgatggcgg cagcagcaag gctgatttgg gttttcatat tgaatgtgtc ctgtcgtggt
     1861 ggtatggttg ttgtcatttt cagtcggcgc gatgccgtct gaagggtgtt atgatacctg
     1921 aaacaggttt gggaaacgag aaagaaagga aaaacaatgg ctgttttaat taccggtgct
     1981 tcggcaggat tcggcgaagc gatgtgccgt gcgtttgtcg gggcgggata ccgcgttatc
     2041 ggtgcggcgc gccgtgcgga caggcttcag gccttggcgg atgaattggg tgctttgttt
     2101 taccctttgg aaatggacgt gtcgcgacgc gagtcggtgg aaaacgcctt aaacggcatc
     2161 cccgatgaat tttccgacat cgactgcctc atcaacaatg ccgggctggc tttgggtctg
     2221 gacacggcgg acaaggcgga ttttgaagat tgggaaacga tgattcaaac caatgttttg
     2281 ggtttgacgt tcctgacgcg caaaattttg ccgcaaatgg tggaacgcgg cggcggttat
     2341 gtgatgaatt tgggttcgat tgcaggcaat tatgcttatg ccggcagcaa cgtttacggg
     2401 gcgaccaagg cgtttgtgcg ccagttcagc ctgaatttgc gcgcggagtt ggcggataag
     2461 aacatccgcg ttaccaatat cgagccgggt ttgtgcggca atacggagtt ttccaatgtg
     2521 cgcttcaaag gcgatgacga gagggcggcg ggcgtgtatg agggtgtgga atttatccgc
     2581 cccgaagata ttgcggaaac cgcattgtgg ctgtaccagc ggccggcgca tatgaatgtg
     2641 aacacgattg aaattatgcc cgtggcgcag acttttgcag gaatgaaagt gataaaaaaa
     2701 gccgtgcccg aagtgcggga agactttgaa aaacagagta tgtcgctgtt ttcccgcatc
     2761 aggtcctggt tcaaatgatg caaaatgccg tctgaagaca gtttcagacg gcatttttac
     2821 gggtattttt acggagtagg caataagccc gccaatttgg ggttgccttc tttcggaatc
     2881 gggcgcggat tgccttccgc atcgatggca acataagtga acacggcttc ggttacgagg
     2941 tagcggtctt cggtaacgca atcgttcatc aaagttttca cccagacgtc gactttaagc
     3001 tggagggaag tgttgcccac gcggacgcaa tgcccgtagc agcagacgac gttgccgacc
     3061 ttgaccgggc ggatgaagtt catttcctga acggcgacgg taacgatgcg tccccgcgcg
     3121 atttccgccg ccaatatgcc gccgcccaaa tccatctgcg acataatcca gccgccgaaa
     3181 atgtcttggt tgggattggt atcgcgcggc atagcgacgg tacgcaggag cagttcgcct
     3241 tgagggcgtt ggcggttgcc ttcttcgtgc tgcataaagt ttccttgttt tattgaaata
     3301 taaatcgaac ctgcacccct gcccgaaacg ggaagtcggg aagtgtgtag tttaacccat
     3361 ttgagacata caagggcggg cgcggaacag attcgcaagg cgtattgtag ggcggggctg
     3421 tagagtgggc ttcagtccgc caatcccgcc aaatcctacc ctaagcaact gaaccgtcat
     3481 tcccacgaaa gtgggaatct agaacgcggg gtttcagtca tttccgatag attcccgccg
     3541 cgtc
//
LOCUS       DAWIVB010000187         3473 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWIVB010000187 DAWIVB010000000
VERSION     DAWIVB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.187"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(493..599)
                     /locus_tag="WIX60_001856"
                     /pseudo
     CDS             complement(493..599)
                     /locus_tag="WIX60_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1018..1788)
                     /gene="xth"
                     /locus_tag="WIX60_001857"
     CDS             complement(1018..1788)
                     /gene="xth"
                     /locus_tag="WIX60_001857"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ5306076.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPALPDDPQRRVIAATVSGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1848..2129)
                     /locus_tag="WIX60_001858"
     CDS             complement(1848..2129)
                     /locus_tag="WIX60_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ5306077.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRVA"
     gene            complement(2221..2508)
                     /locus_tag="WIX60_001859"
     CDS             complement(2221..2508)
                     /locus_tag="WIX60_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306078.1"
                     /translation="MSGKEPLNPNPVIASGRKCRLKDSSDGIFRKGRDALEKTAFQAG
                     ILFARQRGGIGRAGKRTGGGSFMPSESPPLRLFAKKVGKGTYNPVQSSINI"
     gene            complement(2536..3417)
                     /gene="nadC"
                     /locus_tag="WIX60_001860"
     CDS             complement(2536..3417)
                     /gene="nadC"
                     /locus_tag="WIX60_001860"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ5306079.1"
                     /translation="MPSEKTLFPLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
BASE COUNT          848 a          825 c          874 g          926 t
ORIGIN      
        1 ctgatgcaga tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
       61 caaatagtac ggcaacgctg tactggttta aatttaatcc actatatttt gtaatagctg
      121 ccattatgcc tactaggata tataccttct atataatcct tatttaaata aaaaccctta
      181 taaaaatctg tgttatctgt cgggattgaa aaaaagattt tatctttctc atcaggttta
      241 tatttgctat caatatcaaa aagagaataa ggattacaat gagttcgaca tactaggtaa
      301 aaatttatat cttggatcat gaaatattcg atatttatca ggatcgaaag atgctaaaaa
      361 ttcatcaata ttggtactgg atattttgtc atcactaata aacatcgcta gagatgggcg
      421 actttgattt tccatttgtt gattagtaaa ggatataaat actaacttgc cattgtcaaa
      481 ccacatagat ttatatagtg gattaaattt aaaccagtac gtcgttgcct cgccttgccg
      541 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatag
      601 ttattgcctt tgggagaatc aattctgaca gcacataaca tagatttatc tttacagtcg
      661 ttatattgag tttcatcaaa ctcaactgca tggcacaata aaggcaatag gaaaaacagg
      721 gttattaatt tattactata tataagacgg ctttctattt attcgaaaat aaatctagtt
      781 ttcaatattt gattttgttt tggattagtg tacataataa attttactgc tccaaaatct
      841 gatattaacc ctatgtattt cataaatttt tcaggaaaat aggctgtgtt tgaacaatcc
      901 gccgatttcg gctgtttgcc ggggaaaggc aattattttt cagtcagata gaaagctatc
      961 ctattgaata aattgaagat aaggatgctt gtcaacgcca tatcaaaaca cggtctttta
     1021 ccaatcgaat tctgccgtca ccggcgcgtg gtcgctcgga cgctccagcg cgcgcgtctc
     1081 caaatcgacg cggacatcct tcaacgccgc cgccatcgca ggcgacacca aaatatggtc
     1141 gatacgcagg cccagtttgc gttggaacat cgcgccgcga tagtcgaacc aggtatagaa
     1201 cgcgccttcg ggatggactt ggcgcaggct gtcggtcagt cccaaatcca gcaggttttg
     1261 aaaccactgc cgttcgacgg acgaacagtg gattttttcg tgccattttt cagggtcgta
     1321 acagtccgca tcggcaggcg cgatattgaa atcgcccagc aacaccagtt tgccgtggcg
     1381 ggtcatttca tcgcggacaa actccgtcag tgcggcaaac cactgttcct tatatttgaa
     1441 tttggggctg tccaaagcct cgccgttgac gcaatagaca ttgatgacgc gcacgccgct
     1501 gacggttgcc gcaatcacgc gccgttgcgg atcgtccggc agtgcgggca aaccgaaatg
     1561 cacgtcctgc ggcacgctgc ggctgacgat tgccacgccg ttgtaggttt tctgcccgct
     1621 ccaaacacag tgccagccca tcatttgcaa agcggcggcc ggaaatttgt cctgatcgag
     1681 tttgagttcc tgcaaaacca aaatatcggg cggattgtcg gcaagcaggt tttgcacctg
     1741 cggcagccgc acattgagcg aattgacatt ccaagtggtg attttcataa catttccgaa
     1801 aaatgctgtc tgaaacgttc agacggcaaa aggaaaggat tctaacacta tgccacgcgt
     1861 ttgttttcca aatcgcgaac cgtgtgcaga atcgccgccg cttcttcgga aaccaggcgg
     1921 tattcgataa tgcggtggta acgcgtaaaa tcgactagac cttccacgcg caggcggttc
     1981 aaatggttgg aaactgcggt ggccggcagg gataaggatt ttgccagttc ggcgatattg
     2041 cgttcgctgt ccaacaattg aatcagtatc gccatacgtt cgggatgcgc cataagtttg
     2101 agtatggtgt ggagcggtat tgtgttcatc ggtattccta aaacctttgc cgcccgccaa
     2161 gcggattgac gggcggcaga tatggatgat tgtgattatc tttataaatc gtattataaa
     2221 tcaaatattt atggatgatt gtacaggatt gtatgttcct tttcccactt tttttgcaaa
     2281 caagcgtaaa ggcgggcttt cagacggcat aaaactgccg ccccccgtcc tttttcctgc
     2341 cctaccgatg ccgccgcgct gtcgggcaaa caaaatacct gcctgaaatg ctgttttttc
     2401 taaagcatca cgacctttac gaaaaatgcc gtctgaagaa tctttcagac ggcatttcct
     2461 ccctgaggca ataaccggat tagggttaag cggttccttg cctgacatat cagacagccg
     2521 cccgcaccct aaaactcacg ccacgaaatc caaacctatg tccaacgaac ggctgctgtg
     2581 ggtcagatag ccgagggcga tgccgtccac tccggtttgc gccacgcgct tcaggcggtc
     2641 gaagccgatg ccgcccgatg cttcgcaata gatggtgtgg gggtgggcgg tttgcgtgtg
     2701 gcagcggttt gccgcttctt tcagggtttc gtcgtccatg ttatccagca aaatccgttc
     2761 cgcgcccgct gcgatggctt cgtccagttg tgccaacgta tccacttcga tttccacgca
     2821 ggtcaatgct ccgacagcct gttttgcctg ctgcacggct tgggcgatgc tgccgcaata
     2881 ggcgaggtgg ttgtctttga tgagcacggc gtcgtccaaa cccatgcggt ggttcacacc
     2941 gccgcctgcc ctgacggcgt atttttgcag gacacgcagc agggggatgg ttttgcggct
     3001 gcacacgatg tctgtaccgt attcggcgac ttcggcaacg gcacgcgcgg tggcggtggc
     3061 gatgccgctt aagtgcgtga ggtagttgag cgcggtgcgt tcggcggcga gcagcgcgcg
     3121 ggcgttgcct tcgacggcgg caagcgtctg acctgcgcgg acggcttgcc cgtctcggat
     3181 ttcggcttgg aagcggacgg acggatccat cgtctgaaag gcgagacgcg ccaagtccat
     3241 gccggcgata acgccgtctt cgcggctgac aaggaagagt ttggcggttt tgtcgggggc
     3301 gatgacggcg gcggacgtaa tatcgccgcg cctgcccaag tcttcgctca aggcttgttc
     3361 tactatgggg cgcaacaggg tgtcgggcag gggaaagagg gttttttcag acggcatggg
     3421 gtttcctttg ttgaaaaaga ttgatgttgc accgatgggg caaaacttca ttt
//
LOCUS       DAWIVB010000188         3466 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWIVB010000188 DAWIVB010000000
VERSION     DAWIVB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.188"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            185..1060
                     /locus_tag="WIX60_001861"
     CDS             185..1060
                     /locus_tag="WIX60_001861"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ5306080.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEN
                     GQAKVRAALKASGQL"
     gene            1069..2265
                     /gene="bamC"
                     /locus_tag="WIX60_001862"
     CDS             1069..2265
                     /gene="bamC"
                     /locus_tag="WIX60_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ5306081.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDKVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2322..3254)
                     /gene="pip"
                     /locus_tag="WIX60_001863"
     CDS             complement(2322..3254)
                     /gene="pip"
                     /locus_tag="WIX60_001863"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ5306082.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT          925 a         1146 c          768 g          627 t
ORIGIN      
        1 cgacggcacc ggcataaacc ggcgaatata ataaaatgat ttcaaacagg aaaaatcaat
       61 ttccaccata cctttcggcg caaacgcccc acatctgcaa cccgccggat ttcacacctg
      121 cggtaaagta ggctacaatc ggaagcatta ccgcccctac accacaactg ataaaaggtt
      181 ctgtatgtta caaggtagct tggttgccct gattaccccg atgaatcaag acggcagcat
      241 ccattacgaa caactccgcg acttaatcga ctggcacatt gaaaacggca cggacggcat
      301 cgttgccgtc ggcacgacag gcgaatccgc caccctctcc gtcgaagaac acaccgccgt
      361 catcgaagcc gtcgtcaaac acgttgccaa acgcgtcccc gtcatcgccg gcacaggggc
      421 aaacaacacc gtcgaagcca tcgccctttc ccaagccgcc gaaaaagccg gggcggacta
      481 caccctctcc gtcgtcccct attacaacaa gccctcccaa gaaggcattt accaacattt
      541 caaaaccatc gccgaagcca cttcgattcc gatgattatc tacaacgtgc ccggccgtac
      601 cgtcgtcagc atgactaacg acactatcct gcgccttgcc gaaatcccca acatcgtcgg
      661 cgtgaaagaa gccagcggca acatcggcag caacatcgaa ctcatcaacc gcgcgcccga
      721 aggcttcgtc gtcctttccg gcgacgacca caccgccctg ccgttcatgc tctgcggcgg
      781 acacggcgtg attaccgtag ccgccaacgc cgcgcccaag ctctttgccg atatgtgccg
      841 cgccgcgctt caaggcgata tcgcccttgc acgcgaactc aacgaccgac tgattccgat
      901 ttacgacacc atgttctgcg aacccagccc cgccgcaccg aaatgggcgg tatccgccct
      961 gggcagatgc gaaccgcacg tccgcctgcc gctcgttccg ctgacggaaa acggacaagc
     1021 aaaagtccgc gccgccctga aagcctcagg acaactttaa atatagccat gccgtccgaa
     1081 ccgttcggac ggcataacgc aacaaacact ttaatatcca tcacacagga tgacacgatg
     1141 acccatatca aacccgtcat tgccgcgctc gcactcatcg ggcttgccgc ctgctccggc
     1201 agcaaaaccg aacagcccaa gctcgactac caaagccggt cgcaccgcct gatcaaactt
     1261 gaagtcccac ctgatttgaa caaccccgac caaggcaacc tctaccgcct gcctgccggt
     1321 tcgggcgccg tccgcgccag cgatttggaa aaacgccgca cacccgccgt ccaacagcct
     1381 gccgatgccg aagtattgaa aagcgtcaaa ggtgtccgcc tcgagcgcga cggcagccaa
     1441 cgctggctcg ttgtcgacgg caagtctcct gccgaaatct ggccgctcct gaaagccttt
     1501 tggcaggaaa acggcttcga catcaaatcc gaagaacccg ccatcggaca aatggaaacc
     1561 gagtgggcgg aaaaccgcgc caaaatcccc caagacagct tgcgccgcct cttcgacaaa
     1621 gtcggcttgg gcggcatcta ctccaccggc gagcgcgaca aattcatcgt ccgtatcgaa
     1681 cagggcaaaa acggcgtttc cgacatcttc ttcgcccaca aagccatgaa agaagtgtac
     1741 ggcggcaaag acaaagacac gaccgtatgg cagccctccc cgtccgatcc caacctcgaa
     1801 gccgctttcc tgacgcgctt tatgcaatat ttgggcgttg acggacagca ggcggaaaac
     1861 gcatcggcaa aaaaacctac ccttcccgcc gccaacgaaa tggcgcgtat cgaaggcaaa
     1921 agcctgattg tctttggcga ctacggcaga aactggcggc gcaccgtgct cgccctcgac
     1981 cgcatcgggc tgaccgtcgt cggtcaaaac accgaacgcc acgccttcct ggttcaaaaa
     2041 gccccgaacg aaagcaatgc agttaccgaa caaaaacccg gcctgttcaa acgcctgctg
     2101 ggcaaaggca aagcggagaa acctgccgaa cagccggaac tgattgtcta tgcagaacct
     2161 gtcgccaacg gctcgcgcat cgtcctgctc aacaaagacg gcagcgcata tgccggcaaa
     2221 gacgcatccg cattattggg caaactccat tccgaactgc gttaaaccat tcaaaacaaa
     2281 agcagcttcc aaacagaagc tgctttttta tgcggaactt tttacaacaa atggggcaaa
     2341 atatcctcaa ctgcctgaac caacgcatcc accaaaggcg gatcgaacgc acgatgccct
     2401 gcctgaacca ccctcaattc cgcttcggga aaggctttcg acagcgccca cgcactctgc
     2461 atcggcgtac acaaatcata ccgcccctgt acgataatag tcgggatatg ccgtattttg
     2521 ccgatattgt tcaaaatcgc cctatcgccc tgcaaccaac cgccgttgac aaaataatgg
     2581 ttttccaaac gcgcgattgc cagcgaggca taagcatctt catccacttc ctccggctcg
     2641 aaacggatca gatagctttc ccaatccgcc caagccttcg cggcagacag gcacacttct
     2701 tcatcttgat gaaacagcaa tccgtgatac gcctcaatca gccggttccg ccgattttca
     2761 gcaatcggcg cgacaaattt ttgccattgt tccggataaa tccggctcac accgcccgcc
     2821 tcgttcagcc acaccgtttc agacggcctg cacaaaaata tcccgcgcaa caccaatccc
     2881 tttacccgtt caggatgggt ttgggcataa gccagcgaca aagtgctgcc ccacgaaccg
     2941 ccgaacacca gccatttccc gatacccagc atttcacgga ctttttcaat atccgccacc
     3001 aaatcccaag tcgtattgtc ttccgcacaa gcatacgggc gcgaacgtcc gcaaccgcgc
     3061 tggtcgatga tgacgatgcg gaacacatcg ggattgaaaa aaccccgaca ttcaggcgaa
     3121 gcccccgcgc ccggcccgcc atgtaaaaaa ataaccggca caccgtcggg attgcccgat
     3181 tcctcccaat aaatttgatg aatttcagac acctgcaagt atccgctatg aaaaggctgt
     3241 tttatctcat acatcatctt ctcccatatt gcttctgaca atccgaacat tcccacacgc
     3301 aacatcacgc acgagacaca agccgccccg aaaggcaaat gccgaaaaat cgcgatatag
     3361 tgaattaaca aaaatcagga caaggcggcg agccgcagac agtacggata gtacggaacc
     3421 gattcactcg tgcttaagca ccttagagaa tcattctctt tgagct
//
LOCUS       DAWIVB010000189         3411 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWIVB010000189 DAWIVB010000000
VERSION     DAWIVB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.189"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            36..704
                     /locus_tag="WIX60_001864"
     CDS             36..704
                     /locus_tag="WIX60_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ5306083.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPAQVKKDGTVNNKCDSGKKGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYTK
                     DQHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRNDRRTV
                     CQYK"
     gene            734..1348
                     /gene="pilV"
                     /locus_tag="WIX60_001865"
     CDS             734..1348
                     /gene="pilV"
                     /locus_tag="WIX60_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ5306084.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSAVDGDFAVDA
                     VKSKKDLAKAQLDRFGYELKNALPDAAAIYYAICKDSLGVAPTLSAGSTFSSNCDASA
                     NGDTLIKVLWVNDSAGDSDIAHTNLETNGNNIVYTYQARVGGRE"
     gene            1345..2286
                     /locus_tag="WIX60_001866"
     CDS             1345..2286
                     /locus_tag="WIX60_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ5306085.1"
                     /translation="MKRKMLNVPKGNYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLSAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVIPDTTQQNSP
                     FSLKRNGIDKLIPIAESSNINYQNFFQVGSALIFQYGIDDVNASTATTVVSSCAAISK
                     PGKQIPTLEDAKKELKIPDQDKEQNGNIARQRHVVNAYAVGRIADEEGLFRFQLDDKG
                     KWGNPQLLVKKVRHMKVRYIYVSGCPEDDDAGKEETFKYTDKFDSAQNAVTPAGVEVL
                     LSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2265..2858
                     /locus_tag="WIX60_001867"
     CDS             2265..2858
                     /locus_tag="WIX60_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ5306086.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCAAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCPANSTDLCIDKKGMEYKKGTRSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2863..3336
                     /locus_tag="WIX60_001868"
     CDS             2863..3336
                     /locus_tag="WIX60_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ5306087.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIENKLEIFVSGYKMNPKIAKKYSVSVKFVDKEKSRA
                     YRLVGVPKAGTGYTLSVWMNSVGDGYKCRDAASAQAHLETLSSDVGCEAFSNRKK"
BASE COUNT          974 a          674 c          865 g          898 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gtcctatgtg tacacgaaaa caacaaggtt
       61 tcacgctaac agagctgctc atcgtgatgg tcattgcagc cattatggcg atgatagccc
      121 tccccaatat gagccaatgg attgcatccc gccgcattgc cagtcatgcg gagcggattg
      181 ccaacctttt gcgtttctcc aggggcgaag ccgtccggct caatctccct gtctatatct
      241 gtcctgctca agttaaaaaa gacggtacgg tcaacaataa atgtgactcc ggcaagaagg
      301 ggcagggaat gttggctttt ggcgataaga atggcaataa gacatatgac ggcgatacgg
      361 cggatgttct tctccgcagt gtggtattga atgatgatat caatgataag cggattaatt
      421 atgccttcaa ccatatcgct ttcggtcaga ctcagccgac cgctgaccgt gtagtttgga
      481 cattcaatca aaacgggacg tttggttata cgaaagacca gcatcttaca aaacaatcca
      541 gcttttttta ttccgacggt tatatccaaa tcgtgctgac agatgcgaga gcagtttcag
      601 atgccgataa gaaattccgt tcggcggtgg ttttgattaa cagcagcggc agggttgagg
      661 tttgtcctag aaacgatagg cgtaccgtat gccaatataa atgattgttt tagtcggttt
      721 gggaaataaa aaaatgaaga ataatgattg cttccgcctg aaaaaccccc agtctggtat
      781 ggcgctgata gaagtcttgg tcgctatgct cgttctgacc atcggtattt tggcactatt
      841 gtctgttcag ttgcggacag tcgcttccgt cagggaggca gagacgcaaa ccatcgtcag
      901 tcaaatcacg caaaacctga tggagggaat gttgatgaat ccgaccattg attcggacag
      961 caacaagaaa aactataatc tttacatggg aaaccatcat acactatcag ctgtggatgg
     1021 cgattttgcg gttgatgccg tgaaaagcaa gaaggatttg gcaaaagccc agttggacag
     1081 gttcggttat gaattgaaaa atgccttgcc ggatgcggca gccatctatt acgccatctg
     1141 caaggattcg ttgggtgttg cgccgacatt gtccgccggt agtacttttt cttcaaattg
     1201 cgatgctagt gcaaatgggg atactttgat taaagtattg tgggtaaatg attcggcagg
     1261 ggattcggat atcgcccata cgaatcttga gacgaacggc aacaatatcg tatataccta
     1321 tcaggcaagg gtcggaggtc gtgaatgaaa cgtaaaatgc taaacgtacc aaaaggcaat
     1381 tatgatggta tgaagggttt taccattatt gaatttttgg ttgcgggcat gctcagtatg
     1441 attgtcctga tggcggtcgg atcgagttac ttcacatccc ggaaattaaa tgatgcggca
     1501 aacgagcgtc tttccgcgca acaggatttg cggaatgcgg caacattgat tgtccgcgat
     1561 gcgagaatgg cgggcggctt cggttgtttc aatatgtccg agcatcctgc aactgatgtt
     1621 attcccgata cgacgcaaca aaattctcct ttttccttaa aaaggaacgg tatagataaa
     1681 cttattccca tagcggaatc ttcaaatatc aattatcaga attttttcca ggttggtagc
     1741 gcattgattt ttcaatacgg aatcgatgat gttaatgcaa gcaccgcgac taccgtcgtc
     1801 agcagctgtg ccgcaatatc gaaaccgggc aagcaaatcc ctactttaga agatgcaaaa
     1861 aaagaattga agattccgga tcaggataag gagcaaaatg gcaatatagc gcgtcaaagg
     1921 catgtggtca atgcctatgc ggtcggcagg attgccgatg aggaaggttt gttccgcttc
     1981 caattggatg ataagggcaa gtggggtaat cctcagttgc tcgtgaaaaa ggttagacat
     2041 atgaaagtgc ggtatatcta tgtttccggc tgtcctgaag atgacgatgc cggcaaagag
     2101 gaaacattca aatatacgga taaattcgac agcgcccaaa atgctgttac gcccgccggg
     2161 gtggaggttt tattgagtag cggtactgat accaagattg ccgcttcttc agacaatcat
     2221 atttatgctt accgtatcga tgcgacaata cgcgggggaa atgtatgcgc aaacagaaca
     2281 ctttgacggg aatcccgact tctgacggac agagggggtt tgcactgttt atcgtgctga
     2341 tggtgatgat cgtcgtggct tttttggttg taactgccgc gcagtcttac aataccgagc
     2401 agcggatcag tgccaacgaa tcagacagga aattggcttt gtctttggcc gaggcggctt
     2461 tgcgggaagg cgaacttcag gttttggatt tggaatatga tacggacagt aaggttacat
     2521 ttagcgaaaa ctgtggaaaa ggtctgtgtg ccgcagtgaa tgtgcggaca aataatgata
     2581 atgaagaggc ttttgacaat atcgtggtgc aaggcaagcc caccgttgag gcggtgaagc
     2641 gttcttgccc tgcaaattct accgacctgt gcattgacaa gaaagggatg gaatataaga
     2701 aaggcacgag aagcgtcagc aaaatgccac gttatattat cgaatatttg ggcgtgaaga
     2761 acggagaaaa tgtttatcgg gttactgcca aggcttgggg taagaatgcc aataccgtgg
     2821 tcgtccttca atcttatgta agcaataatg atgagtaata aaatggaaca aaaagggttt
     2881 acattgattg agatgatgat agtcgtcgcg atactcggca ttatcagcgt cattgccata
     2941 ccttcttatc aaagttatat tgaaaaaggc tatcagtccc agctttatac ggagatggtc
     3001 ggtatcaaca atatttccaa acagtttatt ttgaaaaatc ccctggacga taatcagacc
     3061 atcgagaaca aactggaaat atttgtctca ggctataaga tgaatccgaa aattgccaaa
     3121 aaatatagtg tttcggtaaa gtttgtcgat aaggaaaaat caagggcata caggttggtc
     3181 ggcgttccga aggcggggac gggttatact ttgtcggtat ggatgaacag cgtgggcgac
     3241 ggatacaaat gccgtgatgc cgcttctgcc caagcccatt tggagacctt gtcctcagat
     3301 gtcggctgtg aagccttctc taatcgtaaa aaataaggtt gttttgccaa taccgtctga
     3361 aaatcaatgt tcagacggta tttttatggg tatagtggat taacaaaaat c
//
LOCUS       DAWIVB010000190         3407 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWIVB010000190 DAWIVB010000000
VERSION     DAWIVB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.190"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     regulatory      65..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            235..1458
                     /gene="cytX"
                     /locus_tag="WIX60_001869"
     CDS             235..1458
                     /gene="cytX"
                     /locus_tag="WIX60_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ5306088.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            1455..2555
                     /locus_tag="WIX60_001870"
     CDS             1455..2555
                     /locus_tag="WIX60_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ5306089.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEGLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAIHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAARLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            2577..3194
                     /gene="thiE"
                     /locus_tag="WIX60_001871"
     CDS             2577..3194
                     /gene="thiE"
                     /locus_tag="WIX60_001871"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ5306090.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCAAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          682 a         1076 c          946 g          703 t
ORIGIN      
        1 aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg ccccctgctt ttgtgtacac
       61 ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt
      121 agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc
      181 cgttcccttt tccgcacttc cccgccccat tttcatgttt tttaaggact tgatatgtcg
      241 ggcaatgcct cctctccttc atcttcctcc gccatcgggc tgatttggtt cggcgcggcg
      301 gtatcgattg ccgaaatcag cacgggtacg ctgcttgcgc ctttgggctg gcagcgcggt
      361 ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat
      421 atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc
      481 ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg
      541 atttacgccg gcgcaacggt cagctccgct ttgggcaaag tgttgtggga cggcgaatct
      601 tttgtctggt gggcattggc aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca
      661 cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga tgctgttggc ggttctgtgg
      721 ctgagtgccg aagtcttttc cacggcaggc agcaccgccg cacaggtttc agacggcatg
      781 agtttcggaa cggcagtcga gctgtccgcc gtgatgccgc tttcctggct gccgcttgcc
      841 gccgactaca cgcgccacgc gcgccgcccg tttgcggcaa ccctgacggc aacgctcgcc
      901 tacacgctga ccggctgctg gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga
      961 gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg gtgcggcagg cattttggcg
     1021 gtcgtcctct ccaccgttac cacaacgttt ctcgatgcct attccgccgg cgcgagtgcg
     1081 aacaacattt ccgcgcgttt tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc
     1141 acggtacttg ccgtcatgct gcccgttacc gaatatgaaa acttcctgct gcttatcggc
     1201 tcggtatttg cgccgatggc ggcggttttg attgccgact ttttcgtctt gaaacggcgt
     1261 gaggagattg aaggctttga ctttgccgga ctggttctgt ggcttgcggg cttcatcctc
     1321 taccgcttcc tgctctcgtc cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg
     1381 tctgccgttg ccattgccac cgtatcggta cgccttttct ttaaaaaaac ccaatcttta
     1441 caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga
     1501 ccgcgttgca gcttgcagaa caaggttatc agattgcact tttcgataaa ggctgccgcc
     1561 ggggcgaaca cgccgccgcc tatgttgccg ccgccatgct cgcgcctgcg gcggaagcgg
     1621 tcgaagccac gcccgaagtg gtcaggctgg gcaggcagag catcccgctt tggcgcggca
     1681 tccgatgccg tctgaacacg cacacgatga tgcaggaaaa cggcagcctg attgtgtggc
     1741 acgggcagga caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtag
     1801 cggatgacga aatcgtccgt tggcgcgccg acgacatcgc cgaacgcgaa ccgcaactcg
     1861 gcggacgttt ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa
     1921 tattgtctgc acttgccgac gctttggacg aactgaacgt cccctgccat tgggaacacg
     1981 aatgcgtccc cgaaggcctg caagcccaat acgactggct gatcgactgc cgcggctacg
     2041 gcgcaaaaac cgcgtggaac caatcccccg agcacaccag caccctgcgc ggcatacgcg
     2101 gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa ccgccccgtg cgtctgctcc
     2161 atccgcgtta tccgctctac atcgccccga aagaaaacca cgtcttcgtc atcggcgcga
     2221 cccaaatcga aagcgaaagc caagcccccg ccagcgtgcg ttcagggttg gaactcttgt
     2281 ccgcactcta tgccatccac cccgccttcg gcgaagccga catcctcgaa atcgccaccg
     2341 gcctgcgccc cacgctcaac caccacaacc ccgaaatccg ttacaaccgc gcccgacgcc
     2401 tgattgaaat caacggcctt ttccgccacg gtttcatgat ctcccccgcc gtaaccgccg
     2461 ccgccgccag attggcagtg gcactgtttg acggaaaaga cgcgcccgaa cgcgatgaag
     2521 aaagcggttt ggcgtatatc cgaagacaag attaaagccg cccgaaagga caccttatga
     2581 ccttcccgcc cctaaaatcc ccgctcaaat tctacgccgt cgtccccacc gccgattggg
     2641 tggggcgcat ggtcaaagca ggtgccgaca cggtgcaact gcgctgcaag gccctgcacg
     2701 gcgatgaatt gaaacgcgaa atcgcccgct gcgccgcagc ctgtcagggc agccgtacgc
     2761 agcttttcat caacgaccac tggcgcgaag caatcgaagc gggcgcgtac ggcgtgcatc
     2821 tcggacaaga agacatggac accgccgacc ttgccgccat cgccgccgcc ggtttgcgct
     2881 tgggtttgag tacgcactcc gttgccgaac tcgaccgcgc cctgtccgta caccctagct
     2941 acatcgccag cggcgcgatt ttcccgacca cgaccaaaca aatgcccacc gccccgcaag
     3001 gcttggacaa actgcgcgaa tacgtcaaac aagcaggcgg cacgcccgtc gtcgccatcg
     3061 gcggtatcga cttgaacaac gcccgagccg tactcgccac cggcgtttcc tcactcgccg
     3121 ccgtccgcgc cgtaaccgaa gcggcaaatc ccgaagcggt ggttaaagcg tttcaggctt
     3181 tgtgggatgg ataaaaccga aagaagaaaa ttcaattgcc gtgtaggcaa aacttagccc
     3241 gttatcgcaa acatacttaa ctttaaatgt ggcatatcat caaattccgt cattcccgcg
     3301 taagcgggaa tccgccttaa aacttgagaa accatcattt gaaaaacagt ttccgaattt
     3361 caaaaatgga ttcccgcccg tgcgggaatg acggcaaccg gtcagtt
//
LOCUS       DAWIVB010000191         3365 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWIVB010000191 DAWIVB010000000
VERSION     DAWIVB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.191"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..158
                     /locus_tag="WIX60_001872"
     CDS             <1..158
                     /locus_tag="WIX60_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306091.1"
                     /translation="LKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKTV
                     YLPGLHP"
     gene            170..679
                     /locus_tag="WIX60_001873"
     CDS             170..679
                     /locus_tag="WIX60_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306092.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRNRNLPATATAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(732..1079)
                     /locus_tag="WIX60_001874"
     CDS             complement(732..1079)
                     /locus_tag="WIX60_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ5306093.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1081..1317)
                     /locus_tag="WIX60_001875"
     CDS             complement(1081..1317)
                     /locus_tag="WIX60_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ5306094.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDAGNEVV"
     gene            complement(1676..2167)
                     /locus_tag="WIX60_001876"
     CDS             complement(1676..2167)
                     /locus_tag="WIX60_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ5306095.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(2419..2589)
                     /locus_tag="WIX60_001877"
     CDS             complement(2419..2589)
                     /locus_tag="WIX60_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306096.1"
                     /translation="MPSEIQTAFLLKGFSSTALHKGGFFMPSESPSDGIGLHGRSPPA
                     FKQERMSRMPDF"
     gene            complement(2605..2973)
                     /locus_tag="WIX60_001878"
     CDS             complement(2605..2973)
                     /locus_tag="WIX60_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ5306097.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
BASE COUNT          849 a          816 c          781 g          919 t
ORIGIN      
        1 cgctgaaatt cgaccagttg atactcgaat ttcctgagcg cggggacggc gcatgggtac
       61 acatcggttt ccgacgcaac agcccgcaac gcaaccagat actgaccgca accaagaaaa
      121 acggcaaaac cgtgtatctg cccgggctgc atccttgagg tcgtctgaaa tggatatttt
      181 attgaaatac tggaagccgg taggtgtatt gctgctaatc gtcctgattt ttaccgcatg
      241 gcatttcgac cgtgccgaaa aataccgcat gggacgggag gctgctgctg ccgaaatctc
      301 gaatcgtctg aaagacggct atatcgagca ggcaaagcag gcgcgttctg ccgagcagaa
      361 ggccgctgcc gcgtttgccg aacgacaaac taaattagaa gaggaaaaac aaaatgctga
      421 aaaaactgtt gccgctatgc gtcttgagct taaccgcctg cgccactacg ccgccgccca
      481 aaatcgcaac agaaacctgc ccgcaaccgc taccgccgcc accgcatctg atggcgcgtc
      541 agattcccaa ggctggctat tattcggaca gtgcgctgaa aaatatgcag gactggcaga
      601 aacagccgac ggacatgcag ccgatttaag ggaatggcag gcatatggtc atgcagtaag
      661 cagtcagcgt gccgaataag caaccgcccg aacctgtaag aaaagattac aggttcgggc
      721 ggtttcagca tttaatcgaa taagacggcg ccgatgcgcg ccagcacatc gtccaataca
      781 taatcgggta cagtttcttt aaaagaagcc ttgcggattt gccagtctag agatttgagc
      841 tggtggcagt ggacattgcc ctgcgtttcc gttcctgcac cgagtaaggt tgaaatcatg
      901 ccgctgcttc gtgcagctgc tgcattcccc tgtgaaatgg ggcaggcaaa aaccaatccc
      961 gttgcgcggt tgaatgcttt tggagacaga gccagcgcaa accgcccgcc cttgatttcc
     1021 ttgccgctgg aagggtcgaa attcaaatgg aaaatatcgc ctttgtcggg aatatacatt
     1081 tcagacgacc tcgttgccgg catcatccaa gatttcccag ccttctacgc gcggcggggt
     1141 ttcttccatt tcggcaagca agtctgccaa gcggaaacgt cgggcagcac gcacacggag
     1201 ttcgccgtta tgtacttccg ctaccaaagc gtcgccgatt ttaaaatcca attgtttcag
     1261 catgtcggca ggcagtcgga cggccgccga gttcccccat ttttggacac gcaacataat
     1321 cttcaccttt attgtatcta caaagtagat acatattacc ataaaatttc agttgttcaa
     1381 atacttgtgc agaatacgcc aaaagccgtc cgaactgttt cggacggctt ttgtactgta
     1441 tttgcgcctt caggcaatat tttgttatcc attttcaaga tgcaaaagct ttctaattgc
     1501 ttgatgtcgg atttcggttg tttagggata caaaaccaag taaactaaaa ctgtttgatt
     1561 ggaaaatgct ccgcaaggag aaaattatgt tcaaaaaatc actttataag gctgctttgg
     1621 cgtatttcgg cgattgcgtg gctgcccata tttcagaaca gttttgactg tttattcaca
     1681 aatcagatgc ctttaggggg tttgctttcc ataatgcaac caaaatttcc aactctctaa
     1741 atattgtgtc tttgcgttct tcttcgcgta ttttcataac aagaggcgaa actgcattcc
     1801 acaattttat gaagttggtg caatgcagcc gtttaaacaa atcctcatcc aatactcccg
     1861 agttgattgc gcacgcataa aactcgtgcc gattgattac ggtaagtaaa tgccctctct
     1921 ctttttccca ttgtccgccc ttattttgat aaatttcata caggtcgatc cgcgcgttgt
     1981 tgtcgtcaac tgccgtgccg cgaatataag gcgaaatatg attgtcggct tctacaaatt
     2041 gtgcatcttg gtaatcattc aaaataacat ctaatgtcgc cttttgcttt gaagttttct
     2101 tattgatgaa tattgtcccc agtgcaatga cggcggttac tgaaacaacg gtcagttgcc
     2161 aaaacatcag ccattcggcc gtccctcata ggttaaactg tacgctgtgc ctgaatatgt
     2221 aaaataaagt gaatacagat acaaacaagg aggttagggc aattactgac aaccaacctc
     2281 cttgttttat ttgtttccgc accttaccag ccattaaaac cctcattttt gtatttcatc
     2341 gtatttcctt aatggtttta accttgcttg tcgatatctg tatattaatg ccgtgcgccc
     2401 gttctttcaa ggggtgattt aaaaatcagg catccttgac atcctctcct gcttaaaggc
     2461 ggggggactc ctgccgtgta aaccaatgcc gtctgaaggg ctttcagacg gcataaaaaa
     2521 accgcctttg tgtaaagcgg ttgaagaaaa gcctttcaat aaaaatgccg tctgaatttc
     2581 agacggcatt gttgtcggat atgcctattc cttatccagc cggcgcaggg tttcggcgag
     2641 ctgtttgaag tcggtttctc ccgccgccaa actgacaatc aagtcgtcaa gcccttggtc
     2701 ggcggcaatg ctgatgccct gcaaatccag ataggtcaac attgttaaaa gcgcggtgcg
     2761 cttgttgccg tcgggaaagg cgtgggcttt ggctatggct tgtgcataga gggcggcgat
     2821 ttcgtagatg tcctcaaggt tttcatactg ccgccagttg gcaatccgcg acaatgcgcc
     2881 gtccaagcgc gccatatccg cccgcccttt caaacccgct tcatccgcca atacggtttg
     2941 atggataagc gcgaccagtt cgccgtctat catttgtcgg caagtgcctt gacggctttt
     3001 tgatgggttt tggcaatgcg gcgggcggcg gcaagcagta cgcgtttgcc tgcttcgcct
     3061 ttaagctcga ttttgacggg gcgtgtattt tggttttgca tcgctgctcc ttgtctgttt
     3121 gcgggcattt tagctttttt ccggcagctt gggaaatgcc gtccgaaaac acttcagacg
     3181 gcattcttct aatagtgtaa tgcaatagtt actccaagat ttttgtatat ggctgttttt
     3241 atggtaaatt aaattttcat aagaaataaa tatatttaaa tcaatgaaat aaaatttagt
     3301 cgaatcccac cgtttccgcc acaaagcaaa accgccctga ttcggggcgg ttcttttttg
     3361 ttcaa
//
LOCUS       DAWIVB010000192         3294 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWIVB010000192 DAWIVB010000000
VERSION     DAWIVB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.192"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(184..405)
                     /locus_tag="WIX60_001879"
     CDS             complement(184..405)
                     /locus_tag="WIX60_001879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306098.1"
                     /translation="MLSTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQAEI"
     gene            464..1087
                     /locus_tag="WIX60_001880"
     CDS             464..1087
                     /locus_tag="WIX60_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ5306099.1"
                     /translation="MRKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1198..2580
                     /locus_tag="WIX60_001881"
     CDS             1198..2580
                     /locus_tag="WIX60_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ5306100.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2584..3108
                     /locus_tag="WIX60_001882"
     CDS             2584..3108
                     /locus_tag="WIX60_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ5306101.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          681 a          864 c          994 g          755 t
ORIGIN      
        1 aggattaaag gggtatttgg gtaaaattag gaggtatctg gggcgaaaac agccgaaaac
       61 ctgtgtttgg gtttcggctg tcgggaggga aaggaatttt gcaaaggcct caagatatag
      121 tggattaaat ttaaatcagt acggcaaggc gaggcaacgc tgtactggtt taaatttaat
      181 ctactatatt tccgcctgct gccgcccgaa aagcgtgatg cgctgatggt gctgctgcat
      241 aatccggacg acggataacc ggtgaaaacg gaaatgcccg ggcggtgttg cctgtgcctg
      301 tttggacaag cgttcttgag ggcggtagaa ttgaggtttg cccgaaaagg gcagggcgat
      361 atgccgcgct tggattttgc gcgcggtttt tcgcgttgaa agcaattatg tctgtttgaa
      421 tatttaatgc cgtctgaaac gtccggacgc gcccccgttc cctatgagaa aaccgaccga
      481 taccctaccc gttaatctgc aacgccgccg cctgttgtgt gccgccggtg cgttgttgct
      541 cagtcctctg gcgcacgccg gcgcgcaacg tgaggaaacg cttgccgacg atgtggcttc
      601 cgtgatgagg agttctgtcg gcagcgtcaa tccgccgagg ctggtgtttg acaatccgaa
      661 agagggcgag cgttggttgt ctgccatgtc ggcacgtttg gcaaggttcg tccccgagga
      721 ggaggagcgg cgcaggctgc tggtcaatat ccagtacgaa agcagccggg ccggtttgga
      781 tacgcagatt gtgttggggc tgattgaggt ggaaagcgcg ttccgccagt atgcaatcag
      841 cggtgtcggc gcgcgcggcc tgatgcaggt tatgccgttt tggaaaaact acatcggcaa
      901 accggcgcac aacctgttcg acatccgcac caacctgcgt tacggctgta ccatcctgcg
      961 ccattaccgg aatcttgaaa aaggcaacat cgtccgcgcg cttgcccgct ttaacggcag
     1021 cttgggcagc aataaatatc cgaacgccgt tttgggcgcg tggcgcaacc gctggcagtg
     1081 gcgttgattt tgaacccgcg ccgcaaccga aatacggcga atcctgtata atccgaaaat
     1141 ctgttcactg gaagttcaga cggcattgca actgttgatg ccgtctgaaa aaatatgatg
     1201 gcaagagaca accgcatcca aatgtttccg cacgaatggc gcgccagtac gacgctttcc
     1261 ggcgtgtacg cgctgcgtat gctgggtatg ttcctcgtgc tgcccgtatt ggcggtgtat
     1321 gccgcctcgc tgcccggcgc ggaaggcaac aaaacgctgg tcgggctggc aatgggcatt
     1381 tacgggctga cacaggctct gctgcaactg cctttgggca tcgcttccga caagttcggg
     1441 cgcaagaaaa ccatttatgc gggactggtc gtgtttgcgg cgggcagctt tcttgccgcc
     1501 gccgccgata cgctgcccat gctggtcgcc gcacgcgcca tacagggtgc aggggcggtc
     1561 agtgcggcgg ttaccgcgct gctggcggat ttgacgcgcg acggcgtacg tacccgcgca
     1621 atggcgatga tcggtttgag tatcggtttg acgttttcgg tcagcctcgt cgttgccccc
     1681 gtgattgccg acgcggtcgg cgttcgcgga ctgtttatgc tgaccggcat tctgaccgtc
     1741 atcagcatcg gcgtggtggc gtggatgact cccgatcccg aagtttccaa gctgcacgaa
     1801 gatacgcagg cgcagccttc gcgcataggc gaagttttga aaaaccgtag gctgctgacg
     1861 cttgatttcg gcattttcgc cctgcacgcc gcacaaatgg cattgtttac cgcgctgcct
     1921 ttcgcgatga cccagctcgg tttggaaaaa atacagcatt ggaaagtcta tctgccttcg
     1981 accattacgg gcttggtggt gatggttccg ctgattatcg tcggcgagac gcgcaacaag
     2041 cttaagcagg tttttgtttt gggtatcgtc tgtattgcgg cggcgcagct cggtttgctg
     2101 tccggtatgc gctcggtagg cttgattacc gcttatttgg ttgtttactt tatcggtttt
     2161 aatgtgttgg aagcgagcct gccgtctatg gtttccaaaa tcgcgccgtc cgacctgaag
     2221 ggtacggcga tgggcgtgta caacacgatg cagtcgctcg gactgtttgc cggcggcgcg
     2281 gcaggcggtt tgctgtttca aaaatacggc ttttccggcg tgtttgcctt ttgcagtata
     2341 ttgatgctgc tgtggctggt aattgccgtt ttatcgcctg cgcccaagcc cgtcaaaaac
     2401 ctcagttacc ctgtcggcgg cgtgtggcag ggcaatcagg aagggttata ccgcgccttg
     2461 tcggagcttg agggtgtgga agacatcggt ttcagtttcg acgggcagac cgtctatctc
     2521 aaagtgttgc agaagggttt cgatcaggct gccgctgaaa aaatcatcac aggagtttaa
     2581 aaaatgtcat tgaacaaagt catcctcatc ggccgcctcg gacgcgatcc cgaagtgcgc
     2641 tatatgccca acggcgaggc ggtttgtaat ttcagcgtcg ccaccagcga aacttggaac
     2701 gaccgcaacg gccaacgtgt agagcgtact gagtggcaca acatcaccat gtaccgcaaa
     2761 ctggcggaaa ttgccgggca atacctcaaa aaaggcgggc tggtttattt ggaaggcaga
     2821 atccaaagcc gcaaatacca aggcaaagac ggcatcgaac gcaccgctta cgatattgtc
     2881 gccaacgaaa tgaaaatgtt gggcgggcgc aatgaaaaca gcggcggtgc gccttacgag
     2941 gaaggttacg gtcagagtca ggaggcttac caacgccccg cgcagcaaag ccggcagccc
     3001 gcctccgacg cgccgtccca tccccaagaa gcaccagccg cgccgcgccg ccaacccgtg
     3061 cctgccgccg ccccggtcga ggacattgac gacgatatcc cgttctgaat tttacggccg
     3121 gacatcctcg cggagggaaa tcataaagga cggagaaacc ttaaacctta cggggcaggg
     3181 tttctctttt ttgcatttgg gccggcttgt tgccgccctt tgcgttgggc ggaacgttgc
     3241 gattgcgccg ttcgggcgtt ttgaggcagt gatgcctgcc gcacgtcccc gctg
//
LOCUS       DAWIVB010000193         3293 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWIVB010000193 DAWIVB010000000
VERSION     DAWIVB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.193"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            83..1096
                     /locus_tag="WIX60_001883"
                     /pseudo
     CDS             83..1096
                     /locus_tag="WIX60_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1312..2649
                     /gene="ribB"
                     /locus_tag="WIX60_001884"
     CDS             1312..2649
                     /gene="ribB"
                     /locus_tag="WIX60_001884"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ5306102.1"
                     /translation="MTDTAGLRRHNLRQWIEKYYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPSEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALEHIQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2809..2946)
                     /locus_tag="WIX60_001885"
     CDS             complement(2809..2946)
                     /locus_tag="WIX60_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ5306103.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(2957..3187)
                     /locus_tag="WIX60_001886"
     CDS             complement(2957..3187)
                     /locus_tag="WIX60_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ5306104.1"
                     /translation="MEDFSVVIGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKM
                     AGFSADLDKLFAESAELEKEIKDRLAMLKFNS"
BASE COUNT          860 a          901 c          735 g          797 t
ORIGIN      
        1 ttacagggtg cggatgcaaa cgcattgcga gcgcgggttt gaggcatacg cgcaaacatc
       61 ttaatataat ggattgatat ttatgatttt ctccatcatc gtccctattt acaatgtgga
      121 aaatacctcc gctgctgcgt ggattccgtg cttgccgaaa attttgccga ttatgaaatg
      181 attttggtcg atgacggttc gccggacggc tgcgggaaga tttgcgacga atatgcaggc
      241 aaatatccgc atataaaagt gattcatcaa gaaaacggcg ggctgtcgga tgcccgcaac
      301 gccggtatcc gggcggcaaa aggcgattac ctaatctttt tggacagcga cgattattgg
      361 gccgatacca accgttcaaa aaacgcgggg gggggggatt ctctttgatt tacaacaact
      421 tgcagacaaa aaggttgatt tgatcctgca tccctcgtcc ttcaattacc gcgacatccc
      481 caaaggggcg gacttttcgg ataatgattt tgtccgccat tttgaaacgc tggtggaggg
      541 gcggtactat atcgccaacg cgtggacaaa gattgtcagg cgggaaatca tcattaaaaa
      601 caatctgttt ttcccaaaag gatacattca cgaggatttc ccgtacagtt tgcaattggc
      661 gcgttttatc aagacttttg ccttttacga taaccctttt taccagtacc gcgttctcgg
      721 cggctctatc agccacaaca tcaaatacaa aaatttcagc gatgtgctga cgcatctcga
      781 ctggggtgtg gattttttag tcgaaaacaa aaattccccc atctacggcg gtttgcaaaa
      841 atttgtcttc gacaatatcg gctatctgag gtctatattg gtaaggcttt atttttccaa
      901 aaacattatc ctcatctacc ggaaatattt ttcatttaaa gaaaaatgca gaaagatatt
      961 cggcgcgaag gcaatccgtc cggtttttat cgggaagacc gcattcatca taggattgcc
     1021 gatattgcgc ctgctcgtac cgcctatgct gtacccggca atcaaggccg tttatcagaa
     1081 atttttttcg gaataagctt ccggcaaacc ccgaatcgga agcgggcggg aagaaacagc
     1141 cgccccgccg gcggggattg cggcaatgcc gtctgaagcc acgaatccgg cttcagacgg
     1201 catctgttta ccaaaaagca aataattcgg tttggcgaaa aaaacagatt tgctttttgg
     1261 taaatacgcg attacaatcc gctacatccg atttctacaa aggatgaaac gatgaccgac
     1321 acagccggtc tgcgccgcca caacctgcgg cagtggatag aaaaatacta cggcggtttg
     1381 caaactcgtt ttgctgaagc cgttgccctc aacacaggcg aactctccgc ccttttgaaa
     1441 aacaaatcct tcggcgagaa aaaagcccgt aaaatcgaac aggcggcaaa aatgcccgcc
     1501 ttttggctcg ataccgaaca caccgcccgc ccgtccgaac acacaggaaa acacaccatg
     1561 tcccatatct cccccatccc cgaaatccta gccgacatca aagccggcaa aatggtcatc
     1621 atcaccgatg ccgaagaccg agaaaacgaa ggcgacctgc tgatggcggc gcaattcgtc
     1681 acgcccgaag ccatcaactt catgatcaaa cacgcgcgcg gcttggtctg cctgccgatg
     1741 gacggcgaaa tggtcgaaaa actcgggctg ccgatgatga cccaaaaaaa cggcgcgcaa
     1801 tacggcacca actttaccgt ctccatcgaa gccgcacacg gcattaccac cggcatttcc
     1861 gccgccgacc gcgccctgac tattcaaacc gccgtttccc cgaccgctaa acccgaagac
     1921 atcgtccaac ccggtcatat ctttccgctt cgcgcccaaa aaggcggcgt actcgtccgc
     1981 gccggacaca ccgaagccgg cgtcgacctg gcgcaaatga acgggctgat tcctgcctcc
     2041 gttatttgcg aaatcatcaa cgacgacggc acgatggcgc gtatgcccga actgatgaaa
     2101 ttcgccgaag aacacaagct caaaatcggc acgattgccg acctcatcga ataccgcagc
     2161 cgtaccgaaa gcctgcttga agacatgggc aatgcgcctg tacaaacccc gtggggcgag
     2221 ttccaacaac acgtttacgt cgacaaactc tccggcgaaa cccacctcgc cctcgtcaaa
     2281 ggcacgcccg ccgccgacac cgaaaccctc gtccgcgtcc acgaaccctt cagtgtgatg
     2341 gacttcattc aagccaaccc gcgccattca tggtcgctgc ccaaagccct tgagcacatc
     2401 caacaagccg aaagcggcgt cgtcatcctc ttacaccgca ccgaagacgg cgcatccctg
     2461 ctcgaccgaa ccctacccaa aggcgcaaac caagcctaca aatgggacag caaaagctac
     2521 ggcatcggcg cacaaatcct cgccggcctc aacgtcaaaa aactgcgcgt cctcgggcag
     2581 ccctcatctt tcaccggcct gaccggcttc ggtttggaag tcgtcggctt tgaagaagcg
     2641 gaaaaataat atagtaaatt caaatacttt atatttgctt tatttattgc attatttccg
     2701 tgcaaacgaa aacccggtct gttgggttgg attttgtttt ttcaaatttc gggtaacttc
     2761 taattcgtca ttcccgcgct ggcgggaatc cggttcgtcg ggtttttgtc atttccgata
     2821 aattcctgtg gctttggttt tttggattct ctctttcagg gaaagaacgg cataagtatt
     2881 ttccaaacca aacaaaatgc cgtctgaaag gctttcagac ggcattttaa gtttgaccgg
     2941 tttcattcat cggtatttat gaattgaatt tcaacatcgc caatctatcc ttaatctctt
     3001 tttccaattc ggcagattcg gcgaaaagtt tatccaaatc cgctgaaaat cccgccattt
     3061 tttgcgcaaa ttcgtcggcg gaaatatcca cataatcaat ctttacctcg aaatactgcc
     3121 ccgccgacaa gctgtgattc ttcgctttga tttcatcgta gccgattacc acactgaaat
     3181 cttccactgc ctgtttgtgc gtgaaggtat tgcagatttt tttgttcttc ttcgcgggaa
     3241 agtacggttt ttttgccgtc tttaattttt tcgcccaagc ccgatgcgtc gat
//
LOCUS       DAWIVB010000194         3278 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWIVB010000194 DAWIVB010000000
VERSION     DAWIVB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.194"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..941)
                     /locus_tag="WIX60_001887"
     CDS             complement(<1..941)
                     /locus_tag="WIX60_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ5306105.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTRSHKIDQKEFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDGEGNWSDVGLSSASFLGGLMGVQWGAALGVGAFGLLTGGAGWLVVGA
                     ILGGGAGAWWGSLGAEKAWRWFEDFLNLNRSDKYHVYDPLALDLDGDGIETVATKGFA
                     GALFDHRNQGIRTATGWVSADDGLLVRDLNGNGIIDNG"
     gene            complement(1498..1947)
                     /locus_tag="WIX60_001888"
     CDS             complement(1498..1947)
                     /locus_tag="WIX60_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306106.1"
                     /translation="MQPGSKNDEKNKITLNEFNNAIVTIFSDENVVRAIGTINTMHGE
                     YLTRSHKIDQQKFLSDGERLSSKAKDFQHSAEKTQRALKEGIGRAASKFHANSKEAFV
                     ARKMQSDLQKKLNASNYNKQQYARQAVTALENASKAENKGNIAFYLN"
BASE COUNT         1115 a          667 c          458 g         1038 t
ORIGIN      
        1 ccgttgtcga tgatgccgtt gccgttcaaa tcgcggacga gtaaaccgtc atcggcagaa
       61 acccaaccgg tggcggtgcg gatgccttga ttgcggtggt cgaacaatgc acctgcaaag
      121 cctttggtgg caacggtttc tataccgtct ccgtccaaat ctagggcgag ggggtcgtaa
      181 acgtggtatt tgtcgctgcg gttaagattt aagaaatcct caaaccatct ccaagctttt
      241 tcagctccta atgatcccca ccaagctcct gcgccacctc caagtatagc tccaactact
      301 agccaacctg cgccaccagt aagtaaacca aaagccccaa cacccaaagc cgcaccccat
      361 tggacgccca ttagcccacc caaaaaagaa gctgatgaga gaccaacatc actccaattt
      421 ccttctccat ccaaggcttt ccaccaagca cttcccatac tcaaagttcc catggcaaca
      481 gttgccccat aagtaaactt acccaaattc gctgccataa cttttgattt tgaagcattc
      541 tctaacgctg ttgccgcacg tcttgcatat tgctgcttgt tatagttgct agcattcaat
      601 gctttttgca aatcactttg tacttttctt gcaacaaatg cctcctttga attaccacga
      661 aatttactag cagctcttcc aattccttct tttaagcttt tttgtgcctc ttctgctgtg
      721 cgttggaatt ctcttgcctt agcagacaga cgctcaccct cccttaaaaa ttctttttga
      781 tctatcttgt gcgaacgggt caaatattct tcgtacattg agttaatcgt accaatcgcc
      841 ctaaccacat tttcatcaga aaatatggaa ataatagcat ccctatacgc acctagtgta
      901 atattgtttc tattattagt tatagcatta ttcgaataca taatagcacc tccaaattta
      961 gaactaaata cgtttatatt ctctgtgtaa taatatcaaa taattaatta atggataagt
     1021 aagtccgaaa aaataacaac taaaataagc aatatactga ttctctcctt tattaattag
     1081 atatattgaa aaacaaataa aaaataaaca gaaaacattc atgtgaaaaa gccacctcat
     1141 attcttattt ataagtttaa aataactagg cattgtatat ttaaaaatcg gcacatccaa
     1201 cggaaacaaa ccacataaaa atttttcttt attctttatt aaagacatag ttttattttt
     1261 taaaattagt ccaaataata tactaacaat tactattaaa acaataagca cagcgtaatt
     1321 ttttataaaa ttcattaaaa taataacgaa aatactaaat agattacata ctataaattg
     1381 aaatataaaa tattcagtat ttaatccatt ttcaaccgcg agttttttaa aatatccttg
     1441 ctcgaaaaaa ttaaaaaaat ccattaatac tttcctctca agaaaactaa ttttagttta
     1501 gttaagataa aatgcaatat tacctttatt ttctgctttt gaagcatttt ccaaagctgt
     1561 gactgcttgt cttgcatatt gttgcttgtt atagttacta gcatttaact tcttttgcaa
     1621 atcactttgc atttttcttg caacaaatgc ctccttcgaa ttggcatgaa atttactagc
     1681 agcccttcca atcccttctt ttaaagccct ttgagttttt tctgctgagt gctgaaaatc
     1741 tttcgcttta ctagacaacc gctcaccatc actcaaaaat ttttgttgat ctattttatg
     1801 cgaacgagtt aaatactccc catgcattgt gttaattgta ccgattgccc taaccacatt
     1861 ttcatcagag aaaatagtta caatggcatt attgaattca tttagtgtaa tcttattttt
     1921 ttcatcattc ttagaaccgg gttgcatgac aacaccttcc atcaactaaa atattatatt
     1981 attaagagac tacaacttag atacgtttat aatctctgga taaaaaaacg taataagtgt
     2041 ttgctggata acaaagtcca aaccaatagc agataaaata aggcatccac cccctttctt
     2101 cattaaggat cagagatatc gagaaacaaa tcataactaa acagaaaaaa ttttggagat
     2161 aaagccattt cattccccta ttcaagaatc tggccaagat aggtattttg tatactctag
     2221 aaaagaaagg catttccaag ggaaacatat cagataaaaa cttttgttta ttttttacta
     2281 tagatagaac cctatttctc aagataaatc caaatgcaat gccaattatc attattaata
     2341 aacaaaggat agtataacta tggataacct tcattaaaca aaaagaagaa atggcagata
     2401 aattacatac gatatagtgg aatataaaat atttacggtc taaaccttgt tcagttgcaa
     2461 tttttttata cttaccttgc gcaaaaaaaa tcaaagaaat ccattaaact atctttcaca
     2521 ttagaaattt aaaagctaaa taatacgaca aacaatgtga agtactattc atggtttatt
     2581 taaaaaataa tactattctg aacattattt agatacagaa attaacaaat tggaactaaa
     2641 caagctttta aatactttaa ttttattgga aagctataaa aggaactata gtttcacaca
     2701 ctaattactt cctaatcact tctttattaa gaggcacaag ggattgggga ggctgtcttg
     2761 gagataagca ctgatgttct aggcaaaccc ctccatctta aagttaaaac aaccacaggc
     2821 agcctggaaa tgatctatga tctatgccca tacaaggaca ttctagatag ttttccttgt
     2881 tgcttcgtgt atcacacaag atagaattgg cactctactc tttccttaaa aaaccgtcaa
     2941 gaatggttat caattttttt aaacttattc aaaacaatat tcttatacac atctcaaacc
     3001 cgctgaaata aagcaaagtg gtggttcata gatggcaata ccgatttcaa agcagcctgc
     3061 actttgacaa cacacgagaa atacgggaag aacagggcat cagcatatat ttcagcccga
     3121 agccgtttca ttcgtttaaa aaactgcttt tttcacaatt tacctttaca aagcctatac
     3181 gtatccttac aatttgtttt gctgtttttg gtatattttt ggggctgtac tacattagca
     3241 gatatgttac cctcgaaata tgaagataac acactgca
//
LOCUS       DAWIVB010000195         3251 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWIVB010000195 DAWIVB010000000
VERSION     DAWIVB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.195"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(653..1090)
                     /gene="rnhA"
                     /locus_tag="WIX60_001889"
     CDS             complement(653..1090)
                     /gene="rnhA"
                     /locus_tag="WIX60_001889"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ5306107.1"
                     /translation="MNQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTASKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1204..2073
                     /gene="tehB"
                     /locus_tag="WIX60_001890"
     CDS             1204..2073
                     /gene="tehB"
                     /locus_tag="WIX60_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ5306108.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGSMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2123..2929)
                     /gene="thiD"
                     /locus_tag="WIX60_001891"
     CDS             complement(2123..2929)
                     /gene="thiD"
                     /locus_tag="WIX60_001891"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ5306109.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLEFDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCEAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            2990..3187
                     /locus_tag="WIX60_001892"
     CDS             2990..3187
                     /locus_tag="WIX60_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306110.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
BASE COUNT          677 a          789 c          951 g          834 t
ORIGIN      
        1 tttaatttgt ctgtccgcat tgccgctaat tgtaccactt catcgtattt ccttttcggt
       61 tgaaaccccg ccctttaggg cggcaggatc agactttatt tgggaggggg tgtaacccct
      121 tccaaatcag gacgacacat agggaggtgc tttatgtgtc gtcctgtgtg ttgaaacata
      181 tcggacgcgt ttgaacagtt gtgcggcaaa ctgttttagc ggcaggcaag agatgccgtc
      241 tgaagcccat tgtccgtcat tcccacacag gcagttgcta cggttgctga agccccttag
      301 ccgtcgcgca ggcggttgct acggttacta aaatcccgtc attcccgcgc aggcgggaat
      361 ccagtccgtt cggtttcagt tgtttctgat aaatcttgca gcgttgggtt tttagattcc
      421 cgctttcgcg ggaatgacgg ggcggtcgga tgccgcctga aactccgtca ttcttgcaac
      481 ttttcgtcat tcccgtgaaa acgggaatct agaacttcaa actttcagat aatctttgaa
      541 tattgccgtt gcccgacggt ttggattccc gcttgcgcgg gaatgacggt ggaggacgat
      601 gccgtctgaa gcccattagc ggtttcagac ggcatttttg ccggagcggc agtcaggaaa
      661 actgcgctgc gccacggttt gccaaatcgt cggcgcgttc gttttcggcg tgtcccgcgt
      721 gtcctttcac ccaagtccaa ctgacttgat gccgtccgac tagagcgtcg agttctttcc
      781 acaagtcgtc gtttttgacg ggctgtttgg aggcggtttt ccagccgttg cgcttccaac
      841 cgtgtatcca gttttccatg ccatttttga cgtattgcga gtcggtgcag atgatgacgg
      901 tgcagcggcg tttgagcgat ttcagtcctt cgatgacggc agtcagttcc atgcggttgt
      961 tggtggtttg cgcttcgccg ccgaaaagtt ctttttcgtg gctaccgtag cgcattaaca
     1021 cgccccagcc gcccgcgccg ggattgcctt tgcacgcgcc gtcggtgtaa aggtaaacgg
     1081 tttggttcat tattgtgcct ttgtgtggtg tccggcgtga ttataagcgg tttgcgcgcc
     1141 gtgccgtctg aaagattgcg gcggattaaa ataggcatat aatgtgcaac tttatttttg
     1201 aatatgaagg agcggatagt gggtcaaagt ggcgagttgt tttgtttcgg acagatgcct
     1261 gtatggaaag tggaaaacct gccggaagtt ttgttgtcgg gctattcgtc tgaggaaggg
     1321 gagtgggtct gcctgaatgt gttgcagggc gatgtcgaag ttcgtgcacc ggacgggtcg
     1381 gcagaagttt ggtcggcaga cggcggcgat tgtgtgtttg cgccgcagca ggtgttttct
     1441 gtcaaaccga aaacggacga tgccgaaatc cgtttgtcgc tgtattgcgc ggcggcagac
     1501 tattttcaca aaaaatacgg gatgagtgcc acgcattctg cggtcgcggc ggcacgggat
     1561 accgtaccgg cgggcagggc gttggatatg ggctgcggac aggggcgcaa cgcgctgttt
     1621 ctcggtttga aggggtttga agttactgca gtcgatcaca atcccgccgc cttggcaaac
     1681 gtggcggagc tggcagaggc ggagggtttg aacgtccgca cgctggaata tgatttgaac
     1741 gccgccgcct tgcagggcga gtttgattat attgtggcaa cagtggtgct gatgttcctg
     1801 atgccgcagc gcgtacccga cgtgattgcc gatatgcagg cgcatacggc ggcgggcggg
     1861 tacaacttga ttgtatcggc aatggatacg gcggatttcc cctgtccgat gccgttccct
     1921 tttaaattta aagagggcga gctgaaggat tattatcggg attgggaact ggtcgaatat
     1981 aaagaagaat tgggttctat gcatgccaaa gacgaaaacg gcaatccgat acggtttaaa
     2041 tttgtaacga tgctggcgaa aaagcctgaa taatcctcaa aaatgccgtc tgaacatttt
     2101 gtttcagacg gcatttttac ggttagtccc gatacgccca atgattgacc gggccgtgtc
     2161 ctgcgccgat ttccaaaggg tttgagattg ccgccgtgat gtaggccttg gcagtctgta
     2221 cggcttcgca aacgtccgag ccttttgcca actcggcggt gatgcaggcg gaaaacgtgc
     2281 agcccgtgcc gtgcgtgtgg gcggtcggaa agcgcgggct gtcgaattcc agcgtttcat
     2341 tttgtgtaaa cagccaatcc gtgcagcgtc cgcttgtgct gccgttcaaa tgtccgcctt
     2401 tgataacgac atttttgaca ccgtaatcaa gcaggatttt tgccgcacgt tccgcatctt
     2461 tacggttttc aatatgcacg ccggtcagag cttccgcttc gggcaggttg ggggtcaata
     2521 catccgtatc gggaagcagc aggcgcgtca gtgccgcaac ggcggaatcc tgcaacagcg
     2581 gcgcaccgcc tttggcaatc atcacagggt cgagtacgcg cctgccaaag ctgcagtgtt
     2641 tcagcttgtc ggcaacgcat tcgatgattt ccgccgtgcc gagcataccg attttgtagg
     2701 cgcggatgtc gaagtcttcc ctgattgctt ggatttgtgc ggtgatggtt tcggtcggga
     2761 cgagatgaac cgccgacacg cccaaggtat tttgcgcggt aacggcggtg atgacgcacg
     2821 ttccgaacac gccgcgcatc tgaaatgttt tcaaatccgc ctgaatgccc gcaccgccgc
     2881 ccgaatccga accggcgatg gtaagcgttt gcacaaaaga gcttttcatt tgttcctcct
     2941 taatattgcg tccgataaaa agggctaatg cctgtaaaaa agcacggcga tgccgtctga
     3001 aaaccgcagc gcgggggttt gtggtgcatt atatgccgta ttgcaggaca aggcacaggg
     3061 cagggcggcg gggtgcatcg tagcaactgt atttttgccc gacggggtga aaaatacagt
     3121 tgctacaccc actgataatg aaaaaagaga gcaatgccgt ctgaaccgcg tgttgcggcg
     3181 agcataaacc ggcacgcttt attcgtcatt tccacaaaaa cagaaaccta aaattccgtc
     3241 attcctacga a
//
LOCUS       DAWIVB010000196         3188 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWIVB010000196 DAWIVB010000000
VERSION     DAWIVB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.196"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(75..668)
                     /gene="ribA"
                     /locus_tag="WIX60_001893"
     CDS             complement(75..668)
                     /gene="ribA"
                     /locus_tag="WIX60_001893"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ5306111.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(661..1338)
                     /locus_tag="WIX60_001894"
     CDS             complement(661..1338)
                     /locus_tag="WIX60_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306112.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            1492..1576
                     /locus_tag="WIX60_001895"
     tRNA            1492..1576
                     /locus_tag="WIX60_001895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Leu,seq:tag)
     gene            2096..3130
                     /gene="purM"
                     /locus_tag="WIX60_001896"
     CDS             2096..3130
                     /gene="purM"
                     /locus_tag="WIX60_001896"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ5306113.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGVGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT          806 a          847 c          793 g          742 t
ORIGIN      
        1 ttattgcttt atcaaggtat ggaaacctgt ttcccgaaag gcggcgcagg atgcccgttc
       61 cctgcaactt tgccttattc cgacatcaga tgtcccagct tgtctgcttt ggtttggagg
      121 tagcgtttgt tttcaaggtt ttccccgacg tgcaggggga tgcgttcgac cacgttaatc
      181 cccgcatctt tcagggtttg gattttttcg gggttgttgg tcagcagttt gaccgagcgg
      241 atgcccagat attcgtagat agattgcgcc aaacggaaat cgcgggcatc gacgggcagc
      301 ccgagtgcca aattggcttc aaccgtatcc ataccttggt cttgcagatg ataggcgcgg
      361 attttgttaa tcagcccgat gccgcgtcct tcctgacgca gatagacgat gatgccgcgc
      421 ccctctgtct gtaccgccct catggccgct tcaagttgag gtccgcagtc gcattttctc
      481 gagaacagcg catcgcccgt caggcattcg gagtggatgc gcgtcagaac cggattgccg
      541 tctgaaaaat taccgacggt cagcgcgacg tgttcctgcc cgtttgcctc ttcaaagccg
      601 tgcatcgtaa atacgcccca ttcggtcggc aggcggcagg atgccacatg gttcaacatt
      661 tcagacatct tcactcccat cttttccggc agacataccc aacagcagtt tcaaaggttc
      721 ggacaaagcc aaggcaagag ccacccattc cacctgtgct gccgtacccg cacactctgc
      781 gcattcaaat tccacatgaa ccacgcccaa cacaccgcca ctttccgtgc agaccggaat
      841 ggaaatttgc gccgccgaag catgattgcg ttctcccgaa agctccccca aatccaacca
      901 acggcgtaca tccgaggcaa cattcatcca accgctttgc gccgaacggc aagccagcga
      961 aaccttgcct gccgcttcat ccaaatccca caggttttcc aaaacctcgc cctgtcggga
     1021 caggcatatc agttggaaag cctggttttc agatggcatc aaggcataga ccgccgcact
     1081 ccgcacgcct gtcgagcgcg aaaacaccga atccaaagcc atgaaaagcc gtttcagtgc
     1141 atcttcgcgg acattgtcgc acgacaggta atcggcaagt ttccaaccct catcactgcg
     1201 ccacaaaacc gaacggtcta tcgaagccgt ccccatatcc attaccgtct gcgccgtcag
     1261 atacgccgcc cgaacctcgt caagcggcag cttcaaaccc tgagtcagca gaaaatcctt
     1321 aatcaaagca gcaggcatac ccaaattccg tcaataaaaa caaaaaaccg cccgattccg
     1381 atgtcaggcg gacgaaaaac agattttacc gccccaaacc ccaagccatc aacagccccg
     1441 ccgaaaacct tgcacaaaaa aacaaaatct gtataatcgt ccaattccca tgcggacgtg
     1501 gcgaaattgg tagacgcacc agatttaggt tctggcgccg agaggtgtga gagttcgagt
     1561 ctctccgtcc gcaccaataa tgataaataa attatatagt taatgcttat cgggacaaat
     1621 tagtgccaca aaacataagt cgtctgcttt tcagcgggcg gcttttttat ctgcctatat
     1681 gtttcaacac gcagaacggt acataaagtg ccgccatttg ttccgcaccg aatcggaaac
     1741 gggtgcttcc gcacgcaatc agattcacca cccctaatgc cggggtttca accggaaaga
     1801 agaaaatatt gattttgatg atggcatcaa aaaacccctt ccgctcgtct gccaaaagga
     1861 atggttaaga aaagcaatgc gtcttttgat ggaacgcgat atataaggaa ttgaggggaa
     1921 gggcaggcgg gtttctgccc gttaatcctg cggacggtcc cgccaaaccc gacacgcgcg
     1981 ggcatatggc ggactgttta caatattgtc taaaaatctg aacctgcaaa cgtgaaataa
     2041 ggtagaatac gccccgttat tttaccgtcc aatccccatt taccaaggaa aaacgatgag
     2101 tacttcattg agttaccgcg atgcaggtgt cgatatcgac gcaggcgacc aactggtcga
     2161 aaacatcaaa ccgtttgcca aacgcacgat gcgcccggaa gtattggggg atttgggcgg
     2221 ttttggcgca ttggtcgaaa tcggcaagaa atatcaaaat cccgtattgg tcagcggtac
     2281 ggacggcgtg ggtaccaagc tcaagcttgc ctttgattgg gataaacatg atacggtcgg
     2341 catcgacctt gttgcaatga gtgtcaacga tattttggtt caaggggctg agcccttgtt
     2401 tttcttggac tattttgcct gcggtaaatt ggatgttccg cgcgcgaccg atgttattaa
     2461 aggcattgcc caaggttgcg aagaatccgg ttgcgccctg attggcgggg aaactgccga
     2521 aatgccgggt atgtatcccg tcggagaata cgatttggcg ggttttgccg tcggcgtggt
     2581 ggaaaaagag aatgtcatta ccggccgcag catcggcgta ggcgatgtgg tattgggttt
     2641 ggcttccaac ggcgcacatt caaacggcta ttcccttatc cgtaaaatca tcgaacgcga
     2701 caatcccgat ctggatgccg agtttgataa tggcaaaacc ttgcgggagg ctgttattgc
     2761 gccgacccgt ctgtatgtga aaccgattct tgccgctttg gaaaaattta ccattaaagg
     2821 tatggcacac attaccggcg gcggcattac cgaaaacgtg ccgcgcgtgt tgcctgaaaa
     2881 cacggttgca caaatcgatg ctaaatcgtg ggaattgccc aagctcttcc aatggcttca
     2941 aaaggcgggc aatgtggaaa cccaagaaat gtaccgaacc tttaactgcg gcatcggcat
     3001 ggtcgttatt gttgctgccg aagatgccga tgcggttcag ggtctcttgg gtgaacaagg
     3061 tgaaacagtt taccgtttag gtttgatccg tgagcgtcag ggagacgagc atcaaaccca
     3121 ggttgcctga ttgcttctat agcgaaatgc cgtctcaaac atgggtttgg acggcatttt
     3181 tatagagg
//
LOCUS       DAWIVB010000197         3119 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWIVB010000197 DAWIVB010000000
VERSION     DAWIVB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.197"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..507
                     /locus_tag="WIX60_001897"
     CDS             <1..507
                     /locus_tag="WIX60_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEZ5306114.1"
                     /translation="SMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQ
                     LAVFGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEI
                     IRAFEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVS
                     NNPNGYDD"
     gene            623..1690
                     /gene="rfbB"
                     /locus_tag="WIX60_001898"
     CDS             623..1690
                     /gene="rfbB"
                     /locus_tag="WIX60_001898"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ5306115.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIRNTRDAVVNVDKLTYAGNLESL
                     TEVADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRSIGSAGEFIQTNI
                     VGTFNLLEAARAYWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPE
                     KPAGVARYEDLITFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDN
                     KTWWQNVLNGSYRLERLGTGK"
     gene            1753..2619
                     /gene="rfbA"
                     /locus_tag="WIX60_001899"
     CDS             1753..2619
                     /gene="rfbA"
                     /locus_tag="WIX60_001899"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ5306116.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            2673..>3119
                     /gene="rfbC"
                     /locus_tag="WIX60_001900"
     CDS             2673..>3119
                     /gene="rfbC"
                     /locus_tag="WIX60_001900"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ5306117.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYKPEAEQVLIWNDP"
BASE COUNT          779 a          836 c          781 g          723 t
ORIGIN      
        1 agcatgattt tgttgcgtta tttcaatccg attggcgcgc atgaaagcgg cttgattggc
       61 gagcagccaa acggcatccc gaataatttg ttgccttata tctgccaagt ggcggcaggc
      121 aaactgccgc aattggcggt atttggcgat gactacccta cccccgacgg cacggggatg
      181 cgtgactata ttcatgtgat ggatttggca gaaggccatg tcgcggctat gcaggcaaaa
      241 agtaatgtag caggcacgca tttgctgaac ttaggctccg gccgcgcttc ttcggtgttg
      301 gaaatcatcc gcgcatttga agcagcttcg ggtttgacga ttccgtatga agtcaaaccg
      361 cgccgtgccg gtgatttggc gtgcttctat gccgaccctt cctatacaaa ggcgcaaatc
      421 ggctggcaaa cccagcgtga tttaacccaa atgatggaag actcatggcg ctgggtgagt
      481 aataatccga atggctacga cgattaagtt gacctgatac aggccgtctg aaagagatgt
      541 tttcagacgg cctctttatc tgaaaaacac acattctgtc tgctataatc tgtttatatt
      601 ttttggctat cctctgaaat ttatgagaaa aatccttgtt accggcggcg cgggctttat
      661 cggttctgcc gttgtccgtc atattatccg aaacacccgg gacgctgtcg tcaatgtcga
      721 taagctgact tatgccggca atttggaatc tttgactgag gtagccgata atcctcgcta
      781 tgcttttgaa caagtggata tttgcgaccg cgccgaactc gaccgcgtat tcgcgcaata
      841 ccggcctgat gccgtgatgc acttggcggc ggaaagccat gtcgaccgct ctatcggttc
      901 ggcaggcgag tttatccaaa ccaatatcgt cggcacattc aatctgcttg aagcagcccg
      961 cgcctactgg caacaaatgc cgtctgaaca gcacgaagcc ttccgtttcc accatatttc
     1021 caccgatgaa gtctatggcg atttaggcgg cacggacgat ttgtttaccg aaaccgcgcc
     1081 ctacgcgccg tccagcccct actctgcctc taaagcgtcc agcgaccacc tcgtccgcgc
     1141 gtggttgcgt acttacggct tgccgaccat tgtaaccaac tgctccaaca actacggtcc
     1201 ttaccatttt ccggaaaaac tcattccttt gatgattctg aacgcgcttg acggcaaacc
     1261 gctgcctgtg tacggcgacg gtatgcaaat ccgcgactgg ctgtttgtcg aagaccacgc
     1321 gcgcgcactg tatcaggttg ttaccgaagg tgttgtcggc gaaacctaca atatcggcgg
     1381 ccacaatgaa aaagccaata ttgaagtcgt caaaaccatc tgcgccctgc tggaagaact
     1441 cgctcccgaa aaaccggccg gtgtggcgcg ttatgaagat ttgattactt tcgtacaaga
     1501 ccgccccggc catgacgtac gctacgccgt cgacgcagcc aaaatcaggc gggatttggg
     1561 ctggctgcct ttggaaacct tcgagtccgg cctccgcaaa accgtgcaat ggtatctgga
     1621 caacaaaacc tggtggcaaa atgtattgaa cggcagctat cgtttggaac gtttaggtac
     1681 tggaaaatag ttttcagacg gcatcccgac gcaatgccgt ctgaaaaccc atcgcaaagg
     1741 aagaaagaaa agatgaaagg catcatactg gcaggcggca gcggcacgcg cctctacccc
     1801 atcacgcgcg gcgtatccaa acagctcctg cccgtgtacg acaaaccgat gatttattac
     1861 cccttgtcgg ttttgatgct ggcgggaatc cgcgatattt tggtgattac cgcgcctgaa
     1921 gacaacgcct ctttcaaacg cctgcttggc gacggcagcg atttcggcat ttccatcagt
     1981 tatgccgtgc aacccagtcc ggacggcttg gcacaggcat ttatcatcgg cgaagaattt
     2041 atcggcaacg acaatgtttg cttggttttg ggcgacaata ttttttacgg tcagtcgttt
     2101 acgcaaacat tgaaacaggc ggcagcgcaa acgcacggcg caaccgtgtt tgcttatcag
     2161 gtcaaaaacc ccgaacgttt cggcgtggtt gaatttaacg aaaacttccg cgccgtttcc
     2221 atcgaagaaa aaccgcaacg gcccaaatcc gattgggcgg taaccggctt gtatttctac
     2281 gacaaccgcg ccgtcgagtt cgccaaacag ctcaaaccgt ccgcacgcgg cgaattggaa
     2341 attaccgacc tcaaccggat gtatttggaa gacggctcgc tctccgttca aatattggga
     2401 cgcggtttcg cgtggctgga caccggcacc cacgagagcc tgcacgaagc cgcttcattc
     2461 gtccaaaccg tgcaaaatat ccaaaacctg cacatcgcct gcctcgaaga aatcgcttgg
     2521 cgcaacggtt ggctttccga tgaaaaactg gaagaattgg cgcgcccgat ggcgaaaaac
     2581 caatacggcc aatatttgct gcgcctgttg aaaaaataat gtttgaggcc gtctgaaact
     2641 tttcagacgg cctttagatg aaagataaaa agatgaacat cattgatacc gccattcctg
     2701 acgtaaaact gcttgagccc caagtcttcg gcgacgcgcg cggctttttt atggaaacct
     2761 tccgcgacga gtggtttaaa acccaagtct gcgaacgcac cttcgtgcag gaaaaccact
     2821 ccaaatccgg caaaggcgta ttgcgcggcc tgcactatca aactgaaaac acacaaggca
     2881 aactcgtacg cgtggttgtc ggcgaagtat tcgacgtggc cgtcgatatg cgtaaagact
     2941 cccccacttt cggcaaatgg gtaggcgaaa ttctgtccgc agaaaacaaa cgccaactgt
     3001 gggtacccga aggtttcgca cacggcttct atgtactgag cgatgaagcc gagttcgtct
     3061 ataaatgcac ggactattac aagcctgaag ccgaacaggt tttaatatgg aacgacccg
//
LOCUS       DAWIVB010000198         3093 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWIVB010000198 DAWIVB010000000
VERSION     DAWIVB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.198"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(241..717)
                     /gene="greA"
                     /locus_tag="WIX60_001901"
     CDS             complement(241..717)
                     /gene="greA"
                     /locus_tag="WIX60_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ5306118.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VIYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1113..2414
                     /gene="aroA"
                     /locus_tag="WIX60_001902"
     CDS             1113..2414
                     /gene="aroA"
                     /locus_tag="WIX60_001902"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ5306119.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2457..2990)
                     /locus_tag="WIX60_001903"
     CDS             complement(2457..2990)
                     /locus_tag="WIX60_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ5306120.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          660 a          834 c          844 g          755 t
ORIGIN      
        1 tcctagattc ccgcttttgc gggaatgacg gcgacagggt tgctgttata gcggatgaac
       61 aaaaaccagt acggggttgt ctcgccttag ctcaaagaga acgattctct aaggtgctga
      121 agcaccaagt gaatcggttt cgtactatct gtactgtctt cggcttcgtc gccttgtcct
      181 gatttttatt aatccactat aatttcctgc gtgtgtcggg tgtatcgaaa tcaagccgaa
      241 tcaaatatat cggacttcga taatgtcgta ttcgcgcacg ccgcccgggg cttggacttc
      301 cgccgtatcc ccctcttcct tgccgattaa ggcgcgggcg atgggtgagc cgacatagat
      361 tttgccctgt ttgatgtcgg cttcgtcttc gccgacaatt tgatagataa cgtgttcttc
      421 cgtttccaaa tcttccagcg taaccgtcgt accgaacacg attttgcctt cggcgtggat
      481 ttcggtcgga ttgatgatgt gggcaacgga aagtttgtgt tccagctcgg aaatgcggcc
      541 ctcgataaag ccttggcgtt ctttggcggc ttcgtattcg gcgttttcgg acaaatcgcc
      601 gtgcgaacgg gcttcggcaa tcgcttcgat cacttcggga cgcgccacgc ttttgagctg
      661 ctgcaattcc tgtttcagca attccgcacc gcgtacggtc agggggattt tttgcatcgg
      721 tcttttttct ccatattccg gcacaccggt ttgcggcagc aagcataccg cgtaccgtct
      781 tgttttgtgc gtccggatat taaaataaaa atacaagccg cccggaaaat cggcggcttg
      841 tctgtcgttg aacagcggct attctaccaa attctatgaa attggcaatc gtgccgcgcc
      901 gccggcaaac gcgccatgtc cgcaacaaaa gctgaaaata tgccgacaaa gaaattttag
      961 aaacaaaaaa tttaaaaata atcaattttc ggcataaaaa accacattta cggactttaa
     1021 aaccgaaaat gccaagcctg agatttttca tacagcattt gcaccagtat aatgcaggct
     1081 gtttttatct ttaataatat tgacgttttg ccatgaccga atccgtccgc ctccccgtcg
     1141 cccgtctcaa accttccacc gtcgccctgc ccggctccaa aagcatcagc aaccgcaccc
     1201 tgctgcttgc cgccttgtcc gacaatgctt gcgaaatcca ttccctgctc aaatccgacg
     1261 ataccgaccg tatgctcgaa gcactcgata aactcggcgt tcaaatcgaa tatcttgccg
     1321 aagaccgtct gaaagtgcac ggcacaggcg gacgcttccc caaccgcact gccgatttgt
     1381 ttttgggcaa cgcgggcacg gcgttccgcc cgttaaccgc cgctctggcc gttttgggcg
     1441 gcgattatca tctgcacggc gtgcctcgta tgcacgaacg tcctatcggc gatttggtcg
     1501 atgcgttgcg gattgccggg gccgatgtcg aatatctcgg caaggaacac tatccgccgc
     1561 ttcatatcgg cgaacgccaa gacaacggcg agcgcgtgat tccgattaaa ggcaatgtgt
     1621 ccagccagtt tctgaccgcc cttttaatgg cgttgccgct gaccgggcag gcgtttgaaa
     1681 tccgtatggt cggcgaattg atttccaagc cctatatcga cattacttta aaactgatgg
     1741 cgcaattcgg cgtacaggtt atcaatgaag gctaccgcgt cttcaaaatt cccgccgatg
     1801 cgcactacca cgcgcccgaa cacttgcacg tcgaaggcga tgcctccagc gcgtcctact
     1861 tcctcgcagc cggtttgatt gccgccacgc ccgtccgcgt taccggtatc ggcgcaaaca
     1921 gcatacaggg cgatgtcgcc tttgcccgcg agctggaaaa aatcggggcg gacgtggttt
     1981 ggggcgaaaa cttcgtcgaa gtttcacgcc cgaaggaacg tgccgtccaa tcctttgatt
     2041 tggatgcgaa ccatatcccc gatgccgcca tgaccctcgc catcgtcgcg cttgctacag
     2101 ggcaaacctg cacgctgcgc aacatcggtt cgtggcgcgt caaagaaacc gaccgcatcg
     2161 ccgcaatggc aaacgagttg cgcaaactcg gggcaaaagt cgtcgaagaa gccgaagcaa
     2221 ttcacatcac cccgcccgaa acgctgacac ccgacgccgt catcgacacg tacgacgacc
     2281 accgcatggc gatgtgtttc tcgctggttt cgctgttggg cgtacccgtc gtcatcaacg
     2341 atccgaaatg cacccacaaa accttcccga cttatttcga cgtgttctca tcgctgaccg
     2401 aaacagcgga ataaggcggc attttgccgc gattccggcg cggcgcggcg ggcggctcat
     2461 tctgtaaaaa aagtatgtgc gccgaggtag tttttggcgt aaaacggtgt ggagagtttt
     2521 tcggttttga tggttttgcc gctgctgggg gcatggatga attcgccgtt gccgatgtag
     2581 agtccgacgt gtgagtagcg gtgtgcgccg ccggtgttga agaatacgag gtcgccggcc
     2641 ttgaggcggc tgtcggggat tttgcggctt gccgccgcca tgtcgcgggc ggtgcgcggc
     2701 agcttgacgt tgagggcgtt tttgtaaacg aattgaatca tgccgctgca atcgaagccg
     2761 gttgcggtgc tgctgccgcc ccatttgtag ggcgtgccga tgagtccgag gctgtggagc
     2821 atgagttcct gcgagccttg tgtgcggtcg atgtggctga tgcggacggc ttggatttgc
     2881 cggactgtct gtttgggttt cggttggcgg tgtttgccgg aggtcgtgcc gcatgaggcg
     2941 aggagcagtg cgctgagaca gaggaaaagg gttttgtcgg ggggaaacat ggtttttcct
     3001 ttgcgggttc ggatatccgt ctgaaggtgt ttcagacggt atagtggatt aacaaaaatc
     3061 aggacaaggc gacgaagccg cagacagtac aaa
//
LOCUS       DAWIVB010000199         3070 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWIVB010000199 DAWIVB010000000
VERSION     DAWIVB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.199"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(1..603)
                     /locus_tag="WIX60_001904"
                     /pseudo
     CDS             complement(1..603)
                     /locus_tag="WIX60_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            complement(808..1116)
                     /locus_tag="WIX60_001905"
     CDS             complement(808..1116)
                     /locus_tag="WIX60_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306121.1"
                     /translation="MDVFPELNIHCFYDDKNQLEAIEIFEPNKFFLEDISFNWLGKIN
                     DEIISYLYKSGINDFEIDDLGIRLLNIPVSTIINSIGKVECCYLDFSNRDTKFYVKQV
                     "
     gene            complement(1567..1890)
                     /locus_tag="WIX60_001906"
     CDS             complement(1567..1890)
                     /locus_tag="WIX60_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306122.1"
                     /translation="MSILEENVIDSASIENGILILTISDHLKWDNEHLFLLQEKINSY
                     IQYIESGQIFEDFGESSYETIEIQLIYKYKPNENYRKFLYRLEGALLKLKLRFSHGTI
                     SYFYS"
     gene            complement(1890..>3070)
                     /locus_tag="WIX60_001907"
     CDS             complement(1890..>3070)
                     /locus_tag="WIX60_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ5306123.1"
                     /translation="HGAAGGLTTGSLGGILAGGGTSLAAPYLDKAAENLGPAGKAAVN
                     ALGGAAIGYATGGSGGAVVGANVDWNNRQLHPKEMALADKYAEALKREVEKREGRKIS
                     SQEAAMRIRRQILRWVDKGSQDGYTDQSVISLIGMKGEDKALGYTWDYRDYGARNPQT
                     YNDPKLFEEYRRQDKPEYRNLTWLHSGTKDTKIRQGERKNEEFALNVAEGLTSLVNPN
                     PRIKVPILAGIRNLKNIKPTVTGSDPLLAGAGNIRIPANGNVAKGDRIPDTALASKGI
                     KHKNRKDQLEKNKKSGEDFEMEIYQKKVKQGFKPQRQITVKTKSGVKTRLDIISKEGG
                     LDVCTECKASITAPLTKNQKKAFPEIERTGATVVGKGKPGYPKGTKIEPTKVIIERKR
                     "
BASE COUNT          724 a          683 c          615 g         1048 t
ORIGIN      
        1 cggtaaccgt gccgtcccag ccgccgttca ggtcgaaact gccgcctatg ccgaagcttt
       61 tgccttcgta gcggctgtgg ttttgaatgt cgctatgggt gagggtggcc gtctgaaaaa
      121 ggtttttgcc cttatcttct gcgctttggc tagacgtgat gataccgccc ttgaggtctg
      181 tgttgtctct gactttgatt tgatagccgt cttctccggc ataaataccg ctttgctcgg
      241 ttaccgaagc atggtcggct cggattttgc tttggctgta atcgccactg gcactgaagc
      301 cataacctac ggtcacttgt gcactggcgt tttgttgttt gctttgatag gtttcagtat
      361 cttgaacact ttctatatgc aggttgcgcg tatctgcctg tatgcctttg ccgatgagct
      421 gcgcaccttt gagggtggta tccccgccgc ttcgaatggt agttttaccg gttgtgctgc
      481 cgacatgggt gtggcggtgg gttgcttttg tctgattgct gtggtggctt gttgaaagaa
      541 aggctgtctg aaacgtattt gttgtttcag acagcctcct ggctcaaacc ttgaaaacta
      601 catatgtgcg ttccgcacat cctacgtatt gagtttaggt ttcacatgag ctacggcttg
      661 ctatgccgtc ttttttccag gtgtggccgc ggctttggtt ttcggcaagg taggcgttta
      721 cttggtcaga tggcggattt tttgttcgtc gtagatgatg gagacgctga taccggagta
      781 agcgtagatt gggtcttgac ctcaaaccta cacttgtttt acataaaatt tcgtgtctct
      841 atttgaaaaa tctaaataac aacattctac tttacctatt gaattgatta tagttgaaac
      901 aggaatatta agaagcctaa tacccaaatc atcaatttca aaatcattaa ttccactctt
      961 ataaagatag cttattattt catcattaat ttttccaagc caattaaaag aaatatcttc
     1021 taaaaaaaac ttatttggtt caaatatctc tatcgcttca agctgatttt tatcatcata
     1081 aaaacaatgg atattcaatt cgggaaaaac atccatagga acccgagagt atgatgactt
     1141 ataaatttct tgtacatcag aactaaatat tgcacgaact tgttttcgat tcagatttga
     1201 tgagataata tttctttcta ttttttttga actaaaataa aaaattgcca taattttttt
     1261 gcctaaacaa tattaccatt ttcgtaagat gcatagaaca aaccatgtct tatccatttt
     1321 gttccatcgg cagacagata acgactatat ctaaatttta tttttcactc tcataaaaaa
     1381 ttttctgcaa tattcaatat atttactttc ttaaccatag cgtaaattcc tcaggcttat
     1441 atatttcagt ataagtatga cttaaaggat atgacgccgc gtgttacgag ttgcttcttt
     1501 tgatttcagg gtttatataa gttatggctt gcctgggctc gaagtgataa agagagtatt
     1561 tacttttcaa ctataaaaat atgagatagt tccatgggaa aaccgtaatt taagttttaa
     1621 taaagcacct tctaggcgat ataaaaattt tctataattt tcatttggtt tatatttata
     1681 tataagctgt atttcaatag tctcatagct actttctcca aaatcttcaa aaatttgacc
     1741 tgattcaata tattgaatat atgaatttat tttttcttgc aacaaaaaca aatgctcgtt
     1801 atcccatttt aagtgatcag aaatagttaa tatgagtatt ccgttttcaa tagaggctga
     1861 atcaattaca ttctcttcca aaatagacat tatcttttcc tttcaattat aactttagta
     1921 ggttcaattt tggtcccctt tggatagccc ggttttccct taccgaccac tgttgctccc
     1981 gttctttcaa tttcaggaaa agcttttttc tgatttttag taagtggcgc agttattgaa
     2041 gccttacact ctgtacaaac atcaagacca ccttctttcg aaataatatc aagcctagtt
     2101 tttacaccac tttttgtttt aactgtaatc tgtctttgcg gtttaaagcc ttgtttaact
     2161 ttcttctgat aaatttccat ctcaaaatcc tcaccagatt ttttattttt ttccagttga
     2221 tctttacgat ttttatgttt gattcccttg ctagccaatg ccgtatccgg aatcctgtcc
     2281 cccttcgcaa cattgccgtt tgcagggata cggatattcc ccgcacccgc caataaggga
     2341 tcgctgccgg taactgtcgg cttgatgttt ttcaggttgc ggatgcctgc aagaatcggg
     2401 acttttatcc tcggattggg gttgacaagg ctcgtcagtc cttcggcaac gttcagtgca
     2461 aactcttcgt ttttccgctc tccctgcctg attttggtgt ctttcgtccc gctgtgcagc
     2521 caggtcaggt tgcggtattc gggtttgtcc tgtcggcggt attcctcaaa cagcttcgga
     2581 tcgttgtagg tttgcggatt tcttgcgccg tagtcgcggt agtcccaagt ataacccaag
     2641 gctttgtctt cgcctttcat tccgataagg gatatgacgc tttggtcggt atagccgtct
     2701 tgggaacctt tgtccaccca acgcagtatc tgcctgcgga ttctcattgc cgcttcttgg
     2761 ctgctgattt ttctgccttc gcgtttttca acttcgcgct tgagggcttc ggcatatttg
     2821 tcggccaacg ccatttcttt cggatgcagc tgcctattgt tccaatctac attcgcaccc
     2881 accacagcac caccactacc accagttgca tagccgatgg ccgcaccgcc cagtgcgttg
     2941 accgccgctt tgcccgccgg accgaggttt tccgccgctt tgtccaaata cggtgcggca
     3001 agggaagtgc cgccgccggc cagtatgccg ccgaggctgc cggtcgtcag tccgcctgcc
     3061 gccccgtgca
//
LOCUS       DAWIVB010000200         3002 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWIVB010000200 DAWIVB010000000
VERSION     DAWIVB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.200"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(60..1430)
                     /gene="ffh"
                     /locus_tag="WIX60_001908"
     CDS             complement(60..1430)
                     /gene="ffh"
                     /locus_tag="WIX60_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ5306124.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFINNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLAGRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
     gene            1650..2456
                     /locus_tag="WIX60_001909"
     CDS             1650..2456
                     /locus_tag="WIX60_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ5306125.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGCGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            2912..>3002
                     /locus_tag="WIX60_001910"
     rRNA            2912..>3002
                     /locus_tag="WIX60_001910"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          684 a          748 c          758 g          812 t
ORIGIN      
        1 cagaatataa aacaaaaacg ccgtctgaaa tttcagacgg cgttttctgt taaatcggct
       61 tacaaacccg ggaacatccc ttttatcccc ctcattcctt tcgccatacg catcagtttg
      121 cccaagccgt tgccgctgaa catcttcatc atttgttgca tttgttcaaa ctgtttgagc
      181 aatttgttca cttcctgcac ggttgtgccc gcacccattg caatacggcg tttgcggctg
      241 gctttgagca gggcagggtt ggcgcgttct ttaggggtca tcgagttgat gatggcttct
      301 actttgccca tcgctttttc agccgttcct tcggggattt gtttcgagat ttgacccagt
      361 tcgcccggca ttttcgacat caggttttcc aaaccgccca tattgcgcat ttgctggatt
      421 tgttctttaa agtcgttgag gtcgaagcct ttgcctttgt gcagcttttt cgccatttta
      481 gcggcggctt cttcgtctat acctttttga acgtcttcaa tcagggtcaa tacgtcgccc
      541 atacccaaaa tgcggccggc aagacggtcg gggtggaaag gttcgaggcc gttgattttt
      601 tcgccgacac cgataaattt aatcggtttg ccggttacgt ggcgtacgga caatgccgca
      661 ccgccgcgcg agtcgccgtc catcttggtc aatacgactc cggtcagcgg cagggcttca
      721 ttaaatgcct gagcagtgtt caccgcatcc tgacccagca tcgcatcgat gacgaacaaa
      781 gtttccaccg ggttaaccgc cgcgtgaagg gctttgattt cgttcatcat ctcttcatcg
      841 attgccaaac ggccggcggt atcgaccatc aatacatcgt aaaaatgttt tttggcgtaa
      901 tcgacggcgg cagttgcaat ttcaaccggt ttttggttgg tatcggacgg gaaaaaatcc
      961 acgccgacct gttcggccaa cagacgcagc tgttcaatcg cggcaggacg gtaaacgtcg
     1021 gcggatacca ccaaaacctt tttcttctga tcgtttttca acaggcgggc gagtttgccg
     1081 acggtcgtcg tcttgcctgc gccctgcaaa cctgccatca acacgacggc gggcggcgca
     1141 accgacaaat ccagcgtttt gttttccctg cccatcagtt cggtcagggc tttgttgacc
     1201 acgccgataa atgcctgatc cggcgtcagg ctgcccgcta cttcctgacc gagggccttt
     1261 tctttgacgt tgttgatgaa ctctttgacg acaggcaggg cgacatccgc ctcaagcagg
     1321 gcgaggcgga cttcgcgcaa ggcctcttta atattgtctt cggtcagttt ggcctgcccc
     1381 cggatgtttt tgaagacatt gctgaagcgg ccggttaaat tgtctaacat actggtcctt
     1441 ggtctgaata agaatagctt gccccatcag gggcattctt tgttaaaata aaatcaaaat
     1501 aatttgatgc ggcttgtgtg ccggacagca tatcggcaaa tccgtcaagg cttgaccgaa
     1561 atggggattt tacaattcca acgttaaaag ttccaatatt tcataagcgg ccgcatacgg
     1621 cgcaacagta tagatagaga aagtccacca tgccgacagt tttcatcttt ttgacggcgg
     1681 tttacgcagg attgggtgca tttgcatggc actgccaaca gcaggggtgc ggccgggatt
     1741 acccgtggaa gacggaattg ccggttttgg gtgcggcatt gaccgtccac ggcgcggcac
     1801 tgcttatgcc ggtcattcaa gacaaaatca tcattatggg cttcgggtat tccggcagcc
     1861 tgattgtttg gatgatgctg tttatttatt ttgccggcag cttcttttat ccgctgcgcg
     1921 gagtgcagtt gctgctgtat ccttgcgccg cactgatgct gctgtcaggt ttggtttttc
     1981 ctggaaaatt ctcgggatat gaaattaccg accttccctt tatgctgcat atcggaactt
     2041 cgctgctcgc atacgggctg ttcggcatcg caacattatt gtccgttttg accctgctgc
     2101 tgaatcggag cctgcaccgc aggagcttct ccaagctcgc aggattcctg ccgtcgctgc
     2161 tcagtttgga aaaactcatg ttccaggcca tgtgggcagg tttcatcctg ctgacctatt
     2221 ccgtcgtcag tggaacattt tttgccgaag ccgtattcgg caaacccatg acctttaccc
     2281 ataaaaccgt attcggcata ttgtcatggc tgatttacgg cggactgctg ctcaagcaca
     2341 gcatgaccgc atggcgcggc aaaaaagccg ccgtgtggac catcatcgga tttgtcagcc
     2401 ttatgattgc ctatatgggc agcaagttcg tattggaaat cattctgaaa agataagaag
     2461 agccaacaga tgccgtctga gtccccgagt ttcagacggc atattcacaa aggcgcacca
     2521 gccggaggag ggagaggaaa ggattgttgg aggcggcgca gtatttagca gaaataaaaa
     2581 accttatccg acagcgacat gacgaatttc cccaaaaaaa tcccgctgaa agcattgacc
     2641 gtttttccct gtgggcgtat agttcggttc ttcgctgctg cagaagcggc ggacgaactg
     2701 aaaagtatag cacagaatgt tggggatatc gagagatatc ttgacaggcg gaaggaatat
     2761 ttataattcg caacgctctt taacaaaaca gattaccgat aagtgtgagt gccttgagtc
     2821 tcacactgtt tgaaagacag acaagataat gttttgaaca ttgtcctgtt ggtttctttg
     2881 aagcagacca gaagttaaaa agttagagat tgaacataag agtttgatcc tggctcagat
     2941 tgaacgctgg cggcatgctt tacacatgca agtcggacgg cagcacagag aagcttgctt
     3001 ct
//
LOCUS       DAWIVB010000201         2969 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWIVB010000201 DAWIVB010000000
VERSION     DAWIVB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.201"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            124..1668
                     /gene="purF"
                     /locus_tag="WIX60_001911"
     CDS             124..1668
                     /gene="purF"
                     /locus_tag="WIX60_001911"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ5306126.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1768..2259
                     /gene="greB"
                     /locus_tag="WIX60_001912"
     CDS             1768..2259
                     /gene="greB"
                     /locus_tag="WIX60_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ5306127.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRDDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2318..2944
                     /locus_tag="WIX60_001913"
     CDS             2318..2944
                     /locus_tag="WIX60_001913"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ5306128.1"
                     /translation="MRKIRTKICGITTPEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIRNAATRFPDAQALLFDAYHPSEYGGTGNRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAESVDVSGGVEASKGKKDAAKVAAFIATANRLSR"
BASE COUNT          713 a          905 c          776 g          575 t
ORIGIN      
        1 tcttattctc cataaaccca cctatctgtc cttcgctcaa atattgtcaa caattcaggc
       61 aaaaaaccac cgtttacttt ataattccgt tttaccctta aacctcgaat cggagaagac
      121 gatatgtgcg gcgtattagg tttggtcagt catgaacccg tgaaccagct tctgtacgac
      181 ggcttgcaga tgttgcagca cagggggcag gatgcggcgg gcattgcaac ggcggaaggc
      241 ggtaccttcc atatgcacaa aggcaaaggc atggtgcgcg aagtgttccg cacacgcaat
      301 atgcgcgatt tgaccggcaa cgccggcatc gcccacgtcc gttatcctac tgcgggcaac
      361 gcaggcagca gcgcggaggc gcagcctttc tacgtcagct cgccgttcgg catcgttttg
      421 gcgcacaacg gcaacctcac caacactgcc gaactgtatg aaaacgtgtg taacaaacac
      481 ctgcgccacg tcaacaccag ctccgattcc gaagtcttgc tcaacgtatt cgcgcacgaa
      541 ttacgccgcg aagtctctaa aaacgccgac ccgcaccggc tcaatgccga caatattttc
      601 aacgccgttg cccaagtcca ccgcctggtg cgcggcgcat acggtgtgat tgccatgatt
      661 gcgggctacg gtatgctcgc cttccgcgac ccttacggca tccgcccgct ggtattgggt
      721 tcgcaaaccg acagcgaagg cagaaaatcc tatgccgtcg cctccgaatc cgtcgccttc
      781 aacgcgctta cctacgattt ggaacgcgac atccggccgg gcgaagcggt atttgtcggc
      841 tttgacggca caatgattgc ccgccaatgc agcgaccgtg ccaaactcag cccctgcctt
      901 tttgaatatg tttattttgc ccgccccgac tctgtgattg acggcgtatc cgtttaccaa
      961 tcgcgcttgg atatgggcgt gtcgctggcg gaaaaaatca aacgcgagct gcccgtggac
     1021 ggcatcgatg tcgtgatgcc cattcccgac accagccgcc ccagcgcgat ggagcttgcc
     1081 gtccatctcg acaaacccta ccgcgagggt ttgattaaaa accgctatat cggccgcacc
     1141 tttattatgc ccggacaggc gacgcgcaaa aaatccgtgc gccaaaaact cagcccgatg
     1201 gaaaccgaat ttgcaggcaa aagcgtgttg ctggtggacg actccatcgt gcgcgggacg
     1261 accagccgcg aaatcgtcga aatggtacgc gcggcgggcg cgcgcaaagt ctatatcgcc
     1321 tccgccgcgc ccgaagtgcg ctatcccaat gtgtacggca ttgatatgcc cacgcgcgaa
     1381 gagttgattg ccaacgggcg cagcgcggcg gaaatcgccg ccgaaatcgg cgcggacggc
     1441 atcgtatttc aagacttggg cgatttggaa gccgtcgtca aagcactcaa tccgaaaatc
     1501 gaatccttcg attcgtcctg tttcaacggc atttatcaga ccggcgacat cgatgatgcc
     1561 tacctcgacc gcctgtctgc cgagaaatcc ggctgcggcg gtctgaaaat ccacccgagc
     1621 aggatggaac acagcatcag catcagcgat gcgggcgacg aagaataagg cttcccaaac
     1681 cgcgatgccg tctgaaatcc gaacgccggg ttttcagacg gcatttccca tatcggcatc
     1741 acatttcccg tttcatccgc acacaatatg agtaccgaaa ccaaaaacta cattacgccc
     1801 gccggatggc aggcattgaa agacgagctt tatcaactgg tcaacaaaga acgccccgaa
     1861 atcgtccaaa tcgtcaactg ggcggcaggc aacggcgacc gcagcgaaaa cggcgactat
     1921 ctttacggca aacgccgtat gcgcgaaatc gaccgccgca tccgtttcct gaccaaacgt
     1981 ttggaggctg ccgtcgtcgt cgatcccgaa ttgcgcgaag cgaccgacca agtgtttttc
     2041 ggcgcgacgg tcggactgtt gcgcgacgac gggcgcgagc aaaccgtcaa aatcgtcggc
     2101 atcgatgaaa tcgataccgc gcaaaacaaa atttcttgga tttcgccgct ggctcgttgt
     2161 ctgattaaag cgcgcgaagg cgacgaagtg gttttgaaca cgccggaagg gcgtgaggaa
     2221 attgaaatcc tttctgtcga atacatcaaa atcgactgat cgggaatgcc gtctgaaacg
     2281 gcaaaagcgc gccgtttccc aaataaagga ataccgaatg aggaaaatcc gcaccaaaat
     2341 ctgcggcatc accacaccgg aagacgcagc tgccgccgca gcggcaggtg cggatgccgt
     2401 cgggctggtc tttttccaag gcagcagccg ggccgtcgat attgcccgcg ccaaaaaaat
     2461 caccgccgca ctgccgccgt ttgtcagcgt tgtcgccctt ttcgtcaacg aaagcgcgca
     2521 aaacatccgc cgcatccttg ccgaagtgcc gatacacatc atccaattcc acggcgacga
     2581 agacgacgca ttctgccgcc agttccaccg cccctatatc aaagccattc gtgttcagac
     2641 ggcatcagac atccgaaacg ccgccacgcg cttccccgac gctcaggcac tgctgttcga
     2701 tgcctaccat ccttcggaat acggcggcac cggaaaccgc ttcgactgga cgctgctggc
     2761 ggaatattcg ggcaaaccgt gggtgcttgc cggcgggctg acccctgaaa acgtcggcga
     2821 agccgtccgc atcaccggag cggaatcggt cgatgtatcc ggcggtgtgg aagcgtctaa
     2881 aggcaaaaaa gatgccgcca aagtcgccgc ctttatcgca accgccaacc gcctatcccg
     2941 ttaaagcaac aaaaattgcc gccggaatg
//
LOCUS       DAWIVB010000202         2948 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWIVB010000202 DAWIVB010000000
VERSION     DAWIVB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.202"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(369..953)
                     /gene="rnhB"
                     /locus_tag="WIX60_001914"
     CDS             complement(369..953)
                     /gene="rnhB"
                     /locus_tag="WIX60_001914"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ5306129.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVEWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1022..2917)
                     /gene="mnmG"
                     /locus_tag="WIX60_001915"
     CDS             complement(1022..2917)
                     /gene="mnmG"
                     /locus_tag="WIX60_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ5306130.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLSHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNADMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVSEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEGEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT          497 a          720 c          939 g          792 t
ORIGIN      
        1 aaggcgggaa tctaggtctg tcggtacgga aacttatcgg gtaaaacggt tttgccagcc
       61 ctgagactcc ggattcctgt tttcgtagga atccggtttt ttgagcttca gtcatttttg
      121 ataaattctt gcagctttga gtttctagat tcccgctttc gcgggaatga cggtttggaa
      181 gttacctgaa attcaaaaaa aaaacggaaa ccggacggat tggattcccg cctgcgcggg
      241 aatgacggat tttaggtttt tttttgattt tctatttttc gcgggaatga cggtttgggt
      301 tctttctctt tggagttgcg atgccggaaa tgccgtctga aggcttcaga cggcattttt
      361 gtgccggttt aaaacaaggc ctgctgcgcg agcaggtttc tgacgggggc gaagtcgcgg
      421 cggtgttcgg gcagcacgcc gtatttttcg agggcttcca aatgctgctt cgtgccgtaa
      481 cctttgtgtt tgtcgaaacc gtattgggga tggcgttgcg ccagtgcgta catttccgca
      541 tcgcgtgcgg tctttgccaa aacggatgcg gcggagattt cgatgatttt gctgtcgcct
      601 ttgacgacgg cttcggcagg gatgttcaaa tgttcaggaa tgcggttgcc gtcgatgaat
      661 attttttcgg gacgcacagc caagccgtca acggcgcgtt tcatcgcgag catggtggcg
      721 tgcaggatgt tgaggctggc gatttcttcg ggcgaggcgg cggcaacgtg ccactcaacc
      781 gcctgatttt ttatcatttc ggcaagcgcg tcgcgttttt tctcgctgag ttttttggag
      841 tcggtcagtc cgggcaggtc gaatgtttcc ggaaggatga cggcggcggc aaacacgctg
      901 ccgactaaag gtccgcgtcc tgcctcgtcc acgccggcgg tcagtatgtg catgatgttt
      961 cctgtcggga tggtgggaat gccgtctgaa aagggtttca gacggcatcg cgccgatgtg
     1021 tttatttcgc gtctttaaac ccgcgcttca aatgcaccat cagcaatgcc actgccgcag
     1081 gggttacgcc ggaaatgcgg ctggcttgtc cgacggtttc gggtttgtgc tggttgagct
     1141 tttgctgcac ttctgccgac aagcctttga ctttgccgta atcgatgccg tcgggcagtt
     1201 ttaaggtttc gatgtcgcgg cggctgtcga tttcttcgtt ttggcggtcg atatagcctt
     1261 ggtatttgac ttggatttcg acttgttcga tgacttcggc ggagaggttt tcagacggca
     1321 tcgcgccttc gagcgtcatc agcgcggcgt agtcgaggtt tgggcggcgc aggaggtcgt
     1381 gcaggttggc ttcgcggctg agtttttgtc cgaacacacg gatttgttcg ccttcggcga
     1441 gtttttgcgg cgtgtaccac gttgttttca aacgttggat ttcgcgttcg acggcttcgc
     1501 gtttttcgtt gaacatgcgc cattgcgctt cggacaccaa gccgattttg tagccgtctt
     1561 cggtcaggcg catgtcggcg ttgtcttccc tgagttgcag gcggtattcg gcgcggctgg
     1621 tgaacattcg gtagggttcg ttcacgcctt tggtgatgag gtcgtccacc aatacgccga
     1681 ggtaggcttg ttcgcggcgc agcaggagcg ggtcttgtcc gcgcacatat tgcacggcgt
     1741 tcgcgcctgc caataaacct tgcgcggcgg cttcttcgta gccggtcgta ccgttgattt
     1801 gcccggcgaa aaacaatccg gcaatggttt tggtttcgag gcttgctttg aggttgcgcg
     1861 gatcgaagta gtcgtattcg atggcgtagc cggggcgcag gatatgggcg ttttccaaac
     1921 ctttcatact gcggacgagc gcgatttgga tgtcgaacgg caggctggtg gagataccgt
     1981 taggatagta ttcgtgcgtg gtcagacctt cgggttcgag gaaaatctgg tggctgtctt
     2041 tgtcggcgaa gcggttgatt ttgtcttcga tagacggaca ataacgcgga cccacgcctt
     2101 cgattttgcc ggtaaacatc gggctgcggt cgaagcctga gcggatgatg tcgtgggttt
     2161 gcgtgttggt atgcgtaatc cagcaggaca cttggcgcgg gtgcatatcg gcgttgccgc
     2221 gcacggacat gacgggaacg ggcgtgtcgc cgggctgttc ggtcagttgg gagaagtcaa
     2281 tcgtgcgtcc gtcaatacgc ggcggcgtgc cggttttcag acggccttgc ggcagcttca
     2341 attcgcgcaa acgtccgccc aacgatttgg cggcggggtc gccggcgcgt ccgccttcgt
     2401 agttttccaa accgatgtgg attttgccgg acaaaaacgt gcctgcggtc aacacgacgg
     2461 cgcgtgcttt aaactccacg cccatcgcgg taattacgcc gctgatgcgt tcgccgtcga
     2521 gcgttacgtc ttcgacggct tgttggaaaa ggtcgaggtt ttcttggttt tccaacattt
     2581 cgcggatggc ggctttgtac aggatgcggt ccgcctgcgc gcgcgtggca cgcactgccg
     2641 cgcctttgct ggcgttcagg cggcggaact ggataccgga tttgtcggtt gccaacgcca
     2701 tcgcgccgcc gagcgcgtcg agttcgcgca ccaaatgccc tttgccgatg ccgccgatag
     2761 aggggttgca cgacatttgt ccgagcgttt cgatattgtg tgagagcaaa agcgtctgcg
     2821 cgcccatacg ggcggcggcg agtgcggctt ccgtgccggc gtgtccgccg ccgacgacga
     2881 taacgtcgta ggttttgggg taaatcatgt gggtcatagt gtgtattgcc tgacggtgtt
     2941 tcagacgg
//
LOCUS       DAWIVB010000203         2893 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWIVB010000203 DAWIVB010000000
VERSION     DAWIVB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.203"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(125..1117)
                     /gene="pdxA"
                     /locus_tag="WIX60_001916"
     CDS             complement(125..1117)
                     /gene="pdxA"
                     /locus_tag="WIX60_001916"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ5306131.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGASEEGVLEVLHIPAAEAVEAGKLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLNKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1277..2704
                     /locus_tag="WIX60_001917"
     CDS             1277..2704
                     /locus_tag="WIX60_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ5306132.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          575 a          789 c          828 g          701 t
ORIGIN      
        1 ccaatggtct ggattcccgc ctgcgcggga atgacgattt ggaaattacc cgaaacccaa
       61 aaacaactga aaccgaacag accggattcc cgcctgcgcg gaaatgacgg gtcttttatc
      121 atctttaaag gctgccgcgc gccatctcga cggcggtctc cacggcagtt atcaggctgc
      181 cggaatccgc cctgccggtt gccgccaaat caagcgcggt gccgtgatcg acggaggtgc
      241 ggataaaggg caggccgagc gtgatgttca cgccctgtcc gaagctgtgg tatttcaaca
      301 cgggcagccc ttggtcgtgg tacatcgcca atacggcatc cgcaccttcg agcataaacg
      361 gttggaacaa ggtatccgcc ggatacgggc cggcgaggtt tatcccttcg cggcgcaggt
      421 tttcgagcgc ggggataatg gtgtcggttt cttcgtgtcc gaggtgtccg ccttcgccgg
      481 cgtgggggtt gagtccggcg actaggattt tgggattttt gatgccgaat ttgtgtttta
      541 agtcgtgatg caaaatgcgt gcgacgcttt caatcagcgg ttgcgtgatg gcggcggcaa
      601 cgtctttcag cggcaggtgg gtcgttacga gggcgacgcg caggcctttg ccggcaagca
      661 tcatcacaac ctgccccgtg ccgctttttt ccgccagata ttcggtgtgt ccgctgaaaa
      721 aacctgtgct tgcgcgcgcg tcgttgatga tgcctttgtt cagcggcgcg gtaacgatgc
      781 cgtcgaaaat gccgtctgaa atgcctgcga gcgcggtgtc tagaagttgc agcacatagg
      841 cggcgttggc gggattgagt ttgcccgcct caaccgcttc ggcggcaggg atgtgcagca
      901 cttccagcac gccttcttcg gacgcgccgc ctgattccgg atcgaagtcg cgcaggacga
      961 cgcttttgcc caaggcttcg gcgcgcgcgc gcaacaggtt tttgtcgccc aataccgcgc
     1021 agcggcaggg caggcgtgca aatgccaagt ccaaacaaat atcggggccg atgccggcag
     1081 gctcgccgga agtaacggca aaaacaggct gtttcatcgt gtttgctcca aacaaaatgc
     1141 gattctaacg ccgcagccgc gcggcgatgt aaatttttct gattttgttg acaatctgct
     1201 agaatgggcg tttacaaaat ttaaaccctg cttgcatacc gccaatatgt gcgagtttca
     1261 actttaagga agcgatatga acgagaactt taccgaatgg ctgcacggct gggtcggcgc
     1321 catcaacgat ccgatgtggt catacttggt ttatatgctt ttgggtacgg ggcttttctt
     1381 caccgtaacc acgggctttg tccaattccg cctgttcggg cgcagcatca aagaaatgct
     1441 cggcggccgc aaacaggggg acgaccctca cggcatcacg ccgtttcagg catttgtaac
     1501 cggccttgcc agccgcgtgg gcgtgggcaa tatcgcgggc gtggccatcg ccatcaaagt
     1561 cggcggaccg ggcgcggtgt tttggatgtg ggtaaccgcc ttaatcggta tgagttcggc
     1621 gtttgtcgaa tcttcgctgg cgcagctctt taaagtccgc gactacgaca accaccattt
     1681 ccggggcggc cctgcctact acatcactca agggctgggg cagaaatggc tgggcgtgtt
     1741 gttcgccctg agcctgattt tctgtttcgg ctttgtgttt gaagcggttc agaccaatac
     1801 catcgccgat accgtcaaag cggcatgggg ttgggagcct cattatgtcg gcgtcgccct
     1861 ggtgatttta accgcgccga ttatcttcgg cggcatcagg cgcatatcta aagcggcgga
     1921 aatcgtcgtc cccctgatgg cggttttgta cctctttatc gcgcttttca tcattttgac
     1981 caatattccg atgattccgg acgtgttcgg tcagattttt tcgggcgcgt tcaaattcga
     2041 cgcggcagca ggcggcttac tcggcggtct gatttcgcaa acgatgatga tgggcatcaa
     2101 acgcggcctg tattccaacg aggcgggtat gggttccgcg ccgaacgccg ccgccgccgc
     2161 cgaagtgaaa caccctgttt cgcaaggtat gattcaaatg ctgggcgtgt ttgtcgatac
     2221 catcatcgtt tgttcttgca ccgccttcat catcttgatt taccaacagc cttacggcga
     2281 tttgagcggt gcggcgctga cgcaggcggc gattgtcagc caagtggggc aatggggcgc
     2341 gggcttcctc gccgtcatcc tgtttatgtt tgccttttcc accgttatcg gcaactatgc
     2401 ctatgccgag tccaacgtcc aattcatcaa aagccattgg ctgattaccg ccgttttccg
     2461 tatgctggtt ttggcgtggg tctatttcgg cgcggttgcc aatgtgcctt tggtctggga
     2521 tatggcggat atggcgatgg gcattatggc gtggatcaac cttgtcgcca tcctgctgct
     2581 ctcgcccttg gcgtttatgc tgctgcgcga ttacaccgcc aagctgaaaa tgggcaaaga
     2641 ccccgagttc aaactttccg aacatccggg cctgaaacgc cgtatcaaat ccgacgtttg
     2701 gtaaatcccg cccttaccgg agccgcttcc ccgcgaagcg gcttttccct ttccgcacac
     2761 tgtaaaaaca gggcgaacaa gcgtacaatc ccaacccttt acttttgaat ccatttcgtt
     2821 tttcagacgg catattgaat atagtggttt aacaaaaatc aggacaaggc gacgaagccg
     2881 cagacagtac aag
//
LOCUS       DAWIVB010000204         2830 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWIVB010000204 DAWIVB010000000
VERSION     DAWIVB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.204"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            32..463
                     /locus_tag="WIX60_001918"
     CDS             32..463
                     /locus_tag="WIX60_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ5306133.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            474..1340
                     /locus_tag="WIX60_001919"
     CDS             474..1340
                     /locus_tag="WIX60_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ5306134.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAV
                     IAGEVWQALLYALVVLLMWLVGAVRRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCVG
                     TAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(1391..2443)
                     /gene="sohB"
                     /locus_tag="WIX60_001920"
     CDS             complement(1391..2443)
                     /gene="sohB"
                     /locus_tag="WIX60_001920"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ5306135.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIASAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2547..2636)
                     /locus_tag="WIX60_001921"
     tRNA            complement(2547..2636)
                     /locus_tag="WIX60_001921"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2600..2602),aa:Leu,seq:taa)
     gene            complement(2739..2812)
                     /locus_tag="WIX60_001922"
     tRNA            complement(2739..2812)
                     /locus_tag="WIX60_001922"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2778..2780),aa:Cys,seq:gca)
BASE COUNT          681 a          764 c          675 g          710 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaggcgaaca ggaccggcgc aaacggttgc tgtcgttgac
      301 cgaaacaggc aaagcctatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
      361 agtatttgcc acattcggcg acaagcgcac aactcggctg tttgccgatt tggatgcact
      421 ggctgaagtg atggaaaaaa caatctcgga aaataaaaaa taggggggca aatatgtgga
      481 aaatgttgaa acacatagcc caaacccacc gcaagcgatt gattggcaca ttttccctgg
      541 tcggactgga aaaccttttg atgctggtgt atccggtgtt tggcggccgg gcgatcaatg
      601 ccgtgattgc gggggaggtg tggcaggcgt tgctgtacgc tttggttgtg cttttgatgt
      661 ggctggtcgg tgcggtgcgg cggattgccg atacgcgcac gtttacgcgg atttataccg
      721 aaatcgccgt gccggtcgtg ttggaacagc ggcagcgaca agtcccgcat tcggcggtaa
      781 ctgcgcgggt tgccctgtcg cgtgagtttg tcagcttttt tgaagaacac ctgccgattg
      841 ccgcgacatc cgtcgtatcc atattcggcg cgtgcatcat gctgctggtg ctggaatttt
      901 gggtcggcgt gtcggcggtg ggcatacttg cgttgttttt atggcttttg ccacgttttg
      961 ccgccatcag cgaaaacctg tatttccgcc tgaacaacag cttggaacgc gacaaccact
     1021 ttatccgaaa aggcgaccgg cggcagctgt accgccatta cggactgctt gcgcgcctgc
     1081 gtgtgctgat ttccaaccgc gaagccttcg gctatctctg cgtcggcacg gcgatgggta
     1141 ttttgttcgg ctttgctttt gtgatgatga cgctcaaagg ctacagcagc gcggggcatg
     1201 tctattcggt cggcacttat ctgtggatgt ttgccatgag tttggacgac gtgccgcgat
     1261 tggtcgaaca atattccaat ttgaaagaca tcggacaacg gatagagtgg tcggaacgga
     1321 acatcaaagc cggaacttga aaaatgccgt ctgaacacgc ttcagacggc atttccatcc
     1381 gttcggcaaa ctacatcaca tccgcccgcc ggttgacaag tttggcaaac aacttttcaa
     1441 cagaagcttc cgcctgcaaa ccaatgcgct ggatcaggct ttgcttctcc tgatatttca
     1501 cttcgataac ctgtttgttt tcaaacgctt tcaacaacaa atcatcactg gtcgaaatct
     1561 cgtcaatcaa gttcaacgcc aacgcctgcc gaccgaacca atgctcgccc gttgccactt
     1621 cctcaatatc caattgaggg cggttctcgc tgacaaactg cttgaacaac tgatgcgttt
     1681 cctccagttc ctgtcggaat ttctgtttgc ccttttccgt attttcaccc ataaaagtaa
     1741 ccgtgcgctt aaattcgccc gccgtcatca catccacatc aatatcatgt tttttcaaca
     1801 ggcggtggat attcggtact tccgccacca cacccaccga accgacaatc gcaaacggag
     1861 cggaagcaat tttatccgcc acacacgcca tcatataacc gccgctcgcc gccaccttat
     1921 cgacggcgac ggtcagcgga atattgcgtt cgcgcaaacg cctaagctgc gaagccgcca
     1981 aaccgtaacc gtgaaccacg ccgcccggac tttccaatct gagcagaacc tcatcttcag
     2041 gcttggcaat caaaagcacc gccgtaatct catgacgcaa ggattctacg gcgtgtgcat
     2101 acaaatcgcc gtcaaaatcc aacacaaaaa ggcgggattt ttgcgtttcg gcagatttct
     2161 ccccaccctc cttcaaacgc tttttctctg ctttggcttc cgccttttcc tttttctttt
     2221 cctctttttc ctgatgtttt gcctcttccc cgcttaaaaa gaatgcttca aacgattgcc
     2281 gctgtttttt ataattttcc gaaaaatccg tcagtacgac actgccgctt tccgactgtt
     2341 tcttactctg tacgatagcc aacacaatca gcgcaattgc gccgaacacg gtaagcagtt
     2401 cgagcaggaa aataccgtaa ttcagtaaaa tttctttcca cattgattgg atttcctctt
     2461 gttcaggcat gaacatgtca atattgtcca tcaccgtccg acagataaaa aaataaccgc
     2521 ttggagcggc attgtcattt tcagcttggt gcccggagcc ggaatcgaac cggcacggga
     2581 tgtttagtcc cgacggattt taagtccgtt gtgtctacct atttcaccac ccgggcattt
     2641 gtgaaaggtg gaggcggggg cgcggatttt aaccggcctg tatgaagatt gcactcctca
     2701 tagcataaac actctgccac cccgccatag tacgataatg gaggcgagag tcggaatcga
     2761 accggcgtag acggatttgc aatccgctgc ataaccactt tgctatctcg ccctaaaact
     2821 ggcttatcta
//
LOCUS       DAWIVB010000205         2612 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWIVB010000205 DAWIVB010000000
VERSION     DAWIVB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.205"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            162..602
                     /locus_tag="WIX60_001923"
     CDS             162..602
                     /locus_tag="WIX60_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ5306136.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            654..1529
                     /gene="xerD"
                     /locus_tag="WIX60_001924"
     CDS             654..1529
                     /gene="xerD"
                     /locus_tag="WIX60_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ5306137.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            1701..1901
                     /locus_tag="WIX60_001925"
     CDS             1701..1901
                     /locus_tag="WIX60_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ5306138.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            2067..2300
                     /locus_tag="WIX60_001926"
     CDS             2067..2300
                     /locus_tag="WIX60_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ5306139.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
BASE COUNT          647 a          521 c          741 g          703 t
ORIGIN      
        1 gtggtttgac atggatattt ttcatgccgt cgtgtgtcgg tttggatgtt tccggcggtt
       61 gaatccttgt cctttggggc ggtagaatcg gggttggttt ggcaattgcg gcggtgcgtc
      121 tgcgtgccgt tttgaataat gggaatatcg ggagtaggac tatggatgtg aaatatgaat
      181 ttaccctgcc ttcgagcagc ggtgcggatt ttcattcggc agaacatctg cctttggtcg
      241 tgtattttta tccgaaagac agtacgcctg gctgtacgac ggaaggcttg gatttcaatg
      301 cgcgtttgga acagtttgag gcattgggtt ataccgtggt cggtatttcc cgcgacggcg
      361 taaaggcgca tcagaatttt tgcgccaagc agggtttccg gttcgagctg ttgagcgaca
      421 aggatgaaac agtgtgccgc ctgtttgatg tcatcaaatt gaagaaactg tacgggaaag
      481 agtcgttagg tatcgagcgc agtacgttcg tcttgaataa ggatggagaa atcgcccatg
      541 aatggcggaa agtcaaagtg gcgggtcacg cgcaggaagt attggaaacg ctttcccgat
      601 aatgtgaacc atgccgtctg aagaagattc agacggcatt tgtttggaac ggtatggaag
      661 aaggtttgat cgacaggctg cttgaaacgc tgtggttgga caggcggctc agtcagaata
      721 ctttaaacgg ttaccggcgc gatttggaaa aaatcgcccg ccgcctgtcc caatcgggca
      781 gaatgctgaa ggatgcggac gaagcggatt tggcggcggc ggtttatgtt gacggagagc
      841 aacggagttc gcaggcgcgc gcattatcgg cgtgcaaacg cctgtatata tggatggagc
      901 gtgaaggcat aaggacggac aatcccaccc gtttgctgaa accgcccaaa atcgacaaga
      961 atattccgac actgattaca gaacagcaga tttcccggct gcttgccact ccggatactg
     1021 atacgccaca cggtttgcgg gacaaggctt tgctcgaatt gatgtacgcg accggcttgc
     1081 gcgtcagcga ggcggtcggg ctgaacttcg gcaatgtgga tttggacagg ggctgtatta
     1141 ccgcgctggg caagggcgat aagcagagga tggtcccgat ggggcaggag tcggcgtatt
     1201 gggtggaacg ctattatacg gaggcacgcc cacttctgct gaaaggcagg aattgcgacg
     1261 cattgtttgt cagtcagaaa aagacgggca tttcccgtca gttggcatgg atgattgtca
     1321 aagaatatgc aagtcaggca ggcatcgggc acatcagccc gcacagcctg cgccacgcct
     1381 ttgccacgca tctggtgcgg cacggcttgg atttgcgcgt ggttcaggat atgttgggac
     1441 atgccgattt gaatacgacg cagatttata cccatgttgc caacgtatgg ttgcagggtg
     1501 tagtgaagga acaccattcc cgaaactgag gcttctactt tggtttaata agaaatcaat
     1561 cgaaaatgca aacttgataa aaaattacca agacaaaccg tgtataccga ccttgcaatg
     1621 cgaaccgttt ttatttatat tcgcatacga taataaaagc cgctatcggt acgatagttt
     1681 gagaacacac ggagcacaaa atgtttgtct gcatctgcaa tgccgttacc gaccatcaaa
     1741 tcaaggaaac catcgccgcc ggcgcgacca caatgggcga tttgcagtcg caattgggcg
     1801 tagcgagctg ctgcggctgc tgtggggagc ttgccgcttc gtttctgacg gcgcacaatg
     1861 cgcaaccgac ggttacggcg ggtatcaatg ttcaagcgta aaacggtttt ggaaatgccg
     1921 tctgaactgt tcagacggca tttttgctgt ttttggcagg acttgagtat catcttcctt
     1981 gaaaacattg ttttttccca aatagaccat gattctgctg cgtctaaggc tttggcgtgt
     2041 gcaaattgac agataaggaa acgcggatga aattgacctt gatgtttcgt gaatattgca
     2101 gcttgtgcca caaaatgcgc gatgcactca aaccttttca aaatgaatac gggttcgggc
     2161 tggaagtggt cgatgtggat gaaaatcctg ttttggaaga aaaatacaat gagctggttc
     2221 ccgttttgtt ggcgggagat gaggaaatct gccactggtt tttggatgag gacaggttga
     2281 aacagtttct cgaacggtaa aaaaatgccg tctgaagcag gacttcagac ggcatttttt
     2341 tcaaattaac gttctttacg tttttgcggg gctgatgacc tgccggtaaa ggaagcacgt
     2401 ttggatgctt ggtaaattgc tagtcttctt cgatgttgca tattaaccct ttctttattt
     2461 tatttgtcgg ttgggagaat tcttatttat tgattttttc aataaaaatt agaaaattta
     2521 ttgtgagatg ttattgttgg caatcatatc atgttttact gttgatggaa gcatgattgt
     2581 gtaaagatga tatgtgtttg tgtaatcggt ag
//
LOCUS       DAWIVB010000206         2580 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWIVB010000206 DAWIVB010000000
VERSION     DAWIVB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.206"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(195..266)
                     /locus_tag="WIX60_001927"
     tRNA            complement(195..266)
                     /locus_tag="WIX60_001927"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Arg,seq:cct)
     gene            complement(286..733)
                     /locus_tag="WIX60_001928"
                     /pseudo
     CDS             complement(286..733)
                     /locus_tag="WIX60_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
     gene            complement(738..1712)
                     /gene="ispB"
                     /locus_tag="WIX60_001929"
     CDS             complement(738..1712)
                     /gene="ispB"
                     /locus_tag="WIX60_001929"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ5306140.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1728..1916)
                     /locus_tag="WIX60_001930"
     CDS             complement(1728..1916)
                     /locus_tag="WIX60_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306141.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1936..2244
                     /gene="rplU"
                     /locus_tag="WIX60_001931"
     CDS             1936..2244
                     /gene="rplU"
                     /locus_tag="WIX60_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ5306142.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2269..2541
                     /gene="rpmA"
                     /locus_tag="WIX60_001932"
     CDS             2269..2541
                     /gene="rpmA"
                     /locus_tag="WIX60_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ5306143.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          756 a          693 c          575 g          556 t
ORIGIN      
        1 ataaaacaac agatttccaa gccggaacat cttttacgaa gcctgaaaat catttcatga
       61 ttctaccgtc ctaaaggtcg gtttttcaag caggaagaaa aagttttcag atggcaaaaa
      121 agccctccaa cactgaaagg ccttatatcg gaaacttccc gcaacacggg aaacagacaa
      181 atgaaatcgt caaacctcgc caacaggaat cgaacctgta ttttacgctt aggaggcata
      241 cgttctatcc gttgaactat ggcgagccga aatgaaaagg agattttaac cctttccgac
      301 gacaaaagac aagatgccgg ccgcaatcag cggcccgaca gggataccgc ccataaatgc
      361 caccccgata accgtcccga ttaacagccc tgtaattaaa acaggctgct gtcccatcat
      421 aaggcacgcc gcgtcccgcc agccaagcca cgaaaatacc gataaaaacg gcggatatca
      481 ttttaaaatt caaaaattcg gcaacgggag gaacctgcgc ctttcctgaa accaacggac
      541 tcaaaacccc tatggtcaaa agaatgatac cgagattcaa cccgtgcttc tcgaccaacg
      601 ggacaaactg tatcaatgcc gtctgctgca tcagcagcaa tatggttgcc gagatggtaa
      661 tcgaattgtt gttgctgacc acccccaaca gaatcagcgt aaccagaaac aggggaacaa
      721 aactgaaatt catcgcctca agacaccctg accaaagatt ccttcgccag ctgaatcatg
      781 gcatccttca cttcgctgtc gggcagggca tccaaggcgg taacggcaca atcgactgct
      841 ttgcgcgcct cgcctatcga atatgccaac gcatccgaac ggacgacata atcgtggatt
      901 ttctcaaaat agctgcgatc tgcattttcc aaagcagtac gcacatcgtt cgcaacctgt
      961 tcggaaccct gacgcatcag ataaatcaaa ggcaaagtcg gttttccttc cgccaaatcg
     1021 tcgccgacgt ttttgccggt ttcttcggtt tcgccagaat agtccagcac atcgtcaata
     1081 atttggaatg ccgtaccgac atacataccg tagtctttca acgcccgttc gtgttcgggg
     1141 gaagccttgc ccaaaattgc gccgacttga gcggcagctt caaacaattt tgccgtttta
     1201 tattggatga cttggatata ttgttcttcg gtaatgtccg tattgccgat gttcatcagc
     1261 tgcatgactt cgccctcggc aataatgttg gttgcatccg ccatcacttc caaaacgcgc
     1321 atactgcccg aggcaaccat cagttgaaag gcgcgcgtgt ataaaaagtc gccaaccaac
     1381 acagccgccg cattgccgaa cagattgttt gccgttgccc gcccacggcg caaatcgctt
     1441 tcatcgacga catcgtcgtg caggagggtg gaagtgtgga taaactcgac catcgccgcc
     1501 agcgaataca gtttctcgtc atcataaccg accgccttac ccgccaaaat cgtcataatc
     1561 ggacgcaggc gtttgccgcc cgcgctgatg atatatgtac cgatttgcga aatcagtgcg
     1621 acatcggatt gcaccgcacg gttgatgact tcattgactt tggcaaggtc ttcaggcaga
     1681 tgtcgctgga aatagggcag attctcgagc atggcagact ctcaggttta cgtttccgtc
     1741 aaacggcaga taccgcttga aagctgattc aaatagcggg caattatatc agcaaagcca
     1801 tcagggtaca tcttttcaga cggcatatgg ttatccgaac cgcgcaaact ttgacaaaac
     1861 aggcaaataa caatacaatg ccgagttccg cacaatggtg tgcggatatt aaccataacc
     1921 cttatggagt tgagtatgta cgcggtcgta aaaaccggcg gcaaacagta taaagtttcc
     1981 gtcggcgaaa aattgaaagt agaacagata ccagccgaac tcgacagcca aatcgaactg
     2041 accgaagttt tgatgattgc tgacggcgaa tctgtaaaag ttggcgcacc ctttatcgaa
     2101 ggtgcaaaag taacggctaa agtagtggca cacggtcgtg gcgaaaaagt ccgcatcttc
     2161 aaaatgcgcc gccgcaaaca ctaccaaaaa cgccaaggcc accgccaaaa tttcacccaa
     2221 atcgaaatcg tggcaatcgc ctaaccttaa aacttaggag cattacaaat ggcaagtaaa
     2281 aaagcaggcg gcagcacccg caacggtcgc gattcagaag ccaaacgctt gggcgttaaa
     2341 gcctacggca acgagctgat tcccgcaggt tccatcatcg tacgccaacg cggtaccaaa
     2401 ttccacgcag gcgacaacgt aggtatgggc aaagaccaca ctttgttcgc caaagttgac
     2461 ggttatgtcg aattcaaaac caaaggcgcg ctgaaccgta aaactgtcag catccgtcct
     2521 tacaccggtt ctgaagaata atccaaccga tacctgaagc cgcatctttt cacgatgcgg
//
LOCUS       DAWIVB010000207         2580 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWIVB010000207 DAWIVB010000000
VERSION     DAWIVB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.207"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            73..687
                     /locus_tag="WIX60_001933"
     CDS             73..687
                     /locus_tag="WIX60_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ5306144.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPILLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILVENNSFDMLWTVLG"
     gene            848..2509
                     /locus_tag="WIX60_001934"
     CDS             848..2509
                     /locus_tag="WIX60_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ5306145.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPDE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          505 a          650 c          668 g          757 t
ORIGIN      
        1 cggggttgaa gttgacatct acaaggttta atgtttgaat acatttaaaa atatatttga
       61 atgagatgtt ttatgattca aaatgtcgtt acttcaataa tcctgtattc tgggacagcc
      121 gtagacttac ttattatcct aatgttattt tttgccaaaa gaaaaagtag aaaagacatc
      181 attaacatct atttaggaca atttctaggc tctgttagtc tgatattgct aagtttactt
      241 tttgcatttg tcttagatta tattcctagt aaagagattt taggtttgct cggcttgatt
      301 ccaattctcc taggcatcaa agttttgctt ttaggagatt ctgatggaga ggctattgcc
      361 aaagagggtt tgcgcaaaga taataaaaac ctgatttttc tagtcgctat gattactttt
      421 gcaagttgtg gtgctgacaa tattggtgtc tttgtcccat attttactac cttaaattta
      481 gcgaatttga tagtggcttt acttaccttt ctagtcatga tttatctctt ggttttttct
      541 gcccaaaaat tagcacaagt cccttctgtt ggagaaactt tggaaaaata tagcagatgg
      601 tttgttgctg ttgtttattt aggattgggg atatatatcc tggttgaaaa caacagtttt
      661 gatatgctat ggactgtgtt gggctaggaa aaaatattat gaaaaagata gtatctgcaa
      721 agactgccat gtgcagtctt ttttgttgcc ggtctttttg tgtctgatgc cgtctgaagc
      781 agtctctgca cgacctttgt gcgaatattt gctacacttg gcaatccgtc cattttttct
      841 cacgcttatg ctgacctata ccccgcccga tgcccgcccg cccgccaaaa cccacgaaaa
      901 gccgtggctg ctgctgttga tggcgtttgc ctggttgtgg cccggcgtgt tttcccacga
      961 tttgtggaat cctgacgaac ctgccgtcta taccgccgtc gaagcactgg caggcagccc
     1021 cacccccttg gttgcccatc tgttcggtca aaccgatttc ggcataccgc ccgtgtatct
     1081 ttgggttgcc gccgcgttca aacatttgct gtcgccgtgg gctgccgact catacgatgc
     1141 cgcacgcttt gcaggcgtat tttttgccgt tatcggactg acttcctgcg gctttgccgg
     1201 tttcaacttt ttgggcagac accacgggcg cagcgtcgtc ctgattctca tcggctgtat
     1261 cgggctgatt ccagttgccc atttcctcaa ccccgctgcc gccgcctttg ccgccgccgg
     1321 actggtgctg cacggttatt ctttggctcg ccggcgcgtg attgccgcct cttttctgct
     1381 cggtacgggc tggacgctga tgtcgttggc agcagcttat ccggcagcat ttgccctgat
     1441 gctgcccttg cccgtactga tgtttttccg tccgtggcaa agcaggcgtt tgatgttgac
     1501 ggcagtcgcc tcacttgcct ttgccctgcc gcttatgacc gtttacccgc tgctcttggc
     1561 aaaaacgcag cccgcgctgt tcgcgcaatg gctcgactat cacgttttcg gtacgttcgg
     1621 cggcgtgcgg cacgttcaga cggcattcag tttgttttac tatctgaaaa acctgctttg
     1681 gtttgcattg cccgcgctgc cgctggcggt ttggacggtt tgccgcacgc gcctgttttc
     1741 gaccgactgg gggattttgg gcgtcgtctg gatgcttgcc gttttggtgc tgcttgccgt
     1801 caatccgcag cgttttcagg ataacctcgt ctggctgctt ccgccgcttg ccctgttcgg
     1861 cgcggcgcaa ctggacagcc tgaggcgcgg cgcggcggcg tttgtcaact ggttcggcat
     1921 tatggcgttc ggactgtttg ccgtgttcct gtggacgggc tttttcgcca tgaattacgg
     1981 ctggcccgcc aagcttgccg aacgcgccgc ctatttcagc ccgtattatg ttcctgatat
     2041 cgatcccatt ccgatggcgg ttgccgtact gttcacaccc ttgtggctgt gggcgattac
     2101 ccggaaaaac atacgcggca ggcaggcggt taccaactgg gcggcaggcg ttaccctgac
     2161 ctgggctttg ctgatgacgc tgttcctgcc gtggctggac gcggcgaaaa gccacgcgcc
     2221 ggtcgtccgg agtatggagg catcgctttc cccggaattg aaacgggagc tttcagacgg
     2281 catcgagtgt atcggcatag gcggcggcga cctgcacacg cggattgttt ggacgcagta
     2341 cggcacattg ccgcaccgcg tcggcgatgt acaatgccgc taccgcatcg tcctcctgcc
     2401 ccaaaatgcg gatgcgccgc aaggctggca gacggtttgg cagggtgcgc gtccgcgcaa
     2461 caaagacagt aagttcgcac tgatacggaa aatcggggaa aatatataaa aaacaacaga
     2521 ttgagccgaa tttctggatt aagtgccgga aatcgcgtat aattgcgcga ttaaaccttt
//
LOCUS       DAWIVB010000208         2565 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWIVB010000208 DAWIVB010000000
VERSION     DAWIVB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.208"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(12..182)
                     /locus_tag="WIX60_001935"
     CDS             complement(12..182)
                     /locus_tag="WIX60_001935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306146.1"
                     /translation="MAIMAYFGGFVRALFDLGVFERGGVKGRGKWFSRVWRRSEVLCL
                     MCGGIFSVKLIL"
     gene            242..2467
                     /locus_tag="WIX60_001936"
     CDS             242..2467
                     /locus_tag="WIX60_001936"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ5306147.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTSDIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVIVPEATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT          710 a          782 c          589 g          484 t
ORIGIN      
        1 aatttaaacc actataaaat caatttcacc gaaaatatgc cgccgcacat caggcacagc
       61 acctccgacc gccgccaaac gcgggaaaac catttccccc ttcctttcac gccgccgcgt
      121 tcaaacacgc caagatcgaa caaagcgcga acaaagccgc caaaatatgc cataatagcc
      181 acgcacattc cccgcgcaat gtgttccaaa agaaaaaacc aacctacaag agaataatcc
      241 tatgactcaa aaatccacca ttgtttatac ccataccgac gaagcacccg cgctggcgac
      301 ccaatcgctg ctgccgattg tgcaggcttt tgcccgccac gccgatattg atgtcaaaac
      361 cagcgacatt tctctctccg gccgtatttt ggcggcgttt cccgaatacc tgaccgaagc
      421 gcaacgcgta cctgatgcgc ttgccgaatt gggcgaactg gtgaaacaac ccgatgcaaa
      481 cgtaatcaaa ctgccgaaca tcagcgcgtc cgtaccgcaa ctgactgccg cgattaaaga
      541 attgcaatct aaaggctttg ccgttcccga ctatcccgcc gacccgcaaa ccgatgaaga
      601 aaaagccgta cgcgaacgct acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg
      661 tgaaggcaac tccgaccgtc gcgcccctaa agcagtgaaa aactttgcga aaaaaaatcc
      721 gcacagcatg ggcgcatgga ccaaagactc caaaacccac gttgccacca tgcaaagcgg
      781 cgactttttt cataacgaac aatctgttat cgtacctgaa gcgacttccg tatccatcgt
      841 attcaccgac aaacaaggca acaaaaaaga gctgcgcgag cccgtcgccc tgaaagccgg
      901 cgaaatcatc gacgcgaccg tgatgagtaa aaaagccctg ctcgccttcc ttgccgaaca
      961 agtaaaagat gcgaaagcaa aaggcgtgtt gttctcgctg cacatgaaag ccactatgat
     1021 gaaagtgtcc gacccgatta tcttcggaca cgccgtcaaa gtattcttcg cgcctgtttt
     1081 tgaaaaattc ggcgacaaac tggctgctgc cggcgtcaac gttaacaacg gcttcggcaa
     1141 cctgcttgcc aatctggaca aactggatgc ggacacccgc accgccgtcg aagccgaaat
     1201 cgccgccgtt tacgctgcca accccgattt ggcgatggtt gattccgaca aaggcatcac
     1261 caacctgcac gttcccagcg atgtcatcgt cgatgcctct atgcctgcga tgattcgcaa
     1321 ttccggccgt atgtgggaca aaaacggcaa agcgcaagac accaaagccg tgattcccga
     1381 cagcagctat gccggcgttt accaagcaac catcgacttc tgccgcgaac acggcgcatt
     1441 tgacccgaca accatgggta ctgtgcccaa cgtcggactg atggcgcaag cggcggaaga
     1501 atacggctcg cacaacaaaa cctttgaaat cgaagccgac ggccaagtcc aagtcattga
     1561 tgcggcagga aaagtcctaa tgcagcacga cgttgaagcc ggcgacatct ggcgtatgtg
     1621 ccaaaccaaa gacgctccgg ttaaagactg ggtacaactt gccgtcaacc gcgcccgtct
     1681 gagcaacacg cctgccgtgt tctggctcga cgaaaaccgt ccgcacgaca agagcctgct
     1741 cgccaaggtt aaagcctacc ttgccgaact ggataccaat ggcctcgaca tccgcgtcct
     1801 cgctcctgaa gaagccgcca agttcagctt gggtcgtctg aaaaacggcg aagacaccat
     1861 ctccgtaacc ggtaatgtct tgcgcgacta cctgaccgac ttgttcccaa ttttggaact
     1921 cggcacaagc gcgaaaatgc tgtctatcgt tccattgatg aacggcggcg gtatgtttga
     1981 aaccggcgcg ggcggttctg caccgaaaca tgttcaacaa ttcctcgaag aaaaccattt
     2041 gcgctgggac tcgctgggcg aattcctcgc actcgccgta tcgtttgaac atttggcgca
     2101 aaaaaccggc aatgccaaag cccaagtcct cgccgacact ttggatgcag ccaccgaaaa
     2161 actgctgttg aacgacaaat cgcctaaacg taaagccggc gaactcgaca accgcggcag
     2221 ccatttctac ctcaccctct actgggcgca agaattggcg gcgcaagaca aagatgccga
     2281 actgaaagcc gcatttacgc cattggcagc cgctttgacc gccgacgaag cgaaaatcgt
     2341 tgccgagctt tccgccgtac aaggcaaagc ggccgacatc ggcggctact acgccgccaa
     2401 tcctgaaaaa gccgcacaag tgatgcgtcc gagcgtaacc tttaatcagg cactaaacgc
     2461 cttataagca caaaggtaaa aatgccgtct gaacattcgt cgttcagacg gcattagtta
     2521 tccctatctg cctgattatg attaaacgcc gcacccattg gatat
//
LOCUS       DAWIVB010000209         2555 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWIVB010000209 DAWIVB010000000
VERSION     DAWIVB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.209"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(63..2225)
                     /locus_tag="WIX60_001937"
     CDS             complement(63..2225)
                     /locus_tag="WIX60_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ5306148.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFSIPTTEEKNGQKVDK
                     PMEQQMKDRADEDTVHAYKLSNPTKTDTGVYVEAIHDIGDFTLTGGLRYDRFKVKTHD
                     GKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTK
                     AERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHG
                     YELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFQN
                     PNLEIGWRGRYVQKATGSILAAGQKDRKGNLENVVRKGFGVNDVFANWKPLGKDTLNV
                     NLSVNNVFNKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          514 a          576 c          739 g          726 t
ORIGIN      
        1 aaattatcag aacagatgcc gtctgaaagg ctttcagacg gcattttttc gggatgtgcg
       61 ttttagaact tgtagttcac gcccaagcgt acatcacgtc ccacgcccgg cagggtattg
      121 gtccagcgtt ggctgtgcgg atagtagaac ttgttgaaca cgttgttaac cgaaagattg
      181 acattgagcg tgtctttgcc cagcggtttc cagttggcga agacatcgtt cacaccgaaa
      241 cctttgcgta caacgttttc caagttgcct ttgcggtctt tttgacctgc cgccaatatc
      301 gaacccgtag ctttttgaac ataacggccg cgccagccga tttccagatt cggattttgg
      361 aagcggtagg caagggaggc cgtccaagtg cggccgactt gtgcgccaaa ttcaggattc
      421 gcgctcaaca gcttgtcttt gtgcgtatcg taaaagcgcg gtttgctgtg gctgacgccg
      481 actttggcag tcaggccgcc ggtgcggtag gacgcgccca attcgtaacc gtggtttttg
      541 atgtaaccgg cattgacggc ttcacggacg gcgacagagt cgtggcggtt ttgcggattg
      601 gcaagcgcgt ctttgatggt ctgccagaag tagctgccgt ttgcggcaaa cgtgccgtcg
      661 ttgtagttga agccgatttc ggtattgcgc gcgcgttcgg cttttgtgcc gtcggcaatc
      721 gagatgatgc cgcgtttacc gtgggtttgc agcgcgtcat acaggcgcgg gctgcggctg
      781 gcgtagttgt ggctcgcgct gaagctccag tgttcgtgcg gctgccaaat cacaccgaaa
      841 ctcgggttaa ggttgctgct tgaaacggtt ttgccgtcat gggttttcac cttgaagcgg
      901 tcgtaacgca gcccgccggt cagcgtgaaa tcgccgatgt cgtgaatggc ttcaacatat
      961 acgccggtat cggttttggt cgggttggaa agtttgtagg cgtgaacagt gtcttcatct
     1021 gcacggtctt tcatttgttg ttccatcggt ttatcgactt tttgaccgtt tttctcttct
     1081 gtcgtcggga tggagaattt cgagttcaaa aatgcttgcg gtttgatttc ctgatggcgg
     1141 tagttgatac cgtatttcaa cagggtttgt tcggcaaggc ggctgtcgaa gttgaagttc
     1201 gcaccacgag tggtgattcg ggtatggttg gggcctttta cattgcctgc gtagccggtg
     1261 ccgctgtcat cggcggaata gcgttctttt tccaacacat aggcgttggc atccagtttt
     1321 tcgacaaagc ccaggttttt acccgtgtac gccaagttgg tgttggattg ggtagtttcg
     1381 cggtaagcag gggcttggcg gtcaatattt atccgtgaac ttttgtcgcc gacggtaaat
     1441 tcttcacgca cagtgcggat gccccggtgt tggtctttca tgtggctcaa cacgatgcgg
     1501 tggtcgtcgt cgccgaaggt tgttccgatt ttggcgaggt agctgcgttt gtccagcgcg
     1561 ctgtacggta cggttttgcc gccgttgaca ttgcggaaac ctttgccggc ttcgtaatct
     1621 ttttcatcgt tgcggttgta agagaacaag ccgtcgaagt tgccctcttt tccgaatacg
     1681 cttgcgccgt agcttacgcc ttcgttgctg gcaaagccgc tgttgaggcg cacgccccag
     1741 tttttatcca agcctttgag caggtcttgg gcatcgacgg ttttggcgat gatcgcgccg
     1801 ttggtcgcgc cgataccggc agaggcggaa cccgcgcctt tttgtacgga aacgacttta
     1861 accaaagcgg gatcgacaat aaatctgcct tggtggtaaa ggatttggct gtcggaatag
     1921 gcgttgtcca ccttgatgtc gacagagttc tgacccatgc cgcgcagcgt caggaattgg
     1981 gacgtgccgt tgccgccgcc gaaatcgatg gacggctctt cttttaagag ttcgcgcata
     2041 tcggttgcgg tgctttcgtc tttttgttgc agcgtaacga tgttggtacg gattttgctg
     2101 ccttggcggt cgccttttac agtaacggta tccagtacga ccttggcatt attttctgcc
     2161 gcgtgggcaa aacctgccgc aagtgtgagc gagagcaggc tgagacggaa caatggggta
     2221 ttcattcaat cgtcctcttg agtatgaagg gaagtaaatc caaaccgtta agatttggca
     2281 ggataagaaa aataattaat aattattttt atttatatta acaggggggg gggggatttt
     2341 tgcaaagctg attatcgttt ttatttgcga agtgttgttt tttgttgaca ggttttgtcg
     2401 gaaatgtaaa aaaacggcgg gaatatagtg gattaacaaa tgcgggaatg acgaagcctg
     2461 tgcgggaatg acggcggagc ggtttctgtt ttttccgata aattcctaaa acttaaaatt
     2521 tcatcattcc cgcaaggaca gaaaaccaaa aacag
//
LOCUS       DAWIVB010000210         2546 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWIVB010000210 DAWIVB010000000
VERSION     DAWIVB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.210"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(118..1569)
                     /locus_tag="WIX60_001938"
     CDS             complement(118..1569)
                     /locus_tag="WIX60_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEZ5306149.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKFGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1592..2212)
                     /locus_tag="WIX60_001939"
     CDS             complement(1592..2212)
                     /locus_tag="WIX60_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ5306150.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            complement(2411..2546)
                     /locus_tag="WIX60_001940"
                     /pseudo
     CDS             complement(2411..2546)
                     /locus_tag="WIX60_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          798 a          602 c          585 g          561 t
ORIGIN      
        1 gaataagaat tcgggacaaa atgtttaaac cattttgtcc cgactgctgt gatgcggttt
       61 ttttgaacta gcaggacgtt acctaaaacc tgcgttgcct gaaacccttt aagacggtta
      121 aatccctctg ccgtatttgt attctacaat catattatcg tcggtaataa cttccgcccc
      181 agccgaaggt tccgtcatct gaatcagcat acgagagaca accttttgtg ctgcagcatc
      241 cacggtgctg ctgtcaaata cgtgcctgcc gctttccggc caaatcaacc gggagagacg
      301 ttgcttgagc agttctttat tagggaaaac taccggggtt gccgagccga ctaccatatg
      361 cccatagcgg tatgcatagg gaatactgtg tacggcggta gcaaaagcat gcgggctgtg
      421 cgtggtatta aacattacaa taccatccgg ggtaaggtgg ctttgcacct gttttaaaaa
      481 ttccgcactc aacaggttgg tggaataggc acgccagtac caagtcgtat tcatcaaaat
      541 caggtcgaat ttttcatcag gatggcgacg cagccatttc ctaccgtcat ccaatacaat
      601 ttcaacacgt ttgtcctgca aaagcggggc gatttgcggc tcgtccgcga taaggctacg
      661 gtatgccgga ttgatttccg caacaatcat cgactgcatt tccggaatgg cagacaagac
      721 gcgcgcccac gaacctgtac tcaatccaac gacgaaaatg cggcgtatgc caaacttcag
      781 ggagggtagc agataggcac gttcgatgcc gttgacacta ttgaatacat cggtattgta
      841 tgcgccgtcg tatacattcg ccccataaac aaccttatca ccatctctat ggtaaaccgc
      901 aacaatgccg tgtttgtttt caatcagcct atccggacgg tcagcaatat tttgaaagac
      961 agaatccggc agtaggaaca tgaggatgcc gaacattagg gaaactgcta ccgacactgc
     1021 attcagtcgg agactttttt ggaacagtgt acaaaacaaa gggacagcag cagaaatcaa
     1081 acagatgagc aggtaaatct gttgggtgga caagaaatca agtatcacaa agccgataag
     1141 gaccggaccc aatgcactgc cggcaacgtt ggcgaaataa acattggaaa cctgtcgtcc
     1201 ggatttgttg ccatccgtac ccacatggtg tacgagcggg aaaatcaacc ctctgacgac
     1261 ggcagacagg gtaatgaaga taccggcgtg gtggacgaag ccggaaaaac ccgtcaacaa
     1321 ccacgcagca cccaaaatca aaaagtcggc aatacccgcc cacaagaagc actgcccgat
     1381 aaagggaata tcaacaaagc ggctgcggca aatccgtttg ccaaaatacg cgccgacggc
     1441 gataccggtc agaaaacagg caagagtaaa tgaaaatgcc tgaggcacgg actgtgctgc
     1501 gaacgaaaac atccttaccc acaagacttc tatacccaag ctcaataagc cgctaaggaa
     1561 agaaagcata taaatcaaac tagtattcgg tttagtgttc actatatcca tcctttctgt
     1621 aacgcagcca tactgaagca gcaatcagaa ggttaaagca ggctgtcagc gcaatggttt
     1681 gggagagggt aaaaaagacg tagaaaaatt cggcggcggc aagcgatccg agtgccgcac
     1741 ccaaagtgtt gaaaaaatat aaggtaccga tagactcgcc aacattatgt attttccggt
     1801 taaaaaaaca ggtcagcaag ggcaaggtcg cgcccatcat aaaggtagga agcagcaata
     1861 agaggaaatt ggcagcagcg atgatgggca aatcagcctc aactaaaaga tgccccaagc
     1921 cggaaatcag acccctgctt accaaaccga acagaccgat ggatacttca gcgatgcaaa
     1981 acagggggat gatacttgaa ggaaaacggt cagcaatgcg tccaccgaaa tacgcaccta
     2041 cacccaagcc gaccataaat acagaaataa tgacagtaat cgaactcaaa tcgataccta
     2101 tgtgactgaa tagaagcctc tgccagctga cctggtaaat cagggcgcag aatccagagg
     2161 cgaaaaacac cagcgacaat cctttcaggg ttttacttgc ggtcgaattc atattttcta
     2221 ttttcaaata ggcggaacta taaatgaaat gtatcaatgt gtaaacattt gtattaaccg
     2281 taggaaatca ccgtattgct caaactgcag gccgggaaaa taaactcgta agcccgcaaa
     2341 cggcgtatca ctgttcaaac tgctgtgcgg cttcacggtg ttataaaaat aatggggctg
     2401 tcctggacaa ctaagataaa ctcgatttta ctaattgttt taaaatggaa caagaacttt
     2461 tatctcactg ttgttaaaac gccattcgca ctcctttaaa tacagctcaa aatgcgcttt
     2521 gggaatgccg ttagacttgc gtaaat
//
LOCUS       DAWIVB010000211         2509 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWIVB010000211 DAWIVB010000000
VERSION     DAWIVB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.211"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            167..790
                     /gene="lolA"
                     /locus_tag="WIX60_001941"
     CDS             167..790
                     /gene="lolA"
                     /locus_tag="WIX60_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ5306151.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1063..2202
                     /locus_tag="WIX60_001942"
     CDS             1063..2202
                     /locus_tag="WIX60_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEZ5306152.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            2335..>2509
                     /locus_tag="WIX60_001943"
     CDS             2335..>2509
                     /locus_tag="WIX60_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ5306153.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIESILSNENVELILVNDGSKDG
                     SEDICYKYIDKISN"
BASE COUNT          687 a          726 c          620 g          476 t
ORIGIN      
        1 gcggcacaca ttaaagggca gcgtggcgcg ccgccttttc cggtgggcaa aaaatcagcc
       61 ctcggaaaac gcggtttgca aaatgcaaac cgcccgtaac gccgcccgta tgattgtttt
      121 gctgcgccga tactttacgc cacactcatc ccgacaagga aaaataatga tgaaaccgca
      181 caacctgttc caattcctcg ccgtttgctc cctgaccgtc gccgtcgctt ccgcacaggc
      241 gggcgcggta gacgcgctta agcaattcaa caacgatgcc gacggtatca gcggcagctt
      301 cacccaaacc gtccaaagca aaaagaaaac ccaaaccgcg cacggcacgt tcaaaatcct
      361 gcgaccgggc cttttcaaat gggaatacac caaaccttac aggcaaacca tcgtcggcga
      421 cggtcaaacc gtttggctct acgatgttga tctggcacaa gtgaccaagt cgtcccaaga
      481 ccaggccata ggcggcagcc ccgccgccat cctgtcgaac aaaaccgccc tcgaaagcag
      541 ctacacgctg aaagaggacg gttcgtccaa cggcatcgat tatgtgctgg caacgcccaa
      601 acgcaacaac gccggctacc aatacatccg catcggcttc aaaggcggca acctcgccgc
      661 catgcagctt aaagacagct tcggcaacca aacctccatc agtttcggcg gtttgaatac
      721 caatccccaa ctctcgcgcg gcgcgttcaa gtttaccccg cccaaaggcg tggacgtgtt
      781 gagcaactga tgccgtccgc cccgatgccg tctgaaagcc gccgaggctt cagacggcat
      841 ttttacgcag gcggaacaat gtcccgcatt accgcccgat cgggcaccgg aacggcaaac
      901 cggtgaaaat taacggttgc gccccggctg tttttgccgt ttaatgcaaa ccttgctgca
      961 ccaagggcca agaaagccga ccggccgccc ccacagcttc cgatgcaggc ggcccgtccg
     1021 tccctgcaat gttttttatt tttgaacgaa aggtcgaaaa ccatgaaaaa aacactggtg
     1081 gcggcggcaa tcctgagcct cgccttgact gcgtgcggcg gcggaagcga taccgccgcc
     1141 caaaccccct ccgccaagcc cgaagccgaa caatcgggca aactcaacat ctacaactgg
     1201 tcggattatg tcgatcccga aaccgttgcc gcctttgaaa aagaaaccgg catcaagacg
     1261 cgttccgatt attacgacag caacgaaaca ctggaggcaa aagtcctgac cggcaaatcc
     1321 ggctacgacc tgaccgcgcc gtccatcgcc aacgtcggcc ggcaaatcaa agcgggcgcg
     1381 tatcagaaaa tcgacaaggc gcaaatcccc cattacggca acatcgataa agatttgctg
     1441 aaaatgatgg aagccgtcga tccgggcaac gaatacgccg tcccctattt ctggggcatc
     1501 aataccttgg caatcaatac ccagcaggtg aaaaaagcat tgggtacgga caagctgccc
     1561 gaaaacgaat gggatttggt gttcaaaccc gaatacaccg ccaaactcaa atcctgcggc
     1621 atcagctatt tcgacagcgc aatcgaacag attcccttgg cgttgcacta tttgggcaaa
     1681 gaccccaaca gtgagaatcc cgaagacatc aaagccgccg tcgatatgat gaaagccgtc
     1741 cggggcgacg tgaaacgctt cagctcttcc ggctatatcg acgatatggc ggcgggcaac
     1801 ctgtgtgccg ccatcggtta cggcggcgat ttgaacattg ccaaaacccg tgccgaagaa
     1861 gccgcaaacg gcgtggaaat caaagtattg accccgaaaa ccggcgtggg cgtgtgggtg
     1921 gattccttta tgattccgcg cgacgcgcaa aacgttgcca atgcccaccg ctatatcgac
     1981 tacacgctcc ggcccgaggt ggcggcgaaa aacggcagct tcgttaccta cgcgcccgcc
     2041 agccgtcccg cgcgcgagct gatggatgaa aaatacacct ccgacgcatc gattttcccg
     2101 aacaaagaac tgatggaaaa aagtttcatc gtatcgccca aatccgcaga atccgtcaaa
     2161 ctgggcgtga agctgtggca agggctcaaa gcgggcaaat aaccggaatc cctgccgtct
     2221 gaaacctttc gggcggcagg aaacggcgcg tccgttatca aacagggggg cgtttcccct
     2281 cctgccggtt atgattgggt taagattaaa atgatttagt aaaatgagaa agatatggat
     2341 ttaagtatcg tagttcctat ttataatgtc gaaagttatt tggaagcgtg tttaagttcc
     2401 atagaatcta tattaagtaa tgaaaatgtc gaacttatcc ttgtgaatga cgggtcaaaa
     2461 gacggaagtg aagatatatg ttacaaatat atagataaaa tatcaaaca
//
LOCUS       DAWIVB010000212         2436 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWIVB010000212 DAWIVB010000000
VERSION     DAWIVB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.212"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            35..1390
                     /locus_tag="WIX60_001944"
     CDS             35..1390
                     /locus_tag="WIX60_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ5306154.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WIX60_001945"
     CDS             1394..2404
                     /locus_tag="WIX60_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ5306155.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHMPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          438 a          661 c          741 g          596 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcagatg agccgcatct tccagacgct
      181 cggtgccgat ccgcacagcc tcggctggtt ctttatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggtaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggct tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggtctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgat gatgcggctt cctatgcgga caatccgttt cagattaaca aacagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatg ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaatggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg accttt
//
LOCUS       DAWIVB010000213         2431 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWIVB010000213 DAWIVB010000000
VERSION     DAWIVB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.213"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(228..1862)
                     /gene="groL"
                     /locus_tag="WIX60_001946"
     CDS             complement(228..1862)
                     /gene="groL"
                     /locus_tag="WIX60_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ5306156.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM"
     gene            complement(1955..2245)
                     /gene="groES"
                     /locus_tag="WIX60_001947"
     CDS             complement(1955..2245)
                     /gene="groES"
                     /locus_tag="WIX60_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ5306157.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGSRRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          496 a          636 c          631 g          668 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaacaaaa atgcaaagaa
      121 cggcgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaaccgcac
      181 ggcgttgacc gtgcggtttt ttatctgaaa gcttcagacg gcattgctta catcatgccg
      241 cccataccac ccatgccgcc catatcaggc acagccggtt tgtcttcggg gatttcagcg
      301 atcatgcaat cagtggtcag catcaagccg gcgatagatg cggcgtgttg cagcgcagaa
      361 cgggttactt tggcggggtc gagtacgccc atttcgatca tatcgccgta ttcgccgctg
      421 ccagcgttgt aaccgtagtt gcctttgcct tccaatactt tgttcacaac cacgctgggt
      481 tcgccgcctg cgttggcaac gatttggcgc agcggagact caacggcgcg caagacgatt
      541 tgtacgcctg cgtcttggtc ggcattgccg gtgtgcaggt tttccaaagc agcacgggca
      601 cgcaacaggg ctacgccgcc gcctgcaacc acgccttctt caacggctgc gcgggtagcg
      661 tgcagcgcgt cttccacgcg gtcttttttc tctttcattt cgacttcggt cgcggcaccg
      721 actttgatga ctgccacgcc gcctgccaat ttagccacgc gctcttgcag tttttctttg
      781 tcgtaatcgc tggttgcggt ttcgatttgt tggcggattt cggcaacacg cgcttcgatt
      841 tgggctgcgt cgccaaagcc gtcgatgatg gtggtgtttt ctttaccgat ttcgatgcgt
      901 ttggcttgac ccaagtcgtc caaagtcgct ttttccaaag acagaccgac ttcttcggaa
      961 atcaccacgc cgccggtcag gatggcgatg tcttgcaaca tcgctttgcg gcggtcgccg
     1021 aagccagggg ctttgacggc aacggttttc aggatgcctc ggatgttgtt cacgaccaaa
     1081 gtcgccaagg cttcgccttc tacgtcttca gcgataatca acagcggacg gctggctttt
     1141 gccacttgtt ccaaaacagg cagcaggtcg cggatgttgc tgattttttt gtcgaacaac
     1201 aatacaaacg gattgtccaa agcagcgatt tgtttttccg catcgttgat gaagtaagga
     1261 gacaggtagc cgcggtcgaa ctgcatacct tcaactacgt ccagctcgtt ttccaaagac
     1321 ttgccgtctt caacggtaat cacgccttct ttgccgactt tttccatcgc ttcggcgata
     1381 atcgcgccga cttgttcgtc ggagttggcg gaaatagagc cgacttgggc gatttcttta
     1441 gaagtgtcgc aaggtttggc gatgtttttc agttcgtcaa ccaaagcggc gacggcttta
     1501 tcgataccgc gtttcaggtc ggtcggattc atacctgcgg taacatattt cataccttcg
     1561 gcaacgatgg attgcgccag tacggtggcg gtagtcgtac cgtcgcctgc cacgtcgttg
     1621 gttttggacg caacttcttt caccatttgc gcgcccatat tttcaaactt gtctttcagt
     1681 tcgatttctt tggcgacggt tacgccgtct ttggtgatgt gcgggccgcc gaatgcgcgg
     1741 tcaacgacta cgttgcgacc tttggggccc aaggttacgc ggacggcgtt tgccagaatg
     1801 ttcacgccgt ttaccatttt ttgacggact tcattgccga actgtacgtc ttttgctgcc
     1861 atttcaattc tccaaaaatc attaaaactg tctgataaaa ccgtttatgc cgtctgaagg
     1921 cggtttgccg tttcagacgg catcgtgtcc gtatttattt ttcaacgatg ccgaaaatat
     1981 cttcttcgcg cattaccaac agctcttcgc cgtcggcttt tacggtttgg ccgctgtatt
     2041 tgccgaagat gattttgtcg ccgactttga catccagcgg acggcggctg ccgtctttac
     2101 cgattttgcc cgcgcccacg gcgatgactt cgcccatatc gggtttttcg gcggccgcac
     2161 ccggcaaaac gatgcccgat gcggtttttt cttcagcttc caagcgtttg acgacaacgc
     2221 ggtcgtgtaa aggacggatg gtcatattta tgctccgata aaatagtttg aaaacaatca
     2281 tctgcccgaa cggttcaggc agattgaagt ggaaaccgga cagccgtcaa gcagctgccc
     2341 gtataagtcg gcaaaattag ggtgtgtgcg ggtaaattca agtgaggcgg aaaaaattta
     2401 tttccgccgt tttttatagt ggactaaatt t
//
LOCUS       DAWIVB010000214         2362 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWIVB010000214 DAWIVB010000000
VERSION     DAWIVB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.214"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..372
                     /locus_tag="WIX60_001948"
     CDS             <1..372
                     /locus_tag="WIX60_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ5306158.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETEGLGL
                     NVISHKILGKDHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQP
                     CICGSGKKFKACCGKYLKPVA"
     gene            complement(590..1384)
                     /locus_tag="WIX60_001949"
     CDS             complement(590..1384)
                     /locus_tag="WIX60_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ5306159.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1488..2213
                     /locus_tag="WIX60_001950"
     CDS             1488..2213
                     /locus_tag="WIX60_001950"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ5306160.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          624 a          749 c          525 g          464 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactaccgtt
       61 ccggcgcaac aggcattcct cgatgccgcc gaactgatgc aatggagtat agaaaccgaa
      121 gggctgggct tgaacgtcat ctcgcacaag atactcggca aagaccacgc ccaagtcgaa
      181 tttgaagcct acttccgaga cggacaacac cgatccgcgc atcacgaact gtccggcttc
      241 gtcaacatcg gcggacaatg gtattttatc gatcccaccg ttccgcatcc tgcgatgaaa
      301 caaccctgca tttgcggatc aggcaaaaaa ttcaaagcct gctgcggcaa atatctgaaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat tttcaacgtg caaaaaaaac
      421 cattcatacc aagggtaagt atgaatggtc aatacattgc gggaaaacgt cttacttgct
      481 gcactgccga aaagggagaa acggcagcgg taatcagcgg aaaggattgt acccgaatta
      541 atattaagaa acgttaatcg cgaaaatata ttaacaaacc tgttgaaacc tattggtttt
      601 cccgtatcca cccgacccag cgttcaaaca gcttcggttc gagcgcggca acgaccgagc
      661 gtttgaacac gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc
      721 ccgcctcctc gaaaatcaac gcgccggcgg cataatccca cagcttctgc ccgccgtgaa
      781 cataaacatc ataacgcccg cacgccagat aacaccaatc caacgtactg ctgcccatac
      841 tccgtatcgt tccaaaaggc gcgagcgtac tcatacggct ggaaagtttg cccgaacgca
      901 gatatttgat ttccacgccc gcaatcgcct cattgagttt tttatccacg aggcgcaggg
      961 gcagacgcgt cccgtttaaa aacgccccct gcccgcgttc ggcataaaaa cattcgccgc
     1021 tgactgggtt gtagattacg cccaactcgg cgcgcccgtt gcggacaaac gccaccgata
     1081 ccgcaaaatg cggcagcccg ttgacaaaat tgttcgtccc gtctatcgga tcgacaatcc
     1141 acagcccctt ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt
     1201 cctcacccaa catcggactg tcgattaaaa gcggcaacgc ggcggcaaaa gccgtctgcg
     1261 cggcaatgtc cgcctcgctc aacatcgaac cgtcttcctt gcggtgagac ggcgtattca
     1321 aaaaacgcgg cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta
     1381 acacttccta ctgtcctcat attttgaact tgcggcgcgc gaacgtataa tgtccgcttc
     1441 catcacgccg ctgcgacgga ttataaccgt ccgaaccgcc aaaaactatg ccccgattcc
     1501 acctgcccga aaacctttcc gtcggacaaa ccgtcgccct gcccgacaac atcgtccgcc
     1561 acctcaacgt cctgcgcgtc cgccccaacg aaaacatcac cctcttcgac ggcaaaggca
     1621 aggcacacgc cgcacggctg accgttttgg aaaaacgccg cgccgaagcc gaaatcctgc
     1681 acgaagacac aaccgacaac gagtccccgc tcaacatcac actgatacaa tccatctcct
     1741 ccggcgatcg catggatttc accctgcaaa aaagcgtcga actcggcgta accgccatac
     1801 agcccgtcat cagcgaacgc tgcatcgtcc gcctcgatgg ggaacgcgcc gccaaacgcc
     1861 tcgcacgctg gcaggaaatc gtcatctccg cgtgcgaaca aagcggcagg aacaccgttc
     1921 cccccgtact gcccatcatc ggctaccgtg aagcactcga caaaatgccg tctgaaagca
     1981 ccaagctgat tatgagcatc aaccgcgccc gcaaactcgg cgacatacgc caaccgtccg
     2041 gcgcaatcgt ctttatggtc gggcccgaag gcggctggac agaacaggaa gaacaacagg
     2101 catttgaagc tggctttcag gcggttacac tcggcaaacg gattttacgc acagaaaccg
     2161 ccccactcgc cgccctcgcc gccatgcaga cgctttgggg cgatttcgca taaacagaaa
     2221 tgccgtctga aacccgttca gacggcattt tgcagccgat taagatagta ggttcaaata
     2281 agatttcccg tgtcgtcatt cccgcgaaag cgggaatcta gaaacgaaaa actacagaga
     2341 tttatccgaa acaacaaccc tc
//
LOCUS       DAWIVB010000215         2308 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWIVB010000215 DAWIVB010000000
VERSION     DAWIVB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.215"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WIX60_001951"
     CDS             complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WIX60_001951"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ5306161.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLDAQKQLREQGILNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1627..2193
                     /locus_tag="WIX60_001952"
     CDS             1627..2193
                     /locus_tag="WIX60_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ5306162.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLCLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          526 a          559 c          662 g          561 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc
       61 ggcgttcttt gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt
      121 tggcaacgaa gttgtccaag tctggcagca gggaaacggc gcggccgctg ttgatgatga
      181 cgaccaccgc catcggtttg tcgcccagcc aataacctgc aagggcgcgg acattgttga
      241 gcgtgccggt ttttaagcgc aacagcccgc cgctttgttt gaagcggttg cgtaaagttc
      301 cgtctgtgcc ggcgatgggt agcgtgtcga tgaaatcttg tgcaaacggg ctgaaataag
      361 ccgtttccaa catttgcgcc atcattctcg ccgttaccct ttcttttctg gacaggcccg
      421 aaccgttttc caaaaccaaa tccgcaacat cgatgcccga tacggcaagt tcgcgccgga
      481 cggcagacgc cgcctgttcg gaaacggcgg gcagtttgcc gtcgccgccg agtttgagga
      541 agacggaacg cgcaattaga ttgtccgaac gcttgttcat gtccgtcaaa atttctttca
      601 tcggtttggc gtgtgcaacg gcaagtgtct gcgcgccttc cggcgtgtcg gctatgccga
      661 taccgtctga aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca
      721 gttcgtcaag cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat
      781 tgccgcgcaa tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgatcgaag
      841 ggcaggcagc ttgggaggcg gtaattttca agttgttttg ggcgaaaata tgcggcaaag
      901 gcggatcggt gaggatgtcg gtactgccgg cggcattgcg ttcggcgcgc accataacca
      961 taccggcaga cagcatagtt ggattggggg gcgtcataaa cggcgaaccg ctgtcggctt
     1021 cgaaatcgtc ggggctgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg
     1081 tgatattgag tatgccttgt tcgcgcaact gtttttgagc atcaagcagg ttttcctgat
     1141 tgaaaacggg gtcgccgctg cccgcccaat ataggtttcc gtcaagcgtg ccgtcgttta
     1201 ccgtaccgtt gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg
     1261 cggcaaacgc ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt
     1321 ggtcaatgat gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg
     1381 gaatgcggcc ggtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg
     1441 ccgctgtttt ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca
     1501 aagccggata agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat
     1561 atataataat cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga
     1621 atgattatgg ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg
     1681 cgcaaagtgg cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtt
     1741 gaaatcggac atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg
     1801 ccgctacccg aaagctacac ggctttccgc gaaacgatta aggcttcgga cggcattttg
     1861 ttcgttacgt ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc
     1921 ggctcgaaac cgaatgccga cgtggcgtgg aaaaacaaac cggcaggcat catcagccat
     1981 tccgtcggca agatgggcgg ttacagttcc caaaaaaacc tgtgccttgc gctgtcttat
     2041 ttcgatatgc ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacgctgttt
     2101 gatgaaaacg gcaaattgat tgactcggca agggattttg ttcagtcata tattaatcag
     2161 tttgtcggtt tgattgaaag aaatgccaaa taaacaaacc aagtcgaaag gccgcaaaac
     2221 cggttcgggt tttgcggctt ttttatatgg gctgggcagc gtcaaggttt gccgtttgcc
     2281 ggataatgcg cggcaaatga ccggcgtg
//
LOCUS       DAWIVB010000216         2285 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWIVB010000216 DAWIVB010000000
VERSION     DAWIVB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.216"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            19..747
                     /gene="rpe"
                     /locus_tag="WIX60_001953"
     CDS             19..747
                     /gene="rpe"
                     /locus_tag="WIX60_001953"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ5306163.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(770..1801)
                     /gene="ruvB"
                     /locus_tag="WIX60_001954"
     CDS             complement(770..1801)
                     /gene="ruvB"
                     /locus_tag="WIX60_001954"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ5306164.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1883..2191)
                     /locus_tag="WIX60_001955"
     CDS             complement(1883..2191)
                     /locus_tag="WIX60_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306165.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          455 a          545 c          738 g          547 t
ORIGIN      
        1 gatattgaag gaaaaaacat gactacttac cgtatcgcac ccagcatttt atcggcagac
       61 tttgcccgcc tcggagaaga ggtggaaagc gtcattgcgg caggtgcaga tctgatccat
      121 tttgacgtga tggacaacca ttatgtgccg aacctgacct tcggccctat ggtttgcgcg
      181 gcgttgaagc cttatgcaag cgtgccgatt gatgtgcatc tgatggtcga acccgttgac
      241 gacctgattc agtcgtttgc caaagcagga gcatcaatca tcacgttcca tcccgaggcg
      301 agccgccata tcgaccgcag cttgagcctg attcgtgata tgggctgtca ggcggggctg
      361 gtgttgaatc cggcaacgcc cgtatatctg ttggaaaacg tattggacag gctggatatg
      421 gttttgctga tgtcggtcaa ccccggattc ggcggacaaa gcttcatccc gcacaccctt
      481 gaaaaaatcc gccgggtgcg ggcgatgctg gatcggtacg aagcacaaag cgggcggcac
      541 atcgccatcg aagtggacgg cggcatcaaa accgacaata ttgccgccgt tgcccgagct
      601 ggtgcggata cctttgttgc cggttctgcg attttcggca aaccagacta taaggcggtg
      661 attgatgcga tgagggcgga actggaaaaa agcggcaggc tgaaccttgc gccaatgatt
      721 gaaatagaaa tgccgtctga aaattagctc tgtttcagac ggcattgcgt tatttttcga
      781 cgggcagccc gaaatgcagg taggcgcgtt cggtcgccat cctgccgcgc ggggtgcgtt
      841 gcaggaagcc ttgttggata aggtagggtt cgataacgtc ttcgatggtg tctgtagatt
      901 cgccgatggc ggcggcaaca ttgtccaaac cgaccgggcc gccgccgaat ttgtgcaaaa
      961 cggcttcgag aaatttcctg tccatcacgt ccagcccctg cgcgtccacg tccagcatac
     1021 ttaaagcggc atcggcgatg ccgccgtcga ttgtgccgtt gtttttcacg tcggcgaaat
     1081 cgcgcacgcg tcgcaacagg cggttggcga tgcgcggcgt accgcggctg cgtttggcga
     1141 tttcttccgc gccttcttcg gacatatcga gctgcaacag ttgtgccgaa cggctgacga
     1201 tggtggtaag gtctcggttt tcgtaaaact caaggcggga gacgatgccg aagcggtcgc
     1261 gcaacggatt ggtcagcata ccggcgcggg tggtcgcgcc gatgagcgtg aagggcggca
     1321 ggtcgatttt gacggaacgg gcggcgggtc cttcgcctat cataatgtcg agccggtagt
     1381 cttcgagcgc gggatagagg atttcttcga caacagggct gaggcggtgg atttcgtcga
     1441 tgaacaatac atcgtgcgga tcaaggttgg tcaaaagggc ggcgaggtcg cctgcgcgtt
     1501 cgaggacggg gccgctggtt tggcgcaaat ttacgcccaa ttctttggcg atgatgtgcg
     1561 ccagtgtggt tttgcccagt ccgggcgggc cgaagagcaa aacgtggtcg agtgcttcgc
     1621 cgcgtttttt ggcggcttgg atgaaaatgg caagctgttc tttggctttg tcttgcccga
     1681 tgtagtcgtc cagcgttttg gggcggaggg cgcgttcgag cagttcttcc tgtgcggagg
     1741 cggtttgggc ggcaacgatg cgttgcggtt gcgcggcggt caggttgtcg gtttgcagca
     1801 tagtgttccc tttgtcgggt atgccgtccg aacggtcggc ggcgtttcag acggcattga
     1861 aaagataacg gtcaaaagcg ttttatccgc gggtttcgcg ccaaacgaga tagtcgcgca
     1921 ggggcggcat aggcgggttg atgcagtggg ggcagatgcc ggtggtgtct tcgtcgtcgc
     1981 tgtcgatgtg gtaggcgttg gggtcgaagc ggatttgctg catgacggct tgcgccaatg
     2041 cctgcgcctg aacaacgtcg aaattgaatt tttcgaggat ttcgttcaac aggcggattt
     2101 cgccaccgct aaattccgca ctgtgtttga ggatgaggcg ttgataatct tcgttgttga
     2161 ttttaggcaa taaatcggtt ttcagggtca tgattttatg tcgggggcgg aatggctgaa
     2221 gcgtgaatta tagcggatat ggcggcggct gttgcaaagc ttggattggg tatcgtcctc
     2281 aaaac
//
LOCUS       DAWIVB010000217         2280 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWIVB010000217 DAWIVB010000000
VERSION     DAWIVB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.217"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            234..2009
                     /gene="nhhA"
                     /locus_tag="WIX60_001956"
     CDS             234..2009
                     /gene="nhhA"
                     /locus_tag="WIX60_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NhhA"
                     /protein_id="HEZ5306166.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVKTAVLATLLF
                     ATVQASANNEEQGEDLYLDPVQRTVAVLIVNSDKEGTGEKEKVEENSDWAVYFNEKGV
                     LTAREITLKAGDNLKIKQNGTNFTYSLKKDLTDLTSVGTEKLSFSANGNKVNITSDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKKRAASVKDV
                     LNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGA
                     KTSVIKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSV
                     SLGAGADAPTLSVDGDALNVGSKKDNKPVRITNVAPGVKEGDVTNVAQLKGVAQNLNN
                     RIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGGN
                     WIIKGTASGNSRGHFGASASVGYQW"
     gene            2143..>2280
                     /locus_tag="WIX60_001957"
     CDS             2143..>2280
                     /locus_tag="WIX60_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ5306167.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YL"
BASE COUNT          705 a          562 c          559 g          454 t
ORIGIN      
        1 ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg gcacggaaac ttaccgaata
       61 aaacagtttc cttagattcc acgtcccaga ttcccgcctt cgcgggaatg acgagatttt
      121 aagttggggg aatttatcag aaaaccccca acccccaaaa accgggcgga tgccgcacca
      181 tccgccccca aaccccgatt taaccattca aacaaaccaa aagaaaaaac aaaatgaaca
      241 aaatataccg catcatttgg aatagtgccc tcaatgcctg ggtcgtcgta tccgagctca
      301 cacgcaacca caccaaacgc gcctccgcaa ccgtgaagac cgccgtattg gcgacactgt
      361 tgtttgcaac ggttcaggca agtgctaaca atgaagagca aggagaagat ttatatttag
      421 accccgtaca acgcactgtt gccgtgttga tagtcaattc cgataaagaa ggcacgggag
      481 aaaaagaaaa agtagaagaa aattcagatt gggcagtata tttcaacgag aaaggagtac
      541 taacagccag agaaatcacc ctcaaagccg gcgacaacct gaaaatcaaa caaaacggca
      601 caaacttcac ctactcgctg aaaaaagacc tcacagatct gaccagtgtt ggaactgaaa
      661 aattatcgtt tagcgcaaac ggcaataaag tcaacatcac aagcgacacc aaaggcttga
      721 attttgcgaa agaaacggct gggacgaacg gcgacaccac ggttcatctg aacggtattg
      781 gttcgacttt gaccgatacg ctgctgaata ccggagcgac cacaaacgta accaacgaca
      841 acgttaccga tgacgagaaa aaacgtgcgg caagcgttaa agacgtatta aacgctggct
      901 ggaacattaa aggcgttaaa cccggtacaa cagcttccga taacgttgat ttcgtccgca
      961 cttacgacac agtcgagttc ttgagcgcag atacgaaaac aacgactgtt aatgtggaaa
     1021 gcaaagacaa cggcaagaaa accgaagtta aaatcggtgc gaagacttct gttattaaag
     1081 aaaaagacgg taagttggtt actggtaaag acaaaggcga gaatggttct tctacagacg
     1141 aaggcgaagg cttagtgact gcaaaagaag tgattgatgc agtaaacaag gctggttgga
     1201 gaatgaaaac aacaaccgct aatggtcaaa caggtcaagc tgacaagttt gaaaccgtta
     1261 catcaggcac aaatgtaacc tttgctagtg gtaaaggtac aactgcgact gtaagtaaag
     1321 atgatcaagg caacatcact gttatgtatg atgtaaatgt cggcgatgcc ctaaacgtca
     1381 atcagctgca aaacagcggt tggaatttgg attccaaagc ggttgcaggt tcttcgggca
     1441 aagtcatcag cggcaatgtt tcgccgagca agggaaagat ggatgaaacc gtcaacatta
     1501 atgccggcaa caacatcgag attacccgca acggtaaaaa tatcgacatc gccacttcga
     1561 tgaccccgca gttttccagc gtttcgctcg gcgcgggggc ggatgcgccc actttgagcg
     1621 tggatgggga cgcattgaat gtcggcagca agaaggacaa caaacccgtc cgcattacca
     1681 atgtcgcccc gggcgttaaa gagggggatg ttacaaacgt cgcacaactt aaaggcgtgg
     1741 cgcaaaactt gaacaaccgc atcgacaatg tggacggcaa cgcgcgtgcg ggcatcgccc
     1801 aagcgattgc aaccgcaggt ctggttcagg cgtatttgcc cggcaagagt atgatggcga
     1861 tcggcggcgg cacttatcgc ggcgaagccg gttacgccat cggctactcc agtatttccg
     1921 acggcggaaa ttggattatc aaaggcacgg cttccggcaa ttcgcgcggc catttcggtg
     1981 cttccgcatc tgtcggttat cagtggtaag ggctttttcg cctgtctgct gttgggacag
     2041 gcggaaggtt tgaagggaag ggtggcgatt tgccgcctga gacctttgca aaatcccccc
     2101 aaaatcccct aaattcccac caagacattt aggggatttc tcatgagcac cttcttccgg
     2161 caaaccgcgc aagccatgat tgccaaacac atcaaccgtt tcccgctatt gaagttggac
     2221 caagtgattg attggcagcc gatcgagcag tacctgaacc gtcaaaaaac ccgttacctc
//
LOCUS       DAWIVB010000218         2261 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWIVB010000218 DAWIVB010000000
VERSION     DAWIVB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.218"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..567)
                     /locus_tag="WIX60_001958"
     CDS             complement(<1..567)
                     /locus_tag="WIX60_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ5306168.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAK"
     gene            730..2136
                     /gene="dnaB"
                     /locus_tag="WIX60_001959"
     CDS             730..2136
                     /gene="dnaB"
                     /locus_tag="WIX60_001959"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ5306169.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          544 a          585 c          607 g          525 t
ORIGIN      
        1 tttggcgact tcgtcccagt tgacgatttc ccagaaacct ttcaggtagt tgggacggct
       61 gttgcggtag tcgatgtaat aggcgtgttc ccacacgtcg caggtcagca gcggcgtgtt
      121 ttcagtggtc agcggagttg ctgcgttgga agtggaaacc aaatccaatc cgccggcagg
      181 ggttttcacc agccacgccc aaccggagcc gaacgtaccg gccgcgcagg cattgaacgc
      241 ttcttggaat ttctcgaagc tgccccattt cgcgtcgatg gcggcggcca gttcaccgga
      301 aggtttgcct tgacctttgg gcgtgaaacc cagccagtag aaagtgtggt tccaagtttg
      361 ggctgcgttg ttgaacacgc cgcccgaaga ttttttcaca atctcttcca aaggcaggtt
      421 ttcaaattcg gtgcctttga tttgattgtt caggttggtg atgtaggttt gatggtgttt
      481 gccgtagtgg aattccaaag tctctttgct cagatgcggg gacaatgcgt ccagttcata
      541 aggcagttgc ggcagcttat gttccatttt gtgctcctaa tattgtttta aatgttgtat
      601 tttggcagtg ttgctgcaaa tagcttggca gcgcacatat tttacctgtt ttgaggaatg
      661 gaaaccaatt aaacctgctt tacgctataa tagaagattg caatttcggc acgacagata
      721 ggatgtacca tgaacgatta cgcagccatg ccgtctgaag accgtgaggt cggcgcattg
      781 tcgcttccgc cgcactcaat ggaggcggaa caatccgttt tgggcgggtt gatgctggaa
      841 aatccggcat gggacaggat tgccgatgtg gtttcgggag aggatttcta ccggcatgaa
      901 caccgcctga ttttccgttc cattgccaaa ttaatcaatg agagccgtcc cgcagatgtg
      961 attacggttc aggaagattt gcagcggaac gaagaattgg aagcggcagg cggattcgaa
     1021 tacctgatta cgctggcgca aaacaccccg tctgccgcca acatccgccg ctacgccgaa
     1081 atcgtgcgcg agcgttccat catgcgccaa ctcgccgaag tggggacgga aatcgcccgc
     1141 agcgcataca atccgcaagg cagggacgcg gggcagcttt tggacgaggc ggaaaacaaa
     1201 gtattccaaa tcgccgaaag cacggccaaa tccaagcagg gctttttgga gatgcccgat
     1261 ttgctgaaag aagtcgtaca gcgcatcgat atgctctact cgcgcgacaa tcccgatgaa
     1321 gttaccggcg tgccgacggg gttcatcgac ctcgacaaaa aaacctcggg tctgcaaccc
     1381 ggcgacctga ttatcgttgc cggtcgtccg tctatgggta agaccgcctt ttctatcaat
     1441 atcgccgaac acgttgccgt agaaggcaag ctgcccgttg ccgtcttttc gatggagatg
     1501 ggcggtgcgc aactggtcat gcgtatgctc ggctcggtcg gacggttgga tcaaagcgtc
     1561 ctgaaaacag gcaggctcga agacgaacac tggggtcgtc tgaatgaagc agtcgtcaaa
     1621 ctctcagacg cgcccgtgta catcgacgag accccgggtc tgactgcgct cgaactgcgc
     1681 gcccgtgccc gccgtctcgc ccgtcaattt aacaataagc tgggattaat cgtcatcgac
     1741 tacctgcaac tgatggcggg atccggccgt tccgacaacc gtgcttcgga gcttggcgaa
     1801 atttcccgct ccctcaaagc cctggcaaaa gagttgcaag tccccatcat cgccctgtcg
     1861 caactgagcc gcacggtcga atcgcgtacc gacaaacgcc cgatgatgtc cgaccttcgc
     1921 gagtccggcg caatcgagca ggatgccgac ctgattatgt tcatgtatcg cgacgaatac
     1981 tacaaccaag actcacccat gaaaggcctt gccgaatgta tcatcggcaa acaccgcaac
     2041 ggtcccgtcg gtaaaatctt cctcacatgg acgggacaat tcaccaaatt cgacaatgct
     2101 gcctatattc ccgaggaggc aaagatagag gattaaatgg ctatataaaa atttattagg
     2161 cgaaatcagg caaaatcgtt taaaatcatg ctgagagatt gccctaaaaa ataaaacgcg
     2221 gtcttgaggc atttttgcat tcagcccgca tataattgaa a
//
LOCUS       DAWIVB010000219         2207 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWIVB010000219 DAWIVB010000000
VERSION     DAWIVB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.219"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            32..516
                     /locus_tag="WIX60_001960"
                     /pseudo
     CDS             32..516
                     /locus_tag="WIX60_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            612..1235
                     /gene="rsmG"
                     /locus_tag="WIX60_001961"
     CDS             612..1235
                     /gene="rsmG"
                     /locus_tag="WIX60_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ5306170.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLVYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1334..2107
                     /locus_tag="WIX60_001962"
     CDS             1334..2107
                     /locus_tag="WIX60_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ5306171.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVEEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
BASE COUNT          432 a          505 c          768 g          502 t
ORIGIN      
        1 atgcggtctg cttcggaaga gggggacggc gatgtttgtg aacgagaaat atccttatgc
       61 ggctctgttt gcgggactgg tgtttttgac gctgccgttt gcgttggcgg tgcatgatgc
      121 ctttgcgctt gcgttcggac ggacggggtt gctggtgtcg gtgtcggacg gcggattcgg
      181 ctggcgtggc ggttgggacg gcactgtttg gtttgtgttc ggtgtgtttg cgtttttgaa
      241 tgtggttgtg tcggcgggtc tgacgaaact ggcgtacaaa aagatgatgc ggcggcattc
      301 gcgttacaca ctgtttctgt cgggcgtggc ggcttgcgcg gcggcagcgg tggcttggat
      361 tttcgagctg ctgcttggca gtggggcttt gggcgggctg cgggggaggc ggtgttggaa
      421 tatgcgtttg ccgtgtggct ggtggcgatg ctgacgctgc ccaaacgcct gacgcgcgcg
      481 ccggtgcagc cggtggtgtt tcacaggaaa aaataggttg gaacgggaaa tgccgtctga
      541 aacccgacac gcggtttcag acggcatgtt tttccgctaa cattacgcct gaatatggac
      601 aggaagcaga tatggaacgc aaagaacgcc tgcgtgcagg cattgccgcg atggggctgg
      661 atatttcgga aacggcgcag gacaggcttt tggtctatgt ggatttgttg aaaaagtgga
      721 acaaaaccta caatctgacc gccctgcgcg acgaggaaaa aatgattgtc catcatcttt
      781 tggacagcct gacgctgctg ccccatatcg agggtgtgca aacgatgctg gatgtcggtt
      841 cgggcggcgg tcagcccggc attccggcgg cggtgtgccg tccggatgtg caaataaccc
      901 ttttggatgc gaatacgaag aaaacggctt ttttacagca ggcggttatc gagttggggt
      961 tggacaatgt gcgcgtggta tccggacgcg tggaggcggt ttcggacgtg cgtgccgatg
     1021 tggttaccag ccgtgcgttt gcagaactgg cggattttgt gtcgtggacg gtgcatctgt
     1081 tgaaagacgg cggctactgg gcggcgatga agggcgtgta tccgcaggaa gaaatcggcc
     1141 gcctgccgca ggatgtgtgc gttgaaaaag tccaaaggct cgacgtgccg ggcttggatg
     1201 cggaacgcca tatcgtcatc ctgagcaagc gttgagcgca cttcagacgg catgaatacc
     1261 ttttttgtgc ggataaaggt aaaattccgc actgtttttc ttttttcaac atcagacggg
     1321 acacgggcgg gacatgagtg cgaacatcct tgccatcgcc aatcagaagg gcggtgtggg
     1381 caaaacgacg acgacggtaa atttggcggc ttcgctggca tcgcgcggca aacgcgtgct
     1441 ggtggtcgat ttggatccgc agggcaatgc gacgacgggc agcggcatcg acaaggcggg
     1501 tttgcagtcc ggcgtttatc aggtcttatt gggcgatgcg gacgtgcagt cggcggcggt
     1561 acgcagcaaa gagggcggat acgctgtgtt gggtgcgaac cgcgcgctgg ccggcgcgga
     1621 aatcgaactg gtgcaggaaa tcgcccggga agtgcgtttg aaaaacgcgc tcaaggcagt
     1681 ggaagaagat tacgacttta tcctgatcga ctgcccgcct tcgctgacgc tgttgacgct
     1741 taacgggctg gtggcggcgg gcggcgtgat tgtgccgatg ttgtgcgaat attacgcgct
     1801 ggaagggatt tccgatttga ttgcgaccgt gcgcaaaatc cgtcaggcgg tcaatcccga
     1861 tttggacatc acgggcatcg tgcgcacgat gtacgacagc cgcagcaggc tggttgccga
     1921 agtcagcgaa cagttgcgca gccatttcgg ggatttgctt tttgaaaccg tcatcccgcg
     1981 caatatccgc cttgcggaag cgccgagcca cggtatgccg gtgatggctt acgacgcgca
     2041 ggcaaagggt accaaggcgt atcttgcctt ggcggacgag ctggcggcga gggtgtcggg
     2101 gaaataggtc aatccaaatc gggctgcccg tgcctttatg ctgtttggcc gggtgcgtta
     2161 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagt
//
LOCUS       DAWIVB010000220         2144 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWIVB010000220 DAWIVB010000000
VERSION     DAWIVB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.220"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            576..>2144
                     /locus_tag="WIX60_001963"
     CDS             576..>2144
                     /locus_tag="WIX60_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ5306172.1"
                     /translation="MNEQNQLKVKQKDSYSTLREKDRERKFIYNKGSQGGGSVFFDNT
                     DTLVSRQSGTAVFGTATYLPPYGKVSGFDADGLNKRGNAAGWIRTTRIALAGYSYEGV
                     VCRSGTGCPKLVYKTRFSFDNPDLVKNAGRLDRHTDPSRENSPIYKLKDYPWLGVSFN
                     LGAEGTTKDGKTINKLVSSFDEKNSSNNNLVYTTEGRDISLGDWQREKTAMAYYLNAK
                     LHLLDKKGIKDITNKTVQLGVLRPSIDVRLQRNTGLAGLLNFWASWDIKDNGQIPVKL
                     GLPEVKAGRCINANNPNKSTKAPSPALTAPALWFGPVQNGKMEMYSASVSTYPDSSSS
                     RIFLQNLKRKNDPNKPGRYSLATLNKSDIESREPTFTGRQTVIRLDKGVHQIKLKGNE
                     VEGFKGNNGNDTFGIVSEGSFMPDDSEWKKVLLPWTVRGVNDDQFKTFNKEEKNGKPK
                     YSQKYRSRDNGNNSKRDLGDIVNSPIVAVGGYLATSANDGMVHIFKKGNGDARNYSLK
                     LSYIPGTMPRKDIES"
BASE COUNT          594 a          546 c          561 g          443 t
ORIGIN      
        1 ataaaacggt ttttttgaga ttttacgttc cggattcccg ctttcgcggc aatggcgggc
       61 caaggaggcg gttgcggatt gccgaaaacc gctttaccat gatcagcgcg caagggtaga
      121 ataatgcggt aaccctatac attgcatccg gccaaagggg cgtgttactt ttccgaacat
      181 ccccctttgg cagccgggcc aaaggggttt tccaaccagc aattcggcgg gcgcggaatc
      241 gggcggtttg ccgaatcccg ccgtttgcgg cgcgcctgcc gccgacggta tcccgcgaag
      301 caagatttaa gggataaaat atgttccaac acgcagggcg gcacataagg cgccgccctg
      361 attcggaagg gcttgcaccc ctcccggaca aagcctgatc ctgccgcccc gaaggacgga
      421 tgcccgaagg gcggggggtt tgaccgaaaa ggaaatacga tgaataaaac tttaaaaagg
      481 caggttttcc gccataccgc gctttatgcc gccatcttga tgttttccca taccgggggg
      541 gggcgcaggc ggaaacccat aactacgcta ttgtcatgaa cgaacaaaat cagctgaagg
      601 taaagcagaa ggattcatat tcaaccttaa gggaaaaaga cagggaacgc aaatttatct
      661 ataataaagg ctcgcagggt ggaggctctg tctttttcga caataccgat acccttgttt
      721 cccgacaaag cggtactgcc gtttttggca cagccaccta cctgccgccc tacggcaagg
      781 tttccggttt tgatgccgac gggctgaaca agcgcggcaa tgccgccggt tggattcgta
      841 ccacccgcat cgcgctggca ggctacagct acgaaggtgt cgtatgcaga agcggcacag
      901 gctgtcccaa acttgtctat aaaacccgat tttccttcga taatcccgac ttggtaaaaa
      961 atgcgggcag gctggatagg cacacagacc caagccgcga aaattcgccc atttacaaat
     1021 tgaaggatta tccatggttg ggcgtatctt tcaatttggg tgccgagggt accaccaaag
     1081 atggtaagac aatcaacaaa ttggtatctt cttttgatga aaagaatagt agtaataaca
     1141 acctcgtcta taccacggaa ggccgcgata tttccttggg cgactggcag cgcgaaaaga
     1201 ccgccatggc ctattatctg aacgccaaac tgcacctgct ggacaaaaaa gggattaaag
     1261 atatcaccaa caaaacagtg cagttgggtg tcttgaggcc gagcatcgat gtgcggctgc
     1321 aaagaaatac ggggcttgcc ggcttgctaa atttttgggc tagttgggac attaaagata
     1381 acgggcagat tccggtcaag ctcggcctgc cggaagtcaa agcaggccgc tgcatcaacg
     1441 caaataaccc caataagagt accaaagccc cttcccccgc actgactgcc cccgcgctgt
     1501 ggttcggacc tgtgcaaaat ggtaagatgg agatgtattc cgcttcggtt tctacctacc
     1561 ccgacagttc gagcagccgc atcttccttc aaaatctgaa aagaaaaaac gaccccaaca
     1621 aacccggccg ctattccctc gcaaccttga ataagtcgga tattgaaagt cgagagccga
     1681 ctttcacagg gcggcaaacc gtcatccgat tggataaagg cgtacatcag atcaaactta
     1741 aaggcaatga ggtcgaaggt tttaagggaa acaacggcaa cgacactttc ggcattgtta
     1801 gtgaagggag cttcatgcct gatgacagcg agtggaaaaa agtgctgctg ccttggacgg
     1861 ttcggggtgt caatgacgat caatttaaaa cattcaacaa agaagaaaaa aacggcaagc
     1921 caaaatacag tcaaaaatac cgcagccgcg acaacggcaa caatagcaag cgcgatttgg
     1981 gcgacatcgt caacagcccc atcgtggcgg tcggcgggta tttggctact tccgccaacg
     2041 acgggatggt gcatatcttc aaaaaaggca acggggacgc gcgcaactat agtctgaagc
     2101 tcagttatat cccgggcacg atgccgcgca aggatattga aagc
//
LOCUS       DAWIVB010000221         2137 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWIVB010000221 DAWIVB010000000
VERSION     DAWIVB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.221"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            116..1996
                     /locus_tag="WIX60_001964"
     CDS             116..1996
                     /locus_tag="WIX60_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ5306173.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNIATNLRIQAIEPHFNAETGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          572 a          661 c          507 g          397 t
ORIGIN      
        1 actataacgc aagcaccgca aagccgcgcc aaccctctcc caaccttttt tcagacggca
       61 ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac agaaaaatcc gaaaaatgaa
      121 tattttttac gaagagtccg gccaattcaa aatcgccgcc atcatccaaa aaaacgatgc
      181 cacctaccaa gtcgataccc cacacggcaa acgcaccaaa gtgaaggcga acaacgtctt
      241 tgccgagttt gacggcgata tggcggcgtt tttggaaaac gcgcaggcac aggcggcgga
      301 catcgacacc gatttattgt gggaagtatg cggcgaagag gaatttaccg ccgaagccat
      361 tgccgaagaa tattacggcc atgcgccgac caaaaccgag ctggcggcaa ctttgattgc
      421 gctttacgcc gcgccgatgt atttctacaa aaaagccaaa ggcgtgttca aagccgcgcc
      481 cgaagaaact ttaaaacaag cacttgccgc catcgaacgc aaaaaacagc aagacgcgca
      541 aatcgacgct tgggcagaag ccttgaaacg cggcgagatg ccgtctgaaa tcgcggcgga
      601 tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg acctacaaag cctttaccaa
      661 agccgccgac gcgctgaaaa cctctgccta cgaattggcg aaaaaaacgg gcggcattac
      721 gtccattccc caatacctgc aagacgggtt tgaaatcaaa tacttcccta aaggaacagg
      781 cttccccgac cttgcccttc ccgaaatgcc cgacctgccc aaggccgacg ttaccgcctt
      841 ttccattgac gacgaatcaa ccaccgaagt ggacgacgct ttaagcctga ccgacttgga
      901 caacggcacg aagcgtgtcg gcatccacat cgccgcgccg tcacttgccg ttaaaccggg
      961 cgacaaaatg gaaaaaaaca tcatggaacg cttgagcacg gtttatttcc ccggcggcaa
     1021 aatcacgatg ctgcccgaaa actggattgc cgcgttcagc cttgatgcag gcgcacaccg
     1081 ccctgccgtc agcatttatt tcgatgtgga cggcgagttc aacgtcggcg cgccgacctg
     1141 caaaatcgaa gcggtcaaca tcgccacaaa cctgcgtatc caagccatcg agccgcattt
     1201 caacgccgaa accggcttgg acgaagccgg cgaaatgatg ttcgcccacc atcaagacct
     1261 gatttggttc tatcaattcg ccaccgccct gcaaaaagcg cgcggcaaat acgaacccga
     1321 ccgcgcgccg caatacgatt acagcatcga attggatgag gaaggcaagg tatccgtcgt
     1381 ccgccgcgaa cgcggctcac ccatcgatac gctggtcagc gagatgatga ttcttgccaa
     1441 cagcacttgg gcacaaatgc tccatgacaa cgacctgccc ggcctcttcc gcgtccaacc
     1501 caccggaaaa gtacgcatga gcaccaaatc cgagccgcat atcggcatgg gcgtgcagca
     1561 ttacggctgg tttacctcgc cgctgcgccg cgccgccgac tatatcaacc aaaagcagct
     1621 gatcagcctg atagacgaca ctgccgagcc gctgtatcaa aacagcgatg ccgagctttt
     1681 cgccgcactg cgcgactttg atgccgccta taccgcctac gccgatttcc aacggcagat
     1741 ggaagcctac tggagccttg tgtacctgca acagcaaggt acaagcgagc tgaccgcgac
     1801 catcctcaaa gaagacctcg tccgcatcga aggcctgccg ctggtcacgc gcgcgaccgg
     1861 tattccgttt gacgcactgc ccaaatcaca ggcattgttc aaaatcaccg aattggatgc
     1921 cgagaagcag tttgtctcgc tcaactacat caaggcagtc gcacccgcgg gtacaacggc
     1981 aggcaatgcc gtctgaagcc cgatacggca aactccggca aaaacagaaa cctgaatctc
     2041 atcattccca cagtcgggaa tccggttttt tgggtttccg ttgtttttcc gtttcaatga
     2101 acttccaagc cgccgttccc gataaatacc cgcaatc
//
LOCUS       DAWIVB010000222         2129 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWIVB010000222 DAWIVB010000000
VERSION     DAWIVB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.222"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            324..836
                     /locus_tag="WIX60_001965"
     CDS             324..836
                     /locus_tag="WIX60_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ5306174.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            916..1995
                     /gene="aroB"
                     /locus_tag="WIX60_001966"
     CDS             916..1995
                     /gene="aroB"
                     /locus_tag="WIX60_001966"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ5306175.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          513 a          667 c          521 g          428 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatagtag tgataaacat attatcttca atatattcaa
       61 ttggataatt gtttacctaa gtaaagataa ttgccttttc ctgacaaata agtgaaatca
      121 acggattgtc aaaacacagc ctgaaataaa aaacctccct gatttctttt atttttcctt
      181 aaaatcagaa aggttcggga tggtcgggtt atttttccaa acgtaccgct gccctgccga
      241 tttcgtataa aattccgccg taacccgaca agcccgaacc ctgtcgcccc gaaaggcggg
      301 gtgtcaaaca ttaaggaatt gtgatgaaaa actttaacgg caaactcatc ctcatcggac
      361 tgatgggcgc gggcaaaacc acgctgggcc ggcaaatggc gcagcggctg gattaccgtt
      421 tttacgacag cgatcacgaa atcgccgcag ccgccggcgt tcccatcccc accatatttg
      481 aaatggaagg cgaacaggga ttccgttcgc gcgaaaccgc catactcaaa aagctggtta
      541 tcctgcccca tatcgtcctg tccaccggcg gcggcgcggt gttaaaagaa gaaaaccgcg
      601 cccttatccg caaaagcggc acggtcgtct atctgcacgc cccgcccgaa accctgctcg
      661 aacgcacgcg ctgcgacaac agccgtcctt tgctgcaagt tgccgatcct ttggcgaaat
      721 tacgtgaact ctacgccgca cgcgaccccg tttaccgcca aaccgccgac tttaccgtag
      781 aatcggcaaa ctgccgggaa accgtgcaaa ccctgctcaa acgcttatcc cgataaaccg
      841 gcatatgcgc cgcgcccaga aaaccaagcc gcgccccgcc cggcgggccg gcggttcaaa
      901 ctttaaggaa caacaatgaa aacactgacc gtacacaccc cgtcccacag ctaccccatc
      961 tttatcggca acggactgct gccgcaggca ggaagcctgc tcaaaccgca tttgggcaaa
     1021 cgcgccgcca tcatcgccaa cgaaaccgtc gccccgctct acctcggcac gcttcagacg
     1081 gcattggatg cggcaggcgt atcccatttc agcatcatcc tgcccgacgg cgaggcgcac
     1141 aaaaactggc agacgctcaa cctcatcttt gacgggctga tgcaaaaccg cgccgaacgc
     1201 aaaaccacat taatcgcact gggcggcggc gtgatcggcg acatggtcgg ctttgccgct
     1261 gccacctacc agcgcggcgc accgttcgtt caaataccga ccacgctgtt gagtcaggtc
     1321 gactcatcgg tgggcggcaa aaccgccatc aaccacccgc tcggcaaaaa tatgattggc
     1381 gcgttttacc agccgcaggc ggtgcttgcc gacttggaca cgctgcacac cctgcccgcc
     1441 cgcgaattgt ccgccggtat ggcggaagtc atcaaatacg gcgcgctcgg cgacatcggc
     1501 ttttttgaat ggctggaaca gcatatgccc gaactgatga cgctcgatcg ggaaaaactc
     1561 gcccaagccg tgtaccgctg ctgccaaatg aaggcagaca tcgtcgccca agacgaaacc
     1621 gaacagggca tacgcgcatg gctcaacctc ggacacacct tcggacacgc cattgaaacc
     1681 gagatgggtt acggcacttg gctgcatgga gaagccatcg ccgccggctg cgtgttggcg
     1741 gcgcgtttgt ccgaacaact gggcaaaacc tccgccgcag ataccgcgcg gctcgccgcc
     1801 ctgctcgaag ccgccggact gccgtccgcg ccacccgtgt ttgcctttga aaaatggctg
     1861 gaacacatga gccacgataa aaaagtcagc ggcggcatca tgcgctttat cggtctgaac
     1921 cggctgggcg aagccaacat caccgaaatt accgacacgg acatcctccg ccgcaccctg
     1981 caaccgtatc tctgatttcc tctgccgatg tgctgccgcg cgggtttgac gcacgatgat
     2041 gattttccat catctttctc cgcaaaagcg ggaatccagt ccgttcggtt tcggtcgttt
     2101 ccgataagtt cccgttgctt ttcatttct
//
LOCUS       DAWIVB010000223         2122 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWIVB010000223 DAWIVB010000000
VERSION     DAWIVB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.223"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            123..1508
                     /locus_tag="WIX60_001967"
     CDS             123..1508
                     /locus_tag="WIX60_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ5306176.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDILVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1528..2046
                     /locus_tag="WIX60_001968"
     CDS             1528..2046
                     /locus_tag="WIX60_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ5306177.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          446 a          646 c          571 g          459 t
ORIGIN      
        1 ttcaaataaa caggaatata acttcagaca aacaacttac cgcccgattt gtgctatcgt
       61 tttcgcacaa cttaaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acatactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttctg gtcggcctgg
      361 gcgcgatgct cggacgtttg gtcgaaacat ccggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga acctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcgacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgccgccgc gctgatggct cctgccgttg ccgccgccgg ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgt ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttcgcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccctat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgcaacag
     1741 gcgcaaaacg gtgcgaacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gccttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggggtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggattaca tt
//
LOCUS       DAWIVB010000224         2064 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWIVB010000224 DAWIVB010000000
VERSION     DAWIVB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.224"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..941
                     /locus_tag="WIX60_001969"
     CDS             <1..941
                     /locus_tag="WIX60_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEZ5306178.1"
                     /translation="NFHTNRNTAYKALKDACNNLFERQFSFIEKTPKGEKVVRTRWVS
                     QVAYIEQQATVELVFAPNVAPLITMLEKNFTSYELDQVSSLSSKYAVRLYEIIISWRA
                     AGKTPMFSTMELRERLGMMPDEYQKMELFKRKVLDFAVKQINDKTDISITYEQHKEGR
                     KIVGFTFSILHKIGSKDIPLENQSELFAGMTDLEAGTIRVRAEAYIASLIAKGQNVTK
                     AHRLNILKKAVEERWGFEDVAKDNGAKNPENKNAKVVLNEWEKISNGTRFKDKDGTIW
                     VKDSGMLRTEGTNRWIADSQIAKLFPMLTVMVEEGI"
     gene            1038..1454
                     /locus_tag="WIX60_001970"
                     /pseudo
     CDS             1038..1454
                     /locus_tag="WIX60_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(1460..1558)
                     /locus_tag="WIX60_001971"
                     /pseudo
     CDS             complement(1460..1558)
                     /locus_tag="WIX60_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1651..>2064
                     /locus_tag="WIX60_001972"
     CDS             1651..>2064
                     /locus_tag="WIX60_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306179.1"
                     /translation="MNRTLYKVVFNKHRNCMIAVAENAKREGKNTADTQAVGILPNDI
                     AGFAGFIHSISVISFSLSLLLGSALILTSSSATAQGIVADKSAPAQQQPTILQTGNGI
                     PQVNIQTPTSAGVSVNQYAQFDVGNRGAILNNSRSN"
BASE COUNT          609 a          450 c          477 g          528 t
ORIGIN      
        1 ataatttcca taccaaccgt aatacggcgt ataaggcttt gaaagatgcc tgtaacaacc
       61 tttttgaacg ccaattcagt tttatcgaaa aaacaccaaa aggggaaaag gtagtacgga
      121 caaggtgggt atctcaagtc gcctatattg aacaacaggc aacggtagag ttagtttttg
      181 caccaaatgt tgcccctttg attacgatgc tagaaaaaaa cttcacaagc tacgagcttg
      241 atcaggtctc atcgttgagc agtaaatacg cggtgcggct ctacgaaatt attatttcat
      301 ggcgtgcagc cggtaagaca ccgatgttca gtacaatgga gttgcgcgaa cgtttgggta
      361 tgatgcctga cgagtatcaa aaaatggagc tattcaaacg taaggttttg gatttcgccg
      421 tcaagcagat caatgataaa acggatattt ccattaccta cgagcagcat aaagaaggac
      481 gaaaaattgt aggttttaca ttctcaatcc tacataaaat agggtcgaaa gacatccctc
      541 ttgaaaatca atcggaactt tttgccggaa tgactgattt ggaagccgga acgatacggg
      601 tcagggcgga agcatatatc gcttcgctca ttgcaaaagg tcagaacgtg actaaagccc
      661 ataggctgaa tattctgaaa aaagccgtag aggaacgttg gggatttgaa gatgtggcta
      721 aagataatgg ggccaaaaat cctgaaaaca aaaatgcaaa agtcgtttta aacgaatggg
      781 aaaagatttc taatggcaca cgttttaagg acaaggatgg aacaatttgg gttaaagatt
      841 caggtatgct taggactgaa gggacaaaca ggtggatagc cgattctcag attgccaaat
      901 tatttcctat gttaactgtg atggttgagg aaggtattta acccggcaac tcgcgtattc
      961 tacctgtttt gcggtacgga aaccaattga acctgcttta cgctacaata gaagattgca
     1021 attttgtcgg atgtaccatg aacgattaca cagccatgcc gtctgaagac ggaggaatcg
     1081 gctcattatc gcttccgccg cactcaatgg aggcggaaca atccgttttg ggcgggttga
     1141 tgctggaaaa tccggcttgg gacaggattg ccgatgtggt ttcgggagag gatttctacc
     1201 gccacgaaca ccgcctgatt ttccgatcca ttgccaaact gatcaatgag agccgtcctg
     1261 ccgatgtcat cacggttcag gaagatttgc agcggaacga agagctggaa gcggcggggg
     1321 gattcgaata tctgattacg ctggcgcaaa atactccgtc tgccgccaac atccggcgcc
     1381 atgccgaaat cgtgcgcgag cgttccatta tgcgccaact cgccgaagtg ggaacggaaa
     1441 ttgcccgcag cgccataccc aaaatatcgg cagcggaacg ggcggtagct tggagtacga
     1501 aaaattcaag cagtttcttt tgaactctat tgtttaattt acaattggtt atctttattt
     1561 tcgtagcctg gcataactgt taatctacgt cagcccctaa aaattaatct aatttacgat
     1621 tataaaaata ttattaagaa agcccttact atgaaccgca ccctgtacaa agttgtattt
     1681 aacaaacatc gaaactgcat gatagccgtt gctgaaaatg ccaaacgcga gggcaaaaac
     1741 acagccgaca cccaagctgt aggtattttg ccaaatgata ttgcgggctt tgcgggtttt
     1801 atccattcta tctctgttat ctcattctcc ctttcattac tgctcggttc tgcccttatc
     1861 ctgacttctt cttctgctac tgcccaaggt atcgttgccg acaaatccgc acctgcacag
     1921 caacagccta ccatcctgca aacaggtaac ggcataccgc aagtcaatat tcaaacccct
     1981 acttcggcag gggtttctgt taatcaatac gcccagtttg atgtgggtaa tcgcggggcg
     2041 attttaaaca acagtcgcag caac
//
LOCUS       DAWIVB010000225         1993 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWIVB010000225 DAWIVB010000000
VERSION     DAWIVB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.225"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            254..>1993
                     /locus_tag="WIX60_001973"
     CDS             254..>1993
                     /locus_tag="WIX60_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ5306180.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGGQAQAQTYPYALVMN
                     GQQLPEVKWGNSYNQLTHKNNTRQATFHSYFQGTRKKTTFSFNNTDEVVAQKNGVTVF
                     GAATYLPPYGKVSGFDTVKLTERANALNWINTTRPGLVGYHYQDSTCSSGNCPELSYK
                     TQFTFGHSSLAKKTNGGKLDIYEDKSRDGSPIYKLQGYSWLGVSFNLSGESTAESKKL
                     KNLVSSFSEEVTQNNGADSQHKDKNLVYTTGDGRNNNKTHQDKHHAVAFYLNAKLHLL
                     DKKQIKNITQGSELNLGVLKTRIEPTDAWKNKRHLVINADWEFKDTGTVSVKLKLPEV
                     KAGRCINKPNPNNNTKVPSPALTAPALWFGPVKDGKAEMYSASVSTYPDSSSSRIFLQ
                     NLKRKDDPNKPGRHSLATLTENDIKNREPNFTGRQTIIRLDGGVQQIKLGRNNDEVVN
                     FNGNNGNNATFGIVKDLGVDPDANEWKKVLLPWTVRAFNDDGRFNTVNKEENNGKPKY
                     SQRYRIRENGNNGKRNLGDIVNSPIVAVGEYLATSANDGMVHIFKQSGGDERNYSLKL
                     SYIPGTMPRKDIQS"
BASE COUNT          602 a          500 c          478 g          413 t
ORIGIN      
        1 acgtaaaatc taaagaaacc gtgttgtaac ggcagaccga tgccgtcatt cccgcgtagg
       61 cgggaatcta gactgtcggg catctgcagc ggtttgctaa aaaacgcttt accgtgatca
      121 gtgtgcaaag ttaaaatggg gaggtaagct tttcaatcag caattcggcg ggcgcggaat
      181 cgggcggttt accgaacccc ggcgttcgcg gcgcgccgcc ccgtcccgtg aaggcaaact
      241 caaggaataa aagatgaata aaacttggaa acggcaggtt ttccgccata ccgcgcttta
      301 taccgccata ttgatgtttt cccataccgg cggcgggggg gggcaggcgc aagcgcaaac
      361 gtatccatac gctcttgtaa tgaacggaca acagctgcct gaggtaaagt gggggaattc
      421 atataatcaa ttgacgcaca aaaacaatac ccgccaagct acctttcatt catattttca
      481 gggaactaga aaaaaaacta ctttctcatt caataatacc gatgaagttg ttgctcaaaa
      541 aaacggtgtt accgttttcg gcgcggcgac ctacctgccg ccctacggca aggtttccgg
      601 ttttgatacc gttaagctga ccgagcgcgc caatgccctt aattggatta atacgacccg
      661 cccggggttg gtaggctacc actaccaaga tagcacatgc tccagcggaa actgtcctga
      721 acttagctat aaaacccaat ttaccttcgg tcattccagt ttggcaaaaa agacaaacgg
      781 cggcaagctg gatatatacg aagacaaaag ccgcgacggt tcgcccattt acaaattgca
      841 aggttattct tggttgggcg tgtctttcaa tttgagcggc gagagcaccg ccgaatcaaa
      901 gaaactcaag aacttggtat cttcttttag cgaggaagta acgcagaaca atggtgcgga
      961 cagccaacac aaagacaaaa acctcgtcta tacgacaggc gatggccgta ataataataa
     1021 aacccatcag gacaaacacc acgccgtcgc cttttatctg aacgccaaac tgcacctgct
     1081 ggataaaaaa caaatcaaaa atatcacgca aggcagtgaa cttaatttgg gtgtcttgaa
     1141 aacgcgcatc gagccgacgg acgcgtggaa gaataagaga catctcgtta ttaatgctga
     1201 ttgggagttt aaagataccg gaacagtcag cgtcaaactc aaattgccgg aagtcaaagc
     1261 aggccgctgc atcaacaaac cgaaccccaa taataatacc aaagtccctt cgccggcact
     1321 gaccgccccc gcgctgtggt tcggacctgt gaaagatggt aaggcggaga tgtattccgc
     1381 ttcggtttct acctaccccg acagttcgag cagccgcatt ttccttcaaa atctgaaaag
     1441 aaaagacgac cccaacaaac ccggccgcca ttccctcgca accttgactg agaatgatat
     1501 taaaaatcga gagccgaatt tcacagggcg gcaaaccatc atccgattgg atggcggcgt
     1561 acagcagatc aaactgggta gaaacaatga tgaggtcgtc aattttaatg gaaacaacgg
     1621 caacaacgca actttcggca ttgttaagga cttgggcgtc gatcctgatg ccaatgagtg
     1681 gaaaaaagta ttgctgcctt ggacggttcg tgctttcaat gatgacggtc gatttaacac
     1741 agtcaacaaa gaagaaaaca acggcaagcc aaaatacagc caaagatacc gcatccgcga
     1801 aaacggcaac aacggcaagc gcaatttggg cgacatcgtc aacagcccca tcgtggcggt
     1861 cggcgaatat ctagctactt ccgccaacga cgggatggtg catatcttca aacaaagcgg
     1921 cggggacgag cgcaactata gtctgaagct cagttatatc cccggcacga tgccgcgcaa
     1981 ggatattcaa agc
//
LOCUS       DAWIVB010000226         1986 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWIVB010000226 DAWIVB010000000
VERSION     DAWIVB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.226"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            305..1762
                     /locus_tag="WIX60_001974"
     CDS             305..1762
                     /locus_tag="WIX60_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ5306181.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          452 a          592 c          457 g          485 t
ORIGIN      
        1 ctataatgcg cccttcggcg tggcggatat ataaggaagt gattttccat ctaagtaaaa
       61 accgccctat cggataagcc cttaacagaa aaggctttac ccgcgccgta tcggaacaca
      121 tcctctaaaa tacaatccgt tgaattgaaa aaaatataaa aacatccgcc cgcgaaaaac
      181 ggcagcgcgt cgtttgacaa agaatgaaaa tatcggttaa aaaccgattt tcatacaaaa
      241 aacaccgctg ccgtccgcat ccgtttcaga cggtattgag agaaaatctt ttaggagaac
      301 ctttatgtcc cggcatcccg cccccaccgg agaaaaaaca ttcttcggcc accccttcca
      361 gctttccacc ctcttccata tcgaattgtg ggaacgtttt tcattttacg gaatgcaggg
      421 catcctgctg atttacctct actacaccgc cgacaaaggc ggcttgggca tagacaaaac
      481 cctcgccggc ggcattgtcg gcgcatacag cggcagcgtg tacctgtcca ccattttggg
      541 ggcgtggttt gccgaccgag tatggggtgc ggaaaaaacc ctcttcctct cgggcatcgt
      601 cgtgatgctc ggacacatcg tccttgccgc cgccccgggc ctgtacggcc ttttaatcgg
      661 gctgatattc atcgcattgg gcagcggcgg cgtgaaatct acggccagtt ctatggtggg
      721 cgcattatac gaacaggacg aaatgcgccc gctgcgcgat gcgggatttt ccattttcta
      781 catcgccatc aacatcggcg gcttcctagg cccgctgctg accggcctac tgcaggaaaa
      841 catcggtttc cattatggtt tcggcgcggc ggcggtcggt atggcattcg gcttgtggcg
      901 ttattccctg ggacgtaaaa acctgcccca ccccaccgtc ccccatccgc tttcaaaagg
      961 acagggcaaa actgcggccg ccgtcggcat cgccctcatc gccgcacttg caaccgccat
     1021 caaaaccggg cttgtcaacc tcgacaattt ctccggcatc ctattatcta ccgtcatcct
     1081 tgccgtcatc gcctatttcg cccgcctgct gaccaacccc cgcgtcagtt ccgacaacaa
     1141 acggcacatc atcgcctaca tcccgctttt cctgaccatc tgtatgtttt gggccgtctg
     1201 gtttcagatt tacaccgtgg caaccgtcta tttcgacgaa accgtcaacc gcaccatcgg
     1261 ttcgtttacc gtgcccgtcg cttggaaaga ttctatgcaa agcctgtggg tcatcctgtt
     1321 ttccggactg atggcggcaa tgtggacaaa aatggggcgc aaacagccca aaaccccgct
     1381 gaaattcgct atggcggtat ttgttaccgg cgcgtcgttt ttgggattcg tcccctttat
     1441 ttcctccggt acgccgatgc ctattgcggt tttcgcactg atcgtcctcg ccatcacgat
     1501 aggcgaactg atgatttccc cgattgcgct gtccatctcc accaaaatcg caccgccttt
     1561 attcaaaacc caaatggtcg cccttaattt ccttgccttt tcattaggct tcactttggg
     1621 cggcgtattg tttgaaaaag gctatcaggc gggcgacgaa atcggcttct atcggctgct
     1681 gttctacatc ggcgcagcca caggcttcct gctgctcctg ctcgtcccca aattgaacaa
     1741 aatgctcgaa ggcacagact aagtcccgcc ccgatgccgt ctgaaccctt cagacggcat
     1801 ttttccgcat aatgaaacca aaccgtttcc acccgacagg acaggctccc gcccaaccgg
     1861 aaggcagcct gccgattgtc atttgaataa cgcaagggaa agccgttgat ttccgtttgt
     1921 atggaaacag tttggtttca ttggaaaaag gcattttgtc cgactaaatt agtgctgcat
     1981 caacga
//
LOCUS       DAWIVB010000227         1908 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWIVB010000227 DAWIVB010000000
VERSION     DAWIVB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.227"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(221..1039)
                     /gene="cysE"
                     /locus_tag="WIX60_001975"
     CDS             complement(221..1039)
                     /gene="cysE"
                     /locus_tag="WIX60_001975"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ5306182.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            1223..1801
                     /gene="grpE"
                     /locus_tag="WIX60_001976"
     CDS             1223..1801
                     /gene="grpE"
                     /locus_tag="WIX60_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ5306183.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
BASE COUNT          437 a          436 c          580 g          455 t
ORIGIN      
        1 tcgattccgt actatccgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
       61 ccactatatt tccggttgcg tgggaatcgg gtgtattgaa taaaaggcat tttgtccgac
      121 tggcaagtgc cgacatcggc ggcatatcaa ggcgcaggct tgaagcgggc aatgtcgtct
      181 gaagcccgtt tggcgtttca gacggcattg gtgcggatat tcaaatcata aagtcgattt
      241 cggtaaactg gatattttga tccatatccg ccgacggtgt tttgagcgat cgcgccacgg
      301 gtttggcggg tacgccgaca accgtgatgg acggcggcac gtctgaaacc acgacgctgc
      361 ccgccccgat tttggcattg ctgccgatgc ggatattgcc caatatcgag gcgtttgcgc
      421 cgatcatcac gccgtcgccg attttagggt ggcggtcgcc gccttctttg cccgaaccgc
      481 cgagcgttac gccgtgcaaa atcgaaatat tgttgcccaa cacggcggtt tcgccggcaa
      541 caaagccggt ggcgtggtcg agcatcagcc cgtatccgaa acgggcggcg ggatggatgt
      601 ccacgccgaa tacttcggac atacggtttt gcaggaaata cgccagcgtt ttgcgcccgt
      661 cgagatacag ccgatggttg atgcggtgtg cctgaatcgc gtggaagcct ttgaaatata
      721 aaagcggcag cgaatattcg tcgcaggcgg gatcgcgttc gtagatggct tttaagtctg
      781 cttcgacgca tttgccgatt tgggtgtcgc tgcccaacgc ctgctggtag atttcaaaca
      841 gcgcgcgcac gtccataatc gggctgccga gtttgctgga aaggtggtag gcaaggacgg
      901 agccgaggga ctcgtggcgc aacacggttt ggtgcaaaaa acttgccagc atcggttcgg
      961 cggagaccgc ggccgcggtt tcttcgcgga tggtgtgcca gaggtcgaaa ccggttgtgt
     1021 ttaaatggtc ttttttcatg agtgatgacg tttgaaaatc gatatggtcg gcagtatctt
     1081 accgtctata ttattttttc ggtaggggat ttgaaaatga atttgaaatt ctctgctttt
     1141 gcttgaagtt tcttgaaaat gtccttatct tgcgcgggta ataactggat tttgatttcc
     1201 aatttgtttt aagggatacg atatgagcga acagacacag cagcaaaaca gtgaagaagc
     1261 ggttgaaaat gtggaggcgg tggaaaccgt cgagacagta ggaaatgcgg acggtgtgca
     1321 ggaacaggct gccgcagagc cggcttatga ggatttgcag gcgcggattg ccgagctgga
     1381 agcgcagttg aaagacgagc agctgcgcgc tttggcaaac gagcaaaacc tgcgccgccg
     1441 ccaccagcag gaaattgcgg atacgcacaa gttcgccgga cagaagtttg ccgtggaaat
     1501 gctgccggtc aaggattatc tggaaatggc gcttttggat cagagcggca atttcgatgc
     1561 gctgaaaatg ggcgtgcaga tgactttgaa cgagttgcag aaagcatttg atgctacgca
     1621 aatcaaggaa atcaacccta aagcgggcga taagctcgat ccgaatatcc atcaggcgat
     1681 gcaggcggtg gcaagcgaac aggagccgaa taccgtggtg ggtgtgatga agaagggtta
     1741 tacgctgtcc gaccgcgtgt tgcgcccggc tatggttacg gtggcgcaga aggaagcctg
     1801 aaggcgtctg gggaataatc tgatttattt cctgaagcgc gttttgcgta taaaccgatc
     1861 gaagtaaagc ggcaatgccg tctgaacccg cctgtcgggc ttcagacg
//
LOCUS       DAWIVB010000228         1884 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWIVB010000228 DAWIVB010000000
VERSION     DAWIVB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.228"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(127..1437)
                     /locus_tag="WIX60_001977"
     CDS             complement(127..1437)
                     /locus_tag="WIX60_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ5306184.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYSA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
BASE COUNT          450 a          482 c          494 g          458 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggagta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcgttgcgca ggtggacggg
      361 cacttcgtcg ctagcgtttt ctttgacgaa gtggcgcatt tggttgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaagggttg
      961 caaaacatac acctgagcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaatacggc
     1201 ggaaacaggc aaaaactggg cgttgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcatcggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gatttaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta gaac
//
LOCUS       DAWIVB010000229         1871 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWIVB010000229 DAWIVB010000000
VERSION     DAWIVB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.229"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            226..1698
                     /gene="pyk"
                     /locus_tag="WIX60_001978"
     CDS             226..1698
                     /gene="pyk"
                     /locus_tag="WIX60_001978"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ5306185.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENALIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          477 a          543 c          508 g          343 t
ORIGIN      
        1 ttatctagga cagccccaaa ataaatttag cctaagtcat aaaaccgtgt aattttgcgc
       61 caaaaaaggc ggtttgcagg taaaatatca aactattaat ttaaatcata tttttacaga
      121 atattccgcc gcgttcatca aatggacatc aaaccggttc caaatttctg taattttgta
      181 acaaaatacc gcaaaacacc cgattgagac caaaaggact ttcatatgaa ccagacaagc
      241 cgcgatctga cccgcatcag ccacaacact aaaatcgtcg ccacccttgg gccgggcagc
      301 aacaacgtcg aactgttgga agacatgatc cgcgtcggcg gtctgaacgt cgtccgcttc
      361 aacttcagcc acggcacgcc cgaattccat caggaaaacg ccctcatcgt gcgcgaggcg
      421 gcaaaacgcg ccggacagga aatcgccatc attgccgacc tgcagggccc gaaaatccgc
      481 gtgggcaaaa tcgccggcgg cggcatcgaa ttgaacaaag gcgaaacgct ggtactggat
      541 gccgcgctcg aaggcgaagg cacgcgcgag gcggtcggtt tggactaccg cgacctgccc
      601 gacgacgttg ccgcaggcga tgtcttgtgg ctggacgacg gcctgctgac cctgaccgtg
      661 gaatccgtcg aaggcagcag gattatcaca agggtggaaa acagccacgt cctgaaaagc
      721 aacaagggca tcaacaaacg cggtggcggt ctgtccgcag gcgcgttgac cgaaaaagac
      781 ttccgcgacc tgaaaaccgc gattgccatc ggttgcgact acctcgccat cagctttgtg
      841 aaatccgccg aagatttgca tatcgcgcgc gccaaagtcg aagaagaaat gaagggcagc
      901 acggccgtgc gccccggttt ggtttccaaa atcgaacgcg tggaagcgat tgaaaacttg
      961 gacgaaatca tcctcgccgg cgacggcatt atggttgcgc gcggcgactt ggcggtcgaa
     1021 gtcggacacg ccgccgtccc cgccctgcaa aaacggatga tccgccgcgc ccgcgagttg
     1081 cgccgcttca gcattacggc gacgcaaatg atggaatcga tgattaccaa ccccgtaccg
     1141 acccgcgcgg aagtcagcga tgtggcaaac gcggtattgg acggtaccga tgcggtgatg
     1201 tgttccgccg aaaccgccgt cggcgcgtat ccgtttgaaa ccgtcagcca aatggcgatt
     1261 atctgcgcgg ctgcggaaaa agagcaggat tcgctcaacg gcgttgccga acaggtcgag
     1321 tatcccgaag cggtcagcac caacctggcg gttgccggcg gtgcggtcag cgtggcgcgc
     1381 gcggttcacg ccaaagccat cgtcgccctg accgaaagcg gttcgaccgc ctttgaaatc
     1441 agccgccaca acatcaccct gccgattttc gcgctgaccc cgagcgtttc cgcccaacgc
     1501 cgtatggcga tgtaccgggg cgtgcgcccg ctgattttgg caaccagtac cgaccacgac
     1561 acggcgttaa acgaagtcga aacgatgctg gtggaacaca acatcctgca ttccggcgac
     1621 caatacatca ttaccagcgg ttcgcaaatg cgcgaatccg gttcgaccaa cacactggaa
     1681 gtgctgcgcg tcaaataatc cgccctgagt ggaaaatgcc gtctgaagcc gatgcccgag
     1741 gcttcagacg gcattttttg cggcggcgcg gcggttcggg caaactctga aaaaattgtg
     1801 caaatccggc aacatcggat aaaatcgagt acctatacta aagcgaaaca aggcatttcc
     1861 gactgccttt t
//
LOCUS       DAWIVB010000230         1829 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWIVB010000230 DAWIVB010000000
VERSION     DAWIVB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.230"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            325..1770
                     /gene="zwf"
                     /locus_tag="WIX60_001979"
     CDS             325..1770
                     /gene="zwf"
                     /locus_tag="WIX60_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ5306186.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTAGFLAKAETNSKIHIKQNFSDEAWASFVKRIEYLKVDVTEAADFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAARGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT          505 a          420 c          480 g          424 t
ORIGIN      
        1 aaaggtatac cgtctgaaag tgggaagcgg gtgttattat agtacaaaat ttgtaatttt
       61 gttacaaaaa aaatcagaca tcgaaaagaa atttgattta aatcgcagaa tctttcgatt
      121 gctgtaacgg aataggggaa acctgcccga tttttggact aggtcaaata aattgtaaaa
      181 atgggaatag acaaaaagtg tagcggtgtg tagtattact actcattatg ccgcctgcct
      241 ttgtttccga tattgtgatg cagcgtgatg gggaaaacag gtttcaaacg gcatcgaaaa
      301 attctttcct gacgagataa tgcgatgagt acacaaacaa attttgattt ggtgttgttc
      361 ggtgcgaccg gcgatttggc aatgcgcaaa cttttgccct gcctgtatca ggcgcacgtg
      421 gccggtttgc ttcatcccga aggtcgtatt ttgggtgtga gccgcagcga attggatacc
      481 gccggttttt tggcaaaagc ggaaacgaat tctaaaatcc atatcaaaca aaatttttca
      541 gatgaagcgt gggcttcgtt cgtcaaacgc atcgaatacc tgaaggttga tgttaccgaa
      601 gcagccgatt ttgccgcttt gggcgatttg gtcaaagcgc gcaaagaaac cgacaacgtc
      661 gtcatctatc tgtccaccgc gcccaaattc ttcgcgcaag cctgtgaaaa ccttgctgcg
      721 atcggtttga atgccgacaa tgtgcgcgta gttttggaaa aaccgttggg tacggatttg
      781 gcttcttccc agcaaatcaa taccgatgtt gcgcgttatt tcaaagaagg gcaaatttac
      841 cgcatcgacc actaccttgg caaagaaagc ctgcaaaacc tgcttgcgct gcgttttgcc
      901 aatgtaatgt tcgagccgct gtggaacaat aaatatatcg aaagcgtgca gttgaccatc
      961 gccgaacagc tcggtgtgga agagcgcggt gagttttacg acattaccgg cgcgttgcgc
     1021 gatatggtgc aaaaccattt gatgcagatg ttgtgcatga ctgcgatgga agcccccgcc
     1081 agcttggatg ccgacgcggt gcgcgatgaa aaagtcaaag tcatcaagtc attgaagccg
     1141 ctgaccgtcg agtctgtcaa tgaaaacgtc gtgcgcggac aatataccgc cgccagaggc
     1201 atgaacggct atcttgaaga aatcaacgtt ccgcaagaca gctttaccga aacctacgtc
     1261 gccattaaag ccgaaatcga aaacgaacgc tggaagggcg ttcccttcta cctgcgtacc
     1321 ggcaaacgca tggcgggcaa agtggcggaa atcgttttga acttcaaaga tttgaacagc
     1381 catatttttg aaggcagccg caccgcgccc aaccggctcg ttatcgagtt gcaaccatat
     1441 gaatccgtgc gcctctatac gcagatgaaa accccggggg caggaaataa ggtcgaaacc
     1501 gtgccgctgg caaccgattt gggcaaagca ttggaaggcc gccgcgcgga agcttacgag
     1561 cgcctgctgc tggatgtgat taacggcaaa ctcgctttgt ttaaccgccg cgacgaactt
     1621 gaagccgcgt gggaatatgt gatgccgatt ttggaaaact ggacaaataa caccacgccg
     1681 ccgcacggct acggcgcaca ctcgtggggg cctgaagccg cgcgcgaact attggcgcgc
     1741 gacggacaca agtggcacga agagcagtaa tacaataatg cgttcagacg gcatggggtt
     1801 tgaaatgccg tctgaacata agtaaagca
//
LOCUS       DAWIVB010000231         1811 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWIVB010000231 DAWIVB010000000
VERSION     DAWIVB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.231"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(58..381)
                     /locus_tag="WIX60_001980"
     CDS             complement(58..381)
                     /locus_tag="WIX60_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ5306187.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(369..608)
                     /locus_tag="WIX60_001981"
     CDS             complement(369..608)
                     /locus_tag="WIX60_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ5306188.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(731..1528)
                     /gene="truA"
                     /locus_tag="WIX60_001982"
     CDS             complement(731..1528)
                     /gene="truA"
                     /locus_tag="WIX60_001982"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ5306189.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLVGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          405 a          430 c          519 g          457 t
ORIGIN      
        1 ccgtactggt ttaaagttaa tccactataa tgaacacaat ccattcagac tattcaatca
       61 ggcaaacatc tcctgcaata ctgcaaacag tttttcagcc gtactgttgt ctaaattgcc
      121 aagatgtttg accaatccgg ctttatccac agccctaatc tgttcgggca aaagcaaacc
      181 gtctttatcc tgaaagcgga cattgacgcg gaacggggca ggacggcttc cgctcgtcat
      241 gggaacgatc agcacagtct tgagatagtt gtgtatttca ggaggagaga ctacgacaca
      301 aggacgtgtc tttttgattt cgcttcctac ggtcgggtct aaggagacca gatagattcc
      361 gccgcgtact accatatcca ttctttatcc gcttcgtttt caatttcgga aaaaaaatgc
      421 tcctgctcgg tttcgacaag cattgcggca gcttctgccc atcccctgcg aacggtagga
      481 cagcttaaaa taatattgcc cttttcaact gtaacagcca agctgtctac tgcccctatt
      541 tgacccaata atgatttggg cagaatcacg ccttgcgagt ttcccatttt gcgtatgttg
      601 agaatcatat acgtaccata ttcacctgtt tatgtaataa caatgttagt accttgatga
      661 ggtagtgtca acatggaaaa agattgcctg acagtttgtc cgatttcaaa atctccgcga
      721 caagcatgtt ttaaagccat tcggggattt gggggcggat gatgccgtat gcctcgggat
      781 agtcgacgcc ggtcaggtaa agtccgtcgg gcatgaaggt cggcggggct ttgaggcggc
      841 tgcgttcttg aatcagtgcg gcgaagcctt cgacgctgag tctgccgctg ccgacataaa
      901 cgagcgcgcc catgatgttg cgtaccatgt ggtgcaaaaa ggcgttgccg tgcaaatcga
      961 ggcggacgag tcctgagctt tgggtaaggt cggcgcggta gatggttttg acgggggatt
     1021 ttgcttggca ttcggcggcg cggaagctgg agaagtcttg ttcgccgacc aataaggcgg
     1081 cagcctgccg catctgcccg atgtcgagtt tgaggtgtgt ccagcctgcc ctgtttttga
     1141 gcagggggga acggacgggg gcggattcga gcaggtagcg gtagtgccgt ccgtatgcgt
     1201 caaatcgtgc atgaaattcg ggggcgacct gtcgggcgtg caaaacggca atgccttcgg
     1261 gcaggtgggc atttacgccg cgcacccatg cctgttgggg acgggcggca gttgtgtcga
     1321 agtggacgac ttgggcggtg gcatgcacgc cggtgtcggt cctgccggca acggtggtgg
     1381 aaaccgcttc ccctgctatt tgggcgagcg cggtttccaa tgccgcctga acggtcggta
     1441 cgccgtcagc ctgtttctgc cagccgtaaa agcggctgcc gtcataggat agggttattg
     1501 cccagcgttg tttttgtgcg gtatccatcg gatttgggat tcggataaat gttcagactg
     1561 cattgtatcg cagattttgc agggaaacgg caaacgccca gggcgagcgg cgttgtttgg
     1621 ggagttgttg ggggggggat gcagttgcta cgaatcgcta tcctgtgaat ttaccctgtc
     1681 aggagtgccc gaatcgtcat tcccgcgcag gcgggaatct aggacgtaaa atctaaagaa
     1741 accattttat agtggattaa caaaaaccag tacagcgttg cctcgcttta gctcaaagag
     1801 aacgattctc t
//
LOCUS       DAWIVB010000232         1753 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWIVB010000232 DAWIVB010000000
VERSION     DAWIVB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.232"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            95..1036
                     /gene="mafA"
                     /locus_tag="WIX60_001983"
     CDS             95..1036
                     /gene="mafA"
                     /locus_tag="WIX60_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ5306190.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLITPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKKPDVGNEVIRRRKGG"
     gene            1040..>1753
                     /gene="mafB"
                     /locus_tag="WIX60_001984"
     CDS             1040..>1753
                     /gene="mafB"
                     /locus_tag="WIX60_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ5306191.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNP"
BASE COUNT          466 a          657 c          370 g          260 t
ORIGIN      
        1 tccgccgctc agacggcatt ttcgcccctt ttgtttacaa acccttaaaa tccctttaca
       61 ctcaaaatcc gttcaacatc aaacaaaccc cgctatgaaa accctgctcc tcctcatccc
      121 cctcgtcctc acagcctgcg gcacactgac cggcataccc gcccacggcg gcggcaaacg
      181 ctttgccgtc gaacaagaac tcgtcgccgc atcgtcccgc gccgccgtca aagaaatgga
      241 tttgtccgcc ctaaaaggac gcaaagccgc cctttacgtc tccgttatgg gcgaccaagg
      301 ttcgggcaac ataagcggcg gacgctactc tatcgacgca ctgatacgcg gcggctacca
      361 caacaacccc gaaagtgcca cccaatacag ctaccccgcc tacgacacta ccgccaccac
      421 caaatccgac gcgctctcca gcgtaaccac ttccacatcg cttttgaacg cccccgccgc
      481 cgccctgacg aaaaacagcg gacgcaaagg cgaacgctcc gccggactgt ccgtcaacgg
      541 cacgggcgac taccgcaacg aaaccctgct cgccaacccc cgcgacgttt ccttcctgac
      601 caacctcatc caaaccgtct tctacctgcg cggcatcgaa gtcgtaccgc ccgaatacgc
      661 cgacaccgac gtattcgtaa ccgtcgacgt attcggcacc gtccgcagcc gtaccgaact
      721 gcacctctac aacgccgaaa cccttaaagc ccaaaccaag ctcgaatatt tcgccgttga
      781 ccgcgacagc cggaaactgc tgattacccc taaaaccgcc gcctacgaat cccaatacca
      841 agaacaatac gccctttgga ccggccctta caaagtcagc aaaaccgtca aagcctcaga
      901 ccgcctgatg gtcgatttct ccgacattac cccctacggc gacacaaccg cccaaaaccg
      961 tcccgacttc aaacaaaaca acggtaaaaa acccgatgtc ggcaacgaag tcatccgccg
     1021 ccgcaaagga ggataaaccg tgaaaccgct gcgcagactg acaaacctcc ttgccgcctg
     1081 cgccgtagcg gcggccgcac tcatacagcc cgccctcgcg gcggacttgg cgcaagaccc
     1141 gttcattacc gataacgccc aacggcagca ctacgaaccc ggcggcaaat accacctctt
     1201 cggcgacccg cgcggcagcg tttccgaccg caccggcaaa atcaacgtca tccaagacta
     1261 tacccaccag atgggcaacc tgctcatcca acaggcaaac atcaacggca caatcggcta
     1321 ccacacccgc ttttccggac acggacacga agaacacgcc cccttcgaca accacgccgc
     1381 cgacagcgcg agcgaagaaa aaggcaacgt tgacgaaggc tttaccgtat accggctcaa
     1441 ctgggaagga cacgaacatc atcccgccga tgcctacgac ggcccgaagg gcggcaatta
     1501 ccccaaacct acgggcgcac gagacgaata cacctatcac gtcaacggca cagcccgcag
     1561 tatcaaactc aatccgaccg acacccgcag catccggcaa cgcatatccg acaactacaa
     1621 caacctcggc agcaatttct ccgaccgcgc cgatgaagcc aacagaaaaa tgttcgagca
     1681 caatgccaag ctcgaccgct ggggcaacag catggagttt atcaacggcg tcgccgccgg
     1741 cgcgctcaac ccc
//
LOCUS       DAWIVB010000233         1749 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWIVB010000233 DAWIVB010000000
VERSION     DAWIVB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.233"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            290..895
                     /locus_tag="WIX60_001985"
     CDS             290..895
                     /locus_tag="WIX60_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ5306192.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QLSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            958..1320
                     /gene="ssrA"
                     /locus_tag="WIX60_001986"
     tmRNA           958..1320
                     /gene="ssrA"
                     /locus_tag="WIX60_001986"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1638..1739)
                     /locus_tag="WIX60_001987"
                     /pseudo
     CDS             complement(1638..1739)
                     /locus_tag="WIX60_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611963.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          545 a          462 c          359 g          383 t
ORIGIN      
        1 tcagatttcc gtgtttcgcg tttccggcaa ttcaagattg gcaaaacacg tcagcctgct
       61 tcgcaaggca ggtttttaaa actttatcag gcgttcgggc atatcggttc gaccggtccg
      121 catttccggc aatcaacact gccgacggtt gccgcatttc tttttgatat acaatcacac
      181 tttcaaaaaa catacgcagg caacaaaatc atcctccccc gattggcaaa cattccggca
      241 aattaaaacc ccgcccgaaa cagccatcgg acaaataaca ggaaaaaata tgaataccga
      301 cttccaaaac atcctcaaac aactgggcaa acaagcccaa aaagaagcgg cagaaaaaca
      361 ggcagccgaa aaaaacaagc aaaaacaaga acaggatttc gatttttcac aagcagtcgg
      421 acaactctcc ccattaaaaa accggcagca atattatgcg ccgcccgaca aaacccccat
      481 caaagtccgt cccaaagaca accgcgccga cgaagaaaac tacttctaca tcggcagcac
      541 atacaacgac ccgcccgcca gcttcagcaa aaacggacag ggaaaaaacg acatccaacg
      601 cctgaaaaac ggatactatc ccgtcgttac cgatgtcgac ctgcacggct acacacagga
      661 agaagcccaa aaagtcctca acgaattcat cgcattcacc caaaaacgcg gcgtatgcgg
      721 cgaaatcatc cacggcagcg gattaggttc caaaggctac aaacccgttc tgaaaaatat
      781 gacccgaaac tggctgatgc aacaccccga cgtactcgcc tacgccgaac cccgggaagg
      841 caacgacggc tgcgtccgca tcctgctcaa acgcaaatta aggcagcaag actgacaaat
      901 ttctccgaca gacttggcag caccgcacaa tatccgtata atttgcaaac catttctggg
      961 ggcgaccttg gtttcgacga gggttgcgaa gcagatgcgg gcataccggg gtctcagatt
     1021 cccgtaaaac actgaattca aatagtcgca aacgacgaaa cttacgcttt agccgcttaa
     1081 ggctagccgt tgcagcagtc ggtcaatggg ctgtgtggcg aaagccaccg caacgtcatc
     1141 ttacattgac tggtttccta ccgggttact tggcaggaaa taagacttaa ggtaactggt
     1201 ttccaaaagg cctgttggtc ggcatgatgg aaataagatt ttcaaataga cacaactaag
     1261 tatgtagaac gctttgtaga ggactttcgg acgggggttc gattcccccc gcctccacca
     1321 taccctattt tcagcagcat cctgcaacac aaaaaagcct tgaacttcaa cagtttcaag
     1381 gcttttttgt gttgcaggat gctggcgtat gcttgagttt tcgccattta tggggtaact
     1441 tttggggtaa cttttcacga attaccccaa aaaacgcctt aaaaatgcaa aagtcatcac
     1501 gcattttttt aaaaaggtta cccaaaactt aaatgtaatc ctttatttat aagcttgcag
     1561 ctttccaaac aagttacccc caaaacggat aaaaaaatgc cgaagccgaa ctgaccccaa
     1621 aaagtcggac agtttaatca agcggctttc agggactgag ttctgtactg aacagggctc
     1681 agtcctttta atttcaactt gattctatcg ttgttgtagt aacggatata ttcgtgcagt
     1741 gccgcttac
//
LOCUS       DAWIVB010000234         1740 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWIVB010000234 DAWIVB010000000
VERSION     DAWIVB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.234"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            30..917
                     /gene="prmA"
                     /locus_tag="WIX60_001988"
     CDS             30..917
                     /gene="prmA"
                     /locus_tag="WIX60_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ5306193.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTATQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEVPEGSAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGFAVGVDIDEQAVRAGKDNAAQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            935..1498
                     /gene="orn"
                     /locus_tag="WIX60_001989"
     CDS             935..1498
                     /gene="orn"
                     /locus_tag="WIX60_001989"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ5306194.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDDVLNKMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
BASE COUNT          460 a          533 c          444 g          303 t
ORIGIN      
        1 aatccactat atttccaaga aagcccgtta tgccctacca acaaatcacc gtcaacgtca
       61 acgatgccgt cgccgaacgc ctcgccgacg cgctgatgga acacggcgca ctctccgccg
      121 ccatcgaaga tgcctacgcc ggcacgcaaa acgaacaggc gattttcggc gaacccggta
      181 tgcccgccga acaaatctgg cagcagagca aagtcatcgc cctgttcggc gaacacgacg
      241 aagccgccgc catcatccaa accgccacac aagaatgcgg gttaaaagac ttggcataca
      301 ccggcgaaac catcgaagac caagactggg tgcgtctcac gcaatcgcaa ttcgacccca
      361 tccggatttc cgaccgcctg tggattaccc cctcttggca cgaagtcccc gaaggcagtg
      421 ccgtcaacct ccgcctcgac cccggactcg ccttcggcac cggcagccac ccgaccacgc
      481 gcctctgcct caaatggttg gatacgcaac tcaaaaacgg cgaaagcgtc ctcgactacg
      541 gctgcggttc gggcatcctg accatcgccg ccctcaaact cggtgcaggt ttcgccgtcg
      601 gcgtggatat tgacgaacag gccgtccgcg ccggcaagga caacgccgcg caaaacaacg
      661 tcgatgcaca attcttcctg cccgacggtc tgcctcaagg gcaattcgac gtagttgtcg
      721 ccaacatcct cgccaaccct ttgcgtatgc ttggcgaaat gctcgccgcc cgcaccaaac
      781 agggcggacg catcgtgttg tccggtttgt tggacgaaca ggccgaagaa ctcggcggca
      841 tttacagcca atggttcgac ctcgacccgg cggaaaccga ggaaggatgg gcgcgattga
      901 gcggcgtaaa acgctgaaac ggaaaggaaa caccgtgcag gataaaaaca acctctgctg
      961 gctcgatatg gaaatgacgg ggctgaatcc cgaaaccgac cgcattatcg aagtcgcgat
     1021 gattattacc gactcggatt tgaatgtgtt ggcgcaatcc gaagtttacg ccgtccacca
     1081 aagcgacgac gtgctgaaca aaatggacga atggaacacc gccacacacg gcaggacggg
     1141 gctgacacag cgcgtacgcg aatcgtcgca taccgaagcc gaagtcgaac agaaactgct
     1201 ggactttatg tcggaatggg tacccggacg cgccacgccg atgtgcggca actccatcca
     1261 ccaagaccgg cgttttatgg tcaaatatat gccgaaactg gaaaactact tccactaccg
     1321 caacctcgac gtttccacgc tgaaagaact cgccaaacgc tggaatccgc ccgttgccaa
     1381 aagcgtcgtc aaacgcggtt cgcacaaggc attggacgac attttggaga gcatcgaaga
     1441 aatgcgccac taccgcgaac actttctgat ttccgccccg agagccgaag cgcaataaga
     1501 aacaaacaat gccgtctgaa acgcagtttg catttcagac ggcattttta cagcagattg
     1561 aaatcaaaaa tatacacgcc cgtcattccc gcacaggcgg gaatccggaa ggtcgggcct
     1621 gccgttattt tcaatcatta cagaaactga aaggtctgga ttcccgcctg cgcgggaatg
     1681 acgggcgtgt gcattcttat agtggattaa caaaaatcag gacaaggcga cgaagccgca
//
LOCUS       DAWIVB010000235         1728 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWIVB010000235 DAWIVB010000000
VERSION     DAWIVB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.235"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(521..1444)
                     /locus_tag="WIX60_001990"
     CDS             complement(521..1444)
                     /locus_tag="WIX60_001990"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ5306195.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          456 a          431 c          423 g          418 t
ORIGIN      
        1 ctttcgtggg aatgacggga tttgagattg cggcatttat cggaaaaaca gcaaccgctc
       61 cgccgtcatt cccgcgcagg cgggaatcca gaccttagaa taacagcaat attcaaagat
      121 tatctgaaag tccgggattc tagattccca ctttcgtggg aatgacggca tcagtctgcc
      181 gtttacagca cggtttcttt agattttacg ttctagattc ccgcctgcgc gggaatgacg
      241 aatccatcca tacgaaaacc tgcaccacgt cattcccacg aacctacatc ccgtcattcc
      301 cacaaaaaca gaaacctcaa atcccgtcat tcccgcgcag gcgggaatcc agacttgtcg
      361 gtgcggacgc ttatcggata aaacggtttc ttgagattcc gcgtcctgga ttcccacttt
      421 cgtgggaatg acgaatttta ggttgctgtt ttggtttttt gtccttgtgg gaatgatgaa
      481 aatttaagtt ttaggaattt accggaaaaa acagaaagcg ttatccacaa tttctgatgt
      541 tcagctcgtg aaatgcgtcg ggcaaatcat cgctgtcggc aaattccacc cggtcgtaag
      601 ccgtttcgtc tgccaaaacc gcgcgcaaga gtgcgttgtt gatggcgtgt cccgatttgt
      661 agccttcaaa tgcgccgaca atcggatgtc cgacgatata caaatcaccg atggcatcaa
      721 ggattttgtg gcgcacaaac tcatcgggat agcgcaagcc ttcaggattc aggacatccg
      781 tgtcgtcaat cacgatggcg ttgttcaaat tgccgcccaa acccagattg tgggcgcgca
      841 tcatttccac ttcgtgcata aagccgaaag tgcgcgcgcg cgcgatttcg tcgatgtagg
      901 atttgccggc gaaatcaatt tcaaaagtgg gcgagctgcg gttgaaaacc ggatggtcga
      961 attcgatggt cagcgttacc ttaaagccgt catacggcgt aaagcgcacc catttgcccg
     1021 cttctttgat ttcgacaggc ttgaggattt tcaaaaaacg cttttgcgcc ttttgatcga
     1081 ccacgcccgc atcttgcaaa aggtaaataa acggcaggct ggagccgtcc ataatcggga
     1141 tttcgggtgc gttcagctca atcagcgcgt tgtcgatacc gtaggcggac agcgcggaca
     1201 taatgtgttc gatcgtgccg acgcgcacgc ctttgtcggt aacgatggtg gaggaaaggc
     1261 gggtatcgtt gatcaaataa ggggtcagct tgatttgttc gcccatctcg ccgtccaaat
     1321 cggtacggcg gaaggaaatc ccgctgtttt caggcgcggg gtgcagggtc agcgcgacgc
     1381 gttcgccgga atgcagcccg actccggtaa cgctgatgga tttcgccaaa gttctttgca
     1441 gcataaaccg cttccttatc aatggggtaa gttttggaat aatacgataa aaccggaaaa
     1501 acaagctatg tttttccata gtatttgcca atgtatccgt tttcaatacg taagccgcat
     1561 aaaaatgtat agtggattaa caaaaatcaa gacaaggcga cgaagccgcc cccctcctga
     1621 aaaacgcaaa aaatgccggc cgaaaacctt tcggacggca ttttcgcgta aaccgtcatt
     1681 cccacaagga caaaaaacca aaacataaaa ccaaaaacag caacctaa
//
LOCUS       DAWIVB010000236         1723 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWIVB010000236 DAWIVB010000000
VERSION     DAWIVB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.236"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WIX60_001991"
     CDS             complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WIX60_001991"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ5306196.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVETVCEAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKYGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAE"
     gene            complement(1394..1567)
                     /locus_tag="WIX60_001992"
     CDS             complement(1394..1567)
                     /locus_tag="WIX60_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ5306197.1"
                     /translation="MKSERRHWEWCVDYDGEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
BASE COUNT          375 a          498 c          468 g          382 t
ORIGIN      
        1 caatctgcca aacagatcga acagagaaac cctgtccgtc aaaacatcat tcagccatcg
       61 ccttgaacac ttcaaccgca accgcaaccg tttcgtcaat cagctcgggc gtatgcgcgg
      121 cggaaacgaa acctgcttca taagcggacg ggccgaaggc gacattgcgg tcgagcatcc
      181 cgtggaaaaa ctgtttgaag ccttcaatat tggaacgcgc catatcggca tagtttcgcg
      241 gcgcgtgtgc ggcgaaatac agaccgaaca taccgcccac gctgtcggcg gtgaactcga
      301 tgcccgccgc atccgctgcc gtccgaaaac cttgaaccaa ctgttcggta cgcgccgtca
      361 ggttttcata gaagccttcg cgctggatga tttccagcgt tttcaagcct gcggcgacag
      421 caatcgggtt gcccgacaag gtgcctgcct gatacacgcc gcccagcggg gaaatacatt
      481 ccataatgtc tttgcgcccg ccaaacgcgg caagcggcat accgccgccg atgactttgc
      541 ccatcgtggt caggtcgggc gtgatgccgt gcaaagattg cgcgccgccg agcgcgacgc
      601 ggaagccggt catcacttcg tcgtaaatca acaccgcgcc gtatttttcg gtcaatccgc
      661 gcaaggcttt gacaaaggct tcggtcgggc ggacgaggtt catattgccg acgaagggtt
      721 cgacaatcac gcaggcgatt tcattgccgc tttgagcaaa ggcttcttcg agttgggcga
      781 tattgttgta ctcgagtacc aaagtgtgtt tggtaaagtc ggcaggcaca ccggcggaag
      841 acgggttgcc aaacgtcagc agaccgctgc cggctttcac cagcaggctg tcggaatgcc
      901 cgtggtagca gccttcaaac ttgatgattt tgtcacgccc ggtaaaaccg cgtgccagac
      961 ggatggcggt catggtcgct tcggtaccgg agctgacgag gcgcagccgt tcgacggacg
     1021 gcatgatttt ggcgatttct tcggcaatga cgatttcgcc ttcggtaggc gcgccgaacg
     1081 acaaaccgcc caatgcggct tcgcatacgg tttcgacgac ttcggggtgc gcgtgtccga
     1141 caatcgcagg tccccacgag ccgacgtaat cggtatagcg cgtgccgttt tcgtcccaaa
     1201 catacgcgcc ttcggctttt ttgataaagc gcggtacgcc gccgacgctg ccgaatgcgc
     1261 ggacggggga attcacgccg ccggggatga tggctttggc gcggtcgaat aaaatttcgt
     1321 tacggttcat atatatcctc aaatgccgtc tgaacggcag gtttcgggct tggaagcaga
     1381 aagccccatt ttatcatttt tcaggttgcg acaaggattt gcccgcttct ttgcggatca
     1441 cgccaaccgc atcccggatg acggaacgct cgtctttttc cactttatgt gtaaagcggt
     1501 agtctcggac gactccctcc ccgtcgtaat ccacacacca ctcccaatgt cggcgttctg
     1561 atttcatata aatgaaattg gtcggcaaaa aattataaat cggcaggctg acttcatgat
     1621 aggcataaca accgaaaggg ttgcgcttcc cgaaacgtgc ctctacacct ccgcccgggt
     1681 cgttttgcct ttaacaaccg tttgtgcgat tccctcttcc gtc
//
LOCUS       DAWIVB010000237         1718 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWIVB010000237 DAWIVB010000000
VERSION     DAWIVB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.237"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            342..1646
                     /locus_tag="WIX60_001993"
     CDS             342..1646
                     /locus_tag="WIX60_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ5306198.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGVVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          351 a          431 c          509 g          427 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattg gcggcacatc ggcaaaatcc
      241 ccgcgccgcc ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt
      301 tccgaaagac gggctcaacc attaaggaaa ctttagtcaa aatgaaaaaa cacatatggg
      361 cggcatcttt gctgccggca tccctatcgg cagaaccttt aaactggtgg aagccttatt
      421 ccgccgtcaa ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga
      481 tgacgcttcc cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct
      541 cgacgatgat gcacagcttt tccatcgcga cattggtggg catcctttgg gtcgccgtcg
      601 gctattcttt agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt
      661 taagcgggat gcagatagac gctaccgcac agatgctgac cgtgtcgccc aatgcgccga
      721 ctgttcccga accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca
      781 ttattaccgg cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca
      841 tatggttttt attggtttat gtgccgggcg cgcattgggt gtggggcggc ggctttatga
      901 gcaagggcgg cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg
      961 cgggactcgt cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc
     1021 ctccgcacaa tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg
     1081 gctttaacgc cggatcggcg cttgcggcag acgcggcggc gggtatggcg atggcggtaa
     1141 cgcaggtgtc ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac
     1201 acaaaccttc cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc
     1261 ctgccgccgg ctttaccggc ccgtcgggcg cggccgccat cggtatattg actgccgccg
     1321 cgtgctttgt gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg
     1381 ctttcggcat acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg
     1441 acaaccgcat tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag
     1501 acggggtcgt tatggcggca tacagcgggc taatgagttg ggcgattttg aaggtcgtgg
     1561 ggaaaatctg cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga
     1621 atatccacgg cgaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg
     1681 tttcagacgg catttttcac gtttgccgcc gatggata
//
LOCUS       DAWIVB010000238         1688 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWIVB010000238 DAWIVB010000000
VERSION     DAWIVB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.238"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     regulatory      75..119
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            124..597
                     /gene="queF"
                     /locus_tag="WIX60_001994"
     CDS             124..597
                     /gene="queF"
                     /locus_tag="WIX60_001994"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ5306199.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFETLARKRLFEHDAQ"
     gene            672..1358
                     /locus_tag="WIX60_001995"
     CDS             672..1358
                     /locus_tag="WIX60_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ5306200.1"
                     /translation="MYAFTAAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYAIGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT          402 a          511 c          357 g          418 t
ORIGIN      
        1 cacggaaaac gccgccgtcc aaaccatgcc gtctgaagaa aactacacag ataccgccgc
       61 ttatattaca atcgccgccc gtggttcgaa aacctcccac attaaaaaac taaggaaacc
      121 ctcatgtccc gcaacaacga agagctgcaa ggcatctctc ttttgggcaa tcaaaaaacc
      181 caatatccga ccggctacgc gcctgaaatt ctcgaagcat tcgacaacaa acatcccgac
      241 aacgactatt tcgtcaaatt cgtctgtccc gagttcacca gcctctgccc catgaccggg
      301 cagcccgact tcgccaccat ctacatccgc tacattccgc acatcaaaat ggtggaaagc
      361 aaatccctga aactctacct cttcagcttc cgcaaccacg gcgattttca tgaagactgc
      421 gtcaacatca tcatgaaaga cctcattgcc ctgatggatc cgaaatacat cgaagtattc
      481 ggcgagttca caccgcgcgg cggcatcgcc attcatccgt ttgccaacta cggaaaagca
      541 ggcaccgagt tcgaaacatt ggcacgcaaa cgcttgtttg agcacgacgc acaataaacc
      601 atccttccca tatttcagac ggcattcctt cgatacgata tgccgtctga acgcctttcc
      661 gaaagatttt tatgtacgca tttaccgccg cacagcaaca gaaggcactc ttccggctgg
      721 tgctttttca tatcctcatc atcgccgcca gcaactatct ggtgcagttc cctttccaaa
      781 ttttcggcat ccacaccact tggggcgcat tttcctttcc cttcatcttc cttgccaccg
      841 acctgaccgt ccgcattttc ggttctcact tggcacggcg gattatcttt tgggtgatgt
      901 tccccgccct tttgctttcc tacgtctttt ccgttttgtt ccacaacggc agttggacag
      961 gcttgggcgc gctgtccgaa ttcaacacct ttgtcggacg catcgcctta gccagctttg
     1021 ccgcctacgc gatcggacaa atccttgata tttttgtatt caacaaatta cgccgtctga
     1081 aagcgtggtg gattgcaccg accgcatcaa ccgtcatcgg caacgccttg gatacgctgg
     1141 tatttttcgc cgttgccttc tacgcaagca gcgatggatt tatggcggca aactggcagg
     1201 gcatcgcttt tgtcgattac ctgttcaaac ttaccgtctg caccctcttc ttcctgcccg
     1261 cctacggcgt gatactgaat ctgctgacga aaaaactgac aaccctgcaa accaaacagg
     1321 cgcaagaccg ccccgcgccc tcgctgcaaa atccgtaaaa cagccctgcc gggaatcggg
     1381 acggttgggt tttcttttat ttgcaaacac tgtcggaacg gataaatccg gattcccgcc
     1441 cacttcgtca ttcccgcgaa agcgggaatc cggtacgttg ggtttcagtc atttccgata
     1501 aactcctgcc gcgttgtatt tctagattcc cgctttcgtg ggaatgacag cggatgggtt
     1561 tccgtgccgt ggtgtcagga ttcccgcccg tacgggaatg acggcagtgc ttccttggct
     1621 tagttttgtt ttaatcaact gcatagtgga ttaacaaaaa tcaggacaag acgacgaagc
     1681 cgcagaca
//
LOCUS       DAWIVB010000239         1687 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWIVB010000239 DAWIVB010000000
VERSION     DAWIVB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.239"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(195..1370)
                     /locus_tag="WIX60_001996"
     CDS             complement(195..1370)
                     /locus_tag="WIX60_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ5306201.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQ
                     AANGGASGQVKVTKVTKAKSRIRTKISDFGSFIGFKGSEDLGDGLKAVWQLEQDVSVA
                     GGGATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRH
                     DDMPVSVRYDSPEFSGFSGSVQFVPIQNSKSAYTPAYYTNTNNNLTLVPAVVGKPGSD
                     VYYAGLNYKNGGFAGNYAFKYARHANVGRNAFELFLIGSGSDQAKGTDPLKNHQVHRL
                     TGGYEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIE
                     RGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          438 a          426 c          388 g          435 t
ORIGIN      
        1 tatatgtgca aaccaagcca aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaag gcgagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgataaagtc gaaaccatgg gcatagctga tgcgtggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt gtcgccattt
      481 tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcctc atagccgccc
      541 gtcagacggt gtacctgatg gtttttcaag ggatcggtac ctttggcttg atcactcccg
      601 ctgccgatca agaacaactc aaaagcatta cgtccgacat tggcgtgtct cgcatattta
      661 aaggcatagt tcccggcaaa accgccattt ttgtaattca gaccggcata atacacatcc
      721 gatccgggct tgccgacaac agccggaacg agagtaagat tattgtttgt attagtataa
      781 taagccggcg tataggcgga cttgctgttt tggatcggaa cgaattgaac gctgccgctg
      841 aaaccggaaa attcggggga atcgtagcgt acggaaaccg gcatgtcgtc gtggcgtttg
      901 aaaataccca attgcgaagc cacatcatta ttgctgtccc aaggatcaat ggcttggctg
      961 gcatcgtcaa actgattcgc aacgcgaccg gcgcgcagcg taccgaattc gcctgccaag
     1021 ccgataaagg attccctgtt gccccactgg gtcgcgccgc cgccggcaac ggatacgtct
     1081 tgctcaagct gccaaacagc cttcagcccg tcgcccaaat cctcactccc cttaaagccg
     1141 ataaacgagc cgaaatcact gattttcgtc ctgatgcggc ttttggcctt agtaacttta
     1201 gtaactttta cctgaccgct cgctccaccg ttagcggctt gtgcttcagt caattgcagc
     1261 tggtagttcc tgccttccac gccggctttg atttcgccgt ataggctgac atcggcaacg
     1321 gccgcaagcg gcagtgcgga caatacgagg gcggtaagtt tttttcgcat atcggcttcc
     1381 ttttgtaaat ttgataaaaa cctaaaaaca tcgggcaaac acccgatacg tcttcaatta
     1441 tacccccccc cccgcaaaaa accatttttc agaacaaata tctgataaat gccgcaacct
     1501 ttattttaaa aatgattata ttttgatata aaacaatagc ttattttttc aaaaacgttg
     1561 tgtttctaca acacaattca agcgcagacc tcgtgcgagc cgatgcgctg ctgcccggat
     1621 gcagtctcgg ctttttaaaa cgccataaaa aaacacacgc ggcactttat agtggattaa
     1681 caaaaac
//
LOCUS       DAWIVB010000240         1675 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWIVB010000240 DAWIVB010000000
VERSION     DAWIVB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.240"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            1..449
                     /locus_tag="WIX60_001997"
                     /pseudo
     CDS             1..449
                     /locus_tag="WIX60_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581202.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
     gene            621..1502
                     /locus_tag="WIX60_001998"
     CDS             621..1502
                     /locus_tag="WIX60_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306202.1"
                     /translation="MRTDAFKYGTWNSLNDIRSSYDRAATKIKGAGNMGLGATTFVGS
                     GAIGGGLCSTGIGCAAGGLIATAGMTGGYTQASEGSRQLFGTYQSDFGKKVVLSLGTP
                     IEYESPLVSDAKNLAVWGLETLITRKLGNLATGVKTSLTPKTADVQRNILSQSEVGIK
                     WGKGIEGQGMPWEDYVGKGLSANARLPKNFKTFDYFDRGTGTAISAKTLDTQTTARLS
                     KPEQLYSTMKGYIDKTANFKSYELSEVPLRADMIKQREIHLAIPAQTNKEQRLQLQRV
                     VEYGKSQNITVKITEIE"
     gene            1503..>1675
                     /locus_tag="WIX60_001999"
     CDS             1503..>1675
                     /locus_tag="WIX60_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ5306203.1"
                     /translation="MTFNQEQDYWAGYKANERALIIQTWSGFGRYAPDHLYPPHILPL
                     DTDNETLGTTVLQA"
BASE COUNT          518 a          384 c          438 g          335 t
ORIGIN      
        1 accgaaactg cggatcaaca ctcaggccgt ctgaaaaaca gcttcgacaa agacgcggtc
       61 gccaaagaga tcaacctgca aagggaagta acgaaggagt tcggcagaaa cgccacccaa
      121 gccgtagcgg ccgttgccga caaactcggc aatacccaaa gttacgaacg gtatcaggaa
      181 gcccgaaccc tgctggaggc cgaactgcaa aacacggaca gcgaagccga aaaagccgcc
      241 ttccgcgcat ccctcggcca agtaaacgcc tatcttgccg aaaaccaaag ccgctacgac
      301 acctggaaag aaggcggcat aggcaggagc atactgcacg gggcggggcg gcaatcggct
      361 atgcggcggg cgggaatgtc ggtacggcgg cagtaggggc gaatgtcgat tggaacaata
      421 ggcagctgca tccgaaagaa acacaaatcc ttaacaaact gtcaaaaggc aaatcggctg
      481 aagaacagta ccgcctaaaa gccgctgcat gtgcattaac ccggtgcgcg gaaggcgtac
      541 ctgacttcga ccctctttat aaaggactaa aaaacctcca agatgccggt aaacagtttg
      601 tagcggaaca aaacgtattg atgcggacgg atgcatttaa atatggaaca tggaacagcc
      661 tgaatgatat acgcagcagt tacgaccgtg ctgccaccaa aattaagggt gcgggcaata
      721 tgggattggg tgcaacgact tttgtcggtt cgggtgctat aggcggaggt ctgtgcagta
      781 ccgggattgg ctgtgcggcc ggtggactta ttgcaacggc aggtatgacc ggtggttata
      841 cacaggcctc agaaggaagc cggcaattgt ttggcactta ccagtccgat tttggtaaaa
      901 aagttgtcct atctttgggt acaccaatag aatacgaatc gccgttagta tctgatgcga
      961 aaaatctagc cgtatgggga ttggaaacgc tgattacgcg caaattggga aacttggcaa
     1021 cgggtgtgaa aacttccttg actccgaaaa ctgctgacgt acagcgaaat atcctgtccc
     1081 aatccgaagt cggtatcaag tggggcaagg ggattgaagg acagggaatg ccttgggagg
     1141 attatgtcgg taagggcttg tctgccaatg caaggttacc taaaaatttt aaaacatttg
     1201 attattttga tcgtggtaca ggcacggcaa tcagtgccaa aactctggat acgcaaacta
     1261 cggcacgcct gtccaaaccc gaacagcttt acagtaccat gaaagggtac atcgataaga
     1321 cggcaaattt caaaagttat gaattatcag aagtaccgtt aagggcagac atgatcaaac
     1381 agcgcgaaat ccatctggcc atacccgcac aaactaataa ggagcaaaga ttgcagttgc
     1441 aacgtgtggt agagtatggc aaaagtcaaa acattacagt caaaattacg gagatcgaat
     1501 aaatgacttt caatcaagaa caagattatt gggctggcta taaggcaaat gaaagagcct
     1561 tgattattca aacatggtca ggatttgggc gatatgctcc agaccaccta tatccccccc
     1621 atatcctgcc attggatacc gacaatgaaa ctttaggcac aacggtcttg caagc
//
LOCUS       DAWIVB010000241         1669 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWIVB010000241 DAWIVB010000000
VERSION     DAWIVB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.241"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            1..222
                     /locus_tag="WIX60_002000"
                     /pseudo
     CDS             1..222
                     /locus_tag="WIX60_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            346..1041
                     /locus_tag="WIX60_002001"
     CDS             346..1041
                     /locus_tag="WIX60_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ5306204.1"
                     /translation="MGDGCRNGINMSIKYIAMPAAWKYMPTDVSRVAKEVFGYLYNSQ
                     GASTRFDKYFNTIDNRADFFAASNLYEQNLGSKARWFGGADFVSRAAITGLGADGEAS
                     YITFAAGKVVGNPPIYEWRAASGNALIVNGFYNFRDLFNKKTNPREWDIQQLKSEQKL
                     LQPIHQKYLSNEKDYLSLIKGVTSNKIFSIIPNPLDERKKIEDGINMLDYKSRIKYGC
                     KLMGYSEKQGCKP"
     gene            1029..1472
                     /locus_tag="WIX60_002002"
     CDS             1029..1472
                     /locus_tag="WIX60_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306205.1"
                     /translation="MQAMNKNYLFYLIQNIKSIFLIGGVFFGLLIFSGLWGNDFKLSF
                     LGVIDFIFGMVGGGFIVMLWFLIPVILSTLFRWVFQNQLAVSVILTFIVFYTYGYFSL
                     FNSSERVGSFWYLVIPTIMFIGLSEWFIYKSSVHSLGENVTPRSN"
     gene            complement(1540..>1669)
                     /locus_tag="WIX60_002003"
     CDS             complement(1540..>1669)
                     /locus_tag="WIX60_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEZ5306206.1"
                     /translation="FRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPSIP"
BASE COUNT          514 a          244 c          382 g          529 t
ORIGIN      
        1 gcgataggag cggcggtcgg tgaaatcctc ggcgaagccc tgctggacgg cagagacccg
       61 ggcagcctga atgtgaaaga ccgggcaaaa atcattgcta aggcgaagct ggcagcaggg
      121 acggttgcgg cgttgagtaa gggggatgtg aatgctgcgg cgaatgcggc tgctgtggcg
      181 gtagagagta atgcgcttag caaggaaaga atggataaat tgacaaaatg cctttccggt
      241 aaaacttgtt ctactacgat ggaaaaagta aatgccatca aaaaggatga acaatttagc
      301 aaagtaattg atacggaaat tcaaaaagtc tgttctagga acccattggg cgatggttgc
      361 agaaacggca ttaatatgtc tattaaatat attgccatgc ctgctgcgtg gaagtatatg
      421 cctacggatg tatcacgggt tgccaaagaa gtttttggct atttatataa ctcacaaggg
      481 gcatctacaa gatttgacaa gtatttcaac accattgaca atcgtgcaga tttctttgct
      541 gccagcaatc tttatgagca aaatttgggt tcaaaagcac gatggtttgg tggagctgat
      601 tttgtatcgc gtgctgctat aactgggtta ggggcagacg gagaagcttc ttatataact
      661 tttgcggcag gtaaagttgt tggtaatcct ccaatttatg aatggagggc tgcgtcaggc
      721 aatgcactga tagtaaatgg attttataat tttagagact tgttcaataa aaaaactaat
      781 cctagggagt gggatattca acagttgaaa agcgagcaaa agttattaca gcctattcac
      841 caaaaatatt tgagtaatga gaaggattat ttgtcattga ttaaaggggt aacatcaaat
      901 aagatatttt caataattcc aaatccttta gatgagagga aaaaaataga ggatggaatt
      961 aatatgttag attacaaatc tagaattaaa tatggttgta agcttatggg atattctgag
     1021 aaacagggat gcaagccatg aataaaaatt atttatttta tcttattcaa aatattaaat
     1081 ctattttctt aataggtgga gtattttttg gccttttaat tttttcaggt ttatggggta
     1141 atgattttaa actatctttt ttaggggtaa tagattttat atttgggatg gtagggggtg
     1201 ggtttatagt tatgttatgg tttctaattc cagtgatatt atcaacttta ttcagatggg
     1261 tatttcaaaa tcaacttgct gtatctgtca tattaacctt cattgttttt tatacatatg
     1321 ggtacttttc tttatttaat tcatctgaaa gagtcggtag tttttggtat ttggtaatcc
     1381 caaccataat gtttattgga ctgtctgaat ggtttatcta taaatctagc gtgcatagtt
     1441 taggtgaaaa tgtaactcca cgttccaatt gaagaaaaag attgtctgaa aactaaattt
     1501 aatttcagat gaccttagat tcggatttca agtacaacac tagggtatcg atggttggaa
     1561 caaattcaag aataaaacac ttggcgtttc gtagccaagt gtttttctcg gtcggtggtt
     1621 caactcatct tgaaccctac gtatctcccg atcgctgatg tttcggaaa
//
LOCUS       DAWIVB010000242         1646 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWIVB010000242 DAWIVB010000000
VERSION     DAWIVB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.242"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(18..680)
                     /locus_tag="WIX60_002004"
     CDS             complement(18..680)
                     /locus_tag="WIX60_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ5306207.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLDEYDSRYAQDTTLFDGVNELIAELGKRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMTTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(718..1545)
                     /locus_tag="WIX60_002005"
     CDS             complement(718..1545)
                     /locus_tag="WIX60_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ5306208.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAINLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
BASE COUNT          316 a          436 c          506 g          388 t
ORIGIN      
        1 tgtggggcgg atatttttca gggctgtatt ttgtccagac attcgagcag atcgagtggc
       61 gtgcggatgt ggaaatccgc ctgccatgag ccggtatcgt cttcgggagc gatgtagccc
      121 cattcggcga ggacggtcgt cataccggcg ttgcgccccg cctgtatatc gcgttccgcg
      181 tcgccgacgt agagtgtgtg ttgcgggtcg gcgtggattt gtccgcacgc atacagcatg
      241 ggtttgacgc tgggcttggg ctcgccgcag gtgtcgccgc tgacgacgac ggcgggtggg
      301 atgatgaagc cgagtttggg gacgagtttg tcggtgaagc gcatgggttt gttggtgatg
      361 atgccccatt tgatgccgcg ttttccgagt tcggcgatga gttcgtttac gccgtcgaag
      421 agggtggtgt cttgggcgta gcggctgtcg tattcgtcaa ggtattcggt gcgccatcgg
      481 gcatagtcgg gatggtcggg ggtgatgcct gcgccgagct tgatcagtcc tgccgcgccg
      541 tggctggctt gggtgcggat ttcgtccatg ctttttgcag gtagtccgtg gcgggcgagc
      601 agggtgttga gtgcgccgcc gaggtctagg gcggtgtcgg cgagcgtgcc atcgaggtcg
      661 aacaatacgg cttgtatcat gtgtgttcct tttttataaa gtgcgggacg aagggtttca
      721 gacggcatgt ttattttgtt tcaaaccctg ctcgaaatct tccaacatat ccaattcaaa
      781 gcggctgaag cctgcttttt cgcgcgcttc gatgttcaca tagccccgga agataaacat
      841 atcgtaacgg gcaatcaggc tgcggaacag ggcgacaggc tccaaaccgc gttcgcggca
      901 aaggtgttga taccaccggt tgccgatggc gacgtgtccc acttcgtcgc ggtaaatgat
      961 gtccaacacg ccgcaggttt ccgaatcacc gcgctgcgcc accttcgcgc gtatgccggg
     1021 cgtaacgtcc agcccgcgcg cttccaaaac gcgcggcact aaagccatac gcaacaaagg
     1081 atcgtaggcg gttttgtatg ccatatccca taaatgattg tgtgcttcaa aatcgccgta
     1141 atcgaagccg aaagcgcgca gcctttcgcg catcaggcgg aaatggtaca cctcttcctt
     1201 cgccactttc acccagtcgc ggacaaactg aaacggcagc gtgcggaaac ggtatgccgc
     1261 gtccaaagcc agattgatgg cgttgaattc gatatgcgca atcgcgtgca gcatcgccgc
     1321 atagccttcg gttgtgttca ttttgcgtgg cgtcagctgc gacggcgcga ccaaaacagg
     1381 cttgtccggt cgtcccgcgc gggggaagtc cgccggcggt gcgtttgttt ccgccccgtc
     1441 cgcattttga acggcggcaa acgcctcatc cgtcagccgt cctttttcat ctgggtcgcc
     1501 cgaaagcagg gcgcgttcca gcaaagcata aatatcgggt ttcatctcaa gtccgccgtg
     1561 ttcggaaaac aaatattata gcgtttaaaa aaaacaagat gaggcatata atctccgcga
     1621 ttcggcattc cgcgcccaaa ccgtca
//
LOCUS       DAWIVB010000243         1625 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWIVB010000243 DAWIVB010000000
VERSION     DAWIVB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.243"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            63..1505
                     /gene="aldA"
                     /locus_tag="WIX60_002006"
     CDS             63..1505
                     /gene="aldA"
                     /locus_tag="WIX60_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ5306209.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
BASE COUNT          404 a          463 c          432 g          326 t
ORIGIN      
        1 tacggcaatt tatttgggaa taaactaaaa ctacatctaa ctacaaaact ggagaacccg
       61 aaatgaaaca attggccatg tacatcaacg gacgctttga aaacgatttc aacggcgaat
      121 ggcgcgacgt attgaacccg tccaccgaag aggccatcgc ccgcgaaccc aaaggcggca
      181 aggcggacgt tgaccgcgcc gtcgcggcgg cgcgtgcggc gcaaccggct tgggagcgtc
      241 tgcctgcggt cgaacgcggc gcgtatttgc gtaaaatcgc ccaaggcata cgcgaacgtg
      301 ccgacgagct gaccgacacc atcgttgccg aaggcggcaa aaccaaagac ttggcacgcg
      361 tggaagtcat gttcaccgcc gactatctcg attatcaggc cgaatgggcg cgccgctacg
      421 aaggcgaaat catccaaagc gaccgcccgc gcgaaaatat tttattgttc aaacgtccgc
      481 tgggcgtaat tgccggcatt ttgccgtgga acttcccctt cttcctgatt gcccgcaaaa
      541 tgggccccgc tttggtaacg ggcaacacca tcgtcgtcaa acccagcagc gtaaccccga
      601 tcaactgcca catcttcgcc gaaatcgtcg atgcggtcgg actgcccgca ggcgtgttca
      661 acgtggtgaa cggtcccggc gcggaaatcg gcaatgcctt gtccgcccat ccgcaagtcg
      721 atatggtcag cctgaccggc tccgtcgaag caggccgcca agtgatggaa gccgcctccg
      781 ccaacatcac caaagtttcg ctggaactcg gcggcaaagc gcctgccatc gttttgaaag
      841 atgcggattt ggacttggcg gtgaaatcca tcttggcttc gcgcgtcggc aacaccggtc
      901 aaatctgcaa ctgcgccgag cgcgtctatg tccacagcag tctgaaagac gcattcattg
      961 aaaaaatgac cgccgcgatg aaaggcgtgc gctacggcaa ccctgccgaa gccgaagcag
     1021 gcgcgctgga aatgggcccg ctgattgaag aacgcgccgt caaagccgtt gccgaaaaag
     1081 tggaacgggc agtcaaacaa ggtgcgaaat tggtttgcgg cggcaaacgc gccgaaggac
     1141 gcggttattt cttcgagccg accctgctga ccgacaccga caacagtatg gacattatga
     1201 aagaagaaac cttcggccct gtgctgcccg tttccgcttt cgacacgctc gaccaagtca
     1261 tcgccttggc aaacgattgc gagtttggtc tgaccagttc tgtttatacg actaatttaa
     1321 acgaagcctt ctacgttacc cgccgcctgc aattcggcga aacctacatc aaccgcgaaa
     1381 actttgaagc gatgcagggt ttccacgccg gttggaaaaa atccggtatc ggcggcgcgg
     1441 acggcaaaca cggtttggaa gaatatctgc aaacccaagt cgtttatttg gaaaccgaca
     1501 tttaatgccg ctttaaaacc ccgatagaaa atgccgtctg aacccgtttt caggttcaga
     1561 cggcattttt attgcttcac cggcaatcag tcatgaccga ggtcgatgtt tttgtctttg
     1621 tatag
//
LOCUS       DAWIVB010000244         1585 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWIVB010000244 DAWIVB010000000
VERSION     DAWIVB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.244"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            152..1090
                     /locus_tag="WIX60_002007"
     CDS             152..1090
                     /locus_tag="WIX60_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ5306210.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            1219..1512
                     /gene="hfq"
                     /locus_tag="WIX60_002008"
     CDS             1219..1512
                     /gene="hfq"
                     /locus_tag="WIX60_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ5306211.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
BASE COUNT          473 a          479 c          304 g          329 t
ORIGIN      
        1 ttccgattta aaaacagaat gttataaaac ccaatcccca aaacacaaca agaccgcccg
       61 cctacgggca gtctcctgtc agacgacata ctttacagat ggctgttttt tcaacaaaat
      121 aacgccaata ctcaaaaata tggaatcaaa aatgtccatc catactctga aacgcctgcc
      181 ctcatcgctg ctgctcggtc tctgcctttc cctgccgtca gcccaccttt ttgccgacaa
      241 cgacatttta gggcaatttt tagaacagaa catgcttacc tcctccgatc cgatagaaat
      301 attcgccgaa agcacgatac accccaccaa cacccaagcc attacaggcg gtctgattct
      361 ctcctcacaa tctgccctgg tcgtcaacaa caaaaccgga cagatactgt atcagaaaaa
      421 cgccgacagg attatgccca tcgcctccat ttccaaactg atgagcgcga tggtcgtttt
      481 ggatgcaaac ttggacatga acgaaaccgt taccattacg cccgacgaaa tcgaccgcat
      541 caaagggacc ggcagccgtc ttgccatagg tacggcactt acacgcaaag aactgctgca
      601 cctgagcctg atgagcagcg aaaaccgcgc cacccatgca ttgggcagaa cctaccccgg
      661 cggcatgggc gcatttgtcg ccgccatgaa ccgcaaagcc caaagcctcg gtatgtacgg
      721 cagccgcttt tacgaaccga ccggactcaa cttccaaaac gtttctaccg ccaaagactt
      781 gagccttatg gtcaacgccg ccgcccaata tccgcaaatc cgcaccaact cgacttccaa
      841 ctacgcctcg gtacagacca aaaacgggca gcagaactac aaaaactcca atgccctggt
      901 cagagaaggc atgtggaaca tcgaattgca gaaaaccggc tacatacgcg aagcaggcag
      961 gtctatggtt gtcaaagcca acattcaaaa tcaacccgtt accatcgtat tgctgaactc
     1021 gcccacatcc gccacacgcg tcaacgacgc ccgcaaaatc gaatcgtgga tgctgcagca
     1081 acgctcctga catacaaatg cccggcggaa aaccgcccaa acacaaaaaa aattggtaat
     1141 ttttcctatt caaggctaca attcgacacg cacagggcat ctgcccgatt tcacaatcat
     1201 aatagcggag ttaaaaatat gacagctaaa ggacaaatgt tgcaagatcc cttcctgaac
     1261 gccctgcgta aagagcatgt tccggtttcg atttacttag ttaacggtat caaattacaa
     1321 ggtcaggttg agtctttcga tcaatacgtt gttctcctga gaaacacttc cgtcacccaa
     1381 atggtttaca aacacgccat ttccaccatc gtaccggcac gctccgtcaa cctacaacat
     1441 gaaaacagac cccaagccgc accgacttcg accctcgtcc aagtggaaac cgtccagcag
     1501 cctgccgaat aatccgcacg aagcatgacg tgtcatatct ttcaatacct taccggacaa
     1561 cggtaaggta tttttatttt cagac
//
LOCUS       DAWIVB010000245         1577 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWIVB010000245 DAWIVB010000000
VERSION     DAWIVB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.245"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            62..1435
                     /locus_tag="WIX60_002009"
     CDS             62..1435
                     /locus_tag="WIX60_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ5306212.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          403 a          524 c          387 g          263 t
ORIGIN      
        1 ttccccgtcc tatcggtttc ccgtttcaga cgacataagg tctgaaagaa agactacaat
       61 tatgagtaat ccattttcct ctttaggttt gggtacggaa ctcgtttccg cactgaccgc
      121 gcaaggttac gaaaacccga cgcccatcca agccgccgcc attcccaaag cactcgccgg
      181 tcatgatttg ctagccgccg cgcaaaccgg cacaggcaaa accgccgcct ttatgctgcc
      241 cagtctggaa cgcctcaaac gttacgccac cgccagcacc tcgcccgcga tgcaccccgt
      301 gcgtatgctc gtcctcaccc ccacgcgcga acttgccgac caaatcgacc aaaacgtgca
      361 gggctacatc aaaaacctgc cgctgcgcca caccgtcttg ttcggcggta tgaatatgga
      421 caaacagacc gccgacctgc gtgccggctg cgaaatcgtc gtcgccaccg tcggacggct
      481 gctcgaccac gtgaaacaga aaaacatcca tttgaacaaa gtcgaaatcg tcgttttgga
      541 cgaagccgac cgtatgctgg atatgggttt tatcgacgac atccgcaaaa tcatgcagat
      601 gctgccccgc caacgccaaa ccctgctctt ttccgccacc ttctccgccc cgatacgcaa
      661 actggcgcaa gacttcatga acgcgcccga aaccgtcgaa gtcgccgcgc aaaacaccac
      721 caacgccaac gtcgagcagc acatcatcgc cgtcgatacc attcagaagc gcaacctgct
      781 cgaacggctg attgtcgatt tgcatatgaa ccaggtcatc gtgttctgca aaaccaaaca
      841 aagcgtcgac cgcgtaacgc gcgaactggt gcgccgcaac ctgtccgcac aggcgataca
      901 cggcgaccgt tcccaacaaa gccggctcga aacactcaac gccttcaaag acggcaacct
      961 gcgcgtcctc gtcgccaccg acatcgccgc gcgcgggctg gacattgccg aactgccctt
     1021 cgtcatcaat tacgaaatgc ccgcccagcc cgaagactac gtccaccgca tcgggcgcac
     1081 ggggcgcgcg ggcgcggacg gcgtggcgat ttccctgatg gacgaatccg aacagaaaat
     1141 gtttgaatcc attaaagagc tgaccggcaa caagctgctc atcgagcgca tcgagggctt
     1201 cgagccgcaa tggtgggaac agggcggcgc aaaaccggaa aaacccgaaa tgcgcgaacc
     1261 gagacaacgc aaccgctacg aatccgccaa agcgcaacgc gaaaaaaaca cccggccgga
     1321 aaatgcggca aacgatgcgg gcgcggcttg cggaaaaatt gccggacgca gccgccgaag
     1381 ccgccgggaa caccggacgt gcgccctgct ccaaccgcgt tacggcgtaa aatagccctg
     1441 aaaatcaaat gccgtctgaa catttcccgt ttcagacggc atttttcaaa ccggactgac
     1501 gcatcgggag caaccgcccg caccggataa atttctgccg caaacagttt cagacggcat
     1561 ttgccgcctg tacaata
//
LOCUS       DAWIVB010000246         1549 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWIVB010000246 DAWIVB010000000
VERSION     DAWIVB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.246"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..187
                     /locus_tag="WIX60_002010"
     CDS             <1..187
                     /locus_tag="WIX60_002010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306213.1"
                     /translation="KSQTEKSQTENSETENSETENSETENSETENRKKPNRKQEKAKQ
                     KKACLATGFLLIPIFAD"
     gene            complement(184..1179)
                     /gene="porB"
                     /locus_tag="WIX60_002011"
     CDS             complement(184..1179)
                     /gene="porB"
                     /locus_tag="WIX60_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ5306214.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVFHQNGQV
                     TEVTTATGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHHQVQEGLNIEKYQIHRLVS
                     GYDNDALYASVAVQQQDAKLTDASNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGLV
                     DDADIGNEYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVATAGGVGLRHKF"
BASE COUNT          409 a          346 c          395 g          399 t
ORIGIN      
        1 aaaaagccaa acagaaaaaa gccaaacaga aaacagcgaa acagaaaaca gcgaaacaga
       61 aaacagcgaa acagaaaaca gcgaaacaga aaacaggaaa aagccaaaca gaaaacagga
      121 aaaagccaaa cagaaaaaag cctgtctggc gacaggcttt ttgttgatac caatctttgc
      181 agattagaat ttgtggcgca gaccgacacc gccggcagtc gctacgaatt tgttttcgcc
      241 tttgccttct tgcaaccaac cggcagaaac caaggcagaa gtgcgtttgg agaagtcgta
      301 ttccgcaccg acaaccactt ggtcgtattc gttgcctatg tctgcatcat caaccaaacc
      361 tttgaagccg tgggcgtaag aaactcgggg cgttacgttg ccgaagcggt atgccaaggt
      421 agcggcaact tcggtttgag agttgtgcga attggaagca tcagtcagtt tcgcgtcttg
      481 ttgctgtacg gctacggaag cgtacagggc atcattgtcg taaccgctga ccaaacggtg
      541 aatctggtat ttctcaatat tcaagccctc ttgcacttga tgatgtcttt tataggcacc
      601 gccatattgc acgaagaagc caccgttttt gtagttgaag ccggcgtggt aagattcgct
      661 gttatgtctg cctgcattgt cgttaagcgc gtattgtacg ctgccgctga ggccggcaaa
      721 ttcgggagaa tcgtagcgta cggaaatgag gcgtgcctcg ggttcggcaa ttttgtttac
      781 acccaaatag tcgcttttgc tatcccaagg attgatgtcg ccggtgtctt tcaggacgct
      841 gttcaaacga ccgacgcgca atttaccgaa gccgcctttc aagccgatga aggattggcg
      901 gttgccccaa ccggagtcag taccggcgat agatgctttt tgctcaacct gccaaatggc
      961 tttcaggccg ttaccgaggt cttcttggcc tttgaagccg attttcgaac ccaaatcaac
     1021 gatgccggta gcggttgtaa cttcagtaac ttggccgttc tggtgaaata cagagcggga
     1081 agtttctacg ccggctttga tggtgccgta cagggtaacg tcagccattg ctgcaacagg
     1141 aagggctgcc aaagtcaggg caatcaggga ttttttcatt gctgtattcc ttttttggtt
     1201 aagaaattta agccggccgg gctttccaag ccgcttagct ttgcatttac cgccgacgtt
     1261 ttcggcgatt ccatgtctga aactatagat gttcctgtct gaaaaaacaa acttctgcct
     1321 gtttttgtgt ggaattcgat gtgtattttg aagggcggat agagatatta tgggtatttt
     1381 ttgttttata acatatggtt atttaaattt tttaagattt gcatttttac aacacttact
     1441 cgggagggta ttggagggca ttgcaaaccg ggggttataa agacggcaaa aaacgcgcac
     1501 gtggtttctt tcggaacggg cggatgcaaa acccgcccct gcggcagga
//
LOCUS       DAWIVB010000247         1540 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWIVB010000247 DAWIVB010000000
VERSION     DAWIVB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.247"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            33..1433
                     /locus_tag="WIX60_002012"
     CDS             33..1433
                     /locus_tag="WIX60_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ5306215.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          410 a          490 c          367 g          273 t
ORIGIN      
        1 tatccaccgt ccgccttttt acggaaaccg aaatgacccc ttccgcactg aaaaaaaccg
       61 tcctgctgct cggcactgcc tttgccgccg catccgtcca cgcatccggc taccacttcg
      121 gcacacagtc ggtcaacgcg caaagcacgg caaatgccgc cgccgcagaa gccgccgacg
      181 catcgaccat cttctacaac cctgccggcc tgaccaaact cgacagcagc cagatttccg
      241 tcaacgccaa catcgtgctg cccagcattc attatgaggc ggattccgcc accgacttta
      301 ccgggcttcc cgtccaaggt tcgaaaagcg gcaaaatcac caaaaccacg gtcgcgcccc
      361 acatctacgg cgcatacaaa gtcaacgaca atctgaccgt gggcttgggc gtgtacgtcc
      421 ccttcggctc tgccaccgaa tacgaaaaag attccgtgtt gcgccacaac atcaacaaac
      481 tcggtctgac cagcatcgcc gtcgaacctg tcgccgcgtg gaaactcaac gaccgccatt
      541 ccttcggcgc aggcatcatc gcccaacata cttccgccga actgcgcaaa tatgccgact
      601 gggggattaa gagtaaagca gagatattga cggcaaaacc gcccaaacct aacggtgtag
      661 ccgaagctgc aaaaattcag gccgacggac acgccgatgt caaaggcagc gattggggct
      721 tcggctacca actggcgtgg atgtgggaca tcaacgaccg tgcgcgcgtg ggcgtgaact
      781 accgttccaa agtctcgcac acgctcaaag gcgatgccga atgggcggca gacggcgcgg
      841 cggcgaaagc aatgtggagt acgatgcttg cagcaaacgg ctacacggcg aatgaaaaag
      901 cccgcgttaa aatcgttacg cctgagtctt tgtccgtaca cggtatgtac aaagtgtccg
      961 ataaagccga cctgttcggc gacgtaactt ggacgcgcca cagccgcttc gataaggcgg
     1021 aactggtttt tgaaaaagaa aaaaccgtcg tcaaaggcaa atccgaccgc accaccatca
     1081 cccccaactg gcgcaacacc tacaaagtcg gcttcggcgg ttcttatcaa atcagcgaac
     1141 cgctgcaact gcgcgccggc atcgcttttg acaaatcgcc cgtccgcaac gccgactacc
     1201 gcatgaacag cctacccgac ggcaaccgca tctggttctc cgccggtatg aaataccata
     1261 tcggtaaaaa ccacgtcgtc gatgccgcct acacccacat ccacatcaac gacaccagct
     1321 accgcacggc gaaggcaagc ggcaacgatg tggacagcaa aggcgcgtct tccgcacgtt
     1381 tcaaaaacca cgccgacatc atcggtctgc aatacaccta caaattcaaa taaacggccc
     1441 gccgtttgaa tgtaacaatg ccgtctgaaa cagatttccg tttcagacgg cattgtttta
     1501 ggctcgattt atagtggatt aacaaaaatc aggacaaggc
//
LOCUS       DAWIVB010000248         1492 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWIVB010000248 DAWIVB010000000
VERSION     DAWIVB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.248"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(161..532)
                     /locus_tag="WIX60_002013"
     CDS             complement(161..532)
                     /locus_tag="WIX60_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306216.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMASKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIEIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(664..1107)
                     /locus_tag="WIX60_002014"
     CDS             complement(664..1107)
                     /locus_tag="WIX60_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306217.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1195..1419)
                     /locus_tag="WIX60_002015"
     CDS             complement(1195..1419)
                     /locus_tag="WIX60_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306218.1"
                     /translation="MMKNKYILIKAAISITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
BASE COUNT          478 a          274 c          223 g          517 t
ORIGIN      
        1 atccactata tatatccatt catatagaga aaaattctta tacccaatat aaaacaaggt
       61 ctttcattat attttgaaaa acctagaagc tctgtatcta ttggtttatc aaaattaata
      121 attattgcag aagacatgta ggctaataat cttgatttat ctatatttta ttaattatat
      181 atttaacccc gaagtcaata tagaaattta gtgattttaa agttctctta tcaatttcaa
      241 tctcagggag tacataaaaa tcctcagaat aggcaaactc tatatatgca tcatcattcg
      301 ataataattc aataatattt ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac
      361 atttattttt agatgccatt tttgaatatt gattaaataa ttttggatta ctatttttag
      421 taattaatcc ttccggagta ataaaaccac tttgtataaa gtcatcactt atattaacag
      481 ccttccgaaa aaaaatataa tcacactctt tgttatcaat ataaaatctc atactcttta
      541 ataatgaaat ataagaattt ccagaattac aagcaatttg aaaaaacatc attactccta
      601 aaactcataa ataataagga atatatatat gcatattcct tactattttt tcacaaatta
      661 tgcttatcta tgcttatttc cataaaaagc atgccctcga attttttgac ttgagttctg
      721 gaaatgagaa taatttgaat ggatttcctt ctgcccatgt ctaataacat tttgtcttcc
      781 ccaagcttgg tttgctaccg ctcttgcttg ttgagatgtt cgtgcataga cattagctcc
      841 tcttgataaa gcttgtgctg caacacgctg cgtaacccaa cgctgcgcaa ggaatctgcc
      901 cgcatgcaaa attcctattg ccaccaacgg tgccgcagcc ccttcagttt ctttcatctc
      961 cttttgagaa agctccgcca actgcatcgg cgcatctgcc cgcgtgtgga acacttggtc
     1021 ttcaaatgcc tgattgtcca atccgtttgc cattgcgggg gcgattatag acagcatcat
     1081 cgtggcttca gtgatttgtt ttttcataac aaattcttag ggaggagata atgttccatc
     1141 aaaatcctcc tgaaaggctg cctgaaattt ccaagcagcc tttttgattg cttattaatc
     1201 ggatagtgcg acaaatataa agtgggcggc aatcattgcc gtaacggcag ccataactgt
     1261 tttattttct tcaaaccaaa gcaagttgag aaacataaac atcaagaaat atgatggttc
     1321 ttcagttttg ccgctgcttt cttgagttag aagataaaat atagatatgg cggttatgct
     1381 gattgctgct tttattaaaa tatatttgtt tttcatcatc ttgtatggtt tttcatgcaa
     1441 gtttcaacaa taaaattcgg aataagatag agagatgttg atatatcaga ta
//
LOCUS       DAWIVB010000249         1448 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWIVB010000249 DAWIVB010000000
VERSION     DAWIVB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.249"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            128..1258
                     /locus_tag="WIX60_002016"
     CDS             128..1258
                     /locus_tag="WIX60_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ5306219.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGGDLERLAARDIIRFPGFVNNVSEVIKEH
                     HIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          384 a          344 c          343 g          377 t
ORIGIN      
        1 ggcaacgccg taccggtttt tgttaatcca ctataatacg caggcaaacc caagccttgc
       61 tgggttacag gatgaaaccc tgccgtagca gaccgcattt ccaatccata ttcaaggtgt
      121 ccgccttatg aaaatcgttt ttatcacaac agtcgcatcc agcatttacg gtttccgcgc
      181 ccccgtcatt aaaaaattaa tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt
      241 ttccgacaat gaattggata ttatcaggga aatgggggtt acacccgtta cctaccgttc
      301 aaaccgcagc gggctgaacc cgttttcgga tataaaatcc accttcctca tctttaaaga
      361 actcaaaaaa atatcgccgg atttggtttt cccttatttc gcaaaacccg tgattttcgg
      421 cacttttgcc gcaaaactgg caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg
      481 attcgcattt accccgcagc cggaaggcat accgttaaaa acaaaaatca taaaggggat
      541 tttgattgcc ttataccgca ttgccctgcc gatgttggaa agcctgattg tattaaaccc
      601 cgacgacaaa gacgaactga cggacaaata cggcatcaaa ataaaaaaca tccatatttt
      661 gggcggaatc ggtctggatt tgcggcaata tccttattcc gaggcggata ttcccgatga
      721 aaaagaaccc gtaaaattcc tctttatcgg cagatttctg aaagaaaagg ggattgatga
      781 ttttattcgg gcggcggaac aggttaagga caaatacccc gatacggttt ttaccgcttt
      841 gggcgcaatc gacaaatcac gcgggggggg gggcgatttg gaacggcttg ccgcccgcga
      901 tattatccgt ttccccggtt ttgtgaacaa tgtttccgaa gtgataaaag aacatcatat
      961 attcgtattg ccgtcttatt atagggaagg cgttccccga agcactcagg aggcaatggc
     1021 cgtcggcagg gcagtgatta cgacggatgt ccccggatgc agggaaacgg tcgccgacaa
     1081 ggtcaacggc ttcctgatcg agccttggaa tccccgcatc ttggccgaaa aaatgattta
     1141 ttttatcgaa aacagggaag ccgtccgcct gatggggaat gcaagttatg cgattgccaa
     1201 agataaattc gatgccgaaa aagtcgattt gaaattgctc gatattttga aggcgtaaac
     1261 aaggctgccc gcttttgagt ttcggtcatt tctgataaac ccccgtcatt cccgccacac
     1321 ccccgtcatt ccctcgaaag cgggaattca ggttcgttta gtttcggtca tttccgataa
     1381 attcttgcaa ctttgcgttt ctagattccc actttcgtgg gaatgacggc ggagggttgc
     1441 tgttttcc
//
LOCUS       DAWIVB010000250         1443 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWIVB010000250 DAWIVB010000000
VERSION     DAWIVB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.250"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            27..1364
                     /locus_tag="WIX60_002017"
     CDS             27..1364
                     /locus_tag="WIX60_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ5306220.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIIGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          263 a          349 c          380 g          451 t
ORIGIN      
        1 taccttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtcct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttactatct
      181 tggtcgccct acccgttcag cttgccgaat tttatatcgg gcgcacgggc ggtaaaaatg
      241 ccgtcgattc cttcagggtt ctgcgtccgg gcacgcaatg gctttgggtc gggcgtatgg
      301 gcgttgccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattaa
      361 attatgtcgt ccacagtttt acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtttg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggc caggcgtttt ttgccctgag catcggcgtt tccgccatga
      721 ttacctacgc ttcatatttg ggaaaagatc aggatatgtt ccgttccggc catacgatta
      781 tgtggatgaa cctcttggtt tcgctgcttg ccggcctggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg cccgcagtgt
      901 ttatgaagat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat tgcctcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa cacacttggc ttatcggcac ggccattttc attatcggca
     1081 tcccgtccgc gctgtctttc ggcgtatggg gcgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactatgtt atttccgccg tcattatgcc gattggtgct ttgagtgttt
     1201 ccatctttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac cttgccccga
     1321 ttgccattat tattgttttc atcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcgggttcag cctatttcgt
     1441 tca
//
LOCUS       DAWIVB010000251         1371 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWIVB010000251 DAWIVB010000000
VERSION     DAWIVB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.251"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..>1371
                     /locus_tag="WIX60_002018"
     CDS             <1..>1371
                     /locus_tag="WIX60_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306221.1"
                     /translation="LRAFAEKGYVGDRYGVDGGFVLRQVELSGQKHVFMFGAMGFGGR
                     GAYALDLTKADGNDPTKASLFDVKDNGNNGNNGNNRVELGYTVGTPQIGKTHDGKYAA
                     FLASGYATKTIDDQQNKTALYVYDLESSGTLIKKIDVPGGKGGLSSPTLVDKDLDGTV
                     DIAYAGDRGGKMYRFDLSSQSPDQWTVRPIFEGTKPITSAPAVSKLKDKRVVIFGTGS
                     DLSEEDVDKMDEQYIYGIFDDDTAATGTVNFSGSGGGLLEQVLSRDNDNKTLFLTDYK
                     RSDGSGSKGWVVKLEAGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAETAILGINTADG
                     GKLTKKSARPIVPEANQAVAQYSGHKQTAKGKSIPIGCMEKNGGTVCPNGYVYDKPVN
                     VRYLDEKKTDGFSTTADGDAGGSGIDPDGKRAGKNNRCFSQKGVRTLLMNDLDSLDIT
                     GPTCGM"
BASE COUNT          373 a          341 c          387 g          270 t
ORIGIN      
        1 agctgcgcgc ctttgccgaa aaaggctatg tgggcgaccg ctacggcgtg gacggcggct
       61 ttgtcttgcg ccaagtcgaa ctgagcgggc aaaaacacgt gtttatgttc ggcgcgatgg
      121 gctttggcgg cagaggtgcg tatgccttgg atttgaccaa agccgacggc aatgacccga
      181 caaaagcctc tttgtttgat gtaaaagata acggcaataa tggcaataac ggcaataatc
      241 gcgtggaatt aggctacacc gtcggcacac cgcaaatcgg caaaacccac gacggcaaat
      301 acgccgcttt cctcgcctcc ggttatgcga ctaaaaccat tgacgaccaa caaaataaaa
      361 ccgcgctgta tgtgtatgat ttggaaagca gcggtacgct gattaaaaaa atcgatgtac
      421 ccggcggcaa gggcgggctt tcgtccccca cgctggtgga taaagatttg gacggcacgg
      481 tcgatatcgc ctatgccggc gatcgcggcg ggaaaatgta ccgctttgat ttgagcagtc
      541 aaagtcctga tcaatggact gtacgcccta tttttgaagg cacaaaaccg attacttccg
      601 cgcccgccgt ttccaaactg aaagacaaac gcgtggttat cttcggcacg ggcagcgatt
      661 tgagtgagga ggatgtggac aaaatggacg aacaatatat ttacggtatc ttcgacgacg
      721 atacagcggc gacgggtact gtaaacttca gcggttcggg aggcgggctg cttgagcaag
      781 tgcttagtag ggataacgac aataaaacct tattcctgac cgattacaag cgatccgacg
      841 gatcgggcag caaagggtgg gtagtgaaat tggaagccgg acagcgcgtt accgtcaaac
      901 cgaccgtggt attgcgtacc gcctttgtaa ccatccataa atatacgggt acggacaaat
      961 gcggcgcgga aaccgccatt ttgggcatca ataccgccga cggcggcaag ctgaccaaga
     1021 aaagcgcgcg cccgattgtg ccggaagcca atcaggctgt cgcgcaatat tccggccata
     1081 agcaaaccgc caaaggcaaa tccatcccta taggttgtat ggaaaaaaac gggggaaccg
     1141 tctgcccgaa cggatatgtt tacgacaaac cggttaatgt gcgttatctg gatgaaaaga
     1201 aaacagacgg attttcaaca acggcagacg gcgatgcggg cggcagcggt atagaccccg
     1261 acggcaagcg tgccggcaaa aacaaccgct gcttctccca aaaaggggtg cgcaccctgc
     1321 tgatgaacga tttggacagc ttggacatta ccggcccgac gtgcggtatg a
//
LOCUS       DAWIVB010000252         1330 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWIVB010000252 DAWIVB010000000
VERSION     DAWIVB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.252"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..974)
                     /locus_tag="WIX60_002019"
     CDS             complement(<1..974)
                     /locus_tag="WIX60_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ5306222.1"
                     /translation="MYALSEKYNDNGFKAYKVLGEGGGIHTEYNYKFDKSLNLNVLES
                     STGARSLEKVPVKVTASVSRAAVLSGVGKLARLGAKLSTRAVPYVGTALLAHDVYETF
                     KEDIQAQGYQYDPETDKFVKGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSR
                     FPEVKELMESQMYRLARPFWNWHKEELNKLSSLDWNNFVLNRCTFDWNGGDCVVNKGD
                     DYRNGANFSLSRNPKYKEEMDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPG
                     TKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEA"
BASE COUNT          382 a          282 c          230 g          436 t
ORIGIN      
        1 gcttccgcgc ttccgggtgt gaggtcggga cgcgggatta cttgaacatc cactgtggtg
       61 ttgccttgcg aatccctgcc gaatgttgcg acaacctgaa cgggattccc gttcctgtcc
      121 gtaacaggcc ccatattcac ttttgttccg ggtgctactt ctactttttc ggaataaccg
      181 ggatatccgg ttgcctttat gtatttgtcg ggattggcat cgactttcaa cgataaaatc
      241 tcttccagct ttttggcatc catttcttct ttgtatttcg gattgcggct aagggaaaaa
      301 ttagccccat ttctgtaatc atcaccttta ttgaccacac aatctccgcc gttccaatca
      361 aatgtgcaac gatttaaaac aaaattattc caatccaaag aacttaattt attcagttct
      421 tctttatgcc aattccaaaa cggacgtgcc agcctataca tttggctttc catcaattct
      481 ttgacttcgg ggaatctgct gtaatcggac ataaggcgca taatcgaact gtcaacgccg
      541 tagcagccat aggttctatt aatacgtctt tcgtcttcat accaaaggca attactatat
      601 tcgtagcctt ttacaaattt gtcggtttcg gggtcgtatt ggtagccttg tgcctgtatg
      661 tcttctttga aagtttcgta tacgtcgtgg gctaaaaggg ctgttccgac ataaggaact
      721 gcccttgtgc ttaatttcgc gcctaagcgg gcaagtttgc cgactcctga caagacggcg
      781 gcgcgggaaa ctgatgcagt tactttaacg gggacttttt caagagagcg tgcgcctgtt
      841 gaactttcta atacattcaa attcaaactt ttatcaaatt tgtaattata ttctgtatga
      901 attcctcctc cctcacctaa aactttgtat gccttaaatc cattatcgtt atatttttcc
      961 gaaagtgcat acatcaattt cccattttta atttctaaat ctgctgaaaa agatttagca
     1021 ctcaaaagaa acagaaaaga aattattaga atccgaaaca tcaatttttc cattgccaat
     1081 aatgaaaaat gaatcatcct tgaatttaat ttcaaagcaa gattcattaa aattaattct
     1141 attaaaaaaa ttatgacatt tatccattga gaaatttttc aatagaccag tttctttcaa
     1201 ataacaataa aacacataag aaagaggcct atcaggataa gattctgaca atttatctaa
     1261 atcagaattt gatatataga aaaagtcacg ttcattttta ttcatggttt caagcctcaa
     1321 atgtctaatt
//
LOCUS       DAWIVB010000253         1326 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWIVB010000253 DAWIVB010000000
VERSION     DAWIVB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.253"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            371..619
                     /locus_tag="WIX60_002020"
     CDS             371..619
                     /locus_tag="WIX60_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306223.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
BASE COUNT          414 a          190 c          275 g          447 t
ORIGIN      
        1 cacctgcgac aaatagctca atgagtttat tttgtttata ctggcttaga cgactttttc
       61 tcatagggat aattctaact taatttgaat ttccctagtt atctaggaca gccccaaagt
      121 ttattgtctt taaatgagat aacagataaa accttattta aagaaaatgt agtttatcgt
      181 ttcagtaagt attaggtgtg atttttatta agttatttga ggttttcggt aagtgtagat
      241 ataaggttga gttataacca atcattcagg ctctgttagg cttggcaact caacctgtgc
      301 ttgctgaaat tgggtataca gttatacgtt ttaagcgggc ttcgctccct ttatcattta
      361 agggtaattt atgaatatta aaaatgaaat agttgcttta atttctaaaa aaattgatgg
      421 ggggcaatat agttctttag aaaattttgc tttatccgta ctaagggaaa aaattataaa
      481 aaatactgtt tccagtgatg aaaaattatt gattattaat gattttttat caggttttct
      541 tgagtttgat cctgagaccg ggaaacctgt cggggagacg cttaaaattg agcaaatgat
      601 tgatttcttt ttagtctaaa tcatacatac cagtgaacaa gctaggctta tatccggctt
      661 ttaaatacga ttgtcaggtt tatacttggg ataaaatcaa aaataaagtt gtttaaactc
      721 tagtagatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacgg
      781 aaccgattca ctcggtgctt caacacctta gagaatcgtt ctctttgagc taaggcgagg
      841 caacgccgta ctggtttttg ttaattcact atattgcatg gtgcagcatt aaatcagtct
      901 tctagcgccg caatgggaaa acatagaaga taaacgaaat ggatgatcta atactatatt
      961 ttttatcagg tatattcgga aatcaagttg ctgaatgtat tataaaaaac aaccgtgaga
     1021 taaaagttcc ctttattgtg ctttacgcaa tattttttac gttgatttat actgtggctt
     1081 tgttgtttct cagtctgatc tattgggtca atggtgcgga gattgcatgg aaggggatag
     1141 gtattttcag tatgtcggtc agtttttgta tagtcttctg tctttatttg attgacaagg
     1201 caggaagatg taaggataag aaacaatagg gatcagatgc ggcagcaaac gtagattggg
     1261 tctaggctca acctgcgttt gctgcatctt tggaagaaat agtggattcg aaaaaatagg
     1321 aagaat
//
LOCUS       DAWIVB010000254         1269 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWIVB010000254 DAWIVB010000000
VERSION     DAWIVB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.254"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            41..373
                     /locus_tag="WIX60_002021"
     CDS             41..373
                     /locus_tag="WIX60_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ5306224.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            705..1214
                     /locus_tag="WIX60_002022"
     CDS             705..1214
                     /locus_tag="WIX60_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ5306225.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          340 a          241 c          302 g          386 t
ORIGIN      
        1 accttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 accggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatatgccgc cgggcattcc ctgtttttgg gtggaaggcg cggaaagcct
      241 tgaagagttg tgcgtcatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg acgcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggg gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgaaa tgggctgaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcagca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcgaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggagattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gcctgcccat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tagcggatta acaaaaacca gtacggcgtt
     1261 gcctcgcct
//
LOCUS       DAWIVB010000255         1258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWIVB010000255 DAWIVB010000000
VERSION     DAWIVB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.255"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(85..1188)
                     /locus_tag="WIX60_002023"
     CDS             complement(85..1188)
                     /locus_tag="WIX60_002023"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ5306226.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERASILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
BASE COUNT          285 a          349 c          341 g          283 t
ORIGIN      
        1 tttccgacag ccgaacaaaa ccccgaatcc gtctgcacgg ttcggggtat atctccaata
       61 cgggcatcgt gttccggaaa accgtcaaat ccgcatcggc atcacaatat atttgaaatt
      121 cggattgttc ggcacggtaa acagcgtcga gcggttggca tcgccgaagg caagctgcat
      181 atcgtcggaa tggatgttgc gcaacacgtc catcagatag ccgatattga aaccgacttc
      241 gagttcgccg ccctgatagg cgatttcgat ttcttcgcgc gcttcttcct gctcgttgtt
      301 gctgcacaca acgctcaaca ggccgggttg caaaaacaat cgcgcaccgc ggaatttttc
      361 attggcaaga atcgatgcac gttccaacgc gcccaacaat tctgccctcg acaacacgaa
      421 aatcttgtcg ttgtccaaag gaatcacgcg gttgaaatcg gggaatttgc cgtcgatgac
      481 cttgctgacg atggtcgtgc cgttgcattg gaaacgcacc tgtttgtcca gcagctcgat
      541 ttgaatcgga tcgtcggggt tgttcaacag tttgaacagt tccagcaccg ttttgcgcgg
      601 caaaatcact tcggcgcgcg gcaaatccgc atcaatcgcg caggctgcat aggcaaggcg
      661 gtgtccgtcg gtcgccacaa ggcgcaactg gctgccctca acctgcatca gcagaccgtt
      721 gagataatag cggatgtcct gcaccgccat gctgtactgc acttgcgaca gcatggtttt
      781 gaaacgctcc tgctccagcg agaaagtcgc gctgatgtcc tcgccgacat tcatcatcgg
      841 aaaatcggcg gcaggcaggg tctgcagggc aaaacgcgat ttgcccgcct tcagcgtcag
      901 acggctgtcg tcccaatcca gcgacaccag cgcaccggca ggcagcgcgc gcaaaatatc
      961 ctgaaatttc ttggcattgg tggtgatgcg gaagtcgccc gcgccgccct cgggacccgc
     1021 agtgtcgatt tggatttcca aatcggttgc caagagtttg gtctgaccgc cttttccctc
     1081 aatcaggacg ttggacagga tgggcagggt gtggcggcgt tcgacgatgc cggtaacggc
     1141 ttgcaacggc ttgagcaggc tgtcgcgctc ggcttgtaaa atcaacatgt tcgctccttt
     1201 aaatcggttt gtatagtgga ttaaatttaa atcaggacaa ggcgacgaag ccgcagac
//
LOCUS       DAWIVB010000256         1221 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWIVB010000256 DAWIVB010000000
VERSION     DAWIVB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.256"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(34..492)
                     /locus_tag="WIX60_002024"
     CDS             complement(34..492)
                     /locus_tag="WIX60_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306227.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     SYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
BASE COUNT          436 a          222 c          128 g          435 t
ORIGIN      
        1 caatcgattc cgttgtattt tcgcaagaca cgtttagtct agagtctgta tattacattt
       61 atttttaggg tctgctagcc aatttcttgt tcccttcatt attttatctt ctgaaagaaa
      121 attatttttt tccatgctat taatattaat gatatgattt ttatttaaaa taatttttcc
      181 atatgaaatt tccggtttgt aatagcaaga gtctgaagca aatccttgcc cgtcaatata
      241 agaaaaataa atgttatcat cctccttata atattctaaa accaatccgg atattatttt
      301 atcaaaattc ccttcatttc catctttcat gactaaaata taattagcag gccaaccttt
      361 atcgtaaaca tattcaaact gatagttccc gaaactctcg atatccgaac taaaaaagaa
      421 gaaaagcaaa ttaatagcca atacaaaaac aaataagaac aaaaatagca aaattttcaa
      481 cttagttaac aatatttacc tctcctttaa attcaatcct gaaaggtacc ccttaccctg
      541 ggctaccaat tatagtttcc atatttctaa atattgtttt tacattactt ttttctcccc
      601 ccaaaggaat gcattttaaa atcatgcttt tcaggtgcta atcgatactt accattaaca
      661 tctttaatca cagatatatt tccatgtata gcacaacgtg aaaaatctga gtattatata
      721 cagttatacc ttactgaaac aaagctagct gtacatatct gtaccatatt tttactacaa
      781 acaaaaaata tttataatta ttaaggataa gataaaaacc cccaacgcca gattaattct
      841 taataatttc aaataaaaaa tatttgagat tttttctttt ccaaataata tattgaaata
      901 tttaaagtta aggtagcttg atttaattag aaaatcaaaa aaaccattaa ttaatttttc
      961 ttctttaaat attttatatg atattataaa attaaataac attgcgaaaa catatgaaat
     1021 catcaaaata ccaaatatat agtgggaatg cattattatt tagctccttc ttttggcttt
     1081 gacaaattga cagtccattg atatccaatg cttgcatttg caattgctga cgcacctgcc
     1141 cctgttttcc ctgttagtat caatgattct tttcttgcaa gatgtgcacc tgcacaacca
     1201 cctaatacta aaccaccacc t
//
LOCUS       DAWIVB010000257         1209 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWIVB010000257 DAWIVB010000000
VERSION     DAWIVB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.257"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            39..462
                     /locus_tag="WIX60_002025"
                     /pseudo
     CDS             39..462
                     /locus_tag="WIX60_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            568..1131
                     /gene="pgsA"
                     /locus_tag="WIX60_002026"
     CDS             568..1131
                     /gene="pgsA"
                     /locus_tag="WIX60_002026"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ5306228.1"
                     /translation="MPWNLPIFLTWLRVLLIPVLTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNFPDFYGFNLAFIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
BASE COUNT          289 a          290 c          296 g          334 t
ORIGIN      
        1 atcgctgggt atggtttgcc gccataagct gtctgctgtt gtccgccgtt tttattgcac
       61 cttacctgac ggcatttcac gaacaagaaa aaacattcga atatgcggaa ttgaccgtta
      121 ccgccctcaa ccgcagcgga cgggcaatca aactggatgc agacgggcag cattaccggc
      181 tttcctgcta tgggttcgac agtttgtgca caggcggcaa tatcggcaga accatcaggg
      241 cggagcaggt tagagccgtt ttaagcgaaa ccgtcggcaa aggtttttta aacggcgttc
      301 tgttggaata ccgcaacagt ggcagtgtct ataagcaata aagattttgc ccgcacggaa
      361 gaccgccttg tcgaagtgtt ggcacaacct gccgttttca gcctgaaact gggtattttg
      421 cttttattgc ccgccatttt cctgcgtttg aaaagaatgt gaaaacagat tggcggggcg
      481 ggggaatgcc catgcagcaa ttttacgttt tgggttatga ggataaccgt tataatcaca
      541 actttacagt ctgcacagag aaaaccgatg ccttggaatc tccccatctt cctcacatgg
      601 ttgagggtct tgctcattcc tgtcctgaca gccctttttt acctgccttt tccgtggttc
      661 gcggaggaaa cgattaatct caccgccgcc gtcatttttg ccgttgccgc cttgaccgac
      721 tggtttgacg gatttttggc aaggttgtgg aaacagacct cggatttcgg cgccttcctc
      781 gatcccgttg ccgacaagct gatggtcgcc gtctctttgc ttttactggt caaactcgac
      841 cggacctatg ttttgtttgc catgatcatc atcggcaggg aaattaccat ttccgcattg
      901 cgcgaatgga tggcgcaaat gggcaaaagg aacagcgttg ccgtcgccac cgtcggtaag
      961 tttaaaaccg ccgcgcaaat gctggcaatc ttcctgctgc tgctgaattt tcccgatttt
     1021 tatggattta acctcgcatt catcggcaat gtattgatgt ttatcgcatc tttgctgaca
     1081 gtctggtcga tgctgtatta tctgaaaatg gcgtggaaag aaatcgcctg aaaaaaacat
     1141 aaaaatagct tgacggcaaa aacagaatcc ataataattg tgtctcttcg atatcggaga
     1201 atgaaatcg
//
LOCUS       DAWIVB010000258         1208 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWIVB010000258 DAWIVB010000000
VERSION     DAWIVB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.258"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(179..538)
                     /locus_tag="WIX60_002027"
     CDS             complement(179..538)
                     /locus_tag="WIX60_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306229.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDN
                     YKVNWKINNGNLIQTTN"
     gene            complement(541..>1208)
                     /gene="mafB"
                     /locus_tag="WIX60_002028"
     CDS             complement(541..>1208)
                     /gene="mafB"
                     /locus_tag="WIX60_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ5306230.1"
                     /translation="GIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEK
                     NTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDFADSYKKKL
                     ALSDSARQLYQNAKYREALDIHYEDLIRRKTDGSSKFINGREIDAVTNDALIQAKRTI
                     SAIDKPKNFLNQKNRKQIKATIEAANQQGKRAEFWFKYGVHSQVKSYIESKGGIVKTG
                     LGD"
BASE COUNT          320 a          252 c          197 g          439 t
ORIGIN      
        1 aagaacttag ttctcaatat cctgtttcat tcatcaaaat gccgtctgaa agctgaatac
       61 cgcttcagac ggcattttgg tggttgggtt tttaagccaa cctacgctta ctgaaaacca
      121 aattgagttt cagacagttt ttaggtttgg gtgtccaatc taacttatat ttgtccattt
      181 aattagtcgt ttgtatcaaa tttccattat ttattttcca atttacttta taattatctt
      241 cataataatc taattcaaaa aaacctgata tttcaatatc caattccatt attgttttaa
      301 tacatttttc aaaataaata atgaaataag attttacgca tgcaccaaaa aaaatatagc
      361 tgctccaatt aaaactattt gtcgggaaaa cccacccgct tttatatatt tttgcagatt
      421 ctttctcttc gatattaaag ggacaattat tccaaaaatt attaatgttt tcttggatga
      481 tttttatatc atcgtcagag cattcaatcc atccttttat ggaaacatat gatgccatgt
      541 ttaatctcct aaacctgttt taacaatgcc gccttttgat tcaatatatg acttaacttg
      601 tgaatgaaca ccgtatttaa accaaaattc tgcacgtttt ccctgttggt ttgctgcttc
      661 gatggttgct ttaatttgct ttctattttt ttgatttaag aaatttttag gtttatctat
      721 tgctgaaatt gttcttttgg cttgtattaa agcatcattc gtaacagcgt caatttctct
      781 gccgttaata aattttgatg aaccatcagt ttttcttcta attaaatctt cataatgtat
      841 atctagagct tctctatact ttgcattttg atataactgt ctcgcactat cagacaaagc
      901 caatttcttt ttataagaat cagcaaaatc cccgctaacc gcagccttcc ctggttttgc
      961 cgcctttgcc aacttcgcga ctttggctgc tgcggcaacg ttgaagacgg cttcgacggt
     1021 ttcggcggca ttgggatttt cctgtatcca ccggtcaacg gcttcgcgcg tattcttttc
     1081 aaagcccgcc acgctgccca agccgccgat gacggcgaat ttgccctcgg cgggcaaggg
     1141 ggcgatgttg cgcattgcgg ctttgtctat ggcatagcgc gttccgtaca gtatgtcgcc
     1201 tatgccca
//
LOCUS       DAWIVB010000259         1164 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWIVB010000259 DAWIVB010000000
VERSION     DAWIVB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.259"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(<1..158)
                     /locus_tag="WIX60_002029"
     CDS             complement(<1..158)
                     /locus_tag="WIX60_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ5306231.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRP"
     gene            complement(220..891)
                     /locus_tag="WIX60_002030"
     CDS             complement(220..891)
                     /locus_tag="WIX60_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ5306232.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          295 a          253 c          278 g          338 t
ORIGIN      
        1 ggacggccgc ggtggtctct aaggtaacgg gttttttgac ggttcaggta ctgctcgatc
       61 ggctgccaat caatcacctg gtccaacttc aatagcggga aacggtcgat gtgtctggca
      121 atcatggctt gggcggtttg ctggaagaag gtgctcatgg aaaatcccct aaatgtcttg
      181 gtgggaattt aaggggtttt ggggaatttt gcaaaggtct cagtctatgc ccgatataca
      241 attttgatac acaaacttgg aaatatcggt atcgtcgccg gagcgataga atgcggacag
      301 tttttcattg aattgcggca tctgcatttc ggagatttcc aagatgccgc agccccccgc
      361 catcatcaga ccgttggcaa ataaggttgc cgttcgtttg ttgccgtccc aaaaaacctg
      421 ctgccgcata caataaagca taaaacggac ggctgcctcc gtcgtcgaac cgctttgcag
      481 tccgatattt tgtaacacgc gggccacttc aatttccttc actggattcg gggcatgacg
      541 ggaaccgtcc aatagcgtta cgccgaccga accggtacgg aaatcaccgg gtgccaaaga
      601 atcgtccttg gcaacaatgt tgttgatttt tttaaggagt gaaatatcga ccggttcgcc
      661 gtttgaaata tgcgaaatca catattgata ggcacgtttc aggttcaaga tggtttggat
      721 gtcttccagt gacgcggagg ctacattttt gccatggata atttgttcgg tctgcaacaa
      781 ggtcgtctga caattctcaa aacggctgag gttgtgaatt tgggcaacca atactttctt
      841 tgccaaaaaa atattttctt tcaaactcaa cttgtatttg tcgggaaaca cgataattcc
      901 ttaaataatt aaagcagtac gttaaatcat catcttatca tcactttcgg ggtttatgtt
      961 gcgtgcgtcc gttttctaat acggtttccc atcccgcgaa acgaggcgga ggcctgtgca
     1021 aaaaacctgt tcacgaaatt ttccgcatta acgatacgct gaaaatgcgc taaaaatgac
     1081 tgtgtttgaa tatcggttga tttcatccgt ttgtgtaacc tcgcgccggt tttgtcatga
     1141 ttttgcatta tagtgaatta aatt
//
LOCUS       DAWIVB010000260         1151 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWIVB010000260 DAWIVB010000000
VERSION     DAWIVB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.260"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            81..1046
                     /locus_tag="WIX60_002031"
     CDS             81..1046
                     /locus_tag="WIX60_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ5306233.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSEGASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNLLPYLDEYFKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNAIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT          290 a          321 c          303 g          237 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccct atgttgcgtt tgaccgcatt gaccctctgc accgtcctcg
      121 ccttgggcgc gtgttcgccg aaagattccg actctgcccc acaagccaaa gaacaggcag
      181 tttccgccgc acaatccgaa ggcgcgtccg ttaccgtcaa aaccgcgcgc ggcgatgttc
      241 aaataccgca aaaccccgaa cgcatcgccg tttacgattt gggtatgctc gacaccttga
      301 gcaaactggg cgtgaaaacc ggtttgtccg tcgataaaaa cctgctgcct tatttggacg
      361 aatatttcaa aaccacgaaa cctgccggta cgctgtttga gccggattac gaagcactca
      421 acgcttacaa accgcagctc atcatcatcg gcagccgcgc agccaaagcg tttgacaagt
      481 tgaacgccat tgcgccgacc atcgagatga ccgccgatac cgccaacctg aaagaaagtg
      541 ccaaagaacg tatcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc
      601 tgaaagcgga aatcgacgcg tcttttgaag ccgcgaaaac tgccgcacaa ggcaaaggca
      661 aaggtttggt gattttggtc aacggcggca agatgtcggc tttcggcccg tcttcacgct
      721 tgggcggctg gctgcacaaa gacatcggcg ttcccgctgt cgatgaatca attaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 ttgtccttga ccgaagcgcg gccatcggtg aagagggtca ggcggcgaaa gacgtgttgg
      901 ataatccgct ggttgccgaa acgaccgctt ggaaaaaagg acaggtggtt taccttgttc
      961 ctgaaactta tttggcggcc ggcggcgcac aagaattgct gaatgcaagc aaacaggttg
     1021 ccgacgcttt taacgcggca aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcctcctgtg tttcagacgg cattgcccga tacgggcgtt atctcgggaa
     1141 tggcggcaag c
//
LOCUS       DAWIVB010000261         1128 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWIVB010000261 DAWIVB010000000
VERSION     DAWIVB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.261"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(231..1085)
                     /locus_tag="WIX60_002032"
     CDS             complement(231..1085)
                     /locus_tag="WIX60_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ5306234.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          314 a          339 c          294 g          181 t
ORIGIN      
        1 tcccgtcatt cccgcgcagg cggaaatccg gacatccaac gctgcagcaa tttgtcggaa
       61 ataaccgaaa ctcaaaaaac cggatgcccg cctgcgcggg aatgacgacg caaagatggt
      121 cgttatttcg gataaatatc cgtcttttcg ttccggatac ccgctttcgc aggaatggag
      181 atggaaagat ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga
      241 tgcgacagcc ggccggcaac caaggtggcg gcgatgacga caaacacgcc caaggcggac
      301 acgggcgaca ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg
      361 agcgaaatca acagtcccga aacattggca ggaacacggc tcatcccctt gttccacagc
      421 caataggcgt accagccgca ccccaaaccc aaatacagca gcgacaatac catcccgacg
      481 ctccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
      541 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc
      601 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
      661 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
      721 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
      781 acaaacacca tcagcagcgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
      841 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc
      901 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
      961 gcaggcagcg cggcaattag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
     1021 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
     1081 aacatagtga gtgataaagt cagatggtca tacaaaagta tagcggat
//
LOCUS       DAWIVB010000262         1032 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWIVB010000262 DAWIVB010000000
VERSION     DAWIVB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.262"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(122..550)
                     /locus_tag="WIX60_002033"
     CDS             complement(122..550)
                     /locus_tag="WIX60_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ5306235.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KKSGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(531..965)
                     /locus_tag="WIX60_002034"
     CDS             complement(531..965)
                     /locus_tag="WIX60_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ5306236.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          262 a          316 c          190 g          264 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcacccc
       61 tgcaaaccaa aaaaaagacc gtctgaatac agacggccaa agcctggtca cacatcactg
      121 cctaaaataa agctgcctgc tgtgccgcag gtcggctcat ttcattgata atcgtatatc
      181 ccgttcgtga agagatgccg tatttagggc atagcttcgt catcgccata agcccgctct
      241 tcttatcaat atcgcgcaat ttgacaaact cctgataaaa cctatggttt ctcaactgta
      301 ttaacgcctt gccgcaccgt gggacataca attcctcgcc accatatacc tgcaacagct
      361 catgtgtttt cacttcgccg atggcttcga ccaaaattgc caaacgctcg gtgtccacct
      421 tgcccttacc aaatttaaac cgcgccccgc caatcgcctt gaccagctgt tccgtcgctg
      481 ccagtccgat gacatccaca atgtccaaca cggtatccgg caataaatgt tcaacttttt
      541 cgaaccccat catccccacc cgctttttcc gttttcctgt tttccgcaat ctgcaacgca
      601 gcaaccagtt tatgtagctg cgtatcgtct aaatattcga ccttatcctt accaaacatc
      661 cgccgcgcca ttgcgtgtgc atagttccaa tgtttgccgc cgacggtcag cagggcttcg
      721 actttgtcca acattgccgc tgatgatgtc cgacgcagat gcggtttgcc gtgtgggtta
      781 ccttttgctt taggcttaaa tccgtgcgac cgcatatcag cgacaacaga ctcaagttcg
      841 gaaacatcca tatccgcaca cgaccgcttg cccgtcacac gctccaacac cgcgcgatag
      901 gtaccgtcat ccaagcccag ctccttttga gcaatcttaa ttttcgcaat caacgcccgg
      961 cgcatctcaa acccataaaa cacaatatat agtattaagc cgatgttttt tgcgaaacgg
     1021 acagacataa aa
//
LOCUS       DAWIVB010000263         1022 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWIVB010000263 DAWIVB010000000
VERSION     DAWIVB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.263"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..>1022
                     /locus_tag="WIX60_002035"
     CDS             <1..>1022
                     /locus_tag="WIX60_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306237.1"
                     /translation="QSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKMGDLLLAAD
                     NLHSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQL
                     ALLDNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDK
                     AKAAIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAKNIYQNLLF
                     QTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRSW
                     YEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHNKNV
                     SLYGNDG"
BASE COUNT          323 a          224 c          227 g          248 t
ORIGIN      
        1 caatctttgg atctcgccta taaagatgta aataaaaatc tcggtaacgg taacactttg
       61 gctcagcaag gcagctatac caaaacagac ggtacaaccg caaaaatggg ggatttactt
      121 ttagcagccg acaatctgca cagccgcttc aaagacaaag tggaactcac tgccgaacag
      181 gcaaaagccg ccaatcttgc gggcattggt cgtctgcgcg atttgcgcga agctgccgca
      241 ttgtccggcg atttggccaa tatgctgaaa gcttattctg ccgccgaaac taaagaagca
      301 cagttggcat tgttagataa tttgattcac aaatgggcgg aaaccgattc gaactggggc
      361 aaaaaatcgc caatgcgact ttcaaccgat tggacgcaaa cggctaatga aggtattgca
      421 ctgacaccat cccaagtagc acaactaaaa aagaacgctt tagtttccct ttctgataaa
      481 gctaaagcag ctattgacgc cgcccgcgac cgcattgccg tgcttgatgc ctacacgggg
      541 caggattcca gcacactcta ttacatgagc gaagaagacg cgcttaatat cgtcaaagta
      601 accaacgata catacgacca tctcgccaaa aacatctacc aaaacctgtt attccaaacc
      661 cgtttgcagc catatttgaa tcaaatcagt ttcaaaatgg aaaatgatac gttcactttg
      721 gattttagtg gtcttgttca agcatttaac catgtcaaag aaactaatcc gcaaaaagct
      781 tttgtggatt tggccgagat gcttgcatat ggcgaacttc gttcttggta tgaaggccga
      841 agactaatgg ccgattatgt ggaggaggca aaaaaagcag gtaaatttga agattaccag
      901 aaagtgttgg gtcaggagac cgttgcatta ttagctaaaa catcgggtac gcaagcagat
      961 gatatcctgc aaaatgtagg ctttggtcat aataaaaatg tttctttata tggtaatgac
     1021 gg
//
LOCUS       DAWIVB010000264         1017 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWIVB010000264 DAWIVB010000000
VERSION     DAWIVB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.264"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            12..1013
                     /locus_tag="WIX60_002036"
     CDS             12..1013
                     /locus_tag="WIX60_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ5306238.1"
                     /translation="MIDRQTNEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
BASE COUNT          282 a          274 c          267 g          194 t
ORIGIN      
        1 ctacccaaat catgattgac agacaaacaa acgaacccaa acaaaaaacc cgaatcatcc
       61 ttgcccctat gcagggtctg gtcgatgacg tgatgcgcga cctgctgacg cgtattggcg
      121 gctacgacga atgcgtcagc gaatttgtac gcattaccca taccgtgcat tcccgatcca
      181 tatggttaaa atatgtcccc gaaatcgcca acggaaacaa aacgttttcc ggcacgccct
      241 gcaccgtcca acttttgggc agcgatgcgg acaatatggc ggcgaatgcg ctggaagccg
      301 tccgcttcgg tgcgaacaaa atcgatttga acttcggctg ccccgcgccc accgtcaaca
      361 aacacaaagg cggcgcaatc cttttaaaag agccggaact gatattccac atcgtcaaaa
      421 cgctgcgcgg acgtttgccc gcacatattc cgctcaccgc aaaaatgcgg ctcggttacg
      481 aagacaaaag ccgggctttg gaatgcgcct gtgcgattgc cgaagggggc gcatgcggac
      541 tgaccgtaca cgcgcgtacc aaagccgagg gttacgaacc gccggcgcat tgggaatgga
      601 taaggaaaat ccgagacagc gtcaatattc ccgttaccgc caacggcgac gttttcagcc
      661 tgcaagacta tatcggcatc aaaacaatca gcggctgcaa cagcgtgatg ctcggtcgcg
      721 gcgcggtcat ccgccccgat ttggcgcggc aaatcaagca atacgagaac ggcgggccgg
      781 tcaaagacac ggattttgcc gaagtttcca aatggatacg gcagtttttc gagctgtgcc
      841 tgacaaaaga ggcaaacaac aaatatccgc tggcgcggct gaaacagtgg ctgggtatga
      901 tgaagaaaga atttgcagca gcacaaaatc tgttcgaccg cgtccgaacg gttaaggatg
      961 cggacgaagt tcggaacatc ttggctgaat ttgagcgaga aatgaatact tgaatat
//
LOCUS       DAWIVB010000265          989 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWIVB010000265 DAWIVB010000000
VERSION     DAWIVB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.265"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            35..418
                     /locus_tag="WIX60_002037"
     CDS             35..418
                     /locus_tag="WIX60_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ5306239.1"
                     /translation="MYFFPRFPFIDIDRIRWVCAGLKAFKHYIRENGLPDLIHAHCMN
                     YAGILAFKISQKYGIPYVVTEHSSTITRGLVRPHQWQPMKNAAAHAAARLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            451..989
                     /locus_tag="WIX60_002038"
                     /pseudo
     CDS             451..989
                     /locus_tag="WIX60_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          245 a          293 c          242 g          209 t
ORIGIN      
        1 gcaaagcggt ttggacatct atgcatggca cggcatgtat ttcttccccc gctttccgtt
       61 catcgacatc gaccgcatcc gctgggtgtg tgcgggtttg aaagctttca aacactacat
      121 ccgcgaaaac gggctgcccg acctgattca cgcccactgt atgaactatg ccggcatact
      181 tgccttcaag atttcccaaa aatacggcat cccctatgtc gtcacggaac acagcagcac
      241 cattacgcgc ggtttggtgc gcccgcacca atggcagcct atgaaaaatg cggcggcaca
      301 cgccgccgca cgtctcgccg tcagccgcca tttcgcacac gtcctgcaac acaaatacgg
      361 ctgcgaatgg cagtacctcc cccaacatac tgggcggaat attcaaccga ccgtttgaac
      421 aaagagaaaa aaaacaacaa accgcatttc gtgttctgca ccgtctcgca ccttcgccgt
      481 ctcaaaggac acgatgtcct actcactgcc tttgcccggg cgttggcaca atgcccgcaa
      541 ctgcgcctga acatcggcgg cagcggacag gaagaacagc ggctgaaaca gcaggcggca
      601 gacttgggca ttacccatgc cgttacattt ttgggcgcat tgcagcccga agcagtcttg
      661 gatttgatga ggaacagcga cgcattcgtc cttgccagcc gcacagaaac cttcggcgta
      721 gtctacactg tcccaaggat tgcccgtcat tgcaacacgc tgcggcggtg cggaatctat
      781 tgtttcagac ggcaacggat atttggttcc tgttgacgac gacgatgccc ttgccgacgc
      841 actcatcaaa atgtgtgaac accactctga tttcgaacct gcccgactta gagaaaactg
      901 tctgaacgaa tttggcgaaa atgccgttat aggcaggttg atcggcattt tccgacaggc
      961 aatcgcagaa tacggtaaga aaataccgg
//
LOCUS       DAWIVB010000266          950 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWIVB010000266 DAWIVB010000000
VERSION     DAWIVB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.266"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            31..303
                     /locus_tag="WIX60_002039"
     CDS             31..303
                     /locus_tag="WIX60_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306240.1"
                     /translation="MDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQAIHEQPAFKP
                     YKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            348..638
                     /locus_tag="WIX60_002040"
     CDS             348..638
                     /locus_tag="WIX60_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306241.1"
                     /translation="MNIQLQGHIVGVKKINGQIEGKSFDYCCLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            643..840
                     /locus_tag="WIX60_002041"
     CDS             643..840
                     /locus_tag="WIX60_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306242.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
BASE COUNT          310 a          231 c          184 g          225 t
ORIGIN      
        1 gcaaacggtc tgaaattcat ttttcccgaa ttggacaaag ccaaactgtt tgaactgatt
       61 gagccgagtc atcacaagct gcccaagtct ttggctcccg aagcctacga ctgcgccttt
      121 ttgaaagctc aagccattca tgaacagccc gcattcaaac cgtacaaaga cccttactat
      181 atgtacgaat attacgagaa tcttgaaaaa cagcttgaac agcaaaaaca cgtcaacaat
      241 gaagaaagct ataacaactt catttacgac aaattcgcaa gactaccgat ttcatgggct
      301 taaagtgtct gcccgaaaga cgtttaatca cacaaggaaa ccaaaaaatg aacatccaac
      361 ttcaaggcca catcgtcggc gttaaaaaaa tcaacggaca aatcgaaggc aagagcttcg
      421 actattgctg cctgattgtc gccacaccct tagacagctc ccaaggcaac gcattgggca
      481 gctctactac tgaatacgat ttcggcggct ctgccaattt cgagcagttc cgaaacgccc
      541 aatttccgat cgaagcaaac ctgaacgtag aaatcgttac tacgggcaaa acccaaaaac
      601 tgaaagtcat cggttttcaa ctcgtgaaga aaggctgatt gaatgcagaa agtctatgtt
      661 gtccagtccg tatcaacagg ggactttctg tatctctctc ctgaaacggg cgacatcgga
      721 cataccaaat taatcaccaa tgccgattat ttctacgact tcgaagaagc gattaacgca
      781 ggtttggaag aaatcggcaa ccaatacgaa tttgtcgtat tcggattttt gaaagactga
      841 ttttcggatg ttcggcggtc gtctgaaaaa cgctccatcc attaccgcca aacacttttt
      901 gaaggaaaat atcatgaaat ttattaacac ctgccgtaaa tacggcgcaa
//
LOCUS       DAWIVB010000267          941 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWIVB010000267 DAWIVB010000000
VERSION     DAWIVB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.267"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            354..788
                     /locus_tag="WIX60_002042"
     CDS             354..788
                     /locus_tag="WIX60_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ5306243.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(836..>941)
                     /locus_tag="WIX60_002043"
     CDS             complement(836..>941)
                     /locus_tag="WIX60_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ5306244.1"
                     /translation="LRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
BASE COUNT          273 a          257 c          212 g          199 t
ORIGIN      
        1 ttgaacaaaa tgccgtccga acccttttca aagcgttcag acggcatttc atcaattata
       61 gtggattaac aaaaatcagt acggccttgc ctcgccttag ctcaaagaga acgattctct
      121 aaggtgctga agcaccaagt gaatcggttc cgtactgttt gtactgtctg cggcttcgtc
      181 gccttgtcct gatttttgtt aatccactat atttttggga atctgtttta ccccaatata
      241 taaagcacca tattaaggcg gagtgtcttc cccactttga cccgaacccg gaaaagacac
      301 cgcccaagcc aatcctgatg ctgccccgac agccaaccat taaggaaatc ctaatgaact
      361 ttgctttatc cgtcattatg ttgaccctcg cctctttcct gcccgtcccg cctgccggag
      421 ccgccgtctt tacttggaag gacggcggcg gcaacagcta ttcggatgta ccgaaacagc
      481 ttcatcccga ccaaagccaa atcttaaacc tgcggacgcg ccaaaccaaa ccggcggtca
      541 aacccgccca agccgacgca gggaagcgca cagacggcgc ggcacaggaa aacaatcccg
      601 acactgccga gaaaaaccgg cagcttgagg aagaaaagaa aagaattgcc gaaaccgaac
      661 ggcagaacaa agaagaaaac tgccggattt caaaaatgaa cctgaaggcg gtgggaaatt
      721 caaatgcaaa aaacaaggat gatttgattc ggaaatacaa taacgccgta aacaaatact
      781 gccgttaatc ggctctagcg caaacccgat gccgtctgaa gcggcacggg gtttgtcatt
      841 tctgccagta ggttttgacg ttgacgaact cgtacagccc gaattcggac aattcgcgcc
      901 cgtaaccgga atctttgact ccgccgaaag gcaggcgcaa a
//
LOCUS       DAWIVB010000268          913 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWIVB010000268 DAWIVB010000000
VERSION     DAWIVB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.268"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            313..873
                     /locus_tag="WIX60_002044"
     CDS             313..873
                     /locus_tag="WIX60_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ5306245.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          256 a          259 c          183 g          215 t
ORIGIN      
        1 ctataccacg atgaatcctt cgcaatatct gtttatcgac ctcaattttg acaaaatacc
       61 ggatacgcgc ctttgttgct tttccatctt ccaaccaact gtaaatctca aacagccggt
      121 acacgccatg cttcagtttc ttttcctgtc ggcggattgt ttcgacaaag aattgaaaat
      181 ccatttcatg caccttaaaa tttaatctgc attcaaacct tttcactttg gaagcaccat
      241 ttatcggatg tcccttcgca ataaacaaat tttcccgata ccgccgccca tttcaaccca
      301 aacccaaaag ctatgaaaaa cctcatcgcc ttcaacaaac cctatggcgt tatctgccaa
      361 ttttcaccgc acgaaaaaca caaaagcctc aaagacttta tcaatcttcc cggcttctac
      421 cccgccggac ggctcgacac cgacagcgag gggctgctgc tgctgaccga cgacggcagg
      481 cttcaggcac aaattaccga ccccaaattc aaacacccta aaacctactg ggcgcaactg
      541 gagggcgtac ccgacgaaag ccgattggaa agcctaagaa aagggataga cttaggcggt
      601 ttcgttaccc gtccggcaag catccgcatc ttgaaacacg gagaagcaga ttcgttatgg
      661 gagcgcatcc cgccgatacg cgtccgcaaa accgttcccg atttttggat tgaaattacc
      721 atttctgagg gcaaaaaccg ccaagtcagg cgaatgaccg ccaaggcggg ctatccctgc
      781 ctgcgtctga tcagagtggc aagcggcagg ctgaaactgt ttgatttgga tttaaaaccc
      841 ggggaatggg catacgcccc gtttaaacca taatcacgtt tatctcatca tttccacaaa
      901 agtgggaatc cgg
//
LOCUS       DAWIVB010000269          912 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWIVB010000269 DAWIVB010000000
VERSION     DAWIVB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.269"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(90..722)
                     /gene="mtrR"
                     /locus_tag="WIX60_002045"
     CDS             complement(90..722)
                     /gene="mtrR"
                     /locus_tag="WIX60_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ5306246.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          203 a          203 c          241 g          265 t
ORIGIN      
        1 atatgtattt ttagaagaag gctttgtgtc attttgatgc cgtctgaaag ggtttgttcg
       61 tttcagacgg cattgctacc aaggcttgat tatttccggc gcaggtcggg atggttttcc
      121 aagttgtcca tcattatccc gatgatgcgc ggggcggttt tgcccaaatc gaaacgttcg
      181 caggaagaga accaccgcca aatcagcccg tccaaggttg atttgatgaa gataactgcc
      241 gtttccttgt ccaaatcgtc agccaaatcc tgattttcca ccgcttcggt caaaacggcg
      301 gtaattttct cgcgccagat tgcctgatgc ttgcgggcaa tggcgataac ggcggcgttt
      361 tgctccgtgt gttcgcattt taaaaacagg atgttgtgga atttgtagta gatgtcgttg
      421 ctttgcagcc gctcgaaaaa gtgcagcagc gtgtggcgga ataccgccca agaccctcct
      481 tcggcatctt cggcatcttg cgcgatgcag ttttcgatgt cgtcgcagat acgttggaac
      541 agcgcgtcga acaagtcttc cttatttttg aaatgccaat agagcgcgcc gcgcgttacg
      601 ccggcggctt gggcgatttc gttgagcgag gtgcgcgcaa tccctttgcg gtaaaaggtt
      661 tccaaggcgg caagcatcag gtgttctttg gtttttaagg cttcggtttt ggtttttctc
      721 ataatggcgg tttcgtttcg ggtcggtttg atgagggcgg attataaaaa agactttgta
      781 accatgcaat cgtgtatgta taatgaaacc catgaaattg agactacatc tcaactttga
      841 aaacccatga aacctgcttc gcaacccgtt tgaacatcgg gttggcgaag caggcggttt
      901 ttatatcctg aa
//
LOCUS       DAWIVB010000270          908 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWIVB010000270 DAWIVB010000000
VERSION     DAWIVB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.270"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(175..333)
                     /locus_tag="WIX60_002046"
                     /pseudo
     CDS             complement(175..333)
                     /locus_tag="WIX60_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            489..>908
                     /locus_tag="WIX60_002047"
     CDS             489..>908
                     /locus_tag="WIX60_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306247.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PFGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQATILQTGN
                     GIPQVNIQTPTSAGVSVNQYAQFDVGNRGAILNNSRSN"
BASE COUNT          282 a          186 c          177 g          263 t
ORIGIN      
        1 cgtccaaaat gctctttggg aatgctattg gattatagac cttacaacag aaaaaaaggt
       61 cggaaagtag aaagactgct ttttaaattc aaacggccaa gcaagtaaag acttggccaa
      121 caaatgggac aaaatccgac tgttttaaat tgccagtcaa atactggaaa acttgcgtaa
      181 atggctaaat tcactgacat caagtacatc ataactacga aaggtatccg tatacacaat
      241 gccatcagac ttaactttct ctcggataat cggcaataat gttgctgatt gcgcattagg
      301 gacaacgacg gtataaacct tgccatttcg ttcgaatggg atttttaacc gttatctagt
      361 aaagtcccta atttaataaa caaatggcag taaaatggcg gtaattgact cgccaaaatc
      421 cttgatctag aatggtcaga ctatccaatt ttttctaata actctataaa aatcaaataa
      481 ctgaaatcat gaataaaact ctctatcgtg taattttcaa ccgcaaacgt ggggctgtgg
      541 tagccgttgc tgaaactacc aagcgcgaag gtaaaagctg tgccgatagt gattcaggca
      601 gcgctcatgt gaaatctgtt ccttttggta ctactcatgc acctgtttgt cgttcaaata
      661 tcttttcttt ttctttattg ggcttttctt tatgtttggc tgtaggtacg gccaatattg
      721 cttttgctga tggcattatt gctgataaag ctgctcctaa aactcaacaa gccacgattc
      781 tgcaaacagg taacggcata ccgcaagtca atattcaaac ccctacttcg gcaggggttt
      841 ctgttaatca atacgcccag tttgatgtgg gtaatcgcgg ggcgatttta aacaacagcc
      901 gcagcaac
//
LOCUS       DAWIVB010000271          906 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWIVB010000271 DAWIVB010000000
VERSION     DAWIVB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.271"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..216
                     /locus_tag="WIX60_002048"
     CDS             <1..216
                     /locus_tag="WIX60_002048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306248.1"
                     /translation="ADAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQG
                     NKPLGAEELADLKSLYKDLKNNIGNIV"
     gene            547..>906
                     /locus_tag="WIX60_002049"
     CDS             547..>906
                     /locus_tag="WIX60_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306249.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          282 a          147 c          212 g          265 t
ORIGIN      
        1 gctgatgccg gagacgtata ttgcgtctat aacatcagac tgaagcagta cactgcctgc
       61 caggttaccc gagttgaaga acacggtggc aaaaaaacac atgcgaccct gctggctttg
      121 gactggcagg gcaacaaacc gcttggggcg gaggagctgg cggatttgaa atcgctttac
      181 aaagacttaa agaataatat tggaaatatt gtatgaacaa aaaattaaac tatattttta
      241 tgttggactg tttagggttg gtgatattgt ttacttgtat aatagctact tttgaaagag
      301 attatggatt taaaattttt actaattcta agagacctga attttattat tggattggaa
      361 tgttttatta tggaattatt tcttgctggt ttgattatca attaatttca acaaaggcga
      421 attcgtataa aagaaaagtt aaacaatata aaattttttc agtaatattt tcagttttga
      481 tatttatttc tactatagta aaactttaaa ttttggagca aaaatttatg agcgattcaa
      541 ttgaatatgt attgggaacg cggtctgcac atgtataagg caagtgccgt cgtgccgacg
      601 ggatatgtac gggttgggaa taccgcgccg ctggtcggcg aagacacgca acggtatgcc
      661 tctttttggg gcgacggcta cgacgtgtac cgtcagttga gatggcagca gatacccgaa
      721 aaacagagaa aggcattcaa aaaagccgcc aaaagcaaaa agaccgtgat gtttgccgga
      781 cgggaatacg gcatatccaa acagaatttg agcgatgttt gggatgattt tgaagacgcg
      841 atggaactga aggcgtttcc ctgcctgtct tcgctgtttc tgaccaaatg gcataaaaat
      901 ctatat
//
LOCUS       DAWIVB010000272          867 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWIVB010000272 DAWIVB010000000
VERSION     DAWIVB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.272"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            102..503
                     /locus_tag="WIX60_002050"
     CDS             102..503
                     /locus_tag="WIX60_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ5306250.1"
                     /translation="MNTTRLPTALVLGCFCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKSKKN"
     gene            complement(566..856)
                     /locus_tag="WIX60_002051"
     CDS             complement(566..856)
                     /locus_tag="WIX60_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306251.1"
                     /translation="MAKYFTETGISVREHFDFFGEFVVSPAARSGDLALTYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVEGGRIDIGGFMLTAKEVDGGGNIGSMGLKVLR"
BASE COUNT          263 a          269 c          176 g          159 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctatactact gattcggcaa aaaaatcttt cacccgctac
       61 gcaaaatcaa caacaaggtt tcaaacatga aggaaacacc aatgaacaca acccgactgc
      121 cgaccgccct cgtcttgggc tgcttctgcg ccgccgcttc tgccgccgac aacagcatca
      181 tgacaaaagg gcaaaaagtg tacgaatcca actgcgtcgc ctgccacggc aaaaagggcg
      241 aaggccgcgg aaccatgttt ccgccgctct accgctccga cttcatcatg aaaaaaccgc
      301 aggtgctgct gcacagcatg gtcaaaggca tcaacggtac aatcaaagtc aacggcaaaa
      361 cctacaacgg attcatgccc gcaaccgcca tcagcgatgc ggacattgcc gccgtcgcca
      421 cttatatcat gaacgccttt gacaacggcg gcggaagcgt taccgaaaaa gacgtaaaac
      481 aggcaaaaag caaaaaaaac taaacagaca aaatgccgtc tgaaaccggc aatccggctt
      541 cagacggcat tcaaatcaaa cctttctaac gcagcacttt cagccccata gacccgatat
      601 tgccgccacc gtcaacctcc tttgcggtca gcataaagcc gccgatgtca atacggccgc
      661 cctcgaccgg ctcctgacta tcggaacgct tatcgaaaag ctcggcaagg ctcaaaccct
      721 cttcgcccgc ttccagcctc aaaccgtaag taagtgccaa atcacccgaa cgtgctgccg
      781 gcgaaacgac aaactcaccg aagaaatcaa aatgctcacg gacgctaata cccgtctcgg
      841 taaagtattt cgccatttta tagtgaa
//
LOCUS       DAWIVB010000273          855 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWIVB010000273 DAWIVB010000000
VERSION     DAWIVB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.273"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(78..362)
                     /locus_tag="WIX60_002052"
     CDS             complement(78..362)
                     /locus_tag="WIX60_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ5306252.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          245 a          230 c          150 g          230 t
ORIGIN      
        1 ttagaagttg cccgaaacct caaaaaaaaa aaccgaaacc gtacaagccg gattcccgcc
       61 tgcgcgggaa tgacgggcta aataatatca aaccataaat cctgccaaga aacattattt
      121 tcttcaatca gttgcaattt ccaagccctg ttccatttct tcaactgttt ttcccgagta
      181 attgcgctct ccatcgtagg atgcagttca taccaaacca gcatagtaac gttgtaccgt
      241 gatgtaaatc cctcaatcaa atgttcccta tgttggtaaa tacgttgcac caaatcagat
      301 gtaacgccaa tgtataacgt gccattacgt tggcttgcta aaatataaac cgcaggctgc
      361 atataacacc cttttgaatt atttaaattt atattcccgc gaacaccatc ccgtgattac
      421 tttaaccctt cgttattccc atagctttcc atcattcccg caactcttcg tcattcccgc
      481 gaaagtggga atctagaacg caaaatctaa agaaaccgtt ttacccgata agtttccgca
      541 ccgacaaacc tagattcccg cctgcgcggg aatgacggcg gagcggtttc tgttttttcc
      601 gataaatgcc gcaatctcaa atcccgtcat tcccacgaaa gtgggaatcc agaatctcta
      661 aagcttcagc taacctttga atattactgt tgttctaagg tctagattcc cgcctgcgcg
      721 ggaatgacga atccatccgc acggaaacct gcaccgcgtc attcccacga acctacatcc
      781 cgtcattccc acgaaagtgg gaatctagaa tctcggactt tcagataatc tttgaatatt
      841 gccgctgtcc aatgg
//
LOCUS       DAWIVB010000274          816 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWIVB010000274 DAWIVB010000000
VERSION     DAWIVB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.274"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(95..490)
                     /locus_tag="WIX60_002053"
     CDS             complement(95..490)
                     /locus_tag="WIX60_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306253.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(474..791)
                     /locus_tag="WIX60_002054"
     CDS             complement(474..791)
                     /locus_tag="WIX60_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ5306254.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
BASE COUNT          217 a          181 c          156 g          262 t
ORIGIN      
        1 tcactcccta attctaccca aataccccct taatcctccc cggatacccg ataatcaggc
       61 atccggggta ccttttaggc ggcaacaggc gcacttagcc tgagaccttt gcaaatttgt
      121 cggtttcggg gtcgtattgg tagcctcgtg cctgtatgtc ttctttgaaa gtttcgtata
      181 cgtcgtgggc taaaagggct gttccgacat agggaaccgc ccttgtgctg aatttcgcgc
      241 ctaagcgggc aagtttgccg acccccgcca atacgccggc gcgggatacg ctggcggtta
      301 ttttggcgtt gattcgggct tttgcgcccg tagggatgtg tgttaaatct accgttttta
      361 ttaaatcaga tgaataagtt ttactatttt taggtacaaa cttatgaatt ttcgcacctt
      421 gtccggtatc aactgaaaca gtttcagata tttttactgc atttgcattc gcttcaaacg
      481 aatacatcat caaaattgca attatcgaca atttcgcaaa attcaaattt gtatatttta
      541 tgaccatctt tcagagattc tttaattacc atttctgaat tatcagaaaa tgagattagc
      601 caaatatcat gtttaattct tctattccag aaaaaagaga aacaatcaat aacattttca
      661 gacttattaa tcttcgcaaa ttcaacaaat tcagattgcg ctataaccgc catcgattgc
      721 ccaaaatact tgctggacgg ctgatattta taaagtgcca actgcgcctg agtgataaac
      781 ggcttgttca tggttctgcc tttcaatgat tgtttt
//
LOCUS       DAWIVB010000275          759 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWIVB010000275 DAWIVB010000000
VERSION     DAWIVB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.275"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            620..>759
                     /gene="pilE"
                     /locus_tag="WIX60_002055"
     CDS             620..>759
                     /gene="pilE"
                     /locus_tag="WIX60_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HEZ5306255.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLA"
BASE COUNT          257 a          208 c          122 g          172 t
ORIGIN      
        1 aatcccgtca ttcccacgaa agtgggaatc cagaacgcaa aatctaaaga aaccgttttc
       61 ccgataaatt tccgtaccga cgaggctgga ttcccgcctg cgcgggaatg acgaagccat
      121 ccgcacggaa acctgccgcg atgattcaac tgccgtttgc acaggaacac cacgcgccga
      181 tttcaatcac tttccaagaa aacggaaatt tttaaaaatt aaatttaaaa tttcccaccc
      241 aacccaccct atgctaccgc gcaaattcaa aaatctcaaa ttccgaccca atcaacacac
      301 ccgatgcccc atgccaatag agatacccca cgccaataaa aaagtgccga aaatcggcac
      361 taaaactgac aattttcaac actgatgaca aaaaacggta accccctgct tcctcaaacc
      421 acgcccaaac gaaagaaaat gtaaaataaa aacaaccata taaagataaa cgcataaatt
      481 ttcacctcaa aacacaaact tgataccaat cttgctttat aatgcacagg tatcgtaaca
      541 aaataccgat ggtcaaatac attgcataat gccgatggcg taagcttgaa gcatttcctt
      601 tccaattagg agtaatttta tgaacaccct tcaaaaaggt tttaccctta tcgagctgat
      661 gattgtgatt gccatcgtcg gcattttggc ggcagtcgcc cttcctgctt atcaagacta
      721 cacagcccgc gcacaagttt ccgaagccat tcttttggc
//
LOCUS       DAWIVB010000276          758 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWIVB010000276 DAWIVB010000000
VERSION     DAWIVB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.276"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(1..104)
                     /locus_tag="WIX60_002056"
                     /pseudo
     CDS             complement(1..104)
                     /locus_tag="WIX60_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(141..632)
                     /locus_tag="WIX60_002057"
     CDS             complement(141..632)
                     /locus_tag="WIX60_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ5306256.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT          198 a          184 c          173 g          203 t
ORIGIN      
        1 cgggttttcg acaaatatcg gttgcgtttg gtttgcgcct ccgtcagcgg acggttgcgg
       61 caggctttgc gcataatgcc gttctgcaac cgatgctctt tcagttttcc gtaggtcgga
      121 ttctcgaatc cgacattact tcaatcgtat ccaatagaaa agtccgcatt gccgccaccc
      181 caattatgcg gataaatacc ctgtttgaca taacggtgaa acgtagaaaa cccccaatcg
      241 gaaatttgtc ctacatagcc atgtttgacc ggattgaaat gcagataatc aaaatgccag
      301 gcaaaatcgg cctcatcgcg gatagtatat tcccaaaagc gtttttgcca aagcctgaga
      361 ttgccgccga ttaaatattg gctgtgccgc ttgatttgcc gccagcgttc cgaataagca
      421 gaatcattgt ccggcagccg ccatatggta tgcagatggt cgggcatcaa cacccatgcc
      481 aaaatttcaa acggataccg ttcgcgcacc gccattaccg cctgccgtaa agccaaacgc
      541 accgcatcat cggtcaaaat cttctgccgt ttattggtta caaccgtaaa aaagtaagtg
      601 ccgccattgc ggtaaaaacg acggtatttc atagtattat gctcggaatg attttgtagg
      661 tcggattctt gaattcgaca ttttgggcat tgctgcaatg gattgcaatg atgggaatgt
      721 taaaggtttt gtcggataca agtatccgac ctacgctt
//
LOCUS       DAWIVB010000277          705 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWIVB010000277 DAWIVB010000000
VERSION     DAWIVB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.277"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(89..640)
                     /locus_tag="WIX60_002058"
     CDS             complement(89..640)
                     /locus_tag="WIX60_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306257.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          198 a          276 c          134 g           97 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc aagctgcggc tgccaaacac atcggctgct
       61 tctcgacgca cccgctgaaa aatgcgtcct atccgtccgc cttgcccgca ccccctgact
      121 ttgccgccct gcccgatgcc gtctgaagcc tgaaccgccc caccagcccg atgacgcacg
      181 acaccgccgg aaagggcata cacaaagccg tcagcacata acgcgcattc cccgtattga
      241 ccacgtccgc gcccttcagc agcagcggca gcgcgttcgc ccactcccac accgacggcg
      301 cgctgaacgc caataccgcc accagcccca acatccggaa caccccgatt tccaacacct
      361 gccgccgccg caccgaacgg ttgagcaaaa acacaaccgc caacccgaaa cagaccaccg
      421 ccgccatacc gccgttggcg acaatctgca aactgttgaa tgcaatatcg ggcgtaaccg
      481 ccaacacgcc ttccgaatcc ggcacggcac gctgcaaatc caaaccctgc aacacattca
      541 acacaaaacc gtagatcaaa tcaaaaaaca ccacgcctat cggcaaagca ctcaaaaccc
      601 gccacatctt cctgtccgac caaccgttca aaactttcat tttacccgac cacgcaagcc
      661 gccgaacaaa aaacaagggg ctgtcctaga taactaggac aaact
//
LOCUS       DAWIVB010000278          699 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWIVB010000278 DAWIVB010000000
VERSION     DAWIVB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.278"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
BASE COUNT          153 a          165 c          214 g          167 t
ORIGIN      
        1 agattgcagt gttgtcggga atgacgggtt tcaagattgc ggtgttgtcg ggaatgacga
       61 atccatccat acggaaacct gcaccacgtc attcccacgg aagtgggaat ctagaatccc
      121 ggggtttcag tcatttccga tagattcccg ccgcgtcggg ggtctagatt cccgcctgcg
      181 cgggaatgac gggtttcgag attgcggtgt tgtcggaacg caactgaacc gtcattccca
      241 cgacagtggg aatctagaat ctcgggggtt cagtcatttc cgatagattc ccgccgcgtc
      301 agggggtcta gattcccgcc tgcgcgggaa tgatgggttt caagattgcg gtattgtcgg
      361 gaatgacgaa tccatccata cggaaacctg caccacgtca ttcccacgaa agtgggaatc
      421 tagaatcccg gggtttcagt catttccgat agattcccgc cgcgtcaggg agtctggatt
      481 cccgcctgcg cgggaatgac gaatttcgag attgcggtat tatcgggaat gacgaatttc
      541 gagattgcgg tattgtcggg aatggcgggt ttcaagatta cggtgttgtc gggaatgacg
      601 gttcgggtat ttccacgccc gccccgcgcc tgtaaacggc aggtgaatca aaaatgccgt
      661 ctgaaggttc agacggcatc ggtgtcgggg aatcagaag
//
LOCUS       DAWIVB010000279          695 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWIVB010000279 DAWIVB010000000
VERSION     DAWIVB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.279"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..120
                     /locus_tag="WIX60_002059"
     CDS             <1..120
                     /locus_tag="WIX60_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ5306258.1"
                     /translation="NVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            138..449
                     /gene="rpsJ"
                     /locus_tag="WIX60_002060"
     CDS             138..449
                     /gene="rpsJ"
                     /locus_tag="WIX60_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ5306259.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            591..>695
                     /locus_tag="WIX60_002061"
     CDS             591..>695
                     /locus_tag="WIX60_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ5306260.1"
                     /translation="MIAKHIDRFPLLKLDRVIDWQPIEQYLNRQRTRYL"
BASE COUNT          196 a          177 c          152 g          170 t
ORIGIN      
        1 aacgtaacca tcaccgtaga actgattgcg cctatcgcta tggaagaagg cctgcgcttt
       61 gcgattcgcg aaggcggccg taccgtgggt gccggcgtgg tttcttctgt tatcgcttaa
      121 ttgaaggata ttgataaatg gcaaaccaaa aaatccgtat ccgcctgaaa gcttatgatt
      181 acgccctgat tgaccgttct gcacaagaaa tcgttgaaac tgcaaaacgt accggtgcag
      241 ttgtaaaagg cccgattcct ttgccgacca aaatcgagcg tttcaacatt ttgcgttctc
      301 cgcacgtgaa caaaacttcc cgtgagcaat tggaaatccg cacccacttg cgcctgatgg
      361 acatcgtgga ttggaccgat aaaactaccg atgcgctgat gaagctggat ttgccggccg
      421 gtgttgatgt agaaatcaaa gtccaataat tcggactata aaaaatcccc aagcaatcaa
      481 tgcttgggga ttttttatgt tatgccgaga cctttgcaaa attccccaaa atcccctaaa
      541 ttcccaccaa gacatttagg agcaccttct tccagcaaac cgcacaagcc atgattgcca
      601 aacacatcga ccggttccca ctattgaagt tggaccgggt aattgattgg cagccgatcg
      661 aacagtacct gaatcgtcaa agaacccgtt acctt
//
LOCUS       DAWIVB010000280          688 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWIVB010000280 DAWIVB010000000
VERSION     DAWIVB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.280"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            232..531
                     /locus_tag="WIX60_002062"
     CDS             232..531
                     /locus_tag="WIX60_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ5306261.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPTGMAVSSLPPQPH"
     gene            528..>688
                     /locus_tag="WIX60_002063"
     CDS             528..>688
                     /locus_tag="WIX60_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
                     /protein_id="HEZ5306262.1"
                     /translation="MTQRPYATLYLTAHYKIGKSTRIGLDFENVFNKRYRPMPDIHVY
                     GTPRSLTATV"
BASE COUNT          178 a          197 c          142 g          171 t
ORIGIN      
        1 tcaaacatca aattggactg accaatcagg gcggattcta atgacacgcc gttcccgccg
       61 tcaacggcat ttacctcgca ccgcccccga aacacaagaa aaaactacac caaactacaa
      121 tttttgttca tgcaaatatt tgttttgaca ggatttaaac aaaagctccg attcaaatct
      181 gccgaaccgc ccaaaaatat attgacctaa atattaaagt ttcgtaaagt aatgcaacgt
      241 tgctttaatt ggtttgacca ctattgccga cgattagaaa aatattttcg gagatgttca
      301 attatggaaa cttgggttca aaactacacg gcaatcggcg gcagcctgta tctgactgcc
      361 gccgccgcac tcttacccat cgtctttttc tttgccgcgc tgaccgtcct gaagctgaaa
      421 ggctatcagg cggggcttta tacgctgctg attgcgcttg ccgttgccgt attcggcttc
      481 gggatgccga cgggtatggc ggtttcttcc ctgccgccgc agccgcattg acccaacgcc
      541 cctacgccac actgtatttg accgcgcatt acaaaatcgg caaatccacc cgcatcggtt
      601 tggactttga aaacgtgttc aacaaacgct accgccctat gcccgacatt cacgtttacg
      661 gcacgccgcg cagcctgacc gcaaccgt
//
LOCUS       DAWIVB010000281          678 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWIVB010000281 DAWIVB010000000
VERSION     DAWIVB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.281"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(173..>678)
                     /locus_tag="WIX60_002064"
     CDS             complement(173..>678)
                     /locus_tag="WIX60_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ5306263.1"
                     /translation="WFCGQPVTRAGTDDTVAADNTGNKKTNTKHLPSTCRDAASAVCT
                     KTPEYYPNHGEWPKNNTSAGVASSSTIKGKYVKEVTVANGVITATMLSSGVNKEIQGK
                     KLSLWAKRQDGSVKWFCGQPVTRADNAKDDVAAATGTDKIDTKLKKPFYPISFRTDEA
                     GFPLLRE"
BASE COUNT          124 a          143 c          200 g          211 t
ORIGIN      
        1 gtcggtgcgg aaacttatcg ggaaaacggt ttctttagat tttacgttct ggattcctac
       61 tttcgtggga aagacgaatt ttaggtttct gtttttggtt ttctgtcctt gtgggaatga
      121 tgaaaattta agttttagga atttatcgga aaaaacagaa accgctctgc cgtcattccc
      181 gcaaaagcgg gaatccagcc tcgtcggtgc ggaaacttat cgggtaaaaa ggtttcttta
      241 gtttggtgtc gattttgtcg gtgccggtgg cggcggcaac gtcgtctttg gcgttgtcgg
      301 cgcgcgtaac cggctgtccg cagaaccatt ttaccgaacc gtcttgacgc ttggcccaca
      361 gggagagttt tttgccttgg atttctttgt ttacgccgct tgaaagcatt gtggcggtaa
      421 tgacgccgtt tgcgactgta acttccttaa catatttgcc tttgattgtt gaagaagatg
      481 ccacgccggc agaagtgttg tttttcggcc attcgccgtg attcgggtaa tactcgggtg
      541 tttttgtgca aacggcagat gctgcgtcgc ggcaggttga cggcaggtgc ttggtgttgg
      601 ttttcttgtt gccggtgttg tcggcggcga cggtgtcgtc ggtgccggcg cgcgtaaccg
      661 gctgtccgca gaaccatt
//
LOCUS       DAWIVB010000282          677 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWIVB010000282 DAWIVB010000000
VERSION     DAWIVB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.282"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            4..236
                     /locus_tag="WIX60_002065"
                     /pseudo
     CDS             4..236
                     /locus_tag="WIX60_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            295..603
                     /locus_tag="WIX60_002066"
     CDS             295..603
                     /locus_tag="WIX60_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306264.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMGASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK
                     "
BASE COUNT          211 a          164 c          153 g          149 t
ORIGIN      
        1 aaaatgttga aaacaaaaag aactcattgc ttgaaaaaag atttaatggg aatgcgggta
       61 tttcccacac tttttccatt tatttatcct cattatagga agaaaaacat gaaaaaaccc
      121 tgcttgcact ctcacttgca gccgtgtccg ccgttacggt agccgatatt cccgagtcta
      181 acggcaaagg caaagccatc atcggcgata aggaaaaaca ccgttaccaa gcatgaaaac
      241 cttgctttga aagtccatgg cgaaatccgc caagatttgg gggacggatg atccctgacc
      301 ccttgggcgg atgcatatgc agatttgcgc ggcaaaacca aagtgatgac gacccagatg
      361 ggtgcttccc gcgatgtcag caaaagcgcc aaaggttgga gtgtcggtat cggtctgaat
      421 gtaggcaaac agttgaccga cagcgtcggt ctcgagtttg atccatacta ccgtcacaaa
      481 acaatctaca aaccccgtga gattgtcttg gacggtgaca aaaccaaaat gggccgctcc
      541 aaatccaacg agtacggctt ccgcgtagcc gcaacgttct atagtcaatt aaaatcaaaa
      601 taggacagta gtgcatcgtc aaatcgggcg taatcagaca aaacggttcg cagataccgc
      661 ttaatattcg cccacac
//
LOCUS       DAWIVB010000283          663 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWIVB010000283 DAWIVB010000000
VERSION     DAWIVB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.283"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
BASE COUNT          191 a          161 c          171 g          140 t
ORIGIN      
        1 tcccgcgaac gcgggaatct agaacgtaga atctgagaaa ccgttttact cgataaattt
       61 ccgtgccgac aggtctggat tcccgccttc gcgggaatga cggcatcagt ttgcaggatt
      121 cggcggaaac ggtaaaaacg gcagaatcga tgggatgcgg caggctgaag cccaccaaaa
      181 cacaaaaatt ccgatgccgt ctgaaatttc gtcattcccg tgaaaacggg aatccagccc
      241 cgtgggagca gaaacttaca ccccgtcatt cccgcaaaag cgggaatcca gtaaccgaaa
      301 aaccacggga atctatcgga aaaaacagaa ccccccgccg ccgtcattcc cgcgaacgcg
      361 ggaatctaga acgtagaatc tgagaaaccg ttttactcga taaatttcca tgccgagggg
      421 tctggattcc cgcgttcgcg ggaatgacgg catatttttt gcatttgata taaagggtcg
      481 tttgaatttt gttcagcaag tgcaaagtgt tgcacataaa agggcgcagg atagaggcaa
      541 agcgggcgta ggtcgggctg tagcaactgt atttttcacc ccgtcgggca aaaatatagt
      601 ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa
      661 ggt
//
LOCUS       DAWIVB010000284          662 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWIVB010000284 DAWIVB010000000
VERSION     DAWIVB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.284"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(60..545)
                     /gene="fxsA"
                     /locus_tag="WIX60_002067"
     CDS             complement(60..545)
                     /gene="fxsA"
                     /locus_tag="WIX60_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ5306265.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          195 a          212 c          120 g          135 t
ORIGIN      
        1 actatatttt caaaccggaa aaatgccgtc tgaaccttca gacggcattc cattcatatt
       61 tattcgtctt ttttgtgttc gatggcgttt cgggaacgat tgccgccgta aggctcttca
      121 accgtatatt ctccctcgat aatatcgtca tcgcgggaaa agccctcttt tctgcccgat
      181 tggttcatgt tgaaaaaatt ttccgcacct cctgcctgca acactgcccc tcccttaaac
      241 ggcagcagca gcaataccgc caacaccgag gatacgaatc ccggactcat cagacacaca
      301 gccgccaccg tataacggat aggccacaac atctgataaa cggataccct cccgccgctt
      361 ctcattgccg cgcccgccaa taaaagaccg gacagccccg tatgcctgag catcagcacg
      421 ccggcggcaa aacctgccgc catcaaaaac aacgtccagc cgccgcccag ccaatcggca
      481 acccacacaa tcgacataat ctccaaaaac agcagcacca aaaaaccgat accgaaaaat
      541 ctcattgacc gtcatcctta tatttaagta aacagcaaac cgcccgaaca ggactccaag
      601 cgagctgcct gtaaatgatt acaaaaccat gtgcttcaag ccgaaacaat gtgaaatcac
      661 gc
//
LOCUS       DAWIVB010000285          627 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWIVB010000285 DAWIVB010000000
VERSION     DAWIVB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.285"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(181..273)
                     /locus_tag="WIX60_002068"
     tRNA            complement(181..273)
                     /locus_tag="WIX60_002068"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(237..239),aa:Ser,seq:gct)
     gene            complement(430..627)
                     /locus_tag="WIX60_002069"
     CDS             complement(430..627)
                     /locus_tag="WIX60_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306266.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
BASE COUNT          172 a          154 c          128 g          173 t
ORIGIN      
        1 cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggtttttg
       61 ttcatccact ataacaaaat tcagaatcaa gacagggcaa taccatactg gttttgcacc
      121 aacccgctat aaaattgcat aaaaaataga cgctgtaaaa cacagcgtct attttttatc
      181 tggcggagta agagggattc gaaccctcga tgcaggttga ccccacatgc ttccttagca
      241 gggaagtgcc ttcagcctct cagccattac tcctaaggaa gctgtaacta taatcaactt
      301 ttccgatacc gtcaaggaaa aacagaaagc aaatattaat cttttgaaat aaaataagat
      361 ttaccctgat tatacccaat caggcacttc ttttcttcct gaatggaaat attcctgact
      421 gccacatcct caagcaaaat gcttttgtgc atcctgtttg atttcctgaa accacgcgtc
      481 ttcaggaaaa cgtgcaagcg cgacatccaa tgttgacagc aggttttgcg acagggcttt
      541 ggtcaggtat tcctcctcgt tggcttctgc cgctttgtcg ggctgttgtg ccaaaccgtc
      601 ggcacgggct ttgtgcccgt gtgccaa
//
LOCUS       DAWIVB010000286          603 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWIVB010000286 DAWIVB010000000
VERSION     DAWIVB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.286"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(150..536)
                     /locus_tag="WIX60_002070"
                     /pseudo
     CDS             complement(150..536)
                     /locus_tag="WIX60_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          112 a          133 c          169 g          189 t
ORIGIN      
        1 ctgtttttgg ttttctgttt ttgagggaat gatgaaattt taagttttag gaatttatca
       61 gaaaaaacag aaaccgctcc gccgtcattc ccgcgcaggc gggaatccag gtctgtcggt
      121 acggaaactt atcgggtaaa acggtttctc tagtttggtg tcgattttct tgtcggtgct
      181 gttgacggca ggtgcttggt gttgatgttg gcggtgccct tgccggtggc ggcggtgacg
      241 gcgtcgtctt tggctttgtc gcgcgtaacc ggctgtccgc agaaccattt taccgaaccg
      301 ttttgacgct tggcccacag ggagagtttt ttgcctttga tttcgttgtt tacgttgctt
      361 gaagccattt gggcggtaac gacgccgttt ttgacttcaa cgcttttaac atatttgcct
      421 ttgatttcag aggaggttgc cacgccggca gaactgttgt cgccgggcca ttcgccgtga
      481 ttcaggtaat actcggtaac ggctgatttt tgaccttcga ccaaaaggat agcttcgtca
      541 ttcccgcgca ggcgggaatc cagccttgtc ggtacggaaa cttatcgggt aaaacggttt
      601 ctt
//
LOCUS       DAWIVB010000287          585 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWIVB010000287 DAWIVB010000000
VERSION     DAWIVB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.287"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
BASE COUNT          142 a          157 c          131 g          155 t
ORIGIN      
        1 aacccgaaac ggcaacttaa aacccccgtc attcccgcgc aggcgggaat ccagtccgtt
       61 cggtttcagt catttccgat aaattcctgt tgcttttcat ttctagattc ccacttttgt
      121 gggaatgacg gcggagggtt ttttgttgtt tcggagaagt ttctgcaacc ctagaatctc
      181 gttatttcca caaaaaacag caacccaaaa cggcaactta aaaccccgtc attcccgcgc
      241 aggcagaaat ccagtccgtt cagtttcggt catttccgat aaattcctgt tgcttttcat
      301 ttctggattc ccacttttgt gggaatgacg gcggaaggga taaatcctcg caatccaaag
      361 cctcgtcatt tctacaaaaa acagcaaccc gaaacagcaa cttaaaaccc cgtcattccc
      421 gcgcaggcgg gaatctaggt ctgtcggttc aggaacttat cggataaaac ggtttctcca
      481 accctgcgtt ctagattccc actttcgtgg gaatgacggg atatgggttt ccgtgcggac
      541 gtgttcggat ttccgcctgc gcgggaatga cggcgacaga tgccc
//
LOCUS       DAWIVB010000288          585 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWIVB010000288 DAWIVB010000000
VERSION     DAWIVB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.288"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            100..387
                     /locus_tag="WIX60_002071"
     CDS             100..387
                     /locus_tag="WIX60_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306267.1"
                     /translation="MDDLIAAFLGYLSGKYREGKISRLGISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITEMMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          199 a           70 c           99 g          217 t
ORIGIN      
        1 ccaaagaggc tgaagggtaa gagacgctta tatcatagat atagtggcaa aacaggtaat
       61 gaattaaaga cattacctgt ttttacagaa atttttaaaa tggatgattt gattgcagct
      121 tttttaggct atttatcagg taaatataga gaaggaaaaa taagcaggtt aggaatatcc
      181 ctaacggcag gaagcatttt ctttgcagta ttctttattc atgaactgat aatttttatt
      241 gttgatggaa ataaggaaaa tctttggaat tttattactg agatgatgaa agatttattt
      301 ttattttcat gttctgtttg tttattattc tacttcttat tatttattgc ggaaactatt
      361 gggaaaacaa tcaataaaat taaataagat atttaagccg ctaaaaacca tttaagtatt
      421 ttatcttcta tgcggcattt atcttggaat tgaaatactg caatcgtgag gctaaggctt
      481 ccgttgtaac gtttaccata tatttcatta ttttaatttc atcaaaaaac aaaattcaag
      541 attatacata ccgccaatag tttcttgatt gttttgtata gtgga
//
LOCUS       DAWIVB010000289          580 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWIVB010000289 DAWIVB010000000
VERSION     DAWIVB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.289"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            complement(300..>580)
                     /locus_tag="WIX60_002072"
     CDS             complement(300..>580)
                     /locus_tag="WIX60_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ5306268.1"
                     /translation="NIKGKYVEKVEVKNGVVTATMLSTGVNKEIKGKKLSLWAKRQDG
                     SVKWFYGQPVKRDNASADAVKADTAANGKQIDTKHLPSTASTRKSTPN"
BASE COUNT          121 a          120 c          152 g          187 t
ORIGIN      
        1 tctgtcggca cggaaactta tcgggaaaac agtttcttga gattttgcgt tctggattcc
       61 cgctttcgcg ggaatgacgg gattaaagtt tcaaaattta ttctaaataa ctgaaattca
      121 acgaactaga ttcccacttt cgtgggaatg acgaatttta ggttgctgtt tttgtgggaa
      181 tgatgaaatt ttaagtttta ggaatttatc gaaaaaacag aaaccgctcc gccgtcattc
      241 ccgcgcaggc gggaatccag cctcgtcggt acggaaactt atcgggtaaa aaggtttctc
      301 tagtttggtg tcgattttct tgtcgatgct gttgacggca ggtgcttggt gtcgatctgc
      361 ttgccgttgg cggcggtgtc ggctttgacg gcgtcggcgc tggcgttgtc gcgcttaacc
      421 ggctgtccgt agaaccattt taccgaaccg tcttgacgct tggcccacag ggagagtttt
      481 ttgcctttga tttctttgtt tacgccggtt gaaagcattg tggcggtaac gacgccgttt
      541 ttgacttcaa ctttctcaac atatttgcct ttgatgttgg
//
LOCUS       DAWIVB010000290          556 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.290, whole genome shotgun sequence.
ACCESSION   DAWIVB010000290 DAWIVB010000000
VERSION     DAWIVB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.290"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
BASE COUNT          164 a          155 c          114 g          123 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
       61 ctatatccgt ttgcgcccgc caagccgtgt tgcacagatg cgacacggct tttttctttc
      121 tgttgccaga atgtaaattt tcgtcaaaaa acatcaaaaa tcaccccccc cccgaatttg
      181 cgtaaaataa agtgtaatcc ctttgatcat aagggaatgt tgacaattag atcgattctc
      241 ctaggctgac tttcgcaata aatggggttg tcaataattg tgtatggtcg tttgccgtcc
      301 ggcggccgtc attcccgcga acgcaggaat ccagcaacac aatgcagcag gactttatcg
      361 gaaaaaaccg aacccccacc gactgtcatt cccgcaaaag ctggaatcca aaaacgcaac
      421 gcaacaggaa tttatcggaa aaaacagaac ccccccaccg ccgtcattcc cgcgcaggcg
      481 ggaatccaga aacacaacgc ggcaggactt tatcggaaaa aaccgaaacc ccaccgaccg
      541 tcattcccgc aaaagc
//
LOCUS       DAWIVB010000291          555 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.291, whole genome shotgun sequence.
ACCESSION   DAWIVB010000291 DAWIVB010000000
VERSION     DAWIVB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.291"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            321..491
                     /locus_tag="WIX60_002073"
     CDS             321..491
                     /locus_tag="WIX60_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ5306269.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPRTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT          177 a          154 c          129 g           95 t
ORIGIN      
        1 ccctaacgca acaggaattc atcgaaaaaa ccgaaacctc caaaccgtca ttcccacgga
       61 agtgggaatc cagtaatcga aaaaccacag gaatctatcg aaaaaaacca accccaaaaa
      121 aaccgggcgg ataccgcacc attctcccaa accctgattt atagcggatg aacaaaaatc
      181 agggcaaggc ggcgaagccg cagacagtac aaatagtacg ggtgcttcag tactttagag
      241 aatcgttctc ttcgagctaa ggcgaggcaa cgccgtaccg gtttttgtta acccactata
      301 caaaaaacaa ggaaacacaa atggcgcaat atatgtgcgg cccctgcggc tggatttacg
      361 atgaagaaca cggcgacccc gaacacggca tcccccctag gacaaagttt gaagacatcc
      421 ccgacgactg gaaatgcccc gaatgcggcg taggtaaaga agatttctac ctgttggatt
      481 tcgtgatata gcggcagccg cacccttagg gaaaaccccg tcatccggcg ttccgcctgc
      541 ggcggcaggg aacac
//
LOCUS       DAWIVB010000292          547 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.292, whole genome shotgun sequence.
ACCESSION   DAWIVB010000292 DAWIVB010000000
VERSION     DAWIVB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.292"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            <1..>547
                     /locus_tag="WIX60_002074"
     CDS             <1..>547
                     /locus_tag="WIX60_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ5306270.1"
                     /translation="ATGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAADYASY
                     RKWNNNKYSVNTKELQKNNSSGIWQELKTENQENGTFHAASSLGLSAIYDFKLNDKFD
                     KFKPYIGARVAYGHVKHQVHSVRKETTTTFSPPAQGATVPGKIVQGPTNKPAYHESNS
                     ISSLGLGVIAGVGFDITPKLT"
BASE COUNT          174 a          144 c          117 g          112 t
ORIGIN      
        1 aagcaaccgg tgcaaacaac acaagcacag taagcgatta tttcagaaac atccgtgcgc
       61 attccatcca cccccgggtg tcggtcggct acgatttcgg cggctggagg atagcggcag
      121 attatgccag ttacagaaaa tggaacaaca ataaatattc cgtaaacaca aaagagttgc
      181 aaaaaaacaa tagcagtggc atctggcaag aactgaagac ggaaaatcag gaaaacggta
      241 cattccacgc cgcttcttct ctcggcttat ccgccattta cgatttcaaa ctcaacgata
      301 aattcgataa attcaaaccc tatatcggtg cgcgcgtcgc ctacggacac gttaaacatc
      361 aggttcattc ggtgagaaaa gaaaccacga ctactttcag tccaccagcg caaggcgcta
      421 cagtgccagg caaaatcgta caaggtccga ccaacaaacc tgcctatcac gaaagcaaca
      481 gtatcagcag cttaggtctt ggtgtcatcg ccggtgtcgg tttcgacatc acacccaagc
      541 tgacttt
//
LOCUS       DAWIVB010000293          541 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.293, whole genome shotgun sequence.
ACCESSION   DAWIVB010000293 DAWIVB010000000
VERSION     DAWIVB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.293"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
     gene            282..461
                     /locus_tag="WIX60_002075"
     CDS             282..461
                     /locus_tag="WIX60_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ5306271.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
BASE COUNT          132 a           99 c          151 g          159 t
ORIGIN      
        1 tgctcgatcg gctgccaatc aatcacctgg tccaacttca atagtgggaa acggtcgatg
       61 tgtttggcaa tcatggcttg ggcggtttgc tggaagaagg tgctcctaaa tgtcttggtg
      121 ggaatttagg ggattttggg gaattttgca aaggtctcta tttgtactgt cttcggctcg
      181 ccgccttgtc ctgatttaaa tttaattcac tatatttgcc cgtgcgtttg tgataaattc
      241 ggcactgtat ttgatatgat tttaacaaat cgggaggggc aatgttttgg tatgtgattg
      301 gcttttgcgc ctttgtcgtc gcgctgctgt cgctgtgggt caatgccggc gcgttcggta
      361 tgcaggaaga cgatactccg caatcggatt atgaaaggcg tttgggcttg ggtgcgaagc
      421 tgaaaaacaa aaatccggat acggacgaaa aacggcaata aagacaacaa atgccgtctg
      481 aaaggttttc agacggcatt tgctttggag cggatatagt ggattaacaa aaatcaggac
      541 a
//
LOCUS       DAWIVB010000294          521 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M36722
            SAMN14500827-rid9852023.denovo.294, whole genome shotgun sequence.
ACCESSION   DAWIVB010000294 DAWIVB010000000
VERSION     DAWIVB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14500827
            Sequence Read Archive: SRR11454966
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000739083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000739083.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 22:04:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,075
            CDSs (total)                      :: 2,026
            Genes (coding)                    :: 1,930
            CDSs (with protein)               :: 1,930
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 39 of 96
            Pseudo Genes (incomplete)         :: 54 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14500827-rid9852023.denovo.294"
                     /isolate="M36722"
                     /isolation_source="nasopharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2015"
                     /collected_by="US State Health Department"
BASE COUNT          155 a          118 c          105 g          143 t
ORIGIN      
        1 tttttttgaa tttcgggcaa cgcttgaatc ttcattccgc gcaggcggaa attatcggtg
       61 cggtacggca acttttttcg atatgaaaag accgtcattc ctgtaaaaac aaaaaatcaa
      121 aaacagaaaa ttgaaattcg tcattcccgc gcaggcggga atccaggacg taaaatctat
      181 agtggattaa caaaaaccag tacagcgttg ccttgactta gctcgaagag aacgattctc
      241 taaggtgctg aagcaccgag tgagtcggtt ccgtactatc cgtactgtct gcggcttcgt
      301 cgccttgtcc tgatttttgt taatccacta taaagaaacc gtttttctcg ataagtttcc
      361 gtgccgacag acctggattc ccactttcgt gggaatgacg gtggaaaagt tgccgtgatt
      421 tcggataaat tttcgtaacg cataatttcc gttttacccg ataaatgccc gcaatctcaa
      481 atcccgtcat tccccaaaaa caaaaaatca aaaacagaaa t
//
